LOCUS       DASQTF010000001         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_556148,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000001 DASQTF010000000
VERSION     DASQTF010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556148"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VE308_00005"
     CDS             <1..716
                     /locus_tag="VE308_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99383.1"
                     /translation="KKDLGTAESVATEYIEVKEGMVSKKHYYIPKYYIQGFDGDSLYT
                     SLIKDEIKDRFERDSPPSPSESQTPEYQEQVRQVESAHPQFVHGVPFMAKEPSTEISV
                     DYSGTTYNIPWDQIIHKHARTTDKVEIGYVERIGNEFIVVREGVSNVHIYYIPKTYIR
                     DYDGSQLWIDAPSGLVRSKFERETEPPIEELRAMAIEAPRYRRVEPQMQRKEETDTVM
                     VETDIDLEQSSTEKNTERA"
     gene            complement(730..1431)
                     /locus_tag="VE308_00010"
     CDS             complement(730..1431)
                     /locus_tag="VE308_00010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HZA99384.1"
                     /translation="MSLSLVFTDNTTIRIDICPNKRTKRLRLVSGINGVRAMVPLNYN
                     AEELESFVASKRNWILKTSQYYSKLKERCGGLEPYAIYFLGSKYRFHVVKDRLPSTVI
                     TDILKLITFHVTDMRRYKQHMREWYKQQTARIIADRLPVLASTFNVKYNKVSIKNQKS
                     RWASCSKTGNLHFNLLLVAAPPNVIDYVMIHELIHLIEFDHSRQFWQLVKEADPDYQK
                     HREWLANYGSVIKVV"
     gene            1809..>2036
                     /locus_tag="VE308_00015"
     CDS             1809..>2036
                     /locus_tag="VE308_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99385.1"
                     /translation="MVTGKYFNKGVKSLDSPHVGHVVRETSDKIVVFGEGDDRYDIPK
                     DKIRFAAANVLIDLPFYEIVKNYKVSREEPLP"
BASE COUNT          615 a          405 c          456 g          560 t
ORIGIN      
        1 acaagaaaga tctcggcacc gcagaaagtg ttgcaactga gtatatcgag gtcaaggagg
       61 gaatggtgag caaaaagcac tactacatcc ccaagtatta cattcaaggc tttgacggcg
      121 acagcttgta cacctccctc ataaaggatg agatcaaaga tcgctttgag cgcgatagtc
      181 caccatcgcc ttcagagtca caaactccag aataccaaga gcaggtgcgc caagtagaat
      241 ctgctcatcc gcaatttgtt cacggtgtgc ctttcatggc aaaggaaccc tcgactgaga
      301 tttcagtgga ctactctggt acaacataca acataccatg ggatcagatt attcacaagc
      361 atgccaggac gacggataag gtagagatag gatacgttga aaggattggc aatgagttca
      421 ttgtggtaag agagggtgta tcaaacgtcc acatctatta tattcccaag acgtatatcc
      481 gcgactacga cggaagccag ctatggatag atgcgccgag tggtcttgtc agatccaagt
      541 ttgagagaga gactgagcct ccaattgagg agcttcgggc catggcaata gaagccccgc
      601 gttatagaag ggtcgagccg cagatgcagc gcaaagaaga aaccgataca gtcatggtag
      661 aaacagacat tgacctagag cagagcagta cagaaaaaaa tacagagcgt gcctaacctg
      721 cgctaccttt tacactactt ttattactga cccgtaattg gcaagccatt ctcggtgctt
      781 ctggtaatca gggtcagctt cctttactaa ctgccagaat tgtcgtgagt ggtcaaactc
      841 tattaggtgt ataagctcgt ggatcataac atagtcaatg acattgggtg gagctgctac
      901 tagcagaagg ttgaaatgca ggttcccggt ttttgagcag ctggcccatc tcgacttttg
      961 attctttatc gatactttgt tatacttgac gttaaacgta cttgcaagga ctggtaggcg
     1021 gtctgcaatt attctggcag tctgctgttt gtaccattcg cgcatatgct gtttatatct
     1081 gcgcatatca gtaacatgaa acgttatcag tttaaggatg tcagtgatca ctgttgacgg
     1141 cagcctgtcc tttaccacat ggaatcgata cttgctgccg agaaaataga tggcatatgg
     1201 ctcaagccct ccgcagcgct cttttaattt gctatagtat tgagaggtct tgagtatcca
     1261 gttgcgcttt gaagcaacaa agctctcaag ctcttcggca ttgtagttca ggggtaccat
     1321 tgcccgcacg ccatttatgc cggatactaa tcgcaacctc tttgtcctct tgtttgggca
     1381 gatatcaatt ctaatggtcg tgttgtcagt aaaaacaagt gataaagaca attcacagat
     1441 ttagttttga taggtgttaa agaattttat gtaaaaaaac agatacactt aattgtactc
     1501 tttcttcata tgctgtacgg ctatagaata aatggatgca agctggcatg tatgcatcta
     1561 gttatacttg acattaacta gagaatatat gagtagagca cttagaaagg aggtgttggt
     1621 tcgttactag cacaatgttg gcacctttgt tgtgcagttc aaccactttc attattagga
     1681 attagaaaag caaataacaa atttatcaat tttgacatag agagatcgat taggattagt
     1741 caatagaaaa atctctgata aatttcacaa tatccttttt aataaaatga attcaaactg
     1801 tagacatcat ggtaacaggc aagtatttca ataaaggcgt caaatctttg gattcccctc
     1861 atgttgggca tgttgtaagg gaaacatcag acaagattgt agtctttggc gaaggcgatg
     1921 atagatatga tattccaaaa gacaagatta gatttgccgc agctaacgtt ctaattgact
     1981 taccattcta tgaaattgta aagaactaca aagttagcag agaggagcca ctacct
//
LOCUS       DASQTF010000002         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_279085,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000002 DASQTF010000000
VERSION     DASQTF010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279085"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..1353)
                     /locus_tag="VE308_00020"
     CDS             complement(355..1353)
                     /locus_tag="VE308_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HZA99386.1"
                     /translation="MFSQTHNMCQTAVFYGEPLARYGFGGSHPFGTDRLGAFWNKLQS
                     QSIGNIVVEEPVMSSYQDAVLFHDSAYVELVKVASRHGGVLLDKGDTPAFKGAFEASL
                     YVVGSTLAALDMVVTGIDSEKRKVDHAFNPIGGLHHARRDSAGGFCIFNDIGIAILAA
                     REKYRIKRIAYIDIDAHHGDGVFYEFEDDPLLFFADIHEDGRYLYPGTGSASEIGKGA
                     AASTKLNIPLEPGAGDDEFIEAFGKIEHFVDNVKPELIILQCGADGLAGDPITHLQYT
                     LKAHRFAADSIHRLAHKHCNGRIIALGGGGYNRANISAAWTEVIRSLADDIDFRQQ"
     gene            1407..2003
                     /locus_tag="VE308_00025"
     CDS             1407..2003
                     /locus_tag="VE308_00025"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZA99387.1"
                     /translation="MFTGIVKGVGMVTSVSKSKSGSEIIMRVRLGRFGQGLKKGDSVC
                     INGACLTATKLSKGDAEFEMVSETIRRTNLGRTKPGEMVNIERSLRVGDRLEGHFVLG
                     HVDGTGVVEDIQKMWSETTIWIKLDEWLTKSLVPKGSIAVDGVSLTLVGVEGDRVSVS
                     LIPHTLKVTTLGMKRRGDHVNIETDILGKYVMTNLPKV"
     gene            2054..2230
                     /locus_tag="VE308_00030"
     CDS             2054..2230
                     /locus_tag="VE308_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HZA99388.1"
                     /translation="MPIITVSTYSGRTQTEKDRLAEAITDDIIRIWKVQKKDVIVVFQ
                     EASHGNWYASGVRL"
BASE COUNT          654 a          516 c          498 g          605 t
ORIGIN      
        1 aactcctcag aacagatttt tagaacaacc gaaaaaggag acaagtttct aaaattatac
       61 agtcagatcg gagaaattgc accgccagct ttagacgaat ccatcccggc aagaagaaaa
      121 tagagacaca caaaaagaga agacggaagc tacgccttct aattcctttt acttcttgaa
      181 aatatatctc tgcaattgtc agaggtgaaa ccagcgttaa tggcacaaca acacctcatg
      241 tctagtttta tgtacaggct tcaatcaagg gataacgtat gactttctct atcatgttat
      301 catgatatta ctattttctt aatcttgatc ttttgatata gtttaagaag atcattactg
      361 ttgtctgaaa tcgatatcgt cagccagtga cctgatcact tcagtccaag cggcacttat
      421 gtttgcccta ttatagccac cacctccaag cgcaattatc ctgccattgc agtgcttgtg
      481 ggccaatcta tgaatagaat ctgcagcaaa cctgtgtgct tttaaagtat attgcaaatg
      541 cgttattgga tcacctgcaa gcccatctgc gccacattgt agtattatta attccggctt
      601 tacattatcg acaaagtgct ctatcttacc aaatgcttct atgaattcgt cgtctccagc
      661 acccggctca agaggtatgt taagcttggt gcttgccgct gcgcccttgc ctatctcact
      721 tgctgatcct gtgcctggat agaggtagcg gccatcttcg tgaatgtctg caaagaacag
      781 aagcggatcg tcttcaaatt cgtaaaagac tccgtctcca tggtgtgcgt caatatcaat
      841 gtacgctatc cgctttattc tatatttttc gcgtgctgcc aagattgcaa taccgatgtc
      901 gttgaatatg caaaaaccgc ctgcagagtc gcgtcttgca tgatgcaagc caccaatggg
      961 gttgaaggca tgatcgactt ttctcttctc gctgtctatg cccgtcacta ccatatcaag
     1021 cgccgccagt gtcgatccaa cgacatagag cgaggcttca aaagcgccct tgaacgctgg
     1081 tgtgtcaccc ttgtcgagga gcacaccgcc gtgccttgac gccactttta ccaattccac
     1141 atatgctgag tcatgaaata acaccgcatc ctggtagctt gacatcaccg gctcttctac
     1201 tacgatattg ccaatgcttt ggctctgcaa cttgttccaa aatgctccaa gcctgtcggt
     1261 tccaaatgga tgagagcccc caaacccata tcttgctagc ggctcaccat aaaacacagc
     1321 tgtctggcac atattgtgtg tctgagaaaa caatgaaagc aactgcaaat aaatattatc
     1381 aacaaccatt agagagctgc tactgcatgt tcactggaat tgtcaagggc gtaggcatgg
     1441 ttacgtccgt ctcaaagagc aagagtggat ctgagatcat catgcgagtc aggctcggaa
     1501 ggtttggaca aggtctcaag aaaggcgaca gcgtatgcat aaatggtgct tgcctcacgg
     1561 cgacaaagct atcaaagggc gacgctgagt ttgagatggt gtctgaaacc atccggcgaa
     1621 caaaccttgg ccgaaccaaa cccggcgaaa tggttaacat cgagaggtca ctgagagtag
     1681 gggataggct cgagggacac ttcgttttag gtcatgtgga tggcactggc gtcgttgagg
     1741 atatccaaaa gatgtggtct gaaacaacta tctggataaa gctagatgag tggttaacaa
     1801 aatcactagt gccaaagggc tcgattgcag ttgacggtgt tagcctcact ctggtaggcg
     1861 tcgaaggtga tcgtgtctct gtatcactca tacctcacac cttgaaggtg acgacgcttg
     1921 gcatgaagcg tagaggtgac catgtaaaca ttgagacaga tattcttggc aaatacgtca
     1981 tgaccaacct gccaaaagta tagaacttaa aataaaacat ttaatcttcc tatatgcatt
     2041 atttatcctg tatatgccta taataacagt atcaacatac agcggaagaa ctcagactga
     2101 aaaggatcga ttggcagaag cgataaccga tgacattata agaatatgga aggtgcagaa
     2161 aaaagatgtc attgtagttt ttcaggaagc atctcatggg aactggtatg cgtcaggcgt
     2221 tcgtttatag tgtcagagag atagttatta ctagcccaat tttattcacg acc
//
LOCUS       DASQTF010000003         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1204298,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000003 DASQTF010000000
VERSION     DASQTF010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204298"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VE308_00035"
     CDS             complement(<1..1171)
                     /locus_tag="VE308_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HZA99389.1"
                     /translation="MRENFFAEKTISVIKKAFREYYFNQSKAIEEPVKMEHREFGYMH
                     FGQAGMLRHLSFKSMKELDAMLVREAPSDVYCSNAYYRFPTQQPMQEKQWLGADLIFD
                     IDGKDLDMPCVPSHSYSVCINCGYASPLPEQDKGRSYSCPTCSFKKGDYVSIPCSKCI
                     DASKKEARRLMEFLLVDIGLEKSKIYTYFSGNNGFHVHLNDDACISLDPQARSDLVGY
                     LSGTGLMLESIGVRKTNTEDLFSIKFPKSGLAYGWRRRIAEKLKINGSTAIKLKNIVN
                     QSGGYNAFKVELDRLARHMGVRIDPQVTTDVHRVFRMPGTLNSKSGLSKAKSSDLPSF
                     DPFVDACLLGDSKVSVRVKTPVRLKLKRNSFNISKESAEMPTYAAVYLMCKGLAEA"
     gene            complement(1168..2181)
                     /locus_tag="VE308_00040"
     CDS             complement(1168..2181)
                     /locus_tag="VE308_00040"
                     /inference="COORDINATES: protein motif:HMM:NF016030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99390.1"
                     /translation="MSSIRMAGSDLAKYPFLNEAGEYLRESGFGWEELDRPDMKYIID
                     RAADRVEIEVDGKIYDNLERYEIEILTFLISLVIVKSIGIDQVLRKFALAEARRAEKF
                     LTEDLRNQSENKRRSVFTKIFEDLFKLKIVISEDQRLFKVRVVDYLARASHFHEQEWK
                     LINRLVHNGYVYLDADETVRLIRNELSVLIYDKVKAMTLPTLPLGIKSKADQLRLKLL
                     SRYERHAPTAITDYPPCIKHALEVMNKGDNLPHSARVMLATYMLAIDKPVDEIVLMFE
                     NAPDFNEKITRYQVEHLAGMKGSHTKYSVPSCNKLRNENLCFATAECDTLINPVQFGR
                     KKK"
     gene            2267..3130
                     /locus_tag="VE308_00045"
     CDS             2267..3130
                     /locus_tag="VE308_00045"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HZA99391.1"
                     /translation="MDIRVGFHVSIAGGISNSVNNAKKLGCTAFQIFSRNPRSWAAKP
                     LTLDEAESFRNRLCSSGIKKTSVVVHMPYLPNLSGPPGELYERSLKILIEEIHRCNLL
                     GISYLVVHLGSHMGRGSSNGIDQLVNALTAAAGAHSNSASAAVVLLENNAGQKNSIGG
                     TLEELRLILDRLDSSKQFGVCIDTCHLYASGYDLRTKEDVDKIFEKIKDILGLKELKI
                     IHLNDSKGELGSNLDRHEHIGLGSIGVEGITAFLNHRTIQGLPIIMETPIDKRRGDEQ
                     NLSVVLDMIKK"
     gene            complement(3149..3466)
                     /locus_tag="VE308_00050"
     CDS             complement(3149..3466)
                     /locus_tag="VE308_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99392.1"
                     /translation="MGYESDSSEFARISTKKLAILFGLAFFGAFMLIQMVQGFPLGEI
                     LFRQTVTQEVNVSIKQGNVCVIEPSDGQPKSIQGCPYNINDPLIVTYYEGSTNVESHR
                     LNK"
     gene            complement(3511..>3704)
                     /locus_tag="VE308_00055"
     CDS             complement(3511..>3704)
                     /locus_tag="VE308_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99393.1"
                     /translation="NLLKKHERNPFCYICNLFYTPEYLPVHLKADHTKEELAAHLLLA
                     YFDDWYEQEEAIEKATKTS"
BASE COUNT         1017 a          805 c          796 g         1086 t
ORIGIN      
        1 tggcttctgc aagccctttg cacataaggt aaaccgctgc atatgtgggc atctcagctg
       61 attcttttga aatgttgaat gagttcctct ttagttttaa cctgactgga gtcttaaccc
      121 tcacgcttac tttactatcg ccaaggagac atgcatcgac aaagggatca aaagaaggta
      181 ggtcgctgga ttttgccttt gacaaaccac tcttgctgtt gagcgttcca ggcatcctaa
      241 acaccctgtg aacgtcagtt gtcacttgtg gatctattcg gactcccatg tgccttgcta
      301 atctgtcaag ttcgactttg aatgcattat aaccgccgct ttggttcaca atattcttca
      361 gtttgattgc tgttgagcca ttgatcttga gcttctctgc aattcttcgt ctccacccgt
      421 atgcaagtcc gctttttgga aattttatgg aaaacaagtc ctccgtattg gtctttctta
      481 ctccaatact ttccaacatg aggcctgttc cagagagata ccccacgagg tcggagcgag
      541 cctgtggatc gagggagatg catgcatcat catttaagtg tacatgaaag ccgttgttac
      601 cggaaaagta agtataaatt ttacttttct ctaggccaat gtccactaac aaaaattcca
      661 tcaaacgtct agcctccttt tttgacgcat ctatgcattt gctgcaaggg atggaaacat
      721 agtcgccttt tttgaagcta caggttggac aagaatatga tctccctttg tcctgttcag
      781 gaagaggcga tgcatagcca caatttatgc agactgaata cgaatgcgat ggaacacacg
      841 gcatgtcaag atctttacca tcaatatcaa agatcaggtc tgccccaagc cactgcttct
      901 cctgcatagg ttgttgtgtt ggaaatcgat agtatgcatt tgagcagtag acatcagaag
      961 gcgcttccct gacaagcata gcatcaagct ctttcatgct tttgaatgat agatgcctaa
     1021 gcatccctgc ttgtccaaag tgcatataac caaactcccg gtgctccatt ttgacaggct
     1081 cttcaattgc ctttgattgg ttgaagtaat actcacggaa cgcttttttt atcaccgaaa
     1141 tagtcttttc agcaaagaaa ttctctctca tttctttttc ctgccaaatt gaactgggtt
     1201 tattagggtg tcgcattcag cagttgcaaa gcagagattt tcgttgcgga gtttattgca
     1261 tgaaggaaca gaatatttgg tgtggcttcc tttcatgccc gctaggtgct ccacttggta
     1321 tcgagttatc ttttcgttaa aatcaggagc gttttcaaac atcaagacga tttcatccac
     1381 tggcttgtct atagccaaca tgtatgtggc aagcatcact cgtgcggagt ggggtaggtt
     1441 atcgccttta ttcatcacct caagcgcatg cttgatgcat ggtgggtagt ctgttattgc
     1501 cgtcggggcg tgccgttcat aacgcgataa gagttttaac cgtagctggt cagcttttga
     1561 ctttatgcct agcggcagtg tgggaagagt cattgcttta actttgtcat aaatgagcac
     1621 ggagagctca tttctaatga gtctgacggt ctcatcagca tcgagataaa cataaccatt
     1681 gtggacaagt ctgttgatca gtttccattc ttgttcgtgg aaatgagaag ctcttgcaag
     1741 gtagtcaaca actcggacct tgaataaacg ctgatcctct gatataacga ttttcaattt
     1801 gaacagatcc tcaaagattt ttgtaaagac agaacgtctc ttattttcgc tctggtttct
     1861 taaatcctct gtgaggaact tctctgccct tcttgcttca gctagcgcga actttctgag
     1921 tacttgatcg atgccaattg acttgacgat cacaagagaa atgaggaatg taaggatctc
     1981 aatttcgtat ctctctaggt tgtcgtatat cttcccatcg acctctatct caacacggtc
     2041 ggcagcgcgg tcgataatat acttcatatc aggcctgtca agctcctccc agccaaaacc
     2101 cgactcgcgc aggtattcac cagcttcatt caagaacgga tacttggcta gatcgctacc
     2161 ggccattctt atagacgaca agtccgacaa taacatatag ctgctctacc ataaaaactc
     2221 cattacaata agttaaagta acaaaaatta tcttcatatc gaaacgatgg atataagggt
     2281 cggttttcac gtttccatag caggcgggat atcaaattcc gtcaataatg caaaaaaatt
     2341 aggatgtacc gcatttcaaa tatttagtcg aaatccgcga agctgggctg caaaacctct
     2401 aactctagat gaggccgagt ctttcagaaa taggctctgt tcaagtggaa ttaaaaaaac
     2461 atcagtagtt gtccacatgc catatctgcc aaacctctcg gggcctccag gagagttgta
     2521 cgaaagatca ctgaagattc ttatagaaga aattcataga tgtaatttgc ttggaatatc
     2581 ctatcttgtg gtacaccttg gaagtcacat gggcagggga tcaagtaatg gcattgatca
     2641 gctggtaaat gccttaacag ccgctgccgg tgcccatagc aactcagcaa gtgcagcggt
     2701 ggttctctta gaaaataatg caggacagaa gaatagtatc ggtggcacac tcgaagagct
     2761 gcgcctcata ctcgataggc tcgatagctc aaagcaattt ggtgtttgca tcgatacttg
     2821 ccatctttat gcctctggtt atgatctacg cacaaaggag gacgtagata aaatatttga
     2881 aaaaatcaag gatatcctcg gcctgaaaga attgaaaatc atccatctca acgattcaaa
     2941 aggcgagctg ggctcaaacc tagaccggca tgagcatatt ggccttggct caataggagt
     3001 tgagggtatt actgcatttc tcaaccaccg gacaattcag ggtctgccga taataatgga
     3061 gacgccaata gataagagga gaggagatga gcagaatctc agcgtcgttc tagatatgat
     3121 aaaaaaataa gatagtaaca ataataattt acttattgag cctgtgagat tccacattag
     3181 tagaaccttc gtaatatgtc acgattagcg ggtcgtttat gttataagga catccttgaa
     3241 tgcttttcgg ttgaccgtcg gatggctcta tgacgcagac attcccctgc tttattgaga
     3301 cattaacctc ttgagttaca gtctgcctga ataggatctc tccaagaggg aacccttgta
     3361 ccatctgtat gagcatgaat gctccaaaaa atgcaaggcc aaagagtata gcaagcttct
     3421 tagttgagat ccttgcaaac tctgatgagt cagactcata acccatatgc tactctgtgg
     3481 tgatcatttc taaaaaactt atgggagtga tcaggaggtc tttgtagctt tctcaattgc
     3541 ctcttcctgc tcgtaccaat catcgaagta tgctagcagg agatgagccg ctaattcttc
     3601 ctttgtgtga tctgctttga gatgaactgg cagatattcg ggtgtataga acaggttgca
     3661 gatatagcag aacgggtttc gttcatgctt ttttagcaag ttac
//
LOCUS       DASQTF010000004        10440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_741580,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000004 DASQTF010000000
VERSION     DASQTF010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10440
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741580"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VE308_00060"
     CDS             <1..225
                     /locus_tag="VE308_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99394.1"
                     /translation="QVKFLETEFEQNFLLYANDMVEFFEALAESGIISLAPDSQNQPS
                     SQNFFMIQLPKRDAAERALEIIKSNLKEHS"
     gene            248..430
                     /locus_tag="VE308_00065"
     CDS             248..430
                     /locus_tag="VE308_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99395.1"
                     /translation="MMMTHSRRKRPQEDQEVPLEIDTHYHLYGKHAWEVEYGERCPAC
                     NKRIDEFGFCACGSAQ"
     gene            610..831
                     /locus_tag="VE308_00070"
     CDS             610..831
                     /locus_tag="VE308_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99396.1"
                     /translation="MLDKWMEKMIESATSAYMPKVEEKAKARRAELEEVKTKVRTNPE
                     QVEEWFDKEIDKLQNIDIKEIMKNATAGI"
     gene            complement(813..887)
                     /locus_tag="VE308_00075"
     tRNA            complement(813..887)
                     /locus_tag="VE308_00075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(849..851),aa:Gly,seq:gcc)
     gene            complement(931..1551)
                     /locus_tag="VE308_00080"
     CDS             complement(931..1551)
                     /locus_tag="VE308_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99397.1"
                     /translation="MFATDSGSERATSIEDYQNTCTEFITDISRDYMDWIERYQLGEE
                     ETARRKATINYIVKTTNHWIAKYGTTIKKVDIVMNDGVNKMAENTAGYPFKFDVLVNT
                     MRRFTIHPVGEVRVNIKATPRPGRLARIGNYQKDQLLLINSSSPVRFSISKESMKAAD
                     ILDDYIIIDANNCHSHDLISLTPVVEEVEGEYVTESRGHTTIILLQ"
     gene            complement(1603..2610)
                     /gene="hemB"
                     /locus_tag="VE308_00085"
     CDS             complement(1603..2610)
                     /gene="hemB"
                     /locus_tag="VE308_00085"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZA99398.1"
                     /translation="MKRTNINSGINSFPNVRLRRLRTTPAVRDLLQETRLSPKDLIAP
                     IFVQEGLNKTQEIPSMPDIHRMPISKLAGEVDRLMELGISAIILFGLPLQKDVNAKSA
                     FDDSGIVQKSIELLRNHFGTKVAIITDVCLCQYTSHGHCGIVQNKKIDNDKTIHALAK
                     VAVSHASAGADIVAPSAMMDGQVQALRTGLDGAGFPDVAIMGYSAKQASPLYAPFRNA
                     AHSAPEFGDRRTYQMPYSNAREAMREIELDIAEGVDIVMIKPAIPYLDLVYTARQSTH
                     VPICAYSVSGEYALIKAAAMNGWIDEDSVMTEFLTSIKRAGANMIITYQAKRMAQLLS
                     K"
     gene            complement(2579..3919)
                     /gene="hemA"
                     /locus_tag="VE308_00090"
     CDS             complement(2579..3919)
                     /gene="hemA"
                     /locus_tag="VE308_00090"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZA99399.1"
                     /translation="MGEKRQNMTAIVKATPETRVGLARIVNARVTYRNAPMHLLEKFT
                     FKDIDKAHKVFLNRAGFNECVILQTCNRVEIFGAAKDPDEDKLLEEWVSAAGLSEKDL
                     SSIEINRGKDTIVHLMRLTSGLESLVVGEDQILGQVRRAFEFSRAHGYASANLSVIFD
                     RALKAGSRIRTATGINKGNVSIASVAVNLAEEYFEDLKKKSVLLIGTGEAASLVAKVL
                     KRRDINFMIASRVPERAHAFAETVAGVPIAFETALEMLRIVDVIFTATMAPYHLITYE
                     RIQNAMMNRKGRRSMMIFDLSNPRTVDERVATIRGVKLINMDQIAELVGKNMRSRMKE
                     VDSAEKMINEEMKSFDTIMKRMKAEPVVMAVFRNVDTIRERELKKALTMIGKKLAPEE
                     AKIVEQLSYAIVEGILSIPMNNLRKEIEEGGNEELMKIVTKLFMYEENQHQQRH"
     gene            complement(3895..4566)
                     /locus_tag="VE308_00095"
     CDS             complement(3895..4566)
                     /locus_tag="VE308_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HZA99400.1"
                     /translation="MIVDLNIKGKQAIVIGGGTEGIRKIRGLLGQDCKITVISNRVNR
                     FLVEQADLGKIKLVKAKLRDANILDNYNDAFLVLAATNDRELNRKIVERGRTIKAFVY
                     AADDPAVSDFSYASIVNIEGIVQVAISTSGKSPLMARKIRIKAERVLRRIIKKADIEN
                     AKLQEFARQAARPHINTVDERKEFLYSVIKNREIQNLIKEDRIEDAKSATLELLHKWE
                     KKDRT"
     gene            complement(4643..5353)
                     /locus_tag="VE308_00100"
     CDS             complement(4643..5353)
                     /locus_tag="VE308_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HZA99401.1"
                     /translation="MNNRLEESQADEKGQEETEFGDDIKEDSNFAGPKITSEQDNITS
                     SREYQIEKPTPEIREEEMASTVETVASSPSSSAATRQPNQAVQSSRESKFFAIRTTGG
                     QERVVANMVQTRANAKKLLIRSIIVLDSFKGYIIVEAPDSNVAYEALAGIRHVRGQIR
                     GDLPFKDIEGYLVKKPVVSELSTNDTVEVIAGPFKSMKAKITRVDYEKQEATVVLLDS
                     PYQIPVTVDANYLKRLAH"
     gene            5752..6582
                     /locus_tag="VE308_00105"
     CDS             5752..6582
                     /locus_tag="VE308_00105"
                     /inference="COORDINATES: protein motif:HMM:NF016312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZA99402.1"
                     /translation="MNEQYVFVASGHDLAGMTMSAYLMSSAEFASESNRNADGQSYRS
                     PRYKNIKLYIFSGNLLTLENIPDLYSHSDVFIFLSKHRSQSSVPTLTCHFTGNFSADA
                     SYGGNPRQIAISYPSLQKGYLKAITAARQKVPGYEVIIEATHHGPTSLNKPVLFIELG
                     SSEKQWADENAAAVICDTLLDVLDNGFERYDKVGIALGGTHYPKKFNKLLLESKFALA
                     AVASKHNLKAIDQEMLDQMIEKSIEKVTHVVLDSKGLGRQKDGILRMLEKTPLELYKV
                     "
     gene            6622..6999
                     /locus_tag="VE308_00110"
     CDS             6622..6999
                     /locus_tag="VE308_00110"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZA99403.1"
                     /translation="MKPRILNASDVYDIVAQIPVGKVTTYGDIARALGHPRASRLIGR
                     ILNKNPNPIVTPCHRVIKSDGSIGGYAFGKVRKKDLLKKEGLCFIGDSTAEFEKYRIP
                     LQKLCYEDLASFTSSRKCESDLT"
     gene            complement(6942..7388)
                     /locus_tag="VE308_00115"
     CDS             complement(6942..7388)
                     /locus_tag="VE308_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HZA99404.1"
                     /translation="MVVNIAKKRELVARILRVGANRIRFEPDRLEDIADSITRDNIRS
                     LVKSGAIWTVQPKGSSRGRAIKKRSVWKVHGKGPGSKKGKKTARTGKKEVYVVRIRSM
                     RYHLKVIKERKDITNDVYWHLYKKVNGGQVRSLSHLRELVKEAKSS"
     gene            complement(7390..7812)
                     /locus_tag="VE308_00120"
     CDS             complement(7390..7812)
                     /locus_tag="VE308_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HZA99405.1"
                     /translation="MVINEELLAARKKVAQRRPKFIRQESWRYDRLAENWRKPKGKDN
                     KMRLQKSGMPALVKVGYRGPRAARGLHPSGYRDIIIHNTAELVRLDPKKDAARIGHTV
                     GKKKRIEIINKAVELDIKVLNPGNLISKMQKEKEKKKE"
     gene            8329..9756
                     /gene="gvpD"
                     /locus_tag="VE308_00125"
     CDS             8329..9756
                     /gene="gvpD"
                     /locus_tag="VE308_00125"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD P-loop
                     domain-containing protein"
                     /protein_id="HZA99406.1"
                     /translation="MAEDDVSFKDKNGSSPSHIPEELLHFIQRDTYSLLVKGFAGTGK
                     TTLALTILNVLGIKNNFFYISTRLSPKQLFQYYPWLKDFIEQSKPRGTSLDRDHRVMS
                     SFEDARLDEPESLFERITNQLMDIKSPIIIIDSWDAIASFMDREARLNNERVLQTWRE
                     RAGAKLIFISEQPADTTLDFLVDGIVELKQIYYNNVKIRQIILQKLRGTRINRPSYLY
                     TLENSIFHSFVPYHLVNFEPRKRGATNVWERSTGDNIQSGYPMLDASLGSGFPRKGVV
                     LLELDSHVNMTIAMLFLQKIVSNFVLSGNPVLLQPVNWMEQSSVLRFFGTSIPAGKKS
                     LLKILWTGKSNRISNNIISTGKNQSSDRLLATLVKMKQRHRDRVLLNIMWIDAIQSFY
                     EARNARSGIKNMLSRIMTHADLSIVILPHSRGDMLELVSEVSNIHLRFLMINDTLFLQ
                     TLVPSSNLYSIVFDRHSEMSLKPVV"
     gene            complement(9765..10061)
                     /locus_tag="VE308_00130"
     CDS             complement(9765..10061)
                     /locus_tag="VE308_00130"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZA99407.1"
                     /translation="MKNRSRYEVIAAILKSVNKEETRTKIMYKAMLSNDQCKLYLDSL
                     IKSGLVHEVSNGEKILYRITPKGSRFLSYYDQMKELLPVGIEENLADEYYHLST"
     gene            complement(10185..>10440)
                     /locus_tag="VE308_00135"
     CDS             complement(10185..>10440)
                     /locus_tag="VE308_00135"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZA99408.1"
                     /translation="IEFILLMCENGARKTHVMYRCNLNSKQINQYLQFLLDSGMLETI
                     QERPNSKRYIYKTTDLGKKFIAHYKQLAGLFTKVPPPSLT"
BASE COUNT         2728 a         2392 c         2212 g         3108 t
ORIGIN      
        1 caggtaaagt tcctcgagac tgaattcgaa cagaattttt tgctatacgc aaatgacatg
       61 gtcgagttct ttgaagcgct agcagaaagc ggaataattt cactagctcc agactcacaa
      121 aaccagccca gttcacaaaa cttcttcatg atccagttac caaaaaggga tgctgcagag
      181 cgcgcacttg aaataatcaa atccaacttg aaagagcaca gttaattatg tctatcagca
      241 atggaagatg atgatgacac attcgcgtcg taaaaggcct caggaagacc aagaagttcc
      301 tcttgagata gatacccatt atcatctgta tggtaagcat gcctgggaag tagaatatgg
      361 cgagcggtgc cctgcttgca acaagcggat agacgagttt ggcttttgcg cgtgtgggag
      421 tgcccagtaa tccttgaagg tatgatgggc agaattaccg tctagagcat tattgtattg
      481 cccttataag aaatgttaaa caacaacgaa tgacatgact tcaacgatat ggactattat
      541 cggtgttgtg atcctcataa tagtgataat aattctgcta agattacttg ggatctgggt
      601 ctgatttaga tgctggacaa atggatggag aaaatgatcg aaagcgcgac atctgcctat
      661 atgcctaagg tggaagagaa agccaaggcg aggagagcag agttagagga agtgaagacg
      721 aaggtgagaa ctaatcctga gcaggtagaa gagtggtttg acaaggagat agataagctt
      781 cagaatatag acataaaaga aattatgaaa aatgcgacgg ccgggatttg aacccgggtt
      841 accagattgg caatctgatg tcctagacca aactggacga ccgtcgctcg gaaactatcg
      901 acactacaag gctatttaaa gcgtacgagt ctactgaagc agtattattg tagtgtgacc
      961 tctactctcg gtgacatatt caccctcgac ctcctccacc acgggtgtta aagaaatcaa
     1021 atcatggcta tggcagttgt tggcatcgat tatgatgtag tcgtctagaa tgtcggcagc
     1081 tttcatgctc tctttgctta tagaaaatct gactggagag ctggaattta tgagcaacaa
     1141 ttgatctttc tggtagttgc caatccgcgc gagccttcca ggtcttggcg ttgccttgat
     1201 gttgacccgt acttcgccta cagggtgaat tgtaaagcgc ctcatcgtgt ttacgaggac
     1261 atcgaacttg aagggatacc ctgcagtgtt ctctgccatc ttgttaacac catcattcat
     1321 tacaatgtca acctttttga tagtagtgcc atattttgca atccagtggt tggtggtctt
     1381 gacgatgtag tttattgttg cctttcttct tgcagtttcc tcctcgccca actggtatcg
     1441 ctcaatccag tccatatagt cacgactaat gtctgtaatg aactcggtgc atgtgttttg
     1501 gtagtcctct atgctagtag cccgttcaga gcctgaatca gtcgcaaaca tattggatag
     1561 gattcatgcc gcatgccgat ttaaaaggat gaatttatac ggctacttac tgagtagttg
     1621 tgccatcctt tttgcttggt acgtaattat catattggca ccagctcgtt ttatagatgt
     1681 taggaactcg gtcatgaccg agtcttcatc gatccagccg ttcatagcag ccgccttgat
     1741 cagcgcatac tctccagata cactgtaagc acatatcggg acgtgggtgc tctgcctggc
     1801 agtgtataca agatcgagat aaggtatcgc cggcttaatc atgacaatgt caactccctc
     1861 tgcaatatct aactctattt cacgcatagc ttcccttgca tttgaatacg gcatctggta
     1921 cgtgcgtcta tctccaaatt cgggagcaga atgagctgcg ttgcgaaatg gagcgtaaag
     1981 cggggatgcc tgcttagcag agtatcccat tattgccacg tctggaaatc ccgcaccgtc
     2041 taggccagtc cgcaatgctt gcacctgacc atccatcatt gcagaaggtg ctacaatgtc
     2101 tgcaccggcg cttgcatggc tgacagccac ctttgccaaa gcgtgaatgg ttttgtcatt
     2161 gtctatcttt ttgttttgta caatgccgca gtgcccgtga gaagtatact ggcacagaca
     2221 aacatcagtg attattgcca ctttggtgcc aaagtgatta cgcaagagtt caattgactt
     2281 ctgtacgatg ccgctgtcgt caaaggctga ttttgcattt acgtccttct gcaaaggcaa
     2341 accaaatagg atgattgcgc tgataccaag ctccattaag cgatcgacct caccagcaag
     2401 tttggagatc ggcatgcggt ggatgtctgg catagagggt atttcttgtg ttttgtttaa
     2461 cccttcctga acaaatatcg gggctattag atctttaggc gaaaggcgtg tctcctgcag
     2521 cagatccctc actgcagggg ttgtcctcag acgccgaagt cttacatttg gaaaagagtt
     2581 aatgccgctg ttgatgttgg ttctcttcat acatgaacaa cttggtcact attttcatca
     2641 gctcttcatt accgccttcc tctatctctt ttcgcagatt attcatggga attgaaagga
     2701 tgccctcaac aatcgcgtag gaaagctgct ctacgatttt ggcttcctcc ggagccagct
     2761 tttttccaat catagttaac gcctttttca gttcccgctc tcttatcgta tcaacatttc
     2821 tgaacactgc catcacaacg ggctcagctt tcattctctt catgattgtg tcaaatgact
     2881 tcatttcctc atttatcatc ttctctgcag aatcaacctc cttcatgcgg gagcgcatgt
     2941 tcttgccgac gagctcagcg atctggtcca tgtttataag tttgacgcca cggatagttg
     3001 ccactctttc gtcgactgtc cttggattgg acagatcaaa gatcatcatg ctcctcctgc
     3061 cctttctatt catcattgca ttttgtatcc tttcgtaagt gattaagtgg tagggagcca
     3121 tagttgccgt aaatatcacg tcgactatgc gtagcatttc tagagcggtt tcaaaggcaa
     3181 tcggaacccc tgcgacagtt tccgcaaatg catgtgctct ttcaggaacc ctgctagcta
     3241 tcataaagtt gatatcacgc cttttgagca cctttgctac aaggcttgcc gcctctccgg
     3301 tacctatcaa caggacactc tttttcttta agtcttcaaa gtattcttcg gcaaggttca
     3361 cagctactga cgcaattgaa acattgccct tgtttatgcc agtagcagtc ctaatcctgc
     3421 ttccagcctt tagagctcta tcaaaaataa cggataggtt tgcgcttgca tacccatgag
     3481 cacgtgaaaa ctcaaaggct cgcctcactt ggcccaaaat ttggtcttcg cccacaacca
     3541 gcgactcaag gcctgaagta agcctcatca gatgcacaat tgtgtccttg cctctattga
     3601 tttctatgct gcttaggtct ttttctgaca gcccggcggc agaaacccac tcctcaagta
     3661 gcttgtcctc atcggggtct tttgctgcac caaaaatttc aaccctattg catgtctgca
     3721 aaatgacaca ttcgttaaag cctgctctgt taagaaagac cttgtgtgcc ttatcgatgt
     3781 ccttgaatgt gaacttttcc agcagatgca tgggcgcgtt gcgataggta acccttgcgt
     3841 tgacgatccg agcgagacca actcgtgttt ctggcgtggc tttgacaatt gctgtcatgt
     3901 tctgtctttt ttctcccatt tatgtaacag ctctaacgtc gcgcttttag catcttcaat
     3961 cctgtcttcc ttaataaggt tctgaatttc tctgtttttt atcacggaat aaaggaactc
     4021 tttacgctca tctaccgtat taatgtgagg tcgagctgct tgccgagcaa attcctgaag
     4081 cttggcgttt tcaatatccg ccttcttgat tatcctgcgc aggacccgct ctgcctttat
     4141 ccttattttg cgtgccatta gagggctttt accagaagtt gaaatagcta cctggactat
     4201 cccctcaatg ttaacaatgg aggcatagga aaagtcgctc accgccggat catctgcagc
     4261 ataaacaaat gcctttatcg ttcgccccct ttctacgatc ttgcgattta gttctctatc
     4321 attggtcgcg gctaggacca aaaatgcatc attgtaattg tcaagtatgt tggcatcgcg
     4381 aagctttgcc ttaacgagct tgatcttgcc tagatcagcc tgctcaacca gaaagcggtt
     4441 aaccctgtta cttattaccg ttatcttgca gtcctggccc aagagtccgc ggatttttct
     4501 aataccctca gttccacccc ctataactat tgcctgtttg cccttgatgt tcaggtcgac
     4561 aatcaactat aacaccagaa agttaaacaa taaatttggc tacatatctt tatttaatcg
     4621 ttcagttcta tgcaacaatt gcctagtggg ctaacctttt gaggtagttt gcgtcaacgg
     4681 ttaccggtat ctgatatggt gaatcaagca gaacaactgt tgcctcttgc ttttcataat
     4741 ctacgcgagt tatcttagcc ttcatggatt tgaatgggcc cgcaataacc tcaactgtgt
     4801 catttgtact aagctctgac actacaggct tcttcaccaa gtacccttcg atgtccttga
     4861 aaggcaaatc gccccttatc tggccgcgaa catgtcttat tcctgcaagt gcctcatatg
     4921 cgacgttgga atccggcgcc tccacaatga tgtaaccctt aaaactatca agtacaatga
     4981 tggagcgaat taacaatttc ttggcgtttg cccttgtctg taccatgttt gcaactacgc
     5041 gctcctgacc tcctgttgtg cggatggcaa agaacttgga ctcccgtgag gattgtacag
     5101 cttggttcgg ttgtcttgtt gctgcagagg aagaaggtga acttgcaact gtctcgacag
     5161 ttgaggccat ctcttcctct cttatctccg gcgtcggctt ctcgatttga tactctctgg
     5221 agcttgtaat attatcctgt tcggaggtta tttttggtcc tgcaaaatta gaatcctctt
     5281 taatatcatc tccaaattca gtttcctcct gacccttctc atctgcttgg gactcttcta
     5341 atctattgtt catcttgttt gctatgcaaa ggtctgacgc atttaataaa tttactcagg
     5401 cagaacgatg atgaaaatgt aattcttaca caaaggatac agagtcttct tatagatatt
     5461 gcatagataa tactacgagt gctggctgtt cttctgggat tacgtcatac ttggcaattc
     5521 tcctgtctaa accctaggaa gggtgttact ttagactagc atctttctaa ctcttcttgt
     5581 cacttattgt taagaaagga tgactcctat cctcttgaaa tttatgatgt tcttggattg
     5641 gaatgcggta gtcgccttca tagctgcaag atggtaccat ctgttccttt gcttaattta
     5701 cccttgcgat ctgacaaggc taaaattagt actgtctgta gacgctctta gttgaacgaa
     5761 caatacgtct ttgtagcgtc tggtcatgac ttggcaggca tgacgatgag cgcctatctg
     5821 atgagtagcg ctgaatttgc tagtgagagt aatagaaacg ccgacggcca gtcatacagg
     5881 tctccccgct ataagaatat caagctctat attttctctg gcaatttgct cacgttagaa
     5941 aacattcctg atctctattc gcactcagat gtgttcattt tcctttcaaa gcataggtcc
     6001 caaagctccg ttccaacact gacatgccac ttcaccggca atttttctgc agacgcctct
     6061 tacggtggta accctcgaca gatagcaatc tcgtacccga gtctgcagaa aggctatttg
     6121 aaggccataa ctgctgcgag acaaaaggtt ccaggatatg aggtgatcat agaagctaca
     6181 catcatggac cgacatccct caataagcct gtattattta tagagttggg atcttcagaa
     6241 aagcaatggg cagacgaaaa cgccgcggca gtgatatgtg acaccctgct tgatgtactt
     6301 gacaatgggt ttgagcgtta cgacaaagta ggcatagcgc ttgggggcac acattatcca
     6361 aaaaagttca acaaactctt gcttgagtca aaatttgctc tcgcggcagt ggcatcaaag
     6421 cacaatctca aagcaattga tcaggaaatg ttagaccaga tgatcgagaa gagcatagag
     6481 aaggtgacac atgtggtatt ggatagcaaa ggcttgggaa gacaaaaaga tgggatactc
     6541 agaatgcttg aaaagactcc tcttgaactt tacaaagtct gaatgcggta ttttaaatat
     6601 ttgcatacca tcagatatgt tatgaaaccg cggatactaa atgcaagcga tgtttatgat
     6661 atagtcgctc aaattcctgt aggtaaagtc acaacttatg gagatattgc cagagctctt
     6721 ggacatcccc gtgcatcccg cctgattgga aggatattga ataaaaaccc aaacccgatt
     6781 gtgacgccat gtcatagagt gatcaagtcc gatggcagta taggtggata tgcttttggc
     6841 aaggtaagga aaaaagattt gctaaagaaa gagggtctgt gctttattgg cgacagtaca
     6901 gcagaatttg agaaatacag aattccgctt cagaaattgt gctatgagga cttggcctct
     6961 tttacgagct cccgcaaatg cgaaagtgat ctcacctgac caccattgac cttcttgtag
     7021 aggtgccagt acacatcatt ggtgatgtct tttctttcct tgataacttt tagatggtaa
     7081 cgcatcgacc ttatcctcac cacatagacc tctttcttgc ccgtccttgc agtcttttta
     7141 cctttctttg agcccgggcc cttgccatga accttccaga cggagcgttt cttaattgct
     7201 ctacctcttg aacttccctt tggctggact gtccaaatcg cgccgctctt gacaagcgag
     7261 cgtatattgt ctctagtgat ggagtcggcg atgtcttcaa gtctatccgg ttcgaagcga
     7321 attctgttgg ctcccaccct gagaatcctt gctacaagct cgcgcttttt tgcaatgttg
     7381 actaccatgt tactcctttt tcttctcctt ttctttttgc attttagata ttaggttacc
     7441 aggattgagc acttttatgt caagttcaac agccttgtta attatctcaa ttctcttttt
     7501 cttgccgacg gtgtgaccaa ttcttgccgc gtccttttta ggatcaagcc taaccagctc
     7561 ggcggtgtta tgaataatga tatcacgata tcctgacgga tgaagacctc tagctgctct
     7621 cggaccccta tagccgacct tgacaagggc gggcattcct gacttttgca acctcatctt
     7681 attgtccttg cctttaggtt tcctccagtt ctcggcaagc ctatcatatc tccagctctc
     7741 ttgcctgatg aacttgggtc ttctttgtgc aaccttcttc ctggctgcta gtaattcctc
     7801 gttaatcacc atagttttac ttagaagact cctatccgat tacaataaat atgttactcg
     7861 tttcaaactt ttcgataaac agaaaagaag tataaatgat gatgctttat atttctctcc
     7921 atcaaaaacc agagacgttg tgtctttctc tagcatcttt tgcaaacccc ttatattttg
     7981 cacaagttct aacgcctgtg cataagatag ggcggatggt tacgcgttga ggagatgatg
     8041 agagtgtgct actggcattt gcctaacttt ctggaatcaa atatagcgcc gtcttctacg
     8101 gcaacatata gagtgctttc tctgggatag ttattatcgg acaccagctc gacctcatag
     8161 ctcacgccat attgtttgag gccgtatttc atgatgaact atattctata ttgttatggg
     8221 atttgggtta tggcaaagat atgctcaatc cttaatagga tagctaaatc actaacaatg
     8281 aattcgtatc aaagggactt gaaagaatct ggttgtggtg aataatactt ggcagaggat
     8341 gatgtgagct ttaaggataa aaatggaagt agcccatcgc acataccaga ggagcttttg
     8401 cactttatac aacgtgacac ttactcgctt ctagtaaagg gatttgctgg aaccggcaag
     8461 accacgcttg cacttacgat cttaaatgta ctcggaatca agaacaattt cttctacatc
     8521 tcgacaagat tatcaccaaa gcagctcttc caatattacc catggctgaa ggattttatt
     8581 gagcaatcta agcctcgggg cacaagccta gatcgagatc atagagtgat gtcaagtttt
     8641 gaggatgcaa ggctagacga acccgagtcg ctctttgagc gcattacaaa ccagctaatg
     8701 gacatcaagt ctccaataat cataatcgac agttgggacg caattgcttc tttcatggac
     8761 agagaggcac gccttaacaa tgaaagggtg cttcagacat ggagggaaag ggctggtgct
     8821 aagctcatat tcataagcga gcagcctgct gataccacgc ttgactttct ggtggacggg
     8881 atagttgagc tgaagcaaat ctactataac aatgtaaaaa taaggcaaat aattcttcag
     8941 aagctgcggg gcacccgtat aaaccgccca tcttacctct atacactcga aaacagtata
     9001 tttcatagtt ttgtgcccta ccacctagtc aattttgaac cacgcaaaag aggtgcaaca
     9061 aacgtctggg agcgttcaac gggcgataat atccaaagcg gctaccctat gctggatgct
     9121 tcacttggca gcggctttcc aaggaaaggt gtggttcttc ttgagctgga ctcacacgta
     9181 aacatgacaa ttgcaatgct atttcttcaa aagattgtat caaattttgt tttgagtgga
     9241 aacccggtgc tgttacagcc tgttaactgg atggagcagt cttcagtatt acgcttcttc
     9301 gggacctcca ttcctgccgg caaaaagagc cttttgaaga tattgtggac aggcaaatcc
     9361 aacagaatat ccaataacat catatcgaca gggaaaaatc agagctccga tcggctgctt
     9421 gcaactcttg tcaagatgaa acaaagacac cgcgacagag ttcttcttaa tataatgtgg
     9481 atagatgcta tacaaagttt ctatgaagca agaaacgcga gaagcggaat aaagaacatg
     9541 ctatcacgca taatgaccca tgcggacctt tcaatagtta tactccccca ctcacggggc
     9601 gatatgctcg agcttgtttc agaagtttca aacattcacc tgcggttctt gatgataaat
     9661 gacacactgt ttctgcagac gcttgtccca tcatccaatt tgtattctat tgtatttgat
     9721 aggcattcag agatgagctt aaagccagtc gtatgatcat gtgatcaagt gctcaggtgg
     9781 tagtactcat ctgctaaatt ctcttcaatc cccactggga ggagttcctt catttggtca
     9841 taataggaca ggaacctgct tccctttggt gttatcctat acagtatctt ttccccatta
     9901 ctgacctcat gaacaagccc gcttttgata agcgaatcca gatatagctt gcactggtca
     9961 ttactcagca ttgctttata cataatcttg gtgcgcgtct cttccttgtt tacagacttc
    10021 aagatggcgg cgatgacttc gtagcgactg cgattcttca tttaagcgga ctgaactaca
    10081 cctacatttc atataatccc tactactatt tctcatagaa gtatatgaca caataatatt
    10141 tcatttaatt gttcacaatt gtcacaagta tctgcattat tcatttatgt aagcgaaggt
    10201 ggagggacct tagtaaagag ccctgccagt tgcttataat gcgcaatgaa ctttttacca
    10261 agatcggtcg tcttgtatat gtagcgcttt gaattcggcc gctcctgtat cgtctcaagc
    10321 attccgctgt cgagcaggaa ttgaagatac tggtttattt gcttggagtt cagattgcat
    10381 cgatacatta catgggtctt tctagcccca ttctcacaca tgagcagtat gaattctatt
//
LOCUS       DASQTF010000005         2331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_279625,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000005 DASQTF010000000
VERSION     DASQTF010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279625"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VE308_00140"
     CDS             complement(<1..277)
                     /locus_tag="VE308_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZA99409.1"
                     /translation="MNVLDFGIEVIALSASGVLAPGPLFFANLFYGARHDKWSGIKVA
                     YGHTVVELPLIIILAAGLFTFDAAKKNAGTLGLIGGIAILAFAALQIA"
     gene            367..1440
                     /locus_tag="VE308_00145"
     CDS             367..1440
                     /locus_tag="VE308_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZA99410.1"
                     /translation="MIDTIRTIRLISKWGTNMALGRRRPVVAVFSMTHYCNFYCPMCP
                     FGNPDKEGQIKLAKTSDLTTEQWKTVFDKVSKYCIWCIIEGGEPTSRPDFMELVEYLH
                     SIKIPITLITNCSLLHTIDLENLRKYIQFVTCSIDSTFEESYCKVRGVSPHIFRRVMD
                     NLRLLTKHEVPHYFNSVITKFNTQEFIDQSYFDRAKDLGASAISLTFVEDRSDVDYSL
                     LPDRETMVKVCTSILNYMKKSSSLKMLIPPEYFQQIIEHGRTVFDECGVWKSIFVNGN
                     GTVMVPCWKFNSPENTYSLLEKSIDEIWNAPEWNIARKCHDCKVLGCIWTSSQPIRTL
                     ARSYMRGLPKLISQYRPDLEELT"
     gene            2053..>2331
                     /locus_tag="VE308_00150"
     CDS             2053..>2331
                     /locus_tag="VE308_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99411.1"
                     /translation="MLTDDDEGIRVPLQHPACQVAACFERASVYVVIKESDINALNVD
                     TGENQMVMERDSLADVNAFKLILYLCARHDYDLRYLLEQNELEITKRHH"
BASE COUNT          719 a          472 c          515 g          625 t
ORIGIN      
        1 ttgctatttg caaggcagca aaggcaagga tagcgattcc cccaatcaac cctagggtgc
       61 cagcgttttt ttttgcagcg tcaaatgtaa acaggcctgc tgctagtatg attattagag
      121 gtagctccac tacggtgtga ccgtatgcaa ctttgatacc agaccacttg tcgtgacgtg
      181 caccgtagaa cagattggca aaaaaaagcg ggccaggtgc cagtacacct gatgctgaaa
      241 gagctataac ctctatgcca aagtccaaca cattcattgt ccttgtgata acgttgtcac
      301 tgtactgtta aggttttcta tacaagagaa ttgcttaaaa gtaacaactg atgcaaaagt
      361 catacattga tcgacacgat acgcactatc aggctcatca gcaaatgggg tacaaatatg
      421 gcattaggga ggaggcgtcc tgttgtggca gtgttcagca tgactcacta ctgcaatttt
      481 tactgtccga tgtgtccctt cggcaatccc gacaaggaag ggcaaataaa gctggcgaag
      541 acttccgatc ttacgacaga gcaatggaag acggtctttg acaaggtgtc gaaatattgt
      601 atctggtgca taatagaagg cggagagccc acgagcagac ctgactttat ggagcttgtt
      661 gagtatctgc atagcatcaa gattccgata acgcttatca ccaactgctc gctcctgcac
      721 accatcgacc tagagaatct gagaaaatat attcagtttg tgacatgcag catcgattcc
      781 accttcgaag agtcatattg taaagtgcga ggagtgtctc cacacatctt ccggagagtg
      841 atggacaacc tacgcctact gacaaaacat gaggtacccc actactttaa cagtgtgata
      901 acaaaattca atacacaaga gttcatagat cagagttact ttgacagggc aaaagatctc
      961 ggagcaagcg caatctctct tacatttgtt gaagacaggt ctgatgttga ctattcccta
     1021 ttgccggatc gggaaacgat ggtcaaggtc tgcacaagca ttctcaatta tatgaaaaag
     1081 tctagctcac ttaagatgct aataccacca gaatattttc aacagataat agagcatggg
     1141 aggacagtct ttgacgaatg cggagtttgg aaaagtatat ttgtaaacgg aaatggaact
     1201 gtcatggtac catgttggaa atttaactct ccggagaaca catatagcct tcttgagaag
     1261 agcatagatg aaatttggaa cgctcctgag tggaacattg ccaggaaatg ccacgattgc
     1321 aaggtgctcg gttgcatctg gacttcatca caaccaatcc ggactcttgc tagaagctat
     1381 atgcgggggt tgccgaagtt aattagccaa tataggccag atcttgaaga gctgacgtag
     1441 aaacaaataa catgtggaag catccgaatc attttctata gttatgtatc aaagtctaag
     1501 catagctaca ttactaccta tggtgatcct gatagagcga acatgatgtg ttcacaagag
     1561 gcatgatctt tcaaacagat gacatactgc ctgatgtgtg tatattattt tgacacacgg
     1621 atctgaaaat actccatcag tttaaagaaa gctctgtgct atcaggtaac gttagaagtt
     1681 gcaatcagca cattctctag gaatgctcta ctgcaagcag gactctctgg gaccttggga
     1741 agtatggcga agaatgttac ggctgccgat ggcggatacc gtgcgcacga caaaatatgg
     1801 catatcgttc gaacttcggt agccaagtct ttaagatcgg gttaattata gatagagttt
     1861 atacatctag gtaaccttag tatgacaatt ttcgatccag tacttacttt taatagcaat
     1921 tgttgtatgt taatattaag cagttctata gactatatag taaaataaat ctattttttc
     1981 attactaata acaattcaat agagacagaa ataacctgta aatatcaaac tttgacatat
     2041 tgtattgcaa atatgctaac cgatgatgac gaaggcatta gagtgccatt gcagcatcca
     2101 gcatgtcaag ttgctgcttg ctttgagcgt gcatccgtct acgtcgtcat caaggaaagt
     2161 gatatcaatg ccttgaacgt tgataccggc gaaaatcaga tggtgatgga gagggactcg
     2221 cttgcagatg tcaacgcttt caagctcata ttgtaccttt gtgcacgcca tgactatgat
     2281 ctcaggtatc tgcttgagca aaatgaactt gagataacaa aaaggcatca c
//
LOCUS       DASQTF010000006         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_464596,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000006 DASQTF010000000
VERSION     DASQTF010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464596"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1121
                     /gene="rrp4"
                     /locus_tag="VE308_00155"
     CDS             9..1121
                     /gene="rrp4"
                     /locus_tag="VE308_00155"
                     /inference="COORDINATES: protein motif:HMM:NF003181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HZA99412.1"
                     /translation="MQELKRKYVIPGDKIVDGNFRPSMNVIRVGNSIFSTRIGIAETG
                     RDGIKVIPLSGVYIPRVNDVVIGKIVDRSSLSWDVDINSCFSAHLPAQDVFGRDFSPA
                     RDDMNRELATGDLITARIIAFDRTRDPMLTIQDRDLGKIHKGESLKISATRVPRLIGK
                     RGSMIQTIEQATQSRVLIGQNGIVVVTGRTVDGIEMAVKAIRMVEEEAHTSNLTQRIK
                     VLLNVPDTTPLPQSAPASTEASSSKTELSEPSNQVSSTTAQTGVQSNPTGIKESRKES
                     REPERHTQSSTQQIAHEPLDAPVLPSDSSSSFSADLSLPSSSSEGHSQDEKSGKADDV
                     HSAAIPTGSTEVRKTKNAVKGAQEGLQEHGKKEEGT"
     gene            1121..1864
                     /gene="rrp41"
                     /locus_tag="VE308_00160"
     CDS             1121..1864
                     /gene="rrp41"
                     /locus_tag="VE308_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HZA99413.1"
                     /translation="MSQTRSLIDKQGKRTDGRTAEQIREVKITVGVVKNADGSAFIEF
                     GKNKIVVAVYGPREVHPKHMAQPDRCVLRCRYHMSPFSTDTRKNPAPSRREVEISKVM
                     REALEPALLLEDYPRAAIDVYVEVLQADGGSRCAGITAASVALADAGINMRDLVAACA
                     AGKVDDKIVLDINDTEDKEGGADMPVAYMPHLDEVTLLQLDGILTKEQFTECFDKAVE
                     GCKMVYEVQKQALMQKYFGNETELKEEAQ"
     gene            1866..2693
                     /gene="rrp42"
                     /locus_tag="VE308_00165"
     CDS             1866..2693
                     /gene="rrp42"
                     /locus_tag="VE308_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HZA99414.1"
                     /translation="MSTSKRSTIIVEHLRRQQMWDFISKGKRLDGRRLDEMRPLEIEL
                     DIIKKANGSARVRLGDSEVVAGIKAETGEPFEGLENKGALIISAEVLPTASPYIEPGP
                     PDEETIELARVVDRGIRESEMLDLDKLVLVPGKIVYTVFVDCSVLNTDGNLLDATSYA
                     VVSALLSSNLPVFEMQDGKVVDTGKLQDPPLTTVPVSITAVRIGDTILLDPTAEEEAC
                     MNARITITTNSNSEYAAIQKGFAGGFTRDQIKKAAEVARIKGEEVRAKLQELTNKAG"
BASE COUNT          897 a          553 c          681 g          619 t
ORIGIN      
        1 tgtaaaggat gcaagaactt aaaagaaaat atgtaatacc tggcgataag atagttgacg
       61 gcaatttcag gccatcgatg aatgtgatta gggtcggaaa ttccattttc tcaacgagga
      121 ttggaattgc cgagaccgga agggacggga tcaaggtcat accattgtcc ggagtctata
      181 ttccacgggt aaacgatgtg gtaataggta aaattgtcga tcgttcttct ctttcatggg
      241 atgtagacat aaattcatgc ttctcggcac acttacctgc acaggacgtg tttggaaggg
      301 atttttctcc agcaagagat gatatgaaca gagagctggc gacgggcgac cttatcacgg
      361 ccagaataat agcgtttgat aggacaaggg atcctatgct taccattcag gacagagatc
      421 taggcaaaat acataaaggt gagtccctga agatttccgc aactagagtt ccgagactca
      481 ttggaaagcg tggctctatg attcaaacaa tagaacaggc aactcaatcg agggttctca
      541 taggtcaaaa tgggatcgtt gtggtaacag gacgaaccgt ggatggaata gaaatggccg
      601 taaaagcaat aagaatggtt gaagaagaag ctcatacttc taacctgaca cagaggatca
      661 aggtactttt gaatgtgcca gatacaacgc cactaccaca atctgcacct gcatcaactg
      721 aagcatcttc atcgaaaact gaactatcgg agccatctaa ccaggtttcc tccacaacag
      781 cacaaaccgg ggtacagtca aatccaacag gcataaagga gtcacgcaaa gaaagccgag
      841 aaccggaacg acatacacaa tcatcgacac aacaaatagc acatgaaccc ttggatgctc
      901 cggttttgcc ctctgattct tcttcctctt tctcagctga tttatcattg ccatcttcga
      961 gttcggaagg tcacagccaa gacgaaaaga gcgggaaggc agatgacgtc cactctgccg
     1021 ccatccctac aggaagtact gaggtacgaa agactaaaaa tgccgtgaaa ggagcacaag
     1081 aaggattgca agaacatggc aaaaaggagg aaggtacata atgagtcaga caagaagcct
     1141 aatagataaa caaggaaagc gaacggatgg tcgcactgca gaacagattc gtgaagtaaa
     1201 aattactgtg ggtgttgtaa aaaacgctga tgggtctgca tttatagagt ttggcaaaaa
     1261 caaaattgtc gttgctgtct atggaccaag agaggtacat ccaaaacata tggcgcaacc
     1321 agacagatgt gtccttcgat gtagatatca tatgtctcca ttttcaactg atacaagaaa
     1381 gaatcccgct ccttcccgga gagaggttga aatttccaaa gtgatgcgcg aagccttgga
     1441 gcccgccttg ttgttggaag attatccccg tgcagctatt gatgtatatg tcgaagttct
     1501 gcaagccgac ggagggtcaa gatgtgccgg tattactgct gcctctgtcg ctttagccga
     1561 cgcaggaatt aatatgcgag acctcgtagc agcatgcgcc gccggaaaag ttgacgacaa
     1621 gattgtcctt gacataaatg ataccgaaga taaagaaggt ggagcagata tgcccgttgc
     1681 ttatatgcca catcttgacg aagtgacttt acttcaactc gatggaatac tgacaaaaga
     1741 gcagttcact gaatgctttg acaaggccgt agaaggctgc aagatggtat acgaggttca
     1801 aaaacaggca ctaatgcaaa agtattttgg taatgaaaca gagctaaagg aggaagcaca
     1861 ataacatgag tacgtccaaa cgctccacta taatagtcga acacctacgc agacagcaaa
     1921 tgtgggattt tatttcaaag ggaaaaagat tggatggtag aagactcgat gaaatgagac
     1981 cgctagaaat cgaactagat atcataaaaa aggcaaatgg ttccgcaagg gtcaggctgg
     2041 gagattcgga ggtagttgct ggtataaagg cagaaacagg ggagcccttc gaaggacttg
     2101 agaataaggg agcattgatc atctcagcag aagtgctgcc tactgcttcc ccatatatcg
     2161 aaccaggacc acctgatgaa gaaacaattg aacttgcaag agtagtcgat agaggtattc
     2221 gagaatccga aatgcttgac cttgataagt tagttctggt tccagggaaa attgtataca
     2281 cagtatttgt agactgtagc gttcttaata ctgatggcaa cctgttggat gctacatcgt
     2341 atgctgtagt ctctgcattg ctgagcagca atctccctgt ttttgaaatg caagatggca
     2401 aagttgttga tacgggaaaa ttgcaagacc ctccccttac aacagtaccc gtgtccatta
     2461 cagctgttag aataggcgac acaatacttt tggatccgac cgccgaggaa gaggcttgca
     2521 tgaacgctag gattacaata accacaaact ccaacagtga gtatgcagca atccagaagg
     2581 ggtttgccgg aggattcaca agagaccaaa tcaaaaaagc agcagaggta gcaagaatta
     2641 aaggcgagga agtcagagcc aaattgcagg agttgaccaa taaagctggt tagcagaaga
     2701 agaagaagtg cggcagcatt aaagggtctg ggcgtaaggt atggagcaac
//
LOCUS       DASQTF010000007         9070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_280134,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000007 DASQTF010000000
VERSION     DASQTF010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280134"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VE308_00170"
     CDS             complement(<1..610)
                     /locus_tag="VE308_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99415.1"
                     /translation="MAKALTVAIAVILLASNFTIINAQQQGQQLTSQPGEIENRTTTA
                     TTFQSTNDSFRVQVPQGWIIQDLNNTDSALSEEATQGYEMLAQLCSEGEEQQQQGGGA
                     VLSTNASGGSTNSTSNSCQDAQGEVIHIIRYPNVETTIQEANNLTTNNNMTTNNLTTY
                     HLQKLEEVGYTNIQTVNSTETTVNLTIPQTNQTAAAMPAKLVE"
     gene            1345..1482
                     /locus_tag="VE308_00175"
     CDS             1345..1482
                     /locus_tag="VE308_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99416.1"
                     /translation="MDYNYYITYRICEYGLTTIPEEEELIWSEVFGKVGIYEEEIGFY
                     I"
     gene            complement(1528..3015)
                     /locus_tag="VE308_00180"
     CDS             complement(1528..3015)
                     /locus_tag="VE308_00180"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZA99417.1"
                     /translation="MTKLLVLKATLTYDKGTIVIRGLAHIPFAILDPRTNMLRALALH
                     YSNIIEYLKQSGIEYSDHVLSEETMMLSSSLSSSSPLSLRDYQQRALDNWHRAGLRGC
                     IVLPTGSGKTIIGIKAIEKANAASLVIVPTLDLMDQWTTTLSKYFSNVRIGNLGGGND
                     DVQSITVSTYDSAYIRAPFLGNKFSLVIFDEVHHLAAIGYRTIAEQMAAPFRLGLTAT
                     IEREDDLHKDLPRLVGEVVFQTSPDELARDKYLASYEIERRMVEMLPDELEEYRRNMS
                     IYQQCIKKLSFQRYPISLEKLIIMSGKNRAAREALLARNKAMNVALNSRAKIEELREI
                     LAENKGAKTIIFTQHNSLVHEISDKFLIPFITHKTIKEERQDVLKGFKEGRFMAIVTS
                     KVLDEGVDIPDAELGVILSGTGSAREFIQRLGRLLRPKPYNNSDSSNGITTNRYKGYK
                     KARLIEIISSETKETITSAKRKRALKIMRNNDYDHATEEMKRRSM"
     gene            complement(3340..4290)
                     /locus_tag="VE308_00185"
     CDS             complement(3340..4290)
                     /locus_tag="VE308_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99418.1"
                     /translation="MRVHLIVSFTFASNGSYPIPLANEKNANITIEHATSGIGATGII
                     GTTSHGNIQLLPDDPNGFMNISNISIELPFLIESSEPGLSFSMMSNMDTRPDIQEIKK
                     HCLECLNRLIDVVRYNTKRYWMRTLKEQDLFIYKITREKNGREEDELFFSPPSGYNYP
                     IQIIDEDSVKPSILEMLQKRTPLPLSLKLWLDGLSLYSSCRFNEAVINANISFEVFLD
                     EYLSEKYKAAGKSDEEIEQLLAKDFKDGMHKLMRRQFFASQSHEDLTKTGDPIYLKFD
                     NFRKKRTNAIHHRMRNITMPEALQVLRESRDVVIWVMDRR"
     gene            5042..5413
                     /locus_tag="VE308_00190"
     CDS             5042..5413
                     /locus_tag="VE308_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZA99419.1"
                     /translation="MTEEPEEQLRTGATCTVVDVWELLGRRWSLHILKNLKTKEVTRF
                     NELKRSLSGISSTVLSERLLELEREGLVTKKIYPEVPPRVEYSITPQARELGVIIKDL
                     ARWANKWKRPEIKPLAAQNKN"
     gene            complement(5410..6096)
                     /locus_tag="VE308_00195"
     CDS             complement(5410..6096)
                     /locus_tag="VE308_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99420.1"
                     /translation="MAETAGGAGANIAAGGRYPYYYGPEGRLVLVDSALEAVNRGSTT
                     IGIKTPAFALLASHIKPVRPLIDPAEKIFVVDGHIGATGSGYIGDILQLIDELRLEAQ
                     KHRLNFESPIDVGSLAKHLGSFLHNYTVYTVRPQAASVIIAGFDQTGVQLYQVDPSGT
                     FFRGSGFAIGKSSDAALDVVQSEYSADMSVEQAVALSNKAIERALGERPIVEIGVVSA
                     KDSRRFQKLH"
     gene            complement(6100..6681)
                     /locus_tag="VE308_00200"
     CDS             complement(6100..6681)
                     /locus_tag="VE308_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HZA99421.1"
                     /translation="MTTIVGIKTKEGVVLGSDKRASKGFFIGSKITQKIAKVDDTLAV
                     AIAGQLSDAEYLIKVAKAERQLMELRRGFPLTAKESARLIANLAYSGLKNYQPYFVEL
                     LVAGVDQDGGHVYAADMSGAITSEDFASSGSGSPIAYGVLESMYHKEITNEQAEAMAT
                     KAVAAAMERDPGSGNGIEVLTIPNLLTTVEVKK"
     gene            7040..7657
                     /locus_tag="VE308_00205"
     CDS             7040..7657
                     /locus_tag="VE308_00205"
                     /inference="COORDINATES: protein motif:HMM:NF025175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HZA99422.1"
                     /translation="MTLTTLHVAFVWMLKLRFRKLHFVALTVGAVIPDIEPLVAWVFG
                     WSVFCGWDFPCSLAPDRLVLHSIVGAITIDVVLTLIFVKIIGKLGIERLGICGFSNVK
                     TNAAFFMSAAIGSLSHVLVDWLHHPANPIFWPFFVDGSYYVDGLLLSFMAVLPASIIV
                     ATISAAITVAVIARTLDKSGYSFWLVLSNPAKALSLITESLGKAN"
     gene            complement(7650..8396)
                     /locus_tag="VE308_00210"
     CDS             complement(7650..8396)
                     /locus_tag="VE308_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZA99423.1"
                     /translation="MQVESDIPTESTTLKEKAHTEDLAAEKQVINSKQVTAAQILVED
                     AHDSQDSLSATDFHDNDNKILALLNEAGSNYSFKGLMRKLNLHQQSLSRALHRLEEME
                     LVEKSPVGYQLSKAGATAMSRTELLQQHKPKGGEYMQLLQTYIPLNVKASEVMRYLIG
                     KWFKNLRWIGMIGSDTGYMLQWTSDDGSFQINLRLIADYIVIETNASTEKEKVQAMVG
                     SYTIYEQITKILQSRLDAYVLDHRDTSVIN"
     gene            complement(8465..8752)
                     /locus_tag="VE308_00215"
     CDS             complement(8465..8752)
                     /locus_tag="VE308_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="in yeast this protein is involved in start site
                     selection during the initiation of translation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HZA99424.1"
                     /translation="MSDYDSMDELIKEIEKEEAAIVITKDIRKYRKPVTVISGLQERK
                     DLKEITKQLKTKIGTGGTFKEGQIILQGDHRETAKSLLAQMGFKEEAIEVY"
     gene            complement(8801..>9070)
                     /locus_tag="VE308_00220"
     CDS             complement(8801..>9070)
                     /locus_tag="VE308_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZA99425.1"
                     /translation="MKVTPQLARYYGLPVIDGALIARVEPYSPADDAGLRKGDIIEEI
                     DGNTIKDPSEIASYVRKKQVNDRLTVTINRYGRQFQLPVPVDERP"
BASE COUNT         2387 a         1979 c         1810 g         2894 t
ORIGIN      
        1 tttccacaag tttagctggc attgctgctg cagtttggtt tgtctgtgga attgtaaggt
       61 tgactgtcgt ctctgtactg tttacagtct ggatgttggt gtaaccaact tcctcaagct
      121 tctgtagatg gtatgtagta agattattag ttgtcatgtt attgttggta gtaagattat
      181 tagcctcttg gattgtggtc tcaacattgg gatatcgaat aatgtgtatc acttctcctt
      241 gagcatcttg acagctatta ctggtgctgt tcgtgctgcc gccactagca tttgtagaaa
      301 gtactgctcc gcctccttgt tgctgttgtt cttctccttc tgagcatagt tgagctaaca
      361 tttcatagcc ttgtgttgcc tcctctgata aggcagagtc tgtattattc aggtcctgaa
      421 ttatccagcc ctgtggcacc tgtactctga agctatcatt tgtactttga aatgttgttg
      481 ctgttgttgt tctattctca atttctccag gttgactggt caattgttgt ccttgttgct
      541 gtgcgttaat tatcgtaaaa ttagaagcta aaagaataac agcaattgct actgtcagtg
      601 ctttagccat cgctggtgaa accaataagg gtaagcgggt tctcataata accaggacaa
      661 gagacgggtt ccaaagcaag ataaggctat agaatgatta ttctgtaaaa atactcagcc
      721 ggtaacggct ttaacgactt catctttatg cctttgtatt cacatcagca aggacccaag
      781 cgctgagaga tggcatcgat aatacatctg catcgtctct gtgtctgaag gtctagcaga
      841 tagaagggcg agatcgagaa ccctccctgc agttctcaaa ctgccctttt ctgtatgttg
      901 tcagtttttc tttctttctt tccttttttg gtatgttgcg gcagttactg aagccctagt
      961 acagatacaa aatatttagt ataaatctat tactctttgt tcctctatgt gagatatcgt
     1021 agcttaagcc gaaagtctat tgaactccca gttgagctta tcaaattaga tatatatcct
     1081 ttaacaagga cattatcaag tctttccttg tggctctaaa gctcctaata atgataatca
     1141 catgatgtca aatcaaaata aaaaagggta ggaccgtaag agagagcgtg tgtatactaa
     1201 atgcatatat caattccaca tactacttct gtattcttta caagatttgg agaaactctg
     1261 atgctcactg ttttgattgg atcctttgga tgtacgtgtt gtacaatcgt gtaacctaga
     1321 gagaattagt cagcgcatat tagaatggac tacaattatt acattactta tcgcatttgt
     1381 gaatacgggc taacaacaat accggaagaa gaggaactga tatggtcaga ggtttttggt
     1441 aaggtgggta tttatgaaga agagattgga ttttacattt aggatcatcg atagagcttt
     1501 agattatagg atataagcta tatgtttcta catactcctc ctcttcattt cctctgttgc
     1561 atgatcataa tcattattac gcattatctt caaagccctc ttcctttttg cactcgtaat
     1621 tgtttctttt gtctctgaag atattatctc gatcagcctg gctttcttgt accccttata
     1681 acgattagtg gtgataccat tgctgctgtc actattatta tatggcttag gtctcagcaa
     1741 tcttccaagt ctttgtatga attctcttgc acttccagta ccgcttagta ttacacccaa
     1801 ctctgcatct ggaatatcca cgccttcgtc tagaaccttg gatgtgacta tggccatgaa
     1861 tcgaccttcc ttgaacccct tcaggacatc ctgtctttcc tccttgattg ttttgtgagt
     1921 tataaatgga attaggaatt tatcagatat ttcatggaca agactattgt gctgagtaaa
     1981 tattatcgtc ttggcacctt tgttctctgc aaggatttct cttagctctt ctattttggc
     2041 tctgctgttc agcgcaacat tcatcgcctt gtttctagct agcaaggctt cccttgctgc
     2101 cctgtttttt ccagacatta ttatcaactt ttcaagggaa atcggatatc tttgaaaact
     2161 gagtttcttt atgcattgct gatagatgct catatttctt ctgtactctt ctagctcatc
     2221 tggcagcatt tctaccattc tcctttcaat ttcgtaggac gcaaggtatt tgtctctagc
     2281 aagctcatcc ggactggttt gaaacacaac ttcgccaacc agcctcggta ggtctttatg
     2341 aagatcatct tctctttcaa tcgtagctgt aaggcctaga cgaaatggtg ctgccatttg
     2401 ctccgctatt gttctgtaac ctatagctgc cagatgatgc acctcatcaa atattacaag
     2461 cgaaaacttg ttgccaagaa aaggtgctcg tatgtaggcc gagtcatatg tcgacaccgt
     2521 aattgattgt acgtcatcat tacctccgcc gaggtttcct attctgacgt ttgaaaaata
     2581 ttttgagaga gttgtggtcc actgatccat caagtcaagt gtcggaacga taaccagcga
     2641 agcggcattt gctttctcta tggccttaat gccaataatg gttttgccag aacccgtagg
     2701 caaaacgata catcctctca aacccgcacg atgccagttg tcaagagccc tctgctgata
     2761 atccctcaat gatagcggtg atgacgatga aagagacgat gacaacatca tcgtctcttc
     2821 tgataaaaca tgatctgaat attctatacc actctgcttg aggtattcta taatattaga
     2881 ataatgtaga gcaagtgctc ttagcatgtt tgtcctcggg tctaatatgg caaatggaat
     2941 atgggcaaga ccccggatca ctatagtgcc tttatcataa gttaacgtag ctttgagcac
     3001 tagtaatttt gtcaaaaaag cattaaaagt cttgccctca atattcgatt cttgtatcga
     3061 gatgcaattt atagcctcgg gtttggtttg cccaagtcag gagggcttta ttgaatacaa
     3121 gcatattaat gcaattacgc tacgcatcca gcacaagaat aagctaatag cagcggtgac
     3181 caagaaaaca atctagacag actttcaagt tgactttgga gcaaaaacat gcgcagaaat
     3241 actctttatg aaaatgtcaa atagtagatt aaagaaatcg aagaatacga aaagattatc
     3301 atatatgcca tcctcaaagt tatttatcta taatagaact tatcttctat ccataaccca
     3361 tataacaaca tctctagact ctcttaatac ctgtaacgct tctggcatgg tgatattcct
     3421 cattctgtga tgaattgcat ttgtcctctt ctttctaaag ttatcaaatt tcaggtaaat
     3481 aggatctcca gtttttgtca aatcctcatg actttgacta gcaaaaaatt gtctcctcat
     3541 aagcttatgc ataccgtctt taaagtcctt tgctagcaat tgctcaattt cctcatcact
     3601 ttttcctgcc gccttgtatt tttcgctcag gtattcgtcc aaaaatacct caaatgagat
     3661 attagcattg atcacagctt cgttaaagcg acatgagctg tataagctta gaccgtcgag
     3721 ccataacttc aaagacaaag gtagtggtgt tcttttttgc aacatttcca agatgctggg
     3781 tttcacacta tcttcatcaa tgatttgaat tgggtaatta tagcctgaag gtggcgaaaa
     3841 gaaaagttca tcttcctctc ttccattttt ctctctagtt attttatata tgaataaatc
     3901 ttgctctttc agggtcctca tccaatatct cttcgtattg tatcttacga cgtctatcag
     3961 acgattaagg cattccaaac agtgcttttt gatctcctgt atatctggtc gggtatccat
     4021 attcgacatc atactaaagg ataaacccgg ttcagatgat tctatcagaa atggtagttc
     4081 tatactaata tttgaaatat tcatgaagcc attaggatca tctggcagaa gttgaatatt
     4141 tccatgactt gtagttccta taataccggt tgcgcctatc ccacttgtgg catgctcgat
     4201 ggttatgtta gcattttttt cattagctag tgggataggg taagaaccat ttgatgcaaa
     4261 ggtgaatgat acaattagat gtactctcat ttggtccatt attttgaaac gaattgattt
     4321 aagttaataa acatagttgt ctaaggtctt gatcatgatt tgtaggagcc atcaaatcgg
     4381 atggtgtaga attgttattt acaagtttgc gatactgttt gaagcatggt ctatgaaaaa
     4441 ataaggcatc tataaacagg aggtcaaata atactttgag atttcgagtt aattcacggg
     4501 cttctagagc tgtttgaagg aaaggtttca aaaggaaaca gtctataagt tcaagaatgg
     4561 tatcacaaag aaggctagtc gttgtcaata aataactatt gtcaagatcc atattgaaac
     4621 agttcaggtt gtcatatgga aagtccattc aactattttc aattcttcaa ttcctctccg
     4681 cagtctatgc acgatagttg ggagaaatga aaagccatct tcaatccgat atggttagta
     4741 gatgaataga tattcatttt cgaatacttc attgaggaca ttaatttttt gagttctata
     4801 tactgcttta ggcctccttc atttttcttt ctagatgctg gcccattcta actatggagt
     4861 agtttcaggg tcaagacttg tcagcacaag ctatcagatg ctttaatgca ggcttgctta
     4921 gaggaattag ccaagataaa cacttttcaa tttcaatttt acaacattaa ctactagcca
     4981 ttgtgattgc agccagtgtc tccacataat tttaatagca agtaagtaat cactactagg
     5041 tgtgacagaa gaaccagaag aacagctcag gacaggtgct acatgtacag tggtggatgt
     5101 atgggagctt ctaggtagaa ggtggtcact ccatattttg aaaaatctaa aaacaaaaga
     5161 agtcactagg tttaacgaac tgaaaagatc gctgtccggc atcagcagca cggtactatc
     5221 ggagcggctg ctggaactag agcgtgaagg gcttgtgaca aaaaagatct accctgaagt
     5281 gccaccaagg gtagaatata gtatcacgcc tcaggcaaga gagctaggag tgataataaa
     5341 agatctggcg cgttgggcaa acaagtggaa gcgcccagaa ataaagccac ttgctgccca
     5401 aaataaaaat tagtgcagct tttggaacct gcggctgtcc tttgcgctga ccacccctat
     5461 ttccacaata ggtcgctcgc ccagtgccct ttctattgct ttattactca atgcgacagc
     5521 ttgctcaacg ctcatgtcgg cgctatactc gctctgtacc acatctagtg ctgcatcaga
     5581 cgacttacct attgcaaagc cagagcctct gaagaatgtc ccgcttgggt caacctgata
     5641 gagttgcacg ccagtctggt caaagccagc tatgatcaca gatgcagcct gtggcctaac
     5701 tgtgtatacc gtgtaattgt gcaggaacga ccccagatgc tttgcaagcg agcccacatc
     5761 tatcggactt tcaaagttga gccggtgctt ctgtgcttca agtctcagct cgtctatcag
     5821 ctgcaatatg tcgccaatat atcctgagcc ggttgcacct atatggccgt cgactacaaa
     5881 tatcttttcg gccgggtcta tcagcggcct gacaggcttg atgtggcttg ctaggagagc
     5941 aaacgctgga gtcttgatcc ctattgtggt tgatccccta tttactgctt caagggcgct
     6001 atcgactagc accaaccggc cttccggccc ataatagtac ggataccttc ctccagcagc
     6061 aatgtttgct cctgctcctc ctgctgtttc agccatcccc tactttttca cctccactgt
     6121 tgtcaacagg tttgggatgg taagcacctc tatgccgttg ccagagccag gatccctctc
     6181 catcgctgca gccaccgctt tagtagccat agcctcagcc tgttcatttg tgatttcttt
     6241 gtggtacatg ctctcaagca ctccatatgc aataggcgaa ccggagcctg acgaggcaaa
     6301 atcttcgctt gttattgctc cactcatatc agctgcatag acgtgcccgc catcttggtc
     6361 aacgccggcg actaacagtt cgacaaaata aggctgatag ttctttaggc cggagtaagc
     6421 aaggtttgcg atcaatcttg ctgattcttt ggctgtgagc ggaaagcctc ttcgcaactc
     6481 catcagctgg cgctctgcct tggcaacttt aatcaaatac tctgcatcag ataactgtcc
     6541 tgctattgcc acagcaagtg tgtcgtctac cttagctatc ttttgcgtta tttttgagcc
     6601 aatgaaaaag cccttgcttg cccgcttgtc agagcctagc acgactcctt ccttggtctt
     6661 gataccaacg attgttgtca tttctgatcg tagtataaca agataggaat taattgacct
     6721 gtattgatgg aaataagtca cagctatttt tgtctagtta aactctttgt aaataatgca
     6781 tctattacca agcgcttagc ggatcaatcc gagcgtttat cgctaggtca gatatagttt
     6841 gtttgaattg tgcaggcaat aaagtctacc gcaaatagac taaatgacag tttattgttt
     6901 tcaagaggag tcgtactcac ctctatacat actaaatcct acagaggatc aataaaagaa
     6961 taatctaaaa gaccatggaa gaccctgttt gacaaaaaat tcttatggat aaaaataata
     7021 caattgacaa aataaattca tgacgcttac cactcttcat gtcgcctttg tctggatgct
     7081 taagctacga ttcagaaaac tgcatttcgt ggctctgaca gtcggtgcag tcatccctga
     7141 tatcgagcct ctggtagcct gggtgtttgg atggtcggtg ttttgcggct gggactttcc
     7201 atgctcgctt gctcccgaca gactggtttt gcactcaatt gtaggagcca taaccataga
     7261 tgttgtcctt acgctaattt tcgtcaaaat tataggcaag cttggtattg aaagactggg
     7321 gatatgtgga tttagcaatg tcaagacaaa tgctgctttt tttatgtctg ctgcgattgg
     7381 cagcctgtcg catgttttag tcgactggct ccatcaccct gcaaatccga ttttctggcc
     7441 cttttttgta gacggctcgt attacgtaga tggactgctg ctttcgttta tggccgtttt
     7501 acctgcctcc atcatcgtgg caactatatc tgctgcgatc acggtagcag tcatagcaag
     7561 aactcttgac aagagcggct atagcttctg gctggtactt tcaaatcctg caaaggcact
     7621 atctttgata acagaatcac ttgggaaagc taattgatta cactagtgtc tcggtgatcc
     7681 agtacgtacg catcaagtct gctctgcaat atctttgtta tttgctcata gatggtatac
     7741 gaccccacca tcgcctgcac tttttctttt tcagtggatg cattggtctc tatgacgata
     7801 taatctgcaa tcaatctaag gttaatttgg aacgagccat cgtcgcttgt ccactgcagc
     7861 atataacctg tatcgctccc tatcatgcct atccatctaa ggttcttaaa ccacttgccg
     7921 atcaagtagc gcataacttc tgaggcctta acatttagcg ggatatacgt ctgcaataac
     7981 tgcatatact caccgccctt tggtttgtgt tgttgcaata attcggtcct tgacattgca
     8041 gttgcaccag ccttgctaag ctgataccct actggagact tttccacaag ctccatttct
     8101 tctagcctat gaagcgccct tgacaagctt tgctggtgaa gattaagctt acgcatcaag
     8161 cctttaaatg agtagtttga tccagcttca ttcagtaatg ccaagatctt gttatcgttg
     8221 tcatgaaaat cggttgcact aagcgagtcc tgtgagtcat gagcatcttc taccaggatc
     8281 tgagcagccg tcacctgctt gctgtttatc acctgctttt ccgctgcaag gtcctctgta
     8341 tgggccttct ccttgagggt cgttgattca gtcggaatgt cgctttccac ttgcatatta
     8401 caagcgcccc ttcatgatat aaatcataaa gtaaccatcc ggagagcagg taagtaaaat
     8461 gctactaata cacctcaata gcctcttcct tgaaacccat ctgagccagt agactctttg
     8521 ccgtctctcg atggtcccct tggagaatta tctggccttc cttgaacgtg cctcctgtgc
     8581 caatcttggt ctttagctgt tttgtaattt ccttcaaatc ttttctctcc tgaaggcctg
     8641 atatcacagt gacaggtttc ctatactttc tgatgtcttt ggtaatcact atggctgcct
     8701 cttccttttc aatctctttg atcagctcat ccattgaatc atagtcagac actaggatga
     8761 ttattgccgc tcacgttcaa aagtgttttg ataaaatatt ttatgggcgt tcgtcgactg
     8821 gaactggaag ttgaaactgg cgcccatagc gattgatagt aactgttagt cggtcattta
     8881 cctgcttttt gcgcacgtat gaagcaattt ctgaagggtc ttttatcgtg ttgccatcta
     8941 tttcttcgat gatatctcct tttcttagcc ctgcatcgtc agccggacta taaggctcaa
     9001 ctcttgcaat gagcgctcca tcaatcacag ggagcccata gtaccgtgca agctgaggtg
     9061 taaccttcat
//
LOCUS       DASQTF010000008         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_280383,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000008 DASQTF010000000
VERSION     DASQTF010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280383"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..1180
                     /locus_tag="VE308_00225"
     CDS             398..1180
                     /locus_tag="VE308_00225"
                     /inference="COORDINATES: protein motif:HMM:NF022932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZA99426.1"
                     /translation="MSISDRSRKAMENLGLTGYEIRVYLSLLDTGSMTAADISKKSGV
                     PYSKIYEVLNSLEEKGWLESGSSRPQKFFPKSPSTALGIMRTRHENNFRESQSTIVNE
                     LMPMYTKSGIKERPEIWVARGVYNIIAKVNEIIQNVQQELLVALPFVAVDIAKPLQPE
                     LRTLHDRGAVKINILASAKVSPDTVKTLSRVAEVRLKDGLFGGGIIGDAKHVVILLGE
                     GTGENGSFEPIAIWADHAGLAGFAKGYFQYLWEDAGEKKQSR"
     gene            1241..1711
                     /locus_tag="VE308_00230"
     CDS             1241..1711
                     /locus_tag="VE308_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZA99427.1"
                     /translation="MESVVAEKKQQMTEEEELYVGTAESEHIEMYLKAIWCIRERGEE
                     VKVSSIAKLLNVTQPSVVQMLRKLNRSQFVRYSKGSVEMTTEGGHIGKKMMRNTRLLE
                     VLMKDALKIEVDEEMICGIEHHMKDMFTDALCTLLKHPRKCPHGHEIPKGKCCS"
     gene            1866..>2250
                     /locus_tag="VE308_00235"
     CDS             1866..>2250
                     /locus_tag="VE308_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99428.1"
                     /translation="MVKIETTASLESFRKFIIISTCRSFIPESYLRDYEVFPEREEGP
                     GAIYIEAADKVTLKKIREMTFVNAKEILGIIYSSKSGNTQLKWRQTRRRIGKVQGFAS
                     PNALVNLVESGVLTQEWVDNYLKLIQ"
BASE COUNT          696 a          457 c          540 g          557 t
ORIGIN      
        1 accttaccct ctctttcaaa gacctcagca tccagaatca tattgcagtc tggacagatg
       61 ctttgtgtga accggattgt gcgtctagcg ctcacacttt tgctagacat gttcgaaatt
      121 tggattaagt ccatactact gttacctttt ggacacaatc ttcgctttga tataaagcta
      181 acttactaat tcctacggtg atgaataggt ttataatcac gcatgatcat aagaaacatt
      241 ctcagttcta catcgaagtt tgtgtgaatc tcgttctctt tttgagagaa tgaactacct
      301 tttaacctgc cagccatgga tgttgtcact ttttactgaa aagcgcatat acatttaaaa
      361 agcaaaaaat atactacact gcccggtttg agaagaagtg agcattagcg ataggtcaag
      421 gaaggcgatg gaaaaccttg gcttgacagg ttatgagata cgcgtttacc tttcactgct
      481 tgacacaggc tccatgactg ccgcagatat ttcaaaaaaa tcaggggttc catactcgaa
      541 gatctatgaa gtcctcaata gcctagaaga gaagggatgg cttgagtccg gcagctcgag
      601 gcctcaaaag tttttcccca agtctccctc tacagctctt ggaataatga ggacgcgtca
      661 tgagaacaac ttccgcgaga gccagagcac tatagtaaac gagttaatgc caatgtatac
      721 aaaaagcggc ataaaggaga ggcctgagat atgggtcgca agaggagtct ataacataat
      781 cgccaaggtc aatgagataa tccagaacgt gcagcaggaa ctgctggtcg cgctaccctt
      841 tgttgcagta gacatagcca aaccactaca gccagagctc aggaccttgc atgacagggg
      901 agctgtcaag ataaacatcc ttgcatctgc caaggtaagc cctgatacag ttaagacatt
      961 atcaagagta gcagaggtga gactcaagga tggtcttttt ggaggcggca taataggcga
     1021 cgcaaaacat gtcgtgatac tccttggaga aggcacaggc gagaacggca gctttgaacc
     1081 gatagctatc tgggctgacc atgcaggcct tgcaggcttt gcaaaaggtt acttccagta
     1141 cctatgggaa gacgctggcg agaagaaaca atctaggtaa aaacggcgtt atacacattc
     1201 agataaggtt aatacagttt ggcgagaaag aataaccact gtggagtcag tcgttgccga
     1261 gaaaaagcag caaatgaccg aagaggaaga gttgtatgtt ggtacagcag agtctgagca
     1321 tattgagatg tacctaaaag caatatggtg tatccgcgaa agaggcgagg aagttaaggt
     1381 gagctcaata gccaagcttc taaatgtcac ccagccatct gtcgtccaaa tgctccgcaa
     1441 gttgaataga tcacaatttg tgcgctacag caaaggtagc gtggaaatga ctacagaagg
     1501 aggacatatt ggcaagaaga tgatgcggaa caccagactg cttgaagtcc taatgaagga
     1561 tgctctcaaa atagaggtag acgaggaaat gatctgtggg atagaacatc atatgaagga
     1621 tatgttcaca gatgcattgt gcaccctttt gaagcaccca cgtaagtgcc ctcacggcca
     1681 cgaaattcca aagggcaagt gctgttcgta gatttttatt cttgcagtcc ctcattttag
     1741 tgtggacaag cgatgactag atagccctac tgacttgatg aaggagatct tgagtgagga
     1801 gtttgtccac tctcttacat ctcacaaaac ttgataaatg gctaggtggc ttttatatat
     1861 ctacattggt taagatagaa actacggctt cattggagag ctttagaaag tttatcatca
     1921 taagcacctg cagatccttc atacctgaaa gttatctgcg cgattatgag gtatttccag
     1981 agcgcgaaga gggtcccgga gcaatctata ttgaagctgc ggataaggtt acactgaaga
     2041 aaataaggga gatgacgttt gtaaacgcaa aggagatcct tggcattatt tattcatcaa
     2101 agagcggcaa cactcagcta aagtggaggc agactcgcag gcgaataggt aaggtacaag
     2161 gctttgcatc gccaaatgcc ctagtcaacc tagttgagtc aggtgtcctg acacaggaat
     2221 gggttgacaa ctatttgaaa ttgatccaaa
//
LOCUS       DASQTF010000009         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1020345,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000009 DASQTF010000000
VERSION     DASQTF010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020345"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VE308_00240"
     CDS             complement(<1..993)
                     /locus_tag="VE308_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HZA99429.1"
                     /translation="MKILVVSGEYPPMKGGVGRYTKNLVQAIANKSDVEVSVAIGADA
                     AVTSDPSQDSSNIVTIYRGVITKGDKRNSDRILQIVSQIKPDVVNIQYERGLYEVDTT
                     IRHTFWRLAHGSTLDRFYKMCPVPTVSTLHTVFPQDEYRAYIKERALRKEGRFGFLPQ
                     PLRASIRRWVMKQRYNLLLQVVSQSDGVISPARTLQEVVRRGTVIYHGAEPAIQLSST
                     SSPMNDKKEFRKDFGLPVDKMLLLAFGYAGSYKGFDVLDSLDLPNGWSLVVKQTKHER
                     GFERPIEIGTNNTENVISLNLGYLDDTTMSKLFFACDAIIFPYKIVSISGVMFDA"
     gene            complement(1000..2175)
                     /locus_tag="VE308_00245"
     CDS             complement(1000..2175)
                     /locus_tag="VE308_00245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA99430.1"
                     /translation="MRKAVVIHHTLNSLGGETTVAIETIESLYELGYEIELVTVQPPD
                     LDRITKSYGKKIHIARIRSLLPFKLNYFGVYQRLLTMLSSIDFKDSHVVINTHGDALP
                     YRISGNVPYLLYLHFPTFLMNSTGNYASNKYRKSLFWRAYFKPYSIITRSLAMRATKR
                     SSLILTNSRFSREAIREAFPDVHPYVLHPPVDTERFSRAYSEPINAREVKVLVVSRFS
                     PEKRIENAIKIAHLLGGKIKFQIVGSLAPANRPYFKRLQQMIEMYGLSQTVSLTPNAS
                     NEELIDSMSKSIVYLHTMIGEHFGVSIVEAMAAGLLPIVPAYGGCSEIVPTDHQYHTL
                     EEAADLIAENAKYANDEKRLKMHEISMHFSPKNFRKAMRIYIEQARSQISVYRSSST"
BASE COUNT          795 a          595 c          569 g          894 t
ORIGIN      
        1 cgcatcgaac attacgccag aaatcgacac gatcttatat ggaaatatta tggcatcgca
       61 tgcaaagaag agctttgaca ttgtagtatc atctaggtat cctaggttta gactgatgac
      121 attctctgta ttatttgtcc caatctcgat gggtctctca aaccctcttt cgtgcttagt
      181 ctgcttcacc actaaagacc aaccattagg aaggtcaagg ctatctagca catcaaatcc
      241 tttgtatgag cccgcgtatc caaacgcaag taaaagcatt ttatctacag gtaatccgaa
      301 gtcctttcta aactcctttt tatcattcat tggcgaagat gtagacgaga gctggatggc
      361 cggctctgca ccatgataaa ttactgttcc gcgcctgact acttcttgaa gtgtccttgc
      421 agggcttatt accccgtctg attggcttac tacctgaagc agtagattat atcgctgctt
      481 cattacccat cttcttatag aagcacgcaa gggctgaggc aggaaaccaa atctaccttc
      541 cttcctcaat gccctttctt ttatataagc acgatattcg tcctgaggaa aaacagtatg
      601 caacgtagat actgtgggta ctgggcacat cttgtaaaat cgatccaacg tagacccatg
      661 agcaagtctc caaaatgtat gtctgatggt tgtatcgacc tcgtatagcc ctctttcata
      721 ttgtatattc acaacatcag gttttatttg agaaacaatc tgcaagatac ggtcagagtt
      781 cctcttatcg ccttttgtta tgacaccacg atagatagta acaatattgc tgctgtcctg
      841 actaggatcg ctagtaacag cggcgtcggc acctattgcg acggacactt caacatcaga
      901 tttattggca atcgcttgca ccaaattctt ggtataccta ccgacacctc ctttcattgg
      961 aggatactcg ccagagacta ctaggatctt catccccttt taagttgagg aagatctgta
     1021 tacacttatt tggcttcttg cctgctcgat gtatatcctc attgcctttc ggaagttctt
     1081 tggcgaaaaa tgcatagaga tttcatgcat tttcagcctt ttttcatcat tagcgtattt
     1141 ggcgttttct gcaataagat cagcagcttc ttctagcgta tggtattggt gatcagttgg
     1201 aacaatttca ctgcaaccgc catatgctgg cacaattgga agaagccctg ctgccattgc
     1261 ttctacaatt gatactccaa aatgctcgcc aatcattgta tgaagataaa caatgctttt
     1321 agacattgaa tcaataagtt cttcattact ggcgttaggt gtcaaactca ctgtctggct
     1381 caagccatac atttctatca tttgctgtag ccttttaaaa taaggccgat ttgcaggcgc
     1441 aagtgaacct acaatctgaa atttgatttt cccgccaaga agatgagcga ttttgatagc
     1501 attttcgata cgtttttctg gcgagaagcg agacactact aatactttta cttccctagc
     1561 attgattggc tcgctgtagg cacgtgaaaa acgttcagtg tctacaggtg gatgtagtac
     1621 gtagggatgt acatcaggaa acgcttccct tattgcttct ctgctaaatc tagaatttgt
     1681 aagtatcaag cttgaccttt ttgtggcgcg catagcaaga gagcgtgtaa taatactata
     1741 tggcttaaag tatgctctcc agaataaaga ttttctgtac ttattgctgg catagttgcc
     1801 agtcgaattc atcaaaaatg tcggaaagtg aagatacaat agatatggca cattccccga
     1861 aattctataa ggcagagcat ctccatgggt attgataaca acatgagaat ccttgaaatc
     1921 tattgatgaa agcatagtca acagcctttg atagacgcca aaatagttca gtttgaaagg
     1981 tagcagggat cttattcgtg caatatgtat ctttttacca taagacttgg tgattctatc
     2041 aaggtcaggt ggctgaacag tgacaagttc tatctcataa ccaagctcgt ataaagattc
     2101 tatagtttcg atggcaactg ttgtctcgcc tccaagagag ttcagtgtat gatgaattac
     2161 gacagctttt cgcatcaagc gagtattgaa agatatcaat atcgcctatt tttaaagctt
     2221 ccatgtctac tgatctctct tgcgactttt gaatctgtat tgcattttct caaaattgat
     2281 gtatcttttc cgattacatt gtacaatgga tatccagtta attatgggtc tcctaatgcc
     2341 agaagccttt catagcagcc taggtcaaaa agtacctttc ttcagactct ttttttgaga
     2401 ggagctattt ttcttgagaa ataagaaaaa tggactaact tggatgtatc acattgatga
     2461 tatttggcag agtattggtt atctgtgtag tgcaataaga ctgcgccaaa atttgggctt
     2521 agaaatccga ttctttaaag ttacctggta agtctgcatt cctttttcta gaatagttat
     2581 gctcagctgc tacaaaaaca acaagaagag cggagcctgc agtcattaat agttagcagc
     2641 agattatatc agaggttttg cttcatctgc gatatgatga agcttggaag gacgtaacga
     2701 gatagaggta ttggatcagc catgtgttgc ccctctatcg tctattacaa tctctacatc
     2761 accttggtac ttgggcttga ctgcaagctg ggctggtgtg taaatgagat gctcctttgt
     2821 aaacgctgac agctcgtagt catttgtcat aaa
//
LOCUS       DASQTF010000010         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_2396,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000010 DASQTF010000000
VERSION     DASQTF010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2396"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /gene="larB"
                     /locus_tag="VE308_00250"
     CDS             complement(<1..693)
                     /gene="larB"
                     /locus_tag="VE308_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZA99431.1"
                     /translation="MDLHTILDEFANGSLSLSEVKKEISIHAIEKIGDIAKLDVGREI
                     RRGGMPEIIFSESKEYKDIIRIATAIVRRNGQVVVSRIKKNELSKVAGALRRKGLTIE
                     IGRNSTTLLASEKLFACKMTDAKIGIMSAGTSDVGVAEEARIVSKAMGCGAITSYDVG
                     IAGMHRLFPALKEMFKQEIGAIVVVAGMEGALASVVASMVDIPVVGVPTSIGYGFGAN
                     GIGALASMLQSCT"
     gene            complement(732..1679)
                     /locus_tag="VE308_00255"
     CDS             complement(732..1679)
                     /locus_tag="VE308_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZA99432.1"
                     /translation="MSNPSVASSAMTITIIGSGKVGASAALNCGLRELDRDILLLDIV
                     QGLPQGEAMDINHQLSERGSDTIVRGSNNYEDVRDSDYVVLVAGVGRKPGMTRMDLLK
                     INAGIVKDVVSKIALYAKNASVIVVTNPLDPMTYFALKTLGAEKNKVMGMGGMLDLSR
                     FKSFIQDATNISRSSIQAMVISEHGENMLPLTRFSSIGGIPLHEFISKDKAMEIFEKT
                     RKVAAEVIALKGATVYAPGNAVATMLESAIRDKKLVIPVSTLLDGEYGFYDVCIGVPA
                     IIGASGVERIIELKLDSFEQDIFSKGVASVKEAIKALPL"
     gene            1741..>2040
                     /locus_tag="VE308_00260"
     CDS             1741..>2040
                     /locus_tag="VE308_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99433.1"
                     /translation="MSKKRRDDSYFAVDRRRKRKYMKIIIPVIVAVVAAGIAGAILYQ
                     SPTAMAISGIECDRTEQLNYHIHSGLDVFVNGVQQQVPSNIGILSSPSCLYWVHTH"
BASE COUNT          528 a          514 c          406 g          592 t
ORIGIN      
        1 ggtgcagctc tggagcatag atgctaacgc tcctatgccg tttgcaccaa agccatagcc
       61 tattgaagtt ggcaccccca caaccggtat atcgaccatc gatgctacaa ctgaggcaag
      121 tgcaccttcc attcctgcca cgactactat ggcgcctatc tcttgcttga acatctcttt
      181 aagagctgga aagagacgat gcattccagc aatgccgaca tcgtaacttg ttatcgcccc
      241 acagcccatc gccttggaaa caatccttgc ctcttctgcg acgccaacgt ctgaagtacc
      301 ggccgacatt atgcctattt ttgcatcggt cattttgcag gcgaacaact tttcagaggc
      361 aagtagtgtt gtgctgttcc tcccaatttc gatagtaaga ccctttctac ggagcgcacc
      421 tgctactttt gatagctcgt tcttctttat ccggctgact acaacttgac cgttacgcct
      481 aacaatcgcg gtggctattc tgatgatgtc tttgtactct ttgctttcag aaaaaatgat
      541 ctctggcatt ccgcctcttc ttatttcccg gccgacatcg agcttggcaa tatcgcctat
      601 cttttcaatt gcgtggatgg agatctcctt cttcacctcc gataggctaa ggctgccatt
      661 tgcaaattca tcgagaattg tatgcagatc catccgtcag ccagctatca acaacaagaa
      721 aataaaaggg tttaaagtgg aagtgctttg atcgcttcct tcacgcttgc gacgccctta
      781 ctgaaaatgt cctgctcaaa gctatccagc ttgagctcaa ttatgcgctc gactccactt
      841 gcgccaatga tcgctgggac acctatacac acatcataaa atccatattc gccatcaagc
      901 aatgttgaca ctggaattac caactttttg tctcggatgg cagactctag catagtcgca
      961 actgcgttgc cgggtgcata tactgtagct ccctttagtg caatgacctc tgctgcgacc
     1021 tttcttgtct tttcaaagat ctccattgcc ttatcctttg aaataaactc atgaagcggt
     1081 atccctccta tagaagagaa ccttgtcagg ggtagcatgt tttcgccgtg ctcgcttatc
     1141 accatagctt gaatcgaact gcgtgaaatg tttgtcgcat cttgaataaa gctcttgaac
     1201 cttgataagt ctagcatgcc acccattccc ataaccttgt tcttttctgc tccaagagtc
     1261 tttagcgcaa aataagtcat tggatcaagt gggtttgtaa ctactattac actcgcattc
     1321 tttgcataaa gcgcaatttt ggacacgaca tctttgacaa tgcctgcatt tattttgagg
     1381 agatccattc tcgtcatgcc tggctttctt ccgacgccag caacaagcac tacatagtca
     1441 gaatcgcgca catcttcgta attgtttgaa ccacgcacta tagtatccga gcccctttct
     1501 gacagctggt gattgatgtc cattgcttct ccctgaggaa gcccttgaac tatatcaagc
     1561 agcagtatgt ctctatcaag ctccctcagt ccacaattaa gggcagctga ggcaccaacc
     1621 tttccagatc cgattatggt aattgtcata gctgaacttg caactgaagg attgctcata
     1681 taaattgtaa ttggagccgg atcagcttta aattaatcag catgtcaagt caataacgaa
     1741 ttgtcaaaga aaaggaggga cgacagctac tttgctgtag atcgacgtag aaaaagaaaa
     1801 tacatgaaga ttataatacc agtcatcgtt gcagtggtgg cagctggtat tgcaggggct
     1861 attttatacc agtctcctac ggcaatggca ataagtggaa tagaatgtga taggactgag
     1921 cagcttaatt accatattca ttcaggttta gatgtatttg ttaatggcgt acaacagcaa
     1981 gtcccttcaa atattgggat actctcttca ccctcatgct tatactgggt tcatacccac
//
LOCUS       DASQTF010000011         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1113081,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000011 DASQTF010000000
VERSION     DASQTF010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113081"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VE308_00265"
     CDS             complement(<1..629)
                     /locus_tag="VE308_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZA99434.1"
                     /translation="MSKAGIEVIVQPSFWLGSPRTSVGTFEDYWEHMITFETKRASDF
                     GIEHFVCISVNPKEAMERPLALDAMEAMKKYLDRERVVAVGELGYNLINDLEEDVFVK
                     QMDIAAEKDMLMTVHLPHINKPEGMRRIDLILNSERGRRYNRHKILIDHNVEETIEKT
                     LESGMWAGLSVYPVSKLSPERAMNIIKKYGTERIMIHSAADWGPSDPLSV"
     gene            763..1803
                     /locus_tag="VE308_00270"
     CDS             763..1803
                     /locus_tag="VE308_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZA99435.1"
                     /translation="MLAAVFHAPNNIIVKQVPITRTQVLLKVSACAVCGYDARVFRNG
                     HRKVRPPVILGHELCGEIQNDITVSDDMTINAGSRVAVCPLIPCLHCPYCQIGDYNLC
                     INLREIGSTSDGGFAQYVTIPEQLIRIGGLVPVPDNLTDEEAALLEPLACCLNSFSRM
                     LPVSKESSVVIIGDGPMGLIHLQLFKKLSSSRVAIVGKVPLRMEKARAMGADAVFAYS
                     RETIPDVLDFARASSGGADIVVIATSNPIAFELATKVSARNSKINVFAGMPAGQTLLL
                     DANWLHYNQISITGSFSSTPKMLGEATRIAAEKTVDLSTIVTHKFSLPEIEKAIDVTE
                     KYHGLRAVINKF"
     gene            complement(2601..2674)
                     /locus_tag="VE308_00275"
     tRNA            complement(2601..2674)
                     /locus_tag="VE308_00275"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2638..2640),aa:Ile,seq:gat)
BASE COUNT          728 a          625 c          569 g          789 t
ORIGIN      
        1 acgctgagcg ggtctgatgg gccccagtca gctgcactat ggatcattat tctttctgtg
       61 ccatattttt tgatgatgtt cattgcacgc tctggcgaca gcttagacac aggatacaca
      121 gaaagtccag cccacatgcc agactcaagt gtcttttcaa ttgtttcttc gacgttatga
      181 tctatcaaaa tcttgtgcct attgtagcgc cggccccgct cactatttaa tataaggtca
      241 atacgtcgca tcccttcggg cttgttaatg tgcggtaggt gaactgtcat gagcatgtcc
      301 ttttctgctg caatatccat ctgctttaca aacacatcct cttcgagatc atttatgagg
      361 ttgtagccaa gctctccgac agctactacc cgttccctgt ccaagtattt cttcatggct
      421 tccatagcat ctaaggcaag tggccgttcc atggcctcct ttgggtttac tgaaatgcat
      481 acaaagtgct ctattccaaa gtcacttgcc cgctttgttt caaaagttat catatgttcc
      541 caatagtcct caaatgttcc aaccgatgtc cgtgggcttc caagccagaa tgatggctgg
      601 actattacct ctattcccgc tttggacatt gcttcatagt catcggtggt ccgagagtac
      661 atatggatgt ggggttcaaa tagccttgtc atatctctac tataacaagc tgcgttgtaa
      721 ctctttttag gttgtagaac aaatttacag tatcaaaaga atatgctggc tgcggttttc
      781 catgcaccaa acaacatcat tgtgaagcaa gtacctatca ctcgcacgca ggtcctgctc
      841 aaggtgagcg cctgtgcagt atgtgggtat gacgcccgcg tctttagaaa cggtcatcga
      901 aaggtgcgtc ccccagtaat tttaggacac gagctttgcg gagaaataca aaacgacatt
      961 acagtaagcg acgacatgac tataaatgca ggttcgaggg ttgccgtttg tcccctgatc
     1021 ccttgtcttc actgcccata ttgccagatc ggggattaca atctttgcat caacttgagg
     1081 gaaataggct ctacctcaga tggcggcttt gcacaatatg tcacaatacc ggagcagctg
     1141 attagaatag gcggcctggt tccagtgcca gacaacctaa ctgacgaaga agctgccctt
     1201 ttagagcctc ttgcgtgttg cttgaacagc ttttctcgca tgcttcctgt ttcgaaggaa
     1261 agctctgttg tgataatagg ggatggaccc atggggctca ttcaccttca gcttttcaag
     1321 aaattatcga gctcgagagt agcaattgtc ggcaaggttc cattaaggat ggaaaaggca
     1381 agagctatgg gggcagacgc agtctttgct tattctcgtg aaacaattcc tgacgttctt
     1441 gacttcgcca gagcttcttc tggtggtgca gacatagtag tgattgcgac gagcaaccct
     1501 attgcctttg aactcgcaac aaaggtctct gccagaaact ccaagataaa tgtctttgcc
     1561 ggcatgccag ctggacagac gcttttgttg gacgccaact ggctgcacta taaccagatc
     1621 tctatcactg gctcctttag ctctacgcct aaaatgctag gcgaggcaac aagaattgca
     1681 gcagaaaaaa cggttgacct atccacaatt gtcacacaca agttttctct tccagaaata
     1741 gaaaaagcaa tagacgtcac tgaaaagtat catggattga gagctgtgat aaacaaattc
     1801 taatgtgatg gaaggctggt gtattgtctc cactgaactt gttatatgtg atcatcgagg
     1861 atcaaacggt gaaagctatg atttcacact tgaagtgcaa atcaaaagcg acttaaaaga
     1921 tctctacggt tttgcccttg tcacatgccc ctcattggct catgctgtag gatctcttcc
     1981 cgatcttttc tctttttggc acctctcgat ttgtatctaa gcttacttcc gcagcaaaaa
     2041 caatgagtcg cgccttcatt atgaaaataa acagcacacc tgctgcaata gttcattccc
     2101 ttattataga ggctgccatt atagcctcgc actccgactc gttgacacaa gtttctacaa
     2161 accaaagccc aagcgcctag ctgaaaccaa attgatataa aagcactatt atctaagcaa
     2221 gagaggaaaa cgtgttcttt ttgttatttt gcaggaagcc agattataaa gagtgcgcga
     2281 ttcattatat taatttctag ggcactttaa ttcttagagt aacctatctt gctcatatac
     2341 atgctcatat acacctaatc atgctattgc tttatagttc gtgcctaacc gtcagaatca
     2401 tgacaaccga ttgttgacta agcgacggat tgttagttta tgatggattc atgacactaa
     2461 tactttccaa gccaacagca tacatggcta gcatagaggc ctgtttgtat gtttcatgct
     2521 gaggctcttt gaaagatttg aaataccaat tttgctggtt acgataagag aataataata
     2581 ataataataa tatattgcag tgggccgtaa gggatttgaa ccctcgacct ttcgattatc
     2641 agtcgaacgc tcctccaagc tgagctaacg gcccggtcat ttcaaaattg ctagcgttat
     2701 ttaattgtgt a
//
LOCUS       DASQTF010000012         4930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_928130,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000012 DASQTF010000000
VERSION     DASQTF010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928130"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VE308_00280"
     CDS             complement(<1..282)
                     /locus_tag="VE308_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZA99436.1"
                     /translation="MQPNTIFEKIWDSHIVYEKEAEEGGQSLLYVDRHLIHEVTSPQA
                     FDGLRMSKRRVRRPDLTFATMDHNVPTSDRSLPIVDQISSIQIQTLAQNC"
     gene            379..1038
                     /gene="psmB"
                     /locus_tag="VE308_00285"
     CDS             379..1038
                     /gene="psmB"
                     /locus_tag="VE308_00285"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HZA99437.1"
                     /translation="MYPHDKRYPDYIADQIKKGTTTCGLICKDGVVLAADTRASAGFF
                     IADRHVMKIQKVDRHLAMTIAGGVADAQNLVDVMRYNSNLYRLQNKELMPIKSAARLC
                     SNVLFNQRFFPYYVQIIMAGWKKEEGGQIYNIDLFGSMTTEKFISTGSGSPVAYGYLE
                     SEYKDDMSVNDAYKVAMQAIAAAIRRNAGTGDSINVVIIDNDGYRALTKEEKAEERVV
                     F"
     gene            complement(1478..2938)
                     /locus_tag="VE308_00290"
     CDS             complement(1478..2938)
                     /locus_tag="VE308_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZA99438.1"
                     /translation="MNLKQWCQLDEQIYIAEMDERYKQHAGVSYSERMIERLAEMRNI
                     SAETFVNFFLQPRELFLSSLENIADSSTKKLELKLYNLRNDKIISSRYRFARAPVNWS
                     TWRQFNSIEKDPKKRKHVFDEFISKTRYISPVIKERFDQMDRIYRQYSDNKLSPLDGY
                     LENEKISYSHLEDLVKSMGRQARKPFQEALRAISKKVFGREAEYYDDFYFFRNRVYAD
                     LEKEFVGVNPIEQVRRTLATMQFDLSSIHIDTEQRKNKYPSPICFFVQVPNDIRVLYK
                     SESPYFDLQGCYHEMGHAAHASSISSQAEYWNRYSFSMGIAEIFSIFLERLTKNRKYL
                     SSLGIKKNNHILEEIEARNNFMDLFFVTFYTANSLMKAEFWHKKLSIEKASDVYARLI
                     KEYTGFETPGEYWMLHHILPDAIMYVPSYLIAAVRAVEFDHHLQDRFGENWWTQVETG
                     KYIREIIQPGAAINLSRFSRLDSSLFMNELIDIGSM"
     gene            complement(3217..>4930)
                     /locus_tag="VE308_00295"
     CDS             complement(3217..>4930)
                     /locus_tag="VE308_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZA99439.1"
                     /translation="SSMDLIQISNMSSKSVSARRTIRFTQSICPDCNMILDAEVFERE
                     GKVFMTKTCPTHGECEELYFGSYEMYKKFSTYWMDGKGAHAPNVMIDKCSCPNNCGLC
                     TNHLSHSGLSNMIITNRCDLTCWYCFFYVKKGLEGAYLYEPNMEQIRAMMKTLKAEKP
                     IAGNSIQVTGGEPMLRDDIIEIVNIMKEEGVDHIQLNTNGIKLAMSPETMRQVRMAGV
                     NNLYLSFDGVSPRTNPKNHWEIPYTLESARKCGMTVVFVPTVIKSINDHELGGIIGYA
                     QKNLDVVHAVNFQPVSLTGRMTKKEREKYRITIPDCIERIEEQTNGEISKDSWFPVPS
                     CMPMTNVIEAFSKKPKYELSIHFACGAGTYVFEDSDTKKLIPLTAFVDIKGVLEYFEE
                     KADEIKSGANRYWALLEVVRKLKQFVNKDKQPRGLDLARMFSSILLKRNFDSVGSWHV
                     RSLFLGMMHFQDKYNEDLERLQRCDIHYLTPDLRIIPFCAFNVIPEWYRDRIQKKYSI
                     PVEEWEKAHGQTLEAGLYRGLMRRGKPEAQMGCALSEMHKAAALEQADDHKGVELRNN
                     MAGA"
BASE COUNT         1328 a         1095 c         1044 g         1463 t
ORIGIN      
        1 gcagttttgg gcaagcgtct ggatttgtat cgacgatatc tgatctacaa ttggaagcga
       61 tctgtcgctt gtcgggacat tgtggtccat agtggcaaaa gtcagatcag gccgcctgac
      121 gcgtctttta ctcatccgaa gcccatcaaa ggcctgaggc gatgtcactt catgaataag
      181 atgccgatct acatataaca aagattgacc accctcctct gcctcttttt catatactat
      241 atgactgtcc cagatcttct caaatattgt atttggctgc aagctgctta gagaataagt
      301 atctcataag aggaatataa aataaacaaa cagacaggga gatagtttta aatcaggtcg
      361 cttcactcaa ttccataaat gtaccctcat gacaagagat atcctgatta tattgcagac
      421 cagataaaga aaggaaccac cacctgcggc ctaatatgca aggacggggt agtactggct
      481 gctgatacgc gcgcaagcgc aggcttcttt atcgccgaca ggcatgtgat gaaaattcaa
      541 aaggtcgacc gtcatctggc aatgactata gccggcggtg tagcagacgc acagaacctc
      601 gtcgatgtca tgcgttacaa ttccaacctc tataggctcc agaacaagga gctgatgcca
      661 atcaagtctg ctgcaaggct atgctcaaat gtgcttttca accagcgatt cttcccatac
      721 tatgtccaga taataatggc aggctggaaa aaagaagaag gtggacaaat ctacaatatc
      781 gatctgtttg gctcaatgac gacagagaag ttcatctcaa ctggcagtgg ctctcctgtt
      841 gcatacggct acctagaatc agagtacaag gatgatatga gcgtaaatga tgcttacaag
      901 gtagccatgc aagcgatagc agctgctatt agaagaaatg caggaacagg tgacagcatc
      961 aatgtcgtca ttatcgataa tgatggctac cgcgcgctga caaaggaaga aaaggccgaa
     1021 gaacgcgtag tcttctagtc ggcgcttacg atagccaatg cttgcgtttg agttagggtt
     1081 tacatactca aggataatcg catgtccaat gctatgacta tcacataatc aaattatcag
     1141 cagcagcatc agcatatatt tagttgcaaa aattattttc cactatcgct gtgggtagtt
     1201 atcgactgtt atagtataat aaattcatgc cgcaatgact gaattttttc ttcttgacca
     1261 cagtattttg ataagataac taatctagaa ttgtgagcta ggatcggata tatcttttgc
     1321 agtcattttg atcgcattat agatacttca tcaagatgat cttttgaacc atttgcttct
     1381 ttgcaatggt tgacttctta tggagtatag gtcttgttgc actatgataa gtattagatt
     1441 tccttgggag gatgagagtt agttatagac tgatatttta catactgcca atatctatta
     1501 gttcattcat gaacaggctg ctatcaagcc gagagaacct agataggttt attgcagcgc
     1561 caggctgtat tatttctcga atatatttcc ctgtttccac ttgcgtccac caattctctc
     1621 caaacctatc ctgcagatga tgatcaaact caacagcacg aactgcggca atcaagtagc
     1681 ttggcacata cattattgca tctggtaata tgtgatgtag catccaatat tcacccggtg
     1741 tttcaaagcc ggtatattcc tttatcagtc gcgcatatac gtcgcttgcc ttttctatcg
     1801 acagtttctt atgccaaaac tcagccttca ttagcgaatt ggccgtgtag aaggtgacaa
     1861 agaacagatc catgaagttg ttccttgcct caatctcttc aagaatgtgg ttgttcttct
     1921 taattccaag cgatgacaaa tatttcctat tctttgtcag ccgctctagg aatattgaga
     1981 atatctcggc tattcccatc gaaaaactgt agcgattcca gtattctgcc tgtgagctta
     2041 ttgaagaggc gtgtgctgca tgtcccattt catggtagca tccctgcaga tcaaaatagg
     2101 ggctttcgct tttgtacaac acccggatat cgtttggcac ctgtacaaag aagcatatag
     2161 gcgatgggta tttattcttc cgctgttccg tatcgatgtg aattgaagac aggtcaaatt
     2221 gcattgttgc aagagtgcgt ctgacttgct ctataggatt tacaccgaca aactctttct
     2281 caagatcggc atatacccta ttgcggaaaa aatagaaatc atcataatat tctgcttccc
     2341 tcccaaacac ctttttcgag atagctcgaa gcgcttcttg aaagggtttt cttgcctgtc
     2401 tccccataga cttcacaaga tcttccaaat gagagtaaga gatcttttcg ttctcgaggt
     2461 agccgtctaa aggagacaat ttgttgtccg agtattggcg ataaattcta tccatctggt
     2521 caaaccgctc cttgataaca ggtgaaatgt atcttgtctt tgaaataaat tcatcaaata
     2581 catgcttgcg ctttttcgga tccttctcaa tactgttgaa ctgacgccat gtgctccagt
     2641 taactggagc tctggcaaac ctgtagcgcg aagaaataat cttatcattg cgtaaattgt
     2701 acagcttgag ctcaagcttt ttcgttgacg aatctgcaat gttttcgagc gagctaagaa
     2761 acaactctct tggctgcaaa aagaagttga caaacgtctc tgcactaata ttcctcattt
     2821 ctgcaagtcg ctctatcatg cgttcgctat aggagacacc tgcatgttgc ttatatcgct
     2881 cgtccatttc tgcaatgtag atctgctcgt cgagctggca ccattgcttt aggttcatat
     2941 ggcttgggca tggagcacat ccaattttaa gtttcaaaat acaacagcaa tatttacatc
     3001 ctgccgcagt aagtaaacgc aacacccata actgacagca tatgcaggta ataggataat
     3061 taggtaggct gacaaaagat tgttggagtt aaagttgcat caaaaagatt tttcttctat
     3121 caaaagatct gagcacaata aaagcagaac tgcaaacatg aatgtagctt gaacgactat
     3181 ctatctatgc taaaagaaga aaaaagatgg gtgtgcttac gcaccagcca tattattacg
     3241 cagttctact cctttatgat catcggcttg ctctaaagca gcggccttgt gcatctctga
     3301 cagagcgcaa cccatttgag cttctggctt accccttctc attaagcctc gatataggcc
     3361 tgcttccaag gtctgaccgt gagccttctc ccattcttca actgggatgc tatacttctt
     3421 ctggattcta tcgcggtacc attctgggat gacattgaat gcacagaatg ggatgattct
     3481 aaggtctgga gtaaggtaat ggatgtcaca cctctggagc ctttcaagat cctcgttgta
     3541 tttgtcttgg aagtgcatca tgccaaggaa gagtgacctt acgtgccatg agcctacaga
     3601 gtcaaagttg cgcttgagca ggatgcttga gaacattctt gccagatcta ggcctcttgg
     3661 ctgcttgtct ttgttgacaa attgctttag ctttcggact acctcaagca gtgcccagta
     3721 cctattggca ccggacttga tctcgtcagc cttttcttca aagtattcaa ggactccctt
     3781 gatgtcaaca aatgccgtaa gaggtatcag tttttttgta tcgctgtctt caaatacgta
     3841 ggtacctgcg ccgcaggcaa agtgtatcga caattcatat ttaggcttct tgctgaacgc
     3901 ctcaatcaca tttgtcatgg gcatgcatga gggaactgga aaccacgagt cttttgaaat
     3961 ttcaccatta gtctgctctt cgatcctttc aatgcagtct ggaattgtaa ttctgtattt
     4021 ttcgcgctct ttctttgtca ttctgcctgt aagggataca ggctggaagt tgactgcgtg
     4081 aaccacatct aggttcttct gggcgtatcc gatgattcca ccaagctcgt ggtcattgat
     4141 cgacttgatg actgtcggga cgaatactac agtcattcca cattttcttg ccgattccag
     4201 tgtataggga atttcccagt gattcttggg gtttgtcctt ggacttacgc cgtcgaacga
     4261 aagataaagg ttgttgacac cggccattct aacctggcgc atagtttctg gcgacatggc
     4321 caatttgata ccgttggtgt taagttggat gtggtcgaca ccctcctcct tcattatatt
     4381 gacgatttca ataatgtcat cccgaagcat cggctcgccg cctgtgacct gaatagagtt
     4441 gcctgcaatt ggcttttcag ctttgagagt cttcatcata gcccttatct gctccatgtt
     4501 tggctcatag aggtaagcgc cttcaagacc cttcttgacg taaaagaagc agtaccagca
     4561 tgttagatca catctgttgg tgatgatcat gttagaaagc ccagaatgag aaaggtggtt
     4621 cgtgcagagc ccacagttgt tggggcatga gcacttgtca atcatgacat ttggcgcatg
     4681 ggcgccctta ccatccatcc aataggtact gaacttctta tacatttcat atgagccaaa
     4741 gtatagctct tcgcactcgc cgtgcgttgg gcatgtctta gtcataaaga ccttaccctc
     4801 tctttcaaag acctcagcat ccagaatcat attgcagtct ggacagatgc tttgtgtgaa
     4861 ccggattgtg cgtctagcgc tcacactttt gctagacatg ttcgaaattt ggattaagtc
     4921 catactactg
//
LOCUS       DASQTF010000013         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_558265,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000013 DASQTF010000000
VERSION     DASQTF010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558265"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VE308_00300"
     CDS             complement(<1..479)
                     /locus_tag="VE308_00300"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZA99440.1"
                     /translation="MSLTLLLSGLLLVSPQALTFIDPAELPREAAAQELREHILHNRP
                     SPSPNVNDPNLRVEEVFEGLQLPTSMAFLGENDILVTEKDSGTVQRIVDGELQEEPVI
                     DVQVANNDERGLLGIDISNTNGTGTANNEQYVFLYFTESGGGEDGDDWSAGIPPSGTR
                     "
     gene            1268..1486
                     /locus_tag="VE308_00305"
     CDS             1268..1486
                     /locus_tag="VE308_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LSm family protein"
                     /protein_id="HZA99441.1"
                     /translation="MAVKVLDESLGKVVLIKLKGGKVIRGNLHGFDQHMNLLLEDSVE
                     ILEEGKSNELGTIVVRGDNVVIISPPPK"
     gene            1531..1680
                     /gene="rpl37e"
                     /locus_tag="VE308_00310"
     CDS             1531..1680
                     /gene="rpl37e"
                     /locus_tag="VE308_00310"
                     /inference="COORDINATES: protein motif:HMM:NF003214.0"
                     /note="contains a zinc finger motif; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HZA99442.1"
                     /translation="MGKFTRKKTHIRCRRCGHNSYHKRGKRCAKCGYPDPRIRKYDWI
                     KRQVI"
     gene            1728..2336
                     /locus_tag="VE308_00315"
     CDS             1728..2336
                     /locus_tag="VE308_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99443.1"
                     /translation="MSTEIAFLAGSSIAGVISALFLGKLFRVRSNNKNPTRSSSSSSA
                     SIRAELLSLLFEKNLTAEAITRVYEAAQDGRIDRLERDKLLLKYKQQLDSLNQKVAYL
                     QPVADFSDVKELRNNLVSFLEDRISALDKKLLELSKSSASPSEDDIGAKAKKILDETS
                     KLQSAAPIEQKLFKAEEQNIEQLQKEIAQALQHLEQVEIDKD"
     gene            2523..>3095
                     /locus_tag="VE308_00320"
     CDS             2523..>3095
                     /locus_tag="VE308_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HZA99444.1"
                     /translation="MVARIIDGKVVSADVKSGVIGAVRELNAVGVQPCLATVLVGNDP
                     ASATYIESKHKDAKEVGIVTRDHRLAATIEQKELRELVQLLNNDPSVHGILVQLPLPP
                     HIDEFDILASLNPLKDVDGLTPYNVGMLQSGIAALKPCTPSGIMELLDYYKIDVAGMD
                     VVIVNRSNLVGKPLLILLLERDATVTICHSK"
BASE COUNT          946 a          626 c          668 g          855 t
ORIGIN      
        1 cgggtgccgc tgggtggaat gcctgcactc caatcatcac catcttcacc gccacctgat
       61 tctgtgaagt ataggaaaac atactgttca ttattagcag ttcctgtgcc attcgtgttg
      121 gagatgtcaa ttcctaacaa acctctttcg tcattgtttg caacttgaac atcaattaca
      181 ggctcctcct gtagttcgcc gtcaacaatt ctctgcactg taccagaatc tttttctgtg
      241 accaagatat cattctcacc tagaaaggcc attgaagtag gaagttgtag accttcaaag
      301 acctcttcta ctcggagatt gggatcattt acgttaggtg atggagaagg tcgattgtgc
      361 aaaatatgct ctcgcagttc ctgtgctgca gcttccctcg gaagttctgc agggtcaata
      421 aaagtaagag cctgtggaga tactaacagt agacctgaga gcagtagtgt aagggacatc
      481 gtaatacttt ttggtaaata cattattctc actacaagga gtcgtagtat actggcttaa
      541 taacaattag gatttccccc tctggtaaat ttgacgtaag gtgcactatg aaaattaact
      601 actaatttta ggatagagtt tgattccaag gttactatat caagagggta ggatatgctg
      661 attctatgat taagagttat agaaaatatt tattatagtg tctatgtaaa aggaacagat
      721 agcaacattc ttttggcgcc acgccaaaaa catgcctaca ttattgaggt aaaataatga
      781 tctctataaa ttagtgatat agaactgaat agcgatctat agcattatga tctccaaggt
      841 gatgatacaa caagaaaaat tactcatgtg gctacccaaa gcctggatgc atataaacta
      901 gccatgaagt attataaccg aatatcattc ttatgctata gtcaaccaat tactggtggt
      961 tgctctggtt tataagaaaa ggccacgaaa tgggcaacgt tcgtggtaaa gtcctaaatc
     1021 attcagatag cttcattgtt gcaatagctg ttattattat ctaaccaaaa aatgtaatat
     1081 actcgagttc tagaagctcg atacaaattg tataggtata cccttgtatc ctcctaagtt
     1141 gttgttcatt gataactgtc caatgcttgg cttggcttac ataagttaac agaagataaa
     1201 ctaaacaaat tttaatatat gcctatatca tgctctaccc tcggtagaat actaattgtc
     1261 cgtagatatg gcagtaaaag ttctagatga gagtcttgga aaagttgtcc ttatcaagct
     1321 taagggtggc aaagtcatca gaggaaacct tcacggtttc gatcagcata tgaacctgtt
     1381 gcttgaagat tcggtagaaa ttctggaaga aggcaagtct aatgagctgg gcaccatcgt
     1441 tgtcagagga gacaatgtgg tcattatctc accaccgcca aagtaatgta aggagaatct
     1501 gagtgcgaaa tgacaaaagg cactacatcg atgggcaagt tcactcgtaa aaagacccat
     1561 atacgatgca gaaggtgtgg ccacaactca tatcataagc gaggtaagag gtgcgcaaaa
     1621 tgcggctacc ccgacccaag aattcgcaag tacgattgga ttaagcgaca ggtcatctga
     1681 tacaacagaa agcttttctt ctagcaagcc ttggcaaact ccatagaatg tccacagaaa
     1741 tcgccttcct cgctggctca tctatcgcag gcgtaatatc agcattattt ctgggcaagc
     1801 tttttcgcgt cagaagtaac aacaaaaatc ctacacgatc gtcatcatca tccagtgcct
     1861 ccatacgtgc agaacttcta tcacttctat ttgagaagaa tcttacagct gaagcaatta
     1921 ctcgggtata tgaagcagct caggatggca ggatcgacag gctagagcgt gacaaattgt
     1981 tgctgaaata caagcagcaa ctcgattcgc ttaaccaaaa ggtcgcttac ctacagcctg
     2041 tggcagactt ttcggatgtc aaggaactcc gcaacaacct tgtatcgttc ctcgaggaca
     2101 ggatttctgc actcgacaaa aagttattag agctttctaa atccagtgct tctccttctg
     2161 aagatgatat tggcgcaaag gcaaaaaaaa tactcgacga aacatcgaag ctgcaatctg
     2221 ctgctcctat agagcaaaag ttgttcaagg ccgaagaaca aaacattgag cagttacaaa
     2281 aggagatagc tcaagcgcta caacaccttg agcaggttga gatagacaag gactgataga
     2341 tttagcgtat aaatacttca ggcgaggctt gaaatagtgc aagtggcata gtatgtttct
     2401 atgttctttt tgctatctta ttctatttgg gtttatagag attatactat attgcatact
     2461 atcattccgt gaagtatcat ttatagaaaa gcaaatagtg tagaaaattc tcacctattc
     2521 aattggtagc gcgtataatc gatggcaagg tcgtgtcggc agatgtaaaa tcaggagtaa
     2581 taggggcggt tcgagaattg aacgcagttg gcgttcagcc gtgtcttgcg actgtgctag
     2641 tcggaaatga tcctgcatct gcaacctata ttgagagcaa acataaggat gcaaaagagg
     2701 taggtattgt cacaagggat cataggcttg ccgccaccat tgagcagaag gagctgcgcg
     2761 agctcgtgca actcctaaac aacgatcctt cagtccacgg cattttagta caattacccc
     2821 tgccccccca tatcgatgaa ttcgatatac tagcttccct aaatccgttg aaggatgtgg
     2881 atggcttgac gccgtacaac gttggcatgc tgcagagtgg tatcgcagca ctcaagcctt
     2941 gtacgccttc tggtatcatg gagcttttgg attattacaa aatagatgta gccggaatgg
     3001 acgtagtcat tgtaaaccgc agtaacttgg tggggaaacc gcttttgatt ttgttgttgg
     3061 aacgagacgc caccgtgaca atttgccatt caaag
//
LOCUS       DASQTF010000014         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_558307,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000014 DASQTF010000000
VERSION     DASQTF010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558307"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VE308_00325"
     CDS             <1..141
                     /locus_tag="VE308_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HZA99445.1"
                     /translation="QPHSEIAALAIFLDRIFTGKQFKRVFADANLKIVPTEKGKKVQE
                     NK"
     gene            complement(294..962)
                     /locus_tag="VE308_00330"
     CDS             complement(294..962)
                     /locus_tag="VE308_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99446.1"
                     /translation="MVVEEILYDFGEFPGYADDFVRNLLNLMIISKMNSAIKNLTSKN
                     YFMHLISQIDGCEAYVVKYGQPLLYAKYHGMEFTDQKVTTQFVRSKDHMISVTMESVF
                     GEFVKSFDSLASMTKSKVKWGIMKEKEDKSDPLFALLDSFVGAVIRLSSLDPSNPDSL
                     VGKRFGIRNASIIRKSLHLEFLVNSNLNIMELNPHKKHKDDAIKLLFGRPEVAKAIVS
                     LMKQ"
     gene            1127..>2614
                     /locus_tag="VE308_00335"
     CDS             1127..>2614
                     /locus_tag="VE308_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZA99447.1"
                     /translation="MSRSNIGRFMKKHGISNWCQLIEKANSNIEWYWNAVNEDLGIEW
                     FQKYDKTYDSRAGIPWTKWFLNGKCNIVVNAIDRHAKKQPDKIAYIFANEQGSRKITY
                     RELDEQVSRLADALATAGVRKGDVIAIYLPMIPEAFYAIFACAKIGAVHTTIFSGFSA
                     HALHSRLVDSKAKLLITTDAARRRGKEIDLASQWQKAVQCTNVSRIVTVGGNYDDLVK
                     NAGMAKTEVMDSEDPLFILYTSGTTGHPKGTLQVHGGFTIVTAQQAAYLIDMNPEDVL
                     FWYADIGWITGQVWVVYGSSIIGGTALVYDDALDYPTTDTWCRLIEDHKVSIFGAAPT
                     AIRLFMKSNVQTSRYDFQSLRVLAATGEPINSEAWTWYFRNVGKRRCPLVNLSGGTEI
                     GGAILSVLPVMPLKPCTVGCPVPGFDADVFDDAGKRASQGYLVIKKPWPSMTRGLFDG
                     PSRFLDTYWSKYKDVWYHGDLVFVNSDGLWYMQGRADDIIKVAGHR"
BASE COUNT          737 a          566 c          621 g          690 t
ORIGIN      
        1 caacctcact ctgaaattgc tgcattggca atttttctag acaggatatt tacaggtaag
       61 caatttaaaa gggtgtttgc tgacgccaat ttgaaaatag tgccgactga aaaaggaaaa
      121 aaagtgcagg agaacaagta atgtaataat cttttttctt ccgccatgtc agatgataag
      181 agagagttat aggacactga ccgggcgctc gcctcttcag cgagatgaag aaagaatcat
      241 gacgacacag agacatattg aacaagtgta ttgtcttggt atatacataa tcgctattgt
      301 ttcattaggc tcactattgc tttggcgacc tctggcctgc caaagagtag ttttatcgca
      361 tcatccttgt gctttttgtg ggggttcagc tccatgatgt tcagattgct gtttaccaga
      421 aattcaaggt gaagcgactt tcttatgatg cttgcatttc ttatgccaaa gcgcttaccg
      481 acaagactgt cagggtttga agggtcaagc gacgatagcc ggataactgc tcctacaaat
      541 gaatcgagca gtgcaaagag tggatcggat ttgtcctctt tttccttcat aatgccccat
      601 ttaactttgc tctttgtcat tgaggccagg ctatcaaagc ttttgacaaa ttcgccaaac
      661 accgattcca tagtaacact gatcatatga tcttttgacc taacaaattg cgtcgttacc
      721 ttttggtcag taaattccat tccatggtac ttggcgtaca gcagcggctg gccgtatttt
      781 acaacatagg cttcgcaccc atctatttgc gatataagat gcatgaagta gtttttggat
      841 gtcagattct tgatagctga gttcatcttg gagattatca tgagattgag aagattcctg
      901 acaaaatcgt cggcgtaacc gggaaactcc ccaaaatcat aaagaatctc ctccacgacc
      961 aagacagtaa atatcttata cccagcacat tagaattttt tcttacacta tgtgccacag
     1021 acgcggtaca attgtcaaca ctgccgtcta gttgcaaata ttaaacgtct cgtcaaatgc
     1081 cggcttattg actgagcggt tcatattcgt accttcagca gaccagatgt ctaggagtaa
     1141 tattggcaga ttcatgaaaa agcacggcat atccaactgg tgtcagctga ttgagaaggc
     1201 aaacagcaat atcgagtggt attggaatgc ggtcaacgaa gaccttggca tcgagtggtt
     1261 ccaaaaatat gataagacct acgactcacg ggcaggcatc ccttggacaa aatggttctt
     1321 aaatggcaaa tgcaacattg ttgtcaatgc aattgacagg cacgccaaaa agcagccaga
     1381 taagatagca tacatttttg caaatgagca aggttcaagg aaaatcactt accgcgagct
     1441 tgacgagcaa gtgagcaggc tagcagatgc gctagcaaca gctggtgtca gaaaaggcga
     1501 tgtcattgca atctatctcc ctatgatccc agaggcgttt tatgcgatct ttgcatgcgc
     1561 caagatcggg gctgtccaca ctacaatctt ttccgggttt agcgcacatg cactccactc
     1621 caggcttgtt gattccaagg caaagttgct tatcaccacc gacgcggcca ggcggagagg
     1681 taaggagatc gatcttgcaa gccagtggca aaaggcagta caatgcacaa atgtttcccg
     1741 aatagtgacg gtcggcggca attatgacga ccttgtcaaa aatgctggca tggccaagac
     1801 agaagtgatg gattcagaag acccgctgtt tatcctatat acatcgggta ctacaggcca
     1861 tccgaagggc acgctgcagg tgcatggcgg ttttaccatt gttacagccc agcaggcagc
     1921 atatcttatc gatatgaatc ctgaagatgt tttattctgg tatgcagaca ttggctggat
     1981 aacagggcag gtatgggtcg tatacggaag ctcgataata ggtgggactg cccttgtata
     2041 tgacgatgcc cttgactacc ccacaacaga tacatggtgc aggctgattg aagaccacaa
     2101 ggttagcatc tttggagctg cccctactgc aattaggctc tttatgaaaa gtaatgtgca
     2161 gactagcaga tacgattttc agtcacttag agttttggct gcaactgggg agccaataaa
     2221 ctccgaagcc tggacttggt attttagaaa tgtaggaaaa agacgctgtc ccttggtcaa
     2281 cttatcaggg ggaacagaga tcggaggcgc aatactgagt gtgctgccgg tcatgccgct
     2341 taagccctgc acagtaggat gtcccgtccc tggctttgat gccgatgttt ttgatgatgc
     2401 tggcaagaga gcaagtcaag gatatcttgt aatcaaaaag ccatggccct ccatgacacg
     2461 tgggctcttc gacggcccca gcagattcct agatacttac tggtcaaagt acaaggatgt
     2521 atggtatcac ggtgacctag tgtttgtcaa ttctgatggc ctatggtaca tgcaaggccg
     2581 cgccgacgat attataaagg tggcaggtca tagg
//
LOCUS       DASQTF010000015         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_558314,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000015 DASQTF010000000
VERSION     DASQTF010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558314"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VE308_00340"
     CDS             <1..268
                     /locus_tag="VE308_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HZA99448.1"
                     /translation="GPHDGFGTADWDLQRMKAAERAMIMRNQGFIHPATLVPDELEYN
                     KGYEARMSKLQSKFKDLEEIMNGKSKAATKAAATKQAKSGSSKL"
     gene            265..927
                     /gene="tpiA"
                     /locus_tag="VE308_00345"
     CDS             265..927
                     /gene="tpiA"
                     /locus_tag="VE308_00345"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZA99449.1"
                     /translation="MKRPLIINFKNYEEVSADRAIKLAESASQVAEKLKIEIVVAPPQ
                     PVLALIAKSVQVPVICQHVDNERIGPSTGFIVPEIAKSYGAVGSLINHSEHRMEMSAV
                     ASLVERMRKLDMASIVCAQEPQEVIEISSLQPDFIAIEPPELIGSGRAVSKENPSIIT
                     KSVQGAGSRSSIICGAGITDKDDVAKAMELGSQGILVASGVVKATSWEKKIAELASGM
                     KG"
     gene            complement(954..1886)
                     /gene="lipA"
                     /locus_tag="VE308_00350"
                     /pseudo
     CDS             complement(954..1886)
                     /gene="lipA"
                     /locus_tag="VE308_00350"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            1961..>2892
                     /gene="gcvT"
                     /locus_tag="VE308_00355"
     CDS             1961..>2892
                     /gene="gcvT"
                     /locus_tag="VE308_00355"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZA99450.1"
                     /translation="MQTPFYDIHKSLGAKIVNFHGWEMPLQYSGIMDEHVNVRANAGL
                     FDVSHMGRFEIKGQHSLACLQKLVTNDVEKLADHQALYSPMCYEDGGIVDDIITYRLA
                     DDRFLVVVNSSNRSKDFEWISKYSKSAHVEDISDRTGLLAIQGPRACDMLQNLVETNL
                     RSVKPFHLVDANIDGIQCMLSRTGYTGEDGFEIFFDSSRIEIWDKLMQYAESFGIKPT
                     GLGARDTLRLEAGLMLYGNDMDENTTPLEVPLKWTISFDKKQEFIGKKALINSHPSRK
                     LIGFEVLEKRIARQGNKVLMKGMEVGFVTSGSFSP"
BASE COUNT          814 a          636 c          679 g          763 t
ORIGIN      
        1 cggtccacat gacggctttg gcactgctga ctgggattta cagaggatga aggcagctga
       61 gcgtgccatg ataatgcgta accaaggctt tatccatcca gccacacttg ttccagacga
      121 gctagaatac aacaaaggct atgaggctag aatgtcaaag ctgcaaagca agttcaaaga
      181 tctagaggag ataatgaatg gcaaaagcaa ggcagcaaca aaggctgctg ctacaaaaca
      241 ggctaaatct ggcagcagca agctttgaag cgtccactaa ttatcaattt caaaaactat
      301 gaagaggtct ctgcagatag ggccatcaaa ttggccgaat cagcgagtca ggttgcagag
      361 aagctaaaaa tagaaatagt agttgcgcca ccccagcctg tgctggctct gattgcaaag
      421 agcgtacagg taccggtgat atgtcaacac gtagacaacg aaaggatcgg ccccagtacc
      481 ggttttattg tacccgagat cgccaagtca tatggtgcgg tcgggtcgct gataaatcac
      541 agcgagcatc gaatggaaat gagcgcagtt gcgagcctag tggagcggat gagaaagctt
      601 gatatggcat caatcgtgtg cgcacaggaa cctcaggaag ttattgagat atctagcctt
      661 cagcctgact ttatcgcaat agagccgcca gagcttatcg gcagcggaag agcggtgtca
      721 aaggaaaatc cttcaataat aaccaagtca gtacaaggag caggatcacg atcaagtatc
      781 atatgcggag caggtatcac tgacaaggac gacgtcgcta aggcaatgga gcttggctct
      841 caaggcatac ttgttgcaag cggagtagtc aaggccactt cttgggaaaa aaagatagcg
      901 gagcttgctt cgggcatgaa aggttaatca gccgcagaag gctatccatc ttttcaatct
      961 tgtttaagat gagatctgct actgctacta atgctctgct ctcgtctgta aaccatgttt
     1021 ttgatgaaca attctcctgc cttgtaggag cttcggacga atggacctgc caccacgtag
     1081 ccaaatccca tcttttcaat gcttttccta tatgaatcaa atttttctgg tgtgatatac
     1141 tctttaactg gcaggtgtct gctggtgggc tggaggtatt ggccgatgga aattatgtcg
     1201 actcctgcag accttaagtc cttggctgcc tgcataactt ccgcttccat ctctccaaga
     1261 ccaagcatta tggaagactt tgtatagatt ttgtaatcta tgtcttttat tatttctaga
     1321 acacgcaatg actgctcgta ggttgctcgt ggatctctga tcgacgcaga aaggcgggat
     1381 atagtttcaa tattgtggct tatgacttcc ggggccgcat ctaccacctt ttctattgca
     1441 tgacgattgc catgaaaatc cgggataaga ggctcaataa ttgtttcagg acattgagcc
     1501 ttgacagcct ttattgtttt tgcgatatga ttcgctcctc cgtcttgcag atcgtctctg
     1561 catactgaag tgatcacaac atatctcaat ccccactttt ttatcgcttt agcaactctc
     1621 tcaggctcat cggggtcaag atacagtggc ctgccaccgg ccacagagca gaacctacac
     1681 cctcttgtgc atatatctcc catgatcata atggtggctg ttcctcctcc ccagcattcg
     1741 ccaatgttag ggcatcgtgc ttcttcgcaa actgtgtgaa gccccagcga agcaatggtc
     1801 tgcctgatgc ttacgtattt ttccccagaa ggcaacctga tgcgaagcca ctcgggcttt
     1861 ttttctaatc tacccgtcat cattttgtcc cctttttgtt cgtcagcctc ttttaatcag
     1921 aacaatatgc tcaataataa ggattcatct tagatgaata ttgcaaactc ctttctatga
     1981 cattcacaag tcgcttggtg caaagatcgt gaattttcat ggttgggaga tgccacttca
     2041 gtactctggg atcatggacg agcatgtcaa tgtcagggca aatgccggct tatttgatgt
     2101 ctcgcatatg ggccgattcg aaattaaagg tcagcactct ctcgcctgcc tacaaaagct
     2161 agtaacaaat gacgttgaaa agctggcgga tcatcaagca ctgtattctc cgatgtgcta
     2221 cgaagacggg ggcatagttg acgacataat tacctacagg cttgccgatg atcgctttct
     2281 agttgttgtc aattcctcaa acaggagcaa ggatttcgaa tggatttcaa agtattcaaa
     2341 atcagcacac gttgaggaca taagcgatag aactggcctg cttgcgatac agggtccgcg
     2401 ggcttgtgat atgctgcaga atttggtaga aactaacctg cgatctgtta aaccatttca
     2461 tcttgtcgat gcaaatattg atggaataca atgcatgtta tctaggacag gttatacagg
     2521 agaagacgga tttgaaatat tctttgattc ttcaagaata gaaatctggg acaaattgat
     2581 gcagtatgcc gaatcatttg gaatcaagcc tacgggttta ggcgcacgag atacgctacg
     2641 gcttgaagcc ggcctaatgc tctatggaaa cgacatggac gaaaatacca cgccactaga
     2701 agttccatta aaatggacta tcagtttcga caagaagcaa gaattcatag gcaaaaaagc
     2761 gttaatcaat agccatccat caagaaagct cattggtttt gaagttctgg aaaagagaat
     2821 agcaaggcag ggcaacaaag ttctgatgaa aggaatggaa gtgggctttg tgacaagtgg
     2881 tagtttttcg cc
//
LOCUS       DASQTF010000016         4880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_3066,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000016 DASQTF010000000
VERSION     DASQTF010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1465
                     /locus_tag="VE308_00360"
     CDS             11..1465
                     /locus_tag="VE308_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZA99451.1"
                     /translation="MKACDTPSLHSWTKRITTSMTSHNSALGSKKFVYFPLDKEGQEQ
                     RQDSQGQSELTRDMLDKLLKAIGREALKEKTPSLQELEQTLQRVMSSLSNQQESSSQQ
                     EQSSGSLTDSRDESGDDSGMPFVAYLMKKGYLKDSPKWLSAKGFTAIGGKILSDVMKA
                     LKAGEYGTHESQNFGSGSLVLDTTKKYEPGDDIRLLNVPKSLLNTIQRTVKDGGSLKL
                     PLQIDVDDFEEYETIQDVRVAVVYCIDLSSTMRYSSMYGDMSRIEAAKRALWSLYLLN
                     RNFFPSDSVYIVGFGALASKVAPHDIPYLKTFEPGSDFLHYTNYQAAFRLANKILQKD
                     GAINKRLVLVTDGHPSACFIDDKSEQDRIVSQRPYSHFYTPDRGTIDVVKHNNSMNLD
                     IASGELVYLCYRYRQVDQYIGDRTILEAKKVHSKNIEIDTIMISEEDSLLGYVNEMEK
                     YVKGRSYYINPADIDKVLLTDYLNNKRQKTIMAH"
     gene            1540..2205
                     /gene="serB"
                     /locus_tag="VE308_00365"
     CDS             1540..2205
                     /gene="serB"
                     /locus_tag="VE308_00365"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HZA99452.1"
                     /translation="MLIIFDVEGVLLNAEYLPVLAQVMGPRKEKEIWDITNAGIRGEI
                     NWEEGLRRRVLALRGISYEDAKEIGEGLPIMPGAKELCSALKKAEWKMIAVSGGFTII
                     TDRLKEELGIDRIFSNELVFKDGKLEDVIMNVTSDKAAAIRPTIRQWDIRKEDTVVVV
                     DGANDLKLFALAGYTVGFCPVDMVREKADVSIDKRDLSLLLNLLKKNFGEAVLINAAS
                     RMH"
     gene            2242..2994
                     /gene="cofE"
                     /locus_tag="VE308_00370"
     CDS             2242..2994
                     /gene="cofE"
                     /locus_tag="VE308_00370"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZA99453.1"
                     /translation="MQLIQITPIRISDDIRLYNDLGAIILEAIDRSQIGMQNGDILVV
                     THKIVSKAEGRVVDLASIKPSIKAISMAKEHDKDPRVMELILKESSQILRAKNGIIIS
                     ETKHGFVCANAGVDQSNVEGDTAVLLPIAPDQSAGRLQETVKKKIGKEIAVIITDTFG
                     RTFRNGQTNVAIGVAGINPIKSYIGTYDMYGRKLKVTEIAVADEIASAAELVMGKAEG
                     TPVAIVRGYNFEKSSNTSIKSLLRAKERDLFR"
     gene            3046..3282
                     /locus_tag="VE308_00375"
     CDS             3046..3282
                     /locus_tag="VE308_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZA99454.1"
                     /translation="MNASPTKSIDVRGLYCPEPVFRTKMEVERLKIGDTIKVVADDPE
                     SEEDISRWVNRNGHELISLNKNDKDLEFVIKKAK"
     gene            3289..4287
                     /locus_tag="VE308_00380"
     CDS             3289..4287
                     /locus_tag="VE308_00380"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HZA99455.1"
                     /translation="MSAVARPDVVEQIGQTPLIELRSFSTENVKLYAKLEWHNPFGSV
                     KDRAAYWMIKHAEKKGILKKDKSIIIEPSSGNTGIALAGIAAALGYKVEIVIPEKVSE
                     ETKNILRNLGATLHETSDDLCPRVGAGTDQSIALAKAISKPRPDIYYMPNQYDNDTNF
                     LSHYESTGPEIWKQTNGTVTHFLTGCGTGGTITGTATFLKEKNKNVHAVAIQAQKNHL
                     LQGLRNFEESAIPELFKRRLSVVDEWMTATNKDSFESVRKLVEKETMLVGPSSGSVMT
                     SMLKVAHNLDHGIIVGIFADDGKKFRSLYVRENVLTAQEYDRALKSAKYMSKLAYS"
     gene            complement(4478..>4880)
                     /locus_tag="VE308_00385"
     CDS             complement(4478..>4880)
                     /locus_tag="VE308_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZA99456.1"
                     /translation="DSLRASKILQQKALENPKIEFIWNNVVADIKGNNKISTVLVKDI
                     NTGNERTIETGGLFVAIGHEPNTAIFKGQLELDDKGYIVLKQHTRTSVEGVFAAGDVH
                     DHRYRQAVTAAGFGCMAAIDVEKWLSERKHS"
BASE COUNT         1502 a         1022 c         1188 g         1168 t
ORIGIN      
        1 gaggtgatac ttgaaggcct gcgacacacc cagcctccac tcttggacaa aaaggataac
       61 aacatctatg acctcgcata atagtgccct cggcagcaag aaattcgtat actttccact
      121 cgataaagag ggtcaggagc agaggcagga cagccaaggt cagagtgagc ttactcgcga
      181 tatgctagac aaactactca aggcaattgg aagagaagca ctgaaggaaa agacgcctag
      241 cttgcaggag cttgagcaga ctcttcagcg tgtaatgtca tcactttcaa atcaacagga
      301 aagtagtagc caacaggagc agagctcggg atcgttgacc gattcaaggg acgaaagtgg
      361 agacgattct ggcatgccct ttgttgcgta tctgatgaaa aagggctatc taaaggattc
      421 tccaaagtgg cttagcgcta agggctttac tgcaataggc ggcaagatac ttagtgacgt
      481 aatgaaggcc ctcaaggccg gcgaatatgg cacgcacgaa tcacagaact ttggatcggg
      541 atcacttgtg ctggacacta ctaaaaagta tgagccgggt gatgacatta ggttgcttaa
      601 tgttccaaag tcgctgctga acacaatcca acgtacggta aaggatggtg gcagtctaaa
      661 gctgccactt caaattgatg tagatgactt tgaagaatac gaaacaatac aggatgtccg
      721 tgtcgcagta gtatactgta tcgacctcag ctccactatg cggtattcat caatgtatgg
      781 agacatgagc agaattgagg ccgcgaagcg tgcactttgg agcctctatt tgctgaaccg
      841 taatttcttt ccctcagact cggtctatat tgtcgggttt ggcgcgctag catccaaggt
      901 ggcaccgcat gacatacctt atctaaagac ctttgagccc ggctctgact ttttgcacta
      961 cactaactac caggctgcat tcaggctggc aaacaaaata ttgcaaaagg acggcgccat
     1021 taacaaacgc ctagtacttg tgacggatgg tcaccccagc gcgtgcttta tagatgataa
     1081 aagcgagcag gacagaatag tgtcacagag accttattct cacttttaca caccagatcg
     1141 ggggaccatt gacgttgtca agcataataa tagtatgaat cttgacattg catcaggaga
     1201 gcttgtgtac ctttgctacc gctatagaca agttgaccaa tatattggcg accgtaccat
     1261 tttggaggca aaaaaagtcc actcaaaaaa tattgaaatt gatactataa tgatcagcga
     1321 ggaggattct ttgcttgggt atgtcaatga aatggaaaag tatgtcaagg ggcgatcata
     1381 ctacattaac cctgcggaca ttgacaaagt actgttgaca gactacctta acaacaagag
     1441 acaaaagacg ataatggcgc attagcagga ctaatagaaa aaaaatatgt ggagaagctt
     1501 aatatactga cgggaatttg ttttgcataa gcaaatacta tgctgattat ctttgatgtg
     1561 gagggtgttc ttcttaacgc tgagtaccta ccagttttag ctcaggtcat gggtcccaga
     1621 aaagagaaag agatctggga catcactaac gccggcatca gaggcgagat caactgggaa
     1681 gaggggctca ggcgccgagt actagcactg agggggatta gctacgaaga cgccaaggag
     1741 ataggcgaag gtctgccaat aatgcctggg gcaaaagagc tttgctctgc gctcaagaag
     1801 gcggaatgga aaatgattgc agtatccggc ggcttcacaa taatcacaga caggctgaaa
     1861 gaagagcttg gaatagacag gatattctct aacgaattag tgttcaagga tggcaaactg
     1921 gaggatgtga taatgaatgt cacgtccgac aaggcagctg caattaggcc cacgataaga
     1981 caatgggaca tccggaagga ggatactgta gtggtggtag acggtgccaa tgacttgaag
     2041 ctattcgctc tggccggcta tacggtagga ttctgccctg tagacatggt gagagaaaag
     2101 gctgatgttt ccatcgacaa gcgtgatcta agcctactcc tgaacctgtt aaaaaaaaat
     2161 tttggtgagg cagtattgat aaatgcagca agcaggatgc attagcaacc aactgatttt
     2221 ttaacttcta tctttcaatc agtgcagttg atacagataa cccctattcg aatttcagat
     2281 gacattaggc tgtacaacga tcttggcgcg attatccttg aagcaataga tcgtagccaa
     2341 atagggatgc agaatggtga tattcttgtt gtcacgcaca agatcgtatc aaaagcagaa
     2401 gggcgtgttg tagaccttgc cagtattaag ccttcaataa aggcgattag catggcaaag
     2461 gagcacgata aagatccacg cgtaatggag ctgatattaa aggaatcatc gcaaattttg
     2521 cgtgcaaaaa atggcataat catatcagaa acaaagcacg gctttgtatg tgcaaatgct
     2581 ggcgtggacc aaagcaatgt ggaaggtgat actgcagtgc tgttgccaat tgcccccgac
     2641 cagtctgcag gcaggctcca ggagacggta aagaaaaaga tcggtaagga aatagcagtg
     2701 atcataaccg atacctttgg ccggacgttt agaaatggcc aaacaaatgt tgcaataggc
     2761 gtagctggaa taaacccgat taagagttac atcggaacat atgacatgta tggcagaaaa
     2821 ttgaaagtaa ctgagattgc ggtggcagac gagatagctt ctgcggccga gcttgtaatg
     2881 ggtaaggctg aaggcacgcc agtagccata gtccgaggct acaactttga aaaatcatca
     2941 aatacgtcaa tcaagtctct actgagagca aaggagcgag accttttccg gtagcaactg
     3001 taacatttaa tattacgaca ctataatttt acctgatttg ctactatgaa tgcctcacct
     3061 accaagtcaa ttgacgtaag gggactctac tgccctgagc cagtattccg cacgaagatg
     3121 gaggtcgagc gcctcaagat aggtgataca atcaaggtag ttgcagatga ccctgagtct
     3181 gaagaggaca tttcaagatg ggtcaatcgc aatggacatg agctgatatc gttaaataag
     3241 aatgataaag atcttgaatt cgtgataaag aaggcgaaat agagaaaaat gtcagcagtt
     3301 gcaagacctg acgttgtcga acaaataggg cagacaccgc tgattgagct caggtcattt
     3361 tctactgaga atgtcaagct atatgccaaa ctagagtggc ataacccgtt tggctctgta
     3421 aaggataggg ctgcatactg gatgataaag catgcagaaa agaagggaat cttgaagaag
     3481 gacaagagca tcatcatcga gccttcatcc ggtaacactg gtattgcgct ggcaggtata
     3541 gcagctgcac ttggctacaa agtagaaatc gttatacctg aaaaggtgag cgaggaaaca
     3601 aaaaacatcc tacgcaatct gggcgcgact cttcatgaaa catctgacga tctgtgccca
     3661 cgagtaggcg ctggcacgga ccagagcatt gcgcttgcaa aggccatttc aaagccaagg
     3721 cctgacattt actacatgcc aaaccagtat gataatgaca ccaatttctt gtctcactat
     3781 gagagcactg gccctgagat ctggaagcag acaaatggga ccgtcactca ctttcttact
     3841 ggctgcggaa caggaggtac aataactggt actgcaacgt ttctgaagga aaaaaacaag
     3901 aacgttcacg ccgttgcgat acaggcgcaa aagaaccatc ttctgcaggg attgcgaaac
     3961 tttgaagaat cggcaattcc tgagcttttc aagcgacgcc tcagcgtcgt agacgagtgg
     4021 atgactgcaa ctaacaagga ttcatttgaa tccgtaagga agcttgtcga aaaggagacc
     4081 atgcttgttg gaccttcgtc aggatccgtt atgacttcaa tgttaaaggt ggcacataac
     4141 ctagatcatg gtattatcgt tggcattttc gcagacgacg gcaaaaaatt taggagcctg
     4201 tacgtcaggg aaaatgttct aaccgcgcag gaatatgaca gggctcttaa gagtgcaaaa
     4261 tatatgtcca aactggctta ctcctgaact gaaggccccg tcagcttaga cgtgtgtaca
     4321 ataattcagt agaatgctgt acagaactca cagaactcaa aatggagtaa taccaatgaa
     4381 ggcatcctgt ctgggcattg caagctactt agactcataa gccatctggc acaattttac
     4441 actcaagaaa aaaagcctac agaattatta tagtttctca gctatgcttt ctttccgaaa
     4501 gccacttttc gacatcaatt gctgccatgc agccaaaacc tgcagcagtt acggcctgtc
     4561 tgtatctatg atcatgaaca tctccagcgg caaacacgcc ttcaacgctt gtcctcgtat
     4621 gttgcttgag gacgatgtaa cccttatcgt caagctcgag ttgtcccttg aaaattgcag
     4681 tattgggctc gtggccaatt gccacaaaga ggccacctgt ctcgatagtt ctttcattgc
     4741 ccgtattgat gtccttgacc aagacagttg aaatcttatt gttgcctttg atgtctgcta
     4801 caacattatt ccaaatgaat tcgatcttag ggttttcaag cgccttttgc tgaaggatct
     4861 tgctagcgcg cagagagtcg
//
LOCUS       DASQTF010000017         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1021264,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000017 DASQTF010000000
VERSION     DASQTF010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021264"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VE308_00390"
     CDS             <1..233
                     /locus_tag="VE308_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99457.1"
                     /translation="SSTNLTGFSVYTKENEDHPVGRVEDVDETGNLLIIRGSIDSKEY
                     SVPKNTEINVDKSSDKIVLYKTRDDFIKYAIV"
     gene            612..872
                     /locus_tag="VE308_00395"
     CDS             612..872
                     /locus_tag="VE308_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99458.1"
                     /translation="MASEVIATDRQYPKLSKAASGYLEIDHFKNADLHTGYFCYNCTY
                     FIKDNHCAIVEDTGPDVNGRESGIIAPHGLCTLWDPNEEEAR"
     gene            874..1245
                     /locus_tag="VE308_00400"
     CDS             874..1245
                     /locus_tag="VE308_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99459.1"
                     /translation="MTDIGKKIKEAAGAVDNLTKFKKRSKLQASYIEYDKFADSNLHE
                     GYFCYNCIYWMSASGGKCMLVEDKGPDVMGKVSDVIAPHGCCMAYEANYDKLHDTRSP
                     TEGVAIDTEKNKEKITTADVV"
     gene            1386..1874
                     /locus_tag="VE308_00405"
     CDS             1386..1874
                     /locus_tag="VE308_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99460.1"
                     /translation="MTEKLKDIKKTTSDAVEIIREIGSPDVQESLEKIRDTAKVGSEI
                     IKSLKEPAMVTNIENIRKTTESFEKTSARIERVTLELKNSGVLDETREIIKSAKNTIN
                     SVGDSKNISETMDAFKEMLRSISALVDELKLTVVESKGSGTIHNIEDAIRETRSAFME
                     HK"
     gene            2291..2554
                     /locus_tag="VE308_00410"
     CDS             2291..2554
                     /locus_tag="VE308_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZA99461.1"
                     /translation="MKYRSRTDITAQILEAANGGVTKTKIMYKAFLSYAQLKEYLTVL
                     IENGLLEYIEGEQIYRTTEKGNRFLKIYNQIGEYVAADVSEKK"
BASE COUNT          921 a          474 c          605 g          611 t
ORIGIN      
        1 catcatcgac caatcttaca ggtttttccg tatatactaa ggaaaacgaa gaccatcctg
       61 ttggaagagt cgaagatgtg gatgagactg gcaatttgct tattatacgc ggtagcattg
      121 attctaaaga atactcagtt cctaagaata ccgaaataaa cgtagataag agttctgaca
      181 agattgtgct ttataagaca agagatgatt tcatcaaata cgcaatagtc taggcggaat
      241 atggctggaa gttacaccat actttggcag tcgcgtttta attgcaaaac tctgcacagg
      301 cctataaaat ggcgctaatg atagcaagtt cagaagagct cagcacctca ccatgaggat
      361 ggatttcagg ctgctgacta ggagacaatt tcaaagcaag agcataaatt cgtttaatga
      421 gatgaaagaa aagtggaaag tcctcctctt tggatctggg cagtcatcgt gggtgcaata
      481 atacttgcca aattcacttt acgatggtca agactaaagc ggcttgcaag ggagctgaga
      541 aaaaggtaat aggacagaat cacagtgaga aataacaaac ttattttata ctatgccgca
      601 gcgataccgc tatggcaagc gaggttatag ctactgacag acagtatcca aagctctcaa
      661 aggcggcctc ggggtacctt gagatagacc acttcaaaaa tgctgacctg cacacaggat
      721 atttctgcta caattgcaca tacttcatta aggacaacca ttgcgctata gttgaagaca
      781 ccggacctga cgttaacggc cgagagtctg gcatcatagc accacacggt ctctgcacat
      841 tgtgggaccc aaatgaggag gaggcacgat aacatgacag acattggaaa gaagatcaaa
      901 gaagctgctg gcgcagtcga taatctgact aagttcaaga aaaggagtaa gctacaggcg
      961 tcatacattg agtacgacaa gtttgccgac tcgaacttgc atgaaggata cttctgctac
     1021 aactgtatct actggatgag tgctagtggc ggtaaatgca tgctggtaga agataaagga
     1081 cctgatgtga tgggcaaagt gtcagacgta attgcaccac acggatgttg catggcatac
     1141 gaagctaact atgacaagct gcacgataca cggagcccaa ctgaaggcgt tgcaatagat
     1201 acagaaaaga ataaggaaaa aatcacgact gcagatgtcg tgtaaccttt cttgttttag
     1261 ggcagatctt ataatcttaa taaggatagt gtgggtaaga tgctaaaaat tgagcaagtt
     1321 atagaacata cctatgtgaa acttttaccg ctttgcaatt aaagctgaaa gcgatactat
     1381 tgtaaatgac agaaaagctc aaagacataa aaaagacgac gtctgatgca gttgagataa
     1441 taagggaaat tggatcgcca gatgtacaag agtcgcttga gaagattcga gatactgcca
     1501 aggttggcag cgagataatt aaatcgctta aagagccagc catggtaact aatattgaaa
     1561 acattcgtaa gactaccgag tcatttgaaa aaactagtgc aaggattgaa agggtcacat
     1621 tggagctgaa gaattcaggc gtacttgacg agacaaggga aataatcaag tctgccaaaa
     1681 acactatcaa ctcagtaggc gacagtaaga acattagtga aacgatggat gccttcaagg
     1741 aaatgctgcg ctctatttct gcgcttgtag atgaactaaa actcactgtg gtggaatcga
     1801 aagggtcagg aaccattcac aacatagaag atgcaatacg agagacgagg agcgcattta
     1861 tggaacacaa atagagacgg tataagtcct gagtaatcgt caagtcgatc aagaacattg
     1921 ctggaaggac atatcagtga gcctgcccca caatgctcca aatggtcaag gtcatcgggc
     1981 agaatgcagg caatattgag gctccatggc ggtgcaataa aatgtacgac aatttgctta
     2041 aattgcaagt gagttgaagc agcgcacatc attagagact tagcacagtg gaagaaagaa
     2101 acatgtggtg ctgctataca taggacttat aagataatca gaaacaagat caagtaatta
     2161 acaggaagaa gaaccatgag gacaacaaaa agaaaggcga taataatatg atggtagttt
     2221 tattctagag taaagtttta tataataaaa cctcgagacg cttttataga tcctaatatc
     2281 taggatatcc atgaaatata gaagcagaac tgatataact gctcaaattc tagaagctgc
     2341 aaatggcggt gtcactaaaa ctaagattat gtataaagcc ttcctgtcat acgcacagct
     2401 caaggaatac ctgacagtgt tgatagagaa cgggctgcta gagtatatcg aaggagagca
     2461 gatctacagg acaactgaga aaggtaatcg cttcctaaag atatacaacc agattggcga
     2521 atatgtggca gctgacgtgt ctgaaaagaa gtagtaaaat actctatttt aaataaccaa
     2581 gatcactgaa tcagaatcgt gtctcaaaag c
//
LOCUS       DASQTF010000018         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_929190,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000018 DASQTF010000000
VERSION     DASQTF010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929190"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VE308_00415"
     CDS             <1..1104
                     /locus_tag="VE308_00415"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZA99462.1"
                     /translation="MAIKVAHASPKDKYNVLPCKSKLKSKSLRGIYDRNADSFQTLDA
                     ARMAADMLKSAKSYDRRVSVDSGNFSAQVATRALANSNGIELKERASAFSWSIMGMAI
                     EDTEISSFDYQVGSSHYVKDIDVYNTAREFAETAVNSLGTRKIEENFKGEMLLTPMAA
                     NEMIEEVVAHAINSDAVQKKSSHFAGKIGKHVASDLLTVEDDPTNVEGLNASSFDREG
                     VFHRRNLVIEKGVLKKFLYNTYTASKDGVRSTGNAGGSTSSPPTVSTTNFLIKPMRSS
                     VDTLVSEIDKGLIISRFSGNVDPVNGDFSGVVKGGRLVIAGTIKHAVKEVMVAGNIFE
                     ALRQLNGISRERKVIYDSILPYMRFDGVSFTTG"
     gene            1117..2331
                     /locus_tag="VE308_00420"
     CDS             1117..2331
                     /locus_tag="VE308_00420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZA99463.1"
                     /translation="MQEKEEKFDIAVVGGGPAGLSAAYSAAKGGAKVVLFEKDQSIAH
                     SIRTSGVTWISEMERLGIPSKFYNPIKNYRFVSPSNDVLITGNVSKSCVLDVRATYQH
                     LAFLAAKEGVQLMVKSNVTDVIRDGHKVAGVKASTPKGKLTVRSTLVIDASGFSTSVG
                     RKAGLAGQWKRYGVGAEYECYCDDIDSTTWVLMVGQKYSDAGYAWLFPLSTNRVRIGV
                     GIGRPESNAEPLEKLNRIIEKRFKPLDAVRDGKIQPLELHYGFIPNEGARQNSIADGL
                     LMVGDSAGQSNPLVLEGIRYAIDFGRLAGKVGADSLSQNSGRESLLEYERSWKAKAES
                     KIHSALKVQMRWLRLTDEEWDKEIEILREMTAHEFLDFIKADFTTANMMKLALHHPKV
                     AARQLFNMILKS"
BASE COUNT          796 a          519 c          652 g          609 t
ORIGIN      
        1 atggcgatca aggttgcaca cgcaagtcca aaggacaagt acaatgtcct tccatgtaag
       61 tccaagttaa agtcaaagtc actccgcggc atatacgata gaaatgccga ctcatttcag
      121 acattagatg ctgcaaggat ggcagctgac atgctcaaat ctgccaaatc gtatgacagg
      181 agagtaagcg ttgatagcgg taactttagt gcccaagtgg ccacacgcgc acttgcaaac
      241 tctaatggga tagagctaaa agaaagagca agtgcctttt cgtggtcaat catgggcatg
      301 gcaatagaag ataccgaaat ttcaagcttt gactaccagg ttggcagctc gcattatgtc
      361 aaagacattg atgtgtacaa tactgcaagg gagtttgcag agacagcagt caactcgctc
      421 ggcacacgta agatagagga aaacttcaag ggagaaatgc tgctaactcc gatggctgca
      481 aatgaaatga tagaggaagt agttgcacat gccattaatt cagacgctgt gcaaaaaaaa
      541 tccagccatt ttgcaggcaa gataggcaaa catgtggcgt ctgatctgct gacagtagaa
      601 gatgacccaa ctaacgtgga gggacttaat gcgtcaagct ttgacagaga aggtgttttc
      661 catcgtagaa atcttgtaat agaaaaaggc gttctcaaaa aatttctata taatacatac
      721 acagcaagca aagatggcgt aaggagcaca ggcaacgcag gcggatcgac aagctccccg
      781 ccaacggtat cgacaaccaa ttttctgata aagcctatga gatcaagcgt tgatacactg
      841 gtgtctgaaa tagataaggg tctaataata agcaggtttt ctggcaacgt agatccggtt
      901 aacggtgatt tttcaggtgt tgtaaaaggc ggccgactgg ttatagctgg gacgattaaa
      961 cacgcagtga aggaagttat ggtggctggc aacatctttg aggctctacg tcagctaaac
     1021 ggtatatcaa gagagcgaaa agtgatttat gactccatat tgccctatat gcgatttgat
     1081 ggcgtctcct ttactacagg atgagtaaaa aaaataatgc aagaaaaaga agaaaaattt
     1141 gatatcgcag tagttggcgg aggacctgca ggactttcag ctgcgtactc tgccgcaaag
     1201 gggggagcaa aagtagttct ctttgaaaag gaccagtcca ttgcacacag catcaggaca
     1261 agcggcgtaa cttggatttc tgaaatggag aggcttggca taccaagcaa gttttacaat
     1321 ccgataaaaa attaccgctt tgtatcgcct tccaatgatg tccttatcac aggcaacgtc
     1381 tctaaatcat gcgtgcttga cgttagagct acgtaccagc accttgcttt tcttgcggcg
     1441 aaggagggcg tgcaattgat ggtaaagagc aatgtcaccg atgttataag agacggccac
     1501 aaagtggccg gcgtcaaggc aagcacacca aagggaaagt taactgtgcg atcaacttta
     1561 gttatagatg caagcggctt tagcacgtct gttggcagaa aggctggtct ggccggccaa
     1621 tggaagcgct atggagtagg cgcagaatat gaatgctatt gcgacgatat agacagcacc
     1681 acgtgggttc tcatggtagg gcaaaagtat tcagatgcgg gatacgcatg gctatttcct
     1741 ctatcaacaa acagagtgag aattggagtg ggcattggca gaccggaatc aaatgctgag
     1801 ccacttgaaa agttgaacag gataattgaa aaaaggttca agccccttga tgcggtcagg
     1861 gatggtaaga tacaacccct agaactgcat tatggtttta taccaaatga aggcgcgagg
     1921 cagaatagca ttgctgatgg gctgctaatg gtcggcgact cggcgggcca atcaaatcca
     1981 ctggtgctgg aaggcatacg ctacgccatc gactttggga gacttgcggg aaaggtggga
     2041 gccgactcgc tttcgcagaa ctctggtaga gagtctttgc tggaatatga acgatcatgg
     2101 aaggccaagg cagagtccaa gatacactct gcccttaagg tgcagatgcg ctggcttcga
     2161 ttgaccgacg aggaatggga caaagagatt gaaattctcc gggaaatgac agcgcatgaa
     2221 tttttggact ttatcaaggc agactttacc acagccaaca tgatgaagct tgcactacat
     2281 catcccaagg tggcagcacg ccaattgttt aacatgattc tgaagagcta ataatctgaa
     2341 agtattgcaa caggatatcg tttgattttt gtacatggaa ctttaactca aaacgacgat
     2401 cgcttatttg tagatgctct aatatctctt catggatctt acgattatgc tatagatttc
     2461 atagaactga ggcattcaat gacagaggat ggaattattc tcttctaagg gccacaatag
     2521 gagatagcct tgacgccttc catgcgggat atatacccgc aataagactg agacca
//
LOCUS       DASQTF010000019         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_651361,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000019 DASQTF010000000
VERSION     DASQTF010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651361"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /gene="lysS"
                     /locus_tag="VE308_00425"
     CDS             <1..1212
                     /gene="lysS"
                     /locus_tag="VE308_00425"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZA99464.1"
                     /translation="SEAYKQGILAMQIDTILKNRSKIGQKIAEFVGQDKYTEVLPYFP
                     ICRSCGRLYTAVAQEYLPEERKVTYICRGGRIGKEEIKGCGNKGEADIARGEGKLAWK
                     VEFAARWLALDIRFEAYGKDIMDSVRVNDWIADEILGYPHPLHVKYEMFLDKAGKKIS
                     KSAGNVLTPQMWLQYGTPESILLLLFKRIAGTRHVGLDDVPALMDEYDYYEDVYFGKI
                     SEDNLAKLTKTKGIYEYINKLNPPKQPDPHIPYRFITQQASIFPPDDRDRDEKVFNRL
                     MKYGMVKGTKSDSIMYKIRLASNWGEDNMAKDEKFEIQLSDLHRKAVQELIEAIRPFT
                     GLPDSADNAKNLQSNVFSIARTNGIEPKEFFALLYRMFLNTDRGPRIGNYLLDLGIDR
                     ATSLLKRYLHG"
     gene            complement(1559..2335)
                     /locus_tag="VE308_00430"
     CDS             complement(1559..2335)
                     /locus_tag="VE308_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99465.1"
                     /translation="MSIAQSKRKLIAATGLFIALGALTGAIATGGAATISFAQTQEGG
                     GATAGQQGGGQERIVRQGTVTSAPEPTPGHEEHQYASILPLRQDGSIYSGVLTYTASV
                     PVEVVVLNMQTLNETEQAIVNGTEDDGFGTILTTPLDNQTSIAITVITPPYGDTPVPS
                     ASIPFAGNAIWLHTLDGTPFVANYAVTAQTLPAETMNNINNATVAVGEEADAAEDADA
                     DAAEDADADAAEDADADADAGEDEADGAANGNATAGADEA"
BASE COUNT          895 a          596 c          665 g          882 t
ORIGIN      
        1 agcgaagcgt acaaacaagg aattttggcc atgcaaattg atacaatatt gaagaacaga
       61 agcaagattg ggcaaaaaat agccgagttt gtaggtcagg acaagtatac tgaagtctta
      121 ccttattttc cgatatgtcg cagttgtggg agactctata ctgcagtagc acaagagtac
      181 cttccagagg aaagaaaagt tacttacata tgccgagggg gcaggatagg caaggaggaa
      241 atcaaaggct gtggcaacaa gggcgaggcg gacatagcaa ggggtgaggg gaagcttgca
      301 tggaaagttg aattcgcagc aaggtggctg gctcttgata tccgttttga ggcgtatggg
      361 aaggacatca tggattctgt cagggtaaat gattggattg cagatgagat acttggttat
      421 cctcatccac ttcatgtgaa gtacgagatg ttcttagaca aggcaggcaa aaaaataagc
      481 aaatctgcag gcaatgtact tacgcctcaa atgtggctgc agtatggaac gcctgaatca
      541 atactgctat tgctgttcaa gcgcattgca ggcacccgcc acgttgggct agatgacgtt
      601 cccgctctga tggacgaata cgattattat gaagatgttt acttcgggaa aataagtgag
      661 gacaacctcg ccaagcttac aaaaaccaag ggaatctatg aatatatcaa taagctcaac
      721 ccacccaagc aacccgaccc acacatacca tatcgcttta taacacaaca ggcgtcaata
      781 tttcctcctg acgatcgcga ccgcgacgaa aaggtattca accgcctcat gaaatatggc
      841 atggtcaagg gaaccaagag tgatagtatc atgtataaga ttagattggc atccaactgg
      901 ggagaagaca atatggcaaa ggatgaaaag tttgaaatac agctttccga tctgcatagg
      961 aaagcagtac aggaattgat cgaagcaatc agacctttta caggcctacc cgattctgca
     1021 gataatgcca agaaccttca atctaatgta ttttctattg cacggactaa tgggatcgag
     1081 ccgaaagaat tctttgcttt attgtataga atgttcctaa ataccgatag aggacctagg
     1141 ataggtaatt acttactaga cctaggaata gacagagcaa caagtctcct taagagatat
     1201 ctccacggct gatcagattt atcgggggac aattattttc tcattttgaa attatctttt
     1261 accgaatgtt tttttggcaa ttttttgact ggatcaagaa cattaataga atcagatacg
     1321 tggattaacc caatcaaacc tatctggttc aatttaatta tcggatctca ctttcgaaaa
     1381 gaaatactct gtttcccact ggaagtacat gttgctctaa attaacttgc attctagtac
     1441 agttattcta taaacataag gttagcattc gcagaacaag tataaacagt gagaaaaaag
     1501 gaaagaaaaa ggagggttta ttcaatttcc ctgtttgtct ttatcagttt gtattggttt
     1561 atgcttcgtc tgctcctgct gtagcgttcc cgttggctgc accgtcagct tcgtcttctc
     1621 cagcatcagc atcagcatca gcgtcttctg cagcatcagc atcagcgtct tctgcagcat
     1681 cagcatcagc gtcttctgca gcatcagctt cttctccgac tgctactgtg gcattgttta
     1741 tattattcat tgtctctgct ggtaaagtct gagcagttac agcataattt gcaacaaatg
     1801 gcgttccgtc tagtgtgtgg agccaaattg cattgcctgc aaatggaatt gatgctgatg
     1861 gaactggtgt atcgccatat ggtggagtaa taacagtgat tgctatgctt gtttggttgt
     1921 caagcggtgt cgtaagaatt gttccaaacc cgtcatcctc agtaccgttt actatagctt
     1981 gctcagtttc gttgagggtc tgcatattaa gaaccacaac ctctactggc acagaagctg
     2041 tataggtcag tactccgcta tagatagaac catcttgtct taatggcaaa atacttgcat
     2101 attgatgttc ttcatggccc ggtgttggct ctggtgctga agttactgtt ccctggcgga
     2161 ctattcgttc ttgtccacca ccttgttgtc ctgcagtggc gccgccgcct tcttgcgttt
     2221 gtgcaaaaga tattgtggct gctcctccag tagctattgc tcccgttagt gccccaaggg
     2281 ctataaatag acctgtagca gcgattaact tgcgtttgct ttgtgctatt gacattgaaa
     2341 gaatgaagtc ggtataacat ttattgattg tcaacatatg ttacgttgat aattacagat
     2401 tcttgtcata gagtttgctt cctctgttgg ttatctatat ccttccttgg atgctcaaaa
     2461 tctacaagat gctgaagaaa tgtgtcttct tcttgatctt tttgtgtaat ttttatatct
     2521 ttagcctttc tgtaaataaa attataacat tctgataatc acacgatttt ctaacaacca
     2581 cagtctggct gcattattag gtatgtaaaa tattcgtata acctaaaaac atctccatat
     2641 gaaatttcag ctggaaaaag tattgcaaaa tattggcaaa cgagatgaag gtggcctcta
     2701 tataattttt agaagcgcta taggttatag cttgcttgtg gctccccagg agaagtaaac
     2761 tagatgaaaa ggcttgcctc gggcgaaagg gctgcaaaca gtcaatgctt caacctctgt
     2821 tagtaatagc agtaccacgc aatatgcctc ttgcaagatt tcattctgat aagatataaa
     2881 atgaagtgca agatatgata aagaaaagat aagtgcattg acaaagatgt tagaggagaa
     2941 gaaggagcag agctgttgaa atcatgaagg taataatctt ctcgcatgaa tctgatcttg
     3001 atggcctcta ttctgctgca ataggactta tgcgctat
//
LOCUS       DASQTF010000020         6476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_466452,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000020 DASQTF010000000
VERSION     DASQTF010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466452"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VE308_00435"
     CDS             complement(<1..335)
                     /locus_tag="VE308_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99466.1"
                     /translation="MKTGERTGNNFRKGGRYLYADYTPLEQNIDFLSQLKDFSSLSDI
                     IFQAHNDFEHLRSVLANAESLESEIVSKIDQLSSMVGDIFGNFGIRYNEPLRERYSIP
                     TEADPFVIA"
     gene            complement(524..1009)
                     /locus_tag="VE308_00440"
     CDS             complement(524..1009)
                     /locus_tag="VE308_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99467.1"
                     /translation="MPGAVLKKGDFGSFMLQNIVELERRSGIKLGLAQKTLLAETGTI
                     EQVLSILTGSAVRVNIVQQRENEKTISRESVILNDADRVLIRAHSKIFVRNLPEKIAR
                     QIRQKQLGIGTIIANCHLETFRKIIEVGYDPINRSVFRRYQIIYREKVAFEIREELVG
                     V"
     gene            complement(1034..1744)
                     /locus_tag="VE308_00445"
     CDS             complement(1034..1744)
                     /locus_tag="VE308_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99468.1"
                     /translation="MSAENETRINEWFVPKFGPMRFRIFVGLLFLPYTGMCISFAIIG
                     SMLAGEIAWDRVGAIALIYILALGISAHVADSLGSKKTKPWGSYFTKVQLLTIMAATL
                     TAAYAIGVYYIVFYVPLLAVIAVLEGFFLFTYNYEIWDGIFHNDFWFALSWGSLPLLA
                     GCIIQTNSISILALTAAGLTGLASYAEIKMSRPYKQLKQRNGNAARARRLENGLKAIS
                     VGTITFALSMLAYRIIFS"
     gene            complement(1741..2409)
                     /locus_tag="VE308_00450"
     CDS             complement(1741..2409)
                     /locus_tag="VE308_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZA99469.1"
                     /translation="MLVHEGHAQAMRFFTRANADSYDLIVRLATFGQDIAWKHEITRA
                     VTHRSQILELACGTGILSSILAQRGKSVAGIDITFEYLLSLKRKLKAPIAQGTAEVLP
                     YRDGSFDAIVSSYLAKYVDVQKVIDECWRVLRPGGIVIFHDFTYPRGLIRNLWNIYFE
                     LLRLAGRFVTSWKVVFRQLDDVIKKSDWVDQTIGALKNKKFQNINCRCYTAGTSAMVS
                     AEKP"
     gene            2486..3190
                     /locus_tag="VE308_00455"
     CDS             2486..3190
                     /locus_tag="VE308_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99470.1"
                     /translation="MYFVNWQRKVSLEERNGQKVMVKRNKPTKQFHEFLIIYAYSLIS
                     MLLFHPSAPLTFSEIIRNEGYEMRKNLRKIGISTPMLLSMSDTDLIEEYIEGGDIYRA
                     LASGGDVMLAYKAGCITGKAHQAGYTFVDNKAQNYLAKGESVIRTDLGFMKKTNSHYS
                     KSMDIGSFLASVMDLDRYSEIAKSFYDGYKFEAGSKKFSYLSIVIRNVLSAGFSSNSK
                     TTLRNMMLDSRSLISL"
     gene            complement(3187..3582)
                     /locus_tag="VE308_00460"
     CDS             complement(3187..3582)
                     /locus_tag="VE308_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99471.1"
                     /translation="MDDFEKNFPGFDWKNTPAYKHPGGKDCPCPKHEYIREQINVAKT
                     MSENNKETSPEFRQKAQVTLKFCPDHYEVYFKEVIPSMPLKYRLMTKVAVRLGAISIQ
                     KLTYMQSDQCFYCKFGSGGFDKKAELPPI"
     gene            3664..4731
                     /locus_tag="VE308_00465"
     CDS             3664..4731
                     /locus_tag="VE308_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99472.1"
                     /translation="MLITYTLSLKMTKNIFLMLLHSVSDVVRDSDSGGGAILTATATN
                     SNSGNDDLDPNRKTLLEFKGAVQEEQKTGDKRIEEINNKLDSIKKQIDEERTQLDDFR
                     SKLKQVNGEKDIDYPKFVQLRESLMEAKNQMKSLDDKVGPVAAKLRKERSDMHNLQKA
                     LEQIERDIQTKKLSKDEERKLVARSKEIATKLHTLKMIHKKEDQYRTISAQYDQIKDR
                     VKRIFKLKEEYGTKIGKLKESLDNLINLREGLYEERRQVIHTVREAAAKLEMIETQLN
                     AIAFKKSRMQAAEYRHKRQKETGERRAARQEAMQERVKRDKEYQERRNALKEVALKKM
                     STGKKLTFDEMKLIFGDAGSD"
     gene            4772..5032
                     /locus_tag="VE308_00470"
     CDS             4772..5032
                     /locus_tag="VE308_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99473.1"
                     /translation="MADKIEYSGKVYVYSSGMPAGHIQLTKETLSEYGVKENDIEVIE
                     VPEGVPEGSVMVTVWPHYLSVARVKRVREGSIYAPQLFNIQL"
     gene            complement(5042..5203)
                     /locus_tag="VE308_00475"
     CDS             complement(5042..5203)
                     /locus_tag="VE308_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99474.1"
                     /translation="MAYTLAFDSLHVKRAIVIRPAFVAYWPIAGTVAVTITFTKECQH
                     PTRMGLQKI"
     gene            complement(5219..5770)
                     /gene="nuoB"
                     /locus_tag="VE308_00480"
     CDS             complement(5219..5770)
                     /gene="nuoB"
                     /locus_tag="VE308_00480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HZA99475.1"
                     /translation="MDELSSQEEKFPKLIDPSASFGAFIGKLGDVLIKAVDKPLGQAV
                     NWGRMYSLWPVHLETGCCSVELGAASSPRYDLERFGVLEAFGSLRQCDLIIVLGTVNR
                     KMAPRLRVVYDQMPEPKYVVAMGACAITGGLYFDSYNVLPGVDNILPADVYVPGCPPR
                     PETLIQAIMLLQEKIKRSSLKKV"
BASE COUNT         1874 a         1406 c         1431 g         1765 t
ORIGIN      
        1 cttgctatta caaatggatc agcttcggtt ggaatagaat atcgctctct taatggctca
       61 ttatacctga taccaaaatt gccaaagata tccccgacca tgctgcttaa ttgatctatc
      121 tttgagacta tttcagattc caaagattct gcgttagcga gtaccgatct tagatgttca
      181 aagtcgttgt gcgcttggaa aatgatatca gaaagactcg aaaagtcctt gagttgagat
      241 aagaaatcta tgttttgctc taatggagta taatcggcgt aaaggtatct tcctcctttt
      301 cgaaagttat tgcctgtacg ttctcctgtt ttcatgttat tgttttacta tcaactatgg
      361 tatccatttc aaggatataa atcaaatatt gaaatagcga attagggacg atagattact
      421 tgatgagaat tgatatataa taccacaaca ataaactcag cttctccggg atagtgatta
      481 acgtcatggg ttcaaattca ccaaaatcct actttctcct attttatacc cctaccaact
      541 cttcccttat ctcgaaagca accttttccc tgtaaatgat ctgatatctt ctgaaaactg
      601 acctgtttat tgggtcatat ccgacctcaa ttatcttcct gaatgtttca agatgacaat
      661 tagcgattat cgttccgatg cctaattgtt tctgtcttat ctggcgtgct atcttttcag
      721 gcagattacg gacaaaaatc ttgctgtgcg ctcttataag caccctgtct gcatcgttga
      781 gtattacaga ttctctagaa attgtctttt cgttttctcg ttgttgtaca atattgaccc
      841 ttactgcaga gcctgtcagt atactcagaa cctgctctat ggtacctgtt tctgccagca
      901 gagtcttttg agctagccca agcttgattc cagatcttcg ttcaagttca acaatatttt
      961 gcagcatgaa gcttccaaag tcgccttttt taagaactgc gcctggcaca atatgattga
     1021 tagaaaggaa gtgttatgaa aagattatcc tgtacgcgag catagagagg gcaaatgtta
     1081 ttgtaccaac actgattgcc ttgaggccgt tctcaagcct ccgggcccga gcagcgttgc
     1141 catttctctg ctttaattgc ttatagggtc gagacatctt gatctcggca tagctggcaa
     1201 gtcctgtcaa gcctgccgcc gttagtgcaa ggatgctgat cgaatttgtc tgtatgatgc
     1261 atcctgccaa tagcggcaaa gatccccatg acagtgcaaa ccaaaagtca ttgtggaata
     1321 tgccgtccca tatttcatag ttgtacgtaa ataaaaagaa cccttcaagc actgcaatga
     1381 ctgctaggag tggaacatag aatacaatgt aataaactcc gattgcatat gcagctgtaa
     1441 gtgttgctgc catgatagtc aacagctgca ccttagtaaa gtagctaccc cacggtttgg
     1501 ttttttttga cccaaggcta tccgcaacgt gagcggaaat tcccagcgca agtatgtaaa
     1561 tcaatgcaat cgccccaacc ctatcccatg ctatctcgcc agcaagcatt gaaccaataa
     1621 tagcaaatga aatacacatg ccggtgtagg gtagaaacag caagccaaca aagatgcgaa
     1681 atctcatagg gccaaatttg ggcacgaacc attcatttat gcgcgtctca ttctctgcac
     1741 tcatggcttt tcagcagaga ccatagcaga agtgccggcg gtgtagcacc tgcaattgat
     1801 gttctgaaat tttttattct taagggcacc tattgtctga tcaacccagt ctgatttttt
     1861 tatgacatca tccagctgcc taaaaacaac cttccatgat gtaacaaacc ttcctgctag
     1921 tctgagcaat tcaaaatata tattccatag attgcgtatg agccctctag gataagtaaa
     1981 atcatgaaat atcacaatac cacccggccg gagcactctc cagcattcgt cgattacctt
     2041 ttggacatcg acgtattttg ctaaatagga ggatactatc gcgtcaaatg atccatccct
     2101 gtagggcagg acttcggccg tgccctgcgc tataggcgcc ttcagcttgc gctttaagga
     2161 gaggaggtat tcaaacgtga tgtcaattcc ggccacgctt ttgcctctct gtgctagtat
     2221 tgacgagagg atgccggtgc cgcacgcaag ctccagaatc tggctacggt gggtgactgc
     2281 ccttgttatc tcatgtttcc aggcaatgtc ctgtccaaac gtcgccagcc gcacgattag
     2341 atcatagcta tctgcattgg cacgagtaaa gaagcgcatt gcctgagcat gaccctcgtg
     2401 aactagcact agcagtgcta ttacagtcgt cctactttta tgtattgaaa agtaaatcaa
     2461 tgcctttgag ccactcgaac tttgattgta ttttgtaaac tggcagagga aagtctcact
     2521 ggaggagcga aatggccaga aggtcatggt gaagagaaat aagcccacaa aacagtttca
     2581 cgagtttctc ataatctacg cttactcgct tatttcgatg ttgcttttcc acccgtctgc
     2641 tccacttacg tttagcgaaa taattagaaa tgaaggttat gagatgagaa agaacctaag
     2701 aaagatcgga atatctacgc cgatgctgct ttcgatgtct gatacggacc taatagagga
     2761 atatattgag ggaggagaca tctacagagc acttgcttct ggtggcgacg ttatgcttgc
     2821 atacaaggca gggtgcatta caggcaaagc tcatcaggca ggctatacct tcgtagacaa
     2881 caaggcacaa aactaccttg caaagggcga gtctgtcata agaactgacc taggctttat
     2941 gaaaaaaact aattcgcact actcaaaaag catggatata gggtcgttcc ttgcaagcgt
     3001 gatggacttg gacaggtaca gcgaaattgc caagtcgttt tatgatggct acaagtttga
     3061 ggcaggcagc aaaaagttct catatctatc aatagtcatt cgtaacgtgc tgtcagctgg
     3121 cttttcatca aacagcaaga caacgctcag gaacatgatg ttagactcca ggagcttgat
     3181 cagtctctag attggcggaa gctctgcttt cttgtcaaag ccacctgagc caaacttgca
     3241 gtaaaagcac tggtcagact gcatgtaggt tagcttttgt atcgatattg cccccaacct
     3301 gactgccacc tttgtcatga ggcgatactt caaaggcata gatggtatta cttctttgaa
     3361 gtaaacttca tagtggtcag gacagaactt aagcgtcacc tgagccttct ggcggaactc
     3421 tggcgaggtt tctttgttat tttcgctcat tgtcttggcg acattaatct gttctctaat
     3481 gtattcatgc ttgggacatg ggcagtcttt tccgcctgga tgcttgtagg caggggtatt
     3541 tttccagtca aagcctggaa aattcttttc aaaatcatcc atcgctggtg tagactacgt
     3601 attctactct tataaagatg taggacaatc tccacctgta tagaggaagc aatgctaaaa
     3661 tgaatgctga ttacgtatac actgagcctc aaaatgacaa aaaatatatt tttgatgtta
     3721 ttacactccg taagcgacgt ggtacgagac agcgacagtg gtggcggtgc tatattgaca
     3781 gcaacagcca caaatagcaa ttctggcaat gacgacttgg atccgaaccg caagactttg
     3841 ctggaattta aaggggctgt tcaagaagag caaaagacag gtgacaagag gatagaagag
     3901 ataaacaaca agcttgacag catcaagaag cagatagatg aagagaggac ccagcttgat
     3961 gattttcgct ccaagttaaa gcaggtcaac ggggaaaagg acattgacta tccaaagttt
     4021 gtacagctac gagagagcct catggaagcc aagaatcaga tgaagagcct agatgacaaa
     4081 gttggccctg tggcagctaa gttgcgaaag gagcgaagcg acatgcacaa ccttcagaaa
     4141 gcacttgaac agatagagag agacatccag actaagaaac tatccaagga cgaagagcgc
     4201 aaacttgttg cccggtcaaa ggagattgcc actaagctgc acacgctgaa aatgattcat
     4261 aagaaagaag atcagtatcg cactatttca gcacagtatg atcagataaa agatcgggtc
     4321 aaaaggatct tcaagctaaa agaggagtac ggtaccaaaa taggcaaact aaaggaaagc
     4381 ctcgacaacc tgataaacct ccgggaaggc ctttatgaag agcgcaggca ggtaattcac
     4441 acagtgagag aagctgctgc caaactagaa atgattgaga ctcaactgaa cgctattgct
     4501 ttcaaaaaat cgcgcatgca ggctgcagaa tatcggcata agaggcagaa ggagacggga
     4561 gagaggcgag cagcacggca ggaagcaatg caagaaagag taaagcgcga caaagagtac
     4621 caggagcggc gtaacgcctt gaaagaagtg gcgctcaaaa agatgagcac gggtaaaaaa
     4681 cttacatttg acgaaatgaa gttgatattt ggggatgcag gctctgactg agatacattt
     4741 ttttgataga cgattgtaac ctgccctgga gatggcagat aaaatcgagt acagcggcaa
     4801 ggtctacgta tatagcagtg gtatgccagc aggccatatt cagctcacaa aggaaacgct
     4861 gagcgaatat ggcgtgaagg aaaatgacat tgaagttatt gaggttccgg aaggtgtccc
     4921 tgaagggtct gtaatggtga cagtctggcc tcattatttg tcagttgcaa gagtcaagcg
     4981 agtgcgcgaa ggctcaatat acgcgcccca gttgtttaac atccagttat gatctaaaac
     5041 attaaatctt ctgtaaaccc attctagtag gatgctgaca ttcttttgta aacgtaatcg
     5101 tcacggcgac tgtcccggcg attggccagt aggcaacgaa tgcgggcctg atcacgattg
     5161 ctcgtttgac atgaagagag tcaaatgcca gtgtatatgc cataaaggaa actaatttct
     5221 acactttctt caaactactt cgctttatct tctcttgtaa gagcattatc gcctggatta
     5281 gtgtttcagg gcgaggagga caaccaggaa cataaacatc agctggtagt atattatcta
     5341 ctcctggcag aacattatag gagtcaaaat acaatcctcc tgtaatagca caagctccca
     5401 tagctaccac gtattttggt tcaggcattt ggtcatatac aacccttagg cgaggtgcca
     5461 tttttcgatt gactgtgcca agaactatta ttaagtcaca ctgcctgaga gagccaaaag
     5521 cttcaagaac gccaaagcgt tctaagtcat aacgaggact cgatgctgca ccaagctcta
     5581 cactgcagca tccagtctca aggtgaaccg gccataacga atacatcctc ccccaattta
     5641 ctgcttgacc gaggggtttg tcaactgcct ttatcaatac atcacctagc ttgcctataa
     5701 acgcgccaaa gcttgcgctg ggatcaatga gcttgggaaa tttctcttct tgagaagata
     5761 gctcatccat gtcagggttt ctgtgtgata ggctaaaaag attagagaga aaggtatcta
     5821 tgcgtgtctc ctgtactatc tttcaacctc tataggccag acgtccagag actctcctgt
     5881 gtatcatcgg cagttcagct gacttggtgt ctttaagcaa atactgcagg agaagcacaa
     5941 ggtatcttac ttatgcacac ttatttgtgg gggtatggtt tactggtcac catcactcaa
     6001 gatatatgtc ctagtgcacc tctctgcccg attcaatact gcaatatgtc ttcccaggcg
     6061 ttcccaagcg cccctcttgg gtttggtgga agctttgttc ttgtttacct tccttttggc
     6121 gttttggcca attactgccg aattatttta acgttctggc gtatatcata tgtgcattaa
     6181 gagataaatg cagtaattac tgtatcacaa gagggggcct aatatgatag tgcagtgcac
     6241 tttcatataa gatctctgcc cgccttaatg cattcttttt cctgatcggc gctcaaacct
     6301 gcgttagtat tgcaggtttt tattttagca ccgatataac gcaaccttta attgagcttg
     6361 tacacttttt cgatttggaa aaaggcaatc aaatgctagg cttctcttca tttctatccc
     6421 agcttaacgg tttgtttgga ggagaagggt tagatcctag cagcccatgg atgtgg
//
LOCUS       DASQTF010000021         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_4365,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000021 DASQTF010000000
VERSION     DASQTF010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4365"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VE308_00485"
     CDS             complement(<1..1022)
                     /locus_tag="VE308_00485"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZA99476.1"
                     /translation="MLETAKGSSAAINPFEVALKQLDEAAKLINLDKGLQKVLANPKR
                     VLTVSLPIRMDSGEIQVFTGFRSQHNDARGPYKGGIRYHPQVSIDEVKALSMWMTWKC
                     AIADIPYGGGKGGIICNPKEMSEGELERLTRRYAYSIADIIGPHTDIPAPDVYTGGKE
                     MAWIMDTYSALKGNYVQPEIITGKPIAIGGSLGRNEATGRGLAFTVREAAKKLKIDMK
                     DTTMAVEGFGNAGQFASQFVENQGAKLIAASDSRGGIYNKNGIDVAKLRKHKEKTGSV
                     VEFSGANSISNEELLETECTILIPAALENQITAKNAGRIKAKVVAEAANGPTTPEADE
                     VLYNNR"
     gene            complement(1087..1434)
                     /locus_tag="VE308_00490"
     CDS             complement(1087..1434)
                     /locus_tag="VE308_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZA99477.1"
                     /translation="MDVSSALVMNIAGSEWIIIILFGLILLFGTKKLPQFSRTVGKAV
                     GEYEKAKDMFRHEMEQAAENARGGSKFPKITGPVATERKKLESIASSLGIEDHEGLTD
                     EELRVLISKRMSS"
     gene            complement(1893..>2118)
                     /locus_tag="VE308_00495"
     CDS             complement(1893..>2118)
                     /locus_tag="VE308_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZA99478.1"
                     /translation="GYAIYNKSRKMGYAIWAFPVVIGITRIYVMQHYPTDIIAGFMLG
                     GLVSVVLSNAMKLDEPFFLSRIKGKEDRAF"
BASE COUNT          540 a          483 c          424 g          671 t
ORIGIN      
        1 atcctgttgt tgtagagcac ttcgtctgct tcaggtgtag ttgggccgtt ggcagcctct
       61 gccactacct tagccttgat tctgccagca ttcttggcag taatctgatt ttcaagcgca
      121 gctggtatta gtattgtgca ttctgtttca agtaactctt cattgcttat cgaattggcg
      181 ccagaaaact ctaccactga gcctgttttt tccttgtgct ttcttagctt ggcgacatca
      241 ataccattct tattataaat gcctcctctg gagtcagaag cagcgatcaa cttagcacct
      301 tgattttcta caaattgtga agcaaactga cctgcatttc caaagccttc caccgccatc
      361 gtggtgtctt tcatgtctat cttaagcttc tttgcagcct ctcttaccgt gaacgccagt
      421 cctctgcctg tggcttcgtt tctgccaagt gatccgccaa ttgcgatcgg tttgcctgtg
      481 ataatttcgg gctgaacgta attgccttta agtgcagagt atgtgtccat tatccaagcc
      541 atttccttgc cgccagtgta aacatctgga gctggtatgt ctgtgtgcgg accaataatg
      601 tctgcaatag aataagcata gcgccttgta aggcgttcta actcgccttc tgacatttcc
      661 tttgggttac atatgatgcc gcctttgcct ccgccgtatg gaatatctgc aatagcacac
      721 ttccaagtca tccacataga gagggccttg acctcatcga tggagacttg cggatgatag
      781 cgaatgcctc ccttgtacgg gccacgggca tcattatgct gcgagcgaaa tcccgtaaaa
      841 acttgtatct ctccactatc cattcttatc ggtagtgata ctgtaagaac gcgctttggg
      901 ttggctaaaa ctttctgtaa acctttgtct aggtttataa gcttggctgc ttcgtcaagc
      961 tgtttgaggg caacttcaaa gggatttatt gccgctgagg agccttttgc tgtctctagc
     1021 atgcattgtt ctagaaaagt tcatgttata agttttgtgt ataaattata gccagaatac
     1081 tgtaggtcag cttgacattc tctttgatat aaggacacgg agctcctcgt ctgtaaggcc
     1141 ttcatgatcc tctatgccta atgagctggc gattgactcc agctttttcc tctctgtggc
     1201 aactggacct gttatcttgg gaaatttgct acctcctctt gcattttctg ccgcctgttc
     1261 catttcatga cggaacatat cctttgcctt ttcgtattct cctaccgcct tgcccactgt
     1321 gcgtgaaaat tgaggtagtt ttttggtgcc aaacagtaga ataagcccaa aaagtatgat
     1381 tatgatccac tctgatccag cgatgttcat taccagagca gaagatacgt ccatcttgaa
     1441 tgatatttaa gattttgatt agaattaaag atttctacaa ctgaacagcc tttttatttg
     1501 gcaggatcct gagcagctgt gtgtctctat ctcgctatta gaaaaattgc aaaagatcaa
     1561 gccgaggtaa tacaggacaa aaagagatca cttctattca agagaaagaa ccctgaaaag
     1621 tagctttctc acagttgaag cataaagcat ataatttctc aatttctata aaaatttata
     1681 atataatagt agaaaaatga tatccaagac tcatagcact gccaccgtct atcctgtatc
     1741 tttttaattg gaatcaggca tgctcagaag cccacttctt atataattta tcatatcaaa
     1801 aaaattggag ttgtgcaaac ttgagcatat ttggtaatgc aacggcatga ttagcttcct
     1861 cctctgtaag cttgtgttgc agtatcaaat actcagaatg ctctatcttc cttgcccttt
     1921 attctagaca gaaagaatgg ctcatcgagc ttcatggcgt tgctcagaac cactgatact
     1981 aatcctccaa gcatgaagcc agcgataatg tctgtcgggt aatgctgcat cacatagatg
     2041 cgggtaatgc caataacgac tggaaaggcc cagatcgcat agcccatttt tcttgatttg
     2101 ttatagatag cataccct
//
LOCUS       DASQTF010000022         3545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_652592,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000022 DASQTF010000000
VERSION     DASQTF010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652592"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VE308_00500"
     CDS             <1..302
                     /locus_tag="VE308_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA99479.1"
                     /translation="RSEVMQMLVLKPNIAILDEPDSGLDIDSVKSVAEAINQLIDTGA
                     GVLVITHYARILRYLKKLDHVHVVARGKVIKSGGKELSEELEAKGYTWLGIEEDK"
     gene            335..1033
                     /locus_tag="VE308_00505"
     CDS             335..1033
                     /locus_tag="VE308_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HZA99480.1"
                     /translation="MASEASTSEEPPATTFLNFSFFRVDPKWRWLNEIGKEEAAKEFA
                     SLIEIANTKIKVRTYSTLGLREDSDLMLWMISNSLEKTQVMTSKIYSTILGKYLMPSH
                     VFLSATRPSVYSSKIKPGFMTDEQPMKYCIVYPFVKSREWYLLPFEERKKMMEEHILV
                     GRKFPNIRLNTTYSFGLDDQDFMLAFETADLMAFQELIIQLRETKVSKYIIRDTPMIV
                     CIYKGAEDIIKSLG"
     gene            1036..1590
                     /locus_tag="VE308_00510"
     CDS             1036..1590
                     /locus_tag="VE308_00510"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HZA99481.1"
                     /translation="MQALKEWAVVCRALEEGRQIVLLRKGGILEYRQGFKVKHDRFLL
                     FPTYEHQSKDHLQADYANKLDEVLEDQPSTGTNRITSYAESIEVKEITDRITLRMLAN
                     YHIWNESYVNARMDYNPSKPMSVILLRVFKLERPILIENKREWIGCRSWIPLDMDVSG
                     RAIMDDLQFNTIASEVKGVLSIAA"
     gene            1587..2573
                     /locus_tag="VE308_00515"
     CDS             1587..2573
                     /locus_tag="VE308_00515"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZA99482.1"
                     /translation="MKYRTLGKSGIKVSEIGFGAWSIALDWWGKKIDDDEAIRMLKRA
                     YDLGINFYETADIYGKGKSEKLIAQVFKDMRNDVVYSTKWGYDMYSAKQVGHSELPQK
                     HNQEFLQFAISHSLDRLQTDYIDVYSLHNPKMDAIVNEKLFASLDYMVKKGTIKSHGV
                     ALGPAIGWKDEGLEAIEKRNITCLQTVYNILEQDPARDFMQAAERRNVGIMVRVPDAS
                     GLLTGKVNADTKFDKNDHRSFRKQEFIIEAMQKIENMRPLAISKGWNITELAIKFILS
                     QKQISVVLPTMISIEEIELFTSLSDGKYLNNTEIAKMEEMYEKNFYVEPVAK"
     gene            complement(2697..>3545)
                     /locus_tag="VE308_00520"
     CDS             complement(2697..>3545)
                     /locus_tag="VE308_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZA99483.1"
                     /translation="MKNMGIIRQINAIFDTRRLGYKSALIAFAVRPDKLDYVAGKVNE
                     HPGVSHNYERNHEYNMWFTLAVPPGSDMKQDLDRMASFEGVIKHRVLPTLKLYKIGVR
                     LDMVNKDPDKLMPTDNVKHLNQEKVQLSERDREFIRELQKDLAVVPEPFKELANSLDV
                     TTAELFAKAIEYENTGIMRRFAAILRHREAGFVANGMVVWQVPENKVDEVGSRLAAFP
                     QVSHCYRRPVYPDWRFNLFSMVHARTLQAAEKIAAEMSETVGVKDYQILFSSREFKKE
                     RVKYFV"
BASE COUNT         1050 a          760 c          819 g          916 t
ORIGIN      
        1 aacgatcaga agtcatgcaa atgctggttc tcaaaccgaa tatcgcgatt ctagatgagc
       61 cggactcggg tctagacatc gattcggtca agtcagtcgc ggaggcaata aaccaattaa
      121 ttgatacggg tgccggcgtg cttgtaatca cccattatgc cagaatcctt cgatacctca
      181 aaaaacttga tcatgtccat gtagtggcaa ggggcaaggt tatcaaatca ggcggcaaag
      241 aactgtctga agaactggaa gcgaagggtt acacctggct cggcatagaa gaagacaagt
      301 aaaaattagg ccccagtaaa ttacttccac ccagatggct tcagaagcat cgacatcaga
      361 agagccccct gcaacaacct ttctcaactt ctcattcttc agggtggatc ccaagtggcg
      421 ctggctaaat gagataggca aagaggaagc agccaaagag ttcgcgagcc taattgagat
      481 tgccaacacc aagataaagg tgaggacata ctcaactctg ggcctacgcg aagatagcga
      541 cttgatgctc tggatgattt ctaactctct agaaaagacg caggtaatga catccaagat
      601 ctattcaact atccttggca aataccttat gccatcacat gttttcctct ctgcaacaag
      661 accgtctgtt tactcatcta agattaagcc aggatttatg acagacgagc agcctatgaa
      721 gtattgtatc gtctatcctt ttgtcaagtc aagggaatgg tacttgctgc cttttgaaga
      781 gcgcaagaaa atgatggaag agcatatctt ggtgggacgc aagtttccaa atatcaggct
      841 caacaccact tactcatttg gccttgatga tcaggatttc atgctcgcct ttgaaactgc
      901 ggatctgatg gcttttcagg aactgataat tcagctgcgc gaaaccaaag tgagcaagta
      961 cattatccgt gacactccaa tgattgtctg catttataag ggagctgagg acatcattaa
     1021 aagtctagga taattatgca ggcgctgaaa gaatgggctg tcgtctgcag ggctcttgaa
     1081 gagggcaggc agattgtgct tttacgcaag ggtggcatat tggaataccg ccagggcttc
     1141 aaagtcaagc acgacagatt tctattgttc ccaacgtacg aacaccagtc aaaggatcat
     1201 ttgcaggctg actatgcaaa taaacttgac gaagtgctag aggatcagcc gtccaccgga
     1261 accaatagga ttacaagcta tgcagaatcc atagaagtca aagaaataac agaccgcatt
     1321 actcttagga tgcttgcaaa ctaccatatc tggaacgaaa gctatgttaa tgcaaggatg
     1381 gattacaatc caagtaagcc tatgagtgta atactcttac gtgttttcaa actggagagg
     1441 ccgatattga ttgaaaacaa gcgggaatgg attggctgca gatcatggat cccacttgac
     1501 atggatgtaa gcggcagagc cataatggat gatctgcaat tcaacacaat agcgtctgaa
     1561 gtaaagggag tgctatctat agcggcatga aataccgaac tttgggtaag agtggtataa
     1621 aggtaagtga gattggcttt ggtgcatgga gtatagcgct tgactggtgg ggaaaaaaga
     1681 ttgacgatga tgaagctatc aggatgctaa agcgcgccta cgatttgggt atcaattttt
     1741 acgagactgc cgacatatac ggcaagggaa aaagcgagaa gcttatagcg caagtgttca
     1801 aagatatgcg caacgacgtg gtctattcca caaagtgggg ctatgacatg tatagcgcca
     1861 agcaggtggg acatagtgaa ctgccgcaaa aacataatca agaatttttg cagtttgcta
     1921 ttagccatag cctcgaccgg ctgcagactg attacataga tgtctatagc ctccacaatc
     1981 cgaagatgga cgcaatagta aacgaaaagc tcttcgcttc tcttgattat atggtgaaaa
     2041 aaggtactat caagagccat ggggtggcac ttggaccagc aatagggtgg aaggatgaag
     2101 gcctagaagc cattgaaaaa aggaacataa catgcctcca gactgtctac aatattttgg
     2161 agcaggatcc tgcacgagac tttatgcaag cagcagagcg acgcaatgtc ggcataatgg
     2221 taagagttcc tgatgcatca ggcttactta ctggcaaggt gaatgccgat acaaagtttg
     2281 ataagaacga ccaccgcagt tttagaaagc aagaattcat cattgaggca atgcaaaaga
     2341 tagagaatat gagacctctt gcaatcagca agggatggaa catcacagaa ctcgcgatta
     2401 agttcatact gtcgcaaaag caaatttcag tcgtcttgcc tacaatgata agtattgaag
     2461 aaatagaact gtttacatcg ctttcagatg gcaagtacct caataacact gaaattgcaa
     2521 aaatggagga gatgtatgaa aagaactttt atgttgagcc tgttgctaaa tgaaaccaga
     2581 ttgaggatct tcttcttctc ccgaccatat cttcatcata agattgttgc aggttgtaat
     2641 tgaaaaaaat gctatacagg tcatatagag tcgcttgtga gcgccgactg gatgagttat
     2701 acaaaatatt ttacgcgctc tttcttgaat tctcgtgagc taaacagtat ctgatagtcc
     2761 tttacgccca ccgtttctga catttctgca gcaatctttt cagctgcctg cagcgtccgg
     2821 gcatgtacca tgctgaaaag attgaacctc caatcagggt ataccggcct cctgtagcag
     2881 tgactcacct gtggaaatgc agcgagtctg gagcctactt cgtctacttt gttctccggc
     2941 acctgccaca cgaccatacc atttgcgaca aatccagctt cgcggtggcg aagtattgct
     3001 gcaaagcgtc tcattatgcc agtattttca tattctatgg ccttggcaaa caattccgct
     3061 gtagttacat ctaaactatt ggcaagctcc ttgaagggct ctggcactac tgccaagtcc
     3121 ttctgcaatt cacggatgaa ttctctgtca cgctcactta gttgcacttt ctcttggttt
     3181 agatgcttca cattatccgt tggcataagc ttgtccgggt ctttattaac catgtcaagc
     3241 ctgacgccga tcttgtagag cttgagtgtt ggcaaaactc tatgttttat aacgccttca
     3301 aatgatgcca ttctgtcaag gtcttgcttc atatctgatc cgggaggcac tgcaagcgtg
     3361 aaccacatat tatactcatg attgcgctca tagttgtggc tgaccccagg gtgctcattg
     3421 actttccctg ccacatagtc aagtttgtca ggtctgactg caaacgcaat aagagcgctc
     3481 ttgtatccaa gtcgccttgt atcaaaaatg gcattgatct gtcggatgat gcccatgttc
     3541 ttcat
//
LOCUS       DASQTF010000023         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_467376,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000023 DASQTF010000000
VERSION     DASQTF010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467376"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VE308_00525"
     CDS             <1..342
                     /locus_tag="VE308_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZA99484.1"
                     /translation="PHQDFIIEYIHRSVAATTGMLVIATMVFTLKSKSTPRGMKIASI
                     VAAGAVIGQITLGAIVIVERLHAVLVTAHLGLGLVLFSMVLMTVLYAFKIDNKRIKTS
                     TISSDLYPRKD"
     gene            371..1468
                     /locus_tag="VE308_00530"
     CDS             371..1468
                     /locus_tag="VE308_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99485.1"
                     /translation="MIYTDRGRYYHTVSMHRRLFIIAMAAIVILPLSSMVPAYAHFEH
                     FTHYNNRGGQLGPYYAYEALEPEYAGPNEPVAVMFSVQDRDGRDTYNIYSMVEIYSAT
                     TGERLEAWPWTRQEIGDFQVFYNFPEVGSYQIVLSVANDGNPVNPNSIDPSRSTLSSN
                     TGCNCDRIIFNVSITKNFGTIWNAMMLISISLPLSVFGGVLAWNYRRMIKGGRYHRSA
                     QRDTARYIVMLAAIAGGVVHLAVYAEHASLRLEYSIFLLVAAGMQIVYGVVYTLLTLT
                     GQGANESSRQYHKKKVAVNIFGLVGTGILLGLYAYSVIFPPPLSPNNQPESIGVAGIL
                     AKGIEVFTVVGIVYLMRLEKHELVGQLKEQT"
     gene            1557..>2018
                     /locus_tag="VE308_00535"
     CDS             1557..>2018
                     /locus_tag="VE308_00535"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZA99486.1"
                     /translation="MAWKVMMPKKIMFGENAAKEFPYPNNALVITTTTPDIYNKWMDY
                     MGIKGYEVYDKVAPDPAIETIEAIQKQYEGKDVSCYIGLGGGSSMDVCKYLSKLTGKP
                     KILIPTTFGTGAEMTTYAVISFSHKKKLLQDEAFLADAAIVDPYFLPGTPFN"
BASE COUNT          597 a          441 c          453 g          527 t
ORIGIN      
        1 cctcatcaag atttcatcat agaatacatc cataggtccg tcgccgctac gacgggtatg
       61 ctcgtaattg ctacaatggt ctttacgctg aaatcaaaga gtactcctag aggtatgaaa
      121 attgcttcca tagtagctgc tggggcagtg atagggcaga taactttagg ggcaatagtg
      181 atagttgaga ggcttcatgc agtattggtg actgctcacc ttggcctcgg gctcgtacta
      241 ttctcaatgg tgctcatgac agtgctctat gcgtttaaaa tagacaacaa aagaatcaag
      301 acatctacaa tatcatcaga tctctatccc cgcaaagact aataataaaa ataataataa
      361 ttgcagtgca atgatttata cggacagagg tagatattac cacacagtct cgatgcaccg
      421 acgactcttt attattgcaa tggccgcgat tgtaatatta ccactttcct ctatggttcc
      481 ggcatatgcc cattttgagc acttcaccca ttataacaac agaggaggcc aactgggccc
      541 gtactacgcg tacgaagcac tagagcctga atacgctggc ccaaatgagc ctgttgctgt
      601 catgtttagt gttcaggacc gtgatgggcg agacacatac aatatctata gtatggttga
      661 gatctattct gccacaacag gcgagcgcct tgaggcatgg ccttggacaa gacaggaaat
      721 aggtgacttt caggtctttt acaactttcc tgaagttggt agctatcaga tagtgctcag
      781 cgttgcaaat gatggcaatc ccgtcaaccc aaatagcatt gatccttcgc gatcaacact
      841 atctagtaat acgggctgca actgcgacag gattattttt aatgtatcaa tcaccaagaa
      901 ttttggcact atatggaatg cgatgatgtt aatctcaatc agcttgcctc tatcggtgtt
      961 tggtggagtg ttagcctgga attacagaag gatgataaaa ggcggcagat accacaggag
     1021 cgcgcagagg gacaccgcaa gatatatcgt aatgctagcc gcaatagctg gcggcgttgt
     1081 gcatctggca gtttatgcag agcatgcatc cctccgtctt gaatacagca tatttctttt
     1141 agttgcagcg ggtatgcaga tagtttatgg tgtcgtatat acgcttttga cattgactgg
     1201 acagggtgca aatgagtctt cgcgccaata ccacaaaaag aaagtagcgg tcaatatatt
     1261 tggacttgtt ggcacaggca tccttcttgg gctatatgcc tactctgtta tctttcctcc
     1321 tccgctttct ccaaacaacc agcccgaaag tattggcgtt gctgggatcc ttgccaaggg
     1381 gatcgaagtg tttacagttg ttggcatcgt ataccttatg cggcttgaaa agcatgaatt
     1441 agtcggccag ctaaaagagc aaacatagta agcaatcaac caataattat cttagcaaaa
     1501 gttattcatc ttatctagta agacataaaa caccatgagg ataaacccaa tagctaatgg
     1561 cttggaaagt aatgatgcca aagaagatca tgtttggcga aaacgctgca aaggagttcc
     1621 cctatccaaa taatgccctt gtcataacca caactactcc agacatttat aacaaatgga
     1681 tggattacat gggaatcaag ggctatgagg tctatgacaa ggtagcgcct gatccagcaa
     1741 tagaaactat tgaggcgata caaaaacagt atgagggcaa ggacgtctca tgctacatag
     1801 gcctaggcgg aggaagctca atggatgtct gcaaatatct cagcaagctg accggcaagc
     1861 ccaagatact gatccccacg acatttggaa caggtgcaga aatgacgaca tatgcagtca
     1921 ttagctttag ccacaagaaa aagctccttc aagatgaagc ttttcttgct gacgccgcaa
     1981 ttgtggatcc atactttttg cccggcacac cgttcaat
//
LOCUS       DASQTF010000024         4915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_652818,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000024 DASQTF010000000
VERSION     DASQTF010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652818"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VE308_00540"
     CDS             complement(<1..216)
                     /locus_tag="VE308_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99487.1"
                     /translation="MVRKFDDWWNTVPADLKQKARKGDESNKPLLNQINYVLLHLHLA
                     GKHDAKPSHDELKDWLHSGQVDVLRMNK"
     gene            322..1161
                     /locus_tag="VE308_00545"
     CDS             322..1161
                     /locus_tag="VE308_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99488.1"
                     /translation="MRKKSKLVPFAIHELHSAYRAYQQFLDCNPPLHHARYIFPFFRG
                     SYFAYRKVDVLRTVEIAKAISANPIYVDIGCGYGDFLDKVRQLLPDARGIERDGSIFY
                     AFEISKPDYISLMPAENLSEPVDVAFVGWMEQGQDFRRFVAKCAKCVITTFDTGGQCG
                     INSACEYEEFGFQRVAWWRTPSWIDVNGELMNRYYAPSLDSGKKEQLAKLRTAHNFWY
                     VYAKLELIDKIKSGLQGWLEKVENPNDRFDFESILDECGFHYMEELPALANNRKLWEV
                     KFD"
     gene            1162..1965
                     /locus_tag="VE308_00550"
     CDS             1162..1965
                     /locus_tag="VE308_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99489.1"
                     /translation="MPFNLEDLRKAKHELAQFGSNRGSPNHERYIFPFFKGSYFAYRK
                     VDVLRTVAVAKAISSNPKYLDVGCGYGDFLKQVRKFIPSAEGIEKNAGIFYGLGIIKP
                     DYISIADAHWIDKKYDLIFVGWMEPGQDFRDPIARSTDVIITTLDQGISLEAEFEGHG
                     FQRVAWWRTPSWEDVNIEIMNRYYTKIPDSKRTELAKMRGAHNLWYVYAKPQQVQSVR
                     AALKSQVEKESSFDYRYDFESVLDECGYGYLQKLESPAMLEPMWQVQWN"
     gene            2014..3276
                     /locus_tag="VE308_00555"
     CDS             2014..3276
                     /locus_tag="VE308_00555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZA99490.1"
                     /translation="MKVKVLGAAKEVGRSAFLVNCDHTNILMDYGVLLRREPIFPMHV
                     KPKEVDAVVITHAHLDHSGFVPSLYLSGSTNIPALGTLPTFELSELLIEDMIKLSGFY
                     LPFEYIDVMTMLKSSKNLQYREPYMVNNAKVTLHESGHVVGGASIVVEHEGKKIFYSG
                     DINTRGSKLLRPADLDFGEIDMMIIESTYSQSEQQPREEAEKELIEFAYEVIGRGGTL
                     FIPSFSVERAQEIATVLKAYNFKHKVVMDGMALKANQIMSRHPAFLKDAEMFRRAIGE
                     AEWIQGWSRRRRVVEEPCVIISPAGMLVGGSAIFYTQQIANSEKNGIAMVSYQGEGTP
                     GRMLLEKRVANFDGKIRKCYADVKRFEFSGHNSRSELFEILDRVKGNPKVLTVHGDGP
                     SCVKFAEEIKEKYGYDAHAPGQGEVIEV"
     gene            3273..3476
                     /locus_tag="VE308_00560"
     CDS             3273..3476
                     /locus_tag="VE308_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99491.1"
                     /translation="MIIKNTDPYKMKKCVSCRRDIAVNMKYFTYPLSLQQICIDCAEK
                     EIPKTIELLQKDLKKIREEKDRR"
     gene            complement(3774..>4915)
                     /gene="cysS"
                     /locus_tag="VE308_00565"
     CDS             complement(3774..>4915)
                     /gene="cysS"
                     /locus_tag="VE308_00565"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZA99492.1"
                     /translation="EEISRRYIDRYFKDFDSLNTMRADYHPKATDHIKEMIQLIDGLM
                     KKGYAYLTLNGVYFRIKSFPGYGKLSKKSVEELESGARIEIDPSKEDPLDFALWKFYS
                     DETVWPSPWGNGRPGWHIECSAMSRKYLGDTFEIHGGGHDLVFPHHENEIAQSESYTG
                     KQLAKIWVHIGMVTINSQKMSKSLGNIVTIEQALKTWRANPLRLYCLSAHYSKPLDYT
                     DELLEESIQRWRQIETCAYELRFAVGSGGEIDSVQRMCDEAMRAFEAAMDEDMNTSLA
                     VAELMKLATQLNRYIAADKLTKDMADSALPTLKKIMDILGLKIVETSDKERKEIEEIV
                     VQRNKLRAEKKFKEADEIRKKLLERSVELLDHKGRTVWVKREKPE"
BASE COUNT         1345 a         1061 c         1140 g         1369 t
ORIGIN      
        1 tttgttcatt ctgagcacat ctacttggcc actgtgtagc caatccttca gctcatcatg
       61 cgatggcttg gcgtcgtgct tgcctgccaa atgaagatgc agaagaacat agttaatttg
      121 gttgagtaag ggcttgtttg attcgtcacc tttgcgagct ttttgcttca agtcggctgg
      181 aactgtattc caccagtcat caaattttct aaccatacca acagtctaag aactgaactt
      241 attaagtgtt ttcggttttt acaaaacact gttatacagt attcgcgaca aaacttctgc
      301 aacccattag aaatataagc attgagaaaa aagagcaagc ttgtgccctt cgcaattcat
      361 gagctgcata gtgcgtatag agcctatcag cagtttcttg attgcaaccc gcctctccac
      421 catgcacgct acatcttccc ttttttcagg ggttcgtatt ttgcatatcg taaggtagat
      481 gtacttcgga ctgtagagat tgcgaaggca atttctgcta atccaatcta tgtcgatatc
      541 gggtgcggct acggcgactt tctcgataaa gtgcgacagc tgttgccgga tgcaagggga
      601 atagaaagag acggaagcat cttttatgca tttgagattt caaagcctga ttacattagc
      661 ctgatgccag ctgagaattt gtctgagcca gtcgatgtag catttgtcgg ctggatggag
      721 cagggccagg actttcgtag gtttgtagcc aaatgcgcca agtgtgttat tacaaccttt
      781 gatacaggtg gccagtgcgg cataaacagt gcctgtgaat atgaagaatt tggcttccaa
      841 agagtggcat ggtggagaac gccgtcgtgg atagatgtaa acggtgagct catgaaccgc
      901 tattacgcgc cttcacttga ctcaggcaag aaggagcagt tagcaaaatt gcgcaccgca
      961 cacaactttt ggtatgtgta tgcaaagctg gaacttatcg acaagatcaa gtcaggcttg
     1021 caagggtggt tggaaaaagt agaaaatcca aatgataggt tcgattttga gtctatcctt
     1081 gatgaatgcg gcttccatta catggaggag ctgccagcac ttgcaaacaa caggaagctg
     1141 tgggaagtga agtttgacta aatgccattt aatctggaag atctgcgcaa ggccaagcat
     1201 gagctggcgc aatttgggtc aaacaggggg tcaccaaacc atgagcgcta catctttcct
     1261 ttttttaaag gatcttactt tgcatacagg aaggtcgatg tactgcgcac ggtcgctgtt
     1321 gcaaaggcaa tctcaagtaa tccaaaatac ctcgatgtag gatgtggcta cggagatttc
     1381 cttaagcagg tacggaagtt tattccaagc gctgaaggaa ttgaaaagaa tgcggggatt
     1441 ttttatggtc ttggcattat aaaaccagat tatattagca ttgccgacgc gcattggata
     1501 gacaaaaaat acgaccttat cttcgtgggt tggatggagc caggccagga ctttcgcgat
     1561 ccaattgcgc gaagcacaga tgttattatc acgacacttg atcagggcat cagcttggaa
     1621 gcagaatttg agggtcatgg cttccaaaga gtggcatggt ggaggactcc ttcatgggag
     1681 gatgtcaata tagagatcat gaatcggtac tatacaaaaa ttccagacag caagcgaacc
     1741 gagcttgcaa agatgcgagg agcacacaac ctttggtatg tgtatgccaa gccacaacaa
     1801 gtccaaagcg ttagggcagc actcaaaagc caggttgaaa aagaatcatc tttcgattat
     1861 agatatgatt ttgaatctgt gcttgatgaa tgtggatatg gctatttgca aaagctggag
     1921 agtcctgcaa tgctagaacc aatgtggcag gtgcaatgga attagcaaga tttaaagaga
     1981 gtattgctaa gagctctata tagtaacaat aaattgaaag ttaaggtttt gggagcggcg
     2041 aaggaggtcg gccgctcggc gtttctcgtc aactgtgacc atactaacat actgatggac
     2101 tatggcgtcc tgctcaggcg tgagcccatt ttcccaatgc atgtcaagcc caaggaagtt
     2161 gatgctgtag tcattactca tgcacacctt gatcactccg gatttgttcc ttccttgtac
     2221 ctgtccggtt caacaaatat tccagcactt ggcactcttc caacttttga gctttccgaa
     2281 ttgcttattg aagacatgat taagctctct ggattctatc tgccgtttga atacatcgat
     2341 gttatgacga tgctcaaaag ctccaagaat ctgcaatata gagaacctta catggtaaat
     2401 aatgccaagg tgacccttca tgaatcaggc catgtcgtag gtggtgcaag cattgtagta
     2461 gaacacgaag gcaagaaaat attttatagt ggtgacatca atacccgtgg ctcaaagctt
     2521 ttgcgacctg cagaccttga ctttggcgaa attgacatga tgataataga gagcacttat
     2581 tctcaatcag aacagcaacc ccgcgaagag gctgaaaagg agctcattga atttgcatat
     2641 gaagtcatag ggcgcggtgg aacccttttt atcccgtctt tttcagttga gcgcgcgcag
     2701 gaaattgcaa ctgtgctcaa ggcctacaat ttcaaacaca aggtggtgat ggacggcatg
     2761 gctcttaaag caaatcagat aatgagtcgc catcccgcat ttctcaaaga tgcagaaatg
     2821 ttcagacgag ccataggtga ggccgagtgg atacagggct ggagcagaag gcgcagagta
     2881 gtagaggagc catgcgtcat aatttcgcct gcaggcatgc tggtcggagg ctctgcaata
     2941 ttttacacgc agcagatagc caacagcgag aagaatggta ttgcgatggt gtcataccaa
     3001 ggtgaaggga cacctggcag aatgctctta gaaaagcgtg ttgccaactt tgacggcaag
     3061 ataagaaaat gctacgctga tgttaagcgc tttgaatttt ccggtcacaa tagcaggagt
     3121 gagctttttg aaatcttgga tcgtgtcaaa ggcaacccaa aggtgctgac tgttcatggc
     3181 gatggtccat catgcgtcaa gtttgccgaa gagataaagg aaaagtatgg ctatgacgcg
     3241 catgcacccg gccaaggtga agtgatcgag gtttgatcat caagaatacc gacccataca
     3301 agatgaaaaa atgtgtatca tgcaggcgtg atattgcagt gaatatgaaa tatttcacat
     3361 acccgctttc cctccagcag atctgcatag attgcgcaga aaaagaaata ccaaaaacta
     3421 ttgaattatt gcaaaaagac ttgaaaaaga ttagagagga aaaagatagg cgctgatcat
     3481 agatacctcc agcctagttt tcaatttgcc ccttatttac gccatgatta tgcagacacc
     3541 tctgtgaaga ggaaactgtc aagaatcttg tccttcaact ttgaagttgc cgctgtgggg
     3601 ggatgtagat cagctatatc ccatctgcta gtgttattac tacatgctca aagattgtga
     3661 ctgaatcaat tagataacat tccatatatt catcgtcgtt gtcattactc tagtataagc
     3721 gtaagattat ctcattagtt tgaatattta catgcacaaa aactaaaatc tggctattcg
     3781 ggcttttccc ttttgaccca cactgtacgt cccttatgat ctaatagctc aacagagcgc
     3841 tctaatagct ttttccggat ttcatctgcc tctttgaatt ttttctcagc ccgtagcttg
     3901 ttgcgttgta ccactatttc ctcaatctct ttcctttctt tgtcgctagt ttctacaatt
     3961 ttcaatccca gaatgtccat tatcttttta agagttggca atgcactatc agccatatct
     4021 tttgttagct tgtctgctgc gatataccta ttgagctgtg ttgcaagctt cattaactct
     4081 gcaactgcaa gtgatgtatt catgtcctcg tccattgcgg cttcaaaagc tcgcatcgct
     4141 tcatcacaca tcctctgcac agaatcaatc tcgccgccgc tgccgaccgc aaagcgcagc
     4201 tcgtaggcgc atgtctctat ctggcgccac ctttggatcg actcttccag taattcgtca
     4261 gtataatcaa gcggtttcga atagtgtgct gacaggcagt agagccgtag gggatttgct
     4321 ctccatgttt tcagcgcctg ctctatcgta acaatgttgc caagcgattt gctcatcttt
     4381 tgtgaattga tggtaaccat gccgatgtga acccatatct tagcaagctg cttgcctgtg
     4441 tatgattctg actgggctat ctcattttca tggtgtggaa aaactagatc atgtccgccg
     4501 ccatgaatct cgaacgtgtc gccgagatac ttgcgcgaca tggcagaaca ctctatatgc
     4561 cagccgggcc tcccgttacc ccaagggctt ggccacaccg tctcatctga atagaacttc
     4621 cagagcgcaa agtcaagtgg gtcttctttt gagggatcga tttctatcct cgcgccggac
     4681 tctagctcct caactgactt ttttgataac ttgccgtagc ccggaaatga cttgattctg
     4741 aaatatacac cgttgagcgt caggtaggca tacccctttt tcatcaggcc atcaattagc
     4801 tggatcattt ccttgatgtg atcagtagcc tttgggtggt aatctgcccg cattgtattc
     4861 aagctgtcaa agtctttgaa gtatctgtca atgtatctcc tcgatatttc ctctg
//
LOCUS       DASQTF010000025         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_97133,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000025 DASQTF010000000
VERSION     DASQTF010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97133"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..961
                     /locus_tag="VE308_00570"
     CDS             125..961
                     /locus_tag="VE308_00570"
                     /inference="COORDINATES: protein motif:HMM:NF037920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdvA-like protein"
                     /protein_id="HZA99493.1"
                     /translation="MSEEKLGFVGKQVKDIYGTYIGRAVGIITENDGEIEAVGVDCGS
                     GGLKQLPYEQLVIQGEYVFFIPRWRLDAQRLLRQKSLTLKRIKALQDILSENDSMKDD
                     AELVYIKYEKNLHELDETGKQVNDKLNARLAELDLEVKNIKAVLFDAKLQFRSNEMKE
                     ETYQQVKVQTDELIEHINNERAEISNVKEKLTTQTLENIATTDSSSSSSTLAAPPTKE
                     EEQETRTEQAPSQPAEVVAVAQVSAASSEEKEEDVNDAPASSSNSVEHSETNWLNQVI
                     TK"
     gene            complement(1063..1323)
                     /locus_tag="VE308_00575"
     CDS             complement(1063..1323)
                     /locus_tag="VE308_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZA99494.1"
                     /translation="MKYRSRTEIVAMILEAANGGATKTKIMYKAFLSYAQLREYLAVL
                     IENGLLEYMEGTQTYKTTAKGLNFLKMHSEIGELLQTSVRER"
     gene            1465..3429
                     /gene="acs"
                     /locus_tag="VE308_00580"
     CDS             1465..3429
                     /gene="acs"
                     /locus_tag="VE308_00580"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZA99495.1"
                     /translation="MSSPSRLDENSGNVSSDKMKVSIRIGKRLEKISKEALAEPDKFW
                     AAQARNLIWFKEWDHILDWKPPFARWFVGGHLNASVNCLDRHIDTDTKNKAAIIWESE
                     SGETRTLSYFQLYGQVNKFANVLKSLGVNKGDRVTIYMPMVPELPIAMLACARIGAIH
                     SVVFSGFSAQALIDRANDAKSKVIVTADGGIRKGRLVELKKVVDESLLSLPSVEHVVV
                     LKRAGNKVNFGPKDLWWHELMEDAKLYCQPEPVESTHPLYILYTSGTTGKPKGVMHGT
                     GGYLTHLYATAKWVFDFTKQDIFFCTADIGWVTGHSYIVYAPLMHGLTEIMYEGAPDF
                     PKPNRYWAIVEKHGATILYTTPTALRMYMKYDNDGVVSNSFDLSSLRLLGTVGEPINP
                     KVWIWYFKRIGNENCPIVDTWWQTETGGIMLSMCAGIETIPMKPGSATFPVPGVDAIV
                     VDETGKQVRPGTKGYIVICKPWPGMFLSLWGDDEKYRQTYWTKFPGLYYPGDYALVDE
                     EGYLWLLGRADDVLKVAGHRLGTMELENAFVSHKAIAEAAVTAKPDDTKGESIIAFLV
                     LKEGFSDSEDLRKEMVAHIRSTLGPIAAPDEVYFVSKLPKTRSGKIMRRLLKSIASGD
                     KIGDITTLEDEAAIDEVRQAYEDLKKMVNS"
BASE COUNT         1066 a          736 c          843 g          906 t
ORIGIN      
        1 attctactaa aagcaatcaa aatcttataa gaatcatcta aactaaatgg catgattaat
       61 tccactattt tatcgaactg ttttaacacc gttatcatta tacactcttt gctcaactat
      121 tcacatgagc gaagaaaagc ttggatttgt aggcaaacaa gttaaggaca tatatggtac
      181 ttacatcgga agagccgtcg gcataataac agaaaacgat ggcgagatag aagccgtcgg
      241 agtcgattgt ggttctggag gtctaaagca actgccttac gagcagctgg ttatccaagg
      301 agaatatgtc ttctttatac ctagatggag gcttgacgca cagaggttgc tgaggcaaaa
      361 gtccctcacg ctcaagagga taaaggcact tcaggatatt ctctctgaga atgactctat
      421 gaaggacgat gcagagctag tgtatataaa atatgagaag aatctccatg aacttgatga
      481 aactggtaag caggtaaatg acaagctcaa cgcaagactg gcagaacttg acttagaagt
      541 taagaacatc aaggcagtcc tgtttgatgc aaaactccag ttcagaagta acgaaatgaa
      601 ggaagagaca taccagcaag tcaaggttca gacggacgag ctaatagagc acataaacaa
      661 cgaaagggct gaaataagca atgtaaaaga aaagttgaca actcagacgc tggaaaatat
      721 cgctacaact gatagtagta gtagctcttc gacacttgct gctccaccaa ccaaggaaga
      781 agaacaagaa acgcgaactg aacaggcacc aagccagccc gcagaagtgg ttgctgttgc
      841 acaagtgagc gcagcaagct cagaggaaaa agaagaagat gttaatgatg cccctgcgtc
      901 ttcttctaac agcgtggagc actcggagac aaactggctc aaccaagtga taacaaaata
      961 attgtgtagg gccgagcaca catatacaca cacggacact atgtccggtc agttctcttc
     1021 attttttaaa aatcattttt taaaaaattt agatgggcag ctttagcgtt ctctgacgct
     1081 tgtctgtaaa agctccccaa tctcactatg catcttcagg aagttaagcc cttttgcagt
     1141 tgtcttgtat gtttgtgttc cttccatgta ttcgagcaga ccgttttcga ttaagacagc
     1201 gaggtattct cttagttgtg cgtagctgag gaaagcttta tacatgattt ttgtcttagt
     1261 tgctccgcca ttcgctgcct ctaggatcat tgctactatt tctgttctac ttctatactt
     1321 caaactatta tagaagtgat ttttctggat ttttaatata tcgattgatt cttgaataac
     1381 aaaattctaa acggcttcta catttaatct tgaaaagata gttaaacaaa tactttaaag
     1441 atgagtttta gttcttcaca atgtttgtcg agtccttcaa gactagatga aaatagtggt
     1501 aatgttagta gtgacaaaat gaaggtaagc atcaggatag gcaagcgcct tgagaaaatc
     1561 tcaaaagaag cgctagcaga gccggacaaa ttttgggcag cgcaggccag aaatttgata
     1621 tggttcaaag aatgggatca catccttgat tggaagcctc catttgcaag gtggtttgtc
     1681 gggggtcatc tcaatgcatc tgtaaactgt cttgacaggc atatcgacac tgacacgaaa
     1741 aacaaggccg caataatctg ggaaagcgag agtggagaaa caaggacgct ttcctatttt
     1801 caattatatg gtcaggtaaa caagtttgcc aatgtcctga agagccttgg cgtcaacaaa
     1861 ggcgaccgcg tcacaatata catgcctatg gtgccagagc ttcctattgc aatgcttgca
     1921 tgcgcaagga ttggtgctat tcactcagta gtattttctg gcttttctgc acaggcgctt
     1981 attgacagag caaatgatgc caagtctaaa gtcatagtaa ctgcagatgg tggcataaga
     2041 aaaggccggc ttgtagaact aaaaaaagtg gtggatgagt cacttctatc cctgccctca
     2101 gtggaacatg tggtagtgct caagcgcgcc ggtaataagg tcaactttgg accaaaggat
     2161 ctctggtggc atgagttgat ggaagatgcc aaactatatt gccagccgga gccagttgaa
     2221 agcactcatc cactttatat tctctatact tctgggacga ctggcaagcc caagggcgtg
     2281 atgcatggaa ctggtggcta ccttacacat ctctatgcta ctgccaagtg ggtctttgac
     2341 tttacaaaac aagacatctt tttctgtact gcagatatcg gctgggtcac gggtcacagc
     2401 tatattgtct atgctccact gatgcacggg ttaaccgaaa taatgtatga aggagcgcct
     2461 gattttccca agcctaacag gtattgggcg attgtggaaa agcatggtgc cactatactg
     2521 tataccacac caactgcact ccgcatgtac atgaagtacg acaatgacgg cgtagtgtca
     2581 aactcgtttg atctttcgtc actccggctg cttgggacgg tgggtgagcc tattaacccc
     2641 aaagtatgga tatggtactt caagaggatt ggcaatgaga attgcccaat agttgacact
     2701 tggtggcaaa cagagactgg agggataatg cttagcatgt gtgctggaat cgaaacgatc
     2761 ccaatgaaac ccggctcagc cacgtttcct gtacctggag ttgatgcaat agtggttgat
     2821 gaaaccggca agcaagtaag accgggaaca aaaggctata ttgttatttg taagccgtgg
     2881 ccagggatgt tcttgagtct ctggggtgac gatgaaaaat accggcagac ttactggacc
     2941 aagttccccg gcctctacta tcccggtgac tatgcactag ttgatgagga gggctatctg
     3001 tggctccttg gcagggcaga tgatgtactt aaggtggccg gccacaggct tggcacaatg
     3061 gagttggaga atgcatttgt ctcacacaag gccattgcag aagccgcagt gacggccaag
     3121 cctgacgaca caaaagggga gtcgataatc gcattcttag tgctaaagga aggcttttct
     3181 gactctgaag acctgcgtaa agaaatggtt gcccacattc gaagcacact tggccccata
     3241 gcagcgccgg atgaggtata tttcgttagc aagctgccca agacgcgtag cgggaagata
     3301 atgaggaggc tgctaaaatc cattgcaagt ggcgacaaaa tcggcgacat caccacgctt
     3361 gaagacgagg cggctattga cgaagtcaga caggcctatg aagacctcaa aaagatggtc
     3421 aattcttaga acttccaagt ggccagatat gatggagtta tctcaataac gcaagaatct
     3481 ggacccatgg caagtatggc ctttgcagtt ttactctgca gcttgcccaa gtacctcttt
     3541 acattacgtc t
//
LOCUS       DASQTF010000026         4236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_282585,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000026 DASQTF010000000
VERSION     DASQTF010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282585"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VE308_00585"
     CDS             complement(<1..604)
                     /locus_tag="VE308_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99496.1"
                     /translation="MVVHPFKRATKVLKFYRDYSASIPDDMNTICALLTSPEGHPIVA
                     IVVCYHGDIMDKGQQILQPLREFGPPLADLIRPMSYTEAQSMLDLAIPPSALRSYWKS
                     HFLKEIPDQAIDTLISYFAKVPSPRTAILFQQFGGEASRVREGTTAFGHRKDGEYDFI
                     IFSLWQISSDDDASIRWTRGLWDEMKPFSPGGVYVNNLGDD"
     gene            complement(674..1141)
                     /locus_tag="VE308_00590"
                     /pseudo
     CDS             complement(674..1141)
                     /locus_tag="VE308_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(1556..1732)
                     /locus_tag="VE308_00595"
     CDS             complement(1556..1732)
                     /locus_tag="VE308_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99497.1"
                     /translation="MRLIPKNYRFTVKLVSLYDRRNFSGYGTAGRIESETFGMTRKSK
                     KKKKMTRTDGKARS"
     gene            complement(2048..3028)
                     /locus_tag="VE308_00600"
     CDS             complement(2048..3028)
                     /locus_tag="VE308_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZA99498.1"
                     /translation="MVDIILGRWKSQILYAGVKLGIFDAIDTMPKHPSEIAQELGLDL
                     LLSYRLLRALASIGFLNEKDDRSFYITPLGELVRKDHPETLRGIILLEEGPEHYAVWK
                     HLISIVKDGKQDGFIREFGLKIFDYLSQNPGYAEVFNQAMSSYSAIHTRQVLEALKNY
                     DFSKIQSLCDIGGGHGHLICNILREHPRLKGMVLELESTIKNKELLWAEKMGVVDRCN
                     YLAGNMFLEVPSSDAYIMKMILHDWNDEECVKILSNIQKASPAGGRVLVVEHVVPGPE
                     FPHFSKLFDIHMMCALTGRERTEKEYAELLEKAGLQYMCKHITRHQNQLG"
     gene            3232..4092
                     /locus_tag="VE308_00605"
     CDS             3232..4092
                     /locus_tag="VE308_00605"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZA99499.1"
                     /translation="MSTITLRPGTLDDAKRCGEICYSAFRVIAEEHNFPPDFLSPELA
                     ITLLSEFMAHPDIYGVVAELDGQVVGSNFVDERSIIAGIGPITVDPTVQNRAIGRELM
                     QHILARVAKRRYPGVRLVQAAYHNRSLSLYSKLGFVVREPLSIMQGQPLALQIPGYIV
                     RTATVGDLDACNQVCLRVHGHNRGGALLDAVRRGTATVIEHNSRITGYATAVAFFGHA
                     VGETNEELKALIGAAPAFLGPGFLLPSRNAELLRWCLKHGLRIVQPMTLMSIGLYNEP
                     AGAFLPSILY"
BASE COUNT         1132 a          997 c          865 g         1242 t
ORIGIN      
        1 cgtcgtctcc caagttattg acataaaccc ctcctggtga gaagggtttc atttcgtccc
       61 atagtcctcg ggtccagcgg atactagcat catcatctga agagatttgc caaagtgaaa
      121 atattatgaa atcatattct ccatcttttc gatgcccaaa agcggttgtg ccttctctaa
      181 ctcgcgaggc ttcaccaccg aactgctgaa acaatattgc ggtccgcgga gaaggaactt
      241 ttgcaaaata actaatcaaa gtatcgatag cctgatcagg aatttccttc agaaaatgtg
      301 atttccagta gctacgtaat gctgatggcg gtattgctaa atcaagcatt gattgagcct
      361 cagtgtaaga cattggtcgg attagatccg ctaatggagg accaaattcc cttaatggtt
      421 gtaatatttg ctgtccttta tccattatgt cgccatgata gcaaaccaca atcgctacta
      481 taggatgacc ctctggagaa gtcaatagag cacaaatggt attcatatcg tcaggaatag
      541 atgcgctata atcacgatag aacttaagca cctttgtagc tctcttaaaa ggatgcacca
      601 ccataccagc tagcacttgt gggcctacag cataaagttg gtattcaaag gatgttgcta
      661 taccaaagtt acctcaacca cctctaatac cccagaagag atccttattc tctgcatcgc
      721 tggctttgac gaactgacca tcggcagtaa caatatcgac agagagcagg ttatcacagg
      781 tcaagccata tttgcgcgca agccagccaa ctcctccgcc tagcgtgagt cctgaaatcc
      841 cagtatttga aatgacacca agtgtggttg ctaatccgaa tgcttatgtc tctctatcaa
      901 attcgcctag cctcaggcct ggctgagcat gagctacttt gcgagaaggg tcaaccccga
      961 cccctttcat caacgacaag tcaatcataa tcccatcatc acaaactgct tttccgccta
     1021 cattatgacc accagctcgg acagagacca acaaattatt tgcgagtgca aagcttatac
     1081 ttttgatcat atcggctact ccgatacagc gtacaattaa tgcaggtcgc ttgtcaatca
     1141 ttgcattcca tacttttcac acttcattgt agctaagatg ctccggaagg atgagttgcc
     1201 cgctaagtaa agtcctaaat tcttgaataa catcatcact gatgcggcta gccacgactc
     1261 tttttcccac cacatatgcc atacgcctgt tttttacttt ttattccaaa cctcagtcta
     1321 gctttaacct ttagtattta tcatggtcta tatcatcatc cttgcgatga taatggctca
     1381 aaaatcaaac ttgttctcta tacccatgct ttcatggagc cgccagaaat tcaacccgtt
     1441 gacatgagca gattttcttt tttgtttgcc tctcgataca agctcgtggg cgctatatga
     1501 atagcaattt tgctgtttat tcaagatccc gcatcttaag ggtggcgcct cttttctaac
     1561 ttctcgcttt accgtctgtt cttgtcattt tcttcttctt ctttgattta cgtgtcatgc
     1621 caaaggtctc tgattctatt cttccagcgg taccgtagcc tgaaaaattt cttctatcgt
     1681 agagagacac taattttact gtgaagcgat agtttttagg tataagccgc actatattag
     1741 gttgggaatg aggtgagttc gctgatccga ctgagcaaaa gcgatgaaga tctcacgacg
     1801 atccgaccca aatttctaca agatttcata gatttggcat tgagctgcgt ttcttaatag
     1861 caattcatga gattgtttat aatccggatt ctatacaaac ttttctgtgt cttactctct
     1921 ttgccttctc taaagatttt caagcgggtg cggaagttta tgcttctcca gagtaccttg
     1981 atctgaattc gtcatcattg tatgaacaaa aaattgcaat tagatagcta ttgcttgatg
     2041 ccttcgacta ccccaattga ttttgatggc gggtaatgtg tttgcacata tattgtaacc
     2101 ccgctttttc tagcagttct gcatattctt tttctgttct ttctcttcca gtgagagcac
     2161 acatcatatg tatgtcgaat agttttgaga aatgagggaa ttccggaccc ggaactacat
     2221 gctcaacgac aagaactctt ccgcccgccg gagatgcttt ttgaatgttt gacaaaatct
     2281 tgacacactc ttcgtcattc cagtcgtgca gtatcatttt cataatatat gcatcagagc
     2341 ttggaacctc aaggaacata ttacctgcga gatagttgca gcggtctacc actcccatct
     2401 tttctgccca caatagctcc ttatttttga tagtagattc caattcaaga accatgcctt
     2461 tgagccgggg atgttctcga agtatgttgc aaatgagatg cccatgacca ccgccaatat
     2521 cacatagact ttggattttt gaaaagtcgt agttcttgag agcttcaagc acctgtctcg
     2581 tatgtatggc agagtaactg ctcattgctt ggttgaacac ttcagcataa ccaggattct
     2641 gtgagagata atcaaatatt ttgagaccaa actcacgaat gaaaccgtct tgtttgccat
     2701 ctttaactat acttatcaaa tgcttccaaa cggcatagtg ctctggacct tcctccaaca
     2761 aaattattcc ccttaatgtt tctgggtggt ctttgcgaac taattctcct agaggcgtaa
     2821 tgtaaaagct ccgatcatct ttttcattca aaaatccaat agaggcaaga gcccttaata
     2881 gccgataaga taataacagg tcgaggccca attcttgagc aatctcagaa gggtgctttg
     2941 gcatggtatc tatagcatca aagattccta attttacacc ggcgtataat atctgacttt
     3001 tccatctccc gagtattata tcaaccactc tttcatgatt agacatatga gttgaacacg
     3061 tggttatagg tattaagagg tcggccgtag gtcttaactt ttccattaat tctttgttaa
     3121 catatagaaa aaactgttgc ggaatgatta aggcgttaat tatagttagg cgcccgggtc
     3181 caactgataa ttaaaaataa tggagaacag caaatgaagg aagaaatctt gatgagcacc
     3241 atcacattac gaccaggtac acttgacgac gccaagcggt gcggcgaaat ttgctatagc
     3301 gcatttagag tcattgcgga ggagcacaac tttccacccg actttctctc ccccgaactc
     3361 gccatcacac tgttatctga atttatggct caccctgaca tttacggagt tgtcgccgaa
     3421 ctcgatggcc aagttgttgg gagcaacttc gtcgatgaac gttcgatcat tgccgggatc
     3481 ggcccgatta cagtcgatcc aaccgtgcaa aaccgcgcga ttggacgcga gttaatgcag
     3541 cacatcttgg caagggttgc aaagcggcgc tatccggggg tccgattggt ccaagcagcc
     3601 tatcataacc gctcgctttc tctctactca aagctagggt tcgtggttcg cgagccgcta
     3661 tccatcatgc aagggcagcc gcttgcatta caaatccccg gatacatcgt tcgtaccgca
     3721 acggtaggcg atctggacgc ttgcaatcag gtttgtttga gagtacatgg ccataaccga
     3781 ggaggcgcgc tacttgatgc ggtcaggcgt ggaacagcca cagtaataga gcataacagt
     3841 cgtatcacgg gctatgctac agcagtagcc ttctttggtc atgctgtggg cgagacgaat
     3901 gaagagttaa aagcacttat cggcgcggca ccagcgtttt tgggtcctgg tttcctgctg
     3961 cccagccgca atgcggagct gttgcgttgg tgtttaaagc atggactacg catagtccaa
     4021 ccgatgacac tcatgagtat cggtctctac aatgagcccg cgggagcatt tttaccatcg
     4081 atactttact gattgctatc caaacttgac attcactttt tccgcgttag cgtcaacatg
     4141 gcttacaagt tgaactagtc tcatttttgt cgcattttac agagattttc taacaggctt
     4201 tgggggatcc ggcagaaaac cttcacaaca ggcagt
//
LOCUS       DASQTF010000027         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_97297,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000027 DASQTF010000000
VERSION     DASQTF010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97297"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VE308_00610"
     CDS             complement(<1..825)
                     /locus_tag="VE308_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZA99500.1"
                     /translation="MEIVYDYPLYRPPSEAESTIFQVTLGCSFNKCSFCNMYRSKEYV
                     ERPWEQIKSEIDMAAKFYPDTRRIFLADGDALNLPKLRMIEILKYLRANFPRLERISC
                     YAMPKNILQKKDDELREIRAAGLHMMYVGIESGNDIVLKKVTKGATYKTILQACQKIK
                     HHGYILSCMVIVGLGGKTYTKDHITDTARILSETSPDYVGALTLHLEEAIYEEFMNKF
                     GEPFVPLDDLDILDELERLVADFKPTGQVIFRANHASNVYSIGGTIPQDTEKLLALI"
     gene            1801..>2463
                     /locus_tag="VE308_00615"
     CDS             1801..>2463
                     /locus_tag="VE308_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99501.1"
                     /translation="MTETTDQTETSPDGIVTVAIGEGSNETIQHYTYDPRTVEINAGE
                     SVTWFTPAEFPDLHTVTFVQDPSIVSDILLPFAVPAGGGATDFELLPPFNTGEPVIIP
                     TPDGREAIVALNKHGWYPAVVGANNQTTYLEGTDIQATLNSTVRALNSGIILPPMPPT
                     GEAQPNSTETGTAGEQQLGPPFPIVSSFTVTFEEPGTYPYFCAIHPWMTGQVVVSGDI
                     PAE"
BASE COUNT          694 a          555 c          505 g          709 t
ORIGIN      
        1 aatcaaagca agcagctttt cagtgtcttg aggtatcgtg ccgcctatag aatagacatt
       61 tgaagcatgg tttgctcgga agatcacctg cccagtgggc ttgaagtcag cgacgagcct
      121 ttctagctca tccagaatgt ccaaatcatc gagtggcaca aatggctcac caaatttatt
      181 catgaattcc tcgtatatgg cctcttcgag gtgaagagtt agcgccccaa catagtcagg
      241 agacgtttcg ctcagtatgc gagcggtgtc tgttatatgg tctttggtat aggtcttgcc
      301 tcctagtccc acgattacca tgcaggacag tatgtagcca tggtgcttga tcttttggca
      361 cgcctgaaga attgttttgt aagttgcgcc tttggttacc ttctttagca caatgtcatt
      421 tccgctttcg attccaacat acatcatgtg gagtccagca gcccttattt ctctgagctc
      481 atcgtccttc ttctgcaaaa tgttctttgg catagcataa catgaaatcc tttcaagtcg
      541 ggggaaattt gccctgaggt actttagaat ctctatcatc ctgagctttg gaaggttcag
      601 tgcgtcaccg tctgctagaa agatcctgcg tgtatccggg tagaacttgg cggccatatc
      661 aatctcagac tttatttgtt cccaaggtcg ctcaacatat tctttggaac ggtacatatt
      721 gcaaaatgaa cacttgttga aggagcaacc aagtgtcact tgaaatatcg tagactcggc
      781 ctctgatggt gggcggtaaa gagggtaatc ataaactatc tccattctta cagcaacgat
      841 atatagagat gatatgcatt aataattgtt ttttatgcag gactcacttt tctgagcaac
      901 agctaactat gtctttgcat atgaagtata ttttgcatca tagacgtcat ttttaaggtc
      961 ttagaggaac aagacgaaaa cgatcacact aaagcaaatc ctgcaggatt tgtaacaatg
     1021 ggtttaaact gggacaaatc aatgcatctt gcattaacct tgcagaactg atgaaataca
     1081 aattagggtc agttcgtcgc gagatcttca tcgctttttg ctcagcctgg agcagcaaac
     1141 tcacttcatc cccctttttc ctaagacgag gaagtcactt aaatcttagc actaaacggt
     1201 atattctgga gcagcaccat gatatgatat atcaattgcc gatacctaca gtatcaacac
     1261 ttttaggatg caaattcatg cggcgggagt gacagctgtc aaaacaactc tctgctgcgg
     1321 tacccttcgt ctcacctact ttttcgcttt atgaatattt ctacaattct ctatattcta
     1381 ctttacttgc gatgaaaggt acacatcata tggatatttc tagatcctgt cagatgattg
     1441 aatagattct tccgcaagaa atgttgtccg cgttaattga gaaaactaag tgtcggacaa
     1501 attctgtttg ctaattatta tctcttattc tacattttat catctacgat tcaaatgcaa
     1561 ggcgccatta ttcatcagca ggcaaatgca cttgctcttc attctttctt ggttgaatag
     1621 cctaatagac agccattatg gattaaagaa gatgagaaat ctatcgaaga attccttaaa
     1681 tgcgaaatat cattatacat tttgatgaaa tcaaattctt actatgttgg gatcctagcc
     1741 gcggcccttt tagtagttag tgggccagct agtttcgcaa ccccaattga tcaaacccaa
     1801 ttgactgaga caacggacca aacagaaaca tcgccagatg gaattgtaac agtagcaatt
     1861 ggtgaaggca gcaacgagac tattcaacac tatacatacg atccacggac agttgaaata
     1921 aacgcaggag aaagtgtcac ttggttcacc ccagctgaat ttccagatct ccatactgta
     1981 acctttgtcc aagacccgag cattgtatct gacatactat tgccatttgc tgtccctgct
     2041 ggtggcggcg caacagactt tgaactgcta cctccattta acaccggtga gcctgtcatt
     2101 ataccgaccc ctgatggcag agaagcaatt gtagcgttga acaaacatgg atggtatcct
     2161 gcagtagttg gtgcaaacaa tcagacaacc tatcttgaag gaacagatat tcaagccacg
     2221 cttaacagca ctgtacgggc actaaattct ggtattatac ttccgccaat gccacctacg
     2281 ggagaagctc aaccaaacag caccgaaaca ggcactgcag gagaacaaca attgggtccg
     2341 ccatttccga tagttagctc ctttaccgtg acatttgaag agcctggcac gtatccatac
     2401 ttctgtgcaa tccatccctg gatgaccggt caagtggtcg tgagtgggga cattccggct
     2461 gaa
//
LOCUS       DASQTF010000028         5761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1022713,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000028 DASQTF010000000
VERSION     DASQTF010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022713"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1220)
                     /locus_tag="VE308_00620"
     CDS             complement(135..1220)
                     /locus_tag="VE308_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HZA99502.1"
                     /translation="MLCIDRDNDIGSKGGIDTPIVGRDACINAGTRLALEDPEDADSN
                     AIFGAVKIYEELVSKGYEAEVAVVAGKFNKGVEADEKISLEVKSILEKYRADGAVIVS
                     DGEDDETILPVIQSRIPVISVRRVIIRHSRSVEYSYAVLGRYLRMLVYDSQYSKFFLG
                     VPGILLAAGALAVIFDAAKWITAIVLAILGGAFIVRAFDIDKALSSFGNMLTPTSFIK
                     IFSIIGGVLIILAAIANGISSIPSNILTPEMSALDIVSNRTIVGRFADSTITLLWIGI
                     ATILGGVLLSDWFKGSSRIMSDILRLAVLALLYIPLLQFTSVLTEGTSPFTLISSLLI
                     GLAVSLVAATVLYYYFRNRKGGEVLKH"
     gene            complement(1436..2029)
                     /locus_tag="VE308_00625"
     CDS             complement(1436..2029)
                     /locus_tag="VE308_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HZA99503.1"
                     /translation="MRLADARRDQYRRLAKDQGYRARSAYKLLQINRSYNIIKKGDKV
                     VDLGCAPGGWLQVAMKEVRSSGKVIGVDLKQVTPVAGATILQGSIEDPNVLSKIAEIL
                     GCKADVVLSDMAPNVSGVWDIDHARQISLSTIALGFVQQVLRVGGSSVFKVFEGDMLK
                     EFKSELRKSFGKVLLNKPSASRQESSELYIICLDFKQ"
     gene            complement(2026..2424)
                     /locus_tag="VE308_00630"
     CDS             complement(2026..2424)
                     /locus_tag="VE308_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZA99504.1"
                     /translation="MKLNEDQIEDLTGKVFRTIISNGKDGILQSELWKELDLTSRDGS
                     RVAIRLEKRSLIRREKMLENGRWTYRLFAVKLPVDTTSIEQAPCLTCPFEHMCSLEGS
                     YSPTSCNLIEEWLIKSFDTANLNPAKLYKK"
     gene            complement(2572..4089)
                     /locus_tag="VE308_00635"
     CDS             complement(2572..4089)
                     /locus_tag="VE308_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HZA99505.1"
                     /translation="MTIKNGAEYIESLRGRNLKIYLFGEVVKEPVDHPMIRPSINAVA
                     ETYDLAVEEPELAAPQSSLTGMQVNRFLHITESAQDLVNQNKMQRKLGQITGTCFQRC
                     VGMDAINSLFSTTYEIDQKHGTEYHNRLVEYVKMLQRGNLMIAGAMTDVKGDRGLSPS
                     QQEDPDMFVHVTKRDSKGIYISGAKAHQTGIINSHWVVVMPTMRLKSEDKDYAIIGAI
                     PVDAPGITYIYGRQSSDTRSMEGGNIDAGNAMYSGQEAMIIFKDVFIPNHLIFMDGEY
                     EFASMLVERFTCYHRRSYVCKTGLGDVLIGAATAIADYNGVANASHIKDKLVEMTHLN
                     ETIFAAGIASSYQSHKTSAGNWQNDDMLANVCKHNVTRFPYEIGRLAQDIAGGLMVTM
                     PSEKEFRNPDTGPLLEKYLKGRKGVSTENRMRILRLIENMTLGRNAVGYLTESMHGAG
                     SPQAQRIQIARQMQLETKKKYAKRLAQIKEGEAEEMVPESTEYFDRIFGTKNKVK"
     gene            complement(4593..5078)
                     /locus_tag="VE308_00640"
     CDS             complement(4593..5078)
                     /locus_tag="VE308_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99506.1"
                     /translation="MQFIEKIASLKDFDEAFELVKAVVLQKFKLHRAGLSLILQMMPS
                     NLGAYHMLGSNAIVVNTYLLTTLKRIVKSTEEYNSYIFMVLAHEYLHSLGIVDENTVR
                     QMTFELCKWMLGNDHTATKMAKNGPSAIYPELRSLMQSQFGRDFQVVRNFDRTNQTYI
                     Q"
     gene            5183..5731
                     /locus_tag="VE308_00645"
     CDS             5183..5731
                     /locus_tag="VE308_00645"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZA99507.1"
                     /translation="MGAKAKQVNLREIIANYPDFPKQGILFRDINPVFKRNDALNYIA
                     NEFYRTYSKAKVDMVAGIESRGFIIATALALRFGRGIVMIRKAGKLPGRTVKKSYDIE
                     YGTAIMELQQDAIDKGNGILIADDLIATGGTAIAAAQMIKELGGKVAGFAFIIELSDL
                     KGADRLRKMGYNVESLVTFDGK"
BASE COUNT         1483 a         1345 c         1215 g         1718 t
ORIGIN      
        1 gtaggcgctg catagttgtg tacgtctaac cattcttctt gcgacatcca gtagccgcaa
       61 aaccactttt gttgttttgg atcatagtct ccgtgctgtt gtggcatgta atttattgtt
      121 gcagtcactt cacattagtg tttcagaacc tcgccgcctt ttctattcct aaaataatag
      181 taaaggactg ttgcagctac aagcgacact gcaagaccta tcagcagaga tgagatgagc
      241 gtaaatgggc ttgtcccttc tgtaagaacg gatgtaaact gtagtagtgg gatatagagc
      301 agcgcaagca cagcaagccg cagtatatca gacataatcc tgctgcttcc tttgaaccaa
      361 tcgctcaaca agacgccgcc aagaattgta gcaatcccta tccacagcag agttatcgta
      421 ctgtcggcaa accttcccac tatcgtccta tttgaaacga tgtcaagcgc actcatttca
      481 ggagtgagta tgttggacgg tatgctggat atgccgtttg caatggccgc aagtatgata
      541 agcacgccgc caatgattga gaatatctta ataaacgatg ttggagtcag catattgcca
      601 aatgagctta atgccttgtc gatgtcaaat gccctgacga tgaatgcacc accaagaatt
      661 gcaagcacga ttgcagttat ccactttgcc gcgtcgaata tgacggcaag tgcccctgct
      721 gctagcagga tgccggggac acctagaaag aactttgaat actgagaatc ataaacgagc
      781 attctaagat accggcccag caccgcgtat gaatactcaa cacttctact atgccggata
      841 ataacacgcc gcactgatat gacgggtatt cttgattgaa tgacaggtag gattgtctcg
      901 tcgtcctcgc catcagagac aatgactgca ccgtcagcgc ggtatttctc aagtatgctt
      961 tttacttcaa ggcttatctt ctcgtcagcc tcgacgccct tattgaactt gccggctact
     1021 accgcaacct cagcctcgta gcccttgctc acaagctcct cgtatatctt gaccgcgcca
     1081 aaaatggcat tcgagtctgc atcctctgga tcctcaagtg caagcctagt gccagcattg
     1141 atgcatgcat cacggccgac tattggtgta tcaatgccac cctttgagcc aatgtcgttg
     1201 tctctgtcta tgcagagcac taagagcttg ctataagttg cttctttgct tgcacttttt
     1261 atgctggcgt ggttaataga atacggcatc ttgctcccga tactctattg aatagtttat
     1321 agattaatta atttaatccg ctttctaata agcggaatta ttggttttat taaaaagcga
     1381 ggcctgatgg tctctgcctt atagaaattt tatatataat tctggtcatt tggattcatt
     1441 gcttaaagtc taggcagatg atatataatt cactactctc ttgccggctt gcagaaggct
     1501 tgtttaaaag cactttgcca aagctctttc gtaattccga tttgaactcc tttagcatgt
     1561 cgccctcaaa caccttgaac acgctgcttc cgcctactct tagcacctgc tgcacaaagc
     1621 ctaacgcaat tgtggaaagg gagatctgcc ttgcatgatc tatgtcccat acgccgctca
     1681 catttggagc catatctgag aggacaacat ccgccttgca gcctaaaatt tcagctattt
     1741 tgcttagcac gtttgggtcc tctatactgc cctgtagaat tgtcgcacca gcaactggtg
     1801 taacctgctt gaggtctacc ccgatgacct ttcctgatga cctcacttcc ttcatggcta
     1861 cctgcagcca ccctcctggt gcacacccaa ggtcaaccac tttgtcaccc ttttttataa
     1921 tgttgtaaga gcggtttatc tgcagcaact tgtaggccga ccttgcccgg tatccctgat
     1981 cctttgccag ccggcggtat tgatcacgtc gagcgtcagc aagtctcatt ttttataaag
     2041 ttttgcaggg tttaggtttg cagtgtcaaa agatttgata agccattctt caattaaatt
     2101 acagcttgtc ggactgtaag aaccttcaag ggaacacata tgctcaaaag ggcatgtcaa
     2161 acagggggcc tgttctatgc ttgtagtgtc aactggcagc tttactgcaa agagcctata
     2221 cgtccatcgg ccattctcta gcattttttc gcggcggatc agtgatcgtt tttcaagccg
     2281 gatcgcaaca cgagagccat cgcggcttgt caagtcaagc tccttccaaa gctctgactg
     2341 tagtatgcca tctttgccat tgctaattat agtgcgaaaa actttgccag tgagatcctc
     2401 gatctgatct tcgtttaatt tcaatcaaca caaccccgga ttggtaccat gataactatg
     2461 ttgaacggat aaataatcct ttttgagatc aagggattaa aaggcaaaat aaaagcaatg
     2521 gctgaaagat gttatagatt agcccattgc tgtctcgata gttataagag attatttgac
     2581 tttatttttt gtaccaaaga ttctatcaaa gtattctgtc gattctggca ccatttcttc
     2641 tgcctcgcct tccttgattt gtgcaagcct ctttgcatac tttttcttgg tttcaagctg
     2701 catttggcgc gcaatctgaa ttctctgtgc ctgcggtgat cctgcaccat gcattgactc
     2761 tgtcagatag cctactgcat ttcgaccaag ggtcatattt tcaatcagcc tcagtatcct
     2821 catcctattc tctgttgata cgcctttcct gccctttagg tatttttcaa gtagcggtcc
     2881 agtatcagga ttgcggaact ctttctcaga tggcatggtc accataagtc cgcctgcaat
     2941 gtcttgggca agcctaccta tttcatatgg aaaccgtgtg acgttatgct tacagacatt
     3001 tgccagcatg tcgtcattct gccagttgcc tgcggatgtt ttgtgcgatt ggtacgaaga
     3061 tgcaatgcct gcagcaaata tcgtctcatt gaggtgtgtc atttcaacca gcttgtcttt
     3121 gatgtgtgac gcgtttgcca caccattgta gtccgcaatg gctgtagcgg ctccaattaa
     3181 aacgtcgcca agaccggtct tgcatacata gcttcgacga tgatagcagg taaaacgttc
     3241 aactaacatc gaggcaaact catattcacc atccatgaat ataagatgat ttggaatgaa
     3301 aacatccttg aagattatca tagcctcttg accagaatac atggcattgc cagcgtcaat
     3361 gttacctcct tccatactcc ttgtatcaga tgactgtctg ccatagatat aggtgatacc
     3421 tggagcatca acagggattg ctccaataat tgcatagtct ttatcttcag acttcaatct
     3481 cattgtcggc atgactacta cccaatggga gttaataatg ccagtctggt gggcctttgc
     3541 accgcttatg tatatgcctt tgctgtcgcg ctttgtcacg tgcacaaaca tgtcagggtc
     3601 ttcctgctgg gaaggtgaca ggccacggtc acctttaacg tcagtcatgg caccagcgat
     3661 catcaagttt ccccgttgca gcatcttgac gtattccact aggcgattat gatactcagt
     3721 accatgcttt tggtcgattt catacgtagt tgaaaatagt gagttaattg cgtccatgcc
     3781 tacgcaacgc tggaagcagg taccagtgat ctgcccaagc ttcctttgca tcttattctg
     3841 gttaactaga tcctgcgcgc tttctgtaat gtgcaagaac ctgttgactt gcattcccgt
     3901 tagcgaagac tggggggcag caagctctgg ctcttctacc gcaaggtcat aggtctctgc
     3961 cactgcattt attgatggcc ggatcattgg atgatccact ggctctttta cgacctctcc
     4021 aaaaaggtat atcttgagat ttctacctct gaggctttca atatattctg ccccattctt
     4081 gatcgtcata taagacatat agcacttgaa tgtttattat actttctcaa gtgcaatata
     4141 ctaaagaaat agcatcattt gatgtagttt ttacacaaat ctgaaacgct agctagtttg
     4201 tgcctgaaac ctttcaccat caatcaacta tctcgcccga gccctttgta ttttttggca
     4261 ggattgaatt cttgtcaata tcaatttttg gtaatcttcc tcataacacc accttgagca
     4321 aatctacagc aaagatcccc tcgccgaacc agagttaagt gcaagtttct ttaagattca
     4381 attttatgaa attgctataa attagtattt ctatccggat ccgggtcttt taacaactgt
     4441 cttcattatt cctctaagct ttgttctact gctgactgac acctttgctg caaagatggc
     4501 gttgtcaatt ttaacagcct cttatcgtgg gcgagggttt cttattaaag tgtcattcga
     4561 tcaacattga atatcctcat acgacaataa ggttattgga tatatgtctg attggtcctg
     4621 tcaaagttcc tgacaacttg aaaatcccta ccaaactgtg actgcatcaa tgaccttaac
     4681 tcggggtata tagcggatgg accattcttt gccatcttgg ttgcagtatg atcattaccc
     4741 aacatccact tgcacaactc aaaagtcatc tggcgcacgg tgttctcatc tacgattcca
     4801 aggctgtgga ggtattcatg ggccagcacc atgaatatgt aagagttgta ctcttcggtt
     4861 gatttgacta tccttttcaa tgtagtcaag agatacgtgt tgaccactat tgcatttgat
     4921 cccagcatat ggtaggcgcc aaggttgctt ggcatcattt gcaatatcag gcttagtcct
     4981 gctcgatgca gtttgaactt ttgcaggact actgccttca ctaattcaaa tgcctcatca
     5041 aagtccttta ggcttgcaat cttctctata aactgcatct gctgttctgt gagcattatc
     5101 atttaatgca tactagagct gtataaagta tggtggtagt ggcttcgcaa tctttttaca
     5161 accagaatat ggccttactg atgtgggagc aaaagctaag caggtcaacc tacgcgagat
     5221 aatagccaac tatcctgatt ttcccaagca agggatacta ttccgcgaca taaatcctgt
     5281 tttcaaaagg aatgatgcct tgaactatat cgccaatgag ttttaccgca cttatagcaa
     5341 ggcaaaagta gacatggtgg ctggcatcga atcaagaggc tttatcatag cgacagcact
     5401 tgcactcaga ttcggcaggg gtattgtaat gattcgcaag gcaggcaagc tgccgggcag
     5461 aaccgtcaag aaatcatatg atattgaata cggcaccgcg ataatggagc ttcagcaaga
     5521 tgcaatagac aaaggcaacg gtatactgat cgctgacgac ctgatagcaa caggtgggac
     5581 tgcaatagca gccgcgcaaa tgatcaaaga gctgggcggt aaggtggctg gctttgcgtt
     5641 cataattgaa ctttcagact tgaagggtgc tgacaggttg cgcaagatgg gctataatgt
     5701 ggagtcattg gtgacttttg atggaaaata gcgtggatat cggcataatt gggggtactg
     5761 g
//
LOCUS       DASQTF010000029         8316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_467959,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000029 DASQTF010000000
VERSION     DASQTF010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467959"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..510
                     /locus_tag="VE308_00650"
     CDS             274..510
                     /locus_tag="VE308_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99508.1"
                     /translation="MVLDSEGIVTIVLVIGIFGGLVGGPILSLADYYAPPERRFWMFI
                     IPEFIGFGCLILMVVYGKSKKKRQKFQPNTQDNS"
     gene            728..1051
                     /locus_tag="VE308_00655"
     CDS             728..1051
                     /locus_tag="VE308_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZA99509.1"
                     /translation="MYDREAKRLLWYLFAGSRGGENRIRIIDLIKEQPHNINQLAEAL
                     GVDYKAVQHHIGVLEKNNMVIKVGEKYGVLYFVSNYLEANIGAFNEIRAAINKKGNLI
                     RSGKK"
     gene            1115..1396
                     /locus_tag="VE308_00660"
     CDS             1115..1396
                     /locus_tag="VE308_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99510.1"
                     /translation="MVNTVILVVLLAVYANIYRKTGAIFTIGLMVFAGMLMLHNIIAI
                     YGYFAMAPLYSDDLLPYFVGIHIAELAGLIALLKVTVWPEGIKSTPKAS"
     gene            complement(1418..1639)
                     /locus_tag="VE308_00665"
     CDS             complement(1418..1639)
                     /locus_tag="VE308_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99511.1"
                     /translation="MRPKEIGLQLCTCTWIEIMTGAGVVLYLILAMNDYILAGLAAGL
                     FAPLIVFHFVITLISKRNGLVRIEGMKNK"
     gene            complement(1682..2134)
                     /gene="msrB"
                     /locus_tag="VE308_00670"
     CDS             complement(1682..2134)
                     /gene="msrB"
                     /locus_tag="VE308_00670"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZA99512.1"
                     /translation="MSIVSDMKENRRSFIINNSIRQDIEKWKGKLTQEQYQVCMRKGT
                     ELPFTGKYWDCKEDGLYHCVCCGNELFNSETKFDSGTGWPSFWAPAKLQNLKFVSDKS
                     HGMRRMEVQCNNCGAHLGHVFDDGPRPTGKRYCINSASLEFEGRNQVG"
     gene            complement(2220..2873)
                     /locus_tag="VE308_00675"
     CDS             complement(2220..2873)
                     /locus_tag="VE308_00675"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HZA99513.1"
                     /translation="MNKKKAVIIVIAIAAIAIPLAVYTVSPLFIDTEINEPVPTVSNE
                     NNNGAGAIIEFNKFMEMSEQERKERGHQMRNEERDMIMIGAAQTNGTTVNEEMTTIEQ
                     EASTAYVGEFVGANDGIHNAEGQVKVLKSNDNTNNFLRLEDFKATNGPDLYVYLSTDK
                     SASDFVNLGRLKGNIGNQNYEIPEGTDLSRYPTVLIWCQAFSVLFGSTELEQVRSSA"
     gene            complement(2866..3111)
                     /locus_tag="VE308_00680"
     CDS             complement(2866..3111)
                     /locus_tag="VE308_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99514.1"
                     /translation="MVRKIWRRSGKIILILPCGQTLMKSNGWSCNQLALYVGRQPRII
                     ISSALNEVKNCSCSGVLFEFPSYAFDGIKPGMKIEYE"
     gene            complement(3236..3310)
                     /locus_tag="VE308_00685"
     tRNA            complement(3236..3310)
                     /locus_tag="VE308_00685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3273..3275),aa:Arg,seq:gcg)
     gene            complement(3434..3910)
                     /locus_tag="VE308_00690"
     CDS             complement(3434..3910)
                     /locus_tag="VE308_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99515.1"
                     /translation="MPRSKKQRTRLITLGGIAVIGALIAFMVFLQVEASRNNDFKRAF
                     EKIVIDTNALTQQYTAEEDKWLSRDNNTMIQIIDQYLPRYDELLDRAKALNTPEKYKS
                     AHDYLVSSIELERQSYQHFRNYLATTDQSEYTKSSEMISQSLEQSVNADAAIKAAG"
     gene            complement(4006..4164)
                     /locus_tag="VE308_00695"
     CDS             complement(4006..4164)
                     /locus_tag="VE308_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99516.1"
                     /translation="MQWDEGGTTLVFCSRHTLDYFDWDRTDREFVKMVATPKKSGKQD
                     RIALLEMR"
     gene            4557..5315
                     /locus_tag="VE308_00700"
     CDS             4557..5315
                     /locus_tag="VE308_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99517.1"
                     /translation="MADREYDAQEQDKGDEEQQQAGLSGAKRQIAQPPLNLLFNPSLL
                     IRKDVWSIDIARLLDMFLRLLSATGNKDLRICGLAAVSSSMIYRLKVESIFVLEKIAM
                     QKKGLNDSTNDQQQLPIPQLNPIELPFRIEPTYPVSVEDLLHMLENMIMELANPRSKR
                     KKQELELEPVETFNFDQYLIKFEKIIQDYENMIYDIVAADGMLMFKTLVKKMEPVEIA
                     RCFIAMLYLAMKRKIMLDYPQEDSDDIKMTLAQP"
     gene            5431..6030
                     /gene="scpB"
                     /locus_tag="VE308_00705"
     CDS             5431..6030
                     /gene="scpB"
                     /locus_tag="VE308_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZA99518.1"
                     /translation="MREPVLPEDEVAARIEAALYSAGRPLSLDELIRASGTNSKEKTH
                     RVVNDLAKKANSTFRAIEIAHLEDGKYVLQLRPEYTPLVRKFAQHPLLPSGALKTLSY
                     IAYEQPVTSKRLVQIRGSQVYSHIKVLEQLQFVEHENLGRLKVYRTTPKFQNYFGITD
                     LNAVKSKLVTATASTTTTTTTTTKASKQPQQRSTTKHNP"
     gene            6103..6543
                     /locus_tag="VE308_00710"
     CDS             6103..6543
                     /locus_tag="VE308_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HZA99519.1"
                     /translation="MQRKITYLTAMRKSIENLAGRKLTGGRKVAMRGRRKYEIDRYPN
                     EAIVGAGNTVTRRTRGNNNKVAFKTAEFANVIDQENKKITKSKILRVTKNPANRDYER
                     RGVISKGAIIEIENGAARVVSRPGQDGVVNAILVKATTTTTKTK"
     gene            6485..7081
                     /locus_tag="VE308_00715"
     CDS             6485..7081
                     /locus_tag="VE308_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99520.1"
                     /translation="MEWSTPFWSRQQQQQRRPSNNIIVTVKSKQGFLFFVDLSWDGPD
                     PKDRALCPYPVEFYCKPSPAESLNSLESGRLFFQVELGSGVRSVAGRLVNKAKSENLD
                     KLQDGASKIKQQTSISTTFVQDSQQPNRWLGELSIPASWLEGGTRTHERTGATSPKSG
                     TNPYSLEITYTLDNTNSCTARFSSEDAFHWQATSKGVM"
     gene            7148..7465
                     /locus_tag="VE308_00720"
     CDS             7148..7465
                     /locus_tag="VE308_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HZA99521.1"
                     /translation="MKDYEHVVLWLDYFNKNLRRRQGRKVKRDQAVFDPTVQELVEAT
                     RAAGFELSDQEINSDARFPRRSFVRSGYVMVSKKEGIKKSQIIDAVANKLLRRSLQKS
                     TTK"
     gene            7608..7913
                     /locus_tag="VE308_00725"
     CDS             7608..7913
                     /locus_tag="VE308_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HZA99522.1"
                     /translation="MPITQTGEVGEIIHLAKSGRLIVKLNAAGTETVKAGELLIDGSG
                     RRVGKVAELLGPVKTPYASVITMTDRTSRLVGSKVFGGGFAKKPRDRSRSFGRSKSR"
BASE COUNT         2533 a         1761 c         1762 g         2260 t
ORIGIN      
        1 gagatgttcc gaaaatatac cttgtgatgt attgactgca ataaaactgt attacagaaa
       61 tagtatgcaa ttgatcattt gtggtactgt tatggtactg ttactgttaa aaacctgggc
      121 acagggttcc ggcagaaaaa aattcaaaat ttggactcaa tgcaattaga aaagtgcggc
      181 taaaggagct tagtatttca acaataaaca ttgtgttagc taaaaaactt ttgccagtaa
      241 gcgcttacat ttaatgactg agttatcatg tgaatggtat tggacagtga aggaatagtt
      301 actattgtat tagttattgg aatttttggt ggtcttgtgg gcgggcctat cctaagccta
      361 gctgactatt atgcccctcc agaaaggaga ttttggatgt tcataatccc cgagttcata
      421 ggttttggtt gtcttatcct tatggtagtt tatggcaaat caaaaaagaa gaggcaaaaa
      481 tttcaaccaa atacacagga caactcctag ccagagtcac gccacatatt atagaaacga
      541 tgtaatagat atcttcaatt tattgttcgt gtcacaagtt gtaaccaaaa accgtttgtt
      601 tatctggtag caggattcta ccttgaacag acctcctgtg gccgaactca taacaagcag
      661 cagaacatga gttgatatga agcatccttt caagtttaaa tttctaataa ctctatcaca
      721 cagtcatatg tacgaccgag aagctaagcg cctgctgtgg tatctctttg caggctcgag
      781 gggcggcgaa aacagaatta ggataattga tctaatcaag gaacagcccc acaacatcaa
      841 ccagctcgca gaggctctcg gtgttgacta caaggcagtg cagcaccata ttggggtcct
      901 tgagaaaaat aatatggtaa tcaaggtagg cgaaaaatat ggagtacttt acttcgtctc
      961 aaactacctt gaagctaaca taggggcatt caacgaaatt cgagcggcaa ttaacaaaaa
     1021 gggcaatctg attagatctg gaaaaaagta aataagcgaa tctcgccgtc taatgattat
     1081 gactttactg atggatatca gctccatcat tagtatggtt aacacagtaa tcctcgttgt
     1141 gttacttgca gtgtatgcca acatttatcg caaaacagga gctatattca cgattgggct
     1201 gatggtattt gcaggcatgc tgatgcttca taatattatt gccatttatg gctactttgc
     1261 aatggctcca ctttattcgg atgatttgtt gccttacttt gtggggatac atatcgctga
     1321 gcttgcagga ttgatagctt tattgaaggt caccgtttgg ccggagggaa ttaaaagtac
     1381 tccaaaggcc tcatagacat ctgtaccctc ctagctttca tttattcttc atcccttcaa
     1441 ttctaacaag cccatttctc ttggaaataa gagtaattac aaaatggaat acaatcaagg
     1501 gcgcaaataa tcccgcagct aagccagcaa gaatatagtc attcatcgcc agtatgagat
     1561 atagcacgac ccccgcacct gtcattattt ctatccaagt gcaagtgcat agttgcagtc
     1621 caatctcttt cggtctcaat ttacattatc cttccacttt atagttgatg atctaatgtg
     1681 tctaaccaac ttggttcctt ccttcaaatt ctagcgaggc agaattgata caatatcttt
     1741 ttcctgttgg gcggggacca tcgtcaaaga catggccaag atgtgctcca cagttattac
     1801 attggacttc cattcttctc attccatggc ttttgtctga tacaaatttt aagttctgta
     1861 actttgcagg agcccaaaaa cttggccagc cggtgccaga atcaaatttt gtttcagaat
     1921 taaagagctc attaccacag cacacgcaat gataaagacc atcttctttg cagtcccagt
     1981 atttaccagt aaagggaagt tcagtccctt ttctcataca gacctgatac tgctcttggg
     2041 taagctttcc tttccatttc tctatatcct ggcggatact attattgata ataaaacttc
     2101 tcctgttttc tttcatatca ctcacgatag acattagccg tgatagatat tagaggatat
     2161 ttccagattc attccacctt gattccatat cgaaagctat attcaaatca cttcatttat
     2221 cacgcagaag acctcacttg ctctaattcg gtgcttccaa acagaactga aaatgcttga
     2281 caccatatta gaactgtggg atatcttgag aggtctgttc cttctggaat ttcgtaattt
     2341 tgattaccga tatttccttt tagtctgcca agattgacaa agtcagaggc acttttgtca
     2401 gttgaaagat aaacataaag atcaggtccg ttggtcgctt tgaaatcttc aagccttagg
     2461 aaattattag tattatcatt tgattttaaa actttgactt gaccttcagc attatgtatt
     2521 ccgtcatttg ctcctacaaa ttcaccaaca tatgctgttg acgcttcttg ttcaattgta
     2581 gtcatttcct cgttgacagt ggtaccatta gtctgagcag ctcctatcat tatcatatcc
     2641 ctttcttcat tgcgcatctg gtgacccctt tctttccttt cttgttcact catttccatg
     2701 aacttattga attctattat cgcacctgca ccattattgt tttcattgct tacagtcggc
     2761 acgggttcat ttatctctgt gtcgataaac aggggtgata cggtatatac tgcaagtgga
     2821 attgcaattg cagcaattgc gattacgata attactgctt tcttcttatt catactctat
     2881 tttcattcct ggctttattc catcaaaagc gtatgatgga aattcgaaaa gcacgcctga
     2941 gcaggagcaa ttttttacct catttaaagc tgatgatatt attatcctcg gctgtctacc
     3001 cacataaagg gccaattgat tacaactcca accatttgac ttcatcagtg tttgtccgca
     3061 agggagaatt aaaatgattt ttccagatct tctccagatc tttctcacca ttatagtttt
     3121 agtggaacaa aaatacggtt aacagctttt ttcactctat cagatgttat ggaatagcca
     3181 aattatatta gtggtaagaa ttgggtccat tttttgaacg tttttttaat tttagcgggc
     3241 ccagagggac tcgaaccctc gaccaactgc tccgcaggca gccattctat ccaagctgaa
     3301 ctatgggccc aatgttctct atctaacaac ttcgatgctg acgacggtcg taacagcaac
     3361 aattccttgt tatatatacc tcaattaaaa cctaggtgca tggacagacg aagacacaca
     3421 ttcacacagc tagctagcct gcggccttga tagcagcatc tgcattaacg ctctgttcaa
     3481 gcgactgtga gatcatctct gaggattttg tgtattcgct ctgatcagtt gtggcaagat
     3541 agttgcgaaa gtgttgatag ctttgccgtt ctaactcaat agagctgaca agatagtcat
     3601 gtgctgattt gtatttttca ggagtgttca aagccttggc tctatctaac agttcgtcat
     3661 acctgggcag atactggtca ataatttgga tcattgtatt gttgtccctt gacagccatt
     3721 tgtcctcttc tgcagtatac tgttgcgtca atgcatttgt atcaatcact attttttcaa
     3781 atgctctttt aaagtcatta ttccgtgaag cctccacttg gaggaatacc ataaaagcaa
     3841 ttagtgcgcc aattactgca atacctccaa gggtaattaa ccgagtcctc tgctttttgc
     3901 ttcttggcat tatatccctc tctcttgttc tctcagtctc tcttgcaaga atagaatacc
     3961 aaagaacaaa ataaatacgc tatgtcgtgt aaaaatttct ttttatcatc gcatctccaa
     4021 gagtgcaatt ctgtcttgct tgccgctttt tttcggtgtg gcaaccattt tgacaaattc
     4081 tctatctgtg cgatcccagt caaaataatc aagagtatga cggctgcaaa atacaagcgt
     4141 agtccctcct tcatcccact gcatggtaca gccgcaaggt agatgtcggt ctgccattcg
     4201 tattctaccg tcttatctaa tttaaaagtt tagagaattc ttctcttcta ccgcagaaag
     4261 cgcaatctcc ggggtgagaa ggtttgacat tttgtattta cccagacaaa gaaaaaacaa
     4321 ccaaactcgc aaacaggaaa aaaagagctg gtgagacaaa cgataagaat gaggagagaa
     4381 gaagaagaag aagaagaaat acaagtatgc aaaagcttga ggtgtgtagt cggcaaataa
     4441 ggactccgta gagagcgaga gaaataatga ctaagcttgg cgacaaagct actttacaaa
     4501 caagtattaa atggggctcg gccaatcgag acatatagcg agaaagagac agaaaattgg
     4561 ctgacaggga gtatgacgcc caggagcagg ataagggaga tgaggaacag cagcaagcag
     4621 gcttatcggg tgcaaaaagg caaattgcac agccaccgct caacttgctc ttcaatccgt
     4681 cgctccttat cagaaaagat gtctggagca tagacattgc gcggctgctg gatatgttcc
     4741 tacgattact cagcgccaca ggtaataaag atctgcgtat ttgcggattg gctgctgtct
     4801 cttcgtccat gatctaccgg ctaaaggtgg aaagcatttt tgtacttgag aaaatagcaa
     4861 tgcagaagaa aggccttaac gactcaacca atgatcaaca gcagttacct ataccacagc
     4921 taaacccaat cgagctacca ttcagaattg aaccaacata tccagtatcg gtagaagact
     4981 tgctccacat gctagaaaat atgataatgg agcttgcaaa tccccgttcg aaaagaaaga
     5041 aacaagaact cgagcttgag ccggttgaga cctttaattt tgatcaatat cttatcaagt
     5101 tcgagaagat aattcaagat tacgaaaaca tgatctatga cattgtagct gccgatggta
     5161 tgcttatgtt caagacactg gttaagaaga tggagccagt tgaaatagca aggtgtttta
     5221 tagctatgct gtaccttgca atgaagcgta agatcatgct tgattacccg caagaagatt
     5281 cagacgacat caagatgact ctcgctcagc cataacaaac gacaaaatag aaacaacaaa
     5341 agcgataaca aggatgatga taataacaac agcataggag caataaagta ataaatcgat
     5401 tatttagaat agagagagag atatacacag ttgcgcgagc cggtccttcc agaagacgaa
     5461 gttgccgcta gaatagaagc ggctttgtac tctgctggta gacctctgtc gctagatgag
     5521 ctgataaggg catcgggcac caactcgaaa gaaaagacgc atcgagtggt aaatgacctt
     5581 gcaaagaaag ctaactcaac ctttagagca atcgagatag ctcatcttga agacggcaag
     5641 tacgtactcc aactaagacc agaatacact ccgcttgtgc gcaaattcgc gcagcatcct
     5701 ctacttccat caggtgcact aaagacgctc tcctacattg catacgagca gccggtgaca
     5761 tcaaagaggc tagtgcagat aagagggagc caggtgtatt ctcacatcaa ggtacttgaa
     5821 caactccagt tcgtagagca tgaaaacctt ggccggctta aagtgtaccg gacgacgcct
     5881 aagttccaga actactttgg cataacagat ctcaatgccg taaaaagcaa gttagtgaca
     5941 gccactgcca gtaccacaac aacaacaaca acaacaacaa aagcaagcaa gcagccacag
     6001 cagcggtcaa ccactaaaca taacccctga tccgcagcag cggcagcagg cggcagcagt
     6061 agcagcacaa gccagcctcg gctaccagaa aagacttaat tagtgcagag aaagattacc
     6121 tacctcactg ctatgcgaaa atcgatagaa aacctcgctg gacgcaagct tactggtgga
     6181 cgcaaggttg ccatgagagg gagacgtaag tatgagattg ataggtatcc aaatgaagcc
     6241 atcgtcgggg ctggcaatac ggttacccga aggacaaggg gcaataacaa caaagtcgct
     6301 ttcaagactg ctgagttcgc caacgtaatt gatcaggaga acaaaaagat aaccaaatcc
     6361 aagatcttga gggtcactaa gaacccagca aatagggact atgaaagaag gggcgtaatc
     6421 agcaaggggg ccattattga gatagaaaat ggggcagcca gggttgtatc gaggccaggg
     6481 caagatggag tggtcaacgc cattctggtc aaggcaacaa caacaacaac gaagaccaag
     6541 taataacatt atagtaaccg ttaagagcaa acaagggttt cttttctttg tggacttatc
     6601 atgggatggt ccggatccaa aagatagagc tctctgtcca tatcctgtgg agttttattg
     6661 caagccttcc cccgccgagt cgctaaactc acttgaatcc ggacggctct tctttcaggt
     6721 tgaacttggg tcaggtgtaa gatcagtggc cggccggctc gttaacaaag ccaagtcgga
     6781 aaaccttgac aagttacagg atggggccag caagataaag caacagacat cgatctccac
     6841 cacattcgtg caggacagcc aacaacctaa cagatggcta ggcgaactga gcatcccagc
     6901 cagctggtta gaaggcggca caagaactca tgagaggaca ggagcgacta gccccaagag
     6961 tggcactaac ccatatagcc ttgaaataac atacacactt gacaatacca acagctgcac
     7021 cgcgcgtttt tcctccgagg acgcatttca ttggcaagca acgtccaaag gagttatgta
     7081 acgcacgaat atacgcaagc aatatacttt tataatttcg taaaatacgg aggatcgaga
     7141 aactctcttg aaagactacg aacacgtggt gctgtggctt gactacttta acaagaatct
     7201 caggaggcgg caaggcagga aggtgaagcg tgatcaggca gtctttgatc ctacggttca
     7261 ggagctagtc gaggctacca gagcggcagg cttcgaatta tcggatcaag agataaacag
     7321 cgatgcaagg ttccctcgca gatcattcgt caggtctggc tatgtaatgg tgtcaaaaaa
     7381 ggaaggtatc aaaaagtctc aaataattga cgcagtagcc aacaaattgc tgcgaaggag
     7441 cttgcaaaaa tctaccacga aataaaaatc gggtatagta caaaggtgta tatgcgtgca
     7501 tgttttcttt cttcctccca gctaatgcca ctgcacagag gctttattgt ttataaaaaa
     7561 taacaataat atctggcgca gaagcttatg atagaagtaa ctaacatatg ccaattaccc
     7621 aaacaggcga agtaggagaa atcatacatc ttgcaaaaag tggtaggtta atagtgaaac
     7681 ttaatgctgc tggcactgag acggtcaagg cgggcgagtt gctgattgat ggatcgggca
     7741 gaagggtcgg caaagtggca gagttattag gcccagtgaa gacgccatat gcttctgtta
     7801 taaccatgac agataggaca agcaggcttg taggatccaa agttttcggt ggcggttttg
     7861 ctaaaaagcc tcgagatcgg agtaggagct ttggtcgttc caaatcgaga taggtaaaaa
     7921 ataagaaaaa aaagttgtat ataggatatg ccttgaaagg aagctgaggc aaaaggcttt
     7981 tagatttaca tctataacct ccttctgcac ttgcggtttg agtacgtaaa ccctatggtt
     8041 gggcttgcaa tgttaatcat tggacttttc ttaatagtat ttggctattt tgaatatggg
     8101 attacgcctg tactagtcgg ccttgttctt attgcatact acaggaaata tctgtggcag
     8161 attctgggaa tctcacagct ctagctaacc atctcgatag cttttgaaag cacatctgct
     8221 cccatgagga ggcctagcct gccgcgtttg gcatagtttt cagtgaacct tgcacttcca
     8281 tcctgcttta ctctgctact ggaaaccagc aggggt
//
LOCUS       DASQTF010000030         4143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_5402,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000030 DASQTF010000000
VERSION     DASQTF010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5402"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VE308_00730"
     CDS             <1..616
                     /locus_tag="VE308_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99523.1"
                     /translation="RIDPALRKIIGVAIDDTVNIRKIADATVPVEKPASIEPEEAEAP
                     VAPPSESSENLGPGTQDTATTAAAAVPSAAGMFVLSGSCIDFEKERPKIMQFITTLES
                     ATRNQSKCKCYSDGKKALGWDFFLLELDEIFVDKLRDIYPDIEKQEAGSMEERFALWM
                     NKQLKKMVNMDFHLKLSDVPQEKINGFRLNPEHFRDKSDLEDLR"
     gene            complement(1095..2279)
                     /locus_tag="VE308_00735"
     CDS             complement(1095..2279)
                     /locus_tag="VE308_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HZA99524.1"
                     /translation="MEIIETMVEVGTIITIAIAVIIVGVIGFFIYKRKFRGGAFKRGP
                     GRYQRMTREGEPLEDYRGEGGGLISRSSVRIVIPVIIAFILVIVILAASIKIVEAGHR
                     GVLLNFGAVDTSASLSEGIHFVVPFRDNVVQTEVRTQKTVENAASASRDLQDVSTQVA
                     VNYHINPDTAQVLFQQLGLDYANRVIAPAIQESVKQVTARFNAENLITNRETVKAEIE
                     NQIKQRLAAYNIDVEALSITEFQFSEQFRRAVESKVEAEQRALQATNDLRRIEIEAQQ
                     AEARAIGEREANIARAEGVRQAAVLQAQGEAEAIQIVEAQLRENPRYLEWLKTQRWDG
                     VLPLVTGGAEGATPFIEIPSSREGTVQEAGTAEQGSNGTATSTPTTAAAAAAGNSTQA
                     AR"
     gene            complement(2781..3107)
                     /locus_tag="VE308_00740"
     CDS             complement(2781..3107)
                     /locus_tag="VE308_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99525.1"
                     /translation="MDNNTKRFMIETLMWIGIYFGIAFIISRLLPFPYSLIVIIVAVI
                     GLGFYRRRSQFRKAGQASSPGTSYFGNMFGSQKGINYYCMNCGTKHNQISCPNCGSKL
                     KKPGFS"
BASE COUNT         1123 a          841 c          873 g         1306 t
ORIGIN      
        1 aaggattgac cctgccttga ggaagataat aggtgttgct attgacgaca ctgttaacat
       61 aagaaagata gcagacgcta ctgtaccagt cgagaaaccc gcaagcatag aacctgagga
      121 agccgaagca ccggtggctc caccgtcgga atctagtgag aatctgggac ccgggactca
      181 agacacggca acaacagcag cagccgccgt cccttctgct gctggaatgt ttgtcctgtc
      241 aggttcgtgt atagactttg aaaaggagag acccaagata atgcaattta taactaccct
      301 agaatctgca acgcgtaacc aaagcaaatg caaatgctat tctgacggca agaaagcctt
      361 aggatgggat ttctttctgc ttgagttgga cgaaatattt gtagacaagt tgcgtgacat
      421 atatccagat attgagaagc aagaggccgg tagcatggag gagcgctttg cactttggat
      481 gaacaagcag ctaaagaaga tggtcaatat ggattttcat ctaaagttga gcgatgttcc
      541 tcaggaaaag atcaatgggt tcagacttaa tcctgaacac tttagagaca agtccgactt
      601 ggaagatctg cgctgactgc aatctttggg aacaggctac agcaaatcga tgcagggaag
      661 gagaattagt gagaatatat tacatcatca tcagtttaat catatggagg ctataatcga
      721 atggatttta ctcaacgctg gcttttgatg atggcaattg ctacaggcct atttgctatt
      781 gcgctgctcc tcctacgagt caatattcgg acacttgcta tccttcttgg cattggatta
      841 ccccttgtta cactttggct gtatatggat gctaagcatt ttagtgtgca aggaagaaat
      901 acttcatcaa aacaggcctg tgtatgtcct gtctgcaaac accaggatgc aagaatgtgt
      961 tttgaggaga aatgttcatg ctgcctgatt acaaaagatg aaaaagtgat agggcattcg
     1021 agcagtccat tgcagtagag gagggattaa aaaataagtc tgctgctttt actttgccct
     1081 agtatgctgt cgttttaccg agctgcttgt gttgaatttc ctgctgctgc tgccgctgcc
     1141 gttgttggtg ttgaagtagc ggtgccatta gaaccttgtt ctgctgttcc agcttcttga
     1201 acggtgcctt ctcttgatga tgggatttct atgaatggcg ttgctccttc agcgccgcct
     1261 gttacaagag gcaatacgcc atcccacctt tgagtcttga gccattctag atagcgcgga
     1321 ttttctctca gctgcgcctc aactatttgt atcgcctctg cctctccttg tgcctgtagt
     1381 acggctgctt gccttactcc ttctgcgcga gcaatattgg cttccctctc tcctatagcc
     1441 cttgcttctg cttgctgcgc ctctatctcg attctcctaa ggtcgttggt agcctgaagt
     1501 gctctctgtt ctgcttccac ttttgattca actgctcttc taaactgctc tgagaactga
     1561 aattctgtga ttgatagcgc ctctacatca atgttgtaag ctgctagtct ttgctttatc
     1621 tggttttcaa tttcagcctt tacagtctct ctatttgtaa tcagattctc tgcattgaat
     1681 ctagcagtta cttgctttac ggattcctgt attgcagggg caatgactct attggcataa
     1741 tcaaggccga gctgctggaa taagacttga gcagtatccg ggtttatgtg gtagttaact
     1801 gcgacctgtg tgctgacatc ttggaggtcc cttgatgcag aagctgcgtt ttcaactgtt
     1861 ttttgtgttc taacctccgt ttggactacg ttgtctctaa atggcactac aaagtgaatg
     1921 ccttcgctga gagatgctga ggtgtcgacg gccccaaagt ttaatagaac tcctctgtgg
     1981 ccagcttcta cgattttgat gctagcagct agtattacaa taaccaggat gaaggctatg
     2041 ataaccggta ttacgattct cactgaagat ctagatatta atcctccacc ttctcctcta
     2101 tagtcttcaa gtggctctcc ttcacgggtc attctttggt agcgtccagg accgcgtttg
     2161 aaggctcctc ctctaaattt tcgtttgtat atgaaaaagc ctatgactcc aacgataatt
     2221 actgcaatag caatcgtgat tatagtgcca acttctacca ttgtctcaat gatctccatt
     2281 tgtcaaatct cgaacttgcg tacattattt ttgggatttg catgtactaa attaatacaa
     2341 tatgacaata tgaagtttta tctgtcagac attctcattt aaaattgaat gtgataatat
     2401 tctataagat ctttctacca taacagtctg gttgctgagc cagtcaaagt gtggtctata
     2461 ttttgggctg ccatgggttc tactaaaggg ttcaaaactt tttcatcact tctatatttc
     2521 ttcttccttt agaaccattc tcttggttca gatgcggtga tagcgggttc gctctcttaa
     2581 aggaccacct gctaggatgg caagcgttaa atttagggcg ggaaatgcga atcccatacg
     2641 ctctatcatc acaggtattt ttcatatttc tatgatcttt gagatcatgg tgctttgttg
     2701 atgaagagct tcagaatagg gtgatgtata cacctttttt caataacctc cacatgctct
     2761 gcccagtaga tactactctt tcatgagaat cctggtttct ttagttttga tccacaattt
     2821 gggcatgaga tctgattatg ttttgttccg caattcatgc aatagtaatt tatgcccttt
     2881 tgagagccaa acatgttgcc aaagtatgaa gtacccggag aggaagcttg gccggctttt
     2941 cgaaattgac ttcttcttct gtaaaatcca aggcctatca cggcgacgat gatgactatc
     3001 agagaataag gaaatggcaa tagtctagaa atgataaatg ctatcccaaa gtaaatgcca
     3061 atccacatca atgtttcaat catgaagcgt tttgtattat tgtccatttt taggaatcaa
     3121 cactatctca taatggttat tgatattatt ttaaagtaat attgacaatt aactttttaa
     3181 ttgcttcagt caaaagataa ttatcaatat atgtgagtaa gaaccaccaa gcagtataga
     3241 agaagggatg ggctctgagg ctcttgctat gtggatatat ttctaatgct aacctcataa
     3301 tcctctagtt tctagtatag aaacaagcaa atctatcttt tgagaatcaa aaagctgctt
     3361 tggtctatgc atttctaatc ataatatcct tactgggatt cacaatatcc ccttgcacgt
     3421 agcttgcatg taggttataa tcccgcatct tcagtcgaaa ttcttaagat ggtactaagc
     3481 ctattgggca aggtctaagg ttatttagtc caacttgggc atatgtactt gctctgagaa
     3541 gtgtttagac ttttctgtaa atgacgggat tgttctttga atttatgagc acataggatg
     3601 tatcttgagc cagccattat ttctgttgac atatttgtgg aatacaatgg tttttgtaag
     3661 tttggctcct atcagattca agaagaagaa gaagatatcc tttggaccaa gtaaaagcaa
     3721 ctatttgtta ggcggacctg atttaacatt ccgagcaaga aaaatcaaaa atagttcagc
     3781 aatgaaaaat gtaaagtcat agaagccttg ctaggataat gagcaatctc tttatgttca
     3841 gctatagtgg caaaggaact attgcatata tcaacacggt ccatagtgat ttaggagcgt
     3901 caaattgcat gataataaat gcatcctgca gccgatgtaa tacacacggt ttttgaatgg
     3961 ttagttgtaa aaactcaaac agaactctgg aatggttgtg cgagtgaaac ctctgtgatc
     4021 cattcgcact ttatgttgac ctttgcatga tgtttctcta ttgcgtcaat atctgggccc
     4081 tctagcaagc aaaaacaaat ctttgcatcc ttgttgtaca ataggttgac atgacgaaca
     4141 cca
//
LOCUS       DASQTF010000031         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_930978,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000031 DASQTF010000000
VERSION     DASQTF010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930978"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VE308_00745"
     CDS             complement(<1..1395)
                     /locus_tag="VE308_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZA99526.1"
                     /translation="MPKFKSTQDILRIIGNKDQIRNFGVIAHVDHGKTTMSDSLLAAS
                     GIISPSVAGQALALDSMKLEQNRQMTIRGANVTLFYEAEGKEYVINMIDTPGHIDFTG
                     RVTRALRAIDGVVVVSDSVEGIMTQTETVTRQALEERVRPVLYINKIDRLVKELKLNP
                     DEMQRWLINIINHFNQLIDIYAEPEIKEKWKVSIQGNSVAFGSAKDRWGFNFNMAQKK
                     GIKFKDVYDAYMSNDPNAIKALAERAPLSDAVLGMVVQHHPPPHVAQRYRIPKIWPGE
                     LNSDIGKALVECNDKGPALMMVTTINVDPQAGRVATGRLYSGTIKDGDEVYLIDAKRP
                     GRVQSVNIYMGNTREIVGFLPAGNIPALLGLDYAVAGETLSTVKGQVAFESIKYVSEP
                     VVTIAVEPKHPKDLPKLVEALRRVTVEDPNLIVKINEETGETLMAGMGVLHLEIATSL
                     LQEAGLDIVTTQPLI"
     gene            1505..2335
                     /locus_tag="VE308_00750"
     CDS             1505..2335
                     /locus_tag="VE308_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HZA99527.1"
                     /translation="MDITLGHTPDADDAFMFYALASGKLGSIFVIKHVIQDIETLNQC
                     ALKHELDVTAVSAHAYAYLKDYVILKSGGSFGLNYGPLVITRKENMMSPTQLQNAIIA
                     IPGKLTSANLLLELAIGKFKEKVVPFETVPDAVARGNVDAGLVIHEAQISYDKTKFAS
                     ALDLGRWWSENTGNLPVPLGINVASTRTISMDQIRQFDNLFTKSIKYGLDNIDAALEY
                     AMQYGRGQPKETVKKFVKMYVNNLTLDMGHEGKKALEKMFEMARERKIVDLDIKIEVM
                     "
     gene            2707..3369
                     /locus_tag="VE308_00755"
     CDS             2707..3369
                     /locus_tag="VE308_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99528.1"
                     /translation="MSNQRLPNEEGIERVSDVEESLNPHNVQGLQEEVPTTTTLPPNE
                     EEENAENVLASTIETDEEKSSSSSSVADQETQEEAVTEEGKRAAEEQQITQLDTANEQ
                     QQQQQVIPTMSPEQVLLLQVLRRAKRQQELIIEIQKNLKSLTNIQKGIEKTIEQVKQL
                     QSVVKGSQKQIIQIQRQISVVERAQEKEFAKLRTQKRGGGALISIKGKAAKNRGKRKK
                     IR"
     gene            complement(3791..>3982)
                     /locus_tag="VE308_00760"
     CDS             complement(3791..>3982)
                     /locus_tag="VE308_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99529.1"
                     /translation="KILKKTLKGIGVKEIYVGKLEDNAIHLTCFAKWKTSQVDKKKAE
                     IEKKIPKMRVIQIQPMTRI"
BASE COUNT         1195 a          830 c          849 g         1108 t
ORIGIN      
        1 gatcagaggc tgggtcgtaa cgatgtcaag acctgcctcc tgcaaaagtg aagtagcaat
       61 ttctaggtga agtacaccca taccagccat cagggtttcg ccggtttctt cgtttatctt
      121 gactatgagg ttagggtctt ccactgtcac cctgcgcaaa gcttcgacta acttgggtag
      181 atccttgggg tgctttggct ctactgcaat agtgactaca ggctctgaga cgtatttaat
      241 agattcaaat gcaacttgtc cctttacagt cgaaagggtt tcgcctgcaa ctgcgtagtc
      301 gagcccaagc agcgctggaa tgttgcctgc cggcagaaag cctacaatct ctcgtgtatt
      361 acccatgtat atgttgacag attggaccct gcctggcctc tttgcgtcta taaggtacac
      421 ttcgtcaccg tccttgatag tgccggagta aagtctcccc gtagcgactc taccagcttg
      481 cgggtcgaca ttaatcgtag tgaccatcat gagtgcaggt cccttgtcat tacattctac
      541 tagcgccttg ccaatgtcag agttgagctc accaggccaa atcttgggga tcctatatct
      601 ttgtgcaaca tgaggcggcg gatggtgctg tacaaccatt ccaagtactg catctgacaa
      661 tggtgctctt tctgcaagtg ccttaatggc atttggatcg ttgctcatgt aagcgtcata
      721 aacatccttg aacttgatac ccttcttttg ggccatgttg aagttaaatc cccacctgtc
      781 cttggcagag ccgaaggcta cgctgttgcc ctgaatactt accttccact tttccttgat
      841 ttctggctcg gcgtaaatgt caatcagctg gttaaagtgg ttaatgatat tgatcaacca
      901 cctttgcatc tcgtcaggat tcagctttaa ctccttgacg agtctgtcta tcttgttaat
      961 gtaaagcacg ggccgtacac gctcttctag ggcctgtctt gtaacagtct ctgtctgagt
     1021 cattatgcct tccacggagt cggaaacaac gaccacgcca tcgatcgcac gaagtgcacg
     1081 ggttaccctg ccggtaaagt cgatgtgacc gggggtatct atcatgttaa taacgtattc
     1141 cttgccttca gcttcgtaaa atagcgtgac atttgctcct ctaattgtca tttggcggtt
     1201 ctgctcaagt ttcatagaat caagcgccag agcctgacct gctacagatg gagaaatgat
     1261 gcccgatgct gccagcaggc tatcactcat ggttgtctta ccgtggtcaa cgtgggcaat
     1321 gacgccaaag ttgcgtatct gatccttatt accaattatt ctcaaaatat cttgagttga
     1381 cttaaacttt ggcatttgat caagccaaaa aagtttggtg caggattatt aaatgttgtt
     1441 ccggccaggt gacaaatagg attccacggt ttaatagtga cagaagcgac caaagaaatg
     1501 acaaatggat ataacccttg gccatacacc cgatgcagat gatgctttca tgttctatgc
     1561 acttgcatca ggcaagctcg gctccatctt tgtgattaag catgtcatac aggatattga
     1621 aaccttgaac caatgtgctc tgaaacatga actggacgtg acagcagtgt cagctcacgc
     1681 ctacgcctat ctaaaggatt atgttatact aaagagtgga ggcagctttg ggctcaatta
     1741 cggcccactt gtcataacaa gaaaggaaaa catgatgtcc cccactcaac tgcaaaacgc
     1801 aataatcgca attccgggca aactcacttc tgccaatctg ctcttggaac tggcaattgg
     1861 taagttcaaa gaaaaagtgg taccttttga gacagtaccg gatgctgtcg ccagaggaaa
     1921 cgtagacgca gggcttgtca tacacgaggc tcagatatca tatgacaaga ccaaatttgc
     1981 tagcgccctc gatcttggcc gttggtggtc cgagaatact ggcaacctgc ctgttccgct
     2041 tggcatcaat gttgcaagca ctcgaactat tagcatggat caaataaggc aatttgataa
     2101 cctgtttaca aaatctatca agtacggcct tgacaatatt gacgctgcac ttgaatatgc
     2161 gatgcagtat ggcagaggac agccaaaaga aacggtaaaa aaatttgtca agatgtatgt
     2221 taacaacttg accctagata tgggacatga aggcaagaaa gcacttgaga aaatgtttga
     2281 aatggcacgg gagcgaaaaa tagtagactt ggatatcaaa atcgaagtta tgtgatataa
     2341 gatatttttg ttgacaaatt acttgactcc ttttgtcaga tctatgattg ggaacatctt
     2401 gctatatttg atcttgtgac tacttgtatt gaggtgcgta tctcatgagc gagagtgatt
     2461 atgggcaggt ccttggaact gtgcacatgg actttaaagg catgttgtcc acaatatatc
     2521 tgtgattaga aaagccattc taatatggat tatctcaagg attgctaaat ccgtcatgag
     2581 gagagcattc aaatccagaa gaaagtatca ataacttaaa tttaatattc ataatacacg
     2641 tctactatct accaaaaaca attttagtat cttatttata ataattctat aggttgtgta
     2701 taaaatatgt ccaaccagcg cttgccaaat gaagaaggga tagaaagagt tagtgatgtg
     2761 gaggaatcac ttaatcccca caacgttcaa ggactgcaag aagaagtacc aactaccact
     2821 acgttaccac caaacgagga agaagaaaat gcagagaatg tattagcctc aaccatcgaa
     2881 acagatgaag aaaaatcatc ctcatcttct tcagttgcag accaagagac acaagaagaa
     2941 gctgttacag aagagggcaa acgagctgcc gaagaacaac agattacaca attggataca
     3001 gccaatgaac aacaacagca gcaacaagta attcccacaa tgtcgcctga gcaggtattg
     3061 ttattgcaag tgcttagacg agctaaacgt cagcaagaac taatcatcga aatacaaaag
     3121 aatctgaagt cgttaacaaa tatccaaaaa ggtatagaaa aaactataga gcaagtaaag
     3181 cagctacaat ctgtagttaa aggttctcaa aaacagatta tacagataca gcgccagatc
     3241 tcagtagtag agcgagcaca ggaaaaagaa tttgcaaagt tgagaacaca gaaacgagga
     3301 ggaggggcac ttatatcaat taagggcaag gctgctaaaa atagaggaaa aagaaagaag
     3361 ataagataaa taaaatagca gagacaggtt aaaaagaatg atttacatca cctctatcct
     3421 atcacatcca tgcaatactc atcagcaata gactgactag tcaatatagc aatcagaatg
     3481 tcgacgagtc ggaagttatg taatgatagc caatgaagga tcatattttc tgctctttta
     3541 ttagaaaagt gcagtgtatg tgggttcctt ctcgtgatgc ttcgtcaatg agtgcacgaa
     3601 attgcgaagg tacatttaac caagataact tgggcgaaga agtagagaca tgtttgttca
     3661 gtacttcatt gctcctggta atgggctctt gttgttttta tccctgtttg tttaggattt
     3721 gcaacatttc taacattaat taaaaaagcc gcctttattg aggtattagc cttgatattt
     3781 ataaaccaaa ttatatgcgg gtcataggtt gtatttgaat gaccctcatc tttggaattt
     3841 tcttttctat ttctgccttc tttttatcca cctgggaagt cttccacttt gcaaaacatg
     3901 tgaggtggat tgcattatct tctagtttac caacatatat ttccttaacc cctattcctt
     3961 ttagtgtttt ttttagaatt tt
//
LOCUS       DASQTF010000032         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_98824,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000032 DASQTF010000000
VERSION     DASQTF010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98824"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..916)
                     /locus_tag="VE308_00765"
     CDS             complement(239..916)
                     /locus_tag="VE308_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Snf7 family protein"
                     /protein_id="HZA99530.1"
                     /translation="MTSFSSNWIKDNKPTMSEMIKQNVGQAQPLKPRIELAKKKIQAQ
                     NQKLETILEKLRGKERSLFNQTVYALQKHDTQQGKMISNEIAQVRKTTKMISQFKIAL
                     EQIQLRLESTIDLGDVMVAIGPAMGALTKVRTGIGGVMPEVDRELGEINGVFSDIMMS
                     AGSLGHTSFAFDASGEEVDRILAEAGAVAEQRMSDSFPDVPIGSYSSSSSSSSSSIRT
                     SAGEHQQ"
     gene            1056..1517
                     /locus_tag="VE308_00770"
     CDS             1056..1517
                     /locus_tag="VE308_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99531.1"
                     /translation="MGRTKVTKSEDSSQEEGLPKWAEDEIRSAQFGQPEVITRTGYFL
                     DIYESDYKVDIQVYEQMPDGRTIVEGLDVPKSMKISDFMKGFVYEYKIKVFMASLSAK
                     VAELLKTKFGLDMKAIFRFELQELQLMDVESDLPAASSLSSSSGEDDNDDT"
     gene            complement(2138..>2995)
                     /locus_tag="VE308_00775"
     CDS             complement(2138..>2995)
                     /locus_tag="VE308_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZA99532.1"
                     /translation="ARLAGEFVTQTTPSYVGGELVRIAWLSRNGVPPGRAAWVTTMEI
                     IADVFVGSVLAFIAGALALYRGGSVIGLVVILVTIPTLAFWLGLILFSAKRTLRLPSF
                     SQKIAEKFLSKKRAENLINAANTAFADLCNMSREIFNSKKSIGTFAVGIAITVIAFLF
                     QGMSFMVLANAVEPRIGLFDSLMATSASTAVANLPITIGGSGLAELGIWAYVANLSGI
                     PNLEDVVKDSQLNVIIAWRIATYHVPLVIMWIALMKITVYRVPAPGIRQPIPGNEEKK
                     PENDSSSTI"
BASE COUNT          801 a          695 c          656 g          843 t
ORIGIN      
        1 ccggcaccgg catcatagac caccgacaat atttgtccat attgttataa tgataaaccc
       61 tactagatac ccggtttaat gacacaatgt cccccatgcg tatgagataa atgcaagagg
      121 acacagttgc cggcatttca tattataagg caatatcgct ggtctcactt ctatagggat
      181 ttgaagtatt tcaaaactga atattcaaag aaaaaagaaa aaagagatat agttgattct
      241 actgttgatg ctcgcctgct gatgtccgga tgctgctgct gctgctgctg ctgctgctgg
      301 agtatgatcc aattgggacg tcggggaagc tgtcgctcat cctttgctct gccactgcgc
      361 ctgcctctgc aagtatcctg tctacttctt caccacttgc gtcgaaggcg aaggaggtgt
      421 gaccaagaga tcctgcgctc atcatgatgt cgctgaacac accattgatt tctccaagtt
      481 ctctatctac ttctggcatt actcctccaa ttcctgttct cacttttgtc aatgcgccca
      541 tcgcaggacc gattgccacc ataacatctc caagatcgat agtggattcc aacctgagct
      601 gtatctgttc aagcgcaatc ttgaactgtg agatcatctt ggttgtcttt ctcacttggg
      661 cgatctcatt tgagatcatc ttgccctgct gagtgtcatg tttctgaaga gcataaaccg
      721 tttggttgaa gagagacctt tctttgcctc gtagtttctc cagaattgtc tctagctttt
      781 ggttctgtgc ctgaatcttc ttcttggcaa gctcgattct cggtttaagc ggttgtgctt
      841 ggccaacgtt ctgctttatc atctcagaca ttgtaggttt attgtccttt atccagttgc
      901 tgctgaagct tgtcatagca actagccagc gataatagcg ttaaatagaa acgtcataat
      961 tgaataacca tctggttatt ttttgtagac atagctgcct gcacaactag gttggaaaag
     1021 ctattaaatg caatgacaca tgcaagacta acattatggg aaggacaaag gtgacaaaga
     1081 gcgaagatag ctctcaggaa gagggacttc ccaaatgggc agaagacgag ataagatctg
     1141 cccagtttgg ccagcccgaa gtaataacaa gaaccggcta cttccttgac atttacgaaa
     1201 gtgattacaa ggtcgatatc caggtctacg aacagatgcc tgatggcaga acaatcgttg
     1261 agggcctaga tgtgcccaaa tcaatgaaga tatcggattt tatgaagggc tttgtatacg
     1321 agtacaagat caaagtgttc atggctagct tgagcgccaa agtagccgag ctacttaaga
     1381 cgaagtttgg ccttgacatg aaggcaatat ttcgttttga gttgcaggag ctgcaactaa
     1441 tggatgtcga atctgaccta cccgctgcct cctcattatc atcttcctct ggagaagatg
     1501 ataacgatga cacataatca ttgcaggcgc tggcactata tattgtccac ttggttatac
     1561 tacccacgct agcatgtagt taaaccgtac gctacccaat cttgaatgaa gaggactatt
     1621 atttccaggc gggacgagtt ttgcgcagca agtgtcagta ctctctttag gtcaggcacg
     1681 aagtcggttt aatttcttgc attcttttat catctccaag cattcagggc tgaacttgcg
     1741 acttcgcatc ttggtgggat taacttggca tctactatgt gagtcgaatt atttgtaggc
     1801 atcgaggaaa gaaagattac aggtagcaga ccatgtgagg atattttgtc gtgtgccatt
     1861 gatcaatttc tcgtaggcaa agcctgttga tgctttggaa gaagagatcc attgcaaata
     1921 tatgcaaatt gcactacagg cttcaggcaa cttcaatttt gtttgctaga agagataatt
     1981 tcttacttac ctcaaatatg agaacgaaca aaattatgca aaccttgctt tgtaaaatcc
     2041 aaagcaactt gcattataat accgtaggaa gcctttatga gaactatttt cttgcaaaga
     2101 aacctcatgt gttcttcttt atggctagcc cgccctatca tatagtgctg ctactatcat
     2161 tctccggctt cttttcttca tttccaggga ttggctgtcg gatgcctgga gccggaaccc
     2221 tgtatacagt gatcttcata agagcgatcc acataatgac aagtggcacg tgataagtag
     2281 ctatcctcca cgcaattatc acgttcagct gcgaatcttt gaccacgtcc tcaaggtttg
     2341 gtatgccact cagatttgca acgtatgccc aaatgccaag ttcggcaagt cctgagcccc
     2401 ctatcgttat cggcaggttt gccaccgccg ttgacgctga cgtagccatc aatgagtcaa
     2461 ataagccgat tcggggctca acggcattgg caagcaccat gaatgacatg ccctgaaaga
     2521 gaaatgcaat aacggttatt gctattccaa ccgcaaatgt gccaatcgac tttttggagt
     2581 tgaaaatttc ccggctcata ttacataggt ctgcaaaagc cgtgttagct gcattgatca
     2641 ggttctcagc cctttttttt gacaggaact tttctgctat cttctgagag aatgacggga
     2701 ggcgaagagt gcgtttggca gaaaaaagga taagccctaa ccagaaggct aaggtcggga
     2761 tcgttaccag aattacgacc aatccaatca cgcttccgcc tcggtaaagg gccagcgcac
     2821 cagctataaa tgccagcacc gaccctacaa atacatctgc aattatctcc atggtcgtta
     2881 cccatgcggc cctgcctggc ggaacgccat ttcttgacag ccatgctatc ctgaccaatt
     2941 ctccgcctac ataagatgga gtagtctgcg taacgaactc gccggccagc ctagc
//
LOCUS       DASQTF010000033        11584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_654635,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000033 DASQTF010000000
VERSION     DASQTF010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654635"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /gene="cbiE"
                     /locus_tag="VE308_00780"
     CDS             <1..687
                     /gene="cbiE"
                     /locus_tag="VE308_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HZA99533.1"
                     /translation="GPGSPKYLTDAAKEAIAKSHYIIGYKYPLSTIEGVIDKSRQQVF
                     QVTMQTQEGVYQDIYTKMKDGEYCTVPFTGDANFSESEVVDRLLEIFGSDNVEVIPGI
                     SSIQVAAAKAHVSTDKAFIVTFHVTDDIEQKKQEMLQAVRQGRSVILLPRPWPRDRSR
                     HFMHSEIAKFLRKSGIDTTKLKVWVFEHLTTEKETTFRGTAVDLEGKEFSDLSAMVVD
                     QTKRQTYLNF"
     gene            complement(1205..1999)
                     /locus_tag="VE308_00785"
     CDS             complement(1205..1999)
                     /locus_tag="VE308_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99534.1"
                     /translation="MIASIIAATTMITAMSWTTKSIGSLPIAKNCGKTVFPALVVVAE
                     WFTAIVMFNIHKKTKLGRRGGRMIKLIIQYHFQPFLLGFKINKWRLIYLAFGAAAFLI
                     AYSIGGAVPMSEEEAETLMREFSKQIEGIDQNGIFLNNVRITLIMFVPAVGSAFGAFS
                     GFATGSIFSALASQSPMLGEVSPLAILITPFGIMEVFVYGLAMSRSAMLIYYLLKRIP
                     WRKYIIPTLIELGIATGILFVAAVIEWQIIERLGGLDASIAVDPVE"
     gene            1968..3146
                     /locus_tag="VE308_00790"
     CDS             1968..3146
                     /locus_tag="VE308_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZA99535.1"
                     /translation="MVVAAIMLAITYKLKQPMVIGYIIAGMIIGPYTPPFNLVRSPET
                     LNLFAELGIIMLLFVTGTEFPIAKLRSVGKISVATALAEVLGTLLIVFFIAQSLGFQF
                     YDSLFLALALSITSTVITIRILEDVGLIRDKSSTLILGMLIVEDIIAISALGILQSFV
                     ATGGNMSILNIAIVLGIVGGFIGSVIIVGSKFVPRIIDRAGKTNDYALLLITILSIAF
                     GLSFLANGLGMSVVVGAFLAGVLVAESRSAAVARIITIPLRDMFAALFFISIGALMNV
                     SLIPAFIIPAIILILTSFASKLLIVTGILVRAGYDSTTALRAGFGISATKGEVSLIVA
                     KGGQDVNAITPFILPLLGVVTIVTTFIGPFIIKVGSRFKLTESTEAEDAKKEKEEEES
                     "
     gene            3538..5736
                     /gene="pstS"
                     /locus_tag="VE308_00795"
     CDS             3538..5736
                     /gene="pstS"
                     /locus_tag="VE308_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZA99536.1"
                     /translation="MSAFFTGIFMPLGNSNAEQRIDLNGAGATFPFPLIDAWRVEYQK
                     IKPEININYQSIGSGGGVKQFSEKIVDFGATDAPLTSSEMEKAREAVHIPETIGSIIV
                     SYNIPEVPDKGLKLTGPILAGIYLGKITKWNDPQIQKLNPDLFLPAQNIVVAHRSDGS
                     GTTFVWTDYLSKVSNEWKAQVGKGKSVQWPTGIGAPGNEGVASTIKSTPYTIGYIELS
                     YALTTRMPYAYLENQEGNFVEPSIDSVRAAVASTAKLLPRGDESWEQISTTDAPGKDS
                     YPISSFSYILLYKELSTNPRIDSMDKARALVDFVSWVITDGQQFADDLGYIALPDDVV
                     ILNQDTLSSLTYKGQPVLTTESETDPASPEILVPFVAAAAVILPIVAAVGIIYVRRKN
                     RGSLSKEVTKIGAKKESVHDRLSPFLLKSTPTGDKIFRFIVIGAASYTLVLLGLVAIS
                     IFSGSAEVFYSEGVIGFITGTDWNAVEGRESYGALPYVVGTLISSGIAMTIGVPISLG
                     IAIFIVEIAPYRMSIPLSFVIELLAAVPSIIYGLWALFVFRFWIRDFIEVPLNHAFGE
                     TIPIFAGTPFGLDVFTAGIVLAIMIIPTVSSISREVIRAVPNIQREAAYSLGATRLEM
                     IKTAIFPYAKSGLFGAAILGLGRAVGETILVTMIIGNAIGASAIPNSLFSPSQTLASL
                     IANEFNEAVTSLHVSALIGLGVVLFLLTIGINIGARLLVSQIAKVSPGRE"
     gene            5747..6694
                     /gene="pstA"
                     /locus_tag="VE308_00800"
     CDS             5747..6694
                     /gene="pstA"
                     /locus_tag="VE308_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZA99537.1"
                     /translation="MAEQNERLTETSANDGGSRRRRIEIQQMMDKQSIRRRLFDKIAT
                     GVVMACVIAAIIPLGSILVEVVKNGAGALSLEFLTQPPGAIGSGRGGIGPAIQGTLIV
                     IGLASLIGAPVGVLAGIYLSEFAGSSKFPYAVRFLNDVLTGLPSIVVGIVGFIVIVLT
                     IGSFSVWAGAFALSIIMVPIVVRVTEETLKIVPNSIREAGYSLGIPKWKVVMFIVLTS
                     AKSGVLTGIVLALSRIAGETAPLIMTILGTSLFFTGFTDPVDALPLRIWRLASQPYES
                     AHEFGWGAALILILLVLVMNVGLRMLAYKKGFGIKAISA"
     gene            6774..7601
                     /gene="pstB"
                     /locus_tag="VE308_00805"
     CDS             6774..7601
                     /gene="pstB"
                     /locus_tag="VE308_00805"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZA99538.1"
                     /translation="MTHEAIDSGIENTDVLPPTADLQHRYKVSIRNLNAWFGMKQALK
                     NISLDIKKNTATAIIGPSGCGKTTLIRCLNRMHEMTPGATAKGSVILDNIDIYDKRVD
                     PVVIKRRIGMVFQKPNPFPTMSIYDNVAAGLKLNGIKDKRLIGDIVQESLEGAVLWEE
                     VKNELGKPGLSLSGGQQQRLCIARALAMHPEVILMDEPTSALDPIASSKIEELIHNLK
                     KDLTIIIVTHNMQQAARVSDLTAFMYLGELVEYGATKQIFENPQKELTERYISGKFG"
     gene            7677..8276
                     /locus_tag="VE308_00810"
     CDS             7677..8276
                     /locus_tag="VE308_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HZA99539.1"
                     /translation="MAKLSEKSVTTAIESYEKGTSTKKVIFEWSEQLRVLQDEVGDLA
                     IELIARYQPVATDLRFIRSCMEIAYGFSRFGRYAYDIVDVLETMGSIANCDKSAVLEM
                     SCTVREMIHTSVQALQSKDKNAAEMLYRMDDTVDALYRKYLREAITPYEKADKRSFDP
                     RCYISALLILRYLERISDHACYIGDSVHYIVTGTSSPRR"
     gene            complement(9288..10442)
                     /locus_tag="VE308_00815"
     CDS             complement(9288..10442)
                     /locus_tag="VE308_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HZA99540.1"
                     /translation="MKSVVQAPDGHILACTNKSERECFDRMLFATNHVYGENVLKIKR
                     GDLLFLLNLDTDTLYGTFLARSDGAKDIMPEAWEGRYPYQVSVSCNGEVHSLAGAKKI
                     LSSNGITCHSMLKGEMAQSLSQYLKNPNGSLKISKNNADDNNPRLETTTLWDYPRQNY
                     GKSQKGSNKFAGVTPAFVIYNMIKRYTDQGDLVLDPMAGSGTTIDVCREEGRRCIAYD
                     ISPTRPDITQNDARKIPLTDASAEMIFIDSPYGDNINYNDHPGNIGHISAETDEFYKE
                     LDKVMAECYRVLKPGKVLGWLIGDQWVKKKFTPVGFRVYECLCKYFDTVDIICVARRS
                     QTSNSAIWHSRALRFNFYLRGFKYLFIMRKSSIGELPAKRKVKWMHYSRI"
     gene            11128..>11584
                     /locus_tag="VE308_00820"
     CDS             11128..>11584
                     /locus_tag="VE308_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZA99541.1"
                     /translation="MTPITVAKFGGSALGIEGAMIPQIVDRIKQLQQESKVVTVFSAP
                     LIEYNGKIISMTDMAIYVGRSYASSTPVEIEVLREIYERISSKYLDSHRRHEFLDTLD
                     KFYRQVIISLKQAAENRRFVDVIRSRTLAYSGEVTMSYLMDYVMQNIGLK"
BASE COUNT         3224 a         2483 c         2654 g         3223 t
ORIGIN      
        1 ggtcctggct cgccaaagta tcttaccgat gcggctaaag aagccattgc caagtcacat
       61 tacatcatag gctacaagta tccactgtcg actattgaag gtgttattga caaaagcagg
      121 cagcaggtgt ttcaagtcac aatgcagaca caagaaggcg tctatcagga tatttatacg
      181 aaaatgaagg atggcgagta ctgcactgta ccttttacag gtgatgccaa tttctccgaa
      241 tcggaggttg tcgacaggtt gctggaaata tttgggagcg acaatgttga ggtgatcccc
      301 ggtatcagct ccatccaagt ggctgccgcc aaggcccatg tctcgactga caaggctttc
      361 attgttacct tccatgtgac agatgacata gagcagaaaa agcaggaaat gctgcaggca
      421 gttaggcagg gccggagcgt aatactgcta cctcggcctt ggccaagaga cagatcaagg
      481 cactttatgc attctgaaat tgcaaaattc ctacgtaaaa gtggcatcga cactactaag
      541 ctgaaggttt gggtctttga gcatctgacg actgaaaagg aaacaacatt cagaggcacg
      601 gctgtcgacc ttgaaggaaa ggagttcagt gatctgtctg ctatggtagt agatcagaca
      661 aagaggcaaa catacctcaa tttctagccg gagtttaaat attaaattgg gcttattttt
      721 tgtgtatgtc ctcaatacaa aataaatctg ttggcattat gcggaccgat gataagggtt
      781 caatcagata ttgtgttgaa tgtggtgcaa agatgcctaa gtcacagaag atctgtcccg
      841 catgtggcga aagccaataa caccttcttt atgtcctctt tttttgttat aaaatcttaa
      901 aaccttggtt tagattattt tagggaagca tcctctgccg cagagagata ttcttcatgc
      961 ttgacgggca tatgtcattt atcgcaacaa ccatgtgatg gtggcacttg tagtagtgat
     1021 ccaaagcctg taaggctaga tgtgacttca ccggtactca agaagaagta tcgttggagg
     1081 atcgatctat gcaggagtag taggtcaata ggtggaattg taacaaaata ttcgctcagc
     1141 cgataagcct tgtctaaaag tattgcataa tgaggcacag caggtctgcc tagaagaacc
     1201 ttcatcattc caccggatct acggcaatgc tcgcgtcaag accgcccagt cgttctatta
     1261 tctgccactc tattactgcg gctacaaata atatccctgt ggcaatgcca agttctatca
     1321 gcgtcgggat gatgtattta cgccaaggta ttctcttaag aagatagtaa attagcatcg
     1381 cgctccttga catggcaagg ccatacacga atacttccat gatgccaaag ggcgttatca
     1441 ggatggcaag cggcgaaacc tctcctagca taggtgactg acttgcaaga gcgctgaaaa
     1501 ttgagccggt tgcaaaacca gagaatgctc caaatgctga gcctactgca ggtacaaaca
     1561 ttatcagggt gattcggacg ttgttcaaaa agatgccgtt ctggtcaatc ccctctatct
     1621 gtttcgagaa ctcacgcatt aatgtctcgg cttcctcctc actcataggt actgcccctc
     1681 caatggaata ggcaatcaga aatgcagctg caccaaacgc gaggtagatg aggcgccact
     1741 tgttgatctt aaaacccaac aggaatggtt gaaagtggta ttgtataatt agttttatca
     1801 tcctcccgcc ccttctgccc aacttcgtct ttttatgtat gttgaacata acaatagcgg
     1861 tgaaccattc cgcaacaaca acaagtgcag gaaatactgt ttttccgcag ttctttgcaa
     1921 ttggtaatga tcctatagac tttgtggtcc aggacattgc ggtaatcatg gtagtagcgg
     1981 cgataatgct tgcgatcaca tacaagctga agcagcctat ggtcataggc tatattattg
     2041 ccggtatgat aattgggccc tatactccac ctttcaacct tgtccggagt cccgagactc
     2101 tgaatctttt tgcagaacta ggcataatca tgctactctt tgttactggc actgaattcc
     2161 ctatagccaa actccgctcg gtgggcaaga tatccgtagc tactgctctt gcagaagtgc
     2221 taggaacgct cttgatagtg ttttttatcg cccaaagcct tgggttccaa ttttatgatt
     2281 ccctctttct tgccttggca ttatcgatta ctagcaccgt catcacgatc aggatattgg
     2341 aggatgtcgg tctaatacgc gataaatcgt ctacgctcat tcttgggatg ttgatagttg
     2401 aggatataat tgccatcagc gctcttggca tcttgcaatc ctttgttgca actggcggta
     2461 acatgtcaat tttgaacatc gccatcgttc ttggaatagt gggcggcttt atcggtagtg
     2521 taatcatagt aggcagcaag ttcgtccccc ggataataga tcgggccggc aaaacaaatg
     2581 attatgcgct gctcctgata acaatcctca gcatcgcctt tgggctgtca ttccttgcaa
     2641 atggccttgg catgtctgtc gtggttggtg cctttcttgc cggcgtactt gtcgccgaga
     2701 gccggagtgc agcagtggca aggataatca caatccctct gcgggatatg tttgcagcgc
     2761 tatttttcat atcaataggg gcgctcatga atgtgtcgct gatccctgca ttcataatcc
     2821 ctgcaataat tctcatactg acctcctttg cctcaaagct cctgatagtc actggcattt
     2881 tggtaagggc gggctatgac agcacaactg cgcttagggc tggatttgga atatcggcta
     2941 ccaagggaga agtgtcacta attgttgcca agggcggaca ggatgtaaac gccatcactc
     3001 ctttcattct gccactgctt ggtgtagtca caatagtgac gacgtttata ggaccattta
     3061 tcataaaggt cggaagtcga ttcaagctga ctgagtcaac tgaagcagaa gatgcaaaga
     3121 aagaaaaaga agaagaagaa agttagttgt aaaaaatttt tccagcagat cggcgcaact
     3181 gcaattgatc ctaagagatc gcagactagc acaagtgatc gaagtagtag atgtgcaagg
     3241 tgcaatggtg atgccagaat cgaaataatg acaattttac tggttctggc ttctgccacc
     3301 gtgggtgtgt ttatctctac atgaccagag gtaggtgaat ttgggcgaag actttggttc
     3361 ttcctgagcc cctactctga cctccatagt ttttccttaa tattactata tagaatacgt
     3421 agatcatatg gactagccat aaaagcctct tgttcttgcg aactactata gtccatgtct
     3481 gcaagacgtg tcaaaattct gaattttact atccttgcag gcttgctgac attattattg
     3541 tctgcgtttt ttaccggcat tttcatgcca cttggaaatt ctaatgctga gcagagaatt
     3601 gatcttaatg gagctggcgc aaccttccca tttccattga ttgatgcttg gcgtgttgaa
     3661 taccaaaaaa tcaagccaga aataaacatc aattaccagt cgattggcag tggcggagga
     3721 gtcaaacagt tttctgaaaa gattgtggac tttggcgcta ctgatgcgcc ccttacctct
     3781 tcagagatgg aaaaagcccg cgaagcagta cacatacctg aaacaattgg atccataatt
     3841 gtctcctaca acatccctga ggttccagat aagggattaa agcttacagg accaattcta
     3901 gcaggtattt atctgggaaa aattacaaaa tggaatgatc cgcagataca gaagctgaat
     3961 cccgaccttt tcctgcctgc gcaaaacatc gttgtagctc ataggtctga tggctccggc
     4021 acgacatttg tatggacaga ttatctctca aaggtgagca atgaatggaa ggctcaggtg
     4081 ggcaagggca aatcagtcca gtggcccaca ggcatcggag ctcctggcaa tgaaggtgtt
     4141 gcaagcacta tcaagagcac gccttatacg atcggctata ttgagctgtc atatgcgcta
     4201 actacaagaa tgccatatgc ctacttggaa aatcaagaag gtaattttgt agaaccatct
     4261 attgattctg tgcgcgctgc agtagcttct acagccaagc tcctgcccag aggagacgaa
     4321 tcatgggagc agatatcaac aactgatgcc cccggcaagg attcctatcc aatctctagc
     4381 ttctcttata ttctcttata taaagaactg agcaccaacc ctaggattga tagcatggac
     4441 aaagccagag ctcttgtaga ctttgtctct tgggtcataa ctgacggtca gcaatttgct
     4501 gatgatttgg gatatatagc attaccagat gatgttgtaa tactaaatca ggatacactc
     4561 agctcactta cttacaaggg ccagcctgtc cttaccacag aaagtgaaac tgatcccgca
     4621 tctccagaaa ttctggtacc atttgtagcg gctgctgccg taatactgcc aattgtggct
     4681 gctgtaggga taatttatgt gcgaaggaaa aaccgcggat ctttgtcaaa agaagtaact
     4741 aaaataggag caaaaaaaga gtcagtccat gatcggcttt cgcctttttt actcaagagt
     4801 acgcctacag gagacaaaat atttcgcttc atagtgatag gcgcagccag ctatactcta
     4861 gtgctgctcg gtcttgtagc catttccata ttttctgggt cggctgaagt gttttatagc
     4921 gaaggagtaa taggattcat aacagggaca gactggaatg cagttgaagg acgtgagtcc
     4981 tatggcgctc ttccttacgt cgttggcacg ctgataagct cgggtattgc aatgacaata
     5041 ggggttccaa taagcttagg aatagccatt ttcattgtag aaatagctcc gtataggatg
     5101 tccattccat tatccttcgt aatagaactg ttggctgcgg tgcctagcat aatctatggt
     5161 ctttgggcgc tctttgtatt tagattctgg atacgagact ttattgaagt gccattaaac
     5221 catgcgtttg gcgaaacaat cccaattttt gcagggaccc cttttggcct agatgtcttt
     5281 actgctggca ttgtactggc aataatgata atccccaccg tatcttcaat ttcacgcgag
     5341 gtaataaggg cagtgcctaa tatccaaaga gaggcagcgt acagtcttgg agcaactagg
     5401 ctggaaatga ttaagaccgc tatatttcca tatgccaagt ccggactatt tggagcagcc
     5461 atacttgggc ttggcagggc ggtaggtgaa acgatacttg tgactatgat aattggaaac
     5521 gctattggag catctgccat ccccaattcg ctattttcgc caagccagac ccttgccagc
     5581 ctaattgcca acgagtttaa cgaagcggta acatcacttc atgtttctgc actaattggc
     5641 cttggcgtag ttctgttcct gcttacaatt ggcatcaata ttggagcgcg actcctggtt
     5701 tctcagatcg caaaagtttc accaggaagg gagtgattga cataatatgg cagaacaaaa
     5761 tgaaagatta actgaaacat ctgcaaacga tggcggtagc agaagacgcc ggatcgagat
     5821 ccagcaaatg atggataagc agtcaattag gcgccgactg tttgacaaga ttgccacagg
     5881 cgttgtaatg gcatgtgtca tcgccgccat catcccgctt ggcagcatat tggtcgaagt
     5941 tgtcaagaat ggggcaggcg cgttgagcct agaattcttg acgcaacctc ctggagcaat
     6001 aggttctgga agaggtggga ttggtcccgc cattcagggt acgcttatcg taattggtct
     6061 agcatcgctt ataggtgccc ctgttggagt actggccggc atatatctgt cagaatttgc
     6121 gggcagcagc aagttcccct atgctgttag attcttgaat gatgtcctta caggccttcc
     6181 gtccattgtg gttggaattg ttggctttat agtcattgta ctgacaattg gttcgttttc
     6241 agtctgggca ggcgcttttg ctctctctat aattatggtc cctatcgttg tgcgggtcac
     6301 agaggagacg cttaagatag ttccaaattc tatccgtgag gccggctatt cattaggaat
     6361 tcctaagtgg aaggtggtca tgtttattgt actgacttcg gccaaaagtg gggtgctaac
     6421 aggaatcgtc cttgcccttt caagaatagc cggcgaaact gcgccgctta tcatgacgat
     6481 acttggaacc agtctcttct ttaccggttt taccgatcct gtggatgctc tgccgcttag
     6541 gatatggcgc cttgcctcac agccttatga gtcagcccac gagtttggat ggggagctgc
     6601 gctgatactc atactcttag ttcttgttat gaatgtaggg ctaaggatgc tggcatacaa
     6661 aaaaggattc ggaataaagg caatttctgc ctgaatgtgg cgacactaac tgttgttttt
     6721 ctatatagag aatatagata atacataatt agccatgggg cgaagcctaa ctagtgaccc
     6781 atgaagcgat agatagtggc atcgaaaata ccgatgtcct gccacccaca gctgatctgc
     6841 aacatcgtta caaggtttct attagaaatc tcaatgcatg gtttggaatg aaacaggctc
     6901 tgaaaaatat tagccttgac ataaagaaaa acacagctac tgcaatcatt gggccgtctg
     6961 gctgcggcaa gactactcta atccgatgtc taaaccgcat gcatgaaatg acgccgggtg
     7021 caactgcaaa ggggagtgtc attcttgaca acattgatat ttatgacaaa agggtggatc
     7081 ctgttgttat caaaaggcgt attggaatgg tcttccagaa gcctaaccct ttccctacaa
     7141 tgagcatcta tgacaatgta gcggctggac tgaagctcaa tggcatcaag gacaagaggc
     7201 ttattggaga cattgttcag gagagtcttg aaggcgcagt tctctgggaa gaagttaaga
     7261 acgagcttgg caagcctggt ctgagccttt ctggcggtca gcagcagaga ttgtgcatag
     7321 caagagcact tgctatgcat cctgaagtga tcctgatgga cgagccgaca tctgcccttg
     7381 acccaattgc ctcttctaag atcgaggagc tgatacacaa cctcaagaaa gatctgacca
     7441 taataattgt tacacacaat atgcagcagg ctgcacgcgt gtctgatctc accgctttta
     7501 tgtatcttgg cgagcttgtt gagtatgggg cgacgaagca gatatttgag aatcctcaga
     7561 aggaacttac tgaaaggtac atatctggca agttcggcta agcaagatag acatttggta
     7621 atttgacccg tctattagac cttggaatag accgtatacg gaatatcatc atggatatgg
     7681 ccaagctttc agagaaatct gtgacaactg ccatcgaatc atatgaaaaa gggacaagta
     7741 caaaaaaggt catctttgaa tggtcagagc agcttagagt actacaggat gaagttggcg
     7801 accttgcaat tgagctgatt gcccgctatc agccagttgc caccgacctg cgcttcatcc
     7861 gatcatgcat ggaaattgcg tatggatttt cccggtttgg ccgctatgcc tacgatattg
     7921 tcgatgtgct tgaaaccatg ggttcaatag ccaattgcga caagagtgca gttctagaaa
     7981 tgtcttgcac agtcagggaa atgatacaca caagtgttca ggcgctgcag tcaaaagaca
     8041 aaaacgcagc agaaatgttg tatcgaatgg atgatacggt tgatgcactt tacagaaagt
     8101 atttgagaga agccattaca ccatacgaaa aagctgataa aagatctttt gatccacgct
     8161 gctacatctc tgctctgcta atacttcggt accttgagcg catttcagat cacgcctgtt
     8221 atataggtga ttctgtgcat tatatcgtta caggcacatc aagcccaagg cgctaaacgt
     8281 gtatgtatgt tgagaattcg aggcaatata tagtaataat gtagtctatt ctcccaatat
     8341 acttatttgc agcttcattg gtccacaact gtgaggatag gatcagatca tgggaagttc
     8401 gaaaggtgca tcgaaggtgc aatttacatg caggtcgaca tacatcctat cgctaccaaa
     8461 aagatggatt gaagaccttg gattgtttaa gttaccctgt agttgccaag agcatagagc
     8521 gagttgccga tcatgcctac agtataacca acaaagcaat taaactaaaa gaagacaaga
     8581 taccaaagga atctctttga aagatagaga aaaatgagca gcttgcattg atggtgctaa
     8641 gagactgttt cgaaacatta ctgcggcgcg actttcaact tgcagacagc tcgttctagg
     8701 aggtattgga agtacaaccg agtatgtaaa cgacattgcg gaggctgcca tgaacgggac
     8761 gatagatgaa gtgacagaaa agcacagcgc caaccacaga agacaataat aatatctgac
     8821 aggccttaaa gggtccatat atgcatagct ggatgcggtg gcttgtagtt ggcgtcagcc
     8881 ttggagcagt ggctgtaata gccaccattc tcgcaataat tgcaggccaa taattttaga
     8941 tcgatgacat gatgtgcttg atgctgatca actacttatt ctaaatttac ttagtctgcg
     9001 acgcgcattg taggccgcca agcaagcata gttctaggcc ttgatgcaaa tattttctgc
     9061 taatcctgtg tgttaagtct gagcaactgt agcagctaga attaggattt gagtaagtat
     9121 gctatgggtc tgcattgctt cacagaaacg attatgtgta cttacacaca cccactcact
     9181 gctcacatga gctgcataca gtttgtcaaa acatgcagtg caattcctct tctgctgtct
     9241 tcaacaagag atgcacagat tatgttgacg catacggcag aaagaatcta gattctggag
     9301 taatgcatcc atttgacctt tctttttgca ggcaactctc caatcgacga cttcctcatt
     9361 ataaagagat acttaaagcc gcgaagataa aagttgaagc ggagcgcgcg gctgtgccat
     9421 attgccgagt tagatgtttg actcctcctt gctacacata taatgtccac tgtgtcaaaa
     9481 tatttgcaaa gacactcgta tactcgaaag ccaacgggtg tgaatttttt ctttacccac
     9541 tgatcgccta ttagccagcc cagcacctta ccgggtttga gcacacgata gcactcggcc
     9601 atcaccttat caagctcttt atagaactcg tcagtctctg cagaaatatg gccgatgtta
     9661 ccaggatggt cattgtagtt aatgttgtcg ccatacggtg aatcaatgaa tatcatctcg
     9721 gcacttgcat ctgtaagcgg tatcttgcga gcatcattct gagttatatc aggtctagta
     9781 ggcgaaatgt catatgctat acatctgcgc ccctcctccc tacatacgtc gatggtagtg
     9841 ccactccctg ccattgggtc aagcacaagg tcgccctggt ctgtgtatcg cttgatcatg
     9901 ttgtatatca caaacgctgg tgtcacacca gcgaatttgt tgcttccctt ttgagacttg
     9961 ccgtaattct gccgcggata gtcccatagt gtagtagttt caagccgtgg attattgtca
    10021 tctgcattat ttttggagat cttcaaactg ccattggggt tcttgaggta ctgagaaagc
    10081 gattgcgcca tttcaccctt aagcatgctg tggcaggtga tcccattgct agacaggatc
    10141 ttttttgcac ctgcaagaga gtgtacctcg ccattgcaag atacactcac ctggtatggg
    10201 tatctgcctt cccaggcttc aggcatgatg tcctttgcgc catccgacct tgctaggaaa
    10261 gtgccgtaga gagtatctgt gtccaggttg agtagaaaaa gaaggtcacc tctttttatt
    10321 ttcaaaacat tttcaccata aacatgattt gttgcaaaga gcatcctgtc aaagcattct
    10381 cgctcactct tgttggtgca ggctaagatg tgcccgtctg gcgcctgcac tacactcttc
    10441 attttatcaa tggagatatc ttggcaagta gatttaacga tatcggaggt gacaataatt
    10501 tcataaaaga tatgaaagaa gagcgtgttc tgctttagca gttatttttt tgcgtttttc
    10561 atcttaagct tttattcatg caaaccgatt attttcacac tgaaaatttc agttgcactt
    10621 gcaatgtcga ggtcaaattg agttctaccc tatttggaaa tagaaacgtc aaccatttta
    10681 gtattatata taatattcaa aaatattgca tgattattgg aaaaaaacgt caatttgtgg
    10741 agagcaaatt atattttaaa aaccttttac gacaccaatg gcactattat tgtatcatga
    10801 cactctgcta tctttgcagc acaagggcag cctcaatgga atgctctgaa tgcaagagac
    10861 ctgtatgcaa aaactgcaca aaagatttcc cattctcaag cagtgcatgc cttgaatgcg
    10921 cggaaactat aaagacgggc ttgggaggca tcagaaaaga cgaagagatt ttttaacttt
    10981 tttcttattt tttctaaggt tatagttata tcaaaaattg aagtatcttc tttgatgctc
    11041 gccattaata acttaagata cctttctcct atttcctatc tttaatatag ggaattactt
    11101 gcaactagcc cagagatctc ctaacaaatg actcctataa cagttgcaaa gtttggcggc
    11161 agcgcccttg ggatagaggg agccatgatc ccccagattg ttgacaggat aaaacagctg
    11221 cagcaagaat ctaaggtagt cactgttttt tcggcacctc taattgaata taatggcaaa
    11281 atcatctcta tgactgatat ggcgatttat gtaggcagaa gctatgcttc ttccactccc
    11341 gtagagattg aagtgctaag agagatctac gagagaatat cctccaagta cttggacagt
    11401 catcgccgtc atgaatttct tgatactctt gataagttct acagacaggt gataatttcg
    11461 ctcaagcagg cggcggagaa taggcgcttt gttgatgtga taagatcaag aacgcttgca
    11521 tacagtggcg aagtgacaat gtcatacttg atggattatg ttatgcaaaa tataggcctt
    11581 aagt
//
LOCUS       DASQTF010000034         7820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_98951,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000034 DASQTF010000000
VERSION     DASQTF010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98951"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2379)
                     /locus_tag="VE308_00825"
     CDS             complement(<1..2379)
                     /locus_tag="VE308_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HZA99542.1"
                     /translation="MHIKKLEVYGFKSFGFKNTAVHFERGLVAITGPNGSGKSNILDA
                     IMFAIGENSPKTLRVDKFQSLFHDSQNSNHRLIRVSLTFDNRDRGIPIDSDTVTLTRG
                     IEGQNGESQYWLDDKKVTKTAIMELLEVVLAAPNKLNIVQQGMITRISELNSEDRRSI
                     IEDIVGLSYFDEKKAEALKQLDEADRRLEVAFARMGEIRKRIDELETERNEQLRYDQL
                     SMELKRFKAVQISNTLRNLRYKLATSTQILESNNQKSSRLSHLMEETRLEIEKFDAEK
                     SRFIQQVDSANRAKAQVAMRISGIVHDSERTKAMLSEFERRIADIERRLPSIHKSKEA
                     AVQRIESAHSDADRLKKETEQKKAAVNDLKIKLAAIDSEIETINANVAKYSVFRDTTE
                     KRLARMAPLKGRIELAGARVEEKIKTINYKKDSNHSAINLLQSEILTTKSRIAQLERS
                     LESDGVKLHHVVTLASELAESKLNLEKELAGSTNLLVRAENLVNKFEERATIAKNAMN
                     EDLAIAELMKEKDRFSICGLVHDLIRWDKSYERPVLAAGSEWMKAFVVNDIKSMIMIA
                     AYAKEQKLPRLRIIPLDVVSRFKEKQDTPRDEVNVIARLSDFVHSDYDKLPAFLFSDT
                     LLVRNSSTAFMLSRKGYRTVSADGELFEPAGRSMSVDFGGRITDMTKAILFGESVGNL
                     RTMIAELSRLIKEKNIEVQGIFKRISAGESERVQLELLIRDVERSITKEKESLEAKEK
                     SISGLILDNEVLQSEADLLSTELEKYSRRLTLLSSAMEKLSSRLQSVREEPSS"
     gene            complement(2999..4078)
                     /locus_tag="VE308_00830"
     CDS             complement(2999..4078)
                     /locus_tag="VE308_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZA99543.1"
                     /translation="MSKKIKVALAGIGNCASALIQGTKYYNTGSNGESNDSIGLTAFD
                     LGGYEPRDIEFVAAFDVVDSKVGKDLSEAIFAEPNNTVKIANVDKSEVIVEKGEVLDG
                     LGNHYREKVHISDKPPANVAKVLKDSGAEILLNYLPVGSRQGTRYYAEACLDAGCAFI
                     NAIPVFIASNSKWQQAFEDRKLPCAGDDVMSQLGATVVHKTLVKLFVDRGVTVDQTYQ
                     LNIGGDMDFYNMLDQDRLEDKRVSKTSAVAAMAPYEIPMRIGPSDFVQFLDNEKVCYI
                     TMKGRYFGNIPVELDLKLKVVDAYNSAGVMIDAVRATKIALDRGISGSLDSVSAYCFK
                     HPPVQLPYSVAKSNFLEFVEGKKER"
     gene            4232..5902
                     /locus_tag="VE308_00835"
     CDS             4232..5902
                     /locus_tag="VE308_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZA99544.1"
                     /translation="MANPIIVLLGSFYWVLILDALIWLSTVLILGLSIYSIRSLIFIC
                     SVTRARANNNLHNIPKIGAIIPSTAIRAVSLHEQGVIADSCGDIGGVNRTSGQRIDEE
                     YPFISIFVATYNESPVIEKLLKSFAALSYPADRFEIIIVDDSTDDTYQKIQTRLSDLR
                     NLKVIHRANRAGWKGGALNVALDAMDKRASNVLVLDADNILLVDTVERFVSRFIEEQY
                     SYKEKRVSVLAIQGFPISKNNPEADNESGIKVKLGNWIARAIDFRLSQRNLIEFTAKD
                     LLNLPVQITGSLFMIRADVIKSIKFSNDLCEDWDLTVDLYCSQPSPSLSSTPDVMTSD
                     SNTEPICNYASNNIFPKPVAVLLKPKIIFVRELVSYCEATTDLPTYFRQRMRVSEGHT
                     RGFRRKIRLITKNNMLSLVDKVELFLNGLQYAKFIFVLGIGIINMILILMLLSGSYNN
                     SQQFLMNLFGISFSLQAANLAIAIARITWAAKICRPVRRYDIEDVLSLMALYVITTPA
                     FVIGSLRGFFQDKGTFYKTRRNLPKESKLSKPSVLSESTKSSPSYTGS"
     gene            complement(6237..6827)
                     /gene="cbiT"
                     /locus_tag="VE308_00840"
     CDS             complement(6237..6827)
                     /gene="cbiT"
                     /locus_tag="VE308_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HZA99545.1"
                     /translation="MVVWDYRTPGIPDELFERTDEVPITKEDIRAIVISKLRLKEGHS
                     AIDVGCGSGSITVELCLQTKGKVYAIDFDEKAVELTKKNLQKFGAKAEEVILGKAQDI
                     LPKLLEVDAIIVGGTWGDTKRVIELAAGSLKKGGRIVIDTIVIETLYQALTTINDLEL
                     KEIDITQVTISKARKVTTGTMMLARNPVMILSATKV"
     gene            complement(6857..7156)
                     /locus_tag="VE308_00845"
     CDS             complement(6857..7156)
                     /locus_tag="VE308_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99546.1"
                     /translation="MDIFERLYYMRALVGVTAGIVAGIVIPVEFDQYTSIVTAILVGI
                     VFYFASLAIGRRIAKNVPRDKRKKVATEGIMPFIFLLLTFMIIVYTALHQSSIVS"
     gene            7308..>7820
                     /locus_tag="VE308_00850"
     CDS             7308..>7820
                     /locus_tag="VE308_00850"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HZA99547.1"
                     /translation="MPLLSFVMELSSIELRYLINDIRSRVISSSSGYYVSSINAITKS
                     SLFFRLHHPVQEDIMFVLSTRGIWITRLKFKPVEENNSLESIAQKELERTKLELIEQV
                     GSERIISLRFRHPDGKVRIVVGEFFGDGNLIICNENMQIIAILNPIHVRHRTLSVGLR
                     YVYPPARGVDV"
BASE COUNT         2116 a         1728 c         1574 g         2402 t
ORIGIN      
        1 acttgacggt tcttccctta cgctctgtag ccttgacgat agcttttcca tggcagaaga
       61 aagaagggta agcctccttg aatatttctc aagctctgtc gaaagcaagt cggcttctga
      121 ctggagaacc tcattatcta agatgagccc tgagatggac ttttcttttg cttccaaaga
      181 ttctttttct ttagtaatgc ttctctcgac atctctgata agaagctcta gttggactct
      241 ctcggattct ccagcgctta tgcgcttgaa aattccctgt acctctatgt tcttctcctt
      301 aatcagcctg gagagctccg ctatcattgt acggagattt cccacggact cgccaaaaag
      361 tatcgctttt gtcatatcag ttatccttcc tccaaagtcc accgacattg aacgtccagc
      421 cggttcgaac agctcaccat cagctgacac agtcctgtag cccttccttg atagcatgaa
      481 tgctgtagat gaatttctta caagcagtgt gtctgagaac aggaatgctg gcaacttgtc
      541 ataatcagaa tgtacaaaat ctgacagcct cgcgattaca ttgacctcat cacggggtgt
      601 atcctgcttc tccttaaagc ggctgaccac gtcaagtggt attatcctaa gacgtggaag
      661 cttttgctcc ttggcgtagg cggctatcat tatcatagac ttgatgtcat tcactacaaa
      721 ggctttcatc cattcagagc ctgctgcaag cacaggccgc tcgtaggatt tgtcccatct
      781 tattaagtcg tgcacaagtc cgcagatgct gaatctgtct ttttctttca tcaattctgc
      841 aatagcaaga tcctcattca tggcgttttt ggctatggtg gcccgttctt caaacttgtt
      901 cacaagattc tctgcacgca ccagtaggtt tgtagagccg gccagctcct tttcaagatt
      961 taatttcgac tctgcaagtt cggatgcaag tgtgacaaca tgatgcagtt taaccccatc
     1021 agactcaagc gacctttcaa gttgtgctat gcggcttttt gtggtcaaaa tttctgattg
     1081 gagcaaattg atggctgaat gattagagtc cttcttgtaa ttgatggtct tgattttttc
     1141 ttcgactctg gccccagcaa gctcgattcg accctttaac ggtgccatcc gtgcaagcct
     1201 cttttccgtt gtatctctaa aaacagagta tttagcaaca ttggcattta tcgtttcaat
     1261 ttcactatcg attgcagcaa gcttgatctt aaggtcattg acggcagcct tcttttgctc
     1321 tgtttctttc ttcagtctat cagcgtcaga atgggcgctt tctattcgct gaactgcagc
     1381 ttccttgctc ttgtgaatgg aaggcagcct cctttcaatg tcagctatcc ggcgctcaaa
     1441 ctcactcaac atagcctttg tccgttccga gtcatggaca atccctgata ttctcatcgc
     1501 aacctgcgcc ttagctcggt tagcagagtc aacctgttga ataaatctcg acttttcagc
     1561 atcgaacttt tcaatctcta gccgcgtctc ctccattaga tgtgaaaggc gtgatgactt
     1621 ttggttgttt gattcaagga tctgagtgct tgtagccagc ttgtacctca ggtttcgtaa
     1681 ggtgtttgat atctgcactg ctttgaacct ctttagctcc atgctaagct ggtcatatct
     1741 tagctgttca ttcctttctg tctcaagctc atcgatacgc tttcggattt cacccatcct
     1801 tgcaaatgca acctcaagcc tcctatcggc ctcgtccagc tgcttaagag cctctgcttt
     1861 cttttcgtca aagtatgaga ggccgactat atcctctatt atacttctgc gatcttcaga
     1921 atttagttca gaaattcttg tgatcatacc ctgctgtaca atgttaagtt tgttaggagc
     1981 tgccagcaca acttcaagaa gctccattat ggcagtcttg gttaccttct tgtcgtcaag
     2041 ccaatactgg ctctcaccat tctgaccctc aattccacgc gtcaatgtga ccgtgtcaga
     2101 gtcaataggt atacctctgt ccctgttgtc aaacgtcaag ctaacgcgga tgaggcggtg
     2161 gttgctgttc tggctgtcgt ggaacaacga ttggaacttg tccaccctca gagttttggg
     2221 actgttctcg cctattgcaa acatgattgc gtcaagaata ttagacttgc ctgagccgtt
     2281 tggaccagtt atagccacta atcccctctc aaaatgcact gcagtgttct taaaaccaaa
     2341 tgacttgaag ccgtagactt cgagcttttt aatgtgcacc aaggatagct atctgattag
     2401 ttttcaggta agctaattat acgttatcgc gctcgatcca tctcctttta gtaaaatatt
     2461 tttcgcatgt atatagtcgg aaaagctttc tcgttagagt actatataac tacctgttct
     2521 ttcggcagga ggtgttcgtg ttactgcact agcaagcctt gctatcggaa tagcctttat
     2581 aacttaatcc aggatacatt cttgcaaacc tatttgagaa tactcacaaa atttatgatc
     2641 ccaaggatca tggcggatcc taattagttg aacatcaaga acaaacttag tctttttaag
     2701 aaaggattcg ttcaacattt caaccagaag aaatttttta tgatcaaact attaattata
     2761 agggctatgc atgtgaattg tgtcgaggct tacaagtgca ctattttcca gttagaggaa
     2821 ttgcctgccg aaatttagca caaaacgcat tttctatcat atcgctgcga ccaaaaacgt
     2881 atctgatgtt tcagtctcaa tatgccatga gagttatgga acgatatact gtctgaaagt
     2941 tcactcagag aaaaaaagac taataataga tcaaagaaaa tgtgaaaagg gtcaaattct
     3001 atcgttcctt tttgccttcg acaaattcta agaagtttga cttggcgaca gaataaggca
     3061 actgcactgg tggatgcttg aagcagtaag ccgatacact atcaagtgat ccagaaatac
     3121 ctctgtcaag tgcaatcttg gttgccctca ctgcatctat catcacgcca gcgctgttat
     3181 acgcatcaac cacttttagt ttgaggtcga gctcgacagg gatattgcca aaatatctcc
     3241 ctttcatggt tatgtagcac actttctcgt tatccaagaa ttgcacaaaa tctgatggtc
     3301 caatcctcat cggtatttca tacggtgcca ttgcagctac tgcacttgtc ttgctcactc
     3361 tcttgtcctc tagccgatcc tgatccagca tattgtaaaa gtccatgtca cctccgatgt
     3421 ttagctggta ggtctggtct accgttactc cgcggtctac aaagagcttt accagtgttt
     3481 tatgaacgac agtggcacca agctggctca taacatcgtc accagcgcat ggtaatttac
     3541 gatcttcaaa tgcctgctgc catttcgagt ttgacgctat aaatacaggg attgcattaa
     3601 taaaagcgca acctgcatca aggcaggctt cggcatagta tcttgttcct tgtctgcttc
     3661 caacaggtaa gtaattgaga agaatttcag cgccactatc ttttaggacc ttggccacat
     3721 ttgcaggagg tttatcagag atgtgtacct tttcgcggta gtggtttccc aaaccgtcta
     3781 gaacttctcc tttttcaaca attacctcgg acttatcgac gttggctatc ttcacagtat
     3841 tgtttggctc tgcaaaaatt gcctcagaga gatcttttcc tacttttgaa tcaacaacgt
     3901 caaatgctgc cacaaattca atgtctcttg gttcgtatcc tcccaaatca aatgcagtaa
     3961 gtccaattga atcatttgac tcaccgttac tgcctgtgtt ataatatttt gtaccctgta
     4021 tcagtgcaga agcgcagttt ccaatacctg ctagtgcaac cttaattttt ttagacatac
     4081 cctatgaatt aaaaagaaga tcttcgtatt tatcagactc tatagatcaa ctaggaagat
     4141 taccttatat gatcaaactg aacatcacat tagtcgcttt atatccttta tttttagtat
     4201 agaatcgact taaaatcaga agcagaattt tttggcgaac ccgattatag ttctacttgg
     4261 atcattctat tgggtcttga ttcttgatgc tttgatatgg ctgtctacag ttttgattct
     4321 aggcctttca atctactcaa taagatcact tatctttatc tgtagtgtta caagagcaag
     4381 agcaaacaac aatctacata atataccaaa aataggcgcc attataccat cgacagctat
     4441 tagagcggta tcacttcatg aacagggtgt tattgctgac agctgcggag atattggagg
     4501 agttaatagg acctcaggtc agcggatcga tgaggaatat ccatttattt caatatttgt
     4561 cgcaacctac aatgagtctc ctgttatcga aaagctcttg aaatcctttg ctgctcttag
     4621 ttatcctgct gatagattcg aaataataat tgtagatgat tctaccgatg atacatatca
     4681 aaaaatacag actaggttgt ctgatctacg gaatcttaag gtaattcaca gggccaacag
     4741 agcagggtgg aagggagggg cattaaatgt tgcccttgat gcaatggaca agagagcatc
     4801 taatgtattg gtacttgatg cagacaatat tttgttagtg gataccgtgg aaagatttgt
     4861 atcacgattc atagaagagc aatactctta caaggagaag agagtctcag tattagcaat
     4921 acagggcttc cccatttcaa agaacaaccc tgaagctgac aacgaatccg ggattaaggt
     4981 aaagttgggc aactggatag cacgggcgat tgattttagg ctttcccaaa gaaatttgat
     5041 cgaatttact gcaaaagatc tcctaaatct gccagtgcag ataacaggaa gtctttttat
     5101 gataagagca gacgtaatca aatctatcaa attctctaac gatctttgtg aggactggga
     5161 cctgacggta gatctgtact gttcacaacc ctcgccctca ttatcatcaa caccagacgt
     5221 gatgacttcg gacagtaaca ctgagccaat ttgcaattat gcttctaata acatctttcc
     5281 taagccggtt gcggtcttat tgaaaccaaa aataatattt gttcgagaat tagtttcgta
     5341 ctgcgaggca actactgatc tcccaactta ctttaggcaa aggatgaggg ttagtgaggg
     5401 ccacaccaga ggcttcagaa gaaaaattag acttataaca aaaaacaaca tgctttcact
     5461 tgttgacaaa gtggaattat tcttaaatgg tctacagtat gcaaagttca tttttgtttt
     5521 aggtattgga ataatcaaca tgatcttaat tcttatgctc ctatcaggta gttacaacaa
     5581 cagccagcaa tttttgatga atttgtttgg gatatctttt tcgttgcagg cggcgaatct
     5641 ggctatagct atagcacgta ttacatgggc agcgaagatt tgtcgacccg taagaaggta
     5701 tgacatcgaa gatgtattga gtttgatggc gctttatgtg atcacgaccc ctgcatttgt
     5761 aataggctca ctccggggct ttttccaaga taagggaaca ttctacaaaa cacgcagaaa
     5821 tttaccaaag gaatcaaagc ttagcaaacc ttcagttctc tcagaatcta caaagagctc
     5881 tccatcttat acaggttcat agagtcagta tctaaattgt tgctgattgc atgggttgat
     5941 gcagtagctg aaggagcagg attcacaata aatcagcatc atacaaaaga cacaggaaga
     6001 aaaagaataa cgtgatgcat ctaggatata ttgtcttgag ggtagataaa attgtcctga
     6061 tagaagaaat ttgcacaaga cccgtgtata gatatgtact cgtaatagct gcagtgtctt
     6121 gcatgattct ctatgtctaa ttggtttggc tttatcgcta actgatagca taggccatta
     6181 tcggcaacag agctcgatct ttggatttga gaaaaaattt agtagcaagt gtatgatcaa
     6241 actttggttg ctgataatat catcacaggg tttcttgcaa gcatcatcgt gcctgttgtg
     6301 accttgcgcg ctttggatat tgtaacctgg gtgatgtcta tttccttcaa ttctagatcg
     6361 ttaattgttg taagcgcctg atatagggtc tcaataacta tagtgtcgat gacaatcctg
     6421 cctccctttt tcagactgcc tgctgcaagt tcaatcaccc gctttgtgtc gccccatgtg
     6481 ccacccacta ttattgcatc gacctccaat agctttggca agatatcctg agccttgccc
     6541 agtatgactt cttctgcctt agctccaaat ttctgcaggt tctttttcgt cagctcgact
     6601 gctttttcgt caaagtcaat ggcatatact ttgccctttg tctgaaggca taattctact
     6661 gtgatgcttc cgctgccgca gccaacatct attgctgaat ggccctcctt tagacgtagc
     6721 ttgcttatca ctatcgcacg aatgtcctcc tttgttatcg gtacttcgtc tgtcctttcg
     6781 aaaagctcgt cagggatgcc cggagtgcgg tagtcccata ctaccaactt gctgtaacta
     6841 ataaacacag gtgtatttaa cttacgatgg aggactggtg gagcgccgta tagactatta
     6901 tcatgaatgt gagtagcaga aagatgaacg gcatgatgcc ctctgttgca acttttttgc
     6961 gcttatccct tggcacattc ttggctattc gcctgccaat tgcaagcgag gcaaaataga
     7021 atacaattcc gacgagtatg gcagtgacta ttgatgtgta ttggtcgaac tcaactggta
     7081 tgacaatacc ggcgacgatg cctgcggtca cacccaccag agcacgcatg tagtagagcc
     7141 tctcaaatat gtccaatatg gcgttttgtg gttaaattgt agctagtaaa atgccttgcg
     7201 gctgtgttgt tctgatactt attggaaaaa taagtaacat tactgatagt atgagataca
     7261 ctcaaatttt ctctctctac tattttcagt tgtttaatat ttatggcatg ccattactat
     7321 ctttcgtcat ggagctatcc agcatcgagt tacggtacct aatcaatgat attagaagca
     7381 gagtcattag tagctcctca ggatactatg taagcagcat caatgctata acaaagagct
     7441 ccctcttttt caggcttcac catcccgtgc aagaagatat tatgtttgtc ctctcaacaa
     7501 gaggtatttg gattacacgg ctaaagttca aacctgtgga agagaacaac agccttgaga
     7561 gcattgctca gaaagagctt gagcgcacca agctagaatt aatagagcag gtaggaagtg
     7621 agcgcattat ctcattaaga tttaggcatc cagatggaaa ggtaagaatc gtcgtcggcg
     7681 agttctttgg tgacggcaac ctcataattt gcaacgaaaa tatgcagata attgcaatcc
     7741 tcaatccaat acacgtaagg catagaactc tcagtgtcgg cctgcgctac gtctacccgc
     7801 ctgcaagagg cgtcgatgtc
//
LOCUS       DASQTF010000035         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1118990,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000035 DASQTF010000000
VERSION     DASQTF010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118990"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..647
                     /locus_tag="VE308_00855"
     CDS             474..647
                     /locus_tag="VE308_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99548.1"
                     /translation="MTGAGIILYMLHLGNYTLAAITAGVFAPLIGFHFAVSKVQQHSN
                     VEWSPRRTVKLRP"
     gene            complement(644..1105)
                     /gene="pyrI"
                     /locus_tag="VE308_00860"
     CDS             complement(644..1105)
                     /gene="pyrI"
                     /locus_tag="VE308_00860"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HZA99549.1"
                     /translation="MSDTSRLLVRRIKDGTVIDHIEAGKALLVLRALSITGKEGNVIT
                     VALNVPSSKHGKKDIIKVENRFLETSETNKLALIAPRSTINIIRDYKLLEKRKIQMPH
                     SIVGIFKCPNLKCITNSGEEIRSLIDVIDKEKVVLKCRYCARTLSTDELVF"
     gene            complement(1561..>2188)
                     /locus_tag="VE308_00865"
     CDS             complement(1561..>2188)
                     /locus_tag="VE308_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiol-disulfide isomerase"
                     /protein_id="HZA99550.1"
                     /translation="KYLIDPEGYVRYDHIGEGAYAETEKVIQSLLAERTEYIGANVTI
                     DQSISNPESSQSVDFDRINTPELYFGYEYSRAPLGNSEGYKPDQVVKYTIPGDTQIVP
                     NRIYLDGEWKNNADHMELQSEVGRIVLSYSAKAVNIVAGGKGELNILEDDSPLGVSLS
                     GRDISEDGTVKIDGQRLYNVVEHEGYGDHQIAIEVAGRGFQIYTFTFG"
BASE COUNT          596 a          490 c          464 g          638 t
ORIGIN      
        1 aggattgata acactcttta ttgcaatggg ctttggctac aagctatcga aaaagtttgt
       61 caaggttcta tctcgagata gtgagtagga tacatacgtg aaatcctata gacgatctcg
      121 ctatcaatgc aatgaaaatt agaaaaaact agctatatag cagtcatgta gtgaccctca
      181 tgttagcagg agccattatt cttcttcatg taggaggtgt agccacacaa caaatattcg
      241 gctagcttca aaggtttatt agattctagc tgaatcccaa ataattaacc tatcttatcc
      301 acataacatt attaatatga atattaactt gatttcatct gaattttttc atttaagatc
      361 acactataga aacgccaaat atccaatcag caaaaatagc tggaatgcga aatacaactg
      421 tgaatctaaa gtatatcgct ttgcagctct gtacctgtac ctggattgag ataatgacag
      481 gcgctggcat catactctat atgctgcacc ttggtaacta taccttagca gctataactg
      541 cgggggtgtt tgcgccactg atcggctttc attttgcggt cagcaaggta caacaacata
      601 gtaacgtaga atggagtccg aggaggacag ttaagttacg tccctaaaac accagctcgt
      661 cagttgaaag cgtccgggcg cagtatctac acttgagcac cactttttcc ttgtcgatga
      721 catctatcag cgaccttatt tcttcaccgc tgtttgtaat gcattttagg ttaggacact
      781 tgaagatccc aactattgaa tggggcatct ggatcttacg cttttccagc agtttataat
      841 cgcggatgat gttgattgtt gaccgcgggg caatgagtgc aagcttgttg gtttcacttg
      901 tttccagaaa tctattttcg acctttatga tgtccttctt gccatgtttg ctacttggca
      961 cattaagcgc gacagtgatc acgttaccct ccttgcctgt gatgctcagt gctcgcaaaa
     1021 ccagaagtgc cttgcctgct tcaatatgat ctattactgt gccatctttt attctgcgca
     1081 caagaagccg gctcgtatca gacatttgag gtatcaatgc aatactgcta agtataatat
     1141 cagcataccg ctagattagc agacagagag agagcactaa cgatacattg attattccgc
     1201 tgcactaata gctactttgt gggattattc aaacgctgga aaccaaaagg aaaaatgtac
     1261 agtctgagat gcactcagtg cggcgcagga ttcttgtcca gctacaacct tgaagcccct
     1321 atatgcggca agtgcctcca aaaggccaaa tctttagatt ctactgtaga tcacgaaaag
     1381 gcctgtgtta aatgcggcac acaaggcgta atctatggca aagaatactg ccattcctgc
     1441 tactttggat ttcagaagaa gacttgagcc gatgcagaga aggtttgctt gttaccaaaa
     1501 aaaaggcgtc atcatatgaa tgtttgaaat tttaaaagaa atcaaatggg gtggcaagct
     1561 ttagccaaat gtgaaggtgt atatctggaa ccctctgcct gcaacctcaa ttgctatctg
     1621 gtggtcgcca tacccttcgt gctctacaac attatagagc ctttgaccat ctatcttgac
     1681 cgtgccatcc tcagagatat ccctgccgct taaactgacg cctaatgggg agtcgtcctc
     1741 caaaatattg agctcgccct tgccgcctgc aacgatattg actgccttgg cagagtacga
     1801 gagcacaatg cgtccaactt cactctggag ttccatgtgg tcggcattgt ttttccattc
     1861 accgtcaagg tatattctat tgggcacaat ctgggtatcg ccagggatcg tgtacttgac
     1921 tacctgatct ggtttgtacc cttccgagtt tccaagtggc gcccttgagt actcgtaacc
     1981 aaagtatagc tcaggcgtat tgatcctatc gaagtcaaca ctctgcgaac tttcggggtt
     2041 gcttattgat tgatcaattg tgacgtttgc accgatgtat tctgtgcgct ccgcaagcag
     2101 cgattggatg actttttcgg tttcagcgta tgcgccctct ccgatgtgat cgtaacgaac
     2161 gtaaccttca gggtcgatca gatatttc
//
LOCUS       DASQTF010000036         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_841597,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000036 DASQTF010000000
VERSION     DASQTF010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841597"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1157)
                     /locus_tag="VE308_00870"
     CDS             complement(36..1157)
                     /locus_tag="VE308_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZA99551.1"
                     /translation="MRLLEYQGKELFDEFGIRVPRSHLAHTIQEAREGSNKLGYPLVI
                     KSQLTVGGRGKAGAIVKCKSEDELEPKFNELLHKEVKGELPRGILLEEMADIKKELYL
                     SLFLNRSKRCYSLISSAEGGIDIENAGNKVMVDVPIDGLNPQEAEEIAAKLGLTGQTV
                     ISFVDFTTKLSNMVNEKEAELAEINPVAILQDGSLMGLDAKVIIDDNAMFRHPELKKY
                     EHVSELEKQAEISGFSLVELDGNIAIIGNGAGLVMSTLDMVSDAGGNAGAFLDFGGRA
                     TTETIYEALKVISKINRVEVVLVNLFGGIVRTNLVAQAILDAYNNNLMNVPVFARISG
                     ADSEKAREMLQGSKAKLYNTVEEAIQSAVSALNSSRGSK"
     gene            complement(1711..>2770)
                     /locus_tag="VE308_00875"
     CDS             complement(1711..>2770)
                     /locus_tag="VE308_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZA99552.1"
                     /translation="IGSDHGIPSLITMRPAFGIRGSYLPAILNILQLIGWTTFEIMIM
                     ARAADMLTGSILPYYFWAAIFGALVALLVIAGPLNVVRRWIGKFAVWIAYGTSAIIII
                     NLINSADITALISSPGEGLSFFSALDLVIAMPVSWMPLVADYNRFSKKSKSALWGTFI
                     GFTMTNILFYFGGILLGTSDVLAIVVAIQAMFFGFLILLLIVDEADNAFADVYSAAVS
                     TQDIFAKISQKYLIVGFTTLSAILAMVVSIEQYEVFLLLIGAIFVPLFGVVLTDYYIL
                     KRQKYTEQMFYVRQNRLGIGVPAIIAWTLGALLNYILSPLSPIYMPQLPAIGATIPSL
                     AAASLIYLAITRVQRREQ"
BASE COUNT          748 a          635 c          548 g          839 t
ORIGIN      
        1 gtcaaatatg ttgaacgttt tttccactgt tgttgtcatt ttgatcccct gctactgttt
       61 agagccgata ctgcagactg tattgcctcc tccactgtat tatagagctt tgccttactg
      121 ccctggagca tttcccttgc cttctcagaa tcagctccag agatccttgc aaatacaggc
      181 acattcatga ggttgttgtt gtatgcatct agtattgctt gtgctacaag gttggtcctt
      241 acaatgcccc caaagagatt cacgagtact acttcaaccc tgttgatctt gcttatcact
      301 tttagtgcct cgtagatagt ctcagtggta gctcttcccc caaagtccaa gaatgcacct
      361 gcgttacctc cggcatctga gaccatgtca agggtcgaca ttacaagccc tgctccattg
      421 ccaatgatgg ctatgtttcc gtcaagctcg accagagaaa agccactaat ttcagcctgc
      481 ttttcaagct ctgatacatg ctcgtacttt ttaagctctg gatgccggaa cattgcatta
      541 tcatcaatta tcactttcgc gtcaagtccc atgagagaac catcttgtag aatcgcaaca
      601 ggatttatct ctgctagctc tgcttctttt tcatttacca tatttgaaag cttggttgta
      661 aagtcaacaa aagaaatgac agtttggcct gtcagaccca gttttgcggc aatctcttcg
      721 gcctcttgag gatttagacc atcgattggc acatcaacca ttactttgtt ccccgcattt
      781 tcgatatcaa tgccaccttc agcagaagat attagcgagt aacatcgctt gctcctattt
      841 aggaacaacg acaggtacaa ttcttttttg atatcagcca tttcttcaag taggatgcca
      901 cgtggtaatt ctcccttgac ttctttgtgc aagagctcat taaacttggg ttcaagttca
      961 tcttcacttt tacatttgac aatagctcct gccttgcccc ttccacccac tgtaagctgc
     1021 gattttatta caaggggata gccaagtttg ttgctacctt ctcttgcttc ttgtatcgtg
     1081 tgagcaagat gtgaccttgg taccctgatt ccaaattcat caaaaagctc ctttccttgg
     1141 tattctagca atctcatatt ttgagcaaag aatcaattat cgctatttat aaatcaaatt
     1201 actgttagtt attgaaagta tcaacaactg tatatgctat atcatggttc gatgatatac
     1261 ccagagatgt attttttgaa tttcttttca aaacagttaa gatgcagcag aatggaattg
     1321 aacgagataa gtgttgatac tatcgttttc tttagttctc gcaggttata attttctgtg
     1381 aatagcacag agcgcaaaag acttcttatc ttgtaattct aatgtactca aacttaggag
     1441 tgtaagcgac tttttcttga gctttgtcgt tacaagagcg tatacaatga atatgaatca
     1501 cagagcaaag gctggaaagg actggaatat gcggcttgtg agctgttttt gcatttgggc
     1561 ggggtattat tttcaacatt tgagaacaag gtataatgta gttatcaatg ccgctcaacc
     1621 tcttcccatc atcacccttt gtattgttcc tatcttatag tcattatcac tgttaatatt
     1681 tctattctag atttccgtca tgacttcttc tcattgctct cgccgctgca ctcttgttat
     1741 tgcaagataa atgagtgaag cggcagcgag gctcgggatt gtcgcaccaa tagccggcaa
     1801 ttgcggcatg tagatggggg aaagtggaga cagtatgtaa tttagcaggg ccccaagggt
     1861 ccacgcaatt attgcaggta ctccgatgcc tagcctgttt tgacgcacat aaaacatctg
     1921 ctcagtgtat ttttgcctct ttaggatgta atagtctgtt agcacgactc caaaaagagg
     1981 aacaaatatt gctcctatca aaagcaaaaa cacttcgtac tgctcaatag aaaccaccat
     2041 cgccagtatg gcactgagcg tggtgaaccc aacgatcagg tacttttggc tgatctttgc
     2101 aaatatgtcc tgtgttgaga ccgctgcgga atagacatct gcaaatgcgt tgtcggcttc
     2161 atcgactatc aacagtagta tcaaaaagcc aaagaacatg gcttgtatag caaccacaat
     2221 tgcaaggacg tcagatgttc caagcagaat gccgccaaag taaaacagaa tatttgtcat
     2281 cgtaaaacca atgaacgttc cccacagcgc acttttgctt ttcttggaaa acctgttgta
     2341 gtcagcaaca agtggcatcc acgatacggg cattgctata acaaggtcca gtgcggaaaa
     2401 gaacgacaag ccttccccgg gggacgaaat aagagccgta atgtctgccg agtttatcag
     2461 attgataatt attattgcag atgtcccata tgctatccac acggcaaact tgccaatcca
     2521 ccgccttacg acattcagtg gtcctgcaat tacgagaagc gccacaagtg ccccaaatat
     2581 tgctgcccaa aagtagtaag gcaagatact accggtcagc atatctgctg cccttgccat
     2641 tatcataatt tcaaatgtag tccagccgat cagctgcagg atattgagta ttgcaggcag
     2701 ataagagcct ctgatgccaa atgccggtct cattgttatg agcgacggta tcccatgatc
     2761 acttcctatc
//
LOCUS       DASQTF010000037         6652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_193941,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000037 DASQTF010000000
VERSION     DASQTF010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193941"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VE308_00880"
     CDS             complement(<1..370)
                     /locus_tag="VE308_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99553.1"
                     /translation="MASNEGPRILWEQLKGKKVKTNDGHDIGEIKEITQNYIRTEKGM
                     LNKDKSWIPKYLADAYDGKVLWLVIGNDDLAKGYSYTTEPDREQHTHDFEVFKATPYG
                     QKAVQLPDFEQNIRMTEERES"
     gene            816..1589
                     /locus_tag="VE308_00885"
     CDS             816..1589
                     /locus_tag="VE308_00885"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HZA99554.1"
                     /translation="MQELILIKLGGSVVTFKEKPLAANIDAIDSISRALAQLSVPVII
                     VHGGGSFGHYWSVKYSMHTKSANYDVHGVSIVHESMIALNQIIVNSMIRVGLNPYGIP
                     PSALATGHKPIVAKIKHIYAMTQSKLIPVTFGDIVHVEGTKYSILSGDALMAILAKVL
                     RPLRVIFATNVDGIYKDGVSRELVNEIQVAEKRKSIEFFKTSGIDATGGMQRKVTEAF
                     KIASHGMDVVMINGLVPQRILEAAEGTLNVGTVIRGRRS"
     gene            1590..2684
                     /gene="fni"
                     /locus_tag="VE308_00890"
     CDS             1590..2684
                     /gene="fni"
                     /locus_tag="VE308_00890"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HZA99555.1"
                     /translation="MPDTFDAPSDIELIKQRKKEGIDIPLQKNVQAKTTSTYLEYVRL
                     VHNALPEMDYDDIDTSMTFLRKRFSAPIIIDSMTGGTDEATVINGRLGELAEKYGFGM
                     GLGSQRAGLKSEELAATYSIARKNAPNAFLIANIGGAQLSKGFTVDEAKRIVKMIGAN
                     ALVVHLNPLQELIQPEGEPRYKGVLAQISELAKTIDVPLIVKEVGAGISKEVAIKLEM
                     ANVSAINIAGAGGTSWAGVEKLRAESLKDDLKKHLGEMFWDWGIPTAASLMEVRRAVK
                     LPLIASGGLRNGLEVAKCIALGASMAAMAYPFLQTAAQSRENLFAFADTILAELKSTM
                     FLVGAKNIAALASSRYILTGMLAHEVSTRT"
     gene            2681..3691
                     /locus_tag="VE308_00895"
     CDS             2681..3691
                     /locus_tag="VE308_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZA99556.1"
                     /translation="MKADSIKSELESYASTVNDFILTFVEGKPKELYQASSHYIRTGG
                     KRLRPFMVLKGCEMLGGSAERALPAAAAVELVHNFTLVHDDIMDNDEMRHGTSTVHRY
                     YGMPLAILAGDILFSKAFELLTFKCRKAGIPDKSIIDMVEHLSFACTEICEGQAIDVE
                     VASSPKFPSVQHYIDMIGKKTATLFEVSCALGALSSQNHTPSDIRNLSSFGRNIGIAF
                     QLVDDLIGVIGDPKLTGKAAGNDIREGKKTYPILLALKNANKEDTEKIKRVFGTRGKA
                     LNQDIKEAVKIISHIGIEQDVRNEARTYLNEAVKSIEGYGNSDARRALQFSADFVVGR
                     SL"
     gene            3698..5440
                     /gene="gltX"
                     /locus_tag="VE308_00900"
     CDS             3698..5440
                     /gene="gltX"
                     /locus_tag="VE308_00900"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZA99557.1"
                     /translation="MPIDPDTEKFIKIIALQNSVEHGGKAQSDAVIAKFVGLKPELRS
                     QIKLLISEIKAVVQQINALSLADQKSLLEELVPRKVAAKKHVSEQQQQLPPLEGAMQG
                     KVVTRFPPEPNGYPHIGHAKAAIIDEEYARMYSGKLILRFDDTNPLNEKVEYYDAIAE
                     GLKWLGVKPDIIKNTSDDIELLYNYGKRLIELDGAYVCICSQEIIHDLRARGIPCDCR
                     RDPATALDRIGKLFGGSYEQNEAIVRFKGDMADQNTAMRDPALFRIIDCDHPKLGNRA
                     RIWPTYDFAAPIEDSLDGVTHALRTKEYELRNPLYFAILNRLSLRKPYLIEFSRLEFE
                     GIPVSKRKIKPLIENGTIRSWDDPRLPTLAALRRRGFVSDAIRKFVLSLGLTLAETKP
                     PFESLEALNRKIIDPISLRLFFVKNPVEMYVRGGPEMDVVLKNHPTNVNLGVRTVKVS
                     DRFYISEDDATGLKVGDEIRLIELYNVKITSLDWKNGARLIITAEASGNEIRQSTPKI
                     QWIAKNDIIDYKVIIPKKLYMSEEEYNTNSLEISQGFAESFVSHLEPDARVQFVRFGF
                     CRIDGNQIAIMTHR"
     gene            5448..6317
                     /locus_tag="VE308_00905"
     CDS             5448..6317
                     /locus_tag="VE308_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZA99558.1"
                     /translation="MKVARIRDSKVKETYSLISDDGKSIVTRSEIQQQTGIPVPLNIK
                     DFAFRGWLDEVKHHKTNLKFNQKVSDVELLVPIPNPPKIVCLAFNYYDHARDAGLTPS
                     DEPVIFMKPRTALNEPFKDIICPSFVTRLDYEAELAVIIGKETRKVPEDKALDSVFGY
                     MILHDVSARDIQFKDKQFTRGKGIDTFAPCGPWITTKDEISDPQNLDITTKVNGEIRQ
                     NSSSSNMVIPIRRIISTLSVTMTLEPGDIISTGTPAGVAMSMKEPKYLRDGDVVEITI
                     ERLGTIRNRIVYH"
BASE COUNT         1963 a         1406 c         1613 g         1670 t
ORIGIN      
        1 tggactctct ttcttctgtc atgcgtatgt tctgctcgaa atccggaagc tgaactgcct
       61 tctgtccata aggtgttgct ttgaacactt caaagtcgtg tgtatgctgc tccctatctg
      121 gctctgtcgt gtatgaatat ccctttgcaa gatcgtcgtt accgatcaca agccagagaa
      181 ctttgccgtc ataggcatcg gctagatatt ttgggatcca tgacttgtct ttgtttagca
      241 ttcccttttc tgtgcgtata tagttttgtg ttatctcttt aatctcccca atatcatgac
      301 catcatttgt ctttaccttc ttgcccttca gctgttccca caatatcctt ggcccttcgt
      361 tgcttgccat tgtaaaacga tgcaacatat ttgctataaa gattgaagaa aagagttctg
      421 caataggcta tgctcacagt tctggagcga gtcactatac ttcctttttt cacgccttta
      481 ctcctaatat cccaagactc tgactacgtt agttaaacaa caataatgct tcatctttag
      541 cttggtctgt aagtgactta ggagcagctt gtacttttct tatatgtaga ttctttgaag
      601 aagatgaaag tgtgcttcga tctaatcctt gagattttct tttcaatgct tatgtgagtc
      661 gcaggcaact atttgagcta ctcaagctca aattggtgca aatgcttttc cgatgtatct
      721 gattccgata gtactcgtag tatcatgtag tagggtggag aagtgaaaaa gcaagttaaa
      781 acttaaattc acttcaaaaa gtatgctttt tgttgttgca agaacttatc cttatcaagc
      841 tgggcggctc tgttgttacc tttaaggaaa aaccgctagc agctaatatc gatgcgatag
      901 atagcatctc acgcgctctg gcgcaattaa gcgttcctgt cataattgtg cacggcggcg
      961 gctcgtttgg ccactactgg tcggtaaaat atagcatgca taccaagtcg gccaactatg
     1021 acgttcatgg agtttccata gtgcacgaat caatgatcgc actcaatcag ataatcgtca
     1081 attccatgat aagggtggga cttaacccat atggcatacc accgtcggct cttgcgacag
     1141 gccataagcc aattgtagca aagataaagc acatctatgc aatgacccag tcaaagctaa
     1201 taccagtaac gtttggcgac atagttcatg ttgaaggtac caaatattct atcctatccg
     1261 gcgatgcact aatggcgatt ttggcaaaag tactgcgacc gctaagggtc atttttgcta
     1321 ccaatgtcga tggcatatat aaagacggcg taagcagaga acttgtaaac gagatacagg
     1381 tcgcagagaa gagaaaatcg attgagttct tcaagacatc cggcatcgac gcaactggtg
     1441 gaatgcaaag aaaggttaca gaagcattta agattgcatc acacggaatg gatgtagtga
     1501 tgataaatgg gcttgtgcct caacgcattc tagaggcagc agaaggcacg ctaaatgttg
     1561 gcacagttat aagggggaga aggagttagt tgccagacac ttttgacgcg ccctctgata
     1621 tagagttaat taagcagcgc aaaaaggaag ggatagacat cccacttcag aagaacgtac
     1681 aggcgaaaac tacttctaca tacttggaat acgtcaggct agtgcataac gcgctgcctg
     1741 agatggacta tgatgatatt gacacttcga tgacttttct taggaaaagg ttttcagcac
     1801 cgattataat cgactctatg actggcggca ccgacgaggc aactgtcatt aacggcaggc
     1861 tgggtgagct cgcagaaaag tatggctttg gcatggggct tgggagccag agggccggcc
     1921 tcaaaagtga agagcttgct gccacctatt cgatagcaag aaagaatgcg ccaaatgcat
     1981 tcttaatagc aaacatcggt ggagcgcaac tttccaaagg gttcacggta gatgaagcta
     2041 aaagaatagt gaaaatgatt ggggcaaatg cgctggtggt gcaccttaac ccgctgcaag
     2101 agcttataca gccagagggc gagccgcgct ataagggagt tcttgcccag atttcagagc
     2161 ttgccaagac catagatgta cccctgatag tcaaggaggt gggcgccgga atttcaaagg
     2221 aggttgccat aaaactggag atggccaacg tttccgcgat caatattgca ggggctggcg
     2281 gcactagttg ggccggtgtt gaaaagttac gggcggagtc tctcaaagac gaccttaaga
     2341 agcaccttgg tgagatgttc tgggactggg gcattccgac tgcggccagc ctcatggaag
     2401 tcaggcgcgc agtgaaattg ccgctcattg catcaggtgg tctgcgcaat gggctggaag
     2461 ttgcaaagtg catagcgctt ggtgccagca tggctgcaat ggcatatcca ttccttcaaa
     2521 cagctgcaca gtcacgcgaa aacctgtttg catttgccga tacaattctt gcagagctta
     2581 agagcactat gttccttgtt ggagccaaaa acatcgctgc gcttgctagc tcaagatata
     2641 tattgacagg aatgctagca catgaggtta gtacccgtac atgaaggcag acagcatcaa
     2701 aagcgagctg gaatcttatg cttctaccgt gaacgacttt atacttacat tcgttgaggg
     2761 caagccaaaa gagctttacc aggcttcgtc acattatatc cgcacgggag gcaagcgatt
     2821 gcgaccattc atggtgctca agggctgcga aatgctaggt ggaagcgccg agagggctct
     2881 ccctgcagcc gctgcagtcg agcttgtaca taactttaca cttgtacatg acgatatcat
     2941 ggacaatgat gaaatgcggc atggcacttc aactgtgcac agatactatg gtatgcctct
     3001 tgcaattctt gccggcgaca ttctattctc aaaagcgttt gaactattga cgtttaaatg
     3061 cagaaaggcc ggaattccag acaagtcgat aattgacatg gtggaacatc tttcatttgc
     3121 atgcacggaa atatgcgagg gacaggcaat agacgtagaa gtggcatcaa gtcctaagtt
     3181 tccaagtgtg cagcattata tcgacatgat tggcaaaaag actgctacgc tctttgaagt
     3241 atcgtgcgca cttggcgcgc tgtcatctca aaatcacacc cctagcgata tcagaaatct
     3301 ctcttcattt ggccgcaaca tcggtattgc tttccagttg gtagatgatc tgataggtgt
     3361 aataggcgac cctaagctga caggcaaggc cgctggcaat gacattcgag aaggcaagaa
     3421 gacatatcca atactacttg ctctgaaaaa cgccaacaaa gaagacacag aaaaaatcaa
     3481 gagggtattt ggtacaagag ggaaggcctt gaaccaagat atcaaggagg ctgtcaagat
     3541 catctctcat attggcatag agcaggatgt gaggaatgaa gcaagaacgt acttaaatga
     3601 agcagtcaag tcaattgaag gttacggtaa ctctgatgcc agaagagctc ttcagttctc
     3661 ggcagatttt gtagtgggca ggagtctata gtagtacgtg ccaatcgatc cagatactga
     3721 gaaattcatc aaaatcattg cattacagaa ttcagttgag catggcggca aggcacaatc
     3781 tgatgcagtg atcgccaagt ttgtcggctt aaagccagaa ttgcgtagcc aaatcaagtt
     3841 acttatctct gaaatcaagg cagttgtgca gcaaataaat gcactttcac ttgcagatca
     3901 aaaatcgctt cttgaagagc ttgtaccaag aaaggtggct gcaaaaaagc atgtatcgga
     3961 acagcaacag caactgcctc cgcttgaagg agccatgcag ggcaaagtgg ttactcgatt
     4021 tccgccggag ccaaacggct acccacatat tggacatgca aaggcagcta taattgacga
     4081 agagtatgcc cgaatgtaca gcggaaagct gattctccgc tttgatgata caaatccgct
     4141 caacgaaaaa gtggaatatt atgacgccat agctgagggg ctgaaatggc ttggagtcaa
     4201 gcctgatatt attaagaata cttcagacga cattgagctt ttgtataatt atggaaaaag
     4261 gctgatcgag ctagacggcg catacgtatg catctgtagc caagagataa tacatgacct
     4321 ccgagcaagg ggcatcccct gcgattgcag gcgcgatcct gcaaccgcgc ttgataggat
     4381 tggaaaattg ttcggtggct catatgagca aaatgaagcc attgttcggt tcaaggggga
     4441 catggccgac cagaatacgg ctatgcgtga cccagcgctt ttcaggataa tagactgtga
     4501 tcaccctaag ctgggcaaca gagcgcggat ttggcctacc tatgactttg cagcgccaat
     4561 tgaagacagc ctcgatggcg taacgcatgc tttgagaacg aaagaatatg agcttcgcaa
     4621 tccactctat tttgcaattc ttaataggct cagtttaaga aagccatatc ttattgaatt
     4681 ttcaaggcta gagtttgaag gaattccagt ttcaaagcga aagataaaac cacttataga
     4741 aaatggcacg atcagaagct gggatgaccc gcggctccca acacttgctg cgcttagaag
     4801 gaggggcttt gtgtccgatg cgatacgcaa atttgtgctg tcgctgggcc ttacgcttgc
     4861 agaaaccaaa cctccttttg aaagcttaga agctttaaac cggaagataa tagatccaat
     4921 atcgctgcgg ttgttctttg tgaaaaaccc tgtcgaaatg tatgtcaggg gcggtcccga
     4981 aatggacgtc gtactcaaga accatcctac taatgtcaac cttggggtga ggacggtgaa
     5041 ggtaagtgac cggttctata tctctgaaga tgacgcaaca gggctgaagg taggggatga
     5101 aattcgactc attgaattgt ataatgttaa gataacgagc ctggattgga agaatggtgc
     5161 ccggttgata ataacagctg aggctagtgg taacgagatc cggcaatcca cgccgaaaat
     5221 acagtggatt gcaaagaatg atataattga ctacaaggtc ataattccca agaaactcta
     5281 catgagcgag gaggagtaca atactaacag ccttgaaatt tcgcaaggtt ttgcagaatc
     5341 ttttgtatcc caccttgagc ctgacgcacg ggttcagttc gtgagatttg ggttttgcag
     5401 aatcgacggt aatcaaatcg caataatgac gcacaggtaa atggacaatg aaggtagcaa
     5461 gaatccgaga tagcaaggta aaggaaacat acagcctcat atccgatgat ggaaaaagca
     5521 ttgtcacaag gtcggaaata cagcagcaga ccggcatacc tgtccccctc aacatcaagg
     5581 actttgcgtt ccgaggatgg cttgacgagg tcaagcacca caagaccaat ctgaaattca
     5641 atcagaaagt gagcgatgtc gagctgttag tgccgatacc gaatcctcca aagatagtat
     5701 gccttgcctt taactattac gatcatgcgc gtgacgcagg gctcacccct tcagatgagc
     5761 ccgtgatatt catgaagcca aggaccgctc ttaacgagcc cttcaaagac attatctgcc
     5821 cgtcattcgt aactcgcctt gactatgagg ccgaacttgc agttatcatt ggcaaagaaa
     5881 ctaggaaggt gccagaagat aaagcacttg attctgtctt tggatacatg atactgcatg
     5941 acgtgtctgc aagggatatc cagttcaagg acaagcagtt tactcgaggc aagggaattg
     6001 atacttttgc tccatgcgga ccttggataa cgaccaaaga tgagatctca gatcctcaga
     6061 accttgacat aacaaccaag gttaatggcg aaatacggca aaactcatca agttctaata
     6121 tggttatccc aattagacgg ataatttcca cacttagcgt gaccatgacg cttgagcctg
     6181 gtgacatcat ttcgacagga acaccagctg gagtagcaat gtcaatgaaa gagccaaaat
     6241 atctaaggga tggagacgtg gtcgaaataa cgatagaaag actgggaacg ataaggaacc
     6301 gcatagtata ccactaacat caagcagagc attgctttca aggacgactt gacatggatg
     6361 gtcgtcgtat gtcaaagaca ttctttgctg acatgaacga gggctggata ggagaaaaaa
     6421 cctgctgaga aggaaatgcg aagaattatt caagtcaaat gggtatgagc cgaaaggtat
     6481 ggctggaatg tgataggata tttgattagc ggagaggatt tttaatctct agtgacacag
     6541 aaacctaaaa cgacgaaata taatgtggca gaggtgatgg actgcaattt attccacacc
     6601 gtaatagaag atatgtgata ttagtctgct tggctgcatg aactactgtc tt
//
LOCUS       DASQTF010000038         6228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1024658,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000038 DASQTF010000000
VERSION     DASQTF010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024658"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..973)
                     /locus_tag="VE308_00910"
     CDS             complement(422..973)
                     /locus_tag="VE308_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HZA99559.1"
                     /translation="MPRNPGGIACYAYIIRNKEGHLLDESCGLAAEPNSPSSTNSSAS
                     YTALIRALEWLIKKGYSNDIIKVYGNSKLVISQVNEGEVAISSNKNYISKNTLSLYTK
                     VMKLKSKFYYISFELNNDNENRHLDDKEVEELSLLAYIEAKTKILQQSGSGGLNNSNN
                     KYRQELKKKLFSTAAELMMTAAK"
     gene            complement(1276..1557)
                     /locus_tag="VE308_00915"
     CDS             complement(1276..1557)
                     /locus_tag="VE308_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99560.1"
                     /translation="MADIKKTIKFHGLEVANIIVRYSQREVNKPDAIKIQEFDARKDP
                     QICETVNIQVVSDFVTITFYKNESENIVIRRELIPAFTVEHIWVSDLQQ"
     gene            complement(1723..2157)
                     /locus_tag="VE308_00920"
     CDS             complement(1723..2157)
                     /locus_tag="VE308_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99561.1"
                     /translation="MPPNHTKDEIEKLSDSQDFEGSKKKTTEKSKVTTKASTFDLTKA
                     GSTPTGITDILDNPDVAVWSKGIANKTNTPADKVQEPYHIKSTATHIEYREGEEKQSH
                     FQQTVDPANKEDVAKAIDLGTSLTDEATSFVQRSKKATSSKE"
     gene            2361..2852
                     /locus_tag="VE308_00925"
     CDS             2361..2852
                     /locus_tag="VE308_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZA99562.1"
                     /translation="MKIGLIADTHDNFKTIEGAVRIFREKGIDYVIHAGDITTPEAVE
                     AFAGLKLIGVLGNNDVDKQGLDNAFEKIGGELRGELGEIEEDDLLIAIYHGTDFKKRE
                     ALIQSGKYNVVVYGHTHKVDNKVVGKTIVINPGTANGWFFGYKATAAIFNTVSKECDF
                     VEL"
     gene            3056..3262
                     /locus_tag="VE308_00930"
     CDS             3056..3262
                     /locus_tag="VE308_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99563.1"
                     /translation="MSVRETVVSKGNFTPGQSWGALRKAWKGYRIAKVQGDNTKMSEY
                     ATKIRKLQGELGISVASFPHLGIN"
     gene            complement(3289..3726)
                     /gene="trxA"
                     /locus_tag="VE308_00935"
     CDS             complement(3289..3726)
                     /gene="trxA"
                     /locus_tag="VE308_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZA99564.1"
                     /translation="MSTDEELEAIKHKKLAELQKEATTKAMMSSITEPIVLTDSNFAN
                     EVTKYPIMLVDFWAPWCGPCRIVSPIIEQLSREYSGKVAFGKVNVDENQRIAASFGIQ
                     SIPTLMIFKGSKAVDVIIGAMPKAQIELKLKQQLLSKNDSIYR"
     gene            3822..4442
                     /locus_tag="VE308_00940"
     CDS             3822..4442
                     /locus_tag="VE308_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZA99565.1"
                     /translation="MVENDERANIMAEQQEGKPGEQEEVEALRDELESVKSELRKARE
                     SSESSLNKMRYLMADFDNYRKQMEKQLASKAESIKAELLLKFLNIRDDYLRALSMARQ
                     TKSEQGVVIEGLEGILKNIDSLLASEGVREIEAIGTPFDPNVHDAIAYSARDDLAENT
                     VTAEIRKGYMLNGRVLRPSLVEISKIVKNSVNDTKEVDIQGEQGGG"
     gene            4396..>6228
                     /gene="dnaK"
                     /locus_tag="VE308_00945"
     CDS             4396..>6228
                     /gene="dnaK"
                     /locus_tag="VE308_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZA99566.1"
                     /translation="MTLKRWIFKENKEADNMGKIIGIDLGTSNSAASVMVGGKPTIIQ
                     AAEGTSIGGKAFPSVVAFTKDGQLIVGEPARRQMISNPEGTVIAAKRKMGTDFKFKVY
                     GKEYAPQQISSFILQKIKRDSEAFLGETVDKAVITVPAYFNDNQRQATKDAGEIAGLQ
                     VVRIINEPTAASLAYGLDKASKDLKIMVFDLGGGTLDVTIMEMGGGVFQVRSTSGDTQ
                     LGGTDMDNTLVEYIVQEFRRQSGLDVKNDKAAMMRIREAAEKAKIELSNVVTTEINLP
                     FLAYDPSSGPKNLILQLTRAKLEELLRPIIERCRQPMMQALQDPKMTPADIDKIILIG
                     GPTRMPMVRQFVESVMGKEPERGIDPMEAVAMGAAVQGAVIAGDVSTDILLVDVTPLT
                     LGVEVLGGIKEPLIERNTTIPTKKSKVFTTAADYQTAVTVHVVQGERSMAADCVSLGM
                     FNLSGIPPAPRGVPQIEVAFDIDANGILNVTAKDIATQKEAKITITANNKLSKDEIER
                     LKKDSEQFADADKKKKEEAEIRNEADNLIYAADKLVKQDLKDKVSSDQVEKVNKLTQE
                     IRDAIASNNAENIKAKIQELKSVLGEISTQAYQSAGASSAQAGAA"
BASE COUNT         1858 a         1237 c         1383 g         1750 t
ORIGIN      
        1 ccgcagctgt cgcatttgta ttcttggtaa taatcgccag tcagtcggtt tgtatctgga
       61 tctgcgcttg ttgctgatgc tgctcctcct gtcggcttca ttttcccgat tccgcattta
      121 ggacaagtat caaattcttc ccttgccata ggggacaagc ttgtatttag tgaatttaac
      181 cttagcgata gtacgaaaaa acagctacct tattgtttca aacgatgcga tcagtagcag
      241 aagaaaaagc gtttctacat atccacgctg ccgtttttct taactcttcg ggatcgtctt
      301 agatgtctct agagcatcat tatcatcata agagaaggat gttattatag aaaaatgaat
      361 gtcaagaact ttgttttgat cttttcacta tttcttaaag gatcaggtcg cttttgttta
      421 tttattttgc tgccgtcatc atcaattctg cagcggtaga gaataacttt ttcttcagtt
      481 cttgtctgta tttattgttg ctattattaa gcccaccact tccgctttgt tggagtattt
      541 tcgtttttgc ttctatgtag gctaaaaggg acagttcttc gacttcttta tcatcaagat
      601 gcctattttc attgtcgtta ttcaattcaa atgatatata atagaatttt gatttcagct
      661 tcattacttt tgtatacagt gaaagagtgt tttttgatat atagttcttg ttgctgctaa
      721 tagcaacttc cccctcatta acttgagaaa ttacaagctt cgaattgcca tatactttta
      781 tgatatcatt actataacct tttttgataa gccattcaag cgctcttatc aaggcagtat
      841 aactggcgct actattggtg gatgatggag aatttggctc cgctgctaga ccgcaagact
      901 catctaataa atgaccctct ttattcctga taatgtaagc ataacacgct atacctccag
      961 ggtttcttgg caaacattgt cctagaaagc gtactgttac tatttccttc atgttaagtt
     1021 ttttgcatta cgcgcattta atgcatatgt atctctgtct ctttttagaa gatgaaaaaa
     1081 gaaaaagagc aagaattttt ccatcatgca ctccttcttt caacttttgc ttttgaaccc
     1141 ctggctttat gaatggcaag aattagaaaa tcttctttgg tctagccaat gaaattgaat
     1201 atgaagaaaa attaggagtt cgggagtata agggacgaag aaaaagaata gctcagaaaa
     1261 agatgatggt tgaaatcatt gttgcaagtc gctaacccaa atatgttcca cagtaaaggc
     1321 tggaattagt tctctgcgaa taactatatt ctcagattca tttttgtaaa atgtaattgt
     1381 aacaaaatct gacacaactt gaatatttac agtttcgcaa atctgtggat cttttcttgc
     1441 gtcaaactct tggatcttta tcgcatctgg cttgtttacc tctctctgag aatacctaac
     1501 aattatgtta gctacttcta agccatggaa ctttattgtc tttttaatat ctgccatatc
     1561 ttgatgccta atacagtatg cagcaatgaa atactaataa attttacaat ttttcgagaa
     1621 tatatcccgt gtcgagaggg tgtgcgagca tctttgtttg agaggttagg aaaaagtaca
     1681 tgtgtatatc catcatataa tcagacaatt ctaccaaggc ttttactctt tggaggatgt
     1741 tgcctttttg cttctttgaa caaagcttgt tgcttcatca gtcagagaag ttcctaagtc
     1801 tatagctttg gctacatctt ctttgttggc tggatccacg gtctgctgaa aatgactttg
     1861 tttttcctcc ccttctctat attcaatatg ggtggcagtc gatttaatgt gataaggttc
     1921 ctggactttg tcggccggcg tattagtctt attcgctatt cccttagacc atactgcaac
     1981 atccgggtta tctagtatat cggttatccc agttggtgtg ctaccagctt ttgttaggtc
     2041 aaaagtactc gctttagtcg ttactttact cttttctgtc gtcttttttt tgctgccttc
     2101 aaagtcttgt gagtcactta gcttttcaat ttcatctttt gtgtgattag gaggcatttc
     2161 tgtatcaact aatcatggtt tacatacatt atagagccat tcaacactaa gtcttcagct
     2221 aaccaagaga tcgaatgaaa tccagcagct ttagaatcta atccaagcgg aattatctat
     2281 attgttattt caacctctag ctttccttta gattagttct tagctttata ttatatgtac
     2341 ttcattcttt aggaaaagaa gtgaagattg gactaattgc agacacccat gacaatttca
     2401 agaccataga aggtgctgtc aggatattta gagagaaagg aatagactat gtaattcatg
     2461 ctggcgacat aactactcct gaagcagtag aagcttttgc aggactaaaa ttgatcggcg
     2521 tattgggaaa taatgatgtg gacaaacaag ggcttgacaa tgcttttgaa aaaataggag
     2581 gggagcttag aggtgaactt ggcgagattg aggaggatga cttgctaatt gccatctatc
     2641 atggaactga ttttaaaaaa cgagaagctc tgattcaatc aggcaaatac aatgttgtag
     2701 tttatggtca cactcacaag gtagataaca aggttgttgg aaaaacaatt gttattaatc
     2761 ccggaaccgc taacggttgg ttctttggtt acaaggcaac tgctgcaata tttaacacag
     2821 taagcaagga atgtgatttt gtagagctat aataagtagg gcataatgcg catgtatata
     2881 gacttgtaga agagaaagag actcaagttc tctatcactt tatctcaaat atcaaaatct
     2941 gcatgtgtta tctcatggct tgctcggtgc ccaactgttt tcaccacaaa acctgagaaa
     3001 attgactacc ggtgctaagt accaactagg gatctatctc agcatgacag caataatgtc
     3061 agtccgcgaa acggttgtat caaaaggcaa ctttacgcca ggccaaagtt ggggcgcact
     3121 gaggaaggca tggaagggat acaggatcgc caaggtacag ggcgacaaca ctaagatgag
     3181 cgaatacgcg accaagatca gaaagctcca aggcgagttg ggaataagcg ttgcttcatt
     3241 cccgcactta gggataaact aagtgcaacc tttttctttt tctactaact atctgtaaat
     3301 tgaatcgttc tttgacaata gttgttgttt tagcttcaac tcgatctgtg cttttggcat
     3361 agcgcctatg attacgtcga ctgccttact gcccttaaat atcattaggg taggaatact
     3421 ctgtatacca aatgaagccg ctattctttg attctcgtct acgttaacct tgccaaatgc
     3481 taccttgcca gaatattctc gagataattg ttcaattatt ggcgagacaa tcctacatgg
     3541 gccacaccat ggcgcccaaa agtccacaag catgatggga tattttgtaa cctcgtttgc
     3601 aaagttagaa tccgtgagca ctattggctc ggtaatggat gacatcattg ccttggtagt
     3661 ggcctccttt tgcagctcgg ccagcttctt atgcttgatc gcctcaagtt cctcgtctgt
     3721 gctcatacac accaatcatt ggtacaaata ggctatattt cttacgagcg atatctaatt
     3781 attagatggg ctcctttttt ggaagcagaa ggtgattcac tttggtcgag aatgatgaaa
     3841 gagccaatat tatggcggag cagcaagaag gcaaaccagg ggagcaagaa gaagttgagg
     3901 cgctaagaga tgagctcgaa tcagtcaaga gcgagctgag aaaagcaaga gaatcctctg
     3961 aaagcagcct caacaagatg aggtacctaa tggcagactt cgataactac cgcaagcaga
     4021 tggaaaagca gcttgcctca aaggcagaat cgatcaaggc agagttgttg ctcaagtttc
     4081 tcaacatacg tgacgactac ctccgagcgc tctcgatggc taggcagacc aagtcagagc
     4141 aaggagtagt aattgaaggg ttggaaggaa tactcaagaa tattgactcg cttcttgcct
     4201 cagaaggagt aagggagata gaggcgattg ggacaccttt tgacccaaac gtgcacgacg
     4261 ctatagcata ttctgcacga gatgacttgg cagagaatac agtgactgcc gagataagaa
     4321 agggctacat gctcaacggc agggtgctaa gaccaagcct tgttgaaatt tcaaagatag
     4381 ttaaaaatag tgttaatgac actaaagagg tggatattca aggagaacaa ggaggcggat
     4441 aatatgggaa aaataatagg tattgatctt ggtactagca attcagcggc ttcagtcatg
     4501 gtaggcggca agccaactat aatccaggca gcggaaggca cctctatagg cggcaaagca
     4561 ttcccgtcag tggtagcatt taccaaggat gggcaactca tcgttggcga gccagctcgc
     4621 cgtcagatga tttcaaaccc tgaaggaacc gtcattgcag caaagcgaaa gatgggaaca
     4681 gattttaaat tcaaggtata cggcaaagaa tacgctccac agcagatatc atcattcatt
     4741 ctgcaaaaga tcaaaaggga ctctgaagcg ttcctaggag aaacagtcga caaggcagtc
     4801 attaccgtgc cagcatactt taatgataat caacgccagg cgaccaagga cgcgggagaa
     4861 attgcaggtc ttcaagtagt taggataatt aacgaaccca ctgctgcatc gcttgcctat
     4921 gggcttgaca aggcaagcaa ggacctcaag ataatggtct ttgatcttgg aggaggaaca
     4981 ctcgatgtta cgataatgga aatgggaggc ggtgtgttcc aagttagatc tacctcaggt
     5041 gacacccagc ttgggggcac agatatggat aatacgcttg tcgaatacat cgtacaggaa
     5101 ttccgcaggc aatcgggcct tgatgttaaa aacgacaagg ctgcaatgat gaggatcagg
     5161 gaggccgcag agaaggccaa gatagagctc tcaaatgttg ttacgacaga gatcaatttg
     5221 cctttcttgg catatgaccc atcttcagga ccaaagaacc taatactaca gctgacaagg
     5281 gcaaagcttg aggaactgct ccggccgata attgagcgct gcagacagcc tatgatgcag
     5341 gcgttgcagg atcctaagat gacgcctgca gacattgaca agatcatctt gattggtggc
     5401 cctacaagga tgcccatggt gaggcagttc gtagagtcag ttatgggcaa ggaacctgaa
     5461 cgggggattg atccaatgga ggcagtggca atgggtgccg cagtccaagg tgcagtcata
     5521 gcaggagacg tttcaaccga tatcctgctt gtggacgtaa cgccgctcac attgggtgtc
     5581 gaagtactag gcggaataaa ggagccgcta atcgaacgca acactacaat tccaacaaag
     5641 aaaagcaagg tgttcactac cgctgcagac taccagactg cggtaacagt acatgttgtg
     5701 cagggagagc gatcgatggc agctgactgt gtttcacttg gcatgtttaa cctatctggc
     5761 attccgcctg ctccaagggg cgtgccccaa attgaagttg catttgacat agacgcaaac
     5821 ggcatattaa acgtcactgc aaaagatatt gctacgcaaa aggaagcaaa gatcacaata
     5881 actgcaaaca acaaactatc caaggatgaa attgaaaggc taaagaaaga ttcagagcaa
     5941 ttcgctgatg ccgacaagaa gaaaaaggaa gaggcagaga taagaaacga agcagacaac
     6001 ctcatttacg ccgctgacaa gctggtgaag caggatctta aagacaaggt aagttcagat
     6061 caagtagaga aagtgaataa actaacacag gaaatccgcg atgctattgc aagcaataac
     6121 gcagaaaaca tcaaggctaa gatacaggaa ttgaagagcg tcttgggaga aataagcacg
     6181 caggcatacc agagcgccgg tgcctcatcg gcacaggccg gtgcagca
//
LOCUS       DASQTF010000039         4270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_285331,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000039 DASQTF010000000
VERSION     DASQTF010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285331"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VE308_00950"
     CDS             complement(<1..302)
                     /locus_tag="VE308_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99567.1"
                     /translation="MKRAKILADSSVKATSPKGVKSQPAPRLTQQDKVSSSVAQVDQS
                     TQSHELTAGAGSYSSPGSGPNASVGNTTISLTGSGTDYSKVDGPIDPSFKTKRQSAG"
     gene            complement(574..927)
                     /gene="pth2"
                     /locus_tag="VE308_00955"
     CDS             complement(574..927)
                     /gene="pth2"
                     /locus_tag="VE308_00955"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HZA99568.1"
                     /translation="MELKQVIVVRKDITMGRGKLAAQVAHAAVLAVEIARLTRQDWLK
                     LWFLTGQAKVVLRVENLAELVRLECRAQTMGLPAARVQDSGLTQIPSGTVTCIGIGPG
                     PEELIDKVTRHLRLL"
     gene            982..2145
                     /locus_tag="VE308_00960"
     CDS             982..2145
                     /locus_tag="VE308_00960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZA99569.1"
                     /translation="MEQAYDVIIAGGSVSGLFAAREIASRGHSVLVLEEDSEIGTPEH
                     CGGLVSIDAMRNLGVVPRSSTIQNQVRAAKICSPSKSFQLNTAGQRVVVIERREFDKQ
                     VAFQSTRAGATIRTKCSLLGTKYTADSPDECFRLVTSDGRLQCQYLVDARGIASLVQN
                     DRSGILVAAQYEVCAPWIQKDTVIVEFDSIKYPGFFAWIIPIGEGRGKVGVAGRNINA
                     ARSIASYLASRGNQYSVIRKIFAPIWVKGPIDEFVNGRTITVGDAAGQTKPTTAGGIY
                     TCGLAGIYAGRAISEAIEGKDDGYLSDYPKNWIRLFGKEFRNMVLFRKVLERLDNHSL
                     DMIFSSITPGDMDRVSENGDFDFHAISVRKLLGVTGLKIFRTLLDNEARRLLQ"
     gene            2184..2363
                     /locus_tag="VE308_00965"
     CDS             2184..2363
                     /locus_tag="VE308_00965"
                     /inference="COORDINATES: protein motif:HMM:NF019374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HZA99570.1"
                     /translation="MSKPLVLPVCTSCGRAVMPNDQCVKFYCPSCGYVIIWRCESCRD
                     FSRQYKCVGCGFEGP"
     gene            2350..2646
                     /locus_tag="VE308_00970"
     CDS             2350..2646
                     /locus_tag="VE308_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HZA99571.1"
                     /translation="MRVHNMARLVARIKMLPAEAESDVENIVTTLRQNPPNGIVMKQS
                     AKEPIAFGLNAVVADFLIDDAEGEMDRLEHSIKGIPGVGEVEVIFVSRQSVSIK"
     gene            2690..>4270
                     /locus_tag="VE308_00975"
     CDS             2690..>4270
                     /locus_tag="VE308_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZA99572.1"
                     /translation="MKKSRLSSGEAHPKIKLRVAEAKYRDVGKRRARIDALSMNKLAI
                     KPGEVVELIGKRSTPVTVWPAEAQENGREIIRIDGQTRKNVGVGLNDLLTVRKIRSKA
                     AKSVFLAPLSPNTISADTQFRDFVRNRLRGFPINEGDEISVGVLGNSIDFVIKKIIPR
                     TISKIERSTKVNIVAAASIDKKPRVTYDEIGGLKEQIDRLREIVELPLRHPEVFAKLG
                     IEPHSGILMYGSPGCGKTLIAKALASESAANFYIINGPEIVNKYYGETEARLRDIFKE
                     AKETAPSIIFIDEIDAIAPKREEAFGDVEKRVVAQLLALMDGMSDRGNVIVLGATNRP
                     ESIDPALRRPGRFDREIEIGVPNAQGRFEILGIHTRGMPLSQDTDLQELASELNGYTG
                     ADIKALCREAAMKALKRYIPEIDSGSGEVPPDTLRLMDIRSKDFREGMKEIIPTAMRE
                     FYVEVSRIKWEDVGGLYNAKRVLHDNLIMSIKEPDSFSRMGVRAPRGALLFGPPGCGK
                     TLIAKSLATISSANIIVVR"
BASE COUNT         1244 a          881 c         1083 g         1062 t
ORIGIN      
        1 cctgcagact gtctctttgt tttaaatgaa ggatcaatag gaccatcaac ctttgaataa
       61 tcagttccac ttcctgtcag ggatatagtt gtgttgccaa cggatgcatt agggccgctc
      121 cccggggaag agtatgatcc ggcaccagca gtcaattcat gactctgggt ggattggtca
      181 acttgggcga cagaggaaga gactttatcc tgttgtgtca gcctgggggc tggttgagat
      241 tttaccccct tgggtgaggt cgctttcaca gaagaatctg ccaagatctt cgctctcttc
      301 acaacgcaga gctgattatg aacatccttc atgctttgca acgtgagagg attactgtga
      361 ctcaagagag acccacaatc ggcacaaatg tattcagtgg gtgaagacac cattgttatt
      421 caaagtcaac catttatcaa tatttggatt tccataaagg tgaactgagc cttgccataa
      481 tttgatcaga ctatgtagta cccatatgct tgtcggggga tcaaaagaag aatttcaaaa
      541 cacctgtaat cacaacgaag tgttggtcct gatctataat aatcttaagt ggcgtgtcac
      601 tttatctatt agttcctctg gtcccgggcc aattcctatg caagtaactg ttccggatgg
      661 gatttgggtg agtccactat cttgaactct ggctgcaggg agacccatgg tctgggcgcg
      721 gcattcgagg cgaacgagtt cagcaaggtt ctctacccgt aagacaactt ttgcctgtcc
      781 agtcaagaac cagagtttca gccaatcctg cctggtaagc cgcgctatct ccactgccaa
      841 tactgccgca tgagcaacct gggcagccaa ctttcctctt cccattgtaa tatcttttct
      901 aacaacaatg acctgcttta gttccactca aacctctggc aatcatttat taacctaata
      961 aaaaggatcc gtaatcatcc tatggaacaa gcctatgatg tcattattgc tggtggcagc
     1021 gtttccgggc tatttgctgc cagagaaatt gcttctcgag gacattcagt acttgtttta
     1081 gaggaagatt cggaaatagg aactcccgaa cattgtgggg gattggtcag catcgatgcc
     1141 atgaggaatc ttggggtagt cccaagaagt agtacaattc aaaatcaagt cagggctgca
     1201 aaaatttgct cgccttctaa aagttttcag ctaaacacgg cagggcagag agtcgtagtc
     1261 atagaaagac gcgaattcga caagcaggtt gcttttcagt cgacacgagc aggggctact
     1321 ataagaacaa agtgttccct tttgggtaca aaatatacag cagactctcc ggacgaatgc
     1381 tttcgattag tgacttccga cggtaggctt cagtgccagt acttagtaga tgcgcgaggg
     1441 attgcctcat tggtgcaaaa cgaccgatct ggcattcttg ttgcagctca atacgaagta
     1501 tgtgcaccat ggatacaaaa ggacacagta atagtggaat tcgacagcat aaagtatcct
     1561 ggtttttttg cgtggatcat cccgatagga gaggggagag gaaaggtcgg agtagcaggt
     1621 agaaatatca atgctgcgcg ctccattgcg tcatacctgg ccagcagggg aaaccaatat
     1681 tctgtaattc ggaaaatatt cgctccaatc tgggtgaaag gaccgattga cgaatttgtt
     1741 aatggaagaa ccataaccgt tggagacgcg gcagggcaaa ccaaacccac aacagcagga
     1801 ggcatttata cgtgtgggct ggcaggaatc tatgctggga gggccatctc tgaagctata
     1861 gaaggcaaag acgatggtta cctgtctgac taccctaaaa attggatcag actattcggg
     1921 aaagaattcc gaaacatggt actatttaga aaggttcttg aacgtctaga caaccactct
     1981 ctggatatga ttttttctag tattacccca ggagatatgg acagagtttc ggagaacggg
     2041 gactttgatt ttcatgcaat atctgtaaga aaacttctag gcgtcaccgg tttgaaaatt
     2101 ttcaggacgc tcttagataa cgaggctagg aggttattgc agtaagcaag gtataaattc
     2161 ctatggctaa tcaagatttg tctatgtcaa aacctctggt tctgccggtt tgtacctcat
     2221 gtggaagggc cgttatgcct aatgatcaat gcgtcaaatt ttactgtcct agttgcggct
     2281 acgtaattat atggcgctgc gagagctgca gagatttttc caggcagtac aagtgcgtcg
     2341 gatgcgggtt tgagggtcca taacatggcg cgtcttgttg ccagaataaa gatgctaccc
     2401 gcagaggcag agtcggatgt ggaaaatatt gttacaacat taaggcagaa tccgccaaat
     2461 ggaatagtga tgaaacaatc tgctaaagaa ccaattgcct ttggattaaa tgcagtagtc
     2521 gcagactttc ttatagatga tgctgaagga gaaatggata gactagagca ttcaatcaaa
     2581 ggcattccgg gcgttgggga agtcgaggtg attttcgtca gcagacagtc tgtgagcata
     2641 aagtgattag gaaaatgacg acctagatca aacatgagtg atagcatcat tgaaaaaatc
     2701 tcgtcttagc agtggcgagg cccaccccaa gatcaagtta cgtgtggcag aggcgaagta
     2761 tcgcgacgtg ggcaaaaggc gagcacgtat cgatgcacta tcaatgaaca aactagccat
     2821 taaaccaggc gaagtagtag agttgatagg caagcggagc actcctgtaa cagtgtggcc
     2881 tgctgaagcg caggagaacg gacgggagat aatccgaatc gatggtcaaa cccgtaagaa
     2941 tgttggggta gggctcaatg acctgctaac cgtgcgcaag atccgttcga aggcagcaaa
     3001 atcggttttc ctagcgccgc tcagtcccaa cacgatcagc gctgacaccc aattccgcga
     3061 ctttgtaaga aatagactca gaggttttcc gattaacgag ggagatgaaa tatcagtggg
     3121 ggttctgggt aattccatag attttgttat aaagaaaata attcctcgaa caatttccaa
     3181 aattgagagg tctaccaagg ttaatattgt tgcggcagca agtatcgaca agaagcctag
     3241 ggtcacctat gacgaaatcg gggggctgaa ggaacaaatc gataggctta gagagatcgt
     3301 cgagctgccg ctgcggcacc ccgaggtatt tgctaagcta ggaatagagc cccatagcgg
     3361 gatactcatg tacggatccc caggatgcgg aaagacattg atcgcaaaag ccttagcctc
     3421 agagtcagct gccaactttt atatcataaa tggtcctgag attgtaaaca aatattacgg
     3481 agaaacagaa gctcgtctga gagacatttt caaggaagca aaggaaacag caccaagtat
     3541 tatctttata gatgaaatcg atgccatagc accaaagaga gaagaggcat ttggggacgt
     3601 ggagaaaaga gttgttgctc agctgctggc tctaatggat ggaatgtcag accgtggcaa
     3661 tgtaatcgtg ttaggagcga cgaacagacc tgaaagtatt gatcctgctt tgagaagacc
     3721 gggtcggttc gatagagaga ttgagattgg agttcctaac gctcaaggac gattcgagat
     3781 actaggtatt cacacaagag gcatgccctt atctcaagac accgatctcc aggagttggc
     3841 ttcagaacta aatggttata ctggagcaga catcaaagct ttgtgtaggg aagcagccat
     3901 gaaggcacta aagcgctata ttccagagat cgactccgga agcggcgaag ttcctcctga
     3961 caccctgaga cttatggata tcagaagcaa ggactttaga gaaggaatga aggaaattat
     4021 tcctactgct atgcgagagt tttacgtaga ggtctcacga ataaaatggg aagacgtagg
     4081 ggggctgtac aatgccaaga gggtattgca cgataatttg ataatgtcca tcaaagagcc
     4141 tgatagtttt tccagaatgg gagtgagggc cccacgaggt gccctattat ttggacctcc
     4201 tggttgcgga aaaacattga tcgccaaatc gcttgcaacg ataagctctg ctaatataat
     4261 agttgtccgc
//
LOCUS       DASQTF010000040         6817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_932338,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000040 DASQTF010000000
VERSION     DASQTF010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932338"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1012
                     /gene="kae1"
                     /locus_tag="VE308_00980"
     CDS             20..1012
                     /gene="kae1"
                     /locus_tag="VE308_00980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex N(6)-L-threonylcarbamoyladenine
                     synthase Kae1"
                     /protein_id="HZA99573.1"
                     /translation="MLCLGIESTAHTFGCSIVDSRGKILSDARDVYKAPEGSGIHPRE
                     ASRHHMQVSPDTLQNALKSGNVTMKDIDIVGYSAGPGLGPCLRVGAVISRTVAGFYKK
                     PLVPVNHALGHIELGAMLTGASDPLVLLVSGGHTMILAFSHGRWRVFGETLDITVGQL
                     LDQFGRALGFASPCGNRIEQLASQSAGKYLQLPYIIKGNDVSFSGLLTAAVKLASCNT
                     ELADTCYSLQETAFAMLAEAVERALSFTSKKEMMIVGGVAANKRLAQMLDSVCNRQNA
                     KLFVCPIKFAGDNGAQIAWTALREYLATKMNVHVEESTVQQSWRLDTVNVSWRR"
     gene            complement(996..1319)
                     /locus_tag="VE308_00985"
     CDS             complement(996..1319)
                     /locus_tag="VE308_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99574.1"
                     /translation="MSFHPAPGEKILLDEDCSEDKLKSGILVLTDHRLVFQRTQGRIA
                     TLSKKETEMVLDIPLDKISSVRSEGFLVKKLVVIVAGDQIYKFGVFNNGKWERQIKQI
                     TSGAS"
     gene            1365..1967
                     /locus_tag="VE308_00990"
     CDS             1365..1967
                     /locus_tag="VE308_00990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF011463.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HZA99575.1"
                     /translation="MLIKRGAEADIYIVEWNSKKAISKIRSPKSYRHPDLDSAIRKYR
                     TMHEASFMSAAKSAGVMTPFVYFVDPVKAEIIMEFIEGQNIRDTLTPDICYKIGRCAA
                     LLHASNIIHGDLTTSNFVMNKRLILLDFGLSYYSERTEDMATDSRLIKEVFTSAHIAV
                     RKAFPSFVEGYASVAGRKKTDKILENVRTIERRGRYARMT"
     gene            1971..2522
                     /locus_tag="VE308_00995"
     CDS             1971..2522
                     /locus_tag="VE308_00995"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HZA99576.1"
                     /translation="METITFASINQNKFFEVQSILLTRNIIIDFSKIHLVEIQSDSLE
                     EIAQEKAKTAFAKVGRQVIVEDDGLFIDSLSGFPGQYSSFVFKTIGNDGILKLLEGST
                     KRSAYFRSVIAFYNGVTLSISDGRVDGRITDRITEDGGWGYDPIFVPDGTDLSFAELK
                     KSKNEYSHRKRALEKFAQWYLKL"
     gene            2828..2992
                     /locus_tag="VE308_01000"
     CDS             2828..2992
                     /locus_tag="VE308_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99577.1"
                     /translation="MTTAFLIGGENLRARGLRDDGRHDQDFVIPNDKDFEVMVSTLTD
                     IGFQHTTDGL"
     gene            3113..3697
                     /locus_tag="VE308_01005"
     CDS             3113..3697
                     /locus_tag="VE308_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99578.1"
                     /translation="MVDRAAYRRYGKLKLGLLHVNDIFLLKAVTDRQKDMQDILKIVK
                     TKDFDWGTVWEEMKLQEKDTREYISGFLLDTIDDLITEGVPAPAFLNRLLIKVVDTEI
                     KRYLREGEKPLEDLLGILLKHSSIKKEMVINRIEFLRKHKHLGTISRRDGIILKLRDR
                     GSLNIYRKYGASNSYQSALSLVDTIANKQDSQRR"
     gene            3718..3885
                     /locus_tag="VE308_01010"
     CDS             3718..3885
                     /locus_tag="VE308_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99579.1"
                     /translation="MHQLTEHSMFGGHKPQATVAAVLCVAAEEIGQHWSFTNTDVRTA
                     ANVSPPSFYHK"
     gene            complement(4325..5332)
                     /gene="twy1"
                     /locus_tag="VE308_01015"
     CDS             complement(4325..5332)
                     /gene="twy1"
                     /locus_tag="VE308_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HZA99580.1"
                     /translation="MQDNLIQITPQLKARLKKAKYGVYNHSAVELCHWTKKSFADGGN
                     CYKHKFYGISTHQCMEMTPTAMNCENRCIYCWRPTEFYDTLQMPEELVDEPDIIVEQL
                     MAERKKLINGFYGDPRNNKKKLDESLLPAHYAISLSGEPTMYPKLPRLIKYLRSLKAT
                     KSIFLVTNGQEPDMLRRLAVEDALPTQIYLSTNASNKKMFYQINRPRHRDAWERWWES
                     LRLLATLNTRTVLRMTLIKGYNDSYDFVSEFSDMMVQGNPHFIELKSYMHIGMSTQRL
                     EQDHMLEMNEIRNFAADLCAKMRSFKIMDESEISRIVVLQNEVRYVDRWIKGYCQQNI
                     P"
     gene            5426..6244
                     /locus_tag="VE308_01020"
     CDS             5426..6244
                     /locus_tag="VE308_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99581.1"
                     /translation="MAVDTDKIMKVGLELAGWKKMPADSAVHIKGKNISKVLIGIDIG
                     TAELILAKQLGCDAVIAHHPIGATAVNFYKVFDRHIDYMVEHGVPKKIARETVEKLKE
                     RIETRNHANIYSDVVGAAKALGMPLVNIHQPCDEYMRQVILRAIKSGRTEYVSDIVKS
                     ISKIPEFRHAATKIEVKFGKQKNKAGHWTLVVAAGTNGGFNIAKAYFQHGVSTVIYLH
                     IDYGELIKMREEKLEGNLVVMGHLGGDSIGLNGLAERLENLGIETVRVGILPNR"
     gene            6294..6391
                     /locus_tag="VE308_01025"
     tRNA            join(6294..6332,6357..6391)
                     /locus_tag="VE308_01025"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6329..6331,aa:His,seq:gtg)
     gene            complement(6468..>6817)
                     /locus_tag="VE308_01030"
     CDS             complement(6468..>6817)
                     /locus_tag="VE308_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HZA99582.1"
                     /translation="ENQTLGITVNSMSNGTLALRLPREVIDSQTAEGEDADFAAFIDN
                     AEFVEPGEIEPAEDTRTLLIGFPTGTGSIDVIGTSVIPEFSTIAVVVLAVAIVGIIIA
                     TARYGRFNIGPRM"
BASE COUNT         1964 a         1457 c         1546 g         1850 t
ORIGIN      
        1 gggatgatag ataataacaa tgctgtgcct tggaattgaa agtactgcgc atacatttgg
       61 ttgctccata gttgattcaa gaggcaagat actctctgat gcccgcgatg tctacaaggc
      121 accagaggga agcggtatac atccacgcga agcatcgcgg caccacatgc aagtgtcgcc
      181 agatacttta caaaacgctc taaaatcagg caatgtcaca atgaaggaca tcgacattgt
      241 cggatactca gctggaccgg gtcttgggcc atgtttgcgc gttggtgcag tgatatcaag
      301 gaccgttgca ggattttata aaaaaccgct cgtgcctgtc aaccatgccc ttggtcatat
      361 tgagcttggg gcaatgctca caggcgcttc cgaccctctt gtgctgctgg tgtcaggcgg
      421 ccatacgatg atacttgcat tttcacatgg ccgctggcgc gtttttggcg agacacttga
      481 catcaccgtc ggacaattgc tcgaccaatt tggtcgagct cttggctttg cctccccctg
      541 tggcaacagg attgaacagt tagcaagtca gtcggctgga aaatacttgc agctgccgta
      601 tatcattaaa ggaaacgacg tctcattttc aggcctgctg actgcagcag tcaagcttgc
      661 ttcatgcaat actgagttgg cagatacatg ctactctctg caggaaacag catttgcaat
      721 gttggcagag gctgtcgagc gggccctttc attcaccagc aagaaagaaa tgatgattgt
      781 gggtggagta gcagcaaata agaggcttgc tcagatgctt gacagtgtat gcaaccggca
      841 aaatgccaaa ttgtttgtct gtccaatcaa gtttgccggc gacaatggtg ctcagatcgc
      901 gtggactgca ttgcgggagt acttggctac aaaaatgaat gttcatgtag aagagtcgac
      961 cgtgcagcag tcgtggcgac ttgacactgt aaatgtcagc tggcgccgct agtaatctgc
     1021 tttatctggc gctcccactt gccgttgtta aatacgccaa acttgtagat ctgatctcct
     1081 gctacaatta ccaccagctt tttcacaagg aacccttcag acctgacgga agatatctta
     1141 tcaagcggta tgtcaagaac catctcagtc tccttttttg aaagagttgc tattctgcct
     1201 tgtgtccttt gaaataccaa cctatggtca gtcagcacga gtataccgct tttcagcttg
     1261 tcctcggagc agtcttcatc aaggagtatt ttttcgcccg gtgctgggtg gaaactcacc
     1321 acatttttat cttcaaagca gagcctatat tactattgca gcccttgctg ataaaaaggg
     1381 gagcagaagc cgatatctac attgtagagt ggaatagcaa gaaggcgatt tctaaaattc
     1441 gctcacctaa gtcctaccgc catccagatc tagactccgc aatccgaaag tacaggacta
     1501 tgcatgaagc aagttttatg tctgctgcca agtccgcagg cgtcatgaca ccattcgtgt
     1561 atttcgtcga ccctgttaag gctgaaataa taatggagtt tattgaaggg caaaacatcc
     1621 gagacacgct aacgccagac atctgctaca agataggtcg ctgtgccgca ctgctgcacg
     1681 caagcaacat tatccatggc gacctgacga cttcaaattt tgtaatgaac aaaaggctta
     1741 tcctcctaga ctttggcctt tcatactatt cagagcgaac cgaagacatg gcaaccgata
     1801 gccgacttat aaaagaagta tttaccagtg cacacatagc agttagaaaa gcctttccta
     1861 gctttgtaga aggttatgct agtgttgctg gcagaaaaaa gacagataag attctagaaa
     1921 atgtcaggac tatagaacgg cgcggaagat atgcgaggat gacttagaga atggaaacta
     1981 taacttttgc aagcattaat caaaacaaat tcttcgaagt ccaatcaata cttttgaccc
     2041 gaaacattat catcgacttt tcgaaaatcc atcttgttga aatccagtca gactcccttg
     2101 aggaaatagc acaggagaag gcaaaaactg catttgcaaa agtggggagg caagtcatcg
     2161 tcgaagacga cggcctgttt atcgatagtc tttcaggatt tccgggccaa tattcttcat
     2221 ttgtattcaa aaccattgga aatgatggta tactgaaatt acttgaaggc tcaaccaagc
     2281 gatcagcata cttccgctcg gtgattgcct tctataatgg agtaacatta tcaatatctg
     2341 atggaagagt tgacggcagg ataactgaca ggataacaga ggacggtggt tggggttatg
     2401 atcctatatt tgttcctgat ggcaccgacc tctcatttgc agagctaaag aaaagcaaga
     2461 atgaatattc gcaccgcaaa agagcacttg aaaaatttgc acagtggtac ctaaagcttt
     2521 gacagtgtaa tcctgtattc tttggaataa gggaaatgaa aggatccaga tattctcaat
     2581 cgcttagtga taagaggatg ttatctactt tggtctgata ttctcaaagt ttgctcagct
     2641 aaattactgt tatcgtgcac atgattaagc tgatctgtct tttgtgtcca agattgctgt
     2701 cctagcagat gacttatagt atattaatgc agagcttatg taacagacat agatggaccc
     2761 aatgccttta gccattggag catccaatac tgcattatac aagcaaatag gctcaagatt
     2821 gcctaaaatg acaacagcgt ttttgatagg aggcgaaaat ttgcgtgcga ggggtttgag
     2881 agatgatggc aggcatgacc aggattttgt aattccaaat gataaagatt ttgaggtaat
     2941 ggtatctaca ctgactgaca taggctttca acatactact gatgggctat agtcaaaaag
     3001 tcgcagtgat tccaacatgc ggtcaagaga aatactgcag catccaaagt ttggactagt
     3061 tgatgttttt aacattatca ttggtaaaaa tcgctgctac ttgtcgaaaa ggatggtaga
     3121 ccgagcagcc tatagaagat atggcaagtt gaagcttgga ttattgcatg tcaatgacat
     3181 ctttctccta aaagctgtca cagacaggca aaaagatatg caagacatct tgaaaattgt
     3241 aaaaacaaag gactttgatt ggggtacagt ttgggaggaa atgaaactac aggagaagga
     3301 tactcgtgag tacatttcag gctttttact ggataccatt gatgacctga taacggaagg
     3361 agtacctgcg cctgcatttc tgaacagatt gttgataaaa gtcgttgaca cagagatcaa
     3421 gcgatacctg cgtgaaggcg agaagccact tgaagatttg ctcggcatct tgctcaagca
     3481 cagtagcatt aagaaagaga tggtcataaa tcgcatagag tttcttcgca agcacaaaca
     3541 tttaggaaca atatcaaggc gtgacggcat tatactgaaa ctcagagacc ggggcagcct
     3601 caatatctac aggaaatatg gagcatctaa ctcctatcag agtgctcttt cacttgtcga
     3661 tacgatagct aataaacagg actctcagag aaggtaaaac aaaaagccat tgaaattatg
     3721 catcagttaa ccgagcatag tatgtttggt gggcacaaac cacaggctac tgtcgcagca
     3781 gtgctctgtg ttgcagcaga agagataggc cagcattggt ctttcaccaa taccgatgtt
     3841 cgtacggctg caaatgtcag tccacccagc ttttatcata aataaaggcg aaaatggctt
     3901 ttcgttcaag tatccaaatg agcaaatgac tgctatccgc agacgccgca gattaaaggt
     3961 aaacaatttt cgatttgtaa gatatgtgta acacttcctg gagctagagt aaacgccatc
     4021 gaacttctaa tcatatatgc aataaatgcg ttgatatgtg gaccaatttg aacatcagat
     4081 ggaacgcgca gcctaagcag caagtgcaat gatatccttc ggaatacact ccaaaggcta
     4141 ctcgataact gaatctgacc attcctaaat aatgataata caggttaaag caaaagtcca
     4201 tatttcatac tgttatttaa taaccaaaac acaaccaatg tggaatctag aatttttgct
     4261 tattttggtg ttaggaagct gctaataata cagatcgatg aaatgctgcc cccttcacaa
     4321 gtgtctaggg gatattttgt tgacaatatc ccttaatcca cctgtcgaca tagcgcacct
     4381 cattctgtag taccactatc cttgaaatct cgctttcgtc cattatcttg aacgaacgca
     4441 tcttggcaca caagtctgct gcaaagttcc ttatttcatt catttccaac atatggtctt
     4501 gctctaatcg ttgggtcgac atgccaatat gcatataaga tttcagctca ataaagtgag
     4561 ggttaccctg caccatcata tctgaaaatt cactaacaaa atcgtaactg tcgttgtatc
     4621 ccttgatcag agtcatccga agcacagttc tggtgttgag ggttgcaagt aacctcaggc
     4681 tctcccacca gcgttcccat gcgtcgcggt gacgtggacg gtttatctga tagaacattt
     4741 tcttgtttga cgcgttagtc gataggtaaa tctgagtggg cagtgcgtct tctacagcta
     4801 acctccttag catatccggc tcttgtccgt ttgtcactag aaaaattgac ttggttgcct
     4861 tgagtgacct taagtatttt atcagccgcg gcagcttagg gtacatcgtg ggttcgcctg
     4921 ataacgaaat tgcataatgg gctggcagca gcgattcgtc aagttttttc ttattattcc
     4981 tagggtcgcc ataaaagcca tttatcagct ttttccgctc agccatcaat tgttcaacga
     5041 taatatcagg ctcgtcaact agctcctctg gcatctgcag tgtatcataa aactctgttg
     5101 ggcgccaaca ataaatgcac ctattttcac agttcattgc agttggagtc atctccatgc
     5161 attggtgagt ggagatgccg tagaacttgt gtttatagca gttgcccccg tccgcaaacg
     5221 acttttttgt ccagtggcac agctctactg cagagtggtt gtagacccca tacttagcct
     5281 tctttagcct tgcttttagc tgtggtgtga tctggatcaa gttgtcctgc atagagtgtg
     5341 aaacgtgctc gtctgagcag ctcatgctcg ctttgtatac ggttgtagat cagatttaaa
     5401 tttttcactg tgcggactgg tagccgtggc tgtagatact gataaaataa tgaaagtggg
     5461 gcttgagctt gccggctgga agaaaatgcc ggcggacagc gccgtgcaca taaaaggtaa
     5521 gaacataagc aaagtgctga tcggaataga cataggaacg gcagagctga tccttgcaaa
     5581 gcaacttggg tgtgacgcag tcattgctca ccaccccatt ggtgctactg cagtcaactt
     5641 ttacaaggta tttgataggc acatcgacta tatggtagag catggtgttc caaagaaaat
     5701 cgcaagagag accgttgaga agctcaagga gcgaatcgag acacgcaacc atgctaatat
     5761 ttatagcgat gttgtcggag ctgccaaggc acttggcatg ccacttgtca atatccacca
     5821 accatgtgac gaatacatgc ggcaggttat attacgcgcc atcaagtctg gcaggacaga
     5881 atacgtatca gatattgtca aatctattag caagatccca gaattccgtc atgctgcaac
     5941 caagatagaa gtaaaatttg ggaaacaaaa aaacaaagca ggccattgga ctttagtggt
     6001 ggccgcaggc accaatggtg gctttaacat tgctaaggca tactttcagc atggtgtttc
     6061 tacagtcatc tatctgcata tagattatgg tgaactgata aaaatgaggg aagaaaagct
     6121 ggagggcaac cttgtagtaa tgggacactt agggggtgac tcaataggcc tcaacggcct
     6181 tgcagaacgg cttgagaacc taggcattga aactgtcaga gtcggcatcc ttccaaatag
     6241 atgatggtcg aacgattata tactgtagtt gttgcaaatt ttctggctgg ctggccttcg
     6301 tagtatagcc tggttagtat aggcggctgt ggtcaatttc ttaacttccg gcagagaccg
     6361 cttgaggagg gttcaaatcc ctccgaaggc ccctcttacg atgatgaata caatacacac
     6421 ctttcggtaa gacttttcaa ctaataaaaa gaaggaggaa aaaagtttta catccttggg
     6481 ccaatattga acctgccata tcttgcagtt gctatgatga ttccaacaat tgcaacagcc
     6541 agcacgacta cagctattgt gctgaattct gggattacag atgtacctat aacatcgatc
     6601 gaccctgtgc ctgttggaaa gccgattagc agcgttctgg tgtcttctgc tggttcgatt
     6661 tcgccaggtt ctacaaattc agcgttatca ataaatgccg caaaatccgc atcttctcct
     6721 tcagctgttt gagagtcaat tacctctctt ggaagtctga gagctagcgt tccattactc
     6781 attgagttaa ctgtaattcc aagcgtttgg ttctcgc
//
LOCUS       DASQTF010000041         6142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1302791,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000041 DASQTF010000000
VERSION     DASQTF010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302791"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..2006)
                     /locus_tag="VE308_01035"
     CDS             complement(435..2006)
                     /locus_tag="VE308_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZA99583.1"
                     /translation="MFSNEHTLHRFMMEKAEGHFHDRYEKALEQVGSEFGRTYTLLIG
                     GSEITTADTISHTSPIDTRIVLGHMPIGGASHVRKAIAAAKEAFERWGRIDYRERVHV
                     CKVAADIMADRKFELAAWVSYENGKNRYEAIADIDEAVDFLRYYSEEMERNNGFETMM
                     KSAQTNEKSKSVMKPYGIWGVIAPFNFPAAIFVGMSTGAIITGNTVVAKPSSNTPIIA
                     WKFAKIFKQAGLPDGVFNLVIGPGSKIGNELVSNKDVAGIVFTGSRDTGFRMAKEFSK
                     TRLKPLIAELGGKNPVIVTETADINNAVDGVLKATFGYSGQKCSACSRVYIHKKVKDE
                     FLRRLVEKTKNLPIGNPLDSNTFVGPLANEDAYRKYQKYIRTAEKDGKVLVGGSIKKD
                     DDLKYGYYVEPTIIVGLPKKHRLFREEMFVPILCAADYEKFDDALKLANDTEYGLTAG
                     IFTRKQEEVKKFLDCIEAGVVYVNRQASATTGAMVGCQPFGGWKESGTRGKGTGGPHY
                     LTEFMHEQSQTIAVE"
     gene            complement(2317..2988)
                     /locus_tag="VE308_01040"
     CDS             complement(2317..2988)
                     /locus_tag="VE308_01040"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HZA99584.1"
                     /translation="MKVIINSAMTVDGKIATSSGDSKISSKEDLVRVHKLRASVDAIV
                     VGISTILADNPRLTVRLAKGKNPARVIVDSRGRIPPDSQIMRTASKVRTIVAVTDQAP
                     EEMIMKLRDMGAQVLVISEGKKGQSAAVPRGVNLKMLFRKLEKMGLKKILVEGGGELN
                     WSLLHLWLVDELTITIAPKIAGGRLATTLVEGDGFDEIAQSIKLHLKKIERKKGGELV
                     LYYKL"
     gene            complement(2985..4169)
                     /locus_tag="VE308_01045"
     CDS             complement(2985..4169)
                     /locus_tag="VE308_01045"
                     /inference="COORDINATES: protein motif:HMM:NF005552.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZA99585.1"
                     /translation="MSLAITNASLLLGRDLEYVEQGYIEIENGKIRSTSAGSYKGSNK
                     KLDAKGFIVIPGFINAHTHIADSIGKDIAAGQRLDARVHPVFGAKKKILQKSLPEHLK
                     TFIRNSAILMMKKGIVAFADFREDGLDGIRLLKDAVDGLPIKCVTLGRINFYSSPTDA
                     AGLPPEKGEQAKQVIELADGLGISGANENTDAALAQYRQLAGKKLIAIHAAESKETVK
                     FAKVHTGRSEVDRVMEYLKPDFVVHMTNATENEISLVGKSGTGIVICPRANGVLGAGI
                     PKVAQMLRQDCLVAIGTDNVMLNSPDILRELDYTWKASRATEGKMLEAQELLKMATIN
                     AAQILRLNTGCIEVGRAADLVFIDKKHADLYPMHDPYAAIVHRLSQSSIGAVMIDGRF
                     VD"
     gene            complement(4160..4912)
                     /locus_tag="VE308_01050"
     CDS             complement(4160..4912)
                     /locus_tag="VE308_01050"
                     /EC_number="3.5.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase IIa"
                     /protein_id="HZA99586.1"
                     /translation="MIQLTIIRIEGYGKWTLTLGSDREAQLQMLQARIYRDVQRLFSE
                     RNSIVFFNRFDEYFAITNGLGADDHLHIQHELTKLYDNMSMSMTIGAGKTAFEANLNA
                     YRTRKEGRMLDSNARIFGSVHPQADIAQIMHIDIDSSAKVGNRLSPYELTVLVMKIYS
                     RLAEEFLKHDSLAFFLGGDNVMVISNGMAKEQADEVLKKVTAGNDIKLNCGIGIGKTG
                     RRAAEAATKALDTIRDLREEGKIQTIYEIQCP"
     gene            complement(4890..5381)
                     /gene="ribH"
                     /locus_tag="VE308_01055"
     CDS             complement(4890..5381)
                     /gene="ribH"
                     /locus_tag="VE308_01055"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZA99587.1"
                     /translation="MPGWHNQQLIYLAIVVSEFNREITFRMLQDARDHAANVSAEISY
                     ILYVPGTFDMPLAVERLLRKKKVDAVVTLGAVIKGDTRHDDIVAENAARLIADLSLKY
                     GKPVTLGVAGPGMTIEQARARAKLVPTRAVNAAVNMVLRLRKLQKAVNGGIVIINDTA
                     DNN"
     gene            complement(5365..6045)
                     /gene="ribB"
                     /locus_tag="VE308_01060"
     CDS             complement(5365..6045)
                     /gene="ribB"
                     /locus_tag="VE308_01060"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZA99588.1"
                     /translation="MSLAEAIAALKAGRFVLVHDDKARENEIDMVIAAEHIQPYHIAT
                     MRKDAGGLVCLAIANEITSKLGLVYMHDIIADMGKVNPVFSKLTEGRTTYGDKPSFSI
                     AVNHRSTYTGITDQDRALTILKMAEVCKNIENGGVEQFARNFHAPGHVPILIASKHLL
                     HDRMGHTELCVYLTQLADITPAVAICEMMDSTTHRALSIDAGQKYAAKFGMPLIDASE
                     LKANARVA"
BASE COUNT         1444 a         1551 c         1240 g         1907 t
ORIGIN      
        1 aaagcattgc tagtatgcat gatgggcaat acttcactca aagttgctga agtacttgca
       61 gaaaaaggca ttgtagctga aagcttgact gggggaattt cagcactttc tcaagataaa
      121 ggtaagcaaa tttctgaatt ggtgcgaata gcggcagaat aataggcatt ccaagagaga
      181 tagaagcggc cctcttttca atttgggcgg attacagcag atagtattca atgactttga
      241 aatctgcagt aggcaaacag ttgctacatt attttttcga agcataatat tgctctagtc
      301 acttcgaaca gtacttctgt acttcgctat atcaaacatt tgacatgaat cctagctcct
      361 tctttatgct cattgatgca ggtctagtct ctaatggttt gaccacggat agaatatgat
      421 gaaatattta tcgactattc aacagcaata gtctgactct gctcatgcat gaattctgtc
      481 aggtaatgag gtcctcccgt tcctttaccc ctagtaccgg actccttcca tcctccaaat
      541 ggctggcatc cgaccattgc gccagttgtt gcgcttgcct gcctgttaac ataaacaact
      601 cccgcctcga tgcaatcaag gaactttttg acctcttcct gcttcctggt aaatattccg
      661 gccgtcaatc catattcagt gtcgtttgca agcttgagag cgtcatcaaa cttctcatag
      721 tcagcagcac ataggatggg aacaaacatc tcttctctaa agagccggtg tttttttggc
      781 aggccaacga taattgttgg ctctacgtag tagccgtact ttaagtcgtc atcttttttg
      841 atagatcctc caacaagcac cttgccatcc ttctccgctg tccttatgta tttttgatat
      901 ttgcggtagg catcttcgtt ggcaagtgga ccaacaaagg tattggagtc aagtgggttt
      961 cctattggta gattctttgt cttttcaaca agccgcctga gaaactcgtc cttcaccttc
     1021 ttgtggatat aaacacgaga gcaggcgctg catttttgtc ccgaatagcc aaacgttgct
     1081 ttgaggacac cgtcaactgc gttattgatg tcagccgttt cagtcactat caccgggttc
     1141 ttgcctccaa gctcagcgat tagcggcttg agtcttgtct tgctaaactc ttttgccata
     1201 cggaagccgg tgtctcgaga accggtgaat acaatcccag caacatcctt gttgctgaca
     1261 agctcattgc ctatcttgct gccgggtccg atgactaggt tgaacacacc atcaggaagt
     1321 ccggcctgct tgaatatttt tgcaaacttc catgcaataa ttggagtatt gcttgacggc
     1381 ttggctacca cagtattccc ggtgattatt gcaccggtgc tcatgcctac aaaaatggca
     1441 gcaggaaagt taaagggagc aattactccc catattccat agggtttcat gacgctcttg
     1501 ctcttttcgt ttgtctgagc gcttttcatc attgtttcaa aaccgttatt tctttccatt
     1561 tcttcagaat agtagcgtaa aaagtcgaca gcctcatcga tgtctgctat agcttcgtaa
     1621 cgattcttgc cgttttcata tgagacccac gcagcaagct caaacttgcg atcagccatg
     1681 atgtctgctg caaccttgca tacatgtact cgctcccggt agtctattct gccccagcgc
     1741 tcaaatgcct cttttgcagc tgcaatggct ttcctgacat ggctggcacc cccgatcggc
     1801 atatgtccaa gcactattct tgtgtcaatt ggcgaggtat gcgatatcgt atctgcggtc
     1861 gttatctcac ttccgccaat cagcaatgtg taagtcctac caaactctga tcctacctgc
     1921 tcaagtgctt tctcgtacct gtcatgaaaa tgtccttcag ccttttccat catgaatctg
     1981 tgcagagtgt gctcgtttga gaacatctac actaatatgg ttgaaagaac cttaaaaatt
     2041 atgagcacaa aaacaagcga atgctgatta cttttgcgca tcgtgctaca ttgaatagtc
     2101 tatctcatct tcagccagat tcttccattt tgctttacat agcatagtct tgctagagtt
     2161 actggctata aggagttcga gtacttctca gccgggaatc acgaacctca atttcatgcc
     2221 gtcgatcaca ttcgaattga atttttggca aggctgtcaa gcccagatac tgtttctgtt
     2281 gcattatgag caagatgtaa aaagattgct atacaatcat agtttgtagt acagtacaag
     2341 ctctcccccc tttttccgct ctattttctt gagatggagc ttaatgcttt gggctatttc
     2401 atcaaatcca tctccctcta caagcgtcgt tgccagccta ccgcctgcaa ttttcggggc
     2461 aattgttatt gtcagctcat cgacaagcca aaggtgcaaa agagaccagt taagctctcc
     2521 tccaccctcc accagtattt ttttcagccc catcttttcc agcttgcgaa acagcatttt
     2581 aagattgacg ccgcggggca cagcggcact ttgtcccttt ttcccttcgc ttatgaccag
     2641 cacctgcgcg cccatatctc ttaatttcat tatcatttcc tcgggtgcct gatctgtcac
     2701 tgctacaata gtcctaacct tggatgcagt acgcattatt tgagagtcag gtgggatcct
     2761 gcctctgctg tcaactatca ccctagcggg gttcttaccc ttcgctaatc ttactgtgag
     2821 ccgcgggttg tctgccaaaa ttgtagaaat tcccactact atggcatcga cagaagcacg
     2881 cagcttgtga accctcacga gatcttcctt agaagaaatt ttcgaatctc cgctggatgt
     2941 ggcgatcttg ccatcaactg tcattgcact gttgattatt accttcaatc tacaaaccgc
     3001 ccatcgatca tgactgctcc gatagagctt tgactcagcc tgtgaacgat tgcggcatat
     3061 ggatcatgca ttggatagag gtcggcatgt tttttgtcaa taaatactag atctgcagcg
     3121 cggccgacct ctatgcatcc agtattgagt cttaaaattt gcgccgcatt tatagttgcc
     3181 atcttcaaga gttcttgtgc ttctaacatc ttaccttcag tcgcccttga cgccttccag
     3241 gtataatcca gttctctcaa aatatctgga gaatttagca taacgttgtc cgttccgatg
     3301 gctacaaggc agtcttgcct gagcatctgg gcaacctttg ggatgcctgc gccgagcaca
     3361 ccatttgccc gagggcaaat tacgataccc gtcccacttt ttccaacaag cgatatttcg
     3421 ttttctgttg cattggtcat atgtacaaca aagtcaggct tcagatattc cattaccctg
     3481 tctacctcac ttctgccggt gtgcacctta gcaaatttga cagtctcttt tgactcagcg
     3541 gcatgtattg cgatcaattt ctttcctgcc agctgtctgt attgtgcaag agcagcatca
     3601 gtattttcat ttgcaccgct tattccaagt ccatctgcca gctcaattac ctgtttagcc
     3661 tgctctcctt tttcaggcgg caggccggcc gcatctgttg gactgctgta aaaatttatc
     3721 ctcccaagcg ttacgcattt tatcggcaga ccatcaactg catcttttag gagccttatc
     3781 ccatccagtc catcttctcg aaaatcggca aatgcaacta ttcccttttt catcattaaa
     3841 attgcggaat tcctgatgaa ggtctttaga tgctcgggta ggctcttttg cagaatcttc
     3901 ttcttagcac caaacacagg atgcactcgt gcatcaagcc gctgcccagc ggcaatatct
     3961 tttcctattg aatcggcaat gtgcgtgtgc gcgttgataa atcctggtat gactatgaat
     4021 ccctttgcat ccagcttctt gttggaaccc ttgtaactac cagcagaggt gctccttatc
     4081 ttgccatttt caatctcaat gtagccttgc tcaacatatt caagatctct gcccaaaaga
     4141 aggctggcgt ttgttattgc tagggacatt gtatttcata tattgtttgg atttttcctt
     4201 cctcgcgcag gtctctaatt gtgtcaagtg cctttgtggc ggcttctgcc gctcttctgc
     4261 cggtcttgcc aatccctata ccgcagttga gcttgatatc attgccagcc gttaccttct
     4321 ttaggacttc gtcagcctgc tcttttgcca tgccattaga aattaccata acgttgtcgc
     4381 cgccaagaaa gaacgcaagc gagtcgtgct tcaggaactc ttctgcaagc ctgctgtaga
     4441 tcttcatgac gagtactgtc agttcataag gcgacagccg attgccaact tttgcagagc
     4501 tatcaatatc gatatgcatt atttgggcta tgtctgcctg agggtgtaca ctgccaaata
     4561 ttctagcgtt actgtcaagc atcctcccct cttttcttgt acggtaggcg ttcaggtttg
     4621 cttcaaatgc agtcttgccg gcgccaattg tcatcgacat tgacatgttg tcatacagct
     4681 ttgtaagctc atgttgtatg tgtagatgat catcagcacc aagaccattt gttatcgcaa
     4741 aatattcatc gaacctgttg aagaaaacaa tgctatttct ttccgagaat agcctttgta
     4801 catcacgata gatccttgct tgcagcatct gaagctgtgc ttccctgtca ctcccaaggg
     4861 tgagagtcca tttcccgtat ccttcaatcc taattattgt cagctgtatc atttattatc
     4921 acaatccctc cgttgactgc cttttgtagc tttctcaacc gcaaaaccat gtttacagcc
     4981 gcgttcactg ctctagtcgg cacaagcttt gctcgcgctc ttgcttgctc gatcgtcatt
     5041 ccaggccctg ctactccaag tgtcaccggc ttgccatact ttagcgatag atcagctatc
     5101 agtcgtgcgg cattttcagc tacgatgtca tcgtgccggg tgtctccttt tatcaccgcg
     5161 ccaagcgtga ctacagcgtc tacttttttc tttctcaaca atcgctcaac tgctagcggc
     5221 atatcaaagg tgccaggcac atacaagatg taacttatct ctgcacttac attggctgcg
     5281 tgatctctcg cgtcttgtag catcctgaac gttatctccc tgttaaactc ggaaactact
     5341 attgcaagat aaatcaattg ttgattatgc caccctggca ttagctttga gctcgcttgc
     5401 atcgattaaa ggcataccaa attttgcggc atatttttgc ccagcgtcaa ttgagagcgc
     5461 cctgtgagtc gttgagtcca tcatctcaca aatggctact gccggcgtaa tgtctgcaag
     5521 ctgtgtcaga taaacgcata gctcagtatg acccatcctg tcatggagta gatgctttga
     5581 tgctataagt atcggcacat ggcctggtgc atgaaagttc ctagcgaatt gttctacgcc
     5641 gccattttca atattcttgc atacttctgc catcttcaat atggtaagtg ccctgtcctg
     5701 atcggtgatg ccagtgtacg tagagcgatg gttgaccgct attgaaaatg acggcttatc
     5761 tccgtaggtg gttctaccct ctgtcaattt tgaaaatact gggtttacct tgcccatatc
     5821 tgctatgatg tcgtgcatat agaccagccc cagcttgctt gtgatctcgt ttgcgattgc
     5881 aaggcacaca agtcccccag cgtcttttct catcgtcgca atgtgatacg gctgtatatg
     5941 ctccgcagca attaccatat cgatctcatt ctcacgcgct ttgtcgtcat gaactagtac
     6001 aaacctgcct gccttgagag ctgctattgc ttcggcaaga gacaactagt aatttttgtc
     6061 gctcatctta ttaatagatt actaaaatct tggttattac caggcttttg gtagtgttgc
     6121 gttaatacgt gttttaaaag ga
//
LOCUS       DASQTF010000042         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_562302,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000042 DASQTF010000000
VERSION     DASQTF010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562302"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VE308_01065"
     CDS             <1..936
                     /locus_tag="VE308_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HZA99589.1"
                     /translation="STGPFMLAIVIVLGVSYAITYGVTQDPLMGIYGTAMAAMAMLSL
                     AGIVLCIDAFGPISDNAGGIVEMTNMGEENRTITDKIDSAGNTTKAITKGFAIASAGL
                     AALAIIQAFQLEADRIFPAMMFEYSLSDVRLIIGLLVGGLIPFFVTSQLIAGVSRSAS
                     KMVDEVRRQFKEKPGIMDRTETPDYAKCVDIATTASLRELWKPALVTIAAPIVIGVLL
                     GPTAVAGVLMGAVVSGLFLAYHLANSGGAWDNAKKYIEMQGKKKTEAHAIAVVGDLIG
                     DPYKDTAGPALNTVIKLLNTVAIVFVPVFIAVLPL"
     gene            1263..>4025
                     /gene="ileS"
                     /locus_tag="VE308_01070"
     CDS             1263..>4025
                     /gene="ileS"
                     /locus_tag="VE308_01070"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZA99590.1"
                     /translation="MHFNVNFNAKAIENNVRDYLGNLDLTAYLEAELAGKELIGYIEG
                     PPTMNGEPHVGHIRGRIIKDLWYRFNTLQKKRVVFRAGWDTQGLPVELQAEKELGLTG
                     SKAENVNKVGVEKIVETCKKIVHLYNERWVAVDKLLGMSFDYEKAYWTFHDQYIEREW
                     QYLKKAWENGVLKEWFRVVAYCPSCQTSLSNAEVNQGYETVEDPSFYYKVKLREEEDV
                     YLIVWTTMPFTIVTDEMVGANPKANYNYVRINDDELWIVGAERMKDLMKELHIEDFKV
                     EKTVKGSELDGRYYVHPLLHIIPSLEELAANKSIHFVVTDDFVDTATGSGLVHLSPAN
                     GEQDFEIATKRNVPIFVPIDDRVIFTEKAGDFKDLFVRDADNKVVDAMKEANASIKIG
                     KIKHQYPTCWRSHHKVVWLARREYFYIIEKLGQKPLHAAENVEYHFEPPKNRFIEIIR
                     EQHPWCISRERVWGTPLPIWSCSKCSYKDLLISRREIISKAIELPDGPDFELHRPWID
                     RIKIKCEKCGETMQREQFVLDTWHNSGAAPYASLTDQEYKDLIPATFLTEGIDQTRGW
                     AYTLLIENVIMKQSGIAPFRSFLFQGHVLDKKGNKMSKSLGNIIEADTLLTENPVDLI
                     RLYFMWKSSPIESLSFSVDEMKTRPYQILSTLHNLHVYFKQNSEFDRFDQEKHTLNWI
                     TDNNLLSSTEIWLLSKLQELVAEVTESFDQCRFHEAAKAVDAFVINQLSQTYVPITRN
                     IIWDDSPDNLDRRLAVYSVIGHALMQLNIMLHPLSPFITEYLYVTCFNRNKSILLESW
                     PKRDEKLIDSKVELAFDSIKEIVSLANAARNLASLKRRWPIKEVIICGQNLKSLDIEG
                     ISDVLKSQLNADQYRLVEIAAGSQLNKVVNLLDKKMPISVSISLVRKNVAPRVKADIN
                     RVVEAFD"
BASE COUNT         1238 a          845 c          980 g          962 t
ORIGIN      
        1 tcaactggac cctttatgct tgccatagtc attgtacttg gagtttcata tgctattaca
       61 tacggagtga cgcaggaccc gcttatggga atctatggta cagcgatggc tgcaatggcg
      121 atgctcagcc ttgcagggat cgttctttgt attgacgcat ttgggccaat aagcgacaat
      181 gctggcggta ttgttgaaat gactaacatg ggcgaagaga atcgaacaat aactgacaag
      241 atagactctg ctggcaatac caccaaggcc ataacaaagg gctttgcgat cgcaagcgcg
      301 ggccttgctg cacttgctat tatacaggca tttcagcttg aggctgacag gatattccca
      361 gcaatgatgt ttgaatactc gctttcagat gtgaggctca taattgggct cttggtgggg
      421 ggcctcatcc cattctttgt aacaagccag cttatcgcag gagtcagtag atcagcttca
      481 aagatggtcg acgaagtgag acgccagttt aaggaaaagc cagggataat ggaccgcacg
      541 gaaactccag actatgctaa atgcgtggac attgcaacga ctgcaagcct aagagagctg
      601 tggaagcctg cgctcgtcac aattgcggct ccaatcgtaa taggagtgct gttaggtcca
      661 actgctgtgg caggcgtgct catgggtgcc gtggtaagtg gcctattcct tgcataccat
      721 cttgcaaata gcggaggcgc atgggacaac gccaagaaat acatcgagat gcagggtaag
      781 aaaaagactg aggctcatgc aatcgcagtc gtaggcgacc tgattggaga cccatataag
      841 gacacagctg ggccagcgct caacacggta attaagttgc tcaacactgt agcaatagtg
      901 tttgtgccag tattcatcgc agtattgcca ctgtaatcag agttcaaaat ctgtgttggg
      961 tcctctcctg ctgagcgaag gaagacatgt agtcagtaca tcgcaaaagg atactagcgg
     1021 ccattattaa ctggattata taatactctt cgggatagta tagacaaacg ggcagagaga
     1081 ataccgcaca aaaagatcgg gcaaaaaacc aaaataatga aaaagatgga ggctacgagc
     1141 tctaatactt ttttgcatga attgtgtaga taagtaacca taattacgat taatcaaatt
     1201 gtgctctagc ttgaatagcc aaaaacataa acgcaggctc tgctatcgag attctcgcac
     1261 agatgcactt taatgtcaac ttcaacgcca aggctatcga aaacaacgtt cgagattatc
     1321 tgggtaatct tgatttaaca gcttacttag aagctgagct ggcaggcaaa gagcttatcg
     1381 gctacataga gggacctcct acaatgaatg gcgagcccca tgtgggtcac atccgaggca
     1441 gaattatcaa agatctttgg tatcggttta acactttaca aaaaaagaga gttgtctttc
     1501 gcgcaggctg ggacacacag ggcttgccgg tagagctgca ggcagaaaaa gagcttgggc
     1561 tgacaggcag caaggcagaa aacgtcaaca aggttggagt ggaaaaaatt gttgaaacct
     1621 gcaagaagat agtccatttg tacaacgaaa gatgggttgc agtcgacaag ctacttggca
     1681 tgtcgtttga ttatgaaaag gcatactgga ctttccatga tcaatacata gagcgagaat
     1741 ggcagtattt gaagaaggcg tgggaaaacg gagttctgaa agagtggttc agagtagtcg
     1801 cgtattgtcc atcatgccaa acatctctta gcaatgctga agtgaatcaa gggtatgaaa
     1861 ctgttgaaga tccttctttc tactataaag tcaagctgag ggaagaagaa gatgtatacc
     1921 tgatagtgtg gacaacaatg ccttttacaa tcgttacaga cgagatggtg ggagcaaacc
     1981 ctaaggctaa ttataattat gtgcgcatta acgatgacga gctgtggatt gtcggtgccg
     2041 aaagaatgaa ggaccttatg aaggaattgc atattgaaga cttcaaagtt gaaaaaacgg
     2101 tcaagggaag cgagcttgac gggcgatatt acgtccaccc tttgctccat atcataccaa
     2161 gcctagaaga gctggcagcc aacaaatcga ttcactttgt ggtcacggat gattttgttg
     2221 acacagccac aggcagcggt cttgtgcatc tctcaccagc aaacggcgag caagactttg
     2281 agatagcaac gaaaagaaat gttcctattt ttgtaccaat agacgataga gtaatcttta
     2341 cagaaaaagc tggggacttt aaggatctct ttgtcagaga tgccgataat aaagtggtgg
     2401 acgcaatgaa ggaagctaac gcatcaatca agatagggaa gataaagcac caatacccta
     2461 catgctggcg gtctcatcac aaagtagtgt ggctcgcccg gcgcgaatac ttctatatta
     2521 ttgaaaagtt ggggcagaag ccactgcatg ctgcagagaa tgtagagtac cattttgagc
     2581 cgccaaagaa ccgattcata gaaataatca gagagcagca cccttggtgt atatcaaggg
     2641 aacgtgtttg gggtacccct cttcccatat ggtcgtgcag caagtgttct tacaaagact
     2701 tgctcatctc aagaagagag atcataagta aggctatcga gttaccagac ggccccgact
     2761 ttgagcttca tcgcccctgg attgatagga tcaaaataaa atgcgaaaaa tgcggagaaa
     2821 cgatgcagcg cgagcaattt gtgctcgaca cgtggcacaa cagcggcgct gcgccctacg
     2881 cgtcacttac cgatcaggaa tacaaagatc tcattccagc cacctttctg acagagggca
     2941 tcgaccagac aagaggatgg gcctatactc tcttgataga aaatgtcatc atgaagcagt
     3001 caggtatcgc gccatttaga tcctttcttt ttcagggcca tgttctcgac aaaaagggca
     3061 acaagatgag taagagcctt ggaaacatca ttgaggctga cacgctcttg actgaaaatc
     3121 cagtagacct tattaggttg tactttatgt ggaagtcgtc ccccatagag tcgcttagtt
     3181 ttagtgttga cgaaatgaaa accaggccct accagatact aagcacactt cacaacctgc
     3241 atgtgtactt caagcagaac agcgaatttg accgattcga tcaggaaaag cacacgctga
     3301 actggataac tgacaataac cttctcagct caactgaaat ctggcttctg tcaaaactgc
     3361 aggagttagt ggcagaagtg accgaatcat tcgatcaatg ccggttccat gaagctgcca
     3421 aagcggtgga tgcatttgtc attaatcaac tgagccaaac ctatgtacct ataacaagaa
     3481 acataatctg ggacgacagc cctgataacc ttgacagacg gcttgcagta tattcagtga
     3541 taggccatgc gctgatgcag ctgaatataa tgctccatcc gctatctcca ttcataaccg
     3601 agtatctcta cgtcacctgt tttaacagaa ataaaagcat cttgcttgaa agctggccga
     3661 agagagacga gaaattaatc gactccaagg tagaattggc atttgacagt ataaaagaga
     3721 tagtttcgct tgcaaacgca gcaaggaacc ttgcaagtct taagagacgc tggcccataa
     3781 aggaagtgat tatctgtggc caaaatttga agtcgcttga catagaaggg atctctgatg
     3841 tgctaaagag ccaacttaac gcggaccagt acaggctagt agaaatagcc gccggctcgc
     3901 aacttaataa agtggttaac cttcttgata aaaagatgcc aatctctgtc agcatatcac
     3961 ttgttagaaa gaatgttgcg ccaagagtca aggcagacat caatagggtt gttgaagctt
     4021 ttgac
//
LOCUS       DASQTF010000043         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_469917,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000043 DASQTF010000000
VERSION     DASQTF010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469917"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VE308_01075"
     CDS             complement(<1..195)
                     /locus_tag="VE308_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HZA99591.1"
                     /translation="MSQEEQIQQTRRKIFEELTKIVDPEIGVSIMELELIDKVDIDSG
                     KVKVDLHLTSPFCPAVFGFKI"
     gene            628..2106
                     /gene="argH"
                     /locus_tag="VE308_01080"
     CDS             628..2106
                     /gene="argH"
                     /locus_tag="VE308_01080"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZA99592.1"
                     /translation="MYRSRPTEKLKDDVLKFLSSMDQDHSILYYDIIGSEAHSIMLHE
                     KGHITHGELKKILCALEEAKKNPTSIRTEEYEDIHEALEAFVIQSSGIDAGGKMQMAR
                     SRNDQVVLDIRMKIRDDINEICAALSNLIEGFVERAKESKQVPMLMYTHLQQAQIGTF
                     SHFLLSYAYGLMRDMERLYLTYHRINQSPLGACAIGGSSIPIDRKRTAMLLGFDSIIK
                     NSIDATSSRDAFLEYASTLAILTSTLGRIAEDFIIWSTTEFGYIELAESYTSTSSAMP
                     QKKNPDPLELTRSKAAIVTSNLVSMLGIVKALPSGYSRDLQDIKAPLHGSSAITLDTI
                     KMMDRVVRSLQVNKEHMRLAASTSYAIAVDIAEQLVMQKNIPFRTAHRIVGALVSKAA
                     SKGNIPLVKLEFADASSVLDAHKVNVKPDELMKIINEMTPEKSLETRKSSGSPSLLEQ
                     EEMIKSLSQGIFNYSTGIQKRTKMVEGSFVNLERTVKNYLQS"
BASE COUNT          725 a          490 c          510 g          598 t
ORIGIN      
        1 tatcttaaag ccaaagactg cagggcagaa tgggctagtc aaatgcaagt caaccttgac
       61 cttgcctgaa tcaatgtcca ccttgtcgat aagctcaagc tccattatcg aaactccaat
      121 ttcagggtcg actatcttgg ttaactcttc aaagattttt cttcgagtct gctgtatctg
      181 ctcttcctga ctcattatgt aagaatttct cgcatactat atttaactat tctataaaag
      241 gcattatgat aatgccaatt attattagta aattagttag tttataaagc cattagacgc
      301 ggttgctttt cctcatcttc ctggggctat atgaagaatc aaaaagtact ttatccctgg
      361 tgcaattgca acatttccaa gcgcgcttaa tggacttata acaaataatg tgttactagg
      421 tatcggtatt gctctcggaa tagcatcaat tgatcttggg attaagagct tcgacagaga
      481 gaatcgaccg tgctagctca gaaggataag gcgagattcg gtgcatattc aatttccaaa
      541 aacacgagaa tagcctaata tcgataaccc tgcggaaagt ctacaacctt aaaataaacc
      601 gcctgcggtg cacatttgat acaatatatg tataggtccc gtcccacgga gaaactcaag
      661 gatgatgtac tcaagttcct ttcttcaatg gaccaagacc attcgatatt gtattatgac
      721 atcattggaa gcgaggcaca ttcaataatg ctccatgaaa aggggcacat tacacatggc
      781 gaattaaaaa agatcctgtg tgctcttgag gaggcaaaga aaaacccaac tagcatcagg
      841 accgaagaat atgaggacat tcatgaagcc cttgaagcgt ttgttataca aagttccgga
      901 atagacgctg gaggcaagat gcaaatggca agatcaagaa acgaccaagt ggtgctcgat
      961 ataaggatga agatacgtga cgatatcaac gaaatttgtg cggctttatc gaacctcata
     1021 gagggattcg tggagagagc aaaagagagc aagcaggttc cgatgctaat gtacacccat
     1081 ttacaacagg cccaaatagg tacattctcg catttcttat tgtcgtacgc ctatggcctc
     1141 atgcgcgata tggagcggct gtatctgaca tatcatagga taaaccagtc gccacttggg
     1201 gcatgcgcga taggaggctc gtctatccct atcgacagga aaagaaccgc aatgctgctc
     1261 ggctttgata gcataataaa gaattctatt gatgctacca gctcacggga tgcatttcta
     1321 gagtatgctt caacacttgc aatactcaca agcacgcttg gaaggattgc agaggatttc
     1381 attatctggt caaccaccga atttggctac atagagcttg ccgaatcata tacgtctaca
     1441 tcaagtgcaa tgccgcaaaa gaagaaccct gatcccctgg agctgacaag gtcaaaggca
     1501 gcgattgtca ctagcaacct tgtgtcaatg cttggcatag tcaaggctct gccatccggc
     1561 tacagtcgag acttacagga catcaaggcg ccattgcatg gatcttctgc tataaccctt
     1621 gacacaatca aaatgatgga ccgtgtggta agatcgttgc aggtaaataa agaacacatg
     1681 cggctggctg caagtaccag ctacgcaatt gcagtggaca tagcagagca gcttgtgatg
     1741 caaaagaaca tacctttcag gaccgctcac agaatagttg gagccttagt gagcaaggct
     1801 gcaagcaagg gcaacatacc tcttgtcaag ttagaatttg ctgatgctag ttcagtcttg
     1861 gatgctcata aagtgaatgt caagccagat gaactgatga aaataatcaa tgagatgact
     1921 ccggaaaagt cgcttgaaac tcgaaaatca tcagggtcac ctagcctgct ggaacaggaa
     1981 gagatgataa aatcgttgtc tcagggcata ttcaattaca gcaccggaat tcaaaaacga
     2041 accaagatgg tcgagggttc atttgtcaac ctcgagagaa ccgtgaagaa ttacctccag
     2101 tcgtgaatgt tatcgcaccg tgcggcaata ttttgccgaa attcggcatc ggtcgatctg
     2161 ttttttggcg attcagtgat taaatagacc aaaatacgtt attttagatg cgataacgtt
     2221 tatattatat ccggaagtag ttgattatcg cataacatgt cactaaaatc agaggatcca
     2281 aactactatg cgcatcagta taactggatg ggccaaactg agc
//
LOCUS       DASQTF010000044         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1025412,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000044 DASQTF010000000
VERSION     DASQTF010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025412"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..709
                     /locus_tag="VE308_01085"
     CDS             536..709
                     /locus_tag="VE308_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99593.1"
                     /translation="MSQMINLVCELRIYITHYKQEYLDGRYLVYGVVSAFTVYVRHDY
                     ISQPRLSDSSEDD"
     gene            1468..1755
                     /locus_tag="VE308_01090"
     CDS             1468..1755
                     /locus_tag="VE308_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S26e"
                     /protein_id="HZA99594.1"
                     /translation="MPKKRASRGRSKGGKGSSGTVSCSQCGALVPRDKAKKVTGRITL
                     VEPTLAKELRASGAYIPMSTDVKFYCVSCAVHRGIVKVRSESDRRTATKLR"
     gene            complement(1742..>2407)
                     /locus_tag="VE308_01095"
     CDS             complement(1742..>2407)
                     /locus_tag="VE308_01095"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZA99595.1"
                     /translation="SFIVYKLLYKNHVENSLLNKLRNLLEPALTKIGITFASTGLSAN
                     FWTGLGLAVSILAAIVYTSNAFLDLDWAPWNFAAAIGGIFLLLSGFFDIVDGSVARVT
                     KQISKKGAFLDSVFDKIAEVIIFGGIALGQLADSFWCLVALGLSLLVSYTRARAESLG
                     GRLKGIGIGERAERMLIIAIIGMIPVREAMQWAMIIVSIVAGITLAQRMAITVRRLSS
                     SEA"
BASE COUNT          731 a          511 c          499 g          666 t
ORIGIN      
        1 aaacccaaac ctcctgcttg tgattccacc gtagcatggt ggtcccccga tgctttctgt
       61 caccgtgcca tcagcacttt ttattttgtc caacacaata tgtgaggcaa tagctattct
      121 caaggtcctt tagctgtaca ttattatttt aggttgataa taaaagggct tgtctgcgca
      181 gcccaaaacc gtggattggg atggcggcgt gggcttgcgc ttgcgccctt gtatttgtgt
      241 ctgtaatcta ccagagctat aaacattgtg attgattgta gcaagatact ttagtctagt
      301 atttttctgt agaatgataa gatttcaaga cagctcgatc gacatgggca ttggcaataa
      361 ttttcttacc acgatagctg tatttctccg aaaaaataac aggatttctc agcagacgta
      421 tgccaatatt attgggatac tataatatgc tgccgtaaga aagcaaggtc agcagtaagt
      481 atctcctata ttgatttagc gcattcaact ataccacgtg caccataatt aatacatgag
      541 tcaaatgata aaccttgtat gcgaattacg aatatacatc acgcattaca agcaggaata
      601 tctggatggg agatacctag tttacggagt cgtttccgct tttacagtgt atgtcaggca
      661 tgactacatc tctcagccaa gactgtcaga ttcttcagaa gacgattaag agtttaacaa
      721 tggttcccaa tggtgcaatc gacgggttca aactcattat acccaaggct accatgccat
      781 acaggatttt tattttactg cagttattct atcagagcaa ggactacaaa cggcagtcga
      841 atcactctgt agtgctacag aattagcaac ctctgcccaa ctatgggtct ctacgtctca
      901 acagtttttc gatgagaaga agaaatagca cacaactatt gaccagatgt attcagggac
      961 gtcaaaaggg ctcatcctat tgaattgcat aataactttt gcaacttgaa aacacttaga
     1021 gaagcaggta gataatatga aatcggaatt agatctaaga caagacaaag caaatgagca
     1081 tcttgatact accaaaatga tgatagaaca tgcaaaaaag cttctaaatc tgtccgatta
     1141 accttcaact tttactttcc tgaaacgggt gctatcctct ctgtctgacg cggcaatgtt
     1201 ctaagacgcc tgccggtatc agctattctt tgactccttt gttttttata gataaaagtg
     1261 cattaaagga ttgacggttc atttttggca atatcatatg taggtaaagc cgatattgta
     1321 cttttaccga ctggagttca aaattgcttc tgcagaaaat aatgagactt ttttcgcaaa
     1381 attcttgatg ttgggtcggc aaaacttaac cattattcaa tttcaaacac aagatttaaa
     1441 ggatagcaaa gaattcttac gcaatatatg ccgaagaaga gagcaagcag gggaaggtcg
     1501 aagggaggca agggtagctc aggcacagta agttgcagcc agtgcggagc tttggttcct
     1561 cgagacaagg ccaagaaagt aacaggaagg atcacccttg tagaacctac gcttgcaaaa
     1621 gaattgagag catcaggagc atacataccc atgtctacgg atgtcaagtt ctactgcgtg
     1681 tcgtgtgccg ttcatagggg tattgtgaag gtaagatcag agtcggatag acgcacggcg
     1741 actaagcttc gctagaagat agtctcctca cagttatagc catcctctga gctaacgtga
     1801 ttcctgctac gatggaaaca attatcattg cccattgcat cgcctctctc acaggtatca
     1861 tgcctattat tgcaatgatt agcattcgtt ctgcgcgctc ccctattcca atccctttga
     1921 gtctgccccc gagggattca gcccttgctc ttgtatagct tacaagaagg gacaacccca
     1981 aggcaactaa acaccagaat gaatctgcaa gttgccctaa cgcaatgccg ccgaaaataa
     2041 ttacttcagc tatcttgtca aagacagagt caaggaatgc accctttttt gatatctgtt
     2101 ttgtaacgcg cgccacgcta ccatcgacaa tatcaaaaaa gcccgaaagc aagagaaaga
     2161 ttccgcctat ggcagcagca aaattccagg gcgcccagtc aagatccagg aaggcattgg
     2221 aggtatagac aattgctgct aggatcgata ctgcgagacc cagtccagtc caaaagttag
     2281 ctgagagccc tgtcgaggca aaagttatgc caatttttgt tagtgcaggc tctagcaaat
     2341 ttctgagttt gttcagcaat gaattttcaa catgattctt atataataat ttatatacaa
     2401 taaaaga
//
LOCUS       DASQTF010000045         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1025704,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000045 DASQTF010000000
VERSION     DASQTF010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025704"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(595..1176)
                     /locus_tag="VE308_01100"
     CDS             complement(595..1176)
                     /locus_tag="VE308_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZA99596.1"
                     /translation="MVFANPFYTNIKIVLDPRYTTMGKKSLEIAGPNVDKNVGMLHRA
                     YSFEMQTSLYFQYVANNIEGIGVLLDDFFGERAKEEQGHAAEINDRLMELGENPTDDP
                     GSWEKESGLGKTQAKKYLSLRSALERALELERFGIGLYNDLANATKDKDHGTYQLALK
                     LLTDELEDEQTIEDILARLEIKDVREGGGGAAA"
     gene            complement(1215..1323)
                     /locus_tag="VE308_01105"
     tRNA            complement(1215..1323)
                     /locus_tag="VE308_01105"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1285..1287),aa:Glu,seq:ctc)
     gene            1666..2802
                     /locus_tag="VE308_01110"
     CDS             1666..2802
                     /locus_tag="VE308_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZA99597.1"
                     /translation="MGIPEKIKEIQEQIHNTQINKATEFHIGLLKAKIARLKREMQEN
                     VHGKTMHTGGENVGFDVRKAGDATVVLIGLPSVGKSTLLNSLTNAKSRVASYQFTTLT
                     AVPGMLHYRGAKIQVLDLPGIIEGASGGKGFGKRVLSVARSADLVLIVLDVFQPHHHS
                     VLKKELAEAGIRLDEQPPNILVERTSIGGISVNAQVPIKASERLIKEIMRVYGLHNGR
                     VIIREPNLTDDQLIDVLNGNRIYVPSVIVLNKIDLVNPSFIQEIKSKIGSNNNDNFIA
                     VSADTGINIDVLKEAIYQRLGFIRVYMRPKGGETDYKEPMIVKNGSTVQDVCNKVHRN
                     MAKNFRYGLVWGKSAKFAGQKVGLDHKLSDEDVLTIVKVTGTST"
     gene            complement(2879..>3198)
                     /locus_tag="VE308_01115"
     CDS             complement(2879..>3198)
                     /locus_tag="VE308_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99598.1"
                     /translation="LNPEREVDVPVEDILLIAQRSAFNDTNPEVYVALNAPTKLVVRN
                     NDVVTHDLKVDASSNDGITPINTAPLRGGQDFLTAIVASKPGTYEYYCSYHPQMRGYI
                     IGR"
BASE COUNT          921 a          699 c          677 g          901 t
ORIGIN      
        1 accttaacca cttcatgttt tctttatgaa gctagattct ccagctatct cgattgaacg
       61 agtattatga gttcaagaga aaagcccaag acatgtcatg tatatgtatc tgattctctt
      121 gcattaaaaa gaagataaaa cctgtcagag atacttattc cagtaacgtt cataggaact
      181 taatatttta tgttccactc cgttcgtgtg tattaattat ttttgatttc tttcacagct
      241 cttcttcatc aggctctgct actaaagccc cggagttgta atgcataaat atatctttac
      301 tcaacacgtg acaaatgccg acagacgaaa tcaagcaacg agaaacctat tgtacatcct
      361 gcaaacaaaa tgttcctatt gctgacgcag cttatgttag actcgccaac ggaagggcta
      421 tcattaaggg aaaatgttca aggtgcgatt tagagttgat caaagcaagc gtaatgccga
      481 aatcatctgc cttaaaatta cttatcaaaa agaggaaaag agcaaagaga gctttctcga
      541 ttccttgatt tcagttactt gttttactct tttcttttgt gatttatctc cttcttatgc
      601 tgctgctcct ccgccgccct ctcttacgtc cttaatttca agccttgcca aaatgtcctc
      661 aatagtttgc tcatcttcaa gttcgtccgt cagtaatttc agtgcaagct ggtaggttcc
      721 gtgatctttg tcttttgttg catttgcaag atcattgtaa agcccgattc caaacctttc
      781 caactctaga gccctctcaa gcgcgctcct aagggaaaga tatttcttag cttgcgtctt
      841 ccctaatccg ctttccttct cccatgaacc ggggtcgtct gtcgggtttt ctccaagttc
      901 catcaacctg tcattaatct cagctgcatg tccttgctcc tcttttgcac gctctccaaa
      961 aaagtcgtcc aataagactc ctattccctc tatgttgtta gctacatact gaaagtagag
     1021 agacgtctgc atttcaaatg aataggctct gtgcagcatt cccacatttt tgtcaacgtt
     1081 aggtccagct atttctaagg atttctttcc cattgtagta tatctcggat ctaaaactat
     1141 ttttatgtta gtataaaagg ggtttgcaaa gaccaaaatg tctatttctt gccatagaaa
     1201 tcctcaaaaa aaagtgctcc ggacgggatg tatccccact ggagatataa cctcgcagtc
     1261 gtttgaaccc gtgatctccg ccttgagagg gcgaaatgct taaccggact acaccaccgg
     1321 agccctgaca gtctcaataa cctacagaat ataaattaag ctttgggacg actgcagaga
     1381 attcttctaa aggccgggtg cttgaactga tatgtaaatc tttgatagca tattggttat
     1441 cattttttcc tgggcgtctt ttttttagga aaaagcttgc caataccagc aagagagtca
     1501 actcttaaaa agacgatatg caactactat aagagtcata tcctaccatt cccaagaaga
     1561 gaactttttg cgtctataca cttccttggc ttgaccaaag catggaaatt gctctaactg
     1621 acaaaatttt tatcgccatg cagacaatga taatgacctc taacaatggg catcccagag
     1681 aagatcaagg agatacagga gcagatacat aacacccaga tcaataaggc tacggagttt
     1741 cacattggtc tactcaaggc caagatagca cgattgaagc gagagatgca ggaaaatgta
     1801 cacggtaaga cgatgcatac gggtggtgaa aatgttggct ttgatgtcag aaaggcaggt
     1861 gacgcgactg tggtgctaat aggcttgcca agcgtaggca aatcgacact tctcaatagc
     1921 cttacaaacg ccaagtcgcg tgtagcgtcc tatcagttca caacacttac ggctgtccct
     1981 ggcatgctgc attacagagg agcaaagata caggtgcttg acctaccggg gatcattgag
     2041 ggggcttctg gcggcaaggg atttggtaag cgtgtactat ctgttgctcg cagtgcagat
     2101 ctagttctga ttgtgctcga tgtctttcag ccacatcacc acagcgtact aaaaaaagag
     2161 ctggctgaag ctggaatcag attggacgag cagccgccaa acatattggt cgaaaggaca
     2221 tcaattggcg gcatatctgt caatgcgcaa gtacctatca aggcttctga aaggctgata
     2281 aaggaaataa tgagagttta cgggttgcac aatggtcgag ttataataag ggaaccaaac
     2341 cttactgatg atcaactaat agatgtgctc aatggcaacc gcatatacgt cccttcagtt
     2401 attgtgctca ataagataga cctcgtcaac ccaagcttta ttcaggagat caagtcaaag
     2461 ataggaagca acaataatga caactttatc gctgtctctg cagataccgg tattaacatc
     2521 gatgtcttaa aagaggcgat atatcaaagg cttggattca tcagagttta catgcggcct
     2581 aaaggaggtg agaccgatta caaagagccc atgatagtaa agaacggttc aactgtacag
     2641 gatgtatgca ataaggtgca ccgaaacatg gccaagaact tccgttatgg acttgtatgg
     2701 ggtaagagcg cgaaatttgc tggacaaaag gtcggccttg atcacaaact atccgatgaa
     2761 gatgtactca cgatagttaa ggttacaggg acatctacct aaaggtacag aactcctaga
     2821 ctgtcggcta ataataattc tcatagaaca agaattcttt gcagatgttt ctgtgccatt
     2881 atcggccgat aatatatccc cgcatctgcg gatgataaga gcagtaatat tcgtatgtgc
     2941 caggttttga tgctacgata gcagtaagaa agtcctgccc gccgcggagc ggagcagtgt
     3001 ttatcggtgt tattccgtcg tttgaggaag catcaacttt caggtcatgg gttaccacgt
     3061 cattgtttcg cacgaccagc ttcgttggag cgtttagggc cacgtagact tcggggttag
     3121 tatcgttaaa cgcacttctt tgcgctatga ggagaatatc ctcgacaggt acatcaactt
     3181 ctcgctcagg attcagga
//
LOCUS       DASQTF010000046         6726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_562925,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000046 DASQTF010000000
VERSION     DASQTF010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562925"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VE308_01120"
     CDS             <1..879
                     /locus_tag="VE308_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZA99599.1"
                     /translation="HYLGIAPEWISVGNGAVEVIYWFVQAVFVKGRVVIPAPTFCEYE
                     LASQKAGAEVTFVPLNNNLELEADQVIEKARGADAVFLCNPNNPTGILATRQIMKIIE
                     NVDSSTRILLDECFIELVDNPNANTMIGQISEFDNLVILRSLTKSFGLAGLRVGYGVC
                     NPTLAKKLSVNKIPWNVNGLAQVAGVAALLERRRYLSKARTLVKKERRFLHDNIDKLE
                     SFRPLRSDSNYFLIHLQDRNSTQFRDRLLKKTGVLVRDCSTFTGMGTQYIRLAVKMHR
                     ENMLLLKALEAFDRND"
     gene            896..1723
                     /locus_tag="VE308_01125"
     CDS             896..1723
                     /locus_tag="VE308_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HZA99600.1"
                     /translation="MVQGTTSGAGKSTVAIALCRLFSDRGYNVAPFKAQNMSSNFFTT
                     SSGSKMALVQAIQAVAARKEPDPRMNPILLKPLGEYRSTVFLNGRFYSDMYAKEYYEK
                     FVIQQGFTMVLKALDSLRSENDVIVIEGAGSPSEINIAKYDIANMLLAHEVGAPVIIV
                     ADIERGGCFASIVGTAQLLKPAHRAIVKGFLINKFRGDITLIAPAIKEVQKITKKRIL
                     GIIPKIEFNLPEEDSLVGSVAGKAEVPRESWNWQIDLIAKAIKENIDMERMSKVVGL"
     gene            1724..2683
                     /locus_tag="VE308_01130"
     CDS             1724..2683
                     /locus_tag="VE308_01130"
                     /inference="COORDINATES: protein motif:HMM:NF002281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HZA99601.1"
                     /translation="MLYLLGALAGGIGIDWLFGDPPNRYHPVAWLGQLIGFFLPRLKG
                     GSEKAKGTIFVISLIIIVALAVHFLVFTSRYLAGLIELVIVSAILFKITIAIRGMERH
                     TQAIINCLEVCDLEATRHNLSMIVRRKTDDLDEQHILSATIECISESTVDGIAGPIFY
                     YSLFGPAGAFAYRAINTLDSMVGYSDDYFKDIGWMSARLDTAANYIPARVTALLMVVS
                     AKILGADWKNSLNILQRDRDKTFSLNAGYPMATMAGALRVRLEKIGHYTLGDNQEPVT
                     VEKCKKAISIMKLTTLLFCIVVSVPVMSVLYLAGWWRFVLGIP"
     gene            2670..3446
                     /gene="cobS"
                     /locus_tag="VE308_01135"
     CDS             2670..3446
                     /gene="cobS"
                     /locus_tag="VE308_01135"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HZA99602.1"
                     /translation="MASLKPVQSVLAFLTILPVGKRNQDIYYIAKNMYLFPVAGLIIG
                     SIIGAMALGIFNFLHPLLIGFLVTGAFIIITGVHHTDALADFADGLMAKGSKEVKHKA
                     MLDPAVGSAGVAVLVMYFVGMVIVFNIGFASGLAIFTSIITAEVIAKYVMVLLANRGL
                     SAWEGFSSPFIAAMKDKHKMLAATCIMVAIVGFATSYAGFAALVISLIIAQVLKYVSS
                     KSFGGISGDVLGASNEITRLSSLIVLSSLSSSMPQVILIF"
     gene            3443..4039
                     /locus_tag="VE308_01140"
     CDS             3443..4039
                     /locus_tag="VE308_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZA99603.1"
                     /translation="MIAVVMCGGRASRMQQQKGMEKPLLKVDGVVMVERVILALASTD
                     RFDRIVAAVSPNTPKTNEFLKSKGIEIIETAGDGYSKDLSYLLSKLKPQKVMTVPGDI
                     PLLKSQIVNEILNTIDDRQDQEPAISIMLEKGFVESIGVKPSIILNQYCHSGITIFNT
                     MAVGTEPVEEGYLVMNRKEIALNVNTKEELELAEKLLV"
     gene            complement(4028..4228)
                     /locus_tag="VE308_01145"
     CDS             complement(4028..4228)
                     /locus_tag="VE308_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HZA99604.1"
                     /translation="MKRESMMMPKPRSSFLSVQCTKCGEKTVVFSHTTINISCKSCGE
                     LLAECSGSKANIIGKVIEALDQ"
     gene            complement(4239..4514)
                     /locus_tag="VE308_01150"
     CDS             complement(4239..4514)
                     /locus_tag="VE308_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HZA99605.1"
                     /translation="MKMEKEMRKFCPRCKTYTVQSIALYKRGRERTGAEGARRHAEDK
                     KGYGGQKFPELKRTAKTTKKMTLRYTCKTCQFKTMKQGLRIRKMEIA"
     gene            5604..6689
                     /locus_tag="VE308_01155"
     CDS             5604..6689
                     /locus_tag="VE308_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99606.1"
                     /translation="MGLTAIAIAVILLASNFTIINAQPQQVTNQPGGIENGATPAAAT
                     TTPFQSTNDSFSVQVPQGWIIQDLNNTGSVLLEESTQGYAILAQLCPEEQQQGAVLSN
                     ASGSTNSSGINTSSCQGAQDVIHIVRYPDLDARIQPANNITAYHLQKLQEVGYSGIQT
                     VTSIDTTVNLTNPLTNEAITTVPAKFVEMTYTTAIAPNETRNGYLISTATNATAPNSG
                     TTKGYTIFYEGNSTTAAAAAEITAASGSLLPPLPPAVGQVFGSFQLIVAPEVTQAAET
                     TEDTTDNDDDDDADDDGDDNGGDDDDGDDNGGDDDGGNGDDDGGNGDDDGGNGDDDGG
                     NGGGGASDDEFDDCVVPPGRDPGDVGC"
BASE COUNT         1935 a         1434 c         1545 g         1812 t
ORIGIN      
        1 cattacctgg gaattgcccc ggaatggatt agcgtgggaa atggagcggt cgaagttatc
       61 tattggtttg tacaggcagt gtttgtaaaa gggcgcgtag ttatcccagc accaactttt
      121 tgtgaatacg agcttgcatc acagaaagcg ggtgcagaag tgacatttgt accactaaat
      181 aataatttgg agcttgaggc cgatcaagta attgaaaaag caagaggagc cgacgcagta
      241 tttctttgca atccaaacaa ccctacaggc atacttgcca cccggcagat aatgaaaata
      301 attgaaaatg tcgatagctc gacaagaatc ctacttgatg aatgctttat cgagctggta
      361 gacaatccta atgctaacac aatgattggg cagatatcgg aatttgataa cctcgtgatc
      421 ctgaggtcac tgaccaagtc atttggactc gccggcctgc gagtcggtta tggcgtgtgc
      481 aacccaactc ttgcaaagaa attgtcagta aataagatcc cgtggaatgt aaatggtctt
      541 gcacaggtgg caggagtcgc tgctctacta gaaagaagaa gatatttatc aaaagcaaga
      601 acacttgtca aaaaagagcg aaggtttttg catgacaata ttgacaaatt agaatcattt
      661 cgcccattaa gatcagactc aaactatttt cttatccacc tgcaagacag aaattccaca
      721 caatttagag atagattgct aaagaaaact ggtgtgcttg ttagagactg tagcacattc
      781 accggtatgg gcacccaata catcagatta gcggtcaaga tgcataggga aaacatgcta
      841 ttgttaaaag cgctggaggc ttttgatcgt aatgactagg cgcgccaagc ttattatggt
      901 acagggtacc acgtctggtg caggcaagag cacagttgcg atagcactat gcaggctttt
      961 ttcggatcga ggctacaatg tagcaccatt caaagcacag aacatgtcct ctaacttttt
     1021 cactacatcc agtggatcca agatggcact ggtccaagcg atacaggcag ttgcagcaag
     1081 aaaagagcct gacccaagga tgaatcccat tctactcaag ccgctcggcg agtatagaag
     1141 tacggtattc ctaaacggtc gcttctactc tgacatgtat gcaaaagagt attacgagaa
     1201 gtttgtgatc cagcagggct ttacgatggt gttgaaagcc cttgacagtc taaggagcga
     1261 aaacgacgtt atagtcattg aaggtgccgg ctcgccctca gagatcaaca ttgcaaaata
     1321 tgatattgcc aatatgctgc ttgcacatga ggtaggagcg cctgtaataa tcgtagccga
     1381 catcgagcga ggtggctgtt ttgcaagcat tgtcggcacg gcgcagctgt taaagccagc
     1441 ccacagggcg atagttaaag gctttctaat aaacaagttc agaggtgaca tcacgcttat
     1501 agcaccagcg atcaaggaag ttcagaagat tacaaagaaa agaatccttg gcataattcc
     1561 taagatcgag ttcaacctgc ctgaagagga ttcccttgtt ggcagtgttg caggtaaggc
     1621 agaggttcca cgagaatcat ggaactggca gatagacctt attgcaaagg caataaaaga
     1681 gaacattgac atggaaagga tgtcaaaggt tgtggggctc tagatgctat accttcttgg
     1741 ggcacttgca ggtggcattg gcattgattg gctgtttggt gatccgccaa atagatatca
     1801 tccggtagcg tggcttgggc aactgatcgg cttttttctg ccaaggctta aagggggttc
     1861 tgaaaaagcc aagggaacga tctttgttat ttcactcatt atcatagtag ctttagcagt
     1921 tcattttctt gtctttacaa gcagatatct tgcaggcctc attgagcttg ttattgtgtc
     1981 tgctatatta ttcaagatta caattgcaat aagaggcatg gagcggcata ctcaggcaat
     2041 catcaactgc ctagaggttt gtgacttgga agcaaccagg cataatttgt caatgatagt
     2101 aagaagaaaa accgatgacc ttgacgagca gcacatcctt tcggcaacta ttgaatgtat
     2161 aagcgaaagc acagtcgatg gaattgcagg gcctatcttc tattattcac tttttggtcc
     2221 tgccggcgct tttgcctaca gggcaataaa tacacttgat tctatggttg gctactctga
     2281 tgactatttc aaggatatcg ggtggatgtc tgcaaggctt gacactgctg caaactatat
     2341 tcctgcaaga gtaactgcat tgctgatggt ggtttcggcc aagatacttg gagctgactg
     2401 gaaaaattca cttaacatcc tacagcgaga ccgcgacaag acatttagtc tcaatgctgg
     2461 ctacccaatg gctactatgg caggggcatt gcgtgtcagg cttgaaaaga tagggcacta
     2521 cactcttggc gacaatcaag agcctgttac agttgagaag tgcaagaaag caatctcaat
     2581 aatgaagctc actacattgc tcttctgcat tgtagtatct gtcccagtaa tgtcggttct
     2641 ctatctggca ggctggtgga ggtttgtgct tggcatccct taagcccgtc cagtcagtgc
     2701 ttgcattttt gactatatta ccagtaggca agcggaacca ggacatctat tacattgcca
     2761 agaacatgta cctatttcca gtcgcaggcc tgattatagg ctcaatcatt ggcgcaatgg
     2821 cgcttgggat tttcaatttt ctgcatcctc tgctcattgg atttttggtc actggagcct
     2881 ttataataat aactggggtt caccacaccg atgccctggc tgacttcgct gatgggttga
     2941 tggcaaaggg cagcaaggaa gtcaagcata aggctatgct cgatcctgca gttggctcag
     3001 caggggtggc agtgcttgtg atgtattttg taggtatggt aattgtattc aatatcggct
     3061 ttgcctctgg cctggcgatt tttactagta ttataacggc tgaagtgata gcaaagtatg
     3121 tgatggtgct ccttgccaac agaggtctgt ctgcatggga gggctttagc tcgcccttca
     3181 ttgctgcaat gaaggacaag cacaagatgc ttgcggccac ttgcattatg gttgcaattg
     3241 taggatttgc caccagctat gccggctttg ctgcactggt catttcactt ataattgcac
     3301 aggtcttgaa gtatgtttct agcaagagct ttggcggcat atctggagac gttcttggag
     3361 catcaaatga gataacccgt ctctcttccc ttattgtgct gtcatccttg tcgtcgtcaa
     3421 tgccgcaggt gatattgatc ttttgatagc tgtcgtaatg tgcggcggca gagcaagcag
     3481 aatgcaacaa caaaaaggca tggaaaagcc actgctaaaa gtggatggcg ttgtaatggt
     3541 cgagcgtgta attttagcct tggcaagcac agacaggttt gaccgcattg ttgcagcagt
     3601 ctcgcccaat actccgaaaa caaatgagtt cctgaaatca aaaggcattg agataattga
     3661 gacagctggc gatggctact caaaggacct atcatatctg ctttcaaaat taaagccgca
     3721 aaaggtcatg acagttccgg gtgatattcc actattaaaa tctcagattg taaacgaaat
     3781 tcttaatacc atcgatgaca ggcaagatca agaaccagca atttcgataa tgcttgaaaa
     3841 aggatttgta gagagcatcg gtgtgaagcc aagcataata ttaaatcagt attgccattc
     3901 tggcattacc atattcaata caatggctgt cggcactgaa ccagtggaag aaggctattt
     3961 agtcatgaac cgaaaagaaa ttgcgctaaa cgtgaataca aaagaagagc tagagctggc
     4021 agaaaaatta ctggtctaag gcctctataa ctttaccaat gatgtttgcc ttgctgccag
     4081 agcactctgc caaaagctcg ccgcatgact tgcagcttat attgatggtc gtgtgcgaaa
     4141 acactactgt cttttcgccg cattttgtgc actgaacaga tagaaatgag cttctcggct
     4201 ttggcatcat catgctttca cgcttcatgc cactgccctc atgctatctc catcttgcgg
     4261 atccttagac cttgcttcat cgtcttgaac tggcatgttt tacaagtata gcgtaaagtc
     4321 atcttcttgg tggtcttggc agttctcttg agctcgggga atttctgacc gccgtaaccc
     4381 ttcttgtcct cagcgtgcct acgtgcgcct tcagcccctg tcctctctct tcccctcttg
     4441 tatagggcta tggactggac cgtataagtc ttacaacggg ggcagaactt gcgcatctcc
     4501 ttctccatct tcatatgata tctctctgtg cttgcaaagg gtaatttaat gtatatattt
     4561 ctctaggagg ttttcccctc tcagcgtgta acagcaaatc taatatacaa gacttacaaa
     4621 cccttctgca gcatgatacc gggtttgttc gaatccctcc ttgagcagtc aatagatcca
     4681 agagcatttc cagtatggat acccctgact tttggactag ccgtaggact ggcttacgta
     4741 attatttcgg ccgcgaggcg cagccgctaa aagtggcgcc taaccctttc tttttataag
     4801 acagcttata atagccagca ttgagtatgt gactttcatt ctgggaattt gctaaatagt
     4861 ctaacttgta gaaatgatcc taaagctgtt gtgtgctacc actggctggt ttattcttta
     4921 ttactcaaga acaaattcga aacataattt cgaacataaa aaacaaaatt cgtttgttaa
     4981 tcatctctct ttgtttaatt tagaatttta gaaatcaagt agttttgatt gtgtcgagtc
     5041 ttctgccatt tgtaaacctt ctactttggc cctcctctga tcaacttgtg agatgcagtc
     5101 gctcaattcc tcgtagcagt aaagcaaatt gacatagttg gcatagagag ccttgtaaga
     5161 tgaattgcga agagcaagct gagagatgta aagtctggta atatcaaaac atcatctcat
     5221 caaacttctt tctatcggac ttgctaatgg ctttgcgaaa tggtttccgt tcagcctcct
     5281 ccattgcaag cgcaatccta aatgatggta ttgttatgcc cattactata aaatcaccaa
     5341 tccactactc tgccgccata ccagtcctaa tgaataatga actccaaaac gagttactat
     5401 aatgaataac agctagcacg aaggagatgg aggtgccata tgtgcaaatg agtttagttt
     5461 tgcagcgctg attccgtgaa gtcatctaat tgaatatttt tatagagtca ctaaccaata
     5521 accttatttt agtgtgaaac atctcttctg ttctagccat tatgagaacg tcctcctcct
     5581 tattcgtttt atcagggaca gccatggggc taacggcaat agcaatagct gttattcttt
     5641 tggcttccaa tttcactata attaacgcac aaccacaaca ggtgaccaac caacctggag
     5701 gaattgagaa tggagcaaca ccagcagcag caacaacaac accatttcaa agtacaaatg
     5761 atagcttcag cgtacaggta ccacaaggct ggataattca ggatctgaat aatacaggct
     5821 ctgtgttgtt agaagagtca acacaaggat atgcaatact agcgcaactc tgcccagagg
     5881 aacaacaaca aggagcagta ctttccaatg ctagtggcag cacaaacagc agtggtatca
     5941 acactagcag ctgtcaagga gctcaagacg tcatacacat tgttcggtat cccgacttag
     6001 acgctagaat ccaaccagct aataatataa ctgcatacca tttacaaaag cttcaggagg
     6061 ttggttacag tggcattcag actgtaacta gtatagatac tacagtcaac cttacaaatc
     6121 cactgacaaa cgaagcaata acaacagtgc cagccaagtt tgtagaaatg acatacacga
     6181 ctgctattgc tccaaatgaa acaagaaatg gttatctcat ttcgactgct acaaatgcaa
     6241 cagcccccaa ctcagggaca acaaagggct atactatctt ttatgaaggt aattctacta
     6301 ctgctgcagc tgcagcagaa ataacagcgg catctggcag tttattacca ccactaccgc
     6361 cagcagtcgg gcaggtattt ggttcatttc agttaatagt agcaccagaa gtgacacaag
     6421 cagccgaaac caccgaagac acaacagaca acgacgatga cgatgatgct gatgatgacg
     6481 gtgatgataa tggaggagac gatgatgacg gtgatgataa tggaggagac gatgatggtg
     6541 gaaacggaga cgatgatggt ggaaacggag acgatgatgg tggaaacgga gacgatgatg
     6601 gtggaaacgg tggaggcgga gcaagcgatg atgaatttga tgattgcgta gttcctcccg
     6661 gaagggaccc cggtgatgtt ggctgctgat acacgtgcaa gcgcagactt ctttatcgcc
     6721 gtcagg
//
LOCUS       DASQTF010000047         4919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1026020,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000047 DASQTF010000000
VERSION     DASQTF010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026020"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            894..1118
                     /locus_tag="VE308_01160"
     CDS             894..1118
                     /locus_tag="VE308_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99607.1"
                     /translation="MTPIDEALKKEHPDKKDYWQNELKSLGIEPRRQNELNDKEELEA
                     LAQIIKEVYYQDYKTAYSNQGKDNREEVSR"
     gene            1497..1889
                     /locus_tag="VE308_01165"
     CDS             1497..1889
                     /locus_tag="VE308_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZA99608.1"
                     /translation="MKEINMLGDKDAVTNIAVKNLETAKKFYEETLGLTQIGAEGQEV
                     IFFRSGSSTIYVYKSQYAGTNQATALTWLVGKDIEGVVRKLKGKGVTFEHYDMPDVTR
                     EGDIHVSGNMKVAWFKDPDGNILNIINR"
     gene            2391..4043
                     /locus_tag="VE308_01170"
     CDS             2391..4043
                     /locus_tag="VE308_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZA99609.1"
                     /translation="MPAQASPNIERTEVFYGSEDAMKILLQAMRNVKKEAVTCSDAQS
                     AGFSMTLEPVRQGFIDFKERGVKIRSITEITNENLEYCKQLGSYVQLRHLDGLRGNFA
                     VSETEYVATAIINEKEPVTETIYSNTKAILEQHRYFFETLWNNAIPAEQRIRELEQGI
                     TREFVEVITDGARAAQLIVEFARSVKMEAQLILSQPLAMERAGKLGMWDLLIEAANNG
                     AQIRVISPITNNNEHLAKEIAARAPNIKIFPGPISTAGMFIVDNKRYFRAEDKDPEAS
                     EVANAISVMIYSNNVNGVKSFRSLFEILWQQLEMYEKLSTHNAMQREFINIAAHELRT
                     PIQALLGITDLIESKVDSSKEKVELSRADISMLVRNVTRLERLSANILDASRIEGKTL
                     NLNKEIFDLNQKVLHVIDDLKLTLPRDRKNIKIFSSSLVDKLEIYADKSRIFEVLSNL
                     IRNSIRFSDEEGATITVNTRQKEGYAVVEVKDNGRGIDPEIMPRLFIKFATKSHQGIG
                     LGLYISKSIVEAHGGKIWAENNPDGKGATFTFTLPIQILHEG"
     gene            complement(4313..>4919)
                     /locus_tag="VE308_01175"
     CDS             complement(4313..>4919)
                     /locus_tag="VE308_01175"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HZA99610.1"
                     /translation="SSFASTTALMMAVAALMLSIPVSNTAIAQQQGMTNATAAKPVDG
                     YNLPQGHLNAVRHNFDDPSLRVEHFCKPHDRVMMVCQLYDSNSANATLIGIEYIITQE
                     QYDSLPNRERPYWHAHREELRPERADPRMPELSPEQAQAEIAKMLPTWGKVIITWIPS
                     DDLPSFPPQVQLVDHPFMVNASVSSGGNSTANATSSNSTGQ"
BASE COUNT         1492 a          945 c         1087 g         1395 t
ORIGIN      
        1 gtcatgggac gcagcaaggg aaaacgagcg aacacggcat gcaaagtgga cctggtggga
       61 tttgaacagt tgttgccttt ccatagatat ccgcctatag acagataata aagaacggag
      121 accagaaagc agttgctgag gccgtgggtt caattcccac cctgtccact tctttctgtt
      181 gtacaactac ggtattgtta ttgagctcat tttaggtcct tgtcggacag aatggtcgca
      241 atgtcgtttg cttacaatgc tataatcatg tcgatagcta ttgggatttt ctatggtttt
      301 acaacctcgc ttataataac acctgcactt gctgcgctag gatggattgt aagcgattca
      361 attggtattt ggaaactcgc ctgtacagat gaacttatga cataaagttc agtgtcgatg
      421 taagcaagcg gcaataatgc gtcatcccaa gcgctgaagc atgaaaattg ctgctcatgc
      481 agcagcctct attattatga ctatttgaca ttattattat gccaaggtct ccactggtga
      541 aatcatcatt agtttttcaa tcatcgtgtg cccgcttgaa tgcttatggt cgtcaatatc
      601 actgtgaaaa acaaatatcg ttctgcattc gtggcaaatc catatcgctt ttaggtcatc
      661 tgggtgcatc taatataaat tcgccgaaaa tggatactta ggcatcgaat ttattactta
      721 cccaatcatc ttcatagttc ttgttcaaac acaatacttc gaattaaaat gttgttgctg
      781 cctttatctg atatatctga ataaggattc tctaactatt agaggaattt tgcatccagc
      841 tatcttttct gttttggtta aatctcagtg ttacaaaaag atcgctagcc tttatgacac
      901 caatagatga agcgttaaaa aaagaacatc cagacaagaa agattattgg caaaatgaac
      961 tgaagtcatt gggcatagaa cctagacgac agaatgagct taatgacaaa gaagagcttg
     1021 aggcattggc acaaatcatc aaagaagtct attaccaaga ttacaaaacg gcatatagta
     1081 atcaaggtaa ggacaacaga gaagaagtta gcagataaat catattttga ttgtattatc
     1141 tattcatcgg ctagctctct caaaaccttg cttgaaggta cagggctgcg aagtatgcaa
     1201 aaagaaatac aatggagcgg gtgggatttg aacagtacat tcttcttcca tagctgctgc
     1261 ttttagatag atataggtag gacctggttt aagaaagctg gctgaggttg tggattcaaa
     1321 tcccaccctg tccatttctt ttgttctggt aaaatacggc attgatttga gcttgatttt
     1381 cggttattgt cggacaaatt cagcagcaaa atgagatatt cctttttctt ctcttagttt
     1441 cttctattca ctgtttggag aaggttcaag taaagcagct ctaggtaggt ggagaggtga
     1501 aagaaatcaa tatgttgggc gataaagatg cggtcacgaa tatcgcagtc aagaatttgg
     1561 aaacagccaa gaaattctat gaagagacgc ttggtctgac tcaaatcggc gctgaaggtc
     1621 aagaagtaat cttctttcgg agtggaagtt ccacgattta cgtttacaaa tcgcaatacg
     1681 ccggaaccaa ccaagccaca gccttgacat ggctggtcgg caaggatata gagggtgtcg
     1741 tgcgaaaact caagggcaag ggcgtcactt tcgaacacta cgatatgcct gacgtgaccc
     1801 gagagggcga tattcacgtc tccggcaaca tgaaagtcgc gtggttcaag gaccctgatg
     1861 gcaacatact caacatcatt aacagatagt ggagcattgc gaaaagttaa caaacaggaa
     1921 tcaatggaaa agcctaatcg agttaataat agtcgtctag ctttgtagtg ctcatctctt
     1981 caaattctct tacaatcttt tctgtttcac ttgttcccac accgcttgtt tcaatccgac
     2041 cagctttcta aacgaattcg cagttgcagt tgctggacca cgtggaagtt gaacaatacc
     2101 tattctccca ctgccagtgt tccttttaga tggatagtca tggtttaaca aagctggctg
     2161 agatcgtgag ttcaaatccc gttgctccac tttatcaatc tagttaacta tggcattaat
     2221 ttgagtcatt tttaagcagc agtcggacag aatctcttat cctagactgc aaaatgctgc
     2281 aagattaaag atatttttaa cgattgccaa cattgaaatg ataaaatctt ctatgttatt
     2341 cttcaagtta tttttatatc cgtgtatata ctgcttcgat tgtatatcta ttgccagctc
     2401 aagcgtctcc caatattgaa aggactgaag tattctatgg atcagaagat gccatgaaga
     2461 ttctgctgca agccatgcgg aatgtaaaga aagaagcagt gacttgcagc gacgcccagt
     2521 ctgcgggatt ttcaatgact cttgagccgg taagacaggg ctttattgac tttaaggaaa
     2581 gaggagtgaa aattagatct atcacagaga taaccaatga aaaccttgaa tattgcaaac
     2641 aacttggaag ctatgtccag ctgcgacatc ttgatggtct caggggcaac tttgctgtaa
     2701 gtgagactga atatgttgct acagcgataa tcaacgaaaa agaaccagtt accgaaacta
     2761 tttacagtaa taccaaggca attttagaac aacaccgcta ctttttcgaa acattatgga
     2821 acaatgcaat acctgcagaa caaaggatac gtgagcttga acaaggtata actcgagaat
     2881 ttgttgaagt tattacggat ggtgctaggg ctgctcaatt aattgtagaa tttgccagat
     2941 ctgtgaagat ggaagctcag ctgatacttt ctcagcccct ggcaatggaa cgagcaggaa
     3001 aactaggaat gtgggatctt ttgatagagg cagcaaacaa tggtgcacaa attcgggtga
     3061 taagcccaat aacaaataac aacgaacacc tggctaagga aatagctgca agagcaccaa
     3121 acatcaagat tttccccggt cccatctcta cggcgggaat gtttattgta gataacaaga
     3181 gatactttag agcagaagac aaagacccgg aggctagtga agtggctaat gcaataagtg
     3241 ttatgattta ttcaaacaat gtaaacggag tcaagtcgtt taggtcatta ttcgagatcc
     3301 tctggcagca attggagatg tatgaaaagc taagtactca taacgcaatg cagagggagt
     3361 ttatcaatat cgcagcacat gaattgcgga ctcctatcca ggcactactt ggcattactg
     3421 atcttatcga atctaaggtt gatagtagca aagagaaagt tgaactgtcc agagcagaca
     3481 tttcaatgtt ggtacgtaat gtgacaagat tggagcgcct ctctgcaaat atactggatg
     3541 catccagaat agagggtaag acacttaatc taaataaaga aatttttgat ctaaatcaaa
     3601 aagttctaca tgtaattgat gatctaaagt tgactctccc tagggacaga aaaaacatca
     3661 agatcttttc tagtagtttg gtagataaac tcgaaattta tgcagacaag tccagaatat
     3721 tcgaagtttt atctaacctg atcagaaatt ccattcgatt ctccgatgaa gaaggagcca
     3781 caattacagt taacacgagg cagaaggaag gatatgctgt tgtagaggtg aaggataacg
     3841 gcagaggtat tgaccctgaa ataatgccaa ggctctttat aaaatttgct acaaaatcac
     3901 accaagggat aggacttgga ttgtatatat caaaaagtat agttgaagct catggtggta
     3961 agatatgggc tgaaaacaac cctgatggaa aaggtgcaac ttttacattc acactgccaa
     4021 tccaaatatt acacgagggg tgactcagac caatatagag atatacatta tcagtctctg
     4081 cgatttgcta tgacaagcca agtcccatct gaaaactgag gatagttaaa tgtggaccgg
     4141 gtgagatttg aacaatcttc ttccccatcc gtgacgacta gaatgggctg ccagccgaga
     4201 ggctgaggtc gtgggttcca accccacccg gtccatctta ttctgttata aaattacagt
     4261 attgaattca tattttaaca ggaaaaaaga atgagaggtg aattgaggag tcttattgac
     4321 ctgtgctgtt gctgctcgtt gcattggctg tgctgttgcc gccgctggat actgatgcat
     4381 tcaccataaa gggatggtct actagctgaa cctgtggcgg aaatgatggc aggtcgtcac
     4441 tgggaatcca tgttatgatt actttacccc atgttggaag catctttgca atctcagctt
     4501 gtgcttgttc tggactcaat tctggcatcc ttgggtctgc tctttctggt ctgagctctt
     4561 ccctgtgagc atgccaatat ggcctttctc tgtttggaag ggagtcgtac tgttcctgtg
     4621 tgattatgta ttcaatacca atcagcgtgg cattagctga attggagtcg tagagctggc
     4681 ataccatcat tactctgtca tgtggcttgc aaaagtgttc gactctgagg gatgggtcgt
     4741 caaagttatg ccttactgcg ttcaagtgac cttgtggtaa gttgtaacca tcaactggct
     4801 ttgcagcagt tgcattcgtc attccttgtt gttgagctat tgctgtattc gatacaggaa
     4861 tactgagcat cagagcagct actgccatca taagagctgt ggtagatgca aagctgcta
//
LOCUS       DASQTF010000048         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1026619,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000048 DASQTF010000000
VERSION     DASQTF010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026619"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..292
                     /locus_tag="VE308_01180"
     CDS             92..292
                     /locus_tag="VE308_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99611.1"
                     /translation="MSIAWQRDENENNNNNNNNNNDGETQHFSLDFQVNQKIKAAVSG
                     RGLQPFIKRYFFEFASDHVGSG"
     gene            453..1421
                     /locus_tag="VE308_01185"
     CDS             453..1421
                     /locus_tag="VE308_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZA99612.1"
                     /translation="MRLLLLRVSIRGLLPLFVIIGAFIVIGAVLISSGVLPEERAREF
                     RSLTDVSIASDMKVIKEPMLGDNTFIYAVNDVAQRGIDIAQGDARVRQILDEAKAKEA
                     TVTIAGVQPTVMVDRQSGELLYSSAGQVVITSNWQTIGGTAYSEPQSFATVADKGLES
                     HQQIWHVLVDVDNGQVRQVNQQANRVTSDTINPDIIRTVVNVFAPNAIVVDAGSAVRW
                     INPSNLPHNVVGIINQTTAQDAVDSTGLIIDNSTNSTSNNNNTNDAQTSPSSSSGVTA
                     IDSGFIQQGQSWQYSFNEAGVFNYLCTIHAEEGMRGTLIIAAPTSS"
     gene            1928..>3688
                     /locus_tag="VE308_01190"
     CDS             1928..>3688
                     /locus_tag="VE308_01190"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZA99613.1"
                     /translation="MSKRDTGFIRRQGSFILSNYRVARIYGMHTHDGNDTKKKNFELP
                     GSRAHYAPSLSFTIKHMQLEIEPDLQKHMISCEQRLSIITLRDTDTIELDAAELQVKM
                     VSAAGRLDFRSLDDKLIVKLGRTLKERSEIEIYIFYTAKPRKGFYFVGPDKYYPDKHQ
                     EAWTQGETTEARYWFPCIDHPRVKFSSEISVIVPIGFTAISNGILLKVDKQNKKQVYH
                     WSETNPHPAYLTSIVIGNYVEMKDGKNLYYYVPQEKKQDAPRSFEHTPEIIRFFEEYL
                     GTNYPYDKYSQVTVQDFVYGGMENSSCTTLTLDTLHDEKAHLDFTSDYLISHELAHQW
                     FGDLVTCSDWQHIWLNEGFATYCEALYWEASKGKDEFQYYMMQTADDYLEEARTRYTR
                     PIVTKVYKHPDDLFDRHTYEKGGCVLHMMRHLVGDNYFRRSLRTYLQRFANGNAETDD
                     LRKVFELETGKSLQQFFDQWIFREGHPELKIDFYHNNHIVEIKVKQSQAGEHFEFPLE
                     VEMAFANSRKKIYTFKVRDRESAFQIPVDDEVEWFSVDPEFKILKTISMKAPKEMLLK
                     QLQEGDNVIKRVEAARALRDK"
BASE COUNT         1145 a          739 c          845 g          959 t
ORIGIN      
        1 agggcttctg ttccatatac ccatgaggat atagggaact acgatcgata tatctatcct
       61 gagatgatcc ccaaagtctg ggagataatc aatgtccatc gcttggcaga gggacgaaaa
      121 cgagaataat aataataata ataataacaa caatgatggc gaaacccagc atttttcttt
      181 agattttcag gttaaccaga agattaaagc cgctgtatca gggagaggct tgcagccttt
      241 catcaagcgt tacttttttg aatttgcaag cgaccacgta ggatcgggct aattgattat
      301 gagtctcgat tatatttctg atgctgagtt cgccagattc ggctattcaa tcatcttatc
      361 cttccagata ataagaagga gagtggaatt attgggtcaa ttttgtcttg gatactggaa
      421 aattgtgata taggttacag cctcaagtac taatgaggct actactattg cgggtaagta
      481 tcaggggttt gctgcccctc tttgtcatta ttggcgcatt catagtaatt ggagctgtcc
      541 ttatcagctc aggagttctc cctgaagaaa gggctaggga atttcgttct cttacggatg
      601 tttcaatagc aagcgacatg aaggtaataa aagaaccgat gctaggcgat aatacattca
      661 tttatgcagt caatgacgtc gctcaacgtg gcatcgatat cgcccaaggg gatgcacgag
      721 taagacagat acttgatgaa gcaaaagcaa aggaagctac tgtgacaatt gctggggttc
      781 agcccacagt gatggtagat aggcaaagcg gagagttact ttacagttcc gcaggacagg
      841 tcgtaattac atctaactgg caaacgatag gtggaacagc ttattcagag cctcaaagtt
      901 ttgcaacagt tgcagacaaa ggccttgagt cgcaccagca gatatggcat gtccttgttg
      961 atgtagacaa tggtcaggtc agacaggtta atcagcaggc gaatcgagtc acttctgata
     1021 ccataaatcc cgatattata cgcactgtgg tgaacgtgtt tgcgccaaat gcaattgtag
     1081 tggatgcagg ctctgctgtc aggtggatta atccttcaaa tttgccacac aatgtagtcg
     1141 gcataattaa ccagacaaca gctcaggatg ctgttgatag cactggactg attattgata
     1201 attcaacaaa cagtactagt aacaataata acactaatga cgcacaaacg tcgccatcat
     1261 cttcttcagg tgtaactgca atagatagtg gctttattca gcagggtcaa tcgtggcaat
     1321 acagctttaa tgaagcagga gtctttaact atctatgtac aatccatgca gaggaaggta
     1381 tgaggggaac gctgataatt gcagctccaa cctcctcatg atcgcttgtt ttccattatt
     1441 agttgggtaa aggtacaaga cacattctcg tatgttagat aaacagatca acgcctatca
     1501 tatccatgag gaaataatta ataaaatggg agaatatgtt ggtaagctaa gaccaagata
     1561 ttactagcaa ccagattatt agaacagaag cagaatcaga gaggttaaag agcaatagaa
     1621 gtagtaggag attattccta tcgatggcca tttgctgcac tcctcatcag ggaggccgca
     1681 gcagagcttt ttcgcaattt tctggcgact ataaccaagc ctgtcaacta tggtgcagta
     1741 gtaatatagt tcttcaaaga taagcaaagg aatcttgtct aggtcgtaga gaattcctag
     1801 gttactgata gttggaacaa gctctagtat ctagataatt gtgctacaaa tgattgtcag
     1861 acccaagaac taagctacgc atatgtttcc tccattgcac ttgtctctag agatatctgg
     1921 aatatgtatg tcgaagagag acaccggttt tataaggcga caaggaagct ttattttgtc
     1981 aaactatcga gtagcacgta tatacggaat gcatactcat gatgggaatg acacaaagaa
     2041 aaagaatttc gagcttccag gcagtagagc tcattatgct ccgtcgcttt catttacaat
     2101 taagcacatg cagcttgaga tagagcctga tcttcaaaag catatgatta gttgcgagca
     2161 aaggctgtcg attataactc tgcgggacac tgacaccata gaacttgatg cagctgaact
     2221 ccaggtaaag atggtctctg cagcaggcag gcttgatttt cgtagccttg atgacaagct
     2281 tatagttaaa ctaggcagaa ctctaaagga gaggagtgaa atcgagatct acattttcta
     2341 tactgccaag ccgcgcaagg gtttctactt tgttgggcct gataagtatt atcccgataa
     2401 gcaccaggag gcatggacac agggtgaaac tactgaagca aggtattggt tcccttgtat
     2461 agatcatccc cgagtcaagt tttcaagcga aatttcagtt atagtcccga taggatttac
     2521 ggcaatttca aacggcatac tactaaaggt ggacaagcag aataaaaaac aggtctacca
     2581 ctggtctgaa acaaaccctc acccagccta tcttacatcg atagttatcg gcaactacgt
     2641 tgagatgaag gacggcaaga atttgtacta ctatgtccca caggaaaaaa agcaagacgc
     2701 acccagatca tttgaacata cccctgaaat tatcaggttc tttgaagaat accttggcac
     2761 gaactatcct tatgataaat attcacaggt gacagtccag gattttgttt atggcggtat
     2821 ggaaaattcc agctgcacta cactaacgct ggatacactt cacgacgaaa aagcccacct
     2881 tgactttaca agtgattatc ttatatcaca tgagctggca caccaatggt ttggcgacct
     2941 tgtcacatgc agtgattggc agcatatctg gctcaatgaa ggatttgcca cttactgtga
     3001 ggcactctac tgggaggcaa gcaaaggcaa ggacgagttc cagtattata tgatgcagac
     3061 tgctgacgac tatcttgaag aggccagaac caggtatact aggcccattg taacaaaagt
     3121 atacaagcac cctgatgacc tctttgacag acacacctat gaaaaggggg gatgcgtgct
     3181 ccacatgatg cgccacctcg tcggagacaa ctacttcaga aggtcgctta gaacatattt
     3241 gcagcgcttt gccaacggca atgccgagac ggacgatcta cgaaaagttt ttgaacttga
     3301 aacaggcaag agtctgcagc agttcttcga ccaatggatt tttagagagg gccaccctga
     3361 gctcaagatc gacttttatc ataataacca cattgtagag atcaaagtta aacagtcaca
     3421 ggcaggcgag cattttgagt tcccgcttga agtagagatg gcttttgcaa atagccggaa
     3481 gaaaatatat acattcaagg tccgggatag agagagtgcc ttccaaatcc ctgttgacga
     3541 cgaggtagaa tggttctctg ttgatccaga gttcaagatt ttaaagacga tatctatgaa
     3601 agcgccaaag gagatgcttc tcaagcagct ccaagaaggg gataatgtga tcaagcgtgt
     3661 ggaagctgcc agggcactca gggataag
//
LOCUS       DASQTF010000049         4597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_8209,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000049 DASQTF010000000
VERSION     DASQTF010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8209"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VE308_01195"
     CDS             <1..367
                     /locus_tag="VE308_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia monooxygenase"
                     /protein_id="HZA99614.1"
                     /translation="ALPLGWGFVAIYDRRVPILRGPGNSMNTGLKITIIGYLATMFTI
                     GVNEMWHFWFVEEIFAVPNHWMFNMGVVVAFMGALAYVVRVYARMVELGAETPAKNPY
                     VAEMYKLALEGKLYSRSIP"
     gene            727..870
                     /locus_tag="VE308_01200"
     CDS             727..870
                     /locus_tag="VE308_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZA99615.1"
                     /translation="MPVVDPVCGIEMLQELAISHEHHGRTYYFCCEGCKRIFTKKPGK
                     YSK"
     gene            complement(862..1887)
                     /locus_tag="VE308_01205"
     CDS             complement(862..1887)
                     /locus_tag="VE308_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HZA99616.1"
                     /translation="MTPKNNNNNNNKRAKLGFCVILQDCEQLIKLLTESVQRNHADAL
                     LLSGGLDSSILASILRPEYSLAAGFGGAAPDLVYARQVAERYSKHHVEIVFGQNKMAE
                     LVEQVIQIFKTFDPIEIRNSVVALAGIEWAKSDGYSSIMTGDGSDELFAGYNYFSRYY
                     SDMERFDSELRRLWQIMHFSSRKLGEHAGVGVKTPFLDEKFASFAKLISVNDKVGEHD
                     GKMWGKFILRKCFEQALGSLVWRPKLAQEQGAATDRYQQYIEEMIDNLTFANKKRIAQ
                     EQESVRIRSKEHLHYYAIFRSYFSPPKEEEDDSCISRCSDCRGCIASDARFCRKCGAF
                     PVVPLSL"
     gene            complement(1871..2290)
                     /gene="mce"
                     /locus_tag="VE308_01210"
     CDS             complement(1871..2290)
                     /gene="mce"
                     /locus_tag="VE308_01210"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HZA99617.1"
                     /translation="MRVDHIAIAVNNVQQALTDYQKILNVDELKIEEVPNEKVRVAML
                     MLEDTRIELMEPMAPDSPISRFLQERGEGIHHIAITADNIEKDVARASDNGMKMLGGL
                     RTGSYGRKITFIHPKSLHGVLTEFCEAPPLQSDDAKE"
     gene            complement(2527..3090)
                     /locus_tag="VE308_01215"
     CDS             complement(2527..3090)
                     /locus_tag="VE308_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99618.1"
                     /translation="MSSCLGEAPVVLKIQALLRIIGKLKQGLVKIALSNRPIELGRNI
                     TYLTEFPKSNLMQENKGLDSKKQEEFLWVATFLPLKSWKYLIPFQLMTSKVLKQVKET
                     KGVINYAVKADFPKKHFWTLSIWKDQDSLRHFVMAEPHATAIKKFTEWAGEGSAFVEW
                     TSSDDTIDWTVAIERLKNPTFYYKNQK"
     gene            complement(3191..>4597)
                     /locus_tag="VE308_01220"
     CDS             complement(3191..>4597)
                     /locus_tag="VE308_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZA99619.1"
                     /translation="SAEETNRRFKFLLDHGQTGLSLAFDLPTQTGRDSDDPQAEGEIG
                     RTGVSITSIKDMMTCFDTIPLDKVSTSMTINSTASTLLSLYITVAEKQGVSPDQLRGT
                     TQNDILKEYIARNTYIYPPKPSMRLIGDMIEYCAKKVPQWYPISISGYHMREAGCNAV
                     QELAFTFANAIEYINTCTSRAGLNVDDFAPRLSFFFCCTMEFCEEIAKFRAARRIYAK
                     MMKEKFNAMNPKSQHLRFHVQTSGESLTAQQVDNNIVRVATEALAAVLGGCQSLHTNS
                     RDEALALPTEESVKIALRTQQIIASETGVSKTVDPMGGSYYIEYLTAKIEEEVNEYLK
                     KIERMGGALVAVEKGFFQEEIRNNAYQLKKEIDENKRIIVGVNKYQEEHDIEPKINRI
                     DPLLEQKQLANVKEFKSNRDKSKVDQALSQLQKAAEKDDNNLMPHIINAVKSYSTLGE
                     ISSTLRTVFGRYEPKISF"
BASE COUNT         1166 a         1041 c          943 g         1447 t
ORIGIN      
        1 agcactccca ttaggctggg gctttgtcgc aatctacgac agacgagtac caatcctgag
       61 gggaccaggc aattcaatga acaccggttt gaagattacc atcattggct atttggccac
      121 gatgttcaca atcggtgtca acgagatgtg gcacttctgg ttcgtcgaag aaatcttcgc
      181 agttccgaac cactggatgt ttaacatggg tgtcgttgtg gcattcatgg gtgctctggc
      241 ttatgtagtc agagtatacg ccagaatggt cgaactgggt gcagaaacac cagcaaagaa
      301 cccatacgtg gctgaaatgt acaagctagc acttgaagga aagctgtaca gcaggtctat
      361 tccataaact tgggatttcg acaatgtctg ggaaaccgga cgcccgtttt ttctattttt
      421 tctttttaaa aaaataaaag gttatcaagc actatcaaat aacacaaagt gcttgtatca
      481 ggtgaagtat taagacgcaa ttgccttagg tcttagaata ggtcaatgtc ctgtttccac
      541 ccattctagg cgagaaaact aaaaatcttt aatgcgatat tgaaaaggca actcctttat
      601 cttgaattca gatgcatcac acttcaacaa atgtctaagc cactttctct tttttttggg
      661 aaagataaca cgccaatgat catttttggt aagcaataaa tacgtaattt ctttgcatac
      721 tgcttgatgc ctgttgttga tccagtctgt ggaattgaaa tgcttcagga acttgcaatt
      781 agccatgagc atcatggtag gacttactac ttttgttgtg agggctgcaa gaggatcttt
      841 acaaaaaagc ccggcaaata ttcaaagtga aagtggcaca acaggaaaag caccacactt
      901 tctgcaaaac ctagcatcag atgcaataca tcctcgacaa tccgaacagc gtgatatgca
      961 gctatcatct tcttcctctt ttgggggaga gaaatagctt ctgaaaatgg catagtagtg
     1021 cagatgttcc ttgcttctta ttctgacact ctcctgttcc tgagcaattc ttttcttgtt
     1081 tgcaaaagtc aagttgtcga tcatttcttc aatgtattgc tggtaacgat cagtggcagc
     1141 tccctgctcc tgcgcaagct tgggtctcca taccagtgag cctagtgcct gttcaaagca
     1201 ttttctcaga atgaacttgc cccacatctt accatcatgt tcgcccactt tgtcgtttac
     1261 actgatcaat tttgcaaatg atgcaaactt ctcatcaagg aaaggagttt ttaccccgac
     1321 gccggcatgt tcgcctagtt ttcttgagga aaaatgcatg atctgccaca gccgtcgcaa
     1381 ctctgaatca aacctctcca tgtcagagta atagcgagaa aagtaattgt agcccgcgaa
     1441 cagctcgtcg ctgccgtcac cggtcataat gctagaataa ccatcgcttt ttgcccattc
     1501 gatgccagca agtgccacga cagaattcct gatctctatc ggatcaaatg ttttaaaaat
     1561 ttgtatgacc tgctcgacca gctctgccat tttgttctgc ccaaaaacaa tctcaacatg
     1621 gtgtttgctg tatctttctg caacctgcct tgcataaaca agatcaggag cagctcctcc
     1681 aaatcccgcc gcaagtgagt attctggccg tagtatactt gccaaaatgc tgctgtcaag
     1741 gccgccggag agcagcagtg catcagcgtg atttctctga acgctttctg tcagcagctt
     1801 gataagctgt tcgcaatctt gcaatataac acaaaatccc agttttgctc ttttattatt
     1861 attattatta ttattctttg gcgtcatctg attgtagtgg tggcgcttcg cagaactctg
     1921 tcagtacacc atgaagtgac ttgggatgta taaacgtgat tttacggcca tacgagcctg
     1981 tgcgcaaccc gccaagcatt ttcataccat tgtcagatgc tcttgcaaca tccttttcga
     2041 tgttgtcagc agtgattgct atatggtgta tgccttctcc acgttcttgt aggaatcttg
     2101 aaattggact gtcaggagcc attggctcca tcaattctat tctcgtgtct tcgagcatta
     2161 gcatcgcgac cctgaccttt tcattgggca cttcttctat ttttagctcg tcaacattga
     2221 gtattttttg atagtcggtt aatgcctgtt gcacgttgtt aactgcaata gctatatggt
     2281 cgactctcat ttggtagtag agagagtcaa tgagagttta tttaatctaa tgctattgtt
     2341 tctgctatat cgctaatatt gacgaaaatt cctctgtcaa agccagaaac tttgaaatcg
     2401 ctaatgataa ttcccgtaga atttttgaca tgaattgcta ttgacagttg ctatctcacg
     2461 tgctattatt ttaaggtcga ttactgtatc ttaatattct atatcttcac ctataattac
     2521 agtagattat ttttgattct tatagtaaaa ggtgggattc ttaagccgtt caatagcaac
     2581 tgtccaatca attgtatcat ctgagctagt ccattccaca aatgccgagc cctctccagc
     2641 ccattccgtg aacttcttaa ttgcagttgc atgaggctct gccataacaa agtgcctaag
     2701 agaatcttga tctttccaaa tggatagcgt ccagaagtgc ttttttggaa aatcagcctt
     2761 gacagcatag ttaattacgc ctttggtttc ttttacctgc ttcagtacct ttgaagtcat
     2821 taactgaaaa ggaatcagat acttccaact ctttagcggc aggaaagtgg caacccacag
     2881 aaattcctct tgttttttgg agtctaagcc tttattctcc tgcattagat tgcttttggg
     2941 gaattcagtt aaataggtta tatttctacc caattctatt ggtctattag ataacgctat
     3001 cttgaccaat ccctgcttta gcttcccgat tatcctcagt aatgcctgta ttttcaacac
     3061 gacaggtgcc tctcctaggc agctggacaa tcaaaaccta agcgcctctt tttggttggt
     3121 agaaccgtgg ctaatactac taccacaact agctcgattg ttgcattcgc tctatagtcc
     3181 ttcttacacc ctagaaagat atcttgggtt cgtatctgcc aaacactgtt cgtaaagtgc
     3241 tgctaatttc gccaagtgta ctgtaactct taactgcatt gatgatatga ggcatcaagt
     3301 tattgtcatc cttctcagct gccttttgca gttgtgatag cgcttgatca acctttgact
     3361 tgtcgcgatt gctcttgaat tccttgacat ttgctaattg tttttgctcc agcaatggat
     3421 ctatcctgtt gatcttcggc tcgatatcat gctcctcttg atatttattg acgcctacga
     3481 taatgcgctt gttttcgtca atttctttct tcaattgata tgcgttattt ctgatctctt
     3541 cttggaagaa acccttctcc accgcgacta gagcaccgcc catcctttct atctttttga
     3601 gatactcgtt aacctcttcc tctatcttgg ctgtaaggta ttcgatgtag tatgagccac
     3661 ccatcggatc cacagtttta ctgacgcctg tctcactagc aattatctgt tgagtcctga
     3721 gtgcgatctt gactgattct tcggttggaa gtgcaagtgc ttcatcacgc gaatttgtat
     3781 gcaacgattg gcatccacca agtactgcag caagtgcttc cgttgcgact ctgacaatgt
     3841 tattgtcgac ttgctgggca gtgagtgatt caccggatgt ctgaacgtgg aagcggaggt
     3901 gctgcgattt aggattcatt gcattaaatt tctctttcat cattttggcg tagatccgcc
     3961 gagccgctct gaacttggcg atttcctcac agaattccat agtgcagcag aagaagaacg
     4021 aaagtcgtgg agcaaagtca tcaacattga gtcctgctct gcttgtgcaa gtattaatgt
     4081 attcaattgc gtttgcaaag gtgaaggcta gctcttgtac agcgttacag ccggcttctc
     4141 tcatgtgata gccagaaatg ctgatcggat accattgtgg aactttttta gcgcaatatt
     4201 cgatcatatc acctatcagg cgcatcgatg gcttgggcgg ataaatgtag gtatttcgtg
     4261 cgatgtattc ctttagaata tcgttctgag tcgtgccgcg cagctggtcc ggcgacactc
     4321 cctgcttctc ggctacagtg atgtaaagcg acagaagcgt agatgcggtt gagttgatag
     4381 tcatagaagt gctgacctta tcaagtggta tggtgtcaaa acaggtcatc atatccttta
     4441 tcgatgtgat ggatacacct gtccttccaa tctcaccctc tgcctgcggg tcatctgaat
     4501 ccctgccagt ctgagtaggc aggtcaaatg caagtgaaag gcccgtctgg ccatggtcta
     4561 gcaaaaactt gaagcgcctg tttgtttctt ctgcact
//
LOCUS       DASQTF010000050         5092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_100848,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000050 DASQTF010000000
VERSION     DASQTF010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100848"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..653
                     /locus_tag="VE308_01225"
     CDS             207..653
                     /locus_tag="VE308_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99620.1"
                     /translation="MNSYSGTNDNNNRRTKATAVIPMVGAIVTAALLLSGLSLLSSYQ
                     PVIAQQNMTGGTMDGNATTAGNATTAGGNQTTSEVRMHLEEARTAAQNDDMEGVLMHL
                     DLALNALGGGTQGNMTAGGGNLTAAVEEGGLTAEGGEGGAEEEEEG"
     gene            1219..2382
                     /locus_tag="VE308_01230"
     CDS             1219..2382
                     /locus_tag="VE308_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HZA99621.1"
                     /translation="MKSRCTIDTIFLNIETQLGAWGLEQKGGYEIASNQKSEEGVLGI
                     HHITAIARNPQRNVDFYSGLMGLRLVKLTVNFDDPTTYHLYYGNSLGIPGTILTFFPW
                     SEAPTGYRGTGQVTAISFLIPSGSMTYWIDRLKTNGISFVGPSKRFSDEFVSFHDPDG
                     LMLELISPSSSDSQGQRQLQQETDNNIWKESLISKEHAIRGFHSATLSEEGYERTASL
                     LADTMGFKLVAQDNKEDRFRFGIVEKNNSNQEGASSSTESSPSIGSFVDIVCQPEISR
                     GYIGIGTVHHIAWRAANDRHQIDLRKRIVKEAKLNPTPVIDRTYFHSVYFREPGGILF
                     EIATDPPGFAIDERPEDLGKQLKLPQWLEPMRGKLEQLLPPVKTFSGKAREQE"
     gene            2392..3069
                     /locus_tag="VE308_01235"
     CDS             2392..3069
                     /locus_tag="VE308_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZA99622.1"
                     /translation="MSVPTSSSSANKSDHLGFTHRYIPPLDRNEKAELTLLLLHGTGG
                     NEDDLLPLGRELDPNAGLLSPRGKILEGGRIPRFFRRLAEGVFDIEDLKFRTLELAGF
                     VEKASSIYEFDARRVIAVGYSNGANIAASMLLLKPQTLTGAILFRAMVPLVPDNLPDL
                     SDKRMIMSSGLRDPIATRQEAERLSGLFKQAHAVVDLQWQNSGHELIQEDIHAAKQWL
                     SLNKKQL"
     gene            complement(3116..3442)
                     /locus_tag="VE308_01240"
     CDS             complement(3116..3442)
                     /locus_tag="VE308_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99623.1"
                     /translation="MPNIKYSKHNRVRFKSLVDKDKGRNMFTEFFGSIRKEELGKEMR
                     GHIILDSTSDFRESIILTFWETREDMDKFYSPQNKALASLVERAKPLFEKMPERTDYA
                     VSELSL"
     gene            complement(3728..4345)
                     /locus_tag="VE308_01245"
     CDS             complement(3728..4345)
                     /locus_tag="VE308_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99624.1"
                     /translation="MVTTRKSSSSRTSKKRGKVGSKTNSRSGKVNSQKRRRTTKQSSK
                     ATGRTQRKKRTKASDIIIKRTSGRREKFETDKMAQTVSRSGTPFLLARDVAKTVSEKV
                     MRKGNDTTTAKGKEKRTSSPSTIEVDGGEVRKLVAEELKERNRPDIASSYAGERPENT
                     RQGGHEVMNENQPLHDANAALQSKLLFDSSTQMARSTKSSSALKR"
     gene            complement(4783..>5092)
                     /locus_tag="VE308_01250"
     CDS             complement(4783..>5092)
                     /locus_tag="VE308_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99625.1"
                     /translation="AITTINEYDARQMKVDYSKLTKYQNSLGIGGSAESYLADSCHLY
                     FGDGIESVDVKPVKFLRQSNVAKVDQERYPSLLGMDVLRNYKISFNESKVILERDSRS
                     "
BASE COUNT         1434 a         1086 c         1055 g         1517 t
ORIGIN      
        1 gacaacaaca ggaacgtaaa atggactaga cagacaacgc tatagaaagc agacaatcct
       61 gacttggttg ctcacggcaa ccaagttgag ggcaataaaa ctccctttct cttttttctt
      121 tatttctatt agaatgaata acaaagcttc caatttgtca taatgtacac aaatggttaa
      181 gttctatagc gttcacactc gagctaatga attcatatag tggaacgaat gataacaaca
      241 atagaagaac caaagcaaca gctgttatac cgatggtagg ggcaatagtt actgcagcat
      301 tattgctgtc gggactatct ctccttagca gctaccagcc tgtaatagca cagcagaata
      361 tgacaggtgg aaccatggac ggaaatgcta ctactgcagg aaatgctact actgcaggag
      421 gaaaccagac gacatcagag gtaagaatgc atctagaaga agcacgtacg gcagcgcaga
      481 atgatgacat ggaaggcgta ctgatgcact tggatctagc cttgaatgca ttaggtggcg
      541 gcacacaagg taatatgact gcaggcggcg gtaatctaac tgctgcagtt gaagaaggag
      601 gcctgacagc tgaaggcgga gaaggcggag ctgaagaaga agaagaaggt taagtgatga
      661 cggcagcagt agtgtgctag aatagcttca agacttcttt gaagaggaca gaggcagtca
      721 ctacgtcgtt aacaatacca acaacagaca gggagcttct ctattttttt ctctccttct
      781 aatttttttg ctctatatac atgatgcata tagtctaaag tatatccttg ctatttgttc
      841 tgaaagcctg aaaatgcagt tggatatcat aatcatcgct gcctcattgg cagcttgacc
      901 tgcttttttt gcttggcgct tagctgaacg gtcttgcagg tatatttgtc cagtagtaat
      961 ggacccttgt actggacgat ggatgcaatg gcattttgcc ctaattgtca aagttaagct
     1021 gtggtgtgga cactctcatc aacttagact ttgcttcctg gcctaccttc caaatgaggc
     1081 cttttggcag atgtatttct atttctcttt cttctaatat aacaggattt ctaaagccct
     1141 ggactcccat caagattatg gaatgctagc ccgcaagcaa gcaaaaatac ttcatctatg
     1201 tggatccgtg tactataatt gaagagcaga tgcactattg atacaatctt tttaaatatt
     1261 gaaacccagc ttggggcatg gggattggaa cagaaggggg gttatgagat agcgagtaat
     1321 caaaaatcag aagaaggagt tcttggtatt catcacatta cagctatagc tagaaacccc
     1381 caacggaatg ttgactttta ctcggggctg atggggctga ggctggttaa actaacagtt
     1441 aattttgatg atcctacaac atatcacctt tactatggta attcactcgg aattcctggt
     1501 accattctga cgttcttccc ttggtctgag gcacctacag ggtatcgcgg aacaggacaa
     1561 gttacagcca tatccttttt gattcccagt ggctcgatga cctactggat tgataggctc
     1621 aagaccaatg gcatttcgtt tgttggccct tcaaaacgct ttagcgatga atttgtttca
     1681 ttccacgatc ccgatggatt gatgcttgag cttatttccc cttcctctag tgattctcaa
     1741 ggacaacgac aactgcagca ggagaccgat aacaacatct ggaaagaaag cctcatcagt
     1801 aaagaacatg ccataagagg attccactca gccacccttt ctgaagaagg atatgagcgc
     1861 actgcttcac ttctggctga tactatggga tttaaacttg tagcccaaga caacaaggaa
     1921 gatagatttc gctttggaat agtagagaaa aacaatagca atcaagaagg tgccagcagc
     1981 agtacagaat cctccccatc gataggctca tttgtcgaca ttgtatgtca acctgaaata
     2041 tcgagaggct atataggaat aggcacagtc caccatattg cgtggcgcgc ggctaacgac
     2101 aggcatcaga tagatttgcg taagagaatt gtcaaggaag caaagcttaa tcctactcct
     2161 gtaattgacc gcacttactt tcattctgtc tattttcgag agccaggtgg catactcttc
     2221 gaaattgcca cagatccacc tggatttgct atagatgaaa ggccagaaga tcttggaaag
     2281 caattaaaac taccacagtg gcttgagccc atgcgtggaa agctggaaca actgttgcct
     2341 ccagtaaaaa ccttctccgg aaaggcaaga gaacaagaat aggaggacat aatgagtgtg
     2401 ccgacatcat catcgtcagc caacaaatca gaccacctag gctttacgca ccgctatatt
     2461 ccgcctctgg atagaaatga aaaagctgaa ttaaccctgc ttctgttgca tggcactggc
     2521 ggaaacgagg atgaccttct gccgctaggg cgtgagcttg accccaacgc tggattgcta
     2581 agtcctcggg gcaaaatact agaaggaggc agaataccgc gtttctttcg caggcttgcc
     2641 gaaggcgttt ttgatatcga ggatcttaag tttcgcaccc tagagcttgc cggcttcgtc
     2701 gagaaggctt caagcatcta tgaatttgat gcgcgccgtg tcatagcagt gggatattcc
     2761 aatggtgcaa acatagcagc tagcatgctc ttactcaagc cgcaaacatt gacaggcgca
     2821 atactcttcc gtgccatggt tcctcttgtt cccgacaacc ttcccgacct atcggacaag
     2881 cgcatgatca tgtcttctgg actgagagat ccaatagcta caagacaaga agcagagagg
     2941 ctttctggat tgttcaaaca agctcatgct gttgtagatc tgcaatggca aaacagtggc
     3001 cacgagctaa tacaagagga cattcatgca gccaaacaat ggctttcgct gaataagaag
     3061 caactataga tcaaaggcgg atttacttta tcgattcttg acaaaagtta caatatcata
     3121 atgataattc tgaaacagca taatcagttc tttcaggcat tttttcaaat aagggctttg
     3181 ctctttcgac caagctagcc aaggctttgt tctgcggaga gtaaaacttg tccatatcct
     3241 ctcttgtctc ccaaaaggtc aggatgattg attctcggaa atcgcttgta ctgtcaagta
     3301 taatgtgacc tctcatttcc tttcctaact cttcttttct tatgctacca aaaaactcag
     3361 tgaacatatt tctacccttg tctttatcta ccaaagattt gaagcgaact ctgttgtgct
     3421 tggagtattt gatgtttggc atataggaca ctagagatgt aggtgctaat gtggttctta
     3481 agcattgatt gattacttct cataatcttg aaatgatgta acagaatgag ggaatatgta
     3541 tatgtctaga aggtttcttc ggtcagccat ataatgaccc tttagatatc ttatgagata
     3601 tgaagaaaga aagaaaaaat gcaaagtaag ggctttgcat ttttttcttc tctcgccttt
     3661 tattgacaag aaaaaatatt ggcgttaatg gatttttcaa aatctcgcgg tttttctgtg
     3721 gtttttccta gcgcttcaat gcggatgaag actttgtact tcttgccatt tgtgtgcttg
     3781 aatcaaagag taacttgctc tgaagtgcag catttgcatc atgcaatggt tgattctcgt
     3841 tcattacttc atgccctcct tgccgcgtgt tttctggccg ttcacctgca taagaggacg
     3901 caatatcagg acggtttctt tcctttagct cctctgcgac cagctttctt acctcacctc
     3961 catctacctc gatagtagaa ggcgaagacg tcctcttttc ttttcctttt gctgttgtgg
     4021 tatcgttccc cttacgcatc accttctctg aaactgtctt tgcaacatct cttgcaagca
     4081 agaatggagt acctgacctg cttacagttt gagccatttt gtcggtttca aacttttccc
     4141 ttcttccact tgttcttttg ataataatgt cagaagcctt tgtcctcttc ttcctttgag
     4201 ttctgccagt tgctttggat gattgttttg ttgttcttct tcttttctgt gaattgacct
     4261 ttcctgatct actgttagtc ttggaaccta ccttacctct tttcttggaa gttcgtgatg
     4321 atgagctctt tctcgtcgta accatatcaa aagaagtttg gaaaacaaag ttgatatatg
     4381 ttaagcatat cgggttctgt aactttaggc aatatattga acttcgtaaa taacatgtca
     4441 tttactctga tgactctcgc gttatagcaa gtaccggtgc tctgatgact gtatttgttc
     4501 tattgtgttt cctcttagca aagtgcgccc ttgtttgcac catctttctt tttcttcttt
     4561 attgagcgca accagttagt atttactttg gctcgagaca cacatgatgt gaaatctatt
     4621 tggggacaca gtaatgacaa agtatacaca ataaaaattt gaattgatgg ttattagcac
     4681 caagattaaa cattatataa aaggatccag tattttgcta ctgaaatcga taccgttgcg
     4741 gcatttcata atacctagcc ataatattct ctcttgttga aatcaagatc tactatctct
     4801 ttccaagatc actttagatt cgttaaagct tatcttataa tttctcagta catccatacc
     4861 caaaagactt ggataacgct cctggtcaac tttcgcaaca ttactttgcc ggagaaactt
     4921 cacaggcttg acatcgactg attctatccc atccccaaaa tacaaatggc atgaatctgc
     4981 taggtatgac tcggctgaac caccaatacc aagcgaattt tgatattttg tcaattttga
     5041 gtaatctact ttcatttgtc gagcatcata ttcatttatt gttgtaatag ca
//
LOCUS       DASQTF010000051         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1026943,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000051 DASQTF010000000
VERSION     DASQTF010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026943"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..440)
                     /locus_tag="VE308_01255"
     CDS             complement(162..440)
                     /locus_tag="VE308_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99626.1"
                     /translation="MSQNSNDQGAMQQTGSQWAEIIGQLFDRMTGKGASATYAFNNLV
                     IDMPKAQGPGGRDMGAAKWTINGKVTIAWEAHKTSEEAHQRASVSTVT"
     gene            799..1251
                     /locus_tag="VE308_01260"
     CDS             799..1251
                     /locus_tag="VE308_01260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZA99627.1"
                     /translation="MNAINNYSLPVPKDKLQRIDRMSSPAHVGKLRNAIDFIVAENTP
                     VLAAADGVVTFVQDGSHIGGPSIEYWHGSNFIVIQHPNSEYSRYDHLAHRSAVVRAGQ
                     YIKRGQVIARVGMTGFTYVAHLHFQVFVFTGNNIWTDFDTLSITEFLS"
     gene            1675..2178
                     /locus_tag="VE308_01265"
     CDS             1675..2178
                     /locus_tag="VE308_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99628.1"
                     /translation="MFMSSAVQKLVGFWHRYENLNLKIAFVLISLQLIHLYWLTADVV
                     IQRITGESILGVQHGSPLFIFFIIIDYVEIPALIAGLTYYALSIYKHEKGSARNALLL
                     SMLAIQVFHIFWITDEVVYDSFFGSPSGSVEIPFYAAWVAILIDYLELPVIGDLFYRT
                     IVKGERR"
     gene            2216..2587
                     /locus_tag="VE308_01270"
     CDS             2216..2587
                     /locus_tag="VE308_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99629.1"
                     /translation="MLQLLAIVVLITTTTITGAYAQDVNDTQEQKPTEEKQGGEYSGF
                     EEAQRQTEGIPNQEANPTPQAPISDLNVQQENQTTAADTTTGVAAEPTSTPGVAMLAG
                     IGVVIAAAIGAGVWYFKRGRS"
     gene            2942..3349
                     /locus_tag="VE308_01275"
     CDS             2942..3349
                     /locus_tag="VE308_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99630.1"
                     /translation="MASSSGSPPSIFEEFCQLILGLDESIRFVGIATLTGAVLAMKYR
                     ANLIPLLTQEETASSIKNSVWRVGSRRALEEKLGKTLYVVATYEKVKRATIPVGQEGQ
                     SILIISFDKETDAESIIRNKILKLIKPYGAISH"
BASE COUNT          999 a          697 c          715 g          963 t
ORIGIN      
        1 ccattgctga tcgctatttt cactgcaggc tgattttcaa agctaatcgc aagcgcacct
       61 tcttttgcag atcttagcgc tttgcttagc cagtcgctca gctgatcgtt tttgtcaata
      121 tgcaggtgac tatcgcccag catggcagct aaccaatcct tctatgttac agttgatact
      181 gatgccctct ggtgtgcctc ttcagaagtc ttatgggctt cccaagcgat cgtaaccttg
      241 ccgttaatag tccattttgc ggctcccata tcgcgcccgc caggcccctg cgctttaggc
      301 atgtctatga caaggttgtt gaatgcataa gttgcagaag cacccttgcc tgtcatccta
      361 tcaaaaagct gtccgattat ctctgcccac tgcgaaccag tttgctgcat cgcaccctga
      421 tcatttgaat tttgactcat aacgctggtt tagacaactt tggatatagc aggttttgca
      481 catttgcggt ggttttaatt ctataacaat actttataag atactataaa ctattcaata
      541 gtttcgatat acattctaat atctgaattg tctctcgcat gcatatctgt atcatggcac
      601 taagatatct cagcagaatt gtggttatgg cggtcgagtc aatttacgtt cagtactcta
      661 ggacggcatt cagactacca gagccaagct ggtatacaag acgctttttg cctaaggaat
      721 atctgaaatt ctggtcgaaa atgacgatta aacataataa ggtaaacgcg aattaagagt
      781 cgcagaaaag gcaatcacat gaatgctatt aacaattatt cgctgcctgt cccaaaggat
      841 aagttgcaga gaattgatag aatgtcttct ccagcgcatg tcgggaaatt aagaaatgca
      901 attgatttca tagttgcaga gaataccccg gtgcttgccg cagcagatgg cgttgttacg
      961 ttcgttcaag atggttcaca tattggcggc ccaagcatag agtactggca tggcagcaac
     1021 tttatagtta tacaacatcc aaatagcgaa tattctagat atgatcacct tgcgcacaga
     1081 agtgcagtag tcagggccgg gcagtatatc aagagaggac aggttattgc cagggtaggc
     1141 atgacaggat ttacatatgt ggctcatctt cacttccaag tatttgtttt cacagggaac
     1201 aacatctgga cagactttga taccctctca ataactgaat ttttgtcatg aatgtcaata
     1261 acacctttag tagaaatata gcttcaaacc atgaagcgca tatatctacc agagattgac
     1321 aatcaacgct acagcagttc aaaagcgatt gtatctgctc tacagccatc tacgtcgaag
     1381 aaaaagagtg agaatatatc gcaaaaacca tgaacatata agtttaatcg aagaacgcaa
     1441 acagttatcc agtttaagat aaccgcgtca tatctaagat ttggataagc tgctgagaac
     1501 ctggagaacc tgcatcggtt ttatctgtta tgcaaccgtg attcctttga agtaatattc
     1561 agcatagagt catcaggtat ccataactga atttttgtca tgcatacaca tacatcgaag
     1621 aaagatagta atatttttac agtcctatta attacctatt agcaagtcta gtagttgttc
     1681 atgtcctccg ctgtgcaaaa gctggtaggg ttctggcaca gatacgagaa tctgaatcta
     1741 aaaatagcat ttgtattgat ttcgctccag ctgatccacc tctattggct taccgccgat
     1801 gtggtcatac aacggataac aggagagagt atcctcggtg tacagcacgg aagcccgctt
     1861 ttcatattct tcataatcat agattatgtc gagatcccgg cacttatcgc tggcctcacc
     1921 tactatgcac ttagcatata caagcatgaa aaaggttctg caagaaatgc actcttgctg
     1981 tcaatgttag ccattcaggt atttcacata ttctggatta ctgatgaagt cgtttacgac
     2041 agcttttttg ggtcaccatc cggctcggta gaaatcccat tttacgctgc atgggtagca
     2101 atactgattg actacctaga gctgccagtc attggcgatc tcttttacag aacaattgta
     2161 aaaggcgagc ggcggtagtt gactgcataa attatttgtt gagcaataac aggcgatgtt
     2221 gcaactgctt gcaattgttg ttctgataac aactactact attactggcg cttacgcaca
     2281 ggatgtaaac gacacccagg agcaaaagcc cacggaggaa aagcagggag gggagtatag
     2341 cggttttgag gaagcacaga ggcagacaga gggaatacca aaccaagagg ccaaccctac
     2401 acctcaggca cctattagtg accttaacgt tcagcaggaa aaccagacta ccgctgcaga
     2461 tactactacg ggtgtagcag ctgaaccgac tagcacacca ggagtggcga tgcttgctgg
     2521 aatcggggtt gtcattgcag cagccatagg tgctggagtt tggtatttca agaggggcag
     2581 aagttaggtg gtgatgatag cagcctttgt gggaaatttg tatgggtctc tgagtgcagg
     2641 tgctattttt cctgtgaaag gatgggagat gcgaacgcgg ataataatac gtgcttaagt
     2701 tgtcaactag cagtgttcaa aacagtgtct tgtacctaca caactaatag aagaaagtag
     2761 ggcaaaaatg ttacttcttc attattgttt gcatatacac tctacataac ttttcatagc
     2821 tttccatata atattattaa tattctcatc atagcccgtt aagtatataa ttttattgat
     2881 aattacaacc gtaaaataaa aatagttaac tcagtatata tgatataggc tctatgcact
     2941 aatggcgtct tcatcaggct ctccgccttc aatctttgaa gaattttgtc agttaatatt
     3001 gggcctagat gagtctatta gatttgtagg catcgcaacc ttaaccggtg ccgtattagc
     3061 gatgaagtac cgagccaatt taatacccct actgactcaa gaggaaacag catcgtctat
     3121 caaaaattca gtttggagag tgggaagtag acgagctctc gaagaaaagc taggcaagac
     3181 tctctatgta gttgcgactt atgaaaaagt aaagcgagca acgattcctg taggacaaga
     3241 aggccagtct attttaatca tatcatttga taaagaaact gacgcagaat caataattcg
     3301 caataaaatt ttgaaactta ttaagccata tggtgctatt agtcactagc tgttagctag
     3361 attggttctc ctcc
//
LOCUS       DASQTF010000052         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_196111,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000052 DASQTF010000000
VERSION     DASQTF010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196111"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1019)
                     /locus_tag="VE308_01280"
     CDS             complement(213..1019)
                     /locus_tag="VE308_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZA99631.1"
                     /translation="MAKIEDALKREVDLYSWSEFHAPLRYACEGGKRIRPLILVLSAE
                     CVKSKKVEPDAYLAASAIELLHTESIIHDDIIDEENQRRGKPSFHVKYGYNSSILTAD
                     FVLGVILNISSKLKDPRIINELSNAATKMTEGEMMEIKLGKEMDITEDDYIKVLEFKT
                     ASLFEASAKIGAITGGGDDDQIHTMTAFGNLLGIAYQIHDDLIDWSNENRLFNMLVRK
                     NGKPKDFVDQMDKLYQSYASKAKNELRKISTHSEAKRHLEHLTDLTSVQF"
     gene            1370..1513
                     /locus_tag="VE308_01285"
     CDS             1370..1513
                     /locus_tag="VE308_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99632.1"
                     /translation="MRRISDNLLYGLCNKTAEPHQEGSSNYRRREEQEEIAVKIADSN
                     ISV"
     gene            1725..2603
                     /locus_tag="VE308_01290"
     CDS             1725..2603
                     /locus_tag="VE308_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZA99633.1"
                     /translation="MSQVSIGCSGWNYGDLVEKGGWVGSFYPDPQTKRLRYYSEFFPS
                     AELDATFYQKFYSKMTSGTFYGMVKATPPGFQFSVKAPETVTHTKLMDVSKGALEALQ
                     EFLEKISPLKNANKLGAILLQLPPSFSVKEFKQVEGFLNRMPSGYDYALEFRHASWQT
                     EGPWEMLKHYNVAAVMTDSPDPKLHYLSDVILTADHSFIRFHGRNKGYWYNYLYAQEE
                     LKPWTEKVNKIKKDPKAKRLRIYFNNHYGGKAVVNAIQFKEMVEGKGSSEKEKEAKQK
                     ILDALAHATAQKKLSE"
BASE COUNT          775 a          537 c          564 g          746 t
ORIGIN      
        1 atggaggagc tgcatgttgg cgtttaattg ttttgcccat agcttatcac ttcgcagagc
       61 aagtattcta tcagagagga tacgggcgtt ctatgttgag gaattgtgca gtatcatttc
      121 gtagtcagtt gaatcaaaaa attctgtcgc ttgcaagcaa gcaaacacgc aaagaaacta
      181 caaattattg gatgtttgta gtgctttttt cattagaact ggacagatgt aaggtctgtc
      241 aggtgctcca agtgtcgttt ggcttcgcta tgtgtcgata tcttgcgtag ttcgttcttt
      301 gcttttgatg catatgactg atacagcttg tccatctggt cgacaaaatc ctttggcttg
      361 ccatttttcc tgacgagcat gttaaaaagc cgattttcat tactccaatc aataaggtcg
      421 tcatgtatct ggtaggcgat ccccagcagg ttgccaaaag ccgtcatagt atgaatctga
      481 tcatcgtcgc cgcctccagt tatcgcacca atcttggctg atgcttcaaa gagcgaagcc
      541 gtcttgaatt caagcacctt aatatagtcg tcctcggtga tatccatttc cttgccaagc
      601 tttatctcca tcatctcacc ttcagtcatt ttggttgcgg cattagatag ctcgttgatg
      661 atgcggggat cttttaattt tgagctgatg tttagtatga cgcccagaac aaaatctgca
      721 gtaagtatgc tgctgttgta accatatttt acgtggaatg agggctttcc acgccgctga
      781 ttctcctcat cgataatgtc atcatggatt atagactctg tatgtagaag ctcgattgca
      841 gaagcggcca ggtatgcgtc aggttctacc tttttagact tgacgcattc tgccgacagc
      901 acaaggatta ggggcctgat gcgcttgcct ccctcacagg catagcgaag tggagcatgg
      961 aactccgacc atgaatatag gtcaacctcg cgctttaatg catcctcgat tttggccaaa
     1021 tagacagcaa acagctcagt tagagggtta gcttcgatgt ttctcctact gctcaagaac
     1081 tggctctatc cctgagacta ttttcaaact ggtgatcata ttaatttact gctctttaaa
     1141 gtaaagaagg agaaaaagta ctctgttaac ttgaacttga atccgattgg aaagcgctat
     1201 atcatcgagg agatgacatg atgatatatc gccaatagaa caccaagtgc agtcaatatc
     1261 ttttcaaaaa aacgatctcg actatatact attttccatg atgtagccaa aataaaaact
     1321 cttgagcatg atattggatc cctgaaatcg ctgccacagc cgatgtatca tgcgtagaat
     1381 ttctgataat ttgctctatg gattatgcaa taagacagca gagccacacc aagaaggaag
     1441 cagcaactac agacgaagag aagaacaaga agagatagcc gttaagattg ctgacagtaa
     1501 catttctgtt taatatggaa taccgatatt aaagaagtaa tgaatacctg cgagcgtata
     1561 tctcgagtat aaaacagctg ttcagtacaa actaaggctg cagagacgac atttgagttt
     1621 ttatgcaagt caaaatgata ataacaattg tattcttttt tctattttca agaatcgagc
     1681 atagtgacaa gcaaatatct gagatgttcc tagcatatca tatcatgtca caagtatcca
     1741 taggttgctc tggctggaat tatggcgatc tggttgaaaa agggggctgg gtaggctctt
     1801 tctatcctga tcctcagact aagcgtctgc gttactattc tgaatttttc ccttcggctg
     1861 agctcgatgc tacattttat cagaagttct attcaaagat gactagtggt acattctatg
     1921 gcatggtaaa agctacacct ccaggatttc aattctctgt caaggctcct gagaccgtga
     1981 cacatactaa gctcatggat gtgtcaaaag gtgcactgga agcgctccaa gagttcctcg
     2041 aaaagatttc tccgctaaaa aatgcaaaca aacttggtgc aatacttctg caactaccac
     2101 cgagtttctc agttaaggag ttcaagcaag tcgaaggatt cctcaataga atgccatcag
     2161 gctacgatta cgcgctggag ttccgccatg catcatggca gactgaggga ccttgggaaa
     2221 tgctaaagca ctataatgtt gcagctgtaa tgacagattc gccggatccg aagctgcatt
     2281 atctatcaga tgtcatattg actgcagacc attcgtttat tagatttcat ggcagaaata
     2341 agggttattg gtataactat ctttatgcac aagaagaact aaagccttgg actgaaaagg
     2401 tcaacaaaat aaaaaaagat cccaaggcaa aaagattgcg aatttacttc aacaaccact
     2461 atggcggcaa ggctgttgtc aatgccatcc aattcaaaga gatggtcgaa ggaaagggat
     2521 cttcagaaaa ggaaaaggag gcaaagcaga aaatattgga tgctttagct catgcaactg
     2581 ctcaaaagaa actctctgag taagtgcgcg tgcatctagc ct
//
LOCUS       DASQTF010000053         8944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_749364,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000053 DASQTF010000000
VERSION     DASQTF010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749364"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VE308_01295"
     CDS             <1..740
                     /locus_tag="VE308_01295"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HZA99634.1"
                     /translation="AIANSNSRSVTAVDLQVRQLRDKSPNNMGNILLVELIIDTKDAM
                     GANAINTMCEAIAPHVAEITGGEVVLKILSNYSTRRMVRCRATFDKKELGGSQIVKRI
                     LYAYALAYSDAYRAVTHNKGVMNGIDAVALATGQDFRAIEAGAHAYAARNGTYRSLTS
                     WRRTKEGDLAGEIELPLAVGVVGGVVNAHPTAKFALQILGVKSAKDLAMVIAAAGLAQ
                     NLAAIRALSSEGIQAGHMRLHARKFSK"
     gene            787..1764
                     /gene="hisG"
                     /locus_tag="VE308_01300"
     CDS             787..1764
                     /gene="hisG"
                     /locus_tag="VE308_01300"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZA99635.1"
                     /translation="MPTVKFAIPKGSIEEVTFNLLEQAWQHVSGRGRTYRVKLGDPAI
                     DVKILRPQEIPTYVQEGFYDVGITGRDWILEAKADVEVLLDLEIGRVKQVIAVPTSFP
                     FDNLSDLIVDFAKNNKTLRISSEYLTTTSAHIKANAMYKKYYGNADPMIITPWMRLGE
                     NKKVEIFLSFGATEAKPPEDVDAIFDITETGTTLAQNKLKIIDTVAESTAVLMANKKS
                     LKDPAKREKIADMIALLRGVVEGRKKLHIFVNVRKEKLERLLEELPALKRPTVSPLSE
                     EGWYGVNTVIDKNEFIMIVPKLRKLAQGLVVLEPRQILPLDEINLDGYS"
     gene            1764..3038
                     /gene="hisD"
                     /locus_tag="VE308_01305"
     CDS             1764..3038
                     /gene="hisD"
                     /locus_tag="VE308_01305"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZA99636.1"
                     /translation="MKIIRVTDPGSEASQLRRATLIQESFLEDAKAIMRDVAEHGDPA
                     VLNYTSKFDGARLDSLRVSEHEMKQAYAQVTKDQIMAVKLMKQRLVKSELAILKRLKG
                     VVVSSDGVRIDRMVKPVTSVGCYIPGGRARYPSTVVMCTVPAKVASVKRIVALSPPRR
                     DGTIDPLTLVAADICRVDEFYKVGGAQGIAALTYGTQSIRPVSKIVGPGGMFVTAAKL
                     VASTTVSTDMVAGPTELLIYADATADPQLIAVDLISQAEHGTDTVCGLVTTSDKLASE
                     VQKRVESIVKKITRSDIVKKSLENNGFIVICRDESSCIEFVNEFAPEHLEIICKYADA
                     IAKKIDSAGLVLIGQYTPSSASDYSLGSNHVLPTLGFGKSRASLSVLDFVKIVNKVKV
                     NKSGLAKVDRSIREITSAEGLLNHYEAVKARG"
     gene            3039..4109
                     /gene="hisC"
                     /locus_tag="VE308_01310"
     CDS             3039..4109
                     /gene="hisC"
                     /locus_tag="VE308_01310"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZA99637.1"
                     /translation="MKKKNSWLSDNIKRIAWLDYYSKPEKVEGAIKLDSNENFALDRD
                     FVAGVAAEAAKQVDLREYPLDQMDELYARLAKYAGVSVKCVAVGSGSDQIIELLLSTL
                     GGKYATVFSPTFSYFVNRCKLHGIKVYQVPLELDFTLDKKAFMKTAKRSDLIYICSPN
                     NPTGNQLDRQEIIEVVDSLEDRLVLIDEAYVDFADYSLSSEAIKRNNVIVLRTLSKAF
                     GLAGARVGYIVSNRKFVKVFRSIIQSPYPISTLSLAIASGVLADVDLVKQTIKSVRSE
                     RERVFARLSKIDGIKIFRSDANFVFLETGKNYHAISKTLNSNGIVVKLLGNITNHRGC
                     MRITIGTREMNDKYLKCIEQII"
     gene            4088..5107
                     /locus_tag="VE308_01315"
     CDS             4088..5107
                     /locus_tag="VE308_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZA99638.1"
                     /translation="MHRTDNLMYDCVLFDIDGVLVDIRKSYNAAIKETVEYMLKFITG
                     SSFRTLVTDQIILKFRQSGGFNNDMDTSYAITLAILANPPKNILQGRKFLAKVAENSD
                     ESGYISVEKFLANYDIDKWKKILTYPAPVKDSMLARVFDEIFYGPNLFRKQDHLKPKY
                     RTTGRPLIKNDRLAVSAKAMKKLNKMFKGNLAIVSGRSRLAAEYSLQPIIKYFNSNAC
                     VFLEDKKRKYAKPNPYAVKHAMKVMGARTAVYVGDSAEDLLMARRAEKETDAKIAFVG
                     IYGNSSEPDKTIDKFKQEGVEAIIRSVNQLPNIINKVHICSLKYTWLLPEEKRGLKGS
                     QRRRR"
     gene            5050..5646
                     /locus_tag="VE308_01320"
     CDS             5050..5646
                     /locus_tag="VE308_01320"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HZA99639.1"
                     /translation="MASAGRKARIERVTKETAVNVNVNLDGTGKTRIKTRLSFIDHLI
                     MSIGKHAMIDITLNGESNDGIVHHLVEDVAIALAQSIDKALGDRTRITRFGYALIPMD
                     EALAYVSIDLVKRQYYNVQLKLTRDSIEGVPREDLEHFARSLVQNLNACTHMVVYYGE
                     NDHHKVEAALKAFAVALRMATNIDSERKGAPSAKGAMQ"
     gene            5643..6266
                     /gene="hisH"
                     /locus_tag="VE308_01325"
     CDS             5643..6266
                     /gene="hisH"
                     /locus_tag="VE308_01325"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZA99640.1"
                     /translation="MTKIAIFDYGAGNLFSLKSALERNGAENVDIIYDLKGLTKFDGL
                     VLPGVGNFDPAIRSIEGSASFLDKAIDNCMPLLGICLGMEILFNKSEEGRLEGLKILE
                     GEVVILPRGKVKIPHMGWNNLQIQCESKLLKGVKDQSWVYFVHSYKTVPKDRKLVVAT
                     SDYGVSIPAMIEKDNLIGVQFHPEKSGDVGALMIKNFIGMCKKEEAK"
     gene            6263..6961
                     /gene="hisA"
                     /locus_tag="VE308_01330"
     CDS             6263..6961
                     /gene="hisA"
                     /locus_tag="VE308_01330"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZA99641.1"
                     /translation="MKVIAAVDIMGGSVVRLVKGNPANKVEYSNDPVETAKKWETVGA
                     DMLHVVDLDAAFSTGNNSELIAKISEAVRIPLQIGGGVRSLEKAEEMLSMASRVVIGT
                     MAYREPEVIRKLVKKNAGRVAISVDQIDGMVMVKGWKESTGTSVAEAISQFLAIGVDE
                     FLLTSIDRDGTLQGPDLKTLADAAMTARIIASGGISSIEDVIRIRSAGCSSVIIGKAM
                     YDGKVSIEKVKAVA"
     gene            6961..7767
                     /gene="hisF"
                     /locus_tag="VE308_01335"
     CDS             6961..7767
                     /gene="hisF"
                     /locus_tag="VE308_01335"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZA99642.1"
                     /translation="MTLAKRIIPCLDVDNGRVVKGVHFAEVKDAGDPVELGKKYREQG
                     ADELVFLDITASQQGRETMASIVRKVAGVIDIPFTVGGGIKKLEDARDILLSGADKVS
                     INTAAVKNPELIMDLMSIFGKQCIVVAIDAKRNYTVEKDKMMFKDNDGRKFWFEVRIY
                     GGKEGTGLDAIKWAKEIEQRGAGEILLTSIDADGTENGYDIELTKSICKSVPVPVIAS
                     GGCGKARHMLDVFEKTDVDAALAASIFHYEKSTVDKVKKYLKRNGIKIRI"
     gene            7805..8134
                     /locus_tag="VE308_01340"
     CDS             7805..8134
                     /locus_tag="VE308_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99643.1"
                     /translation="MFGTSPGRITFENHLGKLKEPVRPIMADLRKFVISLGSNVIEEV
                     RPHRIVYSKTLNFRTFLDMEATAGDSLILSIRYGRGAPPVSVMVMTIEDAERAKKQIA
                     EAYQKIQ"
     gene            complement(8135..8440)
                     /locus_tag="VE308_01345"
     CDS             complement(8135..8440)
                     /locus_tag="VE308_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZA99644.1"
                     /translation="MQVSDTKKKTRAHVFIRGKVQGVYFRQNTKQVATKHRVTGWVCN
                     LLDGRVEAVFEGDKIDVNEVIEWCHIGPPNSRVDDVDVRFEKYIGEFASFTINNNNN"
     gene            complement(8505..>8944)
                     /locus_tag="VE308_01350"
     CDS             complement(8505..>8944)
                     /locus_tag="VE308_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99645.1"
                     /translation="ESSILVSKIVNTKEYNEHKIVIVDAGYHILLDAALLKQEYPQEV
                     VPDSKNKMTPLATVKNTHLAGRLCDTYDVFPLSKVSDLTESEVGRYVSFYNVGAYSVV
                     FNMPFHCQTKPPIVMKDSDGKFRLVRKGTTYDQLFREEGGELE"
BASE COUNT         2672 a         1839 c         2244 g         2189 t
ORIGIN      
        1 ttgccatcgc caattctaat agcaggtctg tgactgcggt cgacttacaa gtgcgacagc
       61 tgcgcgacaa aagtccaaat aatatgggca atatcctgct ggtagagctg atcattgaca
      121 ccaaggacgc catgggtgca aatgcaataa atacaatgtg cgaagcaata gcaccgcatg
      181 tagccgaaat tactggtggc gaagttgtgt taaagatatt atccaattat agtaccagaa
      241 ggatggtcag atgcagggcc acctttgaca agaaagagct tggcggcagc caaattgtca
      301 agcggatact ctatgcttat gcgcttgctt attctgacgc ttaccgtgca gttactcaca
      361 acaagggtgt catgaacggt attgatgcag ttgcgcttgc caccggtcag gactttcggg
      421 cgattgaagc tggtgcgcat gcatacgctg caaggaatgg aacctatcgg tcgctgacta
      481 gctggcgcag gacaaaggaa ggcgacttgg ctggtgaaat cgagttacct ttggcagtag
      541 gagttgttgg aggagtagtc aatgcccatc ctactgccaa gtttgcgctt caaattttgg
      601 gcgtcaaaag cgcaaaggat ctagcaatgg tgatagctgc tgcaggtctt gcacagaatc
      661 tggctgcaat aagagcgcta tcctcagaag gtattcaagc tggccacatg aggttgcatg
      721 caaggaaatt cagtaagtag atatatatcc agtaaaagca cttttctcaa actgtcacat
      781 catataatgc ctactgtaaa atttgcgatc ccaaaaggga gcattgagga ggttacgttc
      841 aatctactag agcaggcatg gcaacatgtg agcggccggg gccggactta ccgagtgaaa
      901 ttgggtgatc ctgctattga tgtcaagatt ctgaggccac aagagatccc cacatatgtt
      961 caggaaggct tctatgatgt gggcatcact ggcagggact ggatacttga ggcaaaggca
     1021 gacgttgaag tgttgctaga ccttgagatt ggacgcgtca aacaggtgat cgcagtcccc
     1081 accagttttc cattcgataa cctgagcgac ctgatagtcg actttgccaa gaacaacaag
     1141 acgttaagaa tatcctcaga atacctcacc acgacttctg ctcatatcaa ggcaaacgcc
     1201 atgtacaaga agtactatgg caacgccgac cctatgataa ttacaccatg gatgaggtta
     1261 ggtgaaaata aaaaggtcga gatctttctc tcatttggtg ctacagaggc caagccgcca
     1321 gaagatgtag acgccatttt cgatattact gagactggca ccacgcttgc tcaaaacaaa
     1381 ctcaaaataa tagacactgt agccgaatct actgcagtgc tgatggcaaa taaaaagtcc
     1441 ctcaaggatc cggccaagcg cgaaaaaata gccgacatga tagcgctcct caggggtgtc
     1501 gtagaggggc ggaagaagct ccacatattt gtcaatgtca ggaaggagaa gttggaaaga
     1561 ctattagagg aactgcctgc gcttaaaaga cccactgtca gcccactatc tgaggaaggc
     1621 tggtacggcg tcaacaccgt catcgacaag aatgagttta tcatgatagt gccaaagctg
     1681 agaaagctgg cgcaggggct agttgtattg gagccaaggc aaatactacc acttgatgag
     1741 ataaacttgg acggatattc ctgatgaaga taatccgcgt cactgaccca ggtagcgaag
     1801 cttcgcagct tcgcagggca actttaatcc aagagtcatt cttggaagac gcaaaggcaa
     1861 taatgaggga tgtcgcagag catggcgacc ctgcagttct caattacacg tcaaagtttg
     1921 acggcgcaag gcttgattca ctacgagtta gcgaacatga gatgaagcaa gcatatgcgc
     1981 aagtcacaaa ggatcagatt atggctgtca agttgatgaa acagaggctt gtcaagagcg
     2041 agctggcaat ccttaagcgg ctaaaaggag tggtggtgtc atctgatggt gttaggatag
     2101 atcgcatggt caagccggtt acaagcgttg ggtgctatat tccaggcggc agggctcgat
     2161 atccaagcac cgtcgtcatg tgcacggtac ctgccaaggt cgctagtgtg aaaaggattg
     2221 tggcgctatc tcctccaaga agggacggca ccattgaccc attaacattg gtggctgcag
     2281 acatttgcag agtcgacgaa ttctacaagg taggcggcgc gcagggaata gctgccctta
     2341 cctatggcac acaatcgatt aggcctgtca gcaagatagt gggtcctggc gggatgtttg
     2401 ttaccgctgc aaagctagtc gcttccacta cggtgtcaac agacatggta gccggcccga
     2461 ctgagcttct aatatatgcc gatgcaactg ctgacccaca gcttattgct gtagatctga
     2521 tctcgcaggc tgagcacggc actgacactg tatgtggcct tgtgactaca tctgacaagc
     2581 tggcatcaga agtgcagaag cgagtagagt cgatagttaa aaaaattaca cggtcagata
     2641 ttgtgaaaaa gagcctagaa aacaatggct ttattgtcat ttgtagggat gaaagctctt
     2701 gcattgaatt tgtaaatgag tttgcacctg agcacttaga aataatatgt aaatatgctg
     2761 acgctattgc caagaagatt gattcagctg gacttgtgct gattggacag tatacaccat
     2821 catctgcaag cgactacagc ctcggttcaa atcacgtgct tcctaccctt ggatttggca
     2881 agtcccgcgc atcactttca gtgcttgact ttgtcaaaat agtaaacaag gtgaaagtaa
     2941 acaagtcagg attggcaaag gtggatagat ccattaggga aattacatct gcagagggac
     3001 tcctgaacca ctatgaagca gtgaaggcga gaggatgaat gaaaaaaaag aatagctggc
     3061 tcagtgataa catcaagcgg attgcttggc tcgactacta ttcaaaaccg gaaaaagtgg
     3121 aaggtgcaat taagctagac tcaaatgaaa atttcgcgct tgatagggat tttgtcgcag
     3181 gcgtagcagc tgaagctgca aagcaggtag atctcagaga ataccctctt gaccagatgg
     3241 atgagctcta tgctcggctt gccaagtatg ccggtgtcag cgtaaagtgt gtagctgttg
     3301 gcagcgggtc tgaccaaata attgaactat tgttgtcaac gcttggaggt aaatacgcaa
     3361 cagtgttttc tcccacattt tcatattttg ttaatcggtg caagcttcac ggtataaagg
     3421 tgtatcaagt tccgctagag ctcgatttta cgcttgacaa aaaggcgttt atgaagactg
     3481 caaagaggtc cgaccttatt tacatctgct cgccaaacaa tcctactggc aaccagcttg
     3541 atagacagga aataattgaa gtcgtcgatt cgcttgagga cagacttgtg ttgatcgacg
     3601 aggcatatgt agactttgca gattactcgc tgtcaagcga agcaataaag cgtaataatg
     3661 taatagtgct tcgtacgcta tcaaaggcat ttgggcttgc cggtgcaagg gttggctaca
     3721 tagtgtcaaa tagaaaattt gtgaaggtat tccgatctat catccagtcg ccctatccta
     3781 taagcacact ttcgcttgca attgcatcag gtgtcctcgc agatgtggat ctggtcaagc
     3841 agacaatcaa atctgtgagg agtgaaagag agagagtttt tgccagattg tcaaaaattg
     3901 atggcatcaa aattttcaga tcagatgcta attttgtctt cctcgagact ggcaagaact
     3961 accacgcaat ttcaaaaact ctcaacagca atggaatcgt agtaaagctg ctgggcaata
     4021 tcactaacca cagaggctgc atgaggatca caataggtac acgggagatg aatgacaagt
     4081 atcttaaatg catagaacag ataatttgat gtatgattgt gtgctctttg acatcgacgg
     4141 cgtgttagtt gacattcgca agtcctataa tgctgccatc aaagagactg tggaatacat
     4201 gctcaaattt attactggca gttcttttcg aacactagtc accgaccaga tcattttgaa
     4261 gtttaggcag agtggtggct ttaacaatga catggatact agttatgcta taacccttgc
     4321 aatccttgcc aaccctccaa agaacatctt acagggaagg aagtttcttg ccaaggtagc
     4381 agagaattct gacgagagcg gctatatctc ggtggaaaag ttccttgcta actatgacat
     4441 tgacaagtgg aaaaaaatac tgacctatcc agcaccggtc aaagatagca tgcttgcacg
     4501 ggtttttgat gagatatttt atggaccgaa cctgttcaga aagcaggatc atttgaagcc
     4561 caaatatcgg acgacaggca ggccgctgat aaagaacgat aggctcgcag taagtgccaa
     4621 agcgatgaaa aagctcaaca aaatgttcaa aggcaatctt gcaatagtct caggcagaag
     4681 caggctcgct gcagaatatt cactccagcc cataataaaa tatttcaact ctaatgcctg
     4741 cgtcttttta gaggacaaaa agcgcaagta tgccaaacca aacccttatg cagtcaagca
     4801 tgcgatgaaa gtgatgggtg caagaactgc agtttatgtg ggtgactctg cagaagatct
     4861 gcttatggca aggcgagctg agaaagagac agacgcaaaa attgcatttg tgggcatcta
     4921 tggcaacagc tctgagccgg acaaaaccat agacaagttc aaacaggagg gcgttgaggc
     4981 aattataagg agcgtaaacc aactaccgaa cataataaat aaagtacaca tctgtagctt
     5041 aaaatacact tggcttctgc cggaagaaaa gcgaggattg aaagggtcac aaaggagacg
     5101 gcggtaaatg ttaacgttaa tctcgacggc actggtaaga cacgcatcaa gacacgtctt
     5161 tctttcatcg accacctgat catgtcaatt ggcaagcatg ccatgatcga tattaccctc
     5221 aatggcgaat caaatgatgg catcgtacac cacttggtag aagatgttgc tattgcattg
     5281 gcgcagagca tagacaaagc gctgggtgat aggacaagga tcacacggtt tggttatgca
     5341 cttatcccga tggacgaagc gttggcgtac gtgtcgatcg acttagtcaa gcggcaatac
     5401 tacaacgtgc agctgaaact tacccgcgat agcattgaag gagtaccaag agaagatctt
     5461 gagcactttg caagatcatt ggtgcagaac ctaaatgcgt gcactcatat ggtagtctac
     5521 tacggtgaaa atgatcatca taaggttgaa gcagcactca aggcgtttgc agtggcactt
     5581 aggatggcca ctaacattga cagcgaaagg aagggcgcgc caagtgccaa gggggctatg
     5641 caatgacaaa aatagcgatt tttgactatg gcgcaggcaa cctcttcagc ctcaaatctg
     5701 cgcttgaacg caacggagca gaaaatgttg acataatcta cgatctaaaa ggactgacaa
     5761 agtttgacgg gttggtgctt cccggtgtcg gcaactttga tcctgcaatc agatcaatag
     5821 agggtagtgc aagctttctt gataaagcta ttgacaattg catgccgctg ttgggaatct
     5881 gccttggcat ggagatactc tttaacaaga gcgaggaagg gagactggaa gggttgaaga
     5941 tattagaggg cgaggtcgta atacttccaa ggggaaaggt caagattcct cacatgggat
     6001 ggaacaacct ccaaatacaa tgcgagagca agctgctaaa aggagtcaag gatcagtcgt
     6061 gggtatattt tgtgcattct tacaagactg taccaaagga tcgtaagtta gtagtggcga
     6121 cttctgacta tggtgtcagc atcccagcga tgattgaaaa ggataatctg atcggtgtcc
     6181 agtttcatcc agaaaagtcg ggcgatgttg gtgcacttat gataaagaac tttatcggaa
     6241 tgtgcaaaaa agaagaagcg aaatgaaggt catagccgca gttgacatca tgggtggaag
     6301 cgtagtcagg cttgtcaagg gaaatcctgc taacaaggta gaatatagca atgatcctgt
     6361 tgaaactgca aaaaagtggg agactgtcgg cgcagacatg ctgcatgtag ttgacctcga
     6421 tgcagcattt tctactggca ataattctga gctaattgca aaaatatcag aagcagtcag
     6481 gatccccctc cagattggag gcggcgttag atcactggaa aaagctgaag agatgctgtc
     6541 aatggcatca agggttgtta ttggcactat ggcctataga gaaccggagg tcataagaaa
     6601 gcttgtcaag aagaatgcag gcagagtagc aatttcagta gatcagatag acggaatggt
     6661 tatggtgaaa gggtggaaag agtctactgg aacatcagtt gcagaggcaa tatcgcagtt
     6721 tcttgcaata ggggtcgatg aatttttgct gacaagcatc gatcgcgatg ggacattgca
     6781 aggtcctgat ctcaaaacat tggcagatgc cgctatgact gcaagaataa ttgcaagcgg
     6841 tgggatctca agtatagaag atgtgataag gatcagaagc gccggctgta gctctgtcat
     6901 aattggaaaa gctatgtacg acggcaaagt gagcatcgaa aaggtgaagg cagtagcata
     6961 atgacacttg caaaaagaat aatcccttgt cttgacgtcg acaacggcag ggtcgtcaag
     7021 ggagtgcatt ttgcagaggt caaggacgcc ggtgacccag tcgagcttgg caaaaaatat
     7081 cgcgagcaag gagctgacga gctagtgttc cttgacatca ccgcgtcgca gcagggaagg
     7141 gaaacaatgg caagcattgt gagaaaggtg gcaggagtca ttgacatacc attcacagtt
     7201 ggcggcggca tcaaaaaact tgaagacgca cgcgacatat tactttctgg cgcagacaag
     7261 gtctcgatca acactgccgc agtcaagaac cctgaattaa taatggatct gatgtcgatc
     7321 tttggcaagc agtgcattgt agtagcaata gatgctaaga gaaattacac tgttgaaaaa
     7381 gacaagatga tgttcaagga taatgatgga aggaaatttt ggtttgaggt cagaatctat
     7441 ggcggcaaag aaggaacagg acttgatgct atcaagtggg ccaaggaaat cgagcagcgt
     7501 ggggccggcg aaatattgct gacaagtatt gatgctgacg gcactgagaa tggctatgac
     7561 atcgagctga caaagtccat atgcaaatcc gtgcctgtgc ctgtaattgc ctctggcggc
     7621 tgcggaaagg cacgccacat gctcgacgtg tttgaaaaga cagatgttga cgccgcgctg
     7681 gcggcatcga tattccacta cgaaaaatca acagtcgata aggtaaagaa atacctaaag
     7741 agaaatggca tcaagatcag gatataataa tataaaagag atgtgacctt acagtcatta
     7801 tattatgttt ggcactagtc ctggccgcat tacatttgaa aaccacttgg gaaagttaaa
     7861 agagccagtt agaccaataa tggctgactt acgaaaattt gtgatatcgc ttggcagcaa
     7921 tgtgatagaa gaggtaaggc ctcacagaat agtgtattcc aagaccctga actttagaac
     7981 atttcttgac atggaggcaa ctgctggtga ctctttgata ttgtctataa ggtatggccg
     8041 aggtgcacca cctgtatctg taatggtcat gactatcgag gatgcagagc gtgcaaagaa
     8101 gcagatagcc gaagcatacc agaaaattca gtaattaatt gttgttgttg ttgatagtaa
     8161 agctggcaaa ctcgccaatg tacttctcaa atcttacatc cacgtcatcc actctggagt
     8221 ttggtgggcc aatgtgacac cactctatta cttcgttgac atcgattttg tcgccttcaa
     8281 agactgcttc tacacgacca tccaacaagt tgcataccca gcccgtgacc ctatgtttag
     8341 tcgctacttg ttttgtattt tgcctgaaat aaactccctg caccttgcct ctaataaata
     8401 catgtgcgcg agtctttttc ttggtatcgc tcacttgcac tcactgcaat tgttgattta
     8461 aaaaatgttc ccgccagcaa aaatatttgc ggcagcggaa actattactc tagctctccg
     8521 ccttcttcgc ggaacaattg atcgtatgta gtccccttcc ttaccagcct gaacttgccg
     8581 tcgctatcct tcatcactat gggaggctta gtctgacagt ggaatggcat gttgaatact
     8641 acagagtatg cgcctacgtt atagaatgat acataccgtc caacttccga ctcggttagg
     8701 tcagacactt tcgaaagtgg gaagacgtca tatgtatcgc agagtcttcc tgctagatga
     8761 gtattcttta cggtagcaag tggggtcatc ttatttttgc tgtctggtac tacttcttgt
     8821 gggtactctt gctttagtag tgcagcatca agtaggatat ggtaacctgc gtccacaatc
     8881 actatcttgt gctcattata ttccttggta ttcactatct ttgaaacaag gattgacgat
     8941 tctg
//
LOCUS       DASQTF010000054         3518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1122041,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000054 DASQTF010000000
VERSION     DASQTF010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122041"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VE308_01355"
     CDS             complement(<1..193)
                     /locus_tag="VE308_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99646.1"
                     /translation="MVNNKSKAFEEGKRAAWISVWTLLGIGVAEVGISTTTGSLTLFA
                     DGLDSMADALVSFIVWFGIT"
     gene            1013..1411
                     /locus_tag="VE308_01360"
     CDS             1013..1411
                     /locus_tag="VE308_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99647.1"
                     /translation="MALGELLGEARGKVAGLRALGDGIIEVSLQGKGTLLGSEIEDVT
                     TFGSAMRPNGTAYGQGNSIQMSTEGMAEWRGSGVGKPTGPGVWKYAYGDVYTIATSEK
                     LQRLLNVYTAGEYENDANGNYYWKLWEWKF"
     gene            complement(1549..2631)
                     /gene="amrS"
                     /locus_tag="VE308_01365"
     CDS             complement(1549..2631)
                     /gene="amrS"
                     /locus_tag="VE308_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZA99648.1"
                     /translation="MVILQTSLPGKDAELYTRLPNGRIKCTACARLCEIPEGKVGLCG
                     IRGVANDKLRLFVYGRVIAGQIDPIEKKPVTHYRPGTDIFSISTTGCNWLCQYCQNYD
                     ISQRRKVEGTEMTPEQVVENAAYYGAHGIAYTYNEPSIFIEFAKDCGIEAHKRGIFNI
                     FVSNGYDTPESVNMMSQFLDCITVDFKGSGKQDFVRRYIGIPSADPIFQTLKEIRDKT
                     KIHVEITDLIVPQVGDDLETARKLCRFVHDELGPDTPIHFLRFHPDYKMMQFDNTPIE
                     TLEKHYEVAKQEGLTYAYLGNVPGHPLEDTYCPECKGVVIGRYGFSIDEWNLDANNCC
                     KNCGCSIPIIGKLHKVRKKNHFQFIH"
     gene            complement(2703..3227)
                     /locus_tag="VE308_01370"
     CDS             complement(2703..3227)
                     /locus_tag="VE308_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99649.1"
                     /translation="MADSFIDSAIIAKSQESVRPDQPLTLSLSFRIQGSIREAFSQKN
                     WERAYNKHDNGFIMKIEIDLKSGRRRIALIKLVRKAALFWTRNPKIPYRIWVSVVKDD
                     TPLYPLTEEEAKSLLFDVNKVIELRGSDLNSGTHKLYADIKVSWGKHVYTQPNEIKGR
                     SNHIELLRVAAPDI"
     gene            3300..>3518
                     /locus_tag="VE308_01375"
     CDS             3300..>3518
                     /locus_tag="VE308_01375"
                     /inference="COORDINATES: protein motif:HMM:NF025040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoL/Rpb11 RNA polymerase subunit family
                     protein"
                     /protein_id="HZA99650.1"
                     /translation="MADVKDNEIELKIREEDISVLYILQHELLKEKSIDFAGVIQKHP
                     LTNDYQLRVATKRKDPMEVIQDASTSAQE"
BASE COUNT          953 a          737 c          757 g         1071 t
ORIGIN      
        1 tggtgatacc aaaccatact atgaatgaga caagcgcatc agccatagag tctaaaccat
       61 ctgcaaacag tgtcaggcta cctgttgtag tagaaatgcc aacttcagct acccctatac
      121 ccagtagtgt ccatacagat atccacgccg cacgctttcc ttcttcgaaa gctttcgact
      181 tattattaac caagaaatat acgagtgtta ttagattata gctgcctgtt aattaaccat
      241 tatgcttgct tctttgcttc tctttagctg tcctattatt taatgtgcga aagtaacagc
      301 tactacatac aggaatagaa gagcttcttc tggcacattt attatatagt ctgctccgac
      361 tctgttacaa attgcataat tgtagtatgc tatactgcgc cattctagca tgtccaatgg
      421 atggcgggct agtggtggaa gaggaggaga agctatcgtg tcagcttatt ctttcatcaa
      481 gaaattttga catgaaatga tacttatagt ttagtaatct ctctttgtat agagtctagc
      541 accataaaaa gtgccatctg attcattctc gcgctttcca ttctcttcag tgttgaagat
      601 atcgggaata aaggctacat tcattgctga ttgaaaaaat accgctcata attctagcca
      661 taaaacgaag accggtcttt catttttacg tctgaatttt tccaacaatg cctttttgtt
      721 atgtatatga ggcggtgcta ctgcttcttc tgtgcagatg ctgctaataa ttatggagcc
      781 gtatcactgt agggtatcac tgtagggcga tattgttaga aaacaagggc ccgttgggct
      841 tagaaaaaat attcataaaa tagactctca tgaatcagga ttgcacagta ggagaactgg
      901 gcctgcatta tttcatacgc atatccgtca tgaactacct ccaccattat ctagccttgg
      961 ggtgtaaaaa attgcataat gcttatctac actatttgca gttagcgctt caatggcatt
     1021 aggtgaactg cttggagagg cacgtggaaa ggttgccgga ttaagagcgt tgggtgacgg
     1081 tataatcgaa gtctcacttc aaggcaaggg cacgcttctt ggttctgaaa tagaggacgt
     1141 aacgacattt ggatcagcaa tgagaccaaa tggtacagca tatggtcaag ggaattcaat
     1201 tcagatgtct actgaaggta tggcagaatg gcgcggctct ggggttggca aacctacagg
     1261 gcccggtgtg tggaaatatg cgtatggcga tgtttataca atcgctacat ctgagaaatt
     1321 gcagagattg ctcaatgttt acaccgcagg cgaatatgaa aatgatgcta acggaaatta
     1381 ctactggaaa ctctgggaat ggaagttctg acattctcaa agtgctgatt acaagaaggc
     1441 gcctttcatt ccaatctctg tgtgcatatc agtgcttttt tggtgtagga gacggtgctt
     1501 cctccatttc agtgataaga ataaagaagc tctgaagggc ttcgtaatct aatgaataaa
     1561 ctggaagtgg ttcttctttc tcactttgtg aagcttaccg ataattggta ttgagcagcc
     1621 acagttcttg caacagttgt tagcgtcaag attccactcg tcaatgctaa agccgtagcg
     1681 ccctatcact acgcccttgc attctgggca gtatgtatct tccaaaggat gacctggaac
     1741 atttccaaga tatgcgtagg tcaagccctc ttgctttgcc acttcatagt gtttttcaag
     1801 tgtttcaata ggtgtattgt caaactgcat cattttgtag tcagggtgga accgcagaaa
     1861 atgtattgga gtatcaggcc caagctcgtc atgcacgaat cggcaaagct ttcttgctgt
     1921 ttctaagtcg tcgcctacct gtggtactat aaggtctgtg atctcgacgt ggatctttgt
     1981 cttgtctcga atttctttga gtgtctggaa gatcgggtca gcgcttggta tgcctatgta
     2041 gcgtctgaca aaatcttgtt tgccgcttcc cttaaagtcg actgtgatgc agtcaaggaa
     2101 ttggctcatc atattgacag attcaggagt atcatagccg tttgatacaa agatattgaa
     2161 tattccgcgc ttgtgagctt ctataccgca gtcttttgca aactctatga aaattgaagg
     2221 ctcattgtag gtgtaagcaa tgccatgagc gccatagtag gcagcatttt ctacaacctg
     2281 ttcaggcgtc atttccgtac cttccacctt gcgcctctgc gaaatatcgt aattctggca
     2341 atactgacaa agccagttac aacctgtagt agaaattgaa aatatgtcag tgccaggtct
     2401 gtagtgagta acaggctttt tctctattgg gtctatctgg ccggcgatca ctctcccata
     2461 gacaaacaat cgcaacttgt catttgcgac tcctctgatg ccacagaggc ctaccttacc
     2521 ttctggtatc tcgcacagcc ttgcgcatgc cgtgcacttt atcctaccgt ttggcaatct
     2581 tgtgtatagc tcggcatctt tgcctggtaa actcgtctgc aatatgacca tgttagcgcg
     2641 ttttaatctt ataaatcatc gttcaatccc gccggccgtc atctctatac atctatagag
     2701 tgctatatat caggggcagc aacgcgcaac aattctatgt ggtttgacct gcctttaatt
     2761 tcatttggct gtgtgtatac atgtttgccc catgacacct tgatatctgc ataaagcttg
     2821 tgggtaccag aattgagatc gctgcctctc agttcaataa ccttattgac atcaaacaac
     2881 agggattttg cctcttcttc agttaatggg tataatggtg tatcatcctt tactacggaa
     2941 acccatatcc tatatggtat cttggggttg cgtgtccaga acagagcggc ctttcttaca
     3001 agcttgatca gcgcgattct tcttctgcca gatttaagat caatttcaat cttcataata
     3061 aagccgttgt catgcttgtt gtaagcacgc tcccagttct tttgagaaaa tgcctccctt
     3121 atggatcctt gtattcggaa gcttaggctt agagtaagtg gttgatcagg cctgacgctt
     3181 tcctgtgatt tggcaatgat ggcgctatcg ataaaactgt ctgccatttg gcagaaaagt
     3241 atcatttata tcttcaataa gtagttgttg ttgtctgtat aacggatatg tttgtagaaa
     3301 tggcagatgt caaagataac gaaattgagc taaaaatccg tgaagaagat atctctgttt
     3361 tgtatatcct tcaacatgaa ttactgaagg aaaagagcat agactttgcc ggcgttatcc
     3421 aaaagcaccc actcacaaat gactatcaat tgagagtagc gacaaagaga aaagacccaa
     3481 tggaagtcat acaggatgca tcaacgtctg cacaggaa
//
LOCUS       DASQTF010000055         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_287637,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000055 DASQTF010000000
VERSION     DASQTF010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287637"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VE308_01380"
     CDS             complement(<1..462)
                     /locus_tag="VE308_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZA99651.1"
                     /translation="MDARNIGLRNIEVADTKICSIDGENGKLIYRGYDILDLVNHSTY
                     EESAYMLLFGELPTTDQLKDFNRRLIEARGIPEPVIKNLRNRPKRAQPMDILQSCVSE
                     LADYDLNIEDASKEAHIRRAIILIAKIPAIVSAWNRIRSGHHVLDPLDKVSH"
     gene            1116..>2044
                     /gene="purM"
                     /locus_tag="VE308_01385"
     CDS             1116..>2044
                     /gene="purM"
                     /locus_tag="VE308_01385"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZA99652.1"
                     /translation="MTKGITYRDAGVDIDSIHRGQKSIGEIISVTHKMLAIGKVTSGF
                     GHYAGLVKLANETIALHADGVGTKVIVAQMMNKFNTVGIDCVAMNVNDVICVGAQPVA
                     FIDYIALRHPNEWLLQEIAKGLVKGAQQSKMAIVGGETAVLQDIIAGDSENAFDLAGM
                     VMGVVRGKPILGEMIKPGDIILGVESSGLHSNGYTLARKVLLSKYSVDDNTDHLVQTV
                     GEELLIPTRIYVRPVIEILKKRIRLHGLANITGGGFTKLPRLNPKVRYVFDDLPAPTG
                     VFKQIQVDGNIDSKEMYRTFNMGVGFCVVASKT"
BASE COUNT          582 a          416 c          450 g          596 t
ORIGIN      
        1 atgcgatact ttatcgagag gatcaagcac atgatgaccg cttctaatcc ggttccatgc
       61 ggagactatc gcaggtattt tagcaatcag tataattgcg cgtcttatgt gggcctcctt
      121 ggaggcatct tcaatattga ggtcataatc tgctagctct gagacacacg actgaagtat
      181 gtccattggc tgggctcgtt taggcctgtt cctcaagttc tttatgacag gctctggtat
      241 cccacgagct tctatcagcc ttcgattgaa atcctttagc tgatctgtag taggcagctc
      301 accaaaaagg agcatgtaag cactctcctc ataagttgaa tggttgacga ggtcaaggat
      361 gtcgtagcct cggtatatga gcttgccatt ctcaccatca attgagcata tcttggtgtc
      421 tgcaacctca atattgcgaa gaccaatatt ccgtgcatcc atttatcgta acagtgttga
      481 ttccaccaaa tttctatata aagtaatgtt attgttgtca aggtatcatc tcaggaatca
      541 tatattgtag taaactatgt cgtgtataga acttgactta aatagtgaac ttgaaaatac
      601 ttttcaagtc tttcagaata ctctgcttat gtgccatgaa gcttctaaga ttgctttgtg
      661 aattgtcttc aataagcttt taccttcaaa ccactttgca tcatcaatta tcctaagaat
      721 agctgtctct tttcttcctt tttagaactt tgtaatattt tttttagcag ccgagagaat
      781 cgatccgtat agtgccatgc cataatctct agagatgaag tctgttaacc tatcgagagc
      841 agctcgactc tgtaaaagga gggattagta catcactact gatacaactg tatatccttc
      901 ttgatcattt atagcttcag tcccgttatt gatttgatgc tatttcgccg cacattacta
      961 cgctgatcaa tctgtatatc ttattactat acaaccatcg tcagacagga acagtcctca
     1021 atcctataga tatcttcgac atatgatcct tccatgtgag acctcaaagg gtattagtta
     1081 cttattatac agctcaagcc aacgagatgt tgcacttgac caagggaatc acataccgtg
     1141 atgccggcgt cgatatcgat agcatacaca gggggcaaaa gtcgataggt gagataatat
     1201 ccgtgactca caagatgctt gcaattggta aagtaacctc tggttttggg cactatgctg
     1261 gtcttgttaa actagccaat gaaaccattg cactccacgc tgacggtgtg gggacaaaag
     1321 tgattgttgc gcagatgatg aacaaattca atacagttgg catcgactgt gtagcaatga
     1381 acgtaaatga tgtaatttgc gttggcgcgc aaccagtcgc atttattgat tacattgcac
     1441 tgcgccatcc aaacgaatgg ctcttgcagg agatagctaa aggtctagtg aagggtgccc
     1501 agcagagcaa gatggctata gtcggcggcg agacagctgt attgcaagat ataatagcag
     1561 gcgatagtga aaacgcgttt gacctagcag gaatggtaat gggagtggtt aggggcaagc
     1621 caattctcgg ggaaatgatc aagccaggcg acattatctt gggagttgaa agcagtggac
     1681 tccactcaaa tggctataca cttgctcgta aagttttgct ttcaaaatat tcggtggacg
     1741 ataataccga tcatcttgtg cagacagtgg gcgaagagct gttgatccca actagaattt
     1801 acgtaaggcc tgtaattgag attttgaaga aaagaataag gttgcacggc cttgcaaaca
     1861 ttaccggggg cggatttaca aaactgcctc gattaaaccc caaagtgaga tatgtctttg
     1921 atgatcttcc agcacccacc ggtgtattca agcagataca agttgatggt aacattgaca
     1981 gcaaggaaat gtaccgtaca tttaacatgg gggtcggttt ttgcgtagtt gcttcaaaaa
     2041 caag
//
LOCUS       DASQTF010000056         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_196391,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000056 DASQTF010000000
VERSION     DASQTF010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196391"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VE308_01390"
     CDS             <1..944
                     /locus_tag="VE308_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99653.1"
                     /translation="YVVSMEPAEQQIHSKDNMTYCQHCESETVIFDMVSSEFVCSSCG
                     CVADDRIFHGDNAAGIVNPSEYGDRTRTGMPESLAVHHKGLSTLIGLDDTDARGKTLE
                     PAQKMKMQRLRTWNNRSQLNDSISRNLEKAMKFLNNFGDKLYLSQAVMESAAYIYRKA
                     ALKKLAKGRSTLGLVGAALYAACRETATPKTVADVAAVCNISSKDVMAHYKLILRELS
                     LQMPVLHGPDYVTLIANRLDLSEKTKREALRIYTRVQQNRISLGKNPRALAGAVIYLA
                     SQNCNEFLRQVEICQVADISTVSLRKRCKEIKATLGI"
     gene            complement(928..1866)
                     /locus_tag="VE308_01395"
     CDS             complement(928..1866)
                     /locus_tag="VE308_01395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZA99654.1"
                     /translation="MQLAESAKNADDYEFIHRIRQAEYTLQAERRMKRIAGGEVNGGL
                     LEVYDLENMVIISDLHGDSKSLFRILSEINYEQFLSKGLNKLVFLGDYIDRGSDSLSI
                     MYSICYLKSTYPDSVILMRGNHEAPAEFPFYPHDFPYAIEDRFGNRWREIYKKILSMF
                     RLLTLATVVRQKLLLVHGGLPTKEAVAENFREWIASAHESQVRNSVLEEILWNDPRQI
                     DENRGQERSTRGLGRYFGPSVTRTWLQATGTKAVIRGHEPCQGFRLDHDNMIMTLFSC
                     TDVYPNSSAAYVMLNSAKLETIKDAKELSVHVKFLA"
BASE COUNT          672 a          514 c          538 g          660 t
ORIGIN      
        1 aatacgtagt ttcgatggaa ccagctgaac aacaaataca ttctaaggat aatatgacct
       61 attgtcagca ttgtgaaagt gaaaccgtaa tatttgatat ggtttcgagt gagtttgtat
      121 gtagctcatg cggctgtgtt gcagacgata ggatttttca tggcgataac gcagctggta
      181 tcgttaatcc ttctgaatac ggcgacagga caaggacggg gatgcccgaa tcacttgcag
      241 tacatcataa ggggctgtca acacttatcg gtctggatga cacggatgca aggggaaaga
      301 cgctagagcc ggctcaaaag atgaagatgc aaaggctacg tacttggaat aataggtctc
      361 agctcaatga ctctatctca aggaatcttg agaaggcaat gaaatttctg aataactttg
      421 gcgacaagtt gtatctcagc caagctgtga tggaaagcgc agcttacata taccgtaaag
      481 ccgctctcaa aaaactggcg aagggtcggt caactcttgg acttgttggc gcagcgcttt
      541 atgcagcctg cagagagact gccactccaa agactgtggc ggatgttgca gcagtctgca
      601 acatttcaag caaggacgtc atggcgcatt ataagctcat cctgagggaa ctatctctgc
      661 agatgcccgt gctgcacggc ccagactatg tcacactaat agccaacagg cttgacctga
      721 gcgaaaagac taaacgtgaa gccctccgca tctacacaag agtacagcag aaccgtattt
      781 cattgggcaa gaatccaagg gcacttgcag gtgcagtaat ttaccttgca tctcagaatt
      841 gtaatgagtt ccttcgtcag gtagagatat gtcaagtggc agatatatcg acagtctcac
      901 tccggaaaag atgcaaggag atcaaggcta cgctaggaat ttgacatgaa cagagagctc
      961 ttttgcatcc ttgattgtct caagtttggc tgaatttagc attacataag ccgctgagga
     1021 attagggtaa acgtctgtac aagaaaatag cgtcatgatc atattgtcat gatcgagtct
     1081 gaacccttgg cagggctcgt gtcccctgat aactgcttta gtacctgtcg cttgcaacca
     1141 ggtacgagta acactgggcc cgaaatatct cccgaggcca cgtgttgacc tttcttgacc
     1201 tctgttttcg tcaatctgcc gtgggtcgtt ccataatatc tcttcaagca cactgtttct
     1261 gacctggctt tcatgagcag aggctatcca ctctcgaaag ttttcagcca ctgcctcttt
     1321 ggtggggagc cccccatgca ctaagagtaa cttctgcctg acgactgttg caagcgtcag
     1381 cagtctaaac atcgatagta tctttttata gatctctctc caacgattgc caaaacgatc
     1441 ttctatcgcg taaggaaaat catgcggata aaaagggaac tctgcagggg cctcgtggtt
     1501 tcccctcatc agtataaccg aatcgggata tgtgcttttc agatagcata ttgaatacat
     1561 tatactgagg gaatcacttc ccctatcaat ataatctcca agaaatacaa gtttgttcag
     1621 tccctttgat aggaattgct catagtttat ttcagaaaga atccgaaaaa gcgactttga
     1681 atcgccgtgc aggtcgctta ttatcaccat gttctctaaa tcatagactt ccaacaggcc
     1741 gccatttacc tcgccgccag ctatcctttt cattcgcctt tctgcctgga gcgtgtattc
     1801 tgcctgccta atcctatgta taaattcgta atcgtcagca ttttttgcgg actctgccag
     1861 ctgcaatgag atatacggtt ctaggatgac agttaaatgt attacatgtt gggatatcct
     1921 cttaaagatc tgtaattgca agaagatgat tgggcaatcc gttgttttta aatagatact
     1981 aagctacttt ttagctctgt catatatgca actagactta aatagagtag tttctttcgc
     2041 agaaaatggc gcagacttgg ccacttttga attagtaaga agactgcaat tgctattact
     2101 ttatttcagc ccagacctag gaatcttcta gcttcttgtt gggttagaaa gtatgtttaa
     2161 cattaaatcc aataggattt gagtttctgt agtaagttgc cattaaatgc tgagggctgg
     2221 agtaacagag ggtggctaga gataatagaa ttcatactgc tcatgtgtga gaatggggct
     2281 agaaagaccc atgtaatgta tcgatgcaat ctgaactcca agcaaataaa ccagtatctt
     2341 caattcctgc tcgacagcgg aatgcttgag acgatacagg agcg
//
LOCUS       DASQTF010000057         4600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_379240,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000057 DASQTF010000000
VERSION     DASQTF010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379240"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VE308_01400"
     CDS             complement(<1..303)
                     /locus_tag="VE308_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZA99655.1"
                     /translation="MPIDPDFPKNHQPIGKHQHADGEHFHFVWGPGRAAEATENEEVK
                     SAYQTRGEEIVPLGVHGTMVAVDWDSCVADGACIEACPVQVFQWYRTENDVPAVEVA"
     gene            complement(371..1552)
                     /locus_tag="VE308_01405"
     CDS             complement(371..1552)
                     /locus_tag="VE308_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HZA99656.1"
                     /translation="MRLIIYTGKGGTGKTVTSCATAIKMAERNYETLVISADPAHTLS
                     DAFMMHNVGHDPKQVLPNLTALQIDPVTEMSRQYSTILSYTAALFSSKGIDETLAYEI
                     AMLPGMTQLFSLLKVEEVDRTKTFDAVVLDMPASGEALRYLYFPKLVGSIGRKLTGLA
                     GMFSGFAKVFQPFAKLPTPPKDVIQSEMDLLDRLNALSDIINNNDRTSVRLVANPDTF
                     SIENAKRVLMSTSLYGINVDMAVINKIMASSSDEYYAKWASFQRSKVEEAKANFYPLP
                     VREAQLYDTELRGIDMLRRHGEMLFGNDDPSKIFYRGEPYMFVKEGTAINMTVQVPFT
                     EKDDFSIERYGDQLTITVKTVTGQIANVVPLPVATAGMKLAKAKLLNHKLNVQFEKES
                     L"
     gene            1737..2573
                     /locus_tag="VE308_01410"
     CDS             1737..2573
                     /locus_tag="VE308_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZA99657.1"
                     /translation="MPYIKVDEGVSIYVEDIGRERGAKDVLKPVLFIHGWPLSHEMFE
                     YQFMALNKSGYRCIGIDLRGFGLSDKPWTSYNYDVFVDDIKQVISALNLQGVTLVGFS
                     MGGAIVMHYIAKFFSELVSKVVLMSAAAPCFTKREDYPYGLEKSACDDLIIQSKQDRP
                     KMVSDFAKMFFKSENSQSREMLEWLFSINMEASPFATVKCLEELRNADLRNDMQVVNQ
                     RNLPVAIFHGTHDKICPFDFAKVMNKGIKGSRLIEFNESGQGLNIEEKDKTNEELMKF
                     IG"
     gene            complement(2829..4055)
                     /locus_tag="VE308_01415"
     CDS             complement(2829..4055)
                     /locus_tag="VE308_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZA99658.1"
                     /translation="MAIDTGDTTWMLISTGLVMMMTPALGFFEAGLIRGKNSLSVIMQ
                     TFSGLAILSTLWFVLGFTLVFSPSQGGFIGGFDWLFYNNVPFNESLDYSPTIPGVTFA
                     TYQMMFAVITPLLITGAFAERVKWNAFVVFIVAWSIFIYYPLAHWVWGRGWLAELGVF
                     DFAGGIVIHTSAGLASLAAALVLGRRKNFGPDIMVPHNIPLAVIGATLLWLGWYGFNA
                     GSALASGSLPANTLLVTHIAASTSALVWIALSWRRAGRPSSTAAINGAISGLAGVTPA
                     AGFISAQNSFLLGIVLGFASYYAILLLKEHLKIDDALDVSSVHGVTGIVGSLAIGLIA
                     STLINPAGPDGLLYGNPMQLAIQAFGVAVAAALGFGGTILIMKIIDTTIGLKVREEEE
                     DIGLDITQHAERAYVS"
     gene            complement(4129..>4600)
                     /locus_tag="VE308_01420"
     CDS             complement(4129..>4600)
                     /locus_tag="VE308_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZA99659.1"
                     /translation="DTRMLGLNLRLPELSAAVAKVQMQKLDKMLAIRRRNAELLSKLL
                     FASSKKYKVKVPEETADKKFNWYLYTIALHDERDKIKSKLVQNNVGATIYYNPPVHKM
                     PFYVHTSSSETKLPATEWCADHVLSLPVHPHVAEADVDRIANSLKSIIETQNIS"
BASE COUNT         1318 a         1018 c         1026 g         1238 t
ORIGIN      
        1 tgccacctca actgcaggaa catcattctc tgttctatac cactgaaata cctgcacagg
       61 acaggcttca atacaggcgc cgtcagcaac acaagagtcc cagtcgacag ctaccattgt
      121 cccatgaaca cctagaggaa ctatttcttc tcctcttgtt tgatatgcag atttgacctc
      181 ttcgttttct gtcgcttctg cagcccttcc aggcccccat acaaaatgaa aatgctcccc
      241 gtctgcgtgc tggtgcttgc caattggctg atgatttttt ggaaagtctg gatctattgg
      301 cattacttct caaatctgat aaaattcaag attatataaa ctgtttgcag acggcctgat
      361 agagcctagg tcataaagat tctttctcaa actgcacgtt tagcttatga ttcaaaagtt
      421 tcgccttggc aagcttcatt cctgcagttg caactggcaa aggaacaaca ttggctatct
      481 gtcctgtcac agtcttaact gttatagtga gctggtcccc ataacgctct atgctaaagt
      541 cgtctttctc agtaaatggt acctgcactg tcatattgat tgcagttccc tcctttacaa
      601 acatgtatgg ttcgccgcga tagaatatct tggatgggtc gtcattacca aagagcattt
      661 caccgtgtct gcgcagcata tctatgccac gaagctcagt atcatagagc tgtgcctctc
      721 ttacaggcaa aggatagaaa tttgcctttg cttcttccac ttttgacctc tggaagcttg
      781 cccatttagc atagtattca tctgatgagc tggccattat tttgtttatt actgccatat
      841 cgacattaat gccatagagg cttgtcgaca tgagtacacg cttggcattt tcgatactga
      901 atgtgtcggg gtttgcaacc agtctaacag aggtcctatc gttgttatta ataatgtcgg
      961 atagtgcatt gagcctgtcg aggagatcca tttcactctg tatgacatcc ttggggggtg
     1021 tcggtaactt ggcaaatggt tggaagacct tggcaaaccc actaaacatg cctgcaagcc
     1081 ctgtcagctt gcgaccgatg ctgcctacaa gcttagggaa atacagatat cgtagtgctt
     1141 cgccggaggc tggcatatca agcactaccg catcaaatgt ttttgtacgg tcgacttcct
     1201 ctaccttcaa tagcgaaaat aattgagtca taccgggcag catagcaatc tcataggcaa
     1261 gagtctcatc tatccctttg gaagaaaata gggctgcagt ataggagagt atagtactgt
     1321 attgtcggct catttcagtc acaggatcta tttgcaatgc agtcaagttc ggcagcactt
     1381 gctttggatc gtggccgaca ttgtgcatca tgaaagcatc gctcagtgta tgcgccggat
     1441 ctgctgaaat tacaagcgtc tcatagttac gctcggccat cttgatcgca gttgcgcagg
     1501 aagttactgt cttgccagta ccacccttgc cagtgtagat tattagtctc aacaaaataa
     1561 aaaagtgaat acaagccaat aaaagacttt agatatctaa ttcagggatg ctggtgccta
     1621 gatcccatct catatcaaaa tggtggttga acactctaaa aaagcaacta accatttgaa
     1681 gcaatcgatt ttcaaggaaa cattataaat agaagaataa gttgaagcaa taataaatgc
     1741 cttatattaa agtcgacgaa ggcgtatcta tttacgtgga agacataggc agagaaagag
     1801 gggcaaaaga tgttctcaaa cctgtcttgt ttattcacgg atggccattg agtcacgaga
     1861 tgtttgaata ccaattcatg gcactgaata agagcggcta ccgctgtatc ggtattgatc
     1921 ttagaggctt tgggctctca gataagcctt ggaccagcta caactatgat gtatttgtgg
     1981 atgatataaa acaagtgatt tccgcactca acttacaggg agtaactcta gtaggatttt
     2041 caatgggagg tgcaatagtc atgcattata tagcaaagtt cttttcagaa ctggtttcaa
     2101 aagtagtatt gatgagtgca gctgcacctt gttttaccaa gagagaggat tatccttatg
     2161 gacttgaaaa gtcagcttgc gacgatctca tcatacaatc aaaacaagac aggccaaaga
     2221 tggtctctga ctttgccaaa atgttcttta agagtgagaa ctctcaaagc agggagatgt
     2281 tagagtggct attctcaata aatatggaag catctccatt tgcaaccgta aagtgcctag
     2341 aagaattacg aaatgcagat cttagaaatg atatgcaggt agtgaatcaa agaaacctgc
     2401 cagtagcaat atttcacggc acacatgaca agatatgtcc atttgacttt gcaaaagtga
     2461 tgaataaagg aatcaaaggt tctagactga ttgaattcaa tgaaagcggg cagggtctga
     2521 acattgaaga gaaagacaaa acaaatgaag aactaatgaa gtttattgga tagcagagtg
     2581 ttagcagaga taccgaaata atgtgatata catatctgta aattagccaa aagctcggca
     2641 catgtacatt tcttgtaaaa ctatcttgtc aatagagtgt gtaacttagg ttatagcatg
     2701 tcaaagaaaa accacacatt tcgaattttg caatagcatc ttatagcgat atataacact
     2761 atcctaatgt tctttacata atcctaaaag agagtataaa tcagaaaaga aaagaaaaaa
     2821 ttaaaacatt agctgacata cgcccgctct gcatgttgcg taatgtcgag cccaatgtct
     2881 tcttcctcct ctcttacctt gagccctatt gtggtgtcta ttatcttcat gatgaggatt
     2941 gtgccgccaa agcctaaagc agctgctact gcgacgccaa atgcctgtat tgctagctgc
     3001 attgggtttc catatagcaa cccatctggg ccagcagggt tgataagagt gcttgcgata
     3061 aggcctatcg ctagtgagcc cacgatgcca gttacgccgt ggacagagct gacgtcaagt
     3121 gcgtcatcga tcttcaggtg ctcttttagt agtagtattg catagtatga agcaaatcct
     3181 agcacaatgc caagcagaaa tgagttctgc gcactaatga aacctgcagc aggcgttacg
     3241 cctgcgagcc ctgaaatcgc gccgttgatg gcggctgtac tcgatggcct gcccgcccgc
     3301 ctccacgaaa gcgctatcca aacgagagcg gatgtggatg cggcaatatg cgtcacaagc
     3361 aacgtgtttg caggaagcga tcccgaagca agtgcactgc ctgcgttaaa cccataccat
     3421 ccgagccaga gtagcgtcgc tccgatgact gcaagcggta tattgtgagg caccatgata
     3481 tcggggccga aattcttcct gcgtccaagc actagcgcag ctgcaaggga ggcaagacct
     3541 gcacttgtat gaatgacgat accgccggca aagtcgaata cacccagctc tgcaagccat
     3601 cctcttcccc atacccagtg tgcaagagga tagtagatga agatgctcca tgccacgatg
     3661 aagactacaa acgcattcca cttgaccctt tctgcaaagg cgcctgtgat gagaagtggt
     3721 gtaatgactg caaacatcat ctgataagtg gcaaacgtca cgccgggaat tgtcggcgaa
     3781 tagtcgagag actcattgaa gggaacgttg ttatagaaca gccagtcaaa gccaccaata
     3841 aatccaccct gggatggaga gaaaacaagg gtgaatccaa gaacgaacca gagggtagaa
     3901 agaatggcaa gtccagaaaa ggtctgcatt attacggaca gcgagttttt gcctcggatg
     3961 agaccagctt caaaaaatcc gagcgccggt gtcatcatca tgacaaggcc agtggagatc
     4021 aacatccagg tagtatctcc agtgtctatc gccatttccg ccttctagtt ggcggatcca
     4081 taatataaaa acgcatcggc aacttggata ttaacctaac tgtaaaattt atgatatgtt
     4141 ttgcgtctca attatagatt ttaggctgtt tgctatacga tctacatctg cttctgcgac
     4201 gtgagggtgt acgggcagcg acagcacgtg atcggcgcac cattctgtag ctggcagctt
     4261 tgtttccgac gaggaggtgt gtacatagaa tggcattttg tgcactggcg ggttgtaata
     4321 gatagttgcg cccacattat tttgcacaag tttgctcttt atcttatccc tctcatcatg
     4381 aagggctata gtgtagagat accaattaaa cttcttgtcg gcagtctcct caggaacctt
     4441 tactttgtat ttctttgagg acgcaaacaa cagtttgctt aaaagctccg cgttgcgtct
     4501 cctgatcgct aacatcttgt ccagcttttg catctgcacc ttagcaacag cggcgctcag
     4561 ctcaggcagt cgaaggttca gaccaagcat ccttgtgtca
//
LOCUS       DASQTF010000058         5418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1305760,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000058 DASQTF010000000
VERSION     DASQTF010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305760"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VE308_01425"
     CDS             <1..324
                     /locus_tag="VE308_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZA99660.1"
                     /translation="LLVVAIVTKIGSGYIGAKIVGFSREVSLAIGFLMNGRGMVELVI
                     ASIGFAAGVIDMTLFSIAVAIGLVTTILAPLTSRPMISSAKSKGSDAVLIEDSSGKEW
                     QSEMA"
     gene            790..966
                     /locus_tag="VE308_01430"
     CDS             790..966
                     /locus_tag="VE308_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99661.1"
                     /translation="MVRNQRRRLLRNQRRVGETIIIASGIGVALLGSFLGVQFVSYGG
                     LCIAGLGIISIIWR"
     gene            complement(1516..2805)
                     /locus_tag="VE308_01435"
     CDS             complement(1516..2805)
                     /locus_tag="VE308_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99662.1"
                     /translation="MPPFRLFKKKSSDIPSAEQSVPTPATDTLTIQQAQDLLESLESV
                     KVKELSARLARVKESAVESLKVIDILAKAMDRENVKLEGMEQRLKSVVEHSKKTVVSS
                     LKREAGLELPSPQSANDAKKFKERFENMMKRIGEVSGSHSKVLNAFMKKHSGKMKDEF
                     EFLTKLLNETRAIISEFDRDREPIIRCGNMLNTALQKMSSIKLSESSANNIEKEIEDI
                     EGELNKLESELAATSSSNEFEQASISMRNIAEAEKKQEAFHAKTKDLFSHLSRAFTKY
                     SYGITKDTEQRLRTMSHEPWEIFYEINVEPYLSLLLEIRRSIDTGQIQLKDSDKVLQY
                     LDTIIESLPDLQQKAQALKIEIDSFRELNIDIVYKLKGLEEKIMQNTEVLTRSRQDLE
                     QQRRQAKDKKEEVHAILKEAGEILAELTGQKYSLRYD"
     gene            2885..3664
                     /locus_tag="VE308_01440"
     CDS             2885..3664
                     /locus_tag="VE308_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99663.1"
                     /translation="MATGEGGFSSPNRRSSKKGTGKAYLWFQRNESKLLVIDIVCVVL
                     LFVMHLFPSTFQFFIHEKVNAIQIAAIMLVSFIAVSVVWKGIVPIVICVLGIVLIHNS
                     VILPYYSQPQPGEAYFGEVKFRWTLYTPTAVSMGAQMNFFLGVSMVIFSIIIAYRPSL
                     LFTRNRPESVDSEWSKYPLWHDNILLADGKTEYSVPIKRLVTDQERYVLWRYEYILAS
                     IYGTPHLVRPEGYVPKYSTGIYRDRESGRILGKGRYNGFFV"
     gene            4097..4555
                     /gene="rnhA"
                     /locus_tag="VE308_01445"
     CDS             4097..4555
                     /gene="rnhA"
                     /locus_tag="VE308_01445"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZA99664.1"
                     /translation="MLIEVYFDGLCQPINPGGISCYAFVVKSGGRTIHSDYGVAGEPF
                     SEDSTNNVAEYTALAKALEWLLANNLGSTKVEIKSDSQLIVNQLTGDYKVKAKRIISL
                     YKQVLLLKSKFKEIQINWIPREKNKEADKLTNKAYNKALQENPGYLDRIK"
     gene            complement(4773..5153)
                     /locus_tag="VE308_01450"
     CDS             complement(4773..5153)
                     /locus_tag="VE308_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99665.1"
                     /translation="MCEDVLAKHNSIRWTGIVNKNGVILAQKARKAVKLLLSDEENEE
                     YAATAIARQKTRGKFEPKIGKMVYSFGRYELIHRATIPINENYYLLMTFDVEEKNFDQ
                     IIIDKILPVVTKSRNHFVATDDLV"
BASE COUNT         1511 a         1117 c         1136 g         1654 t
ORIGIN      
        1 ctacttgtag ttgcgatagt cactaaaatt ggttcaggat acattggcgc aaagattgta
       61 ggattcagcc gtgaagtaag tctggcaata ggctttctta tgaatgggag gggaatggtt
      121 gagcttgtaa ttgcctctat cggatttgcc gcaggggtta tagatatgac tttgttttcg
      181 atagcagttg caatagggct tgttaccaca atactagcac cgctaacatc gcgtcccatg
      241 atcagcagtg ccaagtcaaa aggcagcgat gcagtcttga tcgaagactc atcaggtaag
      301 gagtggcaat cagaaatggc ttaactgcca tttctccact tttgctcaga atatctgatt
      361 ctaaaaccta tcaaaaaatg agcgtcactg ctggtcttgt tcttgttttg cttaggtaca
      421 gagaaaaatc tatgaggata gtttggcatc tgatgctgca attactatgc ttttgcttac
      481 ctaaagctac caaacagact ttcaatatgc caaaaaatat acaagatttg ttactccttt
      541 tctacattca accctgtctt ggatagtgcc tgtggtatac ccaattgtcg ctatatgctt
      601 tttggcttca tgataacccc cagtggagat tatgcgcact catgagcaaa gaaaaaccct
      661 ggtgaaaaac ccacgagagc aaaaacaaca aacaggaaaa caaaagtgtc tcaagcgatg
      721 atagcatctt ggtcagaaga ttacagggcg caggtaattt tataaaacca acgcaggata
      781 tcatttttca tggtgcgcaa ccagaggcgc cggcttttga gaaatcagag aagagttggc
      841 gagaccataa ttattgcaag tggcattggc gtagcgctgc ttggctcatt tcttggagtc
      901 caatttgtgt cttatggtgg actgtgcata gcaggtctcg gaatcatatc cataatttgg
      961 agatagtgca taagatgcaa aactatatcg gttgccttat taggctgtcc agaattgttg
     1021 cagtgtttaa agtaaaaatt tagcaataga atatgaccgt taacaacgag cgagcggcgc
     1081 gacagcatac ggagccgcat ctgaattctc cgctccgacg atagcgacac acttgtgcac
     1141 gcgagcataa aacgattgac tagggtggga tttcatttgt acctttgtgt gcattataac
     1201 agcttgagtc agaaaattac gtcttcaaag atagacttat tcgtcggaaa acagaagtag
     1261 cacttgagag acatcatgga gataacggag gcccaatcag cagcttaata aaaaaagcaa
     1321 cacgatgaca cgcacctata tgcagttagc tccaatacag caaaaataat tgggttctca
     1381 gtaataaaaa tgacctcgtg tcgacatgct tagagtcccc taagccgtat cgtttcagct
     1441 gtagacaaca tgtcaggctc tttagcggag gcaatctttt gcttatacag gattgtgaat
     1501 aggattaaac aaaacttagt catatctcag ggagtatttc tgacctgtaa gctcagccaa
     1561 gatctcacct gcttccttca atatggcatg tacttcttct ttcttgtctt ttgcctgccg
     1621 cctctgttgt tctaggtctt gcctgcttct tgttagtact tctgtgttct gcataatctt
     1681 ttcttcaaga cctttaagtt tgtaaactat atctatattg agctctcgaa atgaatcaat
     1741 ttctatcttg agtgcctgag ccttttgctg aagatcaggc agagattcta ttatagtatc
     1801 taggtactga agtaccttgt cggaatcttt aagctggatt tggccagtat ctattgacct
     1861 acgaatttcg agcaggagag ataagtatgg ttcaacattt atttcataaa atatctccca
     1921 cggctcatgg gacatagtcc tgagacgctg ctctgtatct tttgtaatgc cgtaagagta
     1981 tttcgtaaat gctcttgata agtgggaaaa gagatctttg gtttttgcat ggaacgcttc
     2041 ttgcttcttt tctgcttcag ctatgttacg catgctgatg gaggcctgct caaattcgtt
     2101 ggaactactg gttgcagcaa gctcactctc aagcttgttt agctctcctt ctatatcttc
     2161 aatttccttc tcgatgttat ttgctgaaga ctcagataat tttatagatg acatcttttg
     2221 cagagcagta ttcaacatat ttccacatct tattatgggc tcacggtctc gatcgaattc
     2281 tgaaattatc gctcttgtct catttagcag cttggtcaga aattcaaatt catctttcat
     2341 cttgcctgag tgtttcttca taaaggcgtt gagcactttg ctgtgcgagc cactcacctc
     2401 tccgatgcgc ttcatcatgt tttcaaacct ttccttgaat ttcttggcgt catttgcgga
     2461 ttgaggtgag ggaagctcca agcctgcttc gcgcttgagc gaggatacaa ctgtcttctt
     2521 tgagtgttca acaacggact ttagtctctg ctccatgccc tcaagcttga cgttttctcg
     2581 atccatagct ttcgccagta tatcgatgac cttgagagat tctactgcag actctttaac
     2641 tcgggcgagg cgagcagaaa gttctttcac ctttacagat tcaaggctct ctagcaaatc
     2701 ctgtgcctgc tgtatcgtaa gtgtatcagt agccggcgtt ggtaccgact gctcagccga
     2761 tggaatatcg ctagattttt ttttaaagag cctaaatgga ggcactgtaa ttctttctag
     2821 ctgacagcat caaaaacgtt acgatctagt aatgcttaat gcaaatggtt gccacattag
     2881 tagcatggca acaggagagg ggggtttctc cagcccaaac cgtcggagca gcaagaaagg
     2941 gactggtaag gcctatcttt ggtttcaaag aaatgagtcg aagctgctcg tcatagatat
     3001 tgtctgcgtt gttctcctct tcgttatgca tctttttcct tcaacatttc agttcttcat
     3061 acacgaaaaa gtaaatgcta tccaaatagc agcaataatg cttgtttctt ttatagctgt
     3121 gtcagttgtg tggaaaggaa tagttccaat agttatatgc gtcttaggaa tcgtgcttat
     3181 acataattct gtgatactgc catattactc acagccccag cctggcgagg cttactttgg
     3241 agaagtgaaa ttcaggtgga cgctgtatac accaacagct gtcagcatgg gtgcacagat
     3301 gaactttttt cttggggttt caatggttat cttcagcata ataattgcct accggccgtc
     3361 tttactcttt acgagaaata ggcctgaatc ggttgattct gaatggtcga agtaccctct
     3421 ttggcatgac aatatcctac tagcagacgg caagacagaa tactcagttc cgataaagag
     3481 acttgtgaca gatcaagaaa gatacgttct ttggcgctat gaatacattt tggcaagtat
     3541 ttacggaact ccgcatcttg ttaggcctga aggctacgtc ccaaaatact cgaccggcat
     3601 ttacagagat cgagaaagcg ggcgcatctt aggcaaaggt cgctataacg gcttttttgt
     3661 ttaattggtc tcctaatcta gaatatattc aacttgcagc caaacgtggt aagctggccc
     3721 tgcttttctc ggcgtatttt acatctaata atagattcat tcgtattacg ctacacttta
     3781 aagtagctta tcgacggcta caggcaaagt tatgtattgt ttttatatgg tgatctttag
     3841 acctcggatt aatttttcaa aagcccatta ttgcttacag tttgggtcta ttatttagct
     3901 tagaatcagc tatatagtct caaaacacct gtcttgtatg acagacctaa gtctgagagc
     3961 ccccaagaca aaaagtcttt tttgaggccg aatgaatcca tcgcttcaga agcacattag
     4021 ctattgcaag aggttatgcg gctctaccct attaatgagg ggacacagac atatctgtgg
     4081 cagtcaatat tcagttttgt tgatagaagt ttactttgat ggcctctgtc agccaataaa
     4141 ccctggtggc atttcatgct atgcttttgt agtcaaaagc ggtggaagga ccattcatag
     4201 tgattatggc gttgctggag agcctttctc agaggactct accaacaatg ttgcagaata
     4261 cactgccctt gccaaagcat tagagtggct cctcgcaaac aatcttggct ccaccaaggt
     4321 tgagatcaag agcgattctc agctcattgt caaccagcta acaggcgatt acaaggtgaa
     4381 ggcaaaaaga ataatctcgc tttacaagca ggttcttctc cttaagagca agtttaagga
     4441 gattcagatc aactggatac caagggaaaa gaataaagaa gctgacaagc taacaaacaa
     4501 ggcgtataac aaggcactac aagaaaaccc aggatatctt gacagaataa aatgagaaga
     4561 acgaagacag gtctaggcaa ataaattact agtcagatag tttgttgagc ggtgcgattt
     4621 aagatctatg cctcaaacta aaaagtatcg agtaatataa caatgataat cgcgtgttgc
     4681 atccttaata ctattataag ttctcatgct actttttgtt tcatcctcta gcatgtacgt
     4741 aagacaaagt cgaatgcgtg atcacactga ggtcagacca aatcatctgt ggcgacaaag
     4801 tgattcctgc ttttggttac gactggcaag atcttgtcta ttatgatctg gtcgaaattc
     4861 ttttcttcaa cgtcaaaagt cattaacaga taataatttt cgtttattgg tatcgtagca
     4921 cggtgtatca attcatatcg gccaaatgag tacaccatct tccctatctt gggttcaaac
     4981 ttaccccttg tcttttgtct agcgattgct gtggctgcat attcttcatt ctcttcgtct
     5041 gagagcagaa gctttaccgc ttttctagcc ttctgtgcca atatgacacc gttcttattt
     5101 actattcctg tccacctaat cgagttgtgc tttgcaagaa cgtcctcaca cactgaattt
     5161 agaagctcat aatcgatttt caatttgcct acatcggggt atagttcgta gcctaattaa
     5221 atagctggtt atagctatat attatcacct taattccata tttgcagacc aatgtctgaa
     5281 tatgagtctg gaaccttccg atgcagggat tgtgcaaagg aatttctgac aaaagatgag
     5341 gctgataggc attatataga gcaccactca gatgtaatag tcggcgaata aattttttaa
     5401 cataagcttg cgaatcta
//
LOCUS       DASQTF010000059         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_471775,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000059 DASQTF010000000
VERSION     DASQTF010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471775"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..455)
                     /locus_tag="VE308_01455"
     CDS             complement(150..455)
                     /locus_tag="VE308_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99666.1"
                     /translation="MNLRNGPDNSSNNNNNRTAKATAAIPIAGILVAAALLLGLLSVI
                     GSETAIAQQNMTGTNATAMNATAMNATAMNATAMNATAMNATAMNATAMNATGITGG"
     gene            complement(1267..1995)
                     /locus_tag="VE308_01460"
     CDS             complement(1267..1995)
                     /locus_tag="VE308_01460"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HZA99667.1"
                     /translation="MTSSADQQQPPPPINDWDSTVGRNVKTVDYQHAGKVIGAAENED
                     TIIISYEGPHSSYNYRVPKRYVQGFNDPDLLLSIARAELADYEISDIQDYAAHLRSIK
                     TGKKGGGEEEITVPVIEEKLNISKKVITEEATIIKEPFMQTKILEVSVMHEEIVIEKR
                     AAASEEGDTTAAEYPPPKNTRTVIRVPLIHEVVRIKKEPYVKEEILIRKKPRTETKTV
                     SEFVTTEKVDTSNNNNNNTIGQTS"
     gene            complement(2031..2765)
                     /locus_tag="VE308_01465"
     CDS             complement(2031..2765)
                     /locus_tag="VE308_01465"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HZA99668.1"
                     /translation="MSQNPVESKNSEISPGDAVVNWDRIIHKNVRTLDNQGMGKVIAV
                     PNDEDTIIISSQSGGEQYKIPRSCVSGFNGAEVLLNETAYKISSSYRVDNAGAYEAKP
                     SDIITEERQQEGKEEETTVPLIEERLKVSKKTSTTQYTITKEPVTEKKTIEVPLTHEE
                     LVVEKRPAKDSSPAQGPVKSKTEIKVLLNREEVEVTKKPFVKEEVVVKKKPVTETRTV
                     SDTVTSERINMSGNAAVRQGTVATQE"
BASE COUNT          677 a          578 c          556 g          982 t
ORIGIN      
        1 ttgagtgtat cgttctgggt tccaccggac tcgccttcgt gacaaaaact ttaaagctaa
       61 tagtctcatt tttagtctta tagagcaaaa aattaggagt atgagagact gaggagctct
      121 ctctctgttg ttattgttga gttttcatat taaccgcctg ttattcctgt tgcgttcata
      181 gctgttgcgt tcatagctgt tgcgttcata gctgttgcgt tcatagctgt tgcgttcata
      241 gctgttgcat tcatagctgt tgcattcata gctgttgcat tagtgcctgt catgttctgc
      301 tgtgctatag ctgtctcact gccgatgacg gataataatc ctaacaggag cgctgcagca
      361 actaatattc ctgctatagg tatagcagct gttgctttgg ctgttctatt gttgttgtta
      421 ttgctgctgt tatctggtcc attacgtaga ttcattaatg aaaatgagag agtatgaaac
      481 ttaactagtt ctatacacga taacaaacaa taagctagat gaatatgaga taagtttgat
      541 gtgaagagaa gtaaagaaca tcttcagctt tgcagacagc ttgcaaaagg aaatacctgc
      601 gattgatgat atccggatga tctagtatag actaacattt tattgcttat ttatcagggg
      661 ttacccaagg aagacctcag tgtctgcttg ttgttctgtc atgtccctat cgacaactcc
      721 ggtacagacc ttttctcaaa atgtaattta acaacaagaa tggaatacat tgttgaagag
      781 acaatgttga gttttttcag acgtgatgag ttaagaacaa agctgcaatt gccttaatgt
      841 ttcaatatgg gttgccatca tttgtagtat tgtcaaagag cgagccccca gtctgcttgt
      901 tgatgccctg cgtgatataa tattgcgcat ctacaaagaa ggattagagg aaaaggtttc
      961 ttctatgtcg atactttttc aaatgcccga gagcattgac aaccttagac cgagctatta
     1021 ttgacatgct aaagttgata ctccattatc tatacaaacg gccttgagtt ttacaaaata
     1081 aggacgcaag atgggaatcc cgatataata ttgcttactg ctttgaaatg tattaaccaa
     1141 gagttaaaaa atatttttca gatatgacgg tacagtcctg cataatagag gcaaagcttt
     1201 gcaagggctc tagcggtaaa gaaagaggga tcagtcatca tctaatatgg cgagtccttt
     1261 tgaaattcaa gaagtttgac caatggtatt attattgtta ttattactcg tatctacttt
     1321 ctctgtagtc acaaactcac tgacagtttt tgtttcagtt cttggctttt ttcttatcag
     1381 tatctcttcc ttaacatagg gctctttctt tatcctaact acctcatgga ttaacggaac
     1441 acgtatcaca gttcttgtat tctttggagg agggtattct gctgccgttg tatcgccttc
     1501 ttctgatgct gctgctctct tttctatgac tatctcttcg tgcataacgg aaacttctag
     1561 gatctttgtt tgcatgaaag gttcttttat tattgtggct tcctcagtta tgaccttctt
     1621 tgatatattg agcttttctt caatgacggg tacagttatc tcttcttctc caccgccttt
     1681 ttttcctgtt ttgatgctcc ttaagtgtgc ggcatagtct tggatatcgc tgatttcata
     1741 atctgccagt tcagctctgg ctattgaaag cagcaaatcc ggatcgttga agccctgcac
     1801 gtatcgctta ggcactctat aattataact gctgtgagga ccctcatatg aaattattat
     1861 tgtgtcctcg ttttcagctg cgccaattac tttacctgca tgctgatagt ccacagtctt
     1921 gacatttctg ccaacggtgc tatcccagtc gttgatgggc ggtggtggtt gctgttgatc
     1981 agcggatgat gtcatttttt attctctctg ccttgagact gccatcgcga ctattcttgc
     2041 gtcgccacag tgccctgccg cacggcagca ttgcccgaca tgttgattct ttcacttgtc
     2101 acagtatcac ttacagtcct agtttcagtg acaggctttt tcttcaccac tacctcttcc
     2161 ttaacaaatg gctttttggt tacttcaacc tcttctctgt tcaatagtac tttaatttct
     2221 gtcttcgact ttacaggacc ttgtgcaggt gaggagtcct tagcaggtct tttctctaca
     2281 acaagttctt cgtgggtaag tggtacttct attgtctttt tctcagtaac aggctccttt
     2341 gtaatagtat attgagtagt tgaagttttc tttgacacct tcaaccgctc ttctatgagt
     2401 ggaacggtgg tttcttcttc ttttccttct tgctgacgct cttcggtgat aatatcgctg
     2461 ggttttgctt catatgcacc tgcattgtca actcgatagg aagaagagat cttgtaagca
     2521 gtttcgttaa gtaatacttc tgctccatta aagccggata cacaagacct cggtatcttg
     2581 tactgttcgc ctccactctg tgatgagatg attattgtat cttcatcatt aggcaccgca
     2641 atcactttac ccatcccctg attgtccagc gtcctgacat ttttatgaat tatcctgtcc
     2701 caattaacaa ctgcatctcc aggagatatc tctgagtttt tggattcaac tggattctga
     2761 ctcatagcag gcatttcatc cttgtgttgt tag
//
LOCUS       DASQTF010000060        11264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1213607,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000060 DASQTF010000000
VERSION     DASQTF010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213607"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VE308_01470"
     CDS             <1..326
                     /locus_tag="VE308_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99669.1"
                     /translation="GTHMTATSDIFNVKTLKDSIKFYLPRVEGYLEIVRGMASKYGGM
                     SLIEFDGYFEGKFEPVKYTKVEIHTNDIDEQCMMKAANDIRIALKQESFAFEFNNKLI
                     LVSEP"
     gene            complement(506..1324)
                     /locus_tag="VE308_01475"
     CDS             complement(506..1324)
                     /locus_tag="VE308_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZA99670.1"
                     /translation="MSDHDPFSDWSELINRPVYAVDGKKVGFLRSIIADYLLVNKGFI
                     VLNKYYIPKTLAESVDRKGRIRLRITTFEVRANYTYSKMKDVLTATGYIPEWKVKQSS
                     SYDRLQTLRYSATRNRLAACIAFVSAILFLASGYKANIEIYNLVASEVLSIQSLRQFW
                     NLVIVPIGFLAILSQLGGITVMMGAGFFAANRVNFGKILVMIGTGQGLFTIAIRMLVE
                     LWSGQLWSLNNYVIWLTSTATGLGILFAVVAPTIAKGKGDSIISKTIRLILRRR"
     gene            2468..4144
                     /locus_tag="VE308_01480"
     CDS             2468..4144
                     /locus_tag="VE308_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZA99671.1"
                     /translation="MSHTVDSKTANVNVNRGEQQCVDLSSASQFFDIKTIKDEKSAFS
                     RGKGLTSYLRYCCSSLWFIFSFLHSSVILEFFVHIVGSNPIALPKRVGIVSFGVPILF
                     IILLVNLPTVAAGQETLIQEEPEVDPFRIPADDQPAAINDPNLKMDEIATDLNHPTAI
                     AFLGSSDDIIVTDKDNGTVRRIIGGVIQPEPLIDVAVANNNDTNERGLLGMGVAKQNE
                     TTTYVFLYFTESGGGQDGDDSNGIVPAGNRLYRYELVENANANSTSAELINPKLLLDL
                     PARPGPRYNGGPLLVTQNQNDTIVYLMIGDLDHRLSQAENYEDGPPPDGTGGILAVDA
                     EGNALASPLLVAQVDGDEENGDEENGDEEIGMLPYYFAYGLRNGFGLAFDPVTGYLWD
                     TENGPDWFDEINLVLPGFNSGWAAVQGPASAPENEEADIEEDLVDFGGTGVYRDPEFA
                     WQNTVGVTAINFLNSTALGEQYANDMFVGDINNGRLYHFDLNPDRTGLLLEGVLEDRV
                     ADSAQELEPVLFGTGLRSISDIEVGPDGYLYVVLYGPGKIIRIAPAAAEG"
     gene            4554..4832
                     /locus_tag="VE308_01485"
     CDS             4554..4832
                     /locus_tag="VE308_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZA99672.1"
                     /translation="MPGAYVMINTLPGMEKPVLHQIAQIPGVVSVEGVFAEFDLIVRV
                     DVPLGSTVDLVINEIRKIQGIKSTRTISSIDDERSSGIGMDHLGPPSD"
     gene            complement(5094..5975)
                     /locus_tag="VE308_01490"
     CDS             complement(5094..5975)
                     /locus_tag="VE308_01490"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HZA99673.1"
                     /translation="MAIVGAAGKMGYWFTSYFAQRGLGVSAYDINQKKILKTSGKVKI
                     QNNLIQCVQDADLVLVCVPVQRTPQVIKECTKNMKDGAIIAEISSVKNKTFAALKRIP
                     VNLRPLCIHPMFGPGASKKVKTRLLLVPVRNEEIELKIANEIFENANVMVLPDVKQHD
                     KAIAIVLGLTYFANIVFAKVMSSGNISMLKQVSGTTFGLQSLIAESIFTEEPDLIIAL
                     IGENAYAKRYINQYLKEASALARLAIAKDSNDLKAELQRVKLNMQKRQDIQQSYHRMY
                     EILDKQSKHKNSHSDTL"
     gene            complement(5986..7374)
                     /locus_tag="VE308_01495"
     CDS             complement(5986..7374)
                     /locus_tag="VE308_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZA99674.1"
                     /translation="MDNTNEELDTLRDEVQGVTTQIMRAVQQRIELARKIGEVKSRLG
                     MDIKDEKVEHEIRMRVMLLAEEIGMSTEFALQLLNILLAESQQVQVQKSFQKAVEKQT
                     HLGIFSKAKQLEASGKKIIHMEVGEPDYPPPARVGTALSESFRMKLYHYTDTQGIEKL
                     RDAIAAKEGGGICKDQVLVTPGGRFAVFSAITSLLRAGQELIVIEPAWPAYKECADFV
                     GARTKILRTALENEWIPDTKQLEGMINSSTKMIALNYPNNPTGKVLDHKTMEKIVLIA
                     RDHGLYLLSDEVYADYVFGEFSSIRKYDYARSIIVGSFSKRYAMTGFRVGYGIANKDI
                     ISKMARVQAVAITSVAEPMQQAALAALEVDPSENIKLMKRRLDFVCSKLKKMSLRYVE
                     PDGAMYAYPELPKGEDIPLVEKLLEKGVAIAPGSGFGDSYKRFVRISACQPEKMLAKG
                     LDVMASVIREQV"
     gene            complement(7385..8467)
                     /gene="aroC"
                     /locus_tag="VE308_01500"
     CDS             complement(7385..8467)
                     /gene="aroC"
                     /locus_tag="VE308_01500"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZA99675.1"
                     /translation="MIGERFVAMSFGESHGRCVGIIVDGCPAGLPLEEADIQLLLDLR
                     KPGQSVITTQRKEEDKVEILSGLFNGFTTGAPICMLIWNSDSDAKPYEMIKNIPRPGH
                     ADYPAMLRYGGFADYRGSGRFSGRLTATLVMAGAIAAKLLKITLGIETVAYTTEIGSI
                     RARNITKENIATRYDNEVRCPDFQAAQSMKEAILQARKSGDSLGGIIECISSGLPVGM
                     GEPIFASLESDLSRALFSIPAVKAVEFGSGFAGSKMHGSENNDSYLIKDGKVITKTNN
                     AGGILGGLSNGMPIVARVGFKPAASVAKRQQTLDISTNEQTTLTVPGRHDPCVVPRAP
                     PVVECVVSMVLADHAIRSGLIPLVLK"
     gene            complement(8525..9826)
                     /gene="aroA"
                     /locus_tag="VE308_01505"
     CDS             complement(8525..9826)
                     /gene="aroA"
                     /locus_tag="VE308_01505"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZA99676.1"
                     /translation="MTSIVVRRSRLDGSMRCPSSKSYSHRAIAIASLADSHSTITHVL
                     MARDTLATLTGCCALGADIKYDATKMHIKGHNSFTPPENVINAENSGTTIRILAAMAG
                     LVRTGYTVLTGDESLRNRPMQPLLDALRQLGVAAYSTKENGTAPLIVKGGGIKGGTTV
                     VDGSISSQFISGLLIASIYADSEIVMKIRGNIVSKPYVHATLATMKHFGVSIEHSPDM
                     LEYYIKSARYHGTRFDVPSDFSTAALILAAGALAGEKLRVNGLNFEMPQGDSAIIDIL
                     KKMGCKIKVDEEKGEATISGADRLEGGDFNLSDTPDLLPVVSILALKATSPVTITGVA
                     HARVKETDRVSNIAAELLKFGAQIKEFHDGLKITAPLPIKNALLEAHNDHRLFMAFTI
                     ASMMTEKSIVAGAQSVDVSYPNFISDMKNIGGRISPAPNRE"
     gene            complement(9819..10685)
                     /locus_tag="VE308_01510"
     CDS             complement(9819..10685)
                     /locus_tag="VE308_01510"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR01920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZA99677.1"
                     /translation="MTTIRRARAIVHGAISIVNAIATGKGSALGISLKVTVEAELQKG
                     QGLRLITGRNADRLIRNIIQNTMPKEIIENNMISIRVDSEIPIGYGLKSSSAVSNAVA
                     LACSQMARDEDINDYTILEVAARSSLEAKVSLTGAYDDATACYFGGFTVTDNYSRRLI
                     HKERAPENLYAIIILPSNTQRGDVRKLRNLSEFFIDAFGFAESGQYWKAMKLNGVLAS
                     AALSSSYSPVMTAMEHGALSASISGNGPSIAAVGYEDSIEAIVAALSKFEGRIIVSKV
                     NNEKANVNIIND"
     gene            complement(10682..>11264)
                     /locus_tag="VE308_01515"
     CDS             complement(10682..>11264)
                     /locus_tag="VE308_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZA99678.1"
                     /translation="NTVNNIEGIFRGYNTDVYGFIEPLRKRQVDFRSMHVLLLGSGGA
                     ARAVVVALAEAENGVAKLIIANRDVQRANELAKLASDLGMKCELVPLDRAQDSSPTCD
                     LIVNATTLGMNNESSVIDYQHIQKGSIVYDIVYRPMVTDLIENAKYAQASVVYGYEML
                     IEQGAKAFEIWTGLSAPRDAMKKNLLGLFGEPM"
BASE COUNT         2943 a         2571 c         2380 g         3370 t
ORIGIN      
        1 atggcacgca tatgacggca acctcagata tattcaatgt caagacccta aaggactcaa
       61 taaaatttta tttgcctcga gttgaaggtt atttagaaat agtacgcggt atggcatcca
      121 aatatggtgg tatgtctcta atagagttcg acggttattt tgaaggcaaa ttcgagccgg
      181 tcaaatatac caaggttgaa atccacacca atgatattga tgagcaatgc atgatgaaag
      241 ctgctaacga tatccgcatc gcactcaagc aggaaagctt cgcctttgaa ttcaacaaca
      301 aattaattct tgtgagtgag ccgtagcttg taaccaatta ttccttgcat aatcacttct
      361 ataaccagca accattaact gaagtgagac gagcaataca acaatatgtc ctatcaagtt
      421 gagatgacaa ctaataaggg agatgccacg agatctgaag agaaaagatc cgtttcaaat
      481 gtaaagcgac ctcagagatt atattttacc tccttcttaa gatgagtcga attgtcttag
      541 aaattatgct atctcccttt cctttagcta tagttggagc aacgactgca aacaatatac
      601 caagaccagt agcagtagat gtcaaccata taacataatt attcaggctc cacaactggc
      661 ctgaccaaag ttctactagc atacgaatag ctattgtaaa taggccctgt cctgtgccga
      721 tcatgactaa tatcttgccg aaattgactc tattagcagc aaaaaatcct gctcccatca
      781 ttactgttat tcctcccagt tgactcaaaa tcgccaggaa acctattggg actataacca
      841 agttccaaaa ttgtcggagg ctttgaatgg acaaaacttc agaggccact aggttataga
      901 tttctatgtt tgccttatag ccagaagcta aaaacaagat ggccgaaaca aaggcaatac
      961 acgcagctaa cctatttctt gttgcactat atcttaatgt ttgcaagcga tcatatgagg
     1021 aactttgttt aaccttccac tcagggatgt atcctgttgc tgttaacaca tctttcattt
     1081 tggaatacgt atagtttgca cgtacctcaa atgtagttat cctcagtcga atccttcctt
     1141 tcctgtctac tgattcggca agagtttttg gaatataata tttgttcaga acgataaacc
     1201 ccttgttaac tagtaagtag tctgcaatta ttgagcgtaa aaagcctacc ttcttgccgt
     1261 caacggcata taccggtcta tttataagct cagaccagtc agagaagggg tcatggtctg
     1321 acatttgaat aataatgaaa gcccatccgc tgtattaaca ttagacttca catattattt
     1381 caatgaagac tatccaggta taatcgcctg ttgaggggtg atttatgtgt tagaggatcc
     1441 tttctgcacg tacattgtta gtgccatctc tggccttctc tgataccatg gccaaggtca
     1501 tcgtataatt actgaatatc gtcctgactc atgattacga gattgaaatg caagtaacgc
     1561 tcacaactat gctactggtg actatactat ttatgatggc tattaatcct ctttcggttc
     1621 gcgctgaagg acagctatct cactttagag ataaaagcat gttagatagt gttattttct
     1681 tgctgttaga tgaatttgga tatgcttttg acatttacgg tgcacaaatt ttccccaatg
     1741 acaattaaag tatagcatac tcacagaaga taatcagtca gttttcaata tttctcattt
     1801 gggctttgcg ataatggatc atactattaa tgggtcaaat gtagaaatac atgtcagacc
     1861 cgcaatactt gacgatacaa ggacccgact tgatatccaa ggctatacca ctgccgcgga
     1921 cgctaccagt caatggatga gcaaaagcta taacaatctc cagataagat ctatatggca
     1981 tatatgatag aactactgac aaaaatggtc gttcatgttc ctttagtact gttttgcccc
     2041 cctgctacaa tagttgatta atgaatctgt acctcccaat tacggctcaa aaaagcgctt
     2101 agcctcaagg atgttcgaat gatcgtagat attatcatgc acgaagcatg aagcattata
     2161 gccacgttga tttccggctc ctattgatat gcaaaagcaa cgtgctagat acgaacattc
     2221 atctaagtca tcgacaacac aaataggctt tgtcctcatc aatattcgta atgcgaatat
     2281 gcatgaatga tgacttacat cttaaaaatt tgaagaagga ttcattctaa ccaacttaga
     2341 tagataaaga ttttgaagta aaaataagat aaaacttagc agccaagcaa tttatacaat
     2401 ggctcaaaga acagttaaat atatagccga ctagatgttg agacttgaag ggaagctaat
     2461 aatctcgatg tcacatacag tcgactccaa aacagctaat gtgaatgtta accgcggtga
     2521 acaacaatgt gtcgatcttt catcagcatc tcagtttttc gatattaaga ccataaaaga
     2581 tgaaaagagt gcttttagtc ggggtaaagg ccttacatcg tatctacgtt actgctgctc
     2641 ctccctttgg ttcattttta gtttcctgca ttcaagtgtc atccttgaat tctttgttca
     2701 tattgtggga tcaaatccta ttgcactgcc taagagggtg gggatcgtat catttggagt
     2761 accgatttta tttattatct tgttggtcaa tttacccaca gttgctgcag gacaggagac
     2821 tctgatccaa gaagaacctg aagtagaccc ttttagaata cccgctgacg atcagcctgc
     2881 tgcaataaat gatccaaatc ttaaaatgga cgagattgct accgatttga atcacccaac
     2941 agcaatagcc tttctaggtt ctagcgatga catcatagtg actgacaagg acaatggtac
     3001 tgtacgaagg ataattggag gtgtgataca accagagccg ctaatcgatg ttgctgttgc
     3061 aaacaataat gatacaaacg aaaggggctt gctcggaatg ggtgtagcca agcagaacga
     3121 aactactacg tatgtctttc tttattttac cgagtcagga ggtggacagg acggggatga
     3181 ttccaatgga atagtcccag ccggcaaccg tctttacaga tacgagttgg tagagaatgc
     3241 aaatgctaac tccacctcgg ctgaacttat aaatccaaag ctcttgttag accttcccgc
     3301 aagaccaggt cctcgttaca atggtggccc gctgttagtt actcagaatc agaatgatac
     3361 aatcgtttat cttatgatag gagatttgga tcaccggctg agtcaagctg agaattatga
     3421 ggatggcccg ccaccggacg gaacaggggg catactggca gttgacgctg aaggcaatgc
     3481 attagcaagt cctttacttg ttgcgcaagt cgatggtgat gaagagaatg gggatgaaga
     3541 gaatggggat gaagagatcg gaatgttgcc ctactatttt gcctatggct tgcgaaacgg
     3601 ctttggcttg gctttcgacc ccgtcacagg gtatctatgg gatacagaaa atgggccaga
     3661 ttggttcgat gaaatcaacc ttgtgttgcc gggatttaat agtggttggg cagcggtaca
     3721 aggaccagct tcagcaccag agaatgagga ggcggacata gaagaagacc tagtggactt
     3781 tggcggaacg ggagtatata gagaccccga atttgcatgg caaaacacag taggggtgac
     3841 agcaatcaat ttcctaaatt ccacagcgct aggggagcag tatgcaaatg acatgtttgt
     3901 aggggatata aacaacggca ggctatatca tttcgatcta aacccagaca gaacaggtct
     3961 acttcttgag ggagtgcttg aggaccgagt ggccgatagc gctcaagagc tagagcccgt
     4021 attatttggt acgggattaa gaagcatttc agatattgaa gttggacctg atggttatct
     4081 gtacgtagtg ttatatgggc cgggaaaaat aattagaata gctcctgctg cagcagaggg
     4141 ttgaaagtga tatcggaata gcgagctata agtgcttaaa tattagcaaa aaattcctgt
     4201 ttcgattatt atagcctcct tatgcctaat tgccctttgt tttgtttttg tgtttttgtt
     4261 tttctcctca cttttgatgt tgtatcatcg ccaagcgaga ttttatctag cttgttttaa
     4321 gcatcttgtg aattcttact tagcggccac caacttcgtt ttagcttatc agctcttagt
     4381 gtctcaagat cggctcaaat ctctgatgga agacaaatgt ttgaatctta aatgcgatcg
     4441 tgattacacg aaatcgaaaa gtgttgcatt ttaattccaa gtgagctgta ctgttcggca
     4501 gatcgcatgc cacgcaataa tcgtttaata taagccaggg gttaccaact gctatgccag
     4561 gagcctatgt catgatcaat acacttccag ggatggaaaa gccagtcctt caccagatag
     4621 ctcagattcc tggcgtcgta tcagttgagg gagtctttgc cgaatttgat ctgatagtaa
     4681 gagtggatgt tccattgggc agtacggtcg atcttgtaat caatgagatc agaaaaatac
     4741 aaggcataaa gtcaacaagg actatatcat ccattgatga cgagcgcagt agcggaattg
     4801 ggatggacca ccttgggcca ccatcggact agaaactgtt tattactggt agccatgcag
     4861 tggtttatct ctgtgtgtgt ttatttttga ttctagctgt tttttgtgtt gagaaagtat
     4921 gtgcttgttg gcacattgat catgcatgat tcctatatcg caaaagtcac aggaggtgac
     4981 aaattctagt ttggtaaaac gcttctcgca atagctacag ttttctgctc caagatcaga
     5041 atctgacaat ggcttgagcc acgggtcgcg ccagagaagc gccatatata tgcctacaac
     5101 gtatctgagt ggctattttt gtgtttactt tgtttgtcaa gaatctcgta catgcggtga
     5161 tatgactgct gtatatcctg tctcttttgc atatttaact taactctttg caactcggcc
     5221 tttagatcat tgctgtcctt tgcaattgcg agccttgcaa gtgctgatgc ttctttaaga
     5281 tattggttaa tgtatctctt ggcgtatgca ttctcgccaa taagagcaat tataaggtca
     5341 ggttcctccg tgaatatgct ttctgcaata agcgactgga gaccgaatgt cgttcctgac
     5401 acttgcttta gcattgaaat gttgcctgat gacatgacct ttgcaaaaac tatatttgcg
     5461 aagtacgtta atccaagcac tatcgctatt gccttgtcat gctgcttgac atctggcaat
     5521 accatgacat tggcgttttc aaatatttca tttgctatct tcagttctat ttcttcattc
     5581 cttaccggaa ctagaagcag ccgagtcttt acttttttac ttgccccggg accgaacatt
     5641 ggatgaatgc agaggggtcg aagattaact gggatcctct tcaaagcagc aaatgtctta
     5701 tttttaactg aagatatttc tgcaatgatg gccccatcct tcatgttttt tgtgcattct
     5761 tttatgactt gtggcgtcct ttgcacaggc acacagacaa gtaccaagtc tgcatcctga
     5821 acacactgga ttagattatt ttgaattttt accttgccgg aggttttcaa tatttttttt
     5881 tggttaatat catatgctga aacgcccaga ccgcgctggg caaaatagct ggtaaaccaa
     5941 tagcccattt ttcctgcggc gcctacgatc gccacctgct ccaatttaca cctgctccct
     6001 tatgaccgat gccattacgt ctagcccttt tgctagcatc ttctccggct gacaagcaga
     6061 tatccttaca aatcgcttgt atgaatcccc aaatccacta ccaggtgcta tcgctactcc
     6121 tttctctagc aacttttcaa ctaggggtat gtcctctccc ttcggaagct ctggataagc
     6181 atacatcgct ccgtccggtt cgacatacct aagtgacatt ttcttaagct tgctgcatac
     6241 aaagtcaagt ctccttttca ttagtttgat gttttctgat gggtctacct caagagcagc
     6301 aagagcagcc tgctgcattg gctctgctac gcttgtaatt gcaactgcct gaactcttgc
     6361 catttttgag ataatgtctt tgtttgcaat accatacccc accctaaagc cagtcattgc
     6421 atagcgtttt gaaaaagatc ctacgataat gcttctagcg taatcgtatt tgcgtatgct
     6481 gctgaactcg ccaaagacat agtcggcata cacctcatcg ctgagcaagt aaagaccatg
     6541 atccctggcg attaacacta tcttttccat tgttttgtga tcaagcacct ttcccgtggg
     6601 gttgtttggg tagttgagtg cgatcatttt agtacttgaa ttaatcatcc cctcgagttg
     6661 tttagtgtcg gggatccact cgttttcgag ggcagttctg agtatctttg tcctcgcacc
     6721 tacaaaatct gcacattctt tataggcagg ccacgctggc tcgatgacta ttagctcttg
     6781 tcccgctctt agcaatgacg taattgcgct gaagacggca aagcggccgc caggcgtaac
     6841 caagacttga tctttgcata ttcctccccc ttctttcgcg gctatagcat cccgtagctt
     6901 ctcaatgcct tgagtatctg tatagtggta taatttcatc ctgaaagatt cagaaagcgc
     6961 ggtcccaact cttgctggcg gcgggtagtc gggttcccca acttccatgt gtattatttt
     7021 cttgcctgaa gcctcaagct gctttgcctt tgaaaatatg ccaaggtgcg tctgcttttc
     7081 tactgccttt tggaagctct tttgcacttg cacctgctgt gactctgcaa gcaagatatt
     7141 gaggagctgt agagcaaact cagtgctcat tccgatctct tctgccagca acatgactct
     7201 catccgtatc tcatgttcaa ccttttcatc tttgatatcc atgccaagtc tgctctttac
     7261 ctcccctatc ttccttgcaa gttctatacg ctgctggaca gcgcgcatta tctgcgttgt
     7321 gacgccttgg acctcgtcac gcagtgtatc gagctcttcg tttgtattat ccatcagcac
     7381 tgtctcactt taacaccaat ggtattagtc ctgaccgtat ggcatgatcg gccaaaacca
     7441 tcgaaactac acactcgacg acaggtggag cccttggtac aacacatggg tcatgtctac
     7501 ctggcacagt aagagttgtc tgctcgttcg tagaaatgtc aagcgtctgc tgcctcttgg
     7561 ctactgaggc ggctggttta aagcccaccc tagcgacaat cggcattcca tttgatagcc
     7621 cacctaatat tccacctgca ttattggtct tggttatcac cttgccatct tttatcaaat
     7681 agctatcgtt attttcagag ccatgcattt tgctgcctgc aaagccagaa ccgaactcta
     7741 cagctttgac tgcaggtatg ctaaataagg ccctgctgag atctgactca agtgatgcaa
     7801 atatgggctc gcccatgccg accggcaggc cgctgctaat acattcaatt attccgccca
     7861 gcgagtcgcc tgattttctt gcctgcagga tcgcctcttt catgctctga gcggcttgaa
     7921 agtcggggca tctcacttcg ttatcatatc tagtagctat gttttctttg gttatattgc
     7981 gtgctctgat gctacctatt tcggtagtat aagccaccgt ctcaatgcct agcgttattt
     8041 ttaaaagctt ggcggctata gcgccggcca tcacaagagt ggccgtcagc cttccggaga
     8101 accttccact ccccctatag tctgcaaaac caccgtatct tagcatagca ggatagtctg
     8161 catgtcccgg ccgtggaatg ttttttatca tctcataagg ctttgcatct gaatcactgt
     8221 tccatataag catgcatatt ggggcgcctg tggtaaaacc attgaagaga ccagacagaa
     8281 tctcgacttt gtcttcttct tttcgctgtg tggttataac cgactggccg ggctttctta
     8341 ggtcaagcag tagctgtatg tcggcttcct ctagaggcag gccagcagga caaccatcta
     8401 caattatccc gacacacctt ccatggctct caccaaagct catggcgaca aaccgctccc
     8461 ctatcatgct gccacccatc tctattagag agcacttgag cctgttctat atattggctt
     8521 tttgttattc tctattaggg gctggggata ttcttcctcc gatgtttttc atatcggaaa
     8581 taaaattcgg atacgaaacg tctacagatt gagctccagc tactatcgat ttttcagtca
     8641 tcatagaagc aattgtaaag gccataaaaa gtcggtggtc gttgtgcgct tcaagcaatg
     8701 cattttttat tggtagaggg gcagttatct ttagcccatc atgaaactct ttgatctgcg
     8761 cgccaaactt taggagctcg gctgcgatgt ttgacaccct atctgtttcc tttacccttg
     8821 catgagccac gcctgttatt gtgaccggag acgttgcctt gagtgcaagt attgaaacaa
     8881 ccggcaggag atcaggtgtg tcggatagat taaagtcccc gccttctaat ctgtcggcac
     8941 cactgatagt tgcctcaccc ttttcttcgt ccacttttat cttgcatccc attttcttca
     9001 agatgtctat aatcgcggaa tcaccctgtg gcatttcaaa gttgagccca tttaccctga
     9061 gtttttctcc ggcaagcgca ccggcggcaa gtataagcgc ggcagttgaa aagtcgcttg
     9121 gtacatcaaa tcttgtgcca tggtatcttg cgctcttgat gtagtattca agcatatctg
     9181 gactatgctc aatagaaact ccaaagtgct tcattgtagc aagcgtggca tggacatagg
     9241 gcttggagac tatgttgccc cttatcttca ttacgatctc cgaatcagcg taaatgctag
     9301 caatcagtaa acctgatatg aattgacttg aaatgctgcc gtcaactaca gttgtccctc
     9361 cctttattcc tccacccttt actataaggg gcgccgttcc gttttccttt gtggaataag
     9421 ctgccacgcc tagctgtcgc agagcatcta gtaaaggctg catagggcga ttccggagac
     9481 tttcatctcc agtcagtacc gtgtagccgg ttctcacgag gccagccatg gcagcaagta
     9541 tccttattgt ggttccagaa ttctctgcat ttattacatt ttcaggtgga gtaaagctat
     9601 tgtggccctt tatgtgcatc tttgtagcgt catattttat gtctgctccg agagcgcagc
     9661 agccggttaa ggtcgctagg gtatcgcggg ccattagtac atgtgttatt gtagaatggc
     9721 tatcagcaag cgaggcaatt gctattgccc tgtgactgta gctcttacta gatgggcagc
     9781 gcatgcttcc atctaaccta gatctcctta ctactatact agtcatttat gatgttcaca
     9841 tttgccttct cgttatttac tttggaaaca attattctcc cctcaaattt tgacagggca
     9901 gcaactatgg cttcaattga gtcttcgtat cctacagcag caatggaggg accatttccc
     9961 gatatgcttg cagatagtgc cccatgctcc atggcagtca ttacaggtga ataacttgat
    10021 gaaagtgcag cagaggcaag gactccgtta agtttcattg ccttccagta ttgacctgac
    10081 tctgcaaaac cgaaagcatc gatgaagaac tcagacaggt tcctcagctt gcgcacgtcc
    10141 cctctttgag tgttgctcgg taaaatgatg attgcataaa ggttctccgg cgccctctcc
    10201 ttgtggatta gccgtctaga gtagttgtca gtgacagtaa agcccccaaa gtagcaggcg
    10261 gtagcatcat cgtatgctcc tgtcagtgag accttggctt caagcgagga acgggcagcc
    10321 acttcaagta ttgtataatc gttaatgtcc tcgtctcttg ccatctggct acatgcaagc
    10381 gcaacagcat ttgacactgc actggagctc ttgagcccat acccaattgg aatttctgaa
    10441 tcgactctta tacttatcat gttattttcg ataatttctt tcggcattgt gttttgaata
    10501 atatttctta tcagcctgtc agcattcctc cctgtgatta acctaaggcc ttgtcccttt
    10561 tggagttcag cttcgaccgt aactttcaat gatatgccaa gtgcggatcc ctttccggta
    10621 gctatggcgt tcactatcga aattgcacca tgcactatag ctcttgccct tctgattgtg
    10681 gtcacatcgg ttcaccaaag agtcctagca ggtttttttt catagcatcc ctaggagcag
    10741 acaacccggt ccatatctcg aatgcctttg caccctgctc gataagcatt tcatagccgt
    10801 atacaacgct tgcttgtgca tattttgcat tttcgatcag gtcggtgacc attggacgat
    10861 acacgatatc gtagactata cttcccttct ggatatgctg ataatcgatt acgctagatt
    10921 cgttgttcat tcctaatgtt gtcgcgttaa ctatcagatc gcacgtaggc gaggagtcct
    10981 gtgctctgtc cagcggcact agctcacatt tcatgccaag gtcagacgct agcttggcaa
    11041 gttcgtttgc tcgctgcacg tcacgatttg caattataag cttggctacg ccgttctccg
    11101 cttctgcaag ggcaactacc accgcccgcg cggccccacc agatccaagg agcagcacat
    11161 gcatactcct gaaatcaacc tggcgcttgc gcagtggctc gataaagcca tagacatcgg
    11221 tattgtatcc tctaaatata ccctctatgt tgttaaccgt attt
//
LOCUS       DASQTF010000061         7801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1306476,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000061 DASQTF010000000
VERSION     DASQTF010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306476"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VE308_01520"
     CDS             <1..616
                     /locus_tag="VE308_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZA99679.1"
                     /translation="VDYVKKGMMPAFASVSQLMQMSTASIAVNLTGIRESVYNILGES
                     SSEVLQKVLKTAAEVTIGQGKQLGEEEAGITMVSDDSGTRFAALDSEKYGKISLLQSQ
                     NTTSYSQGMILNGRDILNDGEASIRECLAIDKLLNRGFAASLDLTNLSPDEMKSAIDA
                     TVQELPFLRLNIGFAVCTTCGQRSKGGVDICEFCKSPHKVPLYL"
     gene            896..3625
                     /locus_tag="VE308_01525"
     CDS             896..3625
                     /locus_tag="VE308_01525"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HZA99680.1"
                     /translation="MAGVTEVNNNSKETLVAKIRKRDGRIVHFEQSKISNAIYKALIA
                     TGKPDYPLAERLSAKVVQKMVQQSEKIVPSVEDAQDMVESILIEEGLSETAKAYILYR
                     HERRKVRDEKMKVLNKKDLDEVDKAFDINSLRVLAARYLLRDNNNEILEGPKQMFERV
                     ATLVAIADVMHDSSIFAHAGGYTQNIEEADRYYGKLEDFDNKLHIGSYYLNRYHFEAM
                     IRHYIYCAQQGQMKISFKDLLRTIAEGKMAQYEDRIREYYNLMVSRDYLPNTPTLMNS
                     GAKLGQLSACFVLDMPDDMAGIMKASTDAAMIFKSGGGVGINYSDLRPEGDIVASTSG
                     VASGPTSFMRIIDTITDVVKQGGKRRGANMGILEAWHPDVEKFVTAKTKPGVFENFNV
                     SVGIWDDFWKALVSKDGNHKYPLHSPRMKESMRHIDAQQLVDLIALSAWKSAEPGVIF
                     FDNINKYNPLIGARGGPLRATNPCGEQSLYPYESCNLGSINLANFVKRKADGAYEFDW
                     QRYEQAIRLATRFLDNIIDMNKYPVEEVKVATKETRRIGLGIMGIADLLFLLKISYDS
                     KDGYEFMNKLAEAVSYLSMDESVAIAKSRGPFPLFKNSDYVKGRIPVAGYYELPKETH
                     TYDWDTLIEKIKKHGIRNSWTSTIAPTGTLSMIADTSNGVEPLFALVYEKRVTVGRFF
                     YADKVFENMLKENGLYSDEILTKIANNYGSVRGLPEIPEWMQRIFVTAIDIHWTDHVM
                     AQAVWQKWISNAIAKTINMPGDVTAEDVKCAYLLSHELGLKGVTVFRDGSRHEQVLHI
                     TGQNTGERAFAVTPSKYVIDYVLDSIKEPYVLEQMQKIFKESGIGDEKPAATMALTPQ
                     EVQSGVKNSASIAAIMQHTDIQENQCPTCNARLIITEGCNVCIECGFSGCGSG"
     gene            4029..4607
                     /locus_tag="VE308_01530"
     CDS             4029..4607
                     /locus_tag="VE308_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZA99681.1"
                     /translation="MSSVKINESPNHFMVLDAIGRGVKSFDKIAKVTRLDKAEVELII
                     NDLIGQRLVIRTEKKGFLRTKVELTLSHTGLQMLNSKKQELEKQMQQIQQWQSNGQTQ
                     QLQSYMDNNRMWMPMMLFSGIMNAVFFMSMVSMLGMAMTPAESGLAGGEGSGTAADSG
                     ESAAGAEDSGAAADSGGGADFGGDFGGGDFSF"
     gene            complement(4597..5442)
                     /locus_tag="VE308_01535"
     CDS             complement(4597..5442)
                     /locus_tag="VE308_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZA99682.1"
                     /translation="MILEVLEFGFMQRALIAGVSVAITSSVIGLFLVLRRNSLFGDAL
                     SHAAFGGIAVGLATNIYPLWTGLALSILGALGITKLRQYAKIPADATVAILLSSGLAL
                     GILLISLSGGFTLDLFSFLFGSIMLVSVEDTLAILAMTGIILSIIILLYRQLMYITFD
                     EEQARVSGLPISKLNYLFIILASVAVIVSMRLVGILLVSSLLVIPNVTALLFNKGFKK
                     TAIISVSISIFSVVTGIAISYAANLAPAGTIVLVSTAVFLTVLAARHYSKKKQTVKKL
                     EEARS"
     gene            complement(5453..6199)
                     /locus_tag="VE308_01540"
     CDS             complement(5453..6199)
                     /locus_tag="VE308_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HZA99683.1"
                     /translation="MQIVEIDRLSYAYSGNLVLDNISFTIEEGDILGIIGPNGAGKTT
                     LFSCMLGLLDDYTGTIKILGENIEKKNNKVFKSIGYIPQKKTIEQNFPATVEEIVSLG
                     VTTTGKTSKEKIALALETAGLLAQKDRRVGELSGGQQQRVLIAKAMVNNPKLLILDEP
                     VTGIDLEMQNKFYSLLKRLNQENNITIIWASHDLDAVNRFATSVACINRSMFFHGKTI
                     EFFENPDLLKAYSESSMQAHMHLHNHTYRG"
     gene            complement(6190..7146)
                     /locus_tag="VE308_01545"
     CDS             complement(6190..7146)
                     /locus_tag="VE308_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZA99684.1"
                     /translation="MNQSRAALAAIATLIPLAMIATVQTGRPALQDRQEQEKITVVAS
                     FYPLYEFASRVVGDRAEVSSLVPAGVEPHDWEPTAEDVSRGRAADVLVINGAGFERWA
                     DDIEAKVVANTSEGIEFNYKEGREVGEDEHRHGDASGGGGVNNPHIWLDPILAKHQID
                     KIRNMMASSDPVNADYYNQNADRFTAELDSLDAFIRSELADCDKSHFIAFHDAFTYFS
                     ERYGLRQHSIQGASPEGEILPQTMQQIIELANEFDINIIYSEDLIDSRLADTIANEIP
                     EGKVLVLSPIEGIDKGEQAAGIGYIDKMKQNIANLKEGLECR"
     gene            7239..7622
                     /locus_tag="VE308_01550"
     CDS             7239..7622
                     /locus_tag="VE308_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HZA99685.1"
                     /translation="MAIVSISLNNDILTEIDKLQKALGFSGRSEIVRAGIRNLLAEEK
                     DRQDLYGELFAVLLAIHDEKSDDQVTEMQHDYDKLITTHIHNKIDRDRCLEIFLLKGE
                     AAEIKNMTKKFQSNKKMDHVKLIAM"
BASE COUNT         2218 a         1715 c         1817 g         2051 t
ORIGIN      
        1 cgtcgactat gtcaaaaagg ggatgatgcc tgcatttgcg agtgtaagcc agttgatgca
       61 gatgagtaca gctagcatag cagtcaacct tactggcatt agggaatccg tctacaacat
      121 actaggagag tcctctagcg aggtgcttca gaaagtgcta aagactgctg ccgaagtgac
      181 catcgggcag ggcaagcaac ttggagagga agaagctggc attacgatgg tttcagatga
      241 cagcggcaca aggtttgccg cgcttgattc tgagaagtat ggtaagatct ctcttctgca
      301 atcgcagaat acaaccagct attctcaagg gatgatactc aatggcaggg atatccttaa
      361 tgacggcgaa gcttcaattc gagaatgtct ggcaattgac aagctgttga atagaggttt
      421 tgcagcatcg cttgatttga caaacctgtc gccggacgaa atgaaaagtg caatagacgc
      481 tacagtacaa gagctaccat ttctgcgcct caatattggg tttgcagtct gcacaacgtg
      541 tggtcagcgc tcaaaaggag gtgtagatat atgcgagttc tgcaagtcgc ctcataaggt
      601 gcctctgtat ttgtaactgc aataaatgct aaaaccgctc gccgaaccct tacatcattc
      661 ttttttgata tgatctaagc tagcagctta tgctgtagtt tgtatagttt gctaagaaaa
      721 tcgatgtgcg tgcgtgcatg cgctctatct ttttctatat ctagaactta gttttatttt
      781 atcttaccat gcaagataca tctaataaaa acttagatcc atgttttagt attaatatca
      841 gagcaagttt ccccagtgga tatataggtt acaagtaacc ttcagtacat ctgggatggc
      901 aggagtaact gaagttaata ataattcaaa ggaaacgctg gtcgcaaaga tacggaaacg
      961 tgacggacgc atagtccatt ttgagcaatc caagatttca aacgcaatat acaaggcact
     1021 cattgcaaca ggcaagcctg actaccctct ggctgagcgg ctaagtgcaa aggtagtaca
     1081 aaagatggtt cagcagagcg aaaagatcgt tccaagcgta gaagacgcac aggacatggt
     1141 ggagtccata cttattgaag aggggctgtc cgagacagcc aaggcctaca tactctacag
     1201 gcatgaaagg cgtaaagtgc gcgatgagaa gatgaaggtt ctcaacaaga aggatctcga
     1261 tgaagttgac aaggcctttg acattaattc cttaagagtg cttgctgcgc ggtatttact
     1321 gcgcgacaac aacaatgaga tccttgaagg gcctaagcaa atgtttgaaa gggttgcaac
     1381 cctcgtcgcg attgccgacg taatgcatga ctcttctata tttgcccatg ctggcggtta
     1441 cacacagaac attgaagagg cagatcgcta ctacggtaaa ctagaggact ttgacaacaa
     1501 gctacacatt ggtagctact atctgaacag gtaccatttt gaagcaatga tccgtcacta
     1561 catctactgc gctcagcagg gacagatgaa gatcagcttc aaggatctgc ttaggacgat
     1621 agcggagggc aagatggcgc agtatgaaga ccggattagg gaatactaca acctaatggt
     1681 ttcacgggac tatctgccaa acacgcccac actgatgaac tccggcgcaa agcttggaca
     1741 actgtcggcg tgttttgtgc ttgacatgcc cgatgacatg gcaggcatca tgaaagcatc
     1801 cactgacgca gccatgatct tcaagtcagg cggcggagtg ggcatcaact actccgacct
     1861 aaggccggag ggcgacattg tcgcatcgac atcaggcgta gcatccgggc caacatcttt
     1921 catgcgcatc attgacacca ttaccgatgt cgtcaagcag ggaggcaagc gccgcggagc
     1981 caacatgggt atccttgagg cttggcatcc agacgttgaa aagtttgtga cggctaagac
     2041 aaagcctgga gtatttgaga actttaatgt aagcgtcggc atctgggatg acttttggaa
     2101 ggcgcttgtc agcaaggatg gcaatcataa gtatcctctg cacagtccaa ggatgaagga
     2161 gtccatgagg cacatcgacg cgcagcagct tgtagacctg atcgcattga gcgcgtggaa
     2221 aagcgctgaa ccaggagtga tattctttga taatattaac aagtataatc cccttattgg
     2281 agcaagaggc gggccactgc gtgccacaaa tccatgtggt gagcaatcgc tctaccccta
     2341 tgaatcatgc aatctcggct cgatcaactt ggcaaacttt gtcaaacgca aagctgatgg
     2401 cgcatacgag tttgactggc agcgctacga gcaggcgatc agacttgcga caagatttct
     2461 agacaacatc attgacatga acaagtatcc tgtagaagaa gtcaaggttg cgacaaagga
     2521 gacaaggaga atagggcttg gcatcatggg cattgctgat ctgttgttcc tgctcaagat
     2581 ctcatatgat tccaaggatg gctatgaatt catgaataag cttgcagaag ctgtgtccta
     2641 tttgagcatg gatgaaagtg tagccattgc aaaatctcgt ggaccattcc cactatttaa
     2701 gaacagtgat tatgtcaagg gccgaatacc tgtggcagga tactatgagc tgccaaaaga
     2761 gacgcataca tacgactggg acacacttat tgaaaagatc aaaaaacatg gcatacgtaa
     2821 ctcgtggaca tcaaccattg ccccaactgg cacgctctca atgatcgcag atacttcaaa
     2881 tggtgttgaa ccgctgtttg cacttgtgta tgaaaagcgc gtgactgtgg gtcgcttctt
     2941 ctacgctgat aaggtgtttg aaaacatgct gaaagaaaac ggcttgtact ctgatgaaat
     3001 actgaccaag attgcaaaca actatggttc tgtccgcggc ttacccgaga ttcctgagtg
     3061 gatgcagcgg atatttgtga ctgcaatcga catccactgg acggatcatg tgatggcaca
     3121 ggccgtgtgg cagaaatgga tcagtaacgc cattgcgaaa actatcaata tgcctggaga
     3181 cgtgacagcc gaagacgtca aatgcgcata tctgctatct catgagcttg gattgaaagg
     3241 cgttactgtc tttagagatg gttctcgtca cgagcaggtg cttcacatca ccggccagaa
     3301 caccggcgaa agagcatttg ctgttactcc cagcaagtac gtaatcgatt atgtcctcga
     3361 cagtattaag gaaccctatg ttctagagca gatgcaaaag atattcaaag aatcagggat
     3421 aggagacgaa aagcctgcag caactatggc tcttacgcct caagaagtgc agtctggcgt
     3481 aaagaattct gcttcaatag ctgctataat gcagcatacg gacatacaag agaaccagtg
     3541 cccgacgtgc aatgcaagac tgatcattac cgaaggatgt aatgtgtgca tcgaatgcgg
     3601 ctttagtggc tgtggctccg gctaggagtc tatctactac tactaacctt gataggagat
     3661 gaggtacctg gcaaagatag tgaaggacgt caactagaca atcgatctca acgaaagtgt
     3721 ctcggggaca ttgacaccag ttcgttgcag aaattgcaag cgcgaactcg caccacatgc
     3781 gaattttgag tattgcaatg actgcagcgt gtactatgtg ggcaagtggc gtatacagca
     3841 gtagtcctta acccccatct tgttttttgc cacttttctt atttgataat taaaacgttt
     3901 tctgttatag ctgttgctgg ctatcaatcc gagatgaaca ccttgttaca gcagaagaat
     3961 gagcttgtga tgtgacagaa cctctaagaa tagagtttaa attctgcttt gtgcatatta
     4021 ctagtgaaat gtcaagcgtc aaaatcaatg agagtcctaa ccactttatg gtgttggacg
     4081 ccattggaag aggagtaaag tcttttgaca aaattgccaa ggttactcgt ttggataagg
     4141 ccgaagttga gctaatcata aatgatctga ttggacagag gcttgttatt cgcactgaaa
     4201 agaaaggttt ccttagaact aaggtagagc tgaccctcag ccacactggg cttcagatgc
     4261 tcaactccaa aaagcaggaa ttagagaagc aaatgcagca gatccaacaa tggcaaagca
     4321 atggccagac gcagcagctt caaagctata tggacaataa ccgtatgtgg atgccgatga
     4381 tgttgttctc cggtatcatg aacgctgtgt tctttatgtc catggtatcg atgttgggga
     4441 tggcaatgac tcctgcagag agtggtcttg caggcggcga aggatctgga acagctgcgg
     4501 attcaggtga atcagctgca ggagccgaag acagcggtgc cgcagcagat agcggtggag
     4561 gcgctgattt cggcggcgac tttggtggtg gagattttag cttctagcct cttctaattt
     4621 tttgactgtt tgcttctttt ttgaataatg cctagctgca agaacagtca gaaatactgc
     4681 agttgagaca agtactattg ttcctgcagg cgccagattt gcagcatatg aaattgcgat
     4741 acctgtcact actgaaaata ttgatattga cacagatatg atagccgttt tcttgaatcc
     4801 tttgttaaac aaaagcgccg taacatttgg aatcaccaaa agcgacgaga caagtaggat
     4861 tccgacaagc ctcattgata caatgactgc cacacttgca agaattatga aaaggtagtt
     4921 tagctttgaa attggcaggc cgctaacccg cgcttgctct tcatcaaatg tgatgtacat
     4981 cagttgtcga tataggagga tgattataga cagaattatt ccagtcattg ccagtatcgc
     5041 aagagtgtcc tctacgctga caagcatgat gcttccaaac aaaaaactga acaggtcaag
     5101 cgtgaaccct cctgataggc ttataagtaa tatgccaagc gcaaggcctg acgaaagcaa
     5161 tatggcgaca gtcgcatccg ccggtatctt ggcatactga cgtaactttg tgatgcccaa
     5221 cgctcctagt atagaaagcg ccagaccagt ccaaagagga taaatgttag tagcaagccc
     5281 tacagctatg cctccaaacg cggcgtgaga gagcgcatct ccaaagagcg aattgcgtct
     5341 tagtacaaga aacaacccga tcaccgatga tgttatcgcc actgaaacgc ctgcaatgag
     5401 agcgcgctgc atgaacccaa attccaaaac ttcaagaatc atttattatc tctcatcctc
     5461 tgtaagtgtg gttgtgtagg tgcatgtgag cttgcatact cgattctgaa tatgccttga
     5521 ggagatccgg attttcaaag aattcaattg tcttgccgtg gaagaacatt gagcgattga
     5581 tgcatgccac gctggtggca aacctgttaa ctgcatcaag gtcgtgcgat gcccagatta
     5641 tggtaatgtt attctcctga ttcagccttt tcaaaagaga ataaaacttg ttttgcattt
     5701 caagatcgat gcctgtcact ggctcgtcta gtatcaagag cttggggttg ttcaccatcg
     5761 cctttgctat caatacgcgc tgttgctggc cgccagacaa ctcgcccact ctgcgatctt
     5821 tttgtgcaag aagacctgct gtttcaagtg ccaaagctat tttctcttta gatgttttac
     5881 cagtagtggt gactccaagt gacacaattt cttcaactgt tgcggggaag ttctgctcaa
     5941 ttgttttctt ttgtggtatg tatcctatac ttttgaagac tttgttgttc ttcttttcaa
     6001 tgttctctcc aagaattttt attgtgcctg tatagtcatc aagcagacct agcatacagc
     6061 tgaaaagcgt agtcttgcct gctccatttg ggcctattat tccaagtatg tctccctcct
     6121 caattgtaaa cgagatgtta tccagaacca agttaccaga atatgcatat gagagcctgt
     6181 cgatctcgac tatctgcatt ctagaccctc ctttagattt gcaatattct gcttcatctt
     6241 atcaatatat ccaatgcctg ctgcctgttc tcctttgtca ataccttcaa ttgggctcag
     6301 cactaacacc ttgccttccg gaatctcgtt ggcaattgta tctgcaagcc tgctgtcaat
     6361 caaatcttct gaataaatga tattaatgtc aaactcgttt gcaagctcaa ttatctgttg
     6421 cattgtctgc ggcaatattt ccccctccgg cgaagcaccc tggatgctgt gctgcctcag
     6481 gccatacctt tctgaaaagt aagtaaatgc atcatgaaat gctataaagt gtgatttgtc
     6541 acagtcggcg agctctgacc tgataaaggc atcaagcgag tcaagctctg cggtaaatct
     6601 gtcagcattt tgattgtaat aatctgcgtt gaccgggtct gagctggcca tcatattgcg
     6661 gatcttgtct atctgatgct ttgcaagaat tgggtctagc cagatgtgag gattatttac
     6721 tcctccgcca ccactggcat ctccgtgcct gtgctcatcc tctcccacct cccttccttc
     6781 cttataatta aattcgatcc cttcgctagt gttagcaaca actttggctt ctatgtcatc
     6841 tgcccatctt tcaaaacctg caccattaat gaccaacaca tcggccgcgc gtcctcttga
     6901 aacatcctct gctgtaggct cccagtcgtg tggctctacg ccggccggta caaggcttga
     6961 cacttccgcc ctgtctccaa caaccctgga agcaaattcg taaagcggat aaaaggacgc
     7021 aacaactgtg atcttttcct gctcttgtct atcttgcaga gctggcctgc cagtctgtac
     7081 ggtggcgatc attgcaagtg ggattaatgt ggcaatggct gcaagagctg ctctgctctg
     7141 attcatccta ctattttctc catgttatta aaaatatata cctttgttat taaaaaatat
     7201 ggaagctctt ataaataact taataataat atatcccatt ggcgatagtt agtatttcgc
     7261 tcaataacga tatcttgaca gaaatagaca agttgcagaa agcccttggc ttttctggca
     7321 gatccgagat tgtaagagcc ggcatcagaa atcttctggc agaagaaaag gataggcagg
     7381 atttgtatgg agaactcttt gcagtccttt tggcaataca cgatgaaaaa tcagacgatc
     7441 aggtgacaga aatgcagcac gattatgata aactgatcac tacccatata cacaacaaga
     7501 tagatcgaga ccgatgcctt gaaatatttt tgctaaaagg cgaagctgca gagataaaga
     7561 atatgactaa aaaattccaa tcaaacaaaa agatggatca tgtaaagctg atagcaatgt
     7621 aatgtagtag caggataatc gccatgatca cttttttcac aagccccatt gggcttggtc
     7681 atgcaactcg cgacattgcc attgcagagg aactgaaaac tgacatcctg tttgcaagtg
     7741 gtgaaggcgc gtcaatcctg atcgcaagag aagggttcaa taccctagac gtttacaagt
     7801 c
//
LOCUS       DASQTF010000062         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_565509,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000062 DASQTF010000000
VERSION     DASQTF010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565509"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VE308_01555"
     CDS             complement(<1..854)
                     /locus_tag="VE308_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZA99686.1"
                     /translation="MIKEGMKIIFEYEEEKNTGHLQEEDVCIELPSDIVRTELLIPNI
                     NEVDVVRHYTNLSRMNFGIDIGFYPLGSCTMKYNPKVNEDAARAEGFSNLHPLSPEEC
                     AQGSLKLMWELKEYLKDITGMDDFSLQPSAGAQGELLGVMMAKAYFRQKGEKRTKAII
                     PDSAHGTNPATASMCKLGTITIPTDSRGNMDIRKYKDSLSPEVALVMLTNPNTLGLFD
                     ENTIEISDLAHKNGTLMYCDGANMNALLGITRPGDQGFDMIHLNLHKTFSTPHGGGGP
                     GAGVLGVKS"
     gene            complement(847..2106)
                     /gene="gcvPA"
                     /locus_tag="VE308_01560"
     CDS             complement(847..2106)
                     /gene="gcvPA"
                     /locus_tag="VE308_01560"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZA99687.1"
                     /translation="MDYIPATEKDIKEMLNLVGVDSINDLVKEFIPAFTGKLNLPQPK
                     SELELMHHMKGLAQTNKVLRCFVGAGAYNHYIPSAVSHLLARGDLFTGYTPYQAEMSQ
                     GTLQAIYEFQSFICLLTGMDVANASLYDGASALAEAALLSCSYTGRDRIFVKERLHPQ
                     YLKVLQTYCEGAELTLTDRIDNNTACVIAQNPDFYGNVENLYYLAEQTHRAGALFTTC
                     IVEPTSLAILEPPGKYGADIVAGEGQSFGIPLSFGGPYLGFIAVKDFLLKKLPGRICG
                     MTTDSTGNRGFVLTFQAREQHIRRERATSNITTNQALMALAATIYLALMGRSGLTRVA
                     KMSYARAHLLNEKLEKADFRNLNTKPFYNEFIVKVPGSAEAIASRLLKKGILGGFNLG
                     DDNLLICCTEMNSVQDIDDFVSAIAGD"
     gene            complement(2122..2499)
                     /gene="gcvH"
                     /locus_tag="VE308_01565"
     CDS             complement(2122..2499)
                     /gene="gcvH"
                     /locus_tag="VE308_01565"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZA99688.1"
                     /translation="MSELKFTKEHEWIMIKDEVAIIGISDFAQEQLGDIVSIELPKPG
                     GVFRQGQTMAIVDSVKASSDIYAPISGEIIEVNEGLVEKPELINQSPYDSGWIAKIKS
                     SNIEEFENLMTKEKYDRYIGEIK"
BASE COUNT          675 a          659 c          551 g          846 t
ORIGIN      
        1 gatttcaccc caagcacccc tgctccgggg ccgcctccgc cgtgaggggt tgaaaatgtc
       61 ttatgcaggt taagatggat catatcgaag ccctggtccc ctggcctggt gattcctaag
      121 agtgcattca tgtttgcacc atcgcagtac atcagagtcc cattcttgtg cgcaaggtca
      181 ctgatctcga tagtgttctc atcaaaaagt ccaagggtat ttggatttgt tagcataacc
      241 agtgctactt caggactaag cgagtccttg tatttgcgta tgtccatgtt gccgcgtgaa
      301 tccgtaggta tggtgattgt ccccagctta cacattgaag ctgtagcagg atttgtgccg
      361 tgcgctgaat ccggaattat cgcttttgtc cttttttctc ccttctgcct aaagtaggcc
      421 tttgccatca tgactcctaa gagttcaccc tgtgccccag cagaaggctg cagagagaaa
      481 tcatccatcc ccgtgatatc tttcagatac tctttcaact cccacatcaa tttcagtgat
      541 ccttgagcgc actcttcagg agatagagga tgaaggttgc tgaacccttc agctcttgca
      601 gcatcttcgt ttacctttgg attatacttc atggtgcatg agccaagtgg ataaaatcca
      661 atatcaatgc caaaattcat ccttgacagg tttgtgtaat gtctgacaac atctacttcg
      721 tttatgttgg gaatgagcag ctctgttctg acaatgtcgc ttggcagctc gatgcagaca
      781 tcttcttcct gaagatgtcc cgtatttttc tcttcctcat attcaaatat tattttcatt
      841 ccttctttaa tcacctgcta tggcagaaac gaaatcgtcg atatcctgca cagaattcat
      901 ctcagtgcag caaatcaaaa gattgtcatc acccagatta aagcctccca gtattccttt
      961 tttgagcagc cttgaggcaa tggcttctgc agaccctgga accttcacta taaactcatt
     1021 gtagaagggc ttagtgttca aatttctaaa gtcagctttt tccagtttct catttagtag
     1081 gtgcgctcta gcataagaca tttttgcaac ccttgtcaag ccgctcctac ccatgagtgc
     1141 cagatatata gtcgccgcta gcgccattag agcctgattc gttgttatgt tgcttgtggc
     1201 tctctccctt ctaatatgct gctcccgcgc ctgaaaggta agcacaaagc ccctgttgcc
     1261 tgtcgaatcc gtggtcatgc cgcagatcct gcccggaagc ttctttagca agaagtcttt
     1321 gacagctata aatccaagat aaggtccgcc gaagcttaaa ggtattccaa atgactgccc
     1381 ttctcctgcc acaatatctg ccccatattt tccgggaggc tcaagtattg caagtgaagt
     1441 cggctcgaca atacaagtgg taaagagtgc gcccgctcta tgcgtctgtt cggctaagta
     1501 atacaggttt tctacgttac catagaaatc ggggttctgg gctatcacgc aagcagtatt
     1561 attatctatt ctgtcagtga gtgtaagttc agcgccttcg cagtatgtct gcaatacttt
     1621 cagatactgc ggatgtagcc tttcctttac aaaaatacgg tcccgtccag tataagagca
     1681 tgaaagcaag gctgcctcag ccagggcaga tgcgccatcg tataacgacg catttgcaac
     1741 atccattcca gtcagaaggc atatgaagga ttgaaattca tatatggcct gcaaggtccc
     1801 ctggctcatt tctgcttggt agggcgtgta ccctgtaaag agatctcctc ttgccagcag
     1861 gtggctcaca gctgatggga tataatgatt gtaagctcct gcgccgacaa agcatcgtaa
     1921 gaccttgttc gtttgggcaa gacctttcat atgatgcatt aattcaagct cactctttgg
     1981 ttgaggcaga ttcagtttac ctgtgaaagc aggaatgaat tctttgacca gatcatttat
     2041 ggaatccact ccgaccaggt ttagcatttc ttttatgtcc ttttctgttg caggtatgta
     2101 atccatcgat ttctgttgct cttattttat ttcgccaata tatctgtcat acttttcctt
     2161 tgtcattaaa ttttcaaact cttctatgtt tgatgattta atctttgcga tccacccaga
     2221 gtcgtacgga gactggttta tcaactcagg tttttcaacc agcccttcat tgacctctat
     2281 gatctctccg ctgataggag cgtaaatgtc agaagaagcc tttactgaat cgactatggc
     2341 catagtctgt ccttgcctaa agacgccgcc aggttttggc aattctattg aaactatgtc
     2401 gccgagctgt tcctgagcaa agtccgaaat tccaattatt gccacttcat cctttatcat
     2461 aatccactca tgttcctttg taaattttaa ctctgacata atccgttttt ttgcggcaaa
     2521 cataaaacct tatctcttgt cggctctgtg cacacatcat aaaaagattg attgattgat
     2581 tgatgctcat gcaaaaaaat tatcatcgca ctttatttgc gcttgtagaa cctcgtgctt
     2641 gatacctttg cttcatagtg cttacctcca atgtcaattt gaattgattg atcgagcata
     2701 acttctacag gtacaaagca aaaaccaatg g
//
LOCUS       DASQTF010000063         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1306815,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000063 DASQTF010000000
VERSION     DASQTF010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306815"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VE308_01570"
     CDS             <1..1030
                     /locus_tag="VE308_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HZA99689.1"
                     /translation="AFFIIPEDADDLFTLRRIIEKGDRIIADSTRLIKLVKEYGRPDK
                     GERVKVRVTIKVEQSKLDSAVDRLRIAGTIIDTDNEMVPKGTHHSLSIHAGDMATIDK
                     GRRWRDIELRMLKRSVSSSNFILVAIDTQEAAVAKVSGTHVKVIPNIYSGQSGKRYQT
                     KNPDIETYFVDIAKTVSSIIGENDKVIIFGPGESRRRFFNILVSRQYILKDMVQIVDG
                     MDVAGEDGIFVFLRSAAMKQIMSTSKLAEVSSMLDMVMLMVNRGEAKFAMGMNEVSNA
                     AALKAIEAVVFSDSIFKIANEDEIVKLLNLIESYGAKMFAVDSSTDIGLRVSSLGGIV
                     ALLRYAIH"
     gene            complement(986..1339)
                     /gene="cutA"
                     /locus_tag="VE308_01575"
     CDS             complement(986..1339)
                     /gene="cutA"
                     /locus_tag="VE308_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZA99690.1"
                     /translation="MASSGIIILSTFPSEELAAEVANQVVRAKLCACVNFTKIRSIYS
                     WHGKVEDQHEFIVLFKATSKSAKRLKAEIVRLHPYEVPEIVELKMSNVSKPYLSWLAA
                     ESMYRVAKKRHNAAK"
     gene            complement(1369..2415)
                     /locus_tag="VE308_01580"
     CDS             complement(1369..2415)
                     /locus_tag="VE308_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99691.1"
                     /translation="MAILLKPDIARTYPNVISLMEDEPLLDLQPIDIAISNASYVYFA
                     PRIRKVGPDVKSDVMTKFKDAIKALKRGASLIYMLPTGVGGNNENIALIEHVTGMTIE
                     KDVSYYYLPMSAITTASSETLIGSVKSKHDNHISKMLYDPDTRRKLKFVDVNSAELVH
                     IIKTLSHYSGMASILEICKKATDSNIRSELMHGTFADLYIDDVTSGLYDLRIIGSSLD
                     GTGPLMYLVNGSIKSVDGYVKFLIDQIRATLKKRDLKASRTKVAIAWTLDPHEMRGDR
                     IELLSSFETKVKDYISDVERHHGQTFDLYHTDKTTIVIACSKTDFEKVMSKNIANQEF
                     IVIKANPLCETVQE"
     gene            2408..2575
                     /locus_tag="VE308_01585"
     CDS             2408..2575
                     /locus_tag="VE308_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99692.1"
                     /translation="MAILTDSSINETLEPFMAIEEAILYPSVLKPYTATEAVLEEEEE
                     EEEEEEVALKV"
     gene            2664..3698
                     /locus_tag="VE308_01590"
     CDS             2664..3698
                     /locus_tag="VE308_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HZA99693.1"
                     /translation="MKIWFDILTPKQVMFFRRAVNLLHNSGHEVLCTSRKYREAVELA
                     RIKKLDLTFVGSHGGADRYNKLREGARRTYELAEVVKHFGPEVAVTFSSPEGSRIAFG
                     LGIRHIGFNDSPHAEAVAKLTIPLTSNLYCPWVIPRSAWSGYGIAKKNIVHYRALDPA
                     AWLKHHDDDIPEVKQDKKMILLRLEESKASYIADKKVSTTKMIDNFVDELWQSTNIVV
                     LCRYEDQIAEVESRYGNKVQVLRDVVDGTVLIKSTQLFVGAGGTMTAEAALLGKPTIS
                     IAPIQFYVDKYLVKSGLVKRASNSRSLVRLGMKMISDEQYIQTQKKRGARILGRMEDP
                     TERIISALRL"
BASE COUNT         1054 a          790 c          893 g         1019 t
ORIGIN      
        1 tgcttttttt ataattccag aagatgcaga tgacctcttt accttgcgcc gtatcataga
       61 gaagggtgat cgcatcattg cagacagtac tcgtctaatc aagctggtca aggaatatgg
      121 tcggcctgac aagggtgagc gtgtcaaggt gcgtgttacc atcaaagtag agcagagcaa
      181 gcttgactcg gcagtagata ggcttaggat agctggcact atcatagata cagacaatga
      241 gatggtgcca aaagggactc accattcgct ttctatacat gcaggcgaca tggcaacaat
      301 agacaagggg agaaggtggc gggacattga gcttaggatg ttaaaacggt ctgtcagcag
      361 ctccaacttt attctagtgg caatagatac acaggaagca gccgttgcta aggtatctgg
      421 cacacatgtt aaagtgatcc caaacatcta ttctggccag agcggcaagc gctatcagac
      481 gaaaaacccg gacattgaga cctactttgt agacatcgcc aagactgttt cttcaataat
      541 tggtgaaaat gacaaggtga ttatatttgg tccaggggaa agcagacgca ggttcttcaa
      601 tatattggtg agcaggcagt atatcctaaa agacatggtg cagattgtcg atggcatgga
      661 tgttgcaggc gaggatggga tatttgtatt cttgcgttca gcagcaatga agcagatcat
      721 gagcacaagc aagcttgccg aagtgtcttc gatgctagac atggtaatgc tgatggtgaa
      781 tagaggggaa gcaaaatttg cgatggggat gaacgaagta tcaaatgcag ctgcattgaa
      841 ggcaatagag gcggtagtat tctccgattc catcttcaag attgcaaatg aagatgagat
      901 agtcaagcta ctcaatttga ttgaaagcta cggagcaaag atgtttgctg tcgactcatc
      961 gacagatatt ggcctgcgtg tatcgtcact tggcggcatt gtggcgcttc ttcgctacgc
     1021 gatacattga ttctgcagcg agccatgaca gatagggctt cgatacattg ctcattttaa
     1081 gctccactat ctccggcact tcgtatggat gaagccttac aatttcagcc ttgagccttt
     1141 ttgcagattt tgaagttgct ttgaaaagta caatgaattc atgttgatcc tctacctttc
     1201 catgccaaga gtagattgac cggatcttgg taaaattaac acaggcacac aacttggcgc
     1261 ggacgacctg gttggcaacc tctgcagcca actcttcgct tggaaatgtt gacaggatta
     1321 tgattccact gctagccata tttcacatat ggctgataat acaattaatt attcctgcac
     1381 tgtttcgcaa agtgggtttg cctttataac tataaactcc tggtttgcaa tattctttga
     1441 cattactttt tcaaagtctg tcttggagca cgcaatgacg atagttgtct tgtctgtgtg
     1501 atataggtca aatgtctgtc catggtgccg ctctacatcg ctgatatagt ctttgacctt
     1561 tgtctcaaat gaggacagaa gttctatcct gtcgcctctc atttcatgcg ggtcaagcgt
     1621 ccatgctatc gccacttttg ttctgcttgc cttaaggtcg cgctttttca gtgttgcccg
     1681 tatctgatca ataagaaatt tgacgtaccc atctacactc ttaatgctac cattgactag
     1741 atacatcagc ggccctgtcc catcaagcga tgatcctatt attcggaggt catatagccc
     1801 acttgtcacg tcgtcgatat agaggtctgc aaatgtacca tgcatcaatt cactgcggat
     1861 attgctgtca gtggcttttt tgcagatttc aaggatgctg gccatcccag agtagtggct
     1921 caacgttttt attatatgca ccagctcggc tgaattgacg tctacaaact tcaatttgcg
     1981 acgcgtgtcc gggtcataga gcatcttaga aatatgattg tcatgtttag attttaccga
     2041 ccctatcaga gtctcagaac tggctgtggt aatagcactc atggggaggt aataatacga
     2101 tacatccttc tcaatagtca ttcctgtcac gtgctctata agagcaatgt tttcattgtt
     2161 gccacctact cctgtaggaa gcatatagat tagagaggca cccctcttga gagccttgat
     2221 ggcatccttg aactttgtca taacgtcgct tttgacgtct ggaccaacct ttcttattct
     2281 gggagcaaaa tagacgtatg aggcattgct tattgcaatg tcaataggct gtaggtcaag
     2341 gagaggttca tcttccataa gcgagattac atttggatac gtccttgcta tatctggctt
     2401 gagtaaaatg gccatcctga ccgattcatc gattaatgaa accttggagc cgtttatggc
     2461 tattgaagaa gctattctat atccttcagt gctcaagcca tataccgcaa cagaggcagt
     2521 attggaagaa gaagaagaag aagaagaaga agaagaagtg gcactcaaag tgtagagtga
     2581 gctccccctt tcatccctcc gtatatacct cacgtgatct acaaaaggtt tttccaatgg
     2641 ctttccagct acattgtgta tatttgaaga tctggtttga cattttgacg ccaaagcagg
     2701 tgatgttttt taggcgcgcc gttaacttac tgcacaattc gggtcatgaa gttctttgca
     2761 cctcaagaaa atacagagaa gcagttgaac ttgctaggat taaaaagctc gatcttacat
     2821 tcgttggaag ccacggcggg gctgacagat acaataagtt gcgtgaaggc gctcgcagga
     2881 cgtacgagct ggccgaagtt gtaaagcact ttgggccaga agtggcagtc accttttcgt
     2941 caccagaagg ctcacggata gcttttggcc ttggaatcag gcatattggc tttaatgact
     3001 cgcctcacgc agaagctgtt gcaaagctta cgataccctt gacaagcaac ctatattgcc
     3061 cttgggtgat accacgctct gcatggtcag gttacggcat agcaaagaag aacattgtcc
     3121 actacagggc gcttgatccg gcggcgtggc tcaagcacca tgatgatgac ataccggaag
     3181 tcaagcagga taaaaagatg attcttcttc gactcgagga aagcaaggca tcatacattg
     3241 cagacaagaa ggtcagtact acaaaaatga tcgataactt tgtagacgaa ttatggcaat
     3301 caaccaacat tgtagtactc tgcagatatg aagaccagat agcagaagta gagtcacgct
     3361 atggcaacaa ggtgcaggtg ctaagagatg tagttgacgg taccgtgcta ataaaatcta
     3421 cacagctttt tgtgggggcg ggcggcacaa tgactgccga ggcagcgctc ctgggcaagc
     3481 ctacaatatc gatagcacct atccaatttt acgttgacaa ataccttgtc aaatcagggt
     3541 tagttaagag agcaagcaac tccagatcgc ttgtaaggct tggaatgaaa atgatctctg
     3601 acgaacagta catacagact caaaagaaaa ggggagcacg gatccttgga agaatggaag
     3661 accccacaga gaggataatt agtgccttaa gattataagt atgtcccaga tagttgaagt
     3721 tacgcccgga agcccggtgg aggaaaaatt tctttt
//
LOCUS       DASQTF010000064         6389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_935573,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000064 DASQTF010000000
VERSION     DASQTF010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935573"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VE308_01595"
     CDS             complement(<1..894)
                     /locus_tag="VE308_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZA99694.1"
                     /translation="MNKRTDIDSSLLEHFEREIWSKIPHLEEKSGEAKVVNPTPMIDI
                     TEDLKQCARIEYGLDLADKDLRVLGKFDSNLLAGSIKVRPAVRIIYDAIASGKLKSGQ
                     TIIEATSGNFGIALGQIARLGLNVITLVSRKLQEGVFEELRNEKTRIINLDMDICPAP
                     GMKVNANLLAAKATASNIRSQLNEIGFDPAIFDKSRNEVEELLASQDIINLAKLLAKI
                     YGCFCPEQYDNELNIEVHRAVTAVEIDQQLIEQGHSLAEFKVVCTFGTGGTSGGLSRY
                     LMDKYGKKSLHVVFPLSNQDVA"
     gene            complement(1067..1684)
                     /locus_tag="VE308_01600"
     CDS             complement(1067..1684)
                     /locus_tag="VE308_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99695.1"
                     /translation="MQALVLGIANYTALAERIKPEIEKLIGSKVNLNTIVVAIKRFAD
                     VLEKKNLQQQNSSTYLTAAKAKMSLTGSIIDIDFQKENNDDDNDALASVLDELFEQDS
                     RYNLFQTDSHFSLLTEDADEIRRMVADAVQKFDGKIREGLSKITVSLTSDDQSRYHIL
                     SLISNILYNHQIPIHSAFFATNEVVLTLGDKEAAKAYDLIRMKIG"
     gene            1855..2391
                     /gene="hsp20"
                     /locus_tag="VE308_01605"
     CDS             1855..2391
                     /gene="hsp20"
                     /locus_tag="VE308_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp20"
                     /protein_id="HZA99696.1"
                     /translation="MTRDWDIGFGRRMFESIDKDFAEAEEMLNRMFRTVRETSPSDMV
                     VQGPYYYGYQMTVGPDGKPHVREFGNVKPSSRGLVEQSGVREPLVDTAIDERENTLTI
                     TAEMPGVTKQDIKVNVGEEYVSIHAEKGEKKYHTDIPINVTLDDTSAKATYANGILEL
                     KIKLKAPPKPKAKEVKVE"
     gene            2437..4671
                     /locus_tag="VE308_01610"
     CDS             2437..4671
                     /locus_tag="VE308_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HZA99697.1"
                     /translation="MKNFSNNNNSDNNNKVSLKVAETNPKFVGRGMALVDPKVMEEMD
                     LSTGDVLEISGKKKSYVLLWSSQRDDQGKKLIRIDGYTRNNIGVGIDDAVSVRKVNVK
                     KAEQVILAPTEELNIVGLEDYLPELLEGRVVTRGDMIPLNIMGRRIGFAVTSTSPTDA
                     ASLIDSNTEFIIGSIPKAAAKGVPRVSYEDIGGLKNEVQKVREMIELPLRHPEIFDRI
                     GIEAPKGVLLYGPPGTGKTLLAKAVANETNANFYSISGPEIMSKFYGESEERLREVFK
                     EAEENAPSIIFIDEIDSIAPKREEVSGDVEKRVVSQLLTLMDGIQSRGKLVVIGATNR
                     PNAIDPALRRPGRFDREIDIGIPDEQGRLDILLIHTRGMPLTDDVNLESIAKVTHGFV
                     GADLEALSKEAAMRSLRRILPEINLEQPKIPAEILNKIKITKQDFDEALRDVQPSAMR
                     EVLVQRPNVGWEDIGGLQQVKEELAEAIEWPLKHANLFAEANVEPPKGILLYGPPGTG
                     KTMIAKAVAATSEANFISIKGPELISKWVGESEKGVREVFRKARQAAPCVIFFDELDA
                     IAPRRGGPEGDARVTERVISQMLTELDGLEDLKGVVVIGATNRPDIIDEALLRPGRFD
                     RILEVPPPDKEARKHIFQIHTKKKPLESDVNLDKMVEMTQDMTGADIAAVVNAAAMSA
                     IKEHVRQKSGGKVRISMRHFETALNKIKKKSANRIADSPNLPGDGAITASSSPSPPSL
                     HRIS"
BASE COUNT         1924 a         1295 c         1398 g         1772 t
ORIGIN      
        1 tgcaacatct tggttactaa gtggaaagac tacatgtagt gacttcttac catacttgtc
       61 catcaagtat ctgctcaagc cgcctgaggt gccacctgta ccaaaggtgc agacaacttt
      121 gaattcggcc agcgaatggc cctgttcaat caattgttgg tctatctcca cagcagtcac
      181 agctctgtgt acctcaatgt tcagctcgtt gtcgtactgt tccgggcaga aacagccgta
      241 gatcttcgca agaagctttg ccaagtttat tatgtcctga ctcgctagaa gttcttctac
      301 ctcattgcgt gatttatcaa aaatggcagg gtcgaacccg atttcattga gctgtgaccg
      361 aatgtttgat gcagttgctt tagcagccaa aagattcgca ttgaccttca tgcccggtgc
      421 agggcaaatg tccatatcca agtttatgat acgtgttttc tcgtttctca actcctcaaa
      481 tactccttct tgaagcttcc tggatactag ggtaatgaca tttaggccga gtctagcaat
      541 ctgccctaga gcgatcccaa agttacccga ggtcgcttcg attattgtct gtccactctt
      601 aagtttgccc gacgcaatgg catcatagat tatgcgtact gcaggcctta ctttgatcga
      661 acctgcaagc aaatttgaat cgaacttacc taaaactcgc agatctttat cggcaagatc
      721 caagccgtac tcaattcttg cacactgctt caaatcttcc gtaatgtcaa tcataggtgt
      781 tgggttaaca acctttgctt cgcctgattt ttcttctaga tggggaatct tactccatat
      841 ttctcgctca aagtgctcca acagagagct gtcaatgtca gtcctcttat tcattccctg
      901 caattttctc cactctgctt tataatgcgc aaataggttt tataaagata tgatatgata
      961 ggatttctaa cgctttcggc tcgatcgcat ctatatttgc ctatatatat aggcagttta
     1021 gacttgctcc tgataaaccg cttgtaagta ggtattaaaa aatttattaa cctattttca
     1081 ttctgatcaa gtcatatgcc ttggctgcct ctttatcacc caatgtcaat accacttcat
     1141 ttgtagcaaa gaaagcgcta tggattggaa tttgatgatt atacaatata tttgaaataa
     1201 gtgacagtat atggtaccga ctttgatcat cggaggtcaa ggatacagtt atcttggaaa
     1261 gaccttctct tatcttgcca tcaaattttt gtactgcatc agcaaccatt ctcctgatct
     1321 catctgcatc ctctgtaagc agcgaaaaat gactatcagt ttgaaacagg ttatatctgc
     1381 tgtcttgttc aaagagttca tctaaaacgc tggccaaggc atcattgtca tcgtcattat
     1441 tttctttttg aaagtcgatg tctataatac tgcctgtcaa tgacatcttt gcttttgctg
     1501 cagtcaaata tgtactgcta ttttgctgct gcagattctt cttttccagt acatctgcaa
     1561 atctcttgat agcaactaca attgtattca ggtttacttt ggaaccgatc aatttttcaa
     1621 tctccggttt gattctttca gctagtgctg tatagttagc gatgcctagc actaacgctt
     1681 gcatataaac atgatcattt gcgagaactt tccgtatggc ctctggaacg gatattgtct
     1741 ctgagtacat ctttgaacat gtaataaacc tacgccttag taataaatag attgccctgt
     1801 aacgaaaaaa ttatataatg taatatgtat tacatatagc aggtgaataa caaaatgacg
     1861 agagattggg acataggttt cggaaggagg atgtttgaga gcatcgacaa ggactttgcc
     1921 gaagcagagg agatgctgaa cagaatgttt agaactgtca gagaaactag cccatctgac
     1981 atggtcgtcc aaggtcccta ctactacgga taccagatga ccgtaggtcc cgatggcaag
     2041 ccacatgtaa gggaatttgg caatgttaaa ccatcgtcaa gaggattagt ggagcaaagc
     2101 ggtgttagag aacctctagt tgacacggcg atagatgaga gagagaatac gctgactatc
     2161 actgcagaga tgcctggagt tacaaagcaa gacattaagg tcaatgttgg agaagaatac
     2221 gtctctatac atgcagagaa gggagagaag aagtaccaca cagacattcc aataaatgta
     2281 acattagatg acacttctgc aaaagcgacc tatgcaaacg gcattctgga gcttaagatc
     2341 aagctgaaag cgcctccaaa gccaaaggca aaagaagtca aagtggagta aattagtaaa
     2401 gacccacccc atttttattg agaggtgaaa atagaaatga aaaattttag taataataat
     2461 aacagtgaca ataacaataa agtatcacta aaagtagccg agactaaccc aaaatttgtg
     2521 ggtaggggaa tggccttggt cgatccaaaa gtaatggagg agatggatct atcaacagga
     2581 gatgttttag aaatatccgg caaaaagaaa agctatgtgc tgctgtggtc aagtcaacgt
     2641 gacgaccagg gtaaaaaact gatcagaatt gatggataca ctcgcaataa tatcggcgtt
     2701 ggaatcgatg acgccgttag cgtgcgaaaa gtcaatgtga aaaaggctga gcaagtaatt
     2761 ttggcaccta cggaggagct aaacattgtg ggactcgaag actatctccc tgaattgctc
     2821 gagggacggg ttgttacccg gggagatatg attcccctaa atatcatggg cagaagaata
     2881 ggttttgctg ttacaagcac ttcacctact gatgcagcgt cattgattga cagcaatacc
     2941 gaattcatta tcggctccat tcccaaagcg gctgcaaaag gggttcctag ggtgtcctat
     3001 gaggacattg gtggactgaa gaatgaagtg cagaaagtta gggaaatgat tgaactccct
     3061 ctgagacacc cggagatatt tgaccgcata ggcattgaag ctccaaaagg tgtgttgctt
     3121 tatggtccac ccggaacagg gaaaacgcta cttgcaaagg ctgtcgccaa cgaaacaaac
     3181 gccaactttt actctatcag cgggccagaa attatgagca aattctatgg ggaaagtgaa
     3241 gaacgtctaa gagaagtatt caaagaagct gaagaaaatg caccttcaat tatcttcatt
     3301 gatgaaattg actccattgc tccgaaaaga gaggaagttt caggagatgt ggaaaagcgt
     3361 gttgtctcgc agcttcttac attgatggat ggtatacagt caaggggcaa actcgtggtc
     3421 atcggtgcaa caaataggcc aaacgcaatt gaccctgcac ttaggcgtcc tggcaggttt
     3481 gatagagaaa tagatatagg aatacccgac gaacagggtc gattggacat cctgttgatt
     3541 cacactcgtg gcatgccact gacggatgat gtcaacctcg agtcgatcgc aaaggtgacg
     3601 cacggatttg taggtgctga ccttgaagct ttgagcaaag aagcagctat gcgctcacta
     3661 agaaggatac tgcctgaaat taatctagaa cagcccaaga tccccgcgga gatattaaat
     3721 aaaatcaaaa tcacaaagca agactttgac gaagcattaa gagatgtaca gccttctgct
     3781 atgcgggaag tgctcgtaca aaggccaaat gtaggatggg aggatattgg cgggctgcag
     3841 caggtgaagg aagaattggc agaagcgata gaatggcctc tcaaacatgc aaatctattt
     3901 gccgaagcaa acgtagaacc accaaaaggg attctcttgt atggtcctcc tggaactggt
     3961 aagaccatga tagccaaagc tgtcgcggca acatctgaag ctaacttcat cagcatcaaa
     4021 ggacctgaat tgataagcaa atgggttggc gaatcggaga agggtgttag agaagtcttc
     4081 agaaaagctc gtcaggcagc tccctgtgtt atattctttg acgagctcga cgccatagca
     4141 ccgaggaggg gaggtcctga aggcgatgca cgtgttacag agcgagtcat tagtcaaatg
     4201 ctgacagagc tagatggctt ggaagaccta aagggcgtag ttgttattgg tgcaacaaat
     4261 aggccggaca taattgacga agcactcttg cgtcctggaa ggtttgatag aatactggaa
     4321 gtaccgccgc ctgacaagga ggcaaggaag catatattcc aaatacacac aaagaaaaag
     4381 cccctggaat ccgatgtaaa ccttgacaag atggtcgaaa tgacacaaga catgactgga
     4441 gcagacattg cagcagtagt aaacgcagct gccatgagtg caataaagga gcacgtcaga
     4501 caaaaaagtg gtggtaaagt aagaatctca atgagacact tcgaaactgc attgaataag
     4561 attaagaaga agtcggccaa tagaatagct gattctccta accttcctgg agatggcgct
     4621 attactgctt cttcttctcc ttctcctcct tcacttcatc gcatcagctg acgccaccgg
     4681 ctttagaaga aaaaatccaa tgcagaacat gtctatgcta gagagaatct aatcagtttt
     4741 cagctagggg gttgtttgac tttctgaatg gtagcataag gtttgtagga aatgccggta
     4801 ctccagaaca tattctattt ttaaaaacga cctaaagagt ttttcaaaca atcatgtgtt
     4861 tcagatactc ccacagcgat catgcctata tgcaaggaca atggaggagg gacgggtatg
     4921 gcgtagagct gacgaggcca taggatgaaa tctccgacat cagggatcta atttttccta
     4981 agccgtgagc gcagaccgtt aaatgaaatt actagattta tggaaagacc gaaagtcatt
     5041 acctctgaat aaagctattc aatgtaaagc ccatcatgca atcaagcact ttccaaactc
     5101 tagtggcaat ttctcctcga tatagtgctt agtattgcat attgcttgct ctcaagaatg
     5161 atatatatct ccgctcactg ttgaatagtc aattattaaa ggtagcttat ctaccgggag
     5221 atatattatt ctctagctat ttagccatga tcttttcaag ggatcagatt cgatttttaa
     5281 aggaataact agaagactta tgataaaacg ggttcagtgg gattaattcc cgcatgtaaa
     5341 ggggttcgac tcattaagcc ttaaggatta cttatatggc ggataatgtt tgtcactgta
     5401 tcggctgcga gtccatgtgg ttatatgctt ttagaagcac taaagtgaat gcggaaataa
     5461 ggtgtagatt aagaagataa cactggaaac atcagaaacg gcacaatctg gataaagttc
     5521 aactacacat ttactaatcc tttagctagc gaacttgact atactcaaga gggtttaaag
     5581 ttcaaaagta acaggactgt gatattgtca tgattaagct aagtttagct tttttacaca
     5641 atatttttag ttagagcaac agcaacaacc tctctttcta ggtaacccgc ggaagataga
     5701 gatatggctg acaaatattc ctatttttac ccagctctgg cagccatatt attttagcag
     5761 atagaatgcc aacagttcct ttaaaggatt aggtgtcaat agttcaagtt ttaaacaata
     5821 gcaaaaataa tatctctatg agttctgcct gctactttag cttggcttta atcttgtctg
     5881 aggttcgcta ttactgctga attattagtg gtatcagcag tatttgattc attcatattt
     5941 acctctctaa gaggaatttc gctctaatac gtacttactt tctgacctgc ctactgctgc
     6001 tgtatgaagt gagttatcgt atatgtgaga agttctgcca cagccctaaa aagctggctt
     6061 aggtcgtggg ttcaaatccc accaggttca tttcttttat tctggtaaaa ctgcgacata
     6121 gaatggtgcc ttcttcttct tctggttcga tggcttcatc tggtactgta catccagtca
     6181 gggagtgcat cagaagcagc attgtcagtc agccctgtct gattacttcc atcttgtgcg
     6241 ttcattatat agatgtctgt gttgccgtct ctgtcgttag taaaggcata cctatttaga
     6301 caaatgacac aagttgtcat tcgatacatt gctctctgga gctcacaatc cagccttttg
     6361 cctcgtaaat actaccacac tgttcagtt
//
LOCUS       DASQTF010000065         3054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_102251,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000065 DASQTF010000000
VERSION     DASQTF010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102251"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..725
                     /locus_tag="VE308_01615"
     CDS             321..725
                     /locus_tag="VE308_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99698.1"
                     /translation="MKEHAGQQREVITIQYKNNMPLVCVGIIDAGSNKKIVVDAHLDF
                     ASSRTIIPTYIANEMNLSFAGSDETATGAGIIYIPYYEVSIEIFGKIYDNIHVGCLDL
                     PEQTPAKALVGRDILDNYRICLDGERKEIRVC"
     gene            999..1766
                     /locus_tag="VE308_01620"
     CDS             999..1766
                     /locus_tag="VE308_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZA99699.1"
                     /translation="MLKDKVAIVSGASRGLGFVISKEFAERGATVIMCSRSMASAEHS
                     ASLIRGRTHPERLDVTNAQGIAEFMHHMAERHKHIDILLNNAGYPFDRMIWNKRFHQV
                     AEEDLEKVIDVDLKGTFRLSQAAIPFMIKNGSSARGAGGVIINIASTPAIAGHTAGAP
                     YSIAKSGVIAITKHIALEYGDKNIRAYTLALGNISTEATFASMTLTERKKAAMENSMK
                     RWGDPREVATIAANIASEDFAFATGNTIVIDGGTVML"
     gene            1766..2938
                     /locus_tag="VE308_01625"
     CDS             1766..2938
                     /locus_tag="VE308_01625"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HZA99700.1"
                     /translation="MFLAKDKDQDLEKLAEQEQKLPAEIEEKKRKGTLRTGYTTGTTA
                     TAATKGALYALITGRPTEQVTVSLPKDRTATLKIAWTKNEKNGKVTCAAIKDGGDDPD
                     ATHGAEICSTVSFSKNANNIYIDGGKGVGRVTKPGLGLEIGKAAINPTPMKMLEQAVR
                     EVAGHQLEYQGVDVIISVPNGEEIAKKTDNPRLGILGGISILGTTGLVLPYSTASFAA
                     SIRQGLDVATAMGADTVVLTTGGRSEDFAKELFKLPDHCFIQMGDFAGYSIKQCANKM
                     ALRKVIIAGFIGKLTKMAMGIKQTHVAGSHVDMEFMARLAAECEAPLKVVEDIRSANT
                     ARHVSEIIKKNNIVNYYDHLCNKVYQQMHEHVKGQLQIEVMLFEFDGMVIGRHPKK"
BASE COUNT          953 a          612 c          718 g          771 t
ORIGIN      
        1 cgtcgtgctt gcctgccaaa tgaagatgca gaagaacata gttaatttgg ttgagtaagg
       61 gcttgtttga ttcgtcacct ttgcgagctt tttgcttcaa gtcggctgga actgtattcc
      121 accattcttc aaacttgcgt gtcatatatc tcatacatgg atcgggggag tactttaatc
      181 tttcatcttg gtgatgcaat tttttcaacc aaccaaagtt ttgaacccaa aatgaaacca
      241 gcacagagag atatgatata tgaccagcta gttattttag gagaacagga ttatagtctg
      301 tggctgataa tgaattgagt atgaaagaac atgcaggaca acaacgagaa gtcattacaa
      361 tccaatacaa aaacaatatg ccactggtct gtgttggaat catagatgca ggcagcaata
      421 agaaaattgt tgttgatgct cacctcgatt ttgcttcatc aaggacaatt atacccacat
      481 atattgcaaa tgagatgaac ctttcatttg ccggaagcga tgagacagca acaggagcag
      541 ggataatcta tattccgtac tatgaagtct caattgagat ttttggcaaa atctatgata
      601 acatacatgt tggatgcctt gacttgcctg agcagactcc cgccaaggca ctggtcggtc
      661 gcgatatact tgacaattac aggatatgcc ttgatggaga aagaaaggaa atcagagtgt
      721 gctaaaaagg caataggatt gcaggttaac aacaaaccca ttttaaaacc ggctctgttg
      781 ccaacgctct ttagctcgtc gcggcttctt ttaattgtct tgttcatgac ttgattggca
      841 gaggattaga tggctgcttt acaccaaaat acatatatgt gcacgagcgt aacccatgct
      901 actttatctt gtgatgtagg tacaatattg agcaattccc agagtaccat gatttgcact
      961 gataatgtta aaaatatccc aggtagcata aattgccagt gctaaaagac aaggtggcga
     1021 tagtcagcgg tgccagtagg ggtcttggct ttgtgatctc aaaagagttt gctgaaaggg
     1081 gcgccacagt cattatgtgc tcaagaagta tggcaagtgc agaacattct gccagcctga
     1141 taagaggcag gacacaccct gaaagattgg acgtgacaaa tgcccaaggc atagcagaat
     1201 tcatgcacca catggcagag aggcacaagc atattgatat attgctcaat aacgcgggct
     1261 atccctttga tcgcatgata tggaacaaaa gattccatca agttgccgaa gaagatcttg
     1321 aaaaagttat cgatgtagat ctcaaaggta cttttaggct ctcacaagcc gcgattccct
     1381 tcatgataaa aaatggcagt agtgccagag gagctggagg agtgataatc aacattgcct
     1441 ctacaccagc aattgcgggc cacacagcag gtgctccgta tagcattgcg aagtccggag
     1501 ttattgcaat aacaaagcat attgcattag agtatggcga caagaatatc agagcatata
     1561 ctcttgcgct tggcaacatt tctactgagg ctacttttgc ctccatgacc ttgactgaaa
     1621 ggaaaaaggc tgcaatggag aattcaatga agaggtgggg cgacccaaga gaagttgcta
     1681 caattgctgc aaatattgct agcgaagact ttgcatttgc gaccggaaat acgatagtta
     1741 tagatggagg cacagtgatg ctataatgtt tcttgctaaa gataaagacc aagacttaga
     1801 aaaactagca gagcaagaac agaagcttcc ggcagaaata gaggaaaaga agcgtaaggg
     1861 aacacttcga acaggctaca ctactggcac tactgcaact gccgccacaa aaggagcgct
     1921 ttatgctctt atcactggca gaccaacgga gcaggtgact gtatcgttgc caaaggacag
     1981 aacggctaca ctcaagattg cttggacaaa gaacgaaaaa aacggcaagg tcacttgcgc
     2041 agcgataaaa gacggtggcg atgatccaga cgctacacat ggtgcggaga tatgctccac
     2101 cgtgtccttc agcaaaaatg caaataatat ctacatcgat ggcggcaagg gagtaggcag
     2161 agtaacaaag cccggcctag gtcttgagat tggcaaggcc gccataaacc ccacacccat
     2221 gaagatgcta gagcaggcag ttcgcgaagt tgcagggcat cagctggaat accaaggagt
     2281 tgacgtgata atttctgttc caaatggaga agaaattgcc aaaaagactg ataatccccg
     2341 tctcgggatt ctaggtggta tctcgatact tggcactacc ggcctggtcc tgccatactc
     2401 taccgcatct tttgctgctt caataaggca aggccttgat gttgcaactg ctatgggtgc
     2461 tgatactgta gtccttacca caggaggaag gagtgaggac tttgccaagg aattgttcaa
     2521 gttgccagac cactgcttta ttcagatggg tgactttgcc ggatattcaa taaagcaatg
     2581 tgctaacaaa atggccctaa gaaaggtcat tattgccggc tttatcggaa agctgactaa
     2641 aatggccatg ggcatcaaac agactcatgt cgccggctct catgtcgaca tggagttcat
     2701 ggcaaggctc gcagctgaat gtgaagctcc actgaaagtc gtcgaagata ttcgcagtgc
     2761 aaatactgca agacacgtat cagagataat caagaaaaac aatattgtca actactatga
     2821 tcatttatgc aataaggtct atcaacagat gcatgagcat gttaagggcc agttgcaaat
     2881 tgaagttatg ttgtttgaat ttgacgggat ggtaatcggc aggcatccaa aaaaatgact
     2941 gttatgatat tggtttttgt tctgctcttt gaatatcaat gggacaacct tctctttcat
     3001 ataaaacaat gatttgcagt taatgtatca aaagaactgg agggcgtttg taaa
//
LOCUS       DASQTF010000066         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_288628,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000066 DASQTF010000000
VERSION     DASQTF010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288628"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VE308_01630"
     CDS             <1..342
                     /locus_tag="VE308_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99701.1"
                     /translation="DWWPDDEIKLRFVEQSDSYWLIIYGGSTYKGDNTGFVKKVQASD
                     NYKRFKEGYEKKAILRFGIYKENPKKKDDDRKYDLVFLKKSKSIYDIAFLNYDELSPE
                     SIEWQCIEENK"
     gene            315..911
                     /locus_tag="VE308_01635"
     CDS             315..911
                     /locus_tag="VE308_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZA99702.1"
                     /translation="MAVHRGKQMKKKFNCVQDCSDCCIYREYYPSIEYGKIGVLVLPE
                     EKAAIERLANSIDIKVRIIPRLAVGKKSPEKIIAYQMMGKNEDGDLCPFLDVEGRERS
                     AHGGFRCKIYSDRPLACRAYPVIDAVDKSARFDPHCKFCKKFSTTKASSEGLQQEIEA
                     LAKIRACVTADDDVSIWRYATATGRSNGLLSEGWILER"
     gene            complement(908..1126)
                     /locus_tag="VE308_01640"
     CDS             complement(908..1126)
                     /locus_tag="VE308_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HZA99703.1"
                     /translation="MTRKGRLEEIFSKALYADDATLYSVSYRDFENIVEVPLQEFLKL
                     SENFTLIPQNRILLVKKNDQVLYQKYGY"
     gene            1178..1579
                     /locus_tag="VE308_01645"
     CDS             1178..1579
                     /locus_tag="VE308_01645"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HZA99704.1"
                     /translation="MPKKSSSSTIGRRSTTRRGKRTSGRRTTSGTTRGGRSNSGTSSR
                     RGSSSNSSSSSRSSSALSARTRKAIDTLPSHAQKIYSKAHKNALEQYASPSKRRGGKR
                     QSKEQVAHKVAWSAVKKEYKKKGDKWVPKKD"
     gene            complement(1576..>2069)
                     /gene="leuD"
                     /locus_tag="VE308_01650"
     CDS             complement(1576..>2069)
                     /gene="leuD"
                     /locus_tag="VE308_01650"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019913.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZA99705.1"
                     /translation="SIRGKVHKYDINNIDTDVIIPGPYLKIHDHKELAKHAMEGIDPS
                     FPGKVSQGDFLVAGNNFGCGSSREHAPIALSETGIKAILAQSFARIFYRNAVDGGYLL
                     PIEIDESTVKKISDGDELEIDIVKNKITNITKNDHYLMKPFPELIAKIVEAGGLINYK
                     PDY"
BASE COUNT          617 a          422 c          487 g          543 t
ORIGIN      
        1 gattggtggc cagatgatga aataaagctt aggtttgtag agcaaagcga cagctactgg
       61 ctgataattt atggaggctc tacttataaa ggcgacaaca cgggctttgt gaagaaggtg
      121 caagcctctg acaattacaa gcggtttaag gaagggtacg aaaagaaagc aattttgcgt
      181 tttggaatat acaaagaaaa cccaaagaaa aaggacgacg ataggaagta tgaccttgta
      241 tttttaaaga aatcaaagtc tatttatgat attgcattct tgaactatga tgagctttcg
      301 ccggaaagca ttgaatggca gtgcatagag gaaaacaaat gaaaaaaaag ttcaactgtg
      361 tgcaagattg ctctgactgc tgcatctaca gagaatacta tccctctatt gagtatggca
      421 agataggggt acttgtactg cctgaggaga aagctgcaat agagcgcctt gcaaatagta
      481 tcgacatcaa agtcaggata ataccacgcc ttgcagttgg taaaaaatca ccggagaaga
      541 taatcgccta ccagatgatg ggcaagaatg aagatggtga cctctgcccg ttccttgatg
      601 ttgaaggtag ggaaagatct gctcacggtg gatttagatg caaaatatac tctgatcgtc
      661 cgcttgcctg cagggcatac cctgtaattg atgcagtaga caaatcggct agatttgatc
      721 cacattgcaa gttttgcaag aaattctcaa ccacgaaagc tagcagcgaa ggtttgcagc
      781 aagaaattga agcacttgcc aagatcagag cctgtgtgac tgcagacgat gatgtttcta
      841 tttggcgcta tgcaactgct actggcagat caaatggctt gttgtcagaa ggctggattc
      901 tcgaacgcta gtaaccatac ttttggtata gcacctgatc attttttttg acgagcagga
      961 tcctgttctg cggtatcaat gtaaaatttt ccgacaactt gagaaattct tgcagcggga
     1021 cttctacaat attttcaaag tcgcgatagc taacggagta aagtgtcgcg tcatcagcgt
     1081 acagggcctt gctgaaaatt tcctccaacc tgcctttccg cgtcatgaaa tacaatattc
     1141 gtgcatatat tatatattat gttgagccaa tcgcaatatg ccaaaaaaat cttcttcttc
     1201 tactatagga cgtagaagta caacaagaag aggaaagcgc accagcggca gacgaactac
     1261 tagcggtact acgagaggtg ggcggagtaa tagcggtaca agttctaggc gagggagcag
     1321 cagcaatagt agtagtagta gcaggagcag ttcagccctc tctgcaagga cgagaaaggc
     1381 aattgacaca ctcccctctc acgctcagaa gatatacagc aaggctcaca aaaatgccct
     1441 cgaacaatat gcaagccctt caaagaggcg aggaggtaag cgtcaaagca aagagcaggt
     1501 tgcccacaag gtcgcgtggt ctgctgtaaa aaaagaatac aagaaaaagg gcgacaagtg
     1561 ggtgccaaaa aaagattagt agtctggctt gtagtttatt agtccaccgg cctcgacgat
     1621 ttttgctatg agctcaggaa atggcttcat taaatagtgg tcgttctttg tgatgttggt
     1681 tatcttattc ttcactatat ctatctctaa ctcatctcca tccgatattt tcttgactgt
     1741 tgattcgtcg atctctattg ggagcaggta gcctccatcc actgcatttc tataaaatat
     1801 tcttgcgaag ctttgtgcaa ggattgcctt aatccctgtc tcggaaaggg ctataggggc
     1861 atgctcgcgg ctagagccac aaccgaagtt gttgccggcc acgagaaaat caccctgcga
     1921 tactttccca ggaaagctcg ggtcaattcc ctccattgcg tgtttcgcaa gttctttgtg
     1981 gtcatgtatc ttcagataag gccccggaat aatcacatct gtatcaatgt tgtttatgtc
     2041 gtacttgtga accttgcctc ttatgctct
//
LOCUS       DASQTF010000067         3410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1214515,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000067 DASQTF010000000
VERSION     DASQTF010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214515"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1444
                     /gene="sufB"
                     /locus_tag="VE308_01655"
     CDS             41..1444
                     /gene="sufB"
                     /locus_tag="VE308_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZA99706.1"
                     /translation="MSAKEDLNMDYSKYDFRDSTELYVYLSKKGLSRGTVEEISRMKG
                     EPDWMRDFRLRSYEVFLSKPMPKWGGDLSKIDFQNIYYYAKASENSSKSWDDVPESVR
                     NTFEKLGIPEAERKFLAGVGAQYESETVYHNLREDLQKQGVIFSDTDTAVKQYPEIIK
                     KHFGKVIPPEDNKFAALNSAVWSGGSFIYVPPNVKVELPLQAYFRINAENIGQFERTL
                     IIADEGAEVHYIEGCTAPVYTTESLHSAVVELIAKKGAKIRYTTIQNWSKDVYNLVTK
                     RAYAYENAKVEWIDGNLGSKLTMKYPGVYMLGKNAHAEVVSVAFAGKDQHQDAGAKAV
                     HLAPNTTSRITSKSVSKDSGRTTYRGLLHVAKGATGVKSNVRCDALLLDEHSRTDTYP
                     YIEVNEDDATITHEATVGKIGEDQIIYLMSRGYSESDALSLIIGGFMEPFTKELPMEY
                     AVELNRLVKLEMEGSVG"
     gene            1484..2905
                     /locus_tag="VE308_01660"
     CDS             1484..2905
                     /locus_tag="VE308_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HZA99707.1"
                     /translation="MATATLSSINSGHIDLIAAQEPSWLASIRKQAFSQYQSLPSEVS
                     PLYSKYSDVNRIKPESIHFTPQTTRPAALNKDLSDRLKELEQETGILQVGQEINKIFL
                     RDNVAKQGVIISGINDALSSHSDLIRSHVDANQLNYLEDKFLALEGSAFQSGIFVYIP
                     RNVTLEEPIRIITSLADDGTSLISRNIVVADIGSKATVVQEIYAPGSSGRQDVQQGYF
                     ELVETHVNPNAHLELVTLQAMGHDTVNVSNRKAFVERDAKMSWYIGMFGSLLSRYKTD
                     SVMKGPGASSEDVEIVFGIGNQSFDVTSNLLHWGPHTRGRVLVKSVLKDTSKSLFKGM
                     IKIGKEGRGTESYLAGHAILLDRGAKSDAIPGLEIETNEVKATHSASVAQMDENQIYY
                     LTTRGLSREGAKREIVSGFLEPLSRKMGPTIRAWINYLIENKWQGKQLMLRTDEAMEQ
                     ILEVEKSRYRETQDLFEKHYKYR"
     gene            2918..3241
                     /locus_tag="VE308_01665"
     CDS             2918..3241
                     /locus_tag="VE308_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZA99708.1"
                     /translation="MSEQEWVKVCNEKSLKNGDMLDFDQGSKKILIAKIGGNVYATDR
                     ICTHAYADLSTGIINEEEKTVTCPLHLSAFKFDTGIPQNLPAEEPIKTYKVKIQDNAI
                     YIKLD"
     gene            3249..>3410
                     /locus_tag="VE308_01670"
     CDS             3249..>3410
                     /locus_tag="VE308_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZA99709.1"
                     /translation="MQKGALNIEKIRGDFPILKRKVSGGKPLVYLDNAATTQKPLAVI
                     NAISDYYMNY"
BASE COUNT         1066 a          728 c          824 g          792 t
ORIGIN      
        1 gcaaaatgta tataaaacat aacgcagtta tatcaagcgt atgagtgcaa aagaagatct
       61 taacatggat tatagcaaat atgactttag ggattcaaca gagctttatg tttatctcag
      121 caagaagggt ctttctaggg gcacggtcga ggaaattagc aggatgaaag gcgagccaga
      181 ctggatgagg gatttcaggt tgagatctta tgaggtcttt ctgagcaagc ctatgccaaa
      241 gtggggagga gatctgtcta aaatcgactt tcagaatatt tactattatg ccaaagcatc
      301 agagaacagc agcaagagct gggatgatgt tcctgaatca gtaaggaaca cctttgaaaa
      361 gcttggcatc ccagaggctg agcgtaaatt ccttgcagga gtcggtgcac agtatgaatc
      421 tgaaactgtt tatcacaatt taagggagga cctgcaaaag cagggtgtaa ttttctccga
      481 cactgacacg gcagtaaagc aatacccgga aataatcaag aagcattttg gcaaggtaat
      541 cccgccagag gacaacaagt ttgccgccct caatagcgca gtatggtctg gcggctcatt
      601 tatctatgtc ccgccaaacg tcaaagtcga gcttccactg caggcgtatt tcagaataaa
      661 cgcagaaaac attgggcagt ttgagagaac gcttatcatc gcagacgagg gcgctgaagt
      721 gcactatatc gaaggatgca cagcacctgt ctacaccacg gagtcgctcc actctgcagt
      781 tgtggagtta attgccaaga agggcgccaa gatacgctat actacaatcc agaactggag
      841 caaggatgtc tacaaccttg tgacaaagag ggcctatgct tatgaaaacg ccaaggtcga
      901 atggatcgat ggtaatctcg gcagcaaact aacgatgaaa taccctggtg tctacatgct
      961 tgggaagaac gcacatgctg aagtcgtatc ggtggcattt gcagggaagg accagcacca
     1021 agatgcagga gcaaaggcag tccaccttgc gccaaacact acctcgagaa taactagcaa
     1081 gtcggtgagc aaggattctg gcaggacaac ctaccgggga ttgctgcatg ttgcaaaggg
     1141 tgcaacaggc gtcaagtcaa atgtaaggtg cgatgcgcta ctgctagacg agcactctag
     1201 aaccgacaca tatccatata ttgaggtcaa tgaggacgac gcgactatca ctcacgaagc
     1261 aacagtaggc aagataggtg aggaccaaat aatctacttg atgagcagag gatattcaga
     1321 gtcagacgca ctctcgctga tcataggtgg gttcatggag ccgttcacaa aggagcttcc
     1381 aatggagtat gcagtagagc ttaacaggct agtcaagctc gaaatggaag gctcagtcgg
     1441 ttaagccttc actcactcat cctatatctt tcgtgatccc acaatggcga ctgctactct
     1501 atcctcgatt aacagcgggc atattgattt aatagcggcc caagagccca gctggcttgc
     1561 ttcaatacgc aagcaggcct tttcacagta tcagtcactg ccctccgaag tctcgccgct
     1621 ctactccaag tattcagatg tcaacagaat taagccagaa agtatccatt ttactccaca
     1681 gaccacaagg cctgctgcgc tcaacaaaga cctctctgac aggctaaaag agctagagca
     1741 ggagacagga attcttcaag taggtcagga gataaacaag atctttctgc gcgataatgt
     1801 cgccaagcaa ggtgtaatca tatctggtat caacgacgca ctgagtagtc acagcgacct
     1861 catcaggtca cacgtggatg caaatcagct aaattatctc gaggataagt tccttgcact
     1921 tgaggggtcg gcttttcaat ctgggatttt cgtatatatt cctagaaatg tgactcttga
     1981 agagccgata agaataatca cttctcttgc agacgatggc acttcgctga tatcaagaaa
     2041 catcgtcgtt gcagacatcg gctcaaaagc tacagttgtg caggagatat atgcaccggg
     2101 ctcatcaggt agacaagacg tacagcaggg gtattttgaa ttagttgaaa ctcatgtcaa
     2161 tccaaatgca catctggaat tagtgactct acaggcaatg ggtcatgata ctgtcaacgt
     2221 ttcaaacagg aaggcgttcg ttgaaaggga tgccaaaatg tcttggtata tcggcatgtt
     2281 tggatcattg ctttcacgct acaagacaga cagcgtcatg aaagggcctg gcgcctcttc
     2341 agaagatgtt gagatagtct ttgggatagg caaccaatcc tttgatgtta catctaacct
     2401 cctccactgg ggacctcata caagaggaag ggtactcgtc aagtccgtgc tcaaggacac
     2461 ttcaaagtcc ttgttcaagg gcatgattaa gataggcaaa gagggtagag gcacggaatc
     2521 atacctggca gggcatgcaa tccttcttga ccgaggagcc aagtcagatg caatacctgg
     2581 tcttgaaata gaaactaatg aagttaaagc cacacactct gcgtctgtag cccagatgga
     2641 tgaaaatcag atctactatt taacgacaag aggtctgagc agggaaggtg ccaagcgtga
     2701 aattgtatct ggctttcttg agccgctatc tagaaagatg ggtccgacca ttagagcatg
     2761 gatcaactat cttatcgaga acaagtggca aggtaagcag ttaatgctca ggacagatga
     2821 agcaatggaa cagattctgg aagttgaaaa gtcacgctac agggagacgc aagacctctt
     2881 tgaaaagcac tataagtata gatagttgga tggggttatg tcagaacaag aatgggtaaa
     2941 ggtatgcaac gaaaagtcac tcaaaaatgg tgacatgcta gattttgatc aaggcagcaa
     3001 aaaaattctc atagccaaaa taggtggtaa tgtatatgcc actgacagaa tatgtacaca
     3061 cgcctacgcc gatttgtcta ctggaattat taacgaggag gaaaaaacgg tgacatgtcc
     3121 attacacctg tcagctttca agtttgatac tgggattccg cagaacctgc ctgctgaaga
     3181 gccgattaaa acttacaagg ttaaaataca ggacaacgca atttacatca agctggatta
     3241 gcaaaatgat gcaaaaaggg gctctcaata tcgaaaaaat aagaggggac tttcctatcc
     3301 tcaagcgtaa ggttagcgga ggcaagccac ttgtctatct tgacaatgct gccactacac
     3361 aaaaaccact tgcagttatt aatgccatta gcgactacta catgaattac
//
LOCUS       DASQTF010000068         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_566161,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000068 DASQTF010000000
VERSION     DASQTF010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566161"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VE308_01675"
     CDS             <1..1126
                     /locus_tag="VE308_01675"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZA99710.1"
                     /translation="DQVELICNDLARRVFRAEFADCRATSGVVANLAIYAAFSSPGDW
                     MTAASIPSGGHISHGKKEHSGTAGLVHGLNIEHYPFSKEEMTIDVDATKKKIEEMARN
                     GKTPKIGMFGGSLFLFPHPVKELAEFMHDYGMYINYDGAHVAGLIAGGEFQDPLREGA
                     DSITMSSHKTLWGPQGGCIVSYARHAEHIKKAIFPGNTSSHHLHHVAGKAIALAESLE
                     FGKVYAKQVIRNAKALAEALASYGFRLLGEKNGYTASHQIAVDVSKFGDGGTIEEELE
                     KAGIILNRQLLPGDIKAGRHYMHPSGVRIGVPETTRLGMKEGEMKEIANFIKRVVVEK
                     RDPGMIAKDVAEFRKQFQQVQYAFDNAVKAYEYISLTGSR"
     gene            complement(1235..2746)
                     /locus_tag="VE308_01680"
     CDS             complement(1235..2746)
                     /locus_tag="VE308_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZA99711.1"
                     /translation="MKMFPQYLKFTMIPLFLVIVVVAYTLWMLLSDTISQSLDSTIAP
                     SLQEPSYYESNTIIASPSISQNNSSLSSLSVDTMNNDTISVLVGATLIDGTGDPPKPN
                     AVIIINGNKIVAVTNETEYPDQYYSLINNETSRVNILNLTGKYVIPGLFDTHAHVAGV
                     RKNSYNQNFSENALKMLLDYGVTTIRSPAGPTNQSIALKHNVSEGNIEGPEIFTAGRL
                     LNGLQIAIPFVEKQISTEEQAREEVRHQAAVGVDYVKLYVGLPPNLVKAAIDEAHSQG
                     IRVIGHLYMTSWTDAANLGIDALTHGVPVSPFLLPSGDKREQFLENGGGPFDHFLWLD
                     LVDLNSTEVKEMINALVENDIPVDPTLSIYEAMLKDNGYDNGFSGPQNQLRWAKVLQL
                     TKIMYDNGVHILSGSDIPNFGLVPGASLHNELVLLAEAGIKSSEVIQIATNNGAKALG
                     IDDMVGTIQPEKQADIIILSANPIENIRNTKEIEAVIVDGRFADIDNPRFVKN"
     gene            complement(3079..>3342)
                     /locus_tag="VE308_01685"
     CDS             complement(3079..>3342)
                     /locus_tag="VE308_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99712.1"
                     /translation="EEFDTCPKCGIGKMKPTGGAASATSADPDTNRLTGDYYQEYKCD
                     SCGYPEGGQAKAVQVSEQEGIGESMNTSRSSYSDINNKDNQPF"
BASE COUNT          936 a          662 c          754 g          990 t
ORIGIN      
        1 tgaccaagta gagctgatat gcaacgacct tgcacgcagg gtctttagag ctgaattcgc
       61 cgactgcagg gcgacatccg gagtggtagc aaaccttgcc atctatgccg ccttttcaag
      121 ccctggcgac tggatgacgg cggcatctat accgtcaggg ggtcacattt cgcacggtaa
      181 gaaagagcac tcaggcaccg ctggactagt gcatggcctc aacatcgagc actatccctt
      241 ttcaaaagaa gagatgacga tagatgtcga tgcaacaaag aaaaagatag aggagatggc
      301 acgcaatggc aagactccca aaatagggat gtttggtggt agtttgtttt tgttcccgca
      361 cccagttaaa gaattggctg agtttatgca cgactatggc atgtacatca attatgacgg
      421 agcacacgtt gctgggctaa tcgcaggggg ggagttccaa gacccgctaa gggagggggc
      481 tgactcgatt accatgagct cgcataagac gctttgggga ccccaaggcg gttgtatagt
      541 atcatacgcg aggcatgcag agcatatcaa gaaggcaata tttcctggta atactagcag
      601 tcaccacttg caccatgtag caggcaaggc cattgcactg gctgaatcat tagaatttgg
      661 caaagtgtat gccaaacagg tgataaggaa tgcaaaggca ttagcagagg cactcgcaag
      721 ctatggcttt aggcttctag gagaaaagaa cgggtatact gcatcacacc agattgctgt
      781 agatgtgagt aagtttggcg acggcggtac catcgaagaa gagctcgaga aggcagggat
      841 tatattgaat cggcagttac tgccaggtga catcaaggca ggcagacact atatgcaccc
      901 tagcggcgtg aggataggag taccggagac cacgcgcctt ggcatgaagg aaggcgagat
      961 gaaagagatt gctaatttta tcaagcgcgt tgtggtggaa aagcgggatc cggggatgat
     1021 agccaaggac gtggctgagt ttagaaagca gtttcagcag gtgcaatatg catttgacaa
     1081 cgcagtcaag gcgtatgaat atatcagctt gacaggtagt agatagatag aagagacatt
     1141 ctttagaccc aatgcaattc tttcttttta aagctgctga agcacaagtt acagatatag
     1201 tatatatctg atagctgatg acaagaagaa ggcattagtt ttttacaaat cgagggttgt
     1261 ctatatcagc aaatctaccg tcaacgatta ctgcttcaat ctcctttgta ttgcgtatat
     1321 tttctatggg atttgcagaa agaataatga tatcagcttg cttttcaggt tgtatcgtgc
     1381 ccaccatatc atcaatacct agagccttag caccattatt tgttgcaatc tgtataactt
     1441 ccgatgactt tatgccagct tctgctaata gcactagctc attatgaaga cttgctcctg
     1501 gaactagccc aaagttcggt atatcagaac ccgatagaat gtgcactcca ttgtcataca
     1561 ttatttttgt taattgtagt actttagccc aacgtagctg attttgtgga ccagaaaaac
     1621 cattatcata tccattatcc tttagcatag cctcgtatat gctcaaagta ggatctacgg
     1681 gtatgtcatt ttctactaac gcatttatca tttccttaac ctcagtacta ttgagatcca
     1741 ccaaatcaag ccataagaaa tggtcaaaag gacctccacc attttcaagg aattgctcac
     1801 gtttgtcacc tgatggtaat aagaaaggac tcactggcac tccatgagtt aaagcgtcaa
     1861 ttcccaagtt tgcagcatca gtccaacttg tcatatacag atgtcctatg actcttattc
     1921 cttgactatg agcttcgtct atagcagctt ttaccaaatt gggcggtaaa ccaacataca
     1981 gcttgacata atccactcct actgctgctt gatgacggac ctcttctcgg gcttgttctt
     2041 cggtactaat ttgcttttcg acaaacggta ttgctatttg cagaccatta agtaatcttc
     2101 cagcagtaaa tatttcagga ccttctatat tgccttcaga tacattgtgt tttaaggcta
     2161 ttgattgatt ggtaggaccg gcaggacttc ttatggtagt aactccgtaa tctaaaagca
     2221 tcttaagtgc attttctgaa aaattttggt tgtatgaatt ttttctcact ccagcgacat
     2281 gagcatgcgt atcaaaaaga cccggtatga catatttccc ggtaagattt aagatattta
     2341 ctcttgaagt ttcattattg ataagtgaat aatattgatc gggatattca gtttcattgg
     2401 ttactgcaac tattttattg ccattaataa ttatgactgc atttggtttt ggcggatctc
     2461 cagtgccatc tattaaggta gctcctacca atactgatat tgtatcatta ttcattgtat
     2521 ccactgacaa cgatgataat gatgagttat tctgtgaaat ggaaggagag gcaataatag
     2581 tattagattc atagtatgaa ggttcttgta gagagggcgc aattgttgaa tcgagagatt
     2641 gagagatcgt atcagaaagt aacatccata gtgtataggc tacgacgaca ataactaaaa
     2701 acaatggtat cattgtgaat tttaagtatt gaggaaacat tttcatctag ataggtataa
     2761 gagagttaac atttacttaa tgagttcttc ttttttagtc ttgttcttta gttttcaaag
     2821 attaagacaa aattgacctc agtttaagat aacacagagg atctgcttga acctgttctt
     2881 attattggta aaataatata cttatctgga aggataagat aggcgtttct ccgtttagct
     2941 agcagcagcc gccgccgccg atgatgctgc atacatcttg tagtatacta tatgagcttg
     3001 atatccatta caatgttacg gatacgtgtc ttggatatga tctcgccgat gcaatgggta
     3061 ctgaggtagt agaattcgtt aaaagggttg gttgtctttg ttgttgatgt cgctgtagct
     3121 gctgcggctg gtgttcatag attcccctat cccttcttgc tcgctgactt ggactgcttt
     3181 ggcttggccg ccttctggat agccgcagct gtcgcatttg tattcttggt aataatcgcc
     3241 agtcagtcgg tttgtatctg gatctgcgct tgttgctgat gctgctcctc ctgtcggctt
     3301 cattttcccg attccgcatt taggacaagt atcaaattct tc
//
LOCUS       DASQTF010000069         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_935939,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000069 DASQTF010000000
VERSION     DASQTF010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935939"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..2005
                     /locus_tag="VE308_01690"
     CDS             443..2005
                     /locus_tag="VE308_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99713.1"
                     /translation="MEELNLVRQKNGKDKDIGKTLASDRFPNTMDSFWFSINPDIIIS
                     PFDFVTVRHIFNTRTVGMIKELKATEGIGTAARVAVMANTGIENDSVKTISIAMPVGA
                     AKPVRFATQKEVIFALGIPEMQNPIPAGIIEMTNGLQVPVSLDISYLLGPDTAHVNAA
                     GISGNLKTSYLFFLLQSAYQRLVKDMQEDVAIIIFNTKEEDLLHIDQSKKANKKTKQL
                     FDLLELDIKPFENVIYFLPRGRDGRANSAAVPSNSKTYSFGLEDVYDRLDLLFGTNDD
                     SYNVSSILNYIYEAWPLFSGSKRVSTWSDLLEFRGYPQEVVAHKSTLLRFLGHVQRFR
                     KSPMFIDKKVTSKYLGQEIKKIRRSDVFVVDISMIPTLQEQGFVVGDVMKSIDELYSS
                     SRQSGKMPKYILIFVDEINRFIPRSPRNISAVAEQVMRTVVAGKSRGTILFSAQQFKS
                     TVDSALQENTGMHIVAKLGVSELSASPYLMIDQSTKANIVRLNKGELVMVHPAFRQPI
                     KIVFPKAPFKAG"
     gene            complement(2002..2412)
                     /locus_tag="VE308_01695"
     CDS             complement(2002..2412)
                     /locus_tag="VE308_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99714.1"
                     /translation="MKLFFQEFCDKVITLDKSIRFAGIADGDGHLVAIAERKGLKPLL
                     SPEERAQYAITAATRQYTRLRWEYMLGKINYAMSNYAKLVRATIPIADENSRLYYVLL
                     LSFDVEAGRSVHEIITEKIIPLVRKNTNKFLRDA"
BASE COUNT          787 a          551 c          626 g          673 t
ORIGIN      
        1 tgaaccaggc cagacaggaa cagacgccga acgggcccgt gagatagcaa gaagcaaggg
       61 aatggcaaaa ggaacagccg gtgcagctga aacaggcgac cagtacgagc agggtgctgc
      121 tggatcaaat aagtaaacca atcttttttc tttttgtttg tcaacagagt cagagtatta
      181 agatttgatt ctgcagaagc actgactgaa gtaggcagta gtacaagcaa aagatcaaaa
      241 aacttaggaa tattggagtt gagcacacaa agaaatcaaa agtatacata gagattaggt
      301 acaggtagta gttaaaatgg gtcgacaaag acaaggatgg caatcttaat ccagcattaa
      361 atgtgcgctc gcgtatgtga gtatacatga gtatatacgt attacatgat agtcaaatac
      421 gatgtttttc tgttaattta atatggaaga actcaacctt gttcgccaga agaatggcaa
      481 ggataaagat attggtaaga cgctagcatc tgacaggttt cccaacacca tggacagttt
      541 ttggttttcg atcaatcctg acatcattat tagccctttt gactttgtca cagtcagaca
      601 tattttcaat acaaggaccg ttggcatgat taaggagctt aaagctacgg aaggtatcgg
      661 aactgcagcc agggttgcgg taatggcaaa tactgggata gaaaatgact cggtaaaaac
      721 tatttctata gcgatgcctg tcggagcagc caaacctgtc agatttgcca ctcaaaagga
      781 ggtgatattc gcccttggaa taccagagat gcagaatcct atacctgcgg gaataataga
      841 aatgacaaat ggtctccaag tgccagtatc acttgacatt tcatacctgc ttggacctga
      901 caccgcacat gtaaatgcag ccggcatttc aggcaactta aaaacatcgt atctattttt
      961 tctgcttcaa tccgcttatc agagacttgt gaaagacatg caggaggatg tagcgataat
     1021 aatttttaac acaaaggaag aagatcttct tcacattgac caaagcaaga aggctaacaa
     1081 aaagacaaag cagctgttcg acttacttga gctagacatc aagccatttg aaaatgtcat
     1141 atacttcttg ccgaggggaa gagatggtag ggcgaactct gctgctgtgc ctagcaactc
     1201 gaagacatat tcgtttggac ttgaagatgt atacgatagg ctagacctgc tgtttgggac
     1261 caacgacgat agctataacg tttcatcgat actgaactac atatatgagg cgtggccttt
     1321 gttcagcggc agcaagcggg tgagcacgtg gagcgatctg cttgagttca gaggttatcc
     1381 tcaggaggtg gtggcgcaca aatccactct ccttcgcttc ctaggtcacg tccagaggtt
     1441 ccgcaagtca cctatgttta tcgacaagaa agtgacaagc aaatatcttg ggcaagagat
     1501 aaaaaaaatc agacgcagcg acgtttttgt cgttgacata tctatgatac cgacactaca
     1561 ggaacagggg tttgtagtag gagatgtaat gaagagtatt gatgagctgt actcttcttc
     1621 aaggcagagc ggcaaaatgc cgaagtacat cttgattttt gttgacgaaa ttaataggtt
     1681 catcccacgg tctccacgca atatatcggc tgtagccgag caggtaatga ggactgtggt
     1741 cgccggcaag tcacgtggca caatcctgtt ctctgctcag cagttcaaaa gcactgtcga
     1801 ctcagcattg caggaaaaca cgggtatgca catagttgcc aaactaggcg tgtctgaact
     1861 gtcggcgtca ccttatttga tgatagacca aagcacgaag gctaacatag tcaggctcaa
     1921 taagggtgag cttgtgatgg tgcatcctgc ttttcgacag cctatcaaga tcgtatttcc
     1981 aaaagcccca ttcaaggcag gctaggcatc cctcaggaac ttgttcgtgt tcttgcgaac
     2041 aagtggtatt attttttctg ttattatctc gtgcacactc ctccctgcct ctacgtcaaa
     2101 agatagcaga agcacataat agagtcggct gttctcgtca gcaatgggaa tcgtcgccct
     2161 gacaagcttg gcgtagtttg acatcgcgta attgatcttg ccgagcatgt actcccatct
     2221 aaggcgcgta tattgacgag tcgcagcagt gattgcatac tgcgcccgct cctcaggact
     2281 gagcagtggt ttgagaccct tccgttctgc tattgccaca agatggccat ctccatccgc
     2341 aataccggca aacctgatcg acttatcaag ggtaatcacc ttgtcgcaaa attcctgaaa
     2401 gaatagcttc attcagttca tctccaaaag ctgactatat ttgattttcg tacagacgta
     2461 agcaaaatac ttaaatgggt tagacttatc atggtgtctg ctttgtctgc tcaggaagtt
     2521 gcaatgttag aggatcgtag gaactcgcta ttgaacaggc gcgaggtaaa ggtgcttttc
     2581 agaggagtgg ccggcaagat caatagaaat gacgctgctg agaaaatagc taaccag
//
LOCUS       DASQTF010000070         3964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_936016,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000070 DASQTF010000000
VERSION     DASQTF010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936016"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VE308_01700"
     CDS             <1..618
                     /locus_tag="VE308_01700"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZA99715.1"
                     /translation="KIGDKFIAHLQKAEKLSLEAAIKKLEEMTPLAKNPKARSATALA
                     STPDKSAGSKPIAASATATSPTAAIPAAAAPAATVAAGGQTTISLTGTGTSYSKAEGP
                     IDPKFQEKRQVTGTYQGIKVWGPIDPPGQLGIWGQEVCIDFDICVADGACIEACPVNV
                     YEWLETPGHPASDKKAFMIREKDCIFCMACENVCPPQAVKIFKKG"
     gene            662..1198
                     /locus_tag="VE308_01705"
     CDS             662..1198
                     /locus_tag="VE308_01705"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZA99716.1"
                     /translation="MRALEEKFLIVATGGTFDEIHIGHIALLAKAFQVGKKVIIGVSS
                     DKFAANRGKKLNHNFEARIANLKNMIKKEFGNVNYEIAKLDGDFGPAVTTGDVEVLVT
                     SSETQSKGKLLNDMRAKIGVKPVKVVTVELVKAEDGSTISSTRIRTGEIDRQGRLLPR
                     RRSPKAILHELRPANCEE"
     gene            complement(1254..2345)
                     /locus_tag="VE308_01710"
     CDS             complement(1254..2345)
                     /locus_tag="VE308_01710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZA99717.1"
                     /translation="MNVCTSDEQMKREGEEGTPQLRPLLGYWAAQEQYLMQDLLKFVI
                     EAEKGGFTATMTSDHFHPWWHDNAFGNFTWIWIAAAAERTKKMHFTTGVTAPVYRYHP
                     AIIAQAFASLDALYPGRIGLGLGTGEAMNETPLGFEWPSPKVRLARTKEAIDIIKSLW
                     KEEEGKNNENRFVTYNGAYYHIHNAKLYTPPVSHHIPIYLAAAGSQSTKVAAQYSDGL
                     ITYLKPEKAKDVLMRFDKAARASGKNPQSLEKIAEYKVSYSEDFDRAFESTKFWRATL
                     IENVFNSKIADPRKLQQKAKKEVSDEMLKESIQVTSSIEDCTRSIEKYFDAGFTRVYV
                     HSTSPEEVKFVQTFCKKVLPYFMTSKREK"
     gene            2544..3113
                     /locus_tag="VE308_01715"
     CDS             2544..3113
                     /locus_tag="VE308_01715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZA99718.1"
                     /translation="MAQVALFVGSVRRDRQGIKVAHWMEENLTARGHTVYFIDPLELN
                     LPLLDRMYKEMENPPSVLRDLQNKIKNAEGYLAVTPEYNHSTSAAMKNTLDYFLEEYF
                     FKPSAIVSYSTGGFGGVNAAQHLRLIFAELGAPSISSSFAISRVREAFDETGKLIQEQ
                     YNKRITKFLDEFEWYIEAFKNQRLKGVPY"
     gene            complement(3275..>3964)
                     /locus_tag="VE308_01720"
     CDS             complement(3275..>3964)
                     /locus_tag="VE308_01720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZA99719.1"
                     /translation="YKDELPREKYLTWIEEVGIAIKQALTDDGSFFLNIGNKPKDPWI
                     AWDVAYVLGKYFVLQNVIHWVKSIAISKAQVGNYPNIAGDIAVGHFKPIVSKRFLNDC
                     HEYIFHFTKYGDVRLDKLAVGVSYQDKTNIERWKAANKEDRRDRGNIWFIPYQTIQSR
                     SKQRPHPATFPVKLPDMCIRLHGSAKLVIDPFIGIGSTAVAAMRLGISFVGFEIDKAY
                     LDAAIDRTIQV"
BASE COUNT         1123 a          816 c          877 g         1148 t
ORIGIN      
        1 aagataggtg acaagtttat cgcccacttg cagaaggcgg aaaaattgtc acttgaagcc
       61 gcgatcaaga agttggaaga aatgactcca ctggcaaaga accccaaagc tcgaagtgca
      121 actgccctag cctccacacc ggataagtca gctggttcca aacctattgc cgcatcagct
      181 actgcaacct ctccaacagc cgctattcca gcggcagcag caccagcagc aactgttgca
      241 gcaggtggtc aaacgacgat atcacttact ggcacgggaa caagctacag caaagctgaa
      301 ggtccaattg accccaagtt ccaagaaaaa agacaggtaa ctggcaccta tcaaggaatc
      361 aaagtatggg ggccaattga cccaccagga caattgggca tatggggtca agaagtgtgc
      421 atagactttg acatttgcgt tgcagacgga gcatgtattg aagcgtgccc tgtcaatgtc
      481 tacgaatggc ttgaaacgcc aggccatcct gcatctgaca agaaggcttt catgataaga
      541 gaaaaggact gtatattctg tatggcatgt gaaaatgtct gtccgcctca ggcagtaaag
      601 atattcaaaa agggttaggc gggctacagc gaattaatat tgcacgtcac atgactttct
      661 attgcgagcg ttggaagaaa aatttcttat tgttgccaca ggcggcacat ttgatgagat
      721 acacatcggt cacatcgctc tgcttgccaa ggcatttcag gtaggcaaaa aggtgataat
      781 cggggtttca agtgacaagt ttgcagccaa taggggaaag aagctcaacc acaattttga
      841 ggcaaggatt gcaaacctaa agaatatgat caaaaaagaa tttggcaatg tcaactatga
      901 aattgcaaag ttggacggtg acttcggtcc ggcagtaact acaggcgatg tcgaagtgct
      961 agtgacaagc tcagagactc agagcaaggg caagctcctc aacgacatgc gtgcgaaaat
     1021 tggcgtgaag cctgtaaaag tagttaccgt agaattagtc aaagcagagg atggctcgac
     1081 aatttcttcg acccgtatca gaaccggcga aatagacaga cagggaagac tactaccaag
     1141 aagaagatcc ccaaaagcca tcctccacga attacggccg gcgaactgtg aagaataaga
     1201 ggcagagaca tctagagaga ggttcttgat gtctttttcc ttatctcttt ttgttatttt
     1261 tctcttttgg aggtcataaa atatggcaat accttcttgc aaaaggtttg gacgaatttg
     1321 acttcttcag gacttgtgct atgaacataa actctggtaa aacctgcatc aaagtacttc
     1381 tctattgatc gtgtgcaatc ctcaatggaa gaggttactt gaatggattc ttttagcatt
     1441 tcatctgaca cttctttctt ggctttctgt tgtaattttc tcggatcggc tatttttgag
     1501 ttaaatacat tttcaatcaa tgtagcacgc caaaatttag ttgattcaaa tgctctgtca
     1561 aaatcctcag aatatgatac tttgtattct gctattttct ccaaggattg ggggtttttt
     1621 cccgatgcac gggcggcttt atcaaagcgc atcagtacat cttttgcttt ctcaggtttt
     1681 agatacgtga tgagtccatc tgaatattgt gctgcaactt tggtggattg actgccagca
     1741 gctgccagat agattggtat gtggtgactc acaggaggcg tgtatagttt tgcattgtgg
     1801 atgtggtagt aggctccatt gtatgtgaca aatctgtttt cattattttt tccttcttct
     1861 tctttccata atgactttat gatgtcgatc gcttcttttg ttcttgctag tcttactttt
     1921 gggctaggcc actcaaaccc aagcggcgtc tcattcatag cttcaccagt tcctaaacca
     1981 agaccgatcc gtccgggata cagtgcgtca agagaagcaa aagcctgtgc tattattgca
     2041 gggtggtatc tatagacagg ggctgtgacc cctgtagtaa agtgcatctt cttggtacgt
     2101 tctgctgctg cagcaatcca gatccaagta aaattaccaa aagcattgtc atgccaccag
     2161 ggatggaaat gatcgctagt catggtcgca gtgaaccctc ctttctctgc ctcgatgaca
     2221 aattttagca agtcctgcat caaatattgt tcttgggcgg cccagtatcc tagcaatggc
     2281 cgtagttgtg gtgttccttc ttctccttct cttttcattt gctcatcact agtacacacg
     2341 ttcatatcaa atttgtttgc gggatggtat tagtaccccg catttttctc agtggatcct
     2401 tcgctacttg agccatgaga tacatatggc ggactgcata catcactcac atgtataaga
     2461 agagaacttg agttgtatat cactagattc tattcgggtc atttataata tttggagtgc
     2521 actcggttaa tgaattagtg aatatggctc aggtagcgtt atttgttgga tctgttagac
     2581 gcgatagaca aggtatcaaa gtagctcatt ggatggagga aaatttgaca gccagaggac
     2641 atacggtcta ttttatagat cctcttgaat tgaatttgcc attgctcgat agaatgtata
     2701 aggaaatgga gaatccacca agcgtgttaa gagatcttca gaacaaaatt aaaaatgcag
     2761 agggatattt ggcggtgacc ccggaatata atcatagcac atctgcagca atgaaaaaca
     2821 ctttagacta cttcttggaa gaatactttt tcaaaccttc agctattgtt tcatattcaa
     2881 caggaggctt tggcggagtg aatgctgctc aacatttgcg actaatattt gcagaacttg
     2941 gagccccttc aatatcctct tcttttgcaa tatctagagt aagagaggct tttgatgaaa
     3001 caggaaaatt gattcaagaa cagtacaata agaggataac aaagttctta gacgaatttg
     3061 agtggtacat tgaagcattc aaaaatcaaa gattgaaagg ggtgccatac taggatcagg
     3121 attcattgca tgtaagcatc gagatttggt ttgaaacaga tacagatcga aagaaggaac
     3181 atgctgtatg aggaagttca taattctaaa tagcaaaatt tttgcaaatc ttctttttta
     3241 ctctttgaga tttaggtggt ggtaatttat ttaattatac ctgtatggtt ctatctattg
     3301 cagcatcaag atatgcttta tcgatctcaa atccaacaaa tgagattcca aggcgcattg
     3361 ccgcaactgc agtagaacct atgccaatga atgggtcgat gactaatttt gcgctgccat
     3421 gcaaccttat gcacatatct ggaagcttga caggaaatgt tgcagggtgt ggacgctgtt
     3481 tgcttctact ttgtatagtt tgataaggta taaaccaaat gttgcctctg tcccgtctgt
     3541 cttccttatt ggctgccttc cagcgctcaa tatttgtctt atcctgatat gataccccga
     3601 cagccagctt gtccaggcgc acatcgccgt actttgtaaa gtgaaagata tactcatgac
     3661 aatcattgag gaacctctta ctcactatcg gcttgaagtg gccgactgca atgtcaccag
     3721 caatgttcgg gtagttaccg acttgagcct tgcttattgc tatcgatttg acccagtgga
     3781 tgacgttttg cagaacaaag tactttccca gaacatatgc tacatcccat gctatccatg
     3841 ggtcctttgg cttgtttcct atatttagga aaaaagagcc gtcatctgta agggcctgtt
     3901 tgattgcaat gcctacctct tctatccatg tcaggtattt ttcacgaggg agctcatcct
     3961 tgta
//
LOCUS       DASQTF010000071         3165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_380548,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000071 DASQTF010000000
VERSION     DASQTF010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380548"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1201)
                     /locus_tag="VE308_01725"
     CDS             complement(167..1201)
                     /locus_tag="VE308_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99720.1"
                     /translation="MTFDKPSSLPSLARTGSVLSALVIAAFLFAGSLTTVQQTVATTL
                     TEEEQEVLDTQDAATENLSESDRDVNGIEFTPRWGGVSLLQPNDVKVLFADCLEDEFA
                     VSYMSMFESPDVVDYQSFPVAFPDDYMSWLAVVKNTDTNSARVASIGVICADENSGDE
                     DDVNIDINTKTTIQNIVNNFVRVENNQVINLNNLVEVHQTITQNAIQIASITGNNNTV
                     SQVIGQSAQQILNTNTTSPAEVQQIIDQDASQQGVISGGGTLSQAIDQDADQVANVTG
                     GGGNVVDQAIDQDADQVANVTGGGPADVDQAIDQDADQVANVTGGGGNVFDQFLGQDA
                     DQSAQVTDDD"
     gene            complement(1208..1423)
                     /locus_tag="VE308_01730"
     CDS             complement(1208..1423)
                     /locus_tag="VE308_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99721.1"
                     /translation="MQNGLFNSVSHSRYVIIYDRHAGDDINVSHNGYGTFDVDNKRIC
                     GKQLQLNDIRHAASTTTTRNNKWKRRG"
     gene            complement(1886..2866)
                     /locus_tag="VE308_01735"
     CDS             complement(1886..2866)
                     /locus_tag="VE308_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99722.1"
                     /translation="MEDELSIKNPMLLVGIGGVGSKIAAAAGAALECKCLLISNDKKD
                     LVHNNRCTAIFVRSGEWVNPSSYKLRSFVESHRSEMAAAMRGYSTVIVVSNLAGRAGT
                     AIAPLVCRMAKKDSSTAVISITIMPFKYETDRLFSAGTALRRVREGSDSTIVMDNDAF
                     LDNNPELSQQDCFAITNNAIVEVIASVSAGMIKPSMNVLCTSRASPSSESSLRDSLAM
                     LYDAMPDASAVKRAMVYVMGGKRVTIGELNKLVGCVRGIFKEDGSIEVGMLSSMSSSN
                     DGIRVHLVASAPQKTRFDSYDPLGDIIPDVLDWDEPDSAPDIKLTIPAIE"
     gene            complement(2966..>3165)
                     /locus_tag="VE308_01740"
     CDS             complement(2966..>3165)
                     /locus_tag="VE308_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-methylthioadenine synthetase"
                     /protein_id="HZA99723.1"
                     /translation="WQDWQGEIVIDEIAKVRQGRNYAYKPVVISSSSTNAPDRQILLG
                     DKVRVKIYDFSNFSLKAKIIL"
BASE COUNT          791 a          738 c          650 g          986 t
ORIGIN      
        1 ttattcattc taatagaaat aaagaaaaaa gagaaaggga gttttattgc cctcaacttg
       61 gttgccgtga gcaaccaagt caggattgtc tgctttctat agcgttgtct gtctagtcca
      121 ttttacgttc ctgttgttgt ctcgtttgct gatggtgcta tattgtctaa tcatcatcag
      181 tcacttgcgc cgattggtca gcatcttggc ctaagaactg atcaaaaaca ttgccgcctc
      241 cacctgtcac atttgctact tggtcagcat cttggtctat agcttgatca acatcagcgg
      301 ggcctccacc tgtcacattt gctacttggt cagcatcttg gtctatagct tgatcaacaa
      361 cattgccgcc tccacctgtc acatttgcta cttggtcagc atcttggtct atagcttggc
      421 taagagtgcc gccacctgat attactccct gctggcttgc atcttggtcg attatctgct
      481 gaacctctgc aggactggtt gtattcgtat tcaatatctg ctgcgcgctt tggccgatga
      541 cctggctcac agtattgtta ttgccagtga tagatgcaat ttggattgcg ttttgtgtta
      601 tcgtttggtg gacttcgaca aggttgttaa ggttgatgac ctggttattc tctacccgga
      661 caaaattgtt tactatgttc tgaattgttg tcttggtgtt gatgtctata ttgacatcgt
      721 cttcgtcgcc gctgttctcg tctgcacaga taacgcctat tgaagcaacc cttgcgctat
      781 tggtgtcagt attcttgaca actgctagcc acgacatata atcgtcaggg aatgctacgg
      841 ggaatgactg gtagtcaaca acgtctggtg actcgaacat agacatgtag gatactgcaa
      901 actcgtcttc aaggcagtca gcaaataaca cctttacatc atttggttgt agtaaggata
      961 cacctcccca gcgaggggtg aattctatgc cattgacatc tctatctgat tcgctcaggt
     1021 tttctgttgc tgcgtcttga gtgtctaaaa cttcttgttc ttcttctgtg agtgtggttg
     1081 cgacagtctg ttgaactgtt gtcaatgatc ccgcaaacaa aaatgcagca ataaccagtg
     1141 ctgaaagtac tgagcccgtc cttgccaatg acggcaatga tgatggttta tcaaatgtca
     1201 tttgttccta gcccctcctt ttccacttgt tattcctcgt cgttgttgtt gatgctgcgt
     1261 gcctgatgtc attgagttgc agctgttttc cgcaaattct tttgttgtcg acatcaaagg
     1321 tgccatatcc attatggctg acgttgatat cgtcgccggc atggcgatcg tatatgataa
     1381 catacctcga gtgacttacg ctgttaaata agccattctg catatcactc tagaactttt
     1441 ttcatcgtag tccttttcca gcatcacaaa aagcattaaa gaattagttg cccaaatagt
     1501 ttttcgcata acaccttata tcattattgc ggttaatatc ccattattat agaagcaata
     1561 cagtgaatgg ctagacactc agaggatcta ctaatatatc gataaataaa agaggttagt
     1621 ccacagccgt atatatgtac agtcagggaa atttgcaaat ttcgcatttt gagtatgtct
     1681 ccccatctaa gtgattgaaa tgtgtaccgc acgatttgca tgtatggtga atgtcatcat
     1741 aaccgtcttg cgccacaaac tggtaactct taagctgctg actcttacat gctatgcatc
     1801 tgcagccgca cgatatcata tgacaccact agggctctct tactgctcca ctattgctcc
     1861 agatatcttg ctataataat atagcctatt cgatggcagg tatggtcagt ttgatgtcag
     1921 gagccgagtc aggttcatcc cagtcaagta catcgggaat gatgtctccc aatgggtcgt
     1981 agctatcaaa ccgtgtcttt tgtggagctg atgcaacaag gtggacgcgt ataccatcat
     2041 tagaagacga cattgaggat agcatcccaa cttctattga gccgtcctct ttgaatattc
     2101 ctcgcacgca tcctactagc ttgttcagct cgccaatagt gaccctttta cctcccatca
     2161 catacaccat cgctcttttg acagcacttg catctggcat ggcatcatat agcattgcca
     2221 gcgagtcgcg gagtgaggat tccgagctag ggcttgccct gcttgtgcac agcacattca
     2281 ttgacggctt tatcatgcct gcagatactg aagcaatgac ctcaactatt gcattatttg
     2341 ttatggcaaa gcaatcttgt tgagatagct ctgggttatt atctaagaac gcatcgttgt
     2401 ccatcactat cgttgaatct gaaccttcgc gcactcttct cagagctgtt ccagcactga
     2461 aaaggcgatc cgtttcgtac ttgaagggca taattgttat agagattact gcggtactgg
     2521 aatctttttt tgccatcctg catacaagtg gcgctattgc agtgccggca cgccctgcca
     2581 agtttgaaac aacaatgact gttgagtagc cgcgcattgc tgccgccatt tcactgcgat
     2641 ggctctccac aaaagagcgt aatttgtaac tagaagggtt gacccattct ccagaacgaa
     2701 caaaaatagc agtgcaccta ttattgtgga ccaaatcctt cttatcattg cttatcaata
     2761 ggcatttgca ttcaagtgcc gcacccgctg cggctgcaat tttacttcct acgccgccga
     2821 tgcccaccag caacatagga tttttgattg atagctcatc ctccaaggtt tccccgagct
     2881 cccccgcaca aagatacagg tgtcaagtta aaaagctttg gtgattaatc aaaagctaat
     2941 catagcgtaa caaagtttta ctgtactaca aaattatctt tgctttcaat gaaaagttgg
     3001 aaaaatcata aattttgaca cgtactttgt caccaagtaa aatttgacga tcaggagcat
     3061 ttgttgatga tgatgatatg actacaggct tgtatgcata gtttcttccc tgcctcactt
     3121 ttgcaatctc atcaattact atttcaccct gccagtcctg ccaga
//
LOCUS       DASQTF010000072         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_658802,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000072 DASQTF010000000
VERSION     DASQTF010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658802"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VE308_01745"
     CDS             <1..891
                     /locus_tag="VE308_01745"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZA99724.1"
                     /translation="HGCTGKGNDQIRFDVTMRALSPDLKIIAPIRDLNLTRDLEISFA
                     KEQNIPISSEAKKYSIDINLWGRALEGGKIEEPDFEPPEEAFEFINFQNDKAGYIEIE
                     FNQGIPVAADGKHIPLIDLIQYINDKAGGHGVGIIDHIEDRVVGIKSREVYEAPAAVT
                     IIEAHKDLEKMVLTKHELAFKQIVDNQWSWLAYSGLWQDPLRTALDKFIDATQNRVSG
                     KVRLKMEKGSVRVVGRQSEYSLYKDNLATYATGSTFDQSLAKGFVELWGLQSVIANSV
                     TAGLTMDKKNKKEVENEIKS"
     gene            875..1054
                     /gene="lysW/argW"
                     /locus_tag="VE308_01750"
     CDS             875..1054
                     /gene="lysW/argW"
                     /locus_tag="VE308_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-aminoadipate/glutamate carrier protein
                     LysW/ArgW"
                     /protein_id="HZA99725.1"
                     /translation="MKSKVECQECGAEISVPEDTVVGEIITCPDCGGEYEVASKANDK
                     LELKPAERVGEDWGQ"
     gene            1054..1914
                     /gene="lysX"
                     /locus_tag="VE308_01755"
     CDS             1054..1914
                     /gene="lysX"
                     /locus_tag="VE308_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HZA99726.1"
                     /translation="MVEVSIVCDKVRFEEKALYEKTQSKGIKSKIVDAKTITVGTYSK
                     TKDFSLGDVILQRSVSYYRGLYLTACLEFLGFKVINRFEVGQICGNKLITSIKLAENK
                     IPSPKTQFAFSAESGLESIKKTGFPLVLKPIVGSWGRGIYPLRDLETANMIIEMREED
                     NSPLSRIYYIQEMIDRPPRDIRCIVIGDQAVTAIYRYSSQSEWRTNVARGGNVELAPI
                     TKEIEDLALRAAKAVGGGILGVDMMEDKNHGLVVHEINNTVEFRGAATVSKIDIPGEM
                     IDYAVKLARK"
     gene            2032..>2544
                     /locus_tag="VE308_01760"
     CDS             2032..>2544
                     /locus_tag="VE308_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZA99727.1"
                     /translation="MKVGVVGASGYVGGELLRLLVAHPKIELSMVTSRQKAGEYVHRV
                     HPSLKGFVDITFSPMDMEKLTDNCDLVFTSVPHGKANKIVKDLYDRGMKIIDLSADFR
                     LKNPKDYVRWYGWEHPFPEMLSKSVYGVPELHREEIKNAQLVSCPGCMAVTSLLALMP
                     LIANNLIDTDH"
BASE COUNT          813 a          520 c          617 g          594 t
ORIGIN      
        1 catggctgca caggtaaagg caatgatcaa attaggtttg atgttacaat gcgtgcgctt
       61 agcccagacc ttaaaatcat tgctccaata cgcgacctca atctgacacg ggacctcgaa
      121 atatcttttg caaaagagca gaacatacca ataagctccg aggcaaagaa atacagtatt
      181 gacatcaacc tctggggtag agctttggaa ggcggcaaaa tagaagaacc tgactttgaa
      241 cctcctgaag aggcttttga atttatcaat tttcagaatg acaaggctgg ttatatagaa
      301 atcgaattca atcaaggtat cccagtagcg gcggatggca agcacatccc tctcattgac
      361 ctcatccaat acatcaacga taaggctggt ggacatggcg ttggtattat cgaccacatt
      421 gaagatcgtg tagttggaat taagtcacga gaagtgtacg aagccccagc tgcagttaca
      481 ataattgagg ctcacaaaga cctagaaaaa atggtgctta caaagcatga acttgccttc
      541 aagcagatag ttgacaatca atggagctgg cttgcatatt cgggcctatg gcaggatccg
      601 ctgcgcacag ccctggataa gtttatagat gcgacacaaa atcgtgtttc aggaaaagtt
      661 aggctaaaga tggaaaaggg atcggttcga gtggttggca ggcaatcaga atattctcta
      721 tataaggaca atttggcaac atacgccact ggttcaacct ttgatcagag tcttgcaaag
      781 gggttcgtgg agctttgggg gttgcagtca gtcatagcaa attccgtaac tgcaggccta
      841 actatggata aaaaaaataa aaaggaggta gaaaatgaaa tcaaaagttg aatgccaaga
      901 atgcggagct gaaatcagcg ttcctgaaga tacagtggtc ggggagataa taacttgccc
      961 tgactgcggt ggcgagtatg aggtcgcctc aaaggcaaac gataaactgg agctgaagcc
     1021 cgctgaaaga gtaggcgaag attggggaca ataatggtag aggtgagcat cgtatgcgac
     1081 aaagttcgct tcgaggagaa ggcactctat gaaaaaacgc agagtaaggg tatcaagtca
     1141 aagattgtgg atgcaaagac aattactgtc ggtacatata gcaaaaccaa ggatttttca
     1201 ctaggagatg taatactgca gagaagtgta agctattatc gcggtctcta ccttactgct
     1261 tgccttgaat ttcttgggtt caaagtaata aacagatttg aggtgggcca gatttgtgga
     1321 aacaagctta tcacatcgat taaacttgca gaaaacaaaa tcccctctcc aaaaacgcag
     1381 tttgcattta gcgcagagtc tggtctagaa agcatcaaga agactgggtt cccacttgtg
     1441 ctcaaaccga tcgtgggctc atggggaaga gggatatatc ctcttcgtga cctagagact
     1501 gcaaatatga taattgaaat gagagaagaa gataacagtc cactttcgcg catatactat
     1561 attcaagaaa tgattgatag gccccctcgc gatatccggt gcatagtaat cggcgaccaa
     1621 gccgtcactg caatctaccg ctattcttca caatcagaat ggagaacaaa cgttgcccgt
     1681 ggaggtaacg tcgaacttgc cccaataaca aaggagatcg aagatctcgc gctaagagct
     1741 gcaaaagctg taggaggcgg catcttgggc gtcgacatga tggaagacaa gaatcatgga
     1801 ctcgtcgtcc atgaaataaa taacacagtc gagttccgag gcgctgccac cgtctcaaag
     1861 attgatatcc caggcgaaat gatcgactac gccgtcaagc tagcaaggaa ataatgacct
     1921 attctggtca ctattgacct atcatggtta tatctgacca aaaaatataa atgaggattt
     1981 ttgaagcgaa aatttaaaag aaatgcattt tagcaaagga gaatttcaga aatgaaagtt
     2041 ggagtggttg gcgcttccgg atatgtaggg ggagaactat tgcggctttt ggtcgctcac
     2101 ccaaagatag agctcagcat ggtgacatcg cgacagaagg caggcgagta tgtgcaccgt
     2161 gttcacccaa gcttgaaggg ttttgtagac attacattct ctccaatgga catggaaaag
     2221 ctgaccgaca attgcgacct tgtatttaca tcagtaccac acggcaaggc aaacaaaata
     2281 gtcaaggacc tatacgacag gggtatgaaa ataatagacc tgtcagcaga ctttaggctc
     2341 aaaaatccca aggactacgt aaggtggtat ggctgggagc accctttccc tgaaatgcta
     2401 agcaagtctg tatacggagt ccctgagctt caccgggaag agattaaaaa cgcacagctg
     2461 gtctcatgcc ccggttgcat ggcagtaacc tcacttcttg ccctcatgcc actgattgca
     2521 aataatctca tagatactga tcac
//
LOCUS       DASQTF010000073         8149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1029884,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000073 DASQTF010000000
VERSION     DASQTF010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029884"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VE308_01765"
     CDS             <1..202
                     /locus_tag="VE308_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99728.1"
                     /translation="GRYASGQKRCQICEIFIKWDGLWCPCCGYRLRTKPRNLKYKAKL
                     RARNSRTRFETSEPPVLAVRQR"
     gene            complement(203..1069)
                     /locus_tag="VE308_01770"
     CDS             complement(203..1069)
                     /locus_tag="VE308_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99729.1"
                     /translation="MLESIPTAIKVFASEIVYPVALVGCRTSEMSLDCCEYDLAVFAV
                     SQEDNQTNQVVQVDNRTVELMHLTGSIKDHIIDLADMVILKDDSKLMLSSAANDIIVE
                     KHKKMLAASGKKLLISSLFCQHKMRGTKHPMVAAMWLKIAAYDFIDGMLALSGNRPMP
                     VHILEQVRQIDSRMTEGVDVALECIGAERATRPAISRSMEAIKELKSKDYDRELFMSK
                     ISHLLERRMLVDCYFYAGRVAAKNLVSRNEIFYSQYSKLVQLALDLVNDLQSLEKMQK
                     RLFKAANRGLGR"
     gene            1165..2547
                     /locus_tag="VE308_01775"
     CDS             1165..2547
                     /locus_tag="VE308_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZA99730.1"
                     /translation="MKNRSKTHALSANKTNATDADLARRIRSGEFKIAVYGLGHVGSP
                     LASTWLRAGAHLIGVDNSSEVLENARKGRTHVDEPGVNEAYSKGLKEKRFLVYGDPVK
                     ASQDSHFKLICVPVLLTDSFSADLSSVKQVAIAIGQGLKKGDVVSLNPSVPPGTSEDI
                     VLPILEEESGLKVERDFYMIYNPERIYEGRAIEDIEERYPAIVAGAGAKSLEIGAKLY
                     SLVAKKGVIKMGKIRTAETEKLLEGVYRDVNIALANEMAKFCEKAGVDFWEAREAANS
                     QPFCHIHKPGPGVGGACIPVYPQFIIHAANMSGVEFKLVSFGRNVNNSMPAYCVEQAL
                     KLIDRSDLSKCTVALLGLGFRGGVADTRLSPTYKVIEELKKLKVKEIRVHDPLVIEDP
                     NLPHDIMLTSDLSRAANNADLVMLISDHPEYRNITNEDLDNAPVYDGRGILDRSRFAA
                     SRFASIGIPS"
     gene            2585..3628
                     /gene="mtnA"
                     /locus_tag="VE308_01780"
     CDS             2585..3628
                     /gene="mtnA"
                     /locus_tag="VE308_01780"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZA99731.1"
                     /translation="MTHNRDPLLTVAWKNNSVMLIDQTKLPNKLVYVRCRNYEEVADV
                     IRKLVVRGAPAIGVTAAFGLALAAQQSNAKTLPELMTDMDNAFKVLRATRPTAVNLFW
                     ALERVMGKGMHAKSIQEAKSAVLNEALKMAEEDIETNRKIGAHGLKLIKDGDIVLTHC
                     NAGSLATVAYGTALGVIRAARESGKRLSVIATETRPVMQGSRLTAFELLHDGIDVSLI
                     TDTAVGHMMAKGAINHVIVGADRVLHTGHVFNKIGTYQVAILANKHNIPFYVAAPLST
                     FDFESNPDDVIIEDRSVDEVIKVGKKRIAPKGVRVFNPAFDMTPPELITGIITQKGLL
                     KPPFEKNLKAFLG"
     gene            3680..4282
                     /locus_tag="VE308_01785"
     CDS             3680..4282
                     /locus_tag="VE308_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HZA99732.1"
                     /translation="MSTQQTVTEEQVYAALKKCMDPEIPVNVVDLGLIYGVKVAGGKD
                     VDIKMTMTTRGCPLHDTLVSDVKRYVGKVNGVGNVNIDIVWDPPWSLEKMNQDVREKL
                     GFGKPKLRFQIDYEKSKPMKMGRFSKQDDGSLMLANEKDQGFMVNDAIVEFWNACDGT
                     KTINQITDLFSAKLGLPRQQVEQEAVQLLQQLLEAELLKA"
     gene            4257..4856
                     /locus_tag="VE308_01790"
     CDS             4257..4856
                     /locus_tag="VE308_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZA99733.1"
                     /translation="MRLSFSRLNLGGWAFLSFYYPRLVVELGMGDGTLLETLAQSNRN
                     SIYIGIELDDEQCGQARSRITLSNVIIVNRSFEDIVPMFWDNSIDQFVAVLPDPAFVD
                     EKREEQWKPFYKSVYYKLKKGGILRLVTELTDELLQPVSDDRYSAWADWLKSCFISLG
                     FTLAGQQEDAPRQYLSRCIKQFRADPERIRMITLDLLKQ"
     gene            complement(5219..5644)
                     /locus_tag="VE308_01795"
     CDS             complement(5219..5644)
                     /locus_tag="VE308_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99734.1"
                     /translation="MKYHEKGTRNRKNIASNDDVPLEKWLVCYRNPASKKHPDGSIFI
                     RQFYAAGFYEAYDTVMTYAENMNMEVMWFREKRSCEPFLNSDVPELESLCTYCNKKFN
                     DLEPLPCRHEGCKAEFCSRECMANHLALRHKVRDNSACI"
     gene            complement(5807..7279)
                     /locus_tag="VE308_01800"
     CDS             complement(5807..7279)
                     /locus_tag="VE308_01800"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZA99735.1"
                     /translation="MVANYNEVMKLALEQGFYFPGCEIYSDAPAGFWEYGPRGVNMKN
                     KFIELWRRELVRRDGMLEIDGCQIMSKSVFVASGHIGNFTDPVVKCTKCGSSFRADRY
                     ITEKTGENVLERMEVEEIKGLIKKHNLKCPNCNGEFGEVSKFNMMFPVGIGPTAEEAY
                     LRPETCQTIFVDFARIFKTMRGRLPLGIAQVGKSFRNEIAPRQSLLRLREFYQAEIEI
                     FCNPERLNEIPKFEEVKNTVLRLSDGSSIIEVTAEEAISKGLTPNKLITYYLALLVEF
                     YDKTGIDMKRSRFRNLSVDEKAFYASVAFDFEVETSIGWLELVACNYRSDYDLKGHAI
                     TSKQNLEVLDSASQSKVLPHVFELSMGIDRSIYAILEHCYYEDEQHDDRVVLRLRPYL
                     APILVGILPLMTKDGLDQKARQINSELKYDFDTFYDESGSIGRRYRRLEEIGTPFAVT
                     VDKTTMQDDTVTVRHRDTMQQERTTISDLHQYLHKTLGRR"
     gene            7394..>8149
                     /locus_tag="VE308_01805"
     CDS             7394..>8149
                     /locus_tag="VE308_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZA99736.1"
                     /translation="MKVSDRTHGVEYAIRDIITYARKYQASGKEIIYLNIGDPVRFDF
                     KTPDHIKRALVDAVMHDENYYTDSEGLPELRQAIMEKESEKGFNVTVEDIIVTNGVSE
                     GLDMTMASIIDPLAEVLMPGPYYPPYASYAKFYGGKPVEFKLHEDGRPNLEDLRSKIS
                     PRSRALCIISPNNPTGEVFDQKSLQQLIDIAAEYDLYVICDEIYDKIVFDSHFTGIGK
                     VAKDAPVILLNGFSKAYLMTGWRCGYICLNSNSK"
BASE COUNT         2277 a         1839 c         1844 g         2189 t
ORIGIN      
        1 cggcagatat gccagcggac agaaaagatg tcagatatgt gagatattca taaagtggga
       61 tggtctttgg tgtccctgtt gtggatatag gttgaggact aagcctagga acttaaagta
      121 caaggcaaaa ctgagggcga gaaacagccg aactaggttc gagacatctg agccacccgt
      181 cctagcagta agacaaagat aatcagcgtc ctaagcctct gtttgcggct ttaaacagcc
      241 gcttttgcat cttttccagg ctctgcaggt cattgactaa atctaacgca agttgtacaa
      301 gcttcgagta ttggctgtag aatatctcat ttctacttac aagatttttt gctgcaactc
      361 ttcctgcata aaaatagcag tctaccaaca tacggcgctc aagtagatgg cttatctttg
      421 acataaaaag ctctctatca tagtcctttg actttaattc cttgatggcc tccattgacc
      481 tagatatcgc aggtcgggta gccctttctg ctccaataca ctctagcgca acatcaacac
      541 cttctgtcat tctagaatca atttgcctga cctgttccaa aatatgcaca ggcatgggcc
      601 tattccccga cagagcaagc attccatcta tgaagtcgta agctgcaatt tttagccaca
      661 ttgcagcaac cataggatgc ttggttcccc tcattttgtg ctggcaaaac agggaactaa
      721 tcagtaattt cttgcctgaa gcagctaaca tttttttatg tttttccact atgatgtcat
      781 tagctgcaga agacagcatg agcttactgt catccttcaa tatgaccatg tctgccagat
      841 caataatgtg atcttttatt gacccggtga gatgcatcag ctcaactgtg cggttgtcaa
      901 cttgcaccac ttggtttgtt tgattgtctt cttgagatac tgcaaacact gccagatcgt
      961 attcacagca atcaagcgac atctccgatg tacggcagcc gacaagagca acgggataca
     1021 cgatttcact agcaaatacc ttaatggctg taggtattga ttcaagcaat aatgctgcat
     1081 tggctgcttc attaatttaa cctattagct gatatgcaaa agattaactc taatatatga
     1141 acagaaacta tatgttagac tcatatgaag aaccgcagca agacccatgc actctcagct
     1201 aacaagacca acgccacaga tgcagatctt gcaagaagaa tcagatcagg cgaatttaaa
     1261 atcgcggttt acggccttgg acatgtaggc tctcctcttg cctcaacatg gttgcgagct
     1321 ggcgctcatc tgataggcgt agacaactcc tctgaagtgc ttgaaaatgc aaggaagggg
     1381 aggacacacg tcgatgagcc aggagtaaac gaggcctatt caaaagggct caaggaaaaa
     1441 cggtttttgg tctacggcga ccccgtcaag gcttctcaag acagccattt caagttgata
     1501 tgcgtgccag tgctccttac tgattcattt tctgcagacc tttcctcggt aaagcaagtg
     1561 gcgattgcca ttggccaagg cctgaagaaa ggagatgttg tttcactcaa tccaagtgtg
     1621 ccacctggca catctgaaga tattgtcctc ccgatcttag aggaagagag tgggctgaaa
     1681 gtcgaacggg atttttacat gatatataat ccagagcgca tctatgaagg cagggcgatt
     1741 gaagatattg aagagagata tccagcaatc gtcgcaggcg cgggtgccaa gagcttagag
     1801 attggtgcca agctctattc cttggtggcc aagaagggcg tcataaagat gggtaagatc
     1861 aggaccgcag agactgaaaa gctcctagaa ggagtctacc gagatgtcaa catcgctctt
     1921 gcaaacgaga tggccaaatt ctgtgaaaaa gcaggtgttg acttttggga agcaagagaa
     1981 gctgccaatt ctcagccttt ctgccacata cataagcccg gaccgggtgt aggtggcgca
     2041 tgcataccag tttatcctca attcataatc cacgcagcaa acatgagcgg ggttgagttc
     2101 aagctcgtaa gctttggaag gaatgtcaac aattcgatgc ctgcctattg cgtcgagcag
     2161 gcattgaaat tgattgacag gtctgatctt tcgaagtgta cggtggcatt acttggactg
     2221 ggattcagag gtggtgtagc agatacgcgc ctgtcgccca cttataaggt cattgaagaa
     2281 ctgaagaagt tgaaggtgaa agagatccgt gtgcatgacc cgctcgtaat agaagaccct
     2341 aatctaccac acgatatcat gctaacatcg gacttgtcaa gagcggcaaa taatgcagat
     2401 cttgttatgt tgatttcaga ccatccagaa tatcgcaaca ttacaaatga agatctggac
     2461 aatgcaccag tctacgatgg tcgtgggatc cttgacagat cacgatttgc tgccagcagg
     2521 tttgcatcaa taggcatccc tagttagatt taatactgct tgaaaaaaga ttcaataata
     2581 tttgatgact cataatcgag atcctttgct gacagttgca tggaaaaata attctgtgat
     2641 gctcatcgat cagacaaagc ttccaaacaa gctcgtgtat gtaagatgca gaaactacga
     2701 ggaagtagca gatgtaatca gaaaacttgt agtgaggggc gcaccagcga taggcgtaac
     2761 cgctgctttc ggtcttgccc tagcagccca gcagagcaat gccaagacat tgcctgaatt
     2821 aatgactgat atggacaatg cattcaaggt gctccgggcg acgcgaccta ctgccgttaa
     2881 cctcttttgg gcattagagc gggtgatggg aaaaggcatg catgccaaga gcatacaaga
     2941 agcgaaaagt gcagtgctta acgaggcact caagatggct gaagaggaca ttgaaaccaa
     3001 taggaaaatc ggagcacatg gcctcaagtt gatcaaagat ggcgatattg tgctcaccca
     3061 ctgtaatgcc ggctcgcttg cgacagttgc ttatggtact gcccttggtg tcataagagc
     3121 tgctagagaa tctggcaaac gcctgagcgt aattgcgaca gaaacgcgtc cagtgatgca
     3181 gggttctcgt ctcactgcgt ttgagctcct gcatgacggc atcgatgtca gcctgataac
     3241 cgacacggct gtcggccaca tgatggcgaa gggtgcaatc aatcacgtta tcgtaggcgc
     3301 tgacagggtg cttcacacag gccatgtgtt caataagatt gggacatacc aagtggcaat
     3361 tcttgccaac aaacataata tcccgtttta cgttgcagca ccgttatcaa catttgattt
     3421 tgaaagtaat cctgatgatg tcataattga agacagatct gtagacgaag tgatcaaggt
     3481 aggcaagaag aggatcgcgc caaaaggagt gagggtcttc aaccctgcct ttgacatgac
     3541 gccaccggag cttatcacag gcattattac tcagaaaggc ctgctcaagc cgccctttga
     3601 gaagaatctt aaagcctttc ttggctgata aagtttttat catgttttgt ccaacccata
     3661 tacaacagta ggtagagcca tgtcaacaca gcaaacagtc actgaagagc aagtttatgc
     3721 agcactgaaa aagtgcatgg acccagagat acctgtcaat gtagtagatc ttggactcat
     3781 ctatggagtc aaggttgctg gcggtaaaga cgtagacatc aagatgacaa tgactaccag
     3841 aggctgtccg cttcatgata ctctagtaag tgatgtcaag cgctacgtag gcaaggtaaa
     3901 cggagtcggc aatgtcaata ttgacatagt atgggatcct ccttggagtc ttgaaaaaat
     3961 gaatcaggat gtgcgggaga agcttggctt tggtaagcca aagcttcgat tccagatcga
     4021 ctatgaaaag agcaagccca tgaaaatggg tagattttca aagcaggatg acggatcact
     4081 gatgcttgca aacgaaaaag atcaggggtt catggtaaat gatgcaattg tagaattctg
     4141 gaatgcctgc gacggaacca agaccatcaa ccaaatcacc gacctattct cagccaagct
     4201 tggcttacca agacagcagg ttgagcaaga agcggttcag cttctgcagc agttgcttga
     4261 ggctgagctt ctcaaggctt aaccttggag gatgggcctt tctctccttt tattatccta
     4321 ggttggttgt ggaacttggc atgggcgatg gcacattgct tgaaaccctt gcgcaaagca
     4381 atcgcaattc aatatatatt ggaattgagc ttgacgatga gcagtgcgga caagctcggt
     4441 caagaatcac cctttccaat gtaattattg tgaatagatc ttttgaagat attgtaccga
     4501 tgttctggga taattctatt gatcaattcg tagcagtgct gcctgacccc gcattcgttg
     4561 acgagaaaag ggaagagcag tggaaaccgt tctataaatc tgtatactac aagctaaaaa
     4621 aaggggggat cctccggctt gttacagaat taactgatga gttattgcag cctgtaagcg
     4681 atgatcgcta ttcggcatgg gctgattggc ttaagtcctg cttcatttcg cttggtttta
     4741 cattagcagg tcaacaagaa gatgcaccaa gacaatacct atcacgctgt atcaagcagt
     4801 tcagagcaga tcccgagcgc atacgcatga tcacccttga tctgctcaag cagtgatgaa
     4861 atttcagttt cagggccttc atgaacccga ttctaacctg ctaaagatgg ccttatgatg
     4921 gaatgccaca tcagttgtga tgcaaatttc ttttttggcg atcgtatttg tatcctttgt
     4981 tgctagccat tgctgcgcaa ccggaaagaa cctcgccgag gctagagata acctatcgtc
     5041 agataataaa agccaccact tctcgctcta ttagtatagg ccataactgc aggtcagggg
     5101 gtggaagtcg acggatcaat gataatatga cctgtttatg cagcttgttg gtataggccg
     5161 gtattcttac aatagagtgg attccgcccg ccttacaaat gctatcgaga acaacaggct
     5221 aaatacacgc cgaattatct ctaactttat gccgcaatgc aagatgattt gccatgcact
     5281 cccttgagca aaactcggcc ttacagccct catgcctgca gggcagtggt tcgaggtcat
     5341 tgaacttttt gttgcagtac gtgcaaagcg attcaagctc tggaacgtca gagttaagaa
     5401 atggttcgca ggaacgcttt tcgcggaacc acattacctc catgttcata ttttcagcgt
     5461 acgtcataac tgtatcataa gcttcgtaaa agccggctgc atagaattga cgaataaata
     5521 tagagccatc tggatgcttt tttgatgcag gattccgata gcatacaagc cacttttcta
     5581 aaggcacatc gtcattggat gctatatttt tcctatttct cgtccccttt tcatggtact
     5641 tcatgtatgt taccttttaa tttctttcga tcctagttga tcatcaattt tcttcgatca
     5701 gatatgcttt acaaaaaagg aacttctatt accttgagca aaattagagg ctatagtaaa
     5761 aatttcattt acctttgcta aaggaatatc aaggacgtct atgaatctag cggcgtccaa
     5821 gtgtcttatg caaatattga tgaagatcag atatggtggt cctttcttgt tgcattgtgt
     5881 ctctgtgtct tacggtcaca gtatcatcct gcattgtcgt cttgtctaca gttacagcaa
     5941 atggtgtccc tatctcctcg agccttcggt agcgcctgcc aatagaccct gattcatcgt
     6001 aaaaggtatc aaagtcatat tttaactcag aattgatctg acgcgctttt tggtcaagcc
     6061 catctttggt cattagaggc agtataccaa ctaaaattgg cgcaaggtaa ggccttaggc
     6121 gaagcactac tctatcatca tgctgttcat cttcatagta acagtgttcc aaaattgcgt
     6181 agatgcttct gtcgatcccc atcgacagct caaacacatg cggcagcacc ttgctttggg
     6241 atgcagagtc taatacttcg aggttctgct tgcttgtgat cgcatgccct tttaggtcat
     6301 agtcagatcg gtagttacat gccacaagct caagccatcc aatgctggtc tcgacttcaa
     6361 aatcaaatgc gacagaggca tagaaggcct tttcatcgac tgataggttc ctgaaacggc
     6421 tacgcttcat atctatgcca gtcttgtcat aaaattccac gagaagtgct aggtaatatg
     6481 tgataagctt gttcggtgtc aaccccttac tgatcgcctc ctccgcagtt acttcaatta
     6541 tcgacgatcc atccgatagt cgaagcacgg tattcttgac ttcttcgaac tttggtattt
     6601 cattcagtct ttcagggttg caaaagatct ctatctctgc ttggtaaaac tcccttaagc
     6661 ggagaaggct ctgcctaggt gcaatttcgt ttctaaaact cttgccaacc tgtgctatgc
     6721 caagtggaag gcgacccctc attgtcttaa agatacgtgc aaagtcgaca aaaatcgtct
     6781 ggcaggtttc cggacggagg tatgcttcct ctgccgtagg gccgatgcca acagggaaca
     6841 tcatgttaaa cttacttacc tcaccaaact cgccgttgca atttgggcat ttcaggttgt
     6901 gcttttttat gagcccttta atctcttcta cttccatcct ctctagtaca ttctcacctg
     6961 ttttctctgt gatgtaccgg tctgccctga aagaagatcc acactttgtg cattttacta
     7021 cagggtcggt aaagtttcca atatgacctg atgcaacaaa gacgctcttg ctcattatct
     7081 ggcatccgtc aatctctagc atgccatcac gccgtacaag ctcgcgccgc catagctcta
     7141 taaacttgtt tttcatgttg acgcctcttg gcccgtattc ccaaaagcca gcaggcgcat
     7201 ctgagtaaat ctcgcaacct gggaaataga atccttgttc cagtgccaat ttcattactt
     7261 cattataatt agccaccatc tgcaaggagc agacaactca gagaggcttt aaatttatgc
     7321 ctaagttctt gcaaagagta tcatttgtga agacataaca atttatctcg gtcacattcc
     7381 cctaggtttc aggttgaagg tttcagatag gacacacggg gtggagtacg ctatccgcga
     7441 catcatcact tatgcgcgca aataccaggc atccgggaag gagataatct acctcaacat
     7501 tggagatcct gttaggttcg acttcaagac gcccgaccac atcaaaagag cccttgtaga
     7561 tgcggtcatg cacgatgaga attattacac cgattctgaa gggctgcccg agctccggca
     7621 agcgatcatg gaaaaagagt ctgaaaaagg gttcaacgtt acagtagaag acatcattgt
     7681 caccaacgga gtatcagaag ggcttgacat gacgatggct tctatcattg accccctcgc
     7741 tgaagtgtta atgccgggtc cctattatcc cccgtatgct tcatacgcaa aattttatgg
     7801 tggcaagcct gttgaattca agcttcatga agacggaaga ccaaaccttg aagaccttcg
     7861 tagcaagata agtccgcgct cgcgtgcgct gtgtatcatt agtccaaaca accccacagg
     7921 tgaagtattc gatcaaaaga gccttcaaca gttgatagac attgcagccg aatatgatct
     7981 ctatgttatt tgcgatgaaa tatacgacaa aatcgttttt gactcccatt tcaccggcat
     8041 aggcaaggta gccaaagatg cccccgttat cttgctgaac ggcttttcaa aggcctatct
     8101 gatgactggt tggcgctgcg gctatatttg tctgaatagc aactccaaa
//
LOCUS       DASQTF010000074         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1030120,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000074 DASQTF010000000
VERSION     DASQTF010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030120"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1300
                     /locus_tag="VE308_01810"
     CDS             113..1300
                     /locus_tag="VE308_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZA99737.1"
                     /translation="MSTFQKVANRKDLKEGSLLRVGVEGKSIVLAVVLGKVYAMDAVC
                     SHEGGPLEDGSLEGYELKCPWHYALFDVRNAKVSEQTVWATDMQSYSVKIDEASGDIM
                     VSPEPKSPETTIQKESSTTTTTVPKEANKQSGSYSGLTLTKKEELEGTDIRTFKFAKN
                     EYPEYKAGQFAFFPLDGVNNDSRGPVRHFSLASSPTEDALIISTRIRDSPYKQRLSTV
                     QEDEKVKVSKAQGNFILHDDYSKPAIFLSGGIGVTPFRSMIKYATDKQLPLKITIFDS
                     NRNQRNILYKDEFDKWAAQNQNLKIVYTITEEEKGKEQEQHRREDATSSAIEAKGNWN
                     GERGRIDRTMIERHVSKEEISNAIFYICGPPGMINALEQDLLQKQFQIPEGRIKVEEF
                     TGY"
     gene            1385..>2313
                     /locus_tag="VE308_01815"
     CDS             1385..>2313
                     /locus_tag="VE308_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HZA99738.1"
                     /translation="MKSARIVKPNKPLEVQELETPKPRGSQVLIKVESSGVCHSDIHL
                     WEGGYEGLEGQFLKTTDRGVKYPLTPGHEIAGIVDSFGEEAEGFIKNERVLVYPWVGE
                     GRCPACRIGQENLCDKPRSLGVYTDGGYAEYVLIPSYKYLLKMGDGLDANASATLSCS
                     ALTAYGAVKNADLKPNDNVVIVGAGGLGLMAIQLAKAVTGSRIIAMDLNDEKLGAAKK
                     NGADNTINSKQEDPVKAVMELTDKMGADAVIDFVNASKTVESDIQFLRRRAKLVLVGL
                     FGGELKLNLVSMPTRAYRLIGSYTGTLSEMIELV"
BASE COUNT          787 a          409 c          556 g          561 t
ORIGIN      
        1 gactatgcta ccgatgtgac tacatttgac gcattgtaca agcacgcaat atctatggga
       61 gatcagttca cagttggaat tgttaagcag ttccaggagg agtttgtttg aaatgtcaac
      121 ttttcaaaaa gttgctaaca gaaaagacct caaagaaggc agcctactta gagtaggcgt
      181 agaaggcaag tccattgttc tagcggttgt actggggaag gtctatgcaa tggatgccgt
      241 ttgctcgcac gaaggagggc cgcttgagga tggttcattg gagggatacg agctgaaatg
      301 tccgtggcac tacgctctct ttgatgtacg aaatgcaaaa gtttcagaac agactgtatg
      361 ggcaactgac atgcagtcat actctgtcaa gattgatgaa gccagcggcg atattatggt
      421 gagcccagaa ccaaagtcgc cggaaacaac aatacaaaag gaaagtagca ctactactac
      481 taccgttcct aaagaagcga acaaacaatc aggttcatat tctggcttga ctttgaccaa
      541 gaaggaagaa cttgaaggaa cagacatcag gactttcaag ttcgcaaaga acgagtatcc
      601 cgaatataaa gcaggccagt ttgctttctt tccacttgat ggcgtcaata atgatagcag
      661 gggcccagta aggcatttct cgcttgcttc atcacctacg gaagacgccc tcatcataag
      721 cacaagaata cgtgattcgc catacaagca gcggctgtct actgtccaag aagatgaaaa
      781 agttaaagtc tcaaaggcac agggcaattt catattgcat gatgactact caaagcctgc
      841 aatatttctg tccggtggca taggtgttac tccatttaga agtatgataa agtatgctac
      901 agataagcag ctgccactta aaattacgat atttgactcc aatagaaacc aacgaaatat
      961 cttgtacaaa gatgaatttg ataaatgggc tgctcaaaat cagaatctca aaatcgttta
     1021 tactataacg gaggaagaaa aaggcaagga gcaggagcaa cacagaaggg aagacgccac
     1081 cagtagtgcc atagaagcaa aaggaaattg gaatggagaa cgtggcagaa tagacagaac
     1141 tatgatcgaa agacacgtga gcaaagagga aattagtaat gcaatattct acatttgtgg
     1201 acctcctggc atgataaatg ctctagaaca agatttgctg caaaaacagt tccagattcc
     1261 agagggaagg atcaaagttg aagagtttac tggatattaa gccccgaact gcacacagag
     1321 ctcttcgtcg aagtcttgcc ctgctgtcag aaagaaataa ctataacaac tggggaatgt
     1381 tgacatgaaa tctgcaagaa ttgtaaagcc aaacaagcct ttagaagtac aagaacttga
     1441 aactccaaag cctaggggtt cacaggttct catcaaagtt gaatcatcag gtgtttgcca
     1501 tagcgatatt catttatggg aaggaggtta cgagggtctt gaaggacaat ttctaaaaac
     1561 tactgataga ggagtaaagt atccattaac tcctggacat gagatagcag gaatagtaga
     1621 tagttttggt gaagaagcag aaggatttat caagaatgaa agagttcttg tctacccgtg
     1681 ggtaggtgaa gggcggtgtc cggcgtgcag aattgggcaa gagaacctat gcgataaacc
     1741 tagatcattg ggtgtctata ccgatggagg ttatgcagag tatgtactaa taccaagtta
     1801 caaatatcta ttgaaaatgg gcgatgggtt ggatgctaat gcaagcgcca cattatcgtg
     1861 ttctgcactt acagcatatg gggcagtaaa gaatgctgat ctaaaaccaa atgataatgt
     1921 ggttattgtt ggtgcaggag gattaggatt gatggctata caactagcaa aagcagttac
     1981 gggctccaga ataattgcca tggatttaaa tgatgaaaaa ttaggagctg caaaaaagaa
     2041 tggtgcagat aacactataa actcaaaaca agaagatcca gtaaaagcag tcatggagtt
     2101 gactgataaa atgggcgccg atgcagtgat cgactttgtc aacgcttcaa agactgtaga
     2161 gtccgatatt caatttctta gaagaagggc gaagttagtt ttggtaggac tctttggagg
     2221 cgaattaaaa ttaaatttag taagtatgcc aacaagagca tacagattaa tcggatcata
     2281 tactggaaca ctaagtgaga tgatagaatt ggt
//
LOCUS       DASQTF010000075         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1215604,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000075 DASQTF010000000
VERSION     DASQTF010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215604"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VE308_01820"
     CDS             complement(<1..282)
                     /locus_tag="VE308_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99739.1"
                     /translation="MDPVNSKPISWFGDFIGVGYRYHDIYMNVIPLFEEKVSAYRLWK
                     KTIDWWPDDEIKLRFVEQSDSYWLIIYGGSTYKGDNTGFVKKVQASDNYK"
     gene            complement(845..1135)
                     /locus_tag="VE308_01825"
     CDS             complement(845..1135)
                     /locus_tag="VE308_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99740.1"
                     /translation="MSFFGPPSSPSYDKKKYEHLTLAIRQTVLERAHNRCQKCSVKFK
                     PSTPPQFQHINGSLKDNRPVNLRALCSECFKLVEDNEKNRKGIFGRMRNIVG"
     gene            complement(1358..1864)
                     /locus_tag="VE308_01830"
     CDS             complement(1358..1864)
                     /locus_tag="VE308_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99741.1"
                     /translation="MFRSRGGIKSGQYVFVAQKEGKYNKIIIGRIRSVNGSKVHVVGT
                     YVRPLGLIDRIESGRAVGRPSEVLSNPDPNNCIFMLIDRVEAGRFDEVVDQSITRLIW
                     INESRYLVLDGWIKENLPDIFAAALSAGTEESRAHARQTLIEKMNSLYERELKEHVYA
                     VARSAKIL"
     gene            2051..>2473
                     /locus_tag="VE308_01835"
     CDS             2051..>2473
                     /locus_tag="VE308_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZA99742.1"
                     /translation="MQRFDELDMRILSELTKDASISVPRLSKKLNVNASVLYSRIKRL
                     AKRSLIKNFTVIVNENMLGINVKATVGINRDPKLKDPIHAELMKIQEIRSLTEVTGRF
                     DMIVNISAHTLEELHNVVIARIGKIEGIQNTETFVEMQR"
BASE COUNT          681 a          553 c          488 g          751 t
ORIGIN      
        1 cttgtaattg tcagaggctt gcaccttctt cacaaagccc gtgttgtcgc ctttataagt
       61 agagcctcca taaattatca gccagtagct gtcgctttgc tctacaaacc taagctttat
      121 ttcatcatct ggccaccaat cgatcgtctt tttccataag cggtaggcag acaccttctc
      181 ctcaaagagt ggaatgacat tcatatagat gtcatggtag cggtagccca caccgataaa
      241 gtccccgaac caagagatag gcttgctgtt cactgggtcc ataagcttgg cacggctata
      301 ataaaagaag gtgtgaaata aatcatgttc ttttttgtag ggtattgctc aaaagcatag
      361 agtattgatt ctgctatatt cacgcacgca cgcacgcgtg cacacaaaat cattgttttt
      421 tattaatatg acttgttatc agcctacact gatggaccta gcatatgccc aaatattgct
      481 ctatattgcg aatcgacaat ccttttttat ttttacatta ctagatcata tataatactg
      541 cgagcctata ggggtcaaag cgataacgaa aaggttgagc caaagatgat tctcaaccta
      601 cttttcttca acgctgacaa tcaagttata gttcactgtt ccttttcgac cggctcgata
      661 tgacactcat ggcctctgtg ctctttatct agctgctgtg cctcctcgtt tgttatagga
      721 aacttttctt cactgacacc acgcttttcc aatagaacct tctcacaggt atcgcagatg
      781 agttgcatca caatgatgcc cattttgcta aagtcaatcc agacgatata tttagactat
      841 tctgctagcc tactatgttg cgcatcctgc caaatattcc ctttctgttc ttttcattat
      901 cctctactaa cttgaaacat tcagagcaaa gagcgcgtag gttgactggt ctgttgtcct
      961 tgagggagcc attaatgtgc tgaaactgtg gcggtgtact gggcttgaac ttgacgctgc
     1021 atttctggca ccggttgtgg gcacgctcaa gcaccgtctg tcgtatagca agtgttaggt
     1081 gctcatactt ttttttatca taactggggc tgcttggagg accgaaaaat gacatgtatg
     1141 aaatagatcc tagctcaaac ttatgtcttg cgatcacgag caattttttt atagagataa
     1201 caataatcac agtaagaata aaaaaagcaa ccacagcagc tacgcactag caatcttctt
     1261 gtttttcttc cattcttttc ttttcttctt gctgcagtca gctaatatac atagtagcag
     1321 cagatcagct aacgattttt cccaacgagg ggattactta cagtatcttg gcacttcttg
     1381 ccacagcata gacatgctct ttcagttctc gctcataaag tgaattcatt ttttcaatga
     1441 gcgtttggcg tgcatgagcc cttgattctt ccgtaccggc cgaaagggcc gctgcaaata
     1501 tgtctggcaa attctccttt atccagccat caagcacaag atacctgctt tcgtttatcc
     1561 atatcagccg ggtgatggat tggtcaacca cttcgtcgaa cctgccggct tcaactcggt
     1621 ctatcagcat aaagatgcag ttgtttgggt caggattgct aagtacttcg cttggacgcc
     1681 cgactgctct gccagattct atacgatcta tgaggccgag cggtcggaca tacgtgccaa
     1741 caacatggac tttgctgcca ttcaccgacc ggatgcggcc gatgattatt ttgttatatt
     1801 ttccctcttt ttgcgctaca aaaacatatt ggccagactt aattcctccc ctgctcctaa
     1861 acatccatta gaatcccggt ttccaaatta tataatgtta tctcgacccc ggatctttgt
     1921 atgtgttatg gtttgcagca tcataagttt ttcatgatct agtactctcc aaactaagga
     1981 attgtgtttt ggcggtataa tccgctagaa ttacatttat taacactgtc caataaccat
     2041 agacaagcat atgcagagat ttgacgagct tgatatgaga atattatctg aactgactaa
     2101 ggacgcaagt atttcagttc caaggttgag caaaaaactt aacgttaatg cttctgttct
     2161 ttacagcagg ataaagcgtc tagcaaaacg cagtttgatt aaaaatttca ctgtgattgt
     2221 caatgaaaac atgttgggga taaatgtcaa ggcaacagtt gggataaacc gggatcctaa
     2281 gttgaaagat cccattcatg cagaactgat gaaaattcaa gagattaggt cacttactga
     2341 agttaccggc aggtttgaca tgatagtgaa cataagcgcc catactctag aggaacttca
     2401 taacgtcgtc atcgctcgca taggcaagat agaaggcata caaaacactg aaacattcgt
     2461 tgagatgcag agg
//
LOCUS       DASQTF010000076         3427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_937189,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000076 DASQTF010000000
VERSION     DASQTF010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937189"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="VE308_01840"
     CDS             <1..1422
                     /locus_tag="VE308_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZA99743.1"
                     /translation="SNTGEVKVAQCGLRRIEGALLKDYRRDEILLAHPEMLDRCVGPE
                     TKVVGINVMDPLGMAPVTTTMSPEKLSYVAMKFKKMCASIIQLKKKYNFKVVVGGNGS
                     WELAKPDRMKIHGIDTVVIGEADELALDLFHDLEAGDAPELLHTFVRSIENIPMMQGP
                     TVNSLIEAMRGCGRGCDFCDVNKRSKKDFPLARLQKEAKINLDYGFDSIWLQSDEMLL
                     YGCDNKDFIPNADAITELWSGLKSTGARFVGTTHMTLSAVASSPDLIKKMSEINEMNV
                     AGGRWLATNLGVETVAPRMVKKHLGVKTKPFQPEEWGSVVREGCRILNQNNWFPALTL
                     IIGWPDETPDETQYTIDLIEDFRHMNMRGLVAPLLYQDFSEKNSMHFGNLNEAQFTLF
                     WKCWEHNLRVINDIIPIIIRNKTYGPAMKVFMAALIKSGTWAVMRYLRGLSKELFNGQ
                     VPEDIVERYSRKQSVTATMPSRL"
     gene            complement(1429..1758)
                     /locus_tag="VE308_01845"
     CDS             complement(1429..1758)
                     /locus_tag="VE308_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99744.1"
                     /translation="MMKFEFEEFATVEDVFLYLVSVAPYMKQVMPVSSYKGYVFSLVP
                     LTPLSGDVLMMIYTKGKLDPGLVEFDISTKKFRPVTSVERADKNYFVILTPKVATLAE
                     EAIKNLP"
     gene            complement(1813..2655)
                     /locus_tag="VE308_01850"
     CDS             complement(1813..2655)
                     /locus_tag="VE308_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZA99745.1"
                     /translation="MVVDNKAKLSYVQIIKEDLGVFHITPDNGPIPDFKAGQFVTLGF
                     HIPSEGKIVRRAYSIASPPEQKKRFELYIRWVRKPVPGRLTTELFNKKEGDEIFWVKP
                     TGIFTINEKLPEDRVDDRRMVLIGGGTGLAPFLSYSLHLKAIGSKREIVVLHGASYVD
                     ELGYRDLLVSLEDESIDKGRDKWNFRYRSSISRPQEWFNRSWSGQKGRVESFLRPKPG
                     KELSPLEELVGEKVAPENTSFYICGWQGTIDGALDYLIPKGFVTERNKRKDGGYDIKF
                     ESYG"
     gene            2782..3069
                     /locus_tag="VE308_01855"
     CDS             2782..3069
                     /locus_tag="VE308_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99746.1"
                     /translation="MVDWLKGKDFGGSNEIDQLTKNINEQSDDQRKIISQFNKAMNEF
                     ATERSLETCLDALNLSMQLANIRGKLAGSYEYYARLLEREITRLSRTKPPS"
BASE COUNT          951 a          766 c          795 g          915 t
ORIGIN      
        1 tcgaacacag gagaggtcaa ggttgcacag tgtggcttga gaaggattga aggggcgctt
       61 ttgaaagatt acaggcgcga tgagatcctt cttgcacacc ctgagatgct tgacaggtgc
      121 gttggcccag aaacaaaggt cgtcggcatc aatgtcatgg atccacttgg catggcgcca
      181 gtgactacaa caatgtctcc agaaaaactc tcatacgtcg ccatgaagtt taaaaagatg
      241 tgcgcctcca ttattcagct aaagaagaaa tacaatttca aggtggtcgt tggaggtaac
      301 ggctcttggg agcttgccaa gcctgatagg atgaagattc atggtattga tacagtagtc
      361 attggcgagg cagacgagct ggctcttgat cttttccatg acttggaagc tggtgacgcg
      421 ccagaactct tgcatacctt tgtccgcagc atcgaaaata tccctatgat gcagggaccg
      481 acggtcaact ccctcatcga ggctatgcgt ggctgcggca ggggttgcga cttttgtgac
      541 gtcaacaagc gctcaaagaa ggactttccg cttgcgagat tacagaagga ggccaagatt
      601 aacctcgatt atggtttcga ctcaatctgg ctacaatctg acgaaatgct gctttatggt
      661 tgcgataaca aagactttat tccaaacgca gatgctatca ctgagctatg gagcggccta
      721 aaatctacag gagcacgctt tgttggaacg actcacatga cgctttctgc ggttgcatct
      781 tcgcccgacc ttataaaaaa gatgtccgag atcaatgaga tgaatgtcgc cggcggcaga
      841 tggcttgcaa ctaaccttgg ggtcgagacg gttgctccaa ggatggtcaa gaagcacctg
      901 ggtgtcaaga caaagccatt tcagcccgaa gagtggggtt cagtggtaag agaaggctgc
      961 aggatactta atcagaataa ttggttccct gcgcttacac taatcatagg atggcccgat
     1021 gagacaccag acgagacaca atacacgatc gacctcattg aggactttag acatatgaac
     1081 atgcgtggct tggttgcgcc gttactttat caagactttt ctgaaaaaaa ctcgatgcat
     1141 tttggcaacc tcaatgaggc acagttcacc ctattctgga agtgttggga gcataacctg
     1201 cgtgtaataa acgacattat accgatcatc atcaggaaca agacatacgg tccagcaatg
     1261 aaggttttca tggcagcgct catcaagtca ggcacttggg cagtgatgag atatctgcgc
     1321 gggctctcaa aggaattgtt caatggacaa gtgccagaag acatagtcga gcgatactcg
     1381 cgcaaacagt ctgtgacggc tacgatgccg tcacggctct agcatttttt atggtagatt
     1441 cttgatcgct tcctctgcaa gtgttgcaac tttgggcgtc aagattacaa agtaattttt
     1501 gtctgcacgc tcgactgacg tcacaggccg gaatttcttt gtcgaaatgt caaattcaac
     1561 aaggccgggg tccagttttc ctttagtata tatcatcatc aggacatctc ctgaaagcgg
     1621 agtcaatggc acaagagaaa agacgtatcc cttgtacgaa ctaacgggca tgacctgctt
     1681 catgtatggt gcaacagaaa caaggtaaag aaaaacatcc tctactgtag caaattcttc
     1741 aaattcaaac ttcatcattt taatcgcatt ttaccaagat ataaggcttt aaaaaatgaa
     1801 aaaagagagt gattagccgt atgattcgaa cttgatgtcg tagcctccat cctttctctt
     1861 attgcgctcg gtgacaaagc cttttgggat caaataatcc aacgcgccgt ctattgttcc
     1921 ctgccagccg cagatgtaga atgatgtgtt ttcaggggcc accttttcac ctaccagctc
     1981 ttcaagggga gagagttcct tccctggttt tggtctgagg aatgactcaa ctctgccctt
     2041 ctgtccgctc catgacctgt tgaaccactc ctgcggcctg ctgatgcttg agcggtaccg
     2101 gaagttccac ttgtcacgac ctttatcaat actctcatct tcaagagaga ccaagagatc
     2161 cctgtagcct agctcatcaa cataacttgc accgtggagc acgactattt cgcgcttgct
     2221 tccaattgcc ttgaggtgaa gcgagtatga taggaaagga gcaaggcctg tccctccgcc
     2281 aatcaaaacc atccttcgat catctactct gtcttcagga agcttttcgt ttattgtgaa
     2341 aatgccagta ggcttgaccc aaaatatttc gtcgccttcc tttttgttga aaagctcagt
     2401 tgtgagcctg ccaggcaccg gcttacgtac ccagcgtatg tatagctcaa agcgcttctt
     2461 ttgttcagga ggtgatgcga tagagtaagc tctgcgtact attttgcctt cgcttgggat
     2521 atggaagcca agcgtgacaa actgacccgc cttaaagtca ggaattgggc cattatccgg
     2581 agtgatgtgg aagacgccaa ggtcctcctt gattatttgg acgtatgata gcttggcttt
     2641 gttgtcaacc accatatact tctgaagttc agaaaacggc aaataaatac atttttaatt
     2701 ttcaggccta ccagtaactg ctcaaatgga caaaaatagg caagttttat ttgattctgg
     2761 ctccaatcta gtgttgtata tatggtagac tggctcaaag gtaaggattt tggaggctct
     2821 aacgagattg atcagcttac caagaacata aatgagcaga gcgacgatca gaggaagatc
     2881 atttcccagt tcaacaaggc tatgaacgaa tttgcaactg agcggtcgct tgaaacttgc
     2941 cttgatgccc ttaacttgtc aatgcagctt gcaaacatca gagggaagct ggcagggtcg
     3001 tacgaatact atgctaggct gttagaacgc gaaataacaa ggctgagtag aacaaaacca
     3061 ccatcctagt ctgatacaag gatcggcgtg acaatttctc ataagacagc gcttaaagcc
     3121 aaccttccat tttgagtgag gcagcaatag tgggaatatt ccttagttcc atcaaatctt
     3181 tgagaagtat gctcagctct cttctatatt ttatagtgat gtatatgaca gcagtatctt
     3241 agcaacagac ataagtttgt attttaatag ttgtccaaaa cgtagtaaga cagtagttca
     3301 tgcagccaag cagactaata tcacatatct tctattacgg tgtggaataa attgcagtcc
     3361 atcacctctg ccacattata tttcgtcgtt ttaggtttct gtgtcactag agattaaaaa
     3421 tcctctc
//
LOCUS       DASQTF010000077         3379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1126078,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000077 DASQTF010000000
VERSION     DASQTF010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126078"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1169)
                     /locus_tag="VE308_01860"
     CDS             complement(108..1169)
                     /locus_tag="VE308_01860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZA99747.1"
                     /translation="MVSKMQVGIDSFAAAFDDASLAVSPFERLRDLVEQIERADQVGL
                     DVFGVGEHHRREFLDSAPTVILGAAAARTQRIRLTSAVTVLSAADPVRVFQEFATLDL
                     LSQGRAEMVVGRGSFIEAFPLFGLRLDDYDSLFAEKLDLLLKIRDNEQVHWSGEYRPT
                     LNGQGVYPRPLQKPLPIWVGVGGTPQSFVRAGALGLPLMVAIIGGETRHFRSLIDLYR
                     EAGRRAGHSPDQLKVGIHSLGYVAETTQQAADDFFPGYARAFTDIGKERGWRPVTRAG
                     FDAQRGPQGALIIGDPDEVVKKIVRHSKALGGISRITFQMNAASLPHVKLMRAIELIG
                     TRVAPALREELVRNDGRDG"
     gene            complement(1804..2127)
                     /locus_tag="VE308_01865"
     CDS             complement(1804..2127)
                     /locus_tag="VE308_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZA99748.1"
                     /translation="MSKPQFLYLTTRGWKTGKQHRIEIWFEKHNERYYIISERLDSAH
                     WVQNIKRNPIVSFSINDNVFKGNARLVDQEKESALAAEVLKLMDAKYKWSQGLIVELT
                     PTNST"
     gene            complement(2173..2661)
                     /locus_tag="VE308_01870"
     CDS             complement(2173..2661)
                     /locus_tag="VE308_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99749.1"
                     /translation="MQAVATSANTIRDVDFNSPFDNTYDLGNPFLVEYDNTTSLQPPR
                     SANYDFEAVFSGYGIINGTRYTDTGISSYDIRGNGSSVVYQNGTIAMTTEAGEKAMMI
                     FESLGQRNTTSGIVLDHGVIFLITNSTTGELASLNNTVYVYKDKVDERRGNLTTIAWQ
                     WK"
BASE COUNT          930 a          774 c          711 g          964 t
ORIGIN      
        1 ttggacattt tgcttttgag gctcatatga aagaatggtt ccttcaaacc atcctacgat
       61 attgatatac ttgctgacac gcgacaaggg cggtcctgcc taacatctca accatctctc
      121 ccatcatttc taacgagttc ttcgcgaagt gcaggcgcaa cgcgggtgcc tatcaattcg
      181 atagctcgca taagcttcac gtgaggtaat gaggctgcgt tcatctgaaa agtgatccgg
      241 gagatgccac cgagtgcttt gctgtgccga actatctttt taaccacctc atccggatca
      301 ccgatgatca gtgcgccttg tggccctcgc tgagcgtcaa agcccgcacg cgtcactgga
      361 cgccagccgc gctcctttcc aatatctgtg aaggcacgcg cgtagcctgg aaagaagtca
      421 tcggctgcct gttgcgttgt ctcagccaca tagcccagcg aatgtatgcc taccttcaat
      481 tgatcgggtg aatgtccagc tcgcctccca gcctcccggt agagatcgat gagtgaccgg
      541 aagtggcgcg tttcgccgcc tatgattgcg accatcaagg gcagtccaag ggctcctgcg
      601 cgaacgaatg attgtggagt tccaccaacg cccacccata tcggcaaagg tttctgcaag
      661 ggcctcgggt agactccctg tccattcaag gtaggccgat attcgccgga ccaatggacc
      721 tgctcattgt cacgaatctt cagaagcaga tcgagtttct ctgcgaaaag cgagtcgtag
      781 tcgtccagtc gcaggccgaa caggggaaat gcctcgatga acgacccacg gcccacaacc
      841 atctccgccc gaccctgtga cagaagatca agggttgcga attcttgaaa aactcgaacg
      901 gggtcggcgg cgctcagtac cgttacggca ctggtgagac gtatacgttg cgtacgcgcg
      961 gctgcagctc ctaggatgac agtaggggcc gaatcgagga actcccgccg gtgatgttcg
     1021 ccgactccaa atacatccaa gccgacctga tcggcgcgtt cgatttgttc aactagatcc
     1081 cgcaaacgct cgaatgggct gactgcgagg ctggcgtcgt caaatgccgc tgcgaagcta
     1141 tcaattccaa cttgcatctt tgaaaccatt tcttcttctc ttgcctcgtt cttctacatt
     1201 atttgtactt ttgagacaga acgcgctttg tgctggttag aaggcgaatt cagcctgtct
     1261 actaacaaat ttttcgccat aatgtcataa tcaacctgat tctatttaag gacggtagca
     1321 atacattact tagtaagtca gttgcgccaa atatagtgcg cctgagttga cctacaatta
     1381 gcagacgcta ggctgtatca tagcgggccc atcgggattc ggatagaaat tctttgaatc
     1441 caaagacctg atttcagctt attgttagtg tttatacatc cagatagatg tgaaagacct
     1501 tcaaaattta gacataaacg ttccgtaaca ggacacagaa tttattgtta tgggaatgac
     1561 tgcaataggc aagagggcat tataagaaac cctgtcagca gagctccggc taaaagagtt
     1621 gctgccaagt aataggaagc ctctggaaga ggttattatg gaagggacat tcaggacaga
     1681 gcaataagca aaaagaattt cggtgatgcc ctgatttttt gatggacgta atagagctat
     1741 aaatgtagtt aatatagcta cgtcaacttc aatcaacaat gtattgagga agtcttgaaa
     1801 tgatcaagtt gaatttgttg gcgtaagttc tacgattaat ccctgactcc atttgtattt
     1861 tgcatccatt aatttcaaaa cctctgcagc caatgcagac tctttctctt gatcaactag
     1921 tcgtgcgttg cctttgaata cattatcatt tatgctaaat gaaactattg gattgcgctt
     1981 aatattctga acccagtgcg cgctgtcaag tcgctccgat attatataat atctttcatt
     2041 atgcttttca aaccagatct ctattctatg ttgtttgcct gtcttccatc cccgagtggt
     2101 caaatatagg aactgtggtt tactcaatta tgattttgta acaccttgtt attcactttc
     2161 aattacatca aatcatttcc attgccatgc tatggtagta agatttcctc ttctttcatc
     2221 taccttatct ttgtatacat atacagtatt gtttagagac gctagctctc ctgtagtaga
     2281 attagtgatc aaaaagataa caccatgatc gagtactatg ccacttgtag tatttctctg
     2341 tcccaaagac tcaaaaatca tcatagcttt ttctcctgct tctgttgtca tggctattgt
     2401 tccattttgg tagactacag aagaaccatt accgcgaatg tcatagcttg atatcccagt
     2461 atctgtgtat cttgtgccgt tgattatgcc gtatccagag aaaacagctt caaaatcata
     2521 gttagcactc cttggtggtt gcaggctcgt agtattgtca tattctacca agaaggggtt
     2581 tccaagatcg tacgtattat caaaaggtga attaaagtca acatctctga ttgtatttgc
     2641 agaagtagca accgcttgca ttggatatag cacaatcgta taaagaacaa aaaggctcgc
     2701 tcctaacagt atggttctgt tacccatgtc ctatatgacc atcagttgat cagcatcatc
     2761 atttaaacga agttctaaga aatgtagcga atcttaccta tcaattgatt atattatttg
     2821 actacatagt gccacatgcg gaatttgcga ttgaaccgtc atcgcctatt gtcacttgtg
     2881 ctattaagta ttgtgtccat ttcttgaaga ttttgcagca ggcccaatga ttcgaaagaa
     2941 aaaccttcaa aatatagact caatacaaat agaagccgaa atttccagtc cctattcacc
     3001 ttgcacttac agaaactatc cataatttct actcgcggca actattttta gatcttccct
     3061 actgaagaat aagaagacat atggttgcca ttttttctca aaagtctggt tcacttataa
     3121 ggtgtcatca ccaagtcttt aatattgtgg aagacaatct gtgaccgatt gtataaatgc
     3181 cctcaaggct cttttatatt aacaaaggac tgggactcgt caaccagacg atcgactcaa
     3241 gacagaatgg aaagtgcaga gattgtggga agaactttac ccctgacgaa caaattgtaa
     3301 gtaatggcaa aaggaagaaa tattatcatg tagagtgtgc acagaggctg aatatcctta
     3361 tactccttcc taaaggatt
//
LOCUS       DASQTF010000078         7490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_474213,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000078 DASQTF010000000
VERSION     DASQTF010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474213"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VE308_01875"
     CDS             complement(<1..334)
                     /locus_tag="VE308_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZA99750.1"
                     /translation="MKAVFIKGHNAVSVEDVKVPGLASPGDVLVKMRVCGLCGSDLEK
                     IYGEYSMASGRLGHEPSGEVIAIGKSVKGFARGDRVFIHHHVACYSCHYCLHGDYTMC
                     PAYQTSNIS"
     gene            434..838
                     /locus_tag="VE308_01880"
     CDS             434..838
                     /locus_tag="VE308_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZA99751.1"
                     /translation="MLLPSEIEAKSLIPAVRAILAKKLIRQYSLKEEDVAKDLGITQA
                     AVSNYVRGTRGDIELISKLESVREVMRMIDDIAKDLSTNKAYTPSTLAKFVGLCNYMR
                     YTLIICDVHHSIESNIDEQICEQCRGTLVREH"
     gene            910..1158
                     /locus_tag="VE308_01885"
     CDS             910..1158
                     /locus_tag="VE308_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99752.1"
                     /translation="MTRGGGKQWINGETITGIALVFLALLFIWAATMNAVWARILLAD
                     YVILAIGIGFIVLGIITMRRTNRPHSEEEEEEGHTHRY"
     gene            1205..2032
                     /locus_tag="VE308_01890"
     CDS             1205..2032
                     /locus_tag="VE308_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZA99753.1"
                     /translation="MPDASDDVLTTFANTEKTFFELASEQRLAIVFRLNNKSAKISQL
                     AKDLDITIQEVHRNVSRLQDAGLIEKDPEGIFSLTTFGNTIIKQIPTFNYLSKYKEYF
                     SEHVLGELPIKFIMRLGALDKCEFVKGIVAILERWKDIHRQADQYIYEIVPQVPIDLI
                     EPAVTRIKEKAVKYSYVFPKNVIIPKGRKELLKKLGHNELLSKGAVERRMVESVQVAV
                     ILNEKQASVLFPTQKGETDMNMMFFSPDPIFHEWCLDYFRYRWYGSDIFDESRLKEI"
     gene            complement(2045..2503)
                     /locus_tag="VE308_01895"
     CDS             complement(2045..2503)
                     /locus_tag="VE308_01895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF002207.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HZA99754.1"
                     /translation="MVVKRSSKRHAAPKKPATAAKTTKKTSASSRKVGTNEKQEEEQG
                     GTTKKSSRSADEVVIYAEYEYELKEIDAQDNKVLIGPAWLTRFEKARITGARSLQLSL
                     GAPPLVKVPEHATSSIAVAVEEIEHKALPISIRRILPSGTYQDISIEWLK"
     gene            complement(3203..3529)
                     /locus_tag="VE308_01900"
     CDS             complement(3203..3529)
                     /locus_tag="VE308_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99755.1"
                     /translation="MGGNKKKAAGASDKSAQTGPGQAETKSAAEKKTSSKPQQKQKLA
                     VTIEEPQGMKAIQGMKAITTQGVARNIGVKISVANAFIKSLEAKGIVKAEGGYSGHRV
                     YHLIKR"
     gene            3955..5751
                     /locus_tag="VE308_01905"
     CDS             3955..5751
                     /locus_tag="VE308_01905"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZA99756.1"
                     /translation="MDFNSLLVTFEQMEQTSSRIALTGFLVTLLKKTPVDIIDKVTYL
                     IQGKLYPDYEGIEMGVAEKMVLRAIASSSGKDVSEIGRIYQKTGDLGEATLEVMKSKG
                     QSTLFSEPMTVERVYSTFDKIARTTGAGSQENKLRLISSLLNDAKAAEGCYIIKFVMG
                     QLRLGIADYTVLDALAIAFTGDKANRQMLENAYNVSSDLGTVARLLATKGLEAVSSIK
                     ITLFKPIRPMLAERIRTSEEALERMGSRAAVEYKLDGERVQVHKGKDRVELFSRRLEK
                     ITSHYPDVANALKSIKSEVILEGEVVAINIETEEYLPFQELMHRRRKYGVEEAMESYP
                     VIMNFFDVLYFDGKSKTEMSYMERRKLLKKIIDRIHNNKLSLIKQTIATEPYEIDRFM
                     AAAIENGCEGVMIKHLSSTYRAGAREYAWVKLKREYTSDFADTLDLVIVGALHGRGRR
                     AGKYGALLLAAYDHNTDMFRSVTKVGTGFTDVHIEQFYKELEKHIVSQRHARVDTGME
                     MDIWFEPKIVIEVIASEITLSPSHTAAKNTIRPGYGLALRFPKFTGKIRDDKKPEDAT
                     TIDEIVAAYKRQLKVVGHSGAKVQVEEEDKEV"
     gene            complement(5860..6576)
                     /locus_tag="VE308_01910"
     CDS             complement(5860..6576)
                     /locus_tag="VE308_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZA99757.1"
                     /translation="MEFVNLEKPDVSKPIMIAAMQDMGNVGSIAIDFINKSLNSRLFR
                     YVLTPYPNYVVDKGGHIDFHQERWEYRYIKSIIVFGGGIGQPQTNYELYELCQDVINI
                     AKLYSVQLIYTLGAFHTDRNYGKDPVTLVTTTSQELTDQIVKVGHNTTPGSSLITGFN
                     GLILGYAKRNNIQGIGLFAEIDDPQMLQYRSAKSLLVSLEKLTYQRFKGLEEMDEMAD
                     AIERELDRMKRMGDDSLYRP"
     gene            6666..6827
                     /locus_tag="VE308_01915"
     CDS             6666..6827
                     /locus_tag="VE308_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99758.1"
                     /translation="MSGGPLISDDYKIAIEGWKARKDQLLRQQESVTRINYRAEKVNI
                     LSLLKQKNN"
     gene            6824..7468
                     /locus_tag="VE308_01920"
     CDS             6824..7468
                     /locus_tag="VE308_01920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZA99759.1"
                     /translation="MKVKISYVYIIAGVDSKVLRVISRLNRQSNRERSGRVKVAPDQE
                     MLAITAETGIFFSVLLKGIKARRILEVGTSAGFSTLWFADAMGKNHSTRVITIEMNPQ
                     KAEWALKNFKEAGVDKMIEIKQGVALDLLHKLKGKFDFVFLDADKENIIRYFDLVLPM
                     VRIGGIIAADNMLYPDHFRPAMKKYARHVNSKPNTQSVTVPIGMGEEITIKLCN"
BASE COUNT         2161 a         1623 c         1677 g         2029 t
ORIGIN      
        1 ggctgatgtt gctcgtctgg tacgcagggc acatagtata atcaccatga aggcagtaat
       61 gacaagaata gcacgcgaca tgatgatgga taaagacacg gtcgccacgc gcaaagcctt
      121 tgacagattt gccaattgct ataacctcgc cggaaggctc gtgaccaagt ctgcctgaag
      181 ccatgctata ttcgccatat atcttttcta gatccgaccc acacagcccg cagacacgca
      241 ttttgacaag aacatcacct gggctggcca ggcctggtac cttgacatct tcgacagaaa
      301 cagcgttgtg tcctttaata aaaactgcct tcatatttct tatcctcaat ttttgccaaa
      361 tataaagaat agaatccatt aatccattaa gaacctttaa atagtaaaaa ataaatttca
      421 tttgcatccc taaatgctgc tcccttcaga aatagaagca aaatcactca tcccagctgt
      481 aagggctatt cttgcaaaga agcttatcag acaatattcc ctcaaggaag aagacgttgc
      541 caaagatctt ggtattacac aagccgcagt tagcaactac gtgaggggaa caagaggaga
      601 tatagagctc atttcaaagc ttgaatcagt tagagaagtc atgcgcatga tagacgacat
      661 tgccaaggat ctctctacaa acaaagctta cactccaagc acacttgcca agttcgtggg
      721 tttgtgcaac tatatgcggt atacactcat tatatgtgac gttcaccaca gcattgagtc
      781 aaacattgat gagcagatct gcgaacagtg cagaggtact ttggttcgtg aacattagcc
      841 ttcataattt ttctaactaa ttaataattg aagaatttaa taatgttgcc ggagtgctat
      901 attggatata tgacgagagg cggaggaaaa caatggatca acggcgaaac catcacaggc
      961 atcgcgctag tgtttttggc acttttgttc atctgggcag cgacaatgaa cgctgtatgg
     1021 gctaggatac ttttggctga ctacgtcatc ttagcaattg gcataggctt cattgtactg
     1081 ggcataataa ccatgcgcag gacaaatcgt ccgcattcag aagaagaaga agaagaagga
     1141 catacccata ggtactagag tgtatatgta atgtttaaaa cattgatcag tttcatgcaa
     1201 taccatgcct gatgcctccg acgatgtcct gacaacattt gccaatactg agaagacatt
     1261 ctttgagctt gcaagtgagc aaagactagc aatagtattc cgcctcaata ataaaagcgc
     1321 caagatatcg caacttgcca aggatctcga catcaccata caggaggtcc atcgcaatgt
     1381 cagtcgcctg caggacgcgg gcctaattga gaaagaccct gagggcatat tttcacttac
     1441 cacttttggc aatactataa tcaagcagat cccaacgttc aactatctct caaagtacaa
     1501 agaatatttt tccgagcatg tattgggcga gctcccaatc aaatttatca tgagactggg
     1561 ggcgcttgac aaatgcgagt tcgttaaggg cattgttgct atacttgagc gttggaagga
     1621 cattcatcgg caggctgacc aatacatcta tgaaattgtg ccgcaggtgc caatagacct
     1681 aatcgagcca gcggtgacta ggataaagga aaaagccgta aaatatagct acgtctttcc
     1741 aaagaatgtc atcatcccaa aaggcagaaa ggaacttttg aaaaagctcg gtcataatga
     1801 actcttgagc aaaggggcgg tagaaagaag aatggtggaa agtgtgcagg ttgcagtaat
     1861 actgaacgaa aaacaggctt cagtactttt cccaacacaa aagggggaga ctgacatgaa
     1921 catgatgttc tttagccccg accccatttt ccacgagtgg tgccttgact actttcgtta
     1981 ccgctggtac ggctccgaca tctttgacga aagcaggctg aaagaaattt aaattctgat
     2041 ctggctactt caaccactct atagatatgt cttggtatgt accgcttggc agtatgcgtc
     2101 ttattgaaat cgggagcgct ttgtgctcta tttcctcaac tgcaactgca atcgagcttg
     2161 tagcatgttc aggcaccttg acaagcggag gtgcgccaag tgaaagctga agtgaccttg
     2221 cacctgttat ccttgctttc tcaaaccttg taagccaggc aggaccaatc agcaccttgt
     2281 tgtcctgagc atcgatctct ttcaactcat attcgtattc tgcataaatg actacctcgt
     2341 ctgcgctgcg cgaagatttc ttggtggtgc cgccttgttc ttcttcctgc ttttcatttg
     2401 tgccaacctt tctacttgaa gcactagtct tcttggtcgt tttggcggca gtagcaggtt
     2461 ttttgggtgc agcatgtctt ttggaagaac gtttgactac caagaataat ccaaaggata
     2521 atggatatct aattaaatgt tttgcctaat ttttcaaaac ttacactgga gctatattat
     2581 ctgcctaaga aaataggtat ctgatattta tattttcact agcgtttttg atgctgtcag
     2641 gattctttca ctatatatct gccatttgct ttggtattac cctttatggc tactttggcc
     2701 acttccccac ctctgtattt tttggctctt ttgtactgac cttgcaattg atagcattcc
     2761 tcatctattt taataacatt cgaaacgttt tcatacgttc tatgcctatt tgcagcaagg
     2821 atgcagccag gaagcgctgg gtcagtatct agattaaaga ttgcaatcga aatacaagag
     2881 aaaggctctg catcggccga actagtgcgt cactttgctc cattgactat tggaaccgta
     2941 ctgaaaagcc ttccgctgca agacagagtg cataggtatg tagacaaatt cgtatatatc
     3001 gaaactgggc ttatgatcgg accagaaaaa catagaacac aatttcactg cggtgatatc
     3061 gcttacctta cttcaaatag ctccatttgt gtcttcgtgc aagatgctaa agtccagccg
     3121 atgaattcgc tgggtatagt cacctctaac cttgaggtta ttgagtcgag tcggcccggg
     3181 gacgtgatga tagttaaaga agctagcgtt tgatcaggtg atagacccta tggccgctgt
     3241 agccgccttc agccttaacg ataccctttg cttccagtga cttgatgaaa gcgttggcga
     3301 ctgaaatctt gacgccgatg ttccttgcta cgccttgtgt tgttattgcc ttcatgcctt
     3361 ggattgcctt catgccttgg ggttcctcaa ttgtcacagc aagcttttgc ttctgctgag
     3421 gcttggagct tgttttcttc tctgcagccg acttggtctc ggcttgtcct ggccctgttt
     3481 gggcactctt gtccgaagca ccagctgcct ttttcttgtt gcctcccatt attttacata
     3541 cctgctcgcc accctttaat aaggttagct agccttgcag taaacttcat tattttggct
     3601 tcttcagctt tcgctacatt ctctcaatag gtggcagttg cctcatcaat tgctatacaa
     3661 ctgttctgat acctcttgtc ttacagaaga ttttagtcct tggtgtagta actgctctgc
     3721 tacttgacaa cctttaattc ttcttccgca aacttgatcc gtcgctgtcg ctgccctacc
     3781 tgcgtctcgg tcttgtagaa ctgggttaag atctccagcc aatactttgt atgctttatc
     3841 tgctccctac aggatcaatg ctgattatct aggctcttaa tgctatgcca tgcttctcgc
     3901 cttaaaacat atcatagatt aatattcact caaagcgtac agagttgaat cgccttggat
     3961 ttcaattcct tattagtgac atttgagcag atggaacaaa cctcaagcag aattgcgctt
     4021 accggattcc ttgttacgct tctcaaaaag acacccgtcg acattattga caaggtgact
     4081 tatctaattc agggcaaact ttacccagac tatgagggaa tagagatggg agtcgcagaa
     4141 aaaatggtcc tcagagcaat tgcaagctcg tctggcaaag acgtatcgga aataggacgg
     4201 atataccaaa agacaggcga ccttggagag gcgacactag aagttatgaa gtcaaagggc
     4261 caatcaacac tattttcaga gcccatgaca gtcgagcgcg tatattctac ttttgataaa
     4321 attgcacgga caactggcgc tggttcacag gagaacaagc tgcgtctaat aagcagcttg
     4381 ctgaatgatg caaaggctgc agaggggtgc tatataatta agttcgtcat gggacagctg
     4441 cggcttggca ttgctgatta tacagtgctt gatgcccttg caatcgcatt cactggtgat
     4501 aaagcaaaca ggcagatgct tgaaaatgcg tataatgtct caagcgacct tggcacagta
     4561 gcaaggttgc ttgcaactaa ggggctcgaa gcagtcagct cgatcaagat aacactgttc
     4621 aagcctatca ggccgatgct tgcagaacgt attaggacct cagaagaagc acttgagcgc
     4681 atgggaagca gggcagcagt agaatacaaa ttggatggtg agcgcgtaca agtgcacaaa
     4741 ggcaaggaca gagtagaatt gttttcaagg aggctcgaaa agattacgag ccactatcca
     4801 gatgttgcca acgcattaaa gtccataaaa tcagaagtga tattggaggg cgaagtagta
     4861 gccataaata tagagacaga ggaatatctt ccctttcagg aactgatgca tcgacgacgg
     4921 aaatatggcg tagaggaggc aatggaaagc tatcctgtca tcatgaattt ttttgatgta
     4981 ctctacttcg atggcaagag caagacagaa atgtcataca tggaaaggag aaagctgcta
     5041 aagaaaataa ttgataggat ccacaataac aaattaagct taatcaagca gactattgca
     5101 actgagccat atgagatcga taggttcatg gcggctgcaa ttgagaatgg ctgcgagggc
     5161 gtcatgataa agcatctttc aagcacatac cgcgcaggtg caagagaata tgcatgggtc
     5221 aagctaaaac gtgaatatac aagcgatttt gcggatacac tggatcttgt catcgttggc
     5281 gcactgcatg gccgcggaag gcgcgcaggc aaatatggag cgttactgtt agcggcttat
     5341 gatcataaca ctgacatgtt ccgcagtgtg accaaggtag ggactggctt taccgacgtg
     5401 cacattgagc aattctacaa agagctcgag aagcatatag taagccagcg gcacgcaagg
     5461 gtcgataccg gaatggaaat ggacatctgg tttgagccta aaatcgttat tgaagtcatt
     5521 gcctcagaga tcaccctgag tccatcgcat acagctgcca agaacacaat aaggccgggc
     5581 tatggtcttg ctctacgctt tccaaaattc acaggcaaga taagggacga caaaaagcca
     5641 gaagatgcca cgactattga cgagattgtg gctgcataca agagacaatt gaaggtagtc
     5701 ggccactcag gtgcaaaggt gcaggtagag gaggaagata aagaagtttg acgcactgca
     5761 ctctagtaag aagtgagaca ttgcatatcg agtataatat tgttccagtg atatatagat
     5821 gctagatagg tttgactaag cccctccaag atcaactcat tagggacgat ataaagagtc
     5881 gtcgcccatc cttttcatcc tgtcgagctc tcgttcaatt gcgtcagcca tttcgtccat
     5941 ctcctctagg cccttgaacc tctggtatgt cagcttttca agcgaaacta gcaggctctt
     6001 tgctgatcta tattgcagca tctgaggatc atcgatctct gcaaacaacc cgatgccctg
     6061 aatgttgttt cttttagcat agcccagtat caggccgttg aaacctgtta tcagcgacga
     6121 tccaggagtg gtgttgtgac ctactttcac tatttggtct gttagctctt gtgaggtcgt
     6181 tgtgacaagt gtcactgggt ccttaccata gtttcggtct gtgtggaatg cgcccagggt
     6241 ataaattagt tgcacggagt acagcttggc aatattaatg acatcttgac acaattcata
     6301 aagttcatag ttggtctggg gctgcccaat tccgccccca aaaacgatga tgctcttgat
     6361 gtagcggtat tcccatcttt cctgatgaaa gtcaatatgg cctcctttat caacaacata
     6421 atttggataa ggcgttaaga cataccgaaa caatcggcta ttgaggctct tgttaataaa
     6481 atctatggct atactaccaa cgtttcccat gtcttgcatg gcagcaatca ttataggctt
     6541 acttacatca ggcttctcaa ggttgacaaa ttccactgca ctttctcttg acagcagata
     6601 ataaaaaagt tagtagtttg attcctgcaa cgattaaaag taggccttcg cgttgttgat
     6661 ttaccttgtc agggggtcct ttaatatccg acgactataa gattgcgatc gaaggctgga
     6721 aggcacgcaa agaccaactg ctcagacagc aagaatcggt aacgaggata aactacaggg
     6781 ctgaaaaagt gaacatttta tcacttttga agcagaagaa taattgaaag taaaaatatc
     6841 ctatgtatac attattgcag gcgtggacag caaggttcta cgcgtaatat ctcggcttaa
     6901 taggcagtca aatcgagaaa gaagtggcag agtaaaagtc gcgcctgatc aagagatgct
     6961 tgctatcaca gccgaaactg gcatattctt ttcagtactt ctcaagggaa tcaaggcaag
     7021 acgcatatta gaagtgggta catcggcggg gttttccaca ctgtggttcg ccgacgcaat
     7081 gggtaagaac cacagcacaa gggtaataac gatagaaatg aacccacaaa aagcagagtg
     7141 ggctttgaaa aatttcaaag aggctggtgt tgataagatg atagagataa aacaaggcgt
     7201 cgcccttgac ctgctgcaca agcttaaagg caagtttgat tttgtcttcc ttgatgcaga
     7261 taaagagaac atcatacgat attttgacct tgtcctacca atggtaagaa ttggagggat
     7321 aattgctgca gataatatgc tttatcctga ccatttccga cccgccatga aaaagtatgc
     7381 gcgccatgtg aacagtaagc ccaataccca gtcagtcact gtgccaatag gaatgggaga
     7441 agagattacg atcaaattgt gtaactaatt ctgaacatct ccctttcaaa
//
LOCUS       DASQTF010000079         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_568945,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000079 DASQTF010000000
VERSION     DASQTF010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568945"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..485)
                     /locus_tag="VE308_01925"
     CDS             complement(150..485)
                     /locus_tag="VE308_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZA99760.1"
                     /translation="MTYTMADKITAKDLADKKGDFVLIDVREADEISEDGGSIIEGAV
                     NIPLGQLIRTARQGGLDNLKGKTICTYCNGGYRGNIGADELNKKGFTSVTINGGYAAW
                     KEEEKKNKK"
     gene            1056..1574
                     /locus_tag="VE308_01930"
     CDS             1056..1574
                     /locus_tag="VE308_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99761.1"
                     /translation="MHRAHNKNNCKIAVAASLFTLAFFLVADSTGGSAVYGQVERTNS
                     FTDPTTNLRSPRAPVVVSGDNIYVAWWTNNTENGNEEVMFRASTDGGATFGDRINLSN
                     TTDADSWRVEIHGEADTVVVSWWETNQTSDTPVARISNDAGETFGPILRLAANGTIDS
                     TAEEVIATEEGG"
     gene            1812..2081
                     /locus_tag="VE308_01935"
     CDS             1812..2081
                     /locus_tag="VE308_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99762.1"
                     /translation="MTSGSTGEKIKEKVKGTIGAGEDSGQNIKEKVKEKIGMGGGGGS
                     EGRKEELGPGAATKLNEEVAKTSSDSPTKKVEGPGKALGKEEGTQ"
     gene            2352..>2900
                     /locus_tag="VE308_01940"
     CDS             2352..>2900
                     /locus_tag="VE308_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZA99763.1"
                     /translation="MLKVAVIGATGAVGQEFIIALNKHKWFELTQVAASERSAGKKYV
                     DAIRDPNSGILRWHNREEVPEYIKDMVVSKVDDIEPENFDLIFTALESDDAKIIEPKF
                     AKTTPVISTAAAFRYETDVPILIPGINDNHAELLNIQRKNRSWKGFIAPLPNCTTTGM
                     AITLKPILDRFGIDRVFMTSMQA"
BASE COUNT          883 a          572 c          649 g          796 t
ORIGIN      
        1 agaccaacca ttgcgccgac aaaggcattg actacaatga gaattacaaa ttgactaagg
       61 ttctgtcgta atccctgttt tattgtcacg tccttgtcct ttccgttgtc cttaccaata
      121 cctttcaata acaggtttag aggtagagct catttcttat tcttcttttc ctcctctttc
      181 caagctgcat agcctccatt aatagtcaca cttgtaaacc ctttcttgtt aagttcatca
      241 gcaccgatat tgcctctgta tcctccattg cagtaagtgc atatggtttt tcctttcagg
      301 ttgtctaatc caccctgcct tgctgttctt attagttgtc caagtggtat attcacagct
      361 ccttcaatta tgctaccacc atcttcagat atttcatctg cttctctcac atctattagg
      421 acaaaatcgc ctttcttatc tgcaaggtct tttgctgtta tcttgtcagc cattgtgtat
      481 gtcaagcatc gtgtatttgc aatttgaatt ctttgatggc acctttgtat gcacttcttg
      541 cgcatagtcg tgctagatag aacgcgccta gagacgcaga taagttaaga cggcttttga
      601 aagtgaaaga aagacaatag gaggaagcta tgcatataga agacacagaa aggctagtta
      661 cagaggttga aatgctcaaa gtcgtatttt tttattcata tgctcctgca agattatctt
      721 catgaatccg cattagtaac tatcataaac tgccagcatg ccaagggaaa ttggttggta
      781 ccactagcga actttagctc ttgccgcata gactacagca gcaatgtctc atacccaatt
      841 gcttcttctt tgttgtcttc aagaatatct tcgtatatgt cgctacagcc actatagcct
      901 caagctacag atggttggtt aaagcatggc tgacagcgaa aggggagggg gttttgcttg
      961 tcctcttaat caaacgtcga gcttgataac tactgtgtaa tatttcaaga actaattctt
     1021 ttattcttct tattagtgct tgacagtgag agattatgca tagagctcat aataagaaca
     1081 actgcaagat agcagtagca gcaagcctat tcacattggc atttttctta gttgctgata
     1141 gtactggtgg tagtgctgtt tatggtcagg tagaaaggac aaattcattc acagacccaa
     1201 ctaccaactt aagaagtcca agagcacctg ttgtagtgtc tggagataac atctacgtag
     1261 cttggtggac taacaacaca gaaaatggca atgaagaagt aatgtttaga gcttctacag
     1321 atggcggtgc tacttttggt gatagaataa acctaagcaa cacaactgat gctgactctt
     1381 ggagagtaga gatacatggc gaagcagaca ctgtagttgt tagctggtgg gaaactaatc
     1441 aaacaagtga taccccggtg gctagaataa gcaatgatgc aggagagaca tttggtccta
     1501 tcctaagact tgctgctaat ggtactattg acagtacagc agaagaagta atagcaacag
     1561 aagaaggagg atgaggagga gaagcggctg aacaacctaa tgatggcggt ggtatttatg
     1621 gtactttctg cctcttatct catcttctag gaagctgaca tggcggcagt ggtgacggtg
     1681 gttagttagg actggtttca catccttcat tatagttttt aataattcac taatctagaa
     1741 tcaggttata gaataaatct tcattatgat tctctagtta atattaatat tttgttagcg
     1801 gtacacttca catgactagc ggttcaacag gagaaaaaat caaggaaaaa gtaaagggga
     1861 cgattggagc tggagaagat agcggacaaa atatcaagga aaaagtgaag gaaaagattg
     1921 gaatgggcgg cggaggaggt agtgaaggta ggaaggagga gcttggaccg ggggctgcta
     1981 ctaaacttaa tgaagaggtt gcaaagacaa gctcagacag cccaacaaaa aaggtcgaag
     2041 gtcctggtaa ggctttagga aaagaagaag gtactcagta aagatacagg gtctatgaag
     2101 atagagaaga cgcccaccga gcgttagcaa tggtccgcat catattgtaa ccattaggct
     2161 atcggattga cttaatttga gtttctttag ttatcatgag gaaaggcgct gaagagctct
     2221 cctcaaattt ggggaggatc agtaatgcat agtgtaagac taaatccaga aatcaaagcg
     2281 accacaacgc tagataaaag aaagaacatc aataacgatt attagttcca gcgcaccaac
     2341 cgctcctata tatgctcaag gtagctgtta tcggagccac gggcgcagta ggccaagaat
     2401 tcattattgc cctgaacaag cacaagtggt ttgaactgac gcaggtggcc gcatctgaaa
     2461 ggtcggctgg aaagaaatac gttgatgcaa tccgtgatcc aaattctggc attttgcgat
     2521 ggcacaatag ggaagaagtg cccgagtata tcaaggacat ggttgtcagc aaagtggacg
     2581 acatcgagcc agagaatttt gaccttatat ttactgcact tgaatcagat gacgcaaaaa
     2641 taatagagcc caagttcgct aagaccacac ccgtcatcag tactgctgct gcattccgct
     2701 atgaaactga tgtaccaata ttgataccag gcattaacga taatcatgct gaactactca
     2761 acatccagag aaagaaccgc agttggaagg ggtttattgc gccactgcca aactgcacga
     2821 caaccggcat ggcaataaca ctaaagccga tacttgatag gtttggtatc gacagagtat
     2881 tcatgacctc tatgcaagcc
//
LOCUS       DASQTF010000080         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_290826,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000080 DASQTF010000000
VERSION     DASQTF010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290826"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VE308_01945"
     CDS             complement(<1..291)
                     /locus_tag="VE308_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZA99764.1"
                     /translation="MFAPTSIVAAVGSDMPAEHIRALSSKGIDTKGIIIIQGSKTFHY
                     DSSFDYDLSNRTTNKTELGVIAGFDPVIPEEYIKSDYVYLANNDPKQNIKILQ"
     gene            248..454
                     /locus_tag="VE308_01950"
     CDS             248..454
                     /locus_tag="VE308_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99765.1"
                     /translation="MSDPTAATIEVGANMLDETEAKVAAPPSILISVRKGVRVVSSAT
                     VPKTVSNAYLVANVVSLLNACRHH"
     gene            483..1043
                     /locus_tag="VE308_01955"
     CDS             483..1043
                     /locus_tag="VE308_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZA99766.1"
                     /translation="MRLVIAITGASGVIYGIRTLEVLNRLKVDTHLIITEWGGKNIKI
                     ETDKTTDYVKSLATRYYEDYNMAAPVSSGSFRVDGMIVIPCSTKTLASIANALDDSLV
                     SRAAGVCIKEQRKLVLVPRETPLSKIHLKNMTKLAGTNAVILPAMPGFYHRPKTMDDL
                     INHLVGKVLDQFNIDHGLFKRWGGGE"
     gene            complement(1198..1794)
                     /locus_tag="VE308_01960"
     CDS             complement(1198..1794)
                     /locus_tag="VE308_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZA99767.1"
                     /translation="MSFQQTVKISKERIGVLIGKGGKVKQQIEERCGVKIEIDSETGD
                     TMIIGNSSADRLEAFRAVEVITAISRGFSPQRAYLLFDDEELIFQQIDLHDYTGKSPN
                     ALERIKGRIIGEGGKARRMIEELSGSYVSVYGHTVCLIGSFREIKLTTEAISMLAKGS
                     MHKTVYNMLQSAKRKDKIDKMRLWEEKENPDKITFSDS"
     gene            complement(1794..2624)
                     /locus_tag="VE308_01965"
     CDS             complement(1794..2624)
                     /locus_tag="VE308_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HZA99768.1"
                     /translation="MLSEEEPDEQQLPREEKSKAAAQADRWLSKYERKSRFLNKMSED
                     YDVFDNVFDLPTLMTIKELRSDGVIQYIESSLAAGKESKVYLAVALDGSLRIVKIYLT
                     VSAEFKKRMQYIAGDPRFSDIKQDSRSLIMTWARKEFKNMRTAHAVGVRVPLPIAIKK
                     NVLVMEFVGDSEGNPMPALINTEEITLSDYQQVIEQMTILYQKAKLVHADLSEYNIFK
                     TDLGIMLFDFGSAIDIRHPNSKRFLFRDVTNINKFFEKRGIEVLNTDQIVEKIRGDTK
                     "
     gene            3163..3465
                     /locus_tag="VE308_01970"
     CDS             3163..3465
                     /locus_tag="VE308_01970"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZA99769.1"
                     /translation="MRGRWASAAIGAGMARRSAGRQMDQQSQAHEAEMQRAQQQIDAQ
                     QRQLEALQQQKQQPQPQPQQQEDLTQKLIKYGELKQQGLITEEEFQKLKADMLSKI"
BASE COUNT         1023 a          774 c          720 g          981 t
ORIGIN      
        1 ctgcaggatc ttgatattct gctttggatc attgtttgca agatacacgt aatcagactt
       61 gatatattct tcagggatga caggatcaaa gccggctatg actccaagct cagtcttatt
      121 agtagtcctg tttgacagat cgtaatcaaa tgaagaatca taatgaaatg tcttgctccc
      181 ttgtatgatg atgatccctt ttgtatctat gccctttgag ctgagtgccc ttatatgttc
      241 agctggcatg tctgacccta ctgctgccac tatcgaggtg ggggcaaaca tgcttgatga
      301 aacagaggca aaggtggcag caccgcctag tattctgatc tctgtccgaa aaggagtacg
      361 tgttgtgtct agtgccactg tgccaaagac cgtcagcaac gcctatttgg tagccaacgt
      421 agtttcacta ttaaatgctt gcaggcacca ttaaatagaa agcaaggtaa ccacacattt
      481 gcttgcggct agtcattgcg attactggcg cttccggtgt catttacggc ataaggacac
      541 ttgaggttct caacaggctg aaagtcgata cacaccttat tatcacagaa tgggggggca
      601 agaacatcaa gatagagacc gacaagacaa ccgactatgt taagtcgctt gcaacgaggt
      661 attatgaaga ctacaatatg gccgcacctg tttcaagtgg ctcgtttaga gttgacggca
      721 tgatcgtcat tccctgtagc actaaaacac tggctagcat tgcaaatgcc cttgacgaca
      781 gcttagtctc aagagcagca ggcgtttgca taaaggaaca acgcaaactt gttctggtgc
      841 caagggagac ccctctgtca aaaatacacc taaaaaatat gaccaagctt gcaggtacta
      901 acgcagtaat tctgcccgcc atgccaggat tctatcacag accaaagacg atggatgatc
      961 tgataaacca tctggtaggt aaggtacttg atcaattcaa tattgaccat ggtcttttca
     1021 aacgctgggg cggaggtgag tagagagaaa aataacaagg gcgaggcata aggattcata
     1081 atcttgcagc actagtagtt taggatatgc cagtgtaagc caaaccggca tacatatagc
     1141 ttgatactga aacagaatgc aatcgatcgg ttgacttata gcctcaccat gcttacatta
     1201 agagtcagag aacgtaattt tgtcgggatt ttctttttct tcccacaggc gcatcttgtc
     1261 gatcttgtct tttctctttg cactctgcag catattgtag acggttttgt gcatgctacc
     1321 cttggcgagc atggatatcg cttcagttgt aagcttgatc tccctaaagc tgccgataag
     1381 gcatacagta tgaccgtaga cagatacata agagccactc aattcttcaa tcattctacg
     1441 cgccttgcct ccttcgccaa ttatcctgcc cttgatgcgc tcaagggcgt ttggagactt
     1501 gccagtgtag tcatgcaaat cgatctgctg gaatattagc tcttcatcgt caaagagcag
     1561 gtaagctcgt tgtggcgaga atcctcttga aatggcggtt atcacttcga ccgctcgaaa
     1621 agcttccagc cgatcagcag agctgttgcc tatgatcata gtatcaccag tttcgctgtc
     1681 aatctcgatt ttgacgccac atctttcttc aatttgctgc tttacttttc ctcctttgcc
     1741 aatcaggaca cctatcctct cctttgagat cttaactgtt tgctggaagc tcattatttt
     1801 gtgtcacctc taatcttttc tactatttga tcagtgttga ggacttcaat tccccgcttt
     1861 tcaaagaact tgtttatgtt cgtgacatcg cggaataaaa atcgtttgga atttgggtgt
     1921 cgaatatcta tagcagatcc aaagtcaaac aacatgatgc cgaggtcagt cttgaaaatg
     1981 ttgtactctg acaagtcagc atgcaccagt ttggcttttt ggtacaggat agtcatctgc
     2041 tcgatcacct gttgatagtc gctcaatgtg atctcttctg tatttattag tgcaggcata
     2101 ggattccctt cgctatcccc tacaaactcc attacaagca cattcttctt gattgctatc
     2161 ggcaaaggta cccttactcc tacggcatgc gctgtccgca tgttcttgaa ctcctttcga
     2221 gcccatgtca ttataaggct gcgagaatcc tgctttatgt cagaaaatct aggatcacca
     2281 gcgatgtatt gcatccgctt tttgaactca gcgcttactg taagatatat tttgactatt
     2341 cgaagtgagc catctagcgc aactgcaaga tatactttag attctttacc cgcagcaagt
     2401 gagctctcaa tatactgtat aactccatcg ctcctcagtt cttttatcgt catgagagta
     2461 ggcaggtcaa atacattatc gaaaacatcg taatcttcag acatcttgtt taggaacctg
     2521 cttttgcgtt catattttga gagccatcta tcagcttggg ctgcggcctt gctcttctcc
     2581 tctcttggca gctgctgctc gtcaggctcc tcttctgaca acatgcaaaa gttagtgctc
     2641 tcttttatta tatgtgtaat gctacgctag gcaatatatt caacataaaa gcaccagata
     2701 acagacttat cctccttctc caatcacagc gatgcttacc tgaaagtctg cctatgatag
     2761 aaaaaccagg gagggcaatg cagatacaat gtaagactgt ttgagatgaa gaagtagaga
     2821 agaaaaagca aaagacacac taattgacat agaaaacaaa atcaataaaa gatatggtaa
     2881 taacctagca cctgtacttc tagacattag aatatgtttc tatatcactt tcacttttta
     2941 ggggtatgat aaaatcaaca tagaaattta tgaaatacat acaatttaga tactttaggg
     3001 agcaaattac atgcttgctg acgaaattat acacttgctt atgtatagaa aaccacgatc
     3061 gcatgctcat atacagtata tgagggatta gatcttttgc tgagttgaca ggcgcgagct
     3121 cgctctcata aagtataata acttgctacc ttatagaaaa ttatgagagg aagatgggct
     3181 tcagcagcta taggagcagg tatggccaga agaagtgccg gacgtcaaat ggatcaacag
     3241 tctcaagcac acgaagctga aatgcaacga gcacaacaac aaatagatgc acagcaaaga
     3301 cagctcgaag cactacaaca acaaaaacaa caacctcaac ctcaaccaca gcagcaggaa
     3361 gaccttactc agaagttgat aaaatatggt gagctgaaac aacaagggtt gataacagaa
     3421 gaagaatttc aaaaactcaa ggctgacatg ctatcaaaga tttaaactat aaggctgcaa
     3481 ctagatgaac ggctaacc
//
LOCUS       DASQTF010000081         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_570518,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000081 DASQTF010000000
VERSION     DASQTF010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570518"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VE308_01975"
     CDS             <1..589
                     /locus_tag="VE308_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZA99770.1"
                     /translation="INGLSRLDTRNIDKDHTVCIIGDGPIGLIHLRLFGLIGARTIVV
                     GRIDSRMKAAKSMGADAVLQVNDNPELTARVVSDLTNGIGASTIIVAASNPSALTLAT
                     KIASKNAQISLFAGMPKDTPFALDANLLHYNQISVIGNFSATPDSMRQAIKLVSDNRI
                     ALSEVISHYYSLSDIEDALTATESFRGLRIVINRF"
     gene            complement(675..1448)
                     /gene="cobM"
                     /locus_tag="VE308_01980"
     CDS             complement(675..1448)
                     /gene="cobM"
                     /locus_tag="VE308_01980"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HZA99771.1"
                     /translation="MVDSDTVYFVGCGPGDPELITLKAMYLMQNADVIVYSGSLVNPR
                     ILEYAKKNAVLYDASLIDRQEIGRILSESTKKGKLSIRLHDGDPGIFSTIREQIDRLE
                     DDGLKCKVVPGVTSLLAAAAEMNLELTLPGITQTLIITRAELRTPVPAGERISDLSRH
                     RGTLVFYLSVHLIESITNELLEANAYSKDTPAAVVYRAGWEDQMIIKGTLENITRKVK
                     QARVIKTALIIVGDVLAPRKYEYSKVYDEAFTHGFRRGI"
     gene            complement(1438..2163)
                     /gene="cobI"
                     /locus_tag="VE308_01985"
     CDS             complement(1438..2163)
                     /gene="cobI"
                     /locus_tag="VE308_01985"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HZA99772.1"
                     /translation="MEIVCIGCGPGDPDLVTVKAATFLKNADTIFAPTSREGRPSIAL
                     SIVRKYLSASVETVNLIFPMVKDKGKLESYWRMNADQIARAAESGKKVIYLTVGDPSL
                     YSTWIYIQREIISRYPQIHFKIVPGVPSIFAFAAQAKISLVEGDETLGIIPACYDLDK
                     IKRTASSCDTLVFLKDGRYFRDVIKMLGDAGFADESVITIAQDVSAGEEILKRHSLKD
                     LRQNREMTEKYFSLMVAKRKDGR"
     gene            complement(2225..2812)
                     /gene="cbiT"
                     /locus_tag="VE308_01990"
     CDS             complement(2225..2812)
                     /gene="cbiT"
                     /locus_tag="VE308_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HZA99773.1"
                     /translation="MFWDCRTPGIPDELFETDPDVPITKEEIRAIVLGKLRLKEGFAA
                     IDIGCGSGSITVELCLQTRSNNIYAIDFNKKAIELTARNLNKFGVEANMITAKAEDVL
                     PALPRVEAIMIGGSWGNIHRILTLATDKLVKGGRIVIDTILIETLYKTIGALKKSRLC
                     GIDITQVTLAKSRQVSTGTMMLARNTVTVISATKP"
     gene            complement(2839..3135)
                     /locus_tag="VE308_01995"
     CDS             complement(2839..3135)
                     /locus_tag="VE308_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99774.1"
                     /translation="METLDRVFYVRAITGLIAGVIVGLVIGPGTNQATAVGIALVISM
                     VFYIISYAAARRISGSIPKKERRKVATSGIFPFIFLLLMFMIVTYTMLHQDIAS"
BASE COUNT          935 a          805 c          690 g          995 t
ORIGIN      
        1 tatcaacggt ctctctcgcc tagatacgag gaacatcgac aaagatcata cagtttgtat
       61 aataggagac ggtcctatcg gtttaattca tttaaggctt tttggcctaa taggagctag
      121 aacaattgtt gtgggaagaa ttgactctcg catgaaagca gccaagtcca tgggagctga
      181 tgctgtgctc caagtaaatg acaacccaga attgactgcg agggttgtat ctgatctcac
      241 aaatggaata ggtgctagca caattatagt cgctgctagc aatcctagcg ctttgacgct
      301 ggccacgaag atagcaagca aaaacgcaca gatcagttta tttgccggca tgccgaagga
      361 cactccgttt gctctggacg ccaacttgct tcactacaac caaatctctg taataggcaa
      421 ttttagcgct acaccagatt cgatgcggca ggcgatcaaa ttagtctccg ataatcggat
      481 agctctatcg gaggtaataa gtcattacta ttccctgtcc gacatagaag atgcgcttac
      541 agccacggag agctttcgcg gtcttcgaat tgtcataaac agattctgaa tactaagacg
      601 tagccgagcc gcgaggttac actgcaatta gaagacttga cttgcctcag gctcggcacg
      661 aatgatctgt caaatcatat tcctcttcta aagccatggg taaaagcctc gtcgtaaacc
      721 ttagaatatt catactttct tggcgccagc acatcgccca cgataatcaa tgctgtcttg
      781 atgaccctag cctgcttaac ttttctggtg atattttcta gagttccctt gattatcatc
      841 tggtcttccc aaccggctct ataaaccact gcagcaggag tgtccttact ataagcattg
      901 gcttccagta gttcatttgt aatactttct atcaagtgaa cgctaagata aaaaaccagc
      961 gtaccgcggt gtctcgaaag atcggaaatt ctttctccgg cgggcacagg ggttcgtagt
     1021 tcagctcgtg ttataattaa cgtttgagta attccgggga gtgtgagctc taaattcatt
     1081 tcggccgctg cagccagtaa tgaggtaaca cccggaacaa ctttgcattt tagaccgtcg
     1141 tcttctaatc tgtcaatctg ctccctaatt gtgctaaata taccaggatc accgtcgtgc
     1201 agacggatcg agagcttacc cttttttgtt gattcagata gtatccggcc tatttcttgc
     1261 ctgtcgataa gagaagcatc ataaagaaca gcattcttct tagcatactc taaaatcctt
     1321 ggattaacca gagatccaga atacacaata acgtcggcat tctgcattaa atacattgcc
     1381 tttagcgtga ttagttccgg gtcgcccgga ccgcaaccca caaagtatac tgtgtcgcta
     1441 tctaccatct tttctttttg ccaccataag cgagaaatat ttttcagtca tctctctgtt
     1501 ctgccgcaaa tcctttagac tatgtctctt caatatttct tcccctgccg aaacatcttg
     1561 ggcgatagtt ataacggatt catctgcaaa tccggcatct cctagcatct tgatgacgtc
     1621 tctgaaatac ctgccatcct taaggaaaac gagtgtatca caagatgaag cagtacgttt
     1681 tatcttgtca aggtcataac acgcaggaat tatgccaagc gtttcgtctc cttccacaag
     1741 gctaatctta gcctgagcgg cgaaagcaaa tatggatggt actccaggaa caattttgaa
     1801 atgaatttga ggatatcttg aaattatctc cctttgaata tatatccacg tgctgtataa
     1861 cgagggatca ccaaccgtca ggtatattac cttcttgccg ctttcagctg ctcttgctat
     1921 ttggtcagca ttcatcctcc aataactttc taattttccc ttatccttga ccatgggaaa
     1981 gatcaaattc acagtctcta cagatgcact cagatatttc cgaacgattg acaaggctat
     2041 gctcggcctt ccttcgcgtg aagtcggagc aaatatcgtg tcagcatttt tcaagaaggt
     2101 tgcggctttg acggtgacaa gatcagggtc gcccgggccg cacccgatgc acacaatttc
     2161 catttagatg acctcgatgt ttcaactatt taaatcgaag tatctgcgag acagtgaaat
     2221 atacttaggg cttggtagca gatatcaccg taacagtatt cctcgcaagc atcatagttc
     2281 ctgtactcac ctgacggctc ttggctagag ttacttgtgt gatgtcgatt ccgcatagtc
     2341 tggatttctt caaggcgccg attgtcttat acagcgtttc gatgaggatt gtatcgatca
     2401 ctattctacc acctttaact aatttgtcgg tagccaaggt cagaattcga tggatgttcc
     2461 cccaagagcc tccgatcatg attgcttcaa cgcgagggag agcgggcaat acatcttctg
     2521 ctttagcggt tatcatgttt gcctccaccc cgaacttgtt aagatttctg gctgtcaact
     2581 caattgcctt cttattaaaa tctatagcat aaatgttatt gcttcttgtc tgaaggcaga
     2641 gttcaactgt aatgcttccg cttccacaac ctatgtcgat ggcagcgaat ccttccttca
     2701 gtctcaattt accaagtacg atagcacgta tttcctcctt agttatggga acatctggat
     2761 ccgtttcgaa aagttcatcc ggaataccag gtgttcgaca gtcccaaaac aatgaatgaa
     2821 actactcatc agtcatattt aacttgcgat atcttgatgt aacatcgtgt acgtgacaat
     2881 cataaacatc agcaacaaaa atatgaacgg aaaaatcccg ctggttgcca cttttcgtcg
     2941 ttcttttttg ggaatgctac cagatattct cctggccgca gcatatgaga ttatgtaaaa
     3001 aaccattgaa attacaagag cgattcctac agcagtggct tggttggtgc ctggacctat
     3061 aactaggcca acgataactc cagcaattaa accagtgatt gccctgacgt aaaaaactct
     3121 gtcaagtgtt tccaattaga taggcttcag tgccatatgc cctgtttaat tccttgcggt
     3181 cacatcaaga ctagccgttg tgattaatat tagtgagtca tgcttccaga tctcatggag
     3241 ctttcaggcg tcgaactgag tttcctcatt aatgaaatta aagcaaaggt tacacgagga
     3301 tactatgtga gcgcaatcac ccctataacc ggaaactcgt tcctctttaa gttgcaccat
     3361 acaactgaac cagacatcat gcttatgatt gcaaccatgg gtatctggat tacaagacta
     3421 aagtt
//
LOCUS       DASQTF010000082         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1034377,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000082 DASQTF010000000
VERSION     DASQTF010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034377"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..612
                     /locus_tag="VE308_02000"
     CDS             136..612
                     /locus_tag="VE308_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99775.1"
                     /translation="MTTNSKHILSVGLAAATLLAVLPFFAGSVLLLQPSLGQLAARIG
                     LVLGAGPAWFVPVVPISAIVLASGAFAVSWNQRSYLVAGLLAASGIIFTLAALIATGY
                     FAVIAVPGPILGVIIGLGIFGLGVAKSIRTARAPVAAVYLRGGVLTDNKESLREAR"
     gene            614..976
                     /locus_tag="VE308_02005"
     CDS             614..976
                     /locus_tag="VE308_02005"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZA99776.1"
                     /translation="MSEQESNRIRRIVGWGIVGLIVVIGLWIAVSLYFAPQRPGGFDY
                     SFFFPFHFGWLGAIFLIFLVFWIAKWLIWPWRGGYYSYPHKQRVDAASIVRERYARGE
                     ITKEQFEQMLRDLRQGVG"
     gene            complement(1091..1444)
                     /locus_tag="VE308_02010"
     CDS             complement(1091..1444)
                     /locus_tag="VE308_02010"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HZA99777.1"
                     /translation="MTSKWGMMLADDQVIEQVKTELMSWPGVTSQPHRFGGTEFRING
                     REMGHIHGGRFADLPFSMSIRNELVKDGRALPHHVFPNSGWVTFLINEEADITLLINL
                     FRMQYERLSRSRKPT"
     gene            1812..2153
                     /locus_tag="VE308_02015"
     CDS             1812..2153
                     /locus_tag="VE308_02015"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZA99778.1"
                     /translation="MVTVKNMVVQPSFVSAHILATYIQIEKKMRNRSRIELASNILEA
                     ANGGATKTKIMYKAFLSYAQLQEYLAMLTENSLLLRDGGIYKPTSKGIGFLQTTRQLN
                     GLLEVQASQQK"
     gene            2476..3159
                     /locus_tag="VE308_02020"
     CDS             2476..3159
                     /locus_tag="VE308_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZA99779.1"
                     /translation="MATDNNNNNNDNIIIRNITSEDIHDIVELQKQSFPIMAKDGVIW
                     HKHHLESHIRIFPEGQFCAELDGKIIASSSSLVTSLRPDYQEHTWKQATAYGMFTNHT
                     LKGDSLYGADISTHPDSRGLGIATMLYDARKDLVVKLNLRRMIAGGRLFNYCDYAKNM
                     SAQEYAEKVVRGELKDLVLSFQLNNGFKFIKILPNYMKDSRSLNYASFIEWINPKYVS
                     QKNELMLKS"
     gene            3156..>3608
                     /locus_tag="VE308_02025"
     CDS             3156..>3608
                     /locus_tag="VE308_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZA99780.1"
                     /translation="MRVIDCHVHLNRYEGTKYVPLEERIEMLVHTMEANNVNHSIILS
                     SYKVNEDRPSTEQLIEATKKYDDMLSIVAGFSIDGHNDDDFQNCRKWCKDGLIKGMKL
                     YCGYEHYYPYDKRYQRVYDLCVEFGMPVMIHTGDTFSSKGKLRFSHPLN"
BASE COUNT         1074 a          717 c          789 g         1028 t
ORIGIN      
        1 gcatgcctat cactagcaac tatcatgcac ccttcctaaa atggcttctt ggccgagcta
       61 cattaagggt gtggtaacta tgttacgtgt catgggtcaa caagtgataa aatagatcta
      121 gccaaagatt ccgccatgac aacaaattca aaacacatat tgtcagtagg cctggcagcg
      181 gcaacactct tggcagttct gccttttttt gccggcagtg tattgttgct gcagccgtcg
      241 cttggacagc tggccgcacg gatcgggctt gtactggggg ctggaccggc atggttcgta
      301 ccggtagttc ccataagcgc tattgtgctg gcttcaggag catttgctgt gtcgtggaac
      361 cagagatcat atctggtagc aggattacta gctgcaagtg gcatcatatt cacgttagcc
      421 gcactaatag ctacaggata ctttgcagtc atagcggttc cgggccccat acttggagta
      481 attatcgggc tgggcatctt cgggcttgga gtagcaaaga gcataagaac ggcaagggct
      541 cccgtagcag cagtctatct aagaggagga gttctgacgg acaataagga aagtctgaga
      601 gaggcccgat aatatgagtg aacaagaatc aaatcgcatt aggcgaattg taggctgggg
      661 catagtagga ttaatagtgg taataggact atggattgcc gtatccctct attttgcacc
      721 ccagcggcca ggaggcttcg attattcgtt cttcttccca tttcattttg gctggctagg
      781 tgccatcttc ctaatattcc tagttttctg gatagcgaaa tggttgatct ggccttggag
      841 aggaggctat tactcgtacc cacataagca acgagtggat gcagcatcaa ttgtcagaga
      901 gagatacgcg agaggcgaaa taacaaaaga gcagtttgaa cagatgctgc gcgacttgcg
      961 gcaaggggtc ggctgaaggc cttgtgtgat tggccccatt atcaagaatt aatgccatgg
     1021 ttcgattgct atatcaaaaa gaacgctgac ttaggctctt ccttcgaagc cctcatgctc
     1081 caggaagaat ttatgttggc tttctgctcc tactgagtct ttcatactgc atacgaaata
     1141 ggttgattag caatgtaatg tctgcctctt cattaatcaa aaaggtaacc cagccggaat
     1201 tggggaatac atggtgaggc aatgcccttc catctttgac caattcgttt cggatgctca
     1261 tcgaaaaagg aaggtcagca aatcttccac catgaatatg gcccatttct cttccattga
     1321 tgcgaaactc tgttccacca aaccgatgag gttgtgaagt gactcccggc cagctcatca
     1381 attccgtttt gacctgctcg attacttgat catctgctaa catcattccc cattttgatg
     1441 tcatgatgag tgttcttggg tgacatacat ctattaagga tagtagtaat ttcccactaa
     1501 cttagttatt attttctcat gactcctaat cgctaggagc tccggtctct ccctctaact
     1561 attttttact ccaacaaccc tttgttcaag tactgtagaa agatggggcc ggcaactata
     1621 ctcttttctt cgtggtcagt gggctcgtgg tagtatttct tcaaagtatc aggcaatcca
     1681 gtattctgag ctcaatagtt gaattaatag catatttttg ctttagcata tcatagctca
     1741 tctatgttaa taccccatac ctagttgctg tagacaaata agctttattg ctctgcttca
     1801 ctttggagaa agtggtcact gttaagaaca tggttgtaca accctctttc gtgtctgctc
     1861 atatacttgc gacgtatata cagatcgaga aaaaaatgag gaacagaagt agaatagaac
     1921 ttgcttctaa cattttagag gcagcaaacg gtggtgctac caagactaag ataatgtata
     1981 aggcatttct ttcatacgca caattacagg agtacctagc aatgttaact gaaaatagtc
     2041 ttctattgcg tgatggaggc atttacaaac caacaagtaa aggaattgga tttcttcaaa
     2101 ctactcgaca attgaatggc ttacttgaag tacaagcctc acagcaaaaa taggagtaca
     2161 acagaaagca atcattctta gtagcagaag aagcgttata ttcatggaga tagcagggac
     2221 atactgtcac tgccaccaat gtggagaaga gatatgaata cttatagcct tttcgtaatt
     2281 gtctgcttct tctggttctt ccgatccttg aatgagacag gatatgtcaa tacaaaagaa
     2341 agaatccaga tttactttta ggcaaaataa ggtagactag tcgattgcta acagatttgg
     2401 tcatctgaac taaaatttct agcatgcata cacactaatc tttgataaga tcactgcatg
     2461 attgagctgg tttaattggc gaccgataat aataataata ataatgataa catcattatt
     2521 agaaatatca cttcagaaga tattcatgat attgtggaac tgcaaaagca atcttttcct
     2581 atcatggcta aagatggggt aatttggcat aaacatcatc ttgaaagtca tatcagaata
     2641 tttccagagg gacagttttg cgcggagtta gatggaaaaa taatagcctc ttcaagcagc
     2701 cttgtgactt cactcagacc agattaccaa gaacacactt ggaaacaggc tacggcttat
     2761 gggatgttta caaatcatac tctcaaaggt gatagccttt atggagcaga tatatcgacg
     2821 caccctgact ccagaggtct aggtatcgcg actatgctct atgatgccag gaaggatttg
     2881 gttgttaaat tgaatttacg gaggatgata gcagggggaa gattattcaa ctactgtgac
     2941 tatgcaaaaa atatgtcagc tcaagaatat gctgagaagg ttgtcagggg agaattaaag
     3001 gatcttgttc tgtcctttca attaaataat ggctttaagt ttatcaaaat cctgccaaac
     3061 tatatgaaag acagtcgttc tttgaattat gcttctttca tagaatggat aaacccgaaa
     3121 tatgtgtctc aaaaaaatga gttgatgctt aaatcatgag agtaatagac tgccatgtgc
     3181 atctcaatcg ttatgaggga actaaatatg tgccactaga agagcgaata gaaatgttgg
     3241 ttcacactat ggaagccaat aacgttaacc attcaatcat cctttcatct tataaggtaa
     3301 atgaagacag accatccacc gagcaactga tagaagcgac gaagaaatat gacgatatgc
     3361 tctctattgt agcaggtttt tcaattgacg gccacaatga tgacgacttt caaaattgta
     3421 gaaaatggtg caaagacggt ctgataaaag gtatgaaact ttactgcggt tatgagcatt
     3481 actacccata tgacaagaga taccagcgag tctatgattt gtgcgtagaa tttggtatgc
     3541 ctgtcatgat acacactggt gacacatttt caagtaaagg gaagcttcgg ttctcccatc
     3601 cactgaac
//
LOCUS       DASQTF010000083         2803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1130640,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000083 DASQTF010000000
VERSION     DASQTF010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130640"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VE308_02030"
     CDS             <1..1458
                     /locus_tag="VE308_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZA99781.1"
                     /translation="VVVDAKILLREKPDLGVISKAQPESDYMDLFFNAKSVALIGASP
                     EPGKVGNSVLESLAKHEYIGKVYPVNAKGYSEIMGLKAYKSLDEIPDRVDVVVVTVDL
                     KFVPDLLRTAAKKDIHNFVIISGGGKELGGERATIEGQIRELSKQLQIRIIGPNCIGM
                     FNGENRLDCAFQGHARMIRPKKGHVAFLSQSGTVGIAFMESSDIFGMSKMISYGNRSD
                     VDEADMIWYLSEDPQTRVIGLYVEGLGDGRKFMNTAKRVIKERSKPVVVFKNGRSARG
                     AKQAASHTGSLGGSYAVVKGALDQAGIISVDSYEELTAALKALTWQPVPNGGRVAMVT
                     NGAGPIIAAIDQFERLGLQVAELSEETKKSLKDHYPSTYVIGNPCDITGSANADDYQF
                     AIQSFMDDPNIDIIMPWFVFQDDPLEESIVNVLASFQKQKKKPILVGAMGGPFTEKIS
                     EQIEDTNVPVYHSVIEWVTAASALVKWTRVRGQTK"
     gene            complement(2227..2316)
                     /locus_tag="VE308_02035"
     tRNA            complement(join(2227..2262,2278..2316))
                     /locus_tag="VE308_02035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2279..2281),aa:Arg,seq:tct)
BASE COUNT          787 a          577 c          670 g          769 t
ORIGIN      
        1 gtcgtagttg atgccaagat cctactacgc gaaaagccag atctaggagt catctcaaag
       61 gcccagccgg aatctgacta tatggatttg ttcttcaacg ccaagtcggt ggcactgatc
      121 ggcgcttcac ctgagcccgg caaggtaggc aactctgttt tggaaagctt ggcaaagcat
      181 gagtacatcg gcaaggtcta tcctgtgaac gcaaaaggtt attctgagat catgggcctc
      241 aaggcgtata agagccttga cgagatccct gacagggtag atgttgtagt cgttacggtc
      301 gatttgaagt ttgtgcccga tctactgagg actgcagcca agaaagacat ccataacttt
      361 gtcattattt ccggcggggg caaagaactt ggaggggagc gcgcaactat tgaagggcag
      421 atccgggagt tgtcaaagca gctgcagatc aggatcatcg gccccaactg cataggtatg
      481 ttcaatggtg agaaccgcct tgattgtgcg ttccaaggcc acgcaagaat gatacgtccc
      541 aaaaagggcc atgtcgcttt tctgtcacag agtggaactg tgggcatcgc attcatggag
      601 agctccgaca tatttggcat gagcaagatg atatcatacg gcaatagatc cgacgtggat
      661 gaggccgaca tgatctggta tctctctgaa gatccacaga cccgagtcat tgggctatat
      721 gttgaaggct tgggagatgg caggaagttc atgaatactg caaagagagt aattaaagag
      781 cggagcaaac ccgtcgttgt attcaaaaat ggcagatctg caagaggggc caagcaggcc
      841 gcttctcaca ctggatcact tggcggctcg tatgcagtgg tgaagggcgc acttgatcag
      901 gctggtatca tatcagtcga tagctatgaa gagcttacgg cagcgctcaa ggcactcaca
      961 tggcaaccgg tgccaaacgg gggtagagta gcgatggtaa ccaatggggc aggaccaata
     1021 attgctgcaa ttgaccagtt tgaaaggctt ggtctgcaag tagccgaatt gagtgaagag
     1081 actaaaaaat cccttaagga tcactatcca tcaacatacg tgataggtaa cccttgcgac
     1141 atcacgggat ctgcaaatgc tgatgattat cagtttgcaa tacagtcatt catggatgat
     1201 ccaaatattg acatcatcat gccttggttt gtctttcaag atgatccgct tgaagaatca
     1261 attgtaaatg ttctagcctc atttcagaag caaaagaaaa aaccaatact cgtaggcgcc
     1321 atgggaggac catttacgga aaagatctca gagcagattg aagacacaaa tgttccagtc
     1381 tatcactcgg tcattgaatg ggtgactgct gctagcgctc ttgtcaagtg gacaagagta
     1441 agagggcaaa caaagtaaca atctcccatt ttttgaatgt gtagatattg cgatctaaag
     1501 cttaagtgaa aactatggaa gctagttctt ttggctagtg tgcattgttt ttacttgaat
     1561 agtggaagat catagctgtg aaaagtatct gctacagcat ttacatcatt aattgaaggc
     1621 aatcatgatg atttaaatta gttgcaattg cattaccgat attgcagaaa tcggcagttg
     1681 aggcacggag ggcctttggt gttctattta tagcagtgtc gatagctgtt gtcattgctt
     1741 cttttcttag tgaaattgct tattttatca ccagtgttcc actttattac aatgtactaa
     1801 tttggcttgt cagctttggt gcagtatttg gcgcaaacta cacgcgtttg cgcaaagcag
     1861 taccagcaat ccttggtagg atgaaaacaa gcacggggtg gcccgcagct gcaaagatgc
     1921 tcaatgggat ttgttgggca ggaccttttg ctgcaatagt catttttcca tcattttatc
     1981 agtaccttat cctagctggc attggtcttg gcaacttgtc gacctatttg atgataaaaa
     2041 agtatagcaa tactgataat cccgagcagc taatagttgc tttagtttct cttgctgcag
     2101 taccagcggc tgtcatgatc gacagcatga tttttgcaat acgacaagat attgcagtaa
     2161 tgctgtcgag aatcttcatt ggaatcgcct acgccgctgg cggagcattt gcattactaa
     2221 ggaaagcggg cccggtgggc ttcgatccca cgacttccgg ctctctccaa tagaatttag
     2281 aaggcaggcg ctctatccat gctgagctac gggccccctc ctgcgctcta gcccctcttg
     2341 ttaatttaat gattgcgcga gttacaaaga tgtttatgta taaccgtttt acgtcaagcc
     2401 aatttgaaaa gctgtgtcag taggatgcat ggcagtttga ttaacccatc ctttatatat
     2461 aatatctctt cttcccgtaa ccagttgtac ccatttaatt tggcattcat tagcaacgta
     2521 gtaatatctc gaaacctaat acaagattca cattcgaact atacttttag tataaatcct
     2581 ataccacgaa actataatag ccagtcgacg aattctacat tgttgtgtga aagataatgg
     2641 ttgaaaatgg tagcgctgat aacgcacaag aatttatgca agagcaagtc aacaaaatgt
     2701 ttgaatggag cagtactttg aagctgccga caattggtcc tatgtatgct ttttcaaagg
     2761 attttagttc atacactaac gattttgtca ctcttggcaa ggt
//
LOCUS       DASQTF010000084         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1218507,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000084 DASQTF010000000
VERSION     DASQTF010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218507"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VE308_02040"
     CDS             complement(<1..568)
                     /locus_tag="VE308_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HZA99782.1"
                     /translation="MTDLKPKELIVKPTVAKSSLAEFLSKLKKKKNNLIVNVDPKLAG
                     ASVRTIHESEEADMVICTTFDQLKSFKSSGKSVAYFKKVTSNLDIDEIERASENGAEV
                     VIVDASNWKIIPLENIIAKLHKSKTKVFATAKSSEEVATLFAVLELGVDGVILSTDSE
                     EEVDRAQQQLTSARFLITRARIIEVKDVG"
     gene            complement(561..1343)
                     /locus_tag="VE308_02045"
     CDS             complement(561..1343)
                     /locus_tag="VE308_02045"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HZA99783.1"
                     /translation="MVFGRDIRLSRILNDGKMLCIPMDHGISNGPIKGLEDVHDTIYQ
                     CASAGLTCVLVNKGIIKTMPRPPDIGLIVHFSGSTSIGPAPNRKMLIGSVEEAMRLGA
                     DAVSLHINIGSKEEPEMLQKLGMISDKCDEWSLPLVAMMYPRGENIKNPHDPEIVAHA
                     ARVGAEAGADIVKTVYTGHPDSFKRVVRSCPVPVVIAGGPKANSESEILEMCNGAMAA
                     GAKGVTFGRNIFQHKNPPAMVRALRKIIIENRNVREALKELD"
BASE COUNT          555 a          528 c          421 g          676 t
ORIGIN      
        1 tgccgacgtc ttttacctct atgatcctag ctcgcgtgat gaggaacctc gcagaagtta
       61 gctgttgttg ggccctgtca acttcctctt cgctatctgt agataaaatc actccatcga
      121 ctcctagctc aaggactgca aacaatgtcg caacttcttc agagcttttt gctgttgcga
      181 acacttttgt tttagactta tgtagcttgg caataatatt ttctagaggg atgattttcc
      241 agttggatgc gtcaacgatt actacttctg ctccattctc tgatgctctt tcaatctcat
      301 cgatgtcaag gttactggtc actttcttga agtatgcaac actcttgcct gacgacttga
      361 atgatttcaa ttgatcaaaa gtcgtgcaga tcaccatgtc agcctcctcc gactcatgta
      421 ttgtacggac gctggcaccg gctagcttgg gatcaacgtt gactatcaaa ttgttcttct
      481 tcttctttag ttttgagaga aattctgcaa gagaagattt tgctacagtt ggcttgacta
      541 ttagctcttt tggtttgaga tcagtcaagt tctttcagag cctctctaac gtttctgttt
      601 tcaattatga tcttgcgtaa tgcgcgcacc attgctggag gattcttatg ctggaaaatg
      661 ttgcggccaa acgtcacgcc cttcgcgcct gctgccattg ccccattgca catctccaaa
      721 atttcgctct cgctattggc cttgggtccg ccagcaatta ccaccggtac tggacagctc
      781 ctaaccaccc gcttaaacga atcgggatga ccagtataca ctgtttttac aatatctgct
      841 ccggcctcag cacccaccct tgcagcatgc gccacgatct ctgggtcatg agggttttta
      901 atgttctcac cgcgtgggta catcatcgcg accagaggga ggctccattc gtcgcactta
      961 tcggatatca tgccaagctt ttgcagcatc tccggctctt cttttgaacc aatattaata
     1021 tgaagagaaa ccgcatcagc tccgagcctc atcgcttctt ccacacttcc tatcagcatc
     1081 tttctgtttg gtgctggacc tatggatgta ctgccagaaa agtgtactat caatccaata
     1141 tctggcggcc gtggcatggt cttaatgatg cctttgttga caaggacaca ggtgaggcca
     1201 gcagaggcgc attgatagat cgtgtcgtga acgtcttcta gccccttaat aggaccattg
     1261 cttataccat ggtccattgg aatgcacagc attttgccgt catttaatat cctcgaaagc
     1321 ctgatgtccc tgccaaagac catatttgta tccagacttt cttgctaacc tacttataat
     1381 ttgttttatc cctacaatag ttgaaataag ggcttggaag tattggtcct cgctgacctc
     1441 accactttac gtgctagtat atattacaga gctaggtcct aattaaataa tcattgcctg
     1501 ctcgctagaa tttattatct gtgcaatcgt tgacaattat tgttccaagc tacagcaaag
     1561 ctcagcgata gactaagtcc ataactttaa ccatgaacat ggcaatgtta gatcacgtag
     1621 ttttcaatag cgaaatagat ctattaattt tatgaagtaa agttatggcc actctcagga
     1681 gattacttgt aatctatttc tagctgtctg cagatagcta tgcgactgac gctggatccc
     1741 agtcgcctca acatctcaaa cacacgcaca gtctgctctc agcggttacg tctcagtcct
     1801 atgtacatat tctttacccg ttctttctaa tccacctgtg cattaaggca ggcataattt
     1861 atctcaagct tattattgtc aaaataatag ctacaggttt tagaaaggtc tcagacaggg
     1921 attcatagac atacagaata ttgaacaatt ttgacctaaa aattgggtag cttattctgt
     1981 ctattcttag ggatgctgag aaccaagctc ttatatttgc agtcatatac tctgataacg
     2041 taacttgcta aaaaagcatg aacgaaaccc gttctgctat atctgcaacc tgttctatac
     2101 acccgaatat ctgccagttc atctcaaagc agatcacaca aaggaagaat tagcggctca
     2161 tctcctgcta gcatacttcg
//
LOCUS       DASQTF010000085         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1035882,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000085 DASQTF010000000
VERSION     DASQTF010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035882"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VE308_02050"
     CDS             <1..896
                     /locus_tag="VE308_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99784.1"
                     /translation="RLSYDLRTCYIFISSSLSHIPLSEKDLESLGWNDLVAQKRQLTS
                     ELKELSDRIIDIDRNQFRSMGNAIKEQRSLLDAHTERLKQIRSEVDSRNSELLSVSEK
                     ISQSRNFLSMMEARIPSEKEEDLQSIMQKNQDIIDAKDYKSEREKNEILSKIKDATMK
                     LEATKATRTIREQFSQLTQQSVNINNAIKQLNHERDLLRSKISNINAALDNLYDSKRK
                     LASEHQSYLVRYDSIAKQFDTINARLDSMSEMRRKQRKEYGYVEQSDALFKVKEEAKK
                     KLQAGSKLSFEELKLLYGDKD"
     gene            940..1272
                     /locus_tag="VE308_02055"
     CDS             940..1272
                     /locus_tag="VE308_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99785.1"
                     /translation="MGINDFAFACSVDGSIVGYFTYHGQTMLIVQSEQEAKAGLNNFL
                     KIAEPFMEPLISHESIHVIIKKLEGTETSDSLDEVEVIVERGGIKLQVPLNFIHYSRD
                     NSGIVLPF"
     gene            complement(1306..1578)
                     /locus_tag="VE308_02060"
     CDS             complement(1306..1578)
                     /locus_tag="VE308_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99786.1"
                     /translation="MQTRKNNPQVPDIDEERDIELENIIGNLMIAQYQVDKLRSRLSE
                     RVGGYGSAGNKKASAHIFKHNEKWYKVTIRVDEMAISQREMSDDIK"
     gene            complement(1568..1720)
                     /locus_tag="VE308_02065"
     CDS             complement(1568..1720)
                     /locus_tag="VE308_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99787.1"
                     /translation="MSLLIEEEEEQTEGVPVCGANLSSPVVDAAYYIDEIHYSVGNRM
                     YIENAD"
     gene            complement(1828..2583)
                     /locus_tag="VE308_02070"
     CDS             complement(1828..2583)
                     /locus_tag="VE308_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99788.1"
                     /translation="MNERDLKSLFCSSLRANHVKNNDLIPTSEIRIVQEAHCRSFDLL
                     IASVTKEPDRHDYSQEDNILVRTQLLKHFARQEKCRIDCIRFYPVEVKSDDDVLDERL
                     PKQILDAILAFGLSILVLDKNHSKLARGLGKFLPATLICYTGIDDHFEVVSRFDRLIS
                     SGVLNLKKTFLAKALEGTNTGSKAYSRLVALEHIFQKIIFNQIYFENVGMTERELEFL
                     QMITGIIPPPSQERKKLSKLIKETANAKMTDYL"
     gene            complement(2622..>3018)
                     /gene="trpA"
                     /locus_tag="VE308_02075"
     CDS             complement(2622..>3018)
                     /gene="trpA"
                     /locus_tag="VE308_02075"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZA99789.1"
                     /translation="YSLMASKLNLATVFLVSPNTPDERLRKIVASTTGFVYVVSVYGI
                     TGARSSFQKYSLNTIRAVKQVAVTKVPVAVGFGISTPAHAKSMIAAGAEAVIVGSAII
                     NKISDGSGKRMLQDLQNFARSIKKACKNE"
BASE COUNT          879 a          635 c          619 g          885 t
ORIGIN      
        1 acagactctc ctatgatctt cgcacctgct atatttttat atcatcctct ttaagccata
       61 ttccgttgtc ggagaaagat ttggagtctc ttgggtggaa tgatcttgtt gcgcaaaagc
      121 gccagcttac cagcgaactc aaagagctat cggacaggat tattgacatt gacaggaacc
      181 aattccgctc gatgggcaac gccatcaagg agcagagaag ccttctcgat gctcacactg
      241 aaaggctaaa gcaaatacgc tctgaagtag atagtcgtaa ctctgagctt ttgtcggtca
      301 gcgaaaaaat atcacaatca aggaactttc tctcaatgat ggaggctcgg attccttcag
      361 aaaaagaaga ggatttgcag tcaataatgc aaaagaatca agatataatc gatgccaaag
      421 actacaagag tgaacgcgaa aagaacgaga ttttatccaa aataaaggac gccacaatga
      481 aactagaagc cacaaaggcc acgcgtacga ttagagaaca gttttctcaa ctgacccagc
      541 aatctgtcaa catcaacaac gctataaagc agctaaatca tgagcgcgac ttgcttcgaa
      601 gcaagatatc taacatcaat gccgcactgg ataacttata tgattcaaaa agaaagctag
      661 cctcagagca tcaatcttat ctcgtaagat acgatagcat cgcaaagcag ttcgatacaa
      721 tcaacgcacg gctagattca atgtctgaaa tgaggcgcaa gcaaagaaaa gagtacggct
      781 atgtcgagca aagtgacgcg cttttcaaag taaaggaaga agcaaagaaa aagctgcaag
      841 caggatcgaa gcttagtttc gaagaattga agcttctcta cggggataaa gattaaaatc
      901 tgatttgctc gacattcaag taattaacta ggtcataaca tggggataaa tgattttgcc
      961 tttgcttgtt ctgttgacgg ctcaatagtt ggatatttta catatcatgg gcagacaatg
     1021 ttaattgtac agtccgagca ggaagcaaag gcaggtctta acaactttct caagatcgca
     1081 gagcctttca tggaacctct catctctcat gaatcaattc atgtaattat aaaaaaatta
     1141 gagggtactg aaacatctga ctcccttgat gaagtcgaag tgattgtaga acggggtggc
     1201 ataaagctac aggtgccact gaattttata cactattcac gcgacaattc tggaattgtc
     1261 cttccatttt aagaaaaaaa tttggtgcgc tggtagtcac gcgcgttatt tgatatcatc
     1321 agacatctcg cgttggctta ttgccatctc atcgacccga attgttactt tataccattt
     1381 ttcgttgtgt ttgaaaatgt gtgcagacgc ctttttgttg ccggcagagc cgtatcctcc
     1441 gacacgctcg ctcagcctag agcgcaactt atcaacttga tattgcgcta tcatcaggtt
     1501 tcctattatg ttctctaact ctatatcccg ctcttcgtct atgtcaggaa cttgggggtt
     1561 gttctttcta gtctgcattc tcaatgtaca tcctgtttcc tacactgtaa tggatttcgt
     1621 caatgtagta cgcagcatcg actactggtg atgacaggtt tgcaccgcac accggcactc
     1681 cttctgtttg ttcttcttct tcttctatta gaaggctcat ttgagtaata gtcaaacaca
     1741 tcaaataata aaggatagta tgaatgtact ctaagaccta aagaaagact atttctagtt
     1801 gaatggaaca gttggaaaag ttaaggccta caagtaatca gtcatctttg cgtttgctgt
     1861 ttcttttatc agctttgata actttttcct ttcttgcgat ggcggtggta taataccagt
     1921 tatcatttgt aaaaattcca actctcgttc agtcatgcct acattctcaa aatagatctg
     1981 attgaaaatg attttttgaa atatgtgctc tagtgcgaca aggcggctat aggctttact
     2041 gccagtatta gtaccctcaa gtgccttagc caagaaggtc tttttaagat tgagaacacc
     2101 gcttgagata agacgatcaa accttgaaac gacttcaaaa tggtcatcga tgccagtata
     2161 gcatattaga gtagctggca ggaatttgcc aagaccgcgg gccagttttg aatggttctt
     2221 gtcaagcacc aatatagaca gcccaaaggc caatattgca tctagtatct gctttggcag
     2281 tcgctcgtct aatacgtcat catctgactt gacttcgaca ggataaaatc gaatgcaatc
     2341 tattctgcac ttttcttggc gagcaaagtg tttgagtagt tgtgttcgga ccaggatgtt
     2401 gtcctcctgg ctatagtcat gtctgtcagg ttcctttgtc actgatgcga tcaaaaggtc
     2461 aaagctgcga cagtgagcct cttgaactat ccttatttca gacgttggta taagatcatt
     2521 attcttgaca tgatttgctc taagcgagct acaaaatagc gatttgagat ctctctcatt
     2581 cattgcatag ttttttgcag cttctgttaa tgtattttgt cctactcgtt tttgcaggcc
     2641 ttttttattg accttgcaaa attctgcaga tcctggagca tccttttccc tgagccatcc
     2701 gatattttat taatgatggc actacctaca ataactgcct ctgcgccagc agctatcata
     2761 gatttagcat gcgccggcgt gcttattcca aagcctacag ctaccgggac ttttgttacg
     2821 gcaacctgct taactgccct gattgtgttt agactgtatt tttgaaaaga actgcgggca
     2881 ccagttatac cgtaaactga tacaacatat acgaagcccg tagtgctggc aacaattttg
     2941 cgcagccttt cgtctggtgt atttggagat acaaggaata cagtagcaag gttaagcttt
     3001 gaagccatta gcgaatat
//
LOCUS       DASQTF010000086         6341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_662663,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000086 DASQTF010000000
VERSION     DASQTF010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662663"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VE308_02080"
     CDS             <1..1225
                     /locus_tag="VE308_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99790.1"
                     /translation="VEEKVKDAATEVKSSVGYELGQASKAVKEKMSDAGEAVMNVDVE
                     GAARQAAGGIKDLAQTGVETVKEKMGAAQSSTEAGLSVENMTKFEKESKQSSTETDLG
                     SYEGKYPKGFFNKTVVVNNRPIGRVAKETDEVIVVFSDTDSSIRFDIPKSEISAAGAS
                     VIANEDLLFRYRTHRDAPMPEGRALRASVQEIRAAASEQLEIEKKSTTADSIMEEGRQ
                     LATVPRPETTSVSTPEGYIDTESEMSKKMKGALAELKEIIVSGTRVAKKKARQAQETA
                     AEKRAEMDAEAISRMGDLAMRFADSFEDVLSEIRTKTYAEQVQIYTGFIKLVDQQRTL
                     LVARRDFAERFKDSINVPVVEPGDMETRELEAPSELPEELEGNRSLPERKSTTTTTTR
                     RRKRAGSSKTTRRKA"
     gene            1455..2933
                     /locus_tag="VE308_02085"
     CDS             1455..2933
                     /locus_tag="VE308_02085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HZA99791.1"
                     /translation="MQNEVSNALSYATSKGFQIHPNAFAMLKGLDVDVLKIIQEIIKI
                     KKPTKNAVIVVDDIKSLIGPKKQEGPIEQTCTVLIDPTPKVTTAEGVEGYTALFRSRF
                     EKTMRILAQRPDSKRISKVSAVKQRVRMGMQIERGEKSLHGAASSIIVAGLLMSKRTK
                     KNDVELSIDDHTGILTSVSTVEDVKKQASTLALDQMVMLELENGKGRPGLTIKGIMMP
                     DIPDHLPNKSKSEAYAVLISDLHVGSKYFMEREFLRFLDWLSSSSDDDIVKKIKFLCV
                     GGDLIDGIGIFPNQDKELLEIDAAKQMSHAAQLLAKVPKHIKVLVIPGNHDLGRRALP
                     QPAIPRKDLGEQFYGLENYMMLGNPAFVELNGVKVLMYHGQSLDDIIATTPGLSYSKP
                     SEAMKMLLKARHLAPIYGERTPIAPEQEDMMVITEVPDIFHSGHVHVVDVQSYKGTLV
                     VNSGAWQSQTKYQQTMGITPTPGVAVIVNLATLQPVVYDFNQ"
     gene            complement(2923..4131)
                     /locus_tag="VE308_02090"
     CDS             complement(2923..4131)
                     /locus_tag="VE308_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZA99792.1"
                     /translation="MNSDIDNIFTKAASGRSLVKNRQALTIDYIPEKLPFRDEETMAL
                     AQVLSTIFKGARPSNLLLFGKPGTGKTAAARSVVDRLAKKASELMTDVIVIFVNAKIA
                     GGSAYKVIFEIAEELGINKEQEKQVHFTGLSMGEATDRVLNFIQRKKLNLVLVIDEID
                     SLVDRSGDDILYNFTRANQRMKGGFITLIGISNSLTFKDKLDPRVRSSLSEEEIVFNP
                     YTVDQLRQILQERAKLSFNEGSISEGAVNLCAAIAGREHGDARKAIDLLRVAAELAER
                     EGTTKVDEKHVRASEDRIERDAPFVALKNAPAHHKLVILAIIKSTNGTTGKVYDIYLS
                     LCKQTEQKPLTQRRMTQIINELDLLGIVSTDIVNQGRYGRSQKIKVMIPLTTVEEALK
                     DDINLTSLTD"
     gene            4674..5129
                     /locus_tag="VE308_02095"
     CDS             4674..5129
                     /locus_tag="VE308_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99793.1"
                     /translation="MNSGKKRFKIELEDAEGSKYNLSLEGNISKEKIMKIVEFMDLIN
                     IESNGNNESETYRSSRATPASVGDKIWSLVEGKFPYGGFTSTDILELYEDQYNEPIKL
                     SIISTYLSRYAERGRLIRTRQGKEWIYKMTRQQPMQPQPSSSTSLDLHL"
     gene            complement(5068..5652)
                     /locus_tag="VE308_02100"
     CDS             complement(5068..5652)
                     /locus_tag="VE308_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99794.1"
                     /translation="MTIDLTFSYSDFVIFGVIAFLVGMASMTFYSRLKSAFKEGRYKT
                     NEAVVEAVVLVYTRKLQDYDKIIAELRAKVDIIESRAQPHEVVSQHTSQPQPHVAPVS
                     EPVTVTQHAIIEKEVNSTTDYILKLLVERPRSSREVQLAIGRTREHTARLMKKLHDLG
                     FVNRDINTKPFRYDITDAGREMLKKRAEAALVAV"
     gene            complement(5706..6080)
                     /locus_tag="VE308_02105"
     CDS             complement(5706..6080)
                     /locus_tag="VE308_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99795.1"
                     /translation="MAAPGSNSSTLYAYIVANSLFTPRQLSIILKQLGGGGKPEKLSS
                     GAYYRQVKQCRKKVVSVLYSMMLLQSIGVVQQEAPATLSKLAEQSAVIFASERSSDAT
                     PRLNVNDVIPVINEVIKRMSKL"
BASE COUNT         1872 a         1300 c         1426 g         1743 t
ORIGIN      
        1 agtggaggaa aaggttaaag atgctgctac tgaggtcaag agctctgtcg gttatgagtt
       61 gggacaggct tccaaggcag tcaaggaaaa gatgagtgat gcaggtgaag ccgtaatgaa
      121 tgttgacgta gaaggagcag ctagacaagc agcaggtgga atcaaggatt tggctcaaac
      181 aggagttgaa acagtaaaag agaaaatggg agcagctcag agcagtacag aagcagggct
      241 cagtgtagag aacatgacga aatttgaaaa agagtcgaag caatcatcta cagagactga
      301 tctaggtagc tatgagggca aatatccaaa gggcttcttt aacaaaacag tagtcgtgaa
      361 taatcgtccg ataggacgtg ttgcaaaaga aacagatgag gttattgtcg tgttcagtga
      421 caccgatagt tcaataaggt ttgatattcc aaaatcggag atatctgcag caggggcttc
      481 tgtcattgca aacgaagacc tgctgttccg atacagaact cacagagatg ctccaatgcc
      541 tgagggcaga gctttaagag cttcagtgca agaaatacgg gcggcagctt cagaacagtt
      601 agaaattgaa aagaagagta cgactgcgga ctcaataatg gaagaaggaa ggcagcttgc
      661 aacagtgcca agacctgaaa cgacttctgt gtcaacgccg gaaggatata tcgacactga
      721 gtcagaaatg tcaaagaaga tgaagggtgc gcttgcagag ctcaaagaga taatagtgtc
      781 agggaccaga gttgccaaga agaaagccag acaagcacaa gaaaccgccg cagaaaagcg
      841 tgcggaaatg gacgcagagg cgatatcgag aatgggtgac cttgcaatga gatttgcaga
      901 ttcatttgag gatgtccttt ctgagataag gacaaagaca tatgcggaac aagtgcagat
      961 ttacaccggc tttataaagc tcgttgacca gcagcgcaca ttgttggttg cacgtcgaga
     1021 ctttgccgag aggtttaagg actcaattaa tgtccctgta gtcgaacctg gagacatgga
     1081 gactcgcgag ttggaagcgc catctgagct gccagaggag cttgagggta acagaagtct
     1141 acctgagcgc aagagcacaa ctacaaccac tacaagaaga aggaagcgag caggcagtag
     1201 taagacaaca agaagaaaag cctagtagtg taagcgcgag ttttcgtaga actgccgatc
     1261 ctcttcggcc atcactactt gcttgtgaca attttcacac agtagatacc tagtgttggc
     1321 aggcaacact ttcttgcacc tgccgcagaa tttcttcaac aatgatgata acgcgcgctt
     1381 ctaagataaa accatgacaa cagaaatcta attaaggagg atattcgaca tcaaaatggc
     1441 cgactgtatt ggctttgcaa aatgaagttt ccaacgcctt atcttatgca accagtaagg
     1501 gatttcaaat acatcctaat gcgtttgcca tgttaaaggg tctagatgtc gatgtgctca
     1561 agataattca ggaaataatc aagattaaaa agccgacaaa aaatgcagtc atagtagttg
     1621 acgacatcaa aagtttaata gggccaaaaa aacaggaggg tccaatcgag caaacgtgca
     1681 cggtgctcat cgatcctaca cccaaggtta ctactgcaga gggggtagag ggctatacag
     1741 cattgtttcg tagtagattt gagaagacga tgcgcatttt ggcccagcgg ccagacagca
     1801 aacgcatatc aaaggtgtcg gctgtcaaac agagagtcag gatgggcatg cagattgaaa
     1861 gaggcgaaaa aagcctccat ggcgccgcta gttcgattat agttgccggc ctcttaatgt
     1921 caaagcgcac gaagaaaaat gatgttgaac tatcaatcga cgatcataca gggatcctaa
     1981 cctcggtttc tacggtagaa gatgtaaaaa aacaggcctc aactcttgcg cttgaccaaa
     2041 tggtgatgct tgagcttgaa aatggaaaag gaaggccagg gctgacgatc aagggtatca
     2101 tgatgccaga cattcctgat catttgccca ataagagcaa gtcagaagcg tatgcggtgt
     2161 tgatctctga cttgcacgtt ggcagtaagt attttatgga aagggagttt ttgaggtttt
     2221 tagactggtt gtcgtcatcg tctgatgacg acattgtcaa gaagatcaag ttcctttgtg
     2281 ttggaggtga tctgattgac ggaattggta tatttccaaa tcaggacaaa gagttgttgg
     2341 aaatagacgc agcaaaacag atgagccatg ctgcacagtt actggcaaag gttcctaagc
     2401 acatcaaagt attggtgata ccgggtaatc atgaccttgg gaggagggca cttccacaac
     2461 ctgcaatccc aaggaaggat ctgggcgagc agttttacgg tcttgaaaac tatatgatgc
     2521 ttggtaatcc tgcttttgtt gaactaaatg gcgtcaaggt gttgatgtat cacggccaga
     2581 gccttgacga catcatcgca acaacaccag ggctgagcta ttccaagccc tcagaggcga
     2641 tgaaaatgct tctcaaggct cgccaccttg ctccgatata tggcgagcgc acacctatag
     2701 ccccagagca agaggacatg atggttatta ccgaggtgcc tgatattttt cactcaggcc
     2761 atgtacacgt cgttgatgtg cagagctaca aaggcaccct tgttgtaaat tcaggtgcgt
     2821 ggcagagtca gacaaaatat cagcagacaa tgggtataac accgacgcct ggcgtggctg
     2881 tgatagtcaa tttagcaaca cttcaacccg ttgtgtatga tttcaatcag taagtgatgt
     2941 aagattgata tcgtctttga gagcttcttc aactgtcgtt aatggtatca taactttgat
     3001 cttttgagac ctaccgtagc ggccttggtt aactatatcc gtcgaaacta taccgagtaa
     3061 atccaactcg tttattatct gggtcatacg cctctgagta agcggttttt gttctgtctg
     3121 tttgcaaagt gacaagtaaa tatcatatac ctttccagtt gttccatttg ttgattttat
     3181 gatagcaaga atgacaagtt tatgatgcgc tggcgcgttc tttaatgcga caaaaggtgc
     3241 atctctttca atcctatctt cgctggcccg cacatgtttt tcatcgactt tggtggtacc
     3301 ttctctttct gcaagctcag ccgcaactct caacaagtcg attgcttttc ttgcgtcgcc
     3361 atgctctctc cccgctattg ctgcacacag gttaactgcc ccttcagaga ttgagccttc
     3421 attgaaggac agtttggcgc gctcttgcaa tatctgacgt agttgatcta ctgtgtatgg
     3481 attgaagact atttcctcct cgctgaggct acttcttact cgtggatcga gtttgtcctt
     3541 gaatgtaagg ctgttagaga ttcctataag tgtaataaag ccacccttca ttctctggtt
     3601 ggcccttgta aaattgtaaa ggatatcatc accactcctg tctacaagtg aatctatctc
     3661 atctatcacc aagacaaggt ttagtttttt cctttgtatg aaattcaaga ccctgtctgt
     3721 tgcttcgccc atcgacaggc cggtaaagtg tacttgtttt tcctgctctt tgttgattcc
     3781 aagctcttct gcaatttcaa aaatcacctt ataggcgcta cctccggcga tctttgcatt
     3841 tacaaaaata actatgacat ctgtcatgag ctcgcttgct ttttttgcta gtctatctac
     3901 gacgcttctt gctgctgcag tcttgccagt tccaggcttg ccaaataata gaaggtttga
     3961 cggcctagca cctttaaaaa ttgtagataa tacttgggca agtgccatgg tctcttcgtc
     4021 tctgaaaggc aatttttccg gaatgtaatc gatagtcagg gcttgacgat tcttaacaag
     4081 tgacctgcca cttgccgctt tggtgaaaat gttatcaata tcagagttca attattatct
     4141 atcacctcac tcctgtgtgc aattttaacc cgccgccccc tgtttttctt ttgcagaata
     4201 aaagtttgtt aactttttct taacaatgat attaacaagt catttttaaa ctacaaaatc
     4261 aattataatt aactattata tcatcttgat gatatgagtt aatgtagtct tagagaaggg
     4321 tctgcagaag aaagaaaggg gtggcggcct gctagtatat ttccacctaa ttaactatgt
     4381 tagcagtatt agaacatcga tataagaaaa gacctaaaat taaccccttt atttccactg
     4441 gagcgttaac gttggcactg atactgttaa caacactaat tggaacccct ctatttccac
     4501 tggaacagag atctgataat tctttactac tagattagtt aactgaatat ttttgttaac
     4561 caaaattgtc tctctctcat acacggttaa catcagctaa gctgtttgtt atcaacgttg
     4621 gcatagctta tgttaagtgt taagacttaa caagttaagt cacctttacg cctatgaact
     4681 ctggaaagaa gcggttcaag atagaactgg aggatgcaga aggaagtaag tataacctct
     4741 ctctagaagg caatatatca aaagagaaga taatgaaaat agtcgagttc atggatctca
     4801 taaacattga gtcaaatggg aataacgaaa gtgagacata taggagtagc agagcaactc
     4861 ctgcctcagt tggcgacaag atatggagtc ttgttgaggg caagttccca tatggaggat
     4921 ttacttctac tgatatcctc gaactgtatg aggatcagta caatgaacct atcaaactaa
     4981 gtattatctc aacgtatctt tcgagatatg ctgaacgtgg tcggctcata agaacccgac
     5041 aagggaaaga gtggatctat aaaatgacta gacagcaacc aatgcagcct cagccctctt
     5101 cttcaacatc tctcgacctg catctgtaat gtcatagcgg aagggtttag tgttaatgtc
     5161 cctattaaca aaacctaagt catgaagttt cttcatcagt cgggcagtat gttctcttgt
     5221 cctgcctata gcaagttgga cttctcttga tgatcgcggt ctttcaacca gcaatttcaa
     5281 tatataatca gttgtactat taacttcctt ctcaattatg gcatgttgcg tgactgttac
     5341 aggctcactc acgggcgcta cgtgaggttg cggctgtgat gtatgttgtg acactacttc
     5401 atgtggttgg gccctactct ctataatgtc gaccttggcc cgaagctctg caattatttt
     5461 atcgtagtcc tgtagtttgc gagtatacac aagaacgact gcttcaacaa ctgcttcatt
     5521 ggtcttatat cgaccttctt taaatgcgga ttttaaccta ctatagaatg tcatactcgc
     5581 catccctacc agaaacgcaa tgactccaaa tatcacaaag tcactatatg agaaagtcag
     5641 gtcgatagtc atgtcacggt attgcttcaa aataccgtga tttaatactt tagaataacc
     5701 ttcttttaca atttggacat tctttttatc acttcattta tcacaggtat cacatcattt
     5761 acgtttaatc taggtgtagc atcactgctt ctttctgaag caaatatcac agctgactgt
     5821 tctgctagct tgcttaatgt tgctggtgct tcctgctgga caactcctat tgattgcaaa
     5881 agcatcatac tatagaggac ggaaacaacc ttcttgcggc actgcttgac ctgtctataa
     5941 tatgctcctg aactgagctt ttcaggcttg ccgccccctc ctaactgctt caaaattatt
     6001 gaaagttgtc gtggtgtaaa tagagaattt gcaacaatat atgcataaag cgtgctagaa
     6061 ttagatcctg gagcagccat agaaactata cagatttgta taggaggagc agcagtatta
     6121 gaatgttggt agcatcaaac tttaaaatgg catcttccct ttaagaggag gtggtgacag
     6181 taggaaaggt cgaacaatac cttcagaatg agataaagaa tcacggcaca atttgccttc
     6241 cgcttattga ttctgaaaac ccgacagatg caacatcgat tgcaaaaaca gtagaggaaa
     6301 gaggagcttc tgcgatcctc gtcggtggat cgtctgccgt t
//
LOCUS       DASQTF010000087         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_106435,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000087 DASQTF010000000
VERSION     DASQTF010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106435"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VE308_02110"
     CDS             <1..187
                     /locus_tag="VE308_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99796.1"
                     /translation="LKYEIGLISTLPHYYSKTKLGFATYSGTKDILQKLQEKHGKNFR
                     TLVLSNADITLLKPKAG"
     gene            complement(168..1328)
                     /locus_tag="VE308_02115"
     CDS             complement(168..1328)
                     /locus_tag="VE308_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZA99797.1"
                     /translation="MATTFLNSDFSRVVPIVSSFFTLKDTQMNIREDYIEFLVADTNI
                     KDKFPILLKELSKIGMVPTARRQKYFSRLMPALSSAIRLQIEDGIVIAVFKVQKQKIG
                     AKRKLPLPLIFFVIAVTFVFYDGFIRSSSIFAKTYVSDPVLLAGAYTMSLIGILGIHE
                     LGHMMALKHYNIKASWPYFIPGVPGVIPTFGALITQKSNMPNKNVMFDVGIAGPVAGL
                     IITIIVSIYGSAISTLIPLDEYQRLSEENRLFSFNTSLLMIATLHMTDKVVENMVLVM
                     SPLLFAAWIGFALTSINLVPAWQLDGGHLARAMFGERWHRIITYAGIGALFLMGFWPM
                     AILVLILSQRIPSNAPLDDVTSLSQRRKMLFFITLGIVIVTAPIPSSILPWA"
     gene            1425..1922
                     /locus_tag="VE308_02120"
     CDS             1425..1922
                     /locus_tag="VE308_02120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZA99798.1"
                     /translation="MQSALRKVGDYVIRRCEASDLPVVIDINMAALPEHYSDYFFESI
                     LRELPEAFIVAELNGKIVGYIMCKIEFGFSNFRKLGFVKKGHVVSVAVLEEHRGKSIG
                     KALMVEGINGAMYRKADEIYLEVRVSNTSAIKMYQKLRFEIKSRLRSYYRDGEDAYLM
                     ALELS"
     gene            1956..2177
                     /locus_tag="VE308_02125"
     CDS             1956..2177
                     /locus_tag="VE308_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99799.1"
                     /translation="MNVKRKGSKGAGAALLFVSIAVFVAYAYLLLGTEWSMLTLQFTA
                     LAAIAILLAVLGWIGYTMLTAPREEEKPG"
     gene            complement(2143..2994)
                     /locus_tag="VE308_02130"
     CDS             complement(2143..2994)
                     /locus_tag="VE308_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HZA99800.1"
                     /translation="MLSTILSSDEVSQVYSAFDQIGDIVIIKIPDGLMPKKKLIADTI
                     LAHVKTAKVVFAQVSPVKGDYRVRNLEFVAGENRTITEYKEHGCRFRVDVAKTFFSPR
                     LSTERLRIANMVGEGETIVNMFAGVGTYSILMSRVNKTCKVYSIDSNAVAAELCEANA
                     KLNKVEDRVVSIHGDAGKVIKDKLAGQADRVLMPLPERAKEFVDSAVLALNKKGVVHY
                     FLHIKADNKKTGQELGLQDAHNAFAKYNHIVQQVRVVRGVGPRIYQIVADVSVKTSPA
                     FPLPSVQ"
     gene            complement(3016..>4017)
                     /locus_tag="VE308_02135"
     CDS             complement(3016..>4017)
                     /locus_tag="VE308_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZA99801.1"
                     /translation="KNVMVVEHDMTLLDYLSDYVHIIYGEPGAYGIVSGMQSTKVGIN
                     NFLEGFLPTENVRFRDKAFRFDITSSNEDVPLDIPIASYGNLTKSFPSFKLNVAGGKI
                     RRGGIIGIIGANALGKTTFMRMLAGVDKPDSGKIDVGATISYKPQYLNQEYDGDVRSL
                     MYMAHLNPIEGSSVEQQIIIPMGVKKLYEKNVKNLSGGELQKVAVAASLLRQADIYAL
                     DEPSAFLDAEDRIAVARFLQRFIRAQGKSAIIIDHDIQLIDLVADSLVIFEGQPGLEG
                     SATEPIKKEEGMNRFLKALSITYRRDETTGRPRVNKEGGRLDREQKESGNYYYTKTQ"
BASE COUNT         1077 a          901 c          897 g         1142 t
ORIGIN      
        1 gctgaaatat gaaatagggc ttatctctac tcttccccat tattactcca agacaaaact
       61 tggttttgcg acctactctg gcacaaagga catcttgcaa aaattgcaag aaaaacatgg
      121 caagaacttt aggacacttg tattatcaaa cgcagacata accctgctca agcccaaggc
      181 aggatagagc ttgggatagg tgcagttact atgacgatgc caagggttat gaaaaagagc
      241 atcttgcgcc tttgggatag tgatgtgaca tcatctaagg gtgcattact agggatcctc
      301 tgactaagta tcagaactag tattgccata ggccaaaacc ccatcaggaa cagcgctcct
      361 atgcctgcat aggttataat tctgtgccag cgctctccaa acatagcacg agcaagatgc
      421 ccaccgtcaa gctgccaagc agggacgagg ttgatggacg taagtgcaaa tcctatccag
      481 gcagcaaaaa gtaggggcga cattactaga accatgttct ccacaacctt gtcggtcata
      541 tggagtgtag caatcatgag caggctagta ttgaatgaaa atagcctgtt ttcctcagat
      601 agcctctggt attcatctag aggtatgaga gtagatatag cagagccata gatcgataca
      661 attatcgtta ttatcaaccc tgcaactggt ccagcaatgc ctacatcaaa catgacattt
      721 ttgttgggca tgttgctctt ctgggttatc aaggcgccaa atgtcggtat aacgccggga
      781 acaccgggta taaaatatgg ccatgatgcc ttgatattat aatgtttgag agccatcata
      841 tgcccaagct catgtatacc cagtatgcct atgagcgaca tcgtgtaagc tcctgcaagc
      901 aggacaggat cgcttacata ggtctttgca aatatactgc ttgagcgtat gaagccgtcg
      961 tagaatacaa aggtgacggc aattacaaag aatatgagag gcaaaggcag ttttcttttg
     1021 gctccaatct tttgtttctg aaccttaaat acggcgataa ctatgccgtc ctctatctgg
     1081 agccttattg cggacgacaa ggcaggcatc agtcttgaga aatatttttg cctcctggca
     1141 gtgggaacca tgccgatctt gctcagctct ttgagcaaga tcgggaactt atccttgatg
     1201 ttagtatcgg ctactaggaa ctcaatgtag tcttccctga tgttcatctg tgtatctttt
     1261 aatgtaaaga atgacgaaac tattggaact acacgagaaa aatcactgtt caggaaagta
     1321 gtagccactg aagatttctt ttgagttaat ccaattaaaa tattgttgtt ctgcagagga
     1381 tatgtaaggt taaatagtgt gtttctgttg ttgtagttgt tactttgcaa tcagctctgc
     1441 gaaaagtagg cgactatgtt atacgccgct gcgaagcaag tgatctccca gtagtaatag
     1501 atattaacat ggccgctctg ccagagcact actcagacta cttttttgaa tcgattctgc
     1561 gagagcttcc agaggcgttc atcgttgcag agctgaacgg caaaatagtc ggctacatca
     1621 tgtgcaagat cgagtttggg ttttcaaact ttcgcaaact aggctttgtt aagaagggtc
     1681 acgttgtttc tgttgcggtc ttagaagagc atagaggtaa gagcattggc aaggcactga
     1741 tggttgaagg aattaacggc gcaatgtata gaaaagctga cgagatatac ctagaggtaa
     1801 gagtcagcaa tacaagtgca atcaagatgt atcagaaatt gcgttttgag ataaaatcca
     1861 gattgcgatc ttattacagg gatggtgagg acgcctacct gatggcgcta gagctgagtt
     1921 aaagattatt aagatgctgc aaatgcctct ccgctatgaa tgttaaaagg aagggaagca
     1981 aaggagcagg cgctgccctt ctttttgttt ctattgcagt gtttgtcgct tacgcctatt
     2041 tgctgcttgg aacagaatgg agtatgctga cactgcagtt tactgcctta gctgcaatag
     2101 caatccttct ggcggtgctt gggtggatag gatatacaat gcttactgca ccgagggaag
     2161 aggaaaagcc gggctagttt tcactgaaac atcagccact atctggtata ttcttgggcc
     2221 tactcccctt accactctaa cctgctgcac aatatggttg tattttgcaa aagcgttgtg
     2281 cgcgtcctgt aatcccagct cctggcctgt ctttttgttg tcggccttga tatgaaggaa
     2341 ataatgcaca acaccttttt tattgagtgc aagcactgca gaatcaacga actcttttgc
     2401 tctctcagga agcggcataa gcactctgtc ggcctgacct gcaagtttat ctttgattac
     2461 tttaccagca tcgccgtgaa ttgaaacaac cctgtcctct accttgttca acttagcatt
     2521 cgcttcgcac aattctgcag caacagcatt agaatcgatg ctatagactt tacacgtctt
     2581 gttcaccctt gacatcaaaa tggaataggt gcccactcct gcgaacatat tgactatggt
     2641 ttcgccttcg cccaccatgt ttgcaatcct gagcctctcc gttgagagcc taggtgaaaa
     2701 aaatgtttta gcgacatcta ctctaaacct acagccatgc tccttgtact ctgtaattgt
     2761 cctgttctcg cctgcaacaa actctaagtt gcggacacga taatcgcctt tcactggcga
     2821 gacctgagca aaaaccacct tggcagtctt aacatgagca aggatcgtgt cagctataag
     2881 cttctttttt ggcatcaggc cgtcaggtat cttgatgatt acgatatcac ctatctggtc
     2941 aaatgcggaa tatacttgtg agacttcgtc tgacgacaga atagttgata gcatgttttt
     3001 cagcatgtgt ggcatttact gagtcttcgt gtagtaatag ttgccagatt ccttttgttc
     3061 gcggtcaagc ctgccaccct ctttgttgac gcgcggcctg cccgttgttt cgtcacgcct
     3121 atatgtaatt gaaagtgcct tgagaaagcg gttcataccc tcctcttttt taatcggctc
     3181 agtggcagat ccttcaaggc ccggctgccc ttcaaatatc accaaactat cggctaccag
     3241 atcgatcagc tgtatgtcat ggtcaataat aattgctgac ttaccctgag ctcttatgaa
     3301 gcgttggagg aacctagcta ctgctatcct gtcttctgca tcaagaaatg cagacggctc
     3361 gtctagtgcg taaatatctg cttggcgcaa gagagatgct gctactgcga ccttctggag
     3421 ctcgccaccg ctcaggttct tgacattctt ttcatagagt ttctttacac ccattggaat
     3481 aatgatctgc tgttctacag atgatccttc aattgggttc agatgtgcca tatacatcag
     3541 tgaccgcacg tcaccatcat actcttggtt caggtactgc ggcttgtagg atatcgtggc
     3601 gcccacatct attttgcccg aatcaggctt gtctacgcct gccagcatac gcataaaagt
     3661 agtcttgcca agtgcattgg caccaatgat tccgattatc ccacctcttc ttatcttgcc
     3721 accggccaca ttgagtttaa aggatggaaa tgatttggtt aagttgccat agctggcaat
     3781 ggggatatcc agaggtacat cttcgtttga agaagtgata tcaaatctga atgccttgtc
     3841 tcgaaagcgc acgttttcag taggcaaaaa gccttcaagg aaattgttga tgcccacttt
     3901 tgtgctctgc atgccagaaa cgataccata tgctcccggc tcaccgtaga taatatggac
     3961 atagtctgat aggtagtcaa gaagcgtcat atcgtgctct acaaccatca cattctt
//
LOCUS       DASQTF010000088         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_663219,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000088 DASQTF010000000
VERSION     DASQTF010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663219"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            922..1278
                     /locus_tag="VE308_02140"
     CDS             922..1278
                     /locus_tag="VE308_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99802.1"
                     /translation="MPANQYMYPETPIDFASSKIISGVKNLREGLDPDVLAYWYKRVE
                     DRSKEIVPRHLRDKIHFEQDRILWMKFNLDVSKRAVPYVMQVIQEYIPMMPYSTALYF
                     TKVQEILTDEMNKELR"
     gene            complement(1275..1562)
                     /locus_tag="VE308_02145"
     CDS             complement(1275..1562)
                     /locus_tag="VE308_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99803.1"
                     /translation="MKTIEFECDDRYEAEKLASLMSVQKDNTVWVSGIEAVVGNEIVI
                     QLKDRSSHAILIRDEETVIRLSSFMTDLIKGKIKIHSSDCIGPVTRITILD"
     gene            complement(1559..>2301)
                     /gene="trpS"
                     /locus_tag="VE308_02150"
     CDS             complement(1559..>2301)
                     /gene="trpS"
                     /locus_tag="VE308_02150"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZA99804.1"
                     /translation="YTYENILDIIAIGFNPKRTKIIVNTKHIQHLYPIAIEVAKRITF
                     STVRAVFGFSNSTNIGMIGFPPIQAAPCFLPSVIEGKNVPVLIPAAIDQDPYWRMTRD
                     IADKMNYYKPAQIHSKFLPGLGMLGKMSSSKPETAIFTTDDPEIVDKKVSKAFTGGQP
                     TVALQRQLGANALGCPVFWYLRYFFDTEKQSDERMLRCKSGDLLCGECKSDLSKGAKP
                     FVVEFKKRRERAKDVIDKFMYDEKPFTK"
BASE COUNT          678 a          474 c          465 g          684 t
ORIGIN      
        1 taacctgcaa tgcccacacc cctttttggg tcaaattgat aaccatctcc tacaaatttg
       61 agcttttgct ctggatagcg cagtgtaaag cgcttgataa caagatgctt tgagccagat
      121 tcaattgatt tgatgaagcc acttgcagtt gacgaacgtg aggatgatga tgacgacata
      181 gacataatat ctctgaaata tcttgtacag tatcgccata tagacctgtg caagctactc
      241 taaacattca aacaatcgta ctaatagaat attaaatgtt aatattatat caaaaattca
      301 tatatgatat tggaggatgg gctaaaaaga tataatgctg actataatca ggctctcatc
      361 cgatgctaca tatcatattt tccaaaattc atgcagacag ggctaggtat tcaagtaaca
      421 tgaagagcga gtaacagctg cagaaacgca agatacttat cgaaacaata ccagaaaaaa
      481 agtcacgcct aaatcataag ataggaacga agaaacatga actctcttac aatttatttt
      541 ctatttggga tagctattat tgttgtttaa gatatcatcg ctttttattc tgctatttga
      601 gtcttcaatg ctttagggac atgaatagat tgagtttggt ccgccaagcg gttatatcgc
      661 atcatgaata acgagacttg actcaaagcc ctatcctatc ttaaaagcct attatggcct
      721 tgccttttcc tggttgctta tcatgcttca tgtaattgca tagttcaaat ctcatacact
      781 gccaagagca tggaacgaca ttgccagctt agacctctat agcattagaa ttaacagagc
      841 tgaagaaaat tcaaaaaaga ggcatgttga ctaatttcta tgtgatgcta agcaatcttt
      901 gattatcaaa attttattct gatgcctgca aatcaataca tgtacccaga aacacctatc
      961 gattttgctt caagcaagat catatcaggc gtaaaaaacc ttcgtgaagg gctcgatcca
     1021 gatgttcttg catactggta caagcgcgtc gaggataggt ccaaagagat cgtcccacga
     1081 catcttagag acaagattca ctttgagcaa gacaggattc tgtggatgaa atttaattta
     1141 gatgtctcaa aaagggcagt cccatatgtt atgcaggtca tccaagagta cataccaatg
     1201 atgccatact cgacggcact ctatttcaca aaagtgcagg aaatactgac ggacgagatg
     1261 aacaaagagc tacgttaatc aagaattgtt attcttgtta cagggccaat acaatcgctt
     1321 gagtggatct ttatttttcc ttttataaga tctgtcatga aagagctaag cctgattact
     1381 gtttcttcat ccctgatcaa aatagcgtgg gacgacctgt cctttaactg tatgacgatt
     1441 tcgttgccca ctactgcctc gatacctgaa acccagacag tattatcctt ctgtacggac
     1501 atgaggcttg caagcttttc cgcttcatag cggtcgtcgc attcaaactc tatagtcttc
     1561 atttcgtgaa tggtttttcg tcatacatga atttgtcgat gacatctttg gctctttctc
     1621 gccgtttttt gaattctaca acaaaaggct tggctccttt cgacaggtct gatttgcact
     1681 cgccacacaa gaggtcgccg ctcttgcatc tcagcattcg ctcatctgac tgcttttcag
     1741 tatcgaagaa gtagcgtaag taccagaaga ctgggcagcc aagggcatta gcgccaagct
     1801 gcctctgaag cgccacagtg ggttgaccgc cagtaaaagc tttagacact tttttgtcaa
     1861 ctatttctgg gtcgtctgtg gtgaaaattg cagtctcggg cttagaggat gacatcttgc
     1921 ctagcatgcc tagtcctggc aagaacttgc tatgaatctg ggcaggctta taataattca
     1981 tcttgtcggc aatgtctctt gtcatgcgcc agtatgggtc ttggtcaatt gctgcaggta
     2041 tcaatacagg tacattcttg ccttcaatca cggaaggaag gaagcaaggc gccgcctgta
     2101 taggcggaaa gcctatcatg cctatattgg tggaattgga aaacccaaat actgcccgta
     2161 cggtcgaaaa tgtgatgcgc ttagcaactt cgatcgcaat tggataaaga tgttgaatgt
     2221 gttttgtatt cacaattatc ttagttcttt tcggattgaa accaattgca atgatatcta
     2281 agatgttttc gtaggtgtag c
//
LOCUS       DASQTF010000089         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_19374,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000089 DASQTF010000000
VERSION     DASQTF010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19374"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..333)
                     /locus_tag="VE308_02155"
     CDS             complement(157..333)
                     /locus_tag="VE308_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99805.1"
                     /translation="MGQIYNVLIILAITLLMAVMTTVLAYFVDLLPQVVGDNVAQIGS
                     NNASINGSEVAQDN"
     gene            complement(412..972)
                     /locus_tag="VE308_02160"
     CDS             complement(412..972)
                     /locus_tag="VE308_02160"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZA99806.1"
                     /translation="MKIYTKAGDRGETSLIGGKHVNKADPRITAYGEVDELNSSIGLA
                     ISFLHSKQDELSDVGDILVQIQNDLFVLGSDLADPSWPQTDGKTPRTSEKMASALEPI
                     IDRIESELESITFFILPGGSVEASFLHLSRGVARRAERAVASLSKSQPINPAIIVYLN
                     RLSDLLFVTARLANKRLGMQDIPWRK"
     gene            complement(1063..1206)
                     /locus_tag="VE308_02165"
     CDS             complement(1063..1206)
                     /locus_tag="VE308_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99807.1"
                     /translation="MHRLEACKGGLRIVEGIDCEVMVLKPLFKDIWNVTFVIHYGYTF
                     MNT"
     gene            1226..1432
                     /locus_tag="VE308_02170"
     CDS             1226..1432
                     /locus_tag="VE308_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99808.1"
                     /translation="MPRINGFNLYRQLKKRDTGVKVCFLTAFQIYYEKFREMFPTIDV
                     KAFIRKPVTISNLVHQINTVIASK"
     gene            complement(1416..2108)
                     /locus_tag="VE308_02175"
     CDS             complement(1416..2108)
                     /locus_tag="VE308_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HZA99809.1"
                     /translation="MGKPIIFLGPSLSHEKARKIFDADYRPPARKGDFLRLAADFEVV
                     EMAVGFVDGVFLQDYPPTPIEVYHLARKKGVLLVGAASLGALRAVELEKFGMVGIGKI
                     FQLYKTGKLDADDEVAVTFASEEGGYQLQSEAMIDIRYNLYLAHKKGIISEKAKNVLV
                     KLAKEIYFPHRKYMYIVEEARNRYPMLESEINSFDSYIRSNRKSLKEMDAIRLVKYLK
                     EHYESTNAISTR"
     gene            complement(2093..>2464)
                     /locus_tag="VE308_02180"
     CDS             complement(2093..>2464)
                     /locus_tag="VE308_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HZA99810.1"
                     /translation="AITEVSQTRAANIQGARDDLRKIKYNDQNTDDRRAWQFMPSTKK
                     IKFPEVQTFFNEDILDDINLILSRLKGVGLNQVIIVDLTNPDIGIPVVRAIVPGLETF
                     KITKSVMGMRARACFKQWVSR"
BASE COUNT          632 a          588 c          509 g          735 t
ORIGIN      
        1 tgtcgccttc aagaaggcct gagccatggt gtatcctgtc tatgtgcttt gaaagctctt
       61 ctttgctgtc gaactttgat gcgcagtagg ggcaactaac agtatccttt tcactggcca
      121 tgcttaatgc atgagaagag atacccaact ttaaagctaa ttgtcttgag ctacctcgct
      181 gccattgatt gaggcattat tagatcctat ctgcgccaca ttgtcaccaa ctacctgcgg
      241 caacaggtcg acaaaatatg caagcactgt tgtcatcacc gccatcaaca aggtgatggc
      301 caatattatc aatacattat agatttggcc cacggcctat aacttgccag tcaagctttg
      361 gccattttaa tgttagtctt tcattgcaat tgctgacaga gtagtgataa attactttct
      421 ccaagggatg tcttgcatgc ccagcctctt gttggcaagc cgagctgtca caaaaaggag
      481 atcagatagc ctgttaagat agactattat tgcagggttt attggctggc tctttgagag
      541 cgacgcgaca gctctctctg cacgacgtgc aactcctcta ctaagatgca aaaatgatgc
      601 ttctacgctt cctccaggca ggataaaaaa tgtgatcgat tcaagctccg actcgatcct
      661 gtcaatgatt ggctctagtg cagatgccat tttttcgctg gtgcgcggcg tcttgccatc
      721 ggtctgtggc caagaagggt ctgcaaggtc tgagccgaga acaaaaaggt cattctgtat
      781 ttgcactaag atatctccta catctgaaag ttcatcctgc tttgagtgca gaaacgaaat
      841 tgcaagaccg atactagagt tcagctcgtc aacttctcca taggcggtta tgcgcggatc
      901 tgccttgttg acgtgcttgc caccgataag actagtttcg cccctgtcgc ctgccttggt
      961 gtagattttc attatccact tacagcaaaa aggagataat ttaagaatat atctgttgga
     1021 taaaaaaggg gactgttgaa ataaatagtg ccttaaaaaa ccttatgtgt tcatgaaagt
     1081 atagccatag tggatgacga acgtgacatt ccaaatgtcc ttaaaaaggg gcttgagcac
     1141 catgacttcg cagtcgatac cttcaacgat ccgcaggccg cccttgcaag cttccaacct
     1201 atgcattatg ataattgata tccgaatgcc taggataaac ggcttcaacc tgtacagaca
     1261 gttgaagaaa agggacaccg gcgttaaggt ctgcttcctg actgcgtttc agatctatta
     1321 tgaaaagttt agggagatgt ttcccacaat agatgtcaag gcgtttatca gaaagcctgt
     1381 caccatatca aacctcgtcc atcagataaa tacagttatc gcgtcgaaat agcattagta
     1441 gactcgtagt gctccttaag gtattttaca agtcttattg catccatttc tttgaggctc
     1501 tttctatttg acctaatata gctgtcaaaa gagttgatct cgctttcaag cataggatac
     1561 ctgttccttg cttcttcaac aatgtacata tattttcggt gtggaaagta aatctctttt
     1621 gccagtttga caagaacatt ttttgccttt tcgctgatga ttcctttttt gtgcgcaagg
     1681 tagagattat atcgtatatc tatcatagcc tcagattgta gctggtagcc cccctcctcc
     1741 gaggcaaatg tgactgccac ttcgtcgtct gcatcaagct tgcccgtctt gtagagttga
     1801 aatatcttgc cgattcctac cattccaaac ttttcaagct caactgccct caatgctcct
     1861 aggcttgctg caccgaccaa tagcacaccc ttctttcttg ccagatggta cacttcaatt
     1921 ggagtaggtg gatagtcctg caggaacaca ccatctacaa accccaccgc catttctact
     1981 acctcaaaat ctgcagctag ccgcaaaaag tcgccttttc ttgcaggcgg cctgtagtct
     2041 gcgtcaaaga ttttccgtgc cttctcgtgg ctcagcgaag gccctagaaa tattatcggc
     2101 ttacccattg cttaaaacac gcccttgccc gcatgcccat aactgacttt gttatcttga
     2161 aggtctcaag ccctggtact attgccctga ccactggaat tccaatatct ggatttgtta
     2221 agtcgacgat tattacctga ttcaatccta ccccctttag ccgtgagaga atcaaattga
     2281 tatcgtcgag aatatcttcg ttaaagaatg tctgtacttc tgggaatttg atcttttttg
     2341 ttgagggcat aaactgccag gcgcgtctat catcggtgtt ctgatcgtta tacttaatct
     2401 tccgtagatc atcccttgcg ccctggatgt ttgcagccct tgtctgtgat acttcagtga
     2461 ttgc
//
LOCUS       DASQTF010000090         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1316971,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000090 DASQTF010000000
VERSION     DASQTF010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316971"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VE308_02185"
     CDS             complement(<1..577)
                     /locus_tag="VE308_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HZA99811.1"
                     /translation="MFDNLREGLRGALKKIVGASDINEELIDSICKDVQRSLLQSDVN
                     VRLVISITQNLKQRALGEQPPKGLSRKDHIVTILYGELAKLLGYSGDVIKTIDKAQED
                     DPSLAMSFFKAGKQNVVLMLGIQGSGKTTVTAKMARWLTKHGYGVGVIGADTWRPGAL
                     TQLKMNCSKINVEVFGEEQNNNAVAIARNGLD"
     gene            complement(581..1270)
                     /locus_tag="VE308_02190"
     CDS             complement(581..1270)
                     /locus_tag="VE308_02190"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017178 - diphthine-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HZA99812.1"
                     /translation="MKLAALYSGGKDSTFAISCAQEMGHWVACLITMHPKADDSALFH
                     YPNSWVTEYLADAMQIPIIGFQVSGRSKEDEMKALEEAIMQVKSLYGIDGIVYGGISS
                     NYQKQAFEQVCMRQRISAVAPLWNSEPERYITELVKRGFHIIIAGVSTMGLDKEWLGR
                     ELDKEAIAKLEYMSKKCGFNLTFEGGEAETLVIDCPLYRKKLQINAATAYWDGQRGIF
                     EIRDVALVEKK"
     gene            complement(1607..2011)
                     /locus_tag="VE308_02195"
     CDS             complement(1607..2011)
                     /locus_tag="VE308_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HZA99813.1"
                     /translation="MSKPMDLGALKVGSYIIVDGEPCRIVSYDHSKPGKHGSAKARIT
                     AVGVFDSKPHPFVGPVSTKVDVPLIDKRNGQIISKQGDTLQIMDLETFETFETTAVEE
                     EIKDRIAQGEVQGRDVEYWKVLDRIKIVRIKG"
     gene            complement(2056..>2289)
                     /locus_tag="VE308_02200"
     CDS             complement(2056..>2289)
                     /locus_tag="VE308_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99814.1"
                     /translation="ARRERVKKASLAQMKLLASEQPKFTFPYSKNLLILEVEGKKTHQ
                     TDQKYCERTRRDVARMGISLNNLVSFSILEKLK"
BASE COUNT          539 a          578 c          478 g          694 t
ORIGIN      
        1 aatcaagtcc atttctggct attgctacgg cgttattatt ctgctcctct ccaaagactt
       61 caacatttat tttgctgcag ttcatcttca attgagtcag cgcgccaggg cgccaagtgt
      121 ctgcaccaat gactcctacc ccgtagccat gctttgtgag ccacctagcc atcttggcag
      181 ttacagtagt cttgccgctt ccttggatac cgagcataag gactacattt tgcttgccag
      241 ccttgaagaa cgacattgct agagaaggat catcttcctg cgccttgtca atcgtcttga
      301 tgacatcccc tgaatagccc agtagcttgg caagctcccc atacaatatt gtaacaatgt
      361 gatctttcct tgaaagcccc tttgggggct gctcgcctag tgccctttgt ttgaggttct
      421 gcgtaattga tatcactagt ctgacgttga catctgactg gagaagtgaa cgctggacat
      481 ccttgcagat cgagtcgatg agttcctcgt tgatgtcaga tgctcctact attttcttca
      541 gagctccgcg aaggccttcg cgcaggttat caaacatgta ttatttcttt tctactaatg
      601 ccacatcccg tatttcaaat attcccctct ggccgtccca gtaggcagta gccgcattga
      661 tttgaagctt tttccgatag agcgggcagt cgatgacaag tgtttcagct tcaccaccct
      721 caaatgtaag gttaaatccg cattttttgc tcatatattc cagcttggcg attgcttcct
      781 tgtcaagctc cctgccaagc cattccttgt cgagccccat tgttgatact cctgcaatta
      841 tgatgtgaaa gccacgcttg acaagttcgg tgatgtatct ttctggctca gaattccaca
      901 gaggtgcgac cgctgaaatt ctctgacgca tacatacttg ctcaaatgcc tgcttttggt
      961 agttgcttga aatgccacca taaactatgc cgtcaatccc ataaagcgac ttgacctgca
     1021 ttattgcttc ttcaagcgcc ttcatctcat cctcctttga acggccgctt acctggaatc
     1081 caattatagg aatctgcatt gcatctgcaa gatattctgt gacccaactg ttagggtagt
     1141 ggaagagagc gctgtcgtct gctttaggat gcatcgtgat aagacatgca acccagtggc
     1201 ccatttcctg tgcacaagat atcgcaaatg tgctgtcctt accaccggaa taaagcgccg
     1261 ctagcttcac aaaggcacag agatgtgcgc tcactattaa cttcttcttt gaagctagat
     1321 atctgattgt gagtctctct ctctgtgtgt gtgtgagtaa atgaggagcg ttagacgagc
     1381 atcaccctta tcctcgagtg ggtaagatgc acattattcg ggtgcatacg attccgacgt
     1441 gaccgcagag acaggaaaaa aaattctata ataacaacga tgtggatgcc tcaggatctc
     1501 ataatcttca tcatagttga tgtgtatagt cagctggctc ttccgaatat attcctcatc
     1561 agcatccatg cagcaattat aaggtctcac gtatgaactt tagcttctag cctttaatcc
     1621 tcactatctt gattctgtca agcaccttcc agtactcgac atccctgcct tgtacttcgc
     1681 cctgcgctat ccggtccttt atctcctcct ctactgcagt tgtttcaaaa gtttcaaagg
     1741 tttcaagatc cattatctgg agtgtatcgc cctgtttcga tattatctgg ccgttacgtt
     1801 tgtctatcag gggtacatcg actttcgttg acactgggcc cacaaatgga tggggtttgc
     1861 tatcaaacac ccccaccgct gtaattcttg cctttgcaga cccatgcttt ccgggcttgc
     1921 tgtggtcgta agaaacgatc ctgcatggct ctccgtcgac gattatgtac gaaccgactt
     1981 tgagtgcgcc aagatccatt ggcttgctca taaatctgaa tcaaagccta gagaaaagga
     2041 tacatattta atattttact ttagtttttc gagaatggaa aagctaacta ggttattgag
     2101 cgatataccc atacgtgcca catcgcgcct ggtacgctca cagtatttct gatccgtttg
     2161 atgcgttttc ttgccttcaa cttcaagaat tagaaggttc ttagagtatg gaaaagtgaa
     2221 ttttggctgc tctgacgcca agagcttcat ctgggcaagg ctagccttct tgaccctttc
     2281 gcgccgtgc
//
LOCUS       DASQTF010000091         5521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1038644,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000091 DASQTF010000000
VERSION     DASQTF010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038644"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VE308_02205"
     CDS             complement(<1..287)
                     /locus_tag="VE308_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HZA99815.1"
                     /translation="MSYKLASEPAEACSLCNSTSIVFDSETSEAICSSCGMVVKDNIE
                     SMGPEWRSYSGEDMESKSRTGMPSSLAFHDMGLSTFISYSNVDANGVAISA"
     gene            438..995
                     /locus_tag="VE308_02210"
     CDS             438..995
                     /locus_tag="VE308_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99816.1"
                     /translation="MKVLPENARSTSITRSFRFESDVLNVLDEEARQMGISVNALVGI
                     ILRRYAEFTRYLSKIDMVVINREVLTSLIEMLGEEELYKMGVKLGQTVLADTIMFWKK
                     ETTEKAVMEYIEKMVCRYGQLGTYDERRMPGGQMAIVVRHRLGKNGSRFLEGYLHGGL
                     KHILDIDALFEITESSVKCEIPVRR"
     gene            1055..1669
                     /locus_tag="VE308_02215"
     CDS             1055..1669
                     /locus_tag="VE308_02215"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HZA99817.1"
                     /translation="MFAIVHMSDVVRIPPNRLTHSLNDAAIQILKEKYESMISPEVGY
                     VIMITDANPSSIGKLVAGDGATYHKVTFKALAFYPKLQEIIEGEVVEITDFGAFVRIG
                     PTDALLHLSQITDDYLKSDVKQGVIVANQTARSLKIGSKIRARITAVSLGKGAGMGKI
                     GITCRQPFLGAVEWVADEIKKAKASGEQQQQSAAAKEKKGGDRK"
     gene            1669..1857
                     /gene="spt4"
                     /locus_tag="VE308_02220"
     CDS             1669..1857
                     /gene="spt4"
                     /locus_tag="VE308_02220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HZA99818.1"
                     /translation="MAKEKACRKCKVITTGKVCPNCKSTDLSVDWSGIILVFDAGNSQ
                     VAKTLDIISPNKYALKVT"
     gene            1858..2709
                     /gene="nadC"
                     /locus_tag="VE308_02225"
     CDS             1858..2709
                     /gene="nadC"
                     /locus_tag="VE308_02225"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZA99819.1"
                     /translation="MENLKAFLNVREMLANFLQEDVGAGDITSDNIIPADLAAKAEIV
                     CKSRFAVVCGLEEASLLFDICGCNSVILVKEGSKVAKGTVVMNVSGCARAILKAERTV
                     LNIVMRMSGIATETRRMVDLAKGVTILATRKTAPGLRYFDKKAVVMGGGATHRMRLDD
                     MVLIKDNHLVLLSDLGRCIRLARKNVGSSIKVECEARTKEEALAVIAAEADAVMLDNF
                     TPRQAQQAIRQITRMGLRNKVKIELSGGINQKNIRHYSRAKPDFISLGYITHSSKAID
                     FSLEITK"
     gene            complement(2699..3649)
                     /gene="nadA"
                     /locus_tag="VE308_02230"
     CDS             complement(2699..3649)
                     /gene="nadA"
                     /locus_tag="VE308_02230"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZA99820.1"
                     /translation="MTMLDLDYKHRVLELKKQRSAVVLAHNYQLPEVQDIADFVGDSL
                     ALSKKAATTESNIILFCGVHFMAETASILCPNKKVLIPDLEAGCSLASTINSRELQAW
                     KSEHPGALVVSYVNTTAEVKALSDYCCTSSNAVKVVNSIPKDREVLFLPDLFLGSYVA
                     ETTKRKNMYIWPGECHVHAGIKAEDINKMFASFKDADFLVHPECSCTSSVIYHISKGD
                     LAKNGHILSTEGMMKHARASSTKQFLVATETGILYRMQKENPDKQFIPIKSDAVCKYM
                     KKITLEKVYNSLREDIYEVKVPKHTADKARLAIDRMLAIS"
     gene            3858..4040
                     /locus_tag="VE308_02235"
     CDS             3858..4040
                     /locus_tag="VE308_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S30e"
                     /protein_id="HZA99821.1"
                     /translation="MPTHGSLTKAGKVRGQTPKIQGKPRSGPIARLRNKDNFIKRFEK
                     KRLPGQKKPERRGGRR"
     gene            4078..4911
                     /gene="nadX"
                     /locus_tag="VE308_02240"
     CDS             4078..4911
                     /gene="nadX"
                     /locus_tag="VE308_02240"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HZA99822.1"
                     /translation="MPRKSVALIGCGTIGTHLALAIDSGSIANASLIGLFDIVDGNAK
                     NLKSKLKSGPEVYLDFGRLINSPADIIIEAASQQAVRKFGKLVIQANKDLMIMSVGAL
                     ADRKFLAELFELAALREGRSRIYIPTGAIAGIDAIRSVRHLLDSVTLTTTKSPKALAG
                     APFLATREINFDRITKTTEIYEGSAVEAVKLFPANVNVAAVLSLAGIGADKTKVRVLV
                     DPKATTNQHEITATGSFGVIKITVNNVTAPGNPKTSFLAVLSAIECLRSICDDAIRIG
                     S"
     gene            4964..5215
                     /locus_tag="VE308_02245"
     CDS             4964..5215
                     /locus_tag="VE308_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HZA99823.1"
                     /translation="MSYGGGDDSFGAKPVEAGKEYDVQITEISRKGDGIARIQGFVIF
                     VKGGKVGQNAKIRISQIGPRFATAEIIDGSAASPNEPTS"
     gene            complement(5264..5458)
                     /locus_tag="VE308_02250"
     CDS             complement(5264..5458)
                     /locus_tag="VE308_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99824.1"
                     /translation="MSEYSELAAKHYRQYMWQKQKESWIHGLMALVLRILHALDKTLQ
                     QASIKKARRVEKVNVEEEDP"
BASE COUNT         1631 a         1151 c         1283 g         1456 t
ORIGIN      
        1 tcagcactta tggcgactcc atttgcgtct acgttggagt aggagatgaa agtagataat
       61 cccatatcat gaaaagcaag tgaagacggc atacctgtcc tactcttgct ttccatatcc
      121 tcgcccgagt aagacctcca ttctggcccc atcgattcaa tattatcctt aacgaccatt
      181 ccacacgacg aacagatggc ttcgcttgtt tcagaatcaa aaacaatcga agtgctatta
      241 cataaagaac aagcctctgc cggttccgat gcaagtttgt aactcatgtt gcaaacgata
      301 ggtacggaat taatttaagt cccatgtata cagtattggt gaatgcatta cagtgtagtg
      361 caagacgcta cataaaacaa tactataaca cagaatcgtt ccatttaaac gcctaatctt
      421 cctcgtaatg gacgtatatg aaagtacttc cagaaaatgc ccgctcaact tcgatcacca
      481 gatcgtttag gtttgaatct gacgtgctga acgtacttga tgaagaggca aggcagatgg
      541 ggatatccgt aaacgcgcta gtgggcatta tcttgcgtag atatgccgaa tttacccgct
      601 atctatcaaa aatagacatg gttgtgataa atcgtgaggt tcttacgtct cttattgaaa
      661 tgctaggtga agaagagctt tataagatgg gagtcaagct gggccagact gtgcttgcag
      721 acacgatcat gttttggaaa aaggaaacta ccgaaaaggc agtgatggag tatatcgaga
      781 aaatggtctg tagatatggc caacttggca cttacgatga gaggaggatg cccggaggcc
      841 agatggccat agtggtgcgc cacaggcttg gaaagaatgg atcacgcttc cttgagggat
      901 atctgcatgg gggactaaag cacatactag acatcgacgc cctatttgaa atcactgaat
      961 cgtcagtcaa atgcgaaatt cctgttagaa ggtagcaagc ccttctacaa gacaaaaagt
     1021 tcataatgac ctctccatct agaatgcctg gattatgttt gccatagtgc atatgagcga
     1081 cgtcgttaga attcctccaa accggcttac tcattctctc aatgatgcgg ccattcaaat
     1141 tctcaaggaa aaatacgaaa gcatgatcag tcccgaagtt ggatacgtca ttatgataac
     1201 agatgcaaat ccaagttcaa ttggtaagct cgtcgctggc gacggtgcga cataccacaa
     1261 agtcactttt aaggcccttg cattctatcc caagctccaa gaaataattg agggtgaagt
     1321 ggtcgaaatt actgactttg gggcctttgt aaggatagga cctactgacg cccttctcca
     1381 cctctctcag attactgatg actatcttaa aagcgatgtc aagcagggag tcattgtagc
     1441 aaatcagact gcccggtccc tcaagattgg atctaagatt agggcaagga taactgcagt
     1501 aagccttggc aagggagcag gcatgggcaa gatagggata acttgcaggc agccttttct
     1561 tggtgctgta gaatgggttg ctgatgagat aaagaaggca aaggctagcg gagaacaaca
     1621 acaacaatcg gctgctgcaa aggagaaaaa agggggagac aggaagtaat ggcaaaggag
     1681 aaagcttgca gaaagtgcaa agtgattact acgggaaaag tgtgtcccaa ctgcaagtca
     1741 actgacctaa gcgtcgactg gtcaggtatt atactggttt ttgacgctgg gaattcgcag
     1801 gttgcgaaga cgcttgatat tatcagtccc aacaaatatg cacttaaggt cacctaaatg
     1861 gaaaatctaa aggcatttct taatgtccga gaaatgcttg ctaatttttt gcaggaagac
     1921 gttggtgcgg gcgatattac aagcgataac ataattcctg cagaccttgc agccaaggca
     1981 gaaattgtat gcaaatcgcg ctttgcagtt gtctgtggcc ttgaagaagc ctccctgctt
     2041 tttgatattt gtggctgcaa tagcgtaatt cttgttaagg aagggtcaaa agtagcaaag
     2101 ggaacggtgg ttatgaatgt atcaggctgt gcaagagcta tcctcaaggc ggaaaggaca
     2161 gtgctaaata tcgtgatgag gatgagcggt atagcaactg agactaggcg catggtcgac
     2221 cttgcaaaag gcgttacaat attggcgacc cgtaagacag cgcctgggtt acgttatttt
     2281 gataaaaagg cagtggttat gggaggagga gccacccata gaatgcggct cgatgacatg
     2341 gtgctaataa aggataatca ccttgtcctt ctctctgatc ttggaagatg tattaggctt
     2401 gccagaaaaa atgtcggctc atcaataaag gttgaatgcg aagcaaggac caaggaagaa
     2461 gcattagcag tcattgcagc tgaagcggac gctgtcatgc tggacaattt tactccaaga
     2521 caggctcaac aagcgataag gcagataaca agaatggggc tgagaaacaa ggtaaagata
     2581 gagctttcag gtggcatcaa tcaaaaaaat atcagacatt actcgagggc caaacctgac
     2641 tttatttccc tcggctatat tacgcattca tccaaggcaa ttgatttcag tcttgaaatt
     2701 acgaaatagc tagcattcgg tcaattgcaa gccgcgcctt atctgcggta tgctttggca
     2761 cctttacttc gtagatatct tccctaagtg aattgtaaac cttttctaaa gtgatctttt
     2821 tcatatattt gcacactgca tcggatttga taggtatgaa ctgcttgtca gggttctcct
     2881 tctgcatgcg gtacaaaatc ccggtttcag tagctactag gaattgcttt gtactagatg
     2941 ctcttgcatg tttcatcatg ccttcagttg aaagtatgtg tccgttcttt gccaaatcac
     3001 cctttgatat gtgatatatc actgaagagg tgcaactaca ttctggatga actaaaaagt
     3061 cagcatcttt gaagcttgca aacatcttgt taatgtcctc tgccttgata ccagcatgca
     3121 cgtggcattc accaggccaa atgtacatgt tctttctctt ggttgtctcc gcgacataag
     3181 aacccagaaa caggtcaggc agaaacaata cttctctgtc tttggggatg ctatttacaa
     3241 ccttgactgc atttgaggac gtgcagcaat agtcagacaa tgcctttact tctgcagttg
     3301 tgttaacata acttacgact aatgcacctg ggtgctctga cttccaggct tgtaactcgc
     3361 gggagttaat tgtagaagca agcgagcatc cagcctcaag gtcaggtatg agaacttttt
     3421 tattcggaca tagaatcgag gctgtctcag ccataaaatg gaccccgcaa aataaaatta
     3481 tgtttgattc tgtagtggct gccttctttg ataatgcaag tgaatctcct acaaagtcag
     3541 ctatgtcttg gacttccgga agctggtaat tgtgtgccag taccacggca cttctttgtt
     3601 tctttaactc aagtacacga tgcttgtagt ctaggtctaa cattgtcatg tctgctgata
     3661 aacatgagat ataatagagc gtctacattt aaagtagtat aatgtaggac aacgctgcct
     3721 tcaagagtga atttaaaggc aatacttgcc accatcactt gtgagttgga ttgatttatg
     3781 ccatatggac tttaccatct cagactgatg catacaaata taaatattag tatatatcaa
     3841 ctacacaatc gagagaaatg ccgacacacg gatcgcttac aaaagctgga aaggtaagag
     3901 gtcagacacc gaagattcag ggtaagccgc ggtcagggcc gattgctaga cttcgcaata
     3961 aagataattt tatcaagagg tttgaaaaga agaggctgcc aggccaaaag aagccggagc
     4021 gccgcggggg cagaaggtaa aaatcacata tttacaccat atcttcagtc acgttagatg
     4081 ccaagaaaaa gtgttgcgct cataggttgc ggtactatcg gaacccatct agctctggcc
     4141 atagattcgg gtagcatagc gaatgcatcg cttattggac tctttgatat tgtcgatggc
     4201 aatgcaaaga atctaaagtc aaagttgaag agtggtccgg aagtttatct tgattttggc
     4261 cgactcatta actccccagc agacatcata attgaggccg cctcgcagca agctgtcaga
     4321 aagtttggca agctggtaat tcaagccaac aaagatctaa tgataatgag tgtaggtgcc
     4381 cttgcagata ggaagttttt agcggaattg ttcgagctag ccgctttaag ggaaggccgc
     4441 agcaggatct acataccaac tggagctata gcaggcatcg atgcaatacg tagcgtcaga
     4501 caccttctag attccgttac gcttactact acgaaaagtc ctaaggcgct tgctggtgcc
     4561 ccgttccttg ccacaagaga gatcaacttt gatagaataa caaaaaccac agagatctat
     4621 gagggctcag ccgtagaagc tgttaagttg ttccctgcaa atgtcaatgt agctgccgtt
     4681 ctgagtcttg ccggcattgg tgctgacaaa accaaggtaa gggtattagt agatccgaag
     4741 gcaactacta accagcatga aattacggca acgggtagct tcggtgttat aaaaataact
     4801 gtcaacaatg taacagcacc tggaaacccc aagacaagct tccttgctgt gctttcagct
     4861 atagaatgcc ttcgttcgat atgtgacgat gccataagga taggctcgta ataattattc
     4921 tcttcttagt ttatattggg ttttgagttg agcaactcca aatatgagct acggaggagg
     4981 agacgatagc ttcggcgcaa agcctgtgga agcaggtaaa gaatacgacg tgcagatcac
     5041 tgaaataagt cggaaaggtg acgggattgc aagaatacaa ggatttgtta tctttgtaaa
     5101 ggggggaaag gtaggtcaga acgcgaagat ccggattagc cagatcggac caaggttcgc
     5161 cacggcagaa atcatcgacg gctcggctgc ttcacccaac gaaccaacaa gttaagttac
     5221 tgcaccctga caaacgtacc cagtgacact ttttttctta tctttacgga tcttcttctt
     5281 caacatttac cttctctacc cttcgagcct tctttatgct tgcctgttgt agtgttttat
     5341 ctagcgcatg caaaatgcgc aatacaagag ccatcagccc atgaatccaa gattcttttt
     5401 gtttctgcca catgtactga cggtagtgct tcgctgcaag ctcagaatat tctgacacgc
     5461 tattagctcc cccgtctccg tgatataata gttagtcagc ccaaagaaat ttaggttatt
     5521 t
//
LOCUS       DASQTF010000092         7080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_479593,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000092 DASQTF010000000
VERSION     DASQTF010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479593"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..632
                     /locus_tag="VE308_02255"
     CDS             381..632
                     /locus_tag="VE308_02255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HZA99825.1"
                     /translation="MSSEKKILKITDHIYQPKHEVLPKAHAEEVLRKFNTKPGQLPYI
                     LQSDKGIEDLEPRPGDIIKITRKSPTSGESVYYRYVVEG"
     gene            658..3987
                     /locus_tag="VE308_02260"
     CDS             658..3987
                     /locus_tag="VE308_02260"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HZA99826.1"
                     /translation="MWPLINDILRREGVARQHLNSYNEFMERGLQSIIDEVGEIEIET
                     AEYPYKIKLGKIKLQQPRIMELDGSITHVAPVEARLRNLTYASPVMLECSIVEDGKIL
                     ESRFIHVGDMPVMAKSNACILHNLVEPKLIELGEDPRDPGGYFVINGSERVIVGLEDL
                     SYNKIIVDVEETTGALLYKAKVYSSIVGYRAKLELIMRPDGSVVAKIPGSPVDIPLIT
                     LVRALGLESDRDIANAVSLNEAIQDELEPSFEKIGEITTSRDSIVYISKRIAPGMLEE
                     FQIKRAETLLDWGLLPHLGKNPDNRHEKALFLGEATSKLIELKLGWIDTDDKDHYGNK
                     VIKFAGQMLADLFRTAFRNLIRDMKYQLERSGQKRGINAVAAAVRPGIISDKLNNAIA
                     TGNWGRGRVGVTQLLDRTNYMSTISHLRRIQSPLSRSQPNFEARDLHATHFGRICPAE
                     TPEGSNCGLVKNLALSAIISVSVPSAEIIEKMYELGVQHVDEANENLRRKGARVFVDG
                     KLIGYAENGRSTSDTLRNLRRSGKIHPHVGVYFYSSGNPNATNRLYISCNAGRVLRPL
                     AIVGEGKNLATSEIIEKISKKFLSWSDLLYMGIMELVDANEEENCYISMDVHKLESNN
                     THLEIFPSAILGVGASIIPYPEHNQSPRNTYESAMAKQSLGFSTPLMNASTYVRQHFM
                     LYPQTPTVSTKAISLLGLDDRPTGQNCVVAVLPFEGYNIEDAIVFNKSSVERGLGRTF
                     FYRVYEAEAKQYPGGMKDSFELPESDTNIRGYRGEKAYRLLEHDGAIMHESVVTGGDI
                     LIGRTSPPRFMEEYKEFEVKGPYRRDTSIGVRPSENGVVDTVIMTQSVEGGKMYKIRV
                     RDMRVPEIGDKFASRHGQKGVIGMLVNQEDLPYSEDGVVPDIMINPHAFPSRMTVGQF
                     MESLGGKAASLRGTIVDGSAFLGEKFDSIKDILEKHGFKHSGKETMYDGRTGKKFPAD
                     VYMGIVYYQKLHHMVADKIHSRARGQVQMLTKQPTEGRARGGGLRFGEMERDCLIAYG
                     ASMMLKDRLLEESDKAEINVCERCGLLSYYDVKQRRFICRVCGEKSKISSVVIAYAFK
                     LLLQEMMSLNVAPRLLAREKV"
     gene            4004..>7080
                     /locus_tag="VE308_02265"
     CDS             4004..>7080
                     /locus_tag="VE308_02265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HZA99827.1"
                     /translation="MEEPVKILGGIKFSIWSPVEVRKFSVAEVTAPETYDEDGMPVQG
                     GLMDNRLGTLEPGQKCATCGNTSARCPGHFGHIELAEPVLHIAFIDDIHKLLLITCRS
                     CNKIKLHPEELAKYKNIRDAKAAYAVITLENIKEEIIEKAKKVKVCPHCEKEQYDLIF
                     TKPTIFVEKTEAGENRLLPITIRERLVHIPDADLILLGYDPSTARPEWFVLQVLPVPP
                     VTVRPSIILETGIRSEDDLTHKLVDIIRVNQRLKESKEAGTPPLIVQDLVDLLQYHVT
                     TYFDNEVSGIPQAHHRSGRPLKTLTQRLKGKEGRFRGSLSGKRVDFSSRTVISPDPNL
                     AISNVGVPVDVAKKLTIPETISQWNIGRLREHVVNGPNTYPGANYIIRPDGVKIRLDY
                     VNDRRVLADSIAPGFMVERHLSDGDIVIFNRQPSLHRMSIMAHYVRVLPYRTFRLHPA
                     VCPPYNADFDGDEMNLHVPQSEEARAEAALLMRVQDQLVSPRYGGPIIGGIRDFITGA
                     FILTRDDTTLSREEFANLALIGGYKGNLPEPDINHNGTLLYSGKQLFSLFLPSDFNFI
                     ITSKWTKATKKEGRDVVIKAGRLTSGVIDKASIGAEEPDSVLHRIVKDYGNDRAGKFL
                     DSILVMLKTFITHRGFTYGYSDLWLSEETRKEINNVIEKAYDKVYDLIRQYNEGSLPL
                     TRGLAAEEALELYVVNELSRARDRAGRTADRAFPDDNSGIIMASTGARGSTLNIGQMT
                     AALGQQSIRGKRIQKGYHNRSLPHFKRNDANPDSKGFIKSNYRDGLSPLEFFFHAMGG
                     REGLVDTAVRTQQSGYMQRRLINALEHLKIEYDHTVRDPHGNIIQYLYGEDGVDPAKS
                     DHGEAVNISRLIESESVVDEGKKASDVEIENILSKRADDLNPKVKKHLVSAFAHNRLS
                     KNGIEKVLRKTIDLIQRALAEPGEAVGVVTAQSIGEPGTQMTLRTFHFAGVKERNVTL
                     GLPRLIELVDARKKPVTPTMDIYLDKEHRVSREKALEVAQQIIFTKVGDLILKT"
BASE COUNT         2169 a         1404 c         1750 g         1757 t
ORIGIN      
        1 ggcaattcta gtttgagtaa caatgtcgat tgagactcga tttcgcgctc cattgcttca
       61 acagatcttg gtcgggccat gttttggact ctatagcaag attcacctaa tttgtatgca
      121 gcgacaagcg cgccattttc caacgcgtcg aggaattttc ggtaagcttt acgtttcatt
      181 tatgttataa agagctccac gttggagtta gcgtatcaag agcaaattct ggctcggatg
      241 tctccgtcag ccaatatagt atacttatat atgttcgttt tattgaggta tcctatgcac
      301 aacagcgtta gggatttcat tccattgatg attagtttct attgtgagca aatatgtttt
      361 tctgagtagg agcgactgaa ttgtcatcag aaaagaaaat actaaagatt actgatcata
      421 tatatcaacc aaagcatgag gttcttccta aggctcatgc agaagaggtg ttgagaaaat
      481 tcaatacaaa accaggacaa ttgccgtata ttttgcagag cgataaaggt attgaagacc
      541 ttgaaccaag gccaggagat atcattaaaa ttactcgcaa gagtccaacg tcgggtgaaa
      601 gtgtatatta ccgatacgtt gtagaaggtt gacttctaac tatggagaaa cttgtcaatg
      661 tggcccttaa ttaacgatat tttgcgaaga gaaggtgttg caaggcagca cctaaactcc
      721 tataatgagt ttatggaacg agggctccaa agcatcatag atgaggtagg agagatcgaa
      781 atagaaaccg cagaataccc ttacaagata aaattaggca agatcaaact gcagcaacct
      841 aggataatgg aactggatgg ttctattact cacgtagcgc cggtggaagc aagattacgt
      901 aacttgacgt atgcttcacc agtcatgtta gaatgcagca tagttgaaga cggtaaaatt
      961 ttagaatcca ggtttatcca cgttggggac atgcctgtca tggccaagtc taatgcttgc
     1021 attttacaca acctggttga gcccaagctc atagaacttg gagaagatcc aagggatcca
     1081 ggtggctatt ttgttattaa tggctctgag cgtgtcatcg tggggctaga ggatctctcg
     1141 tataataaaa ttattgtgga cgtggaggaa accacaggag cactacttta caaggcaaaa
     1201 gtgtattctt cgatagtcgg gtatagagcc aaacttgagt tgataatgcg ccctgacggt
     1261 tcagtcgttg ccaaaattcc aggatctccg gtggatatac cattaataac cctagtacga
     1321 gccctgggtc ttgagtctga tagggacatc gccaatgctg tttctttaaa tgaagcaata
     1381 caggatgaac tggaaccttc atttgagaag ataggagaga ttactacgag tcgtgactcg
     1441 atagtctata tcagcaaaag aattgctcca gggatgctag aagagtttca aataaaaagg
     1501 gcagaaaccc tgcttgactg gggtcttctc cctcatctag ggaaaaatcc tgacaacaga
     1561 catgagaagg ccttgtttct tggagaagcc actagcaagt taattgagct taagctggga
     1621 tggatagata ctgacgacaa agaccactac ggaaataaag tcataaaatt cgccgggcaa
     1681 atgctcgccg atctttttag aactgcgttc agaaatctta ttagggatat gaaatatcaa
     1741 ttagaaaggt ctggccagaa acggggaata aatgcagtag cggcggcagt tagacccggt
     1801 atcatatccg ataagctgaa caatgcgata gcaacaggta actggggaag gggcagagtt
     1861 ggtgtaaccc aattgttaga caggactaac tacatgtcga ctattagcca tctaagaaga
     1921 atacaatctc cactgagcag gagtcagcct aactttgaag ccagggatct ccatgccact
     1981 cactttggga gaatttgtcc agcagaaacc cctgagggct cgaactgtgg tctcgtaaag
     2041 aacttggcgc tatcggcgat aatttctgtg agcgttccgt cggcggaaat catagaaaag
     2101 atgtatgagc tgggagtgca acacgttgac gaagcaaatg aaaatctcag aaggaaaggg
     2161 gctagagtct ttgtggatgg aaagctgatc ggttatgctg aaaatggacg aagcacatca
     2221 gacacattga ggaacctcag acgatcgggg aagatacacc cacatgtggg tgtatatttt
     2281 tactcttctg ggaatcccaa cgctacaaat aggctttaca ttagttgcaa tgcgggccga
     2341 gttcttcgac cgctggctat tgttggagaa ggaaaaaatc tagcgaccag tgagataatt
     2401 gaaaagatta gtaaaaaatt tctttcatgg tccgatcttc tatacatggg aataatggag
     2461 cttgtggacg ctaatgaaga agagaattgt tatatttcga tggatgtaca taagcttgaa
     2521 agtaacaata ctcatttaga gatctttccg tcggcaattc ttggtgtcgg ggcttctatt
     2581 atcccatatc ctgagcataa tcagtcacca agaaacacct atgaaagtgc aatggccaaa
     2641 caaagtcttg gcttttcaac tcctttgatg aacgcaagca cttatgttcg tcagcatttc
     2701 atgctttatc ctcaaactcc tacggtgagt accaaggcga ttagtctgct tggactggat
     2761 gaccgcccta caggtcaaaa ctgtgtcgtc gcggtgttac cgtttgaggg ttacaatatt
     2821 gaagatgcaa tcgttttcaa taagtcctct gttgaaagag gtctcggacg aacattcttc
     2881 tatagggtat atgaagcgga ggcaaagcaa tatccaggag ggatgaaaga cagttttgaa
     2941 cttcctgaat ctgatacgaa tatcaggggg tacagaggag aaaaagcata caggctccta
     3001 gagcatgatg gcgctatcat gcatgaatcc gttgtcacgg gcggagacat actcattggc
     3061 agaacttcac ctcctagatt tatggaggaa tataaagagt ttgaagtcaa aggtccttac
     3121 agaagggaca cgtctatagg tgttagaccc tcagaaaatg gcgttgtcga taccgttata
     3181 atgactcaat ctgttgaagg gggcaaaatg tacaagataa gggtgcgaga tatgagggta
     3241 cccgaaatag gcgacaagtt tgcttcaaga cacggtcaga agggcgtgat cggcatgtta
     3301 gtcaaccaag aggatcttcc ttattcggaa gacggggttg ttccggacat aatgataaat
     3361 cctcatgcct ttccctccag gatgacagta gggcagttta tggagtcact aggagggaaa
     3421 gctgcttctc ttcgcggaac aattgttgat ggctcagctt ttcttggaga aaagtttgac
     3481 agtataaagg atatcctaga aaaacatggc ttcaaacaca gtggcaaaga gacaatgtac
     3541 gatggacgta caggaaagaa attcccagcc gacgtgtata tgggcatcgt ttattaccag
     3601 aagctacacc acatggtggc tgacaagatt cacagtagag ccaggggaca agtccaaatg
     3661 ctcacaaaac agcccacaga aggaagagct agaggaggag gactgagatt tggtgaaatg
     3721 gaacgggact gcttgatagc atacggcgct tctatgatgc tcaaagacag attactggag
     3781 gagtccgata aagctgaaat aaatgtttgt gagaggtgtg ggctgttgtc ctactatgat
     3841 gtcaagcaac gaagattcat atgcagggtc tgcggcgaaa agtccaagat ttcatcggta
     3901 gtaattgctt atgcgttcaa gctcctgttg caagaaatga tgagtctcaa tgttgctccg
     3961 cggttgttgg ctcgggagaa agtatagggg ctgactgact agcatggaag aaccagtaaa
     4021 aatactcggt ggtataaagt tcagcatctg gtcgccagtt gaggtccgta aattcagtgt
     4081 tgctgaggtc actgctcctg aaacatacga tgaggacgga atgcctgttc agggcggact
     4141 aatggataac cgcctcggca cgctagaacc tggccagaag tgtgcaactt gcggtaacac
     4201 atctgcgaga tgtcctggac attttggtca tatcgagtta gcagaaccgg tattgcatat
     4261 cgctttcatc gatgacatac ataagctcct tttgatcacc tgcagaagct gcaataagat
     4321 caaattgcat ccagaagagc ttgcaaagta caagaatatt cgcgatgcaa aggccgccta
     4381 tgctgtaata accctagaga atatcaagga ggaaataata gaaaaggcca aaaaggtcaa
     4441 ggtctgtccg cattgcgaaa aagagcagta cgatctaatt tttaccaagc ctacaatatt
     4501 cgtagaaaag accgaagcgg gggaaaacag gctattgccc ataaccataa gggaacgctt
     4561 ggtccacatt cccgatgcag atcttatttt gctgggttat gatccctcta cggcgaggcc
     4621 agaatggttt gtattgcaag tcctgccagt ccctccggtc acagtcagac cctcaatcat
     4681 tctagagaca ggtatccgat ccgaggatga cctgactcat aagcttgtag acataatacg
     4741 cgttaatcaa aggcttaagg aaagcaagga ggccggcacg cctcctctta ttgttcaaga
     4801 tctggttgat ctgcttcagt atcacgtaac tacatacttc gataatgaag tatctggaat
     4861 acctcaggct catcacaggt ctggaagacc tctcaaaacg ctaacccaaa ggctaaaggg
     4921 taaagaaggc aggttcaggg ggtcactctc gggcaaaaga gtcgacttct ccagcaggac
     4981 cgtaatttca ccggatccga accttgctat atccaacgtt ggcgtgccgg tagatgtagc
     5041 caagaagctc accattccag aaactatctc acagtggaat atcggaaggc tgagggaaca
     5101 tgtggttaac ggtccaaaca cttatcctgg tgctaactac attattcggc ctgacggggt
     5161 caaaataagg ctggactatg tcaacgacag aagagtgtta gcagattcaa ttgcacccgg
     5221 tttcatggta gagcgacacc tttcggatgg agatatcgtc atcttcaata gacagccctc
     5281 tttgcacagg atgtcaatta tggctcacta cgttagggtc ttgccttatc gaacgtttcg
     5341 attgcatcct gctgtatgtc ctccatacaa cgcagatttt gatggggatg agatgaactt
     5401 gcacgtgccc caaagcgaag aagcccgagc ggaggcggca ttgcttatgc gtgtccaaga
     5461 tcagcttgtc tcgcccagat atggggggcc aataattggt ggaataagag atttcataac
     5521 aggagctttt attctcacaa gggacgatac tacgctgagc agagaagagt ttgccaacct
     5581 tgcgcttatc gggggttaca agggtaattt gcccgagcct gacataaacc ataatgggac
     5641 actgctttat tctggcaagc aacttttctc tctattcttg ccaagcgact tcaattttat
     5701 aataacatcc aaatggacga aggctacgaa gaaggagggc agagacgtag ttataaaggc
     5761 tggacgacta acaagtgggg taattgacaa ggcctcaata ggggcagaag agccagacag
     5821 tgtactccac aggatagtaa aggattatgg caacgaccga gcaggaaaat tcctagattc
     5881 gatactcgta atgcttaaga cattcataac tcatcgaggt tttacctatg ggtattcaga
     5941 cctatggcta agcgaagaaa ctagaaagga gataaataac gtgattgaaa aggcatacga
     6001 caaagtatat gatttgataa ggcagtacaa cgaggggagc ttgcctctta ctaggggact
     6061 tgccgccgag gaggcattgg aactctatgt tgtcaatgag ttgtcgaggg caagagatag
     6121 agcaggcagg actgccgaca gagcttttcc tgatgataat tctgggataa ttatggcctc
     6181 gaccggtgca cgaggttcaa ctctgaatat tggacaaatg acagctgctc ttggacaaca
     6241 atccattaga ggaaaacgta tacaaaaagg ctaccacaac agatcattac cgcacttcaa
     6301 gagaaatgat gccaacccag attcaaaggg ctttatcaaa tcaaattata gagacggtct
     6361 aagccctctg gaattcttct tccatgcgat gggtggaaga gaaggtcttg tggatactgc
     6421 agtaagaacc cagcagtcag gatatatgca gagacgactc ataaatgcct tggaacactt
     6481 gaaaattgaa tatgaccata ctgtgcgcga cccccacggg aacattattc agtatttgta
     6541 cggtgaggat ggcgttgatc ctgcaaagag tgatcacggg gaagctgtta atatatcacg
     6601 tctaattgag agtgagtcag tagtcgacga aggaaaaaag gctagcgatg tcgaaattga
     6661 aaatatcttg tcgaaacgtg cagacgacct gaaccctaag gtaaagaagc acttggtaag
     6721 cgctttcgca cataataggt tgagcaaaaa tggaattgag aaggtactaa ggaagacgat
     6781 tgacttgata cagcgtgcgc ttgcagaacc tggcgaagcc gttggggttg tcacagcgca
     6841 atctattgga gaacctggaa cgcaaatgac tcttagaact ttccacttcg caggagtcaa
     6901 agaaagaaat gttacgcttg gacttccaag acttatcgaa ttagtggatg cacgcaagaa
     6961 accagttacc ccaacaatgg acatttactt ggataaggaa catagagtct ctagagaaaa
     7021 agcccttgaa gttgcacagc aaataatatt tacgaaggta ggggacttga ttctaaaaac
//
LOCUS       DASQTF010000093         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_851439,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000093 DASQTF010000000
VERSION     DASQTF010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851439"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VE308_02270"
     CDS             complement(<1..349)
                     /locus_tag="VE308_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZA99828.1"
                     /translation="MYANLANANMGLIRSGKVKDIYELDSGNILFHFSDRISAFDVKM
                     ATPVPKKGEVLCKFAEFWFDTLETPHHMLKVVAKDKMEVKKLQMIPLECVVRGYFYGS
                     FVERYKERLGKELP"
     gene            416..1612
                     /gene="purK"
                     /locus_tag="VE308_02275"
     CDS             416..1612
                     /gene="purK"
                     /locus_tag="VE308_02275"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0034028 - 5-(carboxyamino)imidazole
                     ribonucleotide synthase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HZA99829.1"
                     /translation="MVNSDLLAIERPIRVGIIGGGQLGKMIAHEAKRMSLKVIVLDPT
                     EGCPASSIADEQIVADFKDENAIMQLAAKSDVLTYEIELANSAALKTLELRRYPVRPA
                     PETLHIVQNKFRQKSFLRHHKIDVADFEAVRSEDHLYELCENFGFPVMLKACENSYDG
                     RGNFIITSRKQVREAFNIFSRNELMVEKFVPFTKEISIMVARNPSGEVASFPVAENIH
                     KNGILDTSIVPARINPKVELKAKRMAELTMDVLKGAGIFGIEMFVTKEENVLINEIAP
                     RPHNSGHYTNEACSVSQFEQHLRAVLDFPLSKPELLCPAVMVNILGNEHLNGSYAMKG
                     LRQLLSVPGVKLYVYGKMTSRPGRKLGHITATGRTVEEALMRATKARKGFEITAIVTK
                     ETPENE"
     gene            1605..2123
                     /gene="purE"
                     /locus_tag="VE308_02280"
     CDS             1605..2123
                     /gene="purE"
                     /locus_tag="VE308_02280"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZA99830.1"
                     /translation="MNSPLVGIIMGSDSDLPVMNEAKEILDSFGVTNEITIVSAHRTT
                     KRMVHYAITAKKRGVEIIIAGAGGAAHLPGMVASLTSLPVIGVPIKTKSLDGLDSLLS
                     IAQMPPGVPVATMAINGSRNAGILACQILGTKYSHIATRVEEYKRDLESGVLKKAKKL
                     KKSGVKRYMGRA"
     gene            2153..2452
                     /locus_tag="VE308_02285"
     CDS             2153..2452
                     /locus_tag="VE308_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99831.1"
                     /translation="MRFVEHKTTHFTTTSDKEGNDHGDIDPIIVKKLSAHEKLAEEMK
                     LSEIDCEALPLGLQPIERKQYYSHTFSMSPKFVTNKGMVKIRGKNIHFVQLLQRN"
     gene            2456..2851
                     /locus_tag="VE308_02290"
     CDS             2456..2851
                     /locus_tag="VE308_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99832.1"
                     /translation="MPNIDPVPLPYGPLQYYQIHYILQIESNDNDKFLARCRPVTRGY
                     FHNKRIIDVKWTGDDNFAQFLQADAGLTGMLKEVMLKVGEIMIDPQDDHIRIYARWVH
                     EENLAFDPAMLEIADRIALHIKNRVEKND"
     gene            2938..>4398
                     /locus_tag="VE308_02295"
     CDS             2938..>4398
                     /locus_tag="VE308_02295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZA99833.1"
                     /translation="MEKQVSPKSGLKFTDKTVVITGSGTGIGQAIARKFAENGANIAI
                     MGRRKEPLEQTADMLEEIIASSGSSGRLGVFSGVDVADEAGIDSMFESIRKQFGGVDI
                     IVNNAGVSGPVKTFTNASVKEFREAVAIHLTGTYWTSVSGLSAMKRGSKIITIATFFT
                     EENRYEQRPYRFRTPYTAAQGAKNRLAEALAWELVERDIRSIATNPGPVHSDRIYKTV
                     YPKAAAEFLRIGGYPGLSSLDIEKVSAGALPLLGEPADVVAKGVSQVAYDIATSRGQI
                     SSEENVKKLSEIVAGALTKIQEIAEKIQSNTSKMIVDGEFLKQEEVADMVMNLCDDNI
                     SRLINGRVIPNDRVFYPVKPIVGTGVDGLAQLDVKGKVIAITTSSSAKKDSERVKELA
                     RLAHGAGAKDVIVLAHKLQDMSEYSEFHNHTIDMLDEESVHRIFKAAKKRSSKIDSII
                     HFTGDYDYNVPLSSLSRKQWDALVDNFIYIPGLITRE"
BASE COUNT         1378 a          892 c         1041 g         1087 t
ORIGIN      
        1 aaggaagctc ttttcccagg cgttctttgt accgttcgac aaagctgccg taaaagtatc
       61 ctcttaccac acattcaaga ggtatcattt gtaatttttt gacctccatc ttatccttgg
      121 ccactacttt cagcatatgg tgaggagttt caagtgtatc aaaccagaac tctgcaaatt
      181 tgcataatac ctcgcccttt tttgggaccg gtgtcgccat cttgacatca aaagcagaga
      241 tgcgatccga gaaatggaac agaatatttc cgctgtcaag ctcatatatg tcctttacct
      301 ttccgctccg aatcaacccc atgtttgcat tggctagatt tgcatacaat aaaaaattta
      361 tctgtcatta ttgaaaaaca tttaacgtgc tgctaatcat cttatccagt cagatatggt
      421 gaacagcgac cttcttgcca tagaaagacc gatacgcgtt ggcattattg gtggaggtca
      481 acttggaaag atgattgccc atgaagcgaa gcggatgtca cttaaggtta ttgttctgga
      541 tccaacggaa ggatgccctg cttcaagtat agcagacgag cagatagtgg cagatttcaa
      601 agatgaaaac gcgataatgc aattagctgc aaaaagtgat gttctaacct atgagataga
      661 gcttgcaaat tctgcagctt taaagactct tgaactaaga aggtatccag tcaggccggc
      721 accagaaaca cttcacattg tccagaataa gtttagacaa aagtccttcc tgaggcatca
      781 taaaattgat gtcgcagatt ttgaggctgt tagatcggag gatcacctgt acgaattgtg
      841 cgaaaacttt ggctttcctg taatgcttaa ggcgtgtgag aactcgtacg atggacgtgg
      901 aaattttata ataacttcac gtaaacaagt acgcgaggca ttcaacattt tttctagaaa
      961 tgagctgatg gtcgaaaagt ttgtgccatt tacaaaagag atatcgataa tggtcgcccg
     1021 taaccctagc ggagaagtag cgtcatttcc tgtggcagaa aacatccaca agaacggcat
     1081 attagacacc tcaatagtac cagcaaggat caatccaaag gtcgagttaa aggcaaagag
     1141 gatggcagag ttgactatgg atgtattaaa aggggcagga atatttggaa tagagatgtt
     1201 tgttacaaaa gaggagaatg tcttgataaa tgaaattgcc cctcggcccc ataattccgg
     1261 gcactatact aatgaagcct gctcggtgtc gcaatttgag cagcacctga gggctgtgct
     1321 tgactttcca ttgagcaagc ctgaattact ttgtcccgcc gtcatggtta acatacttgg
     1381 aaatgaacat ttgaatgggt cctatgcaat gaaaggtctt agacagctct taagtgttcc
     1441 aggtgttaaa ctttatgtgt acggcaagat gacctctagg ccgggacgca aactgggtca
     1501 tattactgcc acaggcagga cggtagagga agcgcttatg cgggctacaa aggctagaaa
     1561 aggatttgag ataactgcca ttgtaaccaa ggaaacacct gagaatgaat agccctcttg
     1621 ttggtatcat aatgggaagt gattctgatc ttccagtaat gaatgaagca aaggaaattt
     1681 tggattcatt tggcgtgact aacgaaatta caatcgtttc tgctcacagg accacaaaga
     1741 ggatggttca ctatgccatt accgccaaga aaagaggggt cgagataatc atcgctggtg
     1801 caggaggagc agctcatctt ccaggcatgg tggcttcatt aactagtctg cccgtaattg
     1861 gtgttcccat taagacaaaa agcctggatg gccttgattc acttctttca attgctcaga
     1921 tgcctcccgg tgtgcctgtc gcaacgatgg cgataaatgg atctaggaat gcagggatac
     1981 ttgcctgcca aatcctagga acaaaatatt ctcacattgc aacaagagtg gaagaatata
     2041 agcgcgacct agaaagtggc gtgctcaaaa aagcaaaaaa gctaaagaaa tccggtgtca
     2101 aacgctatat gggaagggcc tagagtaaat ttatttgtgg ctgcccaatt gtatgcgctt
     2161 tgtggagcac aaaactaccc attttactac tacttcagac aaagaaggaa atgaccatgg
     2221 agatattgat cccatcatag taaagaaact gtctgcccat gagaagttgg cagaggaaat
     2281 gaagctaagc gagatcgact gcgaagcact tccgcttggc ttgcaaccaa ttgaacgcaa
     2341 gcagtattac agccacacat tctcaatgtc gcccaaattc gtcacaaaca agggcatggt
     2401 gaagatcaga ggcaaaaaca ttcactttgt ccagttattg cagaggaatt aagagatgcc
     2461 gaatatagat ccagttccat tgccgtatgg tcctctgcaa tattatcaga tccactacat
     2521 actgcagata gaaagcaatg acaacgacaa atttttagcg agatgtagac cggtaacaag
     2581 aggatatttc cataacaagc gcataatcga tgtcaagtgg acaggagatg acaattttgc
     2641 gcagttttta caagcagacg ctggtctcac tggcatgcta aaagaagtca tgctgaaagt
     2701 aggcgaaata atgattgacc cccaagatga ccatatcagg atctatgcca ggtgggtaca
     2761 tgaggagaat cttgcattcg atccggctat gctcgaaatt gcagacagga tagccttaca
     2821 cattaagaac agagttgaga aaaacgacta gatctggtcc ctgttttttc aatgggcgtt
     2881 caaaacttct gcgatagttc aagttaactt taataatctt agctcagtgg cagttttgtg
     2941 gaaaagcaag tatctcccaa gtctggctta aaatttacag ataaaaccgt agtgatcaca
     3001 gggagcggaa ctggtatcgg gcaggcaata gccagaaagt ttgcggagaa cggcgccaat
     3061 attgccatca tgggcaggag gaaggaacca cttgagcaga cagcagacat gttagaggaa
     3121 ataattgctt cctcgggttc ttctggcagg ttgggcgttt tttcaggtgt agacgttgcc
     3181 gatgaggccg ggattgattc aatgtttgaa agtataagaa agcaatttgg aggagtcgac
     3241 ataatagtaa acaatgctgg tgtctcaggc ccggtcaaga catttaccaa tgcaagcgta
     3301 aaggagttcc gtgaggccgt agccattcat cttacaggca catactggac atcagtaagc
     3361 ggtctgagcg cgatgaaaag aggctcaaag ataatcacga ttgccacgtt ttttacagaa
     3421 gaaaaccgct atgagcagcg gccatataga tttaggacac cctacacggc cgcacaaggt
     3481 gctaagaacc gcctagcaga agcactagca tgggagcttg tggaacgtga catacgatca
     3541 attgcaacaa acccagggcc tgtccactcc gataggattt acaagacagt atacccaaag
     3601 gctgcggcag agtttctaag gattggaggt tatcccgggc tcagctctct cgacattgag
     3661 aaagttagtg cgggtgctct tccgctattg ggtgaacctg ccgatgtagt agcaaaaggt
     3721 gtttcacaag ttgcctatga catcgctaca tcacgcggtc agatctcatc agaggaaaac
     3781 gtcaaaaaat tgtcagagat tgttgcaggc gctcttacca agatacaaga gatcgccgaa
     3841 aagatacaga gcaatacaag caagatgata gtagatgggg aattcttgaa acaagaagag
     3901 gtagcagaca tggtcatgaa tttgtgcgat gacaatataa gcaggctaat aaacggcaga
     3961 gtcatcccaa atgaccgcgt attctatccc gtcaaaccaa tagttgggac cggagtagat
     4021 ggcctagcac agcttgatgt caaagggaaa gtcatagcta taacgaccag ctcatctgca
     4081 aagaaggaca gtgaaagagt taaagagctt gcaagactcg cacatggggc aggggccaag
     4141 gatgtaatcg tgcttgcaca taagcttcag gacatgagcg aatatagtga atttcacaac
     4201 cataccatcg acatgctaga tgaagaaagt gttcaccgca tatttaaagc tgcgaagaaa
     4261 aggtcaagta agattgattc aatcattcac ttcacaggag actatgacta caatgtacct
     4321 ctgagctcac tttcaaggaa acaatgggat gcattggttg ataatttcat ttacattcca
     4381 ggactgatta caagagaa
//
LOCUS       DASQTF010000094         5335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_756869,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000094 DASQTF010000000
VERSION     DASQTF010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756869"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /gene="thpR"
                     /locus_tag="VE308_02300"
     CDS             <1..563
                     /gene="thpR"
                     /locus_tag="VE308_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZA99834.1"
                     /translation="RLAMRTFVAVDVSTNAIAKVQKEILSTTGWNPRDVKPVEPHNFH
                     FTLIFLGETSDHDVDRIKEMLAGFQFEPFTITYTGIGGFPNPAHARVVWVGVDSEGVQ
                     KLTALANDVIAKMSELGFKADKPFSAHMTFLRAKGRPIRATEISIKYQGRTFGSDPID
                     KVHLKRSELTPSGPIYSNVYTVEAKK"
     gene            560..1975
                     /gene="cca"
                     /locus_tag="VE308_02305"
     CDS             560..1975
                     /gene="cca"
                     /locus_tag="VE308_02305"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR03671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZA99835.1"
                     /translation="MNYTQHMPDPSTLISKVLRLCEPTRSQERRIAEVAQEAKGLVDS
                     YVVRLDDVVDVVFGGSFAKGTWLPDHADIDIFVKIKPSVGIGKFEEMGREIGSEALKN
                     YGPKLRYSDHPYVEVFVHDVRVNIVPCYDVQQGKWQSAADRSPFHTQYISSHFDNEKR
                     RQARLLKKFLKSVGIYGAEISTEGFSGYVSEVLVLKYSSFENILCSAADWQERQIIAV
                     GDYDSDFVKTFNSPLTIIDPVDSRRNLGTAISPQSVAKFMLAARAFLEKPSLEFFKEE
                     EGNKKYHHLSRRSASKKKKKLLLSNVLVVEFSHEKKSPDIIWGQLKRSISAITKQLEL
                     AHFEVLRSSCVTDETSSAALAFLLESITLPVYTKKKGPEIFRRKDTASFVSNREKSKP
                     LAIWVDREMRIAMLIERKATDARKFVKSLLLNHRENSGISKDLIVNKLQIYSGSDRKI
                     IKGLAKEVIGEVVSTERLIFR"
     gene            1935..2732
                     /locus_tag="VE308_02310"
     CDS             1935..2732
                     /locus_tag="VE308_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99836.1"
                     /translation="MVRLSQQNVSFSDEELDIRSPKLVPLITYPHFSEHEYKDRITEM
                     QSLGITSIILSGKTIVNGAHIAGKGCVGIVVKSKAGSSNKICALKIRRTDAARKTMDE
                     EARFHKMANSLGVGPRLEAQTRNLIAMEYVTGQSIIDWVPVVGGIKATKSKMCRVART
                     ILEQCFSLDVAGLDHGELSRLARHVIVSDNSSSYIIDFESASTTRRTSNVTAAAQSIF
                     LYGIVADKVKKILGDIDREKVVHALRTYKHSHTRANFDAVLDSLLIK"
     gene            complement(3425..4183)
                     /locus_tag="VE308_02315"
     CDS             complement(3425..4183)
                     /locus_tag="VE308_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99837.1"
                     /translation="MPSLVSTKAFAQQDIASEVNERVNEVLTQVNPLNSEDEDQQQDA
                     SDSDTATAADEQQVEDEEATTTAADEQDASDSEASSTPENRDSQINLDSNSQGETSRV
                     EEDERVTVDPIIQTEVQTGINVNADTALVMDEEDCEDANDEVFQGNGQDSNQQARSEG
                     RVGDNGIYVSPKVQTSEQIAYNVNVDVDLVLVEGCDPSDEVVQANDQSDTQVISSGLE
                     ASKDGSTTIIPAEQRSEQRSGNIGVNKDVILPVL"
     gene            complement(4793..>5335)
                     /locus_tag="VE308_02320"
     CDS             complement(4793..>5335)
                     /locus_tag="VE308_02320"
                     /inference="COORDINATES: protein motif:HMM:NF042555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HZA99838.1"
                     /translation="ILFLILAVVNMKAEQFSKNVLALDPRIRFAGVMEKSGHLYAGTQ
                     REGVEEHLKGRNSEISYAQSAYIVDLRRMFTPELGKLKTVAYMYDKVNLINFPVRDHL
                     LVISAEAEAKVDDLYEKILRYVTSVENELSLYPPGNIVNHEKKEVLRNLHESGIPEDM
                     IADQLDLDVNTVKMIIAEIK"
BASE COUNT         1581 a         1120 c         1144 g         1490 t
ORIGIN      
        1 caagattggc gatgcgtact tttgtcgctg tggacgtgtc aacaaacgca atagctaaag
       61 tgcaaaagga aatactgtct acaacaggat ggaatcctag agatgtcaag cctgtcgagc
      121 cgcacaattt tcatttcaca ctaatttttc ttggggaaac aagtgatcat gatgttgata
      181 ggattaaaga aatgttggca ggatttcaat ttgaaccatt tacaatcacc tacaccggaa
      241 ttggcggctt tcccaacccg gcacatgccc gggtagtctg ggtaggtgtc gactcagagg
      301 gcgttcaaaa attaactgcc cttgcaaatg atgttatagc aaagatgtcc gagcttggct
      361 ttaaggcgga caagcctttt tcagcccata tgacattcct tcgggccaag ggcagaccga
      421 tcagggcaac tgaaatttct ataaaatatc aggggagaac gtttggatct gacccgatag
      481 ataaggtgca cctcaagaga agtgagctca cgccatcagg tcccatatac tctaatgttt
      541 atactgtaga agctaaaaaa tgaattacac acagcatatg ccagatccta gcaccttaat
      601 ctccaaagta cttcgtcttt gtgaacccac tcgcagccag gagcgcagga tcgctgaagt
      661 agcacaagag gcaaagggac tcgtagatag ctacgttgta cggctagatg acgtcgttga
      721 tgtagtattt ggcggctctt ttgccaaggg cacttggctt ccagatcacg ctgacattga
      781 cattttcgtc aagatcaagc catcggttgg cattgggaaa ttcgaggaga tgggcagaga
      841 gatcggcagc gaggcactca aaaactacgg gcccaagctt cgatattcag atcatcctta
      901 tgtcgaagta tttgtacatg atgtcagagt taatatcgtt ccctgctatg atgtccagca
      961 gggcaagtgg cagagcgcag cggatagatc acctttccat actcaataca tcagcagtca
     1021 ctttgataac gaaaaaagga ggcaggctag acttttgaaa aagttcttaa aatcagtagg
     1081 catttatggc gcagagattt caactgaagg gtttagcggc tatgtttcag aggttttggt
     1141 gctcaagtac tcctcatttg agaacatctt gtgctcagct gctgactggc aggagaggca
     1201 gataatagcg gttggcgatt atgattcaga ttttgtcaag acattcaaca gcccactgac
     1261 aattattgac ccagtggaca gtaggagaaa ccttggtact gccatttcgc cacaaagtgt
     1321 ggccaagttc atgctagctg ctagggcatt tcttgagaag ccatcacttg agttcttcaa
     1381 agaagaagaa ggcaataaaa aatatcatca tctcagtcgt cgtagcgcaa gcaagaagaa
     1441 gaagaagttg cttctttcaa atgtgcttgt agtagaattt tcacatgaaa agaaaagtcc
     1501 agatattatc tggggtcagc tgaaacgaag cataagcgca ataacaaaac aattggaact
     1561 ggcacatttt gaggtactaa ggagctcctg tgtcacagac gaaacaagct cagctgccct
     1621 tgcgttcctg cttgaatcaa taaccttgcc ggtatacacc aaaaagaaag ggcctgaaat
     1681 tttcagaagg aaggacacag ctagctttgt ttcaaacagg gaaaaaagca aaccacttgc
     1741 aatctgggtt gacagggaaa tgagaatcgc aatgcttatt gagcgcaagg ctacggatgc
     1801 aagaaagttc gtcaaatcac ttctgctcaa tcatcgtgaa aacagtggta tttcaaaaga
     1861 tctaattgta aacaagctcc aaatttatag cggaagcgat aggaagataa taaaagggct
     1921 ggcaaaggag gtcattggtg aggttgtctc aacagaacgt ctcattttca gatgaggagc
     1981 tagacataag atcacccaag ctcgtgcctc ttataacata tcctcacttt tcagaacacg
     2041 aatacaaaga tcgaattacc gaaatgcagt cacttggaat aacctcaatc atattgagcg
     2101 gaaaaacaat agtaaatggc gctcatattg ccggcaaggg ctgcgtagga attgtagtca
     2161 agtcaaaagc agggagcagc aacaaaattt gtgcgctcaa gataagaagg acagacgcag
     2221 ccagaaagac gatggatgaa gaagcacgtt ttcacaaaat ggcaaacagc ctaggagtag
     2281 gcccgcgtct tgaagcgcaa actaggaatc ttattgcgat ggagtatgtc accggtcaga
     2341 gcatcattga ctgggtacca gtagtaggag gcatcaaagc tacaaagagc aaaatgtgca
     2401 gagtagctag gactattctt gagcaatgct tcagccttga cgtagcgggc cttgaccatg
     2461 gtgagctcag caggcttgca cgacacgtca ttgtttctga taactcctcc tcctacataa
     2521 tcgactttga aagtgcaagt actacaagaa gaacatccaa tgttactgcc gctgcacagt
     2581 cgatattcct ctatggcata gttgcagata aagtaaaaaa gatcttgggc gatatcgaca
     2641 gagaaaaagt agtccatgca ctgagaactt acaagcattc acacacaaga gctaattttg
     2701 acgcagtgct cgactcgcta ctaataaaat agttttaatt cacaatggtc atataagaga
     2761 cacatacaca catacacaca ctctctctat gtgtgtgttt agaaacagta tttttaggga
     2821 gagatataga gattgcgaga gtagaggtct ttgaacagtt ttttagctga tgcagacagg
     2881 cgccttgcaa agataatgag attagttggc aaatacaaaa ttgactatag agatgtatat
     2941 gtatagatca gatcagagct cttgtcaaat aggctcgcct ctatccttca agagggagaa
     3001 caacatggcg cagtcatttg gtattttttc gtcttctaat attatagata gcggcaacaa
     3061 ggccaagaac cctatgatag ctaacaagaa taatagcctc acttgctgac aagcaagact
     3121 atttcctttt cttggccatc gctgccgcaa gtaggtcttt tgtgttcttt agtaatacat
     3181 gtttgtcccg cttattctat tacattatca gttgacattg tcctgatagg cagagacatt
     3241 gcaattatca aattaataac tgttcgatat aaaaatgata gaaaagagag aggtatatac
     3301 atatgattca agatattgtt aataaaaaat tttacttcgg tgacggatgt gtgagttagt
     3361 gaaagatacc tctatacaca cacacaccac gcaccgtgta ttcaggacta gccaggttat
     3421 tcagttacag cacaggcaaa attacgtctt tgtttacgcc aatatttccg gatctctgtt
     3481 ctgacctttg ctctgctgga ataatggtcg tgctgccatc tttgcttgcc tcgagaccgc
     3541 tactgattac ttgagtgtca ctttggtcat ttgcctgaac tacctcatcg cttggatcgc
     3601 atccctctac aagcactaaa tcgacgtcca cattaacatt gtaggcaatc tgttctgaag
     3661 tttgtacttt tggactaaca taaataccgt tatcgcctac cctgccttca ctacgtgctt
     3721 gttggtttga atcttgtcca tttccttgaa atacttcgtc gtttgcatct tcgcagtcct
     3781 cttcatccat taccaaggct gtgtctgcat taacatttat gccagtctgt acctcagttt
     3841 ggattattgg atcaacagtc acacgttcgt cttcctcaac gcggctagtc tctccttggc
     3901 tattagaatc cagatttatc tgagaatctc gattttctgg tgttgatgat gcttcactgt
     3961 cagatgcgtc ctgttcatct gctgctgttg ttgttgcttc ttcgtcttcg acttgttgtt
     4021 catctgctgc tgttgctgta tcactgtcag atgcgtcttg ttgctggtct tcatcctcag
     4081 aatttagagg atttacctgt gttaatactt cattgacgcg ttcattgact tcagatgcaa
     4141 tgtcttgctg cgcaaatgct tttgttgaaa ccaatgatgg cattgatgat gccgttagca
     4201 tcatagctga caacattgtt gctatcaaga gtacccgtgt atttgataag atgtgattat
     4261 tcatacttgg ataagcctta ttaatcaata gcttataaca acatcgccaa atttccctgg
     4321 aataagtatt gagaatatta tataagatct tatttatgat aattgttcta tccttctata
     4381 ctttgataga atgtgctccc acaggccacc acaacatgag tggcagatgc aataatagaa
     4441 atgtgcaatg gaagcaaccg tagggaaaag ggggggagga tacatctaat attctctaaa
     4501 aaaaacagcc gagggatgta tatagaatta agggtatctc aaatgacaac cgaatactag
     4561 agacacagga caagagcatc atctcgtcga tatatacacc cattatcaga atcccaagaa
     4621 tgctgtttcc gtgtttctga tggcagttaa tccttgcgga ccggccgtat atatgcatga
     4681 cattgcgaca cgcgcacgca cacgtctcct gcaccctcta ctatcaatat agctatatgg
     4741 tatctatcaa aaaaaaatca gttggctagt tcaaatggag aggacaagtc gtttacttga
     4801 tctccgcgat gatcatcttc actgtgttga catcaagatc aagctgatct gctatcatgt
     4861 cttcgggaat cccagattca tgaagattac gcagtacctc tttcttttca tgattcacaa
     4921 tattgccagg tggatacagt gagagttcat tttctacaga tgtaacatat ctcaatatct
     4981 tttcatatag gtcatcgacc tttgcttcag cctctgcaga tatcacaagc aagtgatccc
     5041 tgactggaaa attgattagg ttaaccttgt cgtacatgta tgcaactgtc ttgagcttgc
     5101 caagctctgg tgtgaacatc cttctcaggt cgacaatata tgcagactgg gcgtaactga
     5161 tctcagaatt tctgcccttc aaatgctctt caaccccttc cctctgggtt cctgcataca
     5221 ggtggccgga tttttccatt acccctgcaa atcttatcct aggatcgagt gctaatacat
     5281 tctttgaaaa ctgttccgct ttcatattta caaccgctaa aataagaaag agaat
//
LOCUS       DASQTF010000095         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_22167,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000095 DASQTF010000000
VERSION     DASQTF010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22167"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VE308_02325"
     CDS             <1..1341
                     /locus_tag="VE308_02325"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZA99839.1"
                     /translation="EDRRVGLSYLEKSSRYVAFNQKVGGYYRYAREESIMSSSHADRY
                     IEACDHSFNTYSKSIQPLQAFLKEKEPIERFSFFDSMSQKEIPFNNLIAGEDIEAARR
                     IYSMTIKAKALDILRGLLPASTMTNIGITGNGRAFEYLLTLMYGSKLKEIRSIAAQLF
                     NELNTLIPSFIRRANDKYGQALQEYTAKTGNAIGEFARSHLSGIPPEEEPELVRLIDY
                     KDNFQAEVNVISAILYEQAQGQSLHRITNYVKSMPIEERSKVLRAYTEFRTSRRHRPG
                     RAFEMVDYTFELFTNFGIFRDLHRHRILTIERQLLSTRHGYDLPQELVDSGLDKDFRD
                     CMSLSKQVYEEIAKTMPEEAQYVVNFAYRYPYFVKMNLREACHMIELRTAPQGHPDYR
                     IVCQKIYSEIKQVHPQLAHGIKFIDMNRYQLERFDAEKKSEKKRQQVTRTISGF"
     gene            1420..1914
                     /locus_tag="VE308_02330"
     CDS             1420..1914
                     /locus_tag="VE308_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99840.1"
                     /translation="MVDKTIPTKDERAVDVYDTMRDNLIRTVDEFAKAQPQYSQSISN
                     LQLDYIQTTKNMIQSAFSAQKQLANTLNVPSVPYGEVFTRQSNEVTNNAIRSVGINNQ
                     LTINALDAARENLKIYNRTVDTLTDFSTNITKAWTTFYTQQQQQFAQQQQQFAQQQQQ
                     FTRQ"
     gene            1982..>2428
                     /locus_tag="VE308_02335"
     CDS             1982..>2428
                     /locus_tag="VE308_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99841.1"
                     /translation="MSTTVTEYVCQDCGTLLRHSNPTTLESMRDVHNQLCIVKRQKTM
                     AAQVAAPKPAAAASAAAAKTPAATATSPTAAIPAAAAAPPPAAGGGGGSGGGPTTISL
                     TGTGTSYSKAEGPIDPKFQEKRQVTGTYQGIKVWGPIDPPGQLGIWG"
BASE COUNT          793 a          546 c          533 g          556 t
ORIGIN      
        1 gaggatcgta gagttggcct ctcatatcta gaaaaatcgt ctcgttatgt agctttcaac
       61 caaaaggtcg gcggatatta tcgatatgct agagaagaga gtattatgtc gtcatctcat
      121 gcagacagat acattgaggc ttgcgaccac tcctttaaca cttacagtaa gagtatccag
      181 ccattacaag ccttcctaaa ggaaaaagag ccaatagagc gctttagttt ttttgattcg
      241 atgtcgcaga aagagatacc ctttaacaac ttgatagcag gcgaggacat tgaagccgct
      301 cggcggatct acagcatgac gataaaggca aaggcactag atattctccg cggtctcttg
      361 cctgcatcca ctatgacaaa tatcggcatt acaggcaacg gcagagcatt tgaatacttg
      421 ctaaccctaa tgtatgggtc aaagctaaaa gagatcagat ctattgcagc tcagttattt
      481 aatgagctaa acacactcat cccttccttt atcaggcggg caaatgacaa gtacgggcag
      541 gcactgcaag aatatacagc aaaaacggga aatgcaattg gcgagtttgc caggtctcat
      601 ctatcgggaa tcccacctga agaagaaccc gaacttgtca ggctgattga ctacaaagac
      661 aactttcaag cagaagtaaa tgtaatatct gcaattctgt atgagcaggc tcagggccag
      721 tcgcttcaca ggattacgaa ttatgtcaaa tcaatgccga ttgaagagcg gagcaaagtg
      781 ctccgcgcat acacagaatt tcgtaccagt cgccgacata ggccaggcag ggcttttgaa
      841 atggtcgatt atacttttga attgttcact aattttggaa tattccgaga tctgcatagg
      901 cacagaattc ttacgattga gcggcaattg ctctcgacaa ggcacggtta tgatcttcct
      961 caagagctgg ttgattcagg acttgacaag gactttagag attgcatgtc cctatcaaaa
     1021 caggtttacg aagaaatagc caagactatg ccggaggaag ctcagtacgt cgtcaacttt
     1081 gcatatcgct atccctattt tgtcaagatg aatctgcgag aagcatgcca tatgatagag
     1141 ttgcgtacgg cgccacaggg tcatccggac taccgaattg tatgccaaaa gatatacagt
     1201 gaaatcaagc aggtacatcc ccaacttgcg cacggaatta agttcattga tatgaataga
     1261 tatcagctgg aaaggtttga tgcagaaaaa aaaagcgaga aaaagaggca gcaggttact
     1321 aggacaatat ctggttttta gtaccattac atggtaatca acaccaatat tgaccaagct
     1381 ttatatatta ccattgatta ccattatatg gtaataaaaa tggtcgacaa aactatccca
     1441 acaaaagacg aaagagcagt agatgtatat gacacaatga gagacaacct tatcagaaca
     1501 gtagatgaat ttgcaaaggc tcagccacag tattcacagt caatttctaa ccttcagctg
     1561 gactatatcc agacaactaa gaacatgatt cagagtgcgt tttcggctca gaagcagtta
     1621 gcaaacacct taaacgtccc gtcagtgcca tatggagaag tgttcacaag gcaatcaaac
     1681 gaagtgacaa ataacgcaat caggtcggtg ggcataaaca atcagctgac gatcaacgcg
     1741 ctggatgcag caagagaaaa tctaaagatc tacaacagaa cagttgatac actgactgac
     1801 ttttccacca acatcacaaa agcgtggaca acgttctata cgcaacagca gcaacagttc
     1861 gcacaacagc agcaacagtt cgcacaacag cagcaacagt tcacaaggca gtaaaaaact
     1921 gccttttttt catcttcctt tttggcatag ttaataaata agttaaaatg catactatct
     1981 tgtgtctacc acggtaaccg agtacgtatg ccaagactgt ggaacccttt tacgtcacag
     2041 caatccgacg acccttgaaa gcatgaggga tgttcacaat caattatgta ttgttaaaag
     2101 gcaaaagaca atggctgcgc aggtagctgc gcccaagcct gcagccgctg cctcggctgc
     2161 tgcggccaaa acaccagcag caactgcaac ctctccaaca gccgctattc cagcagcagc
     2221 agcagcacca ccaccagcgg caggaggagg aggtggtagt ggtggtggcc caaccacgat
     2281 atcactcact ggcacgggaa caagctacag caaagctgaa ggtccaattg accccaagtt
     2341 ccaagaaaaa agacaggtaa ctggcaccta tcaaggaatc aaagtatggg ggccaattga
     2401 cccaccagga caattaggca tatggggt
//
LOCUS       DASQTF010000096         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_481659,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000096 DASQTF010000000
VERSION     DASQTF010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481659"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(459..>2680)
                     /locus_tag="VE308_02340"
     CDS             complement(459..>2680)
                     /locus_tag="VE308_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HZA99842.1"
                     /translation="RQANFMSQNNNIVTLKVLEAYTRDVGRGTARIDYDTMGSLGVST
                     GDIIEIKGKRRTVVKCLPLYPSDEGKGIVRLDGLVRNNAGIGIGDAINARKVKAVPAE
                     KVIISPLEAIPPIDERYLADALESVPLIKGDNVMVPYFGGRLTFQIIGITPPPAVDTA
                     AIVTNKTTFTITEKDEALRGMPQVSYEDIGGLRDEMQKVREMIELPLRHPEIFEKLGI
                     EAPKGVLLFGPPGTGKTLLAKAVASESNSHFISISGPEIMSKFYGESEARLREIFKEA
                     REKAPTIIFIDEIDSIAPKREEVMGEVERRVVSQMLSLMDGLEGRGKVIVIAATNRQN
                     ALDPALRRPGRFDREIEIKVPDKNGRLEILQIHSRNMPLDTDVDQKKIAAVTHGFVGA
                     DLEYLCKEAAMKCLRRLLPDLNLEDEKLPPETLDRLIITQNDFNQAIKDVMPSAMREV
                     FLESPDVKWDDIGGLEGVKRELQEAVEWPLRYPDLYAKIGHTVPKGILLHGPSGTGKT
                     MLAKAVATESEANFISVKGPELLSKWVGESERGIREIFRRARQAAPCVIFFDEIDSIA
                     MARGGGMGGEMGGGSIGTNDRVISQILTELDGISELHGVVVLAATNRPDMIDTALLRP
                     GRFDRIVFVPNPDRRTRKRILEIYVTDKPIGTDVDLEKIADITDGFSGADATAIVNTA
                     VSLVLHEYLARYPTPEEAAKHASEAHVMMRHFEEAVKKIRTQRERKPEETRALSYYR"
BASE COUNT          645 a          669 c          511 g          855 t
ORIGIN      
        1 cgcatgatga gctgaaggat tggctacaca gtggccaagt agatgtgctc agaatgaaca
       61 aatagaatat tgaatctatt gtgtgaggcc gattcgtggg cattatgtac ggcctttact
      121 tttttctatt ctgtgaaaat actgaaaaac ggcaaatgtt ttgataacaa acacagataa
      181 tcgaagaaca actcgaaagc cacccaatgc actgagaatg tatataaggg ctatgatgcg
      241 tacttgaact tttttacatt gcaacgcatc ttatgacgtc aaggtgccat atgcatcgtg
      301 ctattttgga tgcggagagc agctgaaaag ctatgaattt ttcaatcgag tagagtttat
      361 acggatttcg cgcatctcca gggaatactg tgatgtggcc gtacatatga ggaagagtga
      421 ggcagcaaaa gcagcaatac ctcttttttc gtccgctact accgataata tgacagcgct
      481 ctagtttctt ccggcttcct ctctctctga gtccttatct tcttgactgc ctcttcaaag
      541 tgacgcatca tgacatgtgc ttctgatgca tgctttgctg cttcttctgg tgtcgggtat
      601 ctcgccagat actcatgtag cacaagggat acagcagtat ttacaatggc tgtagcgtca
      661 gctccactaa aaccatccgt tatatctgct attttctcca aatcaacatc tgttcctatc
      721 ggcttatctg taacataaat ttcaagtatt ctctttcttg ttctcctgtc agggtttggt
      781 acgaatacta ttctatcaaa tcttccaggc ctcaataagg cggtatcaat catatctggt
      841 ctattggtag ctgcaagcac aactactccg tgtaactcgc taattccatc tagctcagtc
      901 aagatttgcg atattaccct atcatttgtt cctatactgc caccacccat ttcgcctccc
      961 attcctcctc ctcttgccat cgcaatcgaa tcgatctcgt caaagaagat aacacatgga
     1021 gctgcctgcc ttgctctccg aaagatctca cgaattccgc gttcggattc gccaacccac
     1081 ttgctcaaaa gttcaggtcc ttttactgaa atgaaattag cttcagattc tgttgccaca
     1141 gcctttgcca gcatagtttt tcccgttcct gacggtccgt gcagcaagat accttttgga
     1201 actgtgtgac ctatctttgc atagaggtca gggtatctga gcggccattc gactgcttct
     1261 tgtaattctc tttttactcc ttcaagccca ccaatatcat cccattttac gtcaggagat
     1321 tccagaaata cctctctcat ggctgaaggc atgacatcct ttattgcttg attaaaatca
     1381 ttttgcgtta taatcagcct gtctagcgtt tctggcggca atttctcatc ttctagattg
     1441 aggtctggca acaatcttcg aaggcacttc atggctgctt ctttacaaag atattccaag
     1501 tctgctccga caaaaccatg tgtaactgct gctattttct tctggtctac atccgtgtca
     1561 aggggcatgt tacggctatg aatctgaaga atttccaatc gtccattctt gtctggtact
     1621 ttaatttcaa tttctctgtc gaatctccca ggtcttctca aagcagggtc aagtgcattt
     1681 tgcctattgg ttgcagcaat aacaatgact ttgcctctgc cttcaagccc atccatcagt
     1741 gacaacattt gcgaaacaac tcttctctcg acttctccca ttacctcctc tctctttgga
     1801 gcaatagagt cgatctcgtc gataaaaata atggttggag ccttttctct tgcctctttg
     1861 aatatctctc tcaatcgcgc ctctgactct ccatagaatt tgctcatgat ttcaggtcct
     1921 gatatgctga taaagtgaga attgctctca cttgcaaccg ccttagctaa caatgtctta
     1981 cctgtgcccg gtggaccgaa aaggagtaca cctttggggg cctctattcc aagtttttca
     2041 aatatttctg ggtgtcttag tggcaactct atcatttccc tgaccttttg catttcgtcc
     2101 cttaggccgc ctatatcctc gtaagaaacc tgaggcatcc ctcgtaatgc ttcatccttt
     2161 tcagttatgg tgaatgtggt cttgttggta actattgcag cagtatcaac agcaggaggc
     2221 ggtgtaatcc ctattatctg aaatgttagc cttccaccaa agtatggaac catcacgtta
     2281 tcacccttga tcaggggcac gctttctaga gcatctgcga ggtagcgctc atcaattggc
     2341 ggtatcgcct cgagtggaga tattatcacc ttctccgccg ggacagcctt gaccttcctt
     2401 gcatttatcg catctcctat gcctatgccg gcattatttc ttaccaatcc atctagtctg
     2461 acgattccct ttccctcatc agatgggtag agtggtaagc atttgactac tgtccttcgt
     2521 ttgcccttta tttctataat atcacctgta cttactccca aagaacccat ggtatcgtag
     2581 tcaattcttg ccgtgcccct accgacatcg cgggtatatg cttcaagaac ctttagtgtt
     2641 acaatgttat tattctggct cataaaattt gcttgcctgc
//
LOCUS       DASQTF010000097         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_481762,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000097 DASQTF010000000
VERSION     DASQTF010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481762"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VE308_02345"
     CDS             <1..542
                     /locus_tag="VE308_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1297 domain-containing protein"
                     /protein_id="HZA99843.1"
                     /translation="KIEQGLVTKEDLDKATVEQYVPGTYFNLNFFVSPLTGETEFLGI
                     ERRLQTNIHDIVSLSARQQMEIDVQIQNIEVGHTPASIRESLLEKVFEMGDRIAVAVR
                     KEYPPGVIGPISLQSVVTVDLDFVVYDVSLRVPGNPIMATTSPYTKYYYGHTMGVGRR
                     IAMEIKDAIGQQKLPDIVT"
     gene            complement(544..975)
                     /locus_tag="VE308_02350"
     CDS             complement(544..975)
                     /locus_tag="VE308_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HZA99844.1"
                     /translation="MSGDPSQDDEEYIFAKISYVGFVDQLGLEGVVVLKSNDGKEFPM
                     RAFSGEVARHISRFQEGDKGSIPTIYNLVEEIAVMQDLLLVKVHVYQSGSVLRANLYF
                     KSKKDEIVLRNYRASDSIALAAYYDIPIKVRKGLFEEAIER"
     gene            1078..1935
                     /locus_tag="VE308_02355"
     CDS             1078..1935
                     /locus_tag="VE308_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99845.1"
                     /translation="MEEKKWQALRESGFRVFFVGFFSLVIAFLIYSRLTTPSSELLRV
                     ESQDLMGLAWGMLAITGASMASMVYGAYVIFRSEHIRNALGPTTNSLISFITGAFSDR
                     NYWKIMIIAAIGYGIFFGFLSQILVYRADVSFNEKGIKVPSLDLIPCCAAPGYMPIAT
                     VYITDHFLILIIPLNVILATIVSLLVGFNVALSIYAFRLKKSLQTKTSLAAGIGATSG
                     LFVGCPTCAGSLISALLGIGIAGAGGTSASVLAPFQTLFIAASVPALVVAPFLIGRGI
                     RSASSCNIT"
     gene            complement(1928..>3385)
                     /locus_tag="VE308_02360"
     CDS             complement(1928..>3385)
                     /locus_tag="VE308_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HZA99846.1"
                     /translation="GAQPFSSCDGRILLEHNGEIYNYKKIRERLGKRHKFTTMTDSEV
                     IVHLIEDHLRKNSLLGAIKKTVAELDGVYALAIQDKKTGETVLVRDRIGVRQLYYSDT
                     SRFVAFASERKALWKIGIKEPTRGILPGSAIIISQDGRLQSFQVADPIPQKVRIIHRT
                     MASAVEAYEKALVDAMEKRVQDFQRIGIIFSGGIDSVLIAYLAAKMVPEVICYTGGVT
                     GSSDIVYARQIADRLGLNLKVCELDQEGVERLIPEVMNVIEDSNAGQVEVALPVYCAV
                     KLAHEDGIKVMLTGQGADELFGGYSWYAKVVEKEGYKMLRRHMIEDLLLLYKETLERE
                     DKITMAHSIELREPFLDPEVIKVALATSLRLNVRGGADSLGKHVHRKLAEALGIPEDI
                     AYRIKEAAQHGSGMHGMLDDIARKHGFDDSTVPDIYLEELKMREKIGSSQRYGYLFGD
                     GKIWIAEPHMQMYLDSILKKMAGLELIAQYKNRVK"
BASE COUNT          832 a          840 c          725 g          988 t
ORIGIN      
        1 aaaagattga gcagggactt gtgacaaagg aggatctcga caaggcaaca gtcgagcaat
       61 acgtgccggg cacttatttt aatttgaatt tctttgtctc tcctcttaca ggtgagacag
      121 agtttcttgg aatagagcgg aggcttcaga ctaacataca cgacattgta tcactttctg
      181 caagacagca gatggaaata gatgtccaga tacaaaatat cgaagtaggc catactccag
      241 cgagcataag ggagtcactt ctagagaaag tctttgaaat gggtgacagg attgctgtcg
      301 cagtaagaaa ggagtatccg ccaggcgtta tcggccctat ttcgcttcaa agtgttgtga
      361 cagttgacct tgactttgta gtatacgatg tttcattgcg cgtgccgggt aatcctataa
      421 tggcgactac aagcccctat acaaaatatt attatggaca tacgatggga gtaggccgca
      481 ggattgccat ggagataaag gatgctattg ggcagcagaa gctgccagac attgtgactt
      541 aacctagcgc tctatagctt cttcaaaaag gccctttctt accttgattg gtatgtcata
      601 gtacgccgcc agtgctatag aatctgaagc gcgataattg cgaaggacaa tctcgtcctt
      661 tttgctctta aagtaaagat ttgcccgcag aactgagcca ctttggtaga catgcacttt
      721 gaccaacaga agatcctgca ttacggcaat ttcttcaacc aagttgtaga tcgtaggtat
      781 actgcccttg tctccttctt gaaaacgtga aatgtgcctt gccacctcac cggaaaacgc
      841 tcgcattggg aactccttgc cgtcatttga cttcaatact acaacccctt caagccccaa
      901 ctggtctacg aacccgacat aacttatttt ggcgaaaatg tattcctcgt catcttgcga
      961 agggtcccct gacattgtgt cgctactata atagacttac cgagaattaa gggtttacga
     1021 atctacgata ggatttaatt tgcaatcgta gctgactcca tatacagtta atcagacatg
     1081 gaagagaaga aatggcaagc tttaagggag agcggtttta gggtcttttt tgtaggtttc
     1141 ttttcgcttg taattgcatt cctaatatac agccggctca cgacgccttc ttcagagcta
     1201 ttgagggttg aatcgcagga tctgatgggg cttgcatggg gcatgcttgc cataaccggc
     1261 gcttccatgg catcgatggt atatggagca tacgtcattt ttcggtcaga gcacatacgg
     1321 aatgcactag gccccaccac caatagcctg atttcattta tcacaggtgc attttctgac
     1381 cgaaactatt ggaaaataat gataattgct gcaattggct atgggatctt tttcggcttt
     1441 ctatcgcaga tacttgtata ccgggcggat gtctctttca atgaaaaggg gatcaaggtg
     1501 ccatcgcttg acttaatacc gtgctgcgct gccccaggct acatgcccat cgctacagtc
     1561 tacatcactg atcatttttt gatattgata ataccactca atgtgattct tgctactatt
     1621 gtatctttgc tggtcggctt taacgttgca ctgagcatct atgcttttag attgaaaaag
     1681 tcacttcaga caaagacatc ccttgccgca ggcattggag ccacaagcgg cctgtttgtc
     1741 ggatgcccca cctgtgctgg cagtctcatc tcagccctgc ttggaattgg gatcgctgga
     1801 gcaggaggca ctagcgcaag cgtgcttgcc ccatttcaga cactctttat agcggcgagc
     1861 gtccctgcgt tggtcgtcgc accgttcctg attgggcgag gcatcaggtc ggcttcaagc
     1921 tgcaacatta cttgactcta ttcttgtatt gcgctataag ctccaacccg gccatctttt
     1981 ttaatatgct atcgaggtac atttgcatgt ggggttctgc aatccatatc ttcccgtcac
     2041 caaagaggta gccatacctc tgtgagcttc ctattttttc acgcatcttg agctcctcaa
     2101 ggtaaatatc cggtactgtt gaatcgtcaa atccgtgttt ccttgctata tcatctaaca
     2161 tcccatgcat acctgagcca tgctgcgccg cttccttaat cctgtaggcg atgtcctctg
     2221 gtatcccaag ggcctctgca agctttctat gtacgtgttt gccaagacta tcagctcctc
     2281 ctctaacatt gagccgtaag ctggttgcta gcgcgacctt gataacttca ggatcgagaa
     2341 atggttccct aagctcgatg ctgtgcgcca ttgttatctt gtcttcacgc tcaagtgttt
     2401 ccttgtagag caaaagaaga tcctctatca tatgcctgcg cagcattttg taaccttcct
     2461 tttctacgac cttagcgtac catgaatagc ctccaaataa ctcatctgcg ccctgacctg
     2521 taagcatcac ttttattccg tcttcatgag ccagcttgac agcacagtat actggcagcg
     2581 ccacttcaac ctgtcctgca ttactatcct cgatgacatt cattacttca ggtatcagcc
     2641 tctccactcc ctcttggtca agctcgcaaa ccttcaggtt aaggcctagt ctatcagcta
     2701 tctgtcgggc atagacaatg tcgctagatc cggttacgcc accggtgtag catattactt
     2761 caggcaccat cttggcagca aggtacgcaa taagcacact atcgatgccg ccagaaaata
     2821 ttatccctat cctctgaaag tcttgaacgc gcttttccat tgcatcaacc aatgccttct
     2881 catatgcttc aactgcagac gccattgttc tatgtattat tcggaccttt tggggaatcg
     2941 ggtctgcaac ttggaagctc tgaagccggc cgtcctgcga aataatgatt gcactacctg
     3001 gcagtatacc tcttgttggc tctttaatcc cgatcttcca caaggctttg cgttcagatg
     3061 caaaagctac gaatctgctc gtatcagaat aatacagctg ccttacacct atcctatccc
     3121 ttacaaggac tgtctcgcct gtcttcttat cctgtattgc aagggcatag acgccgtcaa
     3181 gttctgcaac cgttttcttt atggctccaa gcaggctatt tttacgtaga tgatcctcga
     3241 taagatgcac aattacttca ctatcagtca ttgtagtgaa cttgtgacgt tttccaagcc
     3301 gttctcgaat ttttttgtag ttgtatatct cgccattgtg ttctagcaaa atccgcccgt
     3361 cgcagctact aaaaggctgt gcgcc
//
LOCUS       DASQTF010000098         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1320099,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000098 DASQTF010000000
VERSION     DASQTF010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320099"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            750..1697
                     /locus_tag="VE308_02365"
     CDS             750..1697
                     /locus_tag="VE308_02365"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HZA99847.1"
                     /translation="MYALSTPPPSSLFAQQQQQQQTTSNYSFVTAWGSSGRAFGQFSQ
                     PFDVATDSAGNVYVTDSTALSNQVQKFTSDGTFILSWGSLGTGAGGFANPAAITIDSN
                     GIAYVTDWGNAEQAVQKFTSDGSFLSMWGSTGLGEGRFITPGGVAVDSQGFVYVGDFG
                     ENNNIQKFDSDGNFILSWGSPGSNDGQFTNPAAIAIDSQGVIYVVDNGNDRVQKFDTN
                     GNFLGKWGTSGSGDGQFNSPAGIAIDSSDNIFIVDRANDRVQVFDPNGNFITKWGSRG
                     SADGQFNNPIGVAIGTDGRVYVTDEGNARIQVFSRGGGG"
     gene            1714..2466
                     /locus_tag="VE308_02370"
     CDS             1714..2466
                     /locus_tag="VE308_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99848.1"
                     /translation="MIQRTFLLFISNIHIQFGTVPIGMRKSFLRSVFIAILFSALVIV
                     PISLGVTPVYAQGPPIAPDNNQGLPPGPPPRFPLPFDQQSPFDQASNFSFGPIASIQN
                     NESGQPAWVVLGFWRGNLLSFNETRTTENNEGNASSPTVAAALFNANLRMIMLNGSGA
                     HTHVITNFILSNVSSNENGTTTYTGNSTISMPDAPIVDVPTTIKISGEVISIFPDPSK
                     VNEHFGNSPIYGAVPTGEEGIGRPPRGPGPFP"
     gene            complement(2737..3036)
                     /locus_tag="VE308_02375"
     CDS             complement(2737..3036)
                     /locus_tag="VE308_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZA99849.1"
                     /translation="MFCLGLSKVRFGIQAPHEQINPTDLLNDVIMMERYGIEKCWSSD
                     HYMPWWHTGATGGAAWPWLGAALAKTDRMIVGTGVTAPVLRYNQSGLFLTMYQST"
     gene            3120..3254
                     /locus_tag="VE308_02380"
     CDS             3120..3254
                     /locus_tag="VE308_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99850.1"
                     /translation="MSSSSAGNNELRCNECGITFTTVQDKEQHMKLEHKESKGPAGVK
                     "
BASE COUNT          962 a          712 c          703 g          922 t
ORIGIN      
        1 taacaaggtt aagccatgtt ttaataattt gttgatatca tcttgaacgc catccgcagc
       61 agcatatgga gaagcaggat ttggcttggc cctgtcctga ccctttaagc aaatctatcg
      121 ccaatctatc ctaaaatcct tccaagggca gggccattat caatagatca acactttttt
      181 gcttcttgct cgctagcctt ttataaactt cagcatgtcc ctaagacata gcctagagat
      241 atctttcggc taaatgagat tccatggaac tctgttgcga ctatctgaaa ttgaagggag
      301 gagcattata accgattgct gtcaaacgta tacgtggctg cgatggaagt ttcacaagca
      361 agtgttccaa tctctaacgt aatatctatc tacagttagc atagtgcagc tgcaatcttc
      421 ttaagagtac acaaatgttt gtcaaccact tttagaaata ttcacaagat ataacatgaa
      481 atggcaaagc ccgctatgac aaatgactta cggattttca gagtatttgt cattattcta
      541 tttgtaatta ttattatagt actatgtcgg atgtggcctg taataatgat aagctaggat
      601 atgttcagat atatgcatct catagagaaa ttatattgaa actagataca taagtcctct
      661 aataatctct taaatgcaaa catgccacta aattcgtaat gaaggttact ctttttcaag
      721 ttattcccat tgctgccttg gttttgtctg tgtatgcact gtcgacacca ccaccatcat
      781 cattatttgc acagcagcag cagcagcagc agactactag caattactca tttgtcacag
      841 cgtggggtag ctctggaagg gcatttggtc aattcagcca accttttgat gttgcaacag
      901 attcagcagg taatgtctat gtgactgatt caactgcttt gtcaaatcaa gtgcagaaat
      961 tcacttcaga tggaacattc atcctttcat ggggctccct tggaactgga gcaggaggtt
     1021 ttgcaaatcc agctgccata acgatagatt ctaacggtat tgcatatgta acagattggg
     1081 gtaacgcaga gcaagcagtg cagaaattca catcagatgg tagttttcta agcatgtggg
     1141 gttctactgg attaggtgag ggaaggttca taacccctgg cggcgttgca gtagactctc
     1201 aaggctttgt gtatgtaggt gactttgggg agaacaacaa catacaaaag tttgatagcg
     1261 atggtaattt catcttaagc tggggaagcc cgggttcaaa tgatggtcaa ttcacgaatc
     1321 ctgcagctat agccatagac tctcaaggcg ttatttatgt cgtcgacaac ggaaatgatc
     1381 gcgttcaaaa gtttgacacc aatggaaact ttctaggaaa gtggggaaca tcaggttctg
     1441 gagatggaca atttaactct ccggcaggaa tagcgataga ctcgtctgat aatattttca
     1501 tagtagacag agccaatgat cgcgttcaag tttttgaccc caatggtaat ttcataacta
     1561 aatggggctc acgaggctca gctgatggcc aatttaataa tcctatagga gttgccatag
     1621 gcacagacgg aagggtctat gtgactgatg aagggaatgc cagaatccag gtgtttagta
     1681 gaggaggagg cggctaaaat tagttcattc gacatgatac aacgaacctt tctgcttttt
     1741 atttcaaaca ttcatataca atttggcact gttccgattg ggatgagaaa gagcttctta
     1801 cgttctgttt tcattgctat cctgttttca gctttagtta tagtacctat ttccctgggt
     1861 gtaactcctg tttatgcaca gggacctccc attgcacctg ataataatca aggactacct
     1921 ccaggacccc caccaaggtt tcctcttcca tttgatcaac aatcaccatt tgatcaagca
     1981 agcaattttt cctttggacc aattgctagt attcagaata acgagtccgg ccaacctgcc
     2041 tgggttgttc taggattctg gagaggcaac ttgctttcat tcaatgagac aagaactact
     2101 gaaaataacg agggtaatgc ctcaagcccg acagttgcag cggcgctatt caatgcaaat
     2161 ctcaggatga taatgctcaa tggatcagga gcgcacaccc atgtgatcac gaattttata
     2221 ctgtcaaatg tttcatctaa tgaaaacgga acaacgacgt atactggcaa ttctacgatc
     2281 agcatgcccg atgctccaat tgtagatgta cctaccacca ttaagatatc aggtgaagtc
     2341 ataagcatct tcccagaccc ttcaaaagta aatgagcact tcggtaacag ccctatctat
     2401 ggggccgtcc ccacagggga agaaggtata ggacggccgc ctcggggtcc tggaccattc
     2461 ccttaatttt ttgaagttca agtggtcaga gaagattttg ttgtcgtgga gaggttgctg
     2521 ttaatttctt gagatgacga tgcatgtaag tatctcgaga gatagaccaa gtttgagatg
     2581 tacatacatt ttctctttac cacaatattc ttctcgtgat aggatcaatg ataaggtcag
     2641 caaccagcag catatcttcc tcccttggca gctctaaaac aaacgctgaa ggcttgctgg
     2701 cacctggccg ccaatgctgc aggcccgctt aatcttttat gtcgactgat acattgtcag
     2761 aaaaagaccc gactggttgt aacgtaaaac tggagcagta acccccgtac ccactatcat
     2821 tctatctgtt ttggcaagcg ctgccccaag ccaaggccaa gctgcgccac ctgtcgcccc
     2881 agtgtgccac caaggcatat aatgatcgct tgaccagcac ttctcgatac cgtatcgctc
     2941 catcatgata acatcgttca aaaggtcagt agggttgatc tgctcatgtg gcgcttgaat
     3001 gccaaatctt accttggaca gtcccaggca aaatatctat tataaattgt accatgccag
     3061 gaggtaaggg aaatttataa aaacacactt attctataaa agctacctga taatgtttca
     3121 tgtcatcttc atcagcaggc aacaatgagt tgagatgcaa cgagtgtggc atcacattta
     3181 caacagttca agacaaggag caacacatga agttagaaca caaagaaagc aagggtcctg
     3241 caggggtcaa gtaagcagat aacgctcaaa gtaatcagtg gcaggcgccc agcctcttc
//
LOCUS       DASQTF010000099         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1041416,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000099 DASQTF010000000
VERSION     DASQTF010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041416"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1184)
                     /locus_tag="VE308_02385"
     CDS             complement(165..1184)
                     /locus_tag="VE308_02385"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HZA99851.1"
                     /translation="MNNQEILFSDSILTLGGLDIGESVSKCLAPKLDVLKGKKIVFCY
                     DEKKMMPVDGWGMRSDPYNTTAVTTIRPIKENVLVSAIDSSSVKLAETEDGSLYAIKC
                     GVATAYASHTLMHFKIGPVLFYLSEKTVHESELEERLSRLVLLDDDLAKRLIRVRVER
                     AVQKEIASHFTNSIILIDGSLKASLFEERERSISKISESSVLRKNMLVGISKSTKLKA
                     LDRAAAPLTKVPGPAYIEVDDIIKSLIRNTIGSNLMIKLEKGSSPILRADIVGDVSKS
                     LGMLLGNDMVAGGYPETLRLAHHISTFTSTEVTCLRSHILNNYDVTELAAEDIRRTLL
                     GSIPV"
     gene            1294..1791
                     /locus_tag="VE308_02390"
     CDS             1294..1791
                     /locus_tag="VE308_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99852.1"
                     /translation="MSGQAAEEVKIRLKHGEWEVEVVCIESRVKQVVESVLSSLDATK
                     MIGPEVQSQLDALRREIDSLKTRPITELVPEGQQQQKVTTKSGMTCRGLLENLWLEGH
                     FVNERPLGEVHEELSRRGYNYDRTAVSHSLTDMVRENILTRIGTMRNYRYIQKRPPNN
                     ITAQA"
     gene            complement(1775..2290)
                     /locus_tag="VE308_02395"
     CDS             complement(1775..2290)
                     /locus_tag="VE308_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99853.1"
                     /translation="MSQAENDVNVIVTIGDVRVEFSGSAETVTASVMNFLHKQVPSMD
                     LARKILLNYAAQELIETYSHVIKITPEGPRVILDADVKLSDKDIVALQLVAAKIAKDL
                     GKIQDNSMQVSDIHAATALNPKSISSRISELVKAGHVARDEKESSKYRITTAGIHWLN
                     STIAKKIRPAP"
BASE COUNT          611 a          539 c          521 g          672 t
ORIGIN      
        1 tacaatgtct tcaatcaaag cctgggatga aaggtactct tcatcataga cctgtacaac
       61 catgctcctg ctcctgtcct ctatgagtaa gtagtctccc ttggcagctg aatcttcctt
      121 catggcaagt aagagcagtt cattgccatt ctttgatagt atcttcatac tggtatcgac
      181 cccaaaagag ttctgcgtat atcctccgca gctagctccg tcacgtcata gttgttcagt
      241 atgtggcttc ggaggcatgt aacctctgta cttgtgaacg tggaaatgtg gtgtgcgagc
      301 ctgagggttt caggatagcc acctgctacc atatcattcc caagcagcat gccaagtgac
      361 ttgctaacat cacctacaat gtctgctcgc aaaataggac tgctgccctt ttccaacttg
      421 atcatgaggt tgctgccaat agtgttcctg atcaagctct tgatgatatc atcaacttcg
      481 atgtaagccg gccctggcac ctttgttaat ggtgcggccg ccctgtctag cgccttgagc
      541 ttggtgctct tgctaatccc tacaagcatg ttcttgcgaa gtacactgct ctctgaaatc
      601 ttgcttatgc tgcgctccct ctcttcaaag agagatgctt tgagcgagcc atcaattaga
      661 attattgaat ttgtaaagtg gcttgcaatc tctttctgaa cggctctctc tacgcgtact
      721 cttatcagac gcttcgccaa gtcatcatct aatagaacaa gccttgagag ccgctcttcc
      781 agctctgatt catgaactgt cttttcgctt aagtagaaca acaccgggcc tatcttgaaa
      841 tgcatgagag tgtggctagc ataggccgta gcgacgccac acttaatagc gtaaaggctc
      901 ccatcttctg tctcggctag ctttacactg ctagaatcta tagccgacac aagcacattc
      961 tctttgatcg ggcgaatggt agttactgct gttgtattgt aggggtcaga tctcatgccc
     1021 catccatcaa ctggcatcat tttcttctca tcatagcaaa acacaatttt cttccctttc
     1081 agtacgtcaa gctttggagc aagacatttg ctcaccgatt cccctatgtc gagaccccct
     1141 agagtcaaga tgctgtctga aaaaaggatt tcttggttgt tcatgttgat caacaatatc
     1201 tgcgggcaca agtatattaa tattgctcaa catgatcaat atggccagca acagatttat
     1261 caatagcagc ctgaaaggta tatttaagca aatatgagcg gccaagctgc tgaggaagtc
     1321 aaaataaggc ttaagcacgg tgaatgggaa gtagaggtag tctgcattga gagcagagtc
     1381 aagcaggtag ttgaaagtgt gctctcaagc cttgacgcta ccaagatgat agggccagaa
     1441 gttcagagcc agctcgatgc attgaggcga gaaatagact ccttaaagac taggcctatt
     1501 actgagctag taccggaggg ccagcaacaa cagaaggtga caaccaagag tggtatgacc
     1561 tgtaggggtc ttttggaaaa tctctggctt gagggacatt ttgttaatga aaggcctctt
     1621 ggtgaagtac atgaagagct atcgaggaga ggctacaatt acgataggac tgcagtttcg
     1681 cattcgctta cagatatggt tcgagaaaac atattgacaa gaattggaac aatgaggaac
     1741 taccgctata ttcagaaaag gccacccaac aacattacgg cgcaggcctg atcttcttgg
     1801 ctatcgtaga attgagccag tgaatgcctg ctgtagttat cctgtatttg cttgattctt
     1861 tttcatcacg cgcaacatgg ccggccttga ccaattccga tattctggag cttatggact
     1921 ttggattgag tgccgtagcc gcatgaatat ctgacacctg catagagttg tcttgtatct
     1981 tgccaagatc ttttgcaatt ttagcagcta caagctgcag agctactatg tctttatcag
     2041 acagctttac atctgcgtct aaaattacac gaggaccctc tggtgtaatc ttgatgacat
     2101 gggaatatgt ttcgatgagc tcctgcgctg cgtagtttag taggatcttt cttgcaagat
     2161 ccattgaagg tacttgctta tgcagaaagt tcattacaga agccgtgacg gtctctgcag
     2221 agccgctgaa ctcaactctc acatcgccta tagttacgat gacattaacg tcattttcag
     2281 cttggctcat tatcttcttt tcgcttgtaa cacaaatggc gggctatata ttgaattcgt
     2341 tag
//
LOCUS       DASQTF010000100         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_582705,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000100 DASQTF010000000
VERSION     DASQTF010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582705"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..733)
                     /locus_tag="VE308_02400"
     CDS             complement(137..733)
                     /locus_tag="VE308_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HZA99854.1"
                     /translation="MISVIEGAELVRLARSAVEKYLQESVIIKSEKDMLQKAGIFVTL
                     NYITRSKKEYLRGCIGFPLPEKPLYQGVIEAAIAAATEDPRFPPLDRQELDNILFELS
                     VLTPPQEIRISPTDYGKEIEIGRDGLILQWRFGSGLLLPQVPVELKWNVEEYLANICY
                     KAGAPPDVWLDPTSKLFRFQAAVFKEVEPRGKVIRVEI"
     gene            1512..1655
                     /locus_tag="VE308_02405"
     CDS             1512..1655
                     /locus_tag="VE308_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99855.1"
                     /translation="MVWLNGSVLHDPPVKQYECRGCQLFVVKYQDGNYEIVPIDPEMQ
                     QQQ"
     gene            1658..2068
                     /locus_tag="VE308_02410"
     CDS             1658..2068
                     /locus_tag="VE308_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZA99856.1"
                     /translation="MKINKVVETCIYSSDLESMKKFYVGIMGLSVIQEEENKLIFLKA
                     GKNMLLIFDPLRTSISNDKLPTHGAMTPPSSIHFAMEIEEQDYQPWKDLLTRNGIAIE
                     KEVNWNSKTKSLYCRDPSGNLVELITPGGWPVES"
     gene            2123..2968
                     /locus_tag="VE308_02415"
     CDS             2123..2968
                     /locus_tag="VE308_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HZA99857.1"
                     /translation="MREQLEDRNKIIVLPGVFDALSAKIAEHVGFEAMFQTGYGSSAA
                     LLGMPDFGLLNSGETVDNALRIIRAVRAPVLVDADTGYGNPLNVWRLVRDLESLGAAG
                     IFLEDQVWPKRCGHMVGKDVIPKVEYIPKLKAALEARQSKDFIIVARTDARAPMGLDE
                     AIERGKAYRKAGADVIFVEAPRSIEELKKVANEIDAPLVANMIEDGVTPNLPASELLK
                     LGYQIAVFPLSAIYSATFAMRQVLTELKNTGTTNDARKIMVTFKDFNQFMDLEHFTDL
                     ERRYA"
     gene            complement(2990..>3503)
                     /locus_tag="VE308_02420"
     CDS             complement(2990..>3503)
                     /locus_tag="VE308_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99858.1"
                     /translation="ADRWIILTGIFLSIFAGYGILHLINKLKSNLSAIVACSIIAAFA
                     GIGLAYALMPYDSPFILYGGVQNYIARFAPATMQFNSLDIQDNDNLLSAIAQINENTE
                     HNAIIVGEPHWRGFMELYLKDDRIYHFSNDPLTFAKSLEQQGHEVYLIKIEGNLQTSF
                     RIENIQKDEV"
BASE COUNT         1019 a          748 c          775 g          961 t
ORIGIN      
        1 acatctactc cctcttgctt gaggcgatca gccaccataa gcaatcggct agcatgccca
       61 aggcctacac catatggaga taagtatgct actggtgaca aggggctcaa atatcactgc
      121 tgattatata ttattgtcat atctcaaccc ttatgacctt accccttggt tcgacttctt
      181 tgaacactgc tgcttgaaac ctgaaaagtt ttgaggtggg atcaagccat acatctgggg
      241 gggcgcctgc tttgtagcat atgtttgcaa ggtattcctc aacgttccac tttaattcga
      301 ctgggacttg cggcaggagt aaccctgagc caaaacgcca ttggagtatt aggccatctc
      361 ttcctatctc gatctctttt ccatagtcgg tagggctgat tctgatctct tggggaggag
      421 taagtacgct tagctcgaaa aggatattgt caagctcctg tctgtcgaga ggaggaaagc
      481 gagggtcttc tgttgctgct gcaattgctg cctctatcac cccctgatat agtggtttct
      541 ccggaagcgg aaagccaatg cacccgcgca aatattcctt tttgcttctg gttatatagt
      601 tgagcgttac aaaaatcccc gccttttgta gcatatcctt ttctgatttg attatgactg
      661 attcttgcaa gtatttttcg accgcgcttc tggcaaggcg gacaagctca gcaccctcga
      721 tgactgatat caatcctgct ttattcactc tagacggact aaaaaatcca cagcttccat
      781 agcatatttt cttccatctt ttcgaatgtg aaagaccacg ctctgaagat acagatctgt
      841 ctcaattaca ttacgaaatt cttccacgcc tcttggcatc gctggataac aagataaggc
      901 gacatttagc ataaacacaa acaaactact gtactgctgt gaaatgaagg agagttctca
      961 cataagcgac aagtgtataa tgataggcat gccctcaccg ttgagggcaa agaattgttt
     1021 ttatgtcgag tacatatata gcgagattat aacatatact gctcagcaac gatgtgcatg
     1081 cttttggcta tcattcctgc tatgatatat agaatttcat aagggcacag tttttaaaga
     1141 aatgctctgg gtcaggagac tttccgcatg ttaatgcata gaatccaatt aaaccgtact
     1201 atcgaggttc aatatacata tcttaaacaa gagagaaaaa aatcgcccgc tgtacataga
     1261 acatgcatat tgtttatggc gtgctgtctc ttaattttac tccggaacta gccgcttttc
     1321 gaggattgga gggcatgagg atgtaaaaca gcaacaaatg ccgcttaccg ttgtattttc
     1381 tagatacaac tagatacaca tgaaatagct tttccaggcg ccaatattac accagcaata
     1441 agacatatta acactaaacc ctcaatctaa tccacatttc tatgatgaat tgtcctaact
     1501 gcggtgcaat catggtatgg cttaatggtt ctgtactaca tgacccacct gtgaagcaat
     1561 acgaatgcag aggatgccag ttattcgttg taaagtatca agacggcaat tatgagatag
     1621 tgcccattga cccagagatg cagcagcaac agtgataatg aaaataaaca aggtcgttga
     1681 gacatgcatc tattcttcgg atcttgaaag catgaaaaag ttttacgtag gaattatggg
     1741 tttgtctgtt attcaagaag aagaaaataa gctgatcttc ttaaaggccg gtaagaacat
     1801 gcttttgata tttgatcctt tacggactag tatcagtaat gacaagctac ctacgcatgg
     1861 cgcaatgacc ccgccctcga gtatccactt tgctatggag attgaagagc aagattatca
     1921 gccatggaag gatctcttga caaggaatgg tattgcaata gaaaaagaag tcaactggaa
     1981 tagcaagact aagtcgcttt actgtcggga tccgtcaggg aatcttgtgg aattgataac
     2041 gccgggcggc tggccagtgg aaagctaata taatattctc ccccttcatt tctaactgat
     2101 gtataataaa acacgggcct ctttgagaga gcagcttgaa gacagaaata agataattgt
     2161 gctgcctggc gtttttgatg cacttagcgc caaaatagca gaacacgtgg gcttcgaggc
     2221 aatgtttcag acaggctatg gctcgtctgc agctcttctg ggaatgcctg attttggatt
     2281 gctaaactcc ggagaaacag ttgataacgc tttaaggata atccgagcgg taagggcccc
     2341 agttctggtt gatgctgata cggggtatgg aaatcctctc aacgtctggc ggcttgttag
     2401 agaccttgaa agcctaggtg cagcaggcat ctttcttgaa gatcaagtct ggccaaagag
     2461 atgtggccat atggtgggca aagatgtcat cccaaaagtt gaatacatac ctaagctcaa
     2521 agcggcgcta gaagcgagac agagtaagga ctttatcatc gttgcgagaa ctgatgcccg
     2581 tgcgccaatg gggcttgacg aagcaatcga gcgtggcaaa gcctacagaa aggctggcgc
     2641 cgatgtcata tttgtcgaag caccgaggtc gatcgaagag ttgaaaaagg tggccaatga
     2701 aatagacgcc ccgcttgttg caaatatgat agaggatgga gtgactccaa acctacccgc
     2761 aagcgagcta ctaaaacttg gctaccagat cgcagttttt cctctatcag caatctacag
     2821 tgccacattt gcgatgagac aagtgctcac ggagttaaaa aatactggca caactaacga
     2881 cgctcgaaag atcatggtta ccttcaagga ctttaaccag tttatggacc ttgaacactt
     2941 tacagacctc gaaagaaggt atgcttgata tatctaaatt aaaaagcggt tagacctcat
     3001 ctttttggat gttttcaatc ctaaaacttg tctgcaggtt tccttctatc ttgatcaaat
     3061 agacttcgtg cccttgttgc tccaaactct tagcaaaagt cagcgggtca tttgagaagt
     3121 gatatatcct gtcatctttt agatacaatt ccatgaaacc tcgccaatgc ggttcaccta
     3181 caataatggc gttatgctca gtattttcgt ttatctgtgc gattgccgat aatagattgt
     3241 cgttatcttg tatatcgagt gaattgaact gcatcgttgc aggcgcaaag cgtgcgatat
     3301 aattttgcac accaccatag aggataaacg gactgtcgta gggcatcaat gcataggcca
     3361 agcctatccc agcaaatgct gcaattatag agcaggcaac tatcgcagaa agattagact
     3421 tgagcttgtt gataagatgc aagatgccat agccggcaaa gatcgagagg aaaatgcctg
     3481 ttaagattat ccatctatct gca
//
LOCUS       DASQTF010000101         5556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1042156,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000101 DASQTF010000000
VERSION     DASQTF010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042156"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VE308_02425"
     CDS             complement(<1..617)
                     /locus_tag="VE308_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99859.1"
                     /translation="MRLVDTFKASLDPYLFRLYRRYLYSRYKATDELAQAEVVHEKAK
                     ESLLKMTDKFMQNRTYFDPGDQLVVEIENHRLKSPVGIAAGFDKNCDVLRPTSYVFGF
                     LNPGSVLKNPNTGNPQRPKSKGTVRIVVDDKRKAIINAQGYPHKGLDYAISNLRKFME
                     GSRGNAKILLNFSGITDIPTEDAVLDSCKEILVRSAPHVDFGFEE"
     gene            complement(692..865)
                     /locus_tag="VE308_02430"
     CDS             complement(692..865)
                     /locus_tag="VE308_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99860.1"
                     /translation="MKRKRTRAIRILLAKKSTMIIPKAGVLLMVKIREGYYRSRRYEI
                     SDRYYFDNGRADL"
     gene            complement(921..1292)
                     /locus_tag="VE308_02435"
     CDS             complement(921..1292)
                     /locus_tag="VE308_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HZA99861.1"
                     /translation="MKIYSESRPWGKFEKFHENKSCTVKLIYVNANSRLSLQYHKKRS
                     EFWKVIKGSAMVEIDEKTIILEEGETITIPRQARHRVLARESECIILEIAYGRFDEND
                     IVRIEDDYQRVTMPKTRVAAA"
     gene            1770..3776
                     /locus_tag="VE308_02440"
     CDS             1770..3776
                     /locus_tag="VE308_02440"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HZA99862.1"
                     /translation="MNQKATVEVAAAQSKRAVMKGKVRYDRKAESEFFVDNSALAGFT
                     TERILYMAVRELIENSLDSCETGHILPKISLSLKLLDASNDLWMITCQDNGIGIPSDK
                     VPIAVCSFLTSGKYVEKQQRGLFGVGLKMIAAFSTKDTIHPLKVWSKSIDEEGLEYHF
                     ALRTDISTNKPIVLAKKVIKGEESKIDGDSGFKVEAVLHAKLSPITRNNIKNKINEYI
                     SQTSIVNPYAIIEYETDEGKVKFERRTEVMPQPAKEVLPHPADMDLQTLKKAIMNFMN
                     QKTTLQGILSTSFQKMSSEKAKEIIAKAGVENKSADKYTENELIEIVKVCKYINFQQA
                     NTDHLSPIGEQILTTGMTSEYTIISTKETVSKAASIAEIQRPQITVKILKPALAAYAS
                     RTCVINNRPTIVECGIAYGGDIPSFKLYRFANKIPLLYDEGSDVAREVVSEVEINKMG
                     ITKKEAKAQFANGEIKSDRAVELLPIHTFFHICSTKIPYKTAGKESIASEGELKKHMK
                     YCLSELYRKVSAQIRKELRMKEAQGRLNLYKYYIPLVVSAISESIRLDAAKLEQAFTE
                     LAEKHVKGEITGGLVEKKEDRITGERLEEEAEEALEVNGEVVKPDKEQIAITTARRKG
                     KLTKPRINEEKIPETLRKDKKQDQAQMTLDKVAEDAKPKARKSK"
     gene            3777..4880
                     /locus_tag="VE308_02445"
     CDS             3777..4880
                     /locus_tag="VE308_02445"
                     /inference="COORDINATES: protein motif:HMM:NF044723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99863.1"
                     /translation="MPADKRHLEVIKKIRLIMKDVSTDLMAQKTMPVLNVPKVGYDNT
                     VWSGEERMLTIGDKTVSISPDSTKKIPTFAQYLVMANAVRKLLNEEMESTIRGMYYAT
                     LSTVGDEKNKQKFWNSQDDSDDAIKAIELLTGVPREEFSVTSKPKGMISGPITLRVGG
                     DIINCNLGSRATSQLIPTNIRDVEIVDVKADFVMVVEKDTVLNNIRKSGFIQKHNAIL
                     MTGSGEPDRATRMMVKALNDEWKKPVVVFADADPWGLGIALRYKIGSESLSYDSHRLV
                     TPDAKILGMLFSDIYEYNIPEVARLAASEEDINRANDMKKKPWFQDKKWQKELDIFLK
                     NKEKCELDAFFKHGFKYLSENYLPRKLREAGVI"
     gene            complement(5317..>5556)
                     /locus_tag="VE308_02450"
     CDS             complement(5317..>5556)
                     /locus_tag="VE308_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99864.1"
                     /translation="FNKGVRTQDEEHIGHVMTEAGDKIVVWGHYNWRFDVPKSKIIAV
                     GRNVILGMDYNDMFKYKVDRNAPLPTGEPVERLAE"
BASE COUNT         1698 a         1223 c         1224 g         1411 t
ORIGIN      
        1 ttttcttcaa acccaaagtc gacatggggt gcagatctaa ctagaatttc tttgcatgaa
       61 tcaagcaccg cgtcttcggt agggatgtca gtaatgccgg aaaagttgag caggatcttg
      121 gcattgcctc ttgaaccttc catgaatttt cttaaatttg aaattgcata atcgaggcct
      181 ttatgaggat acccttgtgc gttaattatt gccttccttt tgtcgtcaac tacaatcctg
      241 acagtgccct tgcttttggg ccgctggggg ttgccagtat taggattttt cagcacgctg
      301 ccagggttca gaaagccaaa aacatacgat gttggcctaa gcacgtcgca gttcttatca
      361 aacccagcgg caatcccaac tgggcttttc aatctgtgat tttcaatttc aactaccagt
      421 tggtcaccgg gatcaaaata tgtcctgttt tgcataaact tgtctgtcat cttgagcaga
      481 ctttcttttg ccttctcgtg cactacttct gcctgtgcaa gctcgtcggt tgctttgtat
      541 cttgaataaa ggtatctgcg gtaaagcctg aagagatatg gatctaaact tgccttgaag
      601 gtgtccacaa gcctcattta tggcctgcca gtcctttcaa cttcaacctt cctattcaca
      661 gccagtacta gctgttggtg atggctaact gtcaaaggtc tgcccgtcca ttatcaaagt
      721 aatatctgtc agagatttcg tatctccttg agcggtaata gccttcccta atcttgacca
      781 tcaacaacac accagccttt ggaattatca tcgtgctttt ttttgctaaa agtatacgga
      841 tagcccttgt tctctttcgc ttcattattt tgaactctac tatagccgaa ggttctgtca
      901 ctatttatgt gcatatccgc ttaggcagct gctactcggg tctttggcat ggtaaccctc
      961 tgataatcat cctctattct gacgatatcg ttttcgtcaa acctgccgta tgcaatttca
     1021 agtattatgc actcgctctc ccgcgcaagt actctatgtc tcgcttgcct tgggatcgtg
     1081 atcgtctcac cctcttcgag gataattgtc ttttcatcaa tctcgaccat agcagagccc
     1141 ttgatcacct tccagaattc agaccgcttc ttgtggtatt gcagactgag tcgagaattt
     1201 gcattgacat aaatcaattt gacggtgcag gacttgtttt cgtggaactt ctcaaatttg
     1261 ccccacggcc tgctttcaga gtaaatcttc atataagaaa aaggaagaaa attgatttaa
     1321 ctatttccat tcactataaa tactatatat tgagaaaaat tagctgtggc caagtcagac
     1381 gattgcgtca aaaatgagca tggattgtgc agtggtatca ttaaagaaat agtcaactca
     1441 ttcactatga ccaagatgtg tgcatgcccc tgccatgaca gtatgtacca tctcattaga
     1501 aatactatcg cagtcgtgaa ccagaaccag aggaacaatc cctaccagtt cacccatgat
     1561 aatgactaca cataggccac gaggttagct ttttctatac ttgaaattca agcagaaccg
     1621 ttctgatatt gtaagtattt tagatcaact atcaacaaga aagaaaattc tcaagggatc
     1681 ccaataaact agattaatct ttactatcta ttggctcaac aaatttttca taaaaagtta
     1741 aattaagtta gcgaaaagtc tgaatgactt tgaatcaaaa ggctactgta gaggttgcag
     1801 ctgcacagtc aaagcgggca gtcatgaaag gaaaggtaag gtatgacaga aaagccgagt
     1861 cggagttctt tgtcgacaat tcggctctcg caggctttac cacggagcgt attttataca
     1921 tggctgtaag ggaacttatt gaaaactcac ttgactcgtg cgagacaggc catatcctgc
     1981 ccaagatatc gctgtcgctc aagctacttg atgcatcaaa tgacctctgg atgattacat
     2041 gccaggacaa tggtatcggg ataccgtcgg acaaggtgcc cattgcagta tgctcgttcc
     2101 tgacatcggg caaatatgtt gaaaagcagc agcgcggcct gttcggtgtg ggcctcaaaa
     2161 tgatagctgc gttttctacc aaagatacta ttcacccact caaggtctgg tcaaaatcga
     2221 ttgatgagga aggattggaa tatcatttcg cgcttaggac agacatcagc acaaataagc
     2281 caatagtcct tgcaaaaaaa gtaatcaagg gcgaagaaag caagattgac ggagactctg
     2341 gctttaaggt tgaggcagta ctccatgcaa aactttctcc aattacaagg aataacataa
     2401 agaacaagat taacgaatac atcagtcaga caagcattgt caatccttac gctattattg
     2461 aatatgaaac tgatgagggc aaggtcaaat ttgaaagaag gacagaagtc atgccgcagc
     2521 ccgccaaaga ggttctaccc cacccagcag acatggatct acaaacactc aagaaggcga
     2581 taatgaactt tatgaatcaa aagaccacac ttcagggaat cttatcgact tcatttcaaa
     2641 agatgtcaag cgaaaaggca aaagagatta ttgctaaagc aggagtagag aacaagtcgg
     2701 ctgacaagta caccgaaaac gagctcatcg aaattgtcaa ggtgtgtaag tacatcaact
     2761 tccaacaggc aaacacggat cacctgagcc cgattggtga acagatcttg acgactggaa
     2821 tgacctctga atatacaatc atctccacca aggagactgt atccaaagca gcatctatcg
     2881 cagaaatcca acggcctcag atcacagtta agatactgaa gccagcacta gctgcctatg
     2941 catcccgcac atgtgtcatc aacaacaggc ccaccattgt cgagtgcgga atagcatatg
     3001 gaggtgacat tccctcgttc aaactctata gatttgcaaa caagattcct ctcctgtacg
     3061 atgaaggatc agacgtagca agggaagtag tttctgaagt tgagataaat aagatgggga
     3121 tcaccaagaa agaggcaaag gcgcaatttg caaatggtga gatcaaatcg gacagagccg
     3181 tagaactttt gccaatccat acctttttcc acatttgctc taccaagatc ccgtacaaga
     3241 ctgctggcaa ggaaagcata gcctcagaag gcgagctgaa aaaacacatg aagtattgcc
     3301 tgtctgagct ctatcgtaag gtgagcgcgc agataagaaa ggagctacgc atgaaagagg
     3361 ctcagggcag gcttaacctc tacaaatatt acattccttt ggttgtaagc gcgatttccg
     3421 agtcgatcag actcgacgcc gccaagctgg agcaggcgtt tacagagctt gcagaaaagc
     3481 acgtcaaggg cgagatcact ggcggactcg tggagaaaaa agaggatagg ataactggtg
     3541 agcgccttga agaagaagcc gaagaagcat tagaggtcaa cggcgaagta gtcaagccag
     3601 ataaggagca gatcgcaatc actactgcaa ggagaaaggg caagctgaca aagccgagga
     3661 tcaatgagga aaagatacct gaaactctgc ggaaagacaa gaagcaggat caagcgcaga
     3721 tgacacttga caaggtagct gaggatgcta agccaaaggc gaggaagagc aagtaaatgc
     3781 cagccgacaa gcgtcacttg gaagtcatca aaaagattag actgataatg aaggatgtca
     3841 gcactgacct tatggcacaa aagaccatgc ctgtgctaaa tgtccccaag gtaggctatg
     3901 acaacacagt ttggtctggc gaagaaagga tgttaacgat tggagacaag actgtcagca
     3961 taagccccga cagcactaag aaaataccca cgtttgccca atatctggtg atggctaatg
     4021 ctgtgcgcaa gctgctgaat gaggaaatgg aaagtacaat acgtggaatg tactatgcca
     4081 cattgagtac tgtaggcgac gagaagaaca agcaaaagtt ctggaacagc caagacgact
     4141 cagatgatgc aatcaaggca atcgagctat tgacaggtgt tccaagagaa gagttctctg
     4201 taacctcaaa gccaaagggc atgatatcgg gcccgatcac gctcagagtt ggaggcgata
     4261 taattaattg taaccttggc agcagggcta catcgcagct gataccgacc aacatacgcg
     4321 acgttgaaat agtagacgtc aaggccgact ttgtaatggt agtggaaaaa gacactgtgc
     4381 taaacaatat acgcaagtct ggctttatac agaagcacaa tgcaatcctg atgactggct
     4441 caggcgaacc agatagggca accagaatga tggtaaaggc gcttaacgat gagtggaaaa
     4501 agccggtggt ggtttttgca gatgccgacc cttgggggct tggcatcgcg ctccgataca
     4561 agatcggcag cgaatcgctc agctacgata gtcacaggct cgtcacgccc gacgccaaga
     4621 tacttggcat gctgttctcc gacatttacg agtataacat acccgaggtt gcaaggcttg
     4681 ccgcgtcaga agaagatatc aatagggcaa atgatatgaa gaaaaaacct tggttccaag
     4741 acaaaaagtg gcagaaagag cttgacatat tcctgaagaa caaagaaaag tgcgagcttg
     4801 atgcattctt caaacacggc ttcaagtacc tctcagagaa ttatctgccg cgaaagctgc
     4861 gcgaagcagg cgtaatttag tattattaaa tatcatgaag tttgccatat atgctatcac
     4921 aagtattaaa tgtaataatt tatgaaatgc ttgctgaagc cttctttctt tgtgcctcct
     4981 cctactgctg gtacaataaa atgcgtgcat gaggcatgcg gtgcagtcgc taggcgagat
     5041 aatgttaaga ttcttaacat taccctatac atcaactatg taagatgact actgctacag
     5101 tactggatcg atacaggact atcatgtctt atccttcaaa agctccttga agtttcgcta
     5161 caagaatagg aaagaacaac aacatcgtca cacatctatt tcatcctgca attctgaaag
     5221 aatgctgaaa agatttattc ttatattatt acatggaaaa gtgcagcagg cattacttca
     5281 cgatacatta agagccagaa gcttactgca ttttgttcac tcagctagac gttctactgg
     5341 ctctccagtg ggcagcggag cattcctgtc tactttgtac ttgaacatgt cgttgtagtc
     5401 cattccaaga ataacatttc tgccaacggc aataatcttt gactttggca cgtcaaaccg
     5461 ccagttgtag tgtccccata ccactatctt gtctccagct tcagtcatta catgccctat
     5521 atgctcttcg tcttgtgttc tgacaccttt gttaaa
//
LOCUS       DASQTF010000102         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1042207,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000102 DASQTF010000000
VERSION     DASQTF010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042207"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="VE308_02455"
     CDS             complement(<1..1573)
                     /locus_tag="VE308_02455"
                     /inference="COORDINATES: protein motif:HMM:NF018444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HZA99865.1"
                     /translation="MTSTILAYFPFHTVREKQKKVLDMIDSAIKSGYSQIFLEAPTGF
                     GKTPVAITLARYLGSSHICTATKDLQTQYRRDFPFVVDVKGRSNFPCIVKEDMGLDES
                     CNYGPCIKDDSYDCSYKTRLIDYKVEAQGTLHELVKLDPFAERKYNEKMRSKPKLIDL
                     DWRPCHYYHQKWIGGHATHTVYNYKYFLSDIFYAGATQKRKLLVLDEAHQLENEVGDF
                     RSFTVRKNMLPFLKMHMPDSKVDDIETWLDLCLKLKERLSEFTEKAQGIIERSNQKVT
                     LEPYTEKNLIDALELDKNLEVLTDDMKANKDNWIVSGVQRDSANQLVKVTLTPLDVSS
                     YFDTILDKGSVSLFMSATILSKDHLCKTTGLDHEKVKFIRIEDSDFPVKNRPIHMMNV
                     AWLSAKTMNASMPKIAQAVDNIMSIHRNEKGIIHTTSYSQLQFIRDKVSMENSSRLIE
                     TGSSLDRSEVLKKHYESKKQTVLISPSLHLGLDLKDDLSRFQIIVKIPYPDLTDKKIA
                     AKKDRDPKWYTWNTVL"
     gene            complement(1624..>2221)
                     /locus_tag="VE308_02460"
     CDS             complement(1624..>2221)
                     /locus_tag="VE308_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA99866.1"
                     /translation="KIDDKIAESLQQVGLDMSIAKRYPHELSGGMKQRVVIAMALLLN
                     PDILIADEPTTALDMLVQSQIINLLKKLQKEKGITIILITHDLALVSQITDRIGIMYA
                     GQLVEFSTTKDIYKNPRHPYTQALIEAIPRLRTEDKKIHFVKGSPPSLLSPPMGCRFQ
                     DRCVHAMDVCKRDPPEFETETGYVRCWLYEESEKNPQP"
BASE COUNT          532 a          479 c          487 g          723 t
ORIGIN      
        1 gtagcaccgt gttccaagta taccactttg ggtcacgatc ctttttcgca gcaattttct
       61 tatcagtaag gtctgggtat ggtatcttga caatgatctg aaatcttgat aagtcatcct
      121 ttaagtctag tccgaggtgt aaagatggag aaatcaggac tgtctgcttc ttgctctcat
      181 aatgcttttt cagtacttcg cttctatcaa ggcttgatcc tgtctctatg agtctgcttg
      241 aattttccat gctcacctta tctctaataa attgcagctg tgaataggac gtagtatgga
      301 ttatgccctt ttcgttcctg tgtattgaca ttatattgtc tactgcttgg gcaattttag
      361 gcatgctcgc attcatcgtt ttggcactga gccatgctac attcatcatg tgaataggcc
      421 tatttttgac tggaaagtct gagtcttcaa ttcggatgaa tttgactttt tcatggtcaa
      481 ggcctgtagt cttgcacaag tgatctttgc taaggattgt agcgctcatg aaaagtgaga
      541 cggaaccctt gtcgaggatt gtatcgaaat agctagagac atcaagcggt gtcagagtta
      601 ccttcaccag ttggttagca gaatcgcgct ggacgcccga cactatccaa ttatccttgt
      661 ttgctttcat gtcgtcagtg agtacttcaa ggttcttgtc gagttctagc gcatcaatca
      721 gattcttttc agtgtatggc tctagagtta ccttctggtt gctacgctct atgatgcctt
      781 gagccttttc tgtaaactct gatagccgtt cctttagctt caagcagagg tcaagccatg
      841 tttcaatgtc atccacctta ctatctggca tatgcatctt caagaatgga agcatgtttt
      901 tacggaccgt aaaactccta aagtcgccca cttcgttttc gagctggtgt gcttcgtcta
      961 gaaccaggag cttacgtttt tgtgttgcgc cagcatagaa gatgtctgat agaaaatatt
     1021 tataattgta aacggtatgg gttgcatgac cacctatcca tttttggtgg taataatggc
     1081 atggccgcca atctaggtca attaactttg gttttgatct catcttctca ttatatttgc
     1141 gctccgcaaa agggtcaagc ttaaccagct catggagagt tccttgagct tcgaccttgt
     1201 agtcaataag ccgagtcttg tatgagcagt cataagaatc gtccttaatg catggtccat
     1261 aattgcagct ctcatcaagt cccatatcct ccttgactat acagggaaag ttgcttcttc
     1321 cctttacatc gactacaaag ggaaagtcac gccggtactg ggtctgaaga tcttttgttg
     1381 ctgtacagat atgggagctc ccaaggtagc gggcaagcgt tattgcaacg ggtgtcttgc
     1441 caaagcccgt aggggcttca agaaaaattt ggctatatcc tgatttgatc gcggaatcga
     1501 tcatatcaag gaccttcttt tgcttttccc tgactgtgtg aaatggaaaa tatgctaata
     1561 tcgtgcttgt cattttttga aattacctac aggcagtggt gtctctttga cataccaggc
     1621 tttttaaggt tgggggttct tttcagactc ttcgtatagc caacatcgaa cgtaccctgt
     1681 ttctgtctca aactctggag ggtcgcgctt gcacacatcc atcgcatgga cgcaccggtc
     1741 ttggaatctg caacccattg gaggactcag caatgacgga gggctcccct tgacaaagtg
     1801 tattttctta tcctctgtcc tcaatctggg gattgcttca attaaagcct gagtatatgg
     1861 gtggcggggg ttcttgtaaa tatcctttgt agtggagaat tcgaccagtt gtccggcata
     1921 catgatacct atcctatctg ttatctgtga gacaagtgcc agatcatgag ttattagtat
     1981 tatcgttatt cctttttcct tctgcaattt tttaagcaag ttgattattt gcgactgtac
     2041 caacatatcg agcgcggtcg tcggttcgtc ggcaatcaag atatcaggat tcaacaaaag
     2101 ggccatcgca attacaacac gctgtttcat gccaccggag agctcgtgag gataacgttt
     2161 tgcaatcgac atatcaaggc caacttgctg gagggattct gctattttat catcaatttt
     2221 t
//
LOCUS       DASQTF010000103         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1223832,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000103 DASQTF010000000
VERSION     DASQTF010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223832"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..132
                     /locus_tag="VE308_02465"
     tRNA            48..132
                     /locus_tag="VE308_02465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84..86,aa:Leu,seq:gag)
     gene            complement(236..1129)
                     /locus_tag="VE308_02470"
     CDS             complement(236..1129)
                     /locus_tag="VE308_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99867.1"
                     /translation="MNPEMNNNDRKNHSKTLAIAGMGFLLIVAAFTFSFANSGQQLVN
                     AQQNQTQGGNATQTIVAVPFDTFTGHGVITASIGQGGQQQGGGNQTAGGGGGGNQTAG
                     GGGGGGGQEPQYILGGNWNLMAESGNVTDFTANFMMVHPDGTEYHTHNITNFVIGGSN
                     TVQLVQGQEATIAGVADIYTNGTNMWPGANTTLTLTQNGAVMTIMPAPDQTDNHFQGQ
                     PIYGIVSGMTGENGTMIVQTTPPGQQQEQQQQEEEGGLGGLLGGGEEGGQQQEGGNQT
                     GDGGPLSGITDPLQDLFGGGQ"
     gene            complement(1619..2158)
                     /locus_tag="VE308_02475"
     CDS             complement(1619..2158)
                     /locus_tag="VE308_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99868.1"
                     /translation="MSEGTPESSTETDEESLSRKARETGLTLKELANSIGKKGKAVAQ
                     QKSTQLKEAAKDDEINAKDAADIQMLGRHIESIITIFEDTMDKIMTHPSYDEKHGMLV
                     GFKKILEEEVNLIDARLSMAKRLKGLDPSKDSETLDERIEERKQTNAIDAELVNEPLP
                     NKDEARTMAEMVEDDRDKI"
     gene            complement(2216..>2441)
                     /locus_tag="VE308_02480"
     CDS             complement(2216..>2441)
                     /locus_tag="VE308_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99869.1"
                     /translation="QFSYVAAQVSVSGNEEILLLEMTGNNSTRVLNLSNNPKISECPS
                     IAIAGDNIYVVWEDMTPGNHEILYAKGIRA"
BASE COUNT          578 a          557 c          494 g          812 t
ORIGIN      
        1 gaccggaaat attcaaatta gcgcaaggca accgtgcttt catacgagcg ggagtcgccc
       61 agcatggcca aaggcgtaag actgaggctc ttatccctta ggggttcgtg ggttcaaatc
      121 ccacctcccg caccttttgc acttcaactt aaaactttat tgttcttttt gctgcctaca
      181 tttacagata gaaaatccta tagaaaaaat aaaaaagaaa aatacgcttt ggcggttact
      241 gtccgccgcc aaataggtct tggagtggat cggtgatacc actaagtggg ccgccgtcgc
      301 ctgtttggtt gccgccttct tgttgttgac cgccttcttc accgccgcct aggagaccac
      361 caagtccgcc ttcttcttct tgttgttgtt gttcctgctg ttgtcctggt ggcgttgttt
      421 gtacaatcat ggtgccattt tctcctgtca tccccgatac gatgccgtat atcggctgtc
      481 cctggaagtg gttgtctgtc tggtcaggtg ctggcattat ggtcatgact gccccgtttt
      541 gagtcaaagt cagtgttgta ttagcgcctg gccacatatt ggtgccattt gtataaatat
      601 ctgcaactcc agctatcgta gcctcttggc cttggactag ctgtacagtg ttactgccgc
      661 ctatgacaaa attggtgatg ttgtgcgtgt ggtattcagt accatcagga tgcaccatca
      721 taaagttggc tgtaaagtca gtcacatttc cgctttcggc cataaggttc cagttacctc
      781 ctaggatata ttgtggctct tggcctcctc ctccgccgcc gccacctgct gtttggttgc
      841 cgccgccgcc gccacctgct gtttggttgc cgccaccttg ttgttgaccg ccttgtccta
      901 tacttgctgt aattacgccg tggcctgtaa acgtgtcaaa tggcacggct acaattgtct
      961 gagtagcatt gcccccttgt gtttggtttt gctgtgcgtt tactaattgc tggccagaat
     1021 tggcaaatga gaatgtaaat gccgccacaa ttaggagaaa tcccatacca gcgattgcaa
     1081 gagttttaga atggttcttt ctgtcgttat tattcatttc cggattcatt attaaatctt
     1141 gacatggtat tgtacttatt aaccgttgta acaattcaaa atagaacaac attatgcaat
     1201 ttttatacat attcaaaatt atttccaaga gccgtctcat ttctaccgac atataagaga
     1261 aacaagagag ggatcattac tatactatat gtcctatatt caccgataaa tttcatgatc
     1321 atatgtgggt gcgtcctgcc cgatctcgaa agaagacccg cttatgttga tatgacatcg
     1381 ttcaatacct ctgtaaatgt ttcctatagc gggagtcaat tgtcctcttg ctgttgcatc
     1441 taggaatttt cttcactctt gctaaatact attcaaaaag tttactctct caaaaagaaa
     1501 aataaaagtt attagtgatt tatattggag agttaacgct gagcatacat catatccaag
     1561 taaaaagagg ttttactgtt gttggatatc gtccaaaaaa ggcaaaaaag caatcgacct
     1621 aaattttatc cctatcgtct tccaccattt cagccattgt cctggcttcg tctttgttgg
     1681 gtaacggctc gttcaccagt tctgcatcaa tagcatttgt ttgttttctt tcctcgatcc
     1741 tctcatctag agtttcagaa tctttcgaag ggtccagacc tttgagacgc ttggccatac
     1801 tcaaccttgc atcaatgaga ttgacttctt cttctaaaat tttcttgaat ccaacaagca
     1861 tgccatgctt ctcgtcatat gaaggatgtg tcattatctt atccattgta tcttcaaaga
     1921 tcgtgattat gctttcgata tgtcttccaa gcatctgaat gtctgctgcg tcttttgcat
     1981 tgatttcatc atctttggct gcctctttta gctgagtcga tttttgttga gcgacggctt
     2041 tgcctttctt tccaattgaa ttagctaatt ccttcaaggt cagtccagtt tctctggcct
     2101 ttctagacaa cgattcttca tcagtttctg ttgaggattc cggggttcct tcagacattg
     2161 tttgttcaca atcgttctat ccattatgat taaaagcagt ttggtatgat gattgctagg
     2221 ctctaatgcc ttttgcatac agtatctcat ggtttccggg agtcatatct tcccacacga
     2281 catagatatt atctcctgct atagctatag agggacactc agagatcttg ggattattgc
     2341 tcagattgag tactcttgtt gaattattac cggtcatttc tagtagaagg atctcttcat
     2401 tgcctgaaac agagacctgc gctgctacat aggaaaactg t
//
LOCUS       DASQTF010000104         5543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_944824,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000104 DASQTF010000000
VERSION     DASQTF010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944824"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VE308_02485"
     CDS             complement(<1..571)
                     /locus_tag="VE308_02485"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019816.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZA99870.1"
                     /translation="MNLADNYDQNDSKSSSTDFKVTPWEVEGEVNYGKLIVKFGTQPI
                     TPDLLQKIKSMTGEIHPLLKSGYFFSHRDFDWLLDKKRRGESFYLYTGRGPSGMVHMG
                     HLMPWIFTKYLQDKFDSKLLFQLTDDEKFLYGQERTMKELESYTYENILDIIAIGFNP
                     KRTKIIVNTKHIQHLYPIAIEVAKRITFST"
     gene            634..2115
                     /locus_tag="VE308_02490"
     CDS             634..2115
                     /locus_tag="VE308_02490"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZA99871.1"
                     /translation="MSSAPTFHPIERSILNSLSNNDHVRAESLVASTGLSIDQIRRGI
                     EWLKFKNLISIKESSTQRVFVASEGMAAAKNGLPERRLVNSLEEGKHTIPEVLASGAI
                     KNEEINAAIAGARRNHWIQLVQGRMIATPDTTRPSPEERFIEKLKGGIEISKLTEEEK
                     HVLEQLKKRPNYIEIKEKKELRISLTDGARRIIPTVNQEMLERRLTTELITSGKWKET
                     NFSSLDVEAPSPTIYPGRRHPLVDIIEEIKEIFTGLGFSEIEGSIIQSGFWNFDALFT
                     PQDHPAREMQDTFYISAQMQPISASKDQISKVSRVHNERWNRQWDIQEAQRLVLRTHT
                     TPVTIQHLAKIKSDVGRFFSVGRVFRNEKVSYKHLVEFHQVEGVATSEEASLRDLMGL
                     QKEFYAKMGIKRIKFWPTFFPYTEPSLQSMVYNEKLEKWVELFGMGIFRPEVTKPLGI
                     KNPVLAWGGGLERIAMLRFGLDDVRELYGNKLGWLRSVPKCQL"
     gene            2082..3752
                     /gene="pheT"
                     /locus_tag="VE308_02495"
     CDS             2082..3752
                     /gene="pheT"
                     /locus_tag="VE308_02495"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZA99872.1"
                     /translation="MAQERAKVPVVSIKFERLKKLVSGIKMNKVLEMLPFVALDIEDV
                     GKDVIRIEYNPNRPDLSSDYGVSRALRGLLDVETGLPKFKLAGRSGLAISIEDRVRKV
                     RPYLIALVAKNGTLDDETLKQLIVMQEDLHNGVGRKRRKASIGIHNLDVIKFPVTYKT
                     VSGDYSFTPLDESSEFTINQILKDLEIGKRYGHILSSFEIYPLIVDSVGTVLSLPPII
                     NGQATKVSDRCRNLFVEVTATDQETAEDLLAILAITLYDAGFEIRTVSINSGRKKIET
                     PQMKPKVISADVGYINSILGLTLDTKQMVTCLKKSRLDAKAVNRKLVCTIPRYRIDIS
                     DPIDISEEVAIGYGIYNLEPKFPPSPTAGEKDSLSVYFNAIREAMIGLGMIESLNFML
                     TSKEVQYSAFGIPAKDVLSVDSSKSTEHEILRDSLVPLLLQSLSYNIHEQYPQRLFEI
                     GKTFLKSTAIIEERWSIGAVIAHTDAGYTEIKSTMQALLRSCFGKEATTKTSTNHMFI
                     GGRCAEVIFDGRSVGILGEIIPLALENFKLRTPVAAFEINLLGIIKDK"
     gene            3885..4925
                     /locus_tag="VE308_02500"
     CDS             3885..4925
                     /locus_tag="VE308_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99873.1"
                     /translation="MSQQDGNKPTEEKKPKAVALLSGGLDSNLAVRMMLDQGIDIEAV
                     AIKTPFCDFDCGKGCGQRVKEVADELGIKLKTVYFGEEYLRMLKNPKYGYGSGMNPCI
                     DCRGMMYNAAKEHMKKINADFVITGEVLFQRPMSQNNRALHIIEKETGMECKVLRPLS
                     AKHLPPTDAEKMGLINRENMGDIKGRSRKGQLTLAKHFGVSEPPNAAGGCLLTDPSFS
                     KRVRDILDHCDNLPTLNDIELLKVGRHFRINHDAKFVVGRNKDENEVIKALVAESDVI
                     MEVKDYVGPTCILRCKNYDDSLVIKCAAIAARYSDAPKDDLSKVSITVNGHESEVTVM
                     PADVETIEMMRI"
     gene            5097..5375
                     /gene="sepF"
                     /locus_tag="VE308_02505"
     CDS             5097..5375
                     /gene="sepF"
                     /locus_tag="VE308_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZA99874.1"
                     /translation="MQTQKTPVYLKAISLRDQSDISVVKEDIKKHMILIIRVTPLAQK
                     DLEELRRVVEDLYSYTQSAGGDIARLGEERIVITPPGVKIWRGIYDLK"
     gene            complement(5367..>5543)
                     /locus_tag="VE308_02510"
     CDS             complement(5367..>5543)
                     /locus_tag="VE308_02510"
                     /inference="COORDINATES: protein motif:HMM:NF019159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HZA99875.1"
                     /translation="HSDYNQLMAYVMRLRPKLRRVLVNHGERPKAQNLASMINKQFRI
                     QTQHPLVQEAIKLL"
BASE COUNT         1731 a         1150 c         1266 g         1396 t
ORIGIN      
        1 cggtcgaaaa tgtgatgcgc ttagcaactt cgatcgcaat tggataaaga tgttgaatgt
       61 gttttgtatt cacaattatc ttagttcttt tcggattgaa accaattgca atgatatcta
      121 agatgttttc gtaggtgtag ctctcaagct ctttcattgt tctttcctga ccgtaaagaa
      181 acttttcgtc atctgtcagt tggaatagca attttgaatc gaatttgtcc tgcaagtatt
      241 ttgtaaagat ccatggcatt agatgcccca tgtggaccat gcccgagggc cccctgccgg
      301 tgtagaggta aaagctctcg cctctgcgct tcttgtctag cagccagtca aagtcacggt
      361 gagagaaaaa ataacctgat tttagcaggg gatggatttc gcctgtcatg ctctttatct
      421 tctgtaatag gtcaggcgtt ataggctgcg tgccaaactt tacgatcagc ttaccatagt
      481 taacttcacc ttcaacttcc cagggtgtga ccttaaagtc agtgctgctg ctctttgagt
      541 cattctgatc ataattgtcg gctaaattca tccctgctgg agcaacagta cattaaacgt
      601 ttaaaaagtc ttacactccc acaactttga ctaatgagtt ctgcaccgac cttccatcca
      661 attgaacgat cgatactcaa ctccctgtcc aataatgatc atgttcgggc cgaatcactt
      721 gttgctagca ctggcctgtc tatagatcag atcagacgag gcatcgagtg gctaaaattc
      781 aagaacctca tttcaattaa agaatcatcg acacaaagag tatttgtggc ctccgaggga
      841 atggccgcag cgaaaaatgg gttgcccgaa agaagattgg taaattctct ggaagaaggc
      901 aagcacacaa ttccagaagt gcttgcgagt ggtgcaataa aaaatgaaga gataaacgcc
      961 gctatagcag gagcaaggcg caaccactgg atccaactcg tccaaggcag gatgattgcc
     1021 actccggata ctaccaggcc ctctcccgaa gagagattca ttgaaaaact aaaaggagga
     1081 atagaaattt caaaactaac agaagaggaa aagcatgtac ttgagcagct gaaaaagcgc
     1141 cctaattaca tagagattaa agaaaaaaaa gaattacgaa tttcacttac cgatggagca
     1201 agaagaataa ttcctaccgt gaatcaagaa atgctagagc gtcgccttac aacagagctt
     1261 attacctcag ggaaatggaa ggagaccaat ttcagctcac ttgatgtaga agcaccttca
     1321 ccaaccattt atccaggaag gcggcaccca ctagttgaca ttattgaaga gatcaaggag
     1381 atctttaccg gtttgggatt ttcagaaata gaaggttcaa taattcaatc aggcttttgg
     1441 aatttcgacg cattatttac tccccaagat catcctgcaa gagagatgca agacactttt
     1501 tacatttcag ctcagatgca gccaatatca gcaagcaaag atcagatttc aaaagtttct
     1561 cgagtccaca atgaaagatg gaatcgccag tgggacattc aagaagccca gcggctagtc
     1621 ctccgtacac acactactcc ggtaactatc cagcatcttg caaagatcaa atcggatgtc
     1681 ggccggttct tctctgtggg cagggttttc agaaatgaaa aggtctcata caaacatctt
     1741 gtcgaatttc accaagtaga gggagttgca acttcagagg aagcatcact ccgtgacctg
     1801 atgggcctgc aaaaagaatt ctatgccaag atgggcatca aacgtatcaa gttttggcct
     1861 acattctttc catacacgga accttcacta cagagtatgg tctacaatga aaaattggaa
     1921 aaatgggttg agctctttgg catgggtatt ttcaggcctg aggtcacaaa gcccctaggt
     1981 atcaaaaacc ctgtacttgc ttggggtggc ggccttgaaa ggatcgctat gttgcgcttt
     2041 ggattggatg atgtacgtga attatatggc aacaagcttg gatggctcag gagcgtgcca
     2101 aagtgccagt tgtaagcatc aaatttgagc gtttgaaaaa actcgtttcg ggtattaaga
     2161 tgaataaggt tcttgagatg ttaccctttg ttgcactcga tattgaagat gtaggtaaag
     2221 acgtcatcag aatcgaatac aatccaaaca gaccagacct atcgtcagat tatggagtgt
     2281 caagagcatt gcgcggtttg cttgatgttg agactggatt gccaaaattt aagttagctg
     2341 gtagaagtgg gctagcaata agcatcgaag acagggtcag gaaggtaagg ccttacctca
     2401 ttgcactggt ggcaaaaaat ggaacgctag atgacgaaac gctaaagcag ttaatcgtaa
     2461 tgcaagaaga ccttcataat ggtgtcggca gaaagcgcag gaaagcatca attggcatcc
     2521 acaaccttga tgtaatcaaa ttcccggtca catataaaac agtaagcggc gactattcat
     2581 tcacgccttt agatgaatct tcagaattca caataaacca gatactaaaa gatttagaga
     2641 ttggcaagcg gtatggtcat attctgtcct catttgaaat ataccctctc atagttgaca
     2701 gtgttggcac agtactttcg ttacctccca taattaatgg ccaagcaacc aaagttagcg
     2761 acaggtgccg caatctattt gtggaagtaa ctgcaactga tcaagaaact gctgaagatc
     2821 tgcttgccat tcttgcaatc acgctctatg atgcaggatt tgaaataaga actgtgagca
     2881 taaactcagg cagaaaaaaa attgaaacac cgcagatgaa gccaaaagta atttctgcag
     2941 atgtgggcta cattaatagc atccttggcc tgacgcttga caccaagcag atggtcacct
     3001 gtcttaagaa aagcaggctg gatgcaaagg cggtaaacag gaaacttgtc tgcactattc
     3061 cgcgatatcg aatagacatc agcgacccga ttgacatctc agaggaagtg gctataggct
     3121 acggcattta caacttagag ccaaagtttc ctccttcacc aactgcgggg gagaaggatt
     3181 ctctttccgt ttattttaac gcaataaggg aggctatgat tggtttgggc atgattgagt
     3241 cactcaactt tatgctaaca agtaaagaag tgcagtacag cgcctttggt attcctgcta
     3301 aggatgtact ctcagtcgac agctctaaga gcacagagca cgaaatcctc cgtgactcgc
     3361 ttgttccgtt gctccttcag tcgctttcct acaacatcca tgaacagtat ccgcagaggt
     3421 tatttgagat aggcaagacc tttctcaaat caactgcaat aatagaagag agatggagca
     3481 tcggtgcagt gatagcacat actgacgcag gttatacaga gataaaatca acaatgcagg
     3541 cactattaag gtcatgcttt ggcaaagagg caaccacaaa aacatcaaca aaccatatgt
     3601 tcataggagg caggtgtgcc gaggtgatat tcgatggtag atccgttggt atacttggag
     3661 agatcattcc acttgcactt gaaaacttca agttaaggac gcctgtggca gcatttgaga
     3721 taaacctgct tgggatcata aaagacaaat agcaccatgt gaaagataat gtaccccgtt
     3781 aaagagcacg tagaggacac agcttaaggc taaccagtga cagcgtgtga gtcagaggcg
     3841 gggcacatat catttttata accaatagac caccaatgcc aacgatgtca cagcaagatg
     3901 gtaacaaacc aacagaagaa aaaaagccaa aggcagttgc attgttatct ggcggcctag
     3961 acagcaacct agctgtaagg atgatgctag atcagggcat cgacatagaa gccgttgcta
     4021 tcaagacacc gttttgcgat tttgactgtg gtaagggctg tggccaacga gttaaagagg
     4081 tggccgacga acttggaatt aagctaaaga cggtatactt tggcgaagag tacctccgta
     4141 tgctaaagaa tcctaaatac ggctatggct cggggatgaa tccttgcata gattgtagag
     4201 gtatgatgta caacgccgcc aaggagcata tgaaaaaaat taatgctgat tttgtaataa
     4261 caggtgaagt gctatttcag cgcccgatga gccaaaacaa ccgcgcatta catatcattg
     4321 aaaaagagac aggtatggaa tgcaaggttt tacgcccgtt atctgcaaag cacctcccac
     4381 cgacagatgc ggaaaagatg ggattgataa accgagaaaa catgggtgat atcaagggta
     4441 ggtcgagaaa agggcagttg acgctggcaa aacactttgg tgtgtccgaa ccaccaaatg
     4501 ccgccggcgg ctgtctatta acagatccca gcttctcaaa gcgcgtcagg gatattttgg
     4561 atcactgcga caaccttcca actcttaatg atattgagct tttgaaggtt ggcagacatt
     4621 tccggattaa ccatgatgcc aagttcgtgg ttggcaggaa taaggatgaa aacgaagtta
     4681 taaaggcact tgtggctgaa agtgacgtca ttatggaggt caaggactat gttggaccaa
     4741 catgtatact acgctgcaaa aactacgatg attctttagt aataaaatgt gctgcaatag
     4801 cggcacgtta ctctgatgca cctaaagacg acctctctaa ggtctcaata accgttaacg
     4861 gacacgagag tgaagttaca gtcatgccag ctgacgttga gacgatagag atgatgcgta
     4921 tataaacaaa aataataatg ataataatta taataatatc acatcctata aagagaagaa
     4981 actctctatt accatcattc agctaaaatc aactattcgg taaatgagaa ttgaccagca
     5041 taactattga ttacacattg caagctcaac tgcggttcgc agaagataga tagcagatgc
     5101 agacacagaa gacaccggtt tatttgaagg caatttcgct gcgagatcag tcggacatct
     5161 ctgttgtaaa ggaagatatc aaaaagcata tgatcctgat tatcagagtg acaccgcttg
     5221 cgcaaaaaga cttggaggaa ttgcgcaggg tagtggagga tctctattcc tatacacagt
     5281 cagcgggcgg cgacatagca agacttggag aagagcgcat tgtgatcact cctcccggcg
     5341 taaagatctg gcgtggcata tatgatctaa agtagcttta ttgcttcctg caccagcgga
     5401 tgctgtgtct gtattctaaa ctgcttattt atcatactcg ctaggttctg cgcctttgga
     5461 cgttcaccgt ggtttacaag gactcgccta agcttgggac gaagtcgcat aacatatgcc
     5521 ataagctggt tataatcact gtg
//
LOCUS       DASQTF010000105         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1324825,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000105 DASQTF010000000
VERSION     DASQTF010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324825"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VE308_02515"
     CDS             <1..265
                     /locus_tag="VE308_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HZA99876.1"
                     /translation="VAIQGSGWGWLVYNQNTNGVQFITMPNQTSPWTKWKTEKVTPLL
                     GLDVWEHAYYLKYQNRRADYVSAWWNVVNWDEVKRNLQQALQR"
     gene            complement(481..1215)
                     /locus_tag="VE308_02520"
     CDS             complement(481..1215)
                     /locus_tag="VE308_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZA99877.1"
                     /translation="MQHDNNPVKDALDLLSKKWKIDPKLYDFQSGKYKEVVSDTSLNV
                     GGINYHIPTLSKDGLYILWKCLWPDCHNCCERQGRLPLTKDDIGRISKKVGYNSKAEF
                     VRNETTISSWQEQEAFGNVITTLSMLSLKRKKDEKPEEDGTPLRCRFLNEEGYCGIHP
                     DKPGVCWLYPFASWIEVDARGQPAVHATFQFTGDCPGFYLDGSIDSMMPVLQEYSTKI
                     YDYNMAISRTTREGYGFLNFVDLRKS"
     gene            1287..1652
                     /locus_tag="VE308_02525"
     CDS             1287..1652
                     /locus_tag="VE308_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99878.1"
                     /translation="MKEQQQEDEEEEAAVIRIKGKHASSTENRRMVWENVVWPLVLEM
                     NRSYFTLKEYHAKRNAFCRKTGVSPSKVAGGFVSLLVKGIIVRDKNMYSIHYRLIPYM
                     RKKAELEYGLVTREIYTKR"
     gene            1692..1837
                     /locus_tag="VE308_02530"
     tRNA            join(1692..1701,1728..1775,1800..1837)
                     /locus_tag="VE308_02530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1772..1774,aa:Gln,seq:ctg)
     gene            complement(1846..3393)
                     /gene="pckA"
                     /locus_tag="VE308_02535"
     CDS             complement(1846..3393)
                     /gene="pckA"
                     /locus_tag="VE308_02535"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZA99879.1"
                     /translation="MDITFGRAYNNLSVPSLVEIIIKRKEGILSSTGALSVKTGKFTG
                     RSPDDRYIVDDEETHNTIDWGKVNHPISEASFEKIFRMMRKHVEDKEFFIFDGFVGAD
                     PENRLPIRIVNNRAWHNLFARQLFIRPKPEELENQVPRFTLLSCDDFAAKPEEMGTRT
                     ETFIIINFKKKLVLIGSTSYAGEIKKAMFSIMNFILPKKGVFPMHCSANVGKKGDTAL
                     FFGLSGTGKTTLSADSDRRLVGDDEHGWSDNGIFNFEGGCYAKCINLEKESEPQIWSA
                     IRFGSVMENVVMHNGTREPDFDNGSLTENTRVAYPIDYINGAVLPSTAGHPKVIVFLT
                     ADAFGVMPPIAKLTKQGAMYHFMSGYTSKLAGTERGITEPKETFSHCFGAPFMPLHAS
                     KYAEMLGKKITEHGTRVYLINTGWTGGPYGIGKRMNLTYTRAMVTAAIKGKIEDVTVK
                     HHEIFNLDMPTSCIGVPDEVLNPRNTWSDREKYDATARRLAALFVKNFEKFGNIPKEI
                     VKAGPRI"
BASE COUNT         1006 a          880 c          842 g         1136 t
ORIGIN      
        1 tgtcgcaata cagggaagcg gctggggctg gctcgtatac aaccagaata caaatggcgt
       61 ccagttcata accatgccta accagacaag cccttggaca aagtggaaga cagaaaaagt
      121 caccccgctt ttgggtcttg acgtatggga acatgcctat tatctcaagt accaaaaccg
      181 ccgagcggac tatgtatctg cttggtggaa tgtggttaac tgggacgaag taaaaagaaa
      241 ccttcaacaa gcccttcaaa ggtaattcta ccccttttta tatttttttc aaccattttg
      301 aaattattag acggcaaaat catgctgcta caatattagc tgatgccata gtatttggtt
      361 atatgtctct gcctgcgatt cagcctttat agaatatgaa aagtgcggca acccattttt
      421 ggtggtatat ccacacgccg gctctctgta gtgaatatac agaattctgc gcaggagctg
      481 ctaactctta cgcagatcta caaaatttaa aaacccatag ccctcgcggg tagttctgct
      541 tattgccata ttatagtcat aaattttagt agagtattct tgtaatactg gcatcatgct
      601 gtctatcgat ccgtcaaggt aaaagccggg acagtctccg gtaaattgaa aggtggcatg
      661 tacagcaggc tgccctctcg catcaacttc aatccacgat gcaaatggat agagccagca
      721 gacacccggc ttgtctgggt gaatgccgca atagccttct tcgtttagga acctgcagcg
      781 aagcggtgtc ccatcctcct caggcttttc atcttttttt cttttgagcg agagcatact
      841 caacgttgta ataacatttc caaacgcttc ctgctcttgc caactggaga tcgttgtctc
      901 gttcctaaca aattcggcct tcgagttata gccaaccttt tttgatattc tgccgatgtc
      961 atcctttgtc aatggcaatc tgccctgccg ttcgcagcag ttgtggcagt caggccatag
     1021 gcacttccac aagatgtaca ggccatcctt tgacaaggtt gggatgtggt aattgatccc
     1081 tcctacatta agggaggtgt cagatactac ttccttgtac tttccactct ggaaatcata
     1141 caacttggga tctattttcc attttttgga aagaaggtca agtgcatcct tgaccggatt
     1201 gttatcatgc tgcactatgt tatagggtta taaaaccggg ggtttaaacg tactctcaat
     1261 tgctcagcca tcggcagcca caacccatga aagagcagca gcaggaggat gaggaggagg
     1321 aggcagcggt tatcagaata aagggcaagc atgcatcttc tactgagaac cgcaggatgg
     1381 tctgggaaaa tgtcgtttgg ccccttgtct tagagatgaa caggtcttac tttacattaa
     1441 aggaatacca cgcaaaaaga aatgcatttt gcaggaaaac cggtgtctcg ccttcaaaag
     1501 tcgctggtgg ttttgtgtca ttactggtaa aaggcatcat cgtcagagac aagaatatgt
     1561 actctataca ctaccgtctc atcccctaca tgaggaaaaa agcggagctt gaatacggtc
     1621 ttgtcactag ggagatctat actaagcgtt aaaatatccg atcaaagagc cggcttgatg
     1681 tagctcgtgt tagcccggtc gaatccaata aaaaattgga tgccgaatct agtggccaat
     1741 ctccaaggtt gagggctagg gattccaggc tctggatctc acaaagatag agaaaagaaa
     1801 cctggagaca gcagttcgaa tctgctccgg gctaccatct tttttctaaa ttcttggacc
     1861 tgctttcact atctcttttg gtatgttgcc aaacttttca aagttcttga caaagagcgc
     1921 cgcaagtcgc cttgctgtgg catcatactt ctccctgtca gaccaagtgt tacgtgggtt
     1981 aagcacttca tcaggtacgc caatacatga tgtgggcatg tcaaggttga atatttcatg
     2041 atgcttcaca gtcacatcct cgatcttgcc tttgattgct gcagtgacca tcgcccttgt
     2101 gtatgtcaga ttcattctct tgcctatgcc atatggacca cctgtccacc cagtattgat
     2161 caggtatact cttgttccgt gctctgttat ttttttgcca agcatctcgg cgtacttgga
     2221 tgcatgcagt ggcatgaatg gagctccaaa gcagtgcgaa aatgtctcct ttggttcagt
     2281 gatgccgcgt tcagttccgg ccagcttgct cgtataacca gacataaagt gatacattgc
     2341 gccttgcttt gtcagcttgg ctattggggg catgactcca aaagcatctg ccgtaaggaa
     2401 tacaattact tttggatggc ctgcagtact tggcagcacc gcgccattga tatagtcaat
     2461 aggatatgcg acgcgtgtat tttcggtaag acttccattg tcaaaatctg gttcccttgt
     2521 gccgttatgc attacaacat tttccatgac tgatccaaaa cgtattgcgc tccaaatctg
     2581 cggctcgctc tccttttcaa ggttgatgca tttggcataa cagccgcctt caaagttgaa
     2641 aatgccattg tcagaccaac cgtgctcgtc gtcgcctacc agcctgcgat ctgaatcggc
     2701 tgaaagtgtg gtcttgccag tgcctgatag gccaaagaat aaagcagtat cacccttctt
     2761 tcctacatta gcagagcagt gcataggaaa tacgcctttc tttggcagga taaagttcat
     2821 aatggaaaac attgctttct tgatctcacc agcatacgaa gtcgacccaa tcaggaccaa
     2881 ctttttcttg aagttgatga tgataaaggt ctcagttctt gtgcccattt cttccggctt
     2941 tgcggcaaag tcgtcacatg agagcaaagt gaagcggggt acttgattct ctaattcctc
     3001 tggcttgggc ctaatgaata gctgccgggc aaagagatta tgccaagccc tgttgttgac
     3061 gatccttatg ggaagcctgt tctccggatc tgcccccaca aagccatcaa agataaagaa
     3121 ctccttatct tctacgtgtt tcctcatcat acgaaatatc ttttcgaagc tcgcctcaga
     3181 aattgggtgg ttaactttcc cccagtcgat ggtattgtgt gtttcttcat catcgactat
     3241 atacctgtca tctggcgacc tgccagtgaa tttgccagtt ttgacagaaa gagcgccagt
     3301 tgacgaaagg atgccttctt ttcttttgat tatgatctcg accagcgacg gaaccgacaa
     3361 gttgttgtag gctctgccga acgtgatatc catcttgcaa gctgtatcat agactttgta
     3421 gttataatct ttaggttgtc ttcggtaagc aaacatacta ctaatattct atgtgactac
     3481 agggtcttgt gtaataacat agaagcgaat tccaattttc tctctatacc taattctcct
     3541 gcatcgtctt tacagtctct aataggtcat attttttctt ctttattgta agaagagaga
     3601 tatttcatag cgcgacttaa gcatgcaaat taagcgaaga tttggtcaga ttttgtacgt
     3661 atggtatgac cattctccag gcagtagaca atcatatttc tacaggcaga gagggtcatt
     3721 caaaaaaaga gggacatcag ctataggtct atcttttcac ttttgggtaa ttgttagctt
     3781 tcagatatca ccaaaatgta tgcgtttcag gagtgctatt gctgttgctg ctgttttaat
     3841 gcgttcctaa tcgcgtcagc tctt
//
LOCUS       DASQTF010000106         5855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_111186,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000106 DASQTF010000000
VERSION     DASQTF010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111186"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..295)
                     /locus_tag="VE308_02540"
     tRNA            complement(221..295)
                     /locus_tag="VE308_02540"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Val,seq:tac)
     gene            395..1822
                     /locus_tag="VE308_02545"
     CDS             395..1822
                     /locus_tag="VE308_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99880.1"
                     /translation="MKKLPRVESTKIPFLVLIVVAFFAALILFTPVFPSAFAHGGHQP
                     PAADFEGKKASLFVKLDPPVVTDISQPIFISARFFDENSNQNFEEVTYRIFFQKDGTE
                     IPIVTEGGQFGGQGFFYDPEGDLQIRVVPRDTETVVARGEAEPQFGGIWNRGGPIVVE
                     GPIFIEPGLYNLFIEVHTVGTTRTQVDPALRYDVWVTPGREEAINISEGGQTQQVKIR
                     NYYGAIDSSNYDPGTKTIQFSMPFDWTSDMIGRIGMLHTEVFIPKALSDFNKQSLNAT
                     VNGISVPVAVDTYTPEATIVHYTISKTNLENIASKVTSENRTPDKAVFALGPPDPDSE
                     VKVAQVSAESENYRVGLTWPEQMLPQQPVTFGIRITDKSDTPVYAAAYEFALVDKDGN
                     EITRSGGVTTPEGLSSQDVTFASPGSFNVRIEKIKDTNESVQSGLTVVPEFPIGIASI
                     VATIAIAAIIIATKKMSFLQMGKMY"
     gene            1845..2144
                     /locus_tag="VE308_02550"
     CDS             1845..2144
                     /locus_tag="VE308_02550"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZA99881.1"
                     /translation="MSDDEYRKLSQSEIEHEVGKLDNWNVVNGKINKTFEFDDFVQAF
                     GFMTKVAMNAEKMNHHPEWFNVYNRVRIDLVTHDVNGISNYDIALARIIDTIAKV"
     gene            2459..2710
                     /locus_tag="VE308_02555"
     CDS             2459..2710
                     /locus_tag="VE308_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99882.1"
                     /translation="MVSSPFVELADKKGYWINELESLGISPKEIERDYESESECIKEL
                     ADHVENIKQALYQEYKTSYSERMMQLEGEEEEEENAIVI"
     gene            complement(2991..3383)
                     /gene="hsp14"
                     /locus_tag="VE308_02560"
     CDS             complement(2991..3383)
                     /gene="hsp14"
                     /locus_tag="VE308_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp14"
                     /protein_id="HZA99883.1"
                     /translation="MTLIHYAARELSREIGKRSREFYEFVMPAIDMYEDENGLIVKID
                     LPGFKRDEIKISVEEDILSIRAKRMEPQDESLGTILYKQRPIQIDKRISLPISIKEGE
                     KVTGTATYSDGVVTLRIPISRVGVIPVT"
     gene            complement(4147..4668)
                     /locus_tag="VE308_02565"
     CDS             complement(4147..4668)
                     /locus_tag="VE308_02565"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZA99884.1"
                     /translation="MRNVKVIQSSMIYFGGINLRKDKFSLNGRVVDKEIVGHQDSVGI
                     VAVRGSSVILVTQYRHAANKMLLEIPAGKIEKGETPRQAAVREMAEEIGCSGTLRPLL
                     KWYLAPGYSTELMHVFVATNLREITRGLLDNDENIEIKKMRLSAAIKKCLNGNIEDAK
                     TIAALLAYYSQSN"
     gene            4741..5160
                     /locus_tag="VE308_02570"
     CDS             4741..5160
                     /locus_tag="VE308_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99885.1"
                     /translation="MEFIDGDGNIIVPKAVRPIIDLKETPLSNKKGANKQYRCGSFHI
                     RDYDTHYGVHMDTVDPLSNPLGYLLADAPEYLAGTTAAFIVGRRLGITVYIICKKEGK
                     STKDAAIGAVLAGYIAGSAAGNLVFNMASSLKKKRGE"
     gene            5166..>5855
                     /locus_tag="VE308_02575"
     CDS             5166..>5855
                     /locus_tag="VE308_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HZA99886.1"
                     /translation="MESPAQIRKEDTTSVKEASVFPHPSKLHVAKVVLKLLPLVINLR
                     RDRREWVKREGKNVDEEKYRRHARKVLKTFIDLGPSYIKLGQWLSTRVDILPQPYLEV
                     LSKLQDDVPPALFSQVKPVIENELGRIEDAFEEFDTAPLSGASLGQVYLAKYDGKQVI
                     VKVSRPNIERVIEDDIYVMKKVLPLATRFIDPNLRFSAEGMMAQFIETVHEEMDYRIE
                     AENLLAIERNLA"
BASE COUNT         1687 a         1251 c         1310 g         1607 t
ORIGIN      
        1 tgtttgcccg catccattct ccaaacgaat ttgcaaggat tgacctattg aacaagatga
       61 cgaagtgtat tggaagagtg atggaaaagt ttgccagcta gaggatagat tgctaaatta
      121 ataatcatgt gagcagctct attcgtgtct tgaatcgtgg acactatgtt ctggagtgtc
      181 tctgctatgt acgtcgggaa agactcagag cactaaagaa tggatgggga gagatttgaa
      241 ctctcgacca cctgtgtgta agacaggtat cctaaccagg ctagactacc catccagcaa
      301 aactgctcgt cagttgagcg gatcggtatt aatagtattt ggagtcacat gctcgtgaaa
      361 ataaccaata tataccctgc tataattttg atttgtgaaa aaattgcctc gagttgagag
      421 tacaaaaatt ccctttcttg ttttgatagt tgttgcattt tttgctgccc tgattctttt
      481 cactcctgtt tttcccagcg cgtttgctca tggcgggcac cagccgcctg cagccgattt
      541 tgaaggcaag aaggccagcc tctttgtcaa gcttgatccg ccagtagtaa ctgatatcag
      601 ccagcctatc ttcataagtg caaggttctt tgacgaaaac tcaaatcaaa attttgaaga
      661 agtgacgtat aggatctttt ttcaaaaaga cggcactgaa atcccaattg taacagaagg
      721 tggccaattt ggcgggcagg gcttcttcta tgatcctgaa ggcgacctac aaatacgggt
      781 ggttcctcgg gacactgaga cggtagtagc tagaggcgag gcagagcccc aatttggcgg
      841 gatctggaac aggggagggc ccattgtggt agaggggcct attttcatag agcctggcct
      901 ctataacctg tttattgagg tgcatactgt tggcactacg agaactcagg tggaccctgc
      961 gctcagatat gatgtatggg tgactcctgg ccgagaagaa gcgatcaaca tctcggaagg
     1021 cggccagact cagcaggtca agatcaggaa ctattacggg gccattgaca gctctaacta
     1081 cgatccaggg acaaagacaa tccagttttc aatgcccttt gactggacgt ctgatatgat
     1141 aggcagaata ggtatgctcc atacggaggt attcatacca aaggcactat ctgactttaa
     1201 caaacaatcg ctcaatgcta cagtaaatgg cataagtgtc cctgtggcgg tagataccta
     1261 caccccagag gccactattg tccactatac catctcaaag acgaaccttg aaaacatcgc
     1321 tagcaaggtg acgtctgaga ataggacacc ggacaaggcc gtgtttgcgc tgggacctcc
     1381 agaccctgat agcgaagtca aggtggctca ggtctctgcc gagtcagaga attatagggt
     1441 aggtctcaca tggccggaac agatgctccc ccagcaacca gtaacgtttg gcatcagaat
     1501 aactgacaag tctgacacgc cggtgtatgc tgcagcatac gaatttgccc ttgtggacaa
     1561 ggatggcaac gaaataacac gctccggagg agtgacgact cccgagggcc tatcttctca
     1621 ggatgtgacg tttgcatcac cggggtcatt caatgtgcgg atagaaaaga taaaggatac
     1681 gaacgaatcg gtgcaatcag gcctcactgt ggtgcccgag ttcccaatag gtatagcttc
     1741 tatagtggcc acaattgcaa tagctgctat aataatagct accaagaaga tgtcattcct
     1801 tcagatgggc aagatgtatt agatacatcg atccacttaa ttttatgtca gatgatgagt
     1861 atcgcaaatt gtcgcagagt gaaatagagc atgaggtagg caagcttgat aactggaatg
     1921 ttgtcaatgg caagataaac aagacatttg agtttgacga ctttgtgcaa gcatttggat
     1981 ttatgacaaa agttgcaatg aatgcagaaa agatgaacca ccatcctgaa tggtttaacg
     2041 tttacaaccg cgttaggatc gaccttgtaa cgcatgacgt caatggcata agtaactacg
     2101 atatcgcact tgcaaggatc attgacacta ttgccaaagt ctaaatgtat gcgttaggat
     2161 taagcagatg ctgctattgt tgttttcttg catcacatta ttctctgcta cgcttgtgtc
     2221 atgcaacatg gcagccatga tggggatgaa accttatgtt gcaatgctga gcatagatct
     2281 taaaagaaag cgtattcttg catcaccgta agtgctcaag gcggagctac tcaatccatt
     2341 tgcgatctga cttctttcgc tactgctcct cgctataata taatagtcat tcttgaccct
     2401 cgcgggattc ttatattgtg agagtaatag atctctctgc acggtgagat aaaggaacat
     2461 ggtatcttcc ccatttgttg aactggcaga taagaaaggc tattggatta atgaattgga
     2521 atcccttggc atatccccaa aagagattga aagggattat gaaagtgaat ctgaatgtat
     2581 caaagagctt gccgatcatg tggaaaatat caaacaggca ttataccaag aatacaaaac
     2641 ttcctatagc gaaaggatga tgcagctaga aggagaagaa gaagaagaag aaaacgccat
     2701 cgttatttaa cagaaaacaa atatcaaaat ttcagaaaag gtcagactgt cattatgtcc
     2761 cagagttaag caatgataca tgtattagag actagcatat cttgaggcag tcataggatc
     2821 ttcacgtcgg aaacctcaag atacagagta atccttgtcc cagttggaat tcaggcagca
     2881 agatcatacc agctatacta aacatcaata gcttaaggta agacaaatag taagcagagg
     2941 tcaaataaga aagatcaata atctgtttga tccgttaatt tagttcgtta tcatgttacc
     3001 ggaataacgc ctacccttga aataggaatc ctcaatgtaa caactccatc agaatatgtt
     3061 gctgttccag tcactttttc tccctcttta atagagatag gcaacgatat tcttttatct
     3121 atttgaatag gtctctgttt atagagtatg gtgccaagac tttcatcctg tggttccatt
     3181 cttttcgctc ttattgacag gatatcttct tcaacactaa tcttgatttc atctcttttg
     3241 aaacccggta agtcaatctt aacaatcaga ccattttcat cttcatacat atctattgct
     3301 ggcattacaa attcgtaaaa ctcacgtgat ctcttgccta tttctcgaga taactctcga
     3361 gctgcataat ggattaaggt catgcaacat tagattttgt gttcattgta taaatatttt
     3421 ttatcagcat ttaccttgga ggcaattcct tagtgacatc aagcaacagg aagtcgttac
     3481 aataaggtcc ttagtcttag tgcaataccc ttcatcaacc ggaagcatct ctctatattt
     3541 tccattgtga tacttcggtt gtctctgttt atgtttttag ggtctttgac tactcctaac
     3601 cacatacgat tataaggttt agcgcttatt cctatgttat ttactatatt caactaacaa
     3661 caacaaggct gtatgacgtc aacagaaagt gaagcaagcc ctgtagcaga aattgttgat
     3721 tctgtcacgt gactttattg agcaaagaca aaaggtggcg catacattcc ctgattagtt
     3781 aaatttcaac tgcattgcac tgaattcgct cggatttcat ctctgcaaaa ctaacaagta
     3841 ttactagtac cttgtttagc aatttgtact tggttacctt taaatggaat gacactattt
     3901 cgtgctacta ttcgctgatt tccatttgct gtgaagccat ctatacgctc gctcatctaa
     3961 tgaatatagc caattatatt tcggtagtca ggtggcatgg gaggcgaaag acaggccgag
     4021 cttatcataa tctgccacat ggtagggagg actgtgcaag tagcaatcga caacttgaac
     4081 cgtcagggtt tggtgattga aaggaatagt ctagatcacg taatcaaaaa aggtttaatc
     4141 gccgattcag ttggactgac tgtaatatgc tagcaatgca gcaatggtct ttgcgtcctc
     4201 aatatttcca ttgaggcatt tcttaattgc tgcactcaat ctcatcttct ttatctcaat
     4261 attctcgtca ttatctagaa ggcctcttgt tatttctctc aggttcgtag caacaaatac
     4321 atgcatcaac tcagtagagt aaccgggcgc aagataccat ttcaatagcg gtctgagagt
     4381 gcctgagcag ccgatctctt ctgccatttc gcgaactgcc gcctgcctcg gcgtctcacc
     4441 cttttctatc tttcctgcag ggatctctag cagcatcttg tttgccgcgt ggcggtactg
     4501 ggtaacgaga ataacgctgc tacctctgac ggctactatc ccaacagagt cttgatgtcc
     4561 aacaatttcc ttatcaacaa ccctgccatt tagtgagaat ttgtctttcc tgagattgat
     4621 gccgccgaag tagatcatgc tgctttgaat gactttaacg tttctcatcg tcttacagat
     4681 gtgaggcgag gattaaacct attcgttaat tttatatcca attggcatct tttttgaaac
     4741 gtggagttca ttgacggcga tggcaacatc attgtaccca aggccgtcag gcctataatt
     4801 gatctcaagg agactcctct gagcaacaag aagggagcca acaagcagta ccgctgtggc
     4861 agctttcaca tcagagatta tgatactcac tacggtgtcc acatggacac agtggaccca
     4921 ttaagtaacc cacttggata tctgcttgcg gatgcaccgg agtacttggc aggaacaaca
     4981 gctgcattca tagtcggcag acgattaggc ataacggtct acattatatg caaaaaggag
     5041 ggcaagagca caaaggacgc tgccatcggc gcggttctgg ctggatacat cgccggctct
     5101 gcggcaggca accttgtttt caacatggcc agctcgctga aaaagaaaag gggtgagtaa
     5161 gctcagtgga gagcccagcc cagatcagaa aagaggacac tacttctgtc aaagaagcat
     5221 cagtctttcc gcacccttcg aaattgcacg tcgctaaggt ggtgctcaag cttttgcccc
     5281 tcgtaataaa tttgcggcgc gacaggcgcg aatgggtcaa gcgtgagggc aagaatgtcg
     5341 acgaagaaaa gtaccgcagg catgctcgta aggtgctcaa aacgttcatt gatcttggac
     5401 catcatacat caaacttggt cagtggcttt caacccgggt agacattttg ccacaaccct
     5461 accttgaagt gctctcaaaa ttgcaggatg atgtcccccc tgctctgttc tcacaggtaa
     5521 agcctgtaat tgaaaatgag ctaggcagga tagaggatgc atttgaagag tttgatactg
     5581 cccctctgtc tggagcaagc cttggacagg tctacttggc aaagtatgat ggtaagcagg
     5641 ttatagtcaa ggtgtcaagg ccaaatattg aaagagtaat tgaagacgac atttacgtta
     5701 tgaaaaaagt attacccctt gcaacacggt ttatcgatcc gaatttgaga ttctcggcag
     5761 aaggcatgat ggcacagttc attgaaacgg tacacgaaga gatggactac cgcatagagg
     5821 cggaaaacct gcttgcgatt gagcgcaatc tggca
//
LOCUS       DASQTF010000107         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1042827,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000107 DASQTF010000000
VERSION     DASQTF010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042827"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VE308_02580"
     CDS             <1..1072
                     /locus_tag="VE308_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HZA99887.1"
                     /translation="NLTDLKPKELIIRPTVAVSSLAEFLSKLKKKKNNLIVNVDPKLA
                     SASVRTIHESEEADMVICTTFDQLKSFKSSGKSVAYFKKVTSNLDVDEIERASENGAE
                     VVIVDASNWKIIPLENIIAKLHKSKTKVFATAKSSEEVATLFAVLELGVDGVILSTDS
                     EEEVDRARQQLTSARFLITRARIIEVKDVGTGERVCVDTASMMRMGEGMLVGSRSNFM
                     LLIHNESIGSSFTSPRPFRVNAGAVYCYTIVPGGSTRYLSEIESGSEVWIVNKEGIAR
                     SAVVGRSKIETRPLRLIRAEINGEKGTVILQNAETIRLISKDGKLLSVTEVKVGDEVL
                     GFAKQPGGRHFGMEVDEFIVEK"
     gene            1374..1520
                     /locus_tag="VE308_02585"
     CDS             1374..1520
                     /locus_tag="VE308_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99888.1"
                     /translation="MASNIEDASDKIKAGAKATASKASDTDRDMDREYDKEMYKEKFK
                     EKMD"
BASE COUNT          696 a          393 c          493 g          572 t
ORIGIN      
        1 gaacttgact gatctcaaac caaaagagct aataatcagg ccaactgtag cagtatcttc
       61 tcttgcagaa tttctctcaa aactaaagaa gaagaagaac aatttgatag tcaacgttga
      121 tcccaagcta gccagtgcca gcgtccgtac aatacatgag tcggaggagg ctgacatggt
      181 gatctgcacg acttttgatc aattgaaatc attcaagtcg tcagggaaga gtgttgcata
      241 cttcaagaaa gtgactagta accttgacgt cgatgagatt gaaagagcat cagagaatgg
      301 agcagaagtt gtaatcgttg atgcatccaa ctggaaaata atcccactag aaaatatcat
      361 tgccaagcta cataagtcta aaacaaaagt gttcgccact gcaaaaagct ctgaagaagt
      421 tgcgacattg tttgcagtcc ttgagctagg agtcgatgga gtaattttat ctacagatag
      481 cgaagaggaa gttgacagag cgagacaaca gctaacttct gcgaggttcc tcatcacgcg
      541 agctaggatc atagaggtaa aagacgtcgg cactggcgag cgggtgtgtg tagatactgc
      601 ctccatgatg aggatgggag aaggtatgtt ggttggaagc agatctaact ttatgctttt
      661 aatccacaat gaatcaatcg gatcttcttt cacttcgccc aggccattcc gggtgaatgc
      721 cggtgcagta tattgttaca caatagtgcc tggtgggtca acgcgctatc tctcagagat
      781 cgaatcagga agtgaggtat ggatcgttaa taaggaaggc attgcaagaa gtgctgtcgt
      841 cggcagatca aagatagaga cgcgaccgct aagactaata cgcgcggaaa tcaatggtga
      901 gaaaggcacc gttattttac aaaatgctga gacgatacgg ctaatatcca aggatggcaa
      961 gctgttatca gtgactgaag taaaagtggg cgatgaggta ctgggttttg ccaagcaacc
     1021 cgggggtcga cacttcggga tggaagttga cgaatttatt gtcgagaaat aatattacta
     1081 gtattcttca aggatgactt tttgttttat ccataattta ataaaatgat atactttaat
     1141 tcgaaggaac ttatgttatt agctatagtg ggcgcttgat atcttaaggc gaaaagaaag
     1201 gtatacacac actagcagat ctatatgggt tctggaaccg aaaaatgcaa ctttaacaag
     1261 ccaggagcaa taactacacg gtcgataata tctttccgga agaggcaagg aagattagac
     1321 tgaatcttgt gagtaacctc ttaaataatt tgatgttata tatctaaggc accatggctt
     1381 caaatatcga ggacgcatct gacaaaatta aggctggtgc aaaggctaca gccagtaagg
     1441 cgtcagatac cgatagagac atggatcgag aatacgacaa ggagatgtac aaggagaagt
     1501 tcaaggaaaa gatggactag gacttggatt tgggctccgg ttttagggtt catctatcac
     1561 cttctccttt cttttccttg cattaattaa gaattcgcta aatagctgta acttttctca
     1621 attcaatgtt aattcatgtg ctgataatga aaaaggagca acatattcct aacgtgatat
     1681 ccctcttaaa cggaaacgtt gtggccgtct ctcaacagct atattcattg ttggattctc
     1741 aagcagaaaa gttacactta tgcagtagag cagtattaac tgaggataca tagcaaacac
     1801 tttagctaga aattggcaaa cgcaaatgaa taagagtgtt atgtcttctg cataaaacag
     1861 atgtatatac attgggtatg aaaggtttcg acgacgaaag agatcagaca aactgctaac
     1921 tgacatatcc acctttgaca tagatccaaa tcgatactta gcaaaaaatt ggtaggcttc
     1981 tcattcttct agctcatttt tgcaagctga aggcatagct tctttttagt ccgttgttta
     2041 ttactttaaa gtatggggtc tttggacgaa tcaccaaagc atgctgacag tatcaaatca
     2101 tgcttgtcca acaagaaatc gcaaaccaaa tagctgtcgg caattgtagg gacg
//
LOCUS       DASQTF010000108         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1224214,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000108 DASQTF010000000
VERSION     DASQTF010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224214"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VE308_02590"
     CDS             <1..1042
                     /locus_tag="VE308_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZA99889.1"
                     /translation="PEKIGTYFGEQKDIREITISTYQSAVANLDIVKRADMIILDEVH
                     LVSATARSFSRIFDIISNGSKALLGLTATIDEVDPKNQIIMAIIPPARKYLIKDAVTD
                     GRLARPIINPVKVNLTEQEQRTYNEFSRKIRTISSRFKRYDAKAMIELAKQGGFPSWQ
                     AKAWFLNVRKRKGLLASAENKLAMAVELIKTKHPNQKVMVFSETLESIRKLKQQLKSK
                     GVEAMLIDSRMPSFRRQKVLSQWGKRFYPLLSVHTLEIGYDVPEVAVEIILATTSNMN
                     QIVQRVGRVLRKVEGKESALVYVIYVSDSKDDNTLACVKSAIDTAADGIFPHSWAGSR
                     NRPQPIGTNSNL"
     gene            complement(1231..>2287)
                     /locus_tag="VE308_02595"
     CDS             complement(1231..>2287)
                     /locus_tag="VE308_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99890.1"
                     /translation="IIADILFSAATDHRYLDSGHVLDFTNKAFEALDVVGWNLAEQVL
                     TSLVTLYAQATRMEERSSWRHPIDIIALLNDCFDKLPAVLEKSKQSRTTWKASKKIVD
                     VLLGDSPNAIAEVLIESLQDGAKQEELAAIVAYAAALRIAQFPITNEYSDWDTALHTF
                     TFANAVQQAIRRLPSSMELLRAVFDVAMSNYLNRFLNVPSVAIPSEKMEPINKNKNNK
                     NNNNNKDNAKNGVDIRETFLDTLDKRQQVNEAAKMVASCLYTQGEKALSAILVQALLR
                     EDRSFHTIQMVEAAFRQKTEVQRLQVLDDIKPISHILIAAARYLAAHTPTARAQGQTF
                     EIAWRLNKDGKLYEEID"
BASE COUNT          683 a          473 c          491 g          640 t
ORIGIN      
        1 accggagaag ataggcacgt attttgggga gcaaaaggat attcgcgaga taacaatcag
       61 tacctatcag agtgccgtag ccaacctaga cattgtaaaa agagccgata tgataattct
      121 tgatgaggtc caccttgtga gcgcaacagc acggtcattt agcaggatat ttgatattat
      181 ctcgaatggc agcaaggcgc ttttgggact aacagcaact attgatgaag ttgatccgaa
      241 gaaccaaata atcatggcaa tcattccgcc agctagaaaa taccttatca aagatgcagt
      301 cacagacgga cgcttggcaa ggccaattat caatccagta aaagtgaacc tgacagagca
      361 ggagcaaaga acctataacg aatttagcag gaaaataagg actatctctt ctcgcttcaa
      421 aagatacgac gctaaggcaa tgatagagct agcaaaacag ggaggctttc caagctggca
      481 ggcaaaggct tggttcctaa acgttaggaa aagaaaagga ctactcgctt cagcagagaa
      541 caagctggca atggctgtag aactgataaa gacaaagcac cccaaccaaa aggttatggt
      601 ttttagcgaa acgcttgaat ctatacgcaa actaaagcag cagcttaagt ctaaaggcgt
      661 tgaggcaatg ttaatcgaca gcaggatgcc atcatttagg aggcaaaagg tactatcaca
      721 atggggaaaa aggttctacc ctttgctttc tgtccatacg cttgaaattg gttatgacgt
      781 tcctgaagtc gcagtagaga taattctcgc aacaacttct aacatgaacc agatagttca
      841 gagggtagga agagtgctac gaaaagttga gggaaaagag agtgcattag tatatgtaat
      901 atatgtgtca gatagcaagg atgataatac tttggcatgt gtgaaaagcg caatagatac
      961 agcagcagat ggaatatttc cgcattcttg ggctggtagc cggaatcgtc cccaacccat
     1021 tggtactaat agcaatctgt aactaataca gacacaccct atagtgcgac cgaatattat
     1081 ttttcatcct gtgcttctga ttcatcaagt ctgttttctg aaggtttttt cctcggatcc
     1141 ctggtcttct agctttttct ggttacaatt catctggtgt tatatatcga tcctaggaag
     1201 aaagccgtaa aaaagaatta aacacgatta tcaatcaatt tcttcataca gctttccatc
     1261 tttgttaagc cgccacgcaa tttcaaatgt ttgtccttgt gcacgcgcag tcggtgtatg
     1321 agcagccaag tagcgtgcag cagctatcaa gatatgcgaa ataggtttga tgtcgtctaa
     1381 gacttgaagg cgctgaacct cagtcttctg cctaaaagcg gcttctacca tctggattgt
     1441 gtggaagctt ctatcctctc ttaacagggc ctgtacaaga attgcagata gtgctttttc
     1501 tccttgcgta taaaggcagc tggcaaccat cttcgctgct tcattaacct gctgacgctt
     1561 atcaagtgta tcgagaaatg tttctctaat atctaccccg ttttttgcat tatctttatt
     1621 attattatta tttttattat ttttattttt attgattggt tccattttct ctgaaggaat
     1681 cgccacagaa ggtacgttta gaaatctatt gagatagttt gacattgcga catcaaagac
     1741 tgcacgcaaa agttccatag atgaaggcag ccggcgtatc gcctgttgaa cagcattggc
     1801 aaatgtaaaa gtgtgaagag cagtatccca gtcagagtac tcattagtga ttggaaattg
     1861 cgcaattcta agggcagcgg catatgctac tatcgcagct aattcttctt gcttcgcacc
     1921 atcttgtaga gattctataa gtacttctgc aatggcattg ggactatctc ccaacaatac
     1981 gtctacgatt ttcttgctag ctttccaggt ggtccgactt tgcttgcttt tttctaatac
     2041 tgccggaagc ttgtcaaagc aatcatttaa caacgctata atgtcgatag gatgtctcca
     2101 cgaactcctt tcctccatcc ttgtcgcttg tgcataaagt gtgacaaggc tcgtcagtac
     2161 ttgttcagcc aagttccatc ccacaacgtc aagcgcttca aaggccttat tagtaaaatc
     2221 tagaacatgc ccggaatcaa gatagcggtg gtcagtagcc gctgaaaata ggatatctgc
     2281 tatgata
//
LOCUS       DASQTF010000109         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_298164,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000109 DASQTF010000000
VERSION     DASQTF010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298164"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VE308_02600"
     CDS             complement(<1..440)
                     /locus_tag="VE308_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99891.1"
                     /translation="MNFAIAGAGVAGSYLGNMLQKRGHDVEIFEASKKDHHWPVCAWG
                     ASRHMLERFSKQAGLDFSNYIFHVGQQLKIELPNNNEESLELKGLVTYNKLSWEIDLL
                     KNVKVTYGVKVTREVFAFDKYDYIVDCTGLHRSLLPKSGQDFLIP"
     gene            complement(466..639)
                     /locus_tag="VE308_02605"
     CDS             complement(466..639)
                     /locus_tag="VE308_02605"
                     /inference="COORDINATES: protein motif:HMM:NF009623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HZA99892.1"
                     /translation="MKHLVRKCPSCNTYTLKHQCPKCDFATIDPHPPKYSPDDKYVRY
                     RIADRYLDKPEEL"
     gene            complement(647..910)
                     /locus_tag="VE308_02610"
     CDS             complement(647..910)
                     /locus_tag="VE308_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZA99893.1"
                     /translation="MTVTNEIQRLPEQGEIIIATVRQVTGHGTYVTLDEYNNMTGFLH
                     ISKIATGWIRNIERYVRPKQKAVLKVISVNRLRREVDTSLKRA"
     gene            complement(1371..1904)
                     /gene="cobO"
                     /locus_tag="VE308_02615"
     CDS             complement(1371..1904)
                     /gene="cobO"
                     /locus_tag="VE308_02615"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZA99894.1"
                     /translation="MLERGLVIVYTGKGKGKTTAALGIVLRAVGHGYKVGMIQFIKGE
                     WYYGELTSSKRLEPEFEMIAAGRGFVGIIDDDHPLEDHQRAAKQAIALAKEKIASGIY
                     DVMILDEVNYAAKLNLISQQDILDIIAARPEKTNLILTGNYAPESVMAAADLVTEMRE
                     IKHPYKKGIKAKKGIDF"
     gene            2274..3641
                     /locus_tag="VE308_02620"
     CDS             2274..3641
                     /locus_tag="VE308_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HZA99895.1"
                     /translation="MQISRIIVAGVTSGVGKTTVAVGIMHGLRKRGLRVQPFKVGPDF
                     IDPSYHSIVTNRKSRNLDVWMMGRRGVLECFASACEDADIAVVEGVMGLFDGMSGKSD
                     FASTAHVAKILDAPIVLVVDASKGARSIAAIILGFLHFDRRLRIAAIILNNVAGQRHA
                     NYITDALAGVIKLPVLGILYRNSEIKMEERHLGLVPALELKGGKSQLISRTARYIAEC
                     LDLDRVLNLCSITSLPNAREPPNRPVRARIAVALDESFNFYYPDNLIALKRSGADLVF
                     FSPLKEQKLPDGVHGMMLGGGFPEVLADSLEKNRSMVRSIRRAVDGGMPIYGECGGLM
                     YLTRSISGYKGEKKARKMAGIVQADTVMTGRLTLNYTDAECNGSIFGNTKLRGHEFHY
                     SSIENIAGDSRFAYSMNKGKGVTGSQDGFIINDNSLAAYMHLHFANRKLPERMVLSCV
                     RFSHR"
     gene            complement(3630..>4196)
                     /locus_tag="VE308_02625"
     CDS             complement(3630..>4196)
                     /locus_tag="VE308_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HZA99896.1"
                     /translation="NENEWVIVRRVIHATADFDFANKGKILFHRKAIESAFNALSDRC
                     TIVTDVDMVLAAISKKSIMDLGLKTVCYISDSIVAEQARRTGRTRSETAMRRAAKDMD
                     GGIIVIGNAPTALYEVINMVREGITKPALVVGIPVGFVSAAESKEELAKMNNLPFITN
                     IGRKGGSSAVASIINAIMLLYQSKKSSV"
BASE COUNT         1094 a          923 c          935 g         1244 t
ORIGIN      
        1 ggtatcaaga agtcttggcc tgacttaggc agaagcgagc gatgtaaacc cgtacaatca
       61 actatgtaat catatttgtc aaaggcgaat acttctcgag tgacctttac gccatatgtt
      121 actttaacat ttttcagcag atctatctcc caactgagct tgttatatgt cacgaggcct
      181 ttgagctcga gagattcttc gttgttgttt ggcagctcta tcttgagctg ctgtccgaca
      241 tgaaaaatgt aattgctaaa gtctagcccc gcttgtttag agaacctctc aagcatgtgc
      301 ctagatgcac cccaagcgca gactggccaa tggtgatctt tttttgaggc ctcaaagatt
      361 tcgacatcgt gacctcgctt ttggagcatg ttgccaagat aactcccggc gactccggca
      421 cccgctattg caaagttcat ctggaatgaa ttgtttctgg tttacttata attcttcagg
      481 cttatctaga tacctgtccg ctattcggta gcgaacatat ttatcatcag gcgagtactt
      541 tggcggatgc ggatctatcg tcgcaaaatc gcattttgga cactggtgtt tgagtgtata
      601 ggtattgcag gaaggacact ttcgtacaag atgcttcatc tttattctat gcccgcttca
      661 gcgaagtatc gacttcgcgc cttagcctgt tgacactgat tacttttaga accgcttttt
      721 gctttggcct gacatagcgc tcgatatttc taatccagcc agttgcaatc ttggagatat
      781 ggagaaagcc ggtcatgtta ttgtattcgt ctagcgtgac atatgtccca tgtcctgtca
      841 cctgcctgac agtggcaatt atgatctctc cttgctcagg aagcctctgg atttcgttcg
      901 tgacagtcat cttataatta cgatacacac ggagcaaagc gcgtacttta agattgtgtt
      961 ttaacttttc acaagctata cactatttct cctagaactc atcgaaacgg caagtctttt
     1021 gcgacggttt cataatgtat gatatctttt cttttgattt caaaaaatag gatgagaagc
     1081 gatgtatgtg aactaataga tttttgctag taacggcctg accttggagt ttatttgtaa
     1141 tttgaacgcg cccatatgct ctgcagtata tatgataata gcaagaaaat tctatttcca
     1201 tgataaaata accatttgaa tcagcagcca ataggctgcc tcgcatctct tccaaagcat
     1261 cagggaattt gactgtaatg catgtaagat tcagtgtgtg cttttaatca ctcatgattt
     1321 ttggtgttct catttgccta ctgtatggta aaaaaccatg atataaagtg ttaaaagtca
     1381 atccctttct ttgcctttat tcccttttta taagggtgtt tgatctctct catctctgtg
     1441 actaaatctg ctgcagccat gacagattcc ggagcatagt tgccggttaa gatgaggtta
     1501 gtcttttccg gccttgcagc gatgatatct aagatgtcct gctgcgatat caggttcagt
     1561 tttgcggcat aatttacttc atctagtatc atgacatcgt atatgccaga tgcgattttt
     1621 tcctttgcaa gggcaattgc ctgctttgcc gctctctggt ggtcttctaa cggatggtca
     1681 tcgtcaatta tgccaacgaa tcccctaccg gccgctatca tctcaaactc cggctcaagc
     1741 ctctttgagc ttgtaagctc accatagtac cactcaccct tgatgaattg gatcatgccg
     1801 accttgtatc cgtggcctac tgcacgcaat acaatcccaa gcgcggcagt agtcttaccc
     1861 ttgccctttc cagtgtagac aattacaagg cccctttcta gcatctgtca gcagttgata
     1921 aagcgtgtga agataaaaat caatctagta tttttcatta gacctgcctc gctgtgtctt
     1981 atgtataaaa ctctaagatg aagaaatttt tttctgctgt ctaaatccct caattgtttt
     2041 ggtcttgtgg gtattgcaga tttgctaaac acggttagca gcaaggactt atcgtaagct
     2101 ctggtccttt tccctatttg atatcttttg ctgagtccat tctgtaagat ctatttgcat
     2161 gtgaacaaca tcgtcttaat cacgttattt gccaaaatga caaggcctaa tagggcaatt
     2221 ccacgtatct caatactaaa aattaaaatt aaaatgaatt taaccaatgc tcaatgcaga
     2281 tatcccggat catcgtcgca ggagttacaa gcggagttgg caagactacg gttgcggttg
     2341 gtatcatgca cggcctcaga aaaagaggct tgcgggtcca acccttcaag gttggtcccg
     2401 actttataga cccttcatat cacagtatcg taacaaacag aaaatcgcgc aaccttgacg
     2461 tgtggatgat gggcaggcgg ggagtgctcg agtgctttgc ctcagcatgt gaggacgcag
     2521 atatcgcggt agtcgaaggt gtaatgggat tatttgacgg aatgtcagga aagagtgact
     2581 ttgctagcac agcccatgta gccaaaattc tagatgcacc aatagtgctg gtagtcgatg
     2641 caagcaaggg cgcgcgatct atagctgcca ttattcttgg ttttttgcac tttgacagga
     2701 gactccgcat agcagctatc atcctaaaca atgtggcagg acagagacat gccaactata
     2761 ttactgacgc tcttgcaggg gtaatcaagc ttccagtgct aggaatactc tatcgcaatt
     2821 ccgagatcaa aatggaggag cgacaccttg ggctagtgcc tgcattagaa ttgaaagggg
     2881 gtaaaagtca gctaatatct cggacagcaa gatacattgc agaatgtctt gatctcgata
     2941 gagtattgaa cttgtgtagc ataacctcac tacctaatgc tcgtgaacct ccaaaccggc
     3001 cagttagagc aaggattgcg gtagcccttg acgaatcgtt caacttctac taccccgaca
     3061 atcttattgc gttgaaaagg agtggagcgg atcttgtttt ttttagcccg ttaaaagagc
     3121 aaaagctccc agatggggtg catggaatga tgcttggagg agggtttccc gaagtgcttg
     3181 cagacagcct tgagaagaac agatcaatgg taagatcgat caggagggcc gtggacggag
     3241 gtatgccaat ctatggcgag tgtggagggc tgatgtattt aaccaggtcg ataagtggct
     3301 acaagggtga gaaaaaggca aggaagatgg ctggaattgt acaagcagat accgtgatga
     3361 cgggcaggct gaccctgaac tatactgatg ctgagtgcaa tggctcgata tttggcaaca
     3421 caaagcttcg aggccacgag ttccattatt cttcaataga aaacatagca ggtgattctc
     3481 gatttgcgta ctcgatgaac aaaggcaagg gggttactgg cagtcaggat ggctttataa
     3541 taaatgataa tagcctggca gcctacatgc acttgcattt tgcaaacagg aaattgccgg
     3601 agcggatggt gctctcttgt gtcagatttt cacacagatg actttttgct ctggtagagc
     3661 agcattattg catttattat tgaggctact gcagagctgc cgcctttcct tccaatgttt
     3721 gttataaaag gtaggttatt cattttcgca agttcttctt ttgattcagc cgcagacaca
     3781 aagcctaccg gtatcccaac tacaagggca ggtttagtaa tgccttctct taccatgttg
     3841 atcacttcgt acaaagccgt aggagcgttt cctattacaa taatgccgcc gtccatgtcc
     3901 ttagcagcgc gccgcatggc agtctctgac cttgtcctgc ccgtcctgcg tgcctgctca
     3961 gccacaatag agtctgaaat ataacacacc gtcttgaggc ccaggtccat gattgatttc
     4021 ttgcttattg cggcaagaac catatcaacg tctgtaacaa tcgtgcatct atcacttaga
     4081 gcattgaaag cagattcaat ggcctttcta tggaagagga ttttcccctt gtttgcaaag
     4141 tcaaagtctg cagttgcgtg tatcacacgt ctcacaatga cccattcgtt ttcgtt
//
LOCUS       DASQTF010000110         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1225051,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000110 DASQTF010000000
VERSION     DASQTF010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225051"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /gene="serS"
                     /locus_tag="VE308_02630"
     CDS             <1..1122
                     /gene="serS"
                     /locus_tag="VE308_02630"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZA99897.1"
                     /translation="LLSETVAGKKKSKQDASFELGEMKQLSAQLGQTESEAIRVEEKF
                     SQLLMTLPNLLHETVPIGIDEQDNVVVRQNGSIVRPNFNPKDHIVLATTLDLVDLGRA
                     AKVSGARFYFLKNELVKMNQALVNFALDFLSEHNYTLIQPPYMIRKEPMDGSVILNDF
                     EDVIYKIEEEDLYMIGTSEHAIASMHMDEILEGIKLPIRYAGFSSCFRKEAGAHGRDM
                     KGIFRVHQFEKVEQFVYCRPDESWREHERMLALSEEFFKQLGISYRVMLLCSGDTGKI
                     SAKTYDIEAWMAGQNAYREIVSCSNCLDYQARRLGIRFRDKTNEETRLVHTLNSTLVA
                     TERTMVAILENFQTSNGSVEIPKVLQKYMGGVEEIRARN"
     gene            1177..1872
                     /locus_tag="VE308_02635"
     CDS             1177..1872
                     /locus_tag="VE308_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HZA99898.1"
                     /translation="MAKGARKGGRVRDKWRDKQWVIVNKPAGFEPQMAINFIPVTDPA
                     QIKGRTIENTLHDMMKGNPDQSMDQHQIKVFIQIDKITDGAATTIFKGHEYAKEFIRS
                     LIRRGSSMVTFTHDYTTIDGHTFRVAMVAFTHRRVNSSKKHEIRLIGQQILAERIPQM
                     TVDQFIQEIMGNKGENIPRETSSKLGAEVLDRAKKITAIRHLGIKKTKLISTAETRAV
                     TEAKTIEPAAPAL"
     gene            1915..2370
                     /locus_tag="VE308_02640"
     CDS             1915..2370
                     /locus_tag="VE308_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HZA99899.1"
                     /translation="MSEDGSLKFSAAEVDLVLHATEDEGRVLKAIEDVLLVPSKRFSA
                     SPSEGHYKNKILLQKAILSSNEASSLAKRVISLLNSTDREHLSRLVHAYSDEKGNLYI
                     RLDKQRICQGRVSLSETDAIRIRFKPVKRYKPSAAVQSYAGLFESSLNG"
BASE COUNT          735 a          512 c          571 g          552 t
ORIGIN      
        1 ctgctttctg aaaccgttgc aggcaagaaa aagtccaaac aagatgcctc ttttgagctt
       61 ggtgagatga agcagctgag cgcacaactt ggtcagacgg aatctgaagc aattcgagtt
      121 gaggagaagt tcagtcagct gctgatgact ttgcccaacc tgttacatga gaccgtacca
      181 atcggcatag acgaacagga caatgtagtt gttcgccaaa atggcagcat cgtaaggcca
      241 aatttcaatc caaaggatca cattgtcctt gcgactacac ttgatcttgt agatctcggt
      301 cgtgcagcta aagtatcagg tgcgaggttc tacttcttaa aaaatgagct tgtaaagatg
      361 aaccaggcac tggtcaactt tgcccttgac tttttatcag agcataatta tactcttatc
      421 cagccaccct acatgattag aaaagaacca atggacggct ctgttatact gaatgacttt
      481 gaggacgtca tttacaagat agaggaagag gacctttaca tgatagggac atcagagcat
      541 gcaatagcaa gcatgcacat ggacgagatc cttgaaggaa taaagcttcc gatcagatac
      601 gcgggtttta gttcatgttt tcgtaaagaa gctggtgcac acggcaggga catgaagggc
      661 atattccgtg ttcatcagtt cgaaaaggtg gagcaatttg tctactgccg cccagacgag
      721 tcgtggaggg agcatgaaag aatgcttgca ctatctgagg aatttttcaa gcagcttggc
      781 atttcttacc gtgtaatgct tctatgctcc ggcgacacgg gtaagatatc agccaagact
      841 tacgatattg aggcttggat ggcgggccaa aatgcctacc gggagatagt ttcttgctca
      901 aactgcctag attaccaggc tcgaaggctt ggtatacgat tccgtgacaa gacaaatgaa
      961 gaaacacgtc ttgtccacac actgaacagc actcttgtcg caacagagcg cacgatggtc
     1021 gcgatacttg aaaactttca gacttcaaat ggcagcgtgg agattccaaa ggtgctccaa
     1081 aaatacatgg ggggcgtgga ggagattcga gctcggaatt gacgcatcat atatatatta
     1141 tttatttcag tcaaagacta ggaagaggaa cacgttatgg ctaaaggagc gagaaaggga
     1201 ggcagagttc gcgacaaatg gcgcgacaag cagtgggtaa tcgtcaataa gcccgcaggg
     1261 tttgagccgc agatggcgat taactttatc ccagttacag atccagcgca aataaaggga
     1321 cgtacaattg aaaatacgct gcacgacatg atgaaaggca acccggatca aagcatggat
     1381 cagcaccaaa tcaaagtctt tattcagata gacaagataa ccgatggtgc ggcaaccact
     1441 atttttaagg gacacgaata cgccaaggag tttatcagga gtcttatccg gagaggtagc
     1501 tcaatggtca catttactca tgactatacc acaatagatg gccatacttt cagagttgca
     1561 atggttgcat tcacccaccg aagagttaat tcgtcgaaaa agcacgaaat taggttgata
     1621 ggacagcaga tactcgctga gagaatccct cagatgacag ttgaccaatt catacaagaa
     1681 attatgggga acaagggtga aaatattcca agggaaacga gtagcaagct tggagctgaa
     1741 gtgcttgacc gagccaaaaa aataactgca atccgccacc tagggattaa gaagacaaag
     1801 cttatatcaa cagcagaaac acgagcggtt acagaagcca aaacaattga acctgctgca
     1861 ccggctctgt gatatagcat agctttttag ccagtttttt atactctctt tcaaatgtct
     1921 gaggatggca gcctcaaatt ttctgcagct gaggtcgacc ttgtgctcca tgccacagaa
     1981 gatgaaggca gggtactcaa ggcaatagaa gatgtgctcc tagtgccatc caagcgcttt
     2041 tcagcctcac cctcagaggg acattacaag aacaaaatac tcttgcaaaa ggcaatactc
     2101 tcgagcaacg aggcaagctc gctggctaag cgggttatct ctctcttgaa cagtactgac
     2161 agggaacatc tgtcacggtt agtccatgca tattctgacg aaaagggcaa cctctacatt
     2221 cgccttgaca agcagagaat atgccaagga agggtctcac tctcagagac tgacgccata
     2281 aggataaggt tcaagccagt gaaacgctac aaaccttcgg ccgccgttca aagttatgca
     2341 ggtctctttg agtcaagttt gaacggataa
//
LOCUS       DASQTF010000111         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_29067,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000111 DASQTF010000000
VERSION     DASQTF010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29067"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1062)
                     /locus_tag="VE308_02645"
     CDS             complement(40..1062)
                     /locus_tag="VE308_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZA99900.1"
                     /translation="MFVKDKKTVTIDELSPPKASAGDILLRMKACGLCGSDLEKVYGD
                     YGMSSGRLGHEPSGEITAIGDLVSGYSIGDRVFVHHHVPCYSCHFCRHGDFTMCKMFN
                     SSNIEPCGLSEEILVPEWNISRGGVIKLPVNMTFEKAALIEPLACCIRALSKCNISKG
                     DDIAILGAGPAGIMLALLAQSVGCGKIFLIDVNQFRLDLARDHCQVECLNISKQPNVE
                     EIIKSTTGNRGVDISVVATSNPHAMVKSFGLTRRAGTVILFGVPSKGTQVMLDASVVY
                     SNELSLVPSYAASEVETNQACSLLDNNRIDVDWIITHRFNLRQAREAILCAHEAKDSM
                     KVIVTA"
     gene            complement(1177..1320)
                     /locus_tag="VE308_02650"
     CDS             complement(1177..1320)
                     /locus_tag="VE308_02650"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZA99901.1"
                     /translation="MPVDPVCGIELKQDLALSHEHKGKVFFFCCDGCKKIFMKKPKKY
                     NRK"
BASE COUNT          609 a          468 c          418 g          606 t
ORIGIN      
        1 aacttcccga aatagtttta atacttataa gttatactat tacgcagtga ctataacttt
       61 cattgaatct ttagcctcgt gtgcacacag aattgcttct ctggcctgcc tcaggttaaa
      121 tctgtgcgtt atgatccagt ctacgtctat tctattgtta tcgagcaagc tacatgcttg
      181 attggtttca acctctgaag cagcatagct tggaacaagt gacaattcgt ttgagtaaac
      241 cactgaagca tccagcatta cctgagttcc cttactggga acaccaaaaa ggataactgt
      301 tccggctctt ctagttaaac caaaagactt gaccatcgcg tgaggattgc tggtagcgac
      361 aacagagata tctacacctc tgttacctgt ggtactctta ataatttctt cgacattggg
      421 ctgcttgctg atattgaggc attcaacctg gcaatggtcc ctggcgaggt ccaatctgaa
      481 ttggttaaca tcgataagaa atatttttcc acagcctaca gactgtgcca gaagagcaag
      541 catgattcct gcgggtccag ccccaagtat tgctatatca tctcccttcg aaatattgca
      601 tttgctgagg gctctaatac agcaggccag aggctctatg agggctgcct tctcgaatgt
      661 catgttgact ggaagtttta tgacccctcc cctagaaata ttccattcag ggactaaaat
      721 ttcttcggat aagccacaag gctctatgtt gctggaattg aacattttgc acatggtaaa
      781 atctccatgt cggcagaaat gacaagaata gcagggaaca tgatggtgta caaatactct
      841 gtcgcctatt gaatagcctg agactaaatc ccctatagcg gttatctcac cgcttggctc
      901 gtggcctagt ctgcccgaag acatgccata atcaccgtac actttttcaa ggtcagaacc
      961 gcagagcccg catgccttca ttcttagtaa tatgtcgccg gcactggctt ttggaggtga
     1021 tagttcgtct atggtaacgg ttttcttgtc ctttacaaac actgctttca acaggtaagc
     1081 attggtaatg cctcatctat aaaagtgtgg cactcgattc ctctttaagg cttcaagctg
     1141 gataaaggct ctaggggcaa atggaaaaag acaagctcac tttctattat acttcttggg
     1201 tttcttcata aagatttttt tgcatccatc acaacaaaag aaaaaaactt ttcctttatg
     1261 ttcgtgcgac aatgccaggt cctgctttag ttctatacca caaacgggat ctacgggcaa
     1321 cgcgcagcat tataaaaaag atctatatat attatcacaa atcacacgag agggtaccac
     1381 tcgtaacata gtcaggggca cagatatgtc aattattaat gccctgaaat ccagcaaacg
     1441 agcgcatgga tgctgtcggc tttaatgtca atgcttttta acactgttgt ttttgaagat
     1501 cgctttgatt cctatacata agtaatgatg tcatatccat ctacggaact aatttgtggc
     1561 aaatgcggca cgaccatatc gaaagtcatc aacctgcgac ccataagaga cgtattgcga
     1621 acacatgatg gaaagtgcaa aatgtgcggg tacaatctta atcccgctga ctttacaata
     1681 gagatcgaaa aactataaga agtagttctc tcggtttaaa ctttcagccc tatgctgcta
     1741 tgctttgtgc taataaggac catagaaata atcccgcatg cttcaaagtt tggatttgaa
     1801 acaatttatc cgtttatcct cctgagagat atattggcta aagctgtaac ctatttttag
     1861 ccaaagacca tgcgcaaatg aacgtcggcc taaattctat tctggacatc gtgtcaaaca
     1921 aaaaaagatt gtcttctaat caaacctctg aaacataggt acaatcatgc taatcgccaa
     1981 ggcgatactc gtgatacgtg aatatattac cgtcgaggcc atagcaggaa cgagagcaga
     2041 atgtgcttct tcatagttct ttctgagctc cctgaccgcc ctaaatgcat atggagcaga
     2101 t
//
LOCUS       DASQTF010000112         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_112277,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000112 DASQTF010000000
VERSION     DASQTF010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112277"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(419..598)
                     /locus_tag="VE308_02655"
     CDS             complement(419..598)
                     /locus_tag="VE308_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99902.1"
                     /translation="MNFYNVTMQIFNKEVIKIKGIADGESYQCIECEAKPAEIFQING
                     EYCLECWQEITHTNP"
     gene            complement(995..2005)
                     /locus_tag="VE308_02660"
     CDS             complement(995..2005)
                     /locus_tag="VE308_02660"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZA99903.1"
                     /translation="MRVVIVGFGVVGQSFAKLLLSRSADLYSQYGIKPRIVACVDNGG
                     SAIAPAGLDLKRLLEAKKTKGTVGGYEKGSAKFDPLQVIENVDAEVMLECTPTNLNDG
                     EPATSHITAAMRSRKNVICVNKGPLALAFPSLIELAKYNGVMLRFSGTVGGGTPILEF
                     AKRCLKGDRIISFHGILNGTTNYILSKMEEGRTYETAMKDAKEKGYAEANPSLDVDGF
                     DAAAKLVIMANWVMEMKVTMKDIKRTGIVNVRLSDVKNAAERGNAVKLLAVCDSRRLH
                     VTPTEVAKTDPICVNGTLNAVTFSSEHSGQQTIIGRGAGGMETASAMLRDLIEIRDAT
                     FS"
BASE COUNT          592 a          532 c          433 g          711 t
ORIGIN      
        1 ctttggaaga atgtcttgcg gccatgcaga agcgagccta ttgaaaagct ttgcggtttc
       61 agtcttcgat aatacgaata tcttcagtgg gatcataata acgttgcaca aacgataatt
      121 aatctgttac attaatactc agtgcttctc cctcgacctc gggctttgct taccaacgtt
      181 cttagtcttc atcatacatg ttgctcagaa atacgcacgc aagagagctc atatctgatg
      241 tgtaaatcag aggtcttgct cgatatctct tagcttcaaa gattcttgtt tggaatgatc
      301 ttgcgtgctc aaggaaaata accagcaagc gcatattctg gaataccatt taatccagtc
      361 atctcgaaag agctgagcaa tacctgttgg agaatcagtt agcgctgctt gtaaatccct
      421 agggattagt atgtgttatt tcttgccagc attctaggca gtactcgcca ttgatttgga
      481 atatttccgc aggtttggcc tcacactcga tacactggta tgactcgcca tctgcaattc
      541 cttttatttt tatgacttct ttgttgaata tttgcatcgt aacgttatag aaattcattg
      601 ctaacttgca atatttatat gtttttttga tatactatgg ccaaagatgc tctgctgtat
      661 tgctgcaaac ggagctatgg tcagaataca tatcaatcca ataggacgaa cgttttataa
      721 tttcattaca ttatacatcc aattttgaca agcgcattga caatgtatat atcttttttc
      781 tttaactcgt cctcacctgg tcttctaggc aggtaatttg ttagcaaaac tactactttt
      841 aatcttatca aatgcgcgca taccgtccac tcctgtccta tctcctacaa aacgtcgctc
      901 ctttaactgc tttacatttt gtcctctgct tttccaccta ttgctaatga ctcaataggg
      961 ttatgctatt tttcgacaca gcagttaaaa ctgttcagct aaatgttgcg tcccttattt
     1021 ctatcagatc acgtaacatc gcacttgcag tttccatccc tcctgcacct cttccaatta
     1081 tcgtctgctg tccagagtgc tccgaggaaa acgtaacagc attgagcgtg ccgttaacac
     1141 atattggatc tgtcttggca acttctgttg gagtcacgtg aagccgtctg ctgtcgcata
     1201 ctgctagcaa tttaactgca ttgccccgtt cggctgcatt tttcacatct gacaatctga
     1261 cattgactat gccagtacgc ttgatatcct tcatggttac cttcatttcc ataacccagt
     1321 tagccatgat aaccagtttt gcagctgcgt caaatccgtc gacatcaagc gaggggttgg
     1381 cctcagcata acctttttct ttggcatcct tcattgcggt ttcatacgtc cgtccttctt
     1441 ccatcttgct cagtatgtaa tttgttgtgc cattcagtat tccgtgaaat gaaattattc
     1501 tgtctccttt tagacagcgc ttagcaaatt ccaaaatggg cgttccgcca cccaccgtcc
     1561 cactgaaccg taacataact ccattatatt tggcaagctc gataagtgac ggaaaagcaa
     1621 gggcaagtgg tcccttgtta acgcagatta cattcttacg acttctcatt gccgccgtga
     1681 tgtgagaggt tgccggctca ccatcgttga gatttgtggg agtgcattca agcattacct
     1741 ctgcatcgac gttctcaatg acttgaagcg gatcaaattt ggcgctacct ttctcgtaac
     1801 cacctacggt tcctttggtt ttctttgcct caagaagcct cttgagatca aggcccgctg
     1861 gcgctatagc tgagccgccg ttgtcgacac aggctactat acggggtttg atgccgtact
     1921 ggctgtatag atctgctgac cgagagagca gtagcttcgc aaaactctgc ccaactaccc
     1981 cgaatcctac aattactaca cgcaatccag atactgctgt ttaacacggt gtggggttaa
     2041 taaaactata aaaaataagc tacgattctt atcttcgctg ttagtgcaag caacgtaaaa
     2101 aggggacaag gtacatcaag agactagtat gaatccgaaa ataaaaaagt ctattatact
     2161 caataaatca aaatagcatc aaaccgttgc gatataggtt aatcatggct catcttgtct
     2221 gatagctttg tatccaaaaa gaataatcgt ttgcggggac cagggtcc
//
LOCUS       DASQTF010000113         4925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_299029,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000113 DASQTF010000000
VERSION     DASQTF010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299029"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VE308_02665"
     CDS             <1..204
                     /locus_tag="VE308_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZA99904.1"
                     /translation="VGFEVTDLDLTIKKLKEKRVKFFKRPKEEPFGKHAIVKDPDGHL
                     ISIAEINEKSSGGFDLLGLIGAE"
     gene            complement(224..1204)
                     /locus_tag="VE308_02670"
     CDS             complement(224..1204)
                     /locus_tag="VE308_02670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZA99905.1"
                     /translation="MKNHRSTDFARLWGYSVGFYGVWIIHIGRQMKLFDHLAAMPMSI
                     EELMVSTKLYPAAVRAWCSAAQAYRLIIAKNGKLQLKKQMRRILIDKTSPDYLGGQFS
                     YLALRSLEYGAFEELFESGKTRKTMSILNAVEQATDWDHYAFLAATRSHTKLHQLLSK
                     GCRLLDVGCGTGGMLAKIHKAYPKSRLVGIDPSAKAVAIARQIAGSKPITLIKGSGES
                     MKFANEFEIVFLGESLYAIKDKERALSNCWRALKNDGTIVIVEGLLPESKFDDAANQL
                     IMGMQLDFALQGQMFMNKKDIILLLKSRFSKAHFEELGGDVYLVTATKKR"
     gene            complement(1229..2701)
                     /locus_tag="VE308_02675"
     CDS             complement(1229..2701)
                     /locus_tag="VE308_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZA99906.1"
                     /translation="MNIGHELLRIAKGEILKDRWSRQIYSVDASHYEIEPVAVVCPSD
                     VHDVQRLCEYSSSKKIPITARGAGTGLLGQSLSSGIVLDFSKHMNKIIEIDNDYVTVQ
                     PGIVKGVLDKELKKRNKFLPPDPASSNYCTIGGMIADNSSGIHSLSYGSTISFLEEIN
                     LVYADGQVGFASPVKFDDKIKKLGQLLSPHFHEIRSSYPNVSKNSCGYRLDAAIANDF
                     NPQKIFAASEGTLGMMTSAKLHILDMPTYRSLIVLGFEDLLSAVSTVPSILKFSPVAM
                     EMLDHTVVRSNESNRSDSGCLLFIEFAGNRSRNKVEECMRACKQELVDRCTVIEYASD
                     EQSISKIWMARKNALNSVMKLTLGSRKPIGLIEDTVVRPDILVEHAANLLQIYRENKV
                     DYVIYGHVGDGNMHTRPLIDTASTKEVELIHSIANKVFAQVIKRGGTITGEHGDGLAR
                     TGYIQMMYGKRITSLFSRVKKLFDPSFTMNPCKKIPCNDL"
     gene            3094..3255
                     /locus_tag="VE308_02680"
     CDS             3094..3255
                     /locus_tag="VE308_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99907.1"
                     /translation="MVVASKINSDTVAAACAFCDARIRFGESVCTECMKKYNIKSYDL
                     GNDGCGCDR"
     gene            3715..>4925
                     /locus_tag="VE308_02685"
     CDS             3715..>4925
                     /locus_tag="VE308_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZA99908.1"
                     /translation="MKMFLTMTQVHMRSTLALLIATFLFGNIPVGNAQIPPNQPLTPE
                     AQDRTFQSMSDGFSVTIPAGWVIQDVYNTDTDILLDEIMQGSRLLARLCPQEQALADI
                     EGRYSCEESSENIYIQRYPNLADESEFDSIPNGTIANEDLVNYQILKLQNLGYTDISV
                     VQNTKLTINVSSSDTNNTIAIIPANIIEMRYNNANSVDMRGYFMLAATNATSNVRMIS
                     GYNLSYEADAATLPSGRPPQPIQQIFQSFELVKEAREGELGAQDNDYDNAVAGTTQRP
                     ALIPENILSSSVGIPDRSNANASSSRSTSGIDNDVNNGSDHDYKSHLVRIVQGASTTT
                     DKAYSPNPIEVKIGDTVKWINQDTSPHTATPGQVGSAESSITADVLLEGETYSFTFDE
                     PGKYPYFCILHP"
BASE COUNT         1412 a         1092 c         1003 g         1418 t
ORIGIN      
        1 gtgggttttg aagtgacaga ccttgacctg accataaaaa agctgaagga aaagagggtc
       61 aagttcttta aaaggccaaa agaggaacct tttggcaagc atgcaatagt taaggacccc
      121 gacggccatc tcatatcgat agcagagatc aacgaaaaat cgtcaggagg ctttgacctc
      181 ctaggcctta taggtgcaga ataatggacg attgctatta gttttatcgc ttcttcgttg
      241 cggttacaag atatacatca ccgccaagtt cttcaaaatg tgcctttgag aacctactct
      301 taagcagtag gatgatgtct tttttattca tgaacatttg accttgcagc gcaaagtcaa
      361 gctgcatccc cattatcagt tggtttgctg catcatcaaa ctttgattct ggcagcagtc
      421 cctccactat gactattgta ccatcatttt tcagagcgcg ccagcaattt gataaagccc
      481 tttccttgtc ctttatagcg tagagtgatt cacccaaaaa cacaatctca aattcatttg
      541 caaatttcat tgattcgccg gagcctttta ttaaggtaat aggcttgcta ccagctattt
      601 gacgtgcgat tgcaactgct ttggctgaag ggtctattcc gaccaatctt gacttgggat
      661 atgctttatg gatcttggca agcatgccgc ctgtcccgca accgacatcg agcagtctac
      721 atcccttgga cagcagttgg tgtaattttg tatgacttcg agtggcagca agaaacgcat
      781 agtggtccca gtctgtcgcc tgctctactg cattcaagat ggacatcgtt tttctagtct
      841 tcccagattc gaagagctct tcaaacgctc catattctag gctcctcagt gcaagatatg
      901 agaattgtcc accgagatag tctggactcg tcttatcaat aaggatacgc ctcatttgct
      961 tcttcaattg taacttgccg ttctttgcga tgataagtcg atatgcttgt gcagcagaac
     1021 accaagccct gactgcagcc ggatacaatt tagtgctgac catcagttct tctatggaca
     1081 taggcatagc agcaagatga tcgaatagct tcatttgcct ccctatgtgt attatccaca
     1141 cgccgtaaaa gccgacagag taaccccaga gccttgcgaa gtcagttgag cgatggttct
     1201 tcaagtaaga ttgctcaacc tatgcggttc ataaatcatt acacggaatt tttttgcatg
     1261 gattcattgt aaatgaagga tcgaatagtt tcttaaccct tgagaagagt gacgtgatcc
     1321 gcttgccata catcatttgg atatagcccg ttcgagcaag cccatcgccg tgctcgccgg
     1381 tgatggtacc tcctctcttt attacttgag caaatacctt atttgcgata ctgtgtatca
     1441 attcaacttc ttttgttgag gcagtatcta tcagaggcct cgtatgcatg ttgccgtcgc
     1501 ctacgtgccc atagattaca taatctactt tgttttctct gtatatctgc agaagattgg
     1561 ctgcgtgctc tacaagaata tcggggcgaa ccaccgtgtc ctctattagg cctataggtt
     1621 ttcgactgcc cagggtaagc ttcatgacgc tgttaagtgc attttttcta gccatccata
     1681 tcttgcttat actctgctca tctgaagcgt attcaatcac ggtgcatcta tctacaagct
     1741 cttgcttgca ggctctcatg cattcttcta ctttgttcct gctgcgattg cctgcaaatt
     1801 caataaatag caggcagcct gagtcagatc tgttgctctc attagatctc acaacggtat
     1861 gatctaacat ttccattgcg actggtgaaa acttcaagat cgaagggact gtcgaaacag
     1921 cacttaacaa atcttcaaag cccaacacta tcaaggacct atatgtaggc atgtctagga
     1981 tgtgaagctt agcagaagtc atcatgccca gagtgccttc agaagctgca aagatcttct
     2041 gtggattgaa gtcatttgca atggcagcgt cgagcctgta tccacaagaa ttcttactca
     2101 cgtttggata agaacttcta atctcatgga aatgcgggga tagtaactgg ccaagttttt
     2161 tgatcttgtc atcgaatttg actgggcttg caaaacctac ctggccatct gcgtacacta
     2221 aattaatttc ttcaagaaag gaaatagtgc tgccatagct caaactgtga ataccactag
     2281 agttgtctgc tatcataccg cctattgtgc agtagttgct gctagccgga tcaggcggta
     2341 ggaatttgtt cctctttttt agctctttgt ccagtacccc tttcacaatg cctggttgga
     2401 ctgtgacgta gtcgttgtca atctctatta tcttattcat gtgctttgaa aaatcaagaa
     2461 ctatgccaga agatagggac tgtccaagaa gtcctgtacc tgcgcctctt gcggttatcg
     2521 gtattttctt ggatgaacta tattcacaca atcgttgaac gtcatgcaca tctgaagggc
     2581 acactactgc aacaggctcg atctcataat ggcttgcatc tacagagtat atctgcctgc
     2641 tccatctgtc tttaagaatc tcgcctttcg caatgcgaag taattcatgg cctatattca
     2701 cgatataatg tggctgccca gctataacaa attttcatgg aagcattatt gttatgatta
     2761 ttatgttttt ttcatctaca agaactcata atgaaatttg aaaatacatt gaacatagta
     2821 aaggtggttg gccgccgaat aacagtaaac atcctccaac ttatcgagca aggtttccgc
     2881 agaagttgta atcttcaatt ttttcttaat ggatactaga atcgagagcg ttgacatcta
     2941 catgatttct gctctagctc agaatctggg tttaaaggat agattccaca ttttgttaat
     3001 tagatgttcc aataaatcag agaacaaacg tgtcaaaaag cgatacgaat atccaacaag
     3061 atttaatacc tcgttctttc ctgtcctgtg ctgttggtag tagcatcaaa gataaatagc
     3121 gatacagtag cagcggcatg tgccttttgt gatgcaagaa ttcgatttgg cgagagcgtt
     3181 tgtaccgagt gtatgaaaaa atacaacatc aagtcctatg atcttgggaa cgacggctgc
     3241 ggttgtgata gataagagca agagaagggg tcagaaagtt ggtattccta atcatctaaa
     3301 tcatacagcc accacatctt catcccctgc gctaaacaat gctaattcta tgccttatat
     3361 cttacgtttt tgacaaaaat gctttttgcc ttcttttggc ggattgcact tcttaacgta
     3421 aagtttcatt tccactcttt aggcattgtt tactccgaca gctattgttg ttctaaaaat
     3481 acattgtaaa aaataagcac caaaattaat aaccgaacaa gaaaaaaata cctgttggca
     3541 atacagacgg agagaaatgt atccagacag atccacatgc atagatggga tttcgttctc
     3601 taagcgtgcc tagatgcatg aaatttttct ttgttcatgt aaagcctatt aatggaataa
     3661 tcaaccgata tcgacccaca tccttttctt gcttttcagc aggggctggc aatcatgaag
     3721 atgtttttga ccatgacaca agttcacatg cggtctactt tagctttatt aatcgctacg
     3781 tttctttttg gcaatatccc ggtaggtaac gctcaaatcc cacccaacca acccctaact
     3841 ccggaagcgc aagataggac gtttcagagc atgagtgatg gctttagtgt aacaataccg
     3901 gctggctggg taatacaaga cgtctataat actgatactg atatattgtt ggatgaaata
     3961 atgcaaggct caagactact agcccgactc tgccctcaag aacaggcact tgctgatatt
     4021 gagggacgat acagctgcga agaatcttcc gagaacatat acatccaacg atatcccaat
     4081 ctagccgacg aatctgaatt tgattctatt cctaacggca cgatcgcaaa tgaggatctt
     4141 gtcaattacc aaatactgaa gcttcagaat cttggttaca ctgacattag tgttgttcag
     4201 aacacaaaat tgacgatcaa cgttagcagt agcgatacaa ataatacaat agcaataata
     4261 ccagccaata ttatagaaat gagatataac aatgccaatt ccgtcgacat gagaggctac
     4321 ttcatgttag ctgctacaaa tgccacatca aacgtgagaa tgatatcagg atataacctg
     4381 tcttatgagg ccgacgcagc aacgctgcca tctggccgtc caccacagcc aattcagcag
     4441 atattccaaa gctttgagct tgtcaaagaa gcaagagaag gagaattagg agcgcaagat
     4501 aatgattatg acaacgctgt tgctggtaca acgcagaggc ctgccctgat tcctgagaat
     4561 atattgtcat cttctgttgg tattccggat cggagcaatg ctaatgctag tagctcaaga
     4621 agcaccagtg gtattgacaa cgacgtcaac aatggaagcg accacgacta caaaagtcat
     4681 ctagtaagaa tagttcaagg ggcatcaacc acgactgata aagcgtatag tccaaatcca
     4741 attgaagtca agataggaga cacagtaaaa tggataaacc aggacacttc acctcatact
     4801 gcgactcctg gtcaagtggg atcggcagag agtagcataa ccgctgatgt gctcttagag
     4861 ggagaaacat acagcttcac atttgatgag cccggcaagt acccatactt ctgcatactc
     4921 catcc
//
LOCUS       DASQTF010000114         5447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1044781,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000114 DASQTF010000000
VERSION     DASQTF010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044781"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..291
                     /locus_tag="VE308_02690"
     CDS             79..291
                     /locus_tag="VE308_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99909.1"
                     /translation="MIGYLAKKTKMHAVKSYLLQAVTCIAFAIAYIFAVPNGGAQGLA
                     IVLGMFGALLLFMARKQKIEQPPQNV"
     gene            complement(288..467)
                     /locus_tag="VE308_02695"
     CDS             complement(288..467)
                     /locus_tag="VE308_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99910.1"
                     /translation="MSLDDSDELAYLWTKVKFIQRYMSKHNCSYEVAEHEFHIWIEGL
                     MDSRIERANKMLNSH"
     gene            569..1309
                     /locus_tag="VE308_02700"
     CDS             569..1309
                     /locus_tag="VE308_02700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZA99911.1"
                     /translation="MIKDKVAIVTGASSGIGYATALALSRAGAKVAAGARRIDRLESL
                     QSEIIKNGGEVFIQKLDVTIKSECDVFADAVMKKWGTIDILVNNAGLQPLSFFKNLKV
                     EEWDKMINVNIRGVLYCTAAVITPMLNKKSGHIVNISSIAGRIVYPAGSVYCATKHAV
                     TAFSEGLRQEFSQMSNIRVTCIEPGVVTTELINTITDKALEKYVERTKQMEALQAEDI
                     ANAIIFAVQAPNHVNVNEILIRPITQER"
     gene            complement(1532..2059)
                     /locus_tag="VE308_02705"
     CDS             complement(1532..2059)
                     /locus_tag="VE308_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99912.1"
                     /translation="MKARKIFLGVFYFCAFVMASVTFYAIYVSLGEYLQTGRVVIGEV
                     LVETEFPFKGLAKLTTYLMIASVVGWYCVVRLGGEKVKGFPRWLKSIVQLMVLVFAVV
                     ALYEFVYNFIVWNSLITADAIRGEVMRFDDLNVEYPNPETPWNLVFATKMTLAAFLIS
                     AHCFYTISKPEKKSE"
     gene            complement(2122..3855)
                     /gene="polX"
                     /locus_tag="VE308_02710"
     CDS             complement(2122..3855)
                     /gene="polX"
                     /locus_tag="VE308_02710"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HZA99913.1"
                     /translation="MIKNSDVAKVMRDLGFLTEITEEDPNVQFRARAYYRAADTIASL
                     PENVTDIYDKYGVNGLLELPSVGKAIASKIEEYLKRGKMHHLEELKAKVPIDIDELYG
                     IEGIGPKTIRMFYDKLHIKNLADLEKAAAEGKLKTLPGFTEKKELDIFKQIEFFKRGK
                     GRLIIGEVYPLVKQIEERLQHIAGVKNAAAAGSIRRMKETIGDIDYLVAASDPERVID
                     FFVKMPEVQEVLGRGQAKAFVKLASGIDADLLVVPEESWGSALQYFTGSKEHSVQLRK
                     IAISKGLRLNEWGIFKGDKRIAGTTEEEVYKILGLQWIPPEVRENAGEIELGRQGKVP
                     KLVEYGSLKGDLQVHSENSDGTATIEEMARGAKAFGLDYIAITDHTKSLKLAGGLEEQ
                     ELLEQADRISQLNDKLREEEFCILSSAEVNIMKDGSLDIPNTVLDKLDIVGAAIHSHF
                     NLPIETQTERLIKAAKNPSVDILFHPTGRLINKRAGYPVDIEKVIEVSKDTNTVLEID
                     AHYDRLDLKDEYVRMAVRKGVKLVIDSDAHHPIHYSFLTFGVAQARRGWAIQSDILNT
                     LPADKLLKALK"
     gene            4361..4486
                     /locus_tag="VE308_02715"
     CDS             4361..4486
                     /locus_tag="VE308_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99914.1"
                     /translation="MMEWYAATGLTTITVLIGLIGFRLAKDELLLRRKRLMIGNY"
     gene            complement(4928..5401)
                     /locus_tag="VE308_02720"
     CDS             complement(4928..5401)
                     /locus_tag="VE308_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99915.1"
                     /translation="MVLSDKSQKNKEKKRWIDLINESVHTSDDIDIGDIDAVSRDFIV
                     IKRGFVNVHYYYIPIGKVEGWDGHVLWLKVPEEYIKKNYEKDVHPDPSRYYIKDFPGY
                     TAVYPEVEIIMPKYSRPVYTTKNTTPEELRVYLCELCSTAFDTEDDLSQHVSTSH"
BASE COUNT         1493 a         1186 c         1140 g         1628 t
ORIGIN      
        1 taattgtcga tggttcatct tggcattgac attgatcagt tcattcttct tactatatat
       61 cctgcagccg cgtttttcgt gataggatat cttgcaaaaa agaccaagat gcacgctgtg
      121 aaatcatatc ttctgcaggc agtaacttgc atcgcttttg cgatcgcata tatttttgca
      181 gttccaaatg gtggtgcgca ggggttggca atagtgcttg gaatgtttgg tgcactattg
      241 ctgttcatgg caaggaagca aaagattgag cagccaccgc agaacgtcta atgcgaattt
      301 agcatcttat ttgcgcgctc gatcctgcta tccattagcc cttcaatcca aatgtggaac
      361 tcgtgctcag cgacctcata actgcaattg tgtttgctca tgtatctttg gataaacttt
      421 accttagtcc agagataggc cagctcgtcg ctgtcgtcga ggctcatcaa ttcacaagtc
      481 taaaaatgta agcaaagtac ttaaacttga cgccaattgg caaaagcata ttacaacata
      541 taaaaaactg aacaaatgat ggtagcagat gataaaagat aaagttgcga tagttacagg
      601 agcaagcagt ggcatcggct acgctactgc tctggcatta tctagggctg gtgcaaaagt
      661 tgcagcaggc gcaaggcgca tagacagact ggaatcgttg caaagtgaga tcatcaaaaa
      721 tgggggagaa gttttcatcc aaaaactgga cgtcacgata aagtcagaat gtgacgtatt
      781 tgctgatgct gtcatgaaaa agtggggtac catcgatatc cttgttaaca atgctggtct
      841 tcagccactg agctttttca agaatctcaa agttgaggag tgggacaaaa tgatcaatgt
      901 caacatcagg ggagtgcttt actgcactgc tgcagtaatt acgcctatgc taaacaaaaa
      961 gtcgggacac atagttaaca tttcttctat agctggaagg attgtttatc ctgcaggaag
     1021 tgtctactgt gctacaaagc atgccgttac agcgttcagc gaaggtctta gacaggagtt
     1081 cagccagatg tctaacatac gggttacatg cattgagcca ggagtcgtga cgacagaact
     1141 tataaacact ataaccgaca aggcattgga aaaatatgtt gaaaggacaa agcagatgga
     1201 agcgctgcag gcagaagata tcgcaaatgc catcatattt gcagtacagg ctccaaatca
     1261 cgtaaatgta aatgaaatat taataagacc tataactcaa gaacggtaaa tacactcccc
     1321 gtttgggaat ccctttttgg gattcactaa ggcgagggcc gataaatgat tgttgatgct
     1381 atagattaag tacaatatta ctgagacgat tttgcagcac gaagcagaaa tttttcaaat
     1441 cctgctgacc ctcgaacaag tttgaagtaa cgcctaagca taacttcgag aaaaatcaag
     1501 acttgaaaga gtccgataaa aagaagaatg gctattctga cttcttttca ggcttggaaa
     1561 ttgtataaaa gcagtgggct gagatcaaga atgcagcaag cgtcatcttt gtcgcaaata
     1621 ctaagttcca aggagtttcg gggtttggat attctacatt aagatcatca aatctcatta
     1681 cttctcctct tattgcatct gctgtaatca gtgagttcca tacgataaag ttgtagacaa
     1741 attcatagag agccacgact gcaaatacaa gtaccatcaa ctgcacgata gatttgagcc
     1801 atcggggaaa accttttacc ttttcgccac ccaacctgac cacgcagtac caaccaacca
     1861 cagacgctat catcagatag gtggtgagct tggccagacc tttgaacggg aactccgtct
     1921 ctactaacac ctctcctatt acgaccctgc ctgtctgcag gtactcgcca aggcttacat
     1981 agatggcata gaacgttacc gatgccataa caaaggcaca aaagtaaaag actcctagaa
     2041 atattttcct tgctttcaag tcatcctgtt caaatgtcgt gcgcacaggg gtaaagtatc
     2101 tttacatgcg tgatatatga tttactttaa tgctttgagc aacttgtctg ctggcagtgt
     2161 gttaaggata tctgattgta ttgcccagcc gcgccttgcc tgcgctacgc caaatgtaag
     2221 aaaggagtaa tggataggat ggtgtgcatc agagtcgatg accaacttga ccccctttct
     2281 tactgccatt ctgacgtatt catcttttag gtcgagtctg tcatagtgtg catcaatttc
     2341 aagaactgtg ttagtgtcct ttgaaacctc gatcactttt tcaatgtcga cagggtagcc
     2401 tgcacgcttg tttatgagcc tgccagtagg atggaacagt atgtcgacgc ttgggttttt
     2461 ggcagctttt atcagccgct ctgtttgcgt ctcgattggt aggttaaaat gcgagtgtat
     2521 tgcggcgcct acgatatcaa gcttgtcaag gacagtattt gggatgtcaa gcgagccatc
     2581 cttcataatg ttgacctcag ctgaggagag gatacagaat tcttcttctc ttagcttgtc
     2641 attgagttgc gatatcctgt ctgcctgctc caaaagttcc tgttcttcaa ggccaccggc
     2701 gagcttcagg ctctttgtgt gatcagtgat tgcaatataa tcaagaccaa atgccttggc
     2761 gccgcgggcc atctcttcta tagttgccgt cccgtcactg ttttcgctat gtacttgcag
     2821 atctcctttc aggctcccat actcgaccag cttaggcacc ttgccttgcc ttccaagttc
     2881 tatctcgccg gcgttctctc gtacctctgg cgggatccac tgtaggccta gtattttgta
     2941 tacttcctct tctgttgttc ctgcaatgcg cttgtcacct ttgaaaatac cccactcatt
     3001 cagacgaaga ccttttgata tcgctatctt ccgcaattga acagaatgct ccttgcttcc
     3061 agtgaaatac tgaagagccg acccccagct ttcttcaggc actactagca ggtcagcatc
     3121 gatcccgctc gcaagcttga caaatgcctt ggcctggccc cttccaagaa cctcttgtac
     3181 ctctggcatt ttgacaaaaa aatctatcac ccgctcaggg tcgctagctg ccactagata
     3241 gtcaatatcg ccaatagtct ctttcatgcg gcggatcgaa ccagctgcag ccgcattttt
     3301 gacgcctgca atatgttgaa gacgctcctc gatctgcttt acaagcgggt agacctcgcc
     3361 tattatgagg cggcccttgc ctctcttgaa aaattcaatc tgcttaaaga tatctagttc
     3421 ttttttctca gtaaagcctg gcaaggtctt cagtttgcct tcagcggcgg ctttttctag
     3481 atcagctagg ttctttatat gcagcttgtc gtagaacatc cttattgtct ttgggcctat
     3541 accttctatc ccatacaact cgtctatatc gataggcact tttgccttta gttcttcaag
     3601 atggtgcatc ttgcctctct tgaggtattc ttctatcttc gacgcgattg ctttaccaac
     3661 cgatgggagt tctagtagcc cgttcactcc atatttgtcg tatatgtcgg taacattttc
     3721 tgggaggctt gcgatcgtgt ctgcagccct atagtaggcg cgagctcgaa attgcacatt
     3781 aggatcctct tcagttatct ctgtgagaaa tccaaggtcg cgcataacct tggcaacatc
     3841 agaattcttt atcaactggt atgagtaaac ctgccagccg ggttcttaac gtttatctgt
     3901 aggttgatat aataaaagag atgctgttat tacggaaatg gtaatgatga ctgctttcta
     3961 gctctgagcc atttccaaac tttgaaaatg tgccagggaa tagactgaaa atatacgatt
     4021 taattttgtg tctatttctg tatacaacca tgcttctact ggcaagatat aacttcctac
     4081 gtctgataac acgcgctatg ggttatccta ttcatgtttg actactcaaa agcgttgatg
     4141 atattgttcg cacgttctaa ttagtatgat gtgtatctga tagttacaaa atacatttga
     4201 gaagagttat cattctagtt gttattaata tacctcgaat gaaaaaaatg catggatcgg
     4261 tcggctggga tgtgctggaa tccgttccta ctctgactgc aatggactct gtatggagga
     4321 ttagcacgag gtcgctggtt cctatggagg ttcgagtcct atgatggaat ggtacgctgc
     4381 gacaggactg acgacgataa ctgtgctaat agggctgata gggttccgtc tggcaaagga
     4441 tgagttgctg ctgagacgca aaagactaat gattggcaac tactagaatt tttttacagc
     4501 tagcacccag ttaaaaacac aacatttcat ctagcctatt gctcaagctc accattttat
     4561 ccaagcccta aacaagtgag attcctctaa gggtagacaa gaagcatgtt attaatatgc
     4621 agccgcgacg gcttcataaa atagtataac aatttttatt tgttccattt taggctagat
     4681 aataagatgc tagttgctta tagcttttta tccataattt gatgctgtta tgagcccagc
     4741 aactacaaag gatccccagt cttcatcaga agtaccgagc tggcttacat tagcaatgtt
     4801 cgcaatgtgt actttagagg gggctcaata tttttgcagt aaagttggtt ttgattacat
     4861 ataccctata cattactgaa atcgctatca gtatcagatt attgattccc gtgttgaaat
     4921 ctgcagttca atgactggta ctaacgtgct ggcttagatc atcttctgta tcaaatgctg
     4981 tactacatag ctcacagaga tatacacgaa gttcttctgg agtggtattc tttgttgtgt
     5041 atacaggtct tgaatatttt ggcataatta tctcaacttc cggatagaca gccgtatagc
     5101 caggaaaatc ctttatgtaa tagcgtgatg ggtcagggtg cacatctttc tcataatttt
     5161 ttttgatgta ctcttctggc acctttaacc acagaacatg accatcccag ccttcaacct
     5221 ttcctatagg aatataataa taatgaacat tcacaaatcc tctcttaatt acaataaagt
     5281 cacgacttac agcatcaata tctccaatat caatatcatc gcttgtgtgc acggattcat
     5341 tgataaggtc tatccacctc ttcttttcct tatttttttg agatttatca gataagacca
     5401 tttgatatta aataaagacg cgcaatatga tatttagtga cttaatc
//
LOCUS       DASQTF010000115         5390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_113124,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000115 DASQTF010000000
VERSION     DASQTF010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113124"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1336..2604)
                     /locus_tag="VE308_02725"
     CDS             complement(1336..2604)
                     /locus_tag="VE308_02725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZA99916.1"
                     /translation="MFKLNEHLRIQPSILAVSLVSLVVMMGSTMVTPSLTLYAKQDLG
                     ANEFLVGAVIAGFAIGRLIFDVPAGFLADRLGLSRTMILGLGILIGASTLAGLASSYW
                     VLLFARLLEGVASSIYVSAAITFVLLSSDASKRGTNIGSYQSILMLGPIFGPVVGAPI
                     AEFFGYNAPYFAFAAVTLVATIIISIFSRRRKFNIEKAEEHEHENRQASMAAYLNTAA
                     IATFGFAFLRSGIYTTGVPLFGYGSLNLSVFDMGIILTMASLANLISSFFSGRLTQVY
                     GMQKPLFAAILISGLLVAVIPLSTSMVYLLVIITLIGITSGFFGQSIAWAAEQIEEKV
                     KRIGKTNYKVALGVQSHVTRGIGFNRMIGDMGLIFGPLFVGYFITVFSKDPLVWFISF
                     GSTSGVLVVASFLILGTKVKSTIPSADESF"
     gene            complement(2787..3239)
                     /locus_tag="VE308_02730"
     CDS             complement(2787..3239)
                     /locus_tag="VE308_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZA99917.1"
                     /translation="MSVVSEIMPQKVVVTVDINDNPSALDIAKLMAKHRIGSIVVIEG
                     NNNKKPIGIVTERDIIKKVSAQNKSADQVAVRHIMSSPLVTVKSIDSIDTAAETIAEN
                     KVKRLVVVEQDGSMVGILSVSDIAKKLARILTEDYSRYRSLRNLLDTD"
     gene            complement(3550..4260)
                     /locus_tag="VE308_02735"
     CDS             complement(3550..4260)
                     /locus_tag="VE308_02735"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZA99918.1"
                     /translation="MNSDLLSPVRDFNAVQARIRQTLDKGVQIDLSTESCKRFLRRKV
                     NQKVGVVILYVDIDGSTKMSMALSPEKFATILHVFSQEMSLVTSEYGGYVLKYVGDAI
                     IALFPAEYNSGQASKNAVDCAKDMQRIIKECVNPELSTRGLPQLTVKMSIDYGDVQVV
                     LYGKSIDRSHIDIVGSSISMAAKMIPLAKTWQIVIGQSMYENLDSKQNLIEMNVDPTR
                     WAYFDEKTGRSYKLYMLK"
     gene            4450..5172
                     /locus_tag="VE308_02740"
     CDS             4450..5172
                     /locus_tag="VE308_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZA99919.1"
                     /translation="MSLTDNRRKKRGQGQTGSTITEAEHIVFDQVSSNRRAEILEYLK
                     LFGYKPDRSILQVHIVGFFNKRQANARAVEQREAAARRYGGTSFVVVGRTHDPSIMDM
                     VITPKKKREETSPGYVGYLWKSKDPHKRAPHEVIPLDFTPVHVSYPADWKKIFSTKGF
                     MIALHVPTSFAKHKNDLSRALYRAGKKVDVRKGWDFIEEIRSSGYGQQAAGDPLSILK
                     IRLAKGEINTQEYEKLRQTLTT"
BASE COUNT         1523 a         1176 c         1112 g         1579 t
ORIGIN      
        1 gtttgctttt tcctcttgct cctcctccac ctaggtcgtg ccttctttgt gcttttttct
       61 tgtgcttgct agaatcagag cttttctcct ttgctgcatt tttatcaact tgtttgttac
      121 ctttagatct tcctaccatg gttaatgtat ccagaaaaat aacatataaa tatatacgta
      181 agcaggcagc ttctcgctag gcggtcgtat cagaagatga agatgatgta gatgactcta
      241 attaattgat acagaaatat ttttcattgc ggtgtaaacc ggagtcacgt ctgttgacct
      301 agctaatgga tggttgttaa atgaagggag aatcattcga tgactgtatt gggggtttac
      361 cgaattatcg tgagtttgtg tagaagggta tctctctgca aagctttatc aatatcaaaa
      421 ggcctcattt cctgccctaa ccgaactttc ataagccgtt caatataagt ttccacacta
      481 aagaggagta ttcagtttag gctgcttttt ttaccctcag atgacagttt gcaagtgatg
      541 ataattatag cagaaaagca tctattgtcg ttaaactaac ctatgttaaa agcagtctag
      601 actctctgat gaattaacat acctacacgt acggctagtt tcttcctcgc tgttatagat
      661 caccttgtag aaaagagagg aatcttagct ttatgagcac ttcctttgtt ataccgaatg
      721 caaggctaat cgaaattttc tcaaattcac aagcaccgct catggcaagc caaacttctc
      781 acatttatct ccctccactt tctgacagac ctattaattg atattgtgag ttagtgatta
      841 catcaaatgc tactatcaat cctaagatga taaattattc tgaaggatct atatatttct
      901 taattctaga ggaagtcacc tctgtaaata taagcatgtc ttacgcaact agaatccaag
      961 atctggtggg actcacaact cctgctttgc tccccaattc cccaaagcag gagggccacc
     1021 agtcttgtga cttgatgaga aactcatttt tcgtacatcc tgtgatgcaa aagaagatat
     1081 atcctctttg atattccagc cgctgctttt tcttgtatct tcgcttattg ttaagcatat
     1141 acattcttgg ttatgagctc cccgtcatgt atctttacaa tctttttatt tgatatttgt
     1201 cattatcaat tacattatgg catcttctgg taaaccatca ggacctggac accttgccaa
     1261 tgatccatac ctatgcgatg tttgtggcag gggatttcct accaaagaag aatacgatac
     1321 ccatcggcta cagcattaga agctctcatc agcacttggt attgtggact tgaccttggt
     1381 accaagtatc agaaatgaag ccactacaag tactccacta gtagagccaa atgagatgaa
     1441 ccacactaga gggtctttgc tgaagaccgt gataaaatag cctacgaaca gcggcccaaa
     1501 tatcaagccc atgtctccta tcattctatt aaatccaatt ccgcgcgtaa catgcgattg
     1561 cacgcctagt gctaccttat agttcgtttt gcctattctc tttaccttct cctctatctg
     1621 ctctgcagcc catgcaatag actggccaaa aaagcctgaa gttatcccaa tcaaagtaat
     1681 gataacaagc aggtaaacca ttgatgtgga taatggaatg actgcaacta gcaatcctga
     1741 aattaaaatt gcagcaaata ggggcttttg catgccatag acttgtgtaa gtctacctga
     1801 gaagaaagag ctaattaggt ttgcaagcga agccattgta agaattatgc ccatatcaaa
     1861 taccgataga ttcaggctcc catatccgaa gagtggcacc cctgtagtat agatcccaga
     1921 acgcaaaaag gcaaagccga aagtggcaat agctgcggtg ttgaggtagg ctgccatgct
     1981 cgcctgtctg ttttcgtgtt catgctcttc tgccttttca atattgaact tccggcggcg
     2041 gctaaaaatc gatatgatga ttgttgctac aagagttact gctgcaaaag caaaatatgg
     2101 cgcgttatat ccgaaaaact ccgcaattgg tgctccaacc accggtccaa agatgggtcc
     2161 aagcatcaat atcgactgat agcttccaat gtttgtccca cgctttgatg catcggaaga
     2221 taggagcaca aacgtaatag cagcgctgac gtaaatggat gatgcgacac cttccagcaa
     2281 ccttgcaaag agcaacaccc aataacttga ggcaaggcct gcaagggttg atgctccaat
     2341 gagaattcca agtccaagga tcattgtcct gcttaggcca agcctatctg caaggaatcc
     2401 ggccgggaca tcaaagatca atcggcctat agcaaaacct gcaataactg cgccgacaag
     2461 aaattcattt gctcctagat cttgcttggc gtaaagcgtc aacgatggtg tgaccatagt
     2521 agagcccatc atgactacaa gggagactag cgaaactgcc aatatgcttg gctggatgcg
     2581 cagatgctcg tttagtttga acattttgtc accaatatta caatgaaatg aattgaaagt
     2641 tcaggaatca atgtgttcca tgcctaattt atctttgtat ggtgacgttc aaaacaaaaa
     2701 aggagtttat atttatcttc tgtgatgtta ctaaatttac tggatccata cgttgttgct
     2761 caaagcgtca agagggatgg tggccctcaa tccgtgtcca gcaggtttct aagcgatcga
     2821 tacctgctgt aatcttccgt cagtattctt gctagttttt ttgcaatatc gcttactgac
     2881 aatatgccaa ccatcgagcc atcttgctct actacaacta gccgcttgac cttgttctct
     2941 gcgattgtct cagcagcggt gtcgatagaa tcaatagatt tgactgtgac aagaggggat
     3001 gacatgatat gcctgactgc aacttgatca gcgctcttgt tctgggcaga aactttcttt
     3061 atgatatccc tttcagtgac tatgcctatt ggcttcttat tgttgttgcc ttcaatcact
     3121 actatcgatc caattctatg ttttgccatt aattttgcga tgtccagcgc agaagggtta
     3181 tcattgatgt cgactgtaac aactactttt tggggcatta tttcactgac tacgctcaca
     3241 ctatgggagg aacttctttc aaataaatat gctcgcgcct gcctgcccta cgccaaagaa
     3301 aactaatcag tagttttgca tcagtcgctt ggtctttagt ggtcggtgag tttatcgttg
     3361 acatctagta tggattaatt gctttagaag cttgactcac ttttcagtgg atctctctat
     3421 tcttgttgag gatgatgatg atgttgttgt tgttgctatt gtcgtaccga tcattctaat
     3481 cggaaaatat tggtttgcat cacatatagg ggactgaata tcattgtttc aatcagctcc
     3541 taacaaagtc tattttaaca tatacaactt gtagctgcgt cccgtctttt catcaaagta
     3601 ggcccacctt gtgggatcaa cattcatttc tattagattt tgcttgctgt ctaaattttc
     3661 atacatggac tggccaatca ctatctgcca agtcttggcc agtggtatca tttttgcggc
     3721 catactgatg ctcgatccta ctatgtcgat atgagatcga tctatgctct tgccatagag
     3781 cacgacttgc acatcaccgt agtcaatcga catttttaca gttagctgtg gcaggccgcg
     3841 ggtgctcagc tcgggattga cacactcttt gataatcctc tgcatgtctt ttgcacagtc
     3901 tactgcgttc ttggatgctt ggccgctgtt gtattcggca ggaaaaaggg caataattgc
     3961 atcgcctacg tattttagca cataaccacc atactctgat gttacaaggc tcatctcctg
     4021 cgaaaataca tgcaatattg ttgcgaactt ctcaggggac agcgccatgc tcattttggt
     4081 cgagccatca atgtctacat aaagtatcac gacccccacc ttttggttaa cttttcgcct
     4141 gaggaagcgc ttacaggact ctgtggaaag gtcaatctgc acgcctttat caagggtctg
     4201 ccttatccgt gcttggaccg cattaaagtc tctgacaggt gataacaagt cactattcat
     4261 atcgtattac tgacaccacc atataatctg acaatatgat aatagaatgt ataatcagtt
     4321 tagaaccttc taaaaggtaa tgagcggcca tgtcagttac ggtcaaatgc atttttgcag
     4381 tcctgctgtt gaatcgcata gctgatttat catcaagtta cttatacaat gcagatcaat
     4441 cgagcctaaa tgtctctgac cgacaataga cgaaagaaaa gaggtcaagg tcaaacggga
     4501 tcaacaatta ctgaagctga gcatatagtg ttcgatcaag tttcctccaa tcgacgagcg
     4561 gagattctag agtacctaaa gctgtttgga tacaaaccag acagaagtat cttacaggta
     4621 cacatagttg gtttttttaa caagcggcag gcaaatgcaa gggcggtgga acaaagagag
     4681 gcagctgcca gaaggtacgg tggtacgagc tttgtagttg tcggaagaac gcatgatcct
     4741 tcgataatgg atatggtgat tacgccaaag aaaaaaagag aagaaacgag ccccggttac
     4801 gttggctatc tgtggaagag caaggatcct cacaaaagag caccccatga agttattcca
     4861 cttgatttca ctccggtcca tgtctcctat cccgcagact ggaagaagat cttctctacc
     4921 aaaggattca tgatagcact tcatgtgcca acgtcttttg caaaacacaa gaacgattta
     4981 tcacgagccc tctaccgagc aggcaagaag gtagatgtta ggaagggctg ggactttatt
     5041 gaagaaatta gaagtagcgg ctacggacag caagcagccg gggatccttt gagcattttg
     5101 aaaatcaggc tagcaaaggg tgagataaac acacaagaat atgaaaagct gcgtcagaca
     5161 ctgactacgt gatccctgtc tctctcccct cccccgctct gctcagtact ccttttatta
     5221 tatactcaga atgggactac ttttgtgcga tgaattggtt gaagaacaaa aggagaatct
     5281 cccgccagga tttgctggag atgctaaaac gcatagatcg cgcttatgga atagtagagc
     5341 taaatgaaag agagccaatg atacttgaaa cgaatagaaa agagcggcta
//
LOCUS       DASQTF010000116         7898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_392115,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000116 DASQTF010000000
VERSION     DASQTF010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392115"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1225)
                     /locus_tag="VE308_02745"
     CDS             complement(146..1225)
                     /locus_tag="VE308_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZA99920.1"
                     /translation="MKAVAFFEHGPVDVLQYHEDFPDPVASKDEVIIDIKYCGINHLD
                     IWTRQGIGAVGKKIRLPHICGCDIVGTTTAIKGQRVMIYPGVSCGKCEYCTSTGNRRT
                     TTTRRSRRKDNNNGNSKHENLCRQFAIIGGFSDYNGGYAERVAVPEKNIIRLPSALKD
                     EAAATLAVSYLTAWNMLQTNGASRKKKILIYGASSGVGMATIQLAKALGATVITTVSG
                     NSKYDFAKKLGAHYIIDRTRQDIAEEVKKIVPAGVDIVIDHVGAATWATSIAALRQGG
                     RMAVCGMTSGNEATVPVRMFYTKQIVMVGSLLGTRAQLQGLINFIIRKKIQPVIDSVF
                     PLHDAREAQKKMETGVHAGKILLKL"
     gene            complement(1230..3701)
                     /locus_tag="VE308_02750"
     CDS             complement(1230..3701)
                     /locus_tag="VE308_02750"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZA99921.1"
                     /translation="MEPKIQSTSWNPEIIEKAILKKWEEEEEDIYHFAIDDESKPAFV
                     IDTPPPYPSGRPWHIGAAAHYAQIDMIARVARMNGKNVLFPIGIDRNGLPVEIYTEKK
                     YKVRMRQMDRQKFLDLCKVALDDLEAEMIQIMKRMGLSGNFKEYYRTDSDEYRALTQA
                     TFIELWKRGLVYLANRPNNYCPDCGTTIADAEIVYNEIPTRLVYMKFKVREKDEKGEE
                     KKENTNDTLIIVASTRPELLFACQAVIVNPEDDRYKHLQGSHVILPLFNREVSIVPHH
                     SAKLEFGSGAVMVCSYGDQNDVQLFRELGLKEIVALNENGFATEAAGQQYAGLRVNQA
                     RTKIIEDLKNAALVEKEESITHRTPLCERSKTPIEIIPLQDYYVKQIEFVPQLKELAF
                     KLKFYPDMHRQILLKWLDSINIDWPVSRRRFYGTEIPIWYCNVCKAPNLAPSDGKYYR
                     PWKDKPPFEKCEKCGSVGSGFTGEDRTFDTWMDSSISPLYVTKFGKDKKKKMFDYAYP
                     TAIRPQAKDIIRTWLHYTMLRCVQLTGKIPWHEAWIMGYGVDEKGEKMSKSKGNVIDP
                     FPVIQKYGADTFRFWSASEANLGYDFRCSEQRIAGAQKFLSKLWNIGRFLSSFEVRDE
                     RQTPQHLEPSDRWILAELARLVRECRRGYSEYNFFVPANAIRDFTWHLFAAHYIEMVK
                     GRAYATNNNNNYSNNKDEEEDASKRSALYTLHTCFSTILSLIAPVIPFIVEELWTKMY
                     SSESTIHRQAMFRKEELEDDAEMTKYTKAIIDFNSMVWNKKKETISKETGKPLSLKDP
                     IDIPVPPELEQFGQDLKAMHNLTAR"
     gene            3875..4603
                     /locus_tag="VE308_02755"
     CDS             3875..4603
                     /locus_tag="VE308_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HZA99922.1"
                     /translation="MKFADWFPYYQGIRADFGYSTEKDQEAAKILSEMIKKKALDPKI
                     LQRKIKGKNVIVVGAGPSLEDEKVLKYIKKNKKFVKIAADSAVQFLIEKKIKPDIVVT
                     DLDGNSKFLQKAEKNGAILVVHAHGDNTDILKKLVPKFRKLIGSTQVAPVENVHNFGG
                     FTDGDRSVFLAEEFGAKKIMLVGMDFGDSIGKYSKEKVKDPELKKQKMQAGKRLLEML
                     ARQSRSQLVDTAHKPIRGFSPLTL"
     gene            4646..4816
                     /locus_tag="VE308_02760"
     CDS             4646..4816
                     /locus_tag="VE308_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99923.1"
                     /translation="MNFGRRQVKSIVGMAFAVLGGVAWYFQLYIPAAMLWGAAMVIVF
                     SLNKRRKQGQHR"
     gene            4854..5603
                     /locus_tag="VE308_02765"
     CDS             4854..5603
                     /locus_tag="VE308_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99924.1"
                     /translation="MSSGYSEEDIQRAAEIREWLIKEISDRQEEVDRLRTTLSIIDNL
                     LKQGSFKAAANLGFAAAATPTPAGPATTSTAQVQQPTRQTAAQPPAPQRQQQQQQAET
                     AAPTLRATADNGSSSDDSRNIKPLKRAKDDFLLANAEISPDAVVIVPAPGINLNANTP
                     PFKSFFLNRILEGMKSKDAEKVSQGALRESDALNYKVEEEEDDSSGGGSGTSGIIKRI
                     VINKYREKDRLQEIFNTSAWVFTRMLEKSGR"
     gene            5657..7312
                     /gene="guaA"
                     /locus_tag="VE308_02770"
     CDS             5657..7312
                     /gene="guaA"
                     /locus_tag="VE308_02770"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZA99925.1"
                     /translation="MDKLVVLDFGSQYSHLICRRIREANVYCELLPYNTPAKVIKEID
                     PKGIIFSGGPASVYAENAPRPDKEIFKMGKPLLGICYGHQVLVDTFGGKVKRSNSREY
                     GRSVLIIEGEGEENTPDLFKGLGPGIMNCWMSHADAAEILPEGFKVLARTENSFSAAI
                     GNPDKKFYGIQFHPEVVHTEKGTQILKNFAQTISGAKPEWDIESFIESTINDIRKQVG
                     NEKVLAAVSGGIDSTTVAALMHKAIGDQLSCVFINHGLLRQDEEKDVVGLFKDHLGIN
                     VIYVDAEEQFLGKLKGVSDPEEKRKIIGEEFANVFAAVVKKKKNNDEEKEDVDSFQWL
                     AQGTLYPDVIESGVSKGPAAVIKTHHNVGGLPKWLNLKVIEPLRYLYKDEVRKAAKLL
                     GVPDELLKRHPFPGPGLAVRIIGEVTPEKIRICKHASKIVEDELKSTAAFAPTSASVA
                     SPAVAAVDDANDNPGWYDRVWQAYAAVGDDRAVGVLGDERVYGYIVIIRVVESMDAMT
                     ADWSRLPYELIEKISNRITNEVEGVTWVTYAVSSKPPATIEPQ"
BASE COUNT         2104 a         1747 c         1810 g         2237 t
ORIGIN      
        1 ttacgtcgag cgcgaaaaga tgtccatact tacattttac atgggagaca aggcactact
       61 cgtcacactt gagccaaaca tcgactcaca tacagcaata gacattgcag acgatacgct
      121 tgagctacta aacggcaagt cttctctata acttgagcaa aatcttgcca gcatgtacgc
      181 ctgtctccat cttcttttgc gcttctcttg cgtcatgaag tgggaaaaca gaatctatga
      241 caggttggat cttttttctg attatgaaat ttatcaaccc ttgtagctgt gctcgtgtgc
      301 ctaagagaga tccgaccatg actatttgtt tggtgtaaaa catccgaaca ggcacggtcg
      361 cttcgttccc tgatgtcatg ccacagactg ccattctgcc gccttgtcta agagcggcga
      421 tgcttgtagc ccaagttgcc gctcctacgt gatcaattac aatgtcaacg ccggcgggaa
      481 caattttctt tacctcctca gcaatgtctt gtcttgttct gtcgattata taatgggcac
      541 ccagcttttt cgcaaagtca tacttcgaat tgcccgacac cgttgttatc actgttgcac
      601 caagcgcctt tgcaagttgg atggttgcca ttcctacccc acttgatgct ccataaataa
      661 ggatcttttt ctttctgctg gccccatttg tttgcagcat attccaagct gtaaggtacg
      721 acactgcaag agtggctgcc gcctcatcct ttagtgcgct tggtagcctg atgatatttt
      781 tttccggcac tgctacgcgt tcggcatagc ctccattata gtcactaaag cctccaatga
      841 ttgcaaactg tctacaaagg ttctcatgct tgctgttacc gttgttattg tcttttcttc
      901 tacttcttct tgttgttgtt gttcttctat taccagtgct tgtacagtac tcgcacttgc
      961 cacaggaaac accggggtag atcattactc tctgaccctt gatagcagta gtagttccaa
     1021 caatatcgca tccacagata tgaggcagcc ggatcttttt acctactgct cctattccct
     1081 gcctggtcca gatatcaagg tgatttatgc cgcagtattt gatatcaatg attacttcat
     1141 ccttgcttgc aacaggatct ggaaaatctt catggtactg cagtacgtct accggcccat
     1201 gttcaaaaaa cgcaacagct ttcatgatgt taccttgcgg tcaggttgtg cattgccttc
     1261 aggtcttgcc caaactgctc caactctggt ggcacaggga tgtctattgg gtctttgagc
     1321 gagagtggtt ttcctgtctc ttttgagatc gtctctttct ttttgttcca taccatggaa
     1381 ttaaagtcga taatagcctt ggtgtatttt gtcatttcag catcatcttc tagttcttct
     1441 tttctgaaca ttgcttgtct gtggatagtg gattcacttg agtacatctt tgtccaaagc
     1501 tcctctacga taaatggtat tactggggcg atcagtgaca ggatcgtgga aaagcatgtg
     1561 tgtaatgtat agagtgcaga cctcttgctc gcatcctcct cctcatcttt gttgttgctg
     1621 taattgttgt tattattcgt ggcatatgcg cgccctttga ccatttcgat atagtgggcg
     1681 gcaaagagat gccatgtaaa gtctctaatt gcgtttgctg gaacaaaaaa gttgtactcg
     1741 ctgtagccac gcctgcactc tctgacaagc cgggcaagct ctgccagtat ccacctgtct
     1801 gatggctcta gatgttgtgg tgtctgtctt tcatccctta cttcaaatga ggacagaaac
     1861 ctgccaatat tccacagctt tgagaggaac ttttgcgcgc ctgcaatcct ttgctctgag
     1921 cagcgaaagt catatccaag gttagcctcg cttgcagacc agaagcgaaa tgtatcagct
     1981 ccgtactttt gtatcacagg gaaaggatcg ataacatttc ctttgctttt gctcatcttc
     2041 tccccttttt catcaacacc ataacccatt atccatgctt cgtgccaagg tatcttacct
     2101 gtcagctgca cacatctcag catagtgtag tgaagccaag tgcggataat gtcttttgcc
     2161 tgtgggcgaa tcgcagtcgg ataagcataa tcaaacatct tctttttttt gtccttacca
     2221 aacttggtca cataaagtgg gctgatgcta gaatccatcc atgtgtcaaa tgttctgtct
     2281 tcacctgtga atccagaacc tacactaccg catttttcgc atttttcaaa aggtggtttg
     2341 tccttccatg gcctgtaata tttgccatcg gatggagcca gatttggagc cttgcagaca
     2401 ttgcaatacc agatcgggat ttcagttcca tagaacctgc gtcttgacac tggccagtca
     2461 atgttgattg aatcaagcca cttgagcaag atctggcggt gcatgtccgg gtagaatttc
     2521 aacttaaaag caagctcctt taattgtggt acaaattcaa tctgttttac gtaatagtcc
     2581 tgcagtggaa tgatctctat tggagtcttg cttcgctcac aaagaggagt ccggtgcgta
     2641 atgctctctt ctttttctac caaggcggca ttcttcaagt cctctataat cttggttctt
     2701 gcttgattta cacgaaggcc ggcatactgt tgccctgccg cttctgttgc aaatccattc
     2761 tcatttagtg caactatttc cttaaggccc aactcgcgga acagctgaac gtcattttgg
     2821 tctccatagc tgcacaccat tacagcgccg gagccaaatt ccagcttggc tgaatggtgt
     2881 ggcactattg agacttctct attaaacaat ggcaaaatga catgtgatcc ctgcaggtgc
     2941 ttgtagcggt catcttctgg gttgactatg actgcttggc atgcaaaaag cagttctggc
     3001 ctcgttgagg caacaataat gagtgtgtca tttgtattct ccttcttttc ctctcctttc
     3061 tcatccttct cccttacctt gaatttcatg tagacaagcc ttgtagggat ctcgttgtag
     3121 acaatctctg catcagctat cgtagtccca cagtctgggc aataattatt gggtctgttg
     3181 gcaaggtata caagtcctcg cttccacaat tcgatgaatg ttgcctgcgt tagcgcacga
     3241 tattcatcag agtctgttcg gtagtattct ttgaaatttc ctgatagtcc catccttttc
     3301 attatctgaa tcatttctgc ttccaagtca tcaagggcca ctttgcaaag gtcaagaaac
     3361 ttttgtctgt ccatttggcg catgcgcacc ttgtactttt tttcagtata aatctcaaca
     3421 ggcaggccat ttctgtcaat gccaattgga aacagcacat ttttgccatt cattcttgca
     3481 accctggcta tcatgtctat ttgagcatag tgtgcagcag cgccgatatg ccaagggcgc
     3541 cccgaaggat atggcggtgg agtatcaatg acaaacgctg gtttgctttc atcatctatt
     3601 gcaaaatggt aaatgtcctc ctcctcctct tcccatttct tgaggattgc cttttctatg
     3661 atttcagggt tccaagatgt tgactggatt tttggctcca ttgtgtgctc tgctcttctt
     3721 ttgtcctctg tcttgcggta ttattaatta ttattatttt tattatcttt aataggaatt
     3781 ctagggtgca tctctttttt gctagtattt gtaaggctca gttttcagaa gataatatta
     3841 atagtaactc ctacacatgc tctcttgtat aataatgaaa ttcgcggact ggttccccta
     3901 ctatcaggga atccgtgcag attttggata cagtactgaa aaggatcagg aagctgcaaa
     3961 aattctgtct gaaatgataa agaagaaggc acttgaccca aagattctcc agcgcaagat
     4021 caagggcaag aatgtcattg tggtaggcgc tggcccaagc ctagaggatg aaaaagtgct
     4081 aaaatacatt aagaaaaata agaaatttgt caagatagcg gcagatagcg ccgtgcagtt
     4141 tttgattgaa aagaagatca agcccgatat tgtagttact gaccttgatg gcaactccaa
     4201 atttcttcaa aaggcagaaa aaaatggtgc tatactggta gttcatgcgc atggtgacaa
     4261 tactgacata ctcaaaaagc ttgttccaaa attcagaaag ctgatcgggt ccacgcaggt
     4321 tgcccctgtt gaaaatgtcc ataattttgg aggatttaca gatggtgaca gaagcgtgtt
     4381 tttggcagaa gagtttggcg ctaagaagat aatgcttgta ggcatggact ttggcgatag
     4441 cataggcaag tattcaaagg aaaaggtcaa ggaccctgag ctcaagaagc aaaagatgca
     4501 ggctggcaag cgtctacttg aaatgctggc aagacaatct cgctctcagc ttgttgatac
     4561 agcacataaa cccatcaggg gattttcacc gcttaccctc taataatact aataacaaca
     4621 acaacaatca tcatcaatca ccatcatgaa ttttgggcgc aggcaggtaa aaagcatcgt
     4681 cggcatggca tttgctgtgc taggaggagt ggcgtggtac ttccagttat acattcctgc
     4741 ggctatgcta tggggtgcag caatggtgat tgtgtttagt ctgaacaagc gtagaaaaca
     4801 ggggcagcat cgataaaact attatatctt ttatgcagta ggatggctta ccaatgtcgt
     4861 caggttattc tgaggaagac atccaacgcg ctgcagagat acgcgagtgg cttatcaagg
     4921 aaatatcaga taggcaggaa gaagtcgacc gattacgcac tacactttcg attatagaca
     4981 acctgctcaa acagggtagc tttaaagctg ctgcaaacct tggctttgct gcagcagcca
     5041 cccctactcc agctggaccc gccacaacat caacagcgca ggtccagcag ccgactcgcc
     5101 aaacagcagc gcaaccgcct gcaccacaga ggcaacaaca acagcagcag gccgagaccg
     5161 ccgcacctac tttgagggca acggctgata atggtagtag tagtgatgat agccgtaata
     5221 tcaaaccgct caagcgcgcc aaggacgact ttttgcttgc aaatgcggag atatccccag
     5281 acgcagttgt cattgttcca gccccaggca tcaatctcaa cgccaacacc cctccattca
     5341 agtcattctt tttgaacaga attttagagg gtatgaagag caaagacgcc gagaaggtaa
     5401 gtcaaggagc gctcagagaa tcagatgcac tcaattacaa ggtcgaagaa gaagaagacg
     5461 acagcagtgg tggtggcagt ggcaccagtg gcataatcaa gcgaattgtg ataaataagt
     5521 accgcgagaa ggatcgcctg caagagatat tcaacacttc tgcatgggtg tttacacgaa
     5581 tgctagaaaa gagcggcaga taacaagaga aagatatgtg tagagagata tctagagata
     5641 tagagatgta gagaagatgg acaaattagt ggtcctagac tttggttctc aatatagcca
     5701 tctgatttgc aggcgcattc gcgaggcaaa cgtctactgc gaattattac cgtacaacac
     5761 accggccaaa gtgatcaaag agattgatcc aaaagggata atattttctg gcggtccagc
     5821 aagcgtttat gccgagaatg ctccaaggcc agataaagaa attttcaaga tgggcaagcc
     5881 tctacttggc atttgttatg gccatcaggt gctagtcgat acctttggcg gcaaggtcaa
     5941 gcgcagtaac agcagagagt acggtaggtc tgtgcttata attgaggggg aaggagaaga
     6001 aaacacacca gacctattca agggtctagg tccaggtatc atgaactgct ggatgagcca
     6061 tgccgatgca gcagaaatat tacctgaggg ctttaaggtg ctggcccgca ctgagaattc
     6121 gttctcagct gctattggca accccgacaa aaaattctat gggattcaat tccatccaga
     6181 ggtcgtccac actgaaaagg gcactcaaat cttgaagaac tttgcgcaga cgataagtgg
     6241 agccaagcct gaatgggata ttgagagctt tattgagtcg acaatcaatg acattcgtaa
     6301 gcaggtgggt aatgaaaagg tgcttgctgc tgtgagtgga ggaattgact caactacggt
     6361 tgcagcattg atgcacaaag ccattggcga ccaactgagc tgcgttttca tcaaccacgg
     6421 tctcctgcgc caggacgagg agaaggatgt ggtcgggcta ttcaaggatc atcttggcat
     6481 taatgtcatt tatgtcgacg ccgaggagca gttccttggc aaattaaaag gtgtaagtga
     6541 tccagaggaa aaacgcaaga taataggcga agagtttgcc aacgtttttg ctgctgttgt
     6601 gaaaaagaag aagaataatg acgaggagaa ggaggatgtt gattcatttc aatggcttgc
     6661 tcagggcaca ttatatcccg atgtaattga aagtggcgtg tcaaaggggc ctgcagcggt
     6721 gattaaaact catcacaacg taggcggcct acccaagtgg ctcaatctca aggtaatcga
     6781 gcctctgcgc tacttgtata aagatgaagt aagaaaggcg gcaaagctgc ttggagtgcc
     6841 tgatgaacta ctaaagaggc atcccttccc gggaccggga ttggctgtcc ggataattgg
     6901 cgaagtgact cctgagaaga tcaggatatg caagcatgca agcaagatag tagaagatga
     6961 gttaaaatcg accgctgctt ttgctcccac ttctgcttct gttgcttctc cagcggtggc
     7021 ggctgtggat gatgctaatg ataatcctgg ctggtatgac agggtatggc aggcatacgc
     7081 tgcagttggg gacgacagag ctgttggagt actgggagat gagcgtgttt acggctatat
     7141 tgtgatcata agggttgtag agtctatgga tgcaatgact gcggactggt cacgcttgcc
     7201 ctatgaattg attgagaaaa taagcaatag gataacaaat gaagttgaag gggtaacctg
     7261 ggtcacatat gcagtatcga gcaagccacc ggctacaata gagccgcagt aaaaatagga
     7321 tttttgttta cccttcaatg cggggaaact tcacgccttc ctaaaagcta tatggtttgc
     7381 ataatctatt acaactcgct taacaaaaag tggtaggtta taacaacaac aagcagtcaa
     7441 gcgagcaatt atgttcatgg atctgcatct gcaaaagtta ctaatgctcc tatgttacat
     7501 gaaatgataa aagtggaaag cgatgtcgcc aagcccacta atcctacttt tgtgaagtat
     7561 ttcgcttcga actggggtta gtatttctaa acctgtctgt tacgtcattg tagtccggaa
     7621 aatcaactac gaccagctct gctgaactct gcatccgttc ttgatatttg tcaatagcca
     7681 attgtgcttc tcttcagtat agaaagtgcc tatctttact ggcgatgagc ttttaagtta
     7741 acagagcccc ggatcacatc attttttagc tttagtgcgc gcttattcag atatggcgtg
     7801 tggcgcctgt gggagtgaat tgagatatat caaaggcact cacgatggag caggcaggat
     7861 cagttataca ttctactttt gcgatcgatg tgatgtag
//
LOCUS       DASQTF010000117         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_212188,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000117 DASQTF010000000
VERSION     DASQTF010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212188"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VE308_02775"
     CDS             complement(<1..362)
                     /locus_tag="VE308_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZA99926.1"
                     /translation="MVLSRTPEWGIKPVPDRHKILGGIDYFILWSSLGVGLLVLSAGS
                     FLSEAKFLDAIFAIIVGSAVGSILLALAGKIGSDHGIPSLITMRPAFGIRGSYLPAIL
                     NILQLIGWTTFEIMIMARA"
     gene            493..930
                     /locus_tag="VE308_02780"
     CDS             493..930
                     /locus_tag="VE308_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HZA99927.1"
                     /translation="MIIAPDIKDFIQRARVARLATVGSGFKPHLVPTVFVFDGSHFFI
                     PVDEKRKKTKPENLRRIKNIQGNSNVALLLDEYSEDWTRLAFVMIQGTASIIKSRTQA
                     DIQLRQAQKELATKYTQYQKIGIGEMCIMIKPEKVVSWRSSQL"
     gene            complement(970..1908)
                     /locus_tag="VE308_02785"
     CDS             complement(970..1908)
                     /locus_tag="VE308_02785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZA99928.1"
                     /translation="MKSGILLPQTGELATRENVLYIGREAERVGLDSVWVFERLLWPV
                     NPQTPYGGVPNAPIPVEYQSVLDPLETLTYVAGNTERISLGTSIIDMFFHNPVTLARR
                     FATLDILSGGRVIAGLGIGWSKDEYDAAGIPFRQKGARADEYLQALKRIWTDDMVEFK
                     GQFYNIPASKIGPKPMQKPHPPILLGAYTPKTFPRIVNYADGWMPIAGFVPLEQQEQA
                     INTLRETARKTNKNPSDIRIVVLTYPNLIDSSSNSSSNPQRMPMTGTIDQIGSDVERI
                     KAMGAEHIIFGYAFSPIGKDAKNMIEVTKQLARFAK"
     gene            2401..>3082
                     /gene="thiD"
                     /locus_tag="VE308_02790"
     CDS             2401..>3082
                     /gene="thiD"
                     /locus_tag="VE308_02790"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZA99929.1"
                     /translation="MKVALSIAGSDSGAGAGIQADLKTFSALGVYGCTAITAITAQNT
                     RNVAEIFGISPSIVEQQIKSVMMDMRPSAIKVGMVYSTPIIDAVYRSVKKKSKIPIVL
                     DPILAAGTGAKLLLTEAYQSLVSKLIPSSTLITPNRMEAEKLADIMIKTENDAIEAAK
                     KIKKLGADNVIVKGGHFESAYVTDLLLDSKGDINKFTNPRLEIKGIHGSGCNFSSAVT
                     AYLAKGMAL"
BASE COUNT          944 a          658 c          626 g          854 t
ORIGIN      
        1 gcccttgcca ttatcataat ttcaaatgta gtccagccga tcagctgcag gatattgagt
       61 attgcaggca gataagagcc tctgatgcca aatgccggtc tcattgttat gagcgacggt
      121 atcccatgat cacttcctat cttacctgcc agggcaagga gaattgatcc taccgccgac
      181 ccgacaatga ttgcaaaaat ggcatcaaga aatttcgctt cagaaagaaa tgagcctgca
      241 gaaagaacta gcagtcctac accaaggcta gaccataaaa tgaagtagtc tatcccgcca
      301 agtatcttgt gcctgtctgg aaccggcttg attccccatt ctggtgtcct tgacagaacc
      361 aaacttcatc aatcacaaaa aagctagcat caataatagt ctattgattg aaaatccttt
      421 ctgtatcgat ggctgtcttc catgttccca agcgcacaca tatttttccg atataacaca
      481 ataagtatat ccttgattat cgcacctgat attaaagatt ttatacaaag agcaagagtc
      541 gcaaggttag caacagtagg ttctggattc aaaccacact tggtaccaac tgtctttgta
      601 tttgacggca gtcatttctt tatccctgtt gacgaaaaaa gaaaaaaaac taaaccagaa
      661 aacctcagaa ggataaaaaa catacagggt aattctaacg ttgcactact ccttgacgaa
      721 tatagtgaag attggactag gttggccttt gtaatgattc aaggaacggc gtcaatcata
      781 aaatccagaa cacaagcaga tattcagctg cggcaagccc aaaaggaact cgctacaaaa
      841 tatacacaat atcaaaaaat cggaataggt gaaatgtgta tcatgataaa gccagagaaa
      901 gtggtgtctt ggagaagctc tcagctctaa gcaatcattt gcccctagaa gaatatgtct
      961 tgtcttcttt tatttggcaa atctggcaag ctgttttgta acttctatca tattttttgc
     1021 atctttgcct attggagaaa atgcataacc aaaaattatg tgctctgctc ccattgcttt
     1081 aatccgctct acgtcacttc caatttggtc gatagtgcct gtcattggca ttctctgtgg
     1141 gttcgaagag gaatttgaag aagagtctat caaattaggg tatgtcaaca caactatacg
     1201 aatatctgat gggttcttgt ttgtctttct ggcagtttct cgcaaagtgt ttatagcctg
     1261 ttcctgttgt tccaatggta caaatcccgc aatcggcatc caaccgtccg catagtttac
     1321 aattcgaggg aaggtctttg gagtgtatgc cccaagtagt atggggggat gaggtttttg
     1381 catgggttta ggtccaatct ttgaagctgg gatgttgtag aactgacctt tgaactcaac
     1441 catgtcgtca gtccaaattc tctttagcgc ctgcaaatat tcgtctgccc tcgctccctt
     1501 ctgtctgaaa ggtatgcccg ccgcgtcata ctcatctttt gaccatccaa ttccaagacc
     1561 cgcaataact cgaccacctg ataagatatc gagtgtagcg aatcttctcg caagtgtgac
     1621 aggattgtga aaaaacatat caattataga agttcctaag gaaattcgct cggtattacc
     1681 tgccacgtat gtcagtgtct caagcggatc gagtacgctt tggtattcga ctggtatagg
     1741 agcgttaggt actccgccat aaggtgtctg ggggttgact ggccaaagaa gacgttcaaa
     1801 tacccatacg gaatcaagtc caacccgttc agcctccctt ccgatatata gaacattttc
     1861 tcttgttgct aattctcctg tttgtggcaa aagtattcca gacttcataa gaaatatgag
     1921 gacaataggc ctgcgaagta ttaaaggagt ttaacaaacg gttctgagaa ttttgggggt
     1981 tttttgttag tagtctgttt tcaagtgaac aaactacaga ttttgcatta taattatctc
     2041 agactttata gtgattagat gtatgatcta actacaaaat gcttggatag ttcttcatag
     2101 atatcatcaa accaattaaa aaattatctt tcttatcaga atttgcggga tatagcatat
     2161 caatcgattt atgttccttc gaatccgatg atgctttggc catcatttaa ccctaggtag
     2221 tataattgaa agcctgtgtt tattgcaagt caagtaacgg ctgacatgat ccaatagcat
     2281 aaacctgagg tatttacaca gacaatatcg cttgaataaa caatgctggt caatcgctgc
     2341 aaaaaacctg caggatcccc caagaaagat tgaaaagcca acctccatta tcattctgag
     2401 ttgaaggtag cattatcaat agctgggagc gactctggag cgggtgctgg aatacaagcg
     2461 gacttaaaga cattttctgc acttggcgtc tatgggtgca cagcaataac tgccataact
     2521 gctcagaaca caagaaatgt cgcagagata ttcggtatat cgccatcaat agttgaacaa
     2581 cagataaaat cagtcatgat ggatatgcga ccgagcgcca tcaaggtagg catggtctac
     2641 agcacgccta taatagacgc ggtttatcgc tcagtcaaga aaaaatcaaa gattccaata
     2701 gtgcttgatc cgattcttgc agcagggact ggggccaaat tactccttac tgaagcatac
     2761 caatcactcg tatcaaaact gatcccatcc tccactctaa taactccaaa caggatggaa
     2821 gctgagaagc tagcagacat tatgatcaaa actgagaacg atgcaattga agctgccaaa
     2881 aagatcaaaa aattaggagc agacaatgtc attgtcaagg ggggccactt tgaatctgca
     2941 tatgtgaccg acctgctact cgacagtaaa ggcgacatca acaaatttac aaaccctcgg
     3001 ctagaaataa aggggattca tggttctggc tgcaactttt cctcagcagt cacagcatat
     3061 cttgcaaaag gcatggcgct ta
//
LOCUS       DASQTF010000118         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_764542,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000118 DASQTF010000000
VERSION     DASQTF010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764542"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..467)
                     /locus_tag="VE308_02795"
     CDS             complement(72..467)
                     /locus_tag="VE308_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99930.1"
                     /translation="MPKPGFKTITVKEHVFNKYYELYKKAKKEHKVGEGISFSSFVTN
                     MMQETMAKYEAFVKHAPFLEKIAIEQERVIIKDNKRDRIAEVMVRNGELQCLLDEKTD
                     CIHIGFVYALPEVYAVLQKKKGIIKSRPQ"
     gene            680..1642
                     /locus_tag="VE308_02800"
     CDS             680..1642
                     /locus_tag="VE308_02800"
                     /inference="COORDINATES: protein motif:HMM:NF020014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99931.1"
                     /translation="MLKETKSPIDSSIFRSVKNERCASCGNVLQEQTIIVIAQQPPLK
                     PSQNNISPPSPHHAVHALPSPLPMIFETAYDVQQRSTKLAFDIAEVDSLLDFSDSGGS
                     ICVASNRKSRDGGWHANTLVTRLCVRALMSRRYGGFGSPSVIFIDAGNCSDIYQCVNF
                     ARQYGLDIQKVLDSIMVSRPFTIHQLAGLLICELESAAMHRSGAKLVVVSDMLKMFSQ
                     DASDPQVDHDEAQWLLREIAMSLQRISAQALVVISVNNNNNCPSQYQRLLPHCDNWID
                     ITAATASSDTSHNLRLEITNHRRDDSKRHCLSLPEREVQFVLAR"
     gene            1962..2255
                     /locus_tag="VE308_02805"
     CDS             1962..2255
                     /locus_tag="VE308_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99932.1"
                     /translation="MSLFPTDDILAKLQDQWKGYADALRQEDKEAFLTMIEKCYAHAA
                     AINAKGEPFPNEALLMSLIFSQYKMINWLVPKVAELSAMLDELEKEGEDKERT"
     gene            2252..>4377
                     /locus_tag="VE308_02810"
     CDS             2252..>4377
                     /locus_tag="VE308_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HZA99933.1"
                     /translation="MTTTTTSTTGWLFDAYPQGDRMIFWIKQEKDGKIVKLEDNSWSH
                     SIYVASDNKHNLASVAKDERVSSFIKRYEFVKKYEKIIDKTKSEVLKLTLTNSSQAQK
                     LAKCIMLGGRFGQFHLYNVDILPAQSYFYEHDIFPLAFCQVTVSGDVDDNSGSRLTWS
                     CKDYDDVWSIDYRLPKFKTIHLSIRPLKQESKLPKFTDKIDSISIRNENEIIEIDGTD
                     DAQILFSLTEIMSALDPDFVFTEGGDSFGFPYLAHRAKVNDCSNILVLGREPFRATTR
                     KPIKDGITYFSYGRMHYRPSSTQLFGRVHIDKHNSFILDEAGMEGLYEVARICRMPLH
                     NASRATIGRCMSSLQCYHASVNDILIPWKSAFAEHFKTYSQLFVADRGGMIFEPRTGV
                     HEQVGELDFSSLYPSIMCAKNLSGETVLCACCPESKLRVPELGWHICEKKIGIVPQSL
                     EILLKKRAQYKQLLKSPHTDPKTKQVYDSRQGSLKWILVTSFGYLGYSNSKFGNIDAH
                     IATCAFDRQVLLKAVRVAESHGFRVLHGIVDSIWVVKQGTAREDYLKLKEAIKQNTGF
                     AISFEGIYKWVAFVHSKRSNKHIPVPNRYFGVFEDDSSLKVRGLEMRRRDNPPFFDKF
                     QAEVLKVIAKGNTIEEVMMLMPQVKDVLNKYVQLLKDQKVPLQELVFTKQLSKDSDSY
                     VVNTIESTSLRQLAAEGRMLRAGEVL"
BASE COUNT         1272 a          938 c          947 g         1220 t
ORIGIN      
        1 gttggaacag tcagcgtgat tagctgactt cttttctacc catgtcataa gaatagaggt
       61 cttgcttgct gctattgggg tcttgactta ataattccct tcttcttctg aagcactgca
      121 tatacttcgg gtagcgcata gacaaaccca atatgtatgc agtcagtctt ctcatctaga
      181 agacactgca gctccccgtt ccttaccatt acttctgcta tcctatcgcg cttgttatct
      241 ttaattatta ccctctcttg ttcaattgca atcttttcaa ggaatggcgc atgcttgaca
      301 aaggcttcat actttgccat cgtttcctgc atcatgttag ttacaaagct cgaaaagctt
      361 atgccttcgc ctactttatg ctctttcttt gctttcttgt ataactcata gtatttgttg
      421 aaaacatgct cctttactgt gatggtttta aagcctggct tcggcatatt gttaatgaca
      481 gggacggtta gaggacagaa ctttataaga tttttgctat cccctaagtc atgagatgtt
      541 gaccttagct gtcagaaaaa tgaacaaact accttcatta ggaacattac tgtaatgtta
      601 ccgtcctcat atgaggtttg gtcaaaacca tcagcagcag cagctgcttt accaatacag
      661 ctgctcacgt tgtcgtacag tgctaaaaga gacaaagagt cctatcgaca gcagcatttt
      721 ccggtccgtt aaaaatgaaa gatgtgctag ttgtggtaac gtcttacagg agcaaacaat
      781 aatagtaata gcgcaacagc cgccacttaa gccttcacag aataacattt ctcctccttc
      841 tcctcatcat gctgttcatg cattgccgtc gccgttacca atgatatttg agaccgccta
      901 tgacgttcaa caacgatcca ccaagctcgc cttcgacata gccgaagtag actcattgct
      961 cgacttttca gacagtggag gaagcatttg tgttgccagt aatagaaaaa gcagggatgg
     1021 cggctggcac gcaaataccc ttgtcacaag gctatgcgtc agggccttga tgtcaaggcg
     1081 gtatggtggc tttggctcgc cttctgtaat attcattgat gcaggcaatt gctcagacat
     1141 ttaccagtgc gtgaactttg ctcgccaata tgggcttgat atccaaaaag tcttagatag
     1201 tataatggta agcagaccgt ttaccattca ccagctggca ggactactca tatgcgagct
     1261 tgagtcagca gctatgcacc gctctggtgc taaacttgta gtagtatctg acatgctgaa
     1321 aatgttctcc caagatgcat cagacccaca agtagaccat gatgaagcgc aatggttgct
     1381 cagagaaata gcaatgtcac tgcagagaat atcggctcaa gcattagttg ttatttcagt
     1441 aaataataat aataactgtc catcgcaata ccagagactc ttgccgcatt gtgataattg
     1501 gattgatatt actgctgcta ctgcatcatc tgacacatcg cacaacttgc ggttagaaat
     1561 aaccaaccat cgccgtgatg acagcaagcg tcactgctta tcactgcctg aaagagaagt
     1621 gcagttcgtt ctcgctcggt gaatatatac atgtacaatg tgtgtgtcat cagtaccata
     1681 ccatcattta ttcttgcgcg tgcaatgaaa gtaagagatg gaagtcattt cctaacatat
     1741 tgcaagacag atcagagaca gaaagaagtt tgagagatgt tctttgatat tcccaagttt
     1801 tacttgctat tattgtactc cgcctgtccg actccatcct ctacttattt caattctgct
     1861 gtgtactgtt tcagacagct ggttaagtgt gtgcccaagc aagtagagca gatgctggct
     1921 gaaattagga attggaaaga agaaggagat ctatcataat aatgagcctg tttccaactg
     1981 acgacattct tgccaaactg caagatcagt ggaaaggcta tgccgatgct ctgcggcaag
     2041 aggataaaga agcgttcctt acaatgatag agaaatgtta cgcgcacgcc gctgcaatta
     2101 atgcaaaagg tgagcctttc ccaaatgaag cattgcttat gtctctaata ttctcgcaat
     2161 ataagatgat taattggttg gtgccaaagg tagctgaact aagtgccatg ttggatgagc
     2221 ttgaaaaaga aggagaagat aaggagcgca cttgactact actactactt ccactaccgg
     2281 ctggctcttt gacgcctatc ctcaaggcga cagaatgata ttttggataa agcaggaaaa
     2341 ggatggcaag attgtcaagc ttgaagacaa cagctggtca cactcaatct atgttgcttc
     2401 tgataacaaa cacaacttag cttcagtagc aaaagacgag cgcgtttctt cattcataaa
     2461 gcgctatgaa tttgtcaaga aatatgagaa gattattgac aaaaccaagt ctgaagtatt
     2521 gaaattgact ttgacaaact cgtcgcaagc ccagaagctc gccaagtgta tcatgcttgg
     2581 cggcaggttt ggacagttcc acctttataa cgttgatatc ttgccggcac aatcatattt
     2641 ttacgaacat gatatctttc cgcttgcttt ttgtcaagtt accgtgtccg gtgacgtcga
     2701 tgataatagt ggtagtaggc taacatggtc gtgcaaagac tatgatgacg tgtggtccat
     2761 cgactatagg ctaccaaagt ttaagactat ccacctgagc atcagacccc tgaagcagga
     2821 gagcaagcta cccaagttta cagacaagat agactcaata tctatcagga atgaaaatga
     2881 gataattgaa attgatggta ctgatgatgc tcagatactc tttagcctta cagaaatcat
     2941 gtctgctctc gatccagact ttgtctttac tgaaggtggc gattcatttg gctttccata
     3001 tctggctcac agagccaagg tcaacgattg cagcaatatc cttgtccttg gtcgggagcc
     3061 attcagagca actactagaa agccaatcaa agatggaata acctatttct cttacggcag
     3121 gatgcattac cgtccttcat caacccagct ctttggcagg gtgcatattg acaagcacaa
     3181 ctcatttatc cttgacgagg caggcatgga ggggctttat gaagttgcca gaatatgcag
     3241 aatgccactt cataatgctt cacgcgctac aattggacgc tgtatgagca gcctccagtg
     3301 ctaccatgca tcagtaaatg acatcctaat tccttggaaa tccgcatttg cagaacactt
     3361 caagacctat tcacagctat ttgtagcaga tcgcggcggt atgatatttg agccgcggac
     3421 aggcgtgcat gagcaggttg gtgagcttga cttttcttcg ctttatccaa gtatcatgtg
     3481 tgcaaagaac ttgtcaggcg agacagttct atgcgcctgt tgccctgaat caaagctgcg
     3541 tgtgccagag cttggctggc atatctgcga aaagaagatc ggtatcgtac cacaatcgct
     3601 tgaaatattg cttaagaaac gggcgcaata taagcagcta ctaaagtctc ctcatacaga
     3661 tccaaaaaca aaacaagttt atgactcaag acaaggatcg ctcaaatgga ttttagttac
     3721 aagcttcggt taccttggat atagcaattc aaagtttggc aatattgatg cacatattgc
     3781 tacatgtgct tttgacagac aagtgctcct aaaagcagtc agagttgcag aatctcatgg
     3841 ctttagagta ctgcatggta ttgttgattc tatttgggtg gtcaagcaag gtactgcacg
     3901 agaggattat ctgaaactaa aggaagcgat aaaacagaat acaggttttg caatatcatt
     3961 tgaagggatt tacaaatggg ttgcatttgt ccactccaag aggagcaaca agcatatccc
     4021 ggtgccgaat cgatactttg gtgtatttga agacgacagc agcctgaaag taagaggctt
     4081 ggagatgcga aggcgcgaca atccgccatt ttttgacaag ttccaagcag aagttctaaa
     4141 agtcattgcc aagggcaata caatcgaaga agtcatgatg ttgatgcctc aggtcaaaga
     4201 tgttcttaac aagtatgtgc aattattgaa agaccaaaag gttccgcttc aggaacttgt
     4261 ctttacaaag cagctatcca aagattctga cagctatgtc gttaatacta ttgaaagcac
     4321 ttcactacgt caactagcag cggagggcag aatgttgcga gcgggcgaag tcttaca
//
LOCUS       DASQTF010000119         5827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_32548,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000119 DASQTF010000000
VERSION     DASQTF010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32548"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VE308_02815"
     CDS             <1..742
                     /locus_tag="VE308_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HZA99934.1"
                     /translation="AAKEVGSKLVHIEAGLRCFDLSVPEERVRIQIDSTSDYLFPPTE
                     LARTFLSYEEIEKNVTVTGNLVVDVCRRLAKVADEYGRNYDLPDKYLLLTMHRQENVD
                     EPDRLEMLRKHLSGINHKIVFPIHPRTKNNLAHYGITLPPNVIAIEPAGYLEFLYLLK
                     NCRLVMTDSGGVQEEAIVLRRPCVTLRHVSERWETLLLKANVLFPIHRRDPLSNVIEM
                     MLDAKIERNPYGENVARTMIQLIKQIIQ"
     gene            851..3004
                     /locus_tag="VE308_02820"
     CDS             851..3004
                     /locus_tag="VE308_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZA99935.1"
                     /translation="MNRYSGAFSLAATMILVLQTTFLAYGQISSAQLFGCQHYGGVMH
                     CDLLLNEMEGNEVRGNSSLINPLTTTDLLFVNGKFGQAAEMRGEYRESIEIMNSPELN
                     PNQFSISFWMKSTTIEPYGHIISHSNRAQTSGWQFDVFRSGGGGQSDSQVAATLRFGV
                     FNTNGTFFSPVDIQVPTDKFTLITGTFDGSRVRLYMDGLLVGETEFLGEYNADPGLPL
                     RIASPAYCSSCNRWSGIIDDVRMYDRTLGEDEIKQLFDMSDASLGLIGYWKFDGDTRD
                     VLQRHDGTTVTMLTSMVFAADGRLFFTEKNTGAIRIMTPEHKILDKPFASVDDLYASW
                     EQGMLGLAIDPEFDINHHIYVYYTAVIDIENGNGGEVINRVLRFTDSNNTGIDPTVIM
                     DNIPASRGYHSGGAILFGPDGKLYITVGDATEHIFAQDPSIVIGKVLRINKDGTIPQD
                     NPYPNSPVYTMGHRNMYGIAFDDHGIGLVTENGDVRYDEINLITNGGNYGFPTSQPAN
                     LPPERANDSSIKPLRSYWETIAPTQMIYYEGDVVPELKGMFLFGSFTGDIYALKLSED
                     RKRIIQEIKIELEHYPFVPTVGIAQSPDGRIYYGGYQIYTLDSIGEREQMLFTISLNS
                     SSALDIGEIRVDEEQKRIMIDASMKSRLTPDATLIARIPRALLDNVTTVAVDGQQGPR
                     AIEFNINEFGPDYTIVSVNIGSQAASEQDLKLVIY"
     gene            complement(3127..4491)
                     /locus_tag="VE308_02825"
     CDS             complement(3127..4491)
                     /locus_tag="VE308_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZA99936.1"
                     /translation="MERKRIIIFFLMAVVLVTGPSIGFSSWGVDENNRLIQKASLSGD
                     MNNNLISTQLSSNNPNNTFQVLPDPSPPSISDPNIRVEKVITGLEMPTSMAFLDNDDI
                     IITQKDNGRVRLVSNGMLQPQPILQVPVVNNSERGLLGVAIANTTTNSTTKTVFLYYT
                     EPVGDQVKNRLYRYEWNGAGNLTGGQLILDLPGEPGPNHDGGKIAIGPDRMLYTVIGD
                     LNRDGMLQNVIEGPPPDDTSVILRIDYNGNGVGNAISGGGADLSKYYAYGVRNSFGFD
                     FDPLTGILWDVEDGPAEYDEINIVLPGFNSGWNRVMGPIGREAVTAQENLVQFQGSQY
                     ADPVFSWLRSEGVTDMEIFNSSSLGERYAYNIFVGDINNGNLYFFTINSNRTGLELSG
                     IAGLEDLVADNIEELNAVIFGRGFSGGITDIETGPDGYLYILTLSGDLYRMVPGAPPS
                     PATL"
     gene            complement(4998..5294)
                     /locus_tag="VE308_02830"
     CDS             complement(4998..5294)
                     /locus_tag="VE308_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HZA99937.1"
                     /translation="MQIIAIGSRVFVTSFQLAGVKGVNVETSAQALTEINKMDENSGI
                     GLVLLSDDIGKDIRYQLTSLRAKRPIPLIFELPSPGSKKEKVDYRALLKQILGV"
     gene            complement(5384..5569)
                     /locus_tag="VE308_02835"
     CDS             complement(5384..5569)
                     /locus_tag="VE308_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99938.1"
                     /translation="MFLSYIAYLKAKNDPITRPGMAMISDIPASSHRNTAKTAQLSEP
                     IISIRIAGFVAAKSHLM"
     gene            complement(5625..>5827)
                     /locus_tag="VE308_02840"
     CDS             complement(5625..>5827)
                     /locus_tag="VE308_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99939.1"
                     /translation="TRSRWAPSADFGTVYSYEEIKRRYRPSNVGGGMWDISDKASYGI
                     WKATILLFACLAALLLYIMLLS"
BASE COUNT         1599 a         1310 c         1287 g         1631 t
ORIGIN      
        1 agcagcaaag gaagttggct ccaagcttgt tcacattgaa gcgggcctcc ggtgctttga
       61 ccttagtgtt ccggaagaaa gggtaaggat acagatcgat tctacatcgg actacttatt
      121 tcctccaacc gaacttgcta ggacttttct atcctatgag gaaatagaga aaaatgtcac
      181 tgtaactggc aatctcgtcg tagatgtctg ccggcggctt gcaaaggtcg cagatgaata
      241 tggtagaaat tacgatcttc ctgacaagta tttgttgctt actatgcata gacaagaaaa
      301 tgtcgacgaa cctgacaggc ttgagatgtt gagaaagcat ctatcgggta taaaccacaa
      361 gatagtcttc cccatccatc ccaggacaaa aaacaacctt gctcattatg gcatcaccct
      421 gcctccaaac gtcattgcaa tagaaccagc cggttatttg gaatttcttt atcttttgaa
      481 aaactgccga ctagtcatga cagattcagg gggagttcag gaagaggcaa tcgtacttag
      541 gcgaccatgc gttactttga gacatgtttc agagcgttgg gagacgctcc tattgaaagc
      601 aaatgtgctt tttcctatac atcggcgtga tcctttgagc aatgttatcg aaatgatgct
      661 tgatgctaag atcgagcgca acccctatgg agaaaatgtt gcaagaacca tgatccagct
      721 gataaagcag atcatacaat agatttctct ataagtttat gtggttcgtt taaagacgta
      781 caatccggat gtctaaccga tatggttgag cgtctcaacg attaaattat tttcatactc
      841 ttgttttact atgaaccgct attctggtgc cttttcgctt gcagccacta tgatacttgt
      901 attacagacc acttttttag cctacggaca gatatcctca gctcagctct ttggatgcca
      961 gcactatggc ggtgtgatgc attgtgacct actcctaaat gaaatggagg gaaatgaagt
     1021 caggggtaat tcatccctca taaatcccct aaccactact gatttgctat ttgtcaatgg
     1081 caagtttggc caagcagcgg aaatgcgagg ggaatatagg gagtcaattg agataatgaa
     1141 ttcacctgag ctcaacccaa accaattttc tatttcgttc tggatgaagt ctacaacgat
     1201 agagccatat gggcatatca tatcccatag caacagagct caaacatctg gctggcagtt
     1261 tgacgttttt agaagcgggg gcggtggcca atctgactcc caagttgcag caactcttcg
     1321 ttttggggtt ttcaatacta atggcacgtt cttttcgcct gtcgatattc aagtcccaac
     1381 agacaagttt acactcataa ccggcacgtt tgacggttca agagtcagat tgtacatgga
     1441 cggactgctt gttggcgaaa cagagtttct aggcgaatat aatgcggatc ccggcttgcc
     1501 gttgaggatc gcatctccgg cgtactgctc atcatgcaac agatggtcag gtattattga
     1561 cgacgttcgt atgtacgatc gaacacttgg cgaggatgaa atcaagcagc tgtttgatat
     1621 gtcggatgct tctttgggct tgattggtta ctggaaattt gacggtgata caagagatgt
     1681 cttacaaaga catgatggga cgaccgttac aatgctcaca agtatggtct ttgccgcaga
     1741 cggcaggctt tttttcactg aaaagaatac aggggcaata aggataatga cccctgaaca
     1801 taagatccta gacaagccgt ttgcaagtgt agatgatctg tatgctagct gggagcaggg
     1861 aatgttgggt cttgcgattg accccgagtt tgatatcaac caccacatat acgtatacta
     1921 taccgctgtc atagacatcg aaaatggtaa cggcggcgaa gtcatcaacc gtgttctaag
     1981 attcacagac agcaacaata cggggattga cccgacagta ataatggata acattcctgc
     2041 ttcacgtgga tatcactccg gtggcgctat tctctttggt cctgacggca agttgtacat
     2101 cactgttggt gatgccactg agcatatttt cgcccaagat ccatcgatag tgataggcaa
     2161 ggtactgcgc ataaacaagg acggtacaat accgcaagat aacccctatc cgaattcccc
     2221 cgtctataca atgggccaca gaaacatgta tggtattgca tttgatgatc atgggatagg
     2281 tctggttact gaaaacggcg acgttcgcta tgatgaaata aacctcataa caaatggcgg
     2341 gaattatggt tttcccactt cccagccggc caacctgcct cctgagcgag cgaacgactc
     2401 atcaatcaag ccactgagaa gttactggga aacaatagca ccaacacaga tgatttacta
     2461 tgaaggcgat gtggtgccag agcttaaggg gatgttcctc tttggcagct ttaccggtga
     2521 tatctatgct cttaagttaa gtgaagacag aaagcggata attcaagaga taaagataga
     2581 gctagagcac tatcccttcg ttcctacagt tggaatcgcg cagtcacctg acgggaggat
     2641 atactatggc ggctaccaga tatacacact tgattcaata ggtgaaagag agcagatgct
     2701 ctttacgata agcctaaact cgtcttcagc gcttgacatt ggagaaatca gagttgatga
     2761 agagcaaaaa aggataatga tagatgcaag catgaagagc aggcttactc cagatgccac
     2821 acttatcgca agaataccaa gggcccttct agacaatgta actaccgtag cagttgacgg
     2881 tcagcaaggt ccacgtgcaa ttgaatttaa catcaatgaa tttggccctg attacactat
     2941 agtcagcgtc aatatcggct ctcaagctgc aagcgaacag gacctaaagc tggtcatata
     3001 ctgagctcaa acatcattgc ctagctttaa cacggtggat aactgccggt tagtatgctg
     3061 ttgctagtac ggtcatttcg acctatatat gtggagcaag tctgaagtat aagacaaaaa
     3121 attgccttat aaggtggctg gagacggcgg cgctcctggt accatccggt acagatcccc
     3181 cgatagtgtc agaatgtaca agtagccatc gggccctgtt tcaatatcag tgattccgcc
     3241 ggagaagccc ctgccaaata ttactgcatt tagctcctcg atgttgtcgg ccaccaagtc
     3301 ttccaaacca gcaatgccgc ttagttcgag cccagtcctg ttgctgttga tggtgaagaa
     3361 gtacaagttg ccgttgttta tatcccctac aaagatgttg taggcatatc tttcaccaag
     3421 gctcgacgag ttaaagattt ccatatcggt gacgccctcg gatcggagcc agctgaaaac
     3481 agggtccgca tattgcgatc cttgaaactg cacaagattc tcctgagcag tgaccgcttc
     3541 tctcccaata ggccccatga ccctgttcca tccgctgtta aagccgggta gaacaatatt
     3601 tatttcgtca tactcggctg gaccatcttc aacgtcccaa aggatccctg ttagtgggtc
     3661 aaagtcaaaa ccaaagctgt ttcgtacacc ataagcataa tacttactaa gatcggctcc
     3721 tccgcccgat atggcattcc cgactccatt tccattgtaa tcaattctta atatcacaga
     3781 tgtatcatcg ggcggtggac cttctattac attttggagc atcccatccc tgttaaggtc
     3841 ccctatcacg gtgtatagca tgcggtcagg tccaattgct atctttccgc catcatggtt
     3901 gggtccgggt tcgccgggca gatcgagaat aagctgtcct ccagtcaggt tacctgcccc
     3961 attccattcg tatctgtata gcctgttttt cacttgatct ccaaccggct cagtgtaata
     4021 tagaaataca gtctttgtgg tgctgttagt agttgtgttt gcaatagcca ctccaagcaa
     4081 tcctcgctcg ctattgttta caacgggtac ctgaagaatt ggttgcggct gaagcattcc
     4141 atttgataca agacgcactc tgccgttgtc cttttgtgtt ataataatgt catcattatc
     4201 caaaaacgcc atgctggtag gcatttctag gcctgtgatg accttttcaa ctcttatgtt
     4261 tggatcagat atactaggtg gagatgggtc gggcaaaacc tgaaatgtgt tattaggatt
     4321 attgctactt agttgagtag aaataagatt attattcatg tcccccgata aagatgcttt
     4381 ctgaatgagc ctattgtttt catctactcc ccaagagctg aagcctattg atggacctgt
     4441 gacaagtact actgccatta aaaaaaaaat tattatccgc tttctttcca tgcaaaagtg
     4501 atgaatgatt tctgcttatt atgtattgta gagaacgata aggtactgaa aaacttgaat
     4561 cgttcatgtg gcttttatct agaagaattt taacagctca agcttatgtc ctactcttca
     4621 tatcttgttg cgaggaagca tttccgtcaa tgtataatat ctacactcat ggtttgtggt
     4681 gggacaacaa accttattga cactgcaagg aattacattg gccatctgca cgtcacgtca
     4741 tgttagtgct agatcgtcga cgattaactg acagaatcgt gaaagacagt tgctgcaatg
     4801 acactcgcaa ctaacgaact ataaggtttt acatactggt tgcatcatac agctatactt
     4861 tgtagatgaa atcctgcgaa cctctattat tcctcttact actagccttc atggttaggc
     4921 tagtatataa cccctgacca attaccgaaa tggttaagat ggacacatat atcggaagag
     4981 cctgggagct ggtttgacta cactccaagg atttgtttta gaagcgcacg ataatctact
     5041 ttttcttttt tgcttcctgg tgagggcagc tcaaagatga gcggaatagg cctcttagcc
     5101 cttagacttg taagctggta gcgaatatct ttacctatgt cgtcgctcaa aagtacgagc
     5161 ccaattccgc tgttttcgtc catcttattg atttctgtga gagcttgggc agatgtttcc
     5221 acattgactc ctttcacacc tgccagctga aaactggtga caaaaactcg gctgcctatg
     5281 gcaattattt gcatcagaat cctccttaat tcgcctttga ttttggtccc tttttattcc
     5341 ttttgagcaa cgtctcggaa aacgactttt atatcaacac atattacatc aggtgagact
     5401 tggctgcaac aaaacctgcg attctgatgc tgatgatcgg ttcactaagc tgcgccgttt
     5461 tagctgtgtt cctgtggctg ctggcaggaa tatcgcttat cattgccatc ccgggcctgg
     5521 tgatcgggtc gttttttgcc tttaggtatg ctatgtatga taagaacaaa gctcgtaaaa
     5581 gaagaagagg aagagcaaaa gcgcaataga aaaaggcgca accgctagct cagaagcatg
     5641 atgtataaca atagtgcggc caagcatgca aacaagagta ttgttgcctt ccatattccg
     5701 taactggcct tgtcactgat atcccacatg cctcccccaa cattgcttgg cctatagcgc
     5761 cgttttattt cctcatagct gtatactgtc ccaaaatccg ctgatggcgc ccatcttgac
     5821 ctagtcc
//
LOCUS       DASQTF010000120         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_32680,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000120 DASQTF010000000
VERSION     DASQTF010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32680"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VE308_02845"
     CDS             complement(<1..339)
                     /locus_tag="VE308_02845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020640.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HZA99940.1"
                     /translation="MSETAAGIEYTKVAEIKGPLMIIDGITRASFDELVEIETPDKER
                     RLGRVLEVGFGKAVVQVFEGTTGLAITGTKAKFLGKTMQLPVSQELLGRVFDGLGRPN
                     DGLPEPVADKF"
     gene            complement(332..2119)
                     /locus_tag="VE308_02850"
     CDS             complement(332..2119)
                     /locus_tag="VE308_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HZA99941.1"
                     /translation="MKAKGKIVWVSGPAVKADGMSSAKMYETVEVGSTKLIGEVIRVT
                     GDVAFIQVYESTSGLKPGEPVVGTGQPLSVLLGPGVIGKIYDGIQRPLDEIAQKSGSF
                     IGRGVATTPVDLKKKYKFLPAVKVGDEIGAGSVLGFVEETPLLTHSILAPPNHPGGKI
                     VEAVKENNFNIDQVVAITENNGNRIELKMYHKWPVRKPRPYAERYDPSVPLITGQRII
                     DTYFPIAKGGTGAIPGGFGTGKTVTLHQIAKWADSKVVVYIGCGERGNEMTEVLVEFP
                     HLIDPRSGRPLMERTVLVANTSNMPVAAREASIYTGVTIAEYYRDMGFDVVLVADSTS
                     RWAEALREMSGRLEEMPAEEGYPSYLASRLAEFYERAGRVRAMGSPERSGSVTIVGAV
                     SPSGADFTEPVTTHTIRFIKTFWALDTRLAYSRHYPSINWMQSYSGYLEDVSKWWKQN
                     VSAEWYGLRAESYNILQREDTLKEIVRLLGPEALPDEEKLVLEVARMIKIGILQQNSF
                     DKVDTYCSPQKQLKQVRLMVQFYKNAQRAIKDGLSLVDIRAMPIITSLLKAKFEITDE
                     EVSKLDNLEQEMNDQFGKLGQREEVKVLV"
BASE COUNT          540 a          568 c          435 g          677 t
ORIGIN      
        1 aaatttatca gccaccggtt ctggtaggcc gtcattgggc ctgcccaacc cgtcaaacac
       61 acgaccaaga agctcctgtg agaccggcaa ttgcatagtt ttgccgagga attttgcctt
      121 agttcctgta attgcaaggc ccgtagttcc ttcaaatact tgaacaactg ctttcccaaa
      181 accgacctcc aaaactcttc ccaaccgtct ctccttgtca ggggtctcaa tttctaccag
      241 ctcgtcaaag gatgctcttg ttatgccatc tatgatcata agaggtcctt tgatttcggc
      301 aacctttgta tattctattc cggctgcggt ctcagacaag gacctttacc tcctcgcgtt
      361 ggccaagctt gccaaactgg tcattcatct cttgctctaa attatccagc tttgatacct
      421 cttcatctgt aatctcaaat ttggctttta gaagagaggt tattataggc atggccctaa
      481 tatcgacaag cgacaatcca tcttttatcg ccctttgtgc atttttgtaa aattgaacca
      541 ttaacctcac ttgctttagt tgtttttggg gactacaata agtgtctact ttgtcgaaag
      601 aattctgctg taatatgccg attttgatca tacgtgccac ttcgagaact aacttttcct
      661 catcaggcaa agcctcaggt ccaaggagtc ttactatttc ctttaatgtg tcctctctct
      721 gaagaatatt atatgattct gccctcaaac cataccattc cgcactaacg ttttgcttcc
      781 accattttga tacatcttct agatatccag agtacgactg catccaattt atggatggat
      841 aatgcctaga gtaagcgagt ctagtatcaa gagcccaaaa agtcttgata aatctgatag
      901 tgtgggtcgt aactggctca gtgaaatctg ctcctgatgg tgacacggct ccgacaattg
      961 tcactgaacc tgatctttct ggcgagccca ttgctctgac acgcccggca cgttcataga
     1021 attcggctag ccttgatgca agatatgagg gatagccctc ttctgctggc atttcctcaa
     1081 ggcggccaga catttcgcgt agcgcttcag cccacctgct tgtagaatct gctacaagaa
     1141 cgacatcgaa gcccatatcc cggtagtact cggctatggt aacgcctgtg taaattgaag
     1201 cctctcttgc ggctactggc atattacttg tatttgctac tagaaccgtt ctttccataa
     1261 gcggtctgcc ggatcttggg tcgatcaaat gagggaattc aacaagcacc tcggtcatct
     1321 cgttacctcg ttcgccgcaa ccgatgtaaa ctacgacctt tgagtcagcc cacttggcta
     1381 tctgatgcaa cgtcactgtt tttccagtcc caaaaccgcc aggaatagca cctgttccgc
     1441 cttttgctat gggaaaatat gtatcgatga ttctctggcc tgttataagg ggaactgatg
     1501 gatcatatct ttcagcatag ggacgtggct ttctgacagg ccatttgtga tacattttta
     1561 attctatcct gttcccatta ttctctgtaa tcgccactac ttggtcgatg ttgaagttat
     1621 tttcctttac tgcctcgacg attttacccc ctggatgatt aggaggagca agaatgctgt
     1681 gagtcaaaag tggagtctct tcgacaaaac ctaaaaccga acctgctcct atttcatctc
     1741 caacctttac ggccggcaaa aacttatact tcttttttaa atcaaccgga gtggtggcga
     1801 ctcctcttcc aataaaggag ccagactttt gcgctatttc gtcaagaggc ctctgaatac
     1861 catcgtaaat ttttcctatg actccggggc ctagcagcac agatagcggt tgaccagtgc
     1921 ccactacagg ctcgccaggt tttaatccgg atgtggattc gtaaacttgg ataaatgcaa
     1981 catcacctgt tacccgaatt acctccccga ttaatttcgt gctacctact tctacggttt
     2041 catacatctt ggctgatgac ataccgtcag cttttacagc aggaccactc acccagacaa
     2101 ttttaccttt ggctttcatc tttacttatc tcctcttaat gtttggacta ttgctttcct
     2161 tattaaaggt ttgagccttt ccgctcggga gtcaagagta ctatcgtaca tcagggagcc
//
LOCUS       DASQTF010000121         4104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_492401,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000121 DASQTF010000000
VERSION     DASQTF010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492401"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VE308_02855"
     CDS             complement(<1..1055)
                     /locus_tag="VE308_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZA99942.1"
                     /translation="MVVVGSSLMVALNGLGAGFGKFIDESFSMLAPNILFVSSAQQDQ
                     SASGPFGGGGPPPVPKITLNTAVETRIRSLPFVSDVISSYQGQVELESSGKTLSTSVF
                     SIDPAKLYAIAPTLEFEEGSAMRSNDPSAIYIADQIAYPPGENAPFATIGQTMRATYS
                     FVDENGNQQEESRSFVVRGIMKPTGNPTIDNAVVFNEDAGNALMHKSGTYDGFLVAAE
                     SGEFVKNVEEEIRKLYGNDIGVTSPEAILETIREFTGGFTVFILGIALVALLVGAVGI
                     ITTLYTSVMERTKEIGTMKAIGAQNGNVLSLFMAEALMIGILGATSGLLAGIAGGYVL
                     TAGLGSAGGPEGDDTSSG"
     gene            complement(1291..1968)
                     /locus_tag="VE308_02860"
     CDS             complement(1291..1968)
                     /locus_tag="VE308_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA99943.1"
                     /translation="MHVKKLFKIYDSAAGRVIALRDVTFKVNKGEFVAIVGPSGSGKS
                     TLLNMIGALDRPTSGKVFIGGVDIFSLSNSEIATMRNRMIGFIFQSYNLINRTTVQRN
                     VELPAIIKGMSRTNRIRRAKKLLEMLGIGDKANFKPSNLSGGQQQRLAIARALMNNPT
                     IILADEPTGNLDTKTGNEVFNFLRILSQKYRRTIVMVTHNPELAEATDRAIYIKDGTV
                     EKEVLNQ"
     gene            2287..>4104
                     /locus_tag="VE308_02865"
     CDS             2287..>4104
                     /locus_tag="VE308_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99944.1"
                     /translation="MANLSFDNSAYGQEQSVDDERSEDSSNDDTVTPTTPREDDTSED
                     DQPPPQQVQPPPQQVQPPPQQVQPPPQQVQPDSATSGTPREEDDTSEDNASDQSTGPV
                     RFTFVDSFWTDYTSLGGVIASTSSESVTAQPLPPAIKQEVEPGEGEAVLAVVLRNRGF
                     ADATSISGSLDLPSGFRAIVTPEDVDSDTALASYNGIVNAGQTFTLYFRVEITQESEV
                     GREYTGELKIRYFKVDEQEEEDTRSTTLDIPFRLSGRVILNTAAVMASSSSISTQGTE
                     SILSHIVSANPGIVNPLKIEISNNGSAIATGVIVNVLPPGTVSSSNTQTGGDGTGSSL
                     SSNNSAESTTTSGPAQQSISSSSSPATMVIVGSPTFNVGLIDANATKEIVPTIFPADT
                     AAGTLTTLNIQILYNDAYGNKKTLNQVLGVQILPESPQSGLSVLPSSSTSLEAAPESS
                     LLPLTRTSLGAPSNNSTTGQSEVGRSSNSSNTNSSSSTEAQSIQIAAGSVQDLSFDIT
                     NNNDAGASISDVVVSLTSESNEIRILGDSRWNLQRIAAGSQQELSTRVYASTSLIGSP
                     VFFTVTMQYIRNGNELRTESFQLGAIVVGNVQISVNDVTI"
BASE COUNT         1124 a          978 c          866 g         1136 t
ORIGIN      
        1 ccactacttg tatcatctcc ctcaggtcct ccagctgaac ccaatcccgc agttaacaca
       61 tagcctccgg cgatacctgc aagcaatcca gaagtggctc caagaatacc tatcatcaga
      121 gcttctgcca tgaaaaggct caggacatta ccattctgcg ctccaatggc cttcatggtg
      181 cctatctcct ttgtcctttc cattactgac gtgtacaggg tcgtaatgat tcctactgct
      241 cctacaagaa gggctacaag cgcaattcct agtatgaaaa ccgtaaaccc acctgtaaac
      301 tcccttatcg tctctagtat ggcctctgga gatgtgacgc ctatatcatt tccatatagt
      361 ttccttatct cctcttctac gtttttcaca aactcgcctg attccgcagc taccaagaat
      421 ccgtcatacg tgccggattt atgcattaga gcgtttcctg catcttcgtt gaatacaact
      481 gcgttatcga ttgttggatt acccgttggc ttcattattc ctcttactac aaagcttcta
      541 gattcttcct gttgattgcc attttcatca acaaatgaat aagtagctct catcgtttga
      601 cctatggttg caaaaggcgc attctcacct ggcggatagg ctatctggtc agctatgtaa
      661 atggcagatg gatcatttga acgcattgca gaaccttctt cgaattctaa agtaggtgct
      721 atggcataga gcttagcggg atcaatagaa aaaacggagg tggaaagagt cttgcctgat
      781 gattctagct ctacttgacc ttgataagat gagatcacat cgcttacaaa aggaagagag
      841 cgtatccttg tctcaacagc agtgtttagt gtaatcttgg gaacaggcgg tggaccgcca
      901 cctccgaagg gaccagaggc ggactgatcc tgctgagcac ttgacacaaa cagtatattt
      961 ggagcaagca tgctaaagga ttcatcaata aactttccaa aaccagcgcc aagcccatta
     1021 agcgcaacca tgagactact tcccactaca accattagta tagtaaggat cgatcttact
     1081 tttctatcct ttagagcgtc gaatgaaagt aagaagatct ccctgatatt catttagcac
     1141 gcaattcctt ttcttctttt cttttagttt tttgctcttg ttggtctaga tgttgcaggc
     1201 acataaaaac agatattcgc gcaagcggca tcgcattgag cgagcaatcc aaccctccaa
     1261 cccaaccaaa caccaatgtt ctttgattaa tcactggttc aagacctctt tttcgactgt
     1321 tccatcttta atgtagatgg ctctgtcagt ggcttctgca agctcgggat tatgcgttac
     1381 cataacaata gttctcctgt acttttgcga aagaatgcgc agaaagttaa agacttcatt
     1441 tccagtcttt gtgtccaaat tgccagtcgg ctcgtctgca aggattattg tggggttatt
     1501 cattaatgct cgggctattg ctaggcgctg ctgctggcct ccacttaaat tggagggttt
     1561 gaaattggct ttatctccta tacccagcat ttcaagaagc ttcttagcac gacgaatacg
     1621 gttggtgcga ctcatgcctt taattatcgc aggtaactca acatttcttt gaaccgtagt
     1681 tcggttgatt aagttgtatg actggaatat aaaaccaatc atcctgtttc gcatagtggc
     1741 tatttcagaa ttactcaatg agaaaatatc gacaccccca atgaacacct ttccagaggt
     1801 aggtctgtcc aaggcaccaa tcatattcag aagggttgat ttaccgctcc cagaaggacc
     1861 gacaatggct acaaactcac ctttgtttac cttgaaagtg acgtctctta gcgctatcac
     1921 tcttccagca gcagaatcat atatcttgaa tagtttcttt acgtgcattg ctacattatc
     1981 ttccctttta gtctcatgct gatagaaaga gtgaattctt ttactgtcaa cttccttgct
     2041 catcggaatg tattcaaata actctaagtg atatatagat atagtgtaat ttctgtccag
     2101 atatgaggac agtatatgct tttattgcgg tcctgctaaa attggtcggt ggttcttgca
     2161 ggagaattgg gatgtaagat gctcacaatt tccacacaaa tagtcaggaa ttgtgattat
     2221 tattctgtgg gatctctggc ttgtattacc cttttgcttt tgggatcaat tccgtattat
     2281 ttttacatgg ctaatttatc atttgataac agcgcatacg gtcaagaaca aagcgttgat
     2341 gatgaacggt ccgaagacag cagcaatgat gataccgtta ctcctactac accaagagaa
     2401 gatgacacaa gtgaggacga tcagcctcct cctcagcaag tgcagcctcc tcctcagcaa
     2461 gtgcagcctc ctcctcagca agtgcagcct cctcctcagc aagtgcagcc tgactctgct
     2521 acttctggta caccaagaga agaagatgac acaagtgagg acaatgcctc agaccaatct
     2581 actggccctg ttaggttcac gtttgtagat tcgttttgga ccgactatac ttcgcttggg
     2641 ggcgtcatcg ctagcacatc ctctgaaagc gtcactgctc agccactacc accagcgata
     2701 aagcaggaag tagagcctgg agaaggagaa gccgtcctcg cagttgttct gagaaaccga
     2761 ggctttgccg acgcaaccag cataagcggt tcacttgatt taccgtctgg ctttagagcc
     2821 attgttactc cagaagatgt agactcggat accgcgttag caagctataa tggaatagtg
     2881 aatgcaggtc agacatttac cctttacttt cgcgtggaaa taacacaaga atctgaagta
     2941 ggcagagaat acacgggtga gcttaagata cgttacttta aggtggacga acaggaagaa
     3001 gaagatacga gatcgacaac gctggatatt ccattccggc taagcggcag ggttattttg
     3061 aatactgctg ctgtcatggc atcctcctct agtatcagca cgcaaggcac ggagtccatc
     3121 ttatcccata tcgtaagtgc gaaccctggc attgtcaatc cattgaaaat agaaatcagt
     3181 aataatgggt cagctattgc tacaggagtt atagtaaatg tgctgccacc aggcactgta
     3241 tcctcttcta atactcaaac tggaggagat ggaactggca gcagtcttag cagtaacaac
     3301 agcgcagaaa gtacaacaac ctctggacct gcgcagcagt caatatcttc gtcatcctct
     3361 ccagccacaa tggtgattgt tggctcgcct acatttaacg ttggtcttat agacgcaaac
     3421 gcaacgaagg aaatagtgcc cacaattttt cctgcagata cagccgccgg gacccttaca
     3481 actttgaaca tccagatatt atataatgac gcttatggaa ataagaagac tttaaaccaa
     3541 gttctcggtg tccagatatt gccggaatca cctcaatcag gcttgagcgt tttgcccagt
     3601 agttcgacat ccttggaagc agcaccagaa tcttcactgc ttccactgac acgcaccagc
     3661 cttggcgcgc cttccaacaa tagcactacg ggtcaaagtg aagtcggcag gagcagcaac
     3721 agcagcaata cgaattcgtc gtcatctact gaggcccagt ctatccagat tgctgcaggg
     3781 agcgttcagg atctatcatt tgatatcacg aataataatg atgcaggtgc atcgatatcg
     3841 gacgttgtgg tttctctcac ctcagaatca aacgaaatca gaattctagg cgattcgcgc
     3901 tggaatctgc agagaatagc cgccggatca cagcaggagc tctctactag ggtctatgcc
     3961 tcaacttctc tgatcggaag cccagtgttt tttacagtaa ccatgcaata catccgaaat
     4021 ggcaacgagc tgaggacaga gtctttccag ctcggcgcaa tagttgttgg gaatgttcag
     4081 ataagcgtaa atgatgttac gatt
//
LOCUS       DASQTF010000122         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_495441,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000122 DASQTF010000000
VERSION     DASQTF010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495441"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            567..947
                     /locus_tag="VE308_02870"
     CDS             567..947
                     /locus_tag="VE308_02870"
                     /inference="COORDINATES: protein motif:HMM:NF002586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HZA99945.1"
                     /translation="MAEGNKVKRIAITLLFFFIASLCEIGGGYLVWLWLRDGMSWMLG
                     ALGGFVLFLYGVVPTFQPSHFHRIYGAYGGIFIVMAMIWGWIFEGIAPDRFDIIGAAI
                     ALAGVIIIFYMPRKGEEPVWSQQK"
     gene            989..1291
                     /locus_tag="VE308_02875"
     CDS             989..1291
                     /locus_tag="VE308_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HZA99946.1"
                     /translation="MFFAAAIAEIGGGYLVWLWLREKKTITLGALGGIILFVYGIIPT
                     LQPAEFGRVYAAYGGIFIVSSIIWGRIVDKKNPDRYEIIGSIVAVVGTLVIFYAPR"
     gene            complement(1425..1958)
                     /locus_tag="VE308_02880"
     CDS             complement(1425..1958)
                     /locus_tag="VE308_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZA99947.1"
                     /translation="MAKAEKKKAAKPKVSAIPKGYHSVTPVLIVKGAEQAIEFYKKAF
                     GAKELHRAYMPGGNIILHAEIQIGDSRIMLNDEFPEMNCKSPQSVGGASTALYVYVKD
                     VEKIFTQAPAAGATVTMPVMDAFWGDTTGQLIDPYGHVWSFASRKRNLSSKEMQKAQE
                     EWLAEMSKGQQQAQKPQ"
     gene            2230..>2626
                     /locus_tag="VE308_02885"
     CDS             2230..>2626
                     /locus_tag="VE308_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99948.1"
                     /translation="MSILSKNSITVTTATAAIILLFVITGVIPYGYHQQNHLRFTAEA
                     GGGGPCIDYETSENTIAINCNASFLDVVQTINDPQILEEQEEEEEEENGQYILNANLE
                     VADGVTFAMTSNEDGLQYLKIAGANGIIVH"
BASE COUNT          722 a          530 c          603 g          771 t
ORIGIN      
        1 tacagcagat gtttcagagc ctcgaattta tcagatgatg atcaaataag aggctacatt
       61 tagtagtagt aaagtatgac aataacagca taggaagtac ctggcaacct gagcaagaag
      121 atagattttc tgcaggacaa tagggagttt actaaagtgc aagtgcaaca aaggaacatc
      181 cgatgcagac tgaataagaa agtaaaagtg ctgtatggta ctgaattacc gaccgactct
      241 acaggctaag ggcgtattat ataccgccgc aacagctgaa gtcaaaggtc gcaacaacgc
      301 agcactgctg caaactagca ataaggacac aaaacgcgct taggcaattg ccctggcaat
      361 tatgatacat ctactcaggc tgcagcagca aaaaagaagc agactggagg aatccagaac
      421 gatttggttc ttttgaggga ttctgagaat agtaggaacc tagtgagctt tttcgaaaga
      481 ttgttggagg aaggtatagc acccgagata tagaaataat cataaagtag acggactact
      541 ccgcctcctg ttgcgggcag taaatcatgg cagaaggtaa taaagtcaaa agaatcgcca
      601 taacattgct tttcttcttt atagctagtc tgtgcgagat tggtggaggc tatctggtat
      661 ggctatggct tcgcgatggc atgagttgga tgctaggcgc tcttggaggt tttgtgctat
      721 ttctatatgg agtagtgcca acatttcaac cctcacactt ccacagaatc tatggagcat
      781 acgggggcat ctttattgtc atggctatga tttggggatg gatttttgaa ggaattgctc
      841 ctgacaggtt cgatattata ggcgctgcaa ttgctctagc tggtgttatc atcatattct
      901 acatgccaag aaaaggagag gaacccgtct ggtcgcagca gaagtaataa tacaagcttc
      961 tcctcttcct cctcttgtca cactggcact gttctttgca gctgcgattg cagagattgg
     1021 cggaggctat ctggtatggc tatggctacg agaaaagaaa actattacac taggcgcttt
     1081 gggaggcatc atcctctttg tctatggaat cattccaacg ttacagccag ctgagtttgg
     1141 tagagtatat gcagcatacg ggggcatatt catagtatct tcaatcattt ggggtaggat
     1201 agtggataaa aagaatcctg atagatacga gattataggc tcaatagttg ctgtcgttgg
     1261 cacccttgtc attttttatg ctcctcgctg agtctatttt atttaatcat gtgttgtccg
     1321 ggtgtgaaaa aagatgcgcc gactgtcgat gcttgccttc tgaatgtaag actacccctt
     1381 cgccaggcca atgtcgcagt tgtatagagg gaaaatattt tttgctactg tggtttttgc
     1441 gcctgctgtt ggccttttga catctcggca agccactctt cctgtgcctt ttgcatctct
     1501 tttgaagaga ggttgcgctt gcgagatgca aatgaccaga cgtgtccata aggatcgatc
     1561 aattgcccgg tcgtgtcacc ccagaatgcg tccattactg gcattgtaac tgttgcacct
     1621 gcggccggtg cttgggtgaa tatcttttcg acatctttga catagacgta taatgctgtg
     1681 cttgctcctc ccaccgactg tggagacttg cagttcatct ctggaaactc gtcgtttagc
     1741 atgatacgag agtcgccaat ctggatctca gcgtgcaata ttatgttgcc acctggcata
     1801 tacgcacgat ggagctcttt ggcaccaaaa gcctttttgt aaaactcgat tgcctgttct
     1861 gctcctttga ctatgagaac tggtgtaacg gagtgatagc cttttggaat tgcgctaact
     1921 tttggttttg ctgccttctt tttctctgcc ttcgccacag cattgcaagt aattaaaaga
     1981 gcgtgtatta aagattgcat aacttttctt ttgaatttcg tcaagtgtcg gcgccctgtc
     2041 gtctgcaaat cttctctcat ttggcaagcc ttgtttggtt atgagttgca ttatgagcca
     2101 aaaaagttgt ctttcgtctt tagatatttg agaccctacc taattgctta tggccataat
     2161 ttcttctaga gaaaaatgcg tttaaataag ttcttgaact ttatgtttaa tgttcaaagt
     2221 aacaattaca tgagcattct tagcaaaaat tctatcactg tcactactgc tactgctgct
     2281 attattcttc ttttcgttat aacaggcgta ataccatatg ggtatcatca gcagaaccat
     2341 cttcgcttta ctgcagaggc aggaggagga ggaccatgta tagactatga aacatcagaa
     2401 aacactattg ccattaactg caacgcttcc tttctagatg ttgtccagac cataaatgac
     2461 cctcagatac ttgaagagca agaagaagaa gaagaagaag aaaatggcca atacattcta
     2521 aacgctaacc tagaggtagc tgatggtgtg acctttgcaa tgacttccaa tgaggatggt
     2581 ctgcaatacc taaagattgc tggtgcaaat ggaataatag ttcatg
//
LOCUS       DASQTF010000123         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_590665,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000123 DASQTF010000000
VERSION     DASQTF010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590665"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VE308_02890"
     CDS             <1..724
                     /locus_tag="VE308_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZA99949.1"
                     /translation="DMIKGTEIKFGRAPRKDELESDFDIIIDSTGFHRSYLPRVGNET
                     WIPCVQYKVHYQQGKEPFDDFYLRAFPSMSGYFWYFPLGNGQAHIGAGDFSKSHNRFV
                     YEFLKKYDCEVIKKVGRPVRLTPPKNCEPFTDGRKSVGAGESIGTVYPLLGEGIIPST
                     WCAELLVQNLHDMSAYRQAVLQKFKIYSLVFNFIKLKITGKFNMIKHGMDMLKLYRHM
                     KSEEERYGMKIKMSDMLKVAGI"
     gene            758..1219
                     /gene="pfdA"
                     /locus_tag="VE308_02895"
     CDS             758..1219
                     /gene="pfdA"
                     /locus_tag="VE308_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HZA99950.1"
                     /translation="MSAANDQSRQQELAAMEQRINELVQQSRILEAYMNDVMTRQVTV
                     SKLIEEAHLASDTIQNITSESDVESLMPIGIGVYVKTTVPPIKKLLINFDAGVAIEKS
                     REDALNYVETRIKEYEVAARQLEAQRQEIAMRINQLQSQINQMIQAAQQPG"
     gene            1244..2692
                     /gene="ftsY"
                     /locus_tag="VE308_02900"
     CDS             1244..2692
                     /gene="ftsY"
                     /locus_tag="VE308_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZA99951.1"
                     /translation="MFDKLKKAFSRAAKGIGQKELTEKVLDDVLLDLQIALLESDVAQ
                     EVVDDLSANLKKELLGLKLEKEQQATEIVQSKLRTAVAEIFSRTNRFDLIENIKMKKE
                     SRGGPFVIVFLGINGTGKTTTVAKIANLLRKAGFSVVVAAGDTHRAGAIEQLEQHTNR
                     LSLKIVKQRYGADPSSVGRDAYDHAKKNHVDIVLMDTAGRMQTSKNLMDEMGKIVRVV
                     KPDVKLFIGDALAGNDTINQAREFFDYTNFDGAILTKVDADAKGGSAISIAHITSRPI
                     VYIGVGQGYDDIIPFDSNKFIGSLFESEGQVGVSVEEVTSMHKISELTVEKAKSEDAQ
                     TVREEEKDILVLSKPPVKPLSPIDGFALPEEQGVKELAPKPILHQPEPSNVKEQPKEK
                     KSRFGFFGKKRNGEDRSKEAEQEADKNEKHKLEEKAVAEEEKDERARLKQEEGQTSDE
                     ERNLKEDKDTKEQKEEDKVVYLTDEDIEDLLK"
     gene            2664..3623
                     /gene="argF"
                     /locus_tag="VE308_02905"
     CDS             2664..3623
                     /gene="argF"
                     /locus_tag="VE308_02905"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZA99952.1"
                     /translation="MKTLKTSLSKKDILSIADLSTNEISMILMLASKLKREQKSGKSR
                     SFLRGKTLGMIFQKPSTRTRVSFEVGMYQLGGGAVYLGANDIQLSRGETIEDTAKTLS
                     LYLDCIMARVYNHKDLETLAKYASVPVINGLSDSFHPCQILADLMTIEEHKKKLKGLQ
                     LAWLGDGNNVCNDLMIGCAKTGINMNAACPSGYEPFKEIVELAKAEAKKTGVDITITN
                     DSASAVKDADIIATDTFISIGKEGELATREATFLPNYQVNIDIMKLAKRDAIFMHCLP
                     AKRGMEVTADVIDGKWSVVWQEAENRLHTQKALLCLLMKAEAL"
BASE COUNT         1193 a          754 c          930 g          833 t
ORIGIN      
        1 agatatgata aaggggaccg aaataaagtt tggacgggct ccaagaaagg acgagctgga
       61 atctgacttt gatatcatta tcgattcgac tggctttcac cgcagctatc tgccaagagt
      121 tggaaatgag acctggattc cctgcgtgca atacaaagtt cattatcaac aaggcaagga
      181 gccctttgat gatttttatt tgcgagcatt tccatccatg tccggttact tttggtattt
      241 tcctcttggc aatggccagg cacacatagg tgcaggcgac ttctcaaaaa gtcacaatcg
      301 cttcgtatat gaatttttaa aaaagtacga ctgcgaagta ataaaaaagg taggaaggcc
      361 tgtccggttg actccaccta aaaactgcga gccctttact gacggtcgaa agtcggtagg
      421 agcaggcgag tcgataggta ctgtatatcc cctactgggc gagggtataa ttccatcgac
      481 ttggtgcgcc gaactccttg ttcaaaacct tcatgatatg tcagcataca ggcaggcagt
      541 gctacaaaaa ttcaaaatat attctcttgt cttcaacttc atcaagctga aaatcacagg
      601 caagttcaac atgataaagc atgggatgga catgcttaaa ttataccgcc atatgaagag
      661 cgaagaagag cgctatggca tgaagatcaa gatgagtgat atgctaaagg tagcaggaat
      721 ctaaagcttt aaaaatctat caagagcagt gaagttaatg agcgcagcta atgatcaatc
      781 gcggcagcag gaattggctg cgatggagca gaggattaat gaattggtcc agcagtcaag
      841 gattcttgaa gcatatatga atgacgtcat gacacgacaa gtgacagtga gcaagttaat
      901 tgaagaggcc catttggctt cagacacgat tcaaaacatc acttctgaat ctgacgtcga
      961 gtcactaatg cctatcggca tcggcgttta tgtaaagacc actgtacctc ctataaagaa
     1021 gttactcata aacttcgatg ctggcgtcgc cattgaaaag agtagagaag acgccctcaa
     1081 ttacgtggaa actaggataa aagagtacga agtagcagct aggcagcttg aggctcagag
     1141 acaggaaata gcgatgcgta ttaaccagct gcaatcgcag ataaaccaga tgatacaggc
     1201 tgcccagcaa cctggttgat tttcgttctg gttgaaaata atcatgtttg acaaactgaa
     1261 aaaggccttt tctagagctg cgaagggcat aggtcaaaaa gagcttactg aaaaagttct
     1321 tgacgacgtg ctgcttgacc tccagatcgc gcttttggaa agtgacgtgg cgcaggaagt
     1381 agttgacgac ctatctgcaa atctgaaaaa agagctgctt gggctaaagc tggaaaaaga
     1441 gcagcaagca actgagatag tccagtcaaa attgcgaacc gcagtggctg agattttttc
     1501 acggaccaac aggtttgact tgattgaaaa cataaagatg aaaaaggaat ccaggggtgg
     1561 accatttgtc attgttttcc taggcattaa cggaacaggc aaaacaacca ctgttgccaa
     1621 gatagcaaac ctcctgcgaa aagctggctt ttcagtggta gtagcggcag gtgacaccca
     1681 tagagcaggc gctatcgagc agctcgaaca gcatacaaat aggttatctc ttaagatagt
     1741 aaaacagaga tatggagccg acccctcgtc agtggggcgt gatgcatacg atcacgcgaa
     1801 aaagaaccac gtcgacattg ttcttatgga cactgctggc cgtatgcaga cttcaaagaa
     1861 cttaatggat gaaatgggca agatagtaag agtcgtcaag cccgatgtaa aattgttcat
     1921 cggcgacgca cttgctggca atgacacaat aaaccaagca agggagtttt tcgactatac
     1981 caattttgac ggtgccatcc taaccaaggt agatgcagat gcgaagggag gctcagcgat
     2041 ttcaattgcg cacattactt caaggccaat tgtatacatc ggtgtcgggc agggctatga
     2101 cgatattatc ccttttgact ctaataagtt catcgggtcg ctatttgaaa gcgaggggca
     2161 ggtaggcgta agcgttgagg aagtgacatc gatgcataaa atctcagaac ttacggtgga
     2221 aaaagccaag tcagaagatg cacagaccgt cagggaagag gaaaaagaca tactagtgct
     2281 gtccaagcct cccgtcaagc cattgagccc tatagatgga tttgctcttc ctgaggagca
     2341 aggggtcaag gaacttgctc caaaacctat tctgcatcag cctgagccat ccaacgttaa
     2401 ggagcagcca aaggaaaaaa agagcagatt tggattcttt ggcaagaaaa ggaatggcga
     2461 ggaccgcagc aaggaagcgg agcaagaggc cgacaagaac gaaaagcata agctagagga
     2521 aaaagcggta gcagaggaag aaaaagatga gagggcaagg ctgaagcaag aagagggaca
     2581 gacgagcgat gaggaaagga acctcaaaga agataaggac actaaagagc aaaaagaaga
     2641 agacaaggta gtctacctaa cagatgaaga cattgaagac ctccttaagt aagaaagaca
     2701 tcttaagcat cgcagatctg agcacaaatg agatcagtat gatcctgatg cttgcaagca
     2761 agctcaagcg tgagcaaaag agtggcaaat ctcggtcttt tttgcgcgga aaaacgcttg
     2821 gcatgatctt ccaaaaaccg tcgactcgaa ctagagtcag ttttgaagtt ggcatgtatc
     2881 agctaggcgg aggtgccgtt tatcttggtg caaacgacat ccaactatcg cgaggtgaaa
     2941 cgatcgaaga tacagccaaa accctttcat tatatttgga ttgcataatg gcacgagtct
     3001 ataaccacaa agatcttgag accttggcta agtatgcttc agtgccagta ataaatgggc
     3061 tttcagactc atttcaccca tgccagattc tggccgacct tatgacaata gaggagcaca
     3121 agaaaaagct aaaggggctg caacttgctt ggcttggaga tgggaacaac gtatgcaacg
     3181 atcttatgat tggttgcgcc aagaccggca taaacatgaa tgccgcctgt ccgagcggat
     3241 acgaaccatt taaagaaatt gtcgagctcg ccaaggccga agccaagaag acaggcgtag
     3301 atattacgat aactaacgat tcagcttcgg cggtaaaaga cgccgacatt atagccacag
     3361 acacttttat ctcgattggt aaggaaggtg agcttgcaac aagggaggcg acctttcttc
     3421 caaattatca agtgaatata gacattatga agcttgcgaa aagggatgca atattcatgc
     3481 actgtttgcc ggcgaagcgc ggcatggaag taactgccga tgtcattgac ggcaagtggt
     3541 cggtagtatg gcaagaagcg gaaaacaggc tgcatacaca aaaggcgctc ctttgcctgc
     3601 tcatgaaagc agaagcattg tagtcattgt tttgcagaag ctcttttgcg cctgctgtac
     3661 cagaaaccaa cgcctgtgat tattgcggca ataactatga tggtgacaat
//
LOCUS       DASQTF010000124        13701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1229946,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000124 DASQTF010000000
VERSION     DASQTF010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229946"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(569..766)
                     /locus_tag="VE308_02910"
     CDS             complement(569..766)
                     /locus_tag="VE308_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99953.1"
                     /translation="MTDQKQQQQHLERNKENVMSFYDLMFNQNNPAEAIKRYVGEVYV
                     QHNPAVADGKEAFIEYFERMA"
     gene            complement(1271..2392)
                     /locus_tag="VE308_02915"
     CDS             complement(1271..2392)
                     /locus_tag="VE308_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA99954.1"
                     /translation="MGRLFGRDSKREDRVPSEERLESDQDESDFQKRDLYNKGVNAMS
                     NENFLDAIRSFDLALRIDPQYVDAWIKRGYAHFHLDEYTVAIASYDRALEVDVNNAET
                     WNLKGLAYYKMKNYDKAIECCEKSVDINPNEGMSWYNKACYLSLSGRIDDGLDALKRS
                     IEIDIQNARKAVRDRDFENARAEEGFRRIIEVVVLESIRQGYDYVGKIVWVTAMDRAE
                     VEDALMRLAMKGLVIKRERRTFTGKEEYYELPSEIANKVGVTKKTGLFGSKQVSAPFQ
                     QLKDIKEVLTKAKDSIEKGDLASTLEHFDELVSPTKHGNAMMEQFFDEHRDLRLYKIR
                     LQDRGQEYLNAHKSDLIDLITKIDHTLGTGVTSKSPAKD"
     gene            2901..5861
                     /locus_tag="VE308_02920"
     CDS             2901..5861
                     /locus_tag="VE308_02920"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZA99955.1"
                     /translation="MMAKPLTAILAAALLVSASGTVGSFYFVSSQGQTTTEPAELTLS
                     ESEASPGSAVEVEGINFGANSQVSIYFMSSEPVDLTDGSAFALQGIDANQSTTTEPEG
                     TSIFFDVDNAAAAALHTLGNLLESSTDQGANDTTTNATITTNAASISEHNLLVALEEG
                     GPVNGTMSLECEDEAIAQGRINGSIGTFGVSPGTYNECSISISDADITDTGEIDELMV
                     LSDSEEDYENSLVTSETADEEGTFESSVIVPNVEAGEYAILAVADDRRTAVSALSVTV
                     AESVTEPVANATEGITNETIIPAPEEEEELAEEESPELLTAEVISNGTQGVAPATFEF
                     QANLTGGTEPYTNIWNFGEGSEESNEQTVLHTFEEAGTYNVTLVATDTDNQNASDSIE
                     INVEAAAAAAPPPTAITDPLIVGIISNGTQDVAPATFEFEVNITGGTEPYTINWNFDD
                     DSEGNDEQNVLHTFEEAGTHNVVVTVIDENRQEASDNLEINVEEPAEEEGGEAATTTL
                     QNQTQNNQTDIAGEEGPTVQLDETSAEPGSPLAISGEGFQPDTPIQIFINNVPITNVI
                     TNVEGSFNTVVIVPTTVAAGSAQVVVRTEQTNIIQTVNIIQLDERTEGPSTLRLTAVS
                     ATDNGEALRNAPVTIIDTSNGQVIETGRTPIDIELQEGTYSIFYSDFGRFEFESAEPG
                     RWIDTPDGGSGLITIREGRNTTVTALYSERPAPPPPPRVTDNSLTLRAQDPQGNPIQG
                     MFVTIYNAEAAERIEQGFTELRVDDLPPGTYPIFFSNFEDLQFLSASPGTWIQTPFGG
                     VGLVTIPDDGEDHNIVVTAEYNRTTVVVEEHFNIQAPLDLEGDIFTITSNQTRPEGPF
                     VMSGSFALRVSDEEPVSATLSAYFMSAREDSNENVDLESQRSRDHDAFQIVDLKPRVA
                     RPIGLNSYVVSGTADLLLNGDMYSNDERIVVIVRGGEELMPTNIEIDFRGDQRYSAAN
                     RLDTLYGVASSGFQ"
     gene            complement(6298..6666)
                     /locus_tag="VE308_02925"
     CDS             complement(6298..6666)
                     /locus_tag="VE308_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99956.1"
                     /translation="MAENRKKVLLNFELKCSDQGQKRIIKAMADFNPEIPNSLITNYS
                     AEYDESESVNIIQQLSDGGIQVAKTGKAFELQLEADNFEFTLNIKPKYQRHWGQSFVE
                     KMESARKKMGYGIATGSDVG"
     gene            6998..7135
                     /locus_tag="VE308_02930"
     CDS             6998..7135
                     /locus_tag="VE308_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99957.1"
                     /translation="MRVVDTMCEWTHEEIKEKETVSAKAKAKDIETFEKKEERPLTAI
                     S"
     gene            complement(7239..8858)
                     /gene="uvrC"
                     /locus_tag="VE308_02935"
     CDS             complement(7239..8858)
                     /gene="uvrC"
                     /locus_tag="VE308_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZA99958.1"
                     /translation="MTFTTTILKEKRIPEQPGVYLMKNSEGVIIYIGKAKNLRKRITS
                     YFSRHDPKLGWKTNRLVSRISDIDFVVTDNEIEAFLLESNLIKRYRPVFNIELKDQQR
                     YTYLKITDEAFPRLLVARRNRAGEFTGPKGRIYGPFVRGSSKYLTVGLLRKLFKVRVC
                     NRLPKTPCLQYFIKNCDAPCIANVMQEQYMMNIVALQDILEGKSSIDKFAEEMQAEMK
                     HASELGDYERAKEIRDTLYRLNNLRIKQKMEKAVNRNHDEEYVSILKDTKKGVAHVMA
                     LRRTRGVISDRKKFEFDLVGDNSLSSFLLQYYSTVPMIPRFVYVNEDLEDKQVLEMSL
                     EKIAEHKVSIFNVEKTLTKDKRQLMGLIIRNLSIYIERGYDPAVVELKNALKLTSMPE
                     VIDCFDVSNLGISITVGSCVRFINGKPSKNYYRRFNIRTVVGQNDFAMMGELVRRRYS
                     SAGEKIPDLILIDGGRGQLGAAIMSLRSLGISDVPCISLAKENEEIYVPSSKDPVALP
                     KSNAGLKMLQYARDEAHRFSLAYNIKLRKLH"
     gene            complement(8855..11728)
                     /gene="uvrA"
                     /locus_tag="VE308_02940"
     CDS             complement(8855..11728)
                     /gene="uvrA"
                     /locus_tag="VE308_02940"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZA99959.1"
                     /translation="MHNKIIIKGARQHNLKNINVEIPKNKLVVITGLSGSGKSTLAFD
                     TIYAEGQRRYVESLSAYARQFLELMDKPDVDSIDGLSPAISIQQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARIGVPHCPKCGRKIASQSADSITETIMKTAESKNVMILGPVIQAKK
                     GTYEKLFYQLKQDGFSRIRLDGEVTRLDDEEEEEEEKSNYPRLDRQKKHTIEVIVDRL
                     KPSPEQRSRLFDSIQSALKVGGGVVVVSVDNKDTMYSQRNACPHCGISLGDLEPRTFS
                     FNSPFGACRECNGLGIKIEFDPDLIIPDKSKSIIEGAIRPWTGHFATFRSAMLRDVGR
                     KFGFDLATPIANMRPEQVRVILYGSDESIHYKYQSRYSDSRWEYRGSFEGVIPNLDRI
                     YRETESESKREDLMQFMRERPCELCNGRRLRDEALAVRIDNRSIMDVCDMSIDECYRF
                     FQTLELTETERYIARTILKEIMSRLEFLLNVGLNYLTLNRMTATLSGGESQRIRLATQ
                     IGSNLTGVLYVLDEPTIGLHQRDNMRLIDTLKKLRDLDNTLIVVEHDEEVIRSSDWII
                     DIGQGAGNHGGAIVASGPLDEIVNNPSSITGAFLRGDQRVVHRHERRNPSKWLTVHNA
                     CENNLKSIDVRFPLGCMTAVTGVSGSGKSTLVNDILFRALATHFYGSKERAGKHDKIS
                     GLEHIDKVIGIDQSPIGRTPRSNAATYVNAFTPIRELFAKTSQAREMGYKAGRFSFNV
                     SEGRCDSCEGGGSKKIEMQFLPDIYVTCDECKGRRYNSETLAIKYKGKTISDVLDMTI
                     EEALKFFYAIPTITKKLQTLSDVGLGYLRLGQPATTLSGGEAQRIKLAAELSKRDTGK
                     TVYILDEPTTGLHFADVSKLLNVLKRLVDLGNTIIIIEHNLDVIASTDWIIDLGPEGG
                     AAGGQIVVAGTPEEVSENMNSYTGEYLKPKLSVDSMILQRANQ"
     gene            complement(11728..>13701)
                     /gene="uvrB"
                     /locus_tag="VE308_02945"
     CDS             complement(11728..>13701)
                     /gene="uvrB"
                     /locus_tag="VE308_02945"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZA99960.1"
                     /translation="NSTGFNLAKDAFPPSGDQPEAIEQLVRGVSKGGRQTLLGVTGSG
                     KTFTIANVIANANKNTLVISHNKTLAAQLYAEFKEFFPKNNVGYFVSFYDYYQPESYI
                     PQTDTYIEKDTEVNEKIEKMRLEATAMLMSGEPTIIVSTVSCIYSLGSPKEWEKNAIA
                     VSRGMTIDRKLLIHNFIEARYERNDISLVPGNLRVKGDTIDIIPGYSDDIIRISMFGD
                     EVEHISIHDHVTMKKLRDVNAVNIFPAKHYITDDDSRSRAIGSIKRELGNWLPNLPSE
                     LERQRLIQRTKYDLEMIEEFGYCSGIENYSRHLDGRAPGQPPYCLLDFFGDDFLLVID
                     ESHVTLPQLHGMYNGDYSRKKMLIDYGFRLPSAYDNRPLKFGEFEKNYLKNVIFVSAT
                     PGPYELKTSLQVIEQLVRPTGLVDPKVEIRPTKNQMEDLIKEIKNRAKKNQRTLITTM
                     TKRMAEDLAEFLAKNKVRVRYLHSEIEGLERTELIRQLRLGEFDSLVGINLLREGLDI
                     PEVSLVAILDADKEGFLRNTTSLIQTIGRAARNLDGTVIMYSDHITQSMKQALEETDR
                     RRKKQMEYNRRYKITPKTIVKSIPESSKEFGDVEVETKSMAKEDLFKLAVETEATMKR
                     FAEELEFEKAIMFREKLDKIRKALGEPAFSKVS"
BASE COUNT         3628 a         3170 c         2843 g         4060 t
ORIGIN      
        1 tcagaatccg tatactctct cattgtttca ttgaacattt ccgctatagt tctggtgatt
       61 tctttctcgc ttacatcggc aaatatcttt gccatatgag atatcatcca cctctgaagg
      121 ttattaatag attaggagtt cctatgcgat ggctataagc taccactgga ctatgacgga
      181 ggcggtttat aattactaat agacaatcag agtcagtcat tgtcgcaatg ctattatcac
      241 cttctgtagt ttccttaata actttctaac tttctaccct ttggcgtcag atggttgaca
      301 accagatacc tctccttacc tcggacggct gcatgttacc accaaccctc gatttgaatc
      361 agaacattgt attatcgtta tcgcttttct caggtatcct ctgtagcaca tcccaatgct
      421 caataatctt tccattgtcg tctaatcgaa aaatgtcaat tcccgcccaa ttacttatgt
      481 caccaggcca ctcctgatag cagtgcaaga ctacatagtt tccttctgct atcgcccgct
      541 tgaagtgaac acgcttgccg ggatactctt atgccatcct ctcaaaatac tcaatgaaag
      601 cctccttgcc gtcggccact gctggattgt gttgaacgta aacctctccg acatacctct
      661 tgatggcttc ggcaggatta ttctggttga acatcaagtc gtagaaggac attacgttct
      721 ctttgttccg ttcaagatgt tgctgttgct gtttttggtc tgtcacaact ttcacttcct
      781 cttattgtta ttggatactt cttaatagtt aatgattaag acgctttata atcacctgag
      841 ttggttattg gtagattgcc agctagccaa gtgtcttgac agaattggcg tatagaagca
      901 ttcgagattt aactaactgg ctggcctatc taattaagtc gtatatgtgc acttatcgct
      961 tttgatgcct tagagattat gtgttatcat agaatatgta cgcgatgggg tatttcgtgc
     1021 acctgtagtc ttcgcaccat catttcgtcc tctactactg tatttcatag ggacattaca
     1081 gtcgctacag attcttttct ctgtcatgaa aaatcatatg tgatagcacg ctttgaataa
     1141 aaatcctagg atgcctgcac ttgtgaaagt catcctagta attgcgggct tgttagtcgc
     1201 ttgcagaaga aaaaataaca aacatcccac taggacgctt tacatgtata tggtataata
     1261 catcgaaaag ctaatctttt gcaggtgatt tgctagtcac gccagttccg agtgtatggt
     1321 ctatctttgt tatcagatca ataagatcag acttgtgagc gttaaggtat tcctgtcccc
     1381 tgtcctgaag ccttatcttg taaagcctaa gatccctgtg ttcatcaaag aactgctcca
     1441 tcatggcgtt tccgtgcttt gtcgggctta caagctcgtc aaagtgttcc agcgtgctag
     1501 ctaaatcgcc tttttcaatt gaatccttag ccttggtgag gacttctttt atatccttta
     1561 gctgttggaa cggagctgaa acttgcttgg agccaaaaag gccggttttc tttgtcactc
     1621 ctaccttgtt tgcaatctca cttggaagtt cataatattc ctccttgcca gtaaaggtcc
     1681 tcctttcacg cttgatgaca agacctttca tagcaaggcg catgagcgca tcctcaactt
     1741 ccgccctgtc catggcagtg acccatacta tcttgcccac atagtcataa ccttgtctta
     1801 tcgactcaag cacgactact tcgatgatcc tcctgaaacc ctcttctgca cgtgcatttt
     1861 caaagtccct atcacgcact gccttgcggg cgttctgaat gtctatctct atagatctct
     1921 tgagtgcgtc aaggccatcg tcaatccttc cgctgaggct aaggtagcaa gccttgttgt
     1981 accaggacat accctcgttt ggattgatgt ctactgactt ttcacagcat tcaatggctt
     2041 tgtcgtagtt tttcatctta tagtaagcaa gtcccttaag gttccacgtt tcagcgttat
     2101 tgacgtctac ctcaagcgcc ctgtcataag aagcgatagc aacagtatat tcatctaggt
     2161 gaaaatgggc atatcctctt ttaatccacg catcaacata ctgcgggtca atccttagtg
     2221 caagatcaaa gctccttatc gcgtcaagga agttttcatt agacatagcg ttgacaccct
     2281 tgttatagag gtctcgcttt tgaaaatcgc tctcatcttg atcggattcc agtctctctt
     2341 cagaagggac acggtcctct ctcttgctat cccttccaaa caatctgccc attaaaataa
     2401 atttccaatt tcacagataa atagattatg ataagaaaag tgctacaatt catgaggctt
     2461 ttatgatctc tttgacatat tttttccata tgagcaatgg tagcggcaac aacaagccca
     2521 atgaaatcta agttgagata tgttgggctc gtccgtgccg taaatgacac cctgtagtcc
     2581 aagcatgagg acgactggat tgtaaaagtc cagcctagct tgactgggtt ggagtagcgg
     2641 ctacaaatta ccagaaccct aacggctcat tgtattgtgc ctgaatagtt ttccaaaagc
     2701 tgtcctccct tacgcgcaag gaagttcata ttccctctca cttcaccact acaaccttgt
     2761 aataaggaac cagcataaga ggccggatct ggacgataac aacgagtagt ctcagagcca
     2821 ccttgactaa acgcagttac atcttcttat agaggtagaa tggtctctct ttttatctga
     2881 taactacgca gcttggctga atgatggcaa agcctttgac tgctattcta gcagcagcct
     2941 tactcgttag tgcttctgga actgtcggct cattttattt tgtaagctcc caaggacaga
     3001 caacaacaga gccggcagag cttactctca gcgaaagtga agcctcgccc ggttccgcag
     3061 ttgaagtaga gggcatcaat tttggtgcta attctcaggt ttcgatttat tttatgtcat
     3121 ctgaaccggt tgatctcaca gatggaagtg catttgctct gcaagggatc gatgcaaacc
     3181 agagtacaac gactgaacca gaagggacta gcatcttttt tgatgttgat aatgctgctg
     3241 ctgctgctct acatacattg gggaatttac tcgaatcgag cactgaccaa ggcgccaacg
     3301 atactactac taatgctaca attactacta atgctgcaag catttctgag cacaacctgc
     3361 ttgttgcact tgaagaaggc gggccagtga atggcactat gagccttgaa tgtgaagacg
     3421 aggccattgc tcaaggtcgc ataaatggca gcatcggcac ctttggagta agtccgggaa
     3481 catacaatga atgctcaatt tctatctctg atgccgatat tacagataca ggagaaattg
     3541 acgagctgat ggttctttct gactctgaag aagactatga aaatagccta gtgacctctg
     3601 aaactgctga cgaagaaggg acatttgaat catcagtaat agtgcccaat gttgaagcag
     3661 gtgaatatgc aattcttgct gtcgcggacg acagaagaac ggccgtttct gcattatcag
     3721 tgactgttgc agaatctgta actgaacctg ttgctaatgc aacagaaggc atcacgaatg
     3781 aaactattat tccagctcct gaagaagaag aagaactagc ggaagaggaa tctcctgaac
     3841 ttctgacagc tgaagtcatt tcaaatggga cacaaggtgt tgcaccggca acttttgaat
     3901 ttcaagcaaa cctaacaggt ggcactgagc cctacactaa tatctggaat ttcggtgaag
     3961 ggagcgaaga aagtaacgaa caaactgttt tgcacacatt tgaggaagct ggcacctata
     4021 acgttactct tgttgctaca gacactgaca atcaaaatgc atctgatagt atagaaatca
     4081 atgttgaagc agcagcagca gcagcacccc caccaacagc aataaccgat cctcttatag
     4141 ttggaatcat ttcaaatggg acacaagatg ttgcaccagc cacgtttgag tttgaagtaa
     4201 atataacagg cggaacagaa ccctatacca ttaactggaa ttttgatgat gacagtgaag
     4261 gaaatgatga gcaaaatgtt ttgcacacat ttgaggaagc tggcactcat aacgtcgttg
     4321 ttactgttat agatgaaaat agacaggaag catctgataa tttagaaatc aatgttgaag
     4381 aaccggcaga agaagaagga ggagaagcag caacaactac gttacagaac cagacacaaa
     4441 acaatcagac tgacatagcc ggagaggaag ggccaacagt tcagctagat gaaacaagcg
     4501 cagaacctgg ctcgccgctt gcgatcagcg gtgagggttt ccagccagac acaccaatcc
     4561 agatattcat aaacaatgtc ccaataacta acgtaataac aaatgtagaa gggtcattta
     4621 acacagtagt catagtgccg accacggtgg ctgcgggcag tgcccaagta gtcgtcagaa
     4681 ccgagcagac caacatcatc caaaccgtca acataataca attggacgag agaactgaag
     4741 gaccttcaac gcttcgctta actgcagtct ctgcaacaga caatggggag gctctacgca
     4801 atgcaccagt cactataatt gatactagca acggtcaggt aatagaaacc ggcaggactc
     4861 ctatcgatat agagcttcaa gaaggcacat actctatatt ctattctgac tttgggcgct
     4921 ttgaatttga gtcagcagag ccaggaagat ggatagatac acctgacggt ggctctgggt
     4981 tgataacgat cagagaaggt aggaacacca ccgtgactgc actatatagc gagcgaccgg
     5041 cccctcctcc gcctccaagg gtgacggata attcgcttac acttcgtgca caagaccccc
     5101 aagggaaccc gatacagggc atgtttgtaa cgatctacaa tgcagaagct gcagaaagaa
     5161 tagagcaggg cttcacagaa cttagagtag atgatctgcc gccagggacc taccctatat
     5221 tcttctcaaa ttttgaagat cttcaattcc taagcgcctc ccctggcacg tggatccaaa
     5281 ctccattcgg aggggtgggt cttgtgacta tacctgacga cggcgaagac cataacattg
     5341 tagtgacagc agagtacaat agaactacag tagtagttga agagcacttc aacatacagg
     5401 cgccgctaga tctcgagggg gacatattca ctataacatc aaatcagacg cgccccgaag
     5461 gaccatttgt gatgtccgga tcatttgcac tcagggtgag tgacgaggag cctgtcagcg
     5521 cgactctctc tgcctacttc atgtccgcaa gggaagattc aaacgaaaac gtagatctag
     5581 agtcacagag gagcagagac catgatgcgt tccagatagt ggacctcaag ccgagggtgg
     5641 caaggcccat tgggctaaac agctatgtcg tttcaggaac cgccgactta ttgctcaatg
     5701 gtgacatgta ttctaacgac gaaaggatag tcgtgatagt gagaggagga gaggagctta
     5761 tgccaaccaa catcgaaatc gacttccgcg gtgatcaacg ctactctgca gccaacaggc
     5821 ttgacacact gtatggtgtc gcgagttctg ggttccaata gtgcgcaaaa gcaggcgatt
     5881 tttttgcgcc ttcaattttt atttatgttt ctgtatccta agaaagacac gtaacagtct
     5941 cttatacaac aatcatgttt tgataaattc aatgaaaaaa tcttgcttcc ttataatggc
     6001 attcaaagtc gatatcggct acctatgttt gcctccaatt tattgtttct aactgcaaga
     6061 ttacgtgttt taagctaatg gcagttgcta tttctggaat gcaagaagtg tctggaatta
     6121 gttcagagag cactacttga atgtatacat atatcatata cttggaaaaa gcctagcgcg
     6181 gtcagaaata gaaagcacgc atttaatgtc taaaaaactc cctattgtgt gtcttgttgt
     6241 ttgatgtgat gctcaaaatc gcaagcgacc ataaaagtcc ttcagccatg ctttaatcta
     6301 gccgacatcg cttccagttg ctattccata acccatcttt ttccttgcac tttccatttt
     6361 ttcaacaaag gattgacccc aatggcgctg gtacttcggt ttaatattca atgtaaattc
     6421 gaaattgtct gcttccagtt gcaattcaaa ggccttacca gtttttgcca cctgaatccc
     6481 tccatcagac agttgctgaa ttatatttac agactcgctt tcatcgtact ccgctgagta
     6541 atttgtaatg agactatttg gaatctcagg gttaaagtct gccatggcct taatgatacg
     6601 cttctgtcct tgatcgctgc atttaagctc aaagttaagc aaaactttct tcctgttttc
     6661 ggccatattg tcatgtgtta taccacccta aaaaattttt ctatctgaaa tactagtaat
     6721 aacttcgtgt aagatcacgt aagattttgt ttacaaattg taatttatct gtcttatatg
     6781 gcaagagcaa gttaaatatt gtctaatgat aatgatactt actctcaagc aaaggggtag
     6841 agaatatttg tcgttgtgga atgtgacgga catacttttc agctagccac ccttactaaa
     6901 ttaaataaat tgtcatcatt gaattgttat aatactactt agtagaagaa tttgccagat
     6961 aaggattgta tttagcatgt tattcttatc tatcataatg cgcgtagtcg acactatgtg
     7021 tgagtggaca cacgaagaaa taaaagaaaa ggagacagtc agcgcaaaag cgaaggcaaa
     7081 agacatcgag acttttgaaa agaaagaaga gagaccactg actgcaatca gttaagacaa
     7141 aaaacataca aacaaaaaca actaactact cttctttttt ctttatctca atatgtgaag
     7201 ttatcacaga cacgcagtat atctctacgt ggaaatgatt aatgcaactt tctcagcttg
     7261 atgttatagg caaggctgaa cctgtgggct tcgtctcgag catactgcag cattttgagg
     7321 cctgcattac tcttcggtag cgcaactggg tctttggaac taggcacata gatctcttca
     7381 ttttcctttg caagactgat gcaagggaca tctgagattc ctaagcttcg aagtgacatg
     7441 atagcggctc caagctgacc gcgcccaccg tctatcagta taagatcggg aatcttttcg
     7501 cctgcagacg aataacgccg gcgaacaagc tcgcccatca tcgcaaagtc attctgccct
     7561 actactgttc tgatgttgaa tctcctgtag tagttcttac taggcttgcc gttgataaac
     7621 cgaacacatg aaccaacagt aattgagatt ccaaggtttg acacgtcaaa acaatcaatg
     7681 acttcaggca tggaagtaag ctttaaggca tttttaagct ctaccactgc tggatcatag
     7741 cccctttcaa tgtatattga aaggttgcga atgataaggc ccataagctg ccgcttatcc
     7801 tttgtcagtg tcttttcgac attaaagata ctcaccttgt gctcagctat tttttcaagt
     7861 gacatctcta acacctgctt gtcctcaagg tcttcgttga catagacgaa ccttgggatc
     7921 attggaactg tagagtagta ttgtaaaaga aatgaggaaa gcgagttgtc gcctactaga
     7981 tcaaattcaa attttttccg atcgcttatg acaccacgtg tcctgcggag tgccatcacg
     8041 tgggctaccc cctttttggt atcttttaaa atactgacat actcttcgtc atggttgcgg
     8101 ttgaccgcct tttccatctt ttgcttaatg cgcagattgt tcagtctgta cagagtatcc
     8161 cggatctctt ttgccctttc ataatcgcca agctcggatg catgcttcat ctctgcctgc
     8221 atttcctcag caaacttgtc gatactgctc ttaccttcaa gtatgtcttg caatgctact
     8281 atattcatca tatattgctc ctgcattaca ttagctatgc atggcgcatc acaattcttg
     8341 ataaagtact gaagacaggg agtcttgggc agcctattgc atactcttac cttgaataat
     8401 tttcgcaaga gtccaactgt caagtattta gaactgcctc gcacaaacgg tccatagatt
     8461 ctgccttttg gtccggtgaa ttctcctgcc ctgttgcgtc tggcgacaag gagacgggga
     8521 aatgcctcgt ctgtaatttt tagataagta tagcgttgct gatccttcaa ttcaatgttg
     8581 aagaccggtc tgtaacgctt aatgagattg gactcaagaa ggaatgcctc aatttcattg
     8641 tcagtaacga caaaatctat gtctgatatc ctgctaacca gcctgttagt cttccatcct
     8701 agcttggggt cgtgcctact gaaataagag gtgatacgtt ttcgcaggtt tttggccttg
     8761 ccgatgtaga tgatcacacc ttcactattc ttcattaggt agacgcctgg ttgttctgga
     8821 atgcgctttt cttttagaat cgtagtagtg aaagtcattg attggccctc tgcaatatca
     8881 tactatcaac tgacagcttt ggcttcaaat actcgccagt ataactgttc atgttttcgc
     8941 ttacttcctc tggggtacct gctacaacaa tttgcccacc tgctgcgcca ccttctggcc
     9001 ccaggtctat tatccaatca gtcgacgcaa ttacatctag gttgtgctcg atgattatga
     9061 ttgtgttgcc aagatcaact agccttttta acacatttag cagcttgctt acatctgcaa
     9121 agtggaggcc tgtagtaggc tcgtctaaaa tgtagactgt cttgccagtg tcgcgctttg
     9181 acagctctgc cgcaagcttg attctctgag cttcacctcc tgagagtgta gttgcaggct
     9241 gccccagtct caggtagcca agacccacat cgcttagtgt ctggagcttt ttggtaatag
     9301 tcggtatcgc ataaaagaat tttagtgcct cttcgatagt catatcaagc acatctgaaa
     9361 tagttttgcc cttgtactta attgcaagtg tttcagaatt atagcgtcta cccttgcatt
     9421 cgtcgcatgt gacatagata tcgggcaaga attgcatctc tatctttttt gagccgccgc
     9481 cttcacacga gtcacatctc ccctctgaga cattaaatga gaacctgcca gctttatatc
     9541 ccatctctct tgcctgagat gtcttggcaa atagctcccg tatgggcgta aaggcattga
     9601 cataagtggc agcatttgat cttggagttc tgccaattgg agattgatca atgcctatca
     9661 ccttatctat gtgctcgagg cctgatatct tatcatgctt acctgctctt tcctttgaac
     9721 cgtaaaaatg tgtggcaagt gctctgaaca atatatcgtt tacgagtgtc gacttgcctg
     9781 agcctgaaac gccggtgaca gctgtcatgc agcctagagg aaacctgaca tcaatagact
     9841 ttagattatt ctcacatgcg ttgtgaaccg tcagccactt gcttggattt ctccgttcat
     9901 gccgatggac aacccgttga tcgcctctga gaaaagcgcc tgtaatggaa ctaggattat
     9961 ttacaatctc atcaagcggg ccacttgcaa caatagctcc tccgtggtta ccggcacctt
    10021 ggcctatatc tattatccaa tcagagcttc gtataacttc ctcatcatgt tctacaacaa
    10081 tgagagtgtt gtctagatct cgaagttttt tgagagtatc tattagccgc atgttatcgc
    10141 gctgatgtag gccgatcgtt ggctcgtcca atacgtagag cacacctgtc aggtttgacc
    10201 cgatctgggt cgcaagccta attctttgag actcgccgcc tgaaagcgtg gcagtcattc
    10261 tattgagagt cagataattg agccctacat ttagtagaaa ttcgagtctt gacatgatct
    10321 ccttcagtat agtccttgcg atgtagcgct cagtttctgt gagctctaga gtttgaaaga
    10381 agcgataaca ctcatctatt gacatgtcgc acacgtccat tattgacctg ttatctatcc
    10441 tgactgccaa agcctcgtca cgcaggcgcc tgccattgca caactcgcag gggcgctccc
    10501 gcatgaattg cattaggtct tcgcgctttg actctgattc ggtttcgcgg taaatgcggt
    10561 caaggtttgg tatgacgccc tcaaatgatc cgcgatattc ccatcggctg tcagaatagc
    10621 gcgactggta cttgtagtga atgctctcgt cactgccata gagtattacc ctaacttgtt
    10681 ctggcctcat gttggctatc ggagtcgcaa ggtcaaaacc aaactttctg cctacgtcgc
    10741 gcagcatcgc agatcgaaat gttgcaaagt gtccagtcca cggccttatt gctccctcta
    10801 ttatgctctt tgacttgtct ggaattatca aatctggatc aaattcaatt ttgatgccaa
    10861 gtccattgca ttccctgcat gctccaaacg gcgaattgaa tgaaaaggtt cggggctcca
    10921 agtctccaag cgaaataccg cagtggggac aggcattcct ctgtgaatac atcgtgtcct
    10981 tgttgtcaac tgaaactaca acaactcctc cgcctacctt caacgcagat tgtatcgagt
    11041 caaagagcct actcctttgt tctggagagg gtttgagtct atctacaatc acttcgatgg
    11101 tgtgcttttt ttgcctgtcg agccttggat aattggattt ttcttcttct tcttcttcct
    11161 catcgtcgag cctcgttacc tctccatcca acctgatgcg agagaagcca tcctgcttca
    11221 actgatagaa taatttttcg tatgttcctt tctttgcctg tatgacaggg cctaagatca
    11281 taacattctt actttctgct gttttcatta tagtttctgt gattgagtca gctgactggc
    11341 tggctatttt tctgccacac ttgggacaat gaggtacgcc tattcttgca aaaagcagac
    11401 gcagataatc gtaaatttct gtcaccgtac caacggtcga gcgggggttc ttgctcgtcg
    11461 tcttttgctg tatggatatt gctggcgaca aaccgtcgat tgaatcaacg tcgggcttgt
    11521 ccatcaattc caaaaattgt cttgcataag ccgataatga ttccacataa cgcctctgac
    11581 cttctgcata tattgtatca aatgcaagtg tcgatttccc agagccagat aggcccgtaa
    11641 tgactaccaa tttgttcttt ggtatttcga cattgatgtt cttgagattg tgctgtcttg
    11701 ctcctttgat tattatcttg ttgtgcatta tgacaccttt gagaatgccg gctcgccaag
    11761 agcctttctt atcttatcca gcttttcgcg aaacattatt gccttttcga actctagctc
    11821 ttcagcaaat ctcttcatgg tggcctctgt ttctacagcg agtttgaaca agtcctcctt
    11881 tgccattgac tttgtctcaa cttcaacatc gccaaattcc ttactacttt ctggtattga
    11941 cttaactatt gtctttggag ttatcttgta tctcctgtta tattccatct gctttttgcg
    12001 tctgcgatca gtttcttcga gggcttgctt catcgactga gtaatatgat ctgaatacat
    12061 tatgacagta ccgtcaaggt ttctcgctgc tcgaccaatc gtctgaataa ggctggttgt
    12121 attacgcaag aatccttcct tgtcagcatc aaggattgca acaagggaaa cttctgggat
    12181 gtcaaggccc tctcttagca agttgatgcc tactagtgaa tcaaactcgc caagacgtag
    12241 ctgccttatg agttctgtac gctcaagacc ctcaatctca gaatggagat aacgcacgcg
    12301 cactttgttt ttcgcaagaa attctgccaa gtcttctgcc attctctttg tcattgtcgt
    12361 gataagcgtc cgctggttct tctttgccct gttctttatc tcctttatca aatcttccat
    12421 ctggttctta gtgggccgga tctcgacctt ggggtccaca agccctgtgg ggcggactag
    12481 ctgctcgatg acttgcaagc tagtctttaa ttcatagggg ccaggtgtag ccgatacaaa
    12541 gatcacattc ttgaggtaat tcttctcaaa ctctccaaac ttgagtggac ggttgtcgta
    12601 tgcacttggc agacgaaaac catagtcaat tagcatcttt ttgcgtgaat agtcgccatt
    12661 gtacatgcca tgcaattgtg gtaatgtaac atgcgactcg tcaattacta acaaaaagtc
    12721 atcaccaaaa aagtcgagca agcagtaagg cggctgtccc ggtgcccttc cgtccaagtg
    12781 acgagagtaa ttctcaattc ccgagcaata gccaaactct tctatcatct ccaaatcgta
    12841 ttttgtacgc tgtataagcc tctgcctttc aagttctgat ggcagattgg gaagccagtt
    12901 gcccaattcg cgcttgattg atccaatagc tctgctacga gagtcgtcgt cagtaatgta
    12961 gtgcttggct ggaaatatat tgactgcatt tacgtctcgc aattttttca tagtaacatg
    13021 gtcatgtatg cttatgtgct caacctcgtc accgaacatt gagatcctga taatgtcatc
    13081 agaatatcca gggatgatat caatagtatc acccttgacg cgaaggttgc cgggcacaag
    13141 tgatatatca tttcgctcat aacgcgcttc aataaaattg tgaattagca atttgcggtc
    13201 gatcgtcatt cctcttgata ctgctattgc atttttctcc cactcttttg gcgagccgag
    13261 ggaatatatg cacgataccg tagacacgat tattgtaggc tcccccgaca ttaacatagc
    13321 agtcgcttct agcctcatct tctctatctt ttcatttacc tcagtgtctt tttcgatata
    13381 ggtgtctgtc tgagggatat aactttcagg ctgataatag tcataaaagc taacgaaata
    13441 gccgacatta ttctttggaa agaattcttt gaattcagca tacagctgcg ctgcaagggt
    13501 cttgttatga gaaatcacaa gggtattctt gtttgcattt gcaatgacat ttgcaattgt
    13561 aaacgtctta ccactgccag tcacgccgag gagtgtctgg cgtccaccct tactcacacc
    13621 tctgacaagc tgctctatcg cttcaggctg gtctccgctg ggaggaaagg catcttttgc
    13681 cagattaaag cctgtagagt t
//
LOCUS       DASQTF010000125         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_950075,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000125 DASQTF010000000
VERSION     DASQTF010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950075"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VE308_02950"
     CDS             <1..324
                     /locus_tag="VE308_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZA99961.1"
                     /translation="KAIEDAPVLIVVCSNTSRSVGRYGQRGRDFYSIMDGAFASMLIL
                     LTAINEEIGAGFVGAFEDDKIAKILELPEYVKPIGIIALGYPDESPARLGRIESEQIV
                     HHERW"
     gene            1752..1988
                     /locus_tag="VE308_02955"
     CDS             1752..1988
                     /locus_tag="VE308_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99962.1"
                     /translation="MSCYILYCSEVVVFVGRTFLYNPLMLFYAIFLGLSGDKENKKSL
                     GSSVASNIRDNETTKETMIGSISKEEEEKEEGER"
     gene            1985..2800
                     /locus_tag="VE308_02960"
     CDS             1985..2800
                     /locus_tag="VE308_02960"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZA99963.1"
                     /translation="MMADRETDSSNSFERTTIKLISTNKTIRLNDAIMVAGFPGPGLV
                     GSMSVSYIIEQQKMHQIGYIDSDFIVPGVIYIGGKLRHPFRIYANDEGTACVLVCDAP
                     VILSGIRSVLNAVVIWAKYNGVREIMVLEGMAAPGISETGRRPWVLSNDGQSDDHGYL
                     SRIKDKKAEASPPAFISGVSGGLLAACLSNRIPCTALLIPSITGIPDPEGAAILIEKT
                     NELANNPFRIDVRQLRSEAEALKRQLQELINSVKRQQQDQQQTQSGGGTGIYS"
BASE COUNT          936 a          598 c          728 g          850 t
ORIGIN      
        1 aaggctattg aagatgcacc agtattaata gtagtctgct caaacacctc tcgctcggta
       61 ggacgctatg gccagcgcgg gagagatttc tatagtatca tggatggggc atttgcgtca
      121 atgctcattt tgctcactgc aataaatgag gaaataggcg cagggtttgt gggcgcattt
      181 gaagatgaca agatagcaaa gatattagag ctgccagaat acgtaaagcc aatcggtata
      241 attgcgctag gatatcctga cgaaagtccc gcaaggctgg gacggatcga gagcgaacag
      301 attgtgcacc atgagcgttg gtaattgata gctaagtaag gtaaagtatt tgttactgcc
      361 attacgccct taataggcag ttgaagtgct ctgactatag acgcttgcta tcacatagtg
      421 aatttatcgc cagaaagtga atattgtcac gtcatatgca gctagccctg tagatgacta
      481 tttggttgtg aatcttatcc tttgcaaaag agagcttcta ctagaagatg aggatagagg
      541 agggtccagc ctcactataa tattttctgc attttagcgc tgcattaact ctggactaat
      601 gggatttctg tgcggcatca ccaccataat cacacattct gcttttcagg catctacctc
      661 taatgaaggt taatttcgat tattcttttt tggttgtagt tatgtgtaca gtattaactt
      721 ctaacttcgc aatcataatt ataatctact aatttattag ttagtccata cagcaatagg
      781 aaaagtgtta tcactgcttt tgtgcacaat attacatgac agaatcatca acaactccgg
      841 caggatacaa ctacacatgc aatgaatgcg gggcttcctt tgaagcgatg gaagatctaa
      901 caaagcactg caggcaggat catccacagt cgaaataaag ttcacaacgg ctagaggggt
      961 tgtagaaatg cattgaagga tctataaagg aaaaataagt gcgggacaac gttcgtgaat
     1021 caagctccta cgatcgagaa tcaaccaaga atgttgataa ggctaattca tagtactgca
     1081 tgtggatcga gctctaatga aagacgacta tcaacttaac tttggacgat cagaaaagtt
     1141 agtagggtct ttggtagaca ggtggagact ttgagattgt aaccgatact atccttattg
     1201 ttcatcacat ttgcacacat gagatatcac cttaggtgca ttggaataaa gttgttgttt
     1261 cgatccaaac acaaaagatc atattacaag ttaatactgt agggtagcaa taggcattgg
     1321 tggcagtatt cagttggatc tcaggcagcc agtcttacca tgcacggcaa gcgtggcatt
     1381 gctcaagtaa gtaagccata attttttaca aagtatcagt accattcacc atgatcgccg
     1441 acctgctatt gacactatcc ggtctgataa gatacaggga gaagaataca agcatatcca
     1501 gttgagctac ttgtgcaatg ctatggcaga ctgatgcatg ttttttattt gatattgtcc
     1561 aaacagacat catgtctgat accggcttta catgtccaaa atgcgatcat tcctttccta
     1621 cggccgagca agttgctgaa catcttagaa acgagcatgt agatggaacg caaactgaat
     1681 ctacaaagcg aatgacataa aattcatcag cagtcaaagc tcctcgcggt tttgcatgct
     1741 ttttaacata tatgtcgtgt tatattctgt actgctcgga ggtagttgtt tttgttggta
     1801 gaaccttctt gtataatcca ttaatgctat tttatgccat tttccttgga ttgagtggag
     1861 ataaagagaa caagaagtcg cttggttcgt cagttgcgag taacatcaga gataatgaaa
     1921 ccaccaagga aactatgata gggtcaattt ccaaagagga agaagaaaaa gaagaaggag
     1981 agagatgatg gcggacagag aaactgacag cagcaacagc tttgaaagaa caaccataaa
     2041 gctcatttcg acaaataaga ctataagatt aaacgatgcg atcatggtcg caggttttcc
     2101 cggaccagga cttgttggct cgatgagcgt aagctacatc atagaacagc agaaaatgca
     2161 ccagatagga tatatcgact cagactttat agttcccggc gtcatctaca taggtggaaa
     2221 gttaaggcat ccatttcgca tctatgcaaa tgatgaaggc acagcatgcg ttcttgtgtg
     2281 tgatgcccca gtaattctat ccggcatccg ctctgtgctc aatgcagtag tcatatgggc
     2341 caaatataat ggcgttagag agatcatggt tttagaaggc atggctgctc cggggatctc
     2401 ggaaacgggt agaaggccct gggttctgtc aaatgacggg caatcggatg atcacggcta
     2461 cctgagtcgc atcaaagaca agaaggcaga agccagcccg cctgcgttta tttcgggagt
     2521 atctggtggc ctcttggcag catgcttatc aaacagaatt ccttgtacgg cactcttgat
     2581 accatcaatt actggaattc cggacccaga gggagcagcc attctgattg aaaaaacaaa
     2641 tgagcttgcc aataatccgt ttagaataga tgtgcgtcag ctgaggagtg aagcagaagc
     2701 gctcaagcgc cagctgcagg agctgatcaa ttctgtaaag aggcaacaac aagatcagca
     2761 acaaacgcag tcgggcggag ggacgggaat atacagttaa ccagttacta tggaatctat
     2821 tcttttgttt ctatgcatgt taaatcgctg ttcatctagc gatttcggtg tttgtactat
     2881 accagttgtt cggactttgt actagtaata ctacgatggc agacggcaga cagcaaagca
     2941 tgttctcagg tgtttgattg gatgcaatat tggtgaaggt attggagcgg caataggctt
     3001 tacgctgggt tgggatatga tttcgacgct tgtattagca ttagggcttg cttttgcaat
     3061 aggctacgct tttacaataa ttctgatgct caaacaatgc cgtttagaca ag
//
LOCUS       DASQTF010000126         8984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1335940,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000126 DASQTF010000000
VERSION     DASQTF010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335940"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2622
                     /locus_tag="VE308_02965"
     CDS             <1..2622
                     /locus_tag="VE308_02965"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HZA99964.1"
                     /translation="GEDKHHGFIFSLLNPPVPLGGNIISLDIYVDELPVQRNSIFIAT
                     SDEVVNVATLSENRPISFKPYQSARFLIIKEGGLDDNVKHKIMIMSKLEGFEEITIPF
                     TFTDYISGYQKDSIHITPDSLASIDSAAVTTGDTMFKNFTSPLVLSGKKAYIVCSANG
                     ALSANWTWMGVRYDNGGLYVPPSRSFGRIIVEVSMDDGVRKRLPNFALSSRHEHGIVT
                     THHDLGGLHVQRRLCVPFENRGFIMEIKMGMRPHTERDLRPAKRKAPRKVRFHFLIDG
                     NITSYGLAAISQSNISKFNKLNRCLQISTARNTKKDKMQYFGTIGVMPKDLKPSKILT
                     DSFDNDLELSYDLELEEGQDINLALVGVGSFSSAEECLSEFCFMRDNYSKLMQDTEDT
                     FKKYSTSTLTFRSPSTEKRQNLQISKLLRAYEKAKTSLQYLKAEYDGLGQGICAGLPR
                     FPNYWARDTGWSLRGYLAVGDYHFARSVIDNFFNHQAKNTTESAVKGELPMIISGKAF
                     LHNTTYGSADSTFLFPWALREYVLATADVEYLQKKWESIEALVDCGLLKDTDGDGLID
                     HGFTGTAEKLPIRDSTWMDHIDRRKSANDVQALFYQSLKIGSELAGIVGDSYNQERWG
                     QEAEQLALKIDHEYWSEKTEYYFDTIRRDGSKDPSIRPNALVLLLTDAVKDPLKTKSV
                     IERIEQEDMTTSWGVRTLSNLDPRYHPSLYHDGAVWPLVTGWAALSEIKAGRSEQALY
                     YVNCMAERILAENGMFAETYKGDKPDPFNSCILQAWSISMYIHALREMMLGMKINLLE
                     NKISFKPQIAGSLLQEYTHFVFEHSINDKGRFQATVDPYNRKVLVTLKDWHGARPEFL
                     VDSSSYNIDVVNTATGG"
     gene            2672..3352
                     /locus_tag="VE308_02970"
     CDS             2672..3352
                     /locus_tag="VE308_02970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HZA99965.1"
                     /translation="MTVINIQGHNVNPALVVIDVQNGFVSKGGSYDQLGMDVSRYRHV
                     IPQISELISLCRSVNIPIFYTQAVREKSGIDLLTNTHRILPKSREERIRTKPICVINT
                     WDADIVDEIKPSPMDHVVIKRRDSAFQDTEIGVWLKSIGIDTLAFCGIDTSICVEASL
                     RDAFNNGYDVILISDATASGNIKHSESTLEVVRDYYGLVMDVKEFARHLPQKAEQATS
                     NLGYKTLV"
     gene            3476..4699
                     /locus_tag="VE308_02975"
     CDS             3476..4699
                     /locus_tag="VE308_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99966.1"
                     /translation="MDKYKKANPRSPYLGIFGAIVLVTLVSSSGLYALTYGVPYQPQQ
                     QSATIIVASANQNTTGLATSPNATSSVANETMTSAATTPNVTFVEFVSNIEQIRGHLD
                     QALVNKESGNDTLAQAHTLHPIEEVYASIEEQLVSQNSALNETLSVALQDLSSSVPNV
                     ALEEFGGQIDSINMQLDNIVQTLVPSSELSNPAFNASVVARLLHIAGHEYEEAVANGT
                     IIEIVEYQDGQAFIHRAQSIFNSSASSINQSMAQEVEEVNELFSNLNNALNNRDDPAN
                     VETTINGIIHELAEITGLSESQLTGEEAGGEEQDPIAIINTIKSMLTQLIAAYGSQDY
                     QGAESIAIEAYLENYEFIEAPIAQRDQQLMEQTEIMLREELRQMITDRVPIEQVEQHI
                     AMINANLDRATELLQ"
     gene            complement(4997..7219)
                     /locus_tag="VE308_02980"
     CDS             complement(4997..7219)
                     /locus_tag="VE308_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HZA99967.1"
                     /translation="MAFAHAFIPHTTTFPSIKDQLGKTAGEQSTKQLESLLTDLPLRM
                     RTGEYSEEKTRESIGQIRILLDQISQQSIGPQFLSDKGMVSQAVVFLLRDAVQSYATT
                     ISNASNGSDSTTIQQAIDYENAVGLVQIAHQNYQKTLSSLIEDNRRRTEIESFFNELE
                     SSLAQKIDNEAILRLTTAIERDLAEELSVSEGSESTSEHQQYFATIRTLLSQVISEVN
                     NGNYEQADQYAISAYLDNYEYLEAPIEKHDPDLMVTIEEEMREELRRMIEARESVESI
                     EAFVNGILIKLDKAEELLKDDASFNQSSSAPTSSSGIFADIEGLKAGVGTYTGERRDI
                     GEAEDPAKAAVRSDIDTIRMQLDETLRLHKEGNYDGALLTSRSAYLDSYESIEIPLRP
                     IDPDFTLDMEIKFAELRNLIQAHTPYEQVEAKIVEIRRGLDESERLVSGTGIVAPTIA
                     FSTSFSIIFREGLESALIIGAILTYLEASRNNRFKKHVYYGIVIAIGATAVTWFIAEF
                     IIDISGASRELIEAIAGVSAVAVLFWVSFWVLNKIETKRWIEFVKAKVWKATTTGSIM
                     VFVLLSFFTVYREGFETVLFYQAMLSFAKYMEWYVVAGLVLGLGVIVATTIVVRKLGR
                     RLPLKVLFALTMGIGAYMSIAFMGNAIREFQEVGYISTTHLIGTVPRLDINLATMTGI
                     HPTVETIVAQIALLAVYVVGSLYILIIRPRRQRLIESARKSREDLVRQDESREEGKSL
                     "
     gene            complement(7764..7931)
                     /locus_tag="VE308_02985"
     CDS             complement(7764..7931)
                     /locus_tag="VE308_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99968.1"
                     /translation="MTMVIKRKDARGEDFEISPLQIDFGNIGNDSETVAYQFSELRPI
                     QNELEKNKKRH"
     gene            complement(7979..8140)
                     /locus_tag="VE308_02990"
     CDS             complement(7979..8140)
                     /locus_tag="VE308_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99969.1"
                     /translation="MRNDTTTFKDGERIAGMKVERLIGDKSLILTKHGRKNTSAFAVN
                     MHRILGIFT"
     gene            complement(8189..8698)
                     /locus_tag="VE308_02995"
     CDS             complement(8189..8698)
                     /locus_tag="VE308_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZA99970.1"
                     /translation="MAKIREASANISDYMSNNNNRIRINQNQEELFIGTAESEHIEMY
                     LKAIWFLHEKGQDAKISSIAKLLNVTQPSVVQMLRKLHNSNLVEYSQTKVALTQDGQR
                     IGRQMIRNTRLLEVMMKDALKIDVDEEMACGIEHHMKNIFTDALCILLKHPRKCPHGH
                     NIPKGKCCN"
BASE COUNT         2617 a         1881 c         1836 g         2650 t
ORIGIN      
        1 ggagaggata agcaccatgg ctttatcttt tcgcttttga atcctccagt tccgcttggc
       61 ggtaacatta tttcacttga catttacgtg gatgagctgc ctgttcagag gaactcgatc
      121 tttattgcaa cttctgacga agtggtcaat gtggcaacac tctctgaaaa caggccgatt
      181 tccttcaaac cgtaccagag tgcaaggttc ttgataatta aagagggtgg cttagacgac
      241 aacgtcaagc acaagataat gataatgagc aagctcgaag gctttgaaga aataactatt
      301 ccatttacat tcaccgacta catctcggga tatcagaagg atagtatcca tattactcca
      361 gattcactgg catccattga ttcagctgct gttacaactg gggacacaat gttcaagaat
      421 ttcacaagcc cgcttgtgct atccgggaaa aaagcctaca ttgtatgctc tgcaaacggg
      481 gcactttctg ctaactggac ttggatgggc gtcaggtatg acaacggagg gctttacgtt
      541 cctccatcaa ggtcctttgg aaggataata gtcgaagtat ctatggacga tggcgtgcgt
      601 aagcgccttc caaattttgc attgtcctct agacacgaac acggcattgt tacgacacac
      661 cacgatctgg gaggcttgca cgtgcaaagg aggctgtgtg taccttttga aaatagagga
      721 ttcataatgg agataaagat gggcatgagg ccacatacag agcgtgatct gaggccggcc
      781 aaaagaaagg caccacgaaa agttcgcttt cactttttaa tagacggcaa catcacaagc
      841 tatgggcttg cagctatttc tcagagtaat atctccaagt tcaacaaact caatagatgt
      901 ctgcagataa gcaccgccag aaataccaag aaagacaaga tgcagtattt tggaaccata
      961 ggtgtcatgc caaaagacct aaagccaagc aaaatcttga cagactcatt cgataacgat
     1021 cttgagctct cttatgatct tgagcttgaa gaaggtcaag acatcaacct tgcacttgta
     1081 ggagtaggca gcttttccag tgccgaagag tgcctaagtg aattttgctt catgagagat
     1141 aactacagca agcttatgca agataccgaa gacactttca aaaaatactc cacctcaacg
     1201 cttacattca gatctccctc aacagaaaaa agacagaatc tccagatttc aaaattgcta
     1261 agagcatatg aaaaggcgaa gacatctttg caatatctta aagcagaata tgatggcctt
     1321 ggacaaggga tatgtgcagg tcttccccgc tttccaaact attgggcaag ggacacgggt
     1381 tggtcactcc gaggttacct tgcagttggg gactatcact ttgctaggtc tgttattgac
     1441 aattttttca accatcaggc gaaaaatact acagagagtg cagtcaaggg cgagcttccc
     1501 atgataataa gcggcaaggc cttcttgcac aacactactt atggctcagc tgactcgaca
     1561 ttcctatttc cgtgggcatt acgggaatat gtactcgcaa ctgcagatgt tgagtacctg
     1621 caaaaaaagt gggaatcaat tgaagcactc gttgattgtg gccttctcaa agatacggac
     1681 ggagatggct tgattgacca cggctttact ggaactgcag aaaagctgcc tatccgagat
     1741 tcaacttgga tggaccatat agacaggagg aaaagcgcaa acgacgttca ggctttgttt
     1801 tatcagagct tgaagatcgg aagcgagcta gcaggcatag ttggtgacag ctacaatcaa
     1861 gagaggtggg ggcaagaggc agagcaacta gcgcttaaaa tagatcatga atactggagt
     1921 gaaaagacgg aatattactt tgacaccatt agaagggacg gatccaaaga tccaagcata
     1981 agaccaaatg cgcttgtgct tttgctcaca gacgctgtaa aagatcctct caagacaaaa
     2041 tcagtgatcg agagaatcga acaagaggat atgacaacaa gctggggtgt gcggacgctc
     2101 agcaacttgg atcctaggta tcatcctagc ctctatcacg acggcgctgt ctggccgctt
     2161 gtcactggct gggctgcatt gtccgagatt aaggctggaa ggagcgagca agcactctac
     2221 tatgtcaatt gcatggcgga gcgcattttg gctgagaatg gcatgtttgc agaaacttac
     2281 aaaggagaca aacctgaccc cttcaattca tgcatcttgc aagcatggtc tattagcatg
     2341 tatattcacg ccttgcgcga aatgatgctt ggaatgaaaa ttaacttact tgaaaacaag
     2401 atctcattca aaccgcaaat agcgggttca cttttgcaag agtataccca ttttgtgttt
     2461 gaacacagca ttaatgacaa aggaaggttc caagctacag tcgacccgta caacagaaaa
     2521 gttcttgtca cattaaagga ttggcacgga gcaaggccgg aatttcttgt cgactcatca
     2581 tcatataaca tcgatgttgt taataccgct acaggtgggt gaatagtatt acttcaaatt
     2641 tattaattca atccgtattt gtgatcccta tatgacagtt atcaacattc aaggacataa
     2701 tgttaatcca gcgcttgtag taatagatgt tcaaaacggc ttcgtaagca aaggtggctc
     2761 atatgaccaa cttggaatgg atgtttcccg ctaccggcat gttatcccac agatatctga
     2821 actcatcagc ctttgcagga gcgtcaatat accaatattt tatactcagg cagtacgcga
     2881 aaaatcaggc atagatcttt taacaaacac tcatagaata cttccaaagt cacgagaaga
     2941 gcgcatacgc acaaaaccaa tctgtgtaat aaacacttgg gatgcagata ttgtcgatga
     3001 aatcaagccg tctccaatgg atcacgttgt catcaagaga cgagattcgg cctttcaaga
     3061 tactgaaatt ggagtatggt tgaaaagtat tggaatcgat actctggcgt tctgtggaat
     3121 agacacctcg atatgcgtag aggcatcttt gagagatgca ttcaataacg gatacgatgt
     3181 catactcatc tcggatgcta cagcatcagg caacattaag cactccgagt ccacgttaga
     3241 agttgttaga gactactacg gcttggtgat ggatgtaaag gagtttgcta ggcatctacc
     3301 tcaaaaagcc gagcaggcaa caagtaattt aggatataaa actcttgttt aatccttcgg
     3361 cgataccctg ctgcgactag cagtgataag gaagaactaa agaagcaatg ttttactatt
     3421 gttttgtttc aaaactctta aaaacttagg ctcgcctaat taggcgaacc taattatgga
     3481 caaatacaaa aaagcaaatc ctagatcacc atatcttgga atctttggag ctattgtcct
     3541 ggtgacgtta gtatcttcat caggcctata tgctcttaca tacggagtgc catatcaacc
     3601 gcaacaacaa tctgctacaa taatagtagc aagtgcaaat cagaatacga caggcctagc
     3661 gactagccct aatgcaacta gcagtgtagc aaatgaaaca atgacatcag cagcaactac
     3721 accaaatgtt acctttgttg aatttgtttc aaacatagag cagataagag gacatctcga
     3781 tcaagcactt gtcaataaag aatctggaaa cgatacccta gctcaggctc acacactaca
     3841 cccaatcgaa gaagtatatg caagtattga agaacagctt gtaagtcaaa atagcgcact
     3901 taacgaaacc ttgtcagtgg ctttacaaga cttatcttcc agcgttccca atgttgcgtt
     3961 agaagagttt ggaggccaaa tagactctat caatatgcaa ttggacaata ttgtgcagac
     4021 attggtaccg agttcagaac tcagcaaccc cgcttttaat gccagcgtag tagctaggct
     4081 attgcatatc gcagggcacg aatatgaaga agctgttgca aatggcacaa taatagaaat
     4141 tgtagaatac caagatgggc aagcattcat ccacagagct cagagtatat tcaattcatc
     4201 agcaagtagt atcaaccaga gtatggccca ggaggtagag gaggtaaatg aactcttttc
     4261 taacttgaat aatgcattaa acaatagaga tgaccctgcg aatgtagaaa caacaataaa
     4321 tggaataata catgagctag cagaaatcac aggactctct gagagtcaac taactggcga
     4381 agaggctggc ggagaagagc aggatcctat tgcaataata aacaccatca aatctatgtt
     4441 gacccaactg atagctgcat atggatcaca agattatcaa ggagcagaga gcattgcaat
     4501 tgaggcatac ttggagaact atgaattcat agaggcaccc attgcccaac gtgatcaaca
     4561 attgatggaa caaacagaga taatgttaag agaagagctt aggcagatga taacagatag
     4621 agttccaata gaacaagtag aacaacatat tgctatgata aatgcgaatc tagatagagc
     4681 cacggagttg cttcaatagc agcaggaaat aataacaaga gataacccaa aatatttttc
     4741 cctccttctt tttcctatct taagattttc ttacttctaa catttctctt ttcgtttgag
     4801 agctgtgtta tgatcaataa gattattata gtattattga tatgtctttt tgcatttatt
     4861 tggacgcttc aatgaatcca acttgatcat ccgcaattca tattgcttta aggcatccat
     4921 agcaatctct agttttattg aaatgccatc tacttatact cgaataatgc ctctgcttgc
     4981 ctatacatag gcgctgctaa aggctcttgc cttcttccct actctcatct tgtcttacca
     5041 aatcttctct agatttgcgt gcactttcta tgagtctctg tcgtcttggc cgaatgatca
     5101 atatgtacaa tgagcctact acgtatacgg caagaagtgc gatttgggcg acgattgtct
     5161 caaccgttgg atggatgcca gtcatcgttg ccaagttgat gtcaagtctg ggcactgttc
     5221 cgataagatg tgttgtggat atatagccta cttcttgaaa ttcgcgtatt gcgttgccca
     5281 taaaagctat tgacatgtac gcgccaatcc ccattgtcag tgcaaataag acctttagag
     5341 ggagtcttct tccaagcttt ctcacgacaa ttgtggttgc aactattaca cccaagccta
     5401 gaacaagtcc tgcaacaacg taccattcca tatatttggc aaaggaaagc attgcttgat
     5461 aaaataatac agtctcaaaa ccttccctat atactgtaaa gaaggaaagc aatacaaaaa
     5521 ccattatgct gccagtagtt gttgctttcc agactttggc ctttacgaat tctatccaac
     5581 gctttgtttc gatcttgttc aatacccaaa agcttaccca aaatagaacc gcgactgcag
     5641 agacccctgc aatggcttct ataagctcac ggcttgctcc agaaatatct atgatgaatt
     5701 cagctataaa ccaagtaact gcagttgctc cgattgctat cactatgccg tagtacacat
     5761 gttttttgaa tctgttattt cttgatgcct ctaagtatgt caagatagca cctattatta
     5821 gtgcagattc tagaccttct cggaatatta tggaaaagga tgtggagaac gctatcgtcg
     5881 gcgctacaat gccagtacca gatactaatc tttctgattc atcaagccct cttctaattt
     5941 caacaatttt ggcttcgacc tgttcatacg gtgtatgagc ttgaattaga tttctaagtt
     6001 ctgcgaactt gatttccatg tcaagagtaa aatccggatc tataggacgc aagggaatct
     6061 ctatactctc atagctatcc aagtatgcag acctggatgt aagaagtgca ccatcataat
     6121 ttccttcctt atgtagccgg agtgtctcat caagctgcat cctgatcgta tcaatatcgc
     6181 tgcgaacagc ggctttggca ggatcttcgg cctcgccaat atccctacgc tcaccagtat
     6241 aggtaccaac tccggccttt agcccttcaa tgtctgcaaa aatgccggaa gaagaggttg
     6301 gcgctgatga actttggtta aatgatgcat catctttcaa cagctcctct gccttgtcaa
     6361 gttttatgag gatgccattg acaaaagctt ctatagactc tacggattct cttgcttcta
     6421 tcatcctgcg cagctcttct ctcatctcct cttcaattgt taccattaga tctggatcgt
     6481 gcttttcaat tggtgcttcc aaatattcat aattatccaa gtatgcagag atagcatatt
     6541 ggtctgcctg ctcataattg ccattgttta cttccgaaat aacctgtgac aaaagcgttc
     6601 ttattgtagc aaaatactgc tgatgttcac ttgtgctctc gcttccctcg cttactgata
     6661 gttcttctgc caagtctctt tcgattgcag tagtgaggcg aagaattgcc tcgttatcta
     6721 tcttttgagc tagagagctt tcaagttcat tgaagaacga ctcaatttca gttcttcttc
     6781 tattatcttc tatcaatgaa gacaaagttt tttgataatt ttggtgagct atctggacaa
     6841 ggccaactgc attttcgtaa tcaatagcct gttgtatggt agtactgtca gacccgtttg
     6901 atgcattaga aattgttgtt gcatatgact gtactgcatc ccgcaagaga aatacaacag
     6961 cttgggaaac catccctttg tcagaaagaa attgcgggcc tatagattgt tgagagattt
     7021 ggtccagcag tattcgtatt tgccctatac tttctctggt tttttcttct gaatactctc
     7081 cggttctcat tctcagggga aggtcggtaa gtaatgattc aagctgcttg gtggattgtt
     7141 ctccagcagt ctttcccagc tgatccttta tgctaggaaa tgtcgtggtg tgggggatga
     7201 aagcatgtgc aaatgccatt tcaggattgt ttttatccag actttgttca gtcaaccaaa
     7261 gctgcgttct cattctctca atgttgacca taacgataag ggcatctgtg tcgctaagca
     7321 cttggccata cacagagaag gaatttgggg caaatagttg agtaattgct attattatta
     7381 cgaaatagaa gattagtttt cttcttggct tgattgtggt ttgatctatg atattttttt
     7441 ggttcaattt attttcgatc accagtgtca atgtttctgg tatgcagagc ttacagggat
     7501 gttgttgttt ccaaaggcta tctgttgtgc tgtctttaga atagaagttg cggataatat
     7561 tttcatgtaa gaaacagtat ctgcaatgac atgcgaactt gggatcgcgt ctttgtattt
     7621 aaatgacaaa gtgctgccca ttaattaggt cgacctaatt agtttgacct aatatttaaa
     7681 ctgtctttcg tagggtattg taaacttgtc gtttgctgcc caaaattttc tcatgttaac
     7741 tggtatttgc tagttttcgt ctatcaatgg cgcttcttat ttttttctag ctcattctgt
     7801 atagggcgta attcgctgaa ttgatatgct acagtttcac tatcgttgcc aatgttgccg
     7861 aagtctatct gcaatggtga aatttcgaaa tcctcgccac gcgcgtcctt acgcttgatt
     7921 accatcgtca agatttcgcc gtttagcctg tccattttgt tattactcta gcctatgtct
     7981 aggtgaatat gcctaatatc ctatgcatgt tcactgcgaa tgctgaagta ttctttcttc
     8041 catgcttggt taaaattaag gatttatcac cgatcagcct ctccaccttc attcctgcaa
     8101 tccgctctcc atctttgaat gtggttgtat cgtttctcat aaaagatctt catgctacgc
     8161 tataatctgc tcttttaatg agctcaagtt agttacaaca ttttcccttt ggaatattat
     8221 ggccatgggg gcattttcta gggtgcttaa gaaggatgca aagggcatct gtaaagatgt
     8281 ttttcatatg atgttctatt ccgcaggcca tttcttcatc tacatcgatc tttagcgcgt
     8341 ccttcatcat aacttccagt agcctagtat ttctaatcat ctgccttcca attctctgtc
     8401 catcttgggt aagtgcaacc ttggtttggc tgtattccac aaggtttgaa ttgtgtagct
     8461 ttcgtaacat ttgtacaaca gaaggttgtg tgacattgag caactttgct attgagctaa
     8521 tcttagcatc ctgacctttt tcatgaagga accaaatggc tttaaggtac atctctatat
     8581 gttctgattc cgcagtacct atgaaaagct cttcttgatt ttgattgatg cggattctat
     8641 tattattatt tgacatatag tcagatatat tggcgctagc ctctcgtatt tttgccacaa
     8701 ttattcaaat aattaatagc tattatactt cttaaacaat atgctaccat aataatttat
     8761 tataacaaga tctataaaga tcacaataat acgcccaatt acttggattt tgttcattga
     8821 acccacttta gtgttatggt tggtgttatc ttcgccatca aactgatttc tttcagctag
     8881 gtagcgtgcc tacaggatct tacatcgttc ttgacagaaa agagtgcagc ttttagcacc
     8941 gtgtcctcgg agcattgggc taacttctat cctttatttc attc
//
LOCUS       DASQTF010000127         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1336152,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000127 DASQTF010000000
VERSION     DASQTF010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336152"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VE308_03000"
     CDS             complement(<1..303)
                     /locus_tag="VE308_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZA99971.1"
                     /translation="MSYRPVCKSDEIGEKSMKKFNVNHTEVLVGRLHGRLFACNNSCP
                     HRGASLVKGELKGDNVVCWMHGYEYDVFTGKLQNMKSWKKEDTWIEQSSEWRKSGDL"
     gene            complement(365..964)
                     /gene="dps"
                     /locus_tag="VE308_03005"
     CDS             complement(365..964)
                     /gene="dps"
                     /locus_tag="VE308_03005"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HZA99972.1"
                     /translation="MSNPQSPKGIVPNIVALEVLRKNGIDVDKLIKLVTAGVGAEFTT
                     YYYYTILRMHCTGLEGEGIKEIVEDARIEDRNHFEAMVPRLYELGGSLPRDIRKFADQ
                     AGCPDAYLPDNWEDINSILKVLLEAEQCAIRSWGEVCDMTAGKDHRTYDIAQRIMQEE
                     VEHEAWFIELLSKRPSGHFRRKFPGQSPHTGSQGSLVHL"
     gene            1466..1744
                     /locus_tag="VE308_03010"
     CDS             1466..1744
                     /locus_tag="VE308_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99973.1"
                     /translation="MKKRKPVLTIDDSSDSLKAMDLLKEKGIEFVEYDIRKFAESCCG
                     DLPTTKAPSVFAPEGVFKGLSGVEEYITAVLASVAKEAIPRESESAYW"
     gene            2100..>2704
                     /gene="ilvC"
                     /locus_tag="VE308_03015"
     CDS             2100..>2704
                     /gene="ilvC"
                     /locus_tag="VE308_03015"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZA99974.1"
                     /translation="MSAKKWLEDEVSLAPIKNQTIAVIGYGIQGRAQASNMMDSGLNV
                     IVGLRKGGKTWKQAEAESHKVMEVAEAAKSADIIHVLIPDMEQADTYTKEIAKHVTEG
                     KALSFSHGAAIHWKWIVPPKNVDVIMVAPKGPGQRVRELYQEGFGTPSLVAVFQDHTG
                     KAWDRVLAMAKAIGSTRPGVLQTTFKEEVETDWFGEQVDLCG"
BASE COUNT          791 a          573 c          592 g          748 t
ORIGIN      
        1 cagatcgcct gatttacgcc actcggagct ctgttctatc catgtatcct ccttcttcca
       61 tgacttcatg ttttgcaact tgccggtaaa aacatcatat tcatatccgt gcatccaaca
      121 tacaacatta tctcctttca attcgccttt aacaagcgac gctccacgat gtgggcatga
      181 attgttacag gcaaagagcc tgccgtgcag ccttccaaca agaacttcag tatggttcac
      241 gttgaacttt ttcattgact tttcccctat ttcgtcactc ttacaaactg gtctatatga
      301 cacaagagaa aaaaaagaaa aaaaggataa taaaaattga taagaagaag gagaagccct
      361 tatcttacag gtgaacaagg ctaccctgag agcctgtatg tggtgactgg ccagggaatt
      421 tccttctaaa gtgacccgaa ggtctctttg agagaagttc tatgaaccat gcttcatgtt
      481 caacctcttc ctgcattatt ctttgggcaa tatcgtacgt cctatggtct tttccagctg
      541 tcatatcaca gacttcgccc caggatcgga ttgcacactg ctctgcttca agaagcactt
      601 ttagtattga attgatgtct tcccagttgt caggtagata tgcatcaggg catcctgcct
      661 gatctgcgaa ttttctgatg tctcttggaa gggagcctcc aagctcatat aggcgaggaa
      721 ccatggcctc aaaatggttt ctgtcctcta ttctagcgtc ttcaacaatt tcctttatgc
      781 cctccccttc aagacctgta cagtgcattc ttaagattgt ataataatag tacgtcgtaa
      841 attctgctcc cacgcctgca gtaaccaatt ttattagctt atctacatca atgccgtttt
      901 ttctcaaaac ctctaatgca acgatatttg gtacaatgcc ttttggactt tgtggatttg
      961 acatacaacg tacctcaatt tataacataa aaggatttcg cactagtatt caaattataa
     1021 aaagtagtca tgcatatttt tcgaagtagg ccctttccac aggcagcgga tattgcggat
     1081 gtcggattaa tgaacgaccc ttgaaaatat tctgctcaca attagcctta gatgcgcaag
     1141 cgcagtccat ctccaaataa cgtattctac tgcagtttaa gatgtcgctg cctggagaga
     1201 aatatgaaat atagtaaaaa gcatacccct ttgcatgtta aaaattggac gggatagcag
     1261 agggtctggc cgcatttttt tgcagagagg ttttttgtat gatgactgga ataaagacac
     1321 tctgcaggtt atgatgccac agaatcaggt ttcacggaat gcaaattcaa caagagtagg
     1381 aagcttagat tatattacaa tatattcccg aggcttgaga attttatgtg ccaccatatc
     1441 gagtcgaagc ggaggtagac atggaatgaa aaagcgcaag ccagtactta ccattgatga
     1501 cagttcagac tctcttaagg ctatggatct tttaaaggaa aagggcatag aatttgtaga
     1561 atatgacatc agaaagtttg cagagagctg ctgcggcgac ttgcctacaa ctaaggcgcc
     1621 atctgttttt gcacctgaag gagtattcaa aggattgagt ggggtcgaag agtatatcac
     1681 tgcagtatta gcgtcggtgg ccaaagaggc gatcccacga gagtctgaga gtgcctattg
     1741 gtagctattg aaaaagaaca ctacctgcca ctacgaatac atcagccttg attttttcgc
     1801 cagcataagt gctatgtagg tagagtacat ttttgcagtg caagcaacag ggtttatcgt
     1861 atgacgcata aatgaggtca cttgcaacag taagcttatt ttttgctgca gtcatagtta
     1921 tttcgctaca ttcgacaaaa tatagctaca tggtattcag tcattaatgt tctgcctgct
     1981 atcctttcaa tgttattgat aggcattagt tggattgaga acaatataat taatttattg
     2041 aaagaagaaa tcaaggtcca tcagtagaat aatataatac atgccagcga cagagtctca
     2101 tgtccgccaa gaaatggctt gaagatgaag tatcgctagc tccaataaag aaccagacaa
     2161 ttgcagttat tggttatggc atccaggggc gcgctcaggc atctaacatg atggattcag
     2221 gactcaacgt aatagtcggc ctcaggaagg gaggtaagac atggaagcaa gctgaggcgg
     2281 aatctcacaa ggttatggaa gttgcagaag ctgccaagtc agctgatatc attcatgtcc
     2341 taatcccaga catggagcag gctgacactt acacaaaaga aatcgccaag cacgttacag
     2401 agggcaaggc gcttagcttt tctcacggag cagcaatcca ctggaagtgg atagtcccgc
     2461 ccaaaaacgt tgatgtaata atggtcgctc caaagggtcc ggggcagcgc gttcgtgagc
     2521 tatatcagga aggatttggc acaccatcac tagtcgctgt ttttcaagat catactggca
     2581 aagcatggga tcgggttctt gctatggcaa aggcaatagg cagcacacgc cccggggttc
     2641 tacaaaccac gttcaaagaa gaggtagaga ctgattggtt tggcgaacag gttgacctct
     2701 gcgg
//
LOCUS       DASQTF010000128         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_675262,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000128 DASQTF010000000
VERSION     DASQTF010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675262"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..2262)
                     /locus_tag="VE308_03020"
     CDS             complement(178..2262)
                     /locus_tag="VE308_03020"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZA99975.1"
                     /translation="MVEATLGFEGLAINAWLVWMLPFVGAAAIAGVARYGQKIRNYFA
                     VGLSLASAISATTLLPLGIAGGEIHSQVPWISVLGLEAGVLADPLSILMSNLVAWISF
                     LIFVYSTGYMHGDRDLTRFWFFMLFFIGSMQLIVLSDNLLMVFFGWEGVGFASYALIG
                     FWYHDRKKDYVGREGERAAGIPLWASPTHSGMKAFLMTRAGDTMMLAGMFLIFAYAGT
                     FGFRELIENQEWANQMAQQNLLVPAAVLLFGGAVGKSAQFPLNEWLLEAMTGPTSVSA
                     LIHAATMVKAGVFLVARIGPLFFALAAFGMTQFFEVVAWTGAITALLLATQAIVNPEI
                     KKVLAYSTGSQIGYMMMALGIAGLSTQFVEGYTAGFFHLMSHALFKASLFMGAGALLH
                     TVGSRFLTNMGGMRRHMKKTYIFMMLSGLSLAGAPLITSGFWSKDAIFASLLESGYTY
                     SWQLFAIAFAVAMMTAFYTFRMLGMAFFGKESRHLEEMQHEGHHLHEVGKSMWIPFGA
                     LAVATIFIGLIGFIFEDQIHHLLGEYLADSFGIAEEGQGGSESAVGEEEEAQESGAFL
                     GLNMTALIGSVGAFAVGGALGYLFYIARKVNPEVISRNAVTRSIWKFLYNRWYLNSAL
                     YWGSVIGPLAIYRIVWRYFENVVIDGMNPTLQFSMTYFSRVVRAAQTGITQTYLYIFA
                     AGIVIVTMLLFL"
     gene            complement(2304..>3135)
                     /locus_tag="VE308_03025"
     CDS             complement(2304..>3135)
                     /locus_tag="VE308_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZA99976.1"
                     /translation="KVLAYSSISQMGYILFGIGSESILGITGGALFYVTHGLGKAILF
                     MMAGSIILQTGTRSMSKLGGLAGNMPYTAVISMIGALTIIGIPPTSGFMSEWILFGGV
                     LQTALSQSDPTRVVLFGFGILTTVLSSAYILWMYKRIFFGKIPERLVNVKDSSAYITA
                     TMAVLAGLTLLLGVYPDPILNPITNYVESVFPENSGVLQLPSQEESALVKQPPEQSSS
                     GFQQSSIGRPNNNLLDQYSKPGILTPAKGNIAGEGQQKESVARFEHTALIYSKTGASK
                     "
BASE COUNT          919 a          775 c          624 g          817 t
ORIGIN      
        1 cctaaggaga ttataagcgc tccaaacgcg atcgcactat aaagtcggtt gttatgacct
       61 cgctccttct taagagcatc aatgacaggg attgcaagtc ctacaactcc caagacaatt
      121 gtgactataa taggagttga ccctagttcg actaccattt tagatccttc atttctccta
      181 tagaaacagc agcatagtga ctatcactat acctgccgcg aaaatgtaaa gatatgtttg
      241 ggttatccct gtttgtgctg ctctgacaac tctagaaaaa tacgtcatgg agaattgtaa
      301 agtgggattc atgccatcga taactacgtt ctcaaagtat cgccagacaa ttctgtatat
      361 cgcaagaggt ccaattaccg atccccagta aagcgcggag ttaagatacc atctgttgta
      421 aagaaacttc caaattgatc ttgtgactgc atttctcgaa ataacctcgg ggtttacttt
      481 cctagcaatg taaaatagat atccaagcgc acctcctact gcaaaggccc ccactgatcc
      541 tattagggcg gtcatgttga gccccagaaa agctccgctc tcttgtgctt cttcttcttc
      601 tcctactgcc gactctgaac ctccctgtcc ttcttcggcg atgccaaacg aatcagcaag
      661 gtactctcct agaaggtggt gtatttgatc ttcaaagata aagccaatca agccgatgaa
      721 tatagttgcg actgccagag ctccgaacgg aatccacata ctttttccaa cttcatgtaa
      781 atggtgacct tcatgttgca tctcttctaa atgtctgctc tcttttccaa aaaatgccat
      841 gcctaacatc ctaaatgtgt aaaaggcagt catcatagca acggcaaaag caatcgcgaa
      901 caattgccag ctataggtat agccagactc aagcagcgac gcgaaaattg catccttgct
      961 ccaaaatccc gatgttatca gcggagcacc tgcaagtgaa agacctgaaa gcatcataaa
     1021 aatatatgtc tttttcatat gcctacgcat tccacccata tttgtcaaaa accttgagcc
     1081 aacggtgtgc agcaatgcgc cggcacccat gaaaagtgat gccttaaaca aagcgtgact
     1141 cattaagtga aagaatcctg cggtatatcc ctcaacgaac tgggttgata atccagcaat
     1201 accaagtgcc atcatcatat aaccaatttg tgatccggta gaatacgcca ataccttctt
     1261 aatttcggga ttgacaattg cttgggtagc aagaagaaga gcagtgattg cacctgtcca
     1321 ggcaactact tcaaagaact gagtcattcc aaatgctgcg agagcaaaaa acagaggtcc
     1381 aattcttgca accaaaaaca cacctgcttt gaccattgta gcggcgtgaa ttaatgcgga
     1441 aaccgaagta gggccggtca tagcctctaa tagccattcg tttagtggaa actgtgcaga
     1501 cttgcctacg gccccaccaa acaatagtac cgcagccggg actaacagat tttgctgtgc
     1561 catttggttt gcccattctt ggttctcgat aagttctcta aacccaaatg ttccagcata
     1621 agcaaatatg agaaacattc ctgcaagcat catagtgtct cctgctctgg tcatcaaaaa
     1681 cgccttcatc ccagaatgcg tcggagatgc ccataacggt attccggctg ccctttcacc
     1741 ttctcttcct acataatctt tcttgcggtc gtgataccag aagccgatca atgcatatga
     1801 cgcaaagcca actccttccc atccaaagaa aaccatgagc aagttatctg aaagtacaat
     1861 cagctgcatc gatccaatga aaaagagcat gaaaaaccag aatctagtca ggtctcgatc
     1921 tccatgcata tagccagtag aatacacaaa aattagaaag gaaatccatg caaccagatt
     1981 agacatcagt atcgacagcg ggtcagccaa tactcccgcc tctaagccta gtacagagat
     2041 ccaaggtact tgactatgga tctctccccc agcgatgccg agaggtagca aggttgtggc
     2101 ggatatagcg ctggctagtg ataatccgac tgcgaaatag tttctgattt tttgcccgta
     2161 acgtgcaact ccagcaatgg cggcggcacc aacgaagggc aacatccaaa cgagccaagc
     2221 gttaattgca agtccttcaa aacccaaagt agcctctacc atttggtcta gccagccgcc
     2281 tctatagtct tattatccaa tatttatttc gatgcccctg ttttgctgta aattagtgct
     2341 gtatgttcaa acctagccac agactccttc tgttgacctt ctcctgctat atttcccttg
     2401 gcaggagtaa ggataccagg tttcgagtat tgatcaagga gattgttgtt cggccttccg
     2461 atgctagact gttgaaagcc ggaactggac tgttcgggtg gctgcttaac aagagcactc
     2521 tcttcttgtg atgggagctg caacactcca gaattttcgg gaaatactga ctcgacataa
     2581 tttgttatag gattcaaaat agggtctgga taaaccccta gaagcagagt caatcctgct
     2641 aaaacagcca tagtagcggt gatatatgca ctagaatcct taacgtttac aagtctttca
     2701 gggatctttc caaagaagat tcttttgtac atccatagga tatatgcaga agagagtaca
     2761 gttgttagaa taccaaaacc aaacaatact accctcgttg ggtcagattg ggatagcgct
     2821 gtctgcaata ccccgccaaa caatatccac tctgacataa atccactggt agggggaatc
     2881 ccgataattg tcagcgcccc tatcattgag ataactgcag tatacggcat gtttcctgca
     2941 agccctccta gttttgacat gcttctcgtt ccagtttgaa ggattattga gccagccatc
     3001 atgaaaagaa ttgccttccc aagaccgtgt gtaacataga acagcgctcc ccctgtgatc
     3061 cccaagatgc tttcagagcc tattccgaac agtatatagc ccatttgact tatactggaa
     3121 taggccagca ctttt
//
LOCUS       DASQTF010000129         4674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_305895,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000129 DASQTF010000000
VERSION     DASQTF010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305895"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..964)
                     /gene="folP"
                     /locus_tag="VE308_03030"
     CDS             complement(128..964)
                     /gene="folP"
                     /locus_tag="VE308_03030"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZA99977.1"
                     /translation="MPVGKKKFTVKVIGIINTSPESFYKGSVKTGEQEIAATARQMQE
                     DGAHMIDVGGMSTAPYVQTALVPIEEEIRRVTKAIKVVRSACDLQISADTPRADVAKE
                     AIAAGADAVNDVTGLKYDSKMADVVAKAGVSMIIGAYSKAPATGKMVSTLKTLKKSIR
                     LASEAGIKDVIIDPSIGFFRQEGKNPFFTKMTDMPWYARDIEILSNLHKLATLKKPIC
                     VSVSRKSFIGHLLSLKAAEDRLIPSIACEMIAALNGANIIRTHNVRETVQAVTMLQLL
                     RT"
     gene            1079..1660
                     /locus_tag="VE308_03035"
     CDS             1079..1660
                     /locus_tag="VE308_03035"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HZA99978.1"
                     /translation="MKQQDLDTAKAMHNVPTRVFFTKGRGIHKDYLTSFELALRDAGI
                     ADLNLVSVSSILPPHCKIVSCQEGRKYLQPGQIVFTIMARSATNEPNRLIAASIGLAR
                     PADGSQHGYLSEHHSTGETAQKAGDYAEDMAMEMLATILGLPNDPSLAWNEKEEQWRL
                     SNKIYRTQNFTQSAEGHKDGLWTTVISGAVLIL"
     gene            complement(1650..2180)
                     /locus_tag="VE308_03040"
     CDS             complement(1650..2180)
                     /locus_tag="VE308_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99979.1"
                     /translation="MGVITWIAIAVIVLVVIGLGAGVFFSGLIRGAEIIRNNPAVQNV
                     TQEAKEFLNNNIQGSSGSSSALVITTNEARYRIGEPVTITVRNIGDETLTFPNSAVGL
                     KIQNTNTGQIYNIAAAQVTTELEPGASKAMTWNQNDGNGNNVPAGDYTATVQTISSSS
                     SSAQSVSAQVSFEIIE"
     gene            complement(2426..2761)
                     /locus_tag="VE308_03045"
     CDS             complement(2426..2761)
                     /locus_tag="VE308_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99980.1"
                     /translation="MQPSDIEDILPPEKIAFIAYNVGIFESVQKFGNLITSSKITGGM
                     DPVNVAELLEETRAFYDAEMIAQLINGMLEQRTSMDLPSHLTIGRVTAGQVDYVIRQL
                     KAAGISINK"
     gene            complement(2943..3272)
                     /locus_tag="VE308_03050"
     CDS             complement(2943..3272)
                     /locus_tag="VE308_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HZA99981.1"
                     /translation="MRVWDIEAELLCNSHLLGEHREIHAIWTILTENKKGYSHHPETL
                     RWKGKLLALYNIHETVVAEMNKRGFNHKSTLKKELATGSNMQSLFVDQPARQRMILLN
                     KRCKCKL"
     gene            complement(3412..3750)
                     /locus_tag="VE308_03055"
     CDS             complement(3412..3750)
                     /locus_tag="VE308_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99982.1"
                     /translation="MSYVSDDRNYTDPNNSGIDSISRVGEITGEPPTNKAVDRDKAAK
                     MANLLEDLKFPATKEDIKDHINRKSPAMGNRINDIFEVVWNNLGDNVTYNSAYEVEKA
                     AGLVEKADRK"
     gene            complement(3805..4146)
                     /locus_tag="VE308_03060"
     CDS             complement(3805..4146)
                     /locus_tag="VE308_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZA99983.1"
                     /translation="MAARSVGHFEIPADRTDELKNFYSSLFGWQYEKGQTGDYWMIKN
                     AGISGGIAPRENPEQMPTMFVTVESIEDYISKAQQLGAKVVKNKQEIDAGYYAVMEDT
                     QKNTFGIWQDK"
     gene            complement(4166..4630)
                     /locus_tag="VE308_03065"
     CDS             complement(4166..4630)
                     /locus_tag="VE308_03065"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZA99984.1"
                     /translation="MTRVEHEVDINAPVERVYEYYTNPDNIKEAWPRDIVKESESTTS
                     SKNEPGSEMKVKGEYMGRQAEMRLEVAEKQPNRKLVTRQTEGPFKRWESIQEFQGDNN
                     RTHIRHVVDYELGSLEKAGNFVTGSQADDKIRQGLNQAAQTVKQKLESQQSR"
BASE COUNT         1124 a         1085 c          940 g         1525 t
ORIGIN      
        1 gatctggaaa ttatcggtga ccgtttagga acgagaagta cgtcgtcaaa tgtcagaccc
       61 tgtatgatgt ccaactattt ccttgacgtg taacagctct gaagcattat aagcgtttgc
      121 caccaaccta tgttctcaac aattgcagca ttgttactgc ctgcaccgtt tcgcgaacgt
      181 tatgtgttct gatgatattt gcaccattga gagcagcgat catttcacat gctatggagg
      241 gtatcaggcg atcttccgcc gccttcaggc tcagcaggtg cccaatgaaa gactttctcg
      301 aaactgatac acagatcggc ttcttgagcg tggcaagctt gtgcaggttg gataggattt
      361 caatatcgcg tgcataccat ggcatgtctg tcattttagt gaaaaaggga tttttccctt
      421 cttgacggaa gaagcctatc gatgggtcaa taattacatc tttgataccc gcctcacttg
      481 ccaatcttat gctcttcttc aatgtcttga gcgtactgac cattttgccc gtagcaggcg
      541 ctttgctgta tgcgccaatt atcatagaca caccggcttt tgctacaaca tctgccatct
      601 tgctatcgta ttttagtcca gttacatcgt tgactgcgtc tgcgccggct gctatcgctt
      661 cttttgcaac gtcggccctt ggtgtgtctg ccgaaatctg caagtcgcag gcactcctga
      721 cgaccttaat ggcctttgtg actcttctta tttcctcttc gattgggacg agggcagttt
      781 gcacataagg cgcagtcgac atacctccaa catcaatcat atgagcgcca tcctcttgca
      841 tctgccttgc tgttgctgct atttcctgct cgccagtctt gaccgagcct ttgtagaagg
      901 attcagggct ggtatttatt atgcctatta cttttaccgt gaacttcttc ttaccaactg
      961 gcattgctct gatcttcaac gctccgatct agtcgccaca tctatgctat atagttgcaa
     1021 caatattact gataatataa tatcaattct aatttgactt gtattccaga cattgtgcat
     1081 gaaacaacaa gatctcgata ctgctaaggc tatgcacaat gtccccacta gggtattttt
     1141 caccaagggc agaggcattc acaaggacta tttgacaagt tttgagcttg ccctccgaga
     1201 tgccgggata gcagacctca atctggtgtc agtatcaagc atactccctc cgcattgtaa
     1261 aattgtcagc tgccaggaag ggagaaaata cttgcagcct ggacagattg tatttacaat
     1321 aatggcaagg tctgccacta atgagcccaa caggcttatt gcagcatcaa ttggtctggc
     1381 aaggcctgcg gatgggagcc agcacggata cctgtcagag catcactcta ccggcgagac
     1441 tgcgcaaaag gcaggtgact atgccgagga tatggcgatg gaaatgcttg caactatact
     1501 tgggctgcca aacgatccaa gccttgcctg gaatgaaaaa gaggagcagt ggaggctatc
     1561 aaacaagatc taccggacac agaactttac ccagtcagca gagggccata aagatggcct
     1621 atggactaca gtaatcagtg gagccgtact tattctataa tttcgaagct aacctgtgct
     1681 gagacactct gagccgatga tgatgatgat gatatggtct gcacagttgc agtataatcg
     1741 ccagccggaa cattgttgcc gttgccgtca ttttgattcc aagtcatcgc ttttgatgcc
     1801 cccggttcca gttcagttgt tacttgggcc gcagcaatat tgtatatctg gccggtgttc
     1861 gtgttctgaa tctttagacc tactgccgag ttgggaaagg tcagcgtctc atcgcctatg
     1921 ttcctgactg tgattgtaac cggctcaccg attctgtacc ttgcctcatt agttgttatc
     1981 accaaggcac tggagctgcc actgctcccc tgaatgttat tgttgagaaa ttctttagcc
     2041 tcttgggtaa cattttgcac tgctgggtta ttccgaatta tttcggcacc ccttatcaat
     2101 cctgagaaga agacgcccgc cccaagcccg attactacga ggactattac cgcaattgct
     2161 atccaagtta tgacgcccat aatctattgc aattcagcaa gtatcttaat gattttgaat
     2221 ttctactcta gcgaaaatcg agtaaagaat tatcagtcag acaattatta atattattag
     2281 taagtgctta gtctatttca gaaatagctc agaattacaa taactgagaa tatacagcaa
     2341 acaagcatgc aagcaaggaa ttgattaagc cctatggttg ttgtttatgt tgatatcgac
     2401 gatgttattg tggcggtggt gcgatttatt tattaatgga tatgcctgct gcctttagct
     2461 gcctgatgac atagtctact tggcctgctg tgactctccc aattgtcagg tgggaaggaa
     2521 gatccatgct cgtcctctgc tccagcatcc cattgataag ttgtgcgatc atctctgcgt
     2581 catagaatgc acgagtttct tcaaggagct ccgctacatt gactggatcc atcccaccag
     2641 ttatcttgct actggtaatg aggttgccaa acttctgaac cgattcgaaa atcccgacgt
     2701 tgtaagcgat gaatgctatt ttctccggtg gtaggatgtc ctcaatgtct gatggttgca
     2761 tctattaata atacaaggct ttttcttgat aataaatcat aacttcataa caagatattt
     2821 ttactgtgtg tagttcaaat ttttgtgcga aggtttatgc ctaaattgag cataattatg
     2881 catattctgt ctgtagagct ggtttgctcg tctatgacat cttaaaaaga gctcagagtt
     2941 tcttataact tgcatttaca tctcttattc agcaagatca ttcgctgcct agctggttga
     3001 tcaacgaaaa gagattgcat gttggagcca gtcgccagct cctttttcag agtgcttttg
     3061 tggttaaaac cgcgtttgtt catttctgct actaccgttt catgtatgtt ataaagtgcc
     3121 aatagcttgc ctttccaacg caaagtttca ggatgatgtg aatatccttt tttattttcc
     3181 gtcaaaatcg tccagattgc atggatttcc ctatgctctc ctaataaatg gctgttacat
     3241 agtaactccg cctcaatatc ccatacacgc attactatga cactacatat tttgtttaat
     3301 caaatagata actatatgga ttctttttga tctgtagtga ttcaaatcag gacgcttgta
     3361 atttctgctg ctacagtttt cgatccatat ctctcgcagt atatcttcta attatttcct
     3421 atcggccttt tctaccaagc ctgcggcctt ctcaacctca taagcactat tgtaagtcac
     3481 gttgtctcct agattattcc agactacctc gaaaatatcg tttattcggt ttcccatagc
     3541 tggtgatttt ctattgatat gatccttgat atcctctttc gtggctggaa attttagatc
     3601 ttcgagcagg ttggccattt ttgctgcctt atctctatca acagctttgt tggttggagg
     3661 ctctcccgtg atctcgccta ctctgcttat actgtctata cctgaattgt tagggtcagt
     3721 ataatttcta tcatccgata cataggacat tgcggctctg tcactcctta gaaaacaaaa
     3781 gggtggtgct atggcaggga acgtctattt gtcttgccat atcccaaacg tatttttctg
     3841 cgtgtcttcc attactgcat aatatcctgc gtcgatctct tgtttgttct tcactacttt
     3901 tgccccaagc tgttgggctt tgctgatgta atcctctata gactcaactg tcacaaacat
     3961 agttggcatc tgctcaggat tttctcttgg agctattcct ccactaattc ctgcattttt
     4021 tatcatccaa tagtcccctg tctgaccctt ctcatactgc cagccaaata gtgatgaata
     4081 aaagttcttt agttcgtctg tcctatcagc aggaatttca aaatgtccta cgctgcgtgc
     4141 tgccattgac tatcccttcc cttttttacc tagactgttg tgactctagc ttctgtttga
     4201 ctgtctgggc tgcctgattt aatccctgcc ttattttgtc atcagcttga cttccagtga
     4261 caaagtttcc agccttctca agtgatccaa gttcatagtc tactacatgt cgtatatgcg
     4321 tacgattatt atcaccttga aattcttgta tactttccca cctcttaaat ggtccttctg
     4381 tttgtctagt tactaacttt ctgttgggct gcttttctgc tacctcaagg cgcatttctg
     4441 cctggcggcc catatattca cctttgactt tcatctcaga acctggctca tttttagaag
     4501 atgtagttga ttctgattct ttgacgatat ctctaggcca tgcttctttg atgttgtctg
     4561 ggtttgtata gtattcataa actctttcta cgggagcgtt tatgtccact tcatgttcta
     4621 ctcgtgtcat gataagggcg gcacaggatc ttatttatcc cattttcaac ttct
//
LOCUS       DASQTF010000130         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_861512,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000130 DASQTF010000000
VERSION     DASQTF010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861512"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /gene="tfb"
                     /locus_tag="VE308_03070"
     CDS             <1..745
                     /gene="tfb"
                     /locus_tag="VE308_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019310.1"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HZA99985.1"
                     /translation="ESKSRTGMPSSLAFHDMGLSTFISYSNVDANGVAISAEQRSKVQ
                     RMRRWNKISSNNRSYHRNLKNAFAILSTIKDKLSLSDTLIEKAAYNYRKALDKRIIKG
                     RSIRALIVAAVYASCRDLSVPRTLEEISSAANTDPIFAGKCYRLLVQNLELQLPVIDS
                     NKYLAKIANKAKVSEKTYRRALEMLSTIKNDPISHGKDPNALAVAVLYAACIREGENV
                     SQAQIAVSGDISIVTLRKRFQDVRKTFSS"
     gene            1068..2165
                     /locus_tag="VE308_03075"
     CDS             1068..2165
                     /locus_tag="VE308_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZA99986.1"
                     /translation="MVTVDQVLGSLKKVVDPELHKDIVSMGMIKDLSINDGKVAFTLE
                     LTTPACPFNSEIEQDVRNAIASLGVNDLNMRVTAKVMEGRSLSMDELLPGVKNIIAVA
                     SGKGGVGKTTVSVNLALALANAGAKVGLLDADIYGPSVPLMLGLKASPEVVNNKIQPP
                     EIEGVKVISMGFFYEQSQQAGIYRGPIVSGIVKQFLTDVSWGELDYLIIDLPPGTGDA
                     PLTIAQTIPITGILIVTTPQDVAMNVAVKAIGMFNKLNVPIVGVIENMSYLLCPHCNE
                     QIYLFGKGGGKKISEDFGLPYIGEVPLHPGIRESSDLGKPTVISEPESVQAHAFTKAA
                     KLIAGRISVLAAEIQAHEQSESQATEQANKE"
     gene            2165..>2541
                     /locus_tag="VE308_03080"
     CDS             2165..>2541
                     /locus_tag="VE308_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF359 domain-containing protein"
                     /protein_id="HZA99987.1"
                     /translation="MPTDTSDTHLLKRPFGTLVLEKDITKEKIVSMLEGAKKIISVGD
                     ATTERLISFGITPDIAVIDGMERRSLRNRTINYDAKEMICTNPAGTISKEAVHVLQCA
                     LETPYPVTVKVEGEEDMLALPLF"
BASE COUNT          784 a          516 c          588 g          653 t
ORIGIN      
        1 ggaaagcaag agtaggacag gtatgccgtc ttcacttgct tttcatgata tgggattatc
       61 tactttcatc tcctactcca acgtagacgc aaatggagtc gccataagtg ctgagcagag
      121 aagcaaagtt cagaggatgc gtagatggaa caagatctcg agtaataacc gtagttacca
      181 ccgtaacttg aaaaacgcat tcgctatatt gagcacaata aaggataagc tttccctcag
      241 cgacactttg attgaaaaag ctgcatataa ctacaggaag gctcttgaca agaggataat
      301 caaaggacga tcaattaggg ctctcatagt cgcggcagtt tatgcttcat gcagagatct
      361 ttcggtacca agaacgcttg aagaaatctc ctcagcagcc aacactgatc cgatatttgc
      421 tggcaagtgc tatagactgc ttgtgcagaa ccttgagctt caattgccag tgattgactc
      481 aaataaatac ctagcaaaga tagccaataa ggcaaaggtg agtgagaaga cctacaggag
      541 ggcacttgaa atgctatcga ccatcaagaa tgatcccatt tcgcatggta aggatcctaa
      601 cgcgcttgca gtagcagtac tatatgctgc ctgtataaga gaaggagaga atgtaagcca
      661 ggcccaaata gcagtttccg gcgatattag tatcgtcaca ctgcgaaaaa ggtttcaaga
      721 cgtaaggaaa accttctctt cctaattttt gataaatagc gagatttttc tctttcttga
      781 ttggtgtggt aacaactgaa gataatgcaa accttgatta tgtaaatagg ctcctacact
      841 tgtatatata gtgaatttat tttgcggtct ttttaaataa accgtcaaaa atacatacct
      901 ctcgcgatgg caacttgttg tttcgtattc agagataata aaaaacaatc actttatagc
      961 ctttcaataa aagagttggc taatacaatt ttaaagggtt tttttaagaa taggtgattt
     1021 ttcctccgat acgatgattt aaataccgta aggacaaatt ttttccaatg gtaaccgtcg
     1081 atcaggttct cggctcactt aagaaagttg tagatcctga gcttcacaag gatattgtat
     1141 caatgggtat gatcaaggac ctatcgataa acgatgggaa ggtcgcattt acactagagt
     1201 taacaacacc tgcctgcccg tttaatagcg aaatagagca ggatgtgcgg aatgcaatcg
     1261 catctctagg agtaaatgat ctcaatatga gggtcactgc taaagtcatg gagggcaggt
     1321 cgctttcaat ggatgagcta ttgccaggtg tcaaaaatat tattgcagtt gcaagtggca
     1381 agggcggggt gggaaagacc actgtttccg ttaatcttgc gcttgcatta gccaatgctg
     1441 gtgcaaaggt tggattactt gatgctgaca tttatggtcc aagtgttcct ctaatgcttg
     1501 ggctcaaggc atcgccagaa gtagtcaata ataagatcca gcctccagaa atcgaaggtg
     1561 taaaggtcat ttcaatgggt tttttctatg agcagtcgca gcaggccggc atttacaggg
     1621 gcccgattgt ttctggtatc gtcaagcaat tcttgacgga cgttagttgg ggtgagctag
     1681 attatttgat aatcgatctg ccgcccggca caggcgacgc gcctttgaca attgcacaga
     1741 cgatacctat tacaggtatt ctcatagtca ccacgccaca agacgttgca atgaacgttg
     1801 cagtgaaggc gataggaatg ttcaacaagc tcaatgtgcc gatcgtcggt gtcattgaaa
     1861 acatgagcta ccttctatgc ccacattgca atgagcagat ctatcttttt ggaaaaggtg
     1921 gcggcaagaa gataagcgaa gactttggat taccatacat aggcgaggtt ccactccatc
     1981 cgggtataag ggaaagcagc gatttaggca agccgactgt aatctcagag ccagagtcag
     2041 tgcaggcaca cgcctttaca aaggctgcca aactgattgc aggaaggatt agtgtcctag
     2101 cagcagaaat tcaagcacac gagcaatccg aaagtcaagc aactgaacag gccaataaag
     2161 agtgatgccg acggacacaa gtgatacgca tcttttaaaa cgaccgttcg gaacgcttgt
     2221 gcttgaaaaa gacattacca aggagaaaat cgtatcaatg ctagaaggtg caaagaaaat
     2281 aatctcagta ggcgacgcaa ctaccgaacg tctaatttct tttggcataa cgcctgatat
     2341 tgcagtcatt gacggcatgg aacggaggtc gctgcgtaac cgcaccatca actatgatgc
     2401 caaagaaatg atatgcacta accctgcagg cacaatttca aaggaagcgg tacatgtttt
     2461 gcaatgcgcg ttagaaacgc cttaccctgt tacagtcaaa gtagaaggcg aagaggatat
     2521 gcttgctcta ccactattta g
//
LOCUS       DASQTF010000131         4253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_306107,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000131 DASQTF010000000
VERSION     DASQTF010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306107"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VE308_03085"
     CDS             <1..297
                     /locus_tag="VE308_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99988.1"
                     /translation="TRLDIIFTVSPLITDLMLKGYVETIRRRRLFFFSREYCMITVEG
                     LAALEQARSLFENIVELIRERALETIDNIAADSPLLKLTVISAKAAYKTVKVLI"
     gene            564..773
                     /locus_tag="VE308_03090"
     CDS             564..773
                     /locus_tag="VE308_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99989.1"
                     /translation="MSAKESAVAKSRGSQDERDMSPPDAALLQVLYNKKKLQKLKLIM
                     RKMKNHVINHVLNASLTLVMKASAM"
     gene            1157..2200
                     /locus_tag="VE308_03095"
     CDS             1157..2200
                     /locus_tag="VE308_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZA99990.1"
                     /translation="MIESTFEAQKSISADNAIVVKGLRKSFKKLTVLEGIDLCVKRGT
                     VLALLGPNGAGKTTTIRILSTLLLPDGGQALIHGFDVVREADKVRSLIGLTGQYAAVD
                     EYLTGEENLHMIGRLYRLSHQNIKQRTQQLLELFDLMEASARTVKTYSGGMRRRLDLA
                     VSLIAAPPIIFLDEPTTGLDPRSRLIMWDIIEQLVAAGTTILLTTQDMDEADRLADKI
                     VVIDVGRIIAEGTSDELKQRVGSERLEITIAEASSFETAKRVIDGGDESLHLDIKART
                     LSLAATKGDGVHQLKQVLSQLDEAGIGVENVSFRRPTLDDVFLTLTGHTTTTIEQSNG
                     QRETEKSGGGLNK"
     gene            2200..3030
                     /locus_tag="VE308_03100"
     CDS             2200..3030
                     /locus_tag="VE308_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA99991.1"
                     /translation="MTRSSSSPFITANNVQTEKHSKVFWALSDSWVLAKRSIKHITRN
                     LDQLFAVALFPIMFFLLFHFVFGGAIDTGDLSYTNYLVAGILVQMLAFGANYTTLNLA
                     IDLQRGIVDRFRSLPMASSALLVGHVGADLFRNTISGLIVLGVSFLLGFQPVAGPVEW
                     LLVLALAILFSLAISWLSAIMGLLVKSIEAAQWTGFIIIFPLTFVSSAFVPTDTMPEA
                     LRFFAENQPLTHVIEAIRGWLVGAPIGDSGWLGFAWCVGIIIVAMPVATWLFRRQALR
                     "
     gene            3482..>4253
                     /locus_tag="VE308_03105"
     CDS             3482..>4253
                     /locus_tag="VE308_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZA99992.1"
                     /translation="MTGIEKQSSINRSAWITLVILSCLGLITMYGETMVLPAIPDFIR
                     DFNISYNTSSWILSSYLIAGAVMTPIAGRLSDIYGKKKILLIVMAVYSTGILAGGFAN
                     SIEFMLAARTAQGVGMSMFPIAFGIIREILPEKKLAIGQTIFSSTFSGGAVVGLIVGA
                     TIIQNFGWQATFFSIFPIAVILGLIIRKLINVRNLATQEVKVEGGGEVRNDENSSVKK
                     QTIDLKGTVALAVTIISFLAGISFLESNSNDAGFQVAGL"
BASE COUNT         1274 a          823 c          990 g         1166 t
ORIGIN      
        1 acccggcttg atattatttt tacagtttcc ccattgataa ccgatctgat gttgaaaggc
       61 tatgtcgaga caattagaag gcgtaggcta tttttctttt caagggaata ctgcatgatc
      121 acagtagagg gacttgctgc cctggaacaa gcaagaagtc tatttgaaaa catagttgag
      181 ctcattagag agcgggctct tgaaactatc gacaatattg cggcagactc acccctctta
      241 aaacttacag taatctctgc caaggcagct tacaagactg taaaagtgct aatataagat
      301 taataatata aaattactat ctcccatctg tgctagccat cgcagtatat ggaaaaagat
      361 tcttctccat ctctaaaaaa gcatattcat gcaatgattg tggacaagca agaaagcctt
      421 tgtagctatc gaagactcaa ctggagtctg caaataagga aggaatctta aagctttgct
      481 gttgtcagag tcggcctcag cacaacaacg ataatagtag cccaccacac accagtaaat
      541 agtatcaggg tttatcgtta accatgagcg caaaagaatc ggcagtagca aagtctagag
      601 gttcacaaga tgaacgtgat atgtctcctc cagatgcagc tttacttcaa gtgctttaca
      661 ataagaaaaa attacaaaag ttgaagctaa ttatgcggaa aatgaagaat cacgtgatca
      721 atcatgtatt aaatgcaagt ttaaccttgg tgatgaaggc aagtgccatg tagtcgaagg
      781 agaaatcaat aacgaaaaag gtagctcgaa gtttttctca ccaaaaggcg acggaatgtt
      841 gccgggagac attgtttgga tgtatgtaaa agaatcagga aagaaactga aatatgagga
      901 aggatatgtt atcgaagaag gcgctccagg atttcaatgc aaggattgca aatactatat
      961 atgtactctg gtagttgtct attcatagaa ggaaagttca agccagagat gagctgtggc
     1021 tttattgtga aagtaggaca tgggactgag atttagaaat cgacttttct ctaagcgctt
     1081 atctagcagg tttctcctcc tccctccctt taaagtgttc tagactttaa aaataattgt
     1141 ttggttatta tctttcgtga tagagtctac attcgaagct caaaagtcaa tatcagcaga
     1201 taatgctatt gtcgtaaagg gattgaggaa atcattcaaa aagcttacag tacttgaggg
     1261 tattgatctt tgtgtcaagc gaggcactgt tctggcgcta cttgggccaa atggtgctgg
     1321 aaaaactact actattagaa tacttagtac attgctttta ccggatggcg gtcaagcact
     1381 aattcatggc tttgacgtag taagagaagc cgacaaggtg cgtagtttga tcggtttgac
     1441 aggccagtac gcggctgtgg atgaatattt gacaggcgaa gaaaacctac atatgatagg
     1501 ccggctctac cgcctgagcc atcaaaatat caaacagaga acgcaacagc tacttgagtt
     1561 gtttgatttg atggaagctt ctgcccgtac ggtaaagacc tattctggcg gcatgcgccg
     1621 ccgactagac cttgcagtca gcttgattgc agcccctccg attattttcc tagatgagcc
     1681 cactacaggt cttgatcccc ggagccgcct aattatgtgg gatattattg aacagctagt
     1741 cgcagctggt acaacaatac ttcttactac tcaagacatg gatgaagcag atcgtttagc
     1801 tgataagatt gttgttattg acgtaggtag aattattgcg gagggcacat ctgacgaact
     1861 gaaacaacga gtaggttcgg agcgcttgga gataactatt gcagaagcaa gcagctttga
     1921 gacagctaaa agagtaattg atggtggtga tgagagtctt catttagata tcaaagcgag
     1981 gactctcagc ttagcagcaa ccaaaggtga tggtgtacat caacttaaac aagttctgag
     2041 tcagctagat gaagctggta ttggagttga aaatgtatct ttccgtcgtc ctacacttga
     2101 tgacgtgttt ctcacactta ctgggcatac aacaacaaca atagagcaat caaacggaca
     2161 gagagagaca gaaaaaagtg gtggaggtct aaataagtaa tgactagatc ttcttcttct
     2221 ccattcatta ctgccaacaa tgttcagacg gaaaagcact caaaggtctt ttgggcactc
     2281 agcgattcgt gggtattggc taagcgtagc atcaagcata ttactagaaa tctcgaccaa
     2341 ctcttcgctg tcgcactctt cccaatcatg tttttccttc tatttcactt tgtttttggc
     2401 ggtgcgatag atacaggcga cttgagttac acaaactatc ttgtagccgg catcctggtg
     2461 caaatgctgg cctttggcgc aaactataca acactgaatt tagcaattga cctacaacga
     2521 ggtattgttg atcggtttcg ctcactaccc atggcaagtt ccgctctcct agttggtcat
     2581 gttggagcag atctattccg aaatacaatc tcaggtttaa ttgtccttgg tgtcagcttt
     2641 cttctaggct ttcaaccagt tgcaggccct gtggagtggt tgctggtgct ggctcttgca
     2701 atcctctttt cacttgccat ctcttggcta tcagctatca tgggtctgct agtcaagagt
     2761 attgaggcag cacaatggac aggtttcatc atcatctttc cgttaacatt tgtcagcagt
     2821 gcatttgtgc caactgacac tatgccggag gcgctgcggt tttttgctga aaaccagcct
     2881 cttacgcatg taattgaggc aatccgtggc tggcttgtcg gtgcaccgat aggcgattcg
     2941 ggctggctag gatttgcatg gtgtgtaggc attatcattg ttgctatgcc agttgcgaca
     3001 tggctctttc ggcgtcaggc tttgcgataa atatggtata gaagcaacga aagagtagca
     3061 gcgtcacttt aaagcgaaca ggatgatgat cgacattacg taagggaaac gaggcataat
     3121 atcagagtga aagaacaagg gagcggacta ttaggaggga aacggtagaa attccctgat
     3181 caaaggaaca gaagtataag aaagaatcta caaccttaac ggctttcaag cacagccatg
     3241 gatagggatc tatttccgtc ttgcattgag gaatagaccg aaaggatggg aagtattctt
     3301 ttttatcaaa ggaattagaa gacagggcac atgagagtat atgaagtaat atcaaataag
     3361 atgcaaacaa acgtctacat aagggaagcc tcgagcttcc ctacagtgag cgcttctatc
     3421 agggtattct caatgtctgg gatttctgag ctaactctaa aaagtaggtg cagataacat
     3481 aatgacaggc atagaaaagc agagcagcat caatcgttca gcatggatta cgttggtaat
     3541 tctgagctgc cttggcctta taacaatgta tggagagaca atggtgctgc cagctattcc
     3601 agactttata cgggatttta atatctcgta caacacatcg tcatggatac tctcttctta
     3661 cctgattgca ggagcagtca tgacgccaat tgcaggcagg ttatcagaca tctatggcaa
     3721 gaaaaagata ttgcttatag tcatggcagt atactctaca ggcattttag caggaggatt
     3781 cgccaacagc attgaattca tgcttgcagc aagaacggca cagggtgttg ggatgtctat
     3841 gttcccaata gcctttggaa tcatcagaga aatccttcca gagaaaaagc ttgcaatagg
     3901 tcagactatt ttcagttcga cattttcggg tggggctgta gtggggttga tagttggtgc
     3961 aaccataatt caaaactttg gatggcaagc tacattcttt tcaatatttc ctatagcagt
     4021 catacttggc ctgataatca ggaaacttat caacgtcagg aatctggcaa cccaggaggt
     4081 gaaagtggag ggaggaggag aagtaagaaa tgatgagaat tcttctgtca agaaacagac
     4141 aatcgattta aaggggacag tagccttggc tgttacaata atttcatttc tagctggcat
     4201 ttccttcttg gaaagcaata gcaatgacgc aggatttcag gttgcaggac ttt
//
LOCUS       DASQTF010000132         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_677176,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000132 DASQTF010000000
VERSION     DASQTF010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677176"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1030
                     /locus_tag="VE308_03110"
     CDS             206..1030
                     /locus_tag="VE308_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99993.1"
                     /translation="MNLDNNNNVTLAQAHADKASSLLNNSTLDEIREVNNRIADSLET
                     GLEQLEGNVTAFPSASQGQIPQDRIQSINQTVVSLNDILAEAVTVRVESEQQNNATTW
                     AMALADLVNVVLSNYGNATGAPFDLTDMSNLAGMEGMQMSGNSSSNNTMTLTANATSS
                     MSNMTATIVNVEAYQSAQYLANNTTLQLFNDVLKPLTISDNETSSDNNNATPMAQQGQ
                     NNVTPDNNTTSNIDSLEANLLQLKDDINGRVTPAEIMTTVHLGIHPILMQLYGLTM"
     gene            1349..1759
                     /locus_tag="VE308_03115"
     CDS             1349..1759
                     /locus_tag="VE308_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99994.1"
                     /translation="MQKGRDLGPVQGSISKPVLLGLTTALVVGIVAPLVIPHATHPSM
                     IYHILLHMAGLTIAIFLSIVSSLAYSRNTTARMFLMAVGFMSLALVELFYLLQAGGIV
                     VGQFIIPAINIELSHMILLIMVSLFGLGVLKVNK"
     gene            1802..2278
                     /locus_tag="VE308_03120"
     CDS             1802..2278
                     /locus_tag="VE308_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZA99995.1"
                     /translation="MKHIDQTPGIRYRELLRLTGLVNGVLSYHLSALERANVIKVNRE
                     SRITRYYPVSVSDKESAILKFVRHEPVRQILIFILEHDMCTFNEIVDHTGKAPSTVSS
                     HLKRLREAGVVLVRHGEYHQLYHVVQGDLVSEVLSKYTAGFTDKVVDNYTGMLEEL"
     gene            2352..2864
                     /locus_tag="VE308_03125"
     CDS             2352..2864
                     /locus_tag="VE308_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99996.1"
                     /translation="MTMLATSVHVFADLLQGGSKQRIGNYDVEMTTEPKSPTVGDSPA
                     NILIRIAGVNGDHPADVPIMMRLVKDSVVLQTTNPIIVPSGHYIHEFTFAQAGRYVLY
                     VDLNDYIYSGEVLTFTFFINVSGPFDYLFIAVPSAAVVAVGIFSMLVFMKGKKKKKSE
                     TQKAGRTELK"
     gene            2935..3450
                     /locus_tag="VE308_03130"
     CDS             2935..3450
                     /locus_tag="VE308_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA99997.1"
                     /translation="MSKLPFIILAIIFVVIQTEVAYGHFFGATKNIDNYQVIFSPSPS
                     TPIAGDNSTSLNFSVLENNANIYNIYAALVVTEKQSADIIDQVPYKFEEFSDITIPYT
                     FNDTGTYVVTLQTRIVGDEKYQDSPMVASFDISVGNQLIPFDELMLFYVTPATAAIAG
                     IAIYLHSKKKF"
BASE COUNT         1109 a          739 c          768 g          987 t
ORIGIN      
        1 cccattgaca gcaaaaataa tgaggagaag gaaaatttca tttatgccgc attctatacg
       61 atttcttcta gtgtccttag tcctagccgg ctcttttata ttctcagcca gccacctggc
      121 atatgcccat acattctcaa ccagtgaagg tgctcacttc ctttcgttag ttgatcagat
      181 tagagctgag acaggattga taactatgaa tctggataat aacaacaatg taacactagc
      241 ccaagcccat gcagataaag cttcgagtct tctaaacaat tccactcttg atgagattag
      301 agaggtaaat aacagaattg cagatagtct tgaaactggc ctagagcagt tagaaggaaa
      361 cgtaacagca tttccttcag catcgcaagg gcagataccg caagacagga tacagagtat
      421 aaaccagact gtcgtatcgc tcaacgatat cttagcagag gctgtaaccg tacgagttga
      481 aagtgagcag cagaataacg caacaacatg ggcaatggct cttgcagact tggtaaatgt
      541 tgtattgagt aattatggga atgccaccgg tgcgcctttc gacctcacag atatgtctaa
      601 cttggcaggc atggaaggta tgcaaatgtc cggcaatagc agcagcaata acacaatgac
      661 tctgacggca aatgctactt cttcaatgag caatatgaca gcaactatag ttaatgtaga
      721 ggcctaccaa agcgctcagt atctggcaaa caatacaaca ttacaattgt tcaatgatgt
      781 gctcaagcca cttactataa gcgacaacga gacatcgagc gacaacaaca atgctactcc
      841 tatggcacaa caaggacaga acaacgtaac tccggataac aacacgacat caaacattga
      901 ttcgctagaa gcgaacctgc ttcagctaaa ggatgatatc aatggcagag taacgcctgc
      961 tgaaatcatg acgactgttc atcttgggat acatccaatt ctgatgcagc tttatggatt
     1021 aactatgtag catgaaggag gagatgaatc catggagaga tggatcagtg actcattcaa
     1081 gttttttctc aactccccgc gagtataaga aacataccta agtgaaaaaa gggtgccact
     1141 tgatagttgc atctccggct attgttgtct caagagtaac aacaacatag ttttgccttt
     1201 cagcggcctg ttttttgggc taataacatt caggaatgat gtccatgacc cagggcttta
     1261 gaaagcctcc tcttttcttt ttctcgttgt tcgtattcga ttcttgtacc aatttcttta
     1321 ttagccgttc aagggtatct aagatcggat gcaaaaaggg cgggaccttg gccctgtaca
     1381 gggatctata agcaagcctg ttctcttggg gcttacaaca gcacttgtag taggcattgt
     1441 tgcaccgctt gtaatacctc atgccactca cccttcaatg atttatcaca ttctattgca
     1501 tatggcaggt cttacgatag ccatatttct gagcattgtg tcgtcgctag catattcaag
     1561 aaataccacc gcaaggatgt tcctgatggc ggttggattc atgagtttgg ccttggttga
     1621 attattctat ctactccaag caggaggaat agtagtggga caatttatca ttcctgcaat
     1681 taatatcgag ctttctcata tgattctctt gataatggtt agcctctttg gacttggggt
     1741 gttaaaggtg aataaataaa gcatgcaagt aacagaacca gcggaaacca gggacttgct
     1801 catgaaacat atagaccaga ctccgggaat acgatacaga gagcttttga gattgaccgg
     1861 ccttgtcaat ggtgtccttt cctatcacct atcagcactt gaaagggcaa acgtgataaa
     1921 ggtcaataga gagtcaagga taaccagata ctatccggta agtgtctctg ataaagaatc
     1981 ggcaattttg aaatttgtta gacatgagcc tgtcaggcag atattgatat ttattctaga
     2041 acacgacatg tgcacgttca atgaaattgt agatcataca ggaaaggctc cttcaacagt
     2101 ttcatcacac ctcaagcggc taagagaggc cggcgttgtg ttagtcaggc acggtgaata
     2161 ccatcagctt taccatgttg tccaagggga cttggtttct gaggtgctct caaagtatac
     2221 tgcaggcttt actgacaaag ttgtagataa ttacactggg atgctagaag aactataata
     2281 gccggtatac actaatcaat aactgggact tgagaacagt tgtcctattc gtcttattat
     2341 tatttgtagg gttgacgatg ctagccacat ctgttcatgt atttgcagat ctgctgcaag
     2401 gagggtccaa gcaaagaata ggcaactatg atgttgaaat gaccacagag ccaaaaagcc
     2461 cgacagtcgg cgatagtcca gcgaacatcc ttataagaat agcaggggtc aatggtgatc
     2521 atccagctga tgtgccaata atgatgaggc tagtcaagga tagtgttgta cttcagacga
     2581 cgaatccaat aatcgtacct tctggacact atattcatga atttacattc gcacaagccg
     2641 gtagatatgt tctgtatgtt gacttgaacg actatatcta tagtggagaa gtcctgacat
     2701 ttactttttt tattaatgtg tcaggtccct ttgactatct gttcattgca gtgccttcag
     2761 cagcagtggt tgccgttgga atattcagca tgctagtttt tatgaaagga aagaagaaga
     2821 agaagagtga gacacagaaa gccggcagga cagagctaaa gtaggaagag gaatggaatg
     2881 taccacgtag tgttgaaata tgcatgagaa gtactataat cttgctagca aaaaatgtcc
     2941 aaactaccat ttatcatcct agcaattatc tttgttgtaa tccaaacaga agttgcttat
     3001 gggcacttct ttggtgctac aaaaaacatt gacaactatc aagtgatatt ttcaccaagt
     3061 ccctctacac ctattgcagg agacaattcc acatcgctta actttagtgt ccttgaaaat
     3121 aacgccaaca tttacaatat ttatgctgcg ctagtcgtga ccgagaagca atcggctgac
     3181 atcatcgacc aggtgccgta taagtttgag gagtttagcg acatcactat accctacacc
     3241 tttaatgaca cgggcactta tgtagtaacc ctgcaaacta ggatcgttgg cgacgaaaag
     3301 tatcaagaca gtcccatggt agcaagcttt gacatttctg ttggcaacca gttgatacca
     3361 tttgatgaat tgatgctgtt ctatgtcaca cctgctacgg cggctatcgc aggcattgcg
     3421 atatacttgc actccaagaa aaagttctag gcaattagat ccacccacgc acacaataca
     3481 aggcaaaatt aagctaagga gaaagaacaa ctaaattgaa aaaaagctat atcagtgcat
     3541 attattgtct ttggcttgat tagtaagcaa ttatatttat tttttgaatt gtccgcatac
     3601 ata
//
LOCUS       DASQTF010000133         7576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1050513,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000133 DASQTF010000000
VERSION     DASQTF010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050513"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..406
                     /locus_tag="VE308_03135"
     CDS             14..406
                     /locus_tag="VE308_03135"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZA99998.1"
                     /translation="MKEQYKFGTAINCIDGRTQQPVIDYIKQNYAVDIVDMITFPGAN
                     GIFSNELRHMEASLAKHSASISVEKHNSQIIAVVGHYDCAGNPGDKGYHYVQIRKALQ
                     EISLWKLPAKIIGLYVNGNWQVEKVEEE"
     gene            complement(463..1284)
                     /gene="uppS"
                     /locus_tag="VE308_03140"
     CDS             complement(463..1284)
                     /gene="uppS"
                     /locus_tag="VE308_03140"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZA99999.1"
                     /translation="MGFQTDIDRGGRALMDTFLKLSGIYSFYERHLELEIIKYPLPNH
                     IAIVLDGNRRWANMHLLDTTWGHNHGADKAEELLNWIHDVGIKITTLYVLSTENLERK
                     DEELENIYRLLGKKLEKLYNDERVHKRRMKIKAIGDTKLLPYNLQQILTKLEESTADY
                     DSMFLNIAIAYGGQKELIEAIRKIARMAEKGVIEVDEIDEKTIESCLYTSHLPQPSPD
                     LILRTSGEKRLSGFLIWQSAYSELMFMDVFWPEFRKIDLMRAIRTYQQRVRRYGR"
     gene            complement(1368..2168)
                     /gene="murI"
                     /locus_tag="VE308_03145"
     CDS             complement(1368..2168)
                     /gene="murI"
                     /locus_tag="VE308_03145"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZB00001.1"
                     /translation="MTNNPVAVFDSGIGSLSIIRELRREIPYEDLLYFADRVHFPYGT
                     KSHAELREIIVNTINYLKRYEPKLVVVASNTPSVQVLDEVRRTINDISIIGVRPPLKE
                     AARLTKKKHIGIMATEGMIRSREFENQIRREVPQHILVTRFNASPIIELIEQGVQLEN
                     ERRTFNVISRVLGDSRDEKIDVIILSSTHLPFVKRYLNSLLPTVRFIDPAQIVAKDVR
                     KFLAFYRMAKKSGRSNLRILVSDGKREFEKSARALGVREPIEEVFLTF"
     gene            2302..2454
                     /locus_tag="VE308_03150"
     CDS             2302..2454
                     /locus_tag="VE308_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00002.1"
                     /translation="MRSSIGNAVEISEPWIIATFVSAVVAIVILTVNEFAIEPRRWRK
                     NIESAI"
     gene            complement(2647..3117)
                     /locus_tag="VE308_03155"
     CDS             complement(2647..3117)
                     /locus_tag="VE308_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00003.1"
                     /translation="MQNDRTSAPIIRFSEFLGIGYFGDKKQRVIAPIFDLHSNAEQGW
                     KKVMTSHQQEVPIFHTIFVEHPIRYEFIAHPVEMSSERINYVLYRSSSSLSSLSKFKS
                     NYGSGMTYIKFGWHDLTKPHKFDVLPYYAVVDSIEFKKADDIKPGMLVDQIRKS"
     gene            3192..3608
                     /locus_tag="VE308_03160"
     CDS             3192..3608
                     /locus_tag="VE308_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZB00004.1"
                     /translation="MQYTTTAMFSRIGAVILLVSDIKRSIRFYRDTLGMELKQESKDW
                     TEFSTRGTVLALHPAKRKKIKKNNGMLVGFSISDFDDVINSLKKKRVKFYKKPKQEPF
                     GKHAIIQDPDGHLISIVQMPQEELSQIPYYHGFAPV"
     gene            complement(3587..4174)
                     /locus_tag="VE308_03165"
     CDS             complement(3587..4174)
                     /locus_tag="VE308_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00005.1"
                     /translation="MIVIDSYLTMILPDDIAERISEFIAGKRNFPFVGPDEMMCIMYL
                     YGRNSKIGEHEIDEVSSLAQYTASQLGQEIDIYINSSASKLETEYIRTKYINRELQLT
                     VEREPQDVKKRIAGDPVIILDCFAHHVAYYKQDYFLELYGPLKDSELTSDIRSAMAHR
                     MIMVCYNRKGAQETRLAHPLIPVYVWFKDQTGAKP"
     gene            4232..4525
                     /locus_tag="VE308_03170"
     CDS             4232..4525
                     /locus_tag="VE308_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00006.1"
                     /translation="MFEIDSTRKIHLIVHGQHQLIEPTKDKLAAAKFLRENMQPASLD
                     KAGDIGDYVAMLWPPMAPKEIVVSQITGTGSDSGQGMGAWASITQKELFRVPL"
     gene            4646..5293
                     /locus_tag="VE308_03175"
     CDS             4646..5293
                     /locus_tag="VE308_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00007.1"
                     /translation="MNAFDKNWSNQKRVSTGDKLKEALATQGPLKPRIETAANKLQLQ
                     ISKLDSIVTKLNERDASLFRRVVDAVQRHDTDTTKVLSNELAEVRKVSRTLGQAKMAF
                     EQVSMRLTTIHDMGDVMVALGPAITSIKSLKPGLGKFVPGADSEINTMQTLLNGIMME
                     SLQSSGSGIEVNTGSGSDIDQIMMEASAVAEQNMNDKFPSIPAGSFRISDTESQQ"
     gene            5300..5506
                     /locus_tag="VE308_03180"
     CDS             5300..5506
                     /locus_tag="VE308_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00008.1"
                     /translation="MVTRTGILAIFGAIGFAIGFLAFLASPSVASALATILHVDASIV
                     FALISGVAGAAISTSVITLWARRP"
     gene            complement(5513..6310)
                     /gene="cobM"
                     /locus_tag="VE308_03185"
     CDS             complement(5513..6310)
                     /gene="cobM"
                     /locus_tag="VE308_03185"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HZB00009.1"
                     /translation="MPETRTVYFVGSGPGDPELITLRAKKLVEEADTIIYSGSLLNPR
                     ILQYAKKGAQLYDAAIIDREKIYQILYDSAKEGKVVIRFHDGDPALFSAIREQIDKLE
                     SDGIKCKVVPGVTALFGAAADMNLELTLPGVTQTLIITRAELRTPVPERESIAELAKH
                     GATMAFYLSVHLIGEVVNELLKGGGVYTEKTPAAVVYRATWEDEKIIKGTLGDIAKRT
                     KEAKIIKTALIIVGEAIAPVKYEYSKVYDARFTHGYRKGAVEEKKKE"
     gene            complement(6297..7022)
                     /gene="cobI"
                     /locus_tag="VE308_03190"
     CDS             complement(6297..7022)
                     /gene="cobI"
                     /locus_tag="VE308_03190"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HZB00010.1"
                     /translation="MKLFCIGCGPGDPDLLTVRAADLIKSADMIFAPTAREGKSSIAL
                     SVVEKYIDKFAKTVSLVFPMVKNRESLKGYWERNADEIASAVRTGKKVVYLTVGDPAL
                     YSTWIYIQRELKRTHKDIEIEIVPGVTSMFAIAAKAGISLAEGDETVAIVPACYDLEK
                     VKRTASACDTVIFLKDGRYFDSVIDMLGEAGFPDSATVAIAQNVSEEGELLEIKKISE
                     LRGKKGPTEKYFSIMVAKKNARD"
     gene            complement(7058..>7576)
                     /locus_tag="VE308_03195"
     CDS             complement(7058..>7576)
                     /locus_tag="VE308_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HZB00011.1"
                     /translation="SYKQIKYAYRRLARKYHPDRNNSVYAEEMIKKINTAFEILSDET
                     KRAQYDKMEFDSSREQEPTQEPAHTNPKPWKMANESVIADVFNGQYLLDTPRGRFHII
                     VEPSLCMAFGSCEVLAPKVFVVEKNKMVNPKARVECETCADYETIHAAAATCPTKAIK
                     IIDRYTGDQLYP"
BASE COUNT         1976 a         1741 c         1646 g         2213 t
ORIGIN      
        1 tgattaatcc tcaatgaaag agcaatacaa atttggaact gctataaatt gtattgatgg
       61 aagaactcag cagcctgtca ttgattatat taaacaaaac tatgcagttg acatagtaga
      121 catgataact ttccccggtg ccaatggaat tttctcaaat gaattgcgcc atatggaagc
      181 atcactcgca aaacattcag catctatatc tgttgagaaa cataactccc agatcatagc
      241 agtagttgga cattatgact gtgcaggcaa ccctggagat aagggctatc attatgttca
      301 aatcagaaag gctctgcaag agatatcttt gtggaaactc cctgcaaaaa tcattggtct
      361 ctatgttaac ggcaactggc aagttgaaaa agtagaagaa gaataatcct atcttctgat
      421 tcgaaaatag tgacctcaac ttgaggttac tttagtgtta tattacctgc catatctgcg
      481 cactctctgc tggtatgtcc taatggctcg cataagatca atcttgcgaa actctggcca
      541 gaacacatcc atgaacatca gctcactgta ggcactctgc catatcaaga agccactcag
      601 ccttttttcg ccagaagtcc tcaggataag atcaggtgat ggctgcggta ggtgtgacgt
      661 gtacaggcaa gattcaatgg tcttttcatc aatttcgtca acttcaatca cgcccttctc
      721 ggccattcga gctatctttc tgatagcctc gatgagctcc ttttgaccgc catacgctat
      781 tgcgatattg agaaacattg agtcataatc ggcagtagac tcctcaagct ttgtcaatat
      841 ctgctgcaag ttatagggaa gcagcttagt gtcgcctata gcttttatct tcatcctgcg
      901 cttgtgcact cgctcgtcat tgtagagctt ttcgagcttc tttccaagaa gcctgtagat
      961 gttttcaagc tcttcgtcct tacgctcaag attttcagtt gatagcacgt acagcgtggt
     1021 gatcttgatg cccacgtcat gtatccagtt caatagttct tctgccttgt cggccccatg
     1081 gttgtgtccc caagtagtat caagcagatg catattggcc cacctcctgt taccatcaag
     1141 cacaattgca atgtggtttg gcagcggata cttgataatt tctaactcta agtgtctttc
     1201 ataaaacgag tatatcccgc tgagcttgag gaaagtatcc atcagtgccc tgcctcctct
     1261 atcaatatct gtttgaaatc ccatttttgc tcttagctgc ctgtctttta ctgtattgat
     1321 tattcctccc ttgcatatat ttagagtatg tatgttacta gtttgaatca aaatgtaagg
     1381 aacacttctt caatcggctc tcttactccc aatgcacgcg ctgatttctc gaattctcgc
     1441 ttgccatcag agactaggat ccgaagatta ctcctaccgc tcttttttgc cattcgataa
     1501 aatgcaagga acttccttac gtccttggct actatctggg ccgggtcaat gaacctaacg
     1561 gtaggcaaga gcgaattgag atatcgtttt acaaatggca ggtgagtgct tgagagtata
     1621 atgacgtcaa tcttttcatc cctgctgtcg cccagaacgc gggagatcac attaaacgtt
     1681 cgtcgttcat tctcaagctg caccccctgt tctatcagct caattatagg tgaggcattg
     1741 aaccttgtta ccaagatatg ttggggtact tccctgcgaa tttggttctc aaactccctg
     1801 ctccttatca tgccctccgt ggccatgatt ccaatatgct ttttctttgt gagtctagca
     1861 gcttctttga ggggtggcct gactccaatt atcgaaatat catttatcgt tcttcttacc
     1921 tcatcgagta cttgcaccga gggagtgttt gaggcaacta ctactagctt tggttcgtaa
     1981 cgcttgaggt agtttattgt atttacaatg atctcgcgca gctctgcatg tgatttggta
     2041 ccgtatggaa agtggaccct gtctgcaaag tagagcaggt cttcgtatgg tatctcgcgc
     2101 cttagctccc gtattatcga gagggatcca atgcctgagt caaacacggc tactgggttg
     2161 ttcgtcaatg cctttttttg atcatgatgc aaggtaaatt taaagcgtag gatatttttc
     2221 atggatagtc taaccgacga cccgtatgtt gaggctaaag cgggcggagc tgcaaattat
     2281 ggataactag atattagaag tgtgaggagc agcatcggga atgcggtaga gattagtgaa
     2341 ccctggataa ttgctacatt tgtttcagca gtcgtggcaa ttgtgatact aacagtaaat
     2401 gaatttgcta tcgagcctcg cagatggcgt aaaaatattg agtcagcaat ctagaaaaaa
     2461 ggccgtctag acctgcctcc attcactgaa tctactatgg ctagaagaat tccatcaaaa
     2521 tgctgtcagc ggctggatgt gtgctatcaa tcaaggaaag tgtcttaatg attatgattc
     2581 attaaaaaac catataacgg ggaccctgta cataaagaac aataacaaat attgtatgaa
     2641 ttcctgctag cttttcctta tttggtcgac tagcatccct ggtttgatat cgtctgcctt
     2701 cttgaactct attgagtcaa caacagcata atacggaagc acatcaaact tatgcggttt
     2761 tgttagatca tgccatccaa atttgatgta cgtcattccg ctgccgtaat tactcttgaa
     2821 tttgctcagg ctagacaggc tagaagatga acggtaaagc acgtaattga tcctttcaga
     2881 actcatttct acagggtggg caatgaactc atatcttata gggtgctcaa caaatattgt
     2941 atgaaagata gggacctctt gctgatgact tgtcattact tttttccacc cttgctctgc
     3001 attggagtgc aaatcgaaaa taggagctat aacgcgctgc tttttgtcgc caaagtaccc
     3061 tatcccaaga aattctgaga acctaataat gggggcagat gtcctgtcgt tttgcaagat
     3121 agtctgctga acctgtgatc gagatatata taatactaca tcgaccggca ttatcaactt
     3181 tatatttatg gatgcaatac actactactg caatgtttag ccggatagga gcggtcatct
     3241 tgcttgtttc cgacattaaa agatcaatca gattctatcg cgatacccta ggtatggagc
     3301 taaaacaaga gtcaaaggac tggacagagt tttcaacgcg tggcacggtg ctagcgctcc
     3361 accccgcgaa aagaaagaag atcaaaaaga acaatggcat gctggtgggg tttagcataa
     3421 gtgactttga cgatgttatt aacagcctga aaaagaaaag ggtcaaattc tacaaaaagc
     3481 ccaaacagga gccctttggt aagcatgcaa taatccaaga tcctgatggg catttgatat
     3541 ccattgttca gatgccacag gaggagcttt cgcagattcc atattatcac ggctttgcgc
     3601 cggtctgatc cttgaaccat acgtatactg gaataagggg atgtgccaat cttgtctctt
     3661 gagctccttt tctattgtag cacaccatga tcattctgtg agccatcgcc gagcggatat
     3721 ctgacgtaag ctcagaatcc ttaagcgggc cgtacagctc caaaaaatag tcctgcttgt
     3781 agtaagcaac atgatgcgca aagcagtcca atattatgac tggatcccct gctattcgct
     3841 tcttgacatc ctgtggttct ctttcaacag tcaattgcag ttctctattg atatacttag
     3901 ttctgatgta ttctgtttca agtttgcttg cggagctgtt gatgtaaata tcgatctctt
     3961 gccccagctg ggaggcagtg tactgcgcaa ggctgctgac ttcatcaatc tcatgttcgc
     4021 ctatcttgct gtttctgccg tataggtaca ttatgcacat catctcatcg gggcctacaa
     4081 agggaaagtt tcgtttacca gcaataaatt cagagatgcg ctctgcaatg tcatcgggca
     4141 agatcatggt caaataagag tcgataacta tcatgtgaga gaaaaactat ttaacgtgct
     4201 tatagttgta tgcatcttgc aacactcaac tatgtttgaa attgatagca caaggaagat
     4261 tcacctcatc gtgcacgggc agcatcagct catagagccc acgaaggata aacttgctgc
     4321 cgccaaattc ctgcgagaga acatgcagcc tgcctccctt gacaaggccg gtgacattgg
     4381 tgattatgtg gcgatgctgt ggccgcccat ggctccaaag gagatcgtgg tgagccagat
     4441 aactggcaca ggcagcgaca gcgggcaggg catgggcgca tgggcctcaa taacacagaa
     4501 ggaacttttt agggtaccat tgtgatattc aatagaattg ttcttcacaa gctcaccgtc
     4561 tctacaacta gaggataatt agtttagctt atggtttatc gaaatataca cctacaatta
     4621 atagcaaatc catgctcaaa tttctatgaa tgctttcgat aagaattgga gtaatcaaaa
     4681 gagggtaagc acaggagaca agttgaaaga agcacttgca actcagggtc ctctcaaacc
     4741 tagaatagag acagcagcta acaagcttca gttacagata tccaagttag actccatagt
     4801 taccaaacta aacgagaggg acgcatcttt gtttaggcga gtagtcgatg ccgtgcagag
     4861 gcacgacacc gatactacaa aggtgctctc aaacgagctt gccgaggtgc gcaaggtctc
     4921 gcgcacgctg gggcaggcta agatggcatt tgagcaggtc tcaatgagac taactactat
     4981 ccatgatatg ggagacgtaa tggttgcgct ggggccagca ataacctcaa taaagagtct
     5041 aaaacccggt cttggaaagt tcgtgcccgg tgccgacagc gagatcaata ccatgcagac
     5101 tttgctcaac ggcatcatga tggagtcttt gcagagcagc ggctctggaa tcgaggtcaa
     5161 tacaggctct ggcagcgaca tcgatcagat aatgatggaa gcttcggcag tcgcagaaca
     5221 gaatatgaac gacaagttcc catcgatccc ggctggcagc ttcaggatct cagacaccga
     5281 aagtcagcag tgatgtaaaa tggtaacaag aactggaata cttgcaatct ttggagcgat
     5341 agggtttgct atagggtttc tagcgttctt agcatcgcca tcggttgcaa gcgcattagc
     5401 aaccatcctg catgtggatg caagcattgt atttgccctg ataagtggag tggcaggagc
     5461 agccattagc acaagcgtta taaccttgtg ggcgagaaga ccgtaaaacc cttcattctt
     5521 tcttcttttc ttctactgcc ccttttctgt acccatgtgt gaatcttgca tcatacactt
     5581 ttgaatattc atattttact ggggcaatgg cctcgccgac aattatcagt gcagttttga
     5641 ttatcttggc ttcctttgtc ctcttagcaa tgtctccaag cgttcccttg attatttttt
     5701 cgtcttccca ggtcgctcga tagactacag cagctggcgt cttctctgta tatactcctc
     5761 ctccttttag tagctcgttt actacctcgc ctattaggtg cacgctcaga tagaatgcca
     5821 ttgttgcgcc atgtttggca agctcggcta ttgattcgcg ctctggaact ggcgtgcgaa
     5881 gctctgccct tgttattatc aaagtctgtg ttactcccgg cagcgtcagc tctaagttca
     5941 tgtctgcagc agctccaaat aaagcagtta cacctggcac aaccttgcac tttatgccgt
     6001 ctgactccag cttatctatc tgctctcgga ttgcgctgaa aagcgctggg tcaccatcat
     6061 ggaaccttat aacaaccttg ccttccttag cagagtcgta caggatctga tagatctttt
     6121 ctctgtcaat tattgcagca tcgtagagct gcgcaccttt cttggcgtat tgcagaatcc
     6181 tcgggttaag aagcgagccg gaataaataa tagtgtctgc ttcttctacc agcttttttg
     6241 ctctaagtgt gataagctca ggatcgccag ggcccgagcc cacaaaatac accgttctag
     6301 tctctggcat ttttctttgc caccataata gaaaagtact tttcagttgg gccttttttg
     6361 ccacgaagct ctgaaatctt ttttatctca agcaactcac cttcctctga aacattctga
     6421 gctattgcaa cagtcgcgct gtcgggaaag cctgcctctc ccagcatatc aattacactg
     6481 tcaaagtagc gtccatcctt tagaaaaatg acggtgtcac aggcgctggc tgtgcgtttg
     6541 actttttcca gatcataaca cgctggtaca atagctacgg tttcatcgcc ttctgccaag
     6601 cttatccctg ctttggcggc aattgcaaac atggatgtca caccgggcac gatttcgatt
     6661 tcgatgtctt tatgcgtcct tttcaattcg cgctggatat atatccaggt gctgtagagc
     6721 gccgggtctc cgactgtaag atatacgact tttttgccgg ttctgactgc acttgcaatt
     6781 tcgtcagcat ttctctccca gtagcccttg agcgattccc tgttcttgac catcggaaaa
     6841 acaaggctca ctgtcttggc aaacttgtca atatattttt caacgacaga aagcgcaatg
     6901 ctggatttgc cctcccttgc agtgggtgca aatatcatat ctgcgctttt tataaggtct
     6961 gctgcccgca ccgtgagaag gtctgggtct cctgggccgc atccaataca aaaaagcttc
     7021 atggattgca gcacaatcac agagcaattt aaatttacta tggatagagt tgatcgccag
     7081 tataacggtc aatgatcttt atggccttag tcggacaggt ggctgcagca gcatgtatgg
     7141 tttcatagtc tgcgcaggtt tcgcactcga ctcttgcctt cggattgacc atcttgttct
     7201 tttccactac aaaaactttt ggcgcaagaa cttcacagct gccaaacgcc atacatagcg
     7261 aaggctcgac aattatgtgg aatctgcctc ttggtgtatc aagtaagtat tgcccattga
     7321 aaacgtctgc aatcacgctt tcgtttgcca ttttccatgg cttgggattt gtgtgcgctg
     7381 gctcttgggt tggctcctgc tcacgcgagc tatcaaattc catcttgtcg tattgcgccc
     7441 tctttgtctc atctgacaga atttcaaacg ccgtgtttat tttttttatc atttcttcag
     7501 catagactga gttgttccta tccggatgat attttcgggc aagccgccta taggcgtact
     7561 tgatctgctt gtagct
//
LOCUS       DASQTF010000134         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_401974,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000134 DASQTF010000000
VERSION     DASQTF010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401974"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(472..870)
                     /locus_tag="VE308_03200"
     CDS             complement(472..870)
                     /locus_tag="VE308_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HZB00012.1"
                     /translation="MIKNRIDVMLKMVEMDENVTLAAQLEEQRNGQSVILINKFNVKP
                     EDVDSLLKAWAADAAYLKQKSGFISTQLHRGIGGSCVFINYAVWESVEHFKQATSDPA
                     FRSALARYPDSTVGSPHLFRKVAVPGICVD"
     gene            complement(956..1507)
                     /locus_tag="VE308_03205"
     CDS             complement(956..1507)
                     /locus_tag="VE308_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00013.1"
                     /translation="MAFAAVLGVIFSISAVDSFAQTDLTTAGTTPGEERNATFATASL
                     GNPFFVEQGRIIGQRVLAVTPQPQLEFSFMANASINNGGGNAVINAINTGTSVSTLNA
                     DGTFRGEGQGILRTQGGDMATWTNQVVGNITPEGTIITRGVGIWSTPSTTGELGFMNN
                     MITVFEVQIDREGNLSAREWEWR"
     gene            complement(1751..2170)
                     /locus_tag="VE308_03210"
     CDS             complement(1751..2170)
                     /locus_tag="VE308_03210"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZB00014.1"
                     /translation="MVHQFFEAFDRHDIESMEQLVSITNYSLHFSGMPTMDWNGHKQL
                     IAAIITAFPDIHHDIEDVVAEGADKVAIRMNVTGTHKGEFQGVPQSGKKGSITSMDFF
                     TIIDGKFVEHWVTADMMGLMQQIGAIPASPSPSDATN"
     gene            2869..>3459
                     /locus_tag="VE308_03215"
     CDS             2869..>3459
                     /locus_tag="VE308_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00015.1"
                     /translation="MNKSQKKKQRNNEVKQRLALGLLVVAGFIGTLFAAPVLATVAES
                     FLVIEKARVTVDDDDEELTTELDVEGLQIPTDGSEGAFGYGILTKDGDAILVSTTHAE
                     VLDSEAQRFIEDPIWHNHFVKLGDMEQCGEDQGLVDITWQSPGEVGIDDNIATISDVP
                     TDEFEGWDSITGEPLSMTLGEAVSEVISFKLDPVFDE"
BASE COUNT          982 a          744 c          689 g         1044 t
ORIGIN      
        1 tttggctgac aggaatctat ctgagcataa gacaaggata atggattatg gcgctgatga
       61 atttgccgta aagccattga gcatggaatc tttgattaac aaagtaaata taaagttgct
      121 tgaaagtgca aaaagtgcag ctcctctttc ttcctaaaag tttctataca attctcaatc
      181 tctgcgccac ttacggcggc gttggcattg caagacattt tgtcctacaa ttgcctaaaa
      241 caagctcaat ttgataccgt agttctctat agtagtaaaa atggacgggg tgggagttga
      301 acccacgacc tcagccagct ctcagtaaag gcagttctat ctcttgtcta aatgggaagc
      361 agtattaaaa agagaacata actgttcaaa tccaacccgg tccactcttt ttcttttgca
      421 tgctctccat agccatccat gcgttttcaa gcctcgtttg gttgcctcaa ttcagtccac
      481 acaaattcca ggaacagcaa cttttctaaa gagatgagga gatcccacag tactgtcagg
      541 ataacgtgca agcgccgacc tgaatgcagg atcgctggtc gcctgcttaa agtgctccac
      601 agactcccat actgcataat tgataaacac gcaacttcct ccaatgcccc gatgaagctg
      661 tgtagagatg aaccctgact tctgctttag gtatgctgcg tctgctgccc aagctttgag
      721 caggctatcc acatcttctg gcttgacatt gaatttgtta atcaagatta cagattgccc
      781 attcctctgt tcttctagtt gtgcagcaag tgtcacattt tcatccattt caaccatttt
      841 taacataaca tcaatacggt tttttatcaa aaaaagatta tgcattggat caagatccta
      901 ttgcctccag cccccaaggt ttcaaagata tcagccatac ttgctttttg tcctatcatc
      961 tccactccca ttctctggct gatagattac cttctctatc tatctggact tcaaaaacag
     1021 ttatcatgtt attcataaaa cctagttcgc ccgttgttga aggtgtactc cagattccta
     1081 caccgcgggt tattatggtt ccctcaggag tgatgttccc tactacctga ttggtccaag
     1141 ttgccatgtc accaccctgt gtccttagta taccctgtcc ttcaccacga aatgttccat
     1201 cagcattgag tgtgcttacg cttgtcccag tatttattgc atttattaca gcattaccgc
     1261 caccattgtt gatactagca ttagccatga aactgaattc taactggggt tgtggggtaa
     1321 cagcaagtac tctttgtcct atgattctgc cttgctctac aaaaaatgga tttcccagcg
     1381 atgcggtggc gaaagtagca ttcctttctt cacccggggt agtaccagca gttgtcaagt
     1441 cagtctgagc aaatgaatcg actgcgctaa tgctaaaaat aactcctagg accgctgcga
     1501 atgccacaaa ggagatggct agaataattc tatcaaattt tctactgata ctcattgttt
     1561 gaacaaactc actgttattc aagttaagca tttcaccagc ttcatagcga atggtcttat
     1621 gataaaagca tcacttctag ttagcccaga cgtttttgta atccgagcat gtacttctat
     1681 cagtaactat tgttggctac tatgctacgc ggatgttaat ccatcatatc aagagcgagc
     1741 cgtaccgctg ctagttagta gcatctgatg gtgatggtga agcaggtatc gctccaatct
     1801 gctgcatcaa tcccatcatg tctgcagtta cccaatgttc tacaaatttg ccatcaataa
     1861 tagtaaagaa atccatcgaa gtgatagatc ctttcttgcc cgactgcggt acaccttgga
     1921 actctccttt atgagtgcca gtaacattca tacggattgc gactttatct gctccttctg
     1981 ccactacgtc ttcgatgtca tgatgaatgt ctggaaatgc agtgatgatg gcagcaatga
     2041 gttgcttgtg tccattccaa tccattgtag gcattcctga aaagtgaagg gagtagttag
     2101 tgatcgatac taactgctcc atactttcta tatcatgtcg atcgaaagcc tcaaagaact
     2161 gatgaaccac ctgtttattt ttttgttgtt cttgtcatga tgatgatgat gccattgttt
     2221 gggtacacga attgttcgca gtttaaacgt ttttggtttt tttatcgtca aactttccta
     2281 gtattaaaga cattctgaag aattatagat ataaccgctt gttctacttc ttgtcttcaa
     2341 aaacgtgtag ccttttctct actacttttg ttcttgtatc cttcctattc ttctaccgct
     2401 aatcctttta tcttgataat tatctgctca acttgacaaa tgtaatccat aagttgctcg
     2461 tagcggtaga gcagacataa tcagtattgg ataaaggctt acatgtggta cagcaaaaga
     2521 gagcaagctg agagcctagg actctcaaac accatctcat caaaaacttc tttctatcgg
     2581 atttacctaa ggcattgcta gaggttttgt ccgacagcta ttcaaatcag cccaatatga
     2641 tatcatagtt ctgtaatagt aagaaatgga ccgggtggga gttgaaccca caatctcaca
     2701 tcagcaataa ctttcttagg tcgctcctta ttccctatgt aaaggagggc agatgtggaa
     2761 ttgggatttt aataactata tccaaaatat tgtgaaattc acctatgaat tgcattccta
     2821 gcttttgaga tctgcttaaa taagtagttt attatataat ttataagatt gaataagtcg
     2881 caaaagaaaa agcaacgaaa taatgaagta aagcaaagac tagctctggg attacttgta
     2941 gtagcgggat ttattggaac actatttgca gcgcctgttc ttgcaactgt agcagaaagc
     3001 ttcctagtta tagaaaaagc tagagttacg gtagacgatg atgatgaaga actcacgact
     3061 gaattggacg tcgaaggact acaaattcca accgatggaa gcgaaggagc atttggttat
     3121 ggaattctca caaaggatgg ggatgcaatc ctagtttcta ctacacacgc agaagtactg
     3181 gatagcgaag ctcagagatt cattgaggat ccaatctggc ataaccattt cgtgaagctt
     3241 ggcgatatgg agcagtgtgg ggaagatcaa ggattagtag acatcacttg gcagtctcct
     3301 ggagaggtgg gaattgacga taacatcgca acgatcagcg atgtaccaac tgacgaattt
     3361 gaaggatggg acagcataac tggcgaacca ctatcaatga cgctgggcga agctgtatct
     3421 gaggttattt cattcaaatt agacccagtc tttgatgaa
//
LOCUS       DASQTF010000135         5886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_952052,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000135 DASQTF010000000
VERSION     DASQTF010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952052"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..912)
                     /locus_tag="VE308_03220"
     CDS             complement(271..912)
                     /locus_tag="VE308_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00016.1"
                     /translation="MMKFALYVAAAATTAIAGMLHLMLGPNNLDSNINQGILFIVGGI
                     AQVFWIIPMVRRWGIPWYAIGIGGTIVFMALWIITRIPGNPITGRGGPAGNPIAITIE
                     VLQAVFIGLAAAIIIYEHRKKMQQLPHDEEKHEAEKSTKNSDKRHMPILAGIVIALIL
                     IGLFALPLLMPSPMGGGGPPRQGGASPAGAGQLGAPQGQAPTQQNAMSTVSPT"
     gene            complement(1034..1399)
                     /locus_tag="VE308_03225"
     CDS             complement(1034..1399)
                     /locus_tag="VE308_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZB00017.1"
                     /translation="MSTGEIDPGLDYEGVLSNVFSSRAVSQIMDFFLDYKEFDYSPGE
                     IAKKTGLSFRTIFRELPYLEKNQLIYISRKIGKTNMYRLNTDFQAVTFLEKFAFEMSQ
                     IQIKESHSYLNKQNIPEIK"
     gene            1697..2122
                     /locus_tag="VE308_03230"
     CDS             1697..2122
                     /locus_tag="VE308_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00018.1"
                     /translation="MSINRERRKEILQIVDSDIDWHKLEAPRTKENYNSIPIKEKKSF
                     GQIITRYPISQAIRTIAASSLLIVGLVIALGSSMPTTSPDTESVAYPQYMPHKKWSGA
                     YGSDDHDGIKDPAPPSYSPSSASFVALKDCNQKLSQQQT"
     gene            2275..2877
                     /locus_tag="VE308_03235"
     CDS             2275..2877
                     /locus_tag="VE308_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00019.1"
                     /translation="MSMPHDAVMRNKQFVMFSGVAIVLAGLFAAMSLHEILAQETNST
                     AQSNQTRTGVPQLNGSVNVREQANQFIQDSVQVPFATALESAQAEVGNGTAISGHLGV
                     VQGYLVYIFKIANFDAETSRIVIVDAGNGAVLYTSGDMPSYFGGGGPGCGFGGGGHHH
                     KGFGNHWGNHDRSGDETSDRSNSGGQSPSSGLVVSPAIGV"
     gene            3059..3502
                     /locus_tag="VE308_03240"
     CDS             3059..3502
                     /locus_tag="VE308_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZB00020.1"
                     /translation="MVFENIPTGYELRFLVDIGIALAAGFAIGAERESRGKPAGISTH
                     CLVIGGTAIFTFLSSVVDPNSSSRIAAQIVSGIGFLGAGIILKSEIDKKITNLTTAAA
                     IWFAASIGMAIGYEYYFLAVVATAFAVGVPRIPHISKLIKKGEEE"
     gene            3892..4254
                     /locus_tag="VE308_03245"
     CDS             3892..4254
                     /locus_tag="VE308_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZB00021.1"
                     /translation="MPFNPSNMEQVIISELKISEDEAKVYITIVKKGKMDPAKISGAL
                     GWSNEQRALTAAKSLVNRGMVIEISKTEFESLHPRFAITNRYRRRCEEERIPFKKNLK
                     IDNIGIVLEKPYEDARTK"
     gene            4288..4704
                     /locus_tag="VE308_03250"
     CDS             4288..4704
                     /locus_tag="VE308_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00022.1"
                     /translation="MTTSDPTGGDKVVYFGVININENGRTMGSVDIWRSLLTKELFCE
                     EKRLGVLEIVDYLGMPKIDKDKKWAVAISRNLKGHLRWRLIKMIEEGRMTFTNADDNP
                     VEVNVKNFKIVDPEWWSFLVEENINRNMEVTDAAKK"
     gene            4701..4997
                     /locus_tag="VE308_03255"
     CDS             4701..4997
                     /locus_tag="VE308_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00023.1"
                     /translation="MMRDEDIPVTVFVKSASGSEIASKLSGYFVVKGQEFRFTAIAFG
                     RIGGHNASIKISKSTLDKIAKMGVDPEQLQITIQRKLIEGDIILPEGSKPPPSD"
     gene            complement(4989..5786)
                     /locus_tag="VE308_03260"
     CDS             complement(4989..5786)
                     /locus_tag="VE308_03260"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZB00024.1"
                     /translation="MENSVDIGIIGGTGIYDPELFSAKREIKVHTPYGDASDLVIIGE
                     YSGVKAAFVPRHGRGHRIPPHKINNRANIWALEQLGVKRIIAPSAVGSLKEAFKPGDI
                     SVPDQFIDFTKNRQYTFYDGGQVCHVSLADPFCPELRNIAIDKIKKLKYSLHSEGTYI
                     CVEGPRFSTRAESRFFRDVMKADIIGMTLVPEVNLAREAEICYVSIATITDYDVWANH
                     PVTSTEIIETLAKNVEKTKKLIAELVATIPAVRNNCSCSKTLEDALV"
BASE COUNT         1728 a         1247 c         1267 g         1644 t
ORIGIN      
        1 aatgggctca tttgctgtcg aagactttgg gattaagcgc ctcctgtcga taacaaagga
       61 cgatgtggaa gaccgctacg aaaaatattg caatctggtg caattctaat ctcttgtttt
      121 attttgctca gagatagtaa cgatgggtat atttctgcat gtgcagtatc ggctttcact
      181 atgttaaacc tattgacatt caaaaatata acggggcgcg gaacctcctt agtaactact
      241 ttcgcttcat caatagcatt gcgactattc tcaggttgga ctcactgtac tcatggcatt
      301 ctgctgtgtt ggagcttgcc cttgcggagc tcctaactgt cctgcgccag caggggatgc
      361 gcctccttgt ctggggggac caccaccacc cattggacta ggcatcaaca gtggcagcgc
      421 aaacaaccca atcaatatca gtgcgataac tattccagca agaatgggca tatgtctctt
      481 gtcgctgttt tttgttgatt tttctgcctc atgcttctct tcgtcatgcg ggagttgctg
      541 catcttcttc ctatgttcgt agattattat tgcagctgca agccctatga atactgcctg
      601 caatacttca attgtaatag ctattggatt gcccgcaggg ccacctcttc cagttatggg
      661 attgcctggt attcgtgtta taatccaaag agccatgaat acaatggttc ctcctattcc
      721 tattgcgtac caaggtatgc cccatcttct taccataggt atgatccaga atacttgagc
      781 aattcctcct acaatgaata gtatgccttg attgatgtta gaatccaggt tgtttggacc
      841 caacatcaga tgtagcatgc ctgctatcgc agttgtggca gctgctgcaa cataaagcgc
      901 aaacttcatc aaatctgcaa acttacaacc tgttatttat gttttgacaa atttgtcact
      961 atctaagaga aaaatagtct tttcatcaag aagggtaatg tagtttgtgc ctgttccaca
     1021 ccttgaacga acctcactta atctcaggta tgttctgttt atttaagtat gaatgagatt
     1081 ccttaatctg aatttgtgac atctcgaatg cgaacttctc cagaaaagtc actgcttgaa
     1141 agtcagtatt cagtctgtac atgtttgtct taccaatctt tctgctaatg taaatcagtt
     1201 ggtttttctc aaggtatggt agctctcgga agatggttct aaatgacaat cctgttttct
     1261 ttgctatctc tcccggcgaa taatcaaatt ccttgtagtc aaggaaaaag tccataattt
     1321 gggagacagc ccttgaagaa aacacattgg aaagtactcc ctcatagtct agacctgggt
     1381 caatttcgcc agtggacaca acgtaaattg aatacggaca tatttgttcc ttgctgttgc
     1441 aaagaatact tctttctaca tcaaatagtc gttgttgtct ggataaaaag attaataacg
     1501 caactgctta agtatcgttg tgaatacctt tgaggttgct gtactacgta ttcttatcaa
     1561 gaggcagcag ccactcaaat tatctactct agttagcggc tttcctgata actgtgagga
     1621 tagcgttctt tctgcaatct ctagcctaag gcttcacgga tatattttgt taaatgatta
     1681 tcaacctaat ggatatatgt caataaatag agagagacga aaggaaatcc tgcagattgt
     1741 cgattcagat atagattggc ataaattgga agccccacgt acaaaagaga attataatag
     1801 tatccctatc aaggagaaaa aaagttttgg tcagatcatt acaagatatc ccatctcgca
     1861 ggccataaga actatcgccg cttcttcttt gctcattgtt gggctcgtta tcgctctagg
     1921 aagtagtatg cctacaacaa gtccagacac agaatctgtc gcttaccctc aatatatgcc
     1981 tcataagaag tggagtggtg cttacggatc agatgatcat gatggtatca aggatcctgc
     2041 tcctccgtct tactcgcctt cctctgcatc attcgtggct ttgaaagatt gtaatcagaa
     2101 actatcacaa cagcaaacat aacatgaatt cactcgatgc atacaatcct atatttgaac
     2161 gtgtcattat tatgacacta tatcgagcca gtattgggac tttgtgctct ccagttaacg
     2221 ctattacatg ccaaatatag tacattaaaa agacattttt gtcaacatat atatatgtca
     2281 atgcctcatg atgcagtaat gaggaacaag caatttgtta tgttcagtgg cgtagctata
     2341 gtactagctg gcttgtttgc cgctatgtcg ctgcacgaaa tattagccca agaaacaaac
     2401 agcactgccc aatccaacca aacgaggaca ggagttcccc agctcaatgg ttctgtaaac
     2461 gtaagggagc aagcgaacca attcatacaa gatagtgtgc aagtgccttt tgctactgca
     2521 ttagaaagcg cacaagctga agttggaaat gggactgcaa tttcaggcca cctaggagtc
     2581 gtccaaggat atttagttta catcttcaaa attgcaaact ttgacgctga aacaagccgt
     2641 attgtaatag tcgatgctgg aaacggtgca gtattgtaca catctggaga tatgccatca
     2701 tactttggtg gtggtggtcc tggatgcggc ttcggcggtg gtggacatca tcacaagggt
     2761 tttggcaatc attggggaaa tcatgacaga agcggcgacg aaacatcaga tagaagcaat
     2821 agcggcggcc aatcccccag cagtggactg gttgtatcac cagccatcgg tgtgtgatag
     2881 cacgaccatt agacacattt ctttttccat tctgctggat gtataacact attgaccagt
     2941 acggccctag ctagccttta ataggatcat catgacattt agtttgacat gcaaaatcga
     3001 ctacatcaaa atggtgtcga tagaggccaa tcatgatata tagttagaaa tcaaaaaatt
     3061 ggtttttgaa aatataccta caggatatga gctacgattt ctagtggaca ttggcatcgc
     3121 acttgctgca ggctttgcaa ttggcgcaga aagagaatca aggggcaagc ctgctggaat
     3181 cagcacgcac tgccttgtca ttggcggtac agcaatattc acattcctct cttcagtggt
     3241 agatcctaac tcttcatcta gaattgcagc gcagatagtt tcgggtattg gctttcttgg
     3301 agccgggatc atcctcaaga gtgagataga caagaaaatt acaaatttga ctacagcagc
     3361 tgccatatgg tttgcagcat caattggaat ggctattggc tatgaatact attttctagc
     3421 agttgtagcc accgcgtttg ccgttggagt gccaaggata cctcatattt caaagttgat
     3481 aaagaaaggt gaagaagagt aatgaaatta ggtctttggc ttcttctgcc tcatatgcag
     3541 atgctctcaa ggtgcagact acctaggagc ttggggacat agagctactc gcaatcagga
     3601 tttaatatct agatgatatc tcacaaagcg agccggcaga gttgacaaac tatgagataa
     3661 ttagtctgat tggtcttgac aaaacaacta atccttaatt aaaaaggtta aagcagatcg
     3721 attttgaggg cgccagtaga gaactacatt aatttggcaa aaaccactaa aactgggata
     3781 acatgtcaca tataacctga caaccagcat tcggataatc ctatgaaagt caacatatgc
     3841 actctgaaga tatatttaac tcaagtcgct tgaattacaa agaaaactaa catgccattc
     3901 aatccatcaa acatggagca agtcatcatt tcagaactta agataagcga agacgaagcc
     3961 aaggtttaca tcactattgt gaaaaaaggc aaaatggacc cagccaagat ttcaggtgca
     4021 cttggctgga gtaatgagca gcgtgcactt actgcagcaa aatcgctagt caacagaggc
     4081 atggtcattg aaatatcaaa gacagaattt gagtcccttc acccccggtt tgcaataacc
     4141 aatcgctaca gaaggcgttg tgaggaagag cgcataccct tcaaaaagaa cctcaagatc
     4201 gacaacatcg gcatcgtgct tgaaaagcca tatgaggatg caagaactaa ataagtggca
     4261 acatccatca aggaataata tattatcatg acgacttcgg accctactgg cggcgacaag
     4321 gtagtctatt ttggtgtaat aaatatcaac gagaatggca ggacgatggg ttcagtggac
     4381 atctggcgaa gccttctgac aaaggaattg ttttgtgaag aaaagcgttt aggggtttta
     4441 gaaatcgtcg attacctcgg tatgcccaag attgataaag acaaaaagtg ggctgtggca
     4501 atcagcagaa acttaaaagg tcacctgcgc tggcggctta tcaagatgat agaggaaggc
     4561 aggatgacat ttacaaatgc agatgacaat ccagtcgaag tgaatgtcaa gaactttaaa
     4621 attgttgacc ccgagtggtg gagctttttg gtcgaagaga atatcaaccg taacatggaa
     4681 gtgacggatg cagcaaagaa atgatgcgcg atgaagacat tccagttaca gtgtttgtaa
     4741 aaagcgctag cgggagcgaa atagcaagta agctctctgg gtattttgtc gtcaagggtc
     4801 aggaattccg ctttactgca atagcatttg gtcgcattgg aggtcacaac gcaagcatca
     4861 agatatccaa gagcacttta gacaaaatag ctaaaatggg ggttgacccg gagcagctac
     4921 aaataacgat tcagcgcaag ttaattgaag gtgacattat tttgccggaa ggatcgaagc
     4981 cgcccccgtc agactagagc atcttcaagt gtcttgctgc agctacagtt gtttcttact
     5041 gcaggaattg tagccaccaa ctctgcaatc aatttctttg tcttttccac attcttggca
     5101 agcgtctcga ttatctcggt agaggtcaca ggatgatttg cccaaacatc atagtcggta
     5161 atggtggcta tagatacgta gcagatttca gcttctctcg caaggttaac ctccggtaca
     5221 agcgtcatac caataatatc tgccttcatt acatcgcgga agaatcttga ttcggctctg
     5281 gttgaaaagc ggggtccttc aacgcagatg tatgttccct cactgtgcag tgagtacttt
     5341 agtttcttta ttttatcgat cgcaatgttg cgtagctctg ggcaaaatgg atcagctaaa
     5401 gaaacatggc atacttgccc accatcgtag aacgtgtact ggcggttttt tgtaaagtca
     5461 ataaattgat caggaactga aatgtcgccc ggcttgaaag cctctttaag gcttccaaca
     5521 gcagatgggg caataatgcg tttgacccca agctgctcta aagcccatat gtttgctcta
     5581 ttgtttatct tgtgaggggg tatcctgtgg cctcgtccat gacgcggtac aaatgctgct
     5641 ttgactcctg aatattcgcc tattatcaca agatcggaag cgtcgccata aggcgtatgc
     5701 accttgatct cgcgttttgc gctaaaaagc tccggatcat aaatgccagt acccccaatt
     5761 atgccgatat ccacgctatt ttccatcaaa agtcaccaat gactccacat tatagcccat
     5821 cttgcgcaac ctgtcagcac ccttcaagtc tgaaagttca attatgaacg caaagccagc
     5881 cacctt
//
LOCUS       DASQTF010000136         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_222981,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000136 DASQTF010000000
VERSION     DASQTF010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222981"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1326
                     /locus_tag="VE308_03265"
     CDS             112..1326
                     /locus_tag="VE308_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZB00025.1"
                     /translation="MIKIGLIGKTNTGKTTFFNSATLMSAEVSNYPFTTKKPAMGNAH
                     AITHCVHKEFNLRDNPKNSGCIDGWRFIPVELVDLPGLIKGAWEGKGLGNQFLTIAAQ
                     SDALLHIVDASGSIDASGKIAEPGTGDPVADVADIEEELVMWYLKLLEANRDKISRAI
                     GSGMNTVLAITEVFRGIGVKEDHVKLALTQNNLTATIFDNFGTQESKDFCWSLREISK
                     PTLIVANKVDLPTATENFKRLREEYHDVIIIPASADAELTLRRAENKGIIRYIPGDER
                     FEISDQSKLNDKQKWALNFIRKDILGEYMRTGVQFAINVAVFKLLKMNAVYPVADVQK
                     FSDKHGNVLPDVYLMRSGSTVEDLAKEIHSELAKGVIYALDGRDGIRLPVNYQLKDRD
                     VLSIVSARTKKK"
     gene            1332..1574
                     /locus_tag="VE308_03270"
     CDS             1332..1574
                     /locus_tag="VE308_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00026.1"
                     /translation="MTSTELMQEIQNILCREPSRMYSREEILNLLAPKKKDHEIERLL
                     AELEVSTSLREGESDVYSTCRGGTVYYKWNNNNKSS"
     gene            complement(1571..1843)
                     /locus_tag="VE308_03275"
     CDS             complement(1571..1843)
                     /locus_tag="VE308_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZB00027.1"
                     /translation="MKNRSRPDIMAIILESANGGITKTKLLTRANLTSGQLRQYLDVL
                     ITKKLVVQLADEEKRRMAYRTTEKGIRYLSIYSTLKSIAVFQQEED"
     gene            2042..2302
                     /locus_tag="VE308_03280"
     CDS             2042..2302
                     /locus_tag="VE308_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00028.1"
                     /translation="MQKKKKKKKKNQLKYFDKSIVVVIDNGIEISDKDLLQVNATIIT
                     CGPIFLTLAASFLSSFDRKFSSTALFIKAGATLSFIILSFFS"
BASE COUNT          796 a          544 c          604 g          695 t
ORIGIN      
        1 cacctatcgt tgcttggagg gtttctaggt ttagtagggt accttgcaat cgtctgccac
       61 cacatccact gagcacagtg tatataaacg atgaaaatag agtgaaaaac catgatcaag
      121 ataggcctga ttggcaagac aaacacaggc aaaacgacgt tcttcaattc cgcgacactg
      181 atgtctgcgg aggtatccaa ctacccattc accacaaaga agcctgcaat gggcaatgct
      241 catgcaataa ctcactgtgt tcacaaggag ttcaacctgc gcgataatcc caagaactct
      301 ggatgtatag atggctggcg ctttattcca gttgaacttg ttgacctgcc tggtctaatc
      361 aagggtgcat gggaaggcaa gggacttggc aaccaatttc tcacaatagc agcacaatca
      421 gacgctttgc tgcacatcgt tgacgcatca ggcagcatcg acgcatcagg caagatcgcg
      481 gagcctggca cgggcgaccc cgttgcagat gttgcagaca tcgaggagga gcttgtcatg
      541 tggtacctaa agctgcttga ggcaaacagg gacaagatat ctcgagccat tggctcaggt
      601 atgaacacag ttcttgccat aacagaggtt ttccgcggca ttggggtaaa agaagatcat
      661 gtcaagcttg cccttacaca gaacaatctg actgcgacta tttttgacaa ctttgggaca
      721 caagagagca aggacttttg ctggagcctg cgagaaattt caaagcctac tctgatagtt
      781 gcaaacaagg tcgaccttcc tacagcaacg gaaaacttta agcgtctgcg cgaagaatat
      841 catgacgtta taataatacc cgcaagtgct gatgctgagc ttacacttag gcgtgcggaa
      901 aacaagggga tcattcgata cattcccggt gacgagcgct tcgaaataag tgaccagtca
      961 aagctcaatg acaagcaaaa gtgggcactt aacttcatac gcaaggatat cctaggcgaa
     1021 tatatgcgca ccggcgtcca gtttgcaata aatgtggctg tattcaagtt gctcaagatg
     1081 aatgccgttt acccagttgc tgacgtccaa aagttctcag ataagcatgg gaacgtgctg
     1141 ccagatgtat accttatgag gtcaggatct actgtagagg atctggcaaa ggagatccat
     1201 agcgagctgg caaagggcgt aatatatgct cttgatggca gggacggcat caggttgcct
     1261 gtaaactatc agcttaagga ccgtgatgta ttgtctatag tatcggcaag gacaaagaaa
     1321 aagtgattaa catgacatcg acagagctaa tgcaggaaat acaaaacata ctgtgtcggg
     1381 agccttcaag gatgtactca agggaagaaa tcctgaacct ccttgcgcca aagaagaagg
     1441 accacgaaat cgaacgattg ctggcggagc ttgaagtttc aacttcgctt agagaaggcg
     1501 aatctgatgt ttattcaacg tgtcgaggcg gcacggtcta ttacaaatgg aacaataaca
     1561 ataaaagtag ttaatcttct tcctgctgga agacggcgat gctttttaga gtggaataga
     1621 ttgacaggta tcttatgcct ttttccgtgg tccggtaagc catacgcctt ttctcttcgt
     1681 cagcaagttg cactaccagc ttttttgtaa ttagaacatc gagatactgt ctaagctgtc
     1741 cacttgtaag gttagcacga gtaagtagct ttgtctttgt tattcctccg ttagcagact
     1801 ccagaataat agccatgata tcaggtctgc tacggttttt cacataacaa tgagatatct
     1861 ggacgatata aatgataagt gaagcaactt agcagacaag atgatagaat aaatttgatt
     1921 actaaattat acctacaaat gcaaattcgt tagaaagaca cttggcttaa aaaaggcagt
     1981 gtgggaagaa atgaagcaac ataacttggc ggatgaggca atcgcctcac ctaagctgta
     2041 aatgcagaag aagaagaaga agaagaagaa gaaccagcta aagtacttcg acaaatcgat
     2101 agttgtcgtt attgataatg gaatcgaaat aagcgataaa gatctattac aggtcaacgc
     2161 tacaattatt acatgtggtc ctatttttct tacccttgca gcttcatttt taagctcatt
     2221 tgacagaaag tttagttcaa cagccctatt cataaaagct ggagctaccc tttcattcat
     2281 tatactctct tttttctcat agatgacttc aatggtgaag ctcgtagact caataaccga
     2341 ggcttctgac acattatggc ttgttgtttt tatttgctgc aatactttac ttttattcga
     2401 tggtctctac acatcagttg aacagttacc agactcttga cagaatgtct gatggtggaa
     2461 ccattccacc attgtgagaa aattgtaagt caattgttat ggttggaagg acgtaggatc
     2521 caagattgct cctctcagtc gactcatcgg cttttttatg tgttgataac gttccagaac
     2581 cgagtattat ttatggtacc ctttagccat tagaaatcat aatcggattg ggatggcgg
//
LOCUS       DASQTF010000137         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_679418,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000137 DASQTF010000000
VERSION     DASQTF010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679418"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VE308_03285"
     CDS             complement(<1..354)
                     /locus_tag="VE308_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZB00029.1"
                     /translation="MKTKEPLEIKDVNIPKPKSDQVLVKVESAGVCHSDLHLWEGGYA
                     GPQGVFMKVEDRGVKFPLTPGHEVAGTIDEIGESVTGFSKGDKVLVYPWIGEGTCPAC
                     RVAEENICDSPRSLGV"
     gene            449..847
                     /locus_tag="VE308_03290"
     CDS             449..847
                     /locus_tag="VE308_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00030.1"
                     /translation="MPFTPVVIVLVTISIVGTIAIPFGDPQFLDRAIALELSFVTLGV
                     LILLDGRLTLNKVPLAKLAMYACIPLAIIIIVGNSLASPHVEMMTTFSKPLNAVMLII
                     GGYLLQAALIGTTILQLVRTRQIIVQKQKN"
     gene            complement(844..2232)
                     /locus_tag="VE308_03295"
     CDS             complement(844..2232)
                     /locus_tag="VE308_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HZB00031.1"
                     /translation="MSFRIDKDSLGEVKVPSSAYYGPFTARAINQYKVTGQKAHINLI
                     RAYVMIKRSAALANNDLKSLDNDKAQAIITACDEILAGKLIDEFVVEAINSGAGTAFN
                     MNTNEVIANRALEILGKTKGEYETINPNDHTNMSQSSNDTFPTAMHVAILLNMMETIA
                     SLDKLIASLERKAREFAGLIKIGRTHLMDAIPVTLGAEFEQYAFSLKQARVKLIHAMD
                     DLHYIGLGGTAVGTGANTPKDYTQHAIQYLSKVSGLDLKSSKNTFYSLQSKYDVANCS
                     SALRNLAIDLNKMANDIRLMASGPTAGFAEILIPAVHAGSSIMPGKVNPSLAECLNMI
                     CFNIIGNDVSVAMAAQAGQFELNVMLPGMLKCVLDSTDMLKNFLPIFAANLVDGIEAN
                     KDRLESYIEKSPVLVTLLNPYIGYLRAAEIYKESLKTNKSIRELVLSKGLMTKEQLDK
                     VLSKENIFGAPV"
     gene            complement(2294..2692)
                     /locus_tag="VE308_03300"
     CDS             complement(2294..2692)
                     /locus_tag="VE308_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00032.1"
                     /translation="MVGSEATSYILRIQRDELERQLYSQRILYVSIKRDWLRNTKLLF
                     MRKAVFIGSGIIEGFVSLDELREDEKKICLENNCYGKIEFAKLVRFYPTVAVEDTPVA
                     GQNTIGLHGASLLRSDALQIERLARIRLII"
BASE COUNT          914 a          748 c          676 g          984 t
ORIGIN      
        1 tactccaagg cttcttgggc tatcacagat gttttcttcg gcgacacgac atgcgggaca
       61 agtgccctcg cctatccagg gatagactag cactttatcg cctttggaaa aacctgtgac
      121 tgattcgcct atttcatcta ttgtcccagc aacttcgtgg ccgggcgtaa gagggaattt
      181 tactccacgg tcttcaacct tcataaatac gccctgtggg ccagcatatc caccttccca
      241 cagatgtaga tcactatggc acacacctgc actttcaacc tttacaagaa cctgatcgct
      301 ctttggtttt ggaatgttga catccttgat ttcaagcggc tctttcgtct tcattatccg
      361 tgcagccttc atatcttgag aggatactaa agatcgttat taatagttag caaaagcctt
      421 taattgaaat ttcggcaaag aaattgtgat gccctttact cctgttgtca ttgtgcttgt
      481 gacaataagc atagtgggca caatagcaat cccctttggc gatccacaat ttctcgacag
      541 ggctatagca cttgagctat cgttcgttac gctaggtgtt cttattcttt tggatggcag
      601 actgactctt aataaagtac cgcttgcaaa attagcaatg tatgcttgca tcccgcttgc
      661 aatcattata atagtgggaa actcgcttgc atcgcctcat gtcgagatga tgacaacatt
      721 ttcaaaacca ctcaatgctg taatgctaat tattggtggt tatctcttgc aggctgcgct
      781 tattggcaca accattttgc agcttgtaag aacgcgccaa ataattgttc aaaagcaaaa
      841 gaactagact ggagcaccaa atatgttttc ctttgagagc accttgtcga gctgctcctt
      901 tgtcatcagt cctttgctca gaaccagctc gcgaatactc ttgttggtct tgagcgactc
      961 tttgtatatt tcggctgccc ttagataacc aatataggga ttcagcagtg tgactaacac
     1021 tgggcttttc tctatataag attccagtcg gtccttgttt gcttcaatgc catccaccaa
     1081 attggctgca aatatcggca agaagttttt caacatgtca gttgaatcaa ggacacattt
     1141 caacatgccc ggtagcataa cattcaactc aaactgacct gcttgtgctg ccatcgcaac
     1201 tgaaacgtca tttcctataa tattaaagca tatcatgttg agacattccg ctaaggacgg
     1261 atttacctta cccggcatta tcgacgaacc agcatgtact gcaggaatca gaatttctgc
     1321 aaagccagca gtcggccccg aggccataag tcgaatatca tttgccatct tgttgagatc
     1381 tattgcaagg ttgcgtaggg ccgacgaaca atttgccaca tcgtacttgc tctggagaga
     1441 gtagaatgtg tttttcgagg acttcaagtc cagccctgaa acctttgata ggtactggat
     1501 ggcatgctgg gtgtagtctt ttggagtgtt ggcaccagtg cccactgctg tgccgccaag
     1561 tccgatataa tgtaggtcat ccattgcatg aataagtttt acccttgcct gcttgagcga
     1621 aaaagcatac tgctcaaact cggcgccgag ggtaacagga attgcgtcca tcaggtgggt
     1681 gcggccaatc ttgatgagcc cggcgaattc tctcgctttc ctttccaagg aagcaatcag
     1741 tttatcaagc gaggcaatcg tctccatcat attcagcaaa atggcaacat gcatcgctgt
     1801 tggaaaagta tcattgcttg actgcgacat gttagtgtgg tcgtttggat tgattgtttc
     1861 atattcaccc ttagtcttgc caaggatttc taatgcccta tttgcgatga cttcattagt
     1921 gttcatgtta aatgcagttc cagcaccgga gttaattgcc tctacgacaa attcatcgat
     1981 tagcttgcct gccaatattt catcacacgc cgttattatc gcctgtgcct tgtcgttgtc
     2041 aagcgatttg aggtcgttgt ttgctagcgc ggccgaccgc ttgatcatga cataagctct
     2101 tatgagattg atatgggcct tttggcccgt caccttatac tggttgattg cgcgagctgt
     2161 aaaagggcca tagtaggcgc tagatggaac tttgacttcg ccaagagagt ctttgtcaat
     2221 cctgaaggac attgccaaat gtgaaaaata ctaaaaaatc ggctataata aattaatcga
     2281 caagaacctt tccctatatt attagcctta tccttgcaag ccgctcaatt tgaagagcat
     2341 cggagcgcag cagactggca ccgtgcagcc caatggtgtt ttggcctgca acaggtgtat
     2401 cttccactgc tactgtagga taaaaccgca ccagtttggc aaactcaatt ttgccgtaac
     2461 agttgttttc aagacatatc tttttctcat cttctcttag ctcgtcaagt gagacaaacc
     2521 cttcaattat gccagaacca atgaatacag cctttctcat aaacagcaac tttgtattgc
     2581 gtagccagtc gcgttttatg ctgacataga gtatgcgttg actatagagc tgtctttcaa
     2641 gctcatctct ttgtattcga aggatgtaac ttgttgcttc gctgcctacc ataattaaaa
     2701 gcacacttta accagtactt caacgtcata gttttaagaa ttcgccaact atttgttttg
     2761 aaagtatgat aatgtgatgc ctttctgact cactagttgc ttttatctaa cagacaacct
     2821 ggggcaaaaa tattagcaag tttgcaaaac tcgatgggca ataacggcaa aaggcctgtt
     2881 cgaaagtaag tatgttgtca gtgatctgta taacctctac agacttccat ctcggaattt
     2941 agaataaccg atgatatcgt accgaaacag gttgatgaat aaacctgttt tttcataaag
     3001 gataacaatg gaagtatata cccggagaac atgaaggtga ttctgcagaa aaagctctta
     3061 cataccgtta gctcatttca ttagcatcag aatttatccc aaaagaacag cagcataatc
     3121 catggttctg aaaaagacct aaaataagag ataatgatag acaagagctt atcgtttatg
     3181 taagaaaatg cctaatttca atgtacatac tatcttccta tcatataggc acgtccaatc
     3241 ccaacaagaa ttaagcaaat tctgcgtcag gaaattaata tcgcaatgat tgatagctca
     3301 agagggaatc acttcatgag aa
//
LOCUS       DASQTF010000138         4054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_775924,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000138 DASQTF010000000
VERSION     DASQTF010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775924"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VE308_03305"
     CDS             <1..797
                     /locus_tag="VE308_03305"
                     /inference="COORDINATES: protein motif:HMM:NF021625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HZB00033.1"
                     /translation="VTDLLLDSKGDINKFTNPRLEIKGIHGSGCNFSSAVTAYLAKGM
                     ALTEACKMANEYVHTAIRNALTIGRGLPIANPLFAIYRDASRYHTLAELQQAAEQVSM
                     LDGFYRLIPETQTNFAYALLNAVDISDVAAVRGRIIKIENTAALASYIKFGASSHVAS
                     AVIAYMSVNPDFRSAINIRFDERIVDVCKSLFSVASYDRTKEPKKSKRREGSSVAWGI
                     LAALSKNPQADVIYHTGDIGKEPMISLFGRNPTEVVNKIKAILKNY"
     gene            856..1563
                     /locus_tag="VE308_03310"
     CDS             856..1563
                     /locus_tag="VE308_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZB00034.1"
                     /translation="MKAVILAAGLGTRLQPYTFFIPKPMLPLGNRPLLEYIIEWLKNS
                     DDIEHIVICVSYLHRTIEDYFEDGKRFGVQIEYARSERPLATAGQLKTAEKLLEDDSF
                     VCVYGDSVYEFNLRKMIRQHKESKAFVTMALLQYSTKLKYGFIDINGHDRVTAWREKP
                     EISGLINIGSYIMEPQFLKIIPKSGSFGMDDAVKKVLEQRRTVKGFRIDTGFIDIGDK
                     KSYLDAYKQYVNKLGKI"
     gene            1564..2832
                     /locus_tag="VE308_03315"
     CDS             1564..2832
                     /locus_tag="VE308_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HZB00035.1"
                     /translation="MSIALFEDSHWQNFAPISLTKATFDVKVGAKSFFEEHQLTPEIL
                     LTREYLACVTAQRHPNCKVNPSSIASDTVFVNGLLHPGAIYPDRLFSINHTFAITART
                     SRRLLVARLDSKDIEYLQDCVTAGKRINVKKLKVEKLTNLEDQDTHGIISEPWDVIRV
                     LENSLATQVSTMKNETQLGEEVTVIGSNTVAVGRDASVEVGTVLDVRNGGIYIGPEAY
                     VAASRIVGPAYIDGMAQVKQFTIIEGSYIGYNCRIGGEVEHSVISDHTNKAHAGFIGH
                     SYIGEWVNIGAMTTTSDLKMTYGNIKMYSGNNSKIDTGQNKIGSFFADMVKTSIGTLI
                     YSGRRIGVSSHLHGLVAQDVPSFTIYGTSIGARNVELKLDSIIETQRKMMRRRNQTMT
                     KAYEQMIKDVFAMTASDRKQIGVRRGKFAI"
     gene            complement(2835..>4054)
                     /locus_tag="VE308_03320"
     CDS             complement(2835..>4054)
                     /locus_tag="VE308_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HZB00036.1"
                     /translation="FGIEEFNLQKFVGCNLQIRGDLMEKASELIVKFGSFGRYCKKHF
                     GTESVLPIVHGDTLVAGIAPLAWVFGMGQKVGQNEAGLRSMCPQAINSLKAGREPTRQ
                     EIQKFVSSQFDGKWYIAREEPFPEQIDTWICSAGTQFFFAPTKLNKDNLVREGYPENF
                     VFVVGNSVVDAIHIKRKEKPQQSIFTFYPQLERDDWIRMDIHRRENLTEKRFMSIIEG
                     LVQLVKNTDKKVALVLLNATIAALKQYSLEFKLHSLAEQYPEKFVMTPLWKEYAHVIE
                     FLDSGHCWAEMTDSGSMQEELLYFPKVTSMTVRLNTDRPETIFDAQSNILVPPLNAGW
                     IVSMVKEAYNREEGLGLKLRHKKQIYGTPGDVSKNIIRIVKKEFENGDANFYPWLHQR
                     LGFWRESQGLNYM"
BASE COUNT         1164 a          875 c          930 g         1085 t
ORIGIN      
        1 atgtgaccga cctgctactc gacagtaaag gcgacatcaa caaatttaca aaccctcggc
       61 tagaaataaa ggggattcat ggttctggct gcaacttttc ctcagcagtc acagcatatc
      121 ttgcaaaagg catggcgctt acagaggcgt gcaagatggc aaatgaatat gtccatactg
      181 ccatcagaaa cgcattaaca atagggaggg ggttgcctat cgcaaacccg ctttttgcca
      241 tttatcgtga tgcaagtcgc taccatacct tggcggaact acaacaggca gcagagcagg
      301 tcagtatgct tgatggcttt tataggctca tacctgagac acagactaat tttgcttacg
      361 ctctcttgaa tgcagtcgat atatcagatg tcgcagcggt gagaggaaga ataatcaaga
      421 ttgagaatac cgccgctctt gcttcttaca tcaagtttgg tgcctccagc catgtcgcgt
      481 cggctgttat tgcatatatg agtgtcaacc ctgacttcag gtctgcaatc aacatcagat
      541 ttgatgaaag gattgttgat gtgtgcaagt cactattctc tgtggcaagc tatgatagga
      601 ccaaagaacc gaaaaagtcc aaaagaaggg aaggatctag tgtggcgtgg ggaatccttg
      661 ctgcgctttc aaagaatccc caggctgatg tcatttatca cacaggcgat attggaaaag
      721 agcccatgat aagcctgttt ggaagaaatc ctaccgaagt agtaaacaag atcaaagcaa
      781 tattgaaaaa ttattgagtc tgtatagttc aatttatatt atatatctca gaagttctga
      841 aaaaagacac agagcatgaa agcagtaatt ctggcagctg gactgggtac aaggctccag
      901 ccttatacgt tcttcattcc aaagccaatg ctccctcttg gcaataggcc tcttcttgaa
      961 tatatcatag aatggttgaa gaactctgat gatatcgaac acatcgttat atgcgttagt
     1021 tatcttcatc gaacaataga agattatttt gaggacggca aacggtttgg agtccaaatt
     1081 gagtacgcaa ggtcggagcg tccgcttgca acagcaggcc agcttaagac tgccgagaag
     1141 ttacttgaag atgactcgtt tgtatgtgtc tatggagatt ctgtctatga gttcaatttg
     1201 cgcaagatga taaggcagca taaagagtca aaggcgtttg tcactatggc gcttctacag
     1261 tactccacta aattaaagta tggatttata gacattaatg gtcacgacag agtcaccgca
     1321 tggcgtgaaa agccggagat aagcggccta ataaacatcg gctcttacat tatggagccg
     1381 cagtttttga agataattcc caagtccgga tcgtttggaa tggatgatgc agtaaagaaa
     1441 gtactggagc aaaggaggac cgtcaaagga tttaggatcg atactggctt tatcgacatt
     1501 ggcgacaaaa agtcatactt ggatgcatac aagcagtatg tgaacaagct gggcaaaatt
     1561 tagatgagca tagccctgtt tgaggactca cattggcaga actttgcgcc aatttcactt
     1621 acaaaagcta catttgatgt caaggtcggg gcaaagagct tttttgaaga gcaccagcta
     1681 acaccagaga tattgcttac aagagagtat cttgcatgtg tcacagccca gcggcaccct
     1741 aactgcaagg ttaacccaag ctccatagct agcgatactg tgtttgtcaa tgggctcctc
     1801 catcctgggg caatctaccc tgaccgcttg ttcagcataa atcacacttt tgccataact
     1861 gccagaacga gcaggaggct gcttgttgca agactcgaca gcaaagacat agaatacctg
     1921 caggattgcg ttacagcagg caagaggata aatgttaaga aattaaaagt ggaaaaattg
     1981 accaatttgg aagatcaaga cactcacgga atcatctcag agccttggga tgtaatcaga
     2041 gttcttgaaa attcccttgc cacacaggtt tctactatga aaaatgaaac gcagctaggc
     2101 gaagaagtca cagttatcgg cagtaacact gttgcagttg ggagagatgc ctcagtagag
     2161 gttgggactg tgctagatgt aagaaatgga ggtatataca taggcccgga agcttatgtt
     2221 gcggcaagca ggatagtagg tccagcatat attgatggta tggcgcaggt taagcagttc
     2281 actatcattg aagggagcta tatcggctac aattgcagaa ttggtggcga agtagaacac
     2341 tctgtaattt ctgatcacac aaacaaagct catgcaggat tcattggcca ttcatatatt
     2401 ggtgaatggg tcaacattgg cgccatgact accacatcgg accttaagat gacatacgga
     2461 aacatcaaga tgtacagcgg caacaacagt aagattgaca ctggtcaaaa caagataggt
     2521 tcgttttttg cagacatggt caagacatct attggcacgc ttatttacag cggcagacgg
     2581 atcggcgttt cttctcactt gcacggcctt gttgcacaag acgtgccaag ctttactatt
     2641 tatggcacaa gcataggagc gagaaatgtc gagctgaagc ttgattcgat aatagaaact
     2701 cagaggaaaa tgatgaggcg acgtaaccag acgatgacca aagcatatga acaaatgata
     2761 aaggatgtat ttgcaatgac cgcatcggat agaaagcaga tcggtgtaag gagaggcaag
     2821 tttgccatct aatgctacat gtagttaagt ccttgacttt ctctccagaa ccccaatctc
     2881 tggtgcagcc acgggtaaaa gttggcgtcg ccattttcaa attccttctt gactattcgg
     2941 atgatattct ttgagacatc tcctggcgtg ccgtatatct gttttttatg tcgcagcttc
     3001 aatcccaaac cctcttctct attgtaagcc tctttaacca tagagactat ccaaccggca
     3061 ttcaatggag gaacaaggat gttgctctgg gcatcaaata tcgtttctgg cctgtcagta
     3121 ttgagcctga ctgtcataga tgttaccttg ggaaagtaga gcagctcctc ctgcattgag
     3181 ccagaatccg tcatttcagc ccagcaatgt ccgctgtcaa ggaattctat tacatgtgcg
     3241 tattctttcc atagaggcgt catcacaaat ttttctggat attgttctgc aaggctatgc
     3301 agtttgaact caagactgta ttgcttgagt gcagctatgg tggcgtttaa caacactagg
     3361 gcaactttct tgtctgtgtt cttgacaagt tgtacaagtc cctcaataat tgacataaag
     3421 cgcttctctg tcaagttttc tcgcctatga atgtccattc ttatccagtc gtccctttca
     3481 agctgagggt aaaaagtaaa aatgctctgc tggggctttt cttttctctt aatgtggatt
     3541 gcatccacca ccgagttgcc gacaacgaag acaaagttct ctggatatcc ttcccttact
     3601 agattatctt tgttaagctt cgttggagca aagaagaatt gtgtgcctgc tgagcatatc
     3661 cacgtgtcaa tctgctctgg aaagggttct tccctagcaa tataccattt gccgtcaaat
     3721 tggctgctaa caaatttttg tatttcctgt cttgtcggct ctcgtcctgc ctttagcgaa
     3781 tttatggctt gggggcacat ggaacgaagg cccgcctcat tctgcccaac cttctggccc
     3841 atgccaaaaa cccatgcaag cggggcaatg cctgccacca gtgtatctcc atgcactatg
     3901 gggaggacag actcagtccc aaagtgcttc ttgcagtacc tcccaaacga gccgaacttg
     3961 actattagct cgcttgcctt ttccattaag tctccacgta tctggaggtt gcagccaacg
     4021 aacttctgca gattgaactc ttctataccg aagg
//
LOCUS       DASQTF010000139         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_680243,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000139 DASQTF010000000
VERSION     DASQTF010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680243"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..460
                     /locus_tag="VE308_03325"
     CDS             50..460
                     /locus_tag="VE308_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HZB00037.1"
                     /translation="MSEQELPPWVREQVSRLQQLQQNLQGIMMQKQQLEVEMVETDRG
                     LQELKKASPDDAIYKNAGSVLIKAKKEDVLKELEEKKELSNTRIMVLGKQEARVKENL
                     KEVENKINEMIRGMQAGGSSTAGSSGSAGPRSLG"
     gene            674..1402
                     /locus_tag="VE308_03330"
     CDS             674..1402
                     /locus_tag="VE308_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HZB00038.1"
                     /translation="MPVRIVVDEREKNSNIPQLLKNAGAVIDFTQLKVGDYVVSSETA
                     VERKTVRDLISSIYDGRLFVQCSDLVRYYQKPLLVVQGNIAELAETSEDMEDSDIKPH
                     AEQMPIAYDALITVATEFRIPIIHTPSAEQTAQLLVTLVNRSLREGKATGPLLRKIKK
                     ENPIYIQQLSVLASLPGVGEKLAVRTLKKFHTPIRALNASAAELATIPGFGLARAERV
                     RKVLNISNNVKQVMQRTLFDDQEK"
     gene            complement(1635..2138)
                     /locus_tag="VE308_03335"
     CDS             complement(1635..2138)
                     /locus_tag="VE308_03335"
                     /inference="COORDINATES: protein motif:HMM:NF028455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZB00039.1"
                     /translation="MDSQDSAFKYALDAGAFYTGFVFLSSGYRHCTTQAVFDEVKHIK
                     RSHGAIEALLESNNLQILNLDTKSIKKVVAASRRTGDYTKLSEADISIIALALQLEIV
                     LVTDDFAVANVATTLKIPVKSVASKGITHTRSWIAYCSACSRAFGPNAKECRLCGNRL
                     RRKYKVI"
     gene            complement(2113..2949)
                     /locus_tag="VE308_03340"
     CDS             complement(2113..2949)
                     /locus_tag="VE308_03340"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZB00040.1"
                     /translation="MAQNIRNVAIITKTGSNEADSAAIKVATLLNRRKIKVHTVLPFI
                     MEDATPITPDKELKDIKLDLVFSIGGDGTTLRAFRTVPGKVPLFSINSGGHRGILSEI
                     DTESIDEALKTILAGKYFYDRRIRIQASVNGTLFPPVLNDILIIRVNLTRTPLLSVML
                     MGDEIKQRMDGIVVSTPTGSTGHSFSVGGPVLHEGMSCLSLTPIASVNRMPQLVIPPE
                     EILIRSTHESHMILDGQETFKIHADEPINILRCSDDAIFLRLQKKGMRQLAKLGFSRF
                     GF"
     gene            3039..3896
                     /locus_tag="VE308_03345"
     CDS             3039..3896
                     /locus_tag="VE308_03345"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZB00041.1"
                     /translation="MTVSGYAYPEVLCETEWVAENLNNPNIRILEVDYDPENAYKEGH
                     VPGAHLVWWKRDINDPIRRDIMDKQQFEGLMSRIGATHDTELILYGDFNNWFAAFAFW
                     VFQYYGHKKIKIMNGGRKKWELERREYSKDDPITSPTKYVAKPPEEGIRAYMPDIRRA
                     IEKAEQTVLVDVRSPKEFSGEITAPPEYPTEHAQRGGHIPGAKNIPWGQAVRETDGTF
                     KSREELEALYEGKGVTPDKPVICYCRIGERSSHSWFVLKYLLGYPSVRNYDGSWTEWG
                     NMIGNPIEK"
     gene            3921..4226
                     /locus_tag="VE308_03350"
     CDS             3921..4226
                     /locus_tag="VE308_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00042.1"
                     /translation="MQEQGNLIKKGQFYFIYDHNLNLVLEDKTKRGLEVRENIFDEKL
                     GVQADKGTIYDLDGIGHKVGIRWYFPKSKYRLDHVIKIGNEMELRYKAIREMTCPDD"
BASE COUNT         1310 a          955 c         1033 g         1122 t
ORIGIN      
        1 cctaacgcta tgattttata tgctgccgat ctcagtagct ctgcagttta tgagtgaaca
       61 ggaacttccc ccatgggtaa gagaacaggt atcgcgatta cagcagctcc aacagaacct
      121 gcagggaatc atgatgcaaa aacaacagct agaggttgag atggtagaga cagacagagg
      181 tcttcaggaa ttgaaaaaag ccagccctga tgatgcaatc tacaagaacg caggttctgt
      241 actcatcaag gccaagaagg aagacgtcct caaggagcta gaagaaaaga aagaactatc
      301 aaatacacgt attatggttc ttggcaagca ggaggcaaga gtaaaagaaa atctaaagga
      361 agttgaaaac aagataaacg agatgatccg aggtatgcag gctggtggat cttcgacggc
      421 tggatcaagc ggcagtgccg gcccaaggtc attgggatag tggtaccgct tactactgcg
      481 tctagtacta tggaattttc aacagcctat tttgttatat gatggagcac ttgccacgaa
      541 aattttagaa ctagccacaa cgtggagatg tatagcgagt gactatgcag agagagagcc
      601 ttacatattg ttgcagttga tttgctcatg tcgtgaaata tagttataga gggcctaaaa
      661 cagagtatgt caggtgcctg ttagaattgt agttgacgag agggaaaaga acagcaatat
      721 acctcagcta ctgaaaaatg caggcgctgt cattgacttt acacaattaa aagtaggtga
      781 ctatgttgtt tcatctgaaa cagccgtaga gcggaaaacc gtgcgtgacc taataagctc
      841 aatctacgat ggtcggctct ttgtacagtg ctctgattta gtgaggtact atcaaaaacc
      901 actattggtt gtgcaaggca acattgccga gcttgctgaa acttctgagg atatggaaga
      961 ctctgatatt aagccgcatg ccgagcaaat gccgattgca tacgatgccc tcataacagt
     1021 cgcaacagag ttcagaatcc caattattca taccccttcc gcagaacaga cagcgcagtt
     1081 gctagtcaca ttagtcaacc ggagcctaag agaaggcaag gccacaggtc cactgttaag
     1141 aaaaattaag aaggaaaatc caatttacat acagcaactt tccgtgcttg catctttacc
     1201 cggagtagga gaaaaacttg ccgtcagaac gcttaagaaa ttccatactc cgattagggc
     1261 acttaacgcc tctgcggcag agctcgccac gataccaggc tttggtcttg caagggctga
     1321 gcgagtgcgt aaggtcctca atatatccaa taatgtcaaa caagtaatgc agaggactct
     1381 ttttgacgat caagaaaaat aaaagctcaa ttcaaaacat cggacaataa aggatgcagt
     1441 tgtctgttag tggcttgcat cagatgttta taatcggtat cttatattat gaagctatac
     1501 tgaagtatgc cattaatcgg cgtacgctgg caaggacgca gcgcagtaaa ggctaactca
     1561 agtcaaaaaa agctcttacc ccaagctcgg ctatatcgca agtcgataat cccccgaaaa
     1621 gaagtgcagt tcgctcagat aactttgtat tttcgcctta acctgttgcc acacaaccta
     1681 cactcttttg cattaggtcc aaacgccctg ctacatgcgc tgcaataggc gatccaactt
     1741 cttgtatgcg taataccttt actggccaca gacttaacgg gaatcttcaa agtggttgca
     1801 acatttgcaa ctgcgaagtc gtcagttacc aaaacaattt ccagctgcag cgcaagggca
     1861 attattgata tatctgcctc tgaaagcttt gtatagtctc cggtcctcct tgatgcggca
     1921 acaacttttt tgatgctttt tgtgtctaaa tttagtatct gcaagttatt ggattcaagc
     1981 aacgcttcta tcgccccgtg tgaccttttg atatgcttta cctcatcaaa aacggcctgt
     2041 gtagtgcagt gtcggtaccc tgatgacaga aatacaaagc ctgtgtaaaa tgcacctgca
     2101 tcaagagcat atttaaaagc cgaatcttga gaatccaagc ttcgccagct gcctcattcc
     2161 ttttttctga agccgtaaga atatggcatc gtcagaacaa cgcaaaatgt tgatgggctc
     2221 atctgcatga atcttgaacg tttcttgacc gtcaagtatc atgtgagatt cgtgcgtgct
     2281 ccgtataagg atttcttccg gtgggatcac taactgtggc attctgttca cagatgcaat
     2341 cggagtcaga ctcaggcagc tcatgccttc gtgcaagact ggccctccaa ccgaaaaaga
     2401 atgacccgtt gatcccgtag gcgtagagac tactatgcca tccatcctct gctttatttc
     2461 gtcacccatc aacataacgg ataatagcgg agttcttgtg aggttcactc ttataatgag
     2521 tatatcgttt aggacaggcg gaaaaagcgt gccgttgaca gaagcctgga tccttatgcg
     2581 acgatcataa aaatacttgc ctgcaagtat tgtcttgagt gcttcgtcta ttgattctgt
     2641 gtcgatttca gacaggatgc cgcgatgacc tccactattt atgctaaaga gcggcacctt
     2701 tcctggtaca gtacgaaaag ctcgcagcgt cgtgccgtct ccacctattg aaaagacaag
     2761 gtcaagctta atgtccttca attctttatc aggcgtgatt ggcgttgcat cttccataat
     2821 gaagggaagc acagtgtgga ccttgatttt ccttctgttg agcagagttg caacttttat
     2881 agctgcactg tcggcttcat tactccctgt cttagtgatt atcgctacat tgcggatgtt
     2941 ctgtgccatc tcgccgcact gctatagcag cagacatgct attaaatccc tttacctctg
     3001 acaagaatta taagtagcgc ctgaaggtag ccatcattat gacggtgagt ggatatgctt
     3061 atcccgaagt tctatgtgag accgaatggg ttgcggagaa ccttaataac ccaaacatca
     3121 gaattttgga agttgactat gatccagaaa atgcatacaa ggagggacac gtccctggcg
     3181 cacatcttgt atggtggaag cgcgacatta atgatcctat ccggcgcgat atcatggaca
     3241 agcagcagtt cgagggcctt atgagcagga ttggtgcgac acatgacacc gagctcattt
     3301 tgtatggtga ttttaataat tggtttgccg cttttgcatt ctgggtcttt cagtactatg
     3361 gccacaaaaa aatcaagata atgaacggtg gccgaaaaaa gtgggagctt gaaaggcgcg
     3421 agtactcaaa ggatgatcca ataacttcgc cgacaaaata cgttgcaaaa ccaccagaag
     3481 aaggcatacg ggcttacatg cctgatataa gaagggcgat cgaaaaagca gagcagacgg
     3541 tgcttgtaga tgtacgctcg ccaaaagagt tttccggtga aatcactgcg cccccagagt
     3601 acccgacaga acatgctcag cgaggtggcc atatacccgg tgcaaagaat atcccctggg
     3661 gccaggcggt cagggaaaca gatggcacgt tcaagagccg agaagagcta gaggccctct
     3721 atgaaggtaa gggcgtaacc ccggataagc ctgtcatctg ctactgcaga atcggagagc
     3781 gctcatcaca tagctggttc gtactcaaat atctgcttgg ctatccatct gttcgtaact
     3841 atgatggctc gtggaccgaa tggggcaaca tgatcggaaa tccgatagag aaataagtcc
     3901 ttttcttcct tctgtcatcg atgcaagagc aaggaaacct gatcaagaaa ggccagtttt
     3961 acttcattta tgaccataat ctcaatcttg tactcgaaga caagacgaag cgtggtttgg
     4021 aagtgcgaga gaatatcttt gacgaaaagc ttggcgtgca agcagacaaa ggaacgatat
     4081 atgacttaga cggcataggc cataaggttg ggattcgatg gtatttcccg aagtcaaaat
     4141 ataggttaga tcatgtcata aagattggaa atgaaatgga attgcggtac aaggccatac
     4201 gggaaatgac atgtccggat gattaatatt agatcgaaat acttttagga gaactaaata
     4261 ctagataatt ccagatatca tgtctgtctt attgagggat agggtctgga cgatgctatc
     4321 tgaagtcgcc aagaggggcg tttatccact gcacggcaac aagctgggca tatacaggat
     4381 cccactcgag attactcaaa gctcagaaat acaaagtatc
//
LOCUS       DASQTF010000140         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_594727,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000140 DASQTF010000000
VERSION     DASQTF010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594727"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1242
                     /locus_tag="VE308_03355"
     CDS             175..1242
                     /locus_tag="VE308_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00043.1"
                     /translation="MVNTGPVVTLSECGLVVHISGRKILLDPRRESTSDVMFFSHAHS
                     DHLMTSKKKKIGHAEQVLASKATSHLARQRGVDLSLANYKDCCDGYRLIDSCHVLGSR
                     GLLIDDQIYYTGDISTRKRGFMGGAQVPKADVLIVESTFGSPRYVFPRIDDVIHTANK
                     IIGEMFDRGVSVLLLGYPLGKAQLLTQLFQHWAPLFIYDSVAKINTIYREFGVGLKEG
                     ITFSTAKQGSLLAKSTPWLMVAPLSSARSGFIRAIKERCRAISIGFSGWAIDKSYKFM
                     MGLDYALPLSDHCDYEELVQLVLLSRARKIYTFHGFSTEFARSLKGLGLDAEPVARAH
                     KPQPSKASTQKSGTLDAYLQA"
     gene            complement(1239..2321)
                     /locus_tag="VE308_03360"
     CDS             complement(1239..2321)
                     /locus_tag="VE308_03360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZB00044.1"
                     /translation="MNVKFGFTQGLNVARLGLDESQVITACQIADRMDYDSVWSMDHS
                     NVPQWKNAVVNDAWLMLAAIGAVTSHVELGTCVTDAIRRHPSAIALSTITLDRITKGR
                     AILGIGAGEAQNVVDFGIEFSKPVSKFKEQLEVIEKLFESEPDRRVSYKGQHYNLVEA
                     CLQARSIRKPRPPVYIAAGAPKTLELCAKYGDGWIPIGYTPELFKNHANVIRDRAKEI
                     GRDLNGFQFANDVDIYFTDDGEEAWNKMKNAVKVSLYKPELLKVHNIQQDSEFDFRRY
                     FTEYAMNKPELMEQMKRAALKIPDSVARTAIGVGKPEDVIQMLERFIDAGTNHFIIRF
                     WGDGYFKNIETFGNKVITYFRDQGRA"
BASE COUNT          639 a          567 c          514 g          689 t
ORIGIN      
        1 tgcattggta ccaattaggg ttcgagttgc aattgcctga agtattgagc gcaaattgct
       61 tcccctccct gagatcaaaa ttgctagttt tagcaccctg aaaatatctt agaacgcgct
      121 taaacgttta gcgctgtaaa attaaaataa ctgcaatcac accattagag attattggtc
      181 aatactggtc ctgtggtgac cctatcagag tgtggacttg tcgttcacat tagtggacgc
      241 aaaattctgc tagatcctcg gagggaatcg acatcggatg tgatgttttt ttcccatgcc
      301 cattcagacc atctgatgac atcaaaaaag aaaaaaatag gtcacgctga gcaagtccta
      361 gcatcaaagg cgacgtcaca tcttgcccgg cagcggggcg tcgatttgtc tcttgcaaac
      421 tataaagatt gttgtgatgg ttataggtta attgactcat gccatgtcct tggatcaagg
      481 gggttattga tagatgacca gatctattac acgggggaca tctcgactag gaaacgaggc
      541 ttcatgggag gcgctcaagt gccgaaagca gatgtcctaa tcgtggaatc aacctttggc
      601 tcccccagat atgtcttccc tcgtattgat gacgtgatac acacagccaa taaaataata
      661 ggtgaaatgt tcgacagggg agtatctgtg cttctcttgg gatacccact gggtaaagct
      721 caattgctca cgcagttatt tcagcattgg gctcctctat ttatttacga ttcagtggca
      781 aaaataaata cgatttacag ggaattcgga gttggcctaa aagaaggaat aacattttcc
      841 acggcaaaac agggatctct cctagcaaaa tcgacaccat ggttaatggt cgctccctta
      901 agctcagcca ggtcaggatt cattcgagca attaaggaac gatgccgagc gatttcaata
      961 ggctttagcg gctgggcaat tgataagagc tacaagttca tgatgggttt ggattatgcg
     1021 ctcccgttaa gcgaccactg tgattatgaa gaattggtac agctcgtctt gttgtctcga
     1081 gcgcgtaaaa tttatacttt ccacggattt tctaccgaat tcgctcgatc gctgaagggt
     1141 ctcggccttg atgcggagcc tgtggctcgt gcgcacaagc cccagccgtc caaagcgtca
     1201 acgcaaaaga gtggcacctt ggacgcgtat ctgcaagcct aggctctacc ttgatctcta
     1261 aaataagtaa ttaccttgtt cccgaatgtt tctatgttct taaagtatcc atccccccag
     1321 aaacgaatta tgaaatgatt agttcctgca tctataaacc gctccaacat ttggattaca
     1381 tcttcaggct tgccaacgcc tattgcagtt ctcgcaacgc tatccggtat ctttaatgct
     1441 gccctcttca tctgttccat aagttctggc ttattcatag cgtattcagt aaaatacctc
     1501 ctgaaatcga actcagagtc ttgctgaata ttgtgaacct ttagaagttc tggcttgtat
     1561 aaactcacct ttactgcatt tttcatcttg ttccaagctt cctctccatc gtctgtaaag
     1621 taaatgtcga catcgttagc gaactgaaac ccatttagat cccgtcctat ttctttggct
     1681 cggtctcgta ttacattggc atgattcttg aatagttcgg gagtgtaacc aattggaatc
     1741 cacccgtcac catactttgc gcatagctct aaagtcttcg gagccccggc ggcaatataa
     1801 acaggtggcc taggctttcg aatgctcctg gcttgaaggc aggcctcaac aaggttatag
     1861 tgttgtccct tgtagctcac acgccggtct ggttccgact caaaaagctt ctctattact
     1921 tcgagctgtt ctttgaattt cgagacgggt ttcgagaact caattccaaa gtctacaaca
     1981 ttttgcgcct ccccggcacc gattcccaat atagccctgc ccttggttat tctatccaaa
     2041 gtaatagtcg aaagggcgat agccgaagga tgtctcctta tcgcgtctgt tacacacgtt
     2101 ccgagttcca cgtgagacgt aacggctcct atagctgcta gcattaacca agcatcattg
     2161 acaacagcat ttttccattg aggaacgtta gagtggtcca tcgaccaaac tgaatcgtag
     2221 tccattctat cagctatttg acaggcagtt atcacttggc tttcatctag ccccagtcgg
     2281 gcaacgttga gcccctgtgt aaatccaaac ttgacgttca ttcagaaatg taatctctcc
     2341 tatgttggct aggaataagg agaaaaagaa atatgatctc tcccattagt tcaagaatca
     2401 atactacgt
//
LOCUS       DASQTF010000141         4623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_777415,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000141 DASQTF010000000
VERSION     DASQTF010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777415"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VE308_03365"
     CDS             <1..275
                     /locus_tag="VE308_03365"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HZB00045.1"
                     /translation="ETRFCHAPLFEVYARLRDHTPVIRIEIMSSASEQELKSILNKLR
                     YHSVAGYPYCLKLAHNTCKISNEDIDRLASIYDLQNEQGARDALNE"
     gene            268..1803
                     /locus_tag="VE308_03370"
     CDS             268..1803
                     /locus_tag="VE308_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZB00046.1"
                     /translation="MSEELGILIGVTKPDQVTFESRRPVSIGEYVILNYGGDKVLGLV
                     ERSSISSDALGSSIRNYEEAFESKQVAAENRRDKSYKGHVRILGYVAELKKCKAILPA
                     LPPEPGTEVYEASVKDLAAIFAPSGQQWVMIGTLLRNTAVDARVDIDKVVSRHLAVLA
                     MTGMGKSNLVSLLAKEIARISGTMVIFDYHDDYSTLDMGSNNINLLDGRINPRLLSAD
                     KLAEVVEIQENASNQMHVLRVAFTEEVKQYKGEDFWEALINSASEVGTDKLYREAAAK
                     VVDKIDDARRKFHSILDPSMTDPLALLKNYKINVINLVELTERQANIAVSFYLEELLD
                     DRKKATRQKKGATKSQPRFPAPVLVAIEEAHVFIPKDEDTDTKYFASKVAREGRKFGL
                     GLVIVSQRPRGIDANILSQMGSLAVMRMIQQDDQMHVSAASEALSRDLIDQLTSLNPG
                     EAIFAGQWVNLPTFVKVGEVKERKIGGDQKAVEQWRQLAALKQQAAKESSESYIPSGY
                     IQD"
     gene            1829..2977
                     /locus_tag="VE308_03375"
     CDS             1829..2977
                     /locus_tag="VE308_03375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HZB00047.1"
                     /translation="MLMSHISDLHLGYSQFSLEEREEDVYQAFHEAIDMSIKERVRLV
                     ILAGDLFHNPRPCGKAIITLGNILKKLKEKQISVVFVLGEHDISRLRDVPFVHIYSNL
                     GLAKKLRYDEPFKIENCVLFGADKERHSSIDSLIEKMRHAEQLAKQHDGNRVLVLHQG
                     LADFNKFAGELNSADLPAGFNYYALGHYHDHYEKRFEFLGGPLAYPGSIDLTPSEGIK
                     EVDKGFIITDMSGEEPSTQWVKLESRRQQFSAKIDYKDIENEVEQIIKKATAYEKKPV
                     AKIEVAGSNIDSKVISEHLKKLNQYCLHYIWQPLEEHLATTVYDSKPADIDSELYRLS
                     AEVLGSDELAKFTVGEVLPLAADKDDKSVLEILWQAYKLNKFGGHNAK"
     gene            2974..>4623
                     /locus_tag="VE308_03380"
     CDS             2974..>4623
                     /locus_tag="VE308_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HZB00048.1"
                     /translation="MIKDIQLKNFIAHKDTKIEFCKGITIFLGHNGSGKSSVIDAVTF
                     SLFGKHTRKSNKNLVRRGANHAMAQVRFALNSKEFQAIRALNGSGLQSFSQLVLVSDG
                     DKIVNKPIVGGERRQFGESMSVEIARVLGLNYEKLRVAGVVQQGELVRIVEAQPKDFK
                     ELLNGLIGIDRLDSAYETMRNVIIGFRERLRDEIGYTDEEVPKVERLLEEKEKELKEG
                     SLLLKEFEDERRLLEEKIGQLEKEIERLEPLIQKTQELQNCEKLLVRHVTERRSHLST
                     EVERMERVVMEAKNLLDVMKTKEEVQIRFKMIKAESEEIHSKLEENEGMMGKLLGFLE
                     CAGKLHIADGKCPVCNSPVTKVNEMFNLDHIQLEIRRRSEEKYKLQVVKVELKKEEQQ
                     LAEQDKKIETAEKFLSNNSISSESDLVKIEDDFNSKKKYLAKLPLQIAYVGDDPFKLV
                     IDGVSKTLAEDIATLRAQMSGLSLQQYTQQYTDRKLERTRLIQELQTASVKTGEYRRV
                     ITDAQAAIDSAKHALEQLRQASEFSKMLERIRSMIFNRDGMV"
BASE COUNT         1430 a          951 c         1208 g         1034 t
ORIGIN      
        1 ttgaaacacg gttctgccat gcgccattgt ttgaagtcta tgcaaggctg cgcgaccata
       61 ctcctgttat ccggattgaa atcatgagca gcgcaagtga gcaggaattg aaatcgattt
      121 tgaataagct gaggtaccac agtgtagccg gctatccgta ctgcctgaag ctggcgcaca
      181 atacatgcaa gataagcaat gaagacatag acagactcgc aagcatatat gacttgcaga
      241 atgagcaagg ggcaagggat gctctaaatg agtgaagaac taggcatcct gatcggtgtc
      301 acgaagccag atcaggtgac atttgaatca agaaggcctg tcagcatcgg cgaatacgtt
      361 attctcaact atggggggga caaggtgctt gggcttgttg aaaggtcgtc cattagcagt
      421 gatgcactag gcagcagcat tcgcaactac gaagaggcat ttgaaagtaa acaggttgcc
      481 gccgagaacc gtcgagacaa aagttacaag gggcacgtac gcattcttgg ctatgtggcc
      541 gaattgaaaa agtgcaaggc aatactgcct gcactgcccc ccgagccggg caccgaagta
      601 tacgaagcgt cggttaagga tcttgctgcc atttttgcac cctccggaca gcagtgggta
      661 atgataggta cgcttctccg caatactgca gtggatgctc gagtggacat tgacaaagtc
      721 gtttcgcgtc accttgcagt gcttgccatg actggcatgg gtaagagcaa tctagtctcg
      781 cttttagcta aagaaatcgc cagaataagc ggcacaatgg tgatttttga ctaccacgac
      841 gactattcta cccttgacat ggggtctaac aacatcaacc tactggatgg aaggataaac
      901 ccgcggcttt tgtcggcaga caagctcgca gaggtagttg agattcagga aaacgcttca
      961 aaccagatgc atgtccttag ggtggcgttc acagaagagg tcaagcagta taagggtgag
     1021 gacttttggg aagcgctcat caactctgca tcagaagttg gtacggacaa gttgtatcgc
     1081 gaagcagccg ccaaggtcgt tgataagatc gatgatgcaa ggcgcaagtt tcatagcatc
     1141 ttggatccta gtatgacaga tccattggct ctcctcaaga actacaagat aaacgttatc
     1201 aatctggtag aacttactga gcgacaggct aacattgctg tgtcattcta ccttgaagag
     1261 ctgctggacg ataggaaaaa ggcaacacgc caaaagaagg gcgccaccaa gtcgcagccg
     1321 cgatttccag ctccggtact tgtggcaata gaggaggcgc atgtatttat accaaaggat
     1381 gaagacacag atacaaaata tttcgcttct aaggttgcgc gtgaagggag gaaatttggg
     1441 cttgggctag tcatagtgtc gcagcgccca agaggaatag atgccaacat tttgagtcaa
     1501 atgggctcac ttgcagtgat gcgcatgatc cagcaggacg atcagatgca tgtttctgca
     1561 gcaagcgaag cactcagccg ggatcttatt gatcagctta catcattaaa cccgggcgag
     1621 gcgatatttg cgggtcagtg ggttaaccta ccgacgtttg tcaaggtggg cgaagtcaag
     1681 gagcgaaaga ttggtggcga ccagaaggcg gtagaacaat ggaggcagct ggcagcgttg
     1741 aaacaacagg ccgccaagga atcatcagag tcctacattc catcaggcta tatacaggat
     1801 tagcagttga tgatcctaca gaatacaaat gttgatgtca catatctctg acctccacct
     1861 tggatattcg caattcagct tagaggaaag agaggaagat gtctaccagg cttttcatga
     1921 ggcaatagat atgtcaataa aggagagagt aaggcttgtc attttagccg gcgatctatt
     1981 tcacaatcct cggccatgcg gcaaggcaat aatcacgctt ggcaacatac tgaaaaagct
     2041 caaggaaaag cagatctcag tggtctttgt gctgggcgag catgacatca gccggcttcg
     2101 cgatgtgcca tttgtccaca tttacagcaa ccttgggctt gcaaagaagc tccgatatga
     2161 tgaacctttc aaaattgaaa attgtgtatt gtttggggca gacaaggagc ggcacagtag
     2221 tattgattca ctaatcgaaa agatgcgcca tgcagagcag cttgcaaaac aacacgatgg
     2281 caatagggtg cttgtgctgc atcaggggct ggctgacttt aacaagtttg caggcgagct
     2341 caattctgcc gatcttcctg cagggtttaa ctattacgca ctgggtcact atcatgacca
     2401 ctatgaaaag aggtttgaat tccttggcgg accacttgct tatcctggct caatcgacct
     2461 gactccaagc gaagggatca aggaggtgga caaaggcttt atcataaccg atatgtctgg
     2521 cgaggagcca tcaacacaat gggtaaagct tgagagcagg cgtcagcaat tctctgccaa
     2581 aatagactat aaagacattg aaaatgaggt cgagcagata atcaagaagg caacagccta
     2641 tgagaaaaaa ccagtcgcaa aaatagaggt agctggaagc aacattgatt ctaaggtgat
     2701 atctgaacac ttgaaaaagc tcaatcaata ttgtcttcac tacatttggc agccgctaga
     2761 agagcatctt gctactactg tgtatgattc caagccggca gacattgata gtgagctcta
     2821 tcgtctgtcg gcagaagtac ttgggtcaga cgagcttgcc aaatttacgg taggcgaggt
     2881 attgccacta gcagctgaca aggatgacaa gtcggttctg gaaatactat ggcaggcgta
     2941 caagttaaac aaatttggag gccataatgc taaatgataa aggatatcca gctgaagaat
     3001 ttcattgcac acaaggacac gaagatagaa ttttgcaaag gtataacaat atttctaggg
     3061 cataacggct ctggcaagtc ctcagttatc gacgccgtga cgttttcgct ctttggcaag
     3121 catacaagaa agtcaaacaa gaatcttgta agaagaggcg ccaaccatgc tatggcgcag
     3181 gtgcgctttg ctctcaactc taaagagttc caggctataa gagcgctcaa cggctctggc
     3241 ctgcagtcgt tctcccagct tgtacttgta tcagatggcg acaagatagt aaataagcca
     3301 attgtaggcg gcgagcgcag acaatttggc gagtcaatga gtgtcgaaat agcacgagtg
     3361 ctcgggctca actatgaaaa gttgcgtgtt gcaggggtag tgcagcaggg cgagctggtc
     3421 agaatagtag aagcacaacc aaaagacttt aaagaattgc taaatggcct gattggcatt
     3481 gacagactcg actcagctta tgaaacaatg cgcaatgtta tcataggctt tcgcgaaagg
     3541 ctgcgtgatg aaataggcta cactgacgag gaagttccaa aggtggaaag gctgcttgag
     3601 gaaaaagaga aggagctaaa ggagggatca ttgcttttga aagagtttga agatgaaaga
     3661 aggctgcttg aggaaaaaat aggtcaactt gagaaagaaa tcgaaaggtt ggagccactt
     3721 atccaaaaga cacaggagct tcagaactgc gaaaagctgc tcgtgcgcca tgtcactgaa
     3781 aggcgcagcc atcttagcac agaggtagaa cgaatggaaa gagtagttat ggaagcaaag
     3841 aacttgctcg atgtgatgaa aacaaaagaa gaagtgcaga tacggttcaa gatgataaaa
     3901 gctgaatcag aggagatcca ttcaaagctg gaggaaaatg agggtatgat gggcaagcta
     3961 cttggctttc ttgagtgtgc cggcaaactg catatagcgg acggcaaatg ccccgtttgc
     4021 aactctcccg ttacaaaggt taatgaaatg tttaacctag accatattca gctggagatc
     4081 cggcgcagat cggaagaaaa gtacaaactg caagtagtca aggttgagct caagaaagaa
     4141 gagcaacagc tggcagagca ggacaaaaag atagaaacag cagaaaagtt cctgtcaaac
     4201 aattctataa gctctgaaag tgacttggtc aagattgaag acgacttcaa tagtaagaaa
     4261 aaatatctgg ccaaacttcc gctacaaata gcctacgtgg gagatgatcc tttcaagctg
     4321 gtaattgatg gcgtctcaaa gacacttgct gaagatattg ctacattgcg ggcacagatg
     4381 agtggcttaa gcctccagca atacacccag caatacacag ataggaagct agagaggaca
     4441 aggctgatac aagaattgca gactgcaagt gtcaaaactg gcgagtacag acgtgtaata
     4501 actgacgccc aggcagccat agattctgcc aagcatgcat tggagcaatt aagacaggca
     4561 tctgaattct ccaaaatgct ggaaaggata cgctcgatga tattcaatag ggacgggatg
     4621 gtg
//
LOCUS       DASQTF010000142         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_226328,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000142 DASQTF010000000
VERSION     DASQTF010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226328"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="tgtA"
                     /locus_tag="VE308_03385"
     CDS             complement(<1..1262)
                     /gene="tgtA"
                     /locus_tag="VE308_03385"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HZB00049.1"
                     /translation="MFEVWHSDLAARIGRLQTLHGVVETPAFVPVVHPVRQTVSTQFL
                     KKMGFDLLITNAYITLRHYGDEARRRGIHNIIKYDGAVMTDSGGYQVLEYGSVEVEPA
                     MMAQFENDIKSDIPIPLDRPTGYGLEHKKAKEYVAITLKNALESLRVLGNTGAIWVAP
                     VQGAEHFDLVEHSARELDAMGFEMMALGSPVELMEAYEFAMLARMIISAKRIIPAKPI
                     HLFGAGHPLTIPLAVALGCDTFDSASYMLYAKDNRYMTPNGTVRLDELAYLSCQCPVC
                     SSYTVQELYKMEIDKRTIEIAKHNLYILKAEVEAVKQAIMDGRLWEYVMQKARAHPKL
                     MEAMQIFKDVEMLEDGTPLFKERAIFFYDAIDQHRPEAKRFRKTVARFQSSKKKLILC
                     PEAEIHPFYSTTSYKDIVKKFPDAQVCS"
     gene            complement(1265..1678)
                     /locus_tag="VE308_03390"
     CDS             complement(1265..1678)
                     /locus_tag="VE308_03390"
                     /inference="COORDINATES: protein motif:HMM:NF026244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsm family RNA-binding protein"
                     /protein_id="HZB00050.1"
                     /translation="MAAVVTRKFGEEALQFLGKKVSVETSDQKIYSGTLAGIDERLDV
                     VLDKVEGHGILKVIINGSFVKEVRLMEKPFDFKALAERLSRVFPGLVKIREDVGAIIV
                     MDKIKVTQAGIEEGSGLAADRVKAIYDEFTKETRK"
BASE COUNT          515 a          506 c          420 g          666 t
ORIGIN      
        1 tagctgcaga cctgcgcatc agggaatttc tttacaatat ctttgtaact tgtagtggag
       61 tagaatggat gaatttcggc ttcaggacaa aggattaatt tttttttcga cgattgaaat
      121 cttgcaaccg tcttcctgaa ccttttagcc tcgggcctat gttggtcaat tgcatcataa
      181 aaaaatatag ctctctcttt gaagagcggc gtgccgtcct ccagcatttc gacatccttg
      241 aagatctgca ttgcttccat taactttggg tgtgctcgcg ctttctgcat aacatattcc
      301 caaagcctgc cgtccattat tgcttgcttg acagcttcca cctccgcctt taggatatag
      361 aggttgtgtt ttgcaatttc aatggttcgc ttatctattt ccatcttgta caattcctgc
      421 actgtgtatg atgagcaaac tgggcattgg cacgataagt aagccaattc atcgagccta
      481 actgtcccat ttggggtcat gtacctattg tccttcgcat atagcatgta ggaagcagaa
      541 tcaaaagtat cgcaaccaag tgcaacagca agcgggatag tgagtgggtg accggcgcca
      601 aagaggtgaa tcggttttgc agggattatt ctttttgcag atattatcat gcgtgcaagc
      661 atagcaaact cgtatgcttc catcagttca acaggactgc caagcgccat catctcaaaa
      721 cccattgcgt caagctctct tgcagaatgt tcaacaagat caaagtgctc ggccccttgc
      781 acaggcgcaa cccatatcgc ccctgtgtta cctaatactc gcaggctctc tagcgcattt
      841 tttagcgtaa tcgcaacata ttcttttgcc tttttatgtt caaggccata gcctgtcggc
      901 ctatcaagag ggataggtat gtcgcttttg atgtcatttt caaactgggc catcattgca
      961 ggctcaactt cgacagagcc atactcaagc acctggtaac cacccgaatc agtcatgaca
     1021 gccccatcat atttaatgat gttatgtatt cctcttcttc gtgcctcgtc gccatagtga
     1081 cggagagtta tgtaagcatt tgtaatcaaa agatcaaagc ccatcttttt caaaaattgt
     1141 gtgctgaccg tctgtcttac tgggtggact accggcacaa aggctggcgt ctcaacaacg
     1201 ccgtgtagcg tctgaagcct tcctatgcga gctgctagat ctgagtgcca gacctcaaac
     1261 acaactactt ccttgtctcc ttcgtgaatt cgtcatagat agccttaacc ctatcagcgg
     1321 caagcccaga accttcctca atgccagctt gcgttacctt gatcttgtcc atgacgatta
     1381 ttgcaccaac atcttctcgt atcttgacaa gaccagggaa aactcttgag agtctctctg
     1441 caagcgcctt aaagtcaaat ggtttttcca tcaatcttac ttccttgaca aatgagccgt
     1501 tgataatcac tttcaggatt ccatgccctt ccactttatc gagcacaaca tcaagccttt
     1561 catctattcc agcaagcgtt ccactgtaaa ttttttgatc cgaagtttcc actgagacct
     1621 tttttccaag gaactggagt gcctcctctc caaatttgcg tgttacaact gcagccatgc
     1681 taatcggtat cgtcgagtat tatatatacg aattttcgta acatgccctg tctaaccgac
     1741 gcttctgtat tgtttttggg ggagagttct cattgctctg tgtgatattg atatatatgt
     1801 acgtcttgtt gtcgttgctc tgtcggatct ctgtatttat ttattatttt ttacattagc
     1861 tttttttctg actgcgacaa gaatatcttt gagccacttg ctttgcatta atgttcactg
     1921 tcgtaatagt tatacgaatt gctttgcttc gcttctatag agtgacaaac tgctggcgcc
     1981 agtcattccc gtgggcgtca tgtcagtcat acgttctatg ctgctgattg aaaactctac
     2041 tgcgggttaa gatgtaaaga ttagtatttg aaaaatctcc gcatgtcgcc cttttctgat
     2101 acaaagt
//
LOCUS       DASQTF010000143         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_227062,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000143 DASQTF010000000
VERSION     DASQTF010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227062"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..1370)
                     /locus_tag="VE308_03395"
     CDS             complement(315..1370)
                     /locus_tag="VE308_03395"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HZB00051.1"
                     /translation="MIILVRVFINGYGNIGRRLASALAVDKEIQLVGVAKYSIDDKVK
                     EALENRYNVYVPNESIGNFKEQGYNVTGSIKEALERCDLVVDAAKEGVGYDNKKKFYE
                     PMNKPAIFQGGEERNGERAVADMIHNSRVNYDKAAGKTYVIQGSCNVSGMGRVMQPLI
                     QKFGDRIRRFDIELVRRWADLEDTKAVKDSIEWDRNPHHQDDVRDFIPNANLYVDAFK
                     VPSRMMHLHHMFVRFNNGAVPTKDEILECFRDEFGVAIISSAKGTGDVRKKALELGFP
                     HGDTNMVHIHQDILRVQDDTIKIAYSDDQTGMVIPENHLLIQSMVFRRHREEAIKRAD
                     ILFQMSKKRAILEEEFK"
     gene            1572..2159
                     /locus_tag="VE308_03400"
     CDS             1572..2159
                     /locus_tag="VE308_03400"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZB00052.1"
                     /translation="MKILVIDNYDSFVYNLAQLMGELRTQPTVLRNDRITLEDVVKMD
                     PDAIVISPGPGHPADRKYFGVCTDVIRELGHRIPILGVCLGHQGIVHAFGGKVINAKK
                     IRHGKTSMVQYTADRLFEDVANPFKATRYHSLVADQNNLPSCLEVTARAIDDGEIMGI
                     RHRHYLMEGVQFHPESVLTGEGRRMLLNFISMVKK"
     gene            2156..>2377
                     /locus_tag="VE308_03405"
     CDS             2156..>2377
                     /locus_tag="VE308_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00053.1"
                     /translation="MTISTDLRPEIKKLVAKTDLSPAEITHALDAILAALVPETAVAA
                     FLVALAMKGETPDELRSILVSLRNHATSIT"
BASE COUNT          646 a          537 c          473 g          721 t
ORIGIN      
        1 aagtgtatat ttgtgctgtc aattaatgaa cctctcaaaa aagcgctgct actcatatca
       61 gagctcactt cctgacatct catacgtcct gcagtattaa gcaagcttgt ttctgtaaga
      121 gttactatag atatgatggc aaactttatc ctttagaata tacagcgtat atttttagat
      181 ctagggaaaa ccaagaaaac tagaatttga atgacaacta ggattctgaa gagcggtatt
      241 aagcacccat catcttatca tttctctttg atcgatgaaa aagggaaaaa taatttaaag
      301 cttggataaa aatattattt aaattcctct tctaatattg cgcgcttctt gctcatctga
      361 aacagaatgt cagctctctt aattgcctcc tctctatgtc gcctaaatac catcgattga
      421 attagcaaat ggttctccgg tatcaccatg ccggtctggt cgtccgagta agctatcttg
      481 attgtgtcat cttgcactct caagatgtct tggtgaatat gcaccatgtt ggtgtcacca
      541 tgaggaaatc ctagctcaag cgcctttttt ctgacatctc cggtgccctt ggcagagctg
      601 ataattgcga ctccaaactc atctcggaag cattctagta tctcatcttt ggtcggcacc
      661 gcaccgttgt taaaccgaac aaacatgtgg tgtagatgca tcatccttga gggtaccttg
      721 aaagcatcga catacaggtt agcgttagga ataaagtctc tgacatcgtc ttgatgatgg
      781 ggattcctat cccattcaat tgagtccttg acggccttag tgtcttcaag gtctgcccac
      841 cttctgacaa gttcgatatc aaagcgcctg atcctatcgc caaacttttg aatcagtggc
      901 tgcattaccc tgcccatccc ggacacatta cagctgcctt gtatgacata tgtcttgcct
      961 gcagccttgt cgtagttgac acgtgagtta tggatcatat ctgctactgc tcgttcgcca
     1021 ttcctttctt ctcctccctg aaatattgca ggcttgttca taggctcata gaatttcttc
     1081 ttattgtcgt accctactcc ttccttcgcc gcatctacca ctaaatcgca cctctctagt
     1141 gcctctttaa ttgagcctgt cacgttatag ccttgttctt tgaagttccc gattgattcg
     1201 ttcggcacgt acacattata acggttttcc agtgcctctt tcactttgtc gtctatgcta
     1261 tatttcgcaa ctccaactaa ctggatctct ttgtcgactg ctagcgcgct tgcaagacgg
     1321 cgccctatgt ttccataccc attaatgaag actctgacca aaataatcac tcgggattta
     1381 gtatgaaact aaaatagctc gcatctatta tatagattcc taaatccttt agcagatttc
     1441 ttgtcgatgc agtaaactta aatactgtaa aggtctccat aattgtggag accatgcaaa
     1501 gaggcggtcg attttggcac ctttaaagat ctcgttataa ctgattcttt agcttcaggt
     1561 aggtgttttg gatgaaaata ctcgttatcg acaattacga ctcttttgta tacaatcttg
     1621 ctcagctaat gggtgaacta agaactcagc ccacagtctt aagaaatgat aggataacac
     1681 tagaagatgt tgttaaaatg gatcctgatg caatagtgat atctcctggc ccgggtcatc
     1741 cggcagaccg taaatacttt ggtgtgtgta cagatgtaat cagggaactg ggacatagga
     1801 tcccgatatt aggagtctgc cttggacatc aaggcattgt ccatgctttt ggtggcaaag
     1861 tcataaatgc caagaaaatc aggcatggca aaacaagcat ggtacaatat acagccgaca
     1921 ggctctttga agatgttgca aatccattca aggctacccg ctatcattct cttgtagctg
     1981 atcagaataa cctcccatca tgccttgaag tgactgcaag agcaatagat gatggcgaga
     2041 taatgggcat cagacatagg cattacctta tggaaggcgt tcaatttcat cctgagtctg
     2101 tcctcacagg cgaagggcga aggatgctcc tgaactttat atcgatggtg aaaaaatgac
     2161 catatcgact gatttgcgac ctgaaataaa aaaactggtc gcaaagactg atctatcgcc
     2221 tgcagaaatt actcatgcgc ttgatgcaat ccttgctgca ctggttcctg aaacggctgt
     2281 tgcggctttt cttgtagctc ttgcaatgaa aggagagaca cctgatgagc ttagatcaat
     2341 ccttgtttcg ttgagaaacc atgcaactag tataact
//
LOCUS       DASQTF010000144         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_121213,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000144 DASQTF010000000
VERSION     DASQTF010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121213"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            673..1587
                     /locus_tag="VE308_03410"
     CDS             673..1587
                     /locus_tag="VE308_03410"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HZB00054.1"
                     /translation="MLSVLMPASKGKTLIEVTVVGPDRKGVVADITNFIFKRGGNIEK
                     INQNVLRGLFGMQFEASFAEEIGRKQLDLGLKQLAKKLSMEIKVHYQEPNRLQNVAIF
                     VSKEPQCLEGLLQAKKRGQIRANISVIIGSDDTLKSIADDFGIPFHPVVHRDQAEAEN
                     RILQLIEEYNIDLIVLARYMRILTPNFVWHYPNRIINIHPSLLPAFPGAYAYVQAYER
                     GARIIGCTAHFVTEDLDQGPIIHQESFKVSTSDTLDTIKKKGQELEAATLLEGVRLYL
                     ENKLEVYWGKVYVKEDKKIDQSQKTKGS"
     gene            1613..2332
                     /locus_tag="VE308_03415"
     CDS             1613..2332
                     /locus_tag="VE308_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZB00055.1"
                     /translation="MSAPVFCMTDYDFRITLKKIKRAIIPVGSLEQHGAHLPVSTDSL
                     IAEYIARLLAEKVGAFVLPVISYGVSFEHKPMFNVSLHNSTLSKVLCDACISLAENRL
                     REIIILNGHHGNTGALQYISQELQSKIPTHVNIHTLHYWQMMTPEFDHAGEVETSLVL
                     AIAPELVHMDRAMPNSKNLSKSRAAYSSLTNAPGSFPRITGNGVWGDPMKATASKGDK
                     LIKELLANLTKTIAELSGQSC"
     gene            2646..>3524
                     /gene="thsB"
                     /locus_tag="VE308_03420"
     CDS             2646..>3524
                     /gene="thsB"
                     /locus_tag="VE308_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HZB00056.1"
                     /translation="MSIQQQQPMPIVLLREGTSETKGNQAQRNNITAAKTIAEIVRTS
                     LGPRGMDKMLVNNLGDVTITNDGATILKEIDVQHPAAKIMVEISKATDNEVGDGTTST
                     VVLAGSLLSKAEELIAKGVHPTVIVDGYSMASEKALEILRNASTKVDPADRQILGKIA
                     RTSMASKLVSVNSEPLSKIVVDAVIAVAEKARDNNNLKVDVDSIKVEKKAGVSIEDTK
                     LIQGIVLDKEVVHGAMPKRIEDAKIALLNAALEIEKTEMSAEIRISDPQQMHMFLEEE
                     NRMLKSMVDKITASGAN"
BASE COUNT         1112 a          723 c          752 g          937 t
ORIGIN      
        1 caaaaaatta cgtagtgtaa atgaatgaag taaccactgc tttgtcttta aaaaagataa
       61 gatttaaagc tttttcattg tcacttaacg tagcttctac attctagcca acctgttctg
      121 cttagcgccg tttatagtct catcttttag cattttcagg gtgtgattct gtttatattt
      181 caggattaaa ctgttttcaa acagtacatc gccgatatta aaaataccta tgtgaaatac
      241 ttgagaatta tattagcaca ggtcttttgc gatcctcctc tcttaatgcg ccactaaagt
      301 attggttaat caatgcgtca gcctgtagtg cacagtttct tttctttatg gtcttgaaaa
      361 tgcatctggc aaaagtcatt gtcgcagtcg tagcaatgcc aaccggaaat ggtgtaacag
      421 actatacact tccctatggg tttgttatag tcgtagtttt ttctttgttt tgcaggagat
      481 ggtttttgtt gttttggctg tgccttcata tgccatatgc ctcagagggc tagagaaata
      541 ttacagaagc gcatcttcta cagtcctcaa aaattataac cgttgttaac gctgtaaccc
      601 aacggttttc aattaacaat cctattattt aagcattgac ggcgcatctg aacatttatt
      661 tatgtttcag aaatgctatc agttctcatg cctgcatcca agggcaagac tctcattgag
      721 gtaactgttg tggggccgga caggaaaggc gtcgtagccg atattaccaa ctttatcttc
      781 aaaagaggcg gtaacatcga aaagataaac caaaatgtgc tccgcggcct ctttggcatg
      841 cagtttgaag catcatttgc agaggagatc ggaaggaagc agcttgacct tggtctaaag
      901 cagcttgcca aaaagctgtc aatggaaata aaagttcatt accaagagcc taaccgactc
      961 caaaatgttg caatctttgt aagtaaagaa cctcaatgtc ttgaggggct actgcaagct
     1021 aagaagaggg gccaaataag agccaatatc tctgttatca ttggaagcga cgacacgctc
     1081 aaatcaattg cagatgattt tggaataccg tttcacccag tagttcatag agatcaggca
     1141 gaggcggaaa acagaatcct gcaactaatc gaggagtaca atattgatct gatagtcctc
     1201 gcaaggtata tgcgtatact gacgccgaat tttgtttggc attatccaaa caggataatc
     1261 aatatacatc cgtcacttct gcctgcattt ccaggggcat atgcatatgt tcaggcatat
     1321 gagcggggcg ccaggataat agggtgcact gcacactttg taactgaaga tcttgaccaa
     1381 ggtccaataa tccaccaaga atcgttcaag gtatcgacct ccgacacact tgacaccatc
     1441 aagaaaaagg gccaggaact tgaagcggcg acccttcttg aaggagtcag actctacctt
     1501 gaaaacaaac ttgaagtgta ctggggaaag gtctacgtca aagaagataa aaaaattgat
     1561 cagtcacaaa aaacaaaagg tagttaatgg gcacaagcct gcttattgca taatgtcagc
     1621 accggtattt tgcatgaccg actatgattt tagaataaca ctcaaaaaaa tcaagcgagc
     1681 tataatacct gtgggttccc tcgagcagca tggtgcgcat ttgccggtat cgactgattc
     1741 acttatcgct gaatacattg caagactctt agcagaaaag gttggcgcat ttgtcctgcc
     1801 agtgatttca tatggtgtat catttgagca taaaccaatg ttcaatgttt ctcttcacaa
     1861 ctcgactcta tctaaggtgt tatgcgacgc gtgtatttca cttgcagaaa accgtttaag
     1921 ggaaataatc atcctaaatg gccatcacgg taacacgggg gcacttcagt acatttcgca
     1981 ggagctgcag agcaagatac ctacgcatgt caatatccac accctacatt actggcagat
     2041 gatgacgccg gagtttgacc atgcaggcga agttgaaaca tctcttgtgt tggcaattgc
     2101 gcctgaactt gttcatatgg accgagccat gccaaattct aaaaatctat ccaagtcaag
     2161 agcagcttac agcagcctca ctaacgcccc agggtctttt cccaggatta ccggcaatgg
     2221 cgtttgggga gacccgatga aggccacagc ttcaaaagga gacaaactga taaaagaact
     2281 ccttgcaaac ctgaccaaaa caatagccga gctcagcgga caatcgtgtt aattattatt
     2341 gtacttgtta tatactttag aagaagataa taatatcatc ttttcaaaat actctcctat
     2401 ctactgtaca gggctattca attacctttg gagttgccaa agaagctgcc tagcatacat
     2461 tgattatagt aatcacagtt ttatcatata ttatcctatg actttcaact actagcttat
     2521 tcgatattaa attcatataa taggcatata gccattatct gtatctgatg aagaggctag
     2581 tagaagatct taaattacgc ctaaaagatc atctctgggt actaacctaa ggtgagatat
     2641 gatctatgtc aatacaacaa caacaaccta tgcctattgt ccttttaaga gagggcacca
     2701 gtgaaacaaa gggcaaccag gcacagagaa acaacatcac tgcagcaaag acaatagcag
     2761 aaattgtgcg cacaagtctt ggcccaagag gaatggacaa gatgcttgtt aacaatctcg
     2821 gagatgtaac aataacaaat gacggtgcta caattctcaa agagatagac gttcagcatc
     2881 cagcagccaa gataatggta gagataagca aggcaacaga caatgaggtg ggagatggta
     2941 caacctcaac agttgttctt gcggggtcgc tgttgtctaa ggctgaagaa ctgatagcaa
     3001 agggcgttca tcctactgtc atagtcgacg gttacagcat ggcatctgaa aaagctcttg
     3061 agatcctaag gaacgcatca acgaaggttg atcctgcaga cagacagata cttggcaaga
     3121 tagcgcgtac aagcatggct tccaaacttg tctccgtgaa ctcagagcct ctgtcgaaga
     3181 tagtggtgga tgcagtcatt gctgtagcag aaaaggctag ggataataat aacctcaaag
     3241 ttgacgttga tagcataaag gtcgaaaaga aggcaggagt atccatagaa gataccaagc
     3301 tgattcaagg tattgtgctt gacaaagaag ttgtacatgg ggccatgcca aagcgtattg
     3361 aggatgcaaa gatagcttta ctcaatgctg cattagaaat cgaaaagaca gaaatgagtg
     3421 cagagatccg catttctgac ccacagcaga tgcatatgtt cttggaagag gagaatcgca
     3481 tgctcaaatc tatggtagat aaaataaccg cttcaggcgc aaat
//
LOCUS       DASQTF010000145         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_229654,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000145 DASQTF010000000
VERSION     DASQTF010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229654"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VE308_03425"
     CDS             <1..282
                     /locus_tag="VE308_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00057.1"
                     /translation="EGNSNNTLYLMNITADYKIASANGTELQLIEDMPPVVNITSHRQ
                     NTEMFLTLTLTQDVQSLPTGSYVITYSVTDEVSGESFQLEREITVAERE"
     gene            complement(303..977)
                     /locus_tag="VE308_03430"
     CDS             complement(303..977)
                     /locus_tag="VE308_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00058.1"
                     /translation="MPKYNLCVIFYVPKASSVLVIAVAVATLIPASIGLSGLGPEIKI
                     GGRQTVGIYNNADSSIEDARGDVTPVYQQVGIEEIPEVRDYHDLLGASVQKRGEVFFF
                     TVALAGNPNDNDMYETQYRWHIITTSPITNREQQYIVRFQNSAFGSGNSTTMQGWYFS
                     VFDRNVGRFIVPPTSIPDMPEDRVEFPIEDFYIGNPSSFSYWVDVSVRVNATFGQPNY
                     IMDYAP"
     gene            1025..2716
                     /locus_tag="VE308_03435"
     CDS             1025..2716
                     /locus_tag="VE308_03435"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZB00059.1"
                     /translation="MPASEFSHFVEMCEKVRQTASKNAKVAAIAEYLTRPKDDDDSSS
                     SDTSLAAAALFMSGAIFPKGSGLTLNVGFNTIMRSLSEIASLEPEEIQKIYLKHGDMG
                     ALAEYVVSKRQQSPLVVQQLLLPDLYDQLRKIADAAGSGAAEAKRKILTGLLINSSPL
                     EAKYLIKIATGELRVGVVEGLVELAIAKAFGRDVHAIRQAMLLSGDIAQVALLAKKDA
                     LSTAIMKPLVPLSFMLADVMFSAEEIEQYYNKPLICEFKYDGIRVQLHKFGGKVKMFS
                     RNLEDVASSFPEIVSTVSLLPAGFILDGEVVAYRDGRPLHFQELQKRLRRKAVTVHLM
                     GEIPVVYVPYDIMYLDGQPLIDRPLTERKKLLSQIRFKEPVIDLGYRVVSTAQEIAYA
                     FKESRDAGHEGLVVKELESQYHPGKRGRHWTKLKYELDTVDAVIVIAEYGHGKRAGTL
                     SDYTFAVRDEHDGGSLKTIGKAYSGLTDAEIAKMTDKLRSIAVRDEGYRLVVRPEIVL
                     EVAFDSIQKSDRHNSGFALRFPRIKYIRTDKTVRDIDTLEKVQKIYERQSYLMSR"
     gene            3095..>3680
                     /locus_tag="VE308_03440"
     CDS             3095..>3680
                     /locus_tag="VE308_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00060.1"
                     /translation="MLKAIILTGAVGVALVVYFSLSLYVFPQSYQSIEAQRPQAIVTD
                     VSLSAPDIALGQALTISVTGANNGEDADMQIVSVGFPNLTTTDNIKVLKHNFAQTPIF
                     IAPGELLSSEYSDIDSSVLAQYTSIEAFSRPWEAGKTYSTDIEVIPKTEGRFVVFIKS
                     VALPHTWEQAHYPQDGTLDQQKEFVEIYAVQVTKP"
BASE COUNT         1041 a          798 c          882 g          959 t
ORIGIN      
        1 gaaggcaata gtaataatac actttacctg atgaacataa ctgctgatta taaaatagca
       61 agtgcaaatg gcaccgagct tcagcttata gaggatatgc cgccggtggt aaacattact
      121 tcgcacagac aaaacaccga gatgttctta acgctgaccc ttacgcagga tgttcagtcg
      181 cttccaacag gcagctatgt tataacatat tctgtgactg atgaggtgtc aggtgagagc
      241 ttccagctag aaagagagat cacagtagcc gagagagagt gatatcgtcg agccttacat
      301 gatcacggcg catagtccat tatgtagttt ggctgaccga aggtagcatt gacccttact
      361 gacacatcga cccagtagga aaatgacgag gggttgccga tataaaagtc ctctatagga
      421 aactcgactc tgtcctctgg catgtcgggg atactcgtag gtgggacaat aaaccttccg
      481 acattcctgt caaatactga gaaataccac ccttgcatag tagtagagtt gccactgccg
      541 aaagcagaat tttgaaatcg aactatatat tgttgctctc tatttgttat cggactggtg
      601 gtgataatat gccaccgata ctgtgtttca tacatgtcgt tgtcatttgg attgcctgca
      661 agcgctactg tgaaaaagaa tacttctcct cgcttttgca cgctggctcc caagaggtcg
      721 tggtagtctc ttacctcggg gatctcttct atcccaacct gctggtatac tggcgtcaca
      781 tcacctcgcg catcttctat actgctgtca gcattgttat agattccgac agtttgcctc
      841 ccgcctatct ttatctccgg ccccaagcca gacagcccaa ttgaagctgg gataagcgtt
      901 gcgactgcaa ctgcaataac cagaacagac gatgccttgg gcacataaaa aatcacacag
      961 aggttgtatt tcggcatttt gccaagagat ttaagtacag ttgaagactg gatatacgca
     1021 gaatatgcct gccagcgaat tttcgcactt tgtcgaaatg tgtgagaaag ttcgtcagac
     1081 tgcaagcaaa aatgccaagg tggctgccat agcagaatac ttgaccaggc cgaaagacga
     1141 cgacgacagc agcagcagcg acacctcgct tgcagcagct gcactcttta tgtctggtgc
     1201 catctttcct aaaggctctg gtcttacact caatgtaggc tttaatacga taatgcggtc
     1261 gctttcagaa atcgcaagtc tggagccaga agagatacaa aagatctact taaagcacgg
     1321 agatatgggc gctcttgctg aatatgtagt ttccaagagg cagcaaagcc cacttgtggt
     1381 tcagcaactg ttgctgccag atctatacga ccagctaaga aaaatagctg atgcagctgg
     1441 ctctggggca gcagaggcaa aaaggaagat cctcactgga cttctcatca actcctctcc
     1501 gcttgaggca aaatatctga tcaagatagc aaccggcgag ctgcgcgtcg gtgtggtaga
     1561 gggcttggtc gagcttgcta ttgccaaggc ttttgggcgg gacgtgcatg caattcggca
     1621 ggctatgcta ctgtccggcg atattgcaca agtagcactc cttgcaaaaa aggatgcttt
     1681 atcaactgcg ataatgaagc cacttgtgcc tctcagcttc atgctcgcag atgtgatgtt
     1741 tagtgcagaa gagatcgagc agtattataa caaaccgctg atttgtgagt tcaagtatga
     1801 cggcataaga gttcaacttc ataaattcgg cggaaaggtc aaaatgttct caaggaatct
     1861 agaagatgtt gccagctctt ttcccgagat tgtatctact gtatctttgc tgccagcagg
     1921 atttattctg gatggcgaag tggttgccta cagagatggc aggccacttc atttccaaga
     1981 gttgcaaaag aggctaagga gaaaagcagt tacagttcat cttatgggcg agatcccagt
     2041 agtgtacgtc ccctatgata tcatgtacct tgacgggcag ccgctcattg acaggccgct
     2101 tacagaacgc aagaaattgc tctcgcagat tagattcaaa gagcccgtaa tcgaccttgg
     2161 ttacagggtt gtgtcgacag ctcaggagat agcctatgca tttaaagaaa gcagagatgc
     2221 ggggcatgaa ggattggtgg taaaagagct tgaatcccag taccatcctg gcaagcgggg
     2281 aagacattgg accaaactaa agtatgagct tgacactgtc gatgcagtaa ttgtcattgc
     2341 agagtatggt catggtaagc gtgctgggac gctttctgat tatactttcg cagtccgaga
     2401 tgagcatgac ggtggttcgc taaagacaat aggcaaagcg tactctggcc tgaccgatgc
     2461 agagatcgcc aagatgactg acaaactacg gtcaatagca gttagagatg agggctatag
     2521 actagtagtc aggcccgaga ttgtccttga ggttgcattt gatagtatac agaaaagtga
     2581 taggcataac agtgggtttg cgcttcgctt cccgaggata aagtatatcc ggactgacaa
     2641 gactgtacgt gatattgata ccctggaaaa ggtgcaaaag atctatgagc gtcagtcgta
     2701 tctaatgagc cgctagcctg ctatatgtgt cgctatcttt gttaggtagt tttggagcgc
     2761 gcatttgcgg catgcatatt aacttacacg aatctacttt gtacacaaaa aagagagaga
     2821 aaacacaaca tgtataaaag attcaagcac aaattcctac gaagtatagc aagggagacc
     2881 gcgatcttat tctgaactgt actacctaca cacagtcatg tagcatctag aggtagtagt
     2941 agcatctgca taatttagga ccaatttctg cgtgctcaga tgcacctatg attttttgta
     3001 tgtgtatgtg tgtatgtatg tatggctatg tctatgtatg atttgcatag tgctaagaaa
     3061 taaaagatca atagtgtaat ttacattcac tgcattgtta aaggcgatca ttcttactgg
     3121 cgcagttgga gttgcactgg tggtatattt ctcactctcg ctttatgttt tccctcagtc
     3181 ataccaatcg atagaggctc aaagacctca agcgattgta acagatgtga gtctatctgc
     3241 acctgacatt gctctaggtc aggcgctcac tatctctgtt actggggcaa ataatggcga
     3301 ggacgccgac atgcagattg tatctgtagg atttccaaac cttacaacca ctgacaacat
     3361 caaggtacta aaacacaact ttgctcagac gccgatattt atagcgcccg gcgagctgtt
     3421 gagctctgaa tattccgata tagactcttc tgtattggcg caatatactt caattgaagc
     3481 tttcagcaga ccgtgggagg caggcaagac ctacagcaca gatatagagg tcattccaaa
     3541 gactgaggga aggtttgtgg tttttatcaa atctgtcgca ctgccccata cttgggaaca
     3601 agcgcattac ccacaggatg gcaccctcga tcaacagaaa gagtttgttg aaatatatgc
     3661 agtacaagtg acaaagcctt
//
LOCUS       DASQTF010000146         3744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1236531,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000146 DASQTF010000000
VERSION     DASQTF010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236531"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VE308_03445"
     CDS             <1..300
                     /locus_tag="VE308_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZB00061.1"
                     /translation="EKHFFEHYHRVMVVITAGFIFATAVYSFKKFKPARKTAIIAAIV
                     VSVQIIFGMLVVTTRLEPFLVASHLSTGILLFAMTLMTFLASIKLKYNGGRWRII"
     gene            complement(718..900)
                     /locus_tag="VE308_03450"
     CDS             complement(718..900)
                     /locus_tag="VE308_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00062.1"
                     /translation="MASKNQDEGKVLLTLSNIKYLVEGLEALLTTDLDGDKRAKVIQL
                     RNDLLSHINSIFNDYS"
     gene            1022..1954
                     /locus_tag="VE308_03455"
     CDS             1022..1954
                     /locus_tag="VE308_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZB00063.1"
                     /translation="MAVSQQQTIINLYNSGISPEIIAFQLDISPEDVDRVLQSVNKTD
                     RPAKQAPEAGHLASFYLDAVVDLDKAIKMAQQRVWKALKVESKFDISTEETHEILAKY
                     SKSKVMLVILHIDLVGSTRLSSDLPTDRLAAIIQAFTQEMSITISAYGGYVLKYVGDA
                     IMAFFLATNDKYLPSINAVNCAHSMIKIIREGINPILNQYDYAEMNVRVGIDLGENVV
                     VQYGWDTFNIDGKKFKRPHHDILGHTINIATKMTSLGKPDQIVIGQMVYDVLDDRQKS
                     TFHRLQVSADVWGYMSDYSSGIYPLYGTSGAYMH"
     gene            2040..2516
                     /locus_tag="VE308_03460"
     CDS             2040..2516
                     /locus_tag="VE308_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HZB00064.1"
                     /translation="MIGGEEYIKVARALLNTKDVTDEEIASATGLKINIVRKVLYDMF
                     GKALITGIRVKDEKKGWFVYRWRAKQDQVDNFIDNQKKKILDRLHNRLEYEESTEFYH
                     CGNRDCPRVKFDVAVELFFKCPNCKGSLNMIDNTKIKEALHHKIDQVTTDINSNKK"
     gene            complement(2697..3389)
                     /locus_tag="VE308_03465"
     CDS             complement(2697..3389)
                     /locus_tag="VE308_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZB00065.1"
                     /translation="MLPGSYDKMHWLNYKVIGCTRCPRLSQYIRQIGQEKVKRFAREE
                     YWARPLPSWGDPNARLLIVGLAPAAHGGNRTGRMFTGDGSGDWLARAMYESGFANMPT
                     SRSKDDGLVLKDAYITAAIRCAPPDNKPLPTELVNCSQYLVTELQILYNIKVIIMLGK
                     IGFDAYCRAAGLRRLKFSHGASYDINSKVLLASYHPSRQNTNTGKLTWQMWLDIFKKA
                     RSILGSYGQRTT"
     gene            3452..>3744
                     /locus_tag="VE308_03470"
     CDS             3452..>3744
                     /locus_tag="VE308_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZB00066.1"
                     /translation="MSTNTSTSDDLTWIAGSVVLSDNPPQQLKYCRKKFGIKQADLAR
                     HMSLTPSVLSDYEKGRRPSPGVKFIKRYLLALYELAKSNGKYTEQVAPTPVASV"
BASE COUNT         1079 a          881 c          782 g         1002 t
ORIGIN      
        1 gagaagcatt tctttgagca ttatcataga gtgatggtgg tgataacagc tggttttatc
       61 tttgctaccg cagtatactc attcaaaaaa ttcaagccgg cgcgaaaaac tgcaatcatt
      121 gcggcaatag tcgtatcagt gcagataata tttggcatgc tggtggtcac tacccgcctt
      181 gagccttttc tcgtcgcaag tcatctgtca actggtatcc tgctttttgc catgacactc
      241 atgaccttcc tagcctccat aaaactcaag tataatggtg gccgttggcg tataatctaa
      301 ggcgtcaata tacattcatc gtataacgat agatcatctg ttttaaaaaa ggccataaaa
      361 aagagaggtg agttacatta agcattcttt ttatagatca aaagctaaaa acttgcttca
      421 tatctttgac ggcctatgtc agggcatgag ctttttcaaa aactctgcac taggtcttct
      481 aaagcacttg aatttgcgac tcagaagaat atgagtagtc ataactacta cttttgcgat
      541 gcgcagtata atatagctca attgtaccga gaaacttgat cctattgcgg ccaagtgcga
      601 tttcatattc tctgcattat caagtcccct agtattcacg ggatcattcg ggatgtgtga
      661 cctgataaaa aagagccatt gtaaacaaac aagccctcag caaggtccaa gcagtaatca
      721 cgaatagtcg ttaaatatgc tatttatatg tgacaagagg tcgttgcgca gctgaatcac
      781 cttagcacgc ttgtcgccat ccagatcagt cgtcagtaaa gcctcaagcc cttctaccaa
      841 atacttgatg tttgacagcg tcagcaggac ttttccttcg tcttgattct tgcttgccat
      901 tcctccccct ctatattact agcggcggtt attattatta ttattattat tctcggcgga
      961 agctttggtg caaatacaat aatagtatat atctaatact catgaccaaa gcttatcgta
     1021 tatggctgtg agccaacagc agactataat taacttgtac aattccggca tatcaccaga
     1081 gataattgca ttccagctcg acatcagccc agaagatgtc gacagggtac ttcaaagcgt
     1141 aaataagacg gataggcccg ccaagcaggc acctgaagct gggcacttgg catcttttta
     1201 cctcgatgcc gtagtcgacc ttgacaaggc gatcaagatg gcccagcaga gagtatggaa
     1261 ggcacttaag gtggaatcca agttcgacat ctctactgaa gaaactcatg aaattcttgc
     1321 caagtattcc aagtcaaagg ttatgctcgt gatcctccac atagaccttg taggctccac
     1381 tagactgtca tctgatctgc cgactgatag gctcgccgcc ataatccaag cgttcacaca
     1441 agaaatgtcg atcacaatat cagcgtatgg cggctatgtg ttgaaatatg taggagatgc
     1501 catcatggca tttttccttg caaccaatga caagtatcta ccatccatca atgcagtcaa
     1561 ctgcgcccac tcgatgatca agataatccg ggaaggcata aacccgatct tgaatcagta
     1621 cgactatgct gaaatgaacg tccgagttgg catagaccta ggtgaaaatg tcgtggtaca
     1681 atatggatgg gatactttca acatagacgg caaaaagttc aagagacccc accatgacat
     1741 cttggggcac accatcaata tagcaacaaa aatgacctcg cttggtaagc cggaccagat
     1801 agtaataggt cagatggtct atgatgtcct agacgatcga caaaaatcaa catttcaccg
     1861 gcttcaggta agcgctgatg tatggggcta tatgagcgac tactccagcg gcatttatcc
     1921 cctttacggt acatctgggg cttatatgca ttaagtatat atcagaaaaa cacgcatact
     1981 attaccatca ttagatgcac tccatagaat atgaggactc ttttgtcaaa attgctggct
     2041 tgatcggagg cgaggagtac atcaaagttg cccgcgccct cttgaacacc aaggatgtga
     2101 cagacgagga gattgcaagc gcaactggac taaagataaa catcgtacgc aaggttctct
     2161 acgacatgtt tggcaaagca ctgataactg gcatccgcgt taaggacgaa aagaaaggct
     2221 ggttcgtcta ccgctggcgc gccaagcagg atcaggtcga caatttcatt gacaatcaga
     2281 agaaaaagat ccttgaccgg ctccacaatc gccttgaata tgaagaatca acagagtttt
     2341 accactgcgg caaccgtgac tgtccacggg tgaaatttga cgtagcagtt gaattatttt
     2401 ttaagtgccc caactgtaaa ggctcactca acatgataga caataccaag attaaggaag
     2461 cgctgcacca caagatagac caagtaacta ctgacatcaa ttcaaacaaa aagtagatcc
     2521 tgtgctttgt gccatgactg catctctata caagtctcca atgacgcaat aatccgttct
     2581 gtgtatcctg tttttctaaa ttttttctag aagcaatggc ctcttttgac atgccgaccc
     2641 caacagcgga tggcaatcta tacttattag ttattgacct caagacaagt gaatggttat
     2701 gttgtcctct gtccataaga tcctaaaata gatcttgcct ttttgaaaat atcgagccac
     2761 atctgccatg tcagcttgcc agtgttagta ttctgcctgc ttggatgata tgaggcaagc
     2821 aataccttac tgtttatatc atagcttgcc ccgtggctaa acttgagcct tctcaatcct
     2881 gctgctcgac aataagcatc aaatccaatc ttgcctagca tgataatgac tttaatatta
     2941 tacaaaatct gcagctctgt aacaagatat tgagagcagt tcaccagctc ggttggcagt
     3001 ggcttgttat ctggtggtgc acatctaatc gccgcagtaa tgtaggcatc cttgagcaca
     3061 agcccgtcat cctttgacct gctggtcggc atgtttgcaa accctgattc atacatagcc
     3121 cttgcaagcc agtcgcccga gccatcacct gtaaacatcc ttcctgtcct gttgccgcca
     3181 tgtgctgcag gtgcaagccc aactatcagt agccgcgcat ttggatcgcc ccagcttggc
     3241 agaggccgcg cccagtactc ttcgcgcgca aacctcttga ctttttcttg gccaatctgc
     3301 ctgatgtatt gtgataatct tggacagcga gtgcacccaa tcaccttgta gtttagccaa
     3361 tgcattttgt catagctccc gggcaacact agcaagtatg cacacgttat tagaaaatct
     3421 ttttatcacc cgtcaaatgt ttgtttaaca tttgtctaca aatactagta ctagcgatga
     3481 cttgacatgg atagccggat ctgtagtatt gagcgacaac cctcctcagc agctaaaata
     3541 ttgtaggaaa aagttcggga tcaagcaggc agaccttgcg aggcatatga gcctcacacc
     3601 atcagtccta agtgattatg agaaaggccg caggccgtcg ccaggagtca agttcatcaa
     3661 gcgctacctg cttgcgctct acgagttagc caagtcaaat ggaaagtaca ctgaacaagt
     3721 ggcgccaacg ccagttgcca gtgt
//
LOCUS       DASQTF010000147         6622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_957307,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000147 DASQTF010000000
VERSION     DASQTF010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957307"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..346
                     /locus_tag="VE308_03475"
     CDS             83..346
                     /locus_tag="VE308_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZB00067.1"
                     /translation="MKYRSRTDITAQILEAANGGVTKTKIMYKAFLSYAQLKEYLTVL
                     IENGLLEYIEGEQIYRTTEKGNRFLKIYNQIGEYVAADMSEKK"
     gene            391..1134
                     /locus_tag="VE308_03480"
     CDS             391..1134
                     /locus_tag="VE308_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00068.1"
                     /translation="MSRKRRTAIRRVIIVTAIFATLGIGVSVALIAAGGGFETSSPNI
                     FDPPTEADIWKVGDHIQDGMTLEYALTSRGEVNSLESAQVSMNFDQAGENWNVRFTIA
                     NGTDQPPVDHTLTISKELTREGQLVESFRPYLEHIQSSILAVRDMEYGESPKYLVVGA
                     PWNTIFVGSSSVTARVTNEERVSTPAGVFDSFVLSYKLGEETSKIWVVRDMPLPVKAE
                     VYDAEDKLLYRYELTNASGVTSAAIGNSL"
     gene            complement(1140..1604)
                     /locus_tag="VE308_03485"
     CDS             complement(1140..1604)
                     /locus_tag="VE308_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00069.1"
                     /translation="MDDLHLNIKRNRDTLALALKRSPNTAYQKVNELAQFVGSHYSVD
                     LQLHFPIPGKISDLDSYGTENISLVVDKFRRVFPVPKETVKQKAVELIGSDAKPKDAY
                     TYEGKEGAKVIIIPKGSIEVLPGSMHFWCEIDRRIRSYGDWMIENVYFPKNP"
     gene            1697..2965
                     /locus_tag="VE308_03490"
     CDS             1697..2965
                     /locus_tag="VE308_03490"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZB00070.1"
                     /translation="MKSDQLFAKAKKFLPGGVNSPVRFYEPYPFFATSALGSKLTTAD
                     HQIYLDYCMGYGAIVLGHGYSSVIEAVRSQLDNGTLFCVPTECEIKLAEMICQIVPCG
                     EMARLVNTGSEATMHAIRLARAFTKRKGVIKFEGGYHGAHDYVLVKAGSGMASVPASE
                     GILEEASAHTKVVPYNNPAQLERAIEKNRNTACVIMEPVLANIGLVLPEKGYLNEVRK
                     ITEQNDIILIFDEVVTGFRLALGGASEFFGIMPDLVTFAKALGNGLPIAAIAGKKEIM
                     DKLAPAGSVYQASTFAGNPMSVAAGIATLETLTEAKNTIYPNMAKTCDSIVGGIKDML
                     ARAQLDCTVNSIGSMFQLFFTSERVKDEAGAKKANARMFRKLFDELLKQDIFIPPSQF
                     ETCFVSYAHTEDDADKTIEAYGKAFSEITK"
     gene            2962..3915
                     /gene="hemC"
                     /locus_tag="VE308_03495"
     CDS             2962..3915
                     /gene="hemC"
                     /locus_tag="VE308_03495"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZB00071.1"
                     /translation="MMMKKTIRVGTRGSRLAIAQTEIALAALRKAHPQIRFEVITIST
                     KGDVDKRPLFTLDEKGIFEKEVNEAVKKGEVDFAVHSLKDVPSDLSDDLSVACILRRA
                     SPNDVLVTDNGQKLKDLVPGSVVGTSSLRRAVQLTKTRPDLNVRPIRGNVETRVKKVI
                     SGEYDAAVLAEAGLARLGMKDVIVQRFSIRDFVPAPGQGAIAIVCRRDHAALAGMLQH
                     IEDRHSRIAVLAERALIRKIEGGCRFPLGAVAITNDDKITLYASVFSADGKQSIHIKK
                     VGRTSKPEELGSSVANMLIKQGAMNLAEGWRRAVDEWNKKL"
     gene            3897..4664
                     /gene="cobA"
                     /locus_tag="VE308_03500"
     CDS             3897..4664
                     /gene="cobA"
                     /locus_tag="VE308_03500"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZB00072.1"
                     /translation="MEQETMKKGKVFICGAGPGDPKLMTLRAMELLKTCDIVLYDRLV
                     SREIIDQIPAESEKAYVGRMVGDPATHQDMTNELMVKYTKKGKAVLRLKGGDPFIFGR
                     GAEEAEYLIKRGIEFEIVPGITSSIASPAYAGIPLTHRRYSSSVAIVTGHEDAKKDKL
                     VVRWDKLAGAVDTIVVLMGIAELDQISRNLIKAGMKKSIKVAIVASGTTDKQRIVKGT
                     LGTIAKLTKKADVKPPAVIVIGRVAGLSDKLDWFKRK"
     gene            4674..5450
                     /locus_tag="VE308_03505"
     CDS             4674..5450
                     /locus_tag="VE308_03505"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZB00073.1"
                     /translation="MPGLKNKILAITRSEHDAKEFLQLVREQGGRAIALPAIEIVPQG
                     PEVAEEFLDRLRKKKHYYCAFMSQQAVNVLFDLARDKIAPVLKSTTVIAVGPKTKQSL
                     EEHGIDVRLVLEKFSSFGLVDLLSGIEPAGKKIIIPRSRAANDFASEALTRLGMEVDE
                     IFLYTVRTRAIEPIWKEFYDLLLQKRVDAIIFTSASNVNSFFEIMDKVSKDELQLDSI
                     SKVISIGPFTSKALKDRGIECFEAEEHTVRGALQIAKQIL"
     gene            5484..5732
                     /locus_tag="VE308_03510"
     CDS             5484..5732
                     /locus_tag="VE308_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00074.1"
                     /translation="MTDGTTTKEAVLLEDSTFNVITQIEKKADFLYSSVEKYIRDAEK
                     DKKPELVKLWSTMKEDEQKHLKMLREQLVKEVKENKLQ"
     gene            complement(5982..6305)
                     /locus_tag="VE308_03515"
     CDS             complement(5982..6305)
                     /locus_tag="VE308_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HZB00075.1"
                     /translation="MSKLPTVNAEISIAPIGTSDTSMGKEIAAAYDAIRSIKNLKKVT
                     LTPMSTQVESDNLDDIFQAIHAAHNSAKSVGAKRVISTIRIDERLDKPNTLDEKVQSV
                     MTKAS"
BASE COUNT         1927 a         1395 c         1617 g         1683 t
ORIGIN      
        1 agttactcta tctatcatat agttaaaact tcgataataa aacttcgaga tgcttttata
       61 gatgttaata tctaggatag gcatgaaata tagaagcaga actgatataa ctgctcaaat
      121 tctagaagct gcaaatggcg gtgtcactaa aactaagatt atgtataaag ccttcctgtc
      181 atacgcacag ctgaaggaat acctgacagt gctgatagag aacggactgc tagagtatat
      241 cgaaggagag cagatctaca gaacaactga gaaaggtaat cgcttcctaa agatatacaa
      301 ccagattggc gaatatgtgg cagcagacat gtctgaaaag aagtaggtaa ataatctatt
      361 ttaaataacc aagatcactg aatcagaatc gtgtctcgaa agcggcgaac cgccatccgg
      421 cgggtgataa ttgtgaccgc catattcgca acattgggta ttggggtgag cgttgccctt
      481 attgctgcag gtggtggctt tgagacttcc agcccaaaca tcttcgaccc gcctacagag
      541 gcagacatat ggaaggttgg cgatcacata caggatggga tgaccttgga atatgcctta
      601 acttcaaggg gcgaggtaaa ttcacttgaa tctgcgcagg tatcgatgaa ttttgatcag
      661 gctggtgaaa attggaacgt aaggttcacg atcgcaaatg gcactgacca gccgcccgta
      721 gatcatacat taacaatctc aaaagagctg acacgagagg gtcagcttgt ggagtcattc
      781 cggccttacc ttgagcatat acaatcatca attcttgcag tccgcgacat ggagtatggc
      841 gaaagcccca agtatctcgt agtgggcgct ccgtggaaca caatatttgt tggttcgtct
      901 tcagtaacag ctagggtgac caacgaagag cgtgtgagca ctccggcagg ggtctttgat
      961 tcattcgtgc tcagttacaa gctcggagaa gagacgagca aaatctgggt cgttcgtgac
     1021 atgccgctac cagtcaaggc agaggtatat gacgcagagg acaagctgct ataccggtac
     1081 gagctcacaa atgcgtctgg cgtgacgtca gcagctatag gcaattcgct gtaaacaact
     1141 taaggattct ttggaaaata gacattttcg atcatccagt caccgtacga tctgatcctc
     1201 ctatcaatct cgcaccaaaa atgcatcgag ccgggcagga cttcgatact acccttgggt
     1261 atgattatta ccttggcgcc ctctttacct tcatatgtat aggcatcctt cggcttagca
     1321 tcactgccta tgagttcgac tgccttttgt ttcacagtct cttttggcac agggaaaacc
     1381 ctgcgaaatt tgtcaactac gaggctaata ttttcagtgc catatgagtc aagatcagag
     1441 atcttacctg gtattggaaa gtgcagttgt agatctacac tatagtgcga tccaacaaat
     1501 tgtgcaagct catttacctt ctgatacgcc gtgttgggac ttctctttaa tgctagagca
     1561 agagtgtcac ggttgcgttt gatattgaga tgcaagtcgt ccataaggct ggaaaatgtc
     1621 cccaatgcag tttcttacaa tctgtggtta attagcatta caattattat agctcttggt
     1681 agagatctat acatgattga aatctgatca gctctttgcc aaggcaaaaa aattcttgcc
     1741 tggcggggtt aacagcccgg tgcgctttta cgagccttat cctttctttg cgacctctgc
     1801 actaggaagc aagctcacta ctgccgatca tcagatctac cttgactact gcatgggcta
     1861 tggagctatt gttcttggtc atggctattc ttcggtaatt gaagctgtca ggtcccaact
     1921 tgataacggg accctctttt gcgtgcctac agaatgcgag atcaagcttg cagaaatgat
     1981 atgccagatt gtgccgtgcg gagaaatggc ccgtcttgta aatactggct ctgaagccac
     2041 aatgcatgcc atcaggcttg cccgcgcttt tacaaagaga aaaggggtga ttaaatttga
     2101 aggtggatac catggtgcgc atgattatgt tcttgtcaag gcaggatcag gcatggcgag
     2161 cgtacctgca tcagagggga tccttgaaga ggcgtcagct cacacgaaag ttgtgccgta
     2221 caacaaccca gctcagcttg agcgcgcaat tgaaaagaac cgtaacactg catgtgtcat
     2281 aatggaacct gtgcttgcaa acattgggct tgtgctacca gaaaagggat accttaatga
     2341 agtgaggaaa ataaccgagc agaacgatat tatcctgata tttgatgaag ttgtcactgg
     2401 ttttcgtctt gcgcttggcg gcgcgagtga gttctttgga atcatgccag atctggtcac
     2461 gtttgcaaag gcgcttggca acggtctgcc aatagctgca atcgcaggta agaaagagat
     2521 aatggacaag ctcgcacctg caggaagtgt ctatcaggca agtacatttg ctggcaatcc
     2581 tatgtctgtg gcggcaggca ttgctacatt ggaaacattg actgaagcca aaaatacgat
     2641 atatcccaat atggccaaaa catgcgatag cattgttgga ggcatcaaag atatgctggc
     2701 tagagcgcag cttgactgca ctgtcaattc cataggctcg atgtttcagc tattctttac
     2761 tagcgaaaga gtaaaggatg aagctggtgc caagaaggca aacgcaagaa tgtttcgcaa
     2821 attgtttgat gagctactaa agcaggatat cttcatccca ccttcacagt ttgaaacttg
     2881 ctttgtatcg tatgcacaca ctgaagacga cgcggacaag acaatagaag catacggcaa
     2941 ggcatttagt gagataacga aatgatgatg aagaagacaa tacgagtcgg aaccagaggt
     3001 agtagacttg caatagcgca gactgagata gcacttgccg ccttacgtaa ggctcacccc
     3061 caaatacgat ttgaagtgat cacgataagc accaagggtg atgttgataa gagaccgctc
     3121 tttaccctag acgaaaaggg aatcttcgag aaagaagtga acgaagcagt caagaaaggc
     3181 gaggttgact ttgcagtcca tagcctcaag gatgtcccat cagacctgtc agatgacctt
     3241 tcggtggcat gcattctcag gagggcgagt ccaaacgatg tgcttgtcac tgacaatgga
     3301 caaaaactga aagatctagt acctggctca gtcgttggca caagcagcct ccggcgggct
     3361 gtgcagctga caaaaactag gccagatctc aatgtcagac cgataagggg taacgtcgaa
     3421 acaagagtga aaaaggtaat aagcggcgag tatgatgcag ctgttctagc agaggctggc
     3481 cttgcgagat taggcatgaa ggatgttatt gtccagcgtt ttagcatcag agattttgta
     3541 ccagcgccag gtcagggggc tatagccatc gtgtgcaggc gtgatcacgc tgcacttgct
     3601 ggcatgctcc agcatataga ggatcgccat tcgcgcatag cagtgcttgc agaaagagca
     3661 ctgataagaa agatagaagg gggctgcagg ttcccccttg gagccgtggc aataaccaat
     3721 gatgacaaga taacgcttta tgcaagtgtg ttttctgcag atggcaagca gagtattcac
     3781 atcaaaaagg taggcagaac aagcaagcca gaagagctgg gctcctcggt agcaaacatg
     3841 ttgataaagc agggagcaat gaatctggca gaaggttggc gcagagccgt agacgagtgg
     3901 aacaagaaac tatgaaaaag ggtaaggtgt ttatctgtgg ggccggtccc ggcgatccca
     3961 aactgatgac tctgcgggca atggaacttt tgaagacctg cgatattgta ttgtatgacc
     4021 ggctggtgag cagggagata atcgaccaga tacctgcaga atccgaaaaa gcctatgttg
     4081 gcagaatggt tggcgaccca gccacacatc aagacatgac aaacgagctt atggtgaaat
     4141 acacgaaaaa aggtaaggcc gtactgcggc tcaagggcgg agacccattt atctttgggc
     4201 ggggggcaga ggaagctgaa tatcttatca agcgcgggat cgagtttgaa atagtacccg
     4261 gcattacttc gtctattgcc tcgccagcat acgccggcat tccgcttacc caccgtcgat
     4321 actcctcttc agttgccata gtcactgggc acgaagatgc aaaaaaagac aagctggtgg
     4381 ttagatggga caaacttgct ggtgcggtcg acactatagt cgtattgatg ggtattgccg
     4441 agctggacca aatttcacgc aatttgataa aggctgggat gaaaaagagc atcaaggttg
     4501 caattgttgc aagtggtaca actgataaac agaggattgt caagggtacg cttggcacca
     4561 ttgcaaaact tacaaaaaag gcggatgtca agcctcctgc agtaatcgtg atagggaggg
     4621 tcgctggact gagcgataag cttgactggt tcaagagaaa gtgacagata gaaatgcctg
     4681 gattaaagaa taaaatcctt gcaataactc gaagcgaaca cgacgcaaaa gaatttttgc
     4741 agctggtaag ggagcaaggc ggcagggcga tagcgcttcc cgcgatagag atagtaccac
     4801 agggcccgga agtggcagag gagttccttg acagactgcg caagaaaaaa cactattatt
     4861 gtgcgttcat gagccagcag gcagtgaacg tcctctttga ccttgcccgc gataagatag
     4921 caccagttct gaaatctact acagtaattg cagtgggccc aaagactaag cagagccttg
     4981 aagaacacgg catagatgtc agactagtac tagaaaaatt ctcttcattt ggactagtcg
     5041 acctcttgtc ggggatagag ccggccggaa aaaagataat catcccccga agccgtgctg
     5101 caaatgattt tgcctccgag gccctaactc gccttgggat ggaagttgac gaaattttcc
     5161 tctacaccgt gcgcacaagg gccatcgagc ccatatggaa ggaattttat gatctcttgt
     5221 tacaaaaaag ggtggatgcg attattttta ctagcgcctc aaatgttaac tcattctttg
     5281 aaataatgga caaggtgtcc aaagatgagt tacagctgga tagcattagc aaggtaattt
     5341 cgattggccc atttactagt aaggcgctga aggaccgagg cattgaatgc tttgaagctg
     5401 aggagcatac cgtgagaggt gcactacaaa ttgcaaagca aattctgtaa ctctttttag
     5461 cctttatgac gcatattgga cacatgacag acggaaccac aacaaaagaa gctgtcctac
     5521 ttgaagattc gacattcaat gtgataactc agattgaaaa gaaggccgat tttctatatt
     5581 catctgttga aaaatacatt cgtgatgcag agaaagacaa gaagcccgag cttgtcaagc
     5641 tgtggagtac tatgaaggaa gacgaacaaa agcacctaaa aatgctgcgc gaacaactag
     5701 taaaggaagt aaaagaaaac aaactacagt aggcaaaatt caatgctatg ctgtgtgtca
     5761 ggtctctgtc tatcgccagc aggtctctta atttttctga tatatgatat attctattcg
     5821 acttaggaaa tggctcgact acaacatata tgcatcttaa gtattttctc ttagagacta
     5881 aaaaacaagc acaataacta attggttaat atgacattta agttctgttg ggctaccatt
     5941 tcttttagaa tatcaatatt agtcagtgtg gttattccca cttaacttgc ttttgtcatc
     6001 acagactgga ctttttcatc caaagtatta ggcttatcca gcctttcgtc gatccttatt
     6061 gtcgagatca cgcgcttggc tcctactgat ttggcagaat tatgagcggc atgtattgcc
     6121 tgaaagatat catctagatt gtctgattct acctgggtgc tcattggagt gagagttact
     6181 tttttgagat tctttatgct acgaattgca tcataggcag ctgctatctc tttgcccata
     6241 cttgtatctg aagtaccaat tggcgcgatg ctaatctctg catttacagt tggcaactta
     6301 ctcatccact cctatattgg caagtttact aattaacagt ttatttgtat tattattctg
     6361 actttgatca gtggtggtgg tgtgttggca tcccttcttt tttctgttca cgcttcatag
     6421 cccggtacat cgcaggatat gccactgcat aacctgcggg tagtattatt ccaagaccta
     6481 gccagaataa tgcatccgtt agagcagcat gcataaaacc aggtattaca aaaacaagcg
     6541 agttctcagt atctccattg ccgcaatgct tgctgtatcg ccaaccattg tgactcttgc
     6601 tgcttgtcta aacggcattg tt
//
LOCUS       DASQTF010000148         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_230487,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000148 DASQTF010000000
VERSION     DASQTF010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230487"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VE308_03520"
     CDS             complement(<1..737)
                     /locus_tag="VE308_03520"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HZB00076.1"
                     /translation="MQDVEMELIPAIPAPEFKEITDWANSQPLSIKGLKGKVVLIDCW
                     TYTCIFCLRTIPIMRRLQQKYGKYGLQVVEAHSAEYQFATDHANIRHALSRYNIGDVP
                     VAFDINNKTWEAYGNMYWPKHVFVDHNGLIRYEHAGYGGMEDFEDVVVELLQEAGNKP
                     VGEKEHEDPKDEIYDIYGMHFYGMAPEICVGYSRLRRFGNNHTLKPDSVNVMVDPGLH
                     DVNVVYLRGSWIWQRERVQYRPGGKNQ"
     gene            complement(1044..2207)
                     /locus_tag="VE308_03525"
     CDS             complement(1044..2207)
                     /locus_tag="VE308_03525"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HZB00077.1"
                     /translation="MLALMDNWEGSSSKAGKARYIIFSACGYGNIGDDAIMLGTARYL
                     ITRKNATDLFIFSYSPNETQQLLHKAGLTSVATIRCGRFKSLIKALSSTTRRKRIMIG
                     GGTLITNRTFFTLYYLIPAFLFKLFFSRSSDVYFFGVAAEERIKRPLLKFMLSLVLRH
                     SIKKAFVRDNFTEGVLKHFANSTGTKVSTIGDPALFLKEWEDGSGANKKKDEDHRTVF
                     KHTAKILVSARDLDLCSSDYHARCFANIFDELVEVIHQKTGMAIVINFVPFCIHKTSP
                     LERDDLFGEKIKSLMKYFNYFQIEKIDNPIEIMQKFNEVDFCMCMRLHSLIFAYMTGR
                     KCLAISYSPKVFNFAKDNDLPCVNISEVESHKEKVIDIVVQSLFIDKDKTKSN"
     gene            2496..>3464
                     /locus_tag="VE308_03530"
     CDS             2496..>3464
                     /locus_tag="VE308_03530"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZB00078.1"
                     /translation="MLDHDPGITIFAKKPSKEKDTDIPISPQVKMMRNGAQVADDLRL
                     LISSSKGATYVEARYQSRVASEVNFINGELERIRVVENAGCGIRVLVDGCWGFTSTSN
                     ISNETLKESLSQAISAARILAQTKKNKVRGLVESKMVTGTFRTAANSHNLSDISIEQK
                     VQVTKEAEKEARSNRVVKTASCTYRDMLDHKVIINSDGADLEIYDSKPEFSVTAIARK
                     ASQSATAHEGVGITGGWDELFKKKSHLEYAHTASARAAKLLDARHVSGKKTTVILDPA
                     MVGLLSHEAIGHTVEADFVLSGSVVKDKLGSKVASDLVTLVDSGRSE"
BASE COUNT         1044 a          748 c          699 g          973 t
ORIGIN      
        1 ttctgattct tgcctccagg tctatactgt actctctcac gctgccatat ccagcttcca
       61 cggagatata cgacattaac atcatgcaag ccgggatcga ccatgacatt tacactatct
      121 ggcttgagcg tatggttatt accaaaccgc cggaggcgtg aatagcctac gcatatctca
      181 ggggccatcc cgtaaaagtg catgccatag atatcataaa tctcatcctt cggatcctca
      241 tgctcttttt ctccaaccgg cttgttaccg gcctcttgta gtaactcgac aacaacatct
      301 tcaaaatctt ccatgccacc gtaaccagca tgctcataac ggataagtcc attgtgatct
      361 acaaagacat gttttggcca atacatattg ccataagctt cccacgtctt gttgttaatg
      421 tcaaatgcaa ctggaacatc accaatgtta tatcgggaaa gagcgtgcct gatgttggca
      481 tggtcggtag caaactgata ttcagccgaa tgagcctcaa ccacctgtag tccatacttt
      541 ccatattttt gctgcaggcg cctcatgata ggtatagtcc gaaggcaaaa gatgcaggta
      601 tatgtccaac aatcgatgag cacgaccttg cccttcaagc ctttgatcga cagtggctga
      661 gagttcgccc agtctgttat ttccttgaat tcaggggcag gaatcgccgg gataagctcc
      721 atttctacat cctgcacagg attagacaga atataactgt agatcctaac attttcaagt
      781 gcattgtgac aaaaaatttt gctagctgtt gttgcagacc ccagaaaggc attaggactg
      841 taaattggca ggagaatcct tctttacatt ctctgacaca atggagcgta tgttctataa
      901 aacgcaacga ggaacacacg cagtgttgac tagaagaatc aaagccttac agacctacta
      961 gaagtgcctc cttcccatca gatattaaaa gagatcatta agaggaatta ccttcttctg
     1021 ctgctactgc ctctaattta atttcaatta gactttgttt tatctttatc tatgaacaat
     1081 gattgaacta cgatatcgat aaccttctct ttgtgtgact ctacttcact tatgttaaca
     1141 cacggcaagt cattatcttt ggcaaaatta aaaacctttg gagaatatga tatcgccaag
     1201 cactttcttc cagtcatgta agcaaaaatt aaagaatgaa gcctcataca catgcagaaa
     1261 tctacctcat tgaacttttg catgatctca attggattat ctattttctc aatttggaag
     1321 taattaaaat acttcattag actctttatc ttttctccaa acaagtcatc tctttctaag
     1381 ggagatgtct tatggataca gaagggaaca aaattgataa caattgccat tcccgttttt
     1441 tggtgtatga cttcaacaag ttcatcaaat atattagcaa agcatcttgc atgataatcc
     1501 gacgaacata aatctagatc cctagcacta acaagaattt ttgcagtatg tttaaacaca
     1561 gtcctgtggt cctcatcctt ttttttatta gctccactac catcctccca ttctttaagg
     1621 aaaagtgcag gatcaccaat tgtggaaacc tttgtgccag tactatttgc aaaatgtttc
     1681 aatacacctt ccgtgaagtt atctcttacg aatgcttttt ttattgaatg tcgaagaacc
     1741 aatgataaca taaacttcaa taatggtcgc tttatccttt cttcagccgc cactccaaag
     1801 aaatagacat cagatgatct ggagaagaat agcttgaaca gaaaagccgg gattaaatag
     1861 tacaaagtga agaaagtgcg attagtgata agagtgccgc cacctatcat tattcttttt
     1921 cttcttgttg ttgaggacaa agcctttatc aaactcttaa aacgcccaca gcgtattgta
     1981 gctaccgacg tcagtcctgc tttatgtagt aactgttgtg tttcgttagg cgaatatgaa
     2041 aaaatgaaca agtcggttgc attcttcctt gtaatcagat atcttgctgt tcccagcatt
     2101 attgcatcat cgccgatatt gccataacca caagccgaga atattatgta gcgggctttg
     2161 ccagccttac tacttgagcc ctcccagttg tccatcaaag ccaacatttt ttcatctgat
     2221 acgctattta cggtggtgat ggtttgtccc tacactgcca agaatttgaa tggataatga
     2281 cgttcttcta ttataagctt cataaatttt ttgttggttg atgcttagta tttgttctgt
     2341 ttagaagcag caaaacaaca tttatgccta cctatcaaaa ttggatgctc ccattcatca
     2401 tccaaaacta ataatacttt gaagttattg caattgatta caagttttct cagaaagaaa
     2461 attgaaaaaa atatgaactg gagtaacttt gtatattgct agatcatgat ccgggtataa
     2521 ccatttttgc aaaaaaacca tccaaagaaa aagatacaga tatacctata tctccgcaag
     2581 tgaaaatgat gcgaaatggt gcacaagtag ctgacgacct cagattgttg atttcatctt
     2641 caaagggcgc cacttatgtc gaagcacgct atcagtcaag ggtggcaagc gaggtcaatt
     2701 ttatcaacgg cgaacttgaa agaataagag tggtggaaaa cgctggctgc ggaattaggg
     2761 ttctggtgga tggctgctgg ggctttacca gcacaagcaa catctccaat gagaccctaa
     2821 aagaatccct tagccaggct atctctgcag cacgcatact tgcccaaact aagaagaata
     2881 aggtcagggg actcgtggaa tcgaaaatgg ttacagggac ttttaggacg gcggccaaca
     2941 gccataatct gtcagatatt agcattgagc agaaagtgca agttacaaag gaagcagaaa
     3001 aggaggctcg aagcaacaga gtcgtcaaga ccgcttcttg cacataccgg gatatgcttg
     3061 atcacaaggt catcataaac agcgatggcg ccgaccttga gatttatgat tcaaagccag
     3121 agtttagtgt aacagcaata gcaaggaagg caagtcagag tgcgacagcc catgaggggg
     3181 ttggtatcac aggcggctgg gatgaactat tcaaaaagaa gagccatcta gaatatgccc
     3241 acacggcatc ggcaagagcg gcgaagctgc tagatgctag acatgtgagc ggtaaaaaga
     3301 ccacagtgat tctggatcct gcaatggtgg ggctcctctc gcatgaggca atcggccaca
     3361 ctgtcgaggc tgactttgtg ctatctggct cggtggtaaa agacaaacta gggagcaaag
     3421 tagcaagtga tcttgtcacg ctggttgaca gcggcaggtc ggaa
//
LOCUS       DASQTF010000149         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_866372,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000149 DASQTF010000000
VERSION     DASQTF010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866372"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1851
                     /locus_tag="VE308_03535"
     CDS             136..1851
                     /locus_tag="VE308_03535"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HZB00079.1"
                     /translation="MKDNAAGNNDGRQQKTPIEETKNIAQLLVRCLENEGVKYVFGIP
                     GEENIHFIDALSGSSSSIRFILVHHEQSASFMADMYGRVTGKAGVCSATLGPGAINLL
                     LGTADAFTDSVPLIALSAQVGVDREYKETHQFVDLVSMFKPITKWAAEIGIPSAVPAM
                     IRKAFKLAQTERPGACYIAVPQDIEQMSVSADLQPLLRSDVYDSYPSPSQVLRATRIL
                     EQAKSPIILVGHGAARNDAQDALIRFSEQLHIPVATTFMGKGVFPDNHPNALGTVGFM
                     RRDYVNFGFDLADVLICVGYDMQEFDPARINPDADKKIIHVSRYAAEVDPHYPVAVGI
                     ESNISMALDELAKSVNSKGALKTESRKIQNLLREELEEGAQNDSFPVKPQRIVSDIRV
                     ALDDTDIVLVDTGALKMWMARLYPTYKPNTCLISNGLSTMAFALPGAIAVKLACPEHK
                     VLAATGDGGFLMLGSELETAVREHVPIVILIWEDQSYGLIKWKMDLELGHHSSVDFYN
                     PDFVKYAESHGAKGYKIGSPGDLLPTLKSALEDDKSVSVIACPVDYSENMKLTDKLGR
                     LTDAL"
BASE COUNT          682 a          466 c          514 g          621 t
ORIGIN      
        1 gagtcaacca ttctatatgg ttctgaaact aataacagag gaccaagcgt aataattagg
       61 gttgagtctt aaggataagt aataaaaata ataataatga tgtaattagg acagtaggtt
      121 ttgagttagc attctatgaa agacaatgca gcaggaaata atgatggaag acaacaaaaa
      181 actccgatcg aagagacaaa aaatattgcc cagcttctgg tcagatgcct cgaaaatgag
      241 ggtgtaaagt atgtcttcgg aatccctggc gaagagaata ttcattttat agatgcatta
      301 agtggttcat cttcttccat tcgcttcatc ttagtgcacc atgagcagtc tgcatcattt
      361 atggctgata tgtacggccg tgtaactgga aaagccggtg tctgtagtgc gacacttgga
      421 ccaggagcca ttaatttgct gttgggaaca gctgatgcat tcacagacag cgtccccttg
      481 attgccttga gcgcccaagt tggtgttgac cgtgagtaca aggaaacgca tcagtttgtt
      541 gatctcgtta gcatgtttaa gcccataact aagtgggcag ctgaaatagg aattccgagt
      601 gcggtacctg ccatgataag aaaagcattc aagcttgctc aaacagaacg tcctggtgct
      661 tgctatattg ctgtaccgca ggacattgag caaatgtcag tatccgcgga tcttcaacca
      721 ttgcttcgat cagatgtcta tgatagttat ccttccccat cacaagttct acgcgccaca
      781 aggatattgg aacaagcaaa gtcacctatc atcctcgtgg ggcatggtgc cgctcggaat
      841 gatgcccagg atgcgctgat tagattctct gaacagcttc acatcccggt agcaactaca
      901 ttcatgggaa agggagtctt tccagacaac catccaaatg cactaggtac tgtaggattt
      961 atgcgtcgtg attatgttaa ctttggattt gacttagccg atgtgcttat ctgtgtaggc
     1021 tacgatatgc aagagtttga cccggcgagg ataaatcctg atgcggacaa gaaaataata
     1081 catgtctctc gatatgccgc agaggtggat cctcattacc ccgttgcagt tggaattgaa
     1141 agcaatatat caatggcact tgatgagctt gccaaaagtg taaattccaa aggggcacta
     1201 aaaactgaaa gtcgaaaaat ccaaaatttg cttagagaag agcttgagga gggagcacaa
     1261 aatgactcct tcccagttaa accgcagcga atagtatcag acattcgtgt ggcacttgat
     1321 gacacagata ttgtacttgt ggacactggt gcattaaaga tgtggatggc ccgcctctac
     1381 cctacctata aacccaatac ttgcctaatt tctaatgggc tctctactat ggctttcgct
     1441 ctccccggag caatagctgt taagcttgca tgtcctgaac acaaagtact tgctgctacc
     1501 ggtgatggag gattcttgat gctgggttcg gaattagaaa cagcagtacg tgagcatgta
     1561 ccaatcgtaa tcctgatatg ggaagatcag tcatatggac ttattaaatg gaaaatggat
     1621 ttagagttgg gacaccattc ttcagttgat ttctataatc ctgattttgt gaagtatgca
     1681 gaaagtcatg gagccaaagg atataagatt ggcagccccg gcgatcttct tccaactttg
     1741 aaatctgcac tggaagatga caaatctgtt tcagtaatcg catgcccagt tgattactct
     1801 gagaacatga agctcacaga taagcttggc cgtttgacag atgctctcta gtgcaaagca
     1861 ttatttatac tcgccataag gcctcctaag aaaaagcaga actcacaatg tacaataagg
     1921 caatacactg caaaagccaa aaaataaatt gataggttta gtctcaagcg gatatgttat
     1981 agcgagtatc tgtccctgaa ccaaatctca gctataacca tgaatggtaa tcatatcaga
     2041 taataatggt tactagatgt ccagcatctt aatacagtgg atcaagggta cttcgtccaa
     2101 agataaatat tgtgagaata gtcttaaaat gggggctatg tactgcttcg ctatcgactg
     2161 gcaatgttta ggatgttgcc gtcagggtcc ttgaaccaag cgaccttcat atcgccggca
     2221 atgtggacat cgccttctct tgttacccct ggcatgtcat aatgctcgaa tatgatacct
     2281 ttg
//
LOCUS       DASQTF010000150         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_688161,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000150 DASQTF010000000
VERSION     DASQTF010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688161"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            787..876
                     /locus_tag="VE308_03540"
     tRNA            join(787..825,842..876)
                     /locus_tag="VE308_03540"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:822..824,aa:Gly,seq:tcc)
     gene            complement(896..1459)
                     /locus_tag="VE308_03545"
     CDS             complement(896..1459)
                     /locus_tag="VE308_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00080.1"
                     /translation="MSKIASFSRKVLISSVKQLAPYLIIGPRRLVLIGAIAAVALAII
                     FYPLIVQTPFDPERVAIQLAGVELLSGSEGEQRLDLRVAFQITNTNEFTLTTSRIVYD
                     LFADGAPVGSDTLSYEDVPVNGRPAFFSNEPVTISDTMTLQYTDEMAGLFSRILNNST
                     DINWRVSGSATIESGTTLQEKRFSDEL"
     gene            2027..2521
                     /locus_tag="VE308_03550"
     CDS             2027..2521
                     /locus_tag="VE308_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZB00081.1"
                     /translation="MAVADGIVMWVHLVGASIWVGGSIFLGIVLVPVLKSYTKSLEEM
                     VALMVKIGRRFNKVTVPAFAALIASGIYNSRAFLGEPSTVLDTTYGIILLTKVILVIG
                     TIVAYIVHIRLLDADMERKIMSGQGGNIYVQSVRTKIIILGEIIVVFSVAILLLAALL
                     DSGI"
     gene            complement(2518..3564)
                     /gene="rtcA"
                     /locus_tag="VE308_03555"
     CDS             complement(2518..3564)
                     /gene="rtcA"
                     /locus_tag="VE308_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HZB00082.1"
                     /translation="MTLSPHLVKIDGSQGEGGGQILRTAISLSAVTGKPIEVNNIRAK
                     RANPGLRPQHMAGIRIIADLFHAKFENLKVGAEWIRFSPSDKFEGGSVKFDIGTAGSI
                     PLTLMTVVSAVSLSNNSLQIEITGGTDVKASPTIDYIKNVVAKSYLSIGAKFTVDVLK
                     RGYYPKGGGVVQSTIMPCKKPGTIELLATGYLEPKIISVCGQLPVHIAKRQISSALIA
                     LEKTDIRCSNYTASLESSISPGSSILVYSASDFGLYVGGDSIGELGKRAEAVGTEAAK
                     RFLESILAQATVDPFLADMIVLPLALSKGRSKYRIARVTQHLLTNLHVVSEIVGCKYS
                     IDQHGGSYVVMIEG"
BASE COUNT         1108 a          873 c          836 g         1186 t
ORIGIN      
        1 ggccaaaaac aaggcagtcg atatgctgct taatattata tttccagaat aagatttcta
       61 ttgttcctta acaatatctc tcaccacaat cggccacgat ggtctagttt gctacaattc
      121 cgagtacatc ctatatctat acattaagac aagatttgtc aagactagaa tttttagctc
      181 ttcttgctat tcagagcgca cttgaagaag ccctatctcg ataacgaatg ctattttgag
      241 caatatcaaa acaggacggg caacctctga tgctacggtg acggaaatta tttcgaagca
      301 atagcatgtc gccttagaga ccaaggaatg tttgtatcaa cagtaatcag ataagagcaa
      361 ccgagctgca attttggcat ctctatgcag ttgtattgaa aaatttacat ctgagctaaa
      421 ctcattgtca aatgttgcag tgacgatgca ctccttattg actaatggtt tgagcttaga
      481 tagactactc aatttgggat gagttgtgtt ttatcaaatt ataaaaatac ctgcctcaat
      541 ggttctaatc gtggtatcga taataattca gaatcgtctt ttgcataaca tatcatgatc
      601 cccaaaatac caataacttg gacggcaggt acagcagaca ggattgagat cctacgtatc
      661 actattctta ttatctgtac attttcattg gtcgacatat ttgcattggc ttgaaaactc
      721 aacaggccca aaaagtagca gctttgaata tactatatat tgggttgcat gatcgtagga
      781 cagagagcgg tcatcgtcta gtttggttag gacactggcc ttccatgcaa aatgcgaagt
      841 tagcccgtaa cccgggttca aatcccggtg gccgcataat tcttttcttc ttttatcata
      901 attcatcaga gaatctcttt tcctgcagtg tcgtgccaga ttcgattgta gcagagccac
      961 ttacgcgcca attgatatca gtgctgttat tcagtatcct gctgaagaga cctgccatct
     1021 catcagtgta ttgcaacgtc atcgtgtcgg atatagtaac tggttcgttt gaaaagaaag
     1081 caggcctacc gttaacggga acatcttcat atgataacgt atctgaccct acaggagcac
     1141 catctgcaaa taggtcgtat acgatccttg aagtcgttag ggtgaattca ttagtgtttg
     1201 ttatctgaaa tgccactctg agatcaagcc tttgttcacc ttcgctccct gacaagagtt
     1261 cgacccctgc cagttggatt gcgactctct ctgggtcaaa tggcgtttgt acaataagcg
     1321 ggtagaaaat gattgcaagg gcaactgcag caatggctcc aataagcaca agccttcgcg
     1381 ggcctattat caagtacggt gctagttgtt taacagagct gattaaaacc tttctggaaa
     1441 aagacgctat tttgctcatt tcatattgct gggcttataa tgtagagaaa cagcatccaa
     1501 ttaatccggc taattcagat ttgtgacaac taaaaggtgt ccagtttagg tgagaatata
     1561 tctttctgtc acctattgtt tagattttcc catccatatg ctgtaattta ttgcggggta
     1621 ttaatcccga gaaaaccgaa ggaggatgtt gaccaacaac aaccatcgag acagatattt
     1681 ctttatagaa gtcatccgct atgtctggtt ttatgtgcct ccttcatgtc ttggtcacat
     1741 ttccagcatt ctcctacaat actgataaca aattctctgc atcctgtact agatcggaac
     1801 taggctctac caaatcttgc ccgttctagg ctacaacaaa tatgacatcc cacaacatgc
     1861 tttaaggatt cttcttcttc ataatcggtt atgaccaata tcgttctttg ttcgtagact
     1921 gataaaattt gagtcccttg tagcaaatcc ctaaaatccg ccgacaatgg attttctgcc
     1981 ttaaatgcag catcaataat atattacgaa aatgtgaaaa atcatcatgg ctgtagcaga
     2041 cggcatcgtc atgtgggtgc atctagtagg tgcatcaata tgggtgggag gctcaatctt
     2101 tctcggaatc gtgcttgtcc cagttctcaa gtcatatact aagtcgcttg aggagatggt
     2161 tgcattgatg gtcaagatcg gcaggcgatt caacaaggta actgtacctg catttgcagc
     2221 cttaatagca agcggcatat acaactcgcg cgcctttctt ggggagccta gcactgtact
     2281 tgatacgact tatggcatta tcttacttac aaaagtcatt ctagtcatag gaaccattgt
     2341 agcatatatt gtacatatca ggctactgga tgctgatatg gagcgcaaga taatgtcagg
     2401 acaaggggga aacatctacg tacaatcagt acgaactaag ataatcattc taggtgagat
     2461 cattgtcgtc ttctcagttg caatactgct cttggcagca ctccttgata gtggcatcta
     2521 gccttctatc atcactacat agcttccgcc gtgctgatct atactgtatt tgcatccgac
     2581 aatctcgctt acaacatgca agtttgtgag caaatgttga gttacacgcg cgattcgata
     2641 ctttgatctg cccttactca atgcaagtgg aagaacgatc atgtctgcaa ggaaggggtc
     2701 tacggtagcc tgtgcaagta tgctttccaa aaaccgcttg gcagcctctg tcccaacggc
     2761 ctctgctcgt tttccaagct ctcctatgga atcgccaccg acgtatagac caaagtcgga
     2821 cgcagagtat acaaggattg aagatcctgg agaaatagaa ctttctaatg acgccgtgta
     2881 gttactacat cggatatccg ttttctctag agctatcagg gcagaagata tctgtcgctt
     2941 ggctatatgt accggcagct gaccgcagac actgataatc tttggctcaa gataccctgt
     3001 agccaggagt tcaattgtgc caggcttttt gcagggcatg atggtggact gcactacgcc
     3061 acctcccttt ggatagtatc cccgcttcag tacatcgact gtgaactttg ctcctatgct
     3121 taggtaactt ttcgcaacaa cattcttgat ataatcaatt gtgggacttg ctttgacgtc
     3181 tgttccccct gtgatctcaa tttgcaggct gttgttagac agcgagactg ccgacactac
     3241 agtcatcaaa gttagtggga tgctgccggc cgtgccaata tcgaacttta ctgagccgcc
     3301 ctcgaatttg tcagagggtg agaacctaat ccattctgca ccgaccttca ggttttcaaa
     3361 ttttgcatga aaaagatcag caataatcct gattcctgcc atgtgctgcg ggcgcaggcc
     3421 ggggtttgcc ctctttgctc taatgttgtt aacttcaatc ggcttgccgg ttactgcaga
     3481 gagcgaaatt gcagtgcgca atatctgccc gccgccctcc ccctgagatc catctatctt
     3541 gactaagtgg ggagacaatg tcaatttgta ggtaccagtt ctaatttagc ttaaaaatat
     3601 atatgcagtc cttatctgac ggtaaaggct caattaatca aaactctctc tgtattactt
     3661 ttcagttact gccctaagaa tgccatagaa ttttatgtac tcagtcaaga gtgaggctca
     3721 gaagagagcc atgggattgc tgcgttttag aaaatgtgaa ggtgaggggc actgcagtag
     3781 cattgaaggt tcgctgttat gctattggca aaatcaatga ctagatagag tatttactcg
     3841 aatttcgatg atggaagcga aggcttgtaa agacaaacaa catcggctta tcgcatatga
     3901 gttgacctag tttttcgcaa atatattgag gtcttagcaa cagttccgca atagtacaaa
     3961 aaactactta catctctctt gatctacata gtcactgttt aga
//
LOCUS       DASQTF010000151         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_512130,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000151 DASQTF010000000
VERSION     DASQTF010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512130"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..268)
                     /locus_tag="VE308_03560"
     CDS             complement(83..268)
                     /locus_tag="VE308_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00083.1"
                     /translation="MEKDQELQKNNQKLVDQERTLNPQDICGGDIARRFEFLSSYHGA
                     NESTYASRKLDNDLSMP"
     gene            complement(333..1562)
                     /locus_tag="VE308_03565"
     CDS             complement(333..1562)
                     /locus_tag="VE308_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZB00084.1"
                     /translation="MSGKMSNATDNTANSKDDIASLDVNKTSRNTQLPSRSFHVEQLE
                     DKDRYMTLSIGPQHPGSGHMRIIVVVDGDIIVSADPDVGYVHRGEEKMSENRTFVQNV
                     PHIERPVIHDSSNILYSYCLAVEELIGLQAPERAMYLRAILAEIDRIQYTLYWLAILG
                     IFIGHSTMFMWATADRELFVELADMASGNRITHSYIVPGGVRNDLPEGFADKALKSLD
                     YFESKRLPEYDKIFYDNPLFRQRSEGVGVLSKADAISLGVTGSVLRASGIDYDVRKRE
                     PYDIYSDIQFEVPISKTGDSFARSILPVYDIRQSINIIKQCLTKMPQSGQIRVKLQPN
                     PRGPPAEAYRRVESGRGALGHYIVSDGTPKPYRHKMSVPSVRNLAAMSHLLKGARLAD
                     LPAIYWSLNIWPVEIER"
     gene            complement(1739..>2630)
                     /locus_tag="VE308_03570"
     CDS             complement(1739..>2630)
                     /locus_tag="VE308_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00085.1"
                     /translation="KYLLRHMAFAESMLTLEYLALVREDELTANYRFGVNFEDLKWLA
                     SLALQQIAGGHGLSSPDILYPSSPSPTLTPGTSQPHKTVNSPPMANTERTADLIKDLS
                     LSIFSQEEIELNPGGGTSRSYDDFVRPRSRDYGIHRPNFPHRVPPTMYSFRKPDDGEW
                     KRIIKEFFDEQKCITCNAEVLPQQFDIWIGEPLKDNATPGSKRDQRMSEVLTDIDSVS
                     ANANRGGNRDDAVTVESADERQIIDLDTNVTDRELLTYNYGIIPMDGRDAFIAFAKPK
                     LPCPHCGANPAGSVSLMPKY"
BASE COUNT          646 a          611 c          574 g          799 t
ORIGIN      
        1 gtcattgcgt ggatactcct ctgcatggtc tttaagcatt agtcatagaa cctagggaat
       61 tccatgttct atgattattc tgttaaggca tacttaagtc gttgtctagc tttcttgaag
      121 catatgtact ctcattagca ccatggtaag aggataaaaa ttcaaacctc ctggctatat
      181 ctcctccgca tatatcttga gggtttaaag tcctctcctg gtcaacgagc ttttgattgt
      241 ttttctgcag ttcttggtcc ttctccacgt gctatctttc atttattata tgttaaaagg
      301 attggaggag gcaatgatag aggtggatct atctatcttt caatctcgac tggccagatg
      361 ttaaggctcc agtagattgc tgggagatct gcaagcctgg cgcctttcaa gagatgcgac
      421 attgctgcca gattcctgac ggaaggcaca ctcatcttgt gcctgtaagg tttgggtgta
      481 ccatcactta caatatagtg cccaagggcg ccccttcctg attcaacccg gcgataggct
      541 tcagctggcg ggcctctagg attaggctga agcttgaccc taatttgacc tgactgcggc
      601 atcttggtca agcattgctt gatgatattg atactttgtc ggatgtcata cactggaaga
      661 atactccgtg caaaggaatc gcctgttttt gatattggta cttcaaactg aatgtcgcta
      721 tagatgtcgt atggttctct ctttcttaca tcatagtcta tgccacttgc tcggagaact
      781 gatccagtta cacctagact tattgcatct gccttagaaa gcacacccac tccttcgctt
      841 ctttggcgaa ataggggatt gtcataaaat attttgtcat attctggtag acgtttactt
      901 tcaaaataat ctagggactt taatgccttg tcggcaaacc cctcaggcag atcgtttcgc
      961 acgcctccag gaactatata cgaatgcgtt attctattgc cactcgccat atctgccaac
     1021 tcaacaaaga gttccctgtc cgcagtagcc cacataaaca tagtcgagtg gccaataaat
     1081 atgcccagga ttgcaagcca gtacaatgta tactggattc tgtctatctc tgccaatatc
     1141 gctcttagat acatggctct ttccggggct tgcagcccaa tcaattcttc aacagcaaga
     1201 caatacgagt aaagtatgtt acttgagtca tgtattaccg gccgttcgat atgcggtaca
     1261 ttttggacaa acgtcctatt ttcagacatc ttttcttcac ctctgtggac atagcccacg
     1321 tctgggtcgg cgctaacgat aatgtcgcca tccactacta ctataatcct catgtggccg
     1381 gagccggggt gctgcggtcc aatgctaagc gtcatgtacc tgtctttgtc ttcgagctgc
     1441 tcaacatgga agcttcgtga agggagttgg gtgttcctag acgtcttgtt aacgtcaagg
     1501 gatgcaatgt cgtctttact gttagcagtg ttatcggtag cattagacat ttttcctgac
     1561 atatgccatt ttttcagcat ctcttaaatt ggttggttga gagaataact gtacttagtt
     1621 ctgaaacctt tcaaagcaat cagcaaacta atgctaactg atgctttaac cttgtttacg
     1681 gttagccgcg tcaactgtct gtctcaaatg cgcaatgaat atgcccagga ggctactact
     1741 aatattttgg cataagagag acagacccgg cagggtttgc cccgcagtgt gggcagggaa
     1801 gctttggctt tgcaaatgca atgaaggcat ctctaccatc catcggaatt attccatagt
     1861 tataggtcaa aagctccctg tctgtaacat tcgtgtctaa gtctatgatt tgccgctcat
     1921 ccgcagactc aacagtaact gcatcatccc ggttaccgcc cctattcgca ttcgcactta
     1981 cactatcaat atctgtgaga acttcgctca tcctttgatc tctttttgaa cctggcgtag
     2041 cgttatcttt cagtggttcg ccaatccaga tatcaaactg ttgaggcaga acctctgcat
     2101 tgcaggtaat acacttctgc tcgtcaaaaa attcctttat gattctcttc cattcgccgt
     2161 cgtcgggttt tctaaagctg tacattgtag ggggaactcg atgagggaaa tttggcctat
     2221 ggattccata atctctgctt cttggtcgca cgaaatcgtc atagcttctg ctggttccac
     2281 caccagggtt aagctcaatt tcctcttgag aaaagatgct cagagacaag tcttttatca
     2341 agtcagcagt cctctctgta ttggccatgg gcggcgaatt gacggtcttg tgtggctggg
     2401 atgttcccgg tgtcagggta ggactaggag atgaggggta aaggatgtca ggtgatgata
     2461 agccatggcc tcctgcaatc tgctgcaatg ctaaagaagc aagccatttc aagtcctcaa
     2521 aatttactcc gaagcgatag tttgcagtca attcgtcttc tcgcacaagc gcgagatact
     2581 ccagagtgag catagattca gcaaatgcca tgtgccttag aagatatttt
//
LOCUS       DASQTF010000152         4454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_233497,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000152 DASQTF010000000
VERSION     DASQTF010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233497"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VE308_03575"
     CDS             complement(<1..210)
                     /locus_tag="VE308_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZB00086.1"
                     /translation="MAAAIKVFAVDIDGTLTENGGGTVHLAALAKLRYLEKMGYNVIY
                     VTGRSSIEAYVLAVFGGTTRIAIGEN"
     gene            complement(285..416)
                     /locus_tag="VE308_03580"
     CDS             complement(285..416)
                     /locus_tag="VE308_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00087.1"
                     /translation="MYYLTSVIVGSAILAGLFVSIAIRRPRAIIALNNNWQKIEVRA"
     gene            complement(424..567)
                     /locus_tag="VE308_03585"
     CDS             complement(424..567)
                     /locus_tag="VE308_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00088.1"
                     /translation="MMVDLINSLMLVALGFAPTFVAMEAAWRMGKIIGKRGEEKTLTW
                     RSA"
     gene            complement(809..1324)
                     /locus_tag="VE308_03590"
     CDS             complement(809..1324)
                     /locus_tag="VE308_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00089.1"
                     /translation="MYAKLCNIEVLPSYIKEVAVGDILTVQALITNALDWHHFYEKVS
                     TIKIGDKTDFTVITQEGERVTGFGNIVEVDKWSNHGHYGFKIKISVKRQKSLIDRRIK
                     APSKVKLPSMQLESVSDAAESASVIVPPAHLLTAAAPPVTTSTTTVITAHDINSFQEM
                     LKESLTMDITS"
     gene            complement(1454..1822)
                     /locus_tag="VE308_03595"
     CDS             complement(1454..1822)
                     /locus_tag="VE308_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00090.1"
                     /translation="MIAQEQDSPIIASKVKYLGSSLLPSPIELEATIVLYRNRMHIPE
                     LDAIIPIDKVSQIELAKGNDLPSQVTVMFGVVGLIVEKENPYMMIKIANSPDSLLFKF
                     ENMVLADKLVKEIKVARDLQ"
     gene            complement(1935..2279)
                     /locus_tag="VE308_03600"
     CDS             complement(1935..2279)
                     /locus_tag="VE308_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00091.1"
                     /translation="MITTANYQDAVFTPYQSNSEVAIQKMIDASQRMLEMNSQGEEGS
                     AGGGMSPSLKRHLRLQIKTLENAPRDADKLRRLLQVNQRQKEEAMDIEDTQRLVTEIE
                     MLKVCIVFGEHR"
     gene            2323..2586
                     /locus_tag="VE308_03605"
     CDS             2323..2586
                     /locus_tag="VE308_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00092.1"
                     /translation="MSQEEPPIEIVINGVIGWDERSITTNQPMQPEIGNLSKGKTYFP
                     KVSRATLKNTSRREHYLTNIQIGNIRQSHGSRTTTYEYVKTAP"
     gene            2988..3084
                     /locus_tag="VE308_03610"
     tRNA            join(2988..3018,3041..3084)
                     /locus_tag="VE308_03610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3045..3047,aa:Arg,seq:ccg)
     gene            complement(3181..3768)
                     /locus_tag="VE308_03615"
     CDS             complement(3181..3768)
                     /locus_tag="VE308_03615"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZB00093.1"
                     /translation="MSFTELLRKEQILSFLRPALVDRPYLPTDAALATPAAVLVIIHY
                     HKDRPHIFLTKRSSNLKSHTGEISFPGGRYVESDGTFLATALRETAEEVGIAFSIDQI
                     VGCLNAVRTMTSNHFIVPFITIQDMLPQYRISTSEVEAVVDVPLIETLKSIELDSEHY
                     HLAKDAFKFTYGNNMIWGATARVLKQMHDILIRPS"
     gene            3830..>4454
                     /locus_tag="VE308_03620"
     CDS             3830..>4454
                     /locus_tag="VE308_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00094.1"
                     /translation="MFFRIVLPSDAINVAYELNPPKIVKGERFDLGQLKDNIQEMINR
                     ALQLRGLVSALHLTDSVLGIPRLSSITAARYIKEHIGGEVRLSCSIRVRDRNFPSLCQ
                     TVSDAILIGAESVLILMGDEPADKLGDSGLRPSTAVRMLKKEGYGLNIKLDLSFPAKV
                     KDKLAQSIKNKLDVTPHSLVTQSISSLSDLGEIADLAKPYGIKVTAVL"
BASE COUNT         1258 a          948 c          934 g         1314 t
ORIGIN      
        1 attttcccca attgcaattc tagtcgtgcc accaaagact gcaagaacat atgcttctat
       61 tgatgacctg cctgtaacat agattacatt gtagcccatt ttttcaagat agcgcagctt
      121 ggcaagggct gcaagatgca ctgtgccgcc cccgttttca gtaagagtgc cgtcaatatc
      181 tactgcgaaa accttaatcg ctgctgccaa gggagaaatt aaaccacaat cttaatgtta
      241 taaaagtgta gtgagaaaaa gtatagtgag agatgaattt tgggttatgc cctgacctct
      301 atcttttgcc agttgttgtt taatgcaatg atagcacggg gcctgcgaat ggcgatggaa
      361 acaaatagcc cggcgagaat tgcagagccg actatcactg atgttagata atacaatagc
      421 atttcacgct gacctccacg tgagtgtttt ttcttctccc cgcttgccaa ttatcttgcc
      481 cattctccag gcagcttcca ttgcaacaaa tgtaggggca aatcctagcg ctacaagcat
      541 taatgagttg atcaagtcga ccatcattag ttgaatacaa ggaaagggat tttaattcta
      601 cttgatcagg cttttgatat atcatatggt aaagtatgtt atcaggagca taactgatct
      661 atcataagcg attttaagaa cctaaatttt cattcccgca atatatggga ttatctagag
      721 acagacgaga ggggacatgt cgaagaactg tacaaaaata gtgcaagcac cctttgcaga
      781 ttaaagtagt ggcaggtaaa acaggcagct aggaagttat atccatcgta agcgattctt
      841 tcagcatctc ttggaaggaa tttatgtcgt gtgccgttat tacagtagtg gtgctggtag
      901 ttacaggagg agcagcagcc gtcaagaggt gagcaggcgg aacaataact gacgctgact
      961 cagcagcatc tgacacactt tcaagttgca ttgatggcag ttttactttg ctaggagctt
     1021 ttattcttct atcaatcagc gatttctgcc ttttgacact tatcttaatc ttaaagccgt
     1081 agtggccgtg attgctccat ttgtcaactt caacaatgtt gccgaagcca gtcacccttt
     1141 cgccttcctg agtaatcact gtaaaatcag ttttatctcc aatcttaatc gtagatactt
     1201 tttcataaaa gtgatgccag tcaagcgcat ttgtgattag tgcctgcacc gtcagaatgt
     1261 ctcccaccgc tacttccttg atgtagcttg gcagaacttc aatgttgcac agctttgcat
     1321 acatatacac aaaattaacg cacatctaga ataaataact tctgaaaagc taaacgaata
     1381 tccaaatgaa atttgttaga gttatggatt tgtatatatt gtacaataat tctgttataa
     1441 tagtataata tttctattgc aaatctcggg ctactttaat ctcttttact aacttgtctg
     1501 caagtaccat attctcaaac ttgaatagca ggctgtcagg gctattggca attttgatca
     1561 tcatataggg attttccttc tctactatta gaccgactac tccgaacatc acagtgactt
     1621 gcgatgggag gtcgtttcct ttagctaatt caatttgaga tactttatcg ataggaatta
     1681 tggcatcaag ctcgggaata tgcattctat tgcgatagag cacaatcgtg gcttctagtt
     1741 ctattggaga gggtaagaga gaggagccaa gatatttcac ctttgacgca attattgggc
     1801 tatcctgctc ttgagctatc atgtcgataa tagcatgaca tgatagataa tcctgtcact
     1861 ttattagcac tcgcttgtaa tattagtgcg atcacagagg aggaataaga cgaaatttgc
     1921 aaccacacct tcttttatct gtgctcacca aatacaatac aaaccttaag catttcaatt
     1981 tcggtaacta acctctgagt atcttcaatg tccatagctt cttccttttg tctttgattt
     2041 acttgcaaaa gccgtcttaa cttatccgca tctcttggag cattctcaag agtctttatt
     2101 tgtaatcgta agtgtctctt cagagaagga ctcattcctc ctcctgctga tccttcttct
     2161 ccttgactgt tcatctctaa cattctttga cttgcatcaa tcattttttg tatggctact
     2221 tcactgttgg actggtaggg ggtaaatact gcatcctgat aatttgcagt agtgatcact
     2281 ttttaagttg caaacggtca taaaatctca aggataataa caatgagcca agaagaacca
     2341 ccaattgaaa tcgttataaa tggagtaata ggttgggatg agcgttcgat taccaccaat
     2401 caacccatgc agcctgaaat agggaatctt tccaaaggaa aaacatattt cccaaaagtt
     2461 tcgcgtgcaa ctttaaagaa taccagcaga agagagcatt atctcacaaa tatccaaata
     2521 ggaaatattc gtcaaagcca cgggtcgaga accaccactt atgaatacgt aaagactgca
     2581 ccataactga cttgcactaa caaaactatg ttgtccggag aggtatatta gcctcaagcc
     2641 ttttcctaat ttctgctcct cgataccaaa tatagtgcaa cttttagcat ttcgatttca
     2701 gtaactaatc tttctgtatc ttctatctgc tcagtttctc ccttttgtct ttgatttact
     2761 tgcaaaagcc gtctctgctt tctctagata ttctctagtt ttttgacctg cttctctgat
     2821 catacaagtg catgtggctt gacataggat tggatacaga agagaaagca tgaagagagt
     2881 cagcccagat acctgatgag cttaagcagg cagtgaaaag ctactaaaga atatcgtcct
     2941 ttgtggtggg tatgtttcat atatgagaag atttaaacac gcagacaggg cccgtcgccc
     3001 agtcaggata gggcgctgtt cctttagaaa agaagcgcaa gcctccgaag ccagttgtcg
     3061 tgggatcgaa gcccaccggg cccgctagcc ctataggcta caatgcaatt tgatgttcag
     3121 atgaagaaga agaagagtta gtatatacat atacatgtaa gcgagatgca atgaatgagg
     3181 tcatgatggt ctgatgagaa tatcatgcat ctgctttagc acccttgcag ttgctcccca
     3241 tatcatgttg ttgccataag tgaacttaaa tgcatctttg gcaaggtgat aatgttcact
     3301 atctagctca atgcttttga gtgtttcaat gagtggtaca tcaaccactg cttccacttc
     3361 gcttgttgaa atcctgtatt gaggcaacat gtcctgtatt gttataaatg gtacaataaa
     3421 gtggtttgat gtcatcgtac gtactgcatt caagcaacca actatctggt caatactgaa
     3481 tgcgattccg acttcttctg ctgtttcccg caaggcggta gcgaggaacg tgccgtcgct
     3541 ttcgacatat ctacccccag ggaaagatat ttctcccgta tgtgacttca ggtttgaact
     3601 ccgctttgtc agaaagatat gcggtctgtc tttgtgatag tgtattatca caagtactgc
     3661 agcaggtgtt gctaatgctg catcggttgg caggtacggc ctatctacaa gggcgggtct
     3721 aagaaatgag agaatttgtt ccttcctcaa cagctcagtg aatgacacta gggtcaatat
     3781 atgctctgga tgctcttgcc ggcgacctcg catttaataa ggcctaagga tgtttttcag
     3841 gattgtattg cctagcgacg caataaatgt agcctatgag ctgaatccgc ctaagatagt
     3901 gaaaggagaa aggtttgatc tcggacagct aaaagacaac atacaggaga tgataaaccg
     3961 cgctttgcag ctccgcgggc tcgtaagtgc gctccacttg acggactcgg tgcttggtat
     4021 tccccgccta tcgagcatca ctgcagccag atacatcaaa gaacatatcg gtggagaagt
     4081 aaggctgagc tgcagtatca gggtgcgcga tcgcaatttc ccttccctat gtcagacagt
     4141 atcagatgca attcttatcg gggctgagag tgtgcttatc ttgatgggcg atgagccggc
     4201 agacaagcta ggcgattctg gtctgcggcc aagcaccgca gtcagaatgc ttaagaagga
     4261 gggttacggc ttgaatatca aactcgatct ttcatttcca gcaaaggtca aagataaatt
     4321 ggctcagtca ataaagaata agcttgacgt tactccacac tctcttgtaa ctcaatcgat
     4381 ctcctcgctt tccgatttgg gcgagatagc agaccttgcc aagccctatg gcatcaaggt
     4441 caccgcggtg ctga
//
LOCUS       DASQTF010000153         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1162586,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000153 DASQTF010000000
VERSION     DASQTF010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162586"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VE308_03625"
     CDS             <1..196
                     /locus_tag="VE308_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00095.1"
                     /translation="HEADGGIIALLKPYQTRPASHSTKKSQGFCIICGEMATTEALFK
                     LEGAMIIQRYCEKCLPGARY"
     gene            1105..1398
                     /locus_tag="VE308_03630"
     CDS             1105..1398
                     /locus_tag="VE308_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00096.1"
                     /translation="MATIRLYKRKPTFVELSSLVEELSNTGLPDTLKKYYNEPAEHEA
                     YSIVAGPTFIQYLNKLFKAQIAAGDILQFESDHQNKFFMFSLTGTWDEIIKLQ"
     gene            2005..2445
                     /locus_tag="VE308_03635"
     CDS             2005..2445
                     /locus_tag="VE308_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZB00097.1"
                     /translation="MSTNLETIDVTENAQEAAKKMNDKRISSVLVVDRDKKSKEPIGI
                     VTERDLVIRVCAAGTSSKEVSIQEIMSSPIATVEPQATVETAADLMLSNKVRHLLVVD
                     EQTRMPVGIIAPSDLNKYLRANIDMDEVNARILEAIKAEELTER"
     gene            complement(2658..3686)
                     /locus_tag="VE308_03640"
     CDS             complement(2658..3686)
                     /locus_tag="VE308_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00098.1"
                     /translation="MTAILAGMQDSLLVLNSSKAGWKVHESLTGTHPQSIASDPVNPS
                     TAYCGTFGAGVWKTDDGGQHWDRIGKGFISSPNVTSVSVSPLERGKNGGFNVVYAGTE
                     PSEFYRSDDGGESWERMISLNNLSSSQSWSFPPRPWTHHVRWIEPDINSPGYVFVAIE
                     AGALVQSRDGGRTWIDRVKRGPFDTHTLATHSKAPKRLYSSAGDGYFESFDYGETWKR
                     PIAGLKHQYLYGLGVDSQDPQTLIVSASSGAWQAHNTEDAMSFIYRRAEDAGEWELVS
                     KGLPESNGTIISILVASSKNAGEFYALSNRGIFCSTDSGISWKALDISWPKEYYSQHP
                     WALAIEEE"
     gene            4061..>4363
                     /locus_tag="VE308_03645"
     CDS             4061..>4363
                     /locus_tag="VE308_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00099.1"
                     /translation="MEDYNERRLLATTVEALDVDVLKAGHHGSRTSTGNAFLNAVSPE
                     AVVISAGANNTYGHPHPEALDRINNAGVANVHRTDIDGTIVITTTGDGNYTIEAMGS"
BASE COUNT         1351 a          918 c          883 g         1211 t
ORIGIN      
        1 ccatgaagca gatggaggta tcatagcatt gctaaagccg tatcagacaa gacctgcatc
       61 acacagtacg aaaaagtcac aggggttctg cattatttgt ggagaaatgg cgacaactga
      121 agcactattc aagttagaag gcgcgatgat tatccaaaga tattgtgaga agtgtttgcc
      181 aggcgctcgg tactaattct cgtactagga agaagggctc cgtgaactaa aaagaaacag
      241 gctagaggga tagaagttcc ttttttaata ttgaaattat ttgcgaatag tccaacagat
      301 agaaaagcaa aatactgttc tcttatgacg tctatgctac tctcaagaca tggttaattg
      361 agacccctgt atcactctca ccgaaaaata atgtaacatg tttgcacaga agctgatgca
      421 gtaagaggat atagcactgt ttataccaac agctcagttg ttatactgcc ataattgtag
      481 gtcagtcagg cagtcattat gcaactcata atatagctgt ccacactgtg cgcttaccgc
      541 ttagagtgct ctttataacc ccgatcgtat tgttgcaaaa gactttcttt gataccctct
      601 attaggtttc gtagctcgcc attatcattg agttcgttta gcttcattga gtggaatcct
      661 aacgattcaa gctctcctat caaataagac tgttcgtgag ctctagtttg tagaaaaaat
      721 tttgacattg aatagataag ttgaactagt atatacatag aacggtaaag gtttgatgcg
      781 taggccggtt gacctatgat catcataatt atgaagtttc ttccgaattt ttcattttac
      841 agctgaatta aggtaagtct tggaacaatc atctgagcaa aactctaaaa cgcctatggc
      901 agagcttgaa aataatacac cctcggtaag gttttgaaat tctttttgcc atatgattta
      961 catactcttt tcattttatt gttctgacaa tatcctcatt tagcacatca gcattgaggt
     1021 tctttgcatt gcgctgcaga gaaatagaca gggctatata ttggcgtacg gacatgatag
     1081 agatcaagaa gatgtgtatt ttcattggcg acaattagac tgtacaagag aaaaccaaca
     1141 tttgtggaat tatccagttt agtagaagag ctcagcaata ctggattgcc tgatactttg
     1201 aagaaatact acaacgagcc tgctgaacac gaagcataca gtatagttgc cggacccacc
     1261 ttcatacagt acttgaacaa attattcaag gctcagatag cagctggtga tatactacag
     1321 tttgaatcag atcatcagaa taaattcttc atgttctcat tgaccgggac atgggacgag
     1381 ataatcaagc tgcaatgact aaccctgatt tccccgtagg tgatatataa cattaagcag
     1441 gcatgccaca gatggtaaaa aacaatgtaa agcaatgcgc acatatcctg gcataatatc
     1501 tatagatttc aaatggctgc ataccgagcc aattacctta tttgcacttt tagccaacta
     1561 atccacatac gacgaccaaa gttgtactta gtcggacttc gcccgttgca tgacggatag
     1621 atctttaagg cactagcaat caaaccctct attacataga gagacctaat ggcactggag
     1681 agatgaactt gaaagaaaat agagaaaatt caaaagaatg cctcattcag ttcttaaaat
     1741 atacttatcc taaataatca aaattaattc catttagatt actttatttt tctctatctg
     1801 ttgttattaa aatatttttg actcaatgat atctccaagt tgtgctccaa gatatttttt
     1861 gttccgtctc gaaaggcagg ttaaactcct tattccgcta aatcttgtca tgatacgatt
     1921 gttcacagac acatagatta tcttaatatt tttattattc atttaaccac aagtatattc
     1981 atgcaaaatg cagtcaagga aattatgtcc acaaacttgg aaacgataga tgtaacagaa
     2041 aacgctcaag aggctgccaa gaagatgaat gacaaacgga ttagttcagt tctagtcgtt
     2101 gacagggaca agaagagcaa agagccaata gggatagtta ctgagaggga tctcgtaatc
     2161 cgagtctgcg ccgcaggaac aagcagcaaa gaggtaagca tccaagaaat aatgtcctcg
     2221 cccattgcaa ctgtagaacc ccaggcaaca gttgaaactg cagcagatct gatgttgagc
     2281 aataaagtca ggcacctgct agtagtagac gaacaaacaa gaatgcctgt aggaataatt
     2341 gcaccaagtg atctgaacaa gtatttgcga gctaatatcg acatggacga agtcaatgcg
     2401 aggatcttag aggctatcaa ggctgaagaa ttgaccgagc gatagacagg atcatattat
     2461 caaaatattt ctcttgcaac agacatacaa taattttttc tctatgacac cacaaaatct
     2521 tgatgtgaag tgtaagattt gaagaggata ttataaagaa tgcccaaatc ctcaacccat
     2581 agattttact taggtaattg gcaaacttct tttgccgaaa tgtaattttc tatggctaaa
     2641 atcaggattg tactaggcta ttcctcttcg attgctagtg cccaagggtg ttgtgaataa
     2701 tactccttcg gccatgagat atctagcgct ttccacgata tgcctgaatc agtagagcaa
     2761 aatattccgc ggttgctaag agcatagaac tctccggcat tctttgaact tgcgaccagg
     2821 attgagataa ttgttccgtt tgattcagga agaccctttg aaaccagttc ccactctccg
     2881 gcgtcttcag ctcttctgta tatgaatgac atggcatctt ctgtgttatg cgcttgccaa
     2941 gctcctgaag acgcagatac gatcagagtt tggggatctt gagaatctac acccagtcca
     3001 taaagatatt gatgcttaag tccagctatt ggtctcttcc atgtttctcc ataatcaaag
     3061 ctctcaaaat agccgtcacc ggctgaagag tacagccgtt ttggagcctt gctatgcgta
     3121 gccagagtat gagtatcaaa tgggcctcgc ttaactctgt ctatccaagt cctgccgcca
     3181 tcacgacttt gaactagtgc tcctgcctct atcgcaacaa aaacataacc tggactattg
     3241 atatccggtt caatccaacg cacatgatga gtccagggtc ttggaggaaa gctccatgac
     3301 tgagacgagc tcaggttgtt taggctaatc attctttccc aagattcccc tccatcatct
     3361 gatctataaa actcgctagg ctcagttccc gcatacacca cgttgaatcc gccattcttt
     3421 cccctttcta gaggactaac tgaaaccgag gtcacattcg gactggagat gaaacccttt
     3481 ccaattctat cccaatgttg tccaccatca tcagttttcc atacgcctgc accgaatgtt
     3541 ccgcaatagg cggtacttgg attaacagga tcagatgcta tgctctgtgg atgagttccc
     3601 gtcaaagact catgaacttt ccatccagcc tttgaagaat tgagtacaag aagcgaatcc
     3661 tgcattccgg ctagaatggc cgtcatgatc tatacagatc tatccaacat ctctcttaag
     3721 atcgtttagt tttaagccaa agctataccc gtgtcctctc gagagacaaa tatatggtgc
     3781 aataatagaa cttttttgat ttattgcaga ataagccaca catttcgtat ggttgacaaa
     3841 ggcgtcagtc tgaacagtca gttatcatgg caacaagtat tattagtgac acaggcttct
     3901 tctatgagtt aggaaatggc aagcaacaat gataatcttt tggaacaggt aatcgaagcg
     3961 gaagctccca aaaggcgtaa acaatgaaga gggttttaat gacaattcgg ttgtactcaa
     4021 actgacatat ggcgagttca cggcgctgtt tactggcgac atggaggatt acaatgaaag
     4081 aaggctcctt gcaacaaccg tggaagccct tgacgttgac gtattaaaag ccggtcacca
     4141 tggcagccgc acgtcgaccg gcaatgcatt tctaaatgct gtttcgcccg aggcagtcgt
     4201 catctcagcg ggagcgaaca atacctacgg ccacccacat ccagaagctc ttgacaggat
     4261 aaataatgct ggcgtagcca acgtgcatcg cacagatatt gatggaacga tagtcattac
     4321 cacgaccggc gacggcaact atacgattga agcaatggga agc
//
LOCUS       DASQTF010000154         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_320282,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000154 DASQTF010000000
VERSION     DASQTF010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320282"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VE308_03650"
     CDS             <1..767
                     /locus_tag="VE308_03650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZB00100.1"
                     /translation="FNNIMLFDVHIHLTDKEYSGYVHHIISSLSMMKIIACSVTVDVE
                     TTIRSFNLFNTHRNIVKQFIGIHPEASAREDLDTFREIFRSNLQSIDGVGEIGLDGTY
                     NVPIKRQEQVFSAMLELAELTGRPVSIHSRRMLDKVLEILPSYHIKSALLHWFDGSKK
                     QLAKCMEIGLYVSYGPPLVYSEDKKVLLKNTDTDRFLVETDGPVQYSRCFENKPSNST
                     SCLVSVVASAAHIIGISYDETVEILQKNSKAYLAYT"
     gene            806..1039
                     /locus_tag="VE308_03655"
     CDS             806..1039
                     /locus_tag="VE308_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HZB00101.1"
                     /translation="MNRIKRISTELLERYPDKFGLDFNANKNFISEVAIIRSKVLRNE
                     LAGYITSHLRKQAAQEKASMTSAVGETEEEVEE"
     gene            1036..1800
                     /gene="cgi121"
                     /locus_tag="VE308_03660"
     CDS             1036..1800
                     /gene="cgi121"
                     /locus_tag="VE308_03660"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HZB00102.1"
                     /translation="MITIRLLGGVKKAVGKPVVNLDRPSASVSDILDFLTTISAEPRL
                     LHRSNLIITLNDVDSASLQGHNTMVKSGDTVKIVTVVHGGMDYMLNGYHVSITGVETI
                     MDDPGKLVDILRAQHSNVSIQGVNPNAVYGEEHVLGVLRIVLEAEKRKIMLTKRCETE
                     LLIRLTGTNQIAEGIRRAGLKKDTAGCFIAFSQDSKLLRQFENQLKSQFHLNDSVLKP
                     SEDKRNRLASMFGFATKLDDGEFLQYLLEKAAILIR"
     gene            complement(1806..>2189)
                     /locus_tag="VE308_03665"
     CDS             complement(1806..>2189)
                     /locus_tag="VE308_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HZB00103.1"
                     /translation="LVQSSLDPIAVIDKLKDLVASEPWQIRYILRVLPVQVVIPTRLD
                     TIRQAARELITKIGNDTFRVTVEKRHNSLGSMQIVKAIADQISNRVDLENPGWVILVQ
                     VLGGRTGVSVLRPNQIFSSVVEKRK"
BASE COUNT          679 a          445 c          486 g          579 t
ORIGIN      
        1 ctttcaacaa tatcatgctc tttgatgttc atattcatct cactgacaaa gaatattcag
       61 gctatgtgca tcatataata tcgagcctga gtatgatgaa aataatcgcg tgttctgtta
      121 cagttgatgt cgaaactaca attagaagtt tcaacctttt caatactcat cgcaacatag
      181 tcaagcaatt tattgggata catccagaag cttcggctag ggaagatcta gatacattca
      241 gagaaatctt caggtcaaac ttacaatcaa ttgacggtgt cggcgaaatt ggtcttgacg
      301 gcacatacaa tgtgcctatc aagcggcaag agcaagtctt tagtgcaatg cttgagctgg
      361 cagagttaac gggcagacct gtatcgattc attctagaag aatgctggat aaagtactag
      421 aaatactacc gtcatatcat atcaaaagcg cattgttgca ctggtttgat ggcagcaaga
      481 aacaattggc aaaatgtatg gaaatcggac tgtacgtatc atacggtccc ccgcttgtat
      541 actcagaaga caagaaggtg ctcttgaaaa atacagacac agatcgtttc ttggtggaaa
      601 ctgatggtcc agtgcaatac tcgaggtgtt ttgagaacaa gccatccaac tccacttctt
      661 gtctagtcag tgttgttgca tcagctgcac atatcatagg tatatcctat gatgagacgg
      721 tagagattct gcaaaagaat tctaaggcct atcttgcata tacataacaa agataaaata
      781 tccatgaaat tgccttctct actacatgaa cagaataaag cgcatatcta ctgagctgtt
      841 ggagaggtat ccagataaat ttgggcttga ttttaatgct aataagaact tcatcagcga
      901 agtagccata atcaggtcaa aggtcttaag gaatgagcta gccggttaca tcacctcgca
      961 tttgcgaaag caagccgcac aagagaaggc ttcaatgacg tctgcggtcg gagaaactga
     1021 ggaagaagtt gaggaatgat aactataaga ctgctgggtg gagtgaaaaa agcagtcggc
     1081 aaacccgtgg tcaatcttga ccggcccagt gcgtcggtct cagacatttt ggacttttta
     1141 acaaccatct cagctgagcc ccgtctcttg catagaagca atttgataat tacattaaat
     1201 gatgtcgatt ctgcttcact tcaggggcat aataccatgg taaaaagcgg tgatacagtt
     1261 aagatcgtca cggtggttca cggtggaatg gattacatgc ttaatggcta tcatgtatcg
     1321 attacaggtg tcgagacgat catggatgat cccggcaaac tggtcgatat actacgtgcc
     1381 caacacagca atgtatcaat acagggagta aatccaaacg cagtctatgg agaagaacat
     1441 gtgctagggg tacttagaat agtacttgaa gcagaaaaaa gaaaaatcat gcttacaaag
     1501 aggtgtgaaa cggaactact aatccggctc acaggtacca accaaattgc agaagggatc
     1561 agaagggctg gtttgaagaa ggataccgcc ggctgcttta ttgccttttc acaagatagc
     1621 aaattgttac gccaatttga aaaccagctt aaaagccagt ttcacttaaa tgattctgtg
     1681 ctgaagccaa gtgaggataa gaggaaccgg cttgcaagca tgtttggttt tgccacaaaa
     1741 ttggatgacg gtgaatttct acaatacctc ttggaaaagg cagctatatt gataagataa
     1801 aaatgtcact tacgcttttc aactacagag ctgaaaatct gattgggccg gaggaccgat
     1861 actcccgtcc gccctccaag tacctgtact agaataaccc agccagggtt ctccagatcg
     1921 accctgttac tgatctgatc tgctatcgcc tttacaattt gcatagaacc aagagagttg
     1981 tgtctttttt caaccgttac cctgaaagtg tcattgccga tttttgtgat gagctctctt
     2041 gccgcctgtc ttattgtgtc aagccgtgtg gggataacaa cttgtactgg caatacacgc
     2101 agaatataac gaatctgcca aggttctgag gccacaagat cctttagttt atcaatcaca
     2161 gcaatggggt caagcgagct ctgcacaag
//
LOCUS       DASQTF010000155         3879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_55251,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000155 DASQTF010000000
VERSION     DASQTF010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55251"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VE308_03670"
     CDS             <1..706
                     /locus_tag="VE308_03670"
                     /inference="COORDINATES: protein motif:HMM:NF017013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HZB00104.1"
                     /translation="VIGDDLGMIASGPTCPDNSTFNNALNILKKYRIESPVTAIRYVA
                     KGAKGRIEETPKPQEELFSRIHNVLIGNNAMACKAAVSHLKKHGVQAVNLGSEFNGEA
                     KEFGAFLARRALGLGRKRLAIVAGGETIVKLKMSKNGLGGRNQEAALSYLAELDRYDV
                     AMACIGTDGIDGNSDAAGALVSPKTKMLAKKMDLQKYLNMHDSYRAFKKLNSLIITGF
                     TGTNVNDIAVICPELK"
     gene            complement(1030..2022)
                     /locus_tag="VE308_03675"
     CDS             complement(1030..2022)
                     /locus_tag="VE308_03675"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZB00105.1"
                     /translation="MPSEEPEEHLLCMLRLQKGYISGKKLAKEIGISTSGISKRITIL
                     RQYGYNIESNHRMGYRLAGETDLPLPWELAKVLQTSFVGKDKIIYRQTTHSTQNLAIL
                     LAEKDPSLDGIVIIAEQQTNGRGRENRRWLSPKGGIWLSVVLRPRISASMITLLPFAG
                     ALAVCDAIKKTTLLDAKLRWPNDVTINGKKVAGILIDISMEGERINYAVIGIGINANV
                     DSSSISSGLEKSIKVTSLSDELTHKTSTLGLTKMILERLEYYYIEMKHCVPHTIIEEW
                     KQNSDIIHQKVAVIQNNRTIQGIAADVNDDGSLLVRTDDCDNINVVASDIHVRY"
     gene            2313..2633
                     /locus_tag="VE308_03680"
     CDS             2313..2633
                     /locus_tag="VE308_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00106.1"
                     /translation="MVHQMYKYVIGVPNAILLHKHPGRSRPCVMHPWGDPKWYLHSRM
                     RTVYVRGSVKDKESKKKNNRSMIPIGYYCADCKRFLTTEEYKQKSNERWEDWQAAHPS
                     KKKE"
     gene            3055..3282
                     /locus_tag="VE308_03685"
     CDS             3055..3282
                     /locus_tag="VE308_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZB00107.1"
                     /translation="MTYRSRTDIISEILEAANGGITQTKIMYKALLNYAQMKQYLMVL
                     THNGLLSYELETRTFKTTEKGLRFLYTYNHV"
BASE COUNT         1156 a          823 c          784 g         1116 t
ORIGIN      
        1 cgtgatagga gatgaccttg gaatgattgc ttctgggcct acctgtcctg ataactcgac
       61 ttttaacaac gccttgaata tcttaaaaaa atatcgcata gaaagtcccg ttactgctat
      121 tcgatacgtt gccaaaggag ctaagggcag gattgaggaa acacccaagc cgcaagagga
      181 gctattttct cgcatccaca atgtgcttat tggaaataat gcaatggcat gcaaagcagc
      241 agtcagccac ctcaaaaagc acggcgtgca agcagtaaat cttggatcag aatttaatgg
      301 cgaggccaaa gaatttggag cgtttctggc gcggcgtgca ctaggccttg gacgcaagcg
      361 tcttgcaata gtggccggag gtgaaaccat tgtcaagctg aaaatgagta aaaatggcct
      421 gggcgggagg aaccaagaag ctgcactatc atatttggct gagcttgatc gatacgatgt
      481 tgcaatggca tgcataggaa ctgacggaat cgacgggaac tctgacgccg ctggcgcact
      541 cgtctcgcca aagacaaaaa tgcttgccaa aaagatggac ctccagaaat atcttaacat
      601 gcatgatagt tatcgcgcct tcaaaaagtt gaattcgctc atcatcactg gttttactgg
      661 caccaatgtc aacgatattg cagttatatg tcccgaatta aagtaagatt ttgtctcatg
      721 ttttgcagca gtggcaataa cagcaggccc tcagcgattc ctcttttcac aatctagcaa
      781 gcaagcactc tgagcctgag ggggataaag atatagagcc tgtttctgaa ctctgcagaa
      841 aaaagaattg cagagttaaa accctaaaaa tgaaaaaata gtcttaaaga tggaagaagc
      901 aggagaactg acaatactgc accaaaatac aaatactaga agaaaaaaga atattgccaa
      961 actaggatct tgacggttga cggcacacgt acctaaatga ttgacgcaaa gtgaattctt
     1021 gagacttccc tagtatctga catgtatgtc gcttgctacc acattgatat tatcacagtc
     1081 gtcggttcta actaaaagcg atccatcatc gttcacatct gcagctatgc cttgaatggt
     1141 tctattgttc tgtatcactg caaccttctg atgtattata tcagaatttt gtttccactc
     1201 ttctatgatc gtgtgaggaa cgcagtgctt catctctata taataatatt caagtctctc
     1261 aagtatcatc ttcgtcaaac caagtgtgct agttttatgt gttagttcat cgcttaatga
     1321 agtaaccttg atgctctttt caagacctga agagatcgac gacgaatcaa catttgcatt
     1381 aatcccaata ccgatgaccg catagttgat ccgctccccc tccatactga tgtcaatgag
     1441 aataccagcc acctttttgc catttattgt gacatcattt ggccatctta atttggcatc
     1501 aagtagtgta gtttttttta tcgcgtcaca tactgcgagc gcgccagcaa acggaaggag
     1561 tgtgatcatg gaagcagaaa ttcttggtct caaaacgact gatagccata tgcctccctt
     1621 gggtgatagc cacctccggt tttccctgcc acgtccgttc gtttgctgct ctgcaataat
     1681 cactatacca tccaaactgg ggtctttctc agcgagcaat attgcaaggt tctgagtgga
     1741 atgagtagtc tgtcgataga ttattttatc cttacctaca aatgaagtct ggagcacttt
     1801 tgccagctcc catgggaggg gaaggtccgt ctcaccggca agtctataac ccatcctgtg
     1861 gttcgactca atattataac catattggcg taggatcgtt atccgcttag agattccgct
     1921 cgttgaaatc cctatctcct ttgccagttt cttccctgat atatatccct tttgcaatct
     1981 gagcatacag aggaggtgct cttctggctc ttctgacggc attttggcat tagtgcgcat
     2041 tgggaagtat aattaggata tgataattgc attaatttat tccccctttt tatgcccctt
     2101 acatctcact tagtcactgt gtttattgtc aatcatacta tggcgggttg tggctgatag
     2161 ctaagcgcta gaggttatga aatgttaagt attattattg acgtttagct acagttctct
     2221 caataacaca gacctgccgg tcattgctaa cttgctgatt gctagacgac agtaggatga
     2281 tctctgctgg tataccgatc cagctcgatt aattggtaca ccaaatgtat aaatacgtca
     2341 ttggtgtacc aaatgccata ttgcttcaca aacatcctgg tagaagtagg ccctgcgtaa
     2401 tgcacccctg gggtgatcct aagtggtatc tccacagcag gatgagaact gtctatgtgc
     2461 gtggttcagt caaagataag gagtcaaaga agaaaaacaa cagaagtatg ataccaatcg
     2521 gttattactg tgcagactgc aagagattcc tgaccacaga agagtataaa caaaaaagca
     2581 acgaacgatg ggaggattgg caagccgcgc atccaagtaa gaaaaaggag tagtagtgct
     2641 agaataatcg actgccaacc tacgtgacgt gaattactca ctctcagcga ttttgttgaa
     2701 agtaatttat ggcaacaagc tacgttattt ctgataatat gatcatcttt gaatttgtta
     2761 ttctgtgatt cttgtcctta tgagctttca gagctttctt tgagtgaaag aattggccac
     2821 agatactgca tcttgaaatt atctttgtca ttacctttca ttctttgacc tcacttcgcc
     2881 cgctaaatac tgctatgact cattgacaaa agcccatcat aataaaaata cttttagaac
     2941 aatactcaac agttagcact agtttgatac acctttttca gaacttgcat aaaccatttt
     3001 tgtaacattc ttttataatg ctatttaata ccataatcca gtgcagcaaa agatatgaca
     3061 tacagaagca gaaccgacat aatcagcgag attttagagg ctgcaaatgg tggtatcact
     3121 caaactaaga taatgtacaa agcacttctt aactatgccc aaatgaaaca gtatctgatg
     3181 gttctaactc acaatggttt gcttagctat gagctagaaa ctcgaacatt caagactacc
     3241 gaaaaaggtc taaggtttct ttacacctac aatcatgtat gatatgataa aggcgacata
     3301 ataacaacaa atctggatgt aaagacgtct gaaagggaaa ataatgtgga ttttacctgc
     3361 ttactttttc ttcttttttt gctttactta gaaactttgg aattctggtg gctttaactg
     3421 tcgtggtgag gcaacagcaa tacaacttct atagtttgtt ggaaaccttc tactttggcc
     3481 tctatctgct caaccctttg atgcatttga tcagctgttt gtaatggtga aacaacatag
     3541 acaccagtat cggggggtct tgcataccaa acagaataga agctggcaga aatctaaatc
     3601 tagggatgtc aaacatcggc tcatcaaact tctttctgcc tgattaatcc aaagcattgg
     3661 gaaagaggtt ccagtcacat gcttctaatt cgtgtgcaat tctaaatgaa gatattgtcc
     3721 atgcatccat catttcaacc gccatcccaa tccgattatg atttctaatg gctaaagggt
     3781 accataaata atactcggtt ctggaacgtt atcaacacat aaaaaagccg atgagtcgac
     3841 tgagaggagc aatcttggat cctacgtcct tccaaccat
//
LOCUS       DASQTF010000156         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_321918,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000156 DASQTF010000000
VERSION     DASQTF010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321918"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VE308_03690"
     CDS             complement(<1..437)
                     /locus_tag="VE308_03690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZB00108.1"
                     /translation="MLDNAMELALTITNLVLAAIMAGIFGVWVYFLTYMTKSFRQSPM
                     LESFDRTSISRFPKVSVILPARNEGRYIGRCLDSLLEQDYPNFEIIAINDSSTDRTGQ
                     IIKAYAANDLRVIHIDALPKPEGWTGKNWACHQGYLGARGELL"
     gene            complement(452..880)
                     /locus_tag="VE308_03695"
     CDS             complement(452..880)
                     /locus_tag="VE308_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZB00109.1"
                     /translation="MIKVMITVNASREMSAPIDRIWNIVADVDNEPKYWRGTKTVKNI
                     SKADNKIEREVTIAFKDSKCRQTVLLNPRKSVEIMITEGPIRGTKRVLLHPLDNHNTR
                     IDVVWNIKLAGFLALFIGMVKRHIAEGTDEALVRIAKAVE"
     gene            complement(1275..2000)
                     /locus_tag="VE308_03700"
     CDS             complement(1275..2000)
                     /locus_tag="VE308_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HZB00110.1"
                     /translation="MKTIAFIVITLLAGAIAGTILGVVNQLAVEPYIEHAIELEMQNT
                     AQSGQVINPAEFTAYRFWQKGGEIAAGTILGLSIGSLYGIVFAYTRGMVPGSNNKKKA
                     LIVAGIMWLVLFLMPALKYPANPPAVGNPETIYYRQSLYIAFLAISGFSALGLAFLYP
                     KVTSLNTNKAIIPSAYAAIMAGAYLAMPANPDSINAPIDLVIGFRITSTITISMFWGL
                     LGIIFGTFWDKLNPDETTRISVR"
     gene            complement(2016..2243)
                     /locus_tag="VE308_03705"
     CDS             complement(2016..2243)
                     /locus_tag="VE308_03705"
                     /inference="COORDINATES: protein motif:HMM:NF021038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HZB00111.1"
                     /translation="MLVRNQITKTSAGVPRIAVGILLGVLAFGAFIVGFDQGEILGFT
                     VPADGSGREPGTNWAHEFYHDMRHAAGFPCH"
     gene            2355..2990
                     /locus_tag="VE308_03710"
     CDS             2355..2990
                     /locus_tag="VE308_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4443 domain-containing protein"
                     /protein_id="HZB00112.1"
                     /translation="MILEKVASRYAPSRSLSFDVVHVFAALQRIGNKGITSRNALCSG
                     LVLGEGTVKTLVKHLKMHGLIETSNKGTKMTAKGKGIYEGLLSAIPAEMSLPKCSVAL
                     SRYNYAVLVREFGFAVGLGIEQRDAAIKVGATGATTLLFKDNKFVMPTSNNDSLKKER
                     EVRRMLVEKLDPREDDVIIIGSADNNEKTAEIGAKNAALLTIFSHEKHHSH"
     gene            3527..3976
                     /locus_tag="VE308_03715"
     CDS             3527..3976
                     /locus_tag="VE308_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZB00113.1"
                     /translation="MILVCVDGSESSMKAADYAIELAKKHEAQLISLYVVVSQLGYAY
                     SSGAFGLVTPSSINELLDKSKQEARKWFDEIGKKAMAQGVTVKVEMVASPTSIVPAIV
                     DYANKNKVDLIVTGTRGRRRSGLAKLLLGSVASGVVTYANCPVMVVK"
BASE COUNT         1125 a          906 c          927 g         1167 t
ORIGIN      
        1 atcaagagtt cgccccgagc ccccaaatat ccttgatgac atgcccagtt cttgccagtc
       61 catccctcag gtttaggtaa tgcatcgata tgtatgactc gcaagtcatt agctgcataa
      121 gcctttatta tttgaccggt cctatcggtt gacgaatcgt tgattgcaat tatttcaaag
      181 tttggataat cctgctcaag caggctgtca aggcaacgtc caatataacg cccttcattt
      241 ctagcaggca ggatcacgct tactttgggg aagcggctta tgcttgtccg atcaaacgat
      301 tcaagcattg gagattgccg aaatgacttg gtcatgtatg tgagaaagta cacccaaacg
      361 ccaaatatgc cggccattat ggcagctaat accaagttcg ttattgtaag ggcaagctcc
      421 attgcattat ctagcaagaa ataggggcta tttattcgac tgctttcgca atgcggacaa
      481 gggcttcatc agtgccttcc gcaatgtgtc ttttcaccat acctatgaac aaagccagga
      541 atccggccaa tttgatgttc cataccacat cgatccttgt attgtgattg tctaaagggt
      601 ggagcaggac tctcttggta cccctaatcg gaccctctgt tatcattatt tcaactgatt
      661 ttcttgggtt gagcaggact gtctgacggc attttgagtc tttaaacgcg atagtaacct
      721 cgcgctcgat cttgttgtct gccttactga tatttttcac ggttttggtg ccgcgccagt
      781 acttaggctc attgtcaacg tctgcaacaa tattccatat ccggtcaata ggagcagaca
      841 tttcccggct tgcattgacg gttatcatta ccttaatcaa taatgcgttg taataatgta
      901 taataatatt gtaactttta tgaaagttcg tttgtgggtg gaagatttca gttgaagggg
      961 aaaagcagtg gccagcgcca cagccaaaaa cattgattat aatgattatg gccctaactt
     1021 tgagcgtaaa ctttaccttg caatgagact atgtaacaat tttctttcca gcccaagttg
     1081 agcttgcatt tacaagaccc taggctactg catcttacgc tagcagggtt cagaatatct
     1141 ctctgtatct agaatacgag ccagtgtctg ctgtagacat gtttcttctt tctgctgtca
     1201 ccgtgacttg aattactgcg gattctttgg cttccaaacc ttccagctga tctgtagata
     1261 ttattacatt atttttaacg aacgcttatt cgcgtcgtct catcaggatt gagcttgtcc
     1321 caaaaggtgc caaaaatgat gccaagcaga ccccagaaca tactgatggt gattgtgctt
     1381 gttattctaa agccgatgac caggtctatt ggtgcattta ttgagtcagg gtttgcgggc
     1441 attgcaagat atgctcctgc cattatagcg gcatatgctg agggaattat cgccttgttt
     1501 gtattcaaag atgtcacctt cggatacaaa aatgcaagtc cgagcgcgct aaatccggat
     1561 atcgccaaaa atgcaatgta gagactctgt cggtaataga ttgtttcagg atttcctact
     1621 gcaggggggt ttgcaggata tttaagcgca ggcatcaaaa ataataccaa ccacatgatg
     1681 ccggcaacaa tgagtgcctt cttcttgtta ttagagcctg gaaccatgcc acgggtatac
     1741 gcaaagacta ttccgtacaa cgatcctatc gataatccca gaatggtgcc ggcggctatt
     1801 tcacctccct tttgccagaa ccgataggct gtaaattcgg cagggttgat tacttggccg
     1861 ctttgagcgg tgttctgcat ttcaagttcg attgcgtgct cgatatacgg ctcaactgca
     1921 agctggttca cgacacccaa gatagttcct gcaatcgctc cagcaaggag agtaataaca
     1981 ataaaagcga tagttttcat aaaggaccaa gaaggttagt ggcacggaaa tcctgctgca
     2041 tgcctcatgt catggtagaa ttcatgggcc caatttgtcc ctggctcgcg tccactgcca
     2101 tcagctggca cggtaaagcc aagtatttcg ccctgatcga atccaactat aaatgcccca
     2161 aaagcaagca ctcctagaag aataccgact gcaatcctcg gaacgcctgc agatgttttt
     2221 gttatctggt tgcgtactaa catttacctc ggatacaggc ggctgttata taaagaatcg
     2281 gatagtttat ccaactcgta cataataaat aatgcacaca ggctctatta ctgtcatgta
     2341 tgcatatgta tgtcatgata cttgaaaagg ttgcaagcag gtatgcgcca agcaggtcgc
     2401 tttccttcga tgttgtacat gtgtttgcag ctttgcagag gatagggaac aagggcataa
     2461 cgagcaggaa tgcactgtgt agtgggctgg ttctcggcga aggtacagtc aaaacgcttg
     2521 taaaacacct aaagatgcat ggccttatcg agacttcaaa caagggcaca aagatgactg
     2581 ccaaaggaaa gggcatatac gaaggactgc tatcagcaat tccggcagaa atgagcttgc
     2641 ccaagtgctc agtagccctt agcaggtaca actatgcggt gcttgtcagg gagtttggtt
     2701 ttgcagtagg attaggcata gagcaacggg atgctgccat aaaagtgggt gcaacaggtg
     2761 ccaccactct gcttttcaaa gacaacaaat ttgttatgcc gacaagcaac aacgattcac
     2821 taaagaaaga gcgagaggta cgcaggatgc tggtcgaaaa actagatcca agagaggatg
     2881 atgtaataat aatagggagc gcagataaca atgaaaagac tgccgagatc ggtgccaaaa
     2941 atgcagcgct actaacaata tttagtcatg agaagcatca tagtcattga acttgacgcc
     3001 tgtggtatct tgttatggat atagagagac ctttgcgggt tagtccagag tcctctagcg
     3061 acagctgtag tagagggggt tatgattact cctcaagcag tatgtggatg agatacatat
     3121 ctatctcgac cttgtttctt ctctacttca caagatcggg ctcctttcgt cttccttcta
     3181 accacgttgc cacagcctgc gtgcaggcat atatagaacc atcaaaataa atctggcagt
     3241 aataatctgg tggtaaccta gttcttctat gcgggctacc ttgtgactgc atcaggagga
     3301 aaatttctat tatgcacttt catatcatca acgttgtaaa acttcttgtt gcatatacta
     3361 caaatttttt cctgctgcgt cgaccgcaaa ggtagtcgac aagtcttatg agatatatgc
     3421 aaatctcaca ttgatgttac tgcacgtcat tttttgaaag taaaccatac tttttttcaa
     3481 tccttatatc tttcgtgatg cacaacctta gctgttggct tttgcaatga tacttgtgtg
     3541 cgtggacggt tcagagtctt cgatgaaagc ggccgactat gctatcgagt tggcaaaaaa
     3601 acatgaggcc cagcttatct cattatatgt tgtcgtgtca cagcttggct acgcatattc
     3661 atctggcgct tttggacttg tcacaccaag ctcaataaat gagcttttgg acaagtcaaa
     3721 gcaagaagca cgcaagtggt ttgacgagat tggaaaaaaa gccatggcgc aaggagtcac
     3781 tgtcaaggtc gaaatggtgg ctagccccac ttctatagta cctgcaatag tagactacgc
     3841 taataaaaat aaagtcgatc tgatagtaac tgggacaagg ggacggcgcc ggtcgggctt
     3901 ggccaaactc ctacttggca gcgttgcatc aggcgtagtg acttatgcta attgccctgt
     3961 catggtggta aagtaactaa ttaccacact actactactt tatcactcgt cttcccttta
     4021 tcctatcatc atgagtgttt ggcccagcat cattaggcac aggaactgaa ggtttgctta
     4081 cctttctagt gggcttgggt gtttcatcca actctagatc cttgg
//
LOCUS       DASQTF010000157         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1348174,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000157 DASQTF010000000
VERSION     DASQTF010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348174"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VE308_03720"
     CDS             complement(<1..431)
                     /locus_tag="VE308_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HZB00114.1"
                     /translation="MVATIGTKAPNLEVTTWIQGKPTNIDKERGNVVLVEVFQVNCPG
                     CFLYSMPEAIDIYTKYRDKGLTVLGLATAFEDFDKNNLANLQKLVSTGEVIGETYRAF
                     SNSGQLRDDNKLPYKIPFPVAMDMLVKHSGPLTDSKIMDFI"
     gene            complement(472..1209)
                     /gene="psmA"
                     /locus_tag="VE308_03725"
     CDS             complement(472..1209)
                     /gene="psmA"
                     /locus_tag="VE308_03725"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:NF003075.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HZB00115.1"
                     /translation="MLPAAAGYDRAITVFSPDGRLYQVEYAIETVRRGTLAIGVKSTD
                     GVILAVEEKTRKLQISNVTQKIFQVDDHIGVAAAGYIPDARTQVDHARFFAQSNRLIY
                     DEPVDVEGVAKNLADMAQQFTQYAGVRPFGVALILAGVDKNGSALYLTDPSGTYIGYD
                     AVAIGAGSDQVTEFLEKSYKSEIGLDEGAALAIESIYMVSEEKTSTRHLKMAIIDTKA
                     KTMRKAEEEEIERYAATARDRALKRGA"
     gene            1304..1738
                     /locus_tag="VE308_03730"
     CDS             1304..1738
                     /locus_tag="VE308_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HZB00116.1"
                     /translation="MAGTPMVQDEVTFYGHPHIQSLHAKTIEITKDEHLTPKGDCIIG
                     VKANKACADLDESLKHRLKSNTAVVRIEVMIGDDSFLITGRGDERLSLLNVQDIVIRR
                     TNFVCPRTMSVLCDSASSDVPRKLVRMLQNPEAKGIFRITLE"
     gene            complement(1739..>2014)
                     /locus_tag="VE308_03735"
     CDS             complement(1739..>2014)
                     /locus_tag="VE308_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00117.1"
                     /translation="TLRAFSYNLPNLEKHSKLIEETSSRLLKVLKSVLEDEKFVTEIN
                     GRETCNLCKGDHYKEYVAFEILNAALARILQTKEYSIMVGGKKQPKK"
BASE COUNT          495 a          469 c          437 g          613 t
ORIGIN      
        1 tcgataaaat ccatgatttt gctgtcggtc agcggaccag aatgtttgac tagcatgtcc
       61 atagcaacag gaaaaggtat cttgtagggt agcttgttgt catccctcag ctgcccactg
      121 ttactgaatg cacggtatgt ctctcctatg acttcgccag tactgactaa cttttgaagg
      181 ttcgcaagat tgttcttgtc aaaatcctca aaggctgttg caagacccag aaccgtgaga
      241 cccttatctc tatattttgt gtaaatatca attgcttcag gcatgctgta aaggaagcag
      301 ccagggcaat tgacctgaaa tacttcaact agcacgacgt tgcctctttc cttgtcgatg
      361 tttgtcggtt ttccctgtat ccaggtcgtg acctccaggt ttggagcttt tgtgccaatt
      421 gtcgctacca ttgttaattc agtttagcac aatctgtata aattggtttt atcatgcgcc
      481 gcgctttaac gccctatctc tagccgtcgc agcatatctt tcgatttctt cctcttctgc
      541 ctttcgcatt gtcttggcct tggtgtcgat tatcgccatc ttcaagtggc gagtgcttgt
      601 cttttcttca ctaaccatat atatcgattc aatagcaagg gcagcgccct catcaaggcc
      661 aatttccgac ttgtaacttt tttctaggaa ttcagtcacc tggtcgctcc ctgcaccgat
      721 cgcaacggcg tcatagccga tgtatgtgcc acttgggtct gtcagataaa gagcagaacc
      781 gttcttatca acacctgcaa gtatcaaagc aacgccaaat ggtctaacac ctgcgtactg
      841 tgtaaattgc tgagccatgt ctgcgaggtt ctttgcaacg ccttctacgt ctacgggctc
      901 atcatatatc agcctgttgc tctgtgcaaa gaaccttgca tggtcgacct gagtgcgcgc
      961 gtcaggaatg tatcccgctg cggcgacgcc gatgtgatcg tcgacttgaa agatcttttg
     1021 ggtaacattt gatatctgga gctttcttgt tttttcctct actgcaagga ttactccgtc
     1081 tgtgctctta acgcctattg caagtgttcc gcgtctgaca gtttcaattg catattctac
     1141 ctggtaaagc cttccgtctg gagaaaatac tgtaatcgct cggtcatagc ctgctgctgc
     1201 tggtaacaat tcgatctaat ttttttgtaa cgttccattt aagcctacct atgagagaac
     1261 ggtctaaagc aaatgcattc ttgcatgatt ttaataatac atcatggcag gaactccaat
     1321 ggttcaggat gaggtgacct tttacggcca cccacatatt caatcgcttc acgccaagac
     1381 tattgaaata accaaggatg agcacttgac acccaaaggt gactgtataa taggggtaaa
     1441 agccaacaag gcctgcgctg atttagatga atctttgaag cacaggttaa aatcaaacac
     1501 agcagtagtc agaatagagg taatgattgg agatgattcc ttccttataa cgggcagggg
     1561 cgacgagcga ctcagtttgt tgaatgtaca ggacatagtg atacgtagga caaatttcgt
     1621 gtgcccccga accatgtctg ttctatgtga cagcgcctct tcagacgtgc caaggaaatt
     1681 ggtaaggatg ttgcagaacc ccgaggcaaa ggggatattc aggatcacac tcgagtgatc
     1741 actttttggg ctgctttttt cctccgacca ttattgaata ctctttagtt tggagtattc
     1801 tagccaaagc agcgtttagt atctcaaatg caacgtattc cttgtagtgg tcgcctttgc
     1861 aaaggttgca ggtctctctt ccattaatct ctgtaacaaa tttttcgtct tcaagcacgc
     1921 tttttagaac tttcagcaac cttgagctgg tctcttctat cagcttagaa tgcttttcaa
     1981 ggtttgggag attatagcta aaggcacgca gagt
//
LOCUS       DASQTF010000158         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1240842,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000158 DASQTF010000000
VERSION     DASQTF010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240842"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1561
                     /locus_tag="VE308_03740"
     CDS             113..1561
                     /locus_tag="VE308_03740"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZB00118.1"
                     /translation="MEALVHENCGVIGIFSLDGHNVIPFVIDSLRALQHRGQEAWGIA
                     VPGKPPFRKLGLVSSAASDFPMVINKYKSHTAIGHVRYSTLGKSTIENAQPLKVKDLC
                     VAHNGTIANVEELSGMVGGCTFTPQTMSDTLVAAQRLVMHLADKEDMAEAMHILKGEM
                     VGSFCFTFLTDEGSVYAARDTRGFRPLVLGYHRETNTYIVASESCALAAVGAQLTRDV
                     EPGELLKISKAGLASQHFANERPHAHCAFEYTYFAHPSSVMEGINIYAARKRIGQFLA
                     KKFPLTNADVVVPVPDSARPAALGYAQELGLPFEEGLLKDRYSKKGSMRSFIEPYQNN
                     RLEINKGIIPIPEVVEYKHVVIVDDSIVRGTSSAEIIRTLKMAGAKMVSLVVTYPPIR
                     YPCYAGIDFPSQDELIAYQHGTTKQSSNKIGSRVAQVIGADYVGYNDTTNLAKAIGMP
                     EEELCFTCSTGNYAPLGIRPVFKSRIEMKGES"
     gene            1616..1987
                     /locus_tag="VE308_03745"
     CDS             1616..1987
                     /locus_tag="VE308_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00119.1"
                     /translation="MRIAFIVIIAAGIIGVALVAMFASAIPTKDYDLSVDALKDEQSL
                     FTNARVVVKNTGRLPLSNVLVDYGSNKEPPIDILRPGDTLTLSPPEGVQLDQVTVTAE
                     PSIKIVQFYRTPWKLPGMIGS"
     gene            2022..2441
                     /locus_tag="VE308_03750"
     CDS             2022..2441
                     /locus_tag="VE308_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZB00120.1"
                     /translation="MEKDSHLDDEIKKFYTLKNIAVVGMSKNEEKAAHYVPKYLIDHG
                     YNVIPVNPTATEILGRKSYPKVSSVPDRIEIVDIFRRSEDVPSVVEDTLKKEGVKVIW
                     MQEGIYSKEAEKMAKEKDISAVYNRCMMAEHRRLFSG"
     gene            2475..2822
                     /locus_tag="VE308_03755"
     CDS             2475..2822
                     /locus_tag="VE308_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00121.1"
                     /translation="MSSIDRVSDGIRAVLEELEKQNIFFKVSVDTDASIVRIYGEDSH
                     YIKRASSGLGEVMELAYTTAEHHPYWAVLYNAAEICRTVLERWESDLTADQMSEMSWR
                     CEEIKMAIGRLEK"
BASE COUNT          902 a          617 c          734 g          741 t
ORIGIN      
        1 cagactgtct ttgaagcaat tgcagtcaaa tttttacttg cttgaaagga cactgcagtc
       61 ataaagaaga tatattttta atagcttaat gctcaatgta ctctatcttg gggtggaggc
      121 attggttcat gaaaactgcg gtgtgattgg gatcttttct cttgacgggc ataatgtcat
      181 accttttgtc atagactcgt taagagctct acagcaccgt gggcaggaag catggggcat
      241 tgcagttccc ggcaagccgc cattcagaaa gcttggactt gtctcttccg cagcctcaga
      301 cttcccaatg gtgatcaata aatacaagtc gcatacggcc atcggacacg tccgctattc
      361 cactcttggt aagagcacaa ttgaaaatgc acaacctctc aaagtaaaag atctctgtgt
      421 tgcacataac ggcacaattg ccaatgttga agagctgtca ggcatggtcg gcggttgcac
      481 ttttacccca caaacaatga gcgacactct tgtggcagct cagcgccttg tcatgcactt
      541 agctgacaag gaggatatgg cggaggcaat gcacatttta aaaggagaaa tggtcggctc
      601 tttctgcttt acatttctga cagacgaggg ttcggtgtat gcggctcgag atacacgcgg
      661 tttccgtccg ctagtccttg gatatcatag agaaaccaat acctacattg ttgcttcaga
      721 gtcatgtgca cttgcggcag taggagcgca actgacacgg gatgttgagc cgggcgagct
      781 cttgaagata agtaaggccg gtttggcttc gcaacatttt gcaaacgaaa gaccccatgc
      841 ccactgcgca tttgaatata catattttgc acacccgtcc agcgtaatgg aaggaatcaa
      901 catttatgcc gcaaggaaga ggatagggca gttccttgcg aagaaatttc ctttaacaaa
      961 tgcagacgtt gtagtcccag tccctgactc ggcacgacca gcggcgctgg ggtatgcaca
     1021 ggagctcggt cttccatttg aagaagggtt gctgaaggac aggtacagca agaaaggctc
     1081 gatgcgcagc tttatcgagc cgtatcaaaa caacagactc gagataaaca agggcataat
     1141 cccgatcccg gaagtggttg aatacaaaca tgttgttatc gtagacgaca gcatcgtaag
     1201 aggcacaagt tctgctgaga taatcagaac acttaagatg gcaggggcaa aaatggtgag
     1261 ccttgtggtt acatatcccc ctatacgtta tccatgctat gccggcatcg actttccatc
     1321 tcaggatgaa ttaatagcat accagcacgg cactaccaaa caatcaagca acaagattgg
     1381 ctcccgtgtc gcgcaggtaa taggggcgga ctatgtagga tacaatgaca ccaccaatct
     1441 tgcaaaggca atagggatgc cggaggaaga gctctgcttt acctgttcta cgggcaatta
     1501 tgcgcctcta gggatacgtc cagtgtttaa gtcaaggata gaaatgaaag gtgaatcata
     1561 ataatgataa taataataat aacagcttaa atattttcag actcaatctt ttctaatgcg
     1621 tatagcgttt attgtgataa tagcggctgg tataattgga gtcgctcttg ttgcaatgtt
     1681 tgcttctgca ataccgacaa aagattatga tcttagcgta gatgcactca aggacgagca
     1741 gtcactattt acaaatgctc gagtagttgt aaagaacaca ggcaggcttc ctctttcaaa
     1801 cgtgcttgtg gattatggaa gcaataaaga gcctccaata gacattctgc ggccaggcga
     1861 cacgctgacg ctttcgcccc ctgaaggcgt acagctcgat caggtcacag tgactgccga
     1921 gccaagcatt aagatagtac agttctatcg aactccatgg aaattacctg gcatgatcgg
     1981 atcctagtct atttaaataa tgtacaaacg aaattgatca tgtggaaaaa gactcacact
     2041 tggatgatga aataaagaaa ttctatacat tgaaaaacat cgcggtggta ggaatgtcaa
     2101 aaaatgagga gaaggcagcc cattatgtgc caaagtatct catcgatcac ggatacaatg
     2161 ttatccccgt caatcctacg gctacagaaa tcttggggcg caagtcatat cctaaggtca
     2221 gtagtgtgcc agacagaata gagatcgtcg atattttcag acgctcagaa gatgtgccct
     2281 ctgtggttga ggatactcta aagaaggagg gcgtcaaggt gatctggatg caggaaggta
     2341 tttacagcaa ggaagcagag aagatggcca aggaaaaaga tatttctgca gtctacaata
     2401 ggtgtatgat ggcagagcac aggaggctat tcagtggctg agttaaatta cacgcgttaa
     2461 gatactagta tctgatgtca agtatagaca gagtgtcaga tgggatcaga gctgttctcg
     2521 aagaacttga gaagcaaaat atctttttca aagtaagcgt cgacacagac gcaagcattg
     2581 tcagaatcta tggagaagat tctcactata tcaagcgggc atcttccggt ctaggagagg
     2641 tgatggagct agcatacaca actgcagagc atcacccata ctgggcggta ctatataacg
     2701 ccgcggaaat ctgcaggaca gttttggaga gatgggagtc tgaccttact gctgaccaga
     2761 tgagtgagat gtcatggcgc tgcgaagaga tcaagatggc tattggaagg ctggagaagt
     2821 agaggataat agtagcagga cgattttaaa aagaactaat aggatatcag caatacgatt
     2881 tccccaaaaa agggagaagc aatgaaaacc ttgataccat aactactatt attatcatct
     2941 tgccgatttt aaatccatgt ttatgcagcg agtagttata tggccatatg gggg
//
LOCUS       DASQTF010000159         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1065442,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000159 DASQTF010000000
VERSION     DASQTF010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065442"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..1424
                     /locus_tag="VE308_03760"
     CDS             465..1424
                     /locus_tag="VE308_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HZB00122.1"
                     /translation="MSDWDVVSVLTNLGINFESEGSKMRVQGIASIDKANESDLAFCW
                     YVGEKGLWYISRSNAGVILCKKEMQGLVHPNNMQLFVFTDNPRLTFVRVTREMTRQQQ
                     LVGISPRAVISEKSKIGSGCYIGAYAVIGDNCSVGNNTIIYDRVSLVQNCIIGSNCVI
                     QSGASLGSDGFAFERYETGELEKFPHKGNVRLGNNVEIYANCSIARGSLSDTWIDDGT
                     KLDALVHIAHNVTVGKNCELTAGTIIGGSTSVGDTSWTGLNSTLKNGIKVGRHVIVAS
                     GASVIHDIPDGDIVAGVPARSIKHKVNTDEVFLMAGQKKTATT"
     gene            complement(1427..2158)
                     /locus_tag="VE308_03765"
     CDS             complement(1427..2158)
                     /locus_tag="VE308_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZB00123.1"
                     /translation="MSNPLFSMNILAIGAHPDDIELGCGGLLLKASRQDHHNVFMYTL
                     TRGGASGDTEERTRESMQSAKFIGAKRLWIDNFEDTKLSLSSDLINHIEFFIKKADPD
                     IVLTHSLCDTHHDHRAIAASTMEAGRFVPNILAYEIPVTRDFKPQVYYDISDVIDGKM
                     ELINIFFSQRSKSFLQSNAIRGLAQYRALQSRFNNTVTCVEAFEVQKLFFGKEFKLMN
                     IPQEVNITKSTSQQMPKEVIETIST"
     gene            2358..2816
                     /locus_tag="VE308_03770"
     CDS             2358..2816
                     /locus_tag="VE308_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00124.1"
                     /translation="MIAVSVGRYAGAQSIPMVPPPFPENSPQEERIAPNDRAPPQIEI
                     LTTELHEGKNVFKVRITDESSLRTREVKYVHDGQLRVDGLFRDQNNVFKALIDIQAPS
                     RVVMVTAGDANGNIVSDFREYEIAKSQDIFTQVIDRLTQTLNYVQNMLGW"
     gene            2913..4283
                     /locus_tag="VE308_03775"
     CDS             2913..4283
                     /locus_tag="VE308_03775"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZB00125.1"
                     /translation="MAEKVWIAFFFVVIALWVLVLTASIYPRIYGIEILVDSDFDLTY
                     FTLPVLIFNLIYVFMLILIFALYLGGYRSVKKYQLEMKERVSERFRNQTELCSIVIPA
                     RNEESVIKRTVLDCLKQTYQKIEVIVICHNCSDRTYDEAKVNDPRVKVYDLQTKEAGK
                     GIALNFGVENSSGNYIMVLDSDGLLTRDFLENALPLFDGNYCAVQGRYIPSNRSFNFV
                     TRMLALEGDLWSTPFMTIRTCLGRRCPLGGTGYIVRKDILSKVGMFANHLVDDYELTF
                     RLLRNGYRIAFAPLSINYDEKPPTLDFMLRQRARWAKGFINLLGTRIADRSDIIGHIF
                     WLNPIAAATGFIMLMIPAFAALHYLIYDYYPYTYSYMPLSIWFMLTGLLFGLQAAVLV
                     KEYGWRGFAYAAQIPLFLPFSHYWFVSFIRAFTIKSWAGTKTAHGFMKEKGGENTWQM
                     QHDSIP"
     gene            4317..>4771
                     /locus_tag="VE308_03780"
     CDS             4317..>4771
                     /locus_tag="VE308_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HZB00126.1"
                     /translation="MQVEKNVVDIVTIAGTRPEIIKLADLVQLLDGQGNHALIYTGQH
                     YSDNMKSIFFDELKIKPDYDLRCDTSDVTTLKENMLKFLREIRPPHVLVYGDTNSSLA
                     GALAAKEVGSKLVHIEAGLRCFDLSVPEERVRIQIDSTSDYLFPPTELA"
BASE COUNT         1389 a          968 c         1058 g         1356 t
ORIGIN      
        1 agtccaaaag ttagctgaga gccctgtcga ggcaaaagtt atgccaattt ttgttagtgc
       61 aggctctagc aaatttctga gtttgttcag caatgaattt tcaacatgat tcttatataa
      121 taatttatat acaataaaag aaaaagctaa tcgagttttt cttttgtcat ctctgcgtta
      181 tattctgttt caagatttct gtctgggtct tttactttct tgcccattgc ttttgcacca
      241 gccttcactt tatctctgca ttctccgtgt cctgttctat gccgcttttc ctctcgactt
      301 tgtactcgac tttctcctct atatcagtca tagctattgc agcctactta tcaaatttat
      361 agacaaaaaa aaattgatta cgtaatttta ggataagatt gttaaagtag atcaacagta
      421 attctttaat aggtttttct tcgatacaga ctgtaatttc gcaaatgtca gattgggatg
      481 tagtgtctgt tttaaccaac cttgggatta actttgagtc agaaggtagc aagatgaggg
      541 tgcagggcat agcttctatt gataaggcaa atgaaagcga ccttgcattt tgctggtatg
      601 tcggcgaaaa agggctgtgg tatatttccc ggtcaaatgc aggggtgata ctgtgcaaga
      661 aggaaatgca aggtctggtt cacccgaata acatgcaatt gtttgttttt acagataacc
      721 ccaggcttac atttgtgagg gtgactagag aaatgacacg tcaacagcaa ttggtgggca
      781 tctcgccacg ggcagttatt tcagagaagt ccaagatagg atcaggctgc tatattggcg
      841 cctacgcagt aataggggac aactgttcag taggcaataa cacaatcatc tatgacagag
      901 tctcacttgt gcaaaactgt ataattggta gcaactgcgt gatccagtca ggagcgtcct
      961 taggatccga tggttttgct tttgagcggt acgaaactgg tgagttggaa aaattccctc
     1021 acaagggcaa tgtcagactt gggaacaacg ttgagatata tgctaactgc tctattgcgc
     1081 ggggatcgct ctccgacact tggatagatg atggaacaaa acttgatgct ctcgtccata
     1141 ttgctcataa tgtaactgtt ggaaagaatt gtgaactcac tgctggaacg attattggag
     1201 gcagtacaag cgtaggagac accagctgga cgggtctgaa ttcaacattg aaaaatggga
     1261 tcaaagtagg cagacacgtc attgtggcct caggtgcttc ggttatacac gacatccctg
     1321 atggcgatat tgttgctggc gttccagcta ggtcaatcaa acacaaggta aacaccgacg
     1381 aggtgttcct tatggcaggc caaaagaaaa cggctacaac ctaaacctag gtgcttattg
     1441 tctcaatgac ttcttttggc atttgttgag atgttgattt agttatattt acctcctgtg
     1501 gaatgttcat cagcttgaac tctttgccaa aaaagagctt ttgcacttca aaagcttcca
     1561 cgcacgttac cgtattattg aatctgcttt gcaatgcacg atactgtgcg aggccgcgaa
     1621 tggcatttga ctggaggaac gacttgcttc tctgcgagaa gaaaatattg ataagctcca
     1681 tcttaccatc gataacatcg gaaatatcat agtacacctg aggcttgaag tcgcgagtta
     1741 cgggaatctc ataagctagt atgtttggaa cgaatcttcc agcctccatc gtagaagcag
     1801 ctattgcgcg atgatcgtgg tgtgtatcgc agagcgagtg cgtcagcaca atgtcaggat
     1861 cagccttctt tatgaaaaac tcaatatgat tgataagatc acttgaaagt gataacttgg
     1921 tatcttcaaa attgtcaatc cataatcttt ttgctcctat aaattttgca gactgcattg
     1981 attcccttgt gcgctcctcc gtatcgcctg atgctcctcc tcgcgtcagg gtatacatga
     2041 agacattatg atggtcctgt cgcgaagcct ttagcaacag gccgccgcat ccaagttcaa
     2101 tgtcatcagg gtgcgccccg atggcaagaa tattcatcga aaagagtggg ttcgacactt
     2161 atttatataa ttggcaattc ccctctgtca taatataata gatgatttat ctgccattgc
     2221 ttgaaccgct ataatccatg gaacgacaga ttatactata taacgctcta tattatataa
     2281 gagaagccgt gacttttata ttattttata ccaattctct tttattgaag gaattcgctc
     2341 ttgctttttt gctattgatg atagcagtgt cagttggaag atatgctggc gcacaatcaa
     2401 tcccaatggt gcctcctcca tttccagaaa acagcccaca agaagaaaga atagctccaa
     2461 acgacagggc tcctccccag atagaaatac ttactaccga gttgcacgag ggcaagaatg
     2521 tctttaaggt taggataaca gatgagtcaa gcctgagaac aagagaggtc aaatacgtgc
     2581 atgatggtca gctgagagtt gatggactct tccgagacca gaacaatgta tttaaggcat
     2641 taatagacat acaggcaccc tcacgggtgg tcatggttac tgcaggcgat gcaaatggga
     2701 atatcgtctc agactttaga gagtacgaaa tcgccaagtc ccaagacata ttcacacagg
     2761 taatagatag actgacacag acactgaatt acgttcagaa catgttaggc tggtaaaatc
     2821 aagcatagac catagtaata aataaatgcg aatatagcac gatggatttt agtaacgctg
     2881 tgatcttaat tcattatgga aggtgtaact gattggctga aaaagtatgg attgcattct
     2941 tttttgttgt aattgcactt tgggtcctag tgcttacagc gtcaatatac ccccggatat
     3001 atggaataga aatcctagta gacagcgatt tcgacctaac ttattttacg ctgccagtac
     3061 taatcttcaa cctaatatat gtattcatgc taatcctgat attcgcatta taccttggcg
     3121 gctaccgcag cgtcaagaaa taccagctag agatgaagga aagggtatct gaaaggttcc
     3181 gaaaccagac agagctatgt agtatagtaa tccctgcaag aaacgaggag agtgtcataa
     3241 aaagaactgt acttgactgt cttaagcaga cctatcaaaa aatcgaagtc attgtaatat
     3301 gccataattg ctctgacaga acctatgacg aagccaaggt taacgatcca agggtaaaag
     3361 tatatgatct acagactaag gaggcgggca aagggatcgc attgaatttt ggagtcgaga
     3421 attcaagtgg caattatata atggtgctcg acagtgacgg actgctgaca cgggatttcc
     3481 ttgagaacgc cctacctcta tttgacggaa attactgtgc agttcaaggc aggtacattc
     3541 caagcaacag gagctttaac tttgtaacca ggatgcttgc actcgaaggt gatctttggt
     3601 ctactccatt tatgacgata aggacctgtc ttggtcgccg gtgtccgctt ggaggcacag
     3661 gctatattgt cagaaaagac atcctatcaa aggtaggcat gtttgccaat caccttgtag
     3721 atgactacga gttaaccttc cggttgctaa ggaatggcta caggatagca tttgcgccgc
     3781 tgtccataaa ctacgatgaa aaaccaccga cacttgattt catgctgagg cagagagcaa
     3841 gatgggctaa aggattcatc aatttgcttg gaactagaat tgcggatcgg tcagatatta
     3901 ttggccatat tttctggcta aatccaattg ctgcggcaac tggctttata atgctcatga
     3961 tccctgcttt tgcagctctt cactacctga tatatgatta ctacccttat acctattcat
     4021 atatgcccct gagtatatgg tttatgctga caggcttgct ctttggattg caagctgcag
     4081 ttctagtaaa agaatatggc tggcgtggtt ttgcctatgc ggctcagata cccctgtttc
     4141 tgccattttc acattactgg tttgtcagtt tcattagggc atttacaatc aagtcatggg
     4201 ccggtaccaa gaccgcacat ggatttatga aggaaaaagg gggagagaac acgtggcaga
     4261 tgcagcacga ttctataccc tagaaataca atgaagacgc gtaataatcc gttaagttgc
     4321 aagtagaaaa aaatgttgta gacattgtta caatagctgg cacaagaccg gagatcataa
     4381 aactagccga tctggtacaa ttgcttgatg gccaaggcaa ccatgcccta atctacacag
     4441 gacagcatta ttctgacaac atgaagagca tcttttttga tgagttaaag atcaaaccgg
     4501 attatgacct gcgctgcgac acatcggatg ttactacgct gaaagaaaac atgctaaagt
     4561 tccttcggga gattaggcct ccacatgttt tagtttatgg cgacaccaat tcgagcctag
     4621 caggtgcatt agcagcaaag gaagttggct ccaagcttgt tcacattgaa gcgggcctcc
     4681 ggtgctttga ccttagtgtt ccggaagaaa gggtaaggat acagatcgat tctacatcgg
     4741 actacttatt tcctccaacc gaacttgcta g
//
LOCUS       DASQTF010000160         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_966831,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000160 DASQTF010000000
VERSION     DASQTF010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966831"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VE308_03785"
     CDS             <1..706
                     /locus_tag="VE308_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZB00127.1"
                     /translation="ANFLYMLRGIEPSPEEAKVMDICLILHAEHSFNASTFAAREIAS
                     TRAHMYASIGGAIGALSGELHGGANVQVMKMLLEIGDPASVDRWVANRLQSGGRIMGM
                     GHAVYRTTDPRADILARLSARICREKNNKWYEITKRIEETTKKWMLENKHQGIYPNVD
                     LYSASVYYSMGIPIDLNTPIFATSRIAGWSAHVIEEKFAEAAPKPALYRPKAVYVGKY
                     CGPMGCEYTPISSRSK"
     gene            complement(971..1195)
                     /locus_tag="VE308_03790"
     CDS             complement(971..1195)
                     /locus_tag="VE308_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00128.1"
                     /translation="MLGSKDRVDDQFVEMIDDLVVMSENDSELAEGLKWIDFQSQKNG
                     LTFYEMAMIILRKHMVERRAKEWLREKINQ"
     gene            1409..2491
                     /locus_tag="VE308_03795"
     CDS             1409..2491
                     /locus_tag="VE308_03795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZB00129.1"
                     /translation="MKIKFGFTQGLNVARLGLDEQQIMTGAQMADRLDYDSIWAMDHS
                     NVPQWKNAVVNDAWLLLAALGAVTRNVELGTCVTDAIRRHPSAIALSTITLDRITQGR
                     GILGIGAGEAQNVVDFGIEFSKPVTKFKEQLEVIELLFDSDPEHRVNYNGEYYKLINA
                     CLQAKSIRNPRPPVYIAAGAPKTLQLCAEYGDGWIPIGYTPELFAYHANVIRDHAKKR
                     DRDLTNFQFANDVDIYFTDDGEEAWNKMKNAVKVSLYKPELLKVHDLPQDSDFDFRRY
                     FTEYAMNKPELMEQMKRAALKIPDSVARSAIGVGKPDDVITMLERYIKAGTNHFIIRF
                     WGEGYFKNIEMFGSKVIPYFRDEHRK"
     gene            complement(2664..>3641)
                     /locus_tag="VE308_03800"
     CDS             complement(2664..>3641)
                     /locus_tag="VE308_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00130.1"
                     /translation="HKDTTIALDPLRPTESHFTFVSHAHVDHLHKRGKEKKNKSQVLA
                     SKETALIAQARGYKIGNPAEQYDGFRLVDTGHILGSRGLLIGEEIYYTGDISMRERAF
                     MKPAKVPHAKTLIIESTFGRPQYVFPPISEITHKTNKIISEMYDLGIPVILMGYTLGK
                     AQLLTKLFSHWDPIIYDSVAKINSVYSELGVKLESVMTHLQAVEQGLMSKNRPWVMIA
                     PLMSSKYGLLKEMKNTYGAVTIGFTGWAIGSRYRYMMGLDYVMPLSDHCDYNELVAAV
                     KQCKPEKVYTFHGFAKEFAQSLSRIGFDAEAVGHGKSGKEKTVSLDSFL"
BASE COUNT          997 a          838 c          820 g          986 t
ORIGIN      
        1 tgctaacttt ctatacatgc ttagaggaat cgaaccctct cctgaagagg caaaggtaat
       61 ggacatttgt ctcattttac atgcagagca cagctttaac gcctctacat ttgctgcacg
      121 cgagatagca tcaactaggg cgcatatgta tgccagtatc ggtggtgcta taggcgcact
      181 ttctggcgag ttgcatggtg gcgcaaacgt ccaagtgatg aaaatgctgc ttgagatagg
      241 cgatcctgct agcgtcgaca ggtgggttgc aaaccgcctc cagtcggggg gcaggatcat
      301 gggcatgggt cacgccgtct acaggacgac tgaccctcgg gctgacattc ttgcaaggtt
      361 gtcggcgcga atctgccggg aaaagaacaa caagtggtat gagatcacta agcgcattga
      421 ggaaactaca aagaagtgga tgcttgagaa taagcaccag ggaatatatc caaatgttga
      481 tctttacagt gcctcagtat actatagcat gggaatcccc attgatctta acacgcctat
      541 ctttgcaaca tcaaggatag caggttggtc agcccacgtt attgaggaaa agtttgccga
      601 agccgcaccc aagcctgcgc tctatcgccc aaaggcagtt tacgtaggca agtactgtgg
      661 cccgatggga tgcgagtata cacccatttc aagcaggagc aagtaagagg agcagtaaat
      721 atattctgcc gcccccgacg gctgaccacc aacaacagcc agaccaagga tgtcggatca
      781 cgcggaaaca actggcgaag aagatgttaa attatcaatg ttcctacaca gagactcctt
      841 agccggatac cttttcctct tcgatcctaa tagaggtatc ttttgtagcc accagagatc
      901 acagtataat taggatgacg acattagtgc ttttgatggc tgcagggctt tctgctacta
      961 cctattatta ctattgattg atcttctccc tcagccactc tttggccctc ctctcaacca
     1021 tgtgctttct cagtattatc attgccatct cataaaatgt caaaccgttc ttttgcgatt
     1081 ggaagtcgat ccacttcagc ccttctgcca gctcagagtc gttctcagac atcacaacaa
     1141 gatcgtctat catctccacg aactggtcat caaccctatc tttgctccct agcatttctg
     1201 ctgtgtgctt tagtacagta gcgattttac atagtgattc aagcagtatt gacactttga
     1261 atatattata ttgacaaagc tgtggcaaat tctgttgaaa acttggcgtt tcagcattca
     1321 aagacttaaa atacatcctt caattgattt taatctaaaa tgaatagaag gtactacctt
     1381 ctcctagtgg ctgaggagga tttgtaaatt gaagataaaa tttgggttta ctcaggggct
     1441 caatgttgct cgcctcggac tagatgaaca gcagataatg actggtgcgc agatggcaga
     1501 cagactagac tatgactcta tttgggccat ggaccactca aatgtgcctc agtggaagaa
     1561 tgcagtagtc aacgatgcgt ggctcttgct tgctgcactt ggcgcagtta cccgaaatgt
     1621 tgagcttggg acatgtgtta cagatgcaat ccgcaggcac ccttccgcca tcgcgctttc
     1681 aactattaca ctcgacagga taacacaagg aaggggcatt cttggtatcg gagccggtga
     1741 ggcccaaaac gtcgtggatt ttgggatcga gttcagcaag cctgtcacaa aattcaaaga
     1801 gcagctcgag gtaatcgaac tcctatttga ctctgatcca gagcaccgcg tgaactacaa
     1861 tggtgaatac tacaaactga taaacgcatg tcttcaggca aagagcataa gaaatccaag
     1921 gccaccagtt tacatcgcag ctggagctcc aaagacactg caattatgtg ccgaatatgg
     1981 cgacggctgg atccccattg gctacacacc agagctcttc gcctatcatg ctaacgttat
     2041 cagagatcat gcaaagaagc gtgaccgcga cctaaccaat ttccagttcg caaatgatgt
     2101 tgatatttac ttcacagacg atggcgagga agcgtggaac aagatgaaaa atgcagtcaa
     2161 agtgagccta tataaaccag agctcctgaa ggtgcatgac ctaccgcagg actctgactt
     2221 tgactttcgc agatacttca ctgaatatgc aatgaacaaa ccagagctaa tggagcagat
     2281 gaagcgcgca gccctgaaga tacctgacag tgtagcccgt agtgcaattg gtgtcggcaa
     2341 gcctgacgac gtgatcacga tgctagagcg ctatatcaaa gctggaacaa accactttat
     2401 cattagattc tggggagaag gttactttaa gaatattgag atgtttggat ccaaggttat
     2461 tccctatttt agggacgagc acaggaagtg atatgatggt tgttttcttt taacgttgca
     2521 agtagagtta ttaaccctct tgcttgtttc ttttatgtgc cagtgtgcgt ccatatgtat
     2581 ataattagaa aagcttttta aaatatttct aacgaaattg tatgcttatg ctgttgcttg
     2641 ctcgcaaaga ataattttac gctctacaga aatgagtcaa gcgacactgt tttctctttt
     2701 ccactcttgc catgtcctac agcctcggcg tcaaacccta ttcgcgataa cgattgagca
     2761 aattctttgg caaacccatg aaaagtatag accttttcag gcttgcactg cttaactgcc
     2821 gctaccagtt cattatagtc acagtgatca gacagcggca taacataatc aaggcccatc
     2881 atgtatctgt agcgactacc tattgcccag ccggtgaatc caatggtcac ggcgccgtat
     2941 gtgttcttca tctcctttaa taggccatac ttggaagaca tgagaggggc gatcataacc
     3001 caaggtcggt tctttgacat aaggccctgc tccactgcct gtaagtgagt cataacgctt
     3061 tcaagtttta ctccaagttc agagtaaact gagtttatct tggcaacaga gtcgtatatt
     3121 atagggtccc aatggctgaa tagctttgtt aaaagttgag ccttaccaag tgtatatccc
     3181 atcaatatta caggtatgcc aaggtcgtac atttcagaaa ttattttatt tgtcttgtgt
     3241 gttatttcag aaatcggcgg aaagacatac tgaggcctgc caaaggtcga ctcaattatt
     3301 aacgtcttgg catgcggcac ttttgctggc ttcatgaacg cgcgctctcg cattgatata
     3361 tccccggtat agtagatctc ctcgcctatc aacagaccgc gcgatcccag gatatgccct
     3421 gtatctacaa gtctaaagcc atcatactgt tcggcagggt taccaatttt atagcccctc
     3481 gcctgtgcaa tcagagcggt ctcttttgat gctagcacct gacttttgtt ctttttttct
     3541 ttaccacgct tgtgaaggtg gtcgacgtga gcatgagata caaacgtaaa atggctctca
     3601 gtaggacgca aagggtccag cgcgattgta gtatctttgt g
//
LOCUS       DASQTF010000161         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_420307,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000161 DASQTF010000000
VERSION     DASQTF010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420307"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VE308_03805"
     CDS             complement(<1..662)
                     /locus_tag="VE308_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZB00131.1"
                     /translation="MNADNLSALIVGLGVVALIVGFGVYFNSPELNNAASGQQKSFVP
                     AAIIEDNGTITRLQIDKSQFKLAPELTKIAGFVNSEPITLADLRGKVVLVDFWTYSCI
                     NCIRTIPYLNTWYEKYADNGLVIVGVHTPEFEFEKDYNNVRAAAEKSDIRYPIAQDNE
                     KETWKAYENRYWPRKYLIDPEGYVRYDHIGEGAYAETEKVIQSLLAERTEYIGANVTI
                     DQS"
     gene            complement(659..1423)
                     /locus_tag="VE308_03810"
     CDS             complement(659..1423)
                     /locus_tag="VE308_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZB00132.1"
                     /translation="MAETSVLISALAGAGSFFSPCILPIIPAFVSYLSGTALTEIQSQ
                     NPSLNGASVAMKKSTRLNIFLNTVYFVLGFSLVFAIMGVVLNSFLANLGTGFQNTFQV
                     IGGAVIIAFGGYLILSMKLRILNFEKRMRNLPRFKTSYLTSFVFGAAFASGWTPCVGP
                     ILGTTLTLAATSPGAAYNSLLAYSLGLGVPFLITGAFFSQSTKIIRKMIKHLKYFNPL
                     MGAILIVLGILVLTNQLSVIGNFPLASEIINLEGGL"
     gene            complement(1500..1772)
                     /locus_tag="VE308_03815"
     CDS             complement(1500..1772)
                     /locus_tag="VE308_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00133.1"
                     /translation="MAVLVALLVVYAKIYFKTRATFTVGLAVFTSMLMLHNGIAVYGY
                     FAMAPLYSDELLPYFAGIHIAELIGLIALLKVTVWPEGLKTAKAAP"
     gene            complement(1848..2162)
                     /locus_tag="VE308_03820"
     CDS             complement(1848..2162)
                     /locus_tag="VE308_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZB00134.1"
                     /translation="MPDPDAKRLLWFLFAGSRGGENRIRIIDFLKKRPYNINQLADTL
                     GVDYKLVQHHIGVLERNNMVTKTGEKYAVLYFISNYLEANIEAFNEIQAAIEKQNQSG
                     KK"
BASE COUNT          618 a          506 c          488 g          638 t
ORIGIN      
        1 gattgatcaa ttgtgacgtt tgcaccgatg tattctgtgc gctccgcaag cagcgattgg
       61 atgacttttt cggtttcagc gtatgcgccc tctccgatgt gatcgtaacg aacgtaacct
      121 tcagggtcga tcagatattt ccgcggccag tagcggtttt cgtatgcctt ccacgtttcc
      181 ttttcgttat cctgtgctat aggatatcgg atgtcggatt tttcagctgc agccctgacg
      241 ttgttgtagt ctttttcaaa ctcaaattct ggtgtgtgta cgcctacaat caccagaccg
      301 ttatctgcat atttttcata ccatgtgttc aagtatggaa tagttctgat gcagttgatg
      361 cagctgtacg tccagaaatc taccagtacc acctttcccc taagatctgc aagagttatt
      421 ggctcggagt taacaaagcc ggcgatcttt gtcagttctg gagccagctt gaactgtgac
      481 ttgtctattt gcaatcttgt tattgtgccg ttatcttcaa ttatagcggc aggcacaaag
      541 ctcttctgct ggccagaggc agcattattg agctctgggc tgttaaagta tactccaaag
      601 ccgacgataa gtgctaccac cccaaggcct actatcagtg cactcaggtt gtctgcgttc
      661 atagtcctcc ttccaagtta attatctcac ttgcgagagg aaaattgccg attacgctca
      721 gttgatttgt tagtacaagg atacctagta cgatcagtat cgcacccatc agcgggttaa
      781 aatatttcag atgcttgatc atcttgcgaa ttatctttgt tgactgcgaa aagaaggcac
      841 ctgttatgag gaatggtact ccaagtccaa gtgaatatgc aagaagggaa ttgtaggcgg
      901 cgccaggaga tgtagcggca agcgtaagcg ttgtcccaag tattgggccc acacagggtg
      961 tccaaccaga agcaaaggca gctccaaaca caaaagatgt cagatagcta gtcttaaaac
     1021 gaggcagatt tctcattctc ttttcaaagt tgagtatgcg tagcttcata gacaagataa
     1081 ggtatcctcc aaatgcaatg attactgcgc cccctatcac ctggaaagta ttctgaaagc
     1141 cagtgccaag gttggcaagg aagctattca ataccacacc catgatggca aacacaagtg
     1201 agaatccaag aacaaaatag acagtattca aaaagatgtt gagtctggta gattttttca
     1261 tggctactga agcgccgtta agactcgggt tttgactctg gatctcggta agggctgtgc
     1321 cagagaggta tgaaacaaat gctgggatta tcggcaagat gcatggagag aaaaatgatc
     1381 ctgctcctgc cagagcagat attagaacgc tagtctctgc caattcatac agcatctgcc
     1441 aatttttgga ttaatacttt tttccagatc tctaccagat ctttagccgc atctgcagtc
     1501 tatggggcag ctttagcagt ctttaatccc tcaggccaga ctgttacttt cagaagagct
     1561 attaacccta tgagctccgc aatgtgtatc ccagcaaagt agggcaacaa ctcatcagaa
     1621 taaagaggtg ccattgcaaa gtacccgtaa actgctattc cattgtgaag catcagcatg
     1681 ctagtaaaaa ccgcaaggcc gactgtaaaa gtggctctgg tcttgaaata tatcttagca
     1741 tacaccacca acaatgcaac caatacagcc atgtttgcca tgcttactag ggagctaatg
     1801 tccatcagca gagccattat catcagacgt cagagaaatt gacttattta cttttttcca
     1861 gattggttct gcttttctat tgcggcctgg atctcattga atgcctcaat gttggcctca
     1921 aggtaatttg aaataaagta aagtactgca tacttttcgc cagtctttgt gaccatgttg
     1981 ttcctttcaa gcactccaat atgatgctgc accagcttgt agtcgacccc aagggtatca
     2041 gcaagctggt taatgttgta aggtctcttt ttgagaaaat caattatcct tatcctattc
     2101 tcgccgccac gggagccagc aaaaaggaac caaagcaggc gtttagcatc gggatcaggc
     2161 atgtattacc acgctcatta tgagagcttg aaatttaaac tgaatatgat accgtatacc
     2221 ttatatcatt gtcttgccta aacaaagcaa
//
LOCUS       DASQTF010000162         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1069153,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000162 DASQTF010000000
VERSION     DASQTF010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069153"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..116
                     /locus_tag="VE308_03825"
     CDS             3..116
                     /locus_tag="VE308_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZB00135.1"
                     /translation="MPGETFEFTFTKVGEYAYHCEPHPHMQGSVEIVESFA"
     gene            complement(113..481)
                     /locus_tag="VE308_03830"
     CDS             complement(113..481)
                     /locus_tag="VE308_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00136.1"
                     /translation="MSAQKDVSISLAESSIVAGNDLHGTVVINYRGRFDSVVVNSQIE
                     NSSDVFSYASLNEKNIKHPYARLSIFKSELAGRNTINFTATTNHVPNGEYSNVKFRVS
                     IIQEHKEVVSDIAYTKILKA"
     gene            complement(601..1389)
                     /locus_tag="VE308_03835"
     CDS             complement(601..1389)
                     /locus_tag="VE308_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00137.1"
                     /translation="MIRDLLFTKRLMGRKMALNRRAMIVALSLAAIPLTAILIAVATD
                     RGKRKSLQTSRLLRQTRAKAGIVTTTATTAVHKQEQKEKPQVQPQPSSQGSEKKAATT
                     TAMVTTNTTIADKDITAPPEVLPSIEPSGTSDSSVISSSSQTSKEEEISVSDEARKAR
                     ESLKELIVTAIKEAKDSAKGTGKRIKEQTINIAATADSKNIHSLGDNVNALVGLFEET
                     MTEIRKERYDEQIKLLDGYKDLLRTHIKVVDARARMASKLKPGA"
     gene            1532..1840
                     /locus_tag="VE308_03840"
     CDS             1532..1840
                     /locus_tag="VE308_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00138.1"
                     /translation="MNADHFEIDRLIEDLAKLYSTACATSWFKIGDNKHPSREEFRHK
                     VVEFMKHFEYTLATFPQTPAADQFRKQARMSLQKEIAKVLAGENKDVEKRYKYYVDYS
                     "
     gene            complement(1837..2451)
                     /locus_tag="VE308_03845"
     CDS             complement(1837..2451)
                     /locus_tag="VE308_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZB00139.1"
                     /translation="MIKRKVKRMLKGRDPAHDFHHIMRVYNNAKLIGRREGTNMEILL
                     PAVLLHDIVVYPKGCAKSAKSSDESADLAENILQGYDYPQDQINQICYCIRAHSYSKR
                     LMPASLEGRILQDADRLDALGAIGIARTFSIGGSENRTFYNADDPFCRSDRDLDDMQW
                     TVDHFQTKLLKLEDAMHTETAKKIAQERTRFMMLFIRQLQKEII"
BASE COUNT          652 a          537 c          536 g          794 t
ORIGIN      
        1 tgatgcccgg tgaaacattc gaatttacat tcactaaggt tggtgaatac gcatatcact
       61 gcgagccgca tccgcacatg caaggtagcg tcgaaattgt agagagcttc gcttaagcct
      121 ttaatatttt tgtataggcg atatcgctta ccacttcttt atgctcttgt attatcgaca
      181 cgcggaattt aacattagag tattcaccat tgggaacatg gttagtagtg gccgtaaaat
      241 tgatggtatt tctgccggca agctctgact taaagattga tagtcgtgca taagggtgtt
      301 tgatgttttt ctcattgaga ctagcgtatg agaaaacatc acttgagttc tctatctggg
      361 agtttactac cacgctgtca aatctccccc ggtagttgat gactacggtg ccatggaggt
      421 cattccctgc tactatggag ctttcagcca aagaaattga gacgtccttc tgtgcgctca
      481 agcagataac aagatctcat gacggctgat ttaaaccaac ttgaggcgcg cttgtatttt
      541 tcactgaaaa aaaggttaga attacaactt cgcttgcaca ctagatagtt agaatgtgca
      601 tcaagcgcca ggttttagtt tgcttgccat tctggctctt gcatcgacta ccttaatgtg
      661 tgtccgtaaa agatccttat agccgtccag caactttatc tgctcatcgt agcgttcttt
      721 acggatctca gtcattgtct cttcaaagag gccaaccaag gcattgacat tgtctcctaa
      781 ggagtgaata ttttttgaat cagctgtagc agcaatgttg attgtttgtt cttttattcg
      841 ttttcctgtt ccctttgcag aatcttttgc ctctttgata gctgtgacga tcagttcttt
      901 cagcgattct cttgcctttc tagcttcgtc agaaactgat atttcttcct cttttgaagt
      961 ttgagaagaa gagctgatta ctgagctatc agaagttcct gaaggctcta ttgacggtag
     1021 tacttctgga ggggcagtaa tatctttatc agcaatagtt gtgttggtag taaccatggc
     1081 agtagtcgtc gctgcttttt tctcagaccc ttgcgaagaa ggttgcggtt gcacttgtgg
     1141 tttttccttt tgttcttgtt tgtggacagc agtagtagca gtagtagtaa caatacccgc
     1201 ttttgctctt gtctgtctta agagtcgact tgtttgtagt gatttacgct ttcctctatc
     1261 agtagcgaca gcaattaata tcgcagtcaa aggaatggca gctagagata atgctactat
     1321 cattgccctt ctgtttaacg ccatttttct tcccattagt ctctttgtaa acaataggtc
     1381 tctaatcatt catttaagaa ttcagaatat aatgactaga aataattatt acatgcctat
     1441 caaaagtatt ggattcttgt aggtgtggac gcatcataca cacacataga aatacacaca
     1501 caattaatgc aagtatgagc taacctcaat catgaacgcc gaccattttg agatagatag
     1561 gctgatagaa gatcttgcca agctctactc cactgcttgc gcaacatctt ggttcaagat
     1621 aggggataac aagcatccat ctcgagaaga attccgacat aaggttgtgg aattcatgaa
     1681 gcactttgaa tacacacttg ccacttttcc acagacaccg gcagctgatc aattccggaa
     1741 acaagcaagg atgagcctac aaaaagaaat tgcaaaggtg cttgcaggtg agaataagga
     1801 cgtagaaaag cgctacaagt attatgtaga ctacagctag atgatttcct tttgcaactg
     1861 tctgatgaaa agcatcataa atcttgtacg ctcttgagca attttcttcg cagtctcagt
     1921 gtgcatagcg tcctccaatt ttagcaattt ggtctgaaaa tggtcaactg tccattgcat
     1981 atcgtctaag tccctgtctg acctgcaaaa cgggtcgtca gcgttgtaaa aggttctatt
     2041 ctctgaacca ccaatactaa atgtgcgcgc aatccctatt gcgccgagcg cgtcaagcct
     2101 gtcagcatcc tgaagtattc ttccttcaag gcttgctggc ataagtctct tggagtagct
     2161 gtgcgcccgt atgcagtaac atatctgatt tatctggtct tgggggtagt cgtagccctg
     2221 caggatattt tctgccaagt cagcgctttc gtctgaagac ttggctgact tggcgcatcc
     2281 cttaggatag acaacaatgt catgcaacaa gactgctggg agtaatatct ccatattggt
     2341 gccttcgcgc ctgcctatca gcttggcatt attgtagaca cgcatgatgt ggtggaaatc
     2401 gtgagcaggg tcccggccct ttagcatcct ctttactttt cttttgatca tctcctgcat
     2461 aacagcgatc gattcctact acttgttcat taataaaagt ggctagtttg gttaggtat
//
LOCUS       DASQTF010000163         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1069422,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000163 DASQTF010000000
VERSION     DASQTF010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069422"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VE308_03850"
     CDS             complement(<1..741)
                     /locus_tag="VE308_03850"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZB00140.1"
                     /translation="MPPAISTMHFPFELKKDQLEAAKAWIDNGCRGSVIFSTGTGKTE
                     IAFECARWAAKLSGQDCFTILFLVPRIVLIEQNVRRLRRYGISDDHIGIYYGERKDIR
                     EITISTYQSAINNYNLIRSANMVILDEVHLVSESAVAFDSIFDIIVEDSHKAILGLTA
                     TINEKDPKYQTILTVVPPIKKYMIKDAVTDGRLAKPIVKPVEVSFTSEEQKSYDEAST
                     AIKDISRKLQAYDPMKMTRILMRGGSRAS"
     gene            802..1335
                     /locus_tag="VE308_03855"
     CDS             802..1335
                     /locus_tag="VE308_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HZB00141.1"
                     /translation="MKPLIYMLHQDDPFKCTAAKLVRFRLAAPVKFISRTTIVLNPFS
                     EVPVLRHDHTVADSVCAIDCSWERADEVLKHRRLAARGIARRLPEMLAANPTNYAKLG
                     KLSSVEALAGAFYILNEKELAIKMMDKFKWGHTFLQLNANLLDDYADAETEEQVVKLE
                     EEYFPQLVRNYFRKTNE"
     gene            1375..1654
                     /gene="rnpB"
                     /locus_tag="VE308_03860"
     ncRNA           1375..1654
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VE308_03860"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            complement(1852..2142)
                     /locus_tag="VE308_03865"
     CDS             complement(1852..2142)
                     /locus_tag="VE308_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZB00142.1"
                     /translation="MINRDRIDIINQILKAANGGANKTRVMYKVNLSYAQLKKILTAL
                     TEKDLIRYDKNTRILWTTEKGLMFLDIYNRMSDMIEEGQQQHQMWIHREEEV"
BASE COUNT          690 a          521 c          515 g          728 t
ORIGIN      
        1 tgatgcccgc gaccctccac gcattagtat ccttgtcatc ttcatcggat cataggcctg
       61 aagctttctt gagatatcct ttattgctgt acttgcctca tcataacttt tttgctcctc
      121 cgatgtaaag ctgacttcta ctggcttgac gattggcttt gctagtctgc catccgtgac
      181 tgcatctttg atcatgtatt tctttatagg cgggacgaca gttaggattg tttgatattt
      241 tgggtccttt tcatttatgg ttgcagtgag tcctagaatt gccttgtgcg aatcttctac
      301 aataatgtcg aagatactgt caaatgcaac tgcgctttcg ctgacaagat gtacttcatc
      361 aagtattacc atgttggcac tccttatcag gttgtaatta ttgatggcac tttggtacgt
      421 gctgatagtg atctctctaa tgtctttgcg ctcaccgtag tatatgccga tgtggtcatc
      481 agaaatgcca taacgccgca gacgcctaac gttctgctct attagcacta ttcttggaac
      541 tagaaataat attgtaaagc aatcctgtcc tgacagctta gctgcccacc tcgcacactc
      601 aaatgcgatc tcagtcttgc ccgtgccggt gctaaatatg acagatccac ggcagccgtt
      661 gtcaatccat gctttggcag cctccaattg atcctttttt agctcaaatg gaaaatgcat
      721 tgtagatatc gctggaggca cacaacaaat gaaagtcaag cgaataaaaa ctttaatgat
      781 cttggctaaa catgatatag tatgaaaccg ctaatctaca tgctccacca agatgatccg
      841 ttcaagtgta ccgcagccaa gctggtcagg ttccgcttag cagcgccagt caagtttatt
      901 tcgaggacta ctattgtgct caaccctttc tcagaagtac ctgttttgag acacgaccat
      961 acggtagcag attctgtatg cgctattgac tgctcatggg agcgcgcaga cgaggtgcta
     1021 aagcatcggc gccttgccgc tcggggaata gcgagaagac tccccgagat gctggctgcg
     1081 aacccaacca actatgccaa gctgggcaag ctatcgagcg ttgaagccct tgcaggtgca
     1141 ttctacatct tgaatgaaaa ggagcttgcg ataaaaatga tggacaagtt caagtggggc
     1201 catacgtttc ttcagctcaa tgccaacctt ctggacgact atgcagatgc agaaacagaa
     1261 gagcaagtgg ttaagctgga agaggagtac tttcctcaac tggtaagaaa ttactttagg
     1321 aagactaatg aataatgcta ggttttaata tcaattctgt ccatgatact gcgtgtgagc
     1381 cagtgggtgg gtcactcgaa agagaggaaa ctcctccctc actgcaggca cccgaattag
     1441 cgataattag tcggagacga ctggcaacgg aacagaaaag atatccagtc agaatagtaa
     1501 gatgatggtg agtagcctga tatatctgat actggaatga aactgccgac ccaggtggag
     1561 caaggctaaa tagagcataa ggtcctgcac ctgctcaggt tggctgctca gcagaatccc
     1621 gcttagaaca gaaggagagt tattgctggc acgcaccata atttatcaaa acttgcatca
     1681 atcactacat tagaatatcg ataaaaaaat tgtgtcttat tgtagcataa tatggaataa
     1741 cagcaggcct atctgttaca tagtatcatg attagagggt aatagattcg agttctttaa
     1801 tcagatggaa gaaaccaata atgattggcg acttatacgc tctgctctct ctcaaacttc
     1861 ttcttcccta tgtatccaca tttgatgttg ttgttgtcct tcctctatca tatcgctcat
     1921 ccgattgtat atatcaagaa acattagacc tttttcagta gtccacaata ttcgagtatt
     1981 tttgtcgtag cgtattaggt ccttttcagt tagagccgtc agaatctttt tcaattgtgc
     2041 ataactaaga tttactttgt acattaccct agttttattg gctccgccat ttgcagcctt
     2101 tagaatctgg tttattatgt caattctgtc tctgtttatc atacgcctct tagtttccca
     2161 aaaaggggta aaggatataa gtatatcatt ataatggaac gcctgatata caaataaact
     2221 ctaatgaaga agtattataa tttgtatatt attttttcta gtagttgaga agatacaagt
     2281 cactgtttaa aaggctggta gttgataaga gccgactcaa gcaactctat cttatcatat
     2341 gatccattat tatatgactg aagcttgtcc accaaattct gcaaaagcga ggacacctgt
     2401 aatgctgtac ttgaaaggct cttagaccat tgggataaga taaaggctaa ggaa
//
LOCUS       DASQTF010000164         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1166884,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000164 DASQTF010000000
VERSION     DASQTF010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166884"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VE308_03870"
     CDS             <1..1053
                     /locus_tag="VE308_03870"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZB00143.1"
                     /translation="VGEQKTKPTQHSVQELRRIGIQPDILAVRCKMPLRDDARRKISL
                     FASVEQNCVISCHDAPSIYKVPEVLENQGMLRSIAERLGLQRTMPTWGNWKAIAESLS
                     KYEGSIKIAVVGKYVKLPDSYVSIYHALLHAGASIGKKVDVYWIDSEKFENGSQQDLS
                     MLKNFEGILAPGGFGIRGSEGIISAANFARANDIPYLGICFGFQLAIVAFARHVCKLS
                     DANSTELEPNTKNPVVDFMPEQLDVHNMGGTMRLGSHEITITPKTFAAGIYRSTSIRR
                     RHRHRFEFNQKYLDIVTKNGLVMSANSDRGRRMEMLEILNHKFFFAVQYHAEFNSRPG
                     KPEQAFEAFIRAAAKY"
     gene            complement(1050..2120)
                     /locus_tag="VE308_03875"
     CDS             complement(1050..2120)
                     /locus_tag="VE308_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZB00144.1"
                     /translation="MDFSVFLNFKFGCGTREYSIITMKVALSGIDIQDGALLSKVKKT
                     LESLTVKTQIMKDDDFHAAKDVDLVMVAGGDRGILDYFHRIEYDSAPVLGIYETDSTG
                     FLAQLDVRDLESATSQLKRGGYSVEEVFRLAVKVDGKQADPVLNDVAVFPSKSATLME
                     HVLRIDANDIWHDKSDGVIISTPTGSTAYSMSAGGPMVLQKALVFLIVSVNSLDNTRR
                     PLIISNQSKLEITDIISRYHCEVVLDGGHRIQIKNTLECSKHAFPARLVRLGTHSSAA
                     SIMAKKVKLAEDMLKMPPSAKLILKTLEYEGSLSQKDLAARTMLPERTIRLSLSHLIN
                     QGYVKKKTSLRDARQRIYELKF"
BASE COUNT          624 a          469 c          528 g          601 t
ORIGIN      
        1 gtgggcgagc aaaagaccaa gccaacacag catagtgtcc aggagctaag aaggattggt
       61 atccagccag atatccttgc tgtcagatgc aagatgccac taagagatga tgcaaggcgc
      121 aagatttcgc tttttgcaag cgtagagcaa aattgcgtaa tctcgtgtca tgatgcacct
      181 tcaatataca aggtaccgga agtgcttgaa aaccaaggta tgctcagatc aatagcagag
      241 cgacttggac ttcaaagaac tatgcctaca tgggggaact ggaaggccat agcagaatct
      301 ttgtcaaagt atgaaggttc tatcaaaatt gcagttgtag gcaagtacgt aaagctgcca
      361 gacagctacg taagcatata ccatgcacta ttgcacgctg gtgcaagcat tggcaaaaag
      421 gtcgatgtct actggatcga ctctgaaaaa tttgaaaatg gtagtcagca agatctatca
      481 atgttgaaaa actttgaggg gatactggca cctggtggct ttggaataag aggaagtgag
      541 ggaatcataa gtgcggccaa ctttgcaagg gcaaacgaca ttccatatct tggaatctgc
      601 tttgggttcc agctggcaat tgttgcattt gcaagacatg tatgtaaatt gagtgatgca
      661 aattctactg agcttgagcc aaatacaaag aatcctgtcg ttgatttcat gccagagcag
      721 ctcgacgtcc acaatatggg aggcacgatg cggcttggaa gccatgaaat aactattact
      781 ccaaaaacat ttgcagccgg tatatatcga tcgacctcaa taaggaggcg acacaggcac
      841 cgatttgaat ttaatcaaaa gtaccttgat atagtaacaa agaatgggct tgtgatgtca
      901 gctaattcgg acaggggcag acgaatggaa atgctggaaa tactgaatca taagtttttc
      961 tttgctgtac aataccacgc ggaatttaac agtaggccag gtaagcctga gcaggccttt
     1021 gaggcattta tcagggctgc agcaaagtac tagaatttta attcgtatat cctctgtctg
     1081 gcatcccgga gggaggtttt cttttttacg tatccttggt tgataaggtg gctaagagaa
     1141 agtcgaatgg tgcgctcagg gagcatagtc cgcgcagcta ggtccttctg gcttaatgaa
     1201 ccttcatact caagagtctt cagtattaat ttggcacttg gaggcatctt tagcatatcc
     1261 tcggcaagct taactttctt tgccataatt gatgccgcag aagaatgagt gccaagcctt
     1321 acgaggcgtg cggggaatgc atgcttgctg cactcaagag tgttttttat ctgtatcctg
     1381 tgacccccat cgagcactac ttcgcagtgg tagcgactta tgatgtcagt tatttcgagc
     1441 ttgctttggt ttgatataat aagtggcctg cgggtgttgt caagtgagtt tacagaaacg
     1501 atcaaaaata caagtgcttt ttgtagcacc atgggtccgc ctgcagacat tgaatatgca
     1561 gtcgagccag taggagttga aattatgacg ccgtcgctct tgtcatgcca aatgtcgttt
     1621 gcatctatcc tcaggacatg ctccatgaga gtcgcactct tgcttggaaa aactgctaca
     1681 tcatttaaga cagggtcagc ttgcttaccg tctactttaa cagcaagtcg aaaaacctcc
     1741 tcaactgaat agccgccgcg cttcagctga gatgtcgcag actctaaatc tcttacatct
     1801 agctgggcaa ggaagcctgt tgagtcagtc tcgtagatgc caagaacagg agcggagtca
     1861 tactcgatcc tatggaagta atcaagtata cctctgtctc ctccggccac cattaccaga
     1921 tctacatcct ttgcagcatg aaagtcatcg tccttcatta tctgggtctt gacggtcagt
     1981 gattcaagcg tctttttcac tttggaaagg agcgctccgt cttgtatatc gatgcctgaa
     2041 agcgcaactt tcattgttat tatactatat tctcttgttc cacagccgaa tttaaagtta
     2101 agaaagacgc tgaaatccaa tatcattgta aaaataggac gccgtctgtt ctatcttcga
     2161 catggaggga attttggcat ggccgacttg ccttgtttca agagctgcct taactgcacc
     2221 gg
//
LOCUS       DASQTF010000165         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_422927,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000165 DASQTF010000000
VERSION     DASQTF010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422927"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="coaBC"
                     /locus_tag="VE308_03880"
     CDS             <1..942
                     /gene="coaBC"
                     /locus_tag="VE308_03880"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZB00145.1"
                     /translation="ANTLGKVAAGIDDTPVTSILSVALGSKIPIIVAPAMHEAMYDNI
                     FVQQNVSKLREQMVFIEPKMEGGKAKVADPEQVLNATISVLSNRALLSGKRVLVTAGS
                     TIEYIDPIRVMTNLSSGKMGIAIAQEAQRMGATVTLVHGHGTLSSTSDKIVQVNTGEE
                     MYKAIVSELSSRRYDIVVMAAAVADFAPAKKSYQKIDTKLGKMELSLVATRKIIDEVK
                     NKSKDAFLVAFKADYGVSDSVLTEKAYRKLKECDANIIVANDLGRKGSEPGSDKNEVF
                     IVDRSKKIVHLPLESKAQVARRLLELVAELTAESHSK"
     gene            complement(1246..2295)
                     /locus_tag="VE308_03885"
     CDS             complement(1246..2295)
                     /locus_tag="VE308_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZB00146.1"
                     /translation="MQHAQEAEKCNVVAAFEPENVFYLTGFWGEAIAVCMDNGITKLI
                     TPKLEYSRAEKTSIDCEVLATERGGELISTFVSHIKGSRVCSDSSDYYTIESILKRGG
                     DILVNTDPFFLTRRIKDQAEIKVISKAAKILDKLYEICEEEIKVGLSERDLQAKLIYE
                     AMKMGANPPSYKSTLNPLIIAGGPNSALPHAEVTDRKFRKADMIVVDLTLRHAGYIAD
                     ATRTFVLGSATKKMKEVYGIVQEAQKIALNRANAGITCGQVDAACRNLIAERGYERLF
                     IHSTGHGIGLDVHEPPWLKMKNDEELRANMAVTVEPGIYIENKFGVRIEDSIIVNNGK
                     PQVLNRFTKELLVLR"
     gene            2431..>2893
                     /locus_tag="VE308_03890"
     CDS             2431..>2893
                     /locus_tag="VE308_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZB00147.1"
                     /translation="MEYLVMLPGPTNVPNRVMNAMLAPMINHRSDDFRTLYKAIVEKT
                     QKVFQTSNDIVLLTTSGTGAVEASVVNLIRKGDKAVIPVNGEFSTRLADLIDSWGGEA
                     IRISSTPGENPPYDKFEETFEKHKDIKAIYAVYNETSTGTTIRYMDKLGELA"
BASE COUNT          804 a          645 c          641 g          803 t
ORIGIN      
        1 gccaacaccc ttggcaaggt agcagcgggc atcgatgata cacctgttac atcaattctt
       61 agcgttgcac ttggcagcaa gatcccaata atagtagctc ctgcaatgca tgaggccatg
      121 tatgataaca tatttgtcca acaaaatgtc agcaaattga gagagcagat ggtattcatt
      181 gaacccaaga tggaaggagg caaggcaaaa gtggctgacc cggagcaggt actaaatgcc
      241 acaatttctg tcctttccaa tagggctctt ttgtcaggca aaagagtgct tgttacggct
      301 ggtagtacca ttgagtatat tgacccaata agggtcatga ctaacctaag ctctggcaag
      361 atgggtattg ccatagcaca ggaggcacag cggatgggcg caactgtgac gctggtgcat
      421 ggtcacggaa ctctaagctc aacaagcgac aaaatagtgc aggtaaatac aggtgaggaa
      481 atgtataagg caattgtttc tgagttatca tccaggaggt acgatattgt tgtaatggct
      541 gctgcagttg cagattttgc acctgcaaaa aaatcatacc aaaagatcga cacaaagcta
      601 ggtaaaatgg aactgtcgct tgtagctaca aggaaaataa ttgatgaagt gaaaaataaa
      661 agcaaggacg cattcctcgt tgcattcaag gcagactacg gtgtatcaga ctcagtcctg
      721 acagaaaagg catatagaaa actgaaggag tgtgatgcca atattattgt ggcaaatgac
      781 cttggcagaa aaggttcaga gcccggctct gataaaaacg aagtgtttat tgttgacagg
      841 agcaaaaaaa ttgtccactt gcctcttgag agcaaagcac aagttgcaag aagactccta
      901 gagcttgtgg ctgaattaac agcagagagt catagcaaat aatgctgtcg caataatagc
      961 gaccacatgg aatttttgga atacttagta ttgccgccat actaaatcct acttgccata
     1021 tcagcaatct caaatcctgt tccctgtccg ccgtatgatg tcaggctcga tattatcgaa
     1081 aatttcggtc aaaaacactt tttgatctgg acgtcaggaa ttgtgcaaat gatctttttg
     1141 ctgctctgta atcagttcta ggaactggca aagatcgcca aggtggcaag aatcgtaatg
     1201 atcctgacca tcatccattg ttcaagtcga actagctttt ttatttcatc tcagaacaag
     1261 aagctctttt gtgaatctgt tgagcacctg cggcttgcca ttatttacga ttatgctgtc
     1321 ctctatcctc acgccgaatt tattctcaat atatataccc ggctcaacgg tcacagccat
     1381 atttgctctg agctcttcgt catttttcat cttaagccaa ggaggctcgt gaacgtcaag
     1441 acctattcca tggccggttg aatggataaa tagtctctca tagccgcgct ctgctatcaa
     1501 gtttctgcat gccgcatcaa cctggccaca tgttatacct gcatttgccc tgttgagggc
     1561 tattttttgc gcctcttgca ctatgccgta cacttccttc atctttttgg ttgcagagcc
     1621 aaggacaaat gtccttgtag catcagctat gtagccggca tgccggagtg taaggtcgac
     1681 cactatcata tccgccttgc ggaattttct gtctgttact tcagcgtgcg gcagcgcgct
     1741 attgggcccc cctgctatga tcagtggatt cagcgtagac ttgtatgatg gaggatttgc
     1801 acccattttc attgcttcgt agatgagctt tgcctgcaga tcgcgctctg acagacctac
     1861 ctttatctcc tcttcgcata tttcatacag cttatccagt atcttggcgg cttttgagat
     1921 aactttgatc tctgcttggt cctttatcct gcgtgttaga aagaatggat ctgtattgac
     1981 caaaatgtcg ccgcctcgtt tgagaatcga ctcgatagta tagtaatcgc tgctgtcgct
     2041 gcacactctg ctacctttta tgtgtgaaac aaatgtggaa atgagttcac cccccctttc
     2101 agtggcaagt acctcacagt ctattgaagt cttttcggcc cgagaatact ctaatttagg
     2161 cgtgatcaac tttgttatgc cattgtccat gcatactgca atggcttcgc cccaaaaccc
     2221 tgtgagatag aatacgtttt ctggctcaaa tgctgcaacc acattgcact tctctgcttc
     2281 ctgggcatgt tgcaaaacag tataacggcg ctccttcatc gacaagtccg aagagttaca
     2341 cactctctta gttttataaa gcgttatgat gctgtaatta ttgtaatttg atttaaatat
     2401 ggctggctct cccgcttttt ttgagactat atggaatacc tggtgatgct ccccggtcca
     2461 acaaatgtcc cgaatagagt catgaacgca atgctcgcgc ctatgataaa ccacaggagc
     2521 gatgactttc gcacattata caaagcgata gttgaaaaaa cccagaaggt ctttcagact
     2581 tcaaatgaca ttgtgcttct tactacttct ggcaccggtg ctgtcgaagc gtctgttgtt
     2641 aatcttatta ggaaaggcga caaggcagta atcccagtca atggtgagtt tagcacgaga
     2701 cttgcggatc ttattgatag ctggggcgga gaggcgatca ggataagctc aacgcctgga
     2761 gagaacccac cttacgacaa gttcgaggaa acatttgaaa aacacaaaga cattaaagcc
     2821 atttatgcag tttataacga aacatccact ggaactacaa taaggtacat ggacaagctg
     2881 ggtgaacttg cgg
//
LOCUS       DASQTF010000166         6660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_699615,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000166 DASQTF010000000
VERSION     DASQTF010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699615"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VE308_03895"
     CDS             complement(<1..568)
                     /locus_tag="VE308_03895"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HZB00148.1"
                     /translation="MNNQEILFSDSILTLGGLDIGGSVSKCLAPKLDVLKGKKIVFCY
                     DDKKMMPVDGWGMRSDPYNTTTVTTIRPIKENALVAAIDSSSIKLAETEDGSLYAIKC
                     SVATAYASHTLMHFKIGPVLFYLSEKTVHESELEERLSRLVLLDDDLAKRLIRVRVER
                     AVQKEIASHFTNSIILVDGSLKASIFEER"
     gene            678..1175
                     /locus_tag="VE308_03900"
     CDS             678..1175
                     /locus_tag="VE308_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00149.1"
                     /translation="MSGHAAEEVKIRLKHGEWEVEVVCIESRVKQVVESVLSSLDATK
                     MIGPEVQSQLDALRREIDSLKTRPITELVPEGQQQQKVTTKSGMTCRGLLENLWFEGH
                     FVSERSLGEVHEELSRRGYNYDRTAVSHSLTDMVRENILTRTGAMRNYRYIQKRPPNS
                     TTAQA"
     gene            complement(1159..1674)
                     /locus_tag="VE308_03905"
     CDS             complement(1159..1674)
                     /locus_tag="VE308_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00150.1"
                     /translation="MSQAENDVNVTVTIGDVKVEFSGSAETITASVMNFLLKEVPSMD
                     LARKILLNYAAQELIETYSHLIKITPEGPRVILDTDVKLSDKDIVALQLVAVRIAKDL
                     GRLQDSSMQVSDIHTATALNPKSISSRISEMVKAGHVARDEKEPSKYRITTAGIHWLN
                     STIPKKIRPGQ"
     gene            complement(1706..2359)
                     /locus_tag="VE308_03910"
     CDS             complement(1706..2359)
                     /locus_tag="VE308_03910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZB00151.1"
                     /translation="MPRLAGRFTEEKADFHPRSWLSKYRRTSVGYLTKMLLFYHGVGI
                     LLLLAVTPLIEQVLPDYEQPSIPRSLVSVLAAGPLEETIFFGIPFYIFGNAYSVLVTG
                     TVWVGIHLLNTDTLSVNSLAFGNLLFVLPSLFFSLRTWVSGKGWFSVITHSAWNSIFF
                     TAGCSVGEFTCTAIERDISATVISIALSGVLLGVTYLLYKRRENKERQWSQSNEFNI"
     gene            complement(2399..2722)
                     /locus_tag="VE308_03915"
     CDS             complement(2399..2722)
                     /locus_tag="VE308_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00152.1"
                     /translation="MYKPKSTREIYIESLSINSESIEKQLADQSVPSEEIRSILDFVS
                     KMYYENVERIVTLCDKDMMALEYVPSPLKLFVDCLASAQKSLSLSSPASRLIQRYTSA
                     WEDWM"
     gene            complement(2725..4767)
                     /gene="gatE"
                     /locus_tag="VE308_03920"
     CDS             complement(2725..4767)
                     /gene="gatE"
                     /locus_tag="VE308_03920"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HZB00153.1"
                     /translation="MPSPLDHEHLNLKVGFEIHQQLATKRKLFCNCSCKEAKEYDSSF
                     VFVRRLRPTQSELGAYDPAAIFEFSKMHTVEYHAPPGSSCLVEADEEPPHEVGPEALE
                     TALIFSLKLHSKVKDEIHVMRKIVIDGSNTTGFQRTMLVASGGYLDVAGKQVGVQSVS
                     LEEDAAKLIRDEKGVRKFGLDRLGVPLVEIALEPVTGRPSEIMQVALTLGRLLRASKR
                     VARGLGSIRQDINISVRDGAVVEVKGVQKLDQLVKVIEYEMHRQYGLILIAQKLKEKN
                     IDVKKVDDRIEDVSDIFGNKASSRIVKKILEGGGRILAIKVPGFAGMIGFEPYTDIRL
                     GRELGKLVKFYDIDGIFHSDELPNYGITEDDVVAVKQRLQMNDNDAFIMIGGLNDKIK
                     FASEAIILRLKAAINGVPAETRAATSDGKTVFLRPRPGIARMYPETDILPITTTKPML
                     EPLADKVPPHWDEIVDSLTKKYDLNKELASQIFDSDYFNMFEEIASETKIEPTFIASK
                     LTEGLTSLERQGLDVSVLTSNDIKDVFKELDKGSTHKEAVILLFEELIKAKSRPNCDN
                     CKIGKMRPELARVTDQIRVYKCDNCGKRDMVQVVNEQLKISEVIRAVGVSSITDEELS
                     KGLDRIMSNNMAIIKEKGMNALSTLMGRAMAEYRGKANGQKVNAMLKDKMSKMVNK"
     gene            complement(5185..6537)
                     /gene="gatD"
                     /locus_tag="VE308_03925"
     CDS             complement(5185..6537)
                     /gene="gatD"
                     /locus_tag="VE308_03925"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HZB00154.1"
                     /translation="MKALTKYDNSGGYGEEAGLKLLSKHNISVGDSIKITTEDDDEMT
                     GLLMPRYESASKEYIVIKLKSGYNTGIHVGKIKSIAKVTATLTARYDITPAVTTKQNL
                     PKVALISTGGTIASKIDYRTGGVHAALSADELYASVPELADHASIDPEVLMSEYSENL
                     RPEHWTLIAEKVAEKIKTNKYRGIVVSHGTDTMHYTAAALSFALQKLPVPVVLVGAQR
                     SSDRPSSDAALNLLGATIFAAESKYPGVFVAMHGGTSDDIIACHAGTRVRKNHTSKRD
                     AFESIDMTPVAIVRNNKIEMQQKDQNRLGETFSDKSFRVNTKFENRVMLLKYYPGFDP
                     TLIEHAVNAGYRAIVLEGTGLGHVGKDCFPALKKAVNVGLIVCMTSQCIWGRVGMTVY
                     NTGRDLLEIGVIPLSDMISETATVKAMWALANSKNVEDAKKIMQENLANEISDFIPIL
                     "
BASE COUNT         1692 a         1596 c         1381 g         1991 t
ORIGIN      
        1 ctctctcttc aaagatcgat gctttgagcg agccgtcaac taagattatt gaatttgtaa
       61 agtggcttgc aatctctttc tggacggctc tctccacgcg tactcttatc agacgcttcg
      121 ccaagtcatc atccaagaga acaagtcttg agagacgctc ttccagctcc gattcatgaa
      181 ctgtcttttc gcttaggtag aacagcactg gccctatctt gaaatgcatg agagtgtggc
      241 tagcataggc cgtagcgacg ctacatttta tggcgtaaag gcttccatct tctgtctcgg
      301 ctagcttgat actgctggag tctatggccg ccacgagcgc attctctttg atcgggcgaa
      361 tggtagttac cgttgttgta ttgtaggggt cagatctcat gccccatcca tcaactggca
      421 tcattttctt gtcgtcatag caaaacacaa ttttcttccc cttcagtacg tcaagctttg
      481 gagcaagaca tttgctcact gatcccccta tgtcgagacc ccctagagtc aagatgctgt
      541 ctgagaaaag gatttcttgg ttgttcatgt tgatcaacaa tatctgcggg cacaagtata
      601 ttaatattgc tcaacatgat caatatggcc agcaacagat ttatcaatag cagcttgaaa
      661 ggtatattta agcaaatatg agcggccatg ctgctgagga agtcaaaata aggcttaagc
      721 acggtgaatg ggaggtagag gtagtctgca ttgagagcag agtcaagcag gtagttgaaa
      781 gtgtgctctc aagccttgac gctaccaaga tgatagggcc agaagttcag agccagctcg
      841 atgcactgag gcgagaaata gactccttaa agactaggcc tattactgag ctagtaccgg
      901 agggccagca acaacagaag gtgacaacca agagtggtat gacctgtaga ggccttttgg
      961 aaaatctctg gtttgagggg cattttgtta gtgaaaggtc gcttggtgaa gtacatgaag
     1021 agctctcaag aagaggctac aattatgaca ggactgcagt ctcacattcg cttacagata
     1081 tggtgcgaga aaacatcttg acaagaaccg gagccatgcg gaattaccgc tacatccaga
     1141 aaaggccacc aaacagcact actgcccagg cctgatcttt ttgggtatcg ttgaattgag
     1201 ccagtggatg cctgctgtcg ttatcctgta tttgctcggc tctttttcgt cacgtgcgac
     1261 gtggccagcc ttaaccattt cagatattct tgagcttatg gactttggat tgagcgccgt
     1321 agcagtatga atatctgata cctgcataga gctgtcctgc agcctgccaa gatcctttgc
     1381 aattctgaca gctacaagct gcagagctac tatatcttta tcagacaact tgacatcggt
     1441 atctagaatt acacgcggac cctctggtgt gatcttgatg agatgggaat acgtttctat
     1501 gagctcttgc gctgcatagt tgagtaggat ctttcttgca agatccattg aaggcacctc
     1561 cttaagcaga aagttcatca cagaagctgt gatagtctcc gcagagccgc tgaactcaac
     1621 ctttacgtca cctattgtta cggtgacgtt aacatcattt tcagcttggc tcattattgt
     1681 tttcgcttgt aacacaaatg gcgggctata tattgaattc gttagactgc gaccactggc
     1741 gctccttgtt ctcccttctc ttatagagta gataggttac tccaagcaat acacccgaca
     1801 gtgcaatgct tatcactgtg gcgctaatgt ctctctcaat tgctgtgcac gtaaattcac
     1861 cgacagagca gccagcggta aagaagatac tattccaagc agaatgtgta atcactgaaa
     1921 accaaccctt gccactgacc catgtcctta ggctaaagaa tagggatgga agcacaaaga
     1981 gcaagttgcc aaacgcaagg ctgttgacgg aaagcgtatc cgtgttcaaa agatgtatcc
     2041 cgacccacac ggtcccagtg acaaggacag agtaggcatt tccaaatatg tagaacggga
     2101 tgccaaagaa tatcgtctct tctaatggtc ctgcggcaag tactgacact agtgaccttg
     2161 ggatgcttgg ctgctcatag tctggaagaa cctgctcaat cagaggtgtg actgcaagaa
     2221 gcagaagaat gccaaccccg tggtaaaaca acaacatctt tgtgaggtag cctactgaag
     2281 tgcgcctgta cttggacaac cacgatctag gatgaaagtc cgccttctct tcagtaaatc
     2341 tccctgccaa ccggggcata gcataactgt tacactaaat aaggttctgc ctgtctggct
     2401 acatccaatc ttcccatgct gatgtatacc gttggatcag cctgcttgca ggtgacgaca
     2461 agctaagcga tttttgcgca ctggcaagac aatcaacgaa tagcttgagc gggcttggca
     2521 catactcaag agccatcatg tccttatcgc agagcgtgac aatcctctca acattttcgt
     2581 aatacatctt tgaaacgaaa tctagtatgc ttctaatctc ctccgatgga actgactggt
     2641 cagcgagctg cttttctatg ctttcagaat tgatggaaag ggattcaatg taaatttccc
     2701 tcgtagactt gggtttatac attattactt gttgaccatc ttgctcatct tgtccttcag
     2761 catagcattt actttttgac cgttggcttt gcccctgtat tctgccatcg cccttcccat
     2821 gagtgtgctc aacgcgttca tgcctttttc tttgattatt gccatgttgt tgctcattat
     2881 cctatcaagt cctttgctca gctcttcatc agttattgaa gacacgccta ccgcccgaat
     2941 gacttcactt atttttagtt gttcattaac aacctgcacc atgtctcgct tgccgcagtt
     3001 gtcgcatttg tatactcgaa tttggtcagt cactcttgcg agctccggcc gcattttccc
     3061 aattttgcag ttgtcacaat tgggtcgact ttttgctttt atcagctctt caaatagcaa
     3121 gataaccgct tctttatgtg tggagccctt gtcaagctcc ttaaagacgt cttttatatc
     3181 gttacttgtc aagacagaaa catccaatcc ttgcctttcc aagctggtta gaccctctgt
     3241 caacttggat gcgataaatg tcggctctat cttggtctcg cttgcaatct cctcaaacat
     3301 gttaaagtag tcagagtcaa atatctgact tgccagctcc ttattgagat catatttctt
     3361 agtaagcgaa tccacaatct catcccaatg tggcggcact ttgtctgcaa gtggttcgag
     3421 catcggctta gtggtcgtaa taggcagtat gtctgtctca gggtacatcc ttgctatacc
     3481 tggccttggc cttaagaaca ccgtcttgcc gtcagaggtt gctgcgcgag tttcagcagg
     3541 aacaccatta attgcagctt taagccgcag aattattgcc tcagaggcaa acttgatttt
     3601 gtcatttaga cctcctatca taatgaaagc atcattgtca ttcatttgta gtctttgttt
     3661 gactgctaca acatcatcct ctgttattcc ataatttgga agttcgtctg aatggaagat
     3721 accatctatg tcataaaatt tgactagctt gccgagctct cttccaagtc tgatgtcagt
     3781 atagggctca aagccgatca tgcctgcaaa ccctggcacc tttatcgcta ggatgcgccc
     3841 tccgccttca agaatctttt tgacaattct tgaagatgct ttgttgccaa aaatgtcaga
     3901 gacatcttca attctgtcgt ctactttttt gacatcaata ttcttttctt tcaatttctg
     3961 tgcgatcaat attaagccgt attgtctgtg catctcatac tcgattactt ttaccaactg
     4021 atcaagcttc tgcacaccct tgacctcaac tacagcaccg tcacgcaccg agatgttaat
     4081 gtcctgcctg atgctgccaa gcccccgtgc aacccgtttg ctcgcccgaa ggagcctgcc
     4141 tagcgtaagg gcaacctgca tgatttcgct aggtctaccc gtcacaggct ccagtgcaat
     4201 ttccacaagt ggcacgccca gcctgtcaag gccgaacttg cgcactcctt tttcatccct
     4261 tattaattta gctgcatctt cttcaagact gacgctttga actccaactt gcttacctgc
     4321 aacatcaaga tagccaccag acgcaacaag cattgtacgc tgaaaaccag tggtatttga
     4381 gccgtctatt actattttac gcatgacatg aatctcatcc ttgaccttag aatgaagttt
     4441 aagtgaaaat attagcgcag tttcaagtgc ctctggtccg acctcgtgcg gaggttcctc
     4501 gtccgcctct acaaggcagc tagaaccagg aggcgcgtga tattcaacag tgtgcatttt
     4561 ggaaaactca aatattgccg cagggtcata tgccccaagt tcactctggg ttggccgcag
     4621 cctgcgtaca aatacaaatg aactatcata ttcttttgct tccttgcagc tgcagttgca
     4681 gaagagcttt ctctttgtgg ctagctgctg gtgtatttca aagccgacct tcaggttcaa
     4741 atgttcatga tctagaggag acggcactgg atagaaattt ccttacacac gtattaagcc
     4801 ttccagtcac gttggatcac cttattaaaa ctcaatttgc ttgcatccct ttcaggatta
     4861 gtggtttgtg atatcgcatt cctgtacatt gcttcacctt agagcctttt tgtactagga
     4921 aactaagtca tctatttaag acgagctcaa aaccctttca ggatgtgaga atagtttctg
     4981 ttacctcgga gagattacta acttccattc cttgaaaata tctatatcct gacatcgtta
     5041 ccaataatat agtttatctt cagaaggcat gctatgtatg catcttataa cagcatggtc
     5101 tcttgttcgt tgcactatct ttgcggcttt gacgttcggc atagaatgga aaagttgctt
     5161 tgcagtcata atttatttta ttcatcataa tatcgggatg aagtctgaaa tttcatttgc
     5221 aaggttttct tgcattattt ttttggcatc ttccacattc ttgctgtttg ccagcgccca
     5281 cattgctttc actgtagcag tctcagaaat catatcagat agaggtatca cgcctatttc
     5341 gagtaagtct ctgccagtat tatagactgt catccctacg cgcccccata tacactgtga
     5401 tgtcatgcat actatgaggc ctacgttaac tgcctttttg agagcaggga agcaatcctt
     5461 gcctacgtga cccagtcctg ttccctcgag aactatcgcc ctgtagcctg cattcactgc
     5521 atgctcgatg agcgtaggat caaagccagg atagtatttc agcaacatga ctctattctc
     5581 aaattttgtg tttactctaa aagacttgtc gctgaatgtt tccccgagcc tgttctggtc
     5641 tttttgctgc atttctattt tgttatttct tacaatggct acaggagtca tgtcaattga
     5701 ctcgaaagca tcacgcttgc ttgtgtggtt ctttcttact cttgtcccgg catggcatgc
     5761 aataatgtcg tctgaagtcc caccatgcat cgcaacaaat acaccagggt attttgactc
     5821 tgctgcaaat atcgttgcac caagcagatt caatgcagca tcagaagacg gcctatctga
     5881 tgatcgctgc gcaccgacaa gcaccactgg aaccggcaac ttttggagtg caaagcttag
     5941 agcagctgca gtataatgca tagtatcagt tccatgagaa accacaatgc ctctatactt
     6001 gtttgtcttg attttttctg cgaccttttc tgctatcaaa gtccagtgtt ctggcctgag
     6061 gttctcactg tactcactca tcaaaacttc aggatcaatt gatgcatgat cggcaagctc
     6121 aggcaccgat gcatacaatt catcagcaga aagggccgcg tgcacgccac cggtgcggta
     6181 atctatcttg cttgctatag tcccgccagt gcttatcaag gcaacttttg gcaaattttg
     6241 tttagtagta actgcaggag ttatgtcata gcgagcagtt aatgtagcag ttacttttgc
     6301 tatcgatttt atttttccga catgtatgcc cgtattatat ccgcttttta attttatgac
     6361 aatgtattct ttactagcag attcatagcg cggcatcaac aggccagtca tctcgtcatc
     6421 atcctctgta gtaatcttga tgctgtcgcc aactgaaata ttgtgctttg atagtagctt
     6481 aaggcctgct tcttccccat agccaccact attgtcatac tttgtcaatg ctttcaaagt
     6541 caattgctaa atatattaac aaatctttct tagctaaaga gtgtccgggc tccatccact
     6601 atgaattgga cagcaatagc agcgatgatc actgcaaaaa ccctagtaac aataattgag
//
LOCUS       DASQTF010000167         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_873454,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000167 DASQTF010000000
VERSION     DASQTF010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873454"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VE308_03930"
     CDS             <1..1014
                     /locus_tag="VE308_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZB00155.1"
                     /translation="KKVVKIEDIDTPKPKANEVLIKVEAAAYNYNDLWGIWGEPIKIP
                     MPHISGSDIAGTVVELGESVTGKINVGDRVASYPNLTCRVCYECTSGREYDCGSRQVW
                     GFQTGPLWGGFAQYTHLSEVNVVKLPDNVSFTDAAAISMVGMTAWHMLVTRAKIRPGQ
                     TVLIMGGGSGMGIAGIQIAKLFNCDVIATAGNKDKMDKCLELGADNVVNHRESDWYKK
                     VREITNSQGVDVVYEHIGKSVFPQEVGLLKMGGTLVSTGATTGYDSTIDLRYLFFKGI
                     NLLGATQGTKAGLEEVIGWVSKGKIKPVIDTILPFDNMVEGHVKMADSQLFGKIVTTP
                     QTL"
     gene            complement(1186..1470)
                     /locus_tag="VE308_03935"
     CDS             complement(1186..1470)
                     /locus_tag="VE308_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZB00156.1"
                     /translation="MRLGLPQGRRNVWLHEWPAAWRPDQNFHDFVEKNGTAPDIKTRV
                     DVLAITPTTAVVRVDMERDVIGGDYTDFHTLIKLNGTWHIISKVYHMYGG"
     gene            1605..3425
                     /locus_tag="VE308_03940"
     CDS             1605..3425
                     /locus_tag="VE308_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZB00157.1"
                     /translation="MASKKNDCKISYRSYDVLIIGGGSAGLRAAIEAHDAGSEVLIVS
                     KNRRGDPHTVLARGGINAALGTMDSQDNWMIHAADTLSEGLFIADYDKVEMLCKSAPD
                     AMTELVKWGARFHREPDGRLTQRFFGAHTYRRTCFYGDWTGKEIIRVLMKQVRLRKIK
                     FIDNVYITKLLKSTNIEKTQGAQGKGKEQEISGAMGIDLKRKKIIIFKAKCIILATGG
                     YTRVYPVSSSRIFENYGDGVALAYEAGVDLVDMEMVQFHPTGMVWPKKAVGTLATEAI
                     RGEGGILLNSKGERFMKSYYPQRMELGPRDVVARSIYNEIMSGRGTKHGGIWLDITHL
                     PKAKILERLPSMYDQFKRIAGVDISKEKMEVAPTAHYSMGGVKVDLKCQTNVRGLFAI
                     GEVAGQVHGANRLGGNSLLGTVVFGKIAGREAASKIAREKKRTTNGRSSTSSILGITY
                     NEDKRLAYTLFAVKQPLRILRQVQQVMQENSGIIREGIKLRKAIKKILELEKTFYSKH
                     SVLKSYTDYENVVNTWEVKSALVVCEAILRSALMRQESRGAHYRSDFPERDDSKWKVN
                     IHCTKSGEEMVMFKLPVSKLREPIEELLKTEMKVEHHLLE"
BASE COUNT         1124 a          654 c          883 g          891 t
ORIGIN      
        1 aagaaagtag ttaaaatcga agatattgat acaccaaagc caaaagccaa tgaggttttg
       61 ataaaagttg aagcggcagc atacaactat aatgatctct gggggatttg gggtgaacca
      121 atcaagattc caatgcctca catatcagga agcgacattg ctggaacagt tgttgaactt
      181 ggcgagagcg tcacaggaaa gatcaacgtg ggagataggg tggcctcgta ccccaacctg
      241 acttgcaggg tatgctatga atgcacttct ggaagggaat atgattgcgg cagcaggcaa
      301 gtatggggtt ttcagacagg tccgctctgg ggcggttttg cgcagtacac acatctatca
      361 gaggtcaacg tggtaaaact tccggataat gtatcgttta ctgatgcagc ggcaatttca
      421 atggtaggca tgacagcctg gcacatgctt gttacccgag caaagataag gccaggtcag
      481 acagttctga ttatgggagg agggagtgga atgggaatag caggtataca aattgccaaa
      541 ttattcaact gtgatgtcat tgccactgca ggaaacaagg acaagatgga caagtgcttg
      601 gagcttggtg cagacaatgt agtaaatcat cgtgaatcag attggtacaa gaaggtacgc
      661 gaaataacaa atagccaagg agtagatgtg gtctatgagc acattggcaa gagtgtgttt
      721 ccacaagaag ttgggctatt gaagatgggt gggacgcttg tgtctactgg cgcgacgact
      781 ggttacgact ctactattga cctccgatat cttttcttca aagggatcaa tctgcttggg
      841 gcaacgcagg gaacaaaggc cggcctggaa gaagtaatag gctgggtcag caagggaaag
      901 ataaagccag tgattgatac aatccttcca ttcgacaaca tggttgaagg tcacgtcaaa
      961 atggccgact cccagttgtt tggcaagata gttacgactc cacaaacact ataagagctg
     1021 ttcagaaatg agggatttca gatttagaac agcaataaac tgcttctgct aaccaactta
     1081 gcaaacgata taagtttact caaaatccag attatgtctt catgcagtag caaatactgt
     1141 catttcttag aagatgggtt attaaacgag gttgcaacat gccctttacc ctccgtacat
     1201 gtgataaacc ttggagatga tgtgccaggt gccattaagc ttgatgagtg tgtggaagtc
     1261 agtgtagtcg ccgccgatca cgtccctctc catatcgacg cgcaccactg ccgtcgttgg
     1321 cgttatggcg agcacgtcga ctcgcgtttt gatgtctggc gcagtgccgt ttttctcgac
     1381 aaagtcatgg aagttctgat cgggccgcca agcagctggc cattcgtgaa gccatacatt
     1441 acggcgtcct tgtggaaggc ctagtctcat ttgtcaaata gccgcctaat cgaattacac
     1501 tacgatatat ctataataat aaaatggagg tcattagctc ctagtgctaa ccaatgtcta
     1561 tacaggtaac aataaagtag tttaattgta ctacgagatc atgtatggca agtaagaaaa
     1621 atgactgcaa aatatcttat cgctcttatg atgtccttat tataggtgga ggctctgcag
     1681 gcttgcgtgc tgcaatagaa gctcatgatg cagggtcgga ggttcttatt gttagcaaaa
     1741 atagaagagg agatccgcat acagtgcttg cacgtggtgg tatcaacgca gctcttggaa
     1801 ccatggattc gcaagataac tggatgatac atgctgctga cactctcagt gaaggattgt
     1861 ttattgctga ctatgacaag gtagagatgc tttgcaaaag cgcacctgac gctatgactg
     1921 aacttgtaaa gtggggcgct cgatttcaca gagaacctga cggcagacta acgcagcgtt
     1981 tctttggtgc tcacacctac agaaggacat gcttctatgg tgattggaca ggaaaagaaa
     2041 taatccgtgt attgatgaaa caagttcgtc tgcgtaaaat caaatttatc gataatgtct
     2101 atattacgaa attattgaaa tcaaccaata tagaaaagac tcaaggagcg caagggaaag
     2161 gtaaagaaca agaaatctcg ggcgctatgg gcatagatct caaaagaaag aaaattatta
     2221 tttttaaagc caaatgcata atactggcaa caggaggata tactagggtc tatccagtaa
     2281 gcagttcaag aatttttgaa aactatgggg atggcgtggc tctagcgtac gaggcgggcg
     2341 tggatttggt agatatggag atggttcaat tccatccaac aggaatggtt tggccaaaga
     2401 aagctgtggg tacccttgca acagaagcaa tacggggaga agggggaatc ttgctaaatt
     2461 ctaaaggtga aaggtttatg aaaagttact atccacagag aatggagctt ggcccacgag
     2521 acgtcgtggc tcgctcaatt tataacgaaa ttatgtctgg cagaggtact aaacatggtg
     2581 gtatttggtt ggacataaca cacttgccaa aagcgaaaat tttggaaaga ttgccaagca
     2641 tgtacgacca gttcaagcga attgcaggcg ttgatatctc taaggagaaa atggaagttg
     2701 ctccaactgc ccattattcg atgggaggtg ttaaagtcga tttgaagtgc caaacaaacg
     2761 taagaggatt atttgcaatt ggcgaagtgg ctggccaagt acatggggca aaccgcttgg
     2821 gcggtaatag tctgcttgga acagtggtat ttggcaagat tgctggaagg gaagcagcta
     2881 gtaagatagc gagggaaaag aagagaacaa ccaacggcag atcttcaaca tcgtccatcc
     2941 ttggaataac atataatgaa gacaagaggc tcgcatatac tctttttgct gttaaacaac
     3001 ctcttagaat ccttcgtcag gttcaacaag tcatgcagga gaactcagga ataattagag
     3061 agggcatcaa attgcgaaaa gcaataaaga aaattttaga gttagaaaag acattttatt
     3121 ccaaacacag tgtcctaaag agctatacag attatgagaa tgttgtcaat acatgggaag
     3181 tcaagtctgc cctggttgtt tgtgaagcta tcctaagaag tgccttgatg cgacaagaga
     3241 gtaggggagc ccactacaga tctgattttc cagagagaga tgacagcaaa tggaaagtaa
     3301 atattcattg cacaaaaagt ggagaagaaa tggttatgtt caaacttcca gtgagtaagt
     3361 taagagaacc aatagaggaa ttgctaaaaa cagaaatgaa agtcgaacat catcttcttg
     3421 aataaagaat acaagatgca tcgcctcagc accgactagt ctctaacgct tagacgaatt
     3481 ttagaatatt gaagtttcca atattatttc ttatttgaat aattgacact cttgacgacc
     3541 cagaattcct gt
//
LOCUS       DASQTF010000168         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_328597,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000168 DASQTF010000000
VERSION     DASQTF010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328597"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /gene="dph2"
                     /locus_tag="VE308_03945"
     CDS             complement(<1..900)
                     /gene="dph2"
                     /locus_tag="VE308_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HZB00158.1"
                     /translation="MTIRIDEKRIFEAIRHRKPRSVALNGPEALIPKLQDVADHIAEM
                     FDIQVYIIGDTCWGSCDLNTHAADMLAADILFNIGHTIAMDTFGQKVMMIGAFDDISF
                     DVIAKKCARDLAGKYKTVSLVTDSQHLHEIDSVKKIFEDHGYNIIIGRGKGQLRDAQV
                     FGCEFYPAYNVQKQVDAYIFLGQSMFHSASVALSTEKPTFMLDPYFEEYTEVNESAQG
                     MQKKAVLSIYKALDAEKIGIVIGLKEGQFAHVRALELKKEFEKLDKKVQMIALTEITE
                     ERIQIFKGIDAFVQVACPRIGTDN"
     gene            complement(897..1196)
                     /locus_tag="VE308_03950"
     CDS             complement(897..1196)
                     /locus_tag="VE308_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZB00159.1"
                     /translation="MPIDPNFQTSSFPTTYDRLKVWKNTSSTLGIYGTDVAVDFDLCI
                     GDGGCIEVCPVNVFDWHGAGADRKAMPARERDCIYCFGCEAVCPKFAIRVTKRQA"
     gene            complement(1251..1561)
                     /gene="ffs"
                     /locus_tag="VE308_03955"
     ncRNA           complement(1251..1561)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VE308_03955"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            complement(1604..2398)
                     /locus_tag="VE308_03960"
     CDS             complement(1604..2398)
                     /locus_tag="VE308_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HZB00160.1"
                     /translation="MIEYGDKVAVAVSGGKDSLSLLYVLKLLFDQHPNNGNEIVAITI
                     DEGIKGYRDESLQIVKDFCEHLRIESKVLSYQSLFGIDMDQAMIQRSSQKISSCSMCG
                     TFRRRAIDIAAETVGANIVATAHNMDDQLQTFMINLLAGDVERIGWIYPEPVQYTQNG
                     MKKVKPFVEISEYDIAFYALQRDIPFQSEECPYMNESIRTDLREFFNRLERDHPGIKY
                     NAFRSMTKVSKVLRGGMSSSETKNCSRCGRVSTGDICSVCKTISIL"
     gene            2611..3114
                     /locus_tag="VE308_03965"
     CDS             2611..3114
                     /locus_tag="VE308_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZB00161.1"
                     /translation="MLIPSELLDEVNLRILDILSRDASRPFVDIAKELEISDATVHIR
                     VRRLMAAGILRRFTIAADNMLLGYDHLAFIGINISEGSADEVIATLSHYDEILELHEM
                     YAQFDLLLKIRARSLKEMRDIVVNKIGKIPQITEAHLMTVLKTIKEEQMISLKRDIAD
                     AASAAAT"
     gene            complement(3125..3340)
                     /locus_tag="VE308_03970"
     CDS             complement(3125..3340)
                     /locus_tag="VE308_03970"
                     /inference="COORDINATES: protein motif:HMM:NF019876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HZB00162.1"
                     /translation="MAGFCPECGGELKYDLNSKSFICKNCGLFASSEKIDELRDRAKT
                     SREDPRRKHHDEYLDWWQSSKKDKQQK"
     gene            complement(3448..3828)
                     /locus_tag="VE308_03975"
     CDS             complement(3448..3828)
                     /locus_tag="VE308_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HZB00163.1"
                     /translation="MSGTGEGKALVTIQPINFLAMVNTKTGKITDPVHELHGKSLKGS
                     VLILPYAIGSSVGAYAIYSLKEYGNAPSAVVCSKADITTASGCALANIPVVDLPNGTP
                     LSTLKQGLKTRVEADARRIIVSLS"
BASE COUNT         1009 a          937 c          847 g         1177 t
ORIGIN      
        1 gttgtcggta cctatccttg ggcaggcgac ttgcacaaaa gcatctattc ctttgaaaat
       61 ctgaatacgt tcctctgtta tctccgtcaa ggctatcatc tggaccttct tgtccaattt
      121 ctcaaactcc tttttcagtt caagtgctcg cacgtgggca aactggcctt ccttcaggcc
      181 gatgacgatt cctatctttt cggcatcaag agccttatag atggacagca ctgctttttt
      241 ttgcatgcct tgagcagatt catttacttc agtatattct tcaaaatatg ggtcaagcat
      301 gaacgtaggt ttttctgtag acaaagccac gcttgcagaa tgaaacatgc tttgtcctag
      361 gaaaatatag gcgtcaacct gcttttgcac attataggcg ggataaaatt cgcaaccaaa
      421 tacctgtgcg tcacgaagct ggcctttacc tcttccaatt atgatgttgt agccgtgatc
      481 ttcaaatatc tttttcacgc tgtctatttc gtgaaggtgt tgactgtcgg ttacaaggga
      541 gactgttttg tactttcctg caagatctct tgcgcacttc ttggctatga catcaaagct
      601 gatgtcatca aacgcgccta tcatcatgac tttctggcca aaagtatcca ttgcaatggt
      661 gtggccgata ttgaacagaa tgtctgcggc caacatgtct gcagcgtgcg tgttaaggtc
      721 gcatgaaccc cagcaagtgt cacctatgat atagacttga atgtcgaaca tttcagctat
      781 atggtctgct acatcttgca attttggaat caaggcctct ggcccattta gtgcaactga
      841 tctgggtttt ctatgcctta ttgcttcaaa aattcttttc tcgtctattc tgatcgtcat
      901 gcttgccgct ttgtcaccct tattgcaaat tttgggcaga ctgcttcgca tccaaagcag
      961 taaatgcagt cgcgctctct tgctggcata gccttcctgt cagctcctgc gccatgccaa
     1021 tcaaacacat ttacaggaca tacttcgata cacccaccat cgcctataca taaatcaaag
     1081 tcgactgcca cgtcagtgcc ataaatccca agggtacttg acgtattctt ccacacctta
     1141 agtctatcat aggttgtagg aaagctgctt gtctgaaaat tgggatcaat aggcaagata
     1201 agaaaaagga ttttgccgaa ctcaatttaa atcatgctat aaaagatagg aggcagcctg
     1261 ccggtaccac cgttggcaaa aggtcccatt tcagaccttg caagatccac cacgtagacg
     1321 tgctagcagg gttatgacgc cttagggttg caccctcccg gacctcaccc atttcggcga
     1381 tttgcggtcg agactccgcc ttcgcgtaga tcgccgctca taaccatccg gcacggcatg
     1441 atttcatttc agcaaagcaa gcgggtacct cctaccagcg gatttacccg gcagttactg
     1501 atcctggcat aaagccgatt tccagtgtgg ggtacggctg gcaccccgat cctccctgcc
     1561 tctatatacc cttggtaaaa tgactatatt tgcttgttgg atgttacaag atacttattg
     1621 tcttacacac tgagcagata tctcctgttg aaacacgccc gcatctcgaa cagttcttgg
     1681 tttcagacga agacatacca ccgcggagaa cttttgagac ttttgtcatc gacctgaagg
     1741 cattgtattt gatgcctgga tgatccctct caagcctgtt gaaaaattcg cgcaaatctg
     1801 tgcgtatgct ttcgttcatg tacggacact cctctgactg aaatgggata tcgcgctgga
     1861 gggcatagaa tgcgatgtcg tattcagata tttcgacaaa gggcttgact tttttcatgc
     1921 cgttttgagt gtattgcacg ggctctggat aaatccagcc tatgcgctca acatcgccag
     1981 ccaataagtt gatcataaat gtctgcaatt ggtcatccat gttatgtgca gtcgcaacta
     2041 tgttagctcc tactgtttct gcagctatat ctattgcacg tcggcggaat gtgccacaca
     2101 ttgaacaaga ggatattttt tgtgatgatc gctggatcat ggcctgatcc atgtctattc
     2161 caaacagtga ctggtagctg aggaccttgc tttctatacg aagatgctcg caaaagtcct
     2221 tcactatctg gagcgattca tcacgataac ccttgatgcc ttcatctatc gtgattgcaa
     2281 ctatttcatt gccattgttt gggtgttgat caaaaagcag cttcaagacg tataatagag
     2341 acaagctatc tttgccacca gaaaccgcta cggcgacctt gtcaccatat tctatcattg
     2401 aatatttcga aattgtcttt gcggccttgc tttcaattga gcacaagaag catttcttac
     2461 ataggtattc accagaatgt gctctgtagt agaggctctc tgcttttcga catttggtac
     2521 agcaaggcat atctggttgt tagccaagtc caagagttat taatcattcg agcacgaatt
     2581 gttaataaat caaaggattg tactcttttt gtgctaatac cctccgagtt gctggacgag
     2641 gtcaacctcc ggatactaga tatcttaagc cgagatgcgt cccgtccatt tgtggacata
     2701 gctaaggagc ttgaaatctc tgatgcgaca gtacacattc gggtcaggag actaatggca
     2761 gctggtatcc ttcgcagatt cacgatcgcc gcggacaaca tgctcttagg ttatgaccat
     2821 cttgctttca tagggataaa cattagcgaa ggttcggccg atgaagtgat cgccacgcta
     2881 tcgcactatg atgaaatact agagttacac gaaatgtatg cacagttcga tctgcttttg
     2941 aagatacgtg caagaagcct taaagaaatg agggatatag tggtcaacaa gatcggaaaa
     3001 atcccgcaaa ttacggaagc acatttgatg acagtactaa aaactatcaa ggaggagcag
     3061 atgatttccc taaaaaggga tattgcagat gctgcaagtg cagctgctac atagttcttg
     3121 gtatttactt ttgctgtttg tctttttttg aagattgcca ccagtcaagg tattcgtcat
     3181 gatgctttct gcgagggtct tcccttgatg ttttggccct gtcacgcaac tcatctatct
     3241 tttcagagga cgcaaagagc ccacagtttt tgcatataaa gctcttgcta ttaaggtcat
     3301 actttagctc ccctccgcat tcaggacaaa agcctgccaa ttactagccc tatttcagtt
     3361 acgccaataa aaggttttca agtacccaga ggtagcaagg gggtgcagga gctgtcgcct
     3421 attttcaggt catttgtaat agtcttgcta tgacaagctc actatgattc ttctggcatc
     3481 agcctcaacc cttgtcttca acccctgctt taatgtagac aatggcgttc cattgggcag
     3541 gtcgacaact ggaatgttgg ccagggcgca accagaggcg gtagtaatgt ctgctttgga
     3601 acacacaact gcacttggag cattcccata ttctttcagc gaatatatcg catacgcacc
     3661 gacactgctt ccaatggcat atggaagtat gagtaccgat cctttgagtg acttgccgtg
     3721 caactcatgc accgggtctg ttattttgcc agtcttagta ttgaccattg caagaaaatt
     3781 tattggctgg atagtaacta gtgcttttcc ctcgccagta ccgctcacaa tcctgcgaca
     3841 tttagaaatt atcactgggt atattcctca gcaattgtct tgatgtcttt gagagcgact
     3901 tttactctat tggacttgga gaggtagtac gctcccttga cactgtttgt aatcaccgca
     3961 tcatacttgt
//
LOCUS       DASQTF010000169         4713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_874962,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000169 DASQTF010000000
VERSION     DASQTF010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874962"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VE308_03980"
     CDS             <1..512
                     /locus_tag="VE308_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HZB00164.1"
                     /translation="EIEFELKHNGIRAYMDDRDEYTSGWKFNEWEMKGVPLRINIGPK
                     DIQAGQIEFVRRDTKEKKPAERGKLISITNSLLAQVQKSLLAKATEFLRENTFMPADF
                     ESFKSIIQGKGGFLLAGWCGQEQCESKIKGETGADIRVIPFNQKDKPATCIYCGQESK
                     KAAIYARAY"
     gene            513..1211
                     /locus_tag="VE308_03985"
     CDS             513..1211
                     /locus_tag="VE308_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZB00165.1"
                     /translation="MVALEDLFPFGTELGYIGILLISFVSSLIPFIPIPYFPVLITAS
                     FNTNFNPHIISIISALGIVGAKTIIFFISYYGRKILSDKTKKRMRPLQNLVSRYGWFG
                     AFIASLTPIPDDIVYIPLGLAKYSPWKFATATFAGKFLMNESIVWGSIFLGRPFVEDI
                     LSSDTTDMTSLIIAAIVSMIVLAVVVYFSLRIDWAKVIGRWFPWALKNGNEEEDNHND
                     NNEGNEHHTKESSR"
     gene            complement(1221..1502)
                     /locus_tag="VE308_03990"
     CDS             complement(1221..1502)
                     /locus_tag="VE308_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00166.1"
                     /translation="MLFPEHCSAKVEGEECQLAPSYIISVKSDEGEYMLAVVCNDHKS
                     ALEARLVAMQEANKIPQGSIHFQPVKAVVTDCVTGINEGYIELNDKTQQ"
     gene            1800..2081
                     /locus_tag="VE308_03995"
     CDS             1800..2081
                     /locus_tag="VE308_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00167.1"
                     /translation="MQVDKETLRLLVKRRAMLRVLSILAKQKGNALSTRILLQKLGAN
                     NLHHVILQAEKEGYIKRKRVKPQGKGNYLVYNHLTPKGRRLAELNKKLE"
     gene            2249..2428
                     /locus_tag="VE308_04000"
     CDS             2249..2428
                     /locus_tag="VE308_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00168.1"
                     /translation="MGMGSRRRKTLVRPARKSRRVDGRCPKCTTIVKFNVSGPIGDEI
                     YECTGCMSKFHISEL"
     gene            complement(2493..3347)
                     /locus_tag="VE308_04005"
     CDS             complement(2493..3347)
                     /locus_tag="VE308_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZB00169.1"
                     /translation="MLVLSFAIIAAIAVVEAGGGILSNSISLVSDAVHMFTDVMAIGL
                     SLFAITMAARSHSGAMTFGYHRAEVMVALANGVALGVISLWVLYEAFLRVMSPRIIDA
                     PLLLLTASIGLAGNLVVMFLLKHHAGNSINIHSAFIHVVYNTISSVAVIITGFIALST
                     GITIVDPLVAFLIAGLVARSAYSIVRNSTRILLEGAPSELDMREILVTLKQLDGVVDV
                     HDLHVWTISTGMDALSGHVVVRDQMLSESNKLLSEINKVLAERYNITHTTIQMEHERE
                     ISFRRTIR"
     gene            complement(3406..>4713)
                     /locus_tag="VE308_04010"
     CDS             complement(3406..>4713)
                     /locus_tag="VE308_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZB00170.1"
                     /translation="LDNEVLQSEADLLSTELEKYSRRLTLLSSAMEKLSSRLQSVREE
                     PSSKKELADKNIAHSQILKAIDVANIELGQTISILGGLESKLELDMQHLADMNQENER
                     LSLELQERKRQKEEMNRKSQSLEAELKGIRDQEQQMIASSGSAYSILQEYERKIKALS
                     ESERKMSKEYNLIERESALLRKDIADLTGEESRQINNLLSLGYKNLLDEMDVEIAIKE
                     LTDEYEEVKSRINLRADEAYVQVMDGYRSMSTRRNQLESERNSIVSFIEQIVKEKEKV
                     FMEAFQKVDSDIRATFEKMTGGLAWLEIQNSEDIFSSGVMLLVRFPGKTVPRESTTLS
                     GGEKTIAATVFLLALQSLKPSPFYLMDEVDAHLDAENTERLSKVLLERSKDSQIIMVT
                     LKDSTVAKAALIYGIYPKEGASQVVKYKNPARLALAPVSGNNL"
BASE COUNT         1331 a         1047 c         1039 g         1296 t
ORIGIN      
        1 gggaaataga atttgaatta aagcacaatg gtataagagc atacatggat gaccgagatg
       61 aatacacttc tggttggaag tttaacgaat gggagatgaa gggagtcccg ctgcgcatca
      121 acattggacc aaaggatatc caagcggggc agattgaatt tgtcaggcgc gatacaaagg
      181 aaaaaaagcc tgctgaacgg gggaagctta taagtatcac aaactcactt ttggcacagg
      241 tacagaagag tctgcttgca aaggcaaccg aatttctcag ggagaacaca ttcatgcctg
      301 ccgattttga gagcttcaag tcgataatcc aaggcaaggg cgggttcctt ctcgcaggct
      361 ggtgtggcca agaacaatgc gaatccaaga taaagggaga gacaggtgca gacattcggg
      421 tgataccatt taaccagaag gacaagccgg ccacctgcat ttactgtggt caggaaagca
      481 aaaaggcagc gatatatgca agggcatact aattggtagc gctcgaagat ctctttccat
      541 ttggaactga actaggatac ataggaatcc tcctcatcag ctttgtgtct agcttaattc
      601 catttattcc cattccttat tttccggtct taataactgc ttcatttaat accaatttca
      661 atccgcatat aatctccatt atcagtgcgc ttggcatagt gggagcaaag acgataatct
      721 tttttattag ctattatggt cgcaaaattc tgagcgacaa gacaaagaag agaatgcgtc
      781 cactacaaaa cctagtaagc aggtacggtt ggtttggggc atttatagca tctcttactc
      841 caatacctga tgatattgtc tatattccgc ttgggcttgc caagtacagc ccatggaaat
      901 ttgcaacagc cacatttgct ggcaaattcc tgatgaatga atcgattgtg tggggctcta
      961 tctttcttgg gagacctttc gtcgaggaca tattatcatc agatacgaca gacatgacat
     1021 cattaatcat agcagcaata gtgagcatga tagtgcttgc agtagttgta tacttttcgc
     1081 tcaggataga ctgggcaaag gtaattggaa gatggtttcc ttgggcgctg aaaaatggta
     1141 atgaagaaga agataatcat aatgacaaca atgaaggcaa tgagcaccac acaaaagaga
     1201 gctctagata ggtttcgata ctactgttgt gtcttgtcat tgagctcgat ataaccttca
     1261 ttgatgccag ttacgcagtc agtgaccact gccttaactg gctgaaaatg tatactgccc
     1321 tgtggaatct tgtttgcctc ttgcattgca accagccgcg cttcaagtgc acttttgtgg
     1381 tcattacata ctactgcaag catgtattcg ccctcatccg attttactga gatgatatat
     1441 gagggagcca gctggcattc ctctccttca accttggctg agcaatgctc ggggaaaagc
     1501 atatcatctt tcttaatctc aaggcaatat aacaataact gacctgtaaa ctatccaaag
     1561 atataaaatt caagaaatga tgccagtcat gcattttttt cctgtaaatg aaaatccagc
     1621 ctaggaacta cagtagagta gcaacattag tggacccgac actagcacga aaacgtgcat
     1681 tagtgagata cttataatga ctatggcagg agtttacaag ctttagctgg ctcgtgggat
     1741 tcaaatataa ttgatgtaac tattatatat tcctatttaa taggaatata ttgacgctat
     1801 tgcaagtcga taaggaaaca ttacggcttt tggtcaagcg aagagcaatg ctccgagtgc
     1861 tgtctatcct agccaaacaa aaggggaatg cgctgtctac tcgtatactg ctccagaagt
     1921 taggagctaa caaccttcac catgtaatcc tgcaggcaga aaaggaagga tatatcaaac
     1981 gcaaaagagt taaaccacaa ggaaagggca actatctggt ctacaaccac ttgactccaa
     2041 aaggaaggag gttagctgag ctgaacaaga aattggagta aaagtaatga taacttacct
     2101 aaaactagac ctcatatttt ctgttataac atgatagggt tggaacagac tgcatcattg
     2161 gcggaacgat acgactatag acatgagaag taaagaaagt tatgcaatta gtacatctta
     2221 atttataaaa cggcagaggg ttttttgtgt gggtatgggt agcagaagaa gaaaaactct
     2281 tgtaagacct gcaagaaagt ctcgcagagt ggatggaaga tgtcccaaat gcaccaccat
     2341 agtgaagttc aatgtgtctg gcccaattgg cgacgagatt tatgagtgca cgggatgtat
     2401 gtccaagttc cacataagcg agttgtaaaa acctaatagc gctaaactgc ttgggaaaag
     2461 cccgaccagc caaagcaaaa aattattgct actcatctta ttgtacgcct gaatgatatt
     2521 tcgcgctcgt gctccatctg tatagtagta tgggttatat tgtagcgctc agccaacact
     2581 ttgtttattt cagagaggag cttgtttgac tcgcttagca tctggtcgcg tacgaccaca
     2641 tggccactaa gtgcgtccat gccagtcgat attgtccaga catgcagatc atggacatct
     2701 accactccat ccagctgctt aagagttact aatatttctc gcatgtcaag ctccgacgga
     2761 gcgccctcca gcagtatacg ggtgctattt ctcactatag aataagcact tcttgccact
     2821 aggcctgcta tcagaaaggc gacaagcgga tccacaatgg tgatgccagt gctaagagca
     2881 ataaagccag taatgatgac tgcgacagat gatattgtgt tatagacaac gtggatgaat
     2941 gcactgtgaa tattgatact gttgccggca tggtgcttta gcaggaacat caccactagg
     3001 ttgcctgcaa gaccgatact tgctgtcagt agtagcagcg gcgcgtctat tatccgcgga
     3061 gacatgacgc ggagaaaagc ttcgtacaaa acccataacg aaataactcc aagcgctaca
     3121 ccgtttgcaa gtgcaaccat cacttcagct ctatgatagc caaatgtcat tgccccagaa
     3181 tggctccttg ccgccattgt tattgcaaaa agacttagtc caatcgccat cacgtcagtg
     3241 aacatatgca ctgcatcgct cacaagcgaa atgctgttac ttaaaattcc acctccagct
     3301 tcaacgaccg ctattgcggc gatgattgca aatgacaaca ccaacatcct cttgggccgc
     3361 agcaagctag ttcccaatgt agatagtcta tgctgttttc ggtatttaca gattgtttcc
     3421 gcttactggg gcaagcgcta accgcgcagg attcttgtac tttacaacct gggaagctcc
     3481 ttcttttggg taaattccat agataagggc agctttggcc acagtagagt ccttcaaggt
     3541 gaccatgatt atctggctat cctttgagcg ctcgagcagt acctttgaca gtctctcagt
     3601 gttctcagca tcaagatgcg cgtctacttc gtccatcaaa taaaatggcg acggtttgag
     3661 tgactggaga gcaagtagga acacagtggc agcaatcgtc ttttcgccac cagacagtgt
     3721 cgttgattca cgggggacgg tcttaccggg gaaccgcact agcagcatta caccgcttga
     3781 aaaaatatct tcagagttct gaatctcgag ccacgccaag cctcctgtca ttttctcaaa
     3841 tgttgcgcgg atatcgctgt caactttctg aaacgcctcc atgaagacct tttctttttc
     3901 tttcactatc tgctcaatga atgaaacgat agaattacgc tcactttcaa gctggttgcg
     3961 cctagttgac atgctacggt agccatccat tacctgtaca tacgcctcgt ctgcgcggag
     4021 gttgatcctt gacttgactt cctcatattc gtcagttaac tctttgatcg ctatttcgac
     4081 atccatctca tcaaggaggt tcttgtaacc aagagacaaa agattgttta tctggcgaga
     4141 ttcctcacca gtcaggtctg caatgtcttt tcgaagcaac gcagactccc gctctattaa
     4201 gttgtactcc tttgacattt tgcgctcgct ctctgagagt gcttttatct tgcgctcata
     4261 ttcttgcaat atgctatatg cgctgcctga agaagcgatc atctgctgtt cctgatccct
     4321 gatccctttg agctccgctt caagtgactg cgacttccta ttcatctctt ctttttggcg
     4381 tttgcgttcc tgtagttcaa gggaaaggcg ctcattctct tggttcatgt cggctagatg
     4441 ttgcatatca agttccagct tggattcaag tccgccaagt atcgatattg tctggccaag
     4501 ctcgatattc gctacgtcta ttgcctttag tatctggctg tgtgctatgt tcttgtcagc
     4561 aagctccttt ttacttgacg gttcttccct tacgctctgt agccttgacg atagcttttc
     4621 catggcagaa gaaagaaggg taagcctcct tgaatatttc tcaagctctg tcgaaagcaa
     4681 gtcggcttct gactggagaa cctcattatc taa
//
LOCUS       DASQTF010000170         4545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1072861,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000170 DASQTF010000000
VERSION     DASQTF010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072861"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..620)
                     /locus_tag="VE308_04015"
     CDS             complement(360..620)
                     /locus_tag="VE308_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00171.1"
                     /translation="MHRNVAVKYLMKRRRSLLMTKNSEKVEKLWEDLPKEIKIIGKQV
                     TKEYRIRWERLGSEEFEGSRFAFTLEDQGERSSNNGPFSKDS"
     gene            complement(1439..2530)
                     /locus_tag="VE308_04020"
     CDS             complement(1439..2530)
                     /locus_tag="VE308_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00172.1"
                     /translation="MQANEVQQVIILSTYKSLIMTYKMHILSVPALVLVVILSFGTSA
                     YSSDDLVKIIAQSSFVDSEGKMNIVGTVRNTGSLPVQTMVGLEVQDETGIRIERQLTY
                     GRIIWPLNDSPFKFVIDSGTAGHPFIADVKEVDPTHITDMIILNYTSMAAGEERAFVG
                     TVKNNAPFDIYNVSVFASARSKNATQLDSVRSDVIPVLKASEEQPFIAITDAAVKSRV
                     YYYSCAGLDLDAPITTIDAGGGRFIAYDLNAAAQVSKIRYENETDSIAFGVRPYSPTG
                     GPISLMIPQASQEQSVTVMLDGKLHESSIRDDGKTIYIDFFVPQGEHQVQIQRVTSMP
                     EMPFAMLGLAVVTAAVLAAARLRTAFKIH"
     gene            complement(2625..2792)
                     /locus_tag="VE308_04025"
     CDS             complement(2625..2792)
                     /locus_tag="VE308_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00173.1"
                     /translation="MKMLNIDEQTYRRLASIMNDMMHTEKRNISYDDAINELIDVYQD
                     RLALSGESAGG"
     gene            3180..3710
                     /locus_tag="VE308_04030"
     CDS             3180..3710
                     /locus_tag="VE308_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HZB00174.1"
                     /translation="MPYTPKDAAILLQRARELVEPEASIRDARISKKYIEFDTSIPDG
                     TDVKKIMTRLEAIAPLASHEQILERHMDKDIAIKRAVQLFNDERYWEAHEALEYVWKN
                     ATGIEKEILNGIILIAAAFVHDEKDEQDVCISILQRARKKLDRDSGIYQGIDTNRIAD
                     MVSEIIKTGIVERFTI"
     gene            3856..>4545
                     /gene="prs"
                     /locus_tag="VE308_04035"
     CDS             3856..>4545
                     /gene="prs"
                     /locus_tag="VE308_04035"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HZB00175.1"
                     /translation="MFYLSETLTEISVVAGPGSFDLAKNIAKRLDAQLTVASLRIFSD
                     GESNIKLGKVGKNCVIVQSTHPPTDTRLIQLLMMAKKCTDDGAQHVCAVIPYLGYARQ
                     DRAFLEGEVVSIGLVAKLFETAGLKHIITVDIHSQLAMSYFESIQNISSVPLLADYAS
                     KIKLRDPIAVSPDAGGTNRAKEFARHLNIDVIVLKKSRHRVTGEVTVDEKLHMDISKR
                     DAIVIDDIISSG"
BASE COUNT         1301 a          967 c          996 g         1281 t
ORIGIN      
        1 gtgatttttc tgaaagactc gggattaacc agctgtgact aaattgtaga aacgatgtat
       61 tgaatctgat agaaaaaacg tacccatctc tagaaccgaa gataaggcag agatatgaaa
      121 tatgatcgta agtcttatcc acgtcaaaga aagaggattt gatgacaacg gaagattcag
      181 aatcaacaag ataatgtaca aaggttctct tcatgcactc agctaaacca acatatttca
      241 gattttcaaa ttctaaggaa gaaagggctt gtgactacga gatcatattc aactttcaac
      301 atgttttaaa aagtctccct ctttacaaag aggcaagaca ccaggctaag ctggataatt
      361 taagagtcct tagaaaaagg accattatta gaagatcgtt caccctgatc ctctaatgtg
      421 aatgcaaaac gtgagccttc aaattcttcg cttccaagcc tctcccacct tatcctatat
      481 tccttggtga cctgcttacc tattattttg atctcttttg gtaggtcttc ccatagcttt
      541 tctacttttt cagagttctt cgtcatcaaa agcgacctgc gccttttcat aagatattta
      601 actgccacgt ttctatgcat ttggccttca acaggtattt gttcttttcc tgtatctatt
      661 gtaaactgac gtttagccaa tgcaataatc gtaagattgt atatagaaaa gctttgtggc
      721 taaaaagaat ctagtgatga ggatgattct atgccatgct gctatttttg cccatctggt
      781 tgatcgaatt tagccattct tccatatgca gagttgtcaa gaccacaaca tcatttaagg
      841 attgaattag ttttctatat gaacatgagg gtgcgcctta tatcaattct gtgacgctta
      901 tggtctctcg atagtaccat agcataatga tcacaatatc tttaactgag atacactaat
      961 aggcaattgt ttgccctact taggatctaa gggaaagcca aggtctgcac gccgagcgcg
     1021 aaaaccactg caggacaatt ggcccaagac agctgtgggt aagtggctaa gatgcaccta
     1081 cagaagatgt ggatttgaat ggcagtactt tgggggtagg tattgggctg aatgtccggc
     1141 ttgtcactcc acaatgaagg ttgcaatggc gcggagaaac tatagactag ctgacaaaga
     1201 ataaattttc gtcgtgcgct aacgacaggt ctacggtatt tctatcctta tcatgtataa
     1261 ccagcaggct ctttttgatc ctatgctttc ttgctagagc ttgcatatgg aaattcagcg
     1321 tataagaatc gttaaaagag tctgattcta ctaatgcaag gctttcgctg tagacactta
     1381 gcttcaaacc tcccgaagat ttctctatta aagtgacata cacgagatgt ttgagtggtt
     1441 agtggatttt aaatgctgtc ctgagtcttg cagcagccaa cacggcagct gttaccactg
     1501 ccaaccctag cattgcaaat ggcatctctg gcatacttgt gactctttgg atctgcacct
     1561 ggtgctcacc ttgcggaaca aaaaagtcga tatagattgt cttgccgtca tctcttattg
     1621 aggattcatg caacttgcca tctaacatca ctgtcacact ctgttcctgc gatgcttgtg
     1681 gtatcatcag gcttatgggt ccacctgtcg gactataagg ccttactccg aaggctatgc
     1741 tatctgtttc attttcatac ctgattttgc ttacctgagc ggctgcattt aaatcataag
     1801 caataaaccg gccgccaccc gcgtctatcg tagttatggg ggcgtcaagg tcaaggcctg
     1861 cgcagctgta gtagtatact cttgatttga ctgctgcatc agtaatggca ataaatggtt
     1921 gctcctcgct tgccttcaga acagggatca catcgcttct tacactgtca agctgcgtcg
     1981 cattcttgct tcgcgccgag gcaaagacgc tgacattgta aatgtcaaaa ggagcattgt
     2041 tcttgactgt accaacaaaa gccctttctt ctcctgccgc catgctagta tagttaagta
     2101 tgatcatatc cgtgatgtgt gttggatcaa cttccttgac atccgctata aatgggtggc
     2161 ctgcggttcc agagtcgatt acaaacttaa aaggcgagtc gttcagtggc cagatgatac
     2221 ggccgtatgt cagctgacgc tcgatcctaa tcccggtttc atcctgcacc tctaacccca
     2281 ccatggtctg aactggtaag ctgccggtgt tccttactgt gcctacgatg ttcattttac
     2341 cctcagaatc aacaaatgat gattgggcaa ttatttttac caagtcatcg gaggaatagg
     2401 cacttgtacc aaatgataaa atgactacaa gcactagtgc aggcacagat aatatgtgca
     2461 tcttgtaggt catgataagt gatttgtagg ttgataagat tatgacctgt tgaacttcat
     2521 tggcttgcac cgcaaggtgg aaatttctgc atttaggaga tttcgataag gccggcatga
     2581 tttcagctgg cagccatatg cgcgccaccg caatttaagt ttccctaacc cccggcactt
     2641 tctccgctta acgccagacg atcctggtac acatctatta attcatttat tgcatcatcg
     2701 tagctaatgt tgcgcttttc tgtatgcatc atgtcattca taatcgatgc taacctccgg
     2761 tatgtctgct cgtcaatatt cagcatcttc attattttgg tgcaaaacct atgaggatat
     2821 tttattgttt caaatcagca gcgcgtggca aggcacttat cagcatactg ttgaaagcag
     2881 tgaaagattg gaacctgcac ttcttccggc ggcggcggca tgaatgctct agcggctatt
     2941 gtcttgccta gaaggaagaa aaggcaatgg agatgcctac aaggctagct gctcttgcag
     3001 atattaacaa tagttttgct ccaagtaaat accatacatt tacaggcaga ttagtccatg
     3061 tgaccagtgt taatatggac tatattccta gtcatgctat ttcaagacag gtttgttttt
     3121 gtagcaggat aatgcaatat cagtgcatga ggcgatacat gttgcatcta cagaatactg
     3181 tgccatatac tccgaaggac gctgctatcc ttcttcaaag ggcaagggaa cttgtagagc
     3241 ctgaagctag catccgcgat gcccgtattt ccaaaaaata tatcgagttc gatactagta
     3301 tacctgacgg tactgatgtg aaaaaaataa tgacaagact tgaagccatt gcgccattgg
     3361 ccagccatga gcaaatccta gagcgccaca tggataagga catagccatc aagcgtgcag
     3421 tgcagctatt caatgatgaa aggtactggg aagctcatga agcacttgaa tatgtatgga
     3481 aaaatgcaac agggattgaa aaggaaatcc taaacggaat catactaatt gcggctgcat
     3541 ttgtacacga cgaaaaggac gagcaggatg tctgcatctc tatactacag agggctagaa
     3601 aaaaacttga ccgagacagc ggtatatacc agggaattga cacaaacaga atcgcggaca
     3661 tggtttccga aattatcaag acaggaatag tagagaggtt tacaatatga aagctcagag
     3721 tatagacatt atggctgctc atggtgtcgg agcataatca tgaaagtgcc tagcagacct
     3781 cagaaaaacc gtctattgaa gattggcaga ttaattgaag agcctgttgt cacacagaat
     3841 agatattagg atgctgtgtt ctacctgtct gaaacattga ctgagatatc agtagtagcg
     3901 ggaccgggat catttgatct tgccaagaat attgcaaaga ggcttgacgc ccagctgact
     3961 gtggcaagtc tgagaatatt ctcagacggc gaaagtaaca tcaaactcgg caaagttggc
     4021 aagaattgtg ttatcgtgca atcaacccat ccacctactg atacacgcct gatacaacta
     4081 ctgatgatgg caaaaaagtg cacagacgac ggggcgcaac atgtctgtgc ggttattccc
     4141 tatcttggtt acgctcgcca ggacagggct tttctggaag gcgaggttgt gagtattggc
     4201 ttggtggcga agttatttga aacagcaggg cttaagcata tcatcactgt tgacatacac
     4261 agtcagcttg ccatgtcata tttcgagagc attcagaata tttcttcagt tccactccta
     4321 gcagactatg cctccaagat aaaattacgt gatccgattg cagtgtcccc cgatgcaggc
     4381 ggaaccaata gggctaagga gtttgcccgg catctcaata tagacgtcat tgttttgaaa
     4441 aagtctcgtc atagagtcac tggtgaggtg actgtagacg aaaagcttca catggatatc
     4501 tctaagagag acgcaatagt gatagacgat ataataagca gcggc
//
LOCUS       DASQTF010000171         6962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_797513,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000171 DASQTF010000000
VERSION     DASQTF010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797513"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1074)
                     /locus_tag="VE308_04040"
     CDS             complement(133..1074)
                     /locus_tag="VE308_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZB00176.1"
                     /translation="MDVNDNINGASTNQQGYKIDPAMKMGHVSLNVSNLSQSLDFYQS
                     VLGFKAVGRPSSGKALLSVGSNDPSYLVELLQVKTGSNNDSLSRLESSTRRAGLYHFA
                     ILLPERKFLADMLQNLNYMRDQVHFDGLADHLVSESIYIRDPDFNGVEIYSDRPRSQW
                     RWKDTKVEMATLPLNTTDLLKEATGKAWKEMPDKTTIGHVHLHVGDLSKAIKFYYEIL
                     GLQLTAAVPSALFFAVGGYHHHIATNTWLGTGIAPASSESIGLNHFSIELPSKEELAT
                     LVKVFSRRSMAVIEGNLSESSVFIRDMDSVKIQVEVK"
     gene            complement(1817..2107)
                     /locus_tag="VE308_04045"
     CDS             complement(1817..2107)
                     /locus_tag="VE308_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HZB00177.1"
                     /translation="MTSSKKELKEKENQQRLARAIETLDSITRNSDIQKSIRNMIKEV
                     LLALRDEKSGSLSVRAANAISLLDSATQSPQMQSHIRTMLWQVVSTLESIRE"
     gene            2570..2767
                     /locus_tag="VE308_04050"
     CDS             2570..2767
                     /locus_tag="VE308_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00178.1"
                     /translation="MVAEEQIKCNICGGITVNVSQVKQHASTPSHELNKSKLEQKLNA
                     VRIENYQNDSSVIMSWNKSST"
     gene            3045..4223
                     /locus_tag="VE308_04055"
     CDS             3045..4223
                     /locus_tag="VE308_04055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZB00179.1"
                     /translation="MIANIVHISLNARGGGERLAVATIKAISSMGIGVELSTVEKPDM
                     QLIHDAYGTTIDGDLKKIRTLNILQKYSPHNYSLTVNTHGDMLPFYHKDFTKRNAITY
                     CHYPIARYLIDSCDPDYSAVLQNMCLLGMTPAVRSTYYEAARSAYIKMMLNSTVLTNS
                     EFSRKAIFKTFAVDSTILPPPVNVDIFRNMCLASNVRDDCILVISRFHPSKKIENAIH
                     LAKLLHKNEVGTCMNIVGNMPPDGIGYFNYLNDLVRHYDLEDFIRFEINVRFDRLLDL
                     MRRSKVYVHPLPGEPFGMSTVEGMSAGIIPVVPDIGGHTEFVPEKYQFHTYGQGVQAV
                     AAALAAPASERIKLSHSTQKYSVTNYVKKFQQILVEVMDIGKKHVESKPVISLSKRLS
                     "
     gene            complement(4240..5088)
                     /locus_tag="VE308_04060"
     CDS             complement(4240..5088)
                     /locus_tag="VE308_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00180.1"
                     /translation="MREKEMTIDDSLAEAVILLKNQFVQDYKNRMGLLTEIIPRYYSK
                     SLPISNDLLDYLHRFATANPIYFKSHDAAISGIPCRIYEGDINNYWLSSKKHDTSYQP
                     FYPTWMLSAFTLAQESKSLGFDQLVDVGSGDGRLAYCASLLGIESYGIEIDDELVELQ
                     DAVSYNTGIKYNAIRADATRFDYGSLHLSKPIFFISGLPEIGEMLANSVIRQVLSVEK
                     MKYNAGFNFMGSHVMKSLTRDRTGWGWGNIIANFGLKFIGTTTLPTLWTTDQIQDTAY
                     VYTRAR"
     gene            5536..5748
                     /locus_tag="VE308_04065"
     CDS             5536..5748
                     /locus_tag="VE308_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00181.1"
                     /translation="MNCHDFIVMWSSVVLFVLTFAVLGLLLGFAAVYDTNQEGPMGEA
                     LIHENVTANATQGNQSGSPNTNEESE"
     gene            complement(5782..6711)
                     /gene="sucD"
                     /locus_tag="VE308_04070"
     CDS             complement(5782..6711)
                     /gene="sucD"
                     /locus_tag="VE308_04070"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZB00182.1"
                     /translation="MTTTVEKTFNIFDTLIGTEGDEDFGRKPVIIQGMTGSYGSTHTR
                     LMKAYGTNIPAGVTPGKGGQRFEGTPVYNTMSEAVKATSAQISGIFVPAPFFLKAAIE
                     AIDSGIKLLVAIPEHIPIRDAIKVLEYARKNGAKMIGPNTPGVIVPEVMKVGIMPAQP
                     FKGGATVVFSRSGTLMYDVSYNLSSKGFGQRLCLGIGGDPINGTNLIEAFDLVRDRDD
                     VDSIVVVGEIGGDAEEQLAQYIIRTDFIKPVIAYIAGRAAPKEKRMGHAGAIVYGNYG
                     SAESKVANYSRANVPVAKRPAEVPELLAKKLIK"
     gene            complement(6708..6935)
                     /locus_tag="VE308_04075"
     CDS             complement(6708..6935)
                     /locus_tag="VE308_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00183.1"
                     /translation="MNLFGGIVRTNLVAQAILDAYNNNLMNVPVFARISGADSEKARE
                     MLQGSKAKLYNTVEEAIQSAVSALNSSRGSK"
BASE COUNT         1924 a         1551 c         1383 g         2104 t
ORIGIN      
        1 atgtcattac ttgcatcctt tctcttccct ttcgagttca ctgatcttag gaggtgattt
       61 tgcatttgta tttaattcaa taatcattcg agtaattttt cgttcaaagt cgtcttatgg
      121 ttcacaagtg catcatttga cttcaacttg tatttttacg ctatccatat cccgtatgaa
      181 cactgagctc tctgataaat tgccctctat tacagccata ctgcggcgtg agaatacctt
      241 aaccaacgtt gcaagctctt ctttgcttgg aagctcaatg ctaaagtggt tcaagccaat
      301 gctttctgat gatgctgggg caatgccagt tccaagccag gtattcgttg ctatatgatg
      361 atgatatcca ccaacagcaa agaacaaagc acttggaaca gcagctgtca attgcagtcc
      421 tagaatttca tagtagaact ttattgcttt tgacaaatct ccgacatgca agtggacatg
      481 accaattgtg gtcttatcag gcatttcttt ccaagccttt ccagtagcct ctttcaaaag
      541 gtcagtagtg ttcaatggca atgttgccat ttcaacctta gtgtccttcc atctccattg
      601 agaccttggc ctgtcactat aaatctcgac gccattgaag tctggatccc gtatgtagat
      661 ggactccgaa actagatggt ctgcaagacc atcaaaatga acttggtcgc gcatgtaatt
      721 tagattctgg agcatatccg ccagaaactt tctttcagga agcaagattg caaagtggta
      781 gagtccagct cttcgagtag aactctccaa ccgactaaga ctgtcattgt ttgaacctgt
      841 ctttacttgc agcaattcaa caagatagga agggtcgttg ctgccaacgg atagcagtgc
      901 tttaccgctt gaaggtctgc cgactgcctt aaatcccaaa acagattggt aaaagtcaag
      961 tgattggctt agattagaca cattcaggct tacatgcccc attttcatag ctgggtctat
     1021 tttgtatccc tgttgatttg tggaagcacc attaatgtta tcgttcacat ccatgatgat
     1081 agataggcag tatattgcac tatatcacta atactcgcgg tagttattgg ttctatcaaa
     1141 tctccgggtt atccaagaat ggtgatgaga tgattattat atcataatta ggaaaaagcc
     1201 tgctgccggt aaagatgata gttaccaatc gttattcgac taatatacac acgttcatac
     1261 gacatatcat ctttgtatct acagtattgg atgtgatgga tggtagaaag gaatatcatg
     1321 atcatgatac tagccatcat atgcagttat cgaccacctg gcagatagtc aggagtatca
     1381 cgatgccaaa agaaagatga tcgagaaaat atagaagtgc atatatttgt aaaccacttt
     1441 caaagagtat tatttcgaaa gaaaatggca agactatgca agttatgact ttgaatcctc
     1501 taaaatcgtt aattatatga aaacagataa tcctatatag agatctctca agagaagata
     1561 taacgttaga tttcacaatg ttaaacccat cgacattgtg cgctagcgtt aattttctat
     1621 attgttattt ccatactgtt tttagccatg taggttcaat accttttgca cttacaatat
     1681 ggctatacga tatagatgtg tccactgcaa tgttgtctta agcggggaag gcgcttcgag
     1741 gcattatctt gccaatccta cccacaagct cgagaagcta cctcctccag taaagccggt
     1801 gaagaacact cgttagctat tctcttatac tttctagagt cgagactact tgccacaaca
     1861 tggtgcggat gtgtgattgc atctgagggc tttgagtcgc actgtctagc aaacttattg
     1921 catttgcagc cctcactgaa aggctcccgc tcttctcatc cctgagtgca agaagcactt
     1981 cttttatcat gtttcggata ctcttctgga tatcactgtt tcttgtaatt gagtccagag
     2041 tttcaatggc gcgagcaagg cgctgttgat tttctttttc tttaagttcc ttttttgatg
     2101 atgtcattat acaggcctcc aaacgactgc ttatctacct gctagttgct tttgaatgtt
     2161 tttgtggtcg ttggttctca aagacactcg cctattccgt attgaatcat gtatatatac
     2221 tccctatgga tccgcttcta aatcaagaaa aatattcgac gttagcctct tctactgact
     2281 gcccaaagca gcatcctttt aagagattct gcatgatgtt atattcgcgc ctatcctcac
     2341 tttgacgagg ccagcccaag aggggaggag aagagaagct cttcaggttc gctgcagaag
     2401 aaataataat tcttgtttag caatttttag aaggtggtcc tatgtataat ggatatgcag
     2461 ttttatctgg atcgatcgcc atagatatat atattttata atactgtatc tattagatgt
     2521 tttatggctt attgtttatg tattttcaga ggttgatcaa tccaagtaaa tggttgcaga
     2581 agagcaaata aagtgcaaca tttgtggcgg catcaccgtc aatgtaagcc aggtaaagca
     2641 acacgcatca acaccttctc atgagttgaa caagtcaaag ctcgagcaaa agctcaatgc
     2701 tgtaaggata gaaaattatc aaaatgatag ctcggtaatt atgagttgga acaaaagctc
     2761 tacgtaatta tgtcatttca agccctcaag aagcacttgg catcatagag cagcagggag
     2821 gtaccgagct tgcaatcatg atccttcaaa ccatcaaata accatccaaa ggttttgtcc
     2881 caagctagtg atctctcgct ctcaatagta tctcttatac ataatagctt gcgagctatg
     2941 cattcaattt tatcgaagtt tcttattagg aaatcttttt cgatcatctg taggaaggga
     3001 attaaatagc agttaatttg cagtattgtg tatatcacat tggtttgatt gcaaatattg
     3061 ttcatattag tctaaatgct cgaggaggcg gcgagcgtct tgcagtcgcc acgataaagg
     3121 cgatttcatc tatgggtatc ggcgtcgagc tctccacagt ggaaaagcct gatatgcaat
     3181 tgatacacga tgcctatggc acaaccatag atggggatct caaaaagata agaacgctca
     3241 atatcctcca aaagtatagc ccccacaatt atagccttac ggttaatacg catggggata
     3301 tgctcccatt ttaccacaag gatttcacca agaggaatgc gatcacatat tgccactacc
     3361 ctattgcaag gtacttgatt gattcctgtg accccgatta ctctgccgtt ctacagaata
     3421 tgtgtctttt aggtatgaca cctgcagtcc gcagtacata ctacgaggcg gccaggagcg
     3481 cttacatcaa aatgatgtta aactctaccg tgcttactaa ctcagaattt agccgcaaag
     3541 cgatctttaa gacctttgct gtggattcga ccatcctacc cccgcctgtc aacgtagata
     3601 tattccgcaa catgtgtctg gcatcaaacg tccgtgatga ctgtatcttg gtgatctcgc
     3661 gcttccaccc tagtaaaaag atagaaaacg ccatacatct tgccaagctg ctacataaaa
     3721 acgaggtagg cacatgcatg aatattgtag gaaatatgcc accagacggc attggatatt
     3781 tcaattatct gaatgatctt gtaaggcact atgacttgga ggattttatc agattcgaga
     3841 taaacgttag atttgatagg ctgctcgatc tcatgcgcag gtcaaaggtc tacgtacacc
     3901 cgctacccgg agaacccttt ggcatgtcta ctgtggaagg gatgagcgca ggcataattc
     3961 cggtagttcc agatatagga gggcatacag aattcgtccc tgaaaaatac caattccata
     4021 cctacgggca aggagtacaa gccgtggcag cagcacttgc tgcacctgca tcagaacgga
     4081 tcaagttaag ccactcgaca caaaagtact ctgttacaaa ctatgtcaag aaattccaac
     4141 aaatactggt cgaagttatg gatatcggca aaaagcatgt ggaatcaaaa cccgtcattt
     4201 cactttcgaa gaggttgtca taatccatgg ctgcctgacc tagcgagctc gcgtgtacac
     4261 atatgcagtg tcttgaatct ggtcagtagt ccagagtgta ggcaaggtgg tggtccctat
     4321 aaatttcaga ccaaagtttg caatgatatt tccccatccc cagccggtcc gatctcttgt
     4381 gagcgacttc atgacatggc ttcccatgaa gttaaagcca gcattgtact tcatcttctc
     4441 aactgacagc acctgtcgga ttacgctgtt tgcaagcatt tctccaattt ccggcaaacc
     4501 cgaaataaag aatatcggct ttgacagatg caagctgcca taatcaaagc gcgttgcatc
     4561 tgcccttatc gcgttatatt tgatccctgt gttatacgac actgcatctt gcagttcaac
     4621 aagctcgtcg tcgatctcta tgccatatga ttcaattcct agaagacttg cgcaatacgc
     4681 taacctccca tcgccagaac cgacatctac cagctggtca aacccaagcg actttgattc
     4741 ttgcgcaagc gtaaaagcag acagcatcca cgtagggtag aatggttggt agcttgtgtc
     4801 atgtttctta ctgctcaacc aataattatt gatgtcgcct tcataaatcc tgcatggtat
     4861 tccagaaatc gctgcatcat gtgacttgaa gtatatagga ttggctgttg caaacctgtg
     4921 taaatagtca agtaaatcat tactaatggg caaagactta gagtagtatc gaggtatgat
     4981 ttcagttaac aggcccatcc tatttttgta gtcttgaaca aattggttct tcaggaggat
     5041 tacggcttct gcgagggaat catcaattgt catttctttt tctctcaata gactgattcc
     5101 gcacttggtc gcaaacctct tctcatgctt ttatgaccac agtctttaca acgatagagc
     5161 cttctgcgct tgtaagtgta gatggtttcc ggatcctcgg ctatttcatg acgtgtcttt
     5221 gccatgcagc ggtcacaaaa tcgaagctct gttgacaaaa tatgtgtcta tgggataaac
     5281 atctgcactt tcgaatatat attatatgga tcattactat tatttgaaaa gaagtaaact
     5341 cgaagctact tgctatatct tgtgttacat ccttttactc aattttacag tggaaaatag
     5401 tccttgatgt taattataca aatagcaatt tctttctcct ccaacaagaa attaggacaa
     5461 gaacacaatt catattctca cattgcggct ttccatttca cgcaatctct tacaaaagta
     5521 aatttttata actcaatgaa ctgccatgac tttatagtca tgtggtcaag tgtagtgtta
     5581 tttgtactca catttgctgt ccttggactt ttgctgggat ttgcagcagt ttacgataca
     5641 aaccaggagg ggccgatggg cgaagccttg atacatgaaa acgtaactgc aaatgcgaca
     5701 caaggcaatc agtctggcag ccctaatact aatgaagaaa gcgagtagta aaaaaagcaa
     5761 agcacctaac tgcaattttt cttactttat caatttcttt gccaaaagct ctggcacttc
     5821 cgctgggcgt tttgcaaccg gcacgtttgc ccttgaatag ttggcgactt ttgattctgc
     5881 agacccatag ttgccataca ctattgcacc agcatgaccc attctctttt cctttggagc
     5941 tgctcgtcct gcaatgtatg caataacggg cttgatgaaa tctgtcctta taatgtactg
     6001 agccaactgc tcctcggcgt ctccacctat ctcccctact accacgattg agtcaacatc
     6061 atctctgtct ctcactaggt cgaatgcttc aataagattt gtgccattga ttggatcacc
     6121 tccgattcca aggcacagcc tctgcccaaa gcccttgctt gataggttgt atgaaacatc
     6181 gtacatcaat gttccgctac gggaaaaaac tacagtggcc cctcccttga agggctgtgc
     6241 gggcatgatc cctaccttca ttacttccgg cacaatgaca ccgggggtgt taggcccaat
     6301 catctttgcg ccattttttc ttgcatactc taaaactttt attgcatctc ttattggaat
     6361 atgctcaggt attgcgacta gcaacttaat gccgctgtca attgcttcaa tggcagcctt
     6421 gaggaaaaat ggtgcaggaa caaaaattcc agatatttgt gcgctggtgg ccttaacagc
     6481 ttcactcatg gtattgtaca caggcgtacc ttcaaatctc tgccctccct taccaggggt
     6541 cacacctgca ggaatgtttg ttccatatgc tttcatgagc ctagtgtgtg tcgatccata
     6601 gctgccggtc atgccctgta ttatcactgg ttttctacca aagtcctcat ctccctcggt
     6661 tccaataagt gtgtcaaata tgttgaacgt tttttccact gttgttgtca ttttgatccc
     6721 ctgctactgt ttagagccga tactgcagac tgtattgcct cttccactgt attatagagc
     6781 tttgccttac tgccctggag catttccctt gccttctcag aatcagctcc agagatcctt
     6841 gcaaatacag gcacattcat aaggttgttg ttgtatgcat ctagtattgc ttgtgctaca
     6901 aggttggtcc ttacaatgcc cccaaagaga ttcacgagta ctacttaaac cctgttgatc
     6961 tt
//
LOCUS       DASQTF010000172        10140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_875264,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000172 DASQTF010000000
VERSION     DASQTF010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875264"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..905)
                     /locus_tag="VE308_04080"
     CDS             complement(420..905)
                     /locus_tag="VE308_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZB00184.1"
                     /translation="MEKQEEIIQAINDKFAEMSTETILFHQALADIVEIHITDHRCMY
                     FLHRYGAMPAGRLSELTGLTTAAVTGIIDRLEGAGYAKRTNDPKDRRRTIVEPIRNKK
                     LERRLETVFTPLHEKMHKLLSSYSDSQLAFLLDVMSKTIDLTREESKKLRHSMQGPSH
                     L"
     gene            1097..1582
                     /locus_tag="VE308_04085"
     CDS             1097..1582
                     /locus_tag="VE308_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00185.1"
                     /translation="MPIAEEGHSLFMSIFAPLRQAITSGHSSMSQMHGGPASSEESTM
                     EGMSESQMQGMNEGLIWNNILGSPATLPAGGLAVVVVISAIALAVAAFFVSRNQRSFV
                     VAGLLAASGVILMILALANMNFVIPGPIIGVVVGLAILGLGVAKGIRSARSTTVVARR
                     S"
     gene            complement(1805..1951)
                     /locus_tag="VE308_04090"
     CDS             complement(1805..1951)
                     /locus_tag="VE308_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00186.1"
                     /translation="MFKDPVCNMMVDEKTAKHVSEAGGGRKVYLCSAACKSQFDANPG
                     KYGY"
     gene            2169..2549
                     /locus_tag="VE308_04095"
     CDS             2169..2549
                     /locus_tag="VE308_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00187.1"
                     /translation="MAKITKSHSKKNLTLEATAAIAGVLLLAAVGTVSISSAQLQGQE
                     QEQQQQQQQQANSSAQNGTMTTTVVAVGGGGPSPVVMAFTLQSVTINAAKQSSGQIQL
                     SYPSLIRLALSDNKLTFPSWNRPI"
     gene            2531..2878
                     /locus_tag="VE308_04100"
     CDS             2531..2878
                     /locus_tag="VE308_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00188.1"
                     /translation="MESPYLIANGTELPPANPAEQNTEALVGRGQFDSPNNVIVAANS
                     GATNPVVIDAQNNVNYLQPNANYTMTGDELYVNSGFMWPEAAIPPGAPPITSFSITFE
                     RAGTYDYVCVIHP"
     gene            complement(3208..4182)
                     /locus_tag="VE308_04105"
     CDS             complement(3208..4182)
                     /locus_tag="VE308_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZB00189.1"
                     /translation="MQKGSQHSGHMMPAATDKNFRALKITALLTGIYFIIELGIGIYT
                     GSIAVLSDSFHTFSAVGGVLLALAAGRIAMKPADLSKTFGYKRAEVIGALLNGIFLLI
                     MAAIVFYMGFMRLQMPMELPPVPMLAAAFGGLVTEVIAIKLLYSGQKGDLNLKGAFWH
                     VLQTFVGSVIIIVTAVVIHFTGFMEIDPILGMAFGVVLTYASIGIVRDSFKILSQAVP
                     KDVDLEKVKDSLEAIQGVKNVHHIHAWSITPSVNIFSTHIHTDDLSKTDDILQKATRT
                     LKEKFGFYFSTIQVEKECAEIDEASDIDITKQARKKSNSTHWDNGSQH"
     gene            complement(4257..4439)
                     /locus_tag="VE308_04110"
     CDS             complement(4257..4439)
                     /locus_tag="VE308_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZB00190.1"
                     /translation="MLKDPVCNMIVDEKTAKYVSEVEGVKTYFCSAACKSEFEEDEKD
                     YNKNSSSSSNCCCCCY"
     gene            4560..6068
                     /locus_tag="VE308_04115"
     CDS             4560..6068
                     /locus_tag="VE308_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZB00191.1"
                     /translation="MMTTMSFKQQKIGDNNNKQESKIATQEHPHRWKALALLSLAQFL
                     IIMDTSIIGVALPAIQQQFSFSQSELQWIFSAYVIVFGALLLLGGRLSDIVGQRRIFV
                     IGFAILTASSVLAGLAPNGTVLIAARALQGIGAALIAPSAMSILMSLFTIPSERNKAM
                     GFWGAAAPAGGTAGVFLGGIITAYVDWSWVFLINVPIGIAVLALTWTLIPRGTRQKGR
                     VDYLGAVSITGALILLVYAIVTANDAGWTSVQTVSLLSTSAALLGAFTIIQKRSREPL
                     MPLRIFKTPNLLASNIVMALLGAAWIPMWFFLNLYLQQVQGYGAFESGLALLPMTAAI
                     MALMIGASSRLIRRLGLKHSLVAGLGLLAVAMLIFVATPSTKGSFATHVLPASLVAAA
                     GMSLAYIPALMSAISHAKKEESGLASGIVNTSYQIGSALGLAIMVAVASSQTLLDENN
                     GIGSIEALNNGFHLAFITAAIVAAIAAIVAVMAIKKHNTPGGKEEEIVSPTG"
     gene            complement(6359..6838)
                     /locus_tag="VE308_04120"
     CDS             complement(6359..6838)
                     /locus_tag="VE308_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HZB00192.1"
                     /translation="MPVDLDDIDVAILKSLMGDGRKSFRAISREIKVSTPTVKSRYER
                     LVNMGLIKSVKPEIDLSKVNREKKSQFFGEETIKRLEEQKKHFHVKIDNNMKVKMECE
                     YCGGPINGKPKVLEFANFQRFFCCIGCKNNYREKYAGRIQSIVEQYKEMQKEAKKLA"
     gene            complement(6985..7731)
                     /locus_tag="VE308_04125"
     CDS             complement(6985..7731)
                     /locus_tag="VE308_04125"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HZB00193.1"
                     /translation="MQSNNTPDLERPMLRMGSVAFLAGLVIALVSTLGFHPTGTGEEL
                     MNNPFIFGVYAEDPLWIASHIGQFAGILLIFAGGFVALHRLLVKSESATASALSWFGL
                     VTAIITAATFTILQAVDGIALKIASDTWYAIPPSEEERKTIYLGVAEGIRWNEWGLQA
                     YYRMLWGAVSLIFGVAIIKSTVLKKWIGAVGIAAGVVTIASGVVMAYVGFSSARDLVA
                     DLSTFIFYPWLVILGIFMWRKTREKKVITR"
     gene            complement(7922..8281)
                     /locus_tag="VE308_04130"
     CDS             complement(7922..8281)
                     /locus_tag="VE308_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00194.1"
                     /translation="MVAKGQSYQVTLNCEPEQLVASRNIKMTFEIKDANGKPICNLEP
                     LMAAGGRCVIIDADGSEFLHVHPAEEINGVVSWRGGPSVSFIAYFPKPGLYRAWGQFQ
                     HEGRLLTADFTFEVVAG"
     gene            8633..9094
                     /locus_tag="VE308_04135"
     CDS             8633..9094
                     /locus_tag="VE308_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZB00195.1"
                     /translation="MIPARQFDSVFDSFRREMERMMTRPWSIPTDWELPSMFEARDMR
                     MAPYELVDRGDSFELQLEIPGIEKENVDVKATKYSVEISGKHSEKGEEKGKRYVYSER
                     LYRSFYRNVPLPEEIIPSKVSAKVDNGILKLDLPKKNPTESESHATRVDVK"
     gene            9481..9786
                     /locus_tag="VE308_04140"
     CDS             9481..9786
                     /locus_tag="VE308_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00196.1"
                     /translation="MSVGGLSLGKIAIILIAAGVAIVFAISGFQARDIFFAPNITEET
                     QVIIKEQGTCTVQAEDDVPRTIGNCPYDVGDTVSITYKREQPPILEHQLIREQQQQG"
BASE COUNT         2951 a         2175 c         2078 g         2936 t
ORIGIN      
        1 tatttactgg gtggtatttt tgagtcaaat ctgtatcaga ttccaccgaa cccgctattt
       61 acaggggttc ttatactaca gtaaggaaag gaatagtaga tacaaaaatc gcaatgcacc
      121 aaagtgctgg aaaagcaata ataatagaca cccttaccgt ggctcctgga acaatactta
      181 caactgcaat acaagctaca aagcaaactc agcactagct aaaccctatg ctatacccct
      241 ttatgcacta aacagtccta actagagaga tattctacga gaatgcaata ttttttgcta
      301 aaattggttt gatgagtaat gctacaacga ataataaatc gataggggag ctttgcagga
      361 cctttgcatt gtcaacccgg actggttcaa aaaggtgatg tgctgtttaa gaagccactt
      421 catagatggg aaggtccttg catactatgc cgtaattttt ttgattcttc acgtgtcagg
      481 tctatagtct tactcatcac atcaagcagg aaagccaatt gactatcaga gtatgaagac
      541 aaaagtttat gcattttttc atgtaaaggt gtaaagactg tttcaagcct cctttccaac
      601 ttcttgtttc tgataggctc aacaatggtt cggcgcctgt cttttgggtc atttgttctt
      661 tttgcatatc cagccccctc gagtcggtca ataattcctg ttacggcagc agtagtcaaa
      721 ccagtcaact cagaaagcct tccggctggc atggctccgt acctgtgaag aaaatacatg
      781 catctatggt cagtaatatg gatctcaact atatctgcca gggcttggtg gaacaatatt
      841 gtttcagttg acatttctgc aaacttatcg ttaatggcct gaatgatttc ttcctgcttc
      901 tccatggctc tgaattattg ctatacagaa atagataaat atatttaagt tgtctaatat
      961 ctagatgtct tagataatga ccagtctagg aaatgtaaat acagggaact taagacacat
     1021 attatcagct ggattggccg cggtaacact tgccgtggtc ttgacttata tgctaggggc
     1081 cccggcgtta ttgcagatgc ctatagcaga ggaaggtcac agcctgttta tgtcaatatt
     1141 tgcaccgctg agacaggcaa taactagcgg ccactcatcg atgtcacaga tgcatggtgg
     1201 tccggcttcg tcagaggagt cgacgatgga aggaatgtca gagtcacaga tgcaaggaat
     1261 gaacgaagga ttgatatgga ataacatact tggcagcccg gcgacgctgc ctgcaggagg
     1321 ccttgcagta gtggtagtca taagtgccat tgcgctggca gtagcggcat tttttgtgtc
     1381 gcggaatcag aggtcttttg tggtggcagg attgctggca gcaagcggcg tcatactaat
     1441 gatacttgcc ctagcgaaca tgaactttgt aattcctggt cccataattg gagttgtcgt
     1501 tgggctggca atattagggc ttggagtggc aaagggcata agatcagcga ggtcgaccac
     1561 agtagttgcc agaagaagct gaggagcttg gctagatgtt tatcctataa acacatatgc
     1621 cattattgtt ctacaggcct catctgagaa caatacagcc cctcaataag gctctactat
     1681 tatcactttg ctatttgata ccagaacata acacctagag gaggagaatg atagaagtta
     1741 atggatgcat cagtttgcag ttagcactaa tgcactgttc attcaaggaa ataaacgact
     1801 cgctttaata accatatttt cccggattgg cgtcaaactg tgacttgcag gctgctgaac
     1861 agagatagac ctttcttcct cctcctgcct ccgatacgtg cttggctgtc ttttcgtcaa
     1921 ccatcatatt gcatacagga tctttgaaca tactgatatc ttgccgagcc ggatagataa
     1981 agacctcact ttacaagtgt aaaatcggat tatgagaagg tatgccggta tgccggttca
     2041 aggtctttca aaaccatact ctaacgtcat cggcttgtca gacgtttgag tgacttaaag
     2101 ttgatgatac ttatcctatt ctgctttgct tattaggcac aaagcttctt cagatgggct
     2161 gaaaatatat ggctaagata acaaaaagtc atagcaagaa gaatctgaca ttggaggcta
     2221 ctgctgctat tgctggagtc ctcctgcttg ctgctgtagg aacggtttct attagcagcg
     2281 cccaacttca aggacaggaa caagagcaac aacaacaaca acaacaacaa gcaaatagtt
     2341 cagctcaaaa tggtacaatg acgacgacag tagttgctgt gggtggcgga gggccttccc
     2401 ctgtggttat ggcatttacc ctacaaagtg ttactataaa tgcagccaaa cagtcgtctg
     2461 gacaaatcca actgtcgtac ccgagcctca tacggttagc tttatcagac aacaagctta
     2521 ctttcccctc atggaatcgc cctatttgat tgccaatggg acagagttac ctccagcaaa
     2581 tcctgcagaa caaaataccg aagcattggt aggacgagga cagtttgact ctccaaacaa
     2641 tgtcatagtt gccgccaaca gcggggctac aaatccagtt gtaatagatg ctcaaaacaa
     2701 tgtcaattac ctccagccca atgcaaacta cactatgact ggagatgaat tgtatgtcaa
     2761 ctcgggtttt atgtggcctg aagccgcgat tccaccaggt gcaccgccaa tcacatcatt
     2821 cagcatcaca tttgaaagag caggaacgta cgactatgtc tgtgtaattc atccctagat
     2881 gacgggtcaa gtagtggtgc agtaaaaagt agtcgaaatc aactattttg ttgtttcctc
     2941 atcccacttt ttcttttcct tcaatcaatt caatcaattt gctagctagt attatcataa
     3001 ttttttattg caatgacgac catgagagaa gaacagcgac acaccgttac tcaaattacg
     3061 cactaaagca acgttggtca cagaggctga gatactaaag gttgtattgc atttgctaga
     3121 gatagaagac gagaataaca attaccgttg tgctttttat tagtatccat ggcatactga
     3181 aaagtacttt tgtcgcagta ctgttactca gtgttgagat ccgttatccc aatgggtgga
     3241 atttgacttc tttcttgctt gtttcgttat atctatatca cttgcctcat ctatctctgc
     3301 acactctttt tctacttgga tagttgagaa ataaaatcca aacttttcct ttagcgtcct
     3361 agttgccttt tgtaggatat catcggtttt agatagatca tctgtatgaa tatgcgtaga
     3421 aaatatgttt acacttggtg ttatgctcca tgcatgtata tgatggacat tctttactcc
     3481 ttggattgct tctagggaat cctttacttt ttccagatcc acatcttttg gtactgcctg
     3541 agacaggatc ttaaatgaat ctcgcacaat gcctattgat gcatacgtga ggactacacc
     3601 aaaagccatg ccaaggattg gatctatctc cataaatcct gtaaagtgga ttacaactgc
     3661 tgttacgata ataattacac taccaacaaa agtctgcaat acatgccaaa aagcaccctt
     3721 taaattcaga tctccttttt gcccgctgta aagtaacttt attgcaataa cctcggtaac
     3781 gagccctcca aatgctgccg ctaacatggg tacaggcggc agttccattg gcatttgcag
     3841 gcgcataaat cccatataga agactattgc tgccatgatt aacaggaaaa tgccatttag
     3901 tagggcgcca atgacctctg ctcgcttgta tccaaaggtc ttagataagt cagcaggttt
     3961 cattgctatc cggcctgcgg ctaatgcaag caaaacccct ccaactgcag aaaaagtgtg
     4021 aaaagaatct gaaagtacag caatagagcc tgtgtagatg cctattccaa gctctatgat
     4081 aaagtaaatt ccagtaagca gagctgttat cttaagcgca cggaaatttt tgtcagtggc
     4141 agcaggcatc atatgcccag aatgttgcga tcctttctgc atattcgact tttcgtctcc
     4201 cctctacaaa aaactatcat acctctgcag acttcaacgg ccccatttat tggaactcag
     4261 taacagcagc agcagcaatt actactacta ctgctgttct tgttgtaatc cttttcgtcc
     4321 tcctcgaact cactcttgca ggctgcagaa cagaaataag tttttacacc ttcaacttct
     4381 gacacatatt tggctgtctt ttcatctact atcatattac acacggggtc tttgagcata
     4441 cccgacttag gtggaaatag atagataaag actaaagttt acaattgtaa ggctgtaaat
     4501 catgagtcaa attttacaat tgtaaaccta ccttgctata taactattca ttgaaccatg
     4561 tgatgacaac aatgtcattt aaacaacaaa aaataggtga taataacaat aagcaagaat
     4621 caaaaatagc tacacaggaa catccgcatc gttggaaggc attggcactg ctttcactag
     4681 cacagtttct aatcattatg gatacatcaa tcataggagt agcactacca gcaatacagc
     4741 agcaattcag cttctcgcaa tcagaattgc agtggatttt cagtgcatat gtgattgtgt
     4801 ttggtgcgct attgctgctt ggtggaaggc tttctgatat agttggacag cgtagaatat
     4861 ttgtaatagg ctttgcaatt cttacagctt cgtcagttct agctggatta gctcctaatg
     4921 gaacagtgct aatagcagca cgtgctcttc aaggaatagg tgccgcgctt atcgccccat
     4981 ctgccatgtc aatactaatg agcctattca ccatcccttc agaaagaaac aaggctatgg
     5041 gcttttgggg cgcagctgca ccagctggag ggacagcagg tgtcttctta ggtggcataa
     5101 taactgcata tgtagactgg tcatgggtgt tcctcattaa cgtgccaata ggtattgcag
     5161 tattggctct aacttggacg ctcattcctc gtgggacaag acaaaagggt cgagttgact
     5221 atttgggtgc agtatctatc actggagcat tgatcctttt agtctatgcg attgtaacag
     5281 caaatgatgc tggatggact tcagtgcaaa ccgtgtcttt gctctcgaca tctgcagctc
     5341 tattaggtgc atttaccata atacaaaaac gaagtagaga accactcatg ccactacgca
     5401 tattcaagac acccaatcta ttagcaagca acatagtaat ggctcttctt ggggcagcgt
     5461 ggataccaat gtggttcttc cttaacctat acttgcaaca ggtacaggga tacggagcat
     5521 ttgaaagcgg gcttgctctc cttccaatga cagcagcaat aatggctctg atgattggtg
     5581 catcttctag actgatacgc cgccttggat taaaacacag tttggttgca ggtttagggc
     5641 tactggctgt tgcaatgctg atatttgtag ctacaccctc tactaaggga agttttgcca
     5701 cgcatgtgct tccagcttcg ttagttgcgg cggcggggat gtccctagct tacatacctg
     5761 cactaatgtc tgctatatca catgccaaaa aagaagaatc aggtcttgca tctgggattg
     5821 taaatacaag ctaccagatt ggttcggcac ttggattagc aatcatggtt gcagttgctt
     5881 catcacagac attattagac gagaataatg ggattggctc aattgaggca ttaaacaatg
     5941 gcttccacct tgcattcata actgcagcaa tagtggcagc tattgcagct atcgtggcag
     6001 ttatggctat taagaagcat aacacgcctg gtggaaagga ggaggagata gtatcgccaa
     6061 caggctagta acaattccca gaagcaacaa attggtatgg cggattagac agacattcct
     6121 ttctacattc tttaatttaa tcaacaagga agcctgaatg cgttatcatt cgatatttgc
     6181 tttctagatc atgagagcgt atatacacat aattgctcct ttttttattt gctgctgatc
     6241 atcctcttta ctcgctagta caaaggccga attaggacca gtacaaagcc tgaattaagt
     6301 atttatttat taacagcagt tctttggtga tctcgaagta attagtttat tccaaaaatc
     6361 aggccagttt cttcgcctct ttttgcatct ctttgtactg ttccactatt gactgtattc
     6421 tacctgcata cttctctctg taattgttct tgcagccaat gcagcagaaa aaccgctgaa
     6481 aatttgcaaa ctctagtacc ttgggcttac catttatggg tccaccgcaa tattcgcatt
     6541 ccatcttcac cttcatgttg ttgtctattt tcacatggaa gtgcttcttt tgttcctcga
     6601 gcctcttgat ggtttcttct ccaaagaact ggcttttctt ctctctattg acttttgata
     6661 aatcaatctc tggttttact gacttgataa gccccatatt tacaaggcgc tcatatctgc
     6721 tttttactgt gggtgtgctg acctttatct cacgggaaat ggcacggaat gactttctgc
     6781 catcccccat caatgatttt aggattgcaa catcgatgtc gtcaaggtct actggcaacg
     6841 cgctgcaata tagttaatgc taacaggatt taattattat gaagaggagg tcaaagtatc
     6901 aatcttatat tttttctatt tctcttcttt tcgcataacg tggggcgtgg gggtacaagc
     6961 atttattatg ttccttctaa tatattacct agtgatgacc ttcttttccc ttgtttttct
     7021 ccacataaaa ataccaagga ttactagcca aggatagaaa atgaatgtcg agagatctgc
     7081 cacaaggtct ctagctgagg agaacccaac gtatgccata acgacaccag aagctatggt
     7141 aacgacacca gcagctattc cgactgctcc aatccatttc ttaagaacag tactctttat
     7201 gattgcaaca ccaaagatta gtgaaactgc cccccaaagc attctgtaat aggcttggag
     7261 accccactcg ttccacctaa tcccttctgc aactcctaag tagatggttt ttctctcctc
     7321 ttcactagga gggatagcat accaagtatc gcttgctatc ttgagtgcaa ttccatcaac
     7381 agcctgaaga atggtaaaag tggcggctgt gattattgct gttaccaagc caaaccacga
     7441 tagcgctgaa gcagtagctg attccgactt cacaagcaag cgatggagtg ctacaaatcc
     7501 accagcaaat atcaacaata tacctgcaaa ctgacctata tgggaggcta tccagagagg
     7561 atcttcagca tacactccaa agataaatgg attgttcatt agttcttcgc cagtacctgt
     7621 aggatgaaac ccaagggtag atacaagtgc aataacaaga cctgcaagga atgcaaccga
     7681 tcccatccga agcataggtc gctcaagatc aggcgtgttg tttgactgca tattgttttg
     7741 attaccaacc ttctttaagt attattttaa aaagtcttct agcagtctta tagtatcatt
     7801 tagcagctac aaaaccttgc atcagattac tttctctgct gtagcagcta caatccgatt
     7861 aaccgctatg ttacttcgct ttattattgc gagaaagatt cgattcaagt atgcaagttc
     7921 atcatcctgc aacaacttca aatgtaaaat ctgctgtcag aagccttcct tcatgctgga
     7981 attgtcccca agctctgtat agacccggct ttgggaaata tgctataaat gaaacagatg
     8041 gtcctcctcg ccagcttaca acaccgttga tttcttccgc aggatgaaca tgaaggaatt
     8101 cgctaccatc cgcgtcaatt ataacgcagc gacctccagc agccattaag ggttctaaat
     8161 tgcagattgg ctttccattt gcatctttga tctcaaaagt catcttgata ttcctacttg
     8221 ctacaagctg ctccggctca caattcaagg tgacttggta gctttgtccc ttggcgacca
     8281 cattttttac gcgggtttgc tcatgcgcaa taggagtccg agaatgaata gctgaccttc
     8341 aacattgaaa gcaaaggctg tcaggatttg ttgcattcca tctttgggtt tgacatcaat
     8401 ccacatcttg tatttaccag cttttgcgaa tgtgtgcatt atatggaaaa tacccgatca
     8461 tcaactgtgt tatttttgtg acccatacac tcaagatatc actgcaagga tgttataggg
     8521 ttaaggagta ctctgtcttg cactgcaatc aaaggcttca aataatatct cgacctccct
     8581 taattctacg agctcatcga gcgaaaggaa gcgtgaagaa gagaaaaagc ctatgattcc
     8641 agctagacag tttgacagcg tctttgacag ctttagaaga gaaatggaaa ggatgatgac
     8701 gcgcccttgg tctattccaa cagactggga gttgccctcc atgtttgaag caagagacat
     8761 gagaatggcg ccttacgaat tggtagacag aggagacagc tttgagctac aattggagat
     8821 tcctggaatt gaaaaggaaa acgtcgatgt caaggcgacc aagtactctg tagaaatctc
     8881 gggcaagcat tcagaaaagg gtgaagaaaa gggcaaaaga tacgtgtata gcgaaaggct
     8941 gtacaggtca ttctatcgca acgtgccatt gccagaggaa ataattccat caaaggtttc
     9001 tgccaaagta gataacggaa ttttaaaact tgatctgcca aagaagaatc caacagagag
     9061 tgagagccac gcaacgaggg tcgacgtaaa gtagcccctt tacaatcaca ctcatctctc
     9121 gccttttttc tactatgatc tatattatga catatgactg tcctaccctt tgcagtatgg
     9181 gttaaggcta taatttcaaa ttgaatgccc ggattaagtt gacatatttc atcatcatcc
     9241 tattgaagag tcaactgtcc ttctgtcaga cctctaaggc aagcatgaag agttgcaata
     9301 atgtagtttt gaattagtcc ttatggttga aatggaccct gtccaggata gaatgaatac
     9361 ccagagcagg catccattat atcttactaa aagctactat tactaggttc taaagaagag
     9421 tcgtaggatc gtagatatgt cagctgcaag cttattaatt ataggcatta cagtgcaaat
     9481 atgagtgtag gaggtctttc acttggcaag atcgcgataa ttttgattgc tgctggcgta
     9541 gctattgtat ttgccatttc aggctttcaa gcaagagaca tcttttttgc accaaacata
     9601 actgaagaaa cgcaagtgat aataaaagaa cagggaacct gcacagtaca agctgaggac
     9661 gatgtgccgc ggactatagg gaattgtccc tatgatgtcg gcgacacagt atccataaca
     9721 tacaagcgag agcagccgcc tatattggaa catcaactta ttagagaaca acaacaacaa
     9781 ggataacaac aacatactga aatagtctaa cgtccttgac aagcagattt tcttgtatgc
     9841 aaagctgatc tcacagagct aataaacagc ctacgagctc aatactacac tatttggtct
     9901 ctttttcttc ggtattgtaa gagggatagt tgaatataat catgcaattt gcaatataat
     9961 gcgattgcac tgaaagaatt tcatattact acgctacata ttttttattt gacaatagcg
    10021 tttcaatcgt gaacacatgt ataagtctag gactatagaa aatcaaatag ttagagatat
    10081 tgttggcgac aattgttcct acaatagctc caaatacgat tcagctaatt gtaagtgcct
//
LOCUS       DASQTF010000173         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_973723,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000173 DASQTF010000000
VERSION     DASQTF010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973723"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..303)
                     /locus_tag="VE308_04145"
     CDS             complement(22..303)
                     /locus_tag="VE308_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HZB00197.1"
                     /translation="MARLVARIRILPAEAESNLENVVNSIKKSMPQEMEMKSSSMEPI
                     AFGLKAIVGDFLLDDAEGQMDKLEESIKSVEGVGEIEVTNISRQSVRMK"
     gene            complement(306..485)
                     /locus_tag="VE308_04150"
     CDS             complement(306..485)
                     /locus_tag="VE308_04150"
                     /inference="COORDINATES: protein motif:HMM:NF011481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HZB00198.1"
                     /translation="MSKPLQLPVCSSCGRPIMPNDECVKYYCANCGYVLMWRCESCRE
                     FARPYKCIGCGFEGP"
     gene            complement(525..1691)
                     /locus_tag="VE308_04155"
     CDS             complement(525..1691)
                     /locus_tag="VE308_04155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZB00199.1"
                     /translation="MPHYDVVIAGGSISGLLAAREIAAGGLSVVVLEEDAEIGTPEHC
                     GGLVSISGIQNLGIVPDIDTIENNRIAHAKIFSPSSSFEISAERQKVIVLDRRALDKQ
                     IAFQAQKEGAEIRVKCSMRSFGNEGSEHVVKTSEGDLSCNFFVDARGIASIISRNRNG
                     VLHSAQYEVYASWIEPNTIEVVFDSQQYPGFFAWIIPTGRGRGKVGVAGRRVNAANAL
                     QTYIDSKGKYSVVRKVYAPIWISGPVRSFVSDRTIIVGDAAGQSKPTTAGGIYTCGMG
                     GLLAGRALVDAAKSDDRQKELLLRQYESKWLSMFKAEFDKMLLARRLLERIDNKAIDD
                     LITAIPTEIAQEASVHGDFDFHSTALAKILGAKGAARMAKALLGNEIRHLIPES"
     gene            1751..2113
                     /gene="pth2"
                     /locus_tag="VE308_04160"
     CDS             1751..2113
                     /gene="pth2"
                     /locus_tag="VE308_04160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HZB00200.1"
                     /translation="MRYVDLKQVIIVRRDVNMGTGKIAAQVAHAAVMGAEKVKTLRRE
                     WFNSWFVAGQAKVVVKVKNIEELMDVRRRAEELHLPVVQVQDSGLTQIPSGTITCIGI
                     GPAPSELIDKVTFELKLL"
     gene            complement(2110..>2727)
                     /locus_tag="VE308_04165"
     CDS             complement(2110..>2727)
                     /locus_tag="VE308_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZB00201.1"
                     /translation="LQQTAASTAAILTNGEIVFTIAISSIFFGEKPHGRAGLFAVILL
                     VIGLVIATTEDLKALETTLQLNAGNIMILASMLMWAIDNNVSRRLTSRVSPAKIAMVK
                     SLAGGLILTAIALALGKGSVIAGIKSDMWILIVGMSIAGFGGALLLFLEGIKRIGTVR
                     TMSMFSLTPIFGIVIAALALGESITVFQVIATGLIIVGIILIGRH"
BASE COUNT          714 a          679 c          568 g          766 t
ORIGIN      
        1 gctactatta gcaatattat attacttcat tcttactgac tgcctgctga tatttgttac
       61 ctctatctcg cctactcctt cgacactttt gatagattct tcaagcttgt ccatctgtcc
      121 ctcagcgtca tccaacaaaa agtcgccgac aattgctttc aggccaaagg caattggctc
      181 cattgaactg cttttcatct ccatttcctg tggcatggat ttctttatcg aattaacaac
      241 attttcaagg ttggactcag cctcagccgg aagtattctt atccgagcta ccaatcgtgc
      301 catggctatg ggccctcaaa accgcagcct atgcatttgt agggtcttgc aaactcacgg
      361 cagctttcac atcgccacat gagaacatat ccacaatttg cgcagtaata tttaacgcac
      421 tcatcattag gcataattgg cctgccacat gaactgcaga ctggcaactg caatggcttg
      481 gacataagtg atgcttctta agagtaaggg aatttatatc ttccttagct ctccggtatc
      541 aagtgcctta tttcgttgcc aagcaacgct ttggccatcc tagcagcgcc tttggctcct
      601 aggatcttag caagtgcagt cgagtgaaaa tcaaagtcgc cgtggacaga tgcctcttga
      661 gcaatctctg ttggtatagc cgtaatcaag tcgtctattg ccttgttatc tatccgctct
      721 aagagccggc gcgcaagtag catcttgtca aattcggcct tgaacataga caaccattta
      781 ctctcatact gtctaagcaa cagctccttc tgcctatcat cactctttgc tgcatctacc
      841 agagcgcgcc ctgctagcaa gcctcccatg ccacaagtgt aaatacctcc ggcagttgta
      901 ggcttgctct gtcccgctgc gtcacctact attatcgtcc tgtcagatac aaaacttcgc
      961 actggtccac taatccatat cggggcgtaa actttgcgaa cgacggagta ctttcccttg
     1021 ctatctatat aagtctgaag cgcgtttgca gcattaaccc tcctaccggc gacgcctact
     1081 ttgccccttc ctcgaccggt agggattatc catgcaaaaa aacctggata ttgctggcta
     1141 tcgaatacaa cttcaatggt attgggctct atccatgatg cgtaaacttc atactgtgca
     1201 gagtggagaa cgccattcct gtttctgcta attattgatg ctatacccct tgcgtctaca
     1261 aaaaagttgc atgaaaggtc tccttccgag gtcttgacta catgttccga tccttcgttt
     1321 ccaaatgatc gcattgagca ttttactctt atctcggcac cttctttttg tgcctgaaag
     1381 gcgatctgct tgtcaagagc gcgcctgtcc agcacgatga ccttttgcct ttccgcgcta
     1441 atttcaaaac tacttgaagg ggaaaagatc ttggcgtggg ctattctgtt gttttctatg
     1501 gtgtcaatat ctggcacgat cccaaggttt tgtatgccgg aaatgcttac aagcccacca
     1561 cagtgctccg gagtgccaat ttcagcgtcc tcctcaagta ctacaacaga aagacctcca
     1621 gcagcaattt cacgggcagc aaggaggccg gagatacttc cccctgcaat taccacatcg
     1681 taatggggca tctggcagat gactagtaat atatttttca gtctcttaat taaactatga
     1741 gcaagcaggt atgagatatg tggaccttaa gcaagtaatt attgtcaggc gtgatgtcaa
     1801 catgggaacc ggcaagatag ccgcccaagt tgctcatgct gcagtcatgg gggcagaaaa
     1861 ggtaaagacg ttgaggaggg aatggttcaa ctcatggttt gtcgccggcc aggccaaagt
     1921 tgttgtgaaa gtaaagaata ttgaagaatt gatggatgta agaaggcgag ctgaagagtt
     1981 gcacctgcct gtcgtgcaag tgcaagatag tggtttgacc cagataccat caggcacgat
     2041 tacttgcatc ggaataggac ctgctccttc agagcttatt gacaaagtga catttgagct
     2101 caagctcctc taatgtcggc caatcaggat tatgcctaca attatcaagc ctgtcgcaat
     2161 aacctgaaaa acagttatag attcgccaag tgccagtgcc gcgatgacta taccaaaaat
     2221 aggagttaat gaaaacattg acatggtcct tacggttcca attctcttta tgccttcgag
     2281 gaataacagt agcgccccac cgaaccctgc aattgacatt ccaactatta ggatccacat
     2341 atcagatttg atccccgcga tgacacttcc tttaccaagt gccaaagcta ttgcagtcag
     2401 aataagaccg ccggcaagtg acttaaccat cgctatcttg gcagggctta ccctagatgt
     2461 gagacgcctg ctgacattat tgtctatcgc ccacatgagc attgatgcca gtatcatgat
     2521 gttaccagcg ttcaattgaa gagtggtctc taaagctttc agatcttctg tggttgctat
     2581 aactagacct atcactaata aaattactgc aaataatcca gcccttccat ggggtttttc
     2641 cccaaaaaat atagatgaaa ttgctatcgt aaatacgatt tcaccgtttg tcaatatggc
     2701 tgccgtagaa gcagctgttt gctgaag
//
LOCUS       DASQTF010000174         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_427307,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000174 DASQTF010000000
VERSION     DASQTF010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427307"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VE308_04170"
     CDS             complement(<1..269)
                     /locus_tag="VE308_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00202.1"
                     /translation="MIKTHKDLAAIDTNKRQENLKLQSKKIHLRNNNSNSSNSNDSRL
                     ASIRGANNAKRPECTFRMEDYLETIYELVQHKGYATLADIAEYLNV"
     gene            complement(266..1588)
                     /locus_tag="VE308_04175"
     CDS             complement(266..1588)
                     /locus_tag="VE308_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation transporter"
                     /protein_id="HZB00203.1"
                     /translation="MEKDEIHRSSSSLQLRWFANAKLVAVALVPLVVLAAMIAFIFGP
                     GQQFLGAGTPLPEISIERIEFQESGTVIAFIRNTGPIDVTVAQADVNDRILPAAIEPG
                     KELPRLAEAKVIIPFPWNEGEPYEIGVTSSDGTRFSKSVNAAAPAPTPDIEQAAFFGL
                     IGTYVGIIPVLAGLLWLPFIKRLSLQKYRFFLSLTAGLLVFLGIDALVEGSEIAAESV
                     AGVFNGQILIVLVTILSFLALMYASAKLGERSKGRRAEEADEFSSYDSIHASQAKTLD
                     QQQRHHLVVKPVAMALMISIGIGLHNFGEGLAIGASVVLGEIALSTFLIVGFMIHNTT
                     EGLAIVSPLARTRVRRMVGKMIIMGLIAGVPTIFGTWIGGFVYSPIATIIFLSIGAGA
                     IFQVVFLIYQGMAKAEAEKSKIISDASIAAGFAAGMLIMYVTGLMIPS"
     gene            complement(1591..>2656)
                     /locus_tag="VE308_04180"
     CDS             complement(1591..>2656)
                     /locus_tag="VE308_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZB00204.1"
                     /translation="QSFVDFLATAQDAELRKNTIGGHTAGYGVHETSGDVLDPVSSSN
                     TASEEVVMDPDTYLRSFNYGRVSNLANGSSVREFTLVADDRQMMEISPGVFYNVWTFN
                     GTVPGPTLRATEGDLIRINFINNGSKPHTIHTHGIHKAEMDGVFEIINPKGRITYEFY
                     AEAFGVYPYHCHVTPLEEHISHGLYGVYIVDPKTSRPEADEMVMIMNGFDTDFDTENN
                     FYAVNTIPYYYMHHPIEIEEGKLVRVYLVNMLEFDQINNFHLHGNLYQLYRSGTSMGP
                     DEYTDMVTMSQGERGILEFSYKYPGQYMFHAHKTEFAEKGWMGLFMVKDPAETRSDGS
                     SSNSSGISLETKTISGDVGT"
BASE COUNT          699 a          617 c          545 g          795 t
ORIGIN      
        1 acattaagat attcggcaat atcagccaaa gtggcatagc ctttatgctg aacaagctca
       61 tagatagttt cgaggtagtc ttccatccta aatgtgcact ctggtctttt ggcattgttt
      121 gcgcctctga ttgaggcaag cctgctatca ttagaattgc tgctattgct gttgttattt
      181 cttaaatgaa tcttttttga ctgaagcttt aggttttctt gcctcttgtt agtatcaatt
      241 gctgctaaat ccttgtgcgt tttaatcatg atggaatcat caatcctgtt acatacatta
      301 ttagcatgcc tgcggcaaat cctgcagcga ttgatgcatc tgaaattatc ttgcttttct
      361 cagcctctgc cttggccatc ccctggtata tcaaaaatac aacttggaat attgcacctg
      421 ctcctattga taggaatatt atcgtagcaa taggagaata tacaaatcca ccgatccagg
      481 tgccaaaaat tgttgggacg ccagctatta gacccatgat aatcattttg cctaccattc
      541 ttcttactct tgtcctggca agtggagaga cgattgcaag accttctgtg gtattatgaa
      601 tcatgaaccc tactatgaga aatgtactta gcgcaatttc tccaaggaca acagaagctc
      661 ctatggctag cccttctcca aagttgtgaa gtccaattcc tattgatatc atcaatgcca
      721 tcgctacagg cttgacaact aggtgatgac gttgctgctg atccaaagtt ttcgcttggc
      781 ttgcatgtat agagtcatat gaagaaaatt catcggcttc ttctgctctt ctgcctttgc
      841 tgcgttcgcc tagcttcgca gaagcataca tcaatgcaag aaatgataat attgttacca
      901 ggacaatcaa aatctggccg ttgaagaccc ctgctacgct ctctgcagcg atctcactac
      961 cctcaactag ggcatcaatt cccaaaaaga ctagcaaacc agcagttagg ctgagaaaga
     1021 atctgtactt ctgaagactg agcctcttga taaacggaag ccacaagagt cctgccaata
     1081 ctggtatgat acctacatag gttccgataa gcccaaagaa tgctgcctgt tctatatccg
     1141 gagttggagc aggagcagct gcattgacac tcttgctaaa cctagtgcca tccgagctag
     1201 tcacacctat ctcataaggc tctccctcat tccaaggaaa tggaattatc actttggctt
     1261 ctgcaagcct aggcaattct ttgccaggtt ctattgcagc tggcaggatt ctgtcattga
     1321 catctgcttg ggctactgta acatcgattg ggcccgtgtt gcgtatgaac gcgattactg
     1381 ttccactttc ttgaaactct attctctcta tgctgatttc aggaagagga gtacctgcac
     1441 caaggaattg ctgacctgga ccgaagataa aggcaatcat tgctgctaaa actactagag
     1501 gaaccaatgc aaccgccacc agttttgcat ttgcaaacca gcgtagttgt aatgatgatg
     1561 atgatctgtg aatttcatct ttttccatgg ttaggtgcca acatctcccg atattgtctt
     1621 ggtttcgagg gatatgccag aactattact gctgctgcca tctgatcgtg tttcggcagg
     1681 atccttaacc atgaaaagtc ccatccagcc tttttctgca aactcagtct tgtgtgcatg
     1741 gaacatatac tggccaggat acttgtagct aaactcaagt atgccccgct caccctggct
     1801 catagtcacc atatctgtgt attcatcagg ccccatactc gtaccacttc tgtacagctg
     1861 gtataggtta ccgtgcagat ggaaattgtt tatctggtca aattccagca tgttaacaag
     1921 gtataccctg acaagtttgc cctcctctat ctctataggg tgatgcatat agtagtacgg
     1981 aatagtgttt acggcataga agttattttc agtatcaaag tctgtgtcaa agccattcat
     2041 tatcatgacc atttcgtcag cttctggtct agaggtcttg ggatcaacga tgtatacacc
     2101 atataggcca tgcgaaatgt gctcctcaag tggggtgaca tggcagtgat atgggtaaac
     2161 tccaaaagcc tcggcgtaaa attcgtaagt gattctcccc ttaggattaa tgatttcaaa
     2221 cactccatcc atctctgcct tgtggatacc gtgtgtatgt atcgtgtgag gttttgagcc
     2281 attgttgatg aaatttatcc ttatgagatc gccttccgta gcccttagag ttgggccagg
     2341 tacggtacca ttaaaagtcc atacattata gaatacacct ggtgatattt ccatcatctg
     2401 cctatcgtct gcaacaagag tgaactccct tacagaggag ccgttagcaa gattggaaac
     2461 gcgcccatag ttaaaggatc tgagataggt gtcagggtcc atgaccacct cttcgctggc
     2521 agtattggaa ctacttacag ggtctaaaac gtctccgctc gtctcatgta ctccataacc
     2581 tgccgtatgc cctccaatag tattttttct taattcagca tcctgtgctg tggcgaggaa
     2641 atcaacaaaa gattga
//
LOCUS       DASQTF010000175         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_974925,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000175 DASQTF010000000
VERSION     DASQTF010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974925"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VE308_04185"
     CDS             <1..921
                     /locus_tag="VE308_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase"
                     /protein_id="HZB00205.1"
                     /translation="LYSRFGFIDEIVEVGSFAEILGDMCRSRLKQAGSVLIPHGTLIS
                     QMKSDEIEKIDTPVFGNKWILRWEADRDLKERLMKEARLKTPQTVRSKNEIKGLCIVK
                     LHGAAGGRGYYLAWDCESFEEGARRLINQKIIREESDLYIQEFIRGVPVYLQYFYSPI
                     TEELELLGIDRRYESDIDGIGRIPAKQQLGAGSIEPSYTVIGNIPLVLRESLLEEVYK
                     IGENFVHASERLVPPGMPGPFCLEGVYDSEGKFTTFEFSARIVAGTNLYLDGSPYSGL
                     LYDEPMSMGRRIAREVKIAKKKDELDKVVT"
     gene            1111..2259
                     /locus_tag="VE308_04190"
     CDS             1111..2259
                     /locus_tag="VE308_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00206.1"
                     /translation="MTTTKKSNNSAFYVSYVSYVIAAAAILMAAGVTGSALLTPHQAI
                     ASIDNTIEEVERVSVGGILNGNLTVSVNQFSPAAVEIQTGESVRFYAPDNSTEVHNVI
                     FDMTNGSTISGLELPFILPQGADPEQLELALPFNFGESIIQEQPDGMQAIISLNKGAY
                     YPTVADQENNTRYLIDVEEYQQLIEEARQQGFFEPQNLSANYTMNGTEAIVSSGIILD
                     VGGFDALFEEEEGEEANMTAANDAIDINATTDAATTPIPPAEIGQENATTAAAEQEGG
                     GGEEEFPPLPYPILDSFTVTFEEAGTYEYFCAFHPGMYGIVTVAGEEGEEAGEEGEVS
                     AENLTTTTTTNDTDSNATTTVVTTTPIPPAEIGQENATTTAVTEEEQQ"
BASE COUNT          899 a          530 c          636 g          603 t
ORIGIN      
        1 ctatactcaa ggtttgggtt tattgatgaa attgttgagg ttggcagttt tgccgagata
       61 cttggtgata tgtgtcgcag tcgcctcaag caggcaggct ctgtactgat tccccatggt
      121 actttgatat ctcagatgaa aagcgacgaa atcgagaaga tagatacacc agtctttggc
      181 aataagtgga tattaaggtg ggaagcagac agagatctca aagaaaggct gatgaaagag
      241 gcacgcctga agactcccca gactgtcagg tcaaaaaatg agatcaaggg gttatgcatt
      301 gtgaagttac atggagccgc cggtgggcgg ggctattacc ttgcatggga ctgcgagagc
      361 tttgaagaag gtgcaagacg gctcattaat caaaagataa tcagagaaga aagcgacctt
      421 tatattcagg aattcattag aggtgtgcca gtctatttgc agtatttcta ttcaccaata
      481 actgaagaac ttgagctgct gggaatagac aggcgatatg aatcagatat tgacggcatc
      541 ggtaggatcc ccgcaaagca gcagctcggc gccggcagca tcgaaccgtc gtacactgtc
      601 attggaaaca taccgcttgt tctgcgtgaa tcattgcttg aggaagtata caagataggt
      661 gaaaactttg tgcatgcttc agagcggcta gtgccaccag ggatgcctgg accgttctgc
      721 ctagaaggag tatatgatag tgaaggcaaa ttcacaacct ttgaattttc agcaaggatc
      781 gtggcaggga cgaacctcta ccttgacggt tcaccttact ctggactatt atatgacgag
      841 cccatgagca tggggagaag aatagcacgc gaagtaaaga tagcaaagaa aaaggacgaa
      901 ctggataaag tagtaactta agagggtttc ttatgaagat atagggcgag taccaataaa
      961 gggagacttg tatggcgagg gaaaatttta acccaagacg gaggttcatg aattgtgagc
     1021 aacggggcca ccaaaaaaga ttaccaggaa agattgaaag gtagatcaca acaatcctta
     1081 aaatgccctc attcctccac accaatatca atgactacga cgaaaaagag taataatagt
     1141 gcattttatg tgagctatgt gagctatgtt atagccgctg cagcaattct tatggcagcc
     1201 ggtgtaactg gttctgcatt acttacacca catcaagcaa tagcatcgat tgataacaca
     1261 atagaagaag tagaaagagt ctcagtagga ggaatactaa atggcaacct gactgtatct
     1321 gtcaaccaat tctcacctgc agcagtagag attcaaacag gagaaagtgt tagattctat
     1381 gcaccagata attccacgga agtacataac gtaatatttg acatgaccaa tggatcaaca
     1441 atatcgggtt tggagttacc attcatactg ccccaaggag ctgacccaga acaactagaa
     1501 cttgcacttc cattcaattt tggcgaatcc ataatccaag agcagccaga tggcatgcag
     1561 gctataatct ccttgaataa aggtgcatac tatccaaccg ttgcagacca agagaacaac
     1621 acaagatatc tgattgatgt agaagaatac caacagctaa tagaggaggc aaggcagcaa
     1681 ggattctttg agccacaaaa cctatcagcc aattatacaa tgaatggaac tgaggcaata
     1741 gtcagttcag gtataatact agacgtcggt ggttttgacg ccctttttga agaggaagaa
     1801 ggagaagaag caaatatgac ggcagcgaac gatgctattg acatcaatgc aacaacagat
     1861 gccgcaacaa caccaatccc tcctgcagag ataggacaag aaaatgcaac aacagcagct
     1921 gctgagcaag aaggaggagg aggagaagaa gaattcccac cgcttccata cccaattcta
     1981 gatagcttta cggtgacgtt tgaagaagct ggcacctatg aatacttctg tgcattccat
     2041 cccgggatgt atggaatagt gacagtagca ggagaggaag gagaagaagc aggagaggaa
     2101 ggagaagtat cagcagagaa tttgacaacg acgacgacga cgaatgatac tgactccaat
     2161 gcaacaacaa cagtagtaac aacaacacca atccctcctg cagagatagg acaagaaaat
     2221 gcaacaacaa cagcagtaac tgaggaagaa caacaataac aacagcaaac aactgcaata
     2281 ccagcatcgt tgctggaata acaaagatag catagggagc aatagaagtt ttcgcttacc
     2341 taacttttct tttttatttt attaagaaag tatgcagctc tgtgctaaat taaaagttcc
     2401 aaacactata ggatttagca acagaggggt gaaaggagag gagattcatc atgcattgag
     2461 gaagcaaaca atatgttaca ttagcacgcc taaataatat gcccacctgt gcatcgcagg
     2521 cattgtagtg caaaagcttt aaagcgcatc gatgggttgt caagttttgt gcaagacatc
     2581 aagctggttt attctaccac tgaccggcca aagctcaagt caccacatct tatctgcgga
     2641 ttccctggca gcggttatgt tggcaagc
//
LOCUS       DASQTF010000176         4403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1076530,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000176 DASQTF010000000
VERSION     DASQTF010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076530"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /gene="nrdD"
                     /locus_tag="VE308_04195"
     CDS             complement(<1..1511)
                     /gene="nrdD"
                     /locus_tag="VE308_04195"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HZB00207.1"
                     /translation="MASPNRIDILRILNAKGPLTYSELKALAGFKSKKESGKFAYHLR
                     KLLRQLLVALNKTERRYTITNLGKLVLSLARQIEERSIIESGKMYVRTNKHTIEEFNS
                     HKIIQSLVREANLPLEQAHKITEEAENKIYRFQTTYLTSSLIRETVNCILIEHGHEEY
                     RNKLARLGLPSSDIVQMLSQDEPARNGIERVMTRTAGAVFSEYLLFNILPKDIADMHL
                     AGEIHIPNTHVWGLLPDTIFIDLSELKDGLDLKGKFLNMARIPPIKSSDDAVATLPAL
                     VSLLSREASTEVVLEGYVAALLNNVKDPEDIASRFARALLASSGAPSYSTGMPVTTIA
                     VQTDSLDAQQLDALLDGYRRYVNNTPMPRLGLALAGRVKDSLDHITAAVRSGGIISIG
                     NKVRASNGIKKADAKATSTMSLHLLSINLPRLAYESNKDETYFRAKLALMIKPSLAAM
                     SLRKKIIVDYVKKGMMPAFASVSQLMQMSTASIAVNLTGIRESVYNILGESSS"
     gene            complement(1709..2317)
                     /locus_tag="VE308_04200"
     CDS             complement(1709..2317)
                     /locus_tag="VE308_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00208.1"
                     /translation="MTGGEKRQQFMSQITHATGVVYNQLGRLKLLDRRFASMETSFLE
                     QIASNIKAGNNARAKIIATELINVRRLRRNTQHTGLALEAVVIRFSTINEFAAILDTI
                     DPTIEMIKGIQTDLTRAIPAANEVLSEVSTVTADVLLNANIKTEARISTPMDTEALSI
                     LHQIEGALENEAKARLPEVPASIPSQKRENAPTEKTQVLVET"
     gene            complement(2539..2682)
                     /locus_tag="VE308_04205"
     CDS             complement(2539..2682)
                     /locus_tag="VE308_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00209.1"
                     /translation="MPIAQEIIVILLRLDVGADEHILYKPIQIQVLHSVAEKLVANTN
                     AIP"
     gene            complement(2783..3175)
                     /locus_tag="VE308_04210"
     CDS             complement(2783..3175)
                     /locus_tag="VE308_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZB00210.1"
                     /translation="MIEEKLSTHKSTVEKFIEAANRKKILAQKCVKCGHLMLETVFFC
                     EKCAGNKFEQVDLEGSGVVVTYTIQAVVPEGFEDVGSYAWVVFKLDNAPLRASGLLPG
                     IKTPADLPIGTKVRVANYDPKHGLMLQK"
     gene            complement(3172..4341)
                     /locus_tag="VE308_04215"
     CDS             complement(3172..4341)
                     /locus_tag="VE308_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HZB00211.1"
                     /translation="MTTNKVCLLGAGSTKYGKLNESIIEIALNAAKDAVESAGITPKE
                     IQAGYISNVFGVADKQVHMAPVIMSNLGIPNVPGLTIESACGSGSVMFREAFANVAAG
                     FYDCVLALGVEKVTHTGTARSTTLFSYCSDFFYEGGNGASFPGLFASMARAYMTTHKA
                     TEEDLAHVAVKNHENGLMNPKAHVHKKITVDDVLKSPVVASPLKMLDCCPFSDGASGV
                     ILCSEEFAKKSGRPYIEVIGSGRGASPAAVQGREDITTIPSTVAAARQAYKMAGVSPK
                     DIDFAEVHDCFTIAEIIDIEDLGFFPKGKAVHAVRDGATRLNGEIPVNPSGGLKSKGH
                     PIGATGVGQVVEVFEQFTGKAGERTVPNSEIALTHNFGATGASAAIHIFKKVDRK"
BASE COUNT         1079 a         1063 c         1009 g         1252 t
ORIGIN      
        1 tcgctagagg actctcctag tatgttgtag acggattccc taatgccagt aaggttgact
       61 gctatgctag ctgtactcat ctgcatcaac tggcttacac tcgcaaatgc aggcatcatc
      121 ccctttttga catagtcgac gattattttt tttcggagcg acattgcagc aagcgacggt
      181 tttatcataa gcgcaagctt ggcgcggaag taagtttcgt ccttgttcga ttcgtatgca
      241 agccttggca ggtttatcga aagcaaatgg agcgacattg tagatgtagc tttggcgtct
      301 gccttcttga tgccattgct tgcccgcacc ttgttgccaa tcgaaattat gcctccgctt
      361 cgtactgctg ctgtaatatg gtcaaggctg tccttcaccc tgcctgccag cgcaaggcca
      421 agccggggca taggcgtatt attgacgtac ctcctgtagc cgtccaagag tgcatcgagc
      481 tgctgggcat ctaagctgtc agtttgcact gcaatggtgg ttacaggcat gcccgtagaa
      541 tacgagggcg cacctgatga ggctagaagt gcccttgcga accgagaggc aatgtcctct
      601 ggatctttga cgttattaag taacgccgcc acgtagcctt cgagcacaac ttctgttgac
      661 gcttctcttg aaaggagcga gaccaatgcg ggcagggttg cgacagcatc gtcagagctc
      721 ttaatcgggg gtatcctagc catattcaag aatttccctt ttagatccaa gccatctttc
      781 agctctgaca ggtcaatgaa tatagtatcg ggcaataaac cccagacatg ggtattagga
      841 atatggatct cgcctgccaa atgcatatct gcaatgtcct ttggcagtat gttgaacagt
      901 aagtattcag aaaagaccgc gccagcagtc cttgtcatga cacgctctat tccgtttcgg
      961 gctggctcgt cttgggagag catctgaaca atgtctgagg atgggagacc cagccgcgct
     1021 agcttgtttc tatattcctc gtggccgtgc tctatgagaa tacagttaac ggtctcacgt
     1081 atgagcgatg atgtgagata ggttgtctgg aaccggtaga tcttgttctc tgcttcttca
     1141 gtgatcttgt gggcctgctc aagcggcaga ttggcttccc ttacaaggga ttggattatc
     1201 ttgtgggaat tgaactcctc aattgtatgc ttgtttgtcc tcacatacat cttgccgctc
     1261 tctatgattg agcgctcctc gatctgacgt gcaaggctca gcaccagttt accaaggttt
     1321 gtgatcgtgt accgacgctc cgtcttgttg agggcaacca gcagctggcg aagcagcttc
     1381 ctcagatgat aggcaaactt gcccgactct ttctttgatt taaaacccgc aagagccttt
     1441 agttccgagt atgtcagggg gccctttgca ttcaaaattc gcagaatgtc aattctgtta
     1501 gggctagcca taacagagaa aatcatcctt acgcgcttgg acgcagattc taaaagtccg
     1561 cctttcttag aatctttttg ataatcaaaa ctcggttgtt ccattacgcc tcatatctag
     1621 ataaatgatt aaaactactt tgtaaaatat ttttaactaa ttgcatgatc cactctatgt
     1681 tgctcaatcg acgttttggt cgaccagtct aggtttcaac cagtacctga gtcttttcag
     1741 ttggggcatt ctctcgtttt tgagagggaa tgctagctgg aacctcaggg agtctcgctt
     1801 ttgcctcgtt ctcaagtgct ccttcgatct ggtgaaggat tgaaagggcc tctgtgtcca
     1861 tcggagtcga gattctggcc tctgtcttga tatttgcgtt gaggagcacg tctgcggtga
     1921 ctgtagagac ttcagagagt acttcattag cggctggaat ggctctagtc agatccgtct
     1981 ggataccctt tatcatctca atcgtcggat ctatggtgtc aaggattgca gcaaattcgt
     2041 tgatcgtact gaagcgtatc actactgcct caagtgctag gccggtgtgt tgtgtgtttc
     2101 tacgtaggcg ccgcacattg atcagttcgg ttgcaatgat cttcgctcgg gcattattgc
     2161 ctgccttaat gttggaagct atctgctcta gaaaagatgt ctccattgag gcgaaccttc
     2221 tgtcgagcag cttgagacgc ccaagctggt tgtacacgac acctgtcgcg tgagttatct
     2281 ggctcatgaa ctgctgacgc ttctctcctc ctgtcatctt tctgttatta cgctagatta
     2341 aatgaaaaaa ccatagatgt cgaagacagt ataccaaaaa ctatcataaa gctgagctac
     2401 aaaaaaattt gtattttaca gtagcaatgc aagaggtata caagaatcaa gatgttcgcc
     2461 agtgatgatg atgttcatag ccgactctta aaggcaaaac ggtcgagatc gagcacgcaa
     2521 gagggctcaa gctaagtcct acggtattgc atttgtattt gccaccagct tttcagctac
     2581 ggagtgcagt acctgaattt ggattggttt gtacaatatg tgctcgtcag cgcccacatc
     2641 tagacgaaga agaataacaa taatttcttg cgcgattggc actagtgtat gctagattca
     2701 ttcaagcaga tatacaaaat gatataaaat atacagttgg aattataaga aaagatatac
     2761 gtgttctagg caacaggagc aactatttct ggagcataag accgtgcttg gggtcataat
     2821 ttgcaaccct caccttggtg cctatcggta ggtcggcagg cgtcttgatg ccagggagaa
     2881 gtcctgacgc tcgtaggggt gcattgtcta acttgaacac cacccatgcg tacgagccta
     2941 catcctcaaa accttccggc acaactgctt gaatggtgta tgtcacaaca acgccactgc
     3001 cttcaagatc aacttgctca aatttgttcc ctgcacattt ttcacaaaag aatacggtct
     3061 ccagcatcag gtgaccgcac ttcacgcatt tttgagctaa gatctttttc ctgtttgcag
     3121 cctcgatgaa cttttctacg gtacttttat gagtcgataa tttttcctct atcatttcct
     3181 atccactttc ttgaatatgt gaattgcggc gctagcacca gtggccccaa agttgtgagt
     3241 gagggcaatc tcggaatttg gaactgtacg ctcaccggct ttaccagtga actgctcaaa
     3301 tacttcgacc acttgcccga cacctgttgc accaatcggg tgcccttttg atttcaagcc
     3361 acctgatgga tttaccggga tctcgccgtt aagacgtgtt gctccgtccc ttactgcatg
     3421 aacagccttg ccctttggga agaacccaag atcttcaatg tcgattatct ctgctattgt
     3481 aaagcaatca tgcacttcgg caaagtcaat atcctttgga ctcactccgg ccatcttgta
     3541 ggcctgcctt gcggcagcca ctgtacttgg aatggtcgta atatcttcgc gcccttgaac
     3601 agctgctggc gaagcgcccc tgccagatcc aattacttcg atgtatggcc tgccgctctt
     3661 tttcgcaaac tcttcgctac atagaataac tccagatgca ccatcagaga acgggcagca
     3721 gtcaagcatc ttcagcggcg aagccaccac cggtgacttt agcacatcgt cgactgttat
     3781 tttcttgtgg acatgcgcct ttgggttcat caacccattt tcatggttct tgaccgcaac
     3841 gtgagcgagg tcttcttcag ttgccttgtg ggtagtcata taagcgcggg ccattgatgc
     3901 aaagagccca ggaaatgacg cgccgttacc accttcatag aaaaagtcag agcagtaaga
     3961 gaacaaggtc gtgctacgcg ccgtgccagt atgcgtcacc ttttcaacac caagcgcaag
     4021 gacgcagtca taaaatccgg ctgctacatt agcaaatgcc tctctaaaca tcacggagcc
     4081 ggagccacac gccgattcaa ttgtaaggcc aggaacatta ggaattccaa gattactcat
     4141 gatgacaggt gccatgtgga cctgcttgtc tgcaactccg aaaacgtttg aaatgtatcc
     4201 agcctggatc tcttttggag taatcccagc agactcaacg gcatctttgg ccgcatttag
     4261 cgcaatctct ataatgcttt cgtttaattt tccatatttc gtgctgcctg caccaagaag
     4321 gcaaactttg ttggttgtca tctcataaaa attgcttttt tgtatataaa tagcttaaac
     4381 acccttgata tataagaatc ata
//
LOCUS       DASQTF010000177         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1247746,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000177 DASQTF010000000
VERSION     DASQTF010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247746"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            550..966
                     /locus_tag="VE308_04220"
     CDS             550..966
                     /locus_tag="VE308_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00212.1"
                     /translation="MENKIESAMEIEEAGNYLQLSFSRAPKKNQEALAQLGKRWVQWL
                     NKHGVSSKIYYLNSSTTTNEQVPEGVESIAKILSVSDDEMLGVSLQFYRDQAHANEVY
                     SKMMQDETCGAIGKEFDGLVTQGKGMITDGFSRLRV"
     gene            complement(1178..1810)
                     /locus_tag="VE308_04225"
     CDS             complement(1178..1810)
                     /locus_tag="VE308_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00213.1"
                     /translation="MSLGVLLLLPASLGFGGTIIQEAFGQLDLPPLDGGQEDGGGEVE
                     ATLPSTTTTFLLRGIIGSSLPAPGGDIPTENSSYILAGRWRIFVDEGLVNRFVAEMDL
                     AATNGTAIHNITIEDTTPHRFELTESGNGMSTTAATGSIPPVSSNLMTRISVNGNTPI
                     IDNVPMTVSIRGQVLAIEDIDIDETTITDTGQLDILSIIDGQSIFGVVPR"
BASE COUNT          589 a          412 c          428 g          599 t
ORIGIN      
        1 gttctgactc taagtaattg tagagttagc atagtctact ctctataaag tagatgtgtt
       61 gctatggtta ttttaatgtc aagcattcta tggcagatgc agcttgatct aagtataagc
      121 aaaatcggtt tgttagtccg atatgccgtg taaaggatgt atatacatga tggtcgcatt
      181 tggttggttg cttgccacaa tacacaaaag gcagagatac gtgagatgaa gttactaact
      241 gtgatcggtc ggggtcataa aaaacactca cgcacagcgg tgtaggtgtg taattttata
      301 ccagaccgga atcacagcga aaaaataagt tgtctgtttg tgtctcagtg tgtgtaagaa
      361 gctaaagttc gtggaacaag tgaaaacccc ccaacatgct ggcagtgtat gatagtcaat
      421 gcggattcct gaagatgctc gtcaatcgct tcttcttctt cttctctttt taatgaaagc
      481 cacttttaca actaactata cttttactcc cttggccgta tttattgtac ttagcatagg
      541 tatgctgcca tggaaaacaa aatcgaatcc gctatggaaa tagaagaagc tggaaattat
      601 ctgcagctca gcttcagtcg agcaccgaaa aagaatcagg aggctctagc acagcttgga
      661 aagcgatggg tgcaatggct caacaaacat ggggtaagct ccaagatcta ctatctcaac
      721 agcagcacaa caacaaacga gcaagttcct gaaggcgtag aaagcattgc aaagattctt
      781 tctgtcagtg atgatgagat gcttggcgtg tctctgcaat tctatagaga ccaagctcac
      841 gcaaatgaag tctattcaaa gatgatgcaa gacgaaactt gtggcgcaat tggcaaggaa
      901 tttgatggtc tagtcaccca aggaaaaggc atgatcacag atggattcag tcgccttagg
      961 gtataggaat catagcaact tcagttggcg gtagtattgc tggtgatcct cttcccttct
     1021 ttttcttctt tcttttttag gccttcaact ttgacttcta tctgttcaac tcctgagatg
     1081 cattcagtta gctgttcgtg attgtaacgt agtatagatt agaaaatgga atacaagatt
     1141 tagagattta gagaagaatc ctagtacacg catatatcta tcttggtaca actccaaaga
     1201 ttgattgtcc atcaattata cttaggatgt ccagttgtcc tgtatctgtt atagttgttt
     1261 catcaatatc aatatcctct atggcaagaa cttgacccct aattgatact gtcattggaa
     1321 cattatctat gataggtgtg ttgccattga cagagatacg agtcatcaaa ttagagctaa
     1381 caggaggaat ggatccagtt gctgcggtgg tagacattcc atttccactc tccgtcagtt
     1441 caaaccgatg aggtgtagtg tcttcaatgg taatattgtg gattgctgtt ccattggttg
     1501 ctgctagatc catttcggct acgaaccgat taaccagccc ttcgtctaca aatattctcc
     1561 atcgcccagc caaaatataa ctactattct cagtaggaat atctccaccc ggtgcaggca
     1621 gactgctgcc tataattcca cgcagtagaa acgtcgttgt ggttgatggc agagtagctt
     1681 ctacttcccc tcctccgtct tcctgtcccc cgtcgagagg aggaagatcc aattgtccaa
     1741 aggcttcttg tataattgtt cctccaaagc caaggctagc tggcaataat aagagaacac
     1801 ccaagctcat agcagcaatc gctgcgttaa atttatctcc taaaaaagga aactgctctg
     1861 tcatcgtatt acaattgtag aattgcttat tttgatatat accttactgg tagcacgagc
     1921 tgctgcgtct atctaagtta acagacaaag aagtatgcaa atcatgcaat atcgacatat
     1981 cttcactaat tacaggagct atactaggaa gtgctgtact ttatgatg
//
LOCUS       DASQTF010000178         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_977486,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000178 DASQTF010000000
VERSION     DASQTF010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977486"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /gene="dnaG"
                     /locus_tag="VE308_04230"
     CDS             complement(<1..1228)
                     /gene="dnaG"
                     /locus_tag="VE308_04230"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF003108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HZB00214.1"
                     /translation="MVEKADIIGAIFGQTEGLLGPEMNLNELQKVSKVGRIEVNVETR
                     GNMTKGDALIPMSTDISTAALIAAAIESIDKVGPFPAKFGLVGIDDIRAIKKKVIVDR
                     AKKIVQDWATRTISEGDEMLKDVYDASKPGKLTSFGKAQLACGTGVFDSAWIILVEGR
                     ADVVNLLRAGYDNAIAIEGARIDESVTKLTEGKRVVAFLDGDRAGDLILKELHGVVKI
                     DKVLRAPQGKEVEECTPLEISEILKDVFLPEVQSVQQPIYPPHQPPHHQQQHHYERRN
                     YRRERDPQEDYRAPQVRQQEALQSAQPSKEALVEDSTEIVSAVRDVYPQINETLEAVV
                     LDDSMNTLLKVPVSEVIKRLNSAEGAKLLVIDGIVTQRLVDAADKAGIEYIVGHRMTD
                     LKKPADIRIRTFGDIGI"
     gene            complement(1371..1814)
                     /locus_tag="VE308_04235"
     CDS             complement(1371..1814)
                     /locus_tag="VE308_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZB00215.1"
                     /translation="MGYHYKVDDYETVERLRSFICMLNNESEKGSVIVVEGRRDAEAL
                     SRVGFTGNLAVFHHFKGIADFVDSHDSIGKKIILLLDMDRTGKYLTSRLVSQLQLSRH
                     SVNLSYKRTLARITNGKVRHVEDLVIYAPHMSGITGSRKDLYFYT"
     gene            1895..2581
                     /locus_tag="VE308_04240"
     CDS             1895..2581
                     /locus_tag="VE308_04240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZB00216.1"
                     /translation="MPNILSVQNIRCETLGLLEQMFVHDGYHIEKVNMQNDPVPTSPL
                     GYDAIVILGGPMAVYDNLPYLQKEQELIRNAMRNDTPVLGICLGSQLIAQAAGGLVYK
                     GRKKEIGWHNVYLTPASSNDIFRGFTDKMIRIFQWHGDTYDLPANAKILAYSDLYPQA
                     FRIGSAVGIQFHFEVDEPLIQTWMMEYSADLIAEKIKPESILPAPCELEELEARCRHI
                     YNNFARVLQK"
     gene            2653..>3080
                     /locus_tag="VE308_04245"
     CDS             2653..>3080
                     /locus_tag="VE308_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZB00217.1"
                     /translation="MTNEKVQKLFDDVYSNREKVITEELAKQVLSEYGVMVPKYALVK
                     SAEEAGIEAEKLGFPLVAKIVSPEILHKTDVQGVKVGLQNKADVKDTFNDMYGRLSKQ
                     YSVKGVLLEKMASSGGVELIVGLQNDPQFGPVIMAGIGGI"
BASE COUNT          802 a          739 c          676 g          863 t
ORIGIN      
        1 ttataccaat gtcaccaaat gtcctgattc ggatatctgc tggctttttc aagtctgtca
       61 ttcgatgtcc tacaatgtac tcgatacctg ccttatcagc tgcatcgacg agcctctggg
      121 tgacaatgcc gtctattaca agcagcttgg ccccctctgc gctattaagc cttttgataa
      181 cttccgacac cgggacctta agcaaagtat tcattgagtc gtctagcact acagcctcaa
      241 gcgtttcatt tatttgcggg taaacatccc gaactgcaga gactatttct gtactatcct
      301 ccaccagcgc ctctttggat ggttgcgccg actgtagtgc ttcttgctgt cgcacttgtg
      361 gagcgcggta atcctcttgt ggatcccttt ctctcctata attcctcctt tcgtaatggt
      421 gctgctgttg atgatgaggg ggctggtgag gaggatatat cggttgttga acagattgta
      481 cttcaggcaa gaatacgtcc ttcaaaattt cggatatctc aagcggggtg cattcttcca
      541 cttccttacc ctgcggagcc ctaagcacct tgtctatctt gacaactcca tgcagctcct
      601 tcagtataag atctccagct cgatcgccgt caagaaatgc cactacacgc ttgccttctg
      661 tgagttttgt cacagattca tcgattcttg caccttctat tgcaattgcg ttgtcataac
      721 ctgcgcgcag aagattgacg acgtctgctc ttccttccac caatattatc caggcagagt
      781 caaagactcc cgtgccgcag gccagctgtg cttttccaaa gcttgtgagc ttgcccggct
      841 tgcttgcgtc ataaacatcc ttgagcatct cgtctccttc gcttatagtt ctcgtggccc
      901 aatcctgcac tatcttttta gcgcggtcaa ctatgacctt cttctttatg gctcgaatgt
      961 catcgattcc aactagaccg aacttagccg gaaacggccc caccttgtca atgctctcta
     1021 tagctgctgc aattagcgcg gcggtagaga tgtccgtact cataggaatc agggcatcac
     1081 ccttagtcat gttgccccta gtttccacgt ttacttcaat tcttccaacc ttggatactt
     1141 tttgtaattc attgaggttc atttccggtc cgaggaggcc ctctgtctgc ccaaaaatag
     1201 caccaataat atcggctttc tcgaccaaac catcaacatc aaattttaac ttaacatgat
     1261 attttactat acctgtggtg ggcaatttct ttcataacct cgcagtttgc tggtatatag
     1321 ctttcacttt tctttgcttg aatataagtg ctttgtaaac cttcaggaaa tcatgtataa
     1381 aaataaaggt ccttgcgact tcctgtgatg cctgacatgt gcggtgcata gatgacaaga
     1441 tcctctacat gtctcacctt gccatttgtt atccttgcaa gtgtcctctt atatgacaag
     1501 ttcacgctat gcctgctcaa ctggagctgg gatactagcc ggcttgtgag gtacttgccg
     1561 gttctgtcca tatctaagag caggattatc tttttgccta tgctatcatg gctgtctaca
     1621 aagtcggcaa tccccttaaa atgatggaag actgcaaggt tgccagtaaa acctacccta
     1681 gaaagtgcct cggcatcccg tctaccttcc acgacgatga cagaaccttt ctccgattca
     1741 ttattgagca tgcagataaa actacgcagc cgttcaacgg tctcatagtc atcgacttta
     1801 tagtggtagc ccacggccca gctaatctgt agcgcctatc agatttaata tttttcctta
     1861 tggcataaat agaaatgagc gcacatgcta gatattgccc aatatacttt cagtacagaa
     1921 tattcggtgc gagacacttg gcttacttga acagatgttt gtgcatgatg gctatcacat
     1981 cgaaaaggtg aatatgcaga atgaccctgt gcctaccagc cccttgggat atgacgccat
     2041 agtaatcctc ggaggcccga tggcagtcta tgacaactta ccctacctgc aaaaagagca
     2101 agagcttatc aggaatgcaa tgagaaatga cacacccgta ttgggaatct gccttggctc
     2161 acagctcatt gcacaggccg caggagggct cgtctacaag ggtaggaaga aagagatagg
     2221 atggcacaat gtatacctga cgccggctag tagcaatgac atctttagag gcttcaccga
     2281 taagatgatc aggatattcc agtggcatgg tgatacatat gatctgccag caaacgcaaa
     2341 gatccttgcg tattccgatc tatatccgca ggcatttaga atcgggtctg cagtaggcat
     2401 ccagtttcac tttgaagtcg atgaacctct catacaaaca tggatgatgg agtacagtgc
     2461 cgacttgatc gctgaaaaga tcaagccgga gagcatactg cctgcacctt gtgagttgga
     2521 agagctagag gctcggtgca ggcacatata caataatttt gcaagggtct tgcagaaatg
     2581 aataaaaata atacatttca cataataggt aaaactatat aaaagctaaa caggtttttt
     2641 cactcaataa aaatgaccaa tgagaaggtt cagaagctct ttgatgacgt ttattcaaac
     2701 agggaaaaag taatcactga agagctcgcc aaacaagtct tgagcgagta cggcgtcatg
     2761 gtaccgaaat acgcgctggt caagagcgct gaagaggcag gaatagaagc agagaaatta
     2821 ggttttcccc tcgtcgcaaa gattgtttca ccggagatct tgcacaagac agatgttcaa
     2881 ggtgttaagg tcggccttca aaacaaggct gatgttaagg atacatttaa cgatatgtat
     2941 ggcaggctta gcaagcagta cagcgtaaaa ggcgtgctgc tcgagaaaat ggcctcaagt
     3001 ggtggcgtgg agctcattgt tggtctgcag aatgacccgc agtttggccc tgtcatcatg
     3061 gccggcatag gtggtattta
//
LOCUS       DASQTF010000179         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_978155,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000179 DASQTF010000000
VERSION     DASQTF010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978155"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            489..896
                     /locus_tag="VE308_04250"
     CDS             489..896
                     /locus_tag="VE308_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6e family ribosomal protein"
                     /protein_id="HZB00218.1"
                     /translation="MAQFKLIVSDRNGRTIAQELKDRTAQPLLGTRVGSIIDSSIVGV
                     AGGKLKITGGSDKSGTPMRPDVHGGVRKYVLLSTGVGNRSEARVRKLVRGNMVTEEIY
                     QLNSMLIEGILPEKREEAVESSGTETEPSNKKQ"
     gene            939..2201
                     /locus_tag="VE308_04255"
     CDS             939..2201
                     /locus_tag="VE308_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HZB00219.1"
                     /translation="MHWKETLPDWYIKEYGYQPSVNIGTSGHVDHGKTTLVEAITGVW
                     TSAHSEELRRGITIKIGYADAAFYKCPQCPPPVCYSTQPQCTNCGGKSELLRVVSFVD
                     SPGHESLMANMLSGAALMDGAILVMAANEKVPQPQTREHLLALSVLGIQQIVLVQNKA
                     DLAEYEEALDNYRQIKDFVSGSIAEKAPIIPVSAQHKINIDALIEAIEKTIKTPARIK
                     NAEPVMHVLRSFDVNKPGSPIKQVKGGIIGGALVQGQLNVADEIEIRPGVLDEKKGKY
                     DPIASQVATLGTGAGLVKSVRPGGLVAIGTKLDPTFTKSDSLIGSVVGKPGTLPNDVE
                     DIMIDVVLFDTAVGTAEMVKVEPLKPKESVRLNIGTAAASGYVTNVRDSKIEVKLKKP
                     VCLMPRSRVAISRRIAERWRLIGSGITA"
     gene            2205..2609
                     /locus_tag="VE308_04260"
     CDS             2205..2609
                     /locus_tag="VE308_04260"
                     /inference="COORDINATES: protein motif:HMM:NF037434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00220.1"
                     /translation="MQVVCDTSFLMVLVSKPIKRVAKIESELGRLDFLVPDIVEGELK
                     RLAQNAGPKRSRLAKTALELARAKFNTVAVAPAKHVDDSIIDYAMAQKCAVATIDTNL
                     RRRLIANQVLVLSLSNDNLIIANPKIDTKDHL"
     gene            2650..>3119
                     /locus_tag="VE308_04265"
     CDS             2650..>3119
                     /locus_tag="VE308_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZB00221.1"
                     /translation="MLDFDGIKFHWLGHDGYKLTDEDKANIYIDPYKISESHHNKNDA
                     DIILISHNHFDHLSVDDLKHLIGNNTTIVAAKECIEQLKNVRVAETKGVLPGDMLTVQ
                     GIPIETTAAYNTNKHFHPKADGKVGFIITLNKMRIYHTGDTDDIPEMNGTDPDVA"
BASE COUNT          961 a          666 c          733 g          759 t
ORIGIN      
        1 catatgatga ttacattgaa ataatgaagg gcaaggcggt tgatctttca gacaactttc
       61 agatgagctg ggacagaaac cctttttgac taccatgtga gacccttctc actgcagtcc
      121 atatgtccac cacaatttgg acaaatcata tggcaggcct gcatatgctt catttcaaac
      181 ccgcagcgag ggcaatcaat agcttcttgg tcttctccca accatagtca attctcagca
      241 ataactttta actgttttac ccatcggacc ctaattgctg tctgtatatt atatagcgag
      301 caagtcaata tccaaatgct cttgctcgtg ttttaatatt caaacctaag caattgatat
      361 ttcgctaact gcgaatatgc tctttcatcg gaaaaactac atctgcatat atctacacca
      421 cgtgtacgag aagagggagg acttttggcc aaagattaat taatcgtgta tatgcctaat
      481 cttgtagttt ggctcagttc aaactgatag tttcagacag aaatggtaga accattgcac
      541 aagagctcaa agataggaca gcccaaccac ttttggggac aagagtaggt agcatcattg
      601 actcttctat tgttggagta gctgggggta agctaaagat aaccggcggc agtgacaagt
      661 ctggcactcc aatgcggccc gatgtccacg gcggagtcag aaagtatgtc ttgctttcaa
      721 caggtgttgg gaacaggagc gaagctagag ttagaaagct ggtaagaggg aatatggtga
      781 cagaagaaat ttatcagctc aactcgatgc taattgaggg tatattgccg gaaaaacgag
      841 aagaagctgt cgaaagcagc ggaacagaaa cagagccatc aaataaaaag caatagttcc
      901 gtaccttaat atctccggga attttacgat actaattgtt gcattggaaa gagaccctgc
      961 cagattggta tatcaaagaa tatggctacc agccatccgt taacattggc acatcaggtc
     1021 atgttgacca tggcaagacg actcttgtcg aagccatcac aggagtctgg accagcgctc
     1081 atagcgaaga gcttagaaga ggcatcacca ttaagatcgg ctatgccgat gctgcatttt
     1141 acaaatgtcc tcaatgcccc ccgccagtat gttatagtac tcaaccccaa tgcactaact
     1201 gcggaggaaa aagtgaactt ctcagggtag taagttttgt cgactcccca ggccacgaaa
     1261 gcttaatggc aaatatgctc tcgggtgcag ctttgatgga tggagcaatt ttggttatgg
     1321 cggctaatga aaaggtgccg cagcctcaga caagggagca cttacttgca ctctcagtcc
     1381 ttggcataca gcagatcgtt cttgtacaga acaaagcaga ccttgctgaa tacgaagaag
     1441 cacttgacaa ttataggcag atcaaagact tcgtcagtgg aagtattgca gaaaaggcac
     1501 caataatccc agtgtcagcc cagcataaga tcaacattga tgcgctcata gaagccatcg
     1561 aaaaaactat caagacccca gcacgtataa agaatgccga accggtaatg cacgtgttac
     1621 gttcatttga tgttaacaag cctggaagcc caatcaagca ggtcaaagga ggcatcattg
     1681 gcggcgcgct agtgcagggt cagttaaatg ttgcagatga gattgagatc cgtccaggag
     1741 tcttggatga aaaaaagggc aagtatgatc cgattgcttc tcaagtagca acgcttggta
     1801 ctggtgcagg tcttgtcaag tcagtcaggc caggagggct tgtggcaata ggaacgaaac
     1861 ttgatcccac atttacaaaa agcgactcac tcattggctc tgtggtgggt aaaccgggga
     1921 cgctgccaaa tgatgtagaa gatattatga tagatgtggt gctttttgat actgcagttg
     1981 gcactgcgga gatggtcaaa gtcgaacctc ttaagccaaa agagtcagtc agactcaaca
     2041 tcggcacagc tgcagccagt ggctacgtta caaatgtgag ggacagcaag attgaagtaa
     2101 agctgaaaaa gccagtgtgt ttgatgccca ggagcagagt cgctatcagc aggagaatcg
     2161 ctgaaagatg gcgtctgatt ggctcaggaa taaccgcata gtatatgcaa gttgtttgtg
     2221 acacgagttt tttgatggtc cttgtttcaa agcccataaa gcgggttgca aagatcgagt
     2281 cagagcttgg cagacttgac ttccttgtcc ctgacattgt agaaggagag ctaaaacggc
     2341 tggctcaaaa tgcaggtccc aagcggtctc gccttgcaaa gacagcgctt gaacttgcga
     2401 gagcaaagtt caacacagta gctgttgcac ctgcaaagca cgtggacgac tcgataatcg
     2461 attatgcaat ggctcaaaag tgtgcagtag caacgataga caccaacctg cgccgtaggc
     2521 tcatagcaaa tcaagttctg gtactgagcc tcagcaatga taatctaatt atcgcaaatc
     2581 caaaaatcga tacaaaggat cacctttaaa taaaattctt ggcgccaaga tacaagtagt
     2641 ggtaatagta tgctagactt tgatggaatc aagtttcatt ggcttggcca tgatggctac
     2701 aaactgacgg atgaggacaa ggctaatatt tacatagatc cttacaagat ttccgagtcc
     2761 catcataaca aaaatgatgc tgacattatc cttatctctc acaatcattt tgaccatctg
     2821 agtgtggacg acctcaagca cctcataggc aataatacca cgatcgtagc cgccaaggag
     2881 tgtattgagc agttaaagaa tgtcagggtg gcagaaacga aaggagttct tcctggcgat
     2941 atgctcactg ttcaaggtat cccaatagag acgactgcag cgtacaatac caacaagcat
     3001 ttccacccaa aagcagatgg aaaggtcggt tttatcatca ctttaaacaa gatgcgtata
     3061 taccatactg gtgatactga tgatattcca gaaatgaatg ggaccgatcc agacgtagc
//
LOCUS       DASQTF010000180         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_978216,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000180 DASQTF010000000
VERSION     DASQTF010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978216"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1491..1757)
                     /locus_tag="VE308_04270"
     CDS             complement(1491..1757)
                     /locus_tag="VE308_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00222.1"
                     /translation="MFGSSLSDSDTNFNDIAIFKLGCDIGKPSWFIDCCRHFYAILLD
                     RKNDVTAFHCIWLATNLASDIPCCVCLDIGNPDLILQVHIRPLV"
     gene            complement(2056..2160)
                     /locus_tag="VE308_04275"
     tRNA            complement(join(2056..2076,2107..2160))
                     /locus_tag="VE308_04275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2123..2125),aa:Met,seq:cat)
BASE COUNT          811 a          553 c          600 g          719 t
ORIGIN      
        1 tccaagatgc ttctccctca attcgtcagg aatagtctta ccaccaattt ctttctcaag
       61 ttggtttatt attacgtctg ctgctttttt ggcagcttct tcaggcgttg ttgctttaac
      121 ctcttctttt tccttttcct ctacttccgc catcccaatt agttccacaa agagggaagc
      181 tataaatgtt cttgtattag agaatcaagc agagaactta gcagctttct tgcttagtta
      241 gttggtcaaa tagtaacaaa ctgttttgag gcttcttctt acatttagta tttggttatt
      301 ttttagcatt agttattcaa tcgtattatt ctaagattgg agtggtggat gctatatacc
      361 ctgataataa tcatataata attctcatca tcatcatctt caccgttttc tctttctagt
      421 tctttttctc gtgagatgag gatgataaaa acagagccct tcggttaccg ccagcgccag
      481 aagaagaaga tgaggatgag gatgattgag gatatgatga cttgttgtta ttgttggcaa
      541 atagattccc aaacacctct aggattactg atcgcggggc ttcctgcttg agctttagca
      601 atctaaattt acttcaatgg cacaatgcct tggtttgaac ctaatgtctg gccgaatgtc
      661 tagatcctag ctcatagatt atagatagaa gctacacagc ttttgaatta tacgactata
      721 tagctactgc atcaacaata gataacatca tgcacacgct ccacgttgca tttgacgaca
      781 cggactcgcg tgagggcaga tgcacaacac accttgcatt caaggtagta gagcatctga
      841 ggaaaataca gggcgcacag cttgtcgatt atccattgtt gattaggctt aatcccaata
      901 tcccttggaa aactaggggc aatggtgcag tctgcctgag gttaaaagtg caagatgtag
      961 acagagtcat agactatgtc aggcaggcag tggaagaagg ttccgccatt ggcagcggag
     1021 ctaaccctgc aattgcattc cttgaaagcg actatgtgcc cgcaaagctt cgagagttta
     1081 gcgcgctcgc aatgtgcgat ataatgagca gacaggtgtc agaaaaggta gctaaagcct
     1141 ctggtgttca atactttacc tttggcaatg gacagggact tgttggctct cttggtgcaa
     1201 tgggttgtct gctggaggat gatcatacct ttgagctaat tgcatacagg aagcagaaga
     1261 actggggcaa gctgcgtgta atagataagc agagtgtcat taagtttagc atagacacct
     1321 atccccacac tttcaacaat tacgatcgaa accacgggcg tgtgttgatt gcaccgcata
     1381 gtccagaccc agtcttcttt ggtataagag gcgagagccc agatatagtt gagtcggcac
     1441 tccaggcatt gcatccagaa gaagagctag gtggctggac agtttttcgc tcaaaccaag
     1501 ggacgaatat gcacctgcaa aatgagatca ggattgccaa tgtcaaggca tacacagcag
     1561 ggtatatcag atgcaaggtt agtagcaagc catatgcaat ggaaggcggt cacgtcattt
     1621 ttacggtcaa gcaggatggc gtagaaatgc cggcagcagt ctatgaacca actgggcttg
     1681 ccaatatcgc aaccaagctt gaagatggcg atgtcattga aattggtgtc ggagtcagaa
     1741 agggaactac caaacacccc aaaattctaa atgttgaata tttgtgggtg ttcaagcttg
     1801 cgcctgttta tgaaatattg aacccattgt gcaaattgtg tggcaagcgt atgaagtcag
     1861 agggcaaaaa taagggctat cagtgcgaca ggtgcaaaca caaagaccac aacacaaaaa
     1921 agattctggt acctcaggag cgtagaatca aaactggatt atacatccca acacccaaag
     1981 cacacaggca tctgacaaag ccgttacaga ggtacggcat tgaaaagaca aaattcctgt
     2041 ttgaaaaaaa ggtagtagcg aggggttgat ttgaacttac ggccacctgc aatttgcaaa
     2101 agtggaaacc gctctgcggg ttatgagccc gccgggataa cctggcttcc ccacctcgct
     2161 gacagcatct tgacacaagg ggaatatatc tctgtctgca ctgcataatc gagaaggaat
     2221 acataacagc gacgaacatt gttacatcct ttccgttttg tcaaaagaca cataccggct
     2281 tcattcaaga tcgggttgac agactcagag cagatgttgc ttaatattct ctgcaacaat
     2341 tcttgatttt gaaatgaccg ttgaaggcgc gcatacatat tttacctaaa ggagacgagg
     2401 tcgcatttcg cttatctttt tggtgtatcc ataaggagaa tggatgtaca caacctacat
     2461 aggcaagtac aatgcccaca taagcgaaat ccgattccta cgcatagcaa aaaggtaagt
     2521 aaatgagaga gagaggataa accggaaatc ttgatacgat ataacagatg gatataattc
     2581 aagtactaaa gcaaaaaaag agaaaagaca aatagataga tatcactccc atgcatcagc
     2641 aatttgtcaa tccttaataa tagcagcaaa caattgcctt gtt
//
LOCUS       DASQTF010000181         3344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_879851,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000181 DASQTF010000000
VERSION     DASQTF010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879851"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..440)
                     /locus_tag="VE308_04280"
     CDS             complement(9..440)
                     /locus_tag="VE308_04280"
                     /inference="COORDINATES: protein motif:HMM:NF001617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HZB00223.1"
                     /translation="MLAGGYRYLDHMTDAVIEAYGTTLEEAFENAAKALCDTMIDLKS
                     VRPKREIKFSAKGDDLYSLLFDWLDKVMLLLVSDGIAMSQFSVKIQQANDNYLLEGTA
                     RGEPLELDRHHYKVEIKAVTYHEMEIRQEKGIITARFLLDL"
     gene            521..1804
                     /gene="hisS"
                     /locus_tag="VE308_04285"
     CDS             521..1804
                     /gene="hisS"
                     /locus_tag="VE308_04285"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZB00224.1"
                     /translation="MARLELPRGMRDLDPDEYSNINYVRDRFFEIARLFNFQLTEPSP
                     LEMLATLEAKSGAAISNEIYAFKDKGGRDVALRFDLTIGLTRYVESRRDLKMPVKLAS
                     FAGVWRYDEPQAARYRYFHQWDIEAYGPFSQESDAEVIEFVYRFFKTLGLKVAIEIND
                     RQLLEQYIRKKLGISSEETMLEMFRAVDKVPKKGAQAVLAEYKDKIQPAKMQELIELS
                     KLKGNVDEVARKADMTGLDSWRQLVSLMDLLNAKKVEEARINLGIVRGLDYYSGMVFE
                     AFDQATDSGALVGGGRYNRLTEAFGRKDIGATGVAGGVERIVMALKRHGILKQPPRPL
                     VHVANATEDLRAKALELVSSLRTGGIAVDYDMLGRALRKQLEYASNRGASFVVIVAPN
                     EIATGQVILRSMKDGTESKHYADNLKETLDKMLRV"
     gene            complement(1794..2078)
                     /locus_tag="VE308_04290"
     CDS             complement(1794..2078)
                     /locus_tag="VE308_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00225.1"
                     /translation="MESTNLRDYLANAPDAKAFLAPNSSDEELVTRAKERLTENGIRQ
                     DRIEINYDVQQLKTGDIYISYDPPDLIVRVVYEKKPNGMVKMKSVATIKL"
     gene            2942..>3344
                     /locus_tag="VE308_04295"
     CDS             2942..>3344
                     /locus_tag="VE308_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZB00226.1"
                     /translation="MADITTLAHDVLIIGSGLAGLRAAISAAAVDRKLTIAVISKVQV
                     MRSHSVSAEGGTAAVLYEEEGDNQEAHIYDTIKGSDFLADQDVTELLVKSMPYEVYQL
                     DHWGMPWSRRKDGRIAQRKFGGYSFPRATFAQ"
BASE COUNT          935 a          712 c          747 g          950 t
ORIGIN      
        1 ctgcatggtt agaggtcaag caggaacctt gcggtgataa tacccttttc ttgcctgatc
       61 tccatttcat gatatgttac tgccttgatt tccactttgt agtggtgccg gtccaattca
      121 agcggctctc ctcttgcagt cccttcaagc aaatagttgt cgttagcctg ttgtatcttt
      181 acagaaaatt gagacattgc tatcccgtct gacaccagca aaagcatcac cttgtctagc
      241 cagtcgaaga gcaacgaata aagatcatcg cctttggcag aaaatttaat ctctctttta
      301 ggtctgacac ttttcaagtc gatcattgtg tcgcatagag ccttggcagc gttttcaaag
      361 gcttcctcta aagtggtccc gtaagcctct ataacggcat ctgtcatatg atcaaggtat
      421 cggtagccac ctgccaacat aacgatgaac aaatgacgac tatttattcc acgccaaaat
      481 atcgcaaaat ctaaatgtta acgcaccgct ggctactggt atggccaggt tagagcttcc
      541 ccgtggcatg agggatcttg atcctgacga gtatagtaac ataaattatg tccgcgatag
      601 gttctttgaa attgctcgac tgttcaactt ccaactcaca gagccatctc ctcttgaaat
      661 gcttgctaca ctcgaagcaa agagcggtgc tgcaatttca aacgagatct acgctttcaa
      721 ggacaaggga ggcagagatg tagcccttcg ttttgacctt actatcggtc taacgcgata
      781 tgtcgagtcg cggcgcgatc tgaaaatgcc agtcaagctg gcttcatttg ctggcgtgtg
      841 gcggtacgac gagcctcaag ccgcgcgcta ccgctacttc catcagtggg atatcgaagc
      901 atatggtcca tttagccaag aatcagatgc tgaagtaatt gaatttgttt acaggttttt
      961 caaaacattg ggcctaaagg tagcaataga gataaacgac aggcagcttc tcgagcagta
     1021 catcagaaag aaacttggta tttctagcga agagacgatg cttgaaatgt ttcgtgcagt
     1081 agacaaggta ccaaaaaagg gggcacaggc cgtgcttgca gaatacaagg acaaaataca
     1141 gcctgcaaaa atgcaggaac tgatagaact gtcaaaacta aagggtaatg tagacgaagt
     1201 tgcccgcaag gctgacatga cagggcttga cagctggcgg caactagtca gcttgatgga
     1261 cttgctcaac gcaaagaagg tagaagaagc gcgcattaac cttggcatag tgcgtgggct
     1321 tgactactat tctggtatgg tttttgaagc atttgaccaa gccactgatt ctggggcact
     1381 cgttggtggg gggaggtata acagactcac tgaagcgttc ggaagaaaag atattggagc
     1441 cacaggcgtt gctggagggg ttgagcgaat agtgatggcc cttaagagac atggtatatt
     1501 gaaacaacct ccaaggccac ttgtacatgt cgcaaatgca acagaggatc tccgagcaaa
     1561 ggcgcttgag ctggtttcat ctctgcgcac aggtggtata gcagtcgatt acgatatgct
     1621 tggaagggcg ctcagaaagc agttagagta cgcttctaat agaggtgcat cgtttgtagt
     1681 aattgtagca ccaaatgaaa ttgccacagg tcaagtaatc ctgagatcta tgaaggatgg
     1741 aactgaaagc aaacactatg cagacaattt gaaagaaaca ctcgataaga tgcttagagt
     1801 ttgattgtgg ccacactctt cattttgacc atgccgtttg gttttttttc gtataccact
     1861 ctgactataa ggtctggtgg atcatagctt atgtaaatgt cgccagtctt cagctgctgt
     1921 acatcgtagt taatttcaat tctgtcctga cgaatcccat tctctgtaag tctttctttt
     1981 gcccgggtca cgagctcttc atcggaagag tttggagcca gaaatgcttt ggcatctgga
     2041 gcatttgcca aataatccct caagtttgtg ctttccaata ataagagcca gccaggtaat
     2101 tactattatt tatgttttca gcaccttacc agaatattga tatgactgca gtttacctgc
     2161 atatcccttt attagctgca attagatata ctcatctttt caacaatttg ttaagccctg
     2221 gcaattttct tttgcccgag aataataaat cgacctctga tagaatctta taccaactca
     2281 cctggcaaaa aagcgcgtcg atatcgccag ctccgtccaa ctcctaaata cttttggcaa
     2341 tgcccttatc aattggatgc tatgctaaaa aacatggaca aaataaaatt gcagcattta
     2401 catagaggat tctttttgaa ttatgactag gcataaagat ctgtgattgc gtaaggttct
     2461 tgtctagacc attctttgtc atgactagtg tacaacttat tcaaatcgag actttgaata
     2521 tgctgcaatg taaacatttc aatcaaaatg tctcaacaag tcccctgaca tagctcttag
     2581 tctatctgtc atatagctca ggtttactat gtgtcatttg gaaggagaat ggcttccctc
     2641 ttcttgtcct cctttgcaga tacactgtga cgacgataac tatcaggtaa aaatgtttag
     2701 agcacttatc atttaatgct cccatgtatt gtactttatt tcaaactttt ctatagatga
     2761 tcctttcttt gtatttttgt actactacgc tatcaagaat atcatgaatc atatttagta
     2821 ttacggctgc atgtatacaa ctgttaatta cttttagttc tgttgcaatt gattgcagga
     2881 gtttaaggta tatatgagaa ttagctgcct tcctgcatgg aaggaaagag ggaccgcata
     2941 gttggctgat attactactc ttgcacatga tgtactcata atcgggtcag ggctggcagg
     3001 cctccgtgca gctatttctg cagctgccgt tgacagaaag ctgaccatag ctgtcatttc
     3061 caaggtgcag gtaatgcgct cccattctgt gtctgcagaa ggtggcactg cagcagtgct
     3121 ctatgaagaa gagggcgaca accaagaagc tcatatttac gatacgatca aggggagtga
     3181 cttcttagca gaccaagatg taactgagct gttagtaaag agcatgcctt atgaagtcta
     3241 ccaattagac cattggggaa tgccatggtc caggcgcaag gatggtagaa tagcacagcg
     3301 caaatttggt ggctattcat tcccgcgcgc cacatttgca cagg
//
LOCUS       DASQTF010000182         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_64413,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000182 DASQTF010000000
VERSION     DASQTF010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64413"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1045
                     /locus_tag="VE308_04300"
     CDS             11..1045
                     /locus_tag="VE308_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZB00227.1"
                     /translation="MNFVVTILRMKHPDLPMMKLPLFTWSILSTSLIILMSMPTFAAA
                     LIMLYTDRLGVSGFFNPAMGGDPLSYQHLFWFTFHPEVYVFVIPAIGMIYEIIPKFSR
                     KPIFSYTSGITAFILLTIVSFASWAHHMFATGMSFTEKTVFMIGTLAAVPASAMHIFN
                     WIATMWGGRIRFRAPMAFVVGGIITFFLAGAGGVVNSAMPLDFLTHDSYWVVGHMHLF
                     LMGIITFAFVGFIYYLFPLITGRMYNERAAMLQFWLMFIGVFLIFVTQHLLGLYGMPR
                     RVADYVPVEPLIVMNQISSVGAYIMAVAMGIFLFNLIKSAGWGKPANMEDPFGIREKY
                     YDYRRREPHH"
     gene            1150..2010
                     /locus_tag="VE308_04305"
     CDS             1150..2010
                     /locus_tag="VE308_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZB00228.1"
                     /translation="MIVTIAIGAAILIPFFSQMAEVPPPVTQIRTETPAAEEEGEEQA
                     PAEPGTTAIAILQGAAVQGSPDYDPDAAQVPVGNTIVWDNQDTVPHTATSGTGPQDPN
                     SAQLFDTGIVNGGEESTAVELQGVSEGQTIPYYCIVHPYMTSELTVAAAEQGGGQTQG
                     GGAAQQQGGGANQTQGETTGGAGGAAAGGPTINILEGSSIQGSPDYDPEELTASAGAE
                     VSVVNQDTLPHTVTSGTGPQDPNSAQLFDTSLINGGESATLSLAQVTAGQYDYYCMVH
                     PYMTGKVVVQ"
     gene            2293..2838
                     /locus_tag="VE308_04310"
     CDS             2293..2838
                     /locus_tag="VE308_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZB00229.1"
                     /translation="MRHARLYYGKMDAFECITTKLDLREFSSQDISAEIKSKILEAAR
                     LTGSGLNTQHWRFVVIEEKENLKKLAEDSTSGGWVSGANFAVIVLTNSRYKFHMIDAG
                     RVVQDMQLAAWNYGVASCLFTGVQEEKLRSDYGIPKDLNPTIIVGFGFSAKQISGKKR
                     KNRMSLDELVYYEKYGNPGRS"
     gene            3156..>3449
                     /locus_tag="VE308_04315"
     CDS             3156..>3449
                     /locus_tag="VE308_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZB00230.1"
                     /translation="MQANQQQQLDAVDKKLLNEIQWVFPLVDRPYLEIAKRYDISEED
                     VMRRIALMKNMGIIRQINAIFDTRRLGYKSALIAFAVRPDKLDYVAGKVNEHPG"
BASE COUNT         1026 a          724 c          809 g          890 t
ORIGIN      
        1 tctaggcgcc atcaacttcg tcgttactat cttgagaatg aagcatcctg atctgccgat
       61 gatgaagcta cctcttttca cttggtccat cctttctaca tctcttataa tcctcatgtc
      121 tatgccgacc tttgctgctg cactgataat gttgtatacg gacagacttg gagtttccgg
      181 tttctttaat cctgcaatgg gaggagatcc actatcatac caacatctct tttggtttac
      241 atttcatcct gaggtctatg tattcgtgat acctgcaatt ggtatgatat atgagataat
      301 tcccaagttt tcaagaaaac ctatattcag ttatacatcg gggattacgg catttatctt
      361 gctgacgata gtctcttttg cttcctgggc tcatcacatg tttgcaacag gtatgagctt
      421 tacagaaaaa acagtattca tgataggcac attggcagct gttccagctt cagctatgca
      481 cattttcaac tggatagcga caatgtgggg aggcagaatc cgattcagag caccaatggc
      541 atttgtggtt ggtgggatca taacattctt tctagctgga gctggcggtg tcgtaaacag
      601 tgcaatgcca cttgactttc taacacacga tagctactgg gtagtagggc atatgcatct
      661 atttctcatg ggcatcataa cttttgcatt tgtgggcttt atatactacc tattcccgct
      721 cataacgggc aggatgtaca acgaaagggc agcgatgctc cagttctggc tcatgtttat
      781 aggggtattt ttaatattcg ttacacagca cttgcttgga ctgtacggca tgcctcgtcg
      841 agtagcagat tatgtaccag ttgaaccgct tatcgtgatg aaccagatct cctcagtagg
      901 cgcgtacata atggctgttg ctatgggaat attcctcttt aacctgatta aaagtgcggg
      961 atggggcaaa cctgcaaata tggaagatcc atttggtatt agagagaagt attatgacta
     1021 tagacggcgt gagcctcacc actaaagaga gggagagaaa gagatacgag ttcgcatgac
     1081 tcacacagtg acgataaaga accgataatc ctcacatcgc ctagaagatt tggaaaaggg
     1141 cttgcaatag tgatagtcac aatagccata ggggcagcaa ttctaattcc attctttagc
     1201 caaatggctg aagtccctcc acctgtgacc caaatacgca cagagacccc ggctgcagag
     1261 gaggaggggg aggagcaagc tccagcagaa ccaggcacca ctgctatagc gatacttcaa
     1321 ggagcggcag tacaggggag ccctgactat gatccagacg cagctcaggt gcctgttgga
     1381 aacactatag tttgggacaa tcaagacaca gtcccgcaca ctgcaacctc tgggactggt
     1441 ccccaagatc caaactctgc acagttgttt gacacgggca ttgtcaacgg aggcgaagaa
     1501 tcaactgcag ttgagctaca gggtgtaagc gagggccaga ctattcccta ctattgcatt
     1561 gttcatccgt atatgacaag cgagcttacg gttgcagcag ctgaacaggg cggtggccaa
     1621 acacaaggag gaggagctgc tcaacaacag ggaggaggag ctaaccaaac acaaggagaa
     1681 acaacaggtg gcgctggtgg agctgccgct ggaggaccca caataaacat ccttgaaggc
     1741 tcttcaatac aaggcagccc cgactatgat ccagaggagc tgacagcaag tgctggtgct
     1801 gaggtcagcg tggtcaacca agacacactt ccccatactg taacctcagg aaccggaccc
     1861 caagatccaa actctgcaca gttatttgat acaagcttga ttaatggcgg tgaatcagcc
     1921 acgctgtctc ttgcacaagt cactgcgggt cagtacgact attattgcat ggtgcatcca
     1981 tatatgacag gcaaagtagt ggtacaataa gtaaaagaag ctagcccgca catcttgtga
     2041 atttagctga tagagggtta tcctgacaaa aagaatgacg aattgacgaa gagagtacaa
     2101 gaaatgccga tatagtccaa ttaacatctg aagagagaag aattacatcc tgctgcctct
     2161 cgtcccaagt aaagcaaggt agcgccggcg aaaattcagg tagttctagc ggtaatagca
     2221 gacctcaaaa catgtcaagt gagaaatagt ttccgacact gtgcctatat accgtatatt
     2281 tccatatcct ttatgagaca tgcacgcctt tattatggca aaatggatgc ttttgaatgc
     2341 ataacaacta agcttgatct tcgagaattt agctcacaag atatttctgc agagataaag
     2401 tcaaaaatct tggaagctgc acggcttaca ggtagtggac tcaacactca gcattggcga
     2461 tttgtcgtta tagaagaaaa agagaatcta aaaaaacttg cagaggatag cacaagtgga
     2521 ggttgggttt caggtgccaa ttttgcagta attgtgttga cgaactctcg ctacaaattt
     2581 catatgattg atgctggaag agtggttcag gatatgcaat tagctgcttg gaattacggt
     2641 gtagcatcat gtttattcac tggagtacaa gaagaaaaat tgcgcagtga ttatggcatt
     2701 ccaaaagatc taaatccaac gatcatagta ggttttggat tttctgccaa acagatttct
     2761 ggaaaaaaaa gaaagaaccg catgtcacta gatgaacttg tctactatga aaaatatggc
     2821 aaccctggga gatcataaga agtgtgttgt tctaaattta cattagaata ggataggctg
     2881 atggatttta tcggtattgc atgactgcag agagcagatt ccgtggggat agattaaaaa
     2941 catggagttt gtatcttcta caaataagca gttttaagaa agggaacagc tactttcata
     3001 ccgcagaaag atacctcgcc atgaagttat gtctcctggc aaggttatca ttcaagcgat
     3061 atccatgtac tgctatttgt ggtaaaaaca ccagtgcatt attaccagag gtaggttttt
     3121 aatggattga caacaacaac ttttactgcc cgagcatgca agcaaaccaa caacagcagc
     3181 ttgacgccgt cgacaaaaag cttctaaacg agatccagtg ggtctttcct cttgttgata
     3241 ggccttatct ggagattgca aagagatatg acatttcgga ggaagacgtc atgcgtagga
     3301 tagcactcat gaagaacatg ggcatcatcc gacagatcaa tgccattttt gatacaaggc
     3361 gacttggata caagagcgct cttattgcgt ttgcagtcag acctgacaaa cttgactatg
     3421 tggcagggaa agtcaatgag caccctggg
//
LOCUS       DASQTF010000183         4904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_708811,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000183 DASQTF010000000
VERSION     DASQTF010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708811"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VE308_04320"
     CDS             complement(<1..981)
                     /locus_tag="VE308_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HZB00231.1"
                     /translation="MVVPAIRMERGITAFANGMKNDENIKPSCPECDSPIIFDVYHGE
                     YICQSCGYVVLEKMDYCGHETHSNNSDDRLKNLRGSGQTSSLLMNYGLQTEISLSDKD
                     YNGKPINFANAERIACVRKLHARLKASSEERRIARVLSVIDKICSKLLLPRSLSETAA
                     KIYRSYSSKNNTKGMSVNGNAIAAVYLACKQSCIARGLEEIVDSTDFCSNKRSSLRIA
                     FRCYKRMTMCMTNAIEADKMKIPSGTFDHVIPLDKYIAKISNRARIDWRIQKLALEIA
                     RQTGDNILLSGKDPVGIASAYLYISACLCGYHMYLTDIANFSQVTEITIRT"
     gene            1347..1562
                     /locus_tag="VE308_04325"
     CDS             1347..1562
                     /locus_tag="VE308_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00232.1"
                     /translation="MSRSLAILIFWALGFTIGSAGYLMVPNAVSWLGNILPEIVVNQA
                     ILGSILAGVVGSAVSTFTVVTWANRAP"
     gene            1711..2052
                     /locus_tag="VE308_04330"
     CDS             1711..2052
                     /locus_tag="VE308_04330"
                     /inference="COORDINATES: protein motif:HMM:NF016798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HZB00233.1"
                     /translation="MAYDIGFMLTSVSSGGISGFLIGYAIKKGIKIILGIAGLLLGGL
                     AYLNYKGLVSVDWESIASMSNKAITEFSLSSANYASSVMDSQVVPTMMNFGVPLTGSF
                     AAGFILGCLKG"
     gene            complement(2872..4146)
                     /locus_tag="VE308_04335"
     CDS             complement(2872..4146)
                     /locus_tag="VE308_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HZB00234.1"
                     /translation="MKFLFVSLEALSVDLAYVIRQEGNDVLLYIQSLTERDVGDGFVD
                     KVDSWEDHADWADVVVFDDIGFGKIAEKLRAEGKKVIGGSLYTDKLENEREFGQQELT
                     KAGVSVLPSWNFSNFDDAIRFVHENSGRYVIKPSGKAQSEKELLFVGQEADGNDVINV
                     LAHYKKNWSNKIKAFQLQQYSSGVEVAVGGFFNGKEFIRPVNINFEHKRLFPGNIGPS
                     TGEMGTLMLWIQKSRIFELTLEKIKQNLSASGYVGYIDVNCIANGTGIYPLEFTSRFG
                     YPTLSIHMEGVTSKWGKLLYSISSGENAELKTKNGFQVGVVIAVPPFPFNDDRTFKKF
                     SEEATILFKSQNLDGIHLGEVKREGNDWHVAGRSGYSLVVTGSGTTTAEASKQAYQRV
                     ANIMIPNMFYRTDIGSGWVRDSDLLLSYGYLQ"
     gene            4595..4873
                     /locus_tag="VE308_04340"
     CDS             4595..4873
                     /locus_tag="VE308_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00235.1"
                     /translation="MWIAISIAISLALAFLLPFPFDIIAIIAVFLSINYFIRQRQMRK
                     MGLNAWSFFGSAPGSGKQSVNYYCINCGTKHNQAACPNCGSKLKKAGF"
BASE COUNT         1347 a         1061 c          982 g         1514 t
ORIGIN      
        1 tgtccggatg gtgatctctg tgacttgtga gaagttggca atgtctgtaa ggtacatgtg
       61 atagccgcaa agacatgcgg atatatacaa gtatgctgaa gcaattccca cgggatcttt
      121 accagacagc aatatattat ccccagtctg gcgggcgatt tccaacgcca gtttttgtat
      181 tcgccaatca atcctagctc tgttcgaaat cttcgcaatg tatttgtcta ggggtataac
      241 atggtcaaat gtaccgctgg ggatcttcat cttatccgct tctatagcgt tagtcatgca
      301 cattgtcatc ctcttgtaac agcgaaatgc tattctcaga gatgaccttt tgttgctgca
      361 aaaatcagtt gaatcgacga tctcttccag cccccttgca atacaagact gcttgcatgc
      421 aaggtacacc gctgcaatag cgttgccatt gactgacatg ccttttgtat tatttttaga
      481 tgaatagctt ctgtatatct tggcagcagt ctctgatagc gaccttggga ggagcaactt
      541 tgagcatatt ttgtctataa ccgagagcac cctagcgatc ctcctttcct ctgaagatgc
      601 cttcaatcta gcatggagct tacgtacaca tgcaatacgt tcggcattgg cgaaattgat
      661 cggtttgccg ttatagtctt tatctgatag gctgatttct gtctgcaacc cataattcat
      721 tagtaaagag cttgtttgcc cgctgccacg caagtttttc agcctgtcat cggaattgtt
      781 cgaatgagtt tcatgtccac agtaatccat cttctcaagc accacatagc cacaggactg
      841 gcatatgtat tcaccgtgat acacgtcaaa aataataggc gaatcgcatt ccgggcatga
      901 cggcttgatg ttctcatcat ttttcatgcc gttagcaaag gcagtgattc ctctttccat
      961 tcttatcgcc ggcaccacca tatgcaactt ttgggatcta ccaatgccga attgaattgt
     1021 gaacgctccg ctgtgctttg ctcattaagt atacttctaa ttgaatctcc tggaagtatc
     1081 atgaaaatca tttccttgta gaaatataat gaatacaact tatacttcga tgtttcataa
     1141 atttaaccta gatctaaaat atctttatac cagatcttac tatcaaatga ccgtagagga
     1201 tttttcaggg cgcctccctt ttttctcgag agtcggtcac cgcgataatc ggcgctcgat
     1261 aaaaattaac tatcaaggtt attaacacct tgcatgttgc actacaatga attgcataag
     1321 agctgcaagc tcccgagggg tagagaatgt ctagatccct agcaatcctt attttctggg
     1381 cactgggctt tacaataggt tccgcagggt atctaatggt accaaacgca gtgtcttggc
     1441 taggcaatat acttcctgaa atcgtagtga accaagccat attaggctct attttagctg
     1501 gcgtagtcgg ctcagcagta agcacattta ctgtagtgac ttgggcaaat agagctccat
     1561 aaattttttt taacttttat acaaatatac ctttattaat tgataggaca acatttttct
     1621 ggatatacta catgtcatgt atgatagaca cagttgtctt ggctatttgt cactctatct
     1681 tttcagtagt tccaaaaata agaaactgac ttggcatatg atataggttt catgctaacc
     1741 tctgtttcct cgggcggcat atcaggattc ttgatcggat atgctataaa aaagggaata
     1801 aagatcatac tcggtatagc gggccttttg ttaggaggtt tggcgtatct aaactacaag
     1861 ggattggtaa gcgttgactg ggaaagcatt gcatctatgt caaacaaggc tattacagaa
     1921 ttttctttaa gcagcgcaaa ctatgcctcc tcagtaatgg atagccaagt agtgccgacc
     1981 atgatgaact ttggagtacc attgactggc agttttgccg caggctttat cttgggctgc
     2041 ctgaagggct agcaggcaaa ctattatgct ttggggtacg attagatact aatccctcaa
     2101 gccgcttgta tagggctaga aattgcatcc cccttctgta gtattttaga cattgttact
     2161 ttcttccttt gctatcaatc ttctttccac caacatttgc agatagaact ggatctggtg
     2221 gtgagaaagg aatgctccat acataatctt gctctcagac atcctgaacg aagcaaagcg
     2281 taagatttct gcaataattg ccgtagcccg ttctatgcgt catagaaaga attctcaatt
     2341 caataagtac cacgtactaa aggtatatag tcaaaagtat taggctttat tggcaagagg
     2401 tttgtcaaga tagccaatag tatctgtaca tcttgtaaag attcctttag agtacctgaa
     2461 ttaacctatg aatgaattat ctgtgcatgt ttgtaaatgt tgtatgcgat aaatgtagtt
     2521 caatagtcta caaaatgaaa gcactgcgct ctgtaaagga aattctgagt cagtcgagca
     2581 accgctgcac aagttgtggt gctcctctga gccttggaga gtttctgatc agagccacaa
     2641 aaaattgaac caggatcata atttggtaga agtttagcct tttttcatta attgttttga
     2701 agtctatatc agtactgtcc aacttttagg cgatagacgg cttacccaat gggggcgaca
     2761 tcttctattg ctctatcgca gttcatttga agataggcac cttcactatt attcagcttc
     2821 ctgtttgttg ttcttgatca agaactttgg tttgaccttc tagccttatt tttattgtag
     2881 ataaccatat gaaagaagga ggtcgctgtc tcgaacccat ccgcttccaa tgtctgtcct
     2941 atagaacatg tttggtatca tgatattggc tactctctgg tacgcctgct tgcttgcctc
     3001 cgcagtagta gttccagaac cggtcacgac aagtgaataa cctgacctac cggcgacgtg
     3061 ccaatcgttg ccttctctct tgacctctcc aagatggatg ccgtcaaggt tctgactttt
     3121 aaaaagtata gtagcttctt ctgaaaattt tttgaacgtt ctatcatcat tgaacggaaa
     3181 tggcgggaca gctatgacaa ccccaacctg aaatccgttt ttcgtcttta actctgcatt
     3241 ttcacctgag gaaatagaat atagtagctt gccccatttg ctggtgaccc cttccatatg
     3301 aatagatagt gtaggatatc caaagcgtga cgtgaactct agcggatata tgccggttcc
     3361 gtttgctatg cagttaacat cgatatagcc gacgtaacct gaggcggaaa ggttttgttt
     3421 tattttttca agtgtcagtt caaaaatcct gcttttttgt atccacagca taagagtacc
     3481 catctcaccc gtactagggc caatgtttcc aggaaacaat cgcttgtgct caaagttaat
     3541 gttaacgggc cggatgaatt cttttccatt aaagaatccg cctacagcca cctctacgcc
     3601 agaagagtac tgttgcagtt gaaatgcctt tattttattt gaccaatttt ttttgtaatg
     3661 agcaaggaca ttgatcacgt cattgccgtc ggcctcttga ccgacaaaga gtagctcctt
     3721 ttcgctctgc gctttcccgc tgggctttat cacatatctg ccggagtttt catgtacaaa
     3781 tcgtatggcg tcgtcaaagt ttgaaaaatt ccagcttgga aggaccgaca caccagcctt
     3841 tgtaagctcc tgctggccga attctctttc attttcgagc ttgtctgtgt acagacttcc
     3901 tccaattacc ttcttgcctt cagctctcag tttctccgct atcttgccaa atccaatatc
     3961 gtcaaaaaca actacatctg cccagtctgc atgatcctcc catgaatcga ccttatcaac
     4021 aaagccgtcg ccgacgtctc tttctgtcag actctggata tataacagga catcattgcc
     4081 ctcctgtcgt atgacatagg caaggtcaac acttaaggct tcaagagata cgaagaggaa
     4141 tttcaattcc attatagaaa ggagttggaa ttttaaagac tatcggttca gcaattactt
     4201 gacgattgtc caaaactttt ttcggcagca tagagaatta gagtcgatgc ttataaagcc
     4261 ggcatcggat caaatattca tcattaccaa aaaactattc catatatagt tgttaaaaga
     4321 tgaaaggcat ttgcgcatag cacacattgg acataatcgc aaaagcagat cagtctttca
     4381 atgtgataaa agaatctaat cgccaggtct gcttgtttgc tgaacacgag cattccagcc
     4441 tagtataacg atgataattg aatacctagg ttattagctt aaggactagt aggcaacgac
     4501 ctttgatggc aaacctgcat aaaacatttt agggatgttc agctaacaat agccgttgga
     4561 tcctaatgcg aagagatttc ttaaaattca attgttgtgg atagcaatct ccattgctat
     4621 tagccttgcc cttgcttttc tccttccatt cccatttgat attatagcga taatagctgt
     4681 cttccttagc attaactact tcatccggca aagacagatg agaaaaatgg gccttaacgc
     4741 atggagcttc tttggatcgg ctcccgggtc tggaaagcaa agtgtcaact attattgcat
     4801 aaactgtgga accaagcata accaggcagc atgccccaac tgtggttcta aattaaaaaa
     4861 ggcgggattt taaattttag agaacagatg gatatacttg gacg
//
LOCUS       DASQTF010000184         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_337379,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000184 DASQTF010000000
VERSION     DASQTF010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337379"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..880)
                     /locus_tag="VE308_04345"
     CDS             complement(104..880)
                     /locus_tag="VE308_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00236.1"
                     /translation="MRWSPSRLYVVEIKMIVLAAFATVVLLFGPNAGIASAQELPSIP
                     AVIQNGTFQNTEDGFRMQVPDNWVVQDIDNLHLPNFRVANEAGFLILAIICPQQEAMP
                     GIGGLYNCEQSENSIEILHDRLGHRPEFEVIENPANITPDDFLAFMIEEMQGRNYTNI
                     RIINSTDLTINITSLEDPNTTIRTVPAKLVEMTYHPNLGLADMRSYSILATIPENPLP
                     GLRQIVSGDSITYEGPAAATPSGNLPPPVQQMFQSLEFIR"
     gene            complement(1796..>2452)
                     /locus_tag="VE308_04350"
     CDS             complement(1796..>2452)
                     /locus_tag="VE308_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZB00237.1"
                     /translation="VSAGRRNVDMVYIIFNNAVYGLTKGQAAPTLKLGMKTKSLPQPN
                     VNNSVNPIALALVAGFTFLARGYSYDVRHLKELIKKAVAHNGLTFIDVLQPCPTYNDI
                     NTKEWFQGLDNIDPKVGKAMPRMYKLEETGYDGEVHSPDEINQKMSQVIEKSNEWGSK
                     IPIGVFYQNEHVPTYQERITARIPDYMENPPALQQIADDEGKTVTSIDKLLADLQVVK
                     "
BASE COUNT          623 a          524 c          508 g          797 t
ORIGIN      
        1 attgtcctgc agaaaatcta tcttcttgct caggttgcca ggtacttcct atgctgttat
       61 tgtcatactt tactactact aaatgtagcc tcttatttga tcatcatctg ataaattcga
      121 ggctctgaaa catctgctgt actggtggtg gaaggttgcc tgagggcgtt gccgctgcag
      181 gaccttcgta ggtaatagaa tcgcctgaca cgatctgtct tagtccaggt aatgggttct
      241 ctggaatagt cgccagaatt gaataactcc tcatatctgc gagtccaaga ttaggatggt
      301 atgtcatttc tacaagtttg gctggcaccg ttcttattgt tgtgtttgga tcttcgagac
      361 ttgtgatatt gattgtgagg tctgtgctgt ttataatccg gatgttagta taattacgtc
      421 cttgcatttc ctctatcata aatgccaaga aatcatctgg tgtgatgttg gctgggttct
      481 cgatgacctc aaattcaggt ctgtggccta agcgatcgtg tagaatttct atggaatttt
      541 cagattgttc gcagttatac agtcctccaa taccaggcat tgcctcttgc tgcgggcaaa
      601 ttattgccaa tattaaaaaa cctgcctcgt tcgcaacacg aaagttggga agatgtaaat
      661 tgtcaatgtc ctgtactacc cagttatctg gcacttgcat tctaaagccg tcctctgtgt
      721 tttgaaatgt cccgttctga atcactgctg gtatactggg cagttcttgc gcgcttgcta
      781 ttcctgcatt gggaccaaaa agaaggacaa cagtagcaaa tgctgctaga actatcatct
      841 ttatttccac tacgtataag cgcgatggtg accacctcat tgccttcaaa taatatacac
      901 atcccagagc cacataaggt tatggattaa tattctctac tgattagaac taatcctgcc
      961 tatttgggtt tagcaggttc aaagaatcaa ctactacatc ccagtgcttc tctatcattc
     1021 ccttctcgat tctaaatagg tcagcacgag ttcctgatac aattgttttg ttggtaggca
     1081 gcgccctcaa gtattcccac gtatgtgtaa ctgttgtgtt ttagaaatgt cataactaga
     1141 tcatcttcag taatcatatg ctctacggtt gcatgagggt ctgggaagcc ctcgaagaag
     1201 ggaccagaga attgcttaaa gcctttgatc ccgtttccta tctgtggatt gtattgtata
     1261 agttcatgtg cgtagtactt atataatgca ctcatattat gttcgttgaa gatttcttct
     1321 ataaatgatc tgacaagtac cttattgcac tctcatccat catcgagtga gatggtgacg
     1381 gcgactgctg ctgtattttt attgctgtcg tcatattcgg aggttgcaac tgcgctagtg
     1441 caaaaagtgg gacgacaatt agaattcctg aggagagcgt tgctagtaac gctgatggtt
     1501 atacataatc ataataacat aaggatctgt tgtcatttac ttctacagaa taggtagtgt
     1561 cggtctcaaa tatggtgctt atatgaaaga tatttctaga tgaatctgaa attaataatt
     1621 ttaataggac agcctttcaa ctatttgaaa aattcaaacc tagatcgagg gataagattg
     1681 ctatttcctt ttcacatagt actccgacag caaaagtatg atatcatctt aagcccacct
     1741 ttttaaggct cttgtaacat tactgattta cttaagattc tctttatgat gcgtgtcact
     1801 taaccacctg aagatccgca agcagcttat ctatgcttgt gaccgttttg ccctcatcgt
     1861 cggcaatttg ctgcagggca ggtggattct ccatgtaatc aggaatgcgt gctgttatcc
     1921 tctcttggta ggtaggaaca tgttcgtttt gataaaaaac accaatcggt atcttactgc
     1981 cccactcatt tgacttttca atcacttgag acattttctg atttatctca tcagggctat
     2041 gcacctcgcc atcgtaccct gtttcttcca gcttatacat cctcggcata gctttgccca
     2101 ctttcggatc aatgttgtca agcccttgga accattcctt ggtattgata tcattatacg
     2161 tagggcatgg ttgtaatacg tctatgaacg tgagtccatt atgggcaact gccttcttga
     2221 tcaattcctt caggtgacgg acgtcgtagg aatagcctcg tgcaagaaaa gtaaagcctg
     2281 caacaagcgc tagagctatc ggattgacag agttgttaac atttggctgc ggcagcgatt
     2341 ttgtcttcat gcccagcttg agcgtcggcg cagcctgtcc ctttgtcaac ccatatacag
     2401 cgttattgaa tatgatgtag accatatcga catttcttcg ccctgcgctc ac
//
LOCUS       DASQTF010000185         7122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_139581,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000185 DASQTF010000000
VERSION     DASQTF010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139581"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..706
                     /gene="pdxT"
                     /locus_tag="VE308_04355"
     CDS             50..706
                     /gene="pdxT"
                     /locus_tag="VE308_04355"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZB00238.1"
                     /translation="MNNSSSNKKKSVTIGVLGFQGDIEENVAATTQALQELQMDGNVE
                     MVRYPYEIENIDGLILPGGESTVQSTLIAIQQSQPVLKKRISEGLPVLGTCAGMIMLS
                     KRAYDRVIGDTKPKLIGNLDIVIERNAFGRQNDSFEADLSMTMLGKEAFKGVFIRAPA
                     VSEVGKDVGVIGKLNNRIVAVKQKNIIGTAFHPELSGDSRIHKHLIEMALEFKNKNGS
                     "
     gene            complement(757..933)
                     /locus_tag="VE308_04360"
     CDS             complement(757..933)
                     /locus_tag="VE308_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZB00239.1"
                     /translation="MSAIEWHQVDTSESAIDAIDYIRQNYDLIVSDIRMQQMTGFEFV
                     RQIRKSCSIQRYYS"
     gene            complement(1390..2484)
                     /gene="cax"
                     /locus_tag="VE308_04365"
     CDS             complement(1390..2484)
                     /gene="cax"
                     /locus_tag="VE308_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HZB00240.1"
                     /translation="MLKSVKLTKSSAIYILLIFAPIAAALEYVHADDLVLFVISAIAL
                     IPLAKLIGDSTEHLATHYGPTAGSLLNVTFGNAAEIIIAVIAISAGLLDLVKASITGS
                     IIGNILLILGLSVIAGGFRFKEQTFSRENVGIQASMLFLAIIGLAVPTILASTMSSSS
                     ENQGQVQLLSDALAFILLIVYILGIVFTFITHKHLFTPPETESMHESHGTHWSKKKSF
                     LFLAASMAGVIVVSEMLVASVETVGEELGFSELFVGAIIVGIVGNAAEHSSAILLARK
                     GKIELSIGIAAGSGTQIALFVVPILVFAGIAMGQPFTLVFTLLELAVIFLGAIILNLI
                     VHDGRSNWFEGIMLTAVYIIIAIAFFFVAD"
     gene            complement(2735..2878)
                     /locus_tag="VE308_04370"
     tRNA            complement(join(2735..2778,2800..2807,2846..2878))
                     /locus_tag="VE308_04370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2802..2804),aa:Leu,seq:tag)
     gene            2926..4326
                     /gene="glmM"
                     /locus_tag="VE308_04375"
     CDS             2926..4326
                     /gene="glmM"
                     /locus_tag="VE308_04375"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZB00241.1"
                     /translation="MATVKNNNIDMQASNVKPRLFGTNGVRGVYGQELTYDLVIDLCY
                     ALGTYFGKGPIVVGYDGRKSHPVLSRLVRSTLNSAGLDTADTGLVPTPCLQYAAKRLG
                     YNGGVMITASHNPPEYNGIKPTAADGVEIPREDELKVEDIYYSKRFVRIDGTGHDSID
                     YNIISRYIEAVLALIDVNRIRQQKFTVVMDTGNGAQAVVAPLIAKKLGCKVIIINGHI
                     DGDFPSRGSEPTPDNLEVLSETVKSARADFGVAYDGDGDRSIFCDNTGTIYMGDKTGA
                     LLVKHLLSQKHKGSEVVCPINTSMILSIVAEQAGSKTIYTKVGSVEVSREMVKRSCAL
                     GLEENGGFMYGPLNEVRDGAMATALVLDMMAASDKSFSQHIAQLPQTFQYKTKFTCSS
                     REVVDRVIKICREHGSPKKVETLDGAKIWIDDETWLMLRPSGTEPLIRMYAESTDKSL
                     LDSKVDEYRRYVQSLI"
     gene            4402..4791
                     /gene="rpl7ae"
                     /locus_tag="VE308_04380"
     CDS             4402..4791
                     /gene="rpl7ae"
                     /locus_tag="VE308_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HZB00242.1"
                     /translation="MSKPYYVKFETSKDLVNAVYEAVRVSKQSGKVRKGTNETTKAIE
                     RGVSKLVVIAEDVEPPEVVAHLPILCEERKAPFIFVPSKQQLGASLGIDVGSAAASIV
                     DAGEAQHIVEQVVSSISTMKGVAAAPK"
     gene            4809..5027
                     /locus_tag="VE308_04385"
     CDS             4809..5027
                     /locus_tag="VE308_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HZB00243.1"
                     /translation="MSSKTAEEKVVPAEVIQIVGRTGIAGEVIQVRVKVLEGKDRGRI
                     LTRNVKGSIRMNDVLMLRETEREARKIR"
     gene            5037..5252
                     /locus_tag="VE308_04390"
     CDS             5037..5252
                     /locus_tag="VE308_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HZB00244.1"
                     /translation="MSKTATSLRNCSFCGRHITSGHGIMLVKNDGHVQWTCSSKCKKN
                     MRVLKRDPRKLKWTVRYVKGGLHTKKQ"
     gene            5266..5670
                     /gene="ndk"
                     /locus_tag="VE308_04395"
     CDS             5266..5670
                     /gene="ndk"
                     /locus_tag="VE308_04395"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZB00245.1"
                     /translation="MAPEQTLIVVKPDGFRRHLTGKILARFEEKGFQLRNLKSYNFTK
                     EKAQEFYSAHKNKPFFDELLSFIISGTVVACILEGSNAVNTVRLMVGSTKSFEAAPGT
                     IRGDFGLGFTDNIIHASDSPESFIKESRVIFG"
     gene            5680..>7122
                     /gene="infB"
                     /locus_tag="VE308_04400"
     CDS             5680..>7122
                     /gene="infB"
                     /locus_tag="VE308_04400"
                     /inference="COORDINATES: protein motif:HMM:NF003078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZB00246.1"
                     /translation="MELRQPIVVVLGHVDSGKTSLLDNIRGTAVQAREVGGITQHIGA
                     SFLPIETIKEITGPLYAKLAKAEAAIPGLLVIDTPGHEIFANLRTRGGSAADIAIVVV
                     DVNKGFEAQTIESMDILKKRRVPFVVALNKVDRVGGWRTSTRFISEDVKRQDTAVQTL
                     LDEKIYSVVGMLSRLGYPSEAFWRVKDYTKEVAVVPVSARTGVGIAELLAVMVGLTQQ
                     YMGKKLERHTKDAARGIVLESSEETGLGPSANIILLDGVLHQGDSIVVGKRDGAISTR
                     IKALLLPKPLDEMRDPRDKFKQVNEVIAAAGLKITSPDLEGVLAGSPIYVYNNGQRKS
                     KDELDRLKSVVESEVKNAIVSNTQTSGVVLKCDTIGSLEAIVDLLKKANVDIRMADIG
                     NITRRDVVEAAAVKENDRYLGVMLGFNVKVLEDAQKEAQDRGVKIFNEQIIYNLVRSY
                     TDWVAYQREHEDLIIFNELPPVCKFQFLKGF"
BASE COUNT         2099 a         1491 c         1724 g         1808 t
ORIGIN      
        1 tggacacaaa taatcttgag cttcgtatgc aggaaagagg cgagcactcg tgaacaatag
       61 tagtagtaat aaaaagaaat cagtcacaat tggagttctt ggctttcagg gagacataga
      121 ggaaaatgta gctgccacaa ctcaggcgct gcaagagctg cagatggacg ggaatgtcga
      181 gatggtacgt tatccatacg agatcgaaaa tatcgacggc ctgattttgc caggtggcga
      241 aagcactgta cagagcacgc ttatcgccat tcagcagtcg cagcccgttt tgaaaaagcg
      301 tatctcagaa ggtctacccg tgcttggcac atgcgctggc atgataatgc tgtccaagag
      361 agcttatgat cgagtcatag gagacacaaa gcctaagttg ataggcaatc ttgacatcgt
      421 gattgagcgt aacgcctttg gaaggcaaaa cgactcattt gaagcagatc tttcaatgac
      481 catgctgggc aaagaggcat ttaaaggagt ctttattagg gctccagcgg taagtgaggt
      541 aggcaaggat gtcggggtaa tcggcaagct taacaataga attgtggcag ttaaacaaaa
      601 aaacatcata gggaccgcat ttcatcctga gctctcagga gactcacgga tacacaagca
      661 tctaatcgaa atggcccttg aattcaagaa caagaacggt tcctgagaat atctccctta
      721 gtaattctca ctcagacttg tttatttcaa atgctgtcat gagtaatacc tttgtattga
      781 gcatgatttt ctgatctgtc gaacaaactc aaatcccgtc atctgctgca ttctgatatc
      841 tgacacgatc aagtcatagt tttgcctaat gtaatcaatt gcatcaattg cactttcaga
      901 tgtgtcgact tggtgccatt ctatagccga catttcatag ttatgagtat gtcttagtcc
      961 tcgttaacga tcagaattct cttgctcttt gagtgttgag atggtgatat gctcaatcca
     1021 caaatgccat ttaattacat aaataattga gcaaaagcgc gcctttaggc tgtgtcatat
     1081 tgaaagcaaa tgcaattcaa ctaattcttt tatagctatt tttgtccaaa agagccttta
     1141 ttgctgccag catgactagc ctctagatgg gcactgcatc aaagtgcttg aatcctgata
     1201 aatggatagc ctcgatgaca aagaacagca acgacttttt tgaagttggc tacccaatat
     1261 tttttactag agacgtcttg atatattcct gttccttagg atagaacgca aagcatcaca
     1321 tctgtcgtgg tcaggtctct gccctacaca cataaaaaat ttcatcgacc acctgagcag
     1381 attcaaggac tagtctgcga caaaaaagaa tgctatcgca attatgatat agactgctgt
     1441 cagcattatg ccttcaaacc aattgcttct accatcatgg actatcaagt tcagaattat
     1501 agctcctaag aaaattactg ctagttccag cagtgtaaac acaagtgtaa atggctggcc
     1561 catcgcaatg cctgcaaata caagaattgg gactacaaaa agtgcaatct gggtacctga
     1621 gcctgctgct atgccaattg aaagctctat tttgcctttc cttgcaagga gaattgcact
     1681 actgtgctca gctgcattac caacaatacc tacaattatt gcaccaacaa acaattcgct
     1741 aaagccaagt tcttcaccta ctgtctcgac tgaagcaact aacatttcgc ttactactat
     1801 gacccctgcc atacttgctg caaggaatag aaacgacttt ttcttgctcc agtgtgtgcc
     1861 gtgactttca tgcatgctct cagtttcagg cggtgtgaac agatgtttgt gggtaatgaa
     1921 cgtaaatact atcccgagga tgtagacgat cagcagaatg aatgccaatg catcgctcag
     1981 caactgtacc tgcccttggt tttctgaaga cgaagacatg gtgcttgcca aaatggtcgg
     2041 tacggcaaga ccaattattg ccaagaaaag cattgatgct tggatgccaa cattttccct
     2101 gctaaaggtt tgctctttga atctaaagcc acctgcaatg acactcaacc ccaagatgag
     2161 aagtatatta ccaatgattg aacctgtaat ggatgccttg accaagtcta ggagtcctgc
     2221 gcttatggca atcaccgcaa taatgatctc cgcagcatta ccaaaggtca cattcagaag
     2281 tgaccctgct gtggggccat agtgggttgc aagatgttct gtggaatcgc ctatcagttt
     2341 tgccagcgga ataagcgcta ttgctgatat tacaaatagg actagatcat cagcatgaac
     2401 atactctaat gcagcagcta ttggtgcaaa tattagaaga atgtagatag ccgaagattt
     2461 tgttagcttg acagatttga gcaactaaga gacttttaat attatttctg cggtttgctt
     2521 taattgtttt tatgacaatt gctatgttgt cccccttcga tactatcatg ccattaactg
     2581 tgcttttgag tggcacaaac ttaatacata aatgaagtaa acaggtaata tcccacctct
     2641 gaagtttttg tagttcaatt cttgtcagat caacgatcca atttgcaaat agcttgacat
     2701 acctgatgtt catgttgttg ttatgaaatt aaagtgcggg gggtgggatt tgaacccacg
     2761 aatccctaag gaacagggtt gcaggaacca gccggctcat tctaagcttc acaccctttt
     2821 ccttttttcc caaaagaaaa agagacctgc gccgttgacc gggcttggcg acccccgccc
     2881 gcctaagaat gcaagaaccg gcagccaaat ttatctattt atatcttggc aactgtaaaa
     2941 aataacaata tcgatatgca agcaagcaat gtcaagccgc ggctctttgg tacaaatgga
     3001 gtacgcggag tttatggtca agagcttaca tatgatcttg tgattgatct ctgctatgca
     3061 cttggcacat actttggcaa agggcccata gtggtaggat atgatggacg caaatcgcat
     3121 cctgtcctat caagacttgt caggtcaacc ctcaactctg cggggcttga cactgcagac
     3181 acagggcttg taccgactcc gtgcttgcag tacgccgcaa agcgcctagg atataacgga
     3241 ggggtcatga taactgcatc gcataacccg cccgagtaca acgggatcaa acctactgcg
     3301 gcagatggcg tagaaattcc aagagaagat gagctgaagg tcgaggatat ttattattca
     3361 aagagatttg tgaggataga tggaactggt cacgattcta ttgattacaa tattatcagt
     3421 aggtacatcg aggctgtgct tgccttaata gatgtcaaca ggataaggca acaaaaattc
     3481 actgtggtga tggatactgg aaatggagcg caagcagttg ttgctccgct tatagcaaaa
     3541 aagcttggtt gcaaagtaat catcatcaat ggtcatatag atggcgactt tcctagccgt
     3601 ggatcagagc cgacaccgga caatcttgaa gtgctctcag aaactgtgaa atctgcaagg
     3661 gcagattttg gtgttgcata cgacggcgac ggcgatcgta gtatcttttg cgataacacc
     3721 ggcacaatct acatgggcga caagactggc gcgctcttgg tcaagcactt gctgtcacaa
     3781 aagcataagg gctccgaagt tgtttgtcct attaacactt caatgatact atctattgtg
     3841 gcagaacagg ccggctctaa aacaatttac accaaggttg gaagcgtcga agtatctcga
     3901 gaaatggtca agcgcagctg tgcacttggg ctggaggaga atggcggctt tatgtacggg
     3961 ccgctaaatg aagtaaggga tggagcaatg gctactgcac ttgtacttga tatgatggct
     4021 gcatctgaca agtcattttc acagcacatt gcgcagctgc cacagacctt ccagtataag
     4081 accaagttta cgtgttcttc acgagaagta gtcgacaggg taattaagat atgccgcgag
     4141 catggaagtc ccaaaaaggt cgagacgctg gacggagcca agatttggat agatgatgag
     4201 acttggctga tgttaaggcc tagtggaact gaacctctta tcagaatgta tgcagaatca
     4261 actgataaat cgctactgga ctcaaaggtt gatgaatacc gcaggtatgt gcagtccctg
     4321 atataggaat ggacaacgtt tttaatacgc ttattcttct gtgtaagaag aataagatga
     4381 tcccaatggg tgatagaaac aatgagtaag ccatattatg tcaagtttga aacttctaag
     4441 gatctggtaa atgcagttta tgaggctgtt cgggtatcaa agcagagcgg taaagtccgc
     4501 aagggcacca atgagaccac caaagctata gaacgcggtg tatcaaagct agttgtaatt
     4561 gccgaggatg tcgagccgcc agaagttgtg gctcatctgc ctatcctctg cgaagagaga
     4621 aaggcaccat ttatctttgt tcctagcaag cagcagctgg gtgcatcact tggaatcgat
     4681 gtgggctctg cagctgcctc cattgtcgat gctggcgagg ctcaacacat tgttgagcag
     4741 gtcgtcagct ctataagcac tatgaaggga gtggcggcgg ccccgaaatg ataggtggaa
     4801 actgatagat gagcagcaag acagctgaag aaaaggtcgt ccctgcagaa gtaatccaga
     4861 tagtgggcag gacagggatt gccggcgaag tcatacaggt acgtgtcaag gttctcgaag
     4921 gcaaggatag aggccggatt cttacaagaa acgtcaaggg ttctattagg atgaacgatg
     4981 tcttgatgct tagagaaacc gagcgcgagg cccgcaagat aaggtgataa tggttaatga
     5041 gcaagacagc aacgtctcta cgcaactgct cgttctgtgg tagacatatc accagtggac
     5101 atggaatcat gcttgtaaaa aatgacggac atgtgcagtg gacttgctcg agcaaatgca
     5161 agaagaacat gcgggtgtta aaacgtgacc caagaaaact caaatggaca gttcgctatg
     5221 taaagggcgg actgcacacg aaaaagcagt agtggtagat aggctatggc acctgagcaa
     5281 acgctaattg ttgtcaaacc tgatggtttt aggcgccatc tgacgggcaa gatattggcg
     5341 cgctttgaag aaaaaggctt tcagctaagg aacttaaagt cttataattt caccaaggaa
     5401 aaggcacagg aattttactc tgcccacaaa aacaagccat tttttgacga attgctatcc
     5461 tttattattt ccggcacggt cgtggcatgc atcctagagg gcagcaatgc agtgaacaca
     5521 gtaaggctca tggtcgggtc gaccaaatca tttgaagcgg ctccaggaac catcaggggt
     5581 gactttggcc tagggtttac cgacaacatt attcacgctt cggattctcc tgaaagcttc
     5641 ataaaagagt cgcgtgtcat ttttggctga tctatatacg tggaacttag gcagccaatc
     5701 gtcgtagttt taggtcatgt agactcgggc aagacttcat tattggacaa cattcgtggc
     5761 actgcagttc aggcacgaga agtaggcggt ataacacagc acataggtgc aagttttttg
     5821 ccaattgaaa ccattaaaga gataactggt ccgctatacg ctaaacttgc caaggcagaa
     5881 gcggcaatac ctggactgct tgtcatcgat acacctggcc acgagatttt tgccaacctg
     5941 cgcacacggg gcggctctgc tgccgacatt gctatagtcg ttgtcgatgt gaacaagggt
     6001 tttgaagctc agactattga aagcatggac attctgaaaa agcgcagggt accatttgtc
     6061 gttgcgctca acaaagttga cagggttggt ggctggcgca cgtctacacg attcatttct
     6121 gaagatgtaa aaaggcagga taccgctgtg cagactttgc ttgacgaaaa gatatacagc
     6181 gttgttggaa tgctctcgag actgggctat ccatcagagg cattctggcg agtcaaggat
     6241 tacacaaagg aagttgcggt agtccctgtc agtgcaagaa ccggcgtagg aatagcggaa
     6301 ctcttggccg taatggtggg tctaacacag cagtacatgg gaaagaaact ggagcgccat
     6361 accaaggatg ctgcaagggg tatagtgctt gaatctagtg aagaaacggg acttggaccg
     6421 tctgcaaaca tcatacttct tgatggagta ctgcatcagg gcgacagcat cgttgtaggc
     6481 aagcgcgatg gtgcaatttc taccaggata aaagctttgt tgctgccaaa gccccttgac
     6541 gaaatgcgag accctcgaga caaattcaag caggtcaacg aggtaatagc cgctgcaggc
     6601 ttgaagataa cctcgccgga ccttgagggg gtgctggcag gaagtcctat atatgtttac
     6661 aacaacggtc agaggaagag caaagatgag cttgatcgcc taaagtcagt tgtagagtca
     6721 gaagtcaaga acgccatcgt aagcaataca caaactagcg gagtggtatt gaaatgcgat
     6781 actatcggct cgcttgaggc catagtagac ttgctcaaga aagcaaatgt tgacatcagg
     6841 atggcagaca ttggcaacat tacaagaaga gatgttgtag aagccgctgc agtgaaggag
     6901 aatgaccgct atcttggagt tatgttaggc tttaacgtca aagtgcttga agatgcacaa
     6961 aaggaagcgc aggatcgtgg agtgaagatc tttaacgagc agataatcta caatcttgta
     7021 cgaagctata cagactgggt ggcataccaa cgtgaacatg aagacttgat catattcaat
     7081 gagctgccgc ctgtatgcaa attccagttc ctcaaaggat tc
//
LOCUS       DASQTF010000186         4828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1173090,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000186 DASQTF010000000
VERSION     DASQTF010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173090"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..610)
                     /locus_tag="VE308_04405"
     CDS             complement(308..610)
                     /locus_tag="VE308_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00247.1"
                     /translation="MVIITLQQIIDEKISFLREQINPNNKPELNSTFQIQIDTIRSAA
                     QDLENVESIIMQKKEVLKNSKDVKETDRLLAELEALEWPQAQIVVFTLDNREAVGY"
     gene            760..1143
                     /locus_tag="VE308_04410"
     CDS             760..1143
                     /locus_tag="VE308_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00248.1"
                     /translation="MADTPTLPNEPTEQEEEQQVLQFIQDFHDNLTCECMGDSKTCYY
                     EYAKIDGKYYKRDTTYYDVNELCHDCGVENGDGNIHHGTCDIERCPACGGQFLLDDNH
                     EEIELLLRLPKGAKLTPSVKIDLDF"
     gene            complement(1334..1558)
                     /locus_tag="VE308_04415"
     CDS             complement(1334..1558)
                     /locus_tag="VE308_04415"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZB00249.1"
                     /translation="MLTQSKKYRSRTEIICDILQAARSDGNGIVKTKIMYSAFLSYHQ
                     ITEYLTLLIDNGLLQYDIGNQKFRITEKGL"
     gene            complement(2227..2379)
                     /locus_tag="VE308_04420"
     tRNA            complement(join(2227..2240,2272..2293,2341..2379))
                     /locus_tag="VE308_04420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2342..2344),aa:Arg,seq:tcg)
     gene            2620..2814
                     /locus_tag="VE308_04425"
     CDS             2620..2814
                     /locus_tag="VE308_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00250.1"
                     /translation="MQIYILVTIWFAGIVTTGIALLFEDYSNYAVIGTAGWTIVAASS
                     GMIFYEIKRIRSEDRKKELV"
     gene            complement(2816..3064)
                     /locus_tag="VE308_04430"
     CDS             complement(2816..3064)
                     /locus_tag="VE308_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZB00251.1"
                     /translation="MPTGYILVNCTLGSEEKIINEIAKLPDAKEVRGTYGVHDIFVKV
                     KSENTETMNHTITNKIRKIPGITSTVTLVVIEEQGGKG"
     gene            3572..4546
                     /locus_tag="VE308_04435"
     CDS             3572..4546
                     /locus_tag="VE308_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZB00252.1"
                     /translation="MGQPGKIAFPVILVLGAITGYITYTLFTAAIPDESRIDSPYYQP
                     LVPGSSGNVPGDTGGAAQQQEGGGAASTGAGTTTITILQGSAVQGSPDYDPDTAQVPV
                     GNRVVWHNEDTVPHTATSGNDPQDPQSGQLFDTSLINGGEESTPVELQGVSEGQKIPY
                     HCMVHPYMAGEITITAAAGGGGQGDVGGGSAQTQGGGATNQTGANATTTTGANATTGA
                     STTSAGGSNSVSIVPGSSSLTDTAYQPNPIQVSVGATVTWTNDDPQPHTVTSGSNGQP
                     DNKFNSSPNFTPLLNAGQTFSFTFAQAGDYPYFCMLHPNMVGTVSVSG"
BASE COUNT         1455 a          994 c         1028 g         1351 t
ORIGIN      
        1 acaatcaaat gtagcccaac cgtatcaagt tgcaatgctt tcaactcttc tggcaggggg
       61 attctctacc gttacagcaa acttgatgat aaaggcacaa aagctattct agcgaatgga
      121 caggtatacg tacgtgcgtg cactctcatt ctcttcttcc gtcaagagat atatctacaa
      181 caaccaacct ctttactaat ctgcacttac tatcatacag ttccagcaag ttaaagggtt
      241 gtcacttatc ctatcaaact tctttagcgt cttatcgcat acgctacagc gaaagtagta
      301 gtatccacta atatccaacc gcttccctat tgtcaagagt gaaaaccact atttgggcct
      361 gcggccattc taaagcttca agctctgcaa gcaacctatc cgtctctttt acgtctttgc
      421 tattcttcaa tacttctttc ttttgcataa ttatggattc aacattttcc aagtcctgag
      481 cagcagacct tatagtgtct atttgtattt ggaatgtact atttagttct ggcttgttat
      541 tcggattaat ctgttccctt aagaatgaaa ttttctcgtc aattatttgc tgtagggtta
      601 tgataaccat atatggagaa caacctaaga aacagatggc ggattattaa acaaactagc
      661 accttagggg gagggagtcg cgataattga ccgagtacgt accctccccc tcaagtgcac
      721 cattcttaat ataaatatgc tccctgcttg tatactggga tggcagatac gcctacttta
      781 ccaaatgaac cgacagaaca agaagaagaa caacaagtcc tacaattcat acaagatttt
      841 catgataacc tgacctgtga gtgtatgggc gacagcaaga cgtgttatta cgaatacgca
      901 aagatagacg gtaaatatta caaacgagat actacgtatt atgacgtaaa cgaactttgc
      961 catgattgtg gtgttgaaaa cggtgatgga aatatacatc atgggacttg cgatattgag
     1021 agatgtccag cctgcggcgg ccaattcttg ttagacgata atcatgaaga gattgagtta
     1081 ctactaagat tgccaaaagg tgcaaagcta acaccaagcg ttaagataga tttggacttt
     1141 tagattagat tgaattgatt tgactggatt agatttagca gtagctcaca gatggaaagt
     1201 tactttcgtt attacttgac cttaaagtaa gcaaattatg acctgactat ccaatagctg
     1261 gccttttctc atcttcaaag tcacttaggg gccttctatt aaatcgccta tctggtcgca
     1321 tagttgaaga aacctaaaga cctttttcgg taatcctgaa tttctgatta cctatgtcat
     1381 attgaagcaa gccattatct atcaaaaggg tcagatattc cgtaatttga tgatatgaga
     1441 ggaatgcgct gtacattatc ttagtcttta caataccgtt gccatcactt ctagcagcct
     1501 gtaaaatatc acagattatc tctgttctgc ttctgtattt cttgctctgt gtcagaattt
     1561 tcctcccctt ctgctacatc cctcagtatc cacagtcgtt gtcttaattc gtttgccatc
     1621 tatcgccgat tatcttttga gaggtcaaaa tgctaaatag attattagac atcattatag
     1681 ctaaagtagt caaatgtttg atatatttgc gtaaaccagc agcgaaagga gagagggttt
     1741 acgtaggtag ttacttgcat acgaagtact aagaacatac gcataaaagg aatttgtagt
     1801 attacagaca aaacaatagt gatagcgata tggctttgga gtttgagcag gcgataaaag
     1861 tgttagaggg gcatataaaa attctaaagc agaatctccg tttctttatg gtaaactata
     1921 aagctgaata tacgacccaa atcataacaa ttaagagaga actggataaa gccgtgcagg
     1981 taagaatgaa gttaagtcag atttattatc atccaacaac aatagggtgg ccgtttctgc
     2041 agctgcttag tgcttaatac aacgtaaaag ttgttagcat ttgcgatgca aatgatattt
     2101 agatagcagt cagccgaagc agaggctatc tcggggttgg gggctatgca tgcacggggc
     2161 tccgacagtt taggccctca taaggggctg gcctttgagt aggcaaatat gacaagaata
     2221 aaatagcggg cccggtgggc ataccggatg atgataacgg aatcagcatc tttcggtccc
     2281 acgacctgcg gttatcttca ccagcagctt catcccttta taaaggggtt cacgccagtt
     2341 ccgaagaccg ccgcgatatc ctgactacgc tacgggccca acaagcatgt tgaataatga
     2401 agtcccatat atcaacgaaa tagtaataat gaacttagct tgtctatcaa tagcatatat
     2461 gctccaagtc ctgcgacaaa tgttctgcga tcataaagaa tcatttgtct atcgtaagta
     2521 tgaggaattc caatttatcg aattctgtaa ctgcagtagg tgtggcaaga cgctcagaca
     2581 ctttttcggc tttgatgagg atataagggg ctaaaggtaa tgcagatata tattctggtc
     2641 acaatctggt ttgcaggaat tgtaactaca ggcatcgcac tcttgtttga agattattct
     2701 aattatgctg taattggaac ggcgggctgg acaatagttg cggcaagctc gggaatgatc
     2761 ttttatgaaa taaagagaat aaggtcagaa gacaggaaaa aagagctggt ctaatctagc
     2821 ccttgccgcc ctgctcttca atgacaacaa gcgttacagt tgacgtaatg ccaggaatct
     2881 tgcgtatctt gtttgtaatt gtgtggttca ttgtctcagt gttctctgac ttgaccttga
     2941 caaatatgtc atgaacgcca tatgtacccc gcacttcttt ggcgtcaggc aacttagcaa
     3001 tttcattaat tatcttttcc tctgagccga gcgtgcagtt cactagaata tatccagttg
     3061 gcatatgtct gatggaagca aaagttagta tttagtcttt gctacccttt tgcgatgttt
     3121 tcttgtaaga tgcaatgtag tgctcagaca acttcctgtt ataacactga ccacatatta
     3181 ggccagagat attccattca ggcatcggcc tataacgaaa agtaaggttg ctgtcgcaca
     3241 gtgtgcaatg catagaaggg ttattgttgt taccgcccat gcgcacagac agtgtgtctc
     3301 ttatgcctag aataaaaggt tattccaagt catatgcagt atagatggtg tgtgtcatgc
     3361 aaaacggaca gatgagggca aaagttgctc caaccatgtc atgcgatatc tcctgcctgc
     3421 tggtgtcaac tgcaggtatt agaaattttc taaaacagct cttgcaggtg accatttttt
     3481 gttgtgtcat ctctaggagt gcacaacatc ttggaataaa gtcttgatcc agaaggaatt
     3541 attaaggggt attggctagc gagtagcggt tatgggtcaa cccggaaaaa tcgcttttcc
     3601 tgtaattctt gtactgggtg cgattaccgg ctatatcacc tacaccctct ttacagctgc
     3661 aatccctgac gaaagtagaa tcgattcgcc gtactatcag ccccttgtgc ctggctcgag
     3721 tggaaatgtg cctggcgata ccggaggagc tgcacaacaa caagaaggag gaggagctgc
     3781 ttcaaccgga gcaggtacca ctaccatcac aatacttcaa ggctcagcag tacaagggag
     3841 ccccgattat gatccagaca cggcacaggt gcctgttggc aatagggtag tttggcacaa
     3901 cgaagataca gttcctcata ctgccacttc tggtaatgat ccccaagatc cacagtcagg
     3961 acagttgttt gatacaagtc ttatcaatgg aggcgaagaa tcaactccag ttgaacttca
     4021 gggtgtaagt gagggccaga aaatccccta tcattgcatg gtccacccat atatggcagg
     4081 tgagattaca attaccgcag cagctggagg aggaggacaa ggtgatgtgg gtggaggctc
     4141 tgcacaaaca caaggaggag gagcaactaa ccaaacaggt gctaatgcaa caacaacaac
     4201 aggtgctaat gcaacaacag gggcaagcac aactagtgca ggtggtagta atagcgtttc
     4261 catagtacct ggctcttcaa gccttactga tactgcatat caaccaaatc caattcaagt
     4321 cagcgttggt gcaacagtaa catggacaaa cgatgaccca caaccccata cagtcacatc
     4381 aggcagcaat ggacagccag ataacaagtt caattcctca ccaaacttta ctccattgct
     4441 gaatgcagga cagacgttta gctttacatt tgcacaagcg ggcgactatc catatttctg
     4501 tatgctgcat ccaaacatgg ttggaacagt cagcgtaagc ggctgacttt ttctctttaa
     4561 ctaaaaatct ttcttttaaa aatagcgata tcacttgcac aagtcaaccc agggcagtat
     4621 ggctattatt acatggtgca tccatacatg acagggaaga tagtggtgca ataagaacca
     4681 gccatacacc tgtaaattta gctataggag gatgagaata tcttgatcaa aagaataaaa
     4741 gttgacagat agcagcttaa ggaataactg taactttagt accatagaat ttgagggcta
     4801 tcaaagaata tggacaattt aaatatta
//
LOCUS       DASQTF010000187         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1083241,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000187 DASQTF010000000
VERSION     DASQTF010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083241"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VE308_04440"
     CDS             complement(<1..1064)
                     /locus_tag="VE308_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZB00253.1"
                     /translation="MKVIQTFRISFKALGDRKVRTSLTILMVIVGSSLMVTLNGIVAG
                     LGQFATDVFSRLAPNILFVTSIPIDGPGQRDDADNGDGPPTFLGQGLIPVPAITLDQT
                     VVDKLESLAGVSAVIPSYQARITIAVENRSQSASVLSVQPENLKIIAPTLEFTEGSTI
                     DPKDESAIYLPSLIVNNLWVSELSEEAAANNDDGSTAAITIGQRVNVTYTYIDLATAF
                     RQSQSEVFTVAGIMKPVGNPTIDRAVVFNLDAGNELLQKSGRYDSLFVAAESTDHVTQ
                     VEKEIRDLYGSDIGITTSKAIIQSIGEFTAGFGSVISSIALVALLVGSVGIVTTMYTS
                     VTERTREIGVMKAIGARDRNV"
     gene            complement(1070..1987)
                     /locus_tag="VE308_04445"
     CDS             complement(1070..1987)
                     /locus_tag="VE308_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZB00254.1"
                     /translation="MFKDPVCNMMVDEENTKFVSESGGRREEEAAGKQSHCYLPSHLQ
                     STVAVALKLENVKKVFDSPSASAKMVVALNGVSFEVKGGEFVSIVGPSGSGKSTLLNV
                     IGALDRPTSGKVFINGQDIFLLDDTRLSDVRNHLIGFIFQSYNLVNRMSVQENVEFPA
                     VFSERLSSARSHPRALELLEILGIKDKAKQKPVDLSGGEQQRVAIARALINDPALVLA
                     DEPTGNLDTNTGREVFDLLKMLSDRFGTTVVMVTHNLELAGMTTRSIYIRDGRIEKEI
                     MHKTTSKTEARPTDDDDANKDIISEEKAR"
     gene            complement(2198..>2537)
                     /locus_tag="VE308_04450"
     CDS             complement(2198..>2537)
                     /locus_tag="VE308_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00255.1"
                     /translation="VFFLDVKVKDRSYHQKVLQRIYEELHEHIMLSSDMSNQEDSIGL
                     LLGSEDAIGIESIRSRSESFDGVHQANAFLPIKLACPQEWILKAIDKKLAESARKGKE
                     SSETVASRML"
BASE COUNT          637 a          610 c          527 g          763 t
ORIGIN      
        1 acatttcgat ctcttgcacc aatggccttc atgacaccta tctcccgggt tctctcagtg
       61 accgacgtat acatggtggt aactattccc acagagccaa ctaggagggc tactagcgca
      121 atgctcgaaa tgacgctgcc aaagccagca gtaaactcgc cgattgattg aattattgcc
      181 tttgaagttg ttatccctat gtcggatccg tagaggtctc gtatctcttt ttcaacctgc
      241 gtgacatggt ccgtgctttc tgctgctaca aagagagaat cgtacctacc agatttttga
      301 aggagctcgt tgcctgcatc gagattaaat actactgccc tgtcaattgt cgggtttccc
      361 actggcttca ttatcccggc cacggtgaat acttcagact gggattgccg gaatgcagta
      421 gccagatcga tgtaagtgta ggtaacgttt actcgctggc ctatcgttat tgcagcagtg
      481 gaaccatcat cgttgttcgc agctgcttct tctgacagct cacttaccca caggttattg
      541 actatcaaag acggaagata tattgctgat tcgtctttgg ggtctatagt cgatccttcc
      601 gtaaattcta gagtgggggc aatgatcttg aggttttcag gctgcaccga tagcacgctt
      661 gcactctgtg atctgttctc tacagcaatt gtgattcttg cctggtacga agggataacg
      721 gcgctgacac cggccagtga ctctagctta tccacaacag tttgatcaag ggttatcgca
      781 ggaacaggta ttaggccctg accaaggaat gtaggtggac cgtcaccgtt atccgcgtcg
      841 tctcgttgtc ccggaccgtc tataggaatg cttgtgacaa acagaatatt tggagcgagc
      901 ctgctaaaaa catctgttgc aaactggcca agccctgcca ctatgccgtt gagcgttacc
      961 attaggctac tgcccacaat caccatcagg attgtcaaag aagtcctgac ttttctatct
     1021 ccaagtgcct tgaatgaaat tctaaacgtt tgtatgactt tcatattttt taccttgctt
     1081 tttcttctga aatgatgtcc ttgttagcat cgtcatcatc agtaggcctt gcttcagttt
     1141 tcgatgtggt tttgtgcatt atttccttct ctattcttcc gtccctgatg tagatcgacc
     1201 tggtagtcat gcctgccagc tctagattat gtgtaaccat tacaactgta gtcccaaacc
     1261 tgtcagacaa catcttgaga agatcaaaca cctccctacc agtattggtg tcaaggtttc
     1321 cggtgggctc gtctgctaat actaaagctg ggtcgtttat cagggctctt gcaattgcca
     1381 ccctctgctg ttctcctcca ctcagatcta ctggtttttg ctttgccttg tccttgattc
     1441 ctagaatttc gagtaattcc agtgcccggg gatggctcct agcggaggag agtctctctg
     1501 agaatactgc tggaaactcg acattttctt gaacagacat tctgttgaca agattgtatg
     1561 actggaaaat gaatccaatc aaatggtttc tgacatcaga aagtcttgta tcgtcaagca
     1621 agaaaatatc ttgtccattt atgaacacct ttcctgaagt tggcctatca agagccccta
     1681 tgacgtttaa gagagtggat ttaccgcttc ccgatgggcc aactatcgag acgaactctc
     1741 ctccctttac ttcaaagctg acaccgttaa gggctaccac cattttggcc gaagctgagg
     1801 gtgaatcaaa tactttcttc acattttcca actttagcgc aacagcgaca gtagattgca
     1861 agtgggatgg aagatagcaa tggctttgct ttccagcggc ttcttcttct cttcttccgc
     1921 cagattcaga tacaaacttg gtattctcct catcgaccat catgttgcac acagggtctt
     1981 tgaacatata taccaactat agctgaattc ccttagataa acagagacct ttacaattgt
     2041 aaagctgcag ctgatatatt taattttaca attgtaaacc caggttgcta aataactatt
     2101 gatctaacta caagccatga cagcggagac gactaatcga caagagaact ggaataggat
     2161 caattactgc tgattaacaa aaccagatcg ttatggctta gagcatccta gatgcgacag
     2221 tttctgatga ttccttcccc ttccgtgcag actctgcaag ctttttatct atagccttga
     2281 ggatccattc ttgagggcaa gctagtttga taggcagaaa tgcgttggcc tgatgcaccc
     2341 catcgaagga ttcgctccta gatcgtatgg actcgattcc aattgcatct tcgcttccaa
     2401 gcaacaatcc tatgctatct tcttgatttg acatgtcaga cgatagcatg atatgctcat
     2461 gcaattcttc ataaattctc tgcagcactt tttgatgata gcttctatct tttaccttga
     2521 catctaaaaa gaacaca
//
LOCUS       DASQTF010000188         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1251126,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000188 DASQTF010000000
VERSION     DASQTF010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251126"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VE308_04455"
     CDS             <1..366
                     /locus_tag="VE308_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HZB00256.1"
                     /translation="AANLLESAGDYIVDLANAIDLSRIKVIDEIVEAGILVEKMHEKS
                     VAAFVNKNRSESNVVVKMYDNFMEIINSIKRSLDSKNLESTVAVLNLIYSMDKIARCW
                     VDVADLVKPTNLTAPLKNA"
     gene            376..1566
                     /locus_tag="VE308_04460"
     CDS             376..1566
                     /locus_tag="VE308_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZB00257.1"
                     /translation="MDNLVMARNNAIENVLILQGGGSLGAFGCGVFKAIAESSVRIDI
                     LAGTSIGGINAAIIAGSKDKRPEFALEQFWLELAENSIDLVPPMMSPIPLDPTVNELE
                     SHYSRYYQQAGVSLRQALSFYSSALYGNNMMFLPRWRPEYAAKDPKYFRPHEWTYIYD
                     HSPLASTLKKYINYDKLRPGGNPNTRLIITAVNVLTAEPMTFDSAHQQITVKHLLGTS
                     GYPLYGFPWVEVEKGTYVWDGSLLSNTPLREVIDVSPVIDKRIFIVENYPKKIERLPQ
                     NMPEVMHRARDIVFSDKTAHNIKMSKVITRHLEFIEELYKIVETNVEPSKIDRKKLDS
                     IHKKYQKMMKERGAEIKKIFYITREEQFPYLYENTDFTQTGIKNLIREGESKTRQALR
                     KIGL"
     gene            complement(1563..2552)
                     /gene="mvk"
                     /locus_tag="VE308_04465"
     CDS             complement(1563..2552)
                     /gene="mvk"
                     /locus_tag="VE308_04465"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HZB00258.1"
                     /translation="MSHGKASAPAKVILFGEHFVVYGSPAILAAINKRISVDASTLLH
                     DEDKIVIRSDIGVAGEYWHNGEFNALEGGRKAKAVLDPLYVAIKHVLLMRNKKNMGIE
                     VNISSRVPPGIGLGSSAASCVATVAAVDSLFQKKPSRQKVCELAIKSERQIHKRTSGA
                     DCYVSTFGGMMQYYSKSKSFKNIEEKGSLSLVVASTGIKHYTSDLVADVKRFKDTNKT
                     LFESLAKQASDICLQAWTAIESGRSDKIGELMNENQIILQQIGISHPKVHNIIDICSK
                     AGAIGAKITGAGGGGAVIALAASKHEGTKIASHVKAAGYQSFEVEIDYNGLSV"
     gene            complement(2549..>3039)
                     /gene="amrB"
                     /locus_tag="VE308_04470"
     CDS             complement(2549..>3039)
                     /gene="amrB"
                     /locus_tag="VE308_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZB00259.1"
                     /translation="RSAALDFDDYAHSRDHCLEVQLPFLQYIKQDFKIVPVVLVSQKR
                     DIALELGNAISQTIMEMDTLDSTLLLASSDFTHYEPNSEAHRKDGELIKTILALDINR
                     FYAVLERLNISACGYGAIATMMVAAKNLGATRGELLKYATSGDVTGDISAVVGYSSIV
                     FI"
BASE COUNT          863 a          683 c          663 g          830 t
ORIGIN      
        1 gcagccaatc ttctcgaaag cgccggggac tatatagttg atcttgcaaa tgcaatagat
       61 ctttcgcgca tcaaagtcat cgacgagata gtcgaggctg gaatacttgt agaaaagatg
      121 catgaaaagt cagttgcagc atttgtgaat aagaatagat cagaatcaaa tgtggtagtc
      181 aagatgtacg ataattttat ggaaattata aattccatca agcgcagctt agattcaaag
      241 aatctggaat ccacggtcgc agtactgaac ctgatatact cgatggataa gatagcgcgc
      301 tgctgggtcg atgttgcaga tctggttaaa cctacgaatc tcactgcccc tctcaaaaac
      361 gcataattac tgaaattgga taatttggtt atggctcgca acaatgccat tgagaatgtt
      421 ctgatattgc agggcggtgg gtcgcttggg gcctttggtt gcggagtatt taaggccatt
      481 gcagaaagca gcgtcaggat agacatcctc gctggcacat caataggggg aataaatgct
      541 gcaattatcg ccggcagcaa ggataagcgg cctgaatttg cacttgaaca attctggctc
      601 gagctcgcag aaaactcaat tgatctcgtg ccgccgatga tgtcgccaat tcctcttgat
      661 cctaccgtca atgagttaga aagtcactac agccgctact accagcaggc aggcgtatca
      721 ctcaggcaag cattgtcatt ctacagctcg gcgttatatg gtaataacat gatgtttttg
      781 ccacgatgga ggcctgaata tgcggcaaaa gatccgaaat attttcgacc ccatgagtgg
      841 acatacatct acgatcactc cccccttgcc tcgacactta aaaaatacat caactatgac
      901 aagttgcggc ctggaggaaa tccaaatact cgtctgataa taactgcggt aaacgtgcta
      961 actgcagagc caatgacatt tgacagcgca catcaacaga taacggtcaa gcatctgctt
     1021 ggcacttctg gatatcctct atacggcttt ccttgggttg aggtcgaaaa agggacgtat
     1081 gtatgggatg ggagcctgct gagcaacacg cccttgagag aagtaataga tgtttcacct
     1141 gttatagata agcgaatctt tatcgttgaa aactacccga aaaagattga aaggctgcct
     1201 cagaatatgc ctgaagtaat gcacagggca agagatattg tgttcagcga taagactgcc
     1261 cacaacatca agatgtcaaa agtgataacg cgacacctgg aattcattga ggaattgtac
     1321 aagatagttg agaccaatgt cgagccaagc aagatagaca ggaaaaagct ggacagcatt
     1381 cacaagaaat atcagaagat gatgaaagag cgtggagcag agataaagaa aatattttac
     1441 attacaagag aagagcaatt tccttatcta tatgagaata cggattttac gcaaacaggc
     1501 ataaaaaacc tcataagaga aggtgaatca aagacacgac aggcattgcg gaaaataggt
     1561 ctctaaacgg aaagtccatt atagtctatt tctacttcaa acgattgata gcctgctgcc
     1621 ttgacatgcg atgctatttt agtgccctcg tgcttgctcg ctgcaagagc aataactgcc
     1681 cctcctccac ctgcccctgt tatcttagct ccgatggcac cggctttgct gcatatatct
     1741 attatattat gtaccttggg gtgtgaaatg ccgatctgct gaagtatgat ttggttttca
     1801 ttcataagct cgcctatttt gtcagaccta cccgattcta tcgcagtcca tgcttgcaag
     1861 caaatgtcac tagcctgttt tgcaaggctt tcaaagagtg tcttatttgt gtccttgaac
     1921 ctcttgacat ctgccacaag gtctgaagta tagtgcttga ttccggtgct agcaactaca
     1981 agggaaagtg atcccttttc ctctatgttc ttgaagcttt ttgatttgct gtagtactgc
     2041 atcatcccac caaaggtact gacatagcaa tctgcaccag aagttctctt gtgaatctgt
     2101 ctctctgatt tgatcgcaag ctcgcatact ttctgcctgc tgggcttttt ttggaaaagc
     2161 gaatctacag cagccactgt cgcaacacat gatgcagccg aggatccaag gcctatgccg
     2221 ggaggtactc ttgaagaaat gttaacctct attcccatat tcttcttatt tctcattaat
     2281 agaacgtgtt tgatagcaac atatagagga tccaaaactg cctttgcttt ccttccacct
     2341 tcaagcgcat taaattctcc gttatgccag tattcgccag cgactccgat gtctgatcta
     2401 ataactatct tgtcttcatc atgcaataag gtgctggcat caacagatat gcgcttgttt
     2461 atagcagcaa gtatagccgg gctgccgtat acgacgaagt gctcgccaaa caggattacc
     2521 ttagccggtg cagacgcctt gccatggctc atatgaacac tatggaagag tatcccacta
     2581 ctgcgcttat gtctcctgta acgtctccgc ttgttgcata ttttagcagt tcccctcttg
     2641 ttgcaccaag gttcttagcg gctaccatca tcgtcgcaat cgccccgtag ccgcacgcag
     2701 aaatattgag cctctcaaga acagcgtaaa acctgttaat atcaagtgca agtattgtct
     2761 ttatcagctc tccatccttc ctatgtgctt cgctgtttgg ttcatagtgt gtaaagtccg
     2821 acgatgctaa tagcagggta gaatcaaggg tatccatttc cattatcgtc tgtgatattg
     2881 catttccaag ctcaagagct atatctctct tctgagagac gagtacaact ggcacaattt
     2941 tgaaatcttg cttgatgtat tgcagaaagg gcagttgaac ctccagacaa tggtctctac
     3001 tgtgggcata atcgtcaaag tcaagagcag ctgatcttc
//
LOCUS       DASQTF010000189         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_983192,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000189 DASQTF010000000
VERSION     DASQTF010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983192"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..299)
                     /locus_tag="VE308_04475"
     CDS             complement(27..299)
                     /locus_tag="VE308_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00260.1"
                     /translation="MNFVKGFEFVIIAMVAIFFLLVSVYIMMRQYKRLPKSYHIVVYD
                     VFGRETIIDGLRQTFATYDAAESYARLYREMYGRQQYEFKVIGNFL"
     gene            729..989
                     /locus_tag="VE308_04480"
     CDS             729..989
                     /locus_tag="VE308_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00261.1"
                     /translation="MSEIQPALLTRSELEWLLGKTSNLSKKTERDLRYRINKKVRIFM
                     QTELPLLKDRGFAAALGNDAAAISSGSGLGHCPGCTVRSSIA"
     gene            complement(1059..1164)
                     /locus_tag="VE308_04485"
     tRNA            complement(join(1059..1119,1150..1164))
                     /locus_tag="VE308_04485"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1096..1098),aa:Glu,seq:ttc)
     gene            1257..1919
                     /locus_tag="VE308_04490"
     CDS             1257..1919
                     /locus_tag="VE308_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HZB00262.1"
                     /translation="MANVVQRIDLEIEKRSLLKHSFYKMWSEGKLTIDHLQGYSKEYF
                     QLVKMVPKFVESIAEATGNPNIITNNACEEAEHVELWVRFATALGVSRSDLISYGGSE
                     KTNEAIAKLMALASLTFEEAVAAMYAYEMELPKISRSKIDGLKNLYGMDSQDATKYFE
                     IHEEADVRHAQVWRDILQRIPAERQEAALNAAIKSLQAQNMLLDSVNEKYVSGDGSRI
                     YC"
     gene            1980..2053
                     /locus_tag="VE308_04495"
     tRNA            1980..2053
                     /locus_tag="VE308_04495"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2014..2016,aa:Ile,seq:cat)
BASE COUNT          665 a          440 c          448 g          510 t
ORIGIN      
        1 caaacagagg atttcatatg gaaacactac aagaaattac caattacttt aaactcgtat
       61 tgctgtcgcc catacatctc acgatacaaa cgagcataac tttctgcagc atcatatgta
      121 gcaaatgttt gtctcagacc atcaatgatt gtttccctac cgaacacatc atatacaact
      181 atatgatatg actttggcag tcttttgtac tgcctcatca ttatgtatac actaactagc
      241 aggaagaata ttgcgaccat agcgattatg acaaattcaa accccttgac aaagttcaaa
      301 aaattggata tgagattctg atctagccat ctaaaagtga tactgaaggc cttatctgcc
      361 gcagatataa tgatgttaag catcattact ctctatcaat aaagagacat catattatat
      421 aataactgcg ttgaagcaat gcaagaactt taataatctg taagagcttg catgctatcc
      481 gcggcctata ataaaagaca gagactacct tgcttacaag gagtgaaatt gaacgacttt
      541 taggaaagcg ccaattgtcg aaacattata aactcaaaat gcgagctatt cttaataaaa
      601 gctcctgacc taggagcagt ctggcatcct aaagcacaag atagaagagc tgagaaaaat
      661 gccagttgag cagtttaccc ctctaaaaga ccaacactgc taatatattc aattatgtaa
      721 gttctcctat gtcagaaata caacctgcat tgctgactag gtcagaatta gaatggcttc
      781 tggggaaaac aagcaactta tcaaagaaga ccgaacgtga tttgcgctat cgaattaaca
      841 aaaaagttag gattttcatg cagactgaac ttccactgtt aaaggacaga gggtttgccg
      901 ccgctctagg taacgatgcc gccgcgatta gtagcggcag cggcttaggt cactgtcccg
      961 ggtgcacagt ccggtcaagc atagcatagc ggactcgaga gtctcaccta atgagacgaa
     1021 atcaaatcaa agagaaggag aaaaatatga taagaggatg ctccggccgg gatttgaacc
     1081 cgggatcagc gactcgaaag gccgctatgc ttgaccggac tgtgcagcca tatccataga
     1141 agacggctac tacaccaccg gagcattcgc aaactgtgta agaaccttcc cataaaatcc
     1201 tttcttgcta gatttcataa ggtttttcta ttggagagag aaatgagatc actgatatgg
     1261 ccaatgttgt gcagaggatc gacttagaaa tcgaaaagcg cagtctactc aagcactctt
     1321 tctacaagat gtggtcagag ggcaagctga caatcgatca tcttcagggt tattcaaagg
     1381 aatattttca gctggtgaag atggttccaa agtttgttga aagcattgcc gaggcgacag
     1441 gcaatcctaa tattatcact aataatgcct gcgaggaggc agaacacgtc gagttgtggg
     1501 taaggttcgc cacagcactt ggcgtttcaa gaagtgatct tatcagttat ggcggctcag
     1561 aaaagactaa tgaagctata gccaagttga tggcacttgc cagccttacg tttgaagaag
     1621 cggtagcagc aatgtatgcc tacgaaatgg agcttccaaa gataagcagg agcaaaatcg
     1681 atggattgaa aaatttgtac ggtatggaca gccaggacgc aacaaaatac tttgagatac
     1741 acgaagaagc agacgtgagg cacgcgcagg tatggagaga catcttacag aggatcccag
     1801 cagagagaca ggaagctgca ttaaacgcag ctataaaatc actgcaagcg cagaacatgc
     1861 tgcttgattc ggtcaatgaa aagtatgtca gcggagacgg cagtcgcatt tactgctagc
     1921 ttccaattct aattctccaa acctttatac atacttttac tcttcaattt tttctgtttg
     1981 ggcccatagc tcagcctggc agagcgaccg gctcataacc ggtcggccag gggttcaaac
     2041 ccccttgggc ccactccgat att
//
LOCUS       DASQTF010000190         8337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_250905,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000190 DASQTF010000000
VERSION     DASQTF010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250905"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VE308_04500"
     CDS             complement(<1..184)
                     /locus_tag="VE308_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZB00263.1"
                     /translation="MTDGETKSILDRKISFLGLAAIIATLATINFSGIAFAYAPGDPA
                     EVYSLWETDEAGNPVLD"
     gene            380..1249
                     /locus_tag="VE308_04505"
     CDS             380..1249
                     /locus_tag="VE308_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZB00264.1"
                     /translation="MAADINSLIQDWGSSVVTYITSSDDTDRLAEYTLHLARILAYPT
                     LDVSAILSSIDAMGQDVARLLKKSGNMPPRPTQIIEKINDHLFNIQKFKPNTDDYYNP
                     LNSYLNVVVERKRGIPITLCILYMRVARSVNFKMYPVNFPGHFLIKYILEDNSGEIIV
                     DPFNGGRIMDDYSLKALIDQAYPQQNIPLTHALVEKATAAQVVIRMLNNLKGSYYETQ
                     DMDRYNIANEMVLAIDRYNPDAVRDKGIVLLKKGDPSEALKVLNSYLEINPEAEDADD
                     VLDIIRQIRSGRK"
     gene            1253..1528
                     /locus_tag="VE308_04510"
     CDS             1253..1528
                     /locus_tag="VE308_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00265.1"
                     /translation="MPTIAKYIYIGAITLAASGAVAIYLNFQGIKCLSDLVQVDRSVL
                     TPERLEEMERTCSIITNSYVYSVYGITVGIILIVMGLVKKRKSMHAF"
     gene            1567..2169
                     /locus_tag="VE308_04515"
     CDS             1567..2169
                     /locus_tag="VE308_04515"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZB00266.1"
                     /translation="MAAQKHDDDSLEKIAAEVRGCPLCKLARTRKNAVPGEGQLSAKI
                     MFIGEAPGRSEDEKGRPFVGAAGRVLDDLLKKAGIERSQVFITNIVKCKPPNNRVPKE
                     DELTACRPYLDRQIALIKPKVICILGRTAYSSLLGGSSITANRGKILKRAGQKYFLTF
                     HPAAVIYNKGLLSVLEADLKKLAVEINKDEEREASLEDYL"
     gene            2166..2783
                     /locus_tag="VE308_04520"
     CDS             2166..2783
                     /locus_tag="VE308_04520"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZB00267.1"
                     /translation="MSCQNSSFYQRPTEVVAKDLVGKKLVRTIRNNKKQFRLTGLIVE
                     TEAYGYSDDLASHAYVGPTGRNKVMFGDVGRAYVYFTYGNHFCLNVSARRSKVEAGAV
                     LIRGIEPVEGVELMRQFRPVDDIFSLTSGPGKLTQALNITSSLNGADMTNSESEIYIE
                     FGKRPKHIVTTPRIGIARAVDKEWRFVDPSSPFISRKFQIKSSSL"
     gene            complement(2990..4078)
                     /locus_tag="VE308_04525"
     CDS             complement(2990..4078)
                     /locus_tag="VE308_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HZB00268.1"
                     /translation="MQDITASDRLAVKSFATKNPVFVCVISYTATSEIPGLTVAGANA
                     ELVKYTSPADAEFLYHGRCSCIDVVPATPDGKPTPALITRTALQLARIPLLIVDAGAK
                     VKPSIPYVSFGVEPGRNVMNENAMDISSVKRAFEYGELLGKQLAIMSDLTVVGESIPG
                     GTTTALAVLRALGIDARFKVSSSMPENPHNLKNKVVDSAIERVITEIGRISSPFEAIS
                     TLGDPMMPSIAGIASGVLSASGKVMLAGGTQMSAVVAILKHLGRTLNGLYIGTTTYVT
                     KDPSSDLFGLVRMASERVPILSCDLHLGESSKPGLQAFAKGFVKEGVGAGGASVVAIT
                     KSRGKITGKKLMNAIEKEYEVSIEAARH"
     gene            complement(4079..4705)
                     /locus_tag="VE308_04530"
     CDS             complement(4079..4705)
                     /locus_tag="VE308_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZB00269.1"
                     /translation="MKPAIMQIAAGQMANFTYIVADEENGEAAVIDPSWDLDKIFQML
                     TKNNWRAKYIINTHTHFDHVLGNEQMAEITGAKIVQHKNSQLKKDIAVSDGDTIEIGS
                     IRLQILHTPGHSKDSICLVLDDQFILTGDALFVGTCGRVDLPGSDAREMYDSLFDRLA
                     KLDEKLILYPGHNYGPTSISTIGHEKKTNYVLQPRSKQEFLELMTTGE"
     gene            complement(4702..5388)
                     /locus_tag="VE308_04535"
     CDS             complement(4702..5388)
                     /locus_tag="VE308_04535"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HZB00270.1"
                     /translation="MLLKPITSDQMYTIEQNGNSIFGMRRFLMMENAGHGVADFVVRK
                     FKNLKNKRIVTICGTGNNGGDGFVASRHLAGYGAKVVVILLGSPSDVRSEEARLNWSI
                     IEKMDSIELIFGKELNSEIKNRIANANIILDGIFGTGIKDEIRQPYASAIDTINKSKA
                     YVLALDVPSGFDPNTGQIHKKCILADATITFHRLKVGLAGRKKEKKKYTGSVYVEWIG
                     IPPEAERGIV"
     gene            complement(5759..6004)
                     /locus_tag="VE308_04540"
     CDS             complement(5759..6004)
                     /locus_tag="VE308_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00271.1"
                     /translation="MQAIRNFDGYPKLRTLRNMLPSLTPVQINVIVHHLERSGAIVID
                     ADGYITWIRNEDVEQLTLGEVADISSDLKELLKKHPK"
     gene            complement(6115..6450)
                     /locus_tag="VE308_04545"
     CDS             complement(6115..6450)
                     /locus_tag="VE308_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00272.1"
                     /translation="MSQNEIVYLKYQNLEGMLKVIIYSAQSMLGVIPMLYYIHYNSKH
                     VLFIQTGAVGGVTVHYVVENEKPTKRFIQLKRLSGEFTFVDSIGTDAQSLYVPILELE
                     KSTFIFPNL"
     gene            complement(6839..7189)
                     /locus_tag="VE308_04550"
     CDS             complement(6839..7189)
                     /locus_tag="VE308_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZB00273.1"
                     /translation="MVDDELDIVLIFKQALSRQGYTVFGFTDPLLALEHFKVNAEDYG
                     LVITDVRMPRMSGFELAANIKVIKPDAKVVFMSAFEVSDLEFSMPGVEINDFLRKPVD
                     VKTLVRRVKVAMAN"
     gene            complement(7758..>8337)
                     /locus_tag="VE308_04555"
     CDS             complement(7758..>8337)
                     /locus_tag="VE308_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZB00274.1"
                     /translation="TDAQAAIDSAKHALEQLRQASEFSKMLERIRSMIFNRDGMVGIS
                     LRSWALGVISQKASEYASLFNIGISRIELTEGAREVAIMCYGRQGEIDMDSLSGGEKV
                     AVALALRLSIAYMMGSNKLDFIILDEPTAHLDEDRRRGLVRILSEAFREGVGPLAQLI
                     IITHDSEIFENSEVDQIFRFVMTADGSCISRE"
BASE COUNT         2335 a         1870 c         1742 g         2390 t
ORIGIN      
        1 cgtctaatac agggttcccg gcctcatctg tttcccataa tgaatatacc tccgctgggt
       61 cgccaggcgc ataggcaaac gcaatgcctg agaaattaat ggtcgccagc gtagctatta
      121 tagcagctag acccagaaaa ctgatttttc tatcaagaat actcttcgtt tcaccgtctg
      181 tcatatagga gtgaaggctg gaaagtttga ataaaagcat ttatcttaat ttttattaac
      241 tgacgtatat tttggttcaa tataaaagtg aaaataataa cgtaaaagtc aaattttatg
      301 ataaggtgct ttattatcag atttgcaatc caaacttcta tgattgacat aaatatgtgc
      361 cagccacttg agaagacaat tggccgccga tatcaattcc ttaattcaag attggggaag
      421 tagcgtcgtc acttacatca catcatctga tgataccgat aggctggccg agtatactct
      481 gcatcttgca aggatacttg cttaccccac tctcgatgtc tctgcgatct tgtcctcgat
      541 agatgcgatg ggacaggacg tagcccggct acttaaaaag agcggcaata tgcccccgcg
      601 accaacccag ataatagaaa agataaatga ccatctcttt aacatacaaa aattcaagcc
      661 aaataccgat gactattaca atccacttaa tagctacctc aacgtagtag tcgaacgcaa
      721 aagagggatc cctattacac tttgcatact ttacatgaga gttgcccgct cggtgaactt
      781 caagatgtac cctgtgaact ttcccggcca ctttttgatc aagtatattc tcgaggacaa
      841 tagtggtgag atcatcgtcg atccgtttaa tgggggtagg ataatggacg actattcgct
      901 caaagcattg attgatcagg cgtatccaca acagaacatt cctcttactc atgcacttgt
      961 ggagaaagca acagcagccc aggttgtgat aaggatgttg aacaatctga aaggaagtta
     1021 ctacgaaaca caggacatgg acaggtacaa tattgcaaat gaaatggtgc ttgccattga
     1081 tcgctacaac cctgacgcag taagggacaa ggggatcgta cttcttaaaa agggcgatcc
     1141 ttcagaagca ttaaaagttc tgaactctta tctagagatc aatcccgagg ctgaggacgc
     1201 tgacgacgta cttgacatta tccgccagat caggtccgga aggaaatagg ttttgccgac
     1261 gattgcaaag tacatataca tcggagcaat aacactcgct gcaagtggag cggttgctat
     1321 ttaccttaac tttcagggca tcaagtgcct ctcagacctt gtgcaagttg acaggtctgt
     1381 tctaactcca gagcgcttag aagaaatgga gagaacctgc tcgatcatta ccaattcata
     1441 tgtctattct gtctatggca tcacagtcgg aattatcctt atagtaatgg ggttagtaaa
     1501 aaagaggaag agtatgcatg ctttttaaga tatatgttgt tggagaagat gatgatgtga
     1561 catcttatgg cggcacaaaa gcatgatgat gactcacttg agaagattgc cgctgaagta
     1621 agaggttgcc ctctctgcaa gctggcacgg acaaggaaga atgctgtgcc aggcgaaggt
     1681 cagctttctg ctaagataat gttcatagga gaggcaccag gccgtagcga agacgaaaag
     1741 ggcaggccat ttgtaggagc cgctggtcga gtccttgacg acctgctgaa aaaagcaggc
     1801 atagaaaggt cgcaggtgtt tatcacaaac atagttaagt gcaagcctcc aaacaacagg
     1861 gtaccaaaag aagacgagct gaccgcctgc agaccatatc ttgacaggca gattgcactg
     1921 atcaagccca aagtgatctg tatacttggc aggactgcat actcttcgct tttaggcggc
     1981 agttcgataa ctgcaaatag gggcaagata ttgaaaaggg ctggccaaaa gtactttttg
     2041 actttccacc cagcagctgt tatttacaat aaaggccttc tttctgtgct tgaagccgat
     2101 ctgaaaaagc tggctgtaga aattaacaag gacgaagagc gtgaagcgtc attggaggat
     2161 tatttatgag ctgccagaac agctcctttt accagcggcc tactgaagtc gtggcaaaag
     2221 atcttgttgg taagaagctt gtccgcacga taagaaataa caagaagcag tttcggctca
     2281 ctggcttaat cgtggagaca gaggcatatg gctatagtga tgacctagcg agccatgcat
     2341 atgttggccc aacaggcaga aataaagtta tgtttggcga cgttggaagg gcatacgtgt
     2401 atttcaccta cggcaaccac ttttgcctaa atgtctctgc aagaagaagc aaggtagagg
     2461 caggcgcagt cctgattcgg gggattgagc ctgtagaagg tgtcgaattg atgaggcagt
     2521 ttcggccagt cgacgacatt ttttcactca cctctggccc tggcaagctc acacaggctc
     2581 tcaatatcac atcatcgctc aatggagctg atatgaccaa ttctgaatcg gagatttata
     2641 tcgagtttgg taagaggccc aagcatattg tcactacgcc aaggatcggc atcgctagag
     2701 cggtggacaa ggaatggcgc tttgtcgacc cctcgagtcc ctttatatca cgcaaattcc
     2761 agatcaaatc atcatcatta tgactagtga tgatgggcta agctctatca atagtttcta
     2821 ttgttagatg attccgactg gatacaagac caaagtatct gtactattat ttttataaaa
     2881 gatgacatca aacttagagt caaactctag gtgctgctat atcaataaat ataatacaat
     2941 ttacctaaaa gctagtaact gtgaccccta tagaagtatt ggaatacagc tagtgcctcg
     3001 cggcctctat agaaacttcg tattcttttt caattgcatt cattaatttt ttacccgtta
     3061 ttttacctct tgattttgtg atagccacaa ctgaagcgcc gcctgcaccg acgccttcct
     3121 ttacgaatcc ctttgcaaat gcctgcaggc ctggcttgct cgattcgcct agatgaagat
     3181 cgcaagataa aattggaact cgctccgaag ccattcttac aaggccaaac agatccgaag
     3241 acggatcttt tgtgacatat gttgtagtgc caatataaag accgtttagc gtcctgccga
     3301 ggtgcttcaa tattgcaacg actgcagaca tctgagtgcc tccggcgagc ataaccttgc
     3361 cacttgcaga cagcacaccg cttgcaatgc cagctataga aggcatcatt ggatctccaa
     3421 gagtggatat agcctcaaag ggcgagctga tccttccaat ctcagtaatg actctctcta
     3481 ttgcagaatc tactaccttg tttttcagat tgtgcggatt ttctggcata ctactgctta
     3541 ccttgaatct tgcatcaatg ccaagtgctc tcagcacggc aagtgcagtt gtagtgcctc
     3601 caggaatgct ctcgcctact actgttagat ctgacataat ggccagttgt tttccaagta
     3661 gctcaccgta ctcaaacgca cgtttgacgc ttgagatgtc cattgcattt tcgttcatga
     3721 cgtttctacc cggttcaact ccaaacgata catatggtat tgagggcttg acttttgccc
     3781 ctgcatcaac tatcagaagg gggatccttg ccaattgtaa cgcagtgcgt gtaatgagtg
     3841 ccggcgtagg tttgccatca ggtgtagccg gtacaacatc aatgcagctg cagcgtccat
     3901 gatacagaaa ttccgcgtct gccggcgatg tatatttcac tagttcggca tttgcccctg
     3961 ctactgtaag ccccggaatc tcgcttgtgg cagtgtacga gatcacacac acaaaaacag
     4021 gatttttcgt cgcaaaactt ttgactgcaa gcctgtcaga cgctgttatg tcctgcatct
     4081 attcaccagt ggtcataagc tccaagaatt cttgttttga cctaggctgg agcacatagt
     4141 tggttttctt ttcatgtcct atcgtagata tagaagtcgg accatagttg tgaccgggat
     4201 ataagatgag cttttcgtct agttttgcca gcctatcaaa taggctatcg tacatctctc
     4261 ttgcgtcgct tccaggaaga tccactctgc cgcaggtgcc cacgaagagt gcgtcaccag
     4321 ttagaataaa ttgatcatca agcacgaggc aaatgctgtc cttagagtga cccggtgtgt
     4381 gcaaaatctg tagcctgata ctccctattt cgatcgtatc tccgtcagat actgcaatgt
     4441 cttttttcag ctgcgagttt ttgtgctgta ctatcttagc accagttatc tctgccatct
     4501 gctcgttccc aagtacatga tcgaaatgag tatgagtatt gatgatatac ttggccctcc
     4561 agttgttctt tgttagcatc tggaatattt tatccaagtc ccatgaagga tcaatgactg
     4621 ccgcttcgcc attttcctca tcagctacta tgtaggtaaa attggccatc tggcctgctg
     4681 cgatctgcat tattgctggc ttcatacgat gcccctttca gcctccggtg gtatccctat
     4741 ccattccaca tatacagaac ctgtgtattt ctttttctct ttcttcctcc ctgcaagtcc
     4801 aacttttaat cgatgaaagg taatagtggc atctgccagg atgcactttt tatggatctg
     4861 cccagtgttt gggtcaaatc ctgaaggaac atcgagtgcc aagacataag ccttagattt
     4921 gtttattgta tctattgcag atgcataggg ctgcctgatc tcatccttga tgcctgtgcc
     4981 aaatatacca tctaagataa tgttagcatt ggcgattcgg ttcttaatct cgctgtttaa
     5041 ctccttgcca aatattaatt caattgaatc cattttttcg attatgctcc agttaagcct
     5101 tgcctcttca cttcttacat cagaagggct cccaagtaaa ataactacta ctttggctcc
     5161 atacccagca agatgacgcg atgcaacgaa cccatcgcca ccattgttgc cggtgccgca
     5221 tatcgtaact attcttttat tcttcaagtt cttgaacttt cggacaacaa aatctgctac
     5281 tccgtggccg gcgttctcca tcatgagaaa acgtcgcatg ccaaaaatgg aattgccgtt
     5341 ctgttcaatg gtgtacatct gatctgaagt aatcggcttc aataacacat gtgacggtct
     5401 attcagacaa acataaaaac tatttaacaa aaatttggac gtcttcgata agaaccctcc
     5461 tcagttcatt ctaagctctc ttgaaccaag accccactta cttgttgtag tttagtcctt
     5521 ttcttcttat tctataaggc agtttttaaa cagtgttgat taatattcgt tttagctgtg
     5581 agcatgagag agaataaacc ctcgtacatt gctttggttt ggttgcacca ctgtttgcag
     5641 cctaaataat attgcaacaa tcaacaatat ctgtcctcta ttgtattctt caggacacct
     5701 gaatcagaga tatctaaact taacataaga tctgagagac gtcaaagtca aatcctctct
     5761 atttgggatg cttcttgagc agttccttga ggtcactgct tatatcggct acctcgccaa
     5821 gcgtcagctg ttccacatct tcattacgga tccacgtaat gtatccatca gcgtcaataa
     5881 ctattgcccc cgacctctcc aggtggtgca ctataacgtt tatctggact ggtgttaggg
     5941 atggaagcat gttgcgaagg gttcttagct tcggatagcc atcaaaattc ctgatcgctt
     6001 gcaaaaaagc gttaatgtca tcctctgaaa cttcatttgc cggtcttgcc tttctagagg
     6061 attttcgggg tttgatagaa aatgtatgtc gccttgccct aaaacttttt ctccctatag
     6121 gtttggaaag ataaaagtag atttttccag ctccaatatc ggaacataca gtgactgtgc
     6181 atcggtgcct atgctatcta cgaatgtgaa ttctccagaa agccttttta gctgaatgaa
     6241 ccttttggtc ggcttttcat tctcaacaac atagtgaact gtcacgcctc ccactgcacc
     6301 tgtctgaata aaaaggacat gcttgctgtt gtagtgaata taatagagca ttggaatgac
     6361 tccaagcatc gactgagcgg agtatattat caccttgagc atgccttcta ggttctggta
     6421 ttttagatac acgatctcgt tttgtgacat ggtattaaca taaatctcac atcatatttt
     6481 ctttttgatc attgcaaatt tgctttgtgg tcggtgaagc gtgatgtata ctctctgatg
     6541 gtgtggtagc ctatagagcg cctgcattca tgagcacaga tatatataca ggcaaacccg
     6601 accttggaga accggtttgt aacaagtcct tctagaatgt atagacctgg tgctcaagtg
     6661 ggtttgctga cttggttaga attctcagac actttttata tatagaatag aggtatatgt
     6721 acaatttctt ccacgttgca agattgataa agaccgtata agacaagcaa gccggatgct
     6781 tttgaaaaca aatacagcct tgagcagctg cagatatgtc tgcaagtaat agataatttt
     6841 agtttgccat agccaccttg actcttcgca caagggtctt gacatcaacc ggctttcgga
     6901 ggaagtcatt tatctcgact cctggcattg aaaattcaag gtcacttact tcaaatgccg
     6961 acatgaacac tacttttgca tccggcttga taactttaat gtttgcagca agctcaaagc
     7021 cgctcatccg cggcattctg acatctgtta ttaccaggcc ataatcttcg gcgtttacct
     7081 taaaatgttc aagagccagc aaaggatcag taaagccaaa gacggtatag ccctgcctac
     7141 tcaatgcctg cttgaagatc aacactatat ccaactcgtc atcaaccacc agtatggaat
     7201 gagcagaaga caacagtcta ttgccacctc gcttcgatgc gatacacttc actattagca
     7261 aggtggtgcg atgacattaa agccatagtt gagaacaaga cacgaactta tattgtctgc
     7321 gaaaaaatat tctatagaat gcatgtctag gaaaatcaat ttttgaaata tcgatctatc
     7381 cacatcagtg atgatgacct agtgagtgct cgaacttctt gcttttgcaa gacaccattc
     7441 tagcataatt aaagcatagt atgtatacaa gctcactact taaagttaca agaacggcga
     7501 tgggcgatta ttatccatga ttctcgtaat aactgctttg agattctggc aggtgataaa
     7561 aaaggctcaa catggtgcag ttcgcccaag acacaaaaga tggcagattg tcaaaaaaat
     7621 aataactata tctagaaagc agttcatcaa ctgatgaaat cgtcactgat attagcattt
     7681 tgaaaaaatt tctattgatg agtttgaaaa aacagagata ttatgatgtt ggaagatgac
     7741 tacaaaagat gactttctta ttctcttgag atgcatgaac catcagctgt catgacaaac
     7801 ctaaatattt gatcgacttc agagttttca aaaatttctg agtcgtgagt tattattatc
     7861 agctgagcca gtggtccgac accctctctg aatgcctcag atagtattct gacaagcccc
     7921 ctcctcctat cttcgtcaag atgtgcggtc ggctcatcta aaatgatgaa atcaagcttg
     7981 tttgaaccca tcatatatgc aatactaaga cgaagtgcaa gggctacggc caccttttca
     8041 cctcctgaaa gtgagtccat atcaatttca ccctgtctgc cgtaacacat tatcgctact
     8101 tcccgtgctc cttctgtaag ttcgatcctc gaaatgccaa tgttgaagag cgaggcatat
     8161 tctgacgctt tctgcgatat aactccgagc gcccatgagc gaaggctaat acccaccatc
     8221 ccgtccctat tgaatatcat cgagcgtatc ctttccagca ttttggagaa ttcagatgcc
     8281 tgtcttaatt gctccaatgc atgcttggca gaatctatgg ctgcctgggc gtcagtt
//
LOCUS       DASQTF010000191         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_986607,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000191 DASQTF010000000
VERSION     DASQTF010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986607"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VE308_04560"
     CDS             <1..297
                     /locus_tag="VE308_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00275.1"
                     /translation="LVEVHTEQEFGEVVKAKHDLVGINNRNLDNLQVDITNTEKLLKR
                     YDKGTNLIISESGIVSPEDIQYLKSAGADAFLVGTSIMQTPDIGTKISELYYAL"
     gene            346..1572
                     /gene="trpB"
                     /locus_tag="VE308_04565"
     CDS             346..1572
                     /gene="trpB"
                     /locus_tag="VE308_04565"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZB00276.1"
                     /translation="MLGSYPVGGKFGRYGGKYIPETLAPAIEELESAYEKYKNDPDFQ
                     NELSYYLTEYAGRPTPLYFAKNLTKKLGGAKIYLKREDLLHGGAHKTNNTLGQALLAK
                     KMGKKRIIAETGAGQHGVGTAIACAVLGLSAEIFMGAKDFQRQKLNVFRMELLGAKVY
                     PVQSGSKTLKDAINEAIRDWIANVETTYYLIGSAVGPHPYPMMVRDFQNVIGREIKQQ
                     IREIEGTMPNAVVACVGGGSNAIGSFYTFLDNKDVVLYGIEAGGRGIKSGKHSATLAA
                     GSEGILHGMFTCLLQDECGQVVETHSISAGLDYPGVGPEHAMLKDVKRARYASSTDQE
                     AIEAFLALSRFEGIIPALEPSHAISYAMKLAQQMKKDDIIVVTLSGRGDKDVEIVQDY
                     LNKNKYNSKKKTKHAK"
     gene            1562..>2118
                     /gene="trpA"
                     /locus_tag="VE308_04570"
     CDS             1562..>2118
                     /gene="trpA"
                     /locus_tag="VE308_04570"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZB00277.1"
                     /translation="MQNRITEKFCELAKKGESALICYVVAGYPDIGTCKQVINALVSG
                     GADIIEVGIPFSDPIADGPTIQAASNTALRNGMTPEKALNLVRSVRKQHPNLPLLAMT
                     YSNILVRAGMRKFMSESKQSGLDGFILPDMPVEEADTYSLMASKLNLATVFLVSPNTP
                     DERLRKIVASTTGFVYVVSVYGITGA"
BASE COUNT          671 a          442 c          504 g          501 t
ORIGIN      
        1 ctagttgaag ttcataccga acaagaattt ggagaagtgg tcaaagcaaa acacgattta
       61 gtcggcatca acaaccgcaa tcttgacaac ctacaggtag acattacgaa cactgaaaaa
      121 ctgctgaaaa ggtatgacaa gggtacgaat ctgataataa gtgagagcgg catcgtgagt
      181 ccggaagaca tacaatactt gaagagtgca ggagcagacg cctttttggt aggaacaagt
      241 ataatgcaaa cacccgatat tggtaccaag atctccgaat tgtattatgc attatagaat
      301 cagacatccc ttaaaatacc cccttgaaca ctacatgatt catctttgtt aggaagttat
      361 cccgtaggtg gcaaatttgg aaggtatggc ggtaaataca tacccgaaac gttagcgcca
      421 gcaatcgagg agctagaatc cgcctatgag aaatacaaga acgaccctga ctttcagaat
      481 gaactttctt attatctcac cgaatatgct ggcaggccta ccccacttta ctttgctaag
      541 aacctaacaa aaaagttagg cggcgccaag atatacctaa agcgcgaaga tcttttacat
      601 ggtggggccc acaagaccaa taacacgctt gggcaggcgc tcttggcaaa aaaaatgggc
      661 aaaaaaagga taattgctga aactggtgct ggccaacacg gtgttggcac agccatagcg
      721 tgcgcagtgc ttggactttc tgcagaaata ttcatgggag ctaaggactt tcagcgacaa
      781 aagctaaacg tctttaggat ggagcttttg ggagcaaagg tttatcctgt ccaatccggg
      841 agcaagacac taaaagacgc aataaatgag gcaatccgag actggatagc caacgtagaa
      901 acgacatact acctcattgg ctcggcagta ggtcctcatc cttacccgat gatggtgagg
      961 gactttcaaa atgtgattgg gagagaaata aaacaacaga ttcgggaaat cgaaggtact
     1021 atgcccaatg ctgttgttgc atgtgttggc ggaggaagca acgcaatagg ctcattctat
     1081 acattccttg ataacaagga cgttgtgcta tatggcatag aagcaggagg tagaggaatc
     1141 aagtcaggca agcactcagc tactcttgcc gctggttcag aaggcatctt gcacggcatg
     1201 ttcacctgcc tgttgcagga tgagtgtgga caagttgttg aaacccacag tatatctgca
     1261 ggacttgatt atcctggtgt aggacccgag catgcaatgc tcaaagatgt caaacgcgca
     1321 aggtatgctt catctacaga ccaggaggct atagaagcat tccttgcatt gtctagattt
     1381 gaagggatca tccctgctct ggagccgtca catgcgattt catatgcaat gaagcttgcg
     1441 caacagatga aaaaggatga cattatagta gtcactcttt ctggtcgagg agacaaagat
     1501 gtggaaatag ttcaagatta tctcaacaag aacaagtaca atagcaagaa aaagacaaag
     1561 catgcaaaat agaatcactg agaaattctg tgagctggca aaaaaaggcg aaagcgctct
     1621 tatttgctat gtagtggcag ggtacccaga cattggaact tgtaagcagg taattaacgc
     1681 attggtgagt ggcggggccg acattatcga ggtaggcata cctttttcag atccgatagc
     1741 ggatgggccc actatacaag cagcttcaaa cactgcgtta aggaatggca tgacaccaga
     1801 aaaggctctt aatctggtaa gaagtgtcag aaagcaacac ccaaatctac ccttgcttgc
     1861 aatgacctat tcaaacatcc ttgtccgagc aggcatgaga aaattcatgt ctgagtctaa
     1921 gcaatctgga cttgacgggt ttatattgcc agacatgcca gttgaggagg cagacacata
     1981 ttcgctaatg gcttcaaagc ttaaccttgc tactgtattc cttgtatctc caaatacacc
     2041 agacgaaagg ctgcgcaaaa ttgttgccag cactacgggc ttcgtatatg ttgtatcagt
     2101 ttacggtata actggtgc
//
LOCUS       DASQTF010000192         6772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1091330,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000192 DASQTF010000000
VERSION     DASQTF010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091330"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VE308_04575"
     CDS             <1..422
                     /locus_tag="VE308_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00278.1"
                     /translation="IVGAITIDVVLTIIFVKIIGKLGIERLGIYGFSNVKTNAAFLAS
                     AAIGSLSHVFVDWLHHPANPIFWPFLVDGSYYVNGLLLSFMAVLPASMIVTTISGAIM
                     AAVIARALDKSGYSFWLVFSNPAKALSFITESLCKAN"
     gene            complement(415..1176)
                     /locus_tag="VE308_04580"
     CDS             complement(415..1176)
                     /locus_tag="VE308_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZB00279.1"
                     /translation="MQVESDIPTETTTTTTLKEETPREDFAAEKQMISGKPVAAAQIL
                     VEEEDDDSQDSLSATDFHNNDNKILALLNEDGSNYSFKGLMRKLNLHQQSLSRALHRL
                     EEMELVEKSSIGYRLSKAGATAMSRIELLHQQHKPKGREYMQLLQTYIPLNIKASEVM
                     RHLIGRWFKNLRWIGMIRSDTGYTLQWTSDDGAFQINLRLIADYIVIETNASTEKEKV
                     QAMVGSYIIYEQITKVMQSRLDAYVLDHRDTSIIN"
     gene            complement(1245..1532)
                     /locus_tag="VE308_04585"
     CDS             complement(1245..1532)
                     /locus_tag="VE308_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="in yeast this protein is involved in start site
                     selection during the initiation of translation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HZB00280.1"
                     /translation="MSDYDSMDELIKEIEKEEAAIVITKDIRKYRKPVTVISGLQERK
                     DLKDITKQLKTKIGTGGTFKEGQIILQGDHRETAKSLLAQMGFKEEAIEVY"
     gene            complement(1584..2519)
                     /locus_tag="VE308_04590"
     CDS             complement(1584..2519)
                     /locus_tag="VE308_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZB00281.1"
                     /translation="MIPVPEDVLISAVEKVSKSVVNIASVRMLQDQMFRIFPVEGVGS
                     GVVIDEKGYILTNNHVIDDAERLKVTLPDGKVLRGKVVGSDETTDLAVLKVESEQSLP
                     ATQLGNSDNLKTGQIVIAIGNPFGLTGGPAVSAGIVSALNRSIQTRSGVLELIQTDAA
                     INPGNSGGPLVNTNGEVIAINTANMPYAQGIGFAVPINTAKSILKELIEKGKVTRPWI
                     GVASIKVTPQLARYYGLPVTDGALVARVEPYSPADDAGLRKGDIIEEIDGNMIKDPSQ
                     IATYVRKKQVDDQLTVTINRYGRQFQLPVPIDERP"
     gene            complement(2953..3543)
                     /locus_tag="VE308_04595"
     CDS             complement(2953..3543)
                     /locus_tag="VE308_04595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZB00282.1"
                     /translation="MKNTFNVLGVAGSTRHGSYSTRALKIALEHVKKHGAEVRLLDLN
                     RIVLPLYDPSASASKEVEQAAEAIAWADAFILASPDYHGSISGALKNFLDHFYEEFAG
                     KVFGYIVASHEKGLTVMDQMRTAVRQCYGWSMPYGVSINGPQDFTGHETANARLSSRL
                     QMMARDLVVYGRLVRDQFMLDFSSNEPDTFAAHYRP"
     gene            complement(3925..4467)
                     /locus_tag="VE308_04600"
     CDS             complement(3925..4467)
                     /locus_tag="VE308_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HZB00283.1"
                     /translation="MTRRGLMLGRFQPFHKGHLALAKQILSECDELVIIIGSAQFNFI
                     DKDPFSAGERVLMIHEALKEAGIDLSRCYIIPVVNDENNARWLAYLRSMIPPFDVLYS
                     GNDFVKYLARSQDPGIAIEDPAFAEKDEYNGTNIRHLMQGGKPWEHLVPPAVAKVIQQ
                     VGGIARINMLACSDSNPQRW"
     gene            complement(4464..6131)
                     /gene="metG"
                     /locus_tag="VE308_04605"
     CDS             complement(4464..6131)
                     /gene="metG"
                     /locus_tag="VE308_04605"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZB00284.1"
                     /translation="MPYPSSSENAIITSALPYANGEIHLGHIASTYLPADIFTRFLRL
                     KGLKAYHICATDDFGTPILIRAEKEKKSPKDYVEYWNRQDKEDFGSLGISFDFFFKTS
                     SKENVEFVQYVFKQLCENGHIYDQNVIQFYCTYDNKFLPDRYVLGRCPYCNAENQYSD
                     LCEKCGRVPDQILDPKCAICGRPPVKKESKHYFFRLSSLEVKLKEWLLSNQHLQPDVK
                     NYVINWINEGLQDWDITRDLSWGVPIPLPEAKGKVFYGWFDNHLCYISSLAKFVKDRG
                     GNGKKFWNESEIYHFIGKDIIYHHYLFLPAIRLGINSEYKLPDYIPTRGHLMLQNQKI
                     SKSRNWYIGLKDFTSAFNPDYLRFYIASVVTYSQDDLNFDFDAFYERINNELIANIGN
                     FINRALSFTQKSFQGIVPEPSNIDDKIEKELAATVDETGRLLSSNEIDKSLKRILKYS
                     NYMNHYFQTNSPWANKDTSNTTVYVSVNAVRSLAVLLEPFIPFSCEKIWAQLKLGGGS
                     VHQQNWDSAKDIRAIPAGHELGKVEPIFRKIEAKEIEQWKNKLGSPQ"
     gene            complement(6163..>6772)
                     /locus_tag="VE308_04610"
     CDS             complement(6163..>6772)
                     /locus_tag="VE308_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZB00285.1"
                     /translation="CGYGWVGKGVATRAKGMGAIVTVTEVDPIKAIEAKMDGFEVMRM
                     SDAAPAGDVFITCTGQTCVIRKEHFLKMKDAVILANAGHFDVEIDTKSLYDISCCQPV
                     EISPNIERFEVNGKRLFLLSKGRVVNLVAAEGHPPEVMALSFANQLLSILYIAKNHDR
                     MEVKVHSVPEQIDQDIGSYALDSMGIKIDQMIEEQERYQHSS"
BASE COUNT         1657 a         1620 c         1416 g         2079 t
ORIGIN      
        1 caattgtagg agccataacc atagacgttg tccttacgat aatttttgtc aaaattatag
       61 gcaagcttgg tattgaaaga ctggggatat atggatttag caatgtcaaa acaaatgctg
      121 cctttcttgc gtctgctgcg attggcagcc tgtcccatgt tttcgtcgac tggctccacc
      181 accctgcaaa tccgattttc tggccctttt tggtagacgg ctcgtattac gtaaatggac
      241 tgctgctttc gtttatggcc gttttacctg cctccatgat cgtgacaact atatctggtg
      301 cgatcatggc ggcagtcata gcaagagctc ttgacaagag tggctatagc ttctggctgg
      361 tgttttcaaa tccggcaaag gcactatctt tcataacgga atcactttgt aaagctaatt
      421 gattatacta gtgtctcggt gatccagtac atatgcatca agcctgctct gcatcacctt
      481 tgttatctgc tcatagatga tatacgatcc caccatcgcc tgcacctttt ctttttcagt
      541 agatgcattt gtctcgatga caatatagtc tgcaatcaat cgaaggttga tttggaacgc
      601 gccgtcgtcg cttgtccact gcagcgtata acctgtatca ctccttatca tgcctatcca
      661 ccgaaggttc ttaaaccacc tcccgatcaa gtggcgcata acttctgagg ccttgatgtt
      721 gagcgggata tacgtctgca ataactgcat atactctctg ccctttggtt tgtgttgttg
      781 gtgcaacaac tcgatccttg acattgcagt tgcaccggcc ttgctaagcc tataacctat
      841 cgaagacttt tccacaagct ccatttcttc tagcctatgg agcgctcttg acaagctttg
      901 ctggtggaga ttgagcttac gcattaagcc tttaaacgag taatttgatc catcttcatt
      961 cagcaatgcc aagatcttgt tatcgttgtt atgaaaatcg gttgcactaa gcgagtcctg
     1021 cgagtcgtca tcttcttctt ctactaggat ctgagcagct gccaccggct tgccactgat
     1081 catctgcttt tccgctgcaa agtcctctct aggggtctcc tccttgagag tagttgttgt
     1141 tgttgtttca gtcggaatgt cgctttccac ttgcatagta caagcgcccc ttcatgatat
     1201 aaattataaa gtaaccatct ggagagcagg taagtaaaat gctactagta cacctcaata
     1261 gcctcttcct tgaagcccat ctgagccagt agacttttag ctgtctctcg atgatctcct
     1321 tgaaggatta tctggccttc cttaaacgtg cctcctgtgc caatcttagt ctttagctgc
     1381 tttgtaatat ccttcaaatc ctttctctcc tgaagccctg atatcacggt gacaggcttc
     1441 ctatactttc tgatatcttt ggtaatcact atggctgcct cttccttttc aatctccttg
     1501 atcagctcgt ccattgaatc atagtcagac actgaaatga ttattaccac tcacgctcaa
     1561 aagtgttttc atataaaaaa ttatcatggg cgctcgtcta tcggaacggg gagttgaaat
     1621 tggcgcccat agcgattgat agtcaccgtt agttggtcat ctacctgctt tttgcgaacg
     1681 tatgtagcga tctgtgaagg gtcttttatc atgttgccat ctatttcctc gatgatatct
     1741 ccttttctta gtcctgcatc gtcagctggg ctataaggct caactcttgc aacgagcgct
     1801 ccatcagtca cagggagccc gtagtatcgt gcaagctggg gggtaacctt tattgatgca
     1861 acgccaatcc acggccgcgt taccttaccc ttttctatca attctttcaa tatcgacttg
     1921 gcagtgttga ttggaactgc aaatcctata ccttgtgcat aaggcatgtt ggcagtgttg
     1981 atagcaatca cttctccgtt tgtattgaca agcggtccac ctgagttgcc tgggttgatg
     2041 gcagcatctg tctgtattag ctctagcacg ccgctccttg tttgtatgct tctattcaat
     2101 gcgctgacta tgcctgcgct tactgcaggt cctccagtga gcccaaaagg gttgccaatt
     2161 gcaatcacta tctgacctgt ctttaggttg tctgagttgc caagctgtgt agctggaagc
     2221 gactgttctg attctacttt gagcactgca aggtcagtgg tctcgtcgct tccgaccacc
     2281 tttcctcgga gcactttgcc gtcaggtagt gtcaccttga gccgttctgc gtcgtcaatt
     2341 acgtggttgt ttgtaaggat atatcccttc tcatctatga ctaccccaga acctactcct
     2401 tctactggaa atattctaaa catctggtct tgtagcatcc tcacacttgc aatgttgaca
     2461 acactcttgc tcactttctc aactgcgctg ataagtacat cttctggaac aggtatcatg
     2521 ccaatatatt ggaggacagt aggtaaattg accggctatt ctagaattca gtcacactcg
     2581 ttgaacaaga gtaaaaaagt gaatttgaaa tggcaagaaa atgatattgt ggcaggctaa
     2641 gctaatactt cagttagaaa acattcaatt gcattcctgc aatggataat gcgagtaatg
     2701 aagcaaccac tcaattattg attaccaagt cttgcaaagg gtaattgaca gattcaaagc
     2761 actaatggaa tggataacat cgtaaactat ggcgaggcag gcaggccaat agtgctcaaa
     2821 tttgaagtat tttcatgcag tgaagaaaaa agacaaccac tggtaatacg gcatcgtttt
     2881 atcaggttaa attagctatg ctacactaaa aggcgaagat gaaatgcctt gggcttgcct
     2941 gaaccatctt ctttatggcc tgtaatgcgc tgcgaatgta tccggctcat tagagctaaa
     3001 gtcgagcata aattgatccc tgacaagcct gccatatacc accaagtcgc gtgccatcat
     3061 ctgcagtctg cttgacaatc ttgcattcgc tgtttcatgc cctgtaaagt cctgtggacc
     3121 atttatcgac actccatagg gcatactcca tccatagcac tggcgcaccg ctgtgcgcat
     3181 ttgatccata acggtaagcc ctttttcgtg tgatgcaact atgtacccaa ataccttgcc
     3241 tgcaaactct tcataaaagt gatcaagaaa gttcttaaga gcgccggata tcgagccgtg
     3301 gtagtctgga gatgcaagga taaaggcgtc agcccaagct atggcctcgg ctgcctgttc
     3361 tacctctttt gacgctgagg cacttggatc gtataggggc aaaactattc tattgaggtc
     3421 tagtaggcga acttctgcac catgcttctt gacatgctca agcgcaatct tgagtgcccg
     3481 cgtgctatat gatccatggc gagtgctgcc cgcaactcct aacacgttaa acgtattttt
     3541 catctcttat tattattgaa cttagccgga ttaatcgact tgcttttaca ggatctggtc
     3601 acatcatcaa ttcttgtgat tgcatattca tatggcatgt tccgcctgca taacagcaat
     3661 cgagggtaat agagcctttc gctatcacta acttgcatcc gactgatagc ccgtattcca
     3721 atctatatgt gatcatgatg atgatgatga tgctgttgtt gttgcccttc gttattgctt
     3781 tcttgaacta aacggtgatg cgcttggcgc atgatagaca ttcaacaatt acctgacttt
     3841 tgctcttatt tatcactttg aacttctttg aaccacatga caggcagacg gggcatgccc
     3901 tatgaccctt taccatgtct tctattacca cctctgtggg ttgctatccg agcaggccag
     3961 catatttatc cttgcaatgc ctccaacctg ctgtattacc ttcgcaactg ccggtggtac
     4021 tagatgctcc catggcttac cgccctgcat gagatgccga atgtttgtgc cattgtactc
     4081 atctttttct gcaaacgcag ggtcctcaat tgcaatgccc gggtcttgag agcgcgcaag
     4141 gtactttaca aagtcattgc ctgagtatag cacatcaaat ggtggaatca ttgaccgtag
     4201 gtatgcaagc catcgagcat tgttctcatc attcacaaca ggtataatat agcatcttga
     4261 gagatcaatt cctgcttctt tcaatgcctc atgaatcatt agtaccctct cgccggcact
     4321 gaaaggatcc ttgtcgataa agttgaactg cgcgctccct attattataa cgagctcgtc
     4381 acactcactt aggatctgct ttgcaagcgc taagtgacct ttatggaagg gctgaaagcg
     4441 tcccagcatg agtccacgtc tggtcattgt gggctgccta acttattctt ccactgttct
     4501 atttcctttg cttctatctt gcggaagatt ggctctacct tgccaagttc atggcctgca
     4561 ggaattgctc tgatgtcctt agccgagtcc caattctgct ggtgaacact gccacctcca
     4621 agcttgagct gggcccatat cttttcacat gagaagggaa tgaatggttc aagcaggacc
     4681 gccaagcttc gtacggcatt caccgatacg tataccgttg tatttgaagt atccttgttt
     4741 gcccaaggtg agtttgtctg gaaataatgg ttcatataat tactatattt taggatcctc
     4801 ttgagcgact tgtctatctc gttgcttgac agcagtctgc ctgtttcatc cactgttgcc
     4861 gcaagctctt tttctatttt atcgtcaatg ttggatggct cgggcactat accttggaat
     4921 gacttttgcg taaatgaaag ggctctattt atgaaattgc cgatgttggc gatcagctca
     4981 ttgtttatcc tttcatagaa tgcatcaaag tcaaaattca gatcatcttg cgagtatgta
     5041 actacagacg caatatagaa tctcaaatag tcggggttga atgcggaagt gaaatccttc
     5101 agaccaatat accagtttct actctttgat atcttttggt tttgcagcat aaggtggccc
     5161 ctagtgggga tatagtctgg aagcttgtat tctgaattga tgccaagacg tattgccggc
     5221 aagaagaggt agtggtggta gataatatcc ttgccaataa aatggtaaat ttccgattcg
     5281 ttccagaatt tcttgccgtt gcctccccta tccttgacga acttggcaag cgacgagatg
     5341 tagcataggt ggttatcaaa ccagccgtaa aacaccttac cctttgcctc gggcagaggt
     5401 attggcacgc cccatgagag gtcacgggtg atgtcccaat cctgcaaccc ttcgtttatc
     5461 cagttgatga catagttctt gacgtctggc tgcaggtgct ggttcgataa gagccactct
     5521 ttcagtttca cttcaaggct tgatagacgg aaaaagtagt gtttgctctc ctttttgaca
     5581 ggtggcctgc cgcatattgc acacttagga tccagtatct gatcagggac gcgcccacac
     5641 ttttcacaca aatctgaata ctggttttct gcattacagt aaggacatct gccaagtaca
     5701 tatctatcgg gtaaaaattt attgtcatag gtgcagtaga actgaattac attctggtcg
     5761 taaatgtggc cgttttcgca caattgtttg aaaacgtact ggacaaactc aacgttttct
     5821 ttggagcttg tcttgaaaaa aaaatcaaac gaaataccta atgatccaaa atcttctttg
     5881 tcctgtctgt tccagtattc tacataatct ttgggactct ttttttcctt ctccgctctt
     5941 atcaagatcg gagttccaaa atcatcagta gcacatatat ggtaagcctt caaccctttg
     6001 agtctcaaaa agcgtgtgaa aatatctgcc ggcagataag tggaggctat gtgacctagg
     6061 tgtatctcac catttgcata agggagtgcg cttgtaatga ttgcgttttc gctactgcta
     6121 ggatacggca ttacacttgc aatcaatgca gagcatttta acctaactgg aatgctggta
     6181 gcgctcttgc tcctcaatca tttgatctat cttgatgccc attgagtcaa gtgcatagct
     6241 tccaatatcc tgatctattt gttctggcac gctatgcact tttacttcca tcctgtcatg
     6301 attttttgca atgtataaaa tagataacag ctggttggca aatgacagag ccatcacttc
     6361 cggcgggtgg ccttctgcag caactaagtt gaccactctc ccctttgata ggaggaacaa
     6421 cctctttcca ttaacctcaa aacgctcgat atttggacta atctctactg gttggcagca
     6481 gctgatgtca taaaggctct tggtatcgat ctcgacatca aagtggcccg cgtttgccag
     6541 aatgacagca tccttcatct taagaaaatg ctccttcctt atcacgcacg tttgtccggt
     6601 gcatgtaatg aaaacgtcgc cagcaggagc agcgtctgac attcgcatta cctcaaatcc
     6661 atccatcttg gcttcgattg cttttatagg atccacttcg gtaacagtta ctatagcgcc
     6721 catgcccttt gcccgagttg ccacgccctt gcctacccag ccgtatccgc at
//
LOCUS       DASQTF010000193         5716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_444383,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000193 DASQTF010000000
VERSION     DASQTF010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444383"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VE308_04615"
     CDS             <1..305
                     /locus_tag="VE308_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00286.1"
                     /translation="NMRRTIVSAKYRAGLVPLLTQEETIESVEHSVLRMSTRRLMEAK
                     LGKTIYSITFYEKVKRAVIPIDKNGDFILLVSFDNEADYDSLIRNKIMPLVSYHQI"
     gene            complement(651..1463)
                     /locus_tag="VE308_04620"
     CDS             complement(651..1463)
                     /locus_tag="VE308_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZB00287.1"
                     /translation="MLATIIIAPNIANASLGLFSLFSENIVATYISTIPPGAAFEGNL
                     FHYYPEDIAVPSGTTIAWFNDDPGQLHTVTSGTPGDEEADSGRVFNSGGIPYGSFFQY
                     TFDEQGTFDYYCAFHPWITGTVTVSAAFIQGHHFEARIGTGPTLNLSENNNTLLDFKP
                     ITIAPQETIPITYNLSLLAPDGQRVFSESFFVLNNDLQVELINNATMDRTSVYGPDFS
                     DPITGTYHVMGDLFETSGIYSVRVEIVAIGNDQPDERIVDEFRMQVTTPQSL"
     gene            2021..3751
                     /gene="poxB"
                     /locus_tag="VE308_04625"
     CDS             2021..3751
                     /gene="poxB"
                     /locus_tag="VE308_04625"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF006591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HZB00288.1"
                     /translation="MPKKNVADLVIESLIAAGVKRVYGISGDSLNGITDSIREHDKDI
                     SWVHVRHEESAAFAAGSEAHLTGNLAVCAGSCGPGNTHLVNGLYDCYRSRVPVLAIAA
                     HIPIREIGSTYFQETHPELLFSECSQYCELISHTDQVPRVLEIAMRTAVSLRGVGIVV
                     LPGDVALQEAVSSRPFIPFEQPKATVCPSKEEITRLAEVLNSAQKVTILGGAGCAAAH
                     SELIQVAGLLQAPIVHAMRGKEYIEYNNPYDVGMTGLLGFSSGYHAMMDSDLLLMLGT
                     DFPYQQFYPPRAHIIQVDIRGEQIGRRTKVDLGLVGDVRETLVAVKPLLNQKSDKGHL
                     RASLNHYKKSRKDLDSYAVGEPGRTPIHPQYVAKIINDIAAHDAIFTCDVGTPTLWAA
                     RYLKMNGNRRLLGSFSHGSMANALPQAIGAQEVFPKRQVICLSGDGGFSMLMGDFLSL
                     RQLKLPVKIIVFNNGSLSFVELEMKAAGILTHGTSLIDTDFSKLANAAGILGLRAERP
                     EQVKPMLNQAIEHNGPALVDVVVNRQELLIPPSINLEQISGFTLYMIKAVLNGRGDEI
                     VDLAKTNLFR"
     gene            complement(4310..5209)
                     /locus_tag="VE308_04630"
     CDS             complement(4310..5209)
                     /locus_tag="VE308_04630"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZB00289.1"
                     /translation="MSKTIIERITPHLWFDNNAEEAAKFYTSVFKDSKIKNVTTLHNT
                     PSGTVEIFTIELLGQEFTLISAGPLFKFNPSVSFLVACSTKEEVDALWKKLSEGGTAL
                     MKLDEYPFSEMYGWVQDKYGLSWQVMFMGNHKIKQRIIPTLMFVGKQCGKAEEAINFY
                     ASVFGSARVGDIFRYSKGEDPDKEGTVKHASFMLEAQEFAAMDSARGHNFAFNEAISF
                     MVHCKTQKEIDYHWEKLSADPKAEQCGWLKDKYGLSWQIVPNLMDEMLKDSDKEKIAR
                     VTEAFLQMKKLDIAELKKAYGSG"
     gene            5417..>5716
                     /locus_tag="VE308_04635"
     CDS             5417..>5716
                     /locus_tag="VE308_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00290.1"
                     /translation="MTTKGCRQKQEIFIVSALDLLTANYTKGTEPDISDHKIVVELEN
                     ESSHPAILDLRFPFELKPDQVKAVEAWLVNQCRGSIIYGSGTGKTEIAFECARNAA"
BASE COUNT         1587 a         1262 c         1300 g         1567 t
ORIGIN      
        1 ccaatatgcg gagaaccata gtatcagcaa aataccgggc gggattggtt ccattgttaa
       61 cacaagaaga aacgattgaa tctgttgagc attcggtctt aaggatgagc accaggaggc
      121 taatggaagc aaagcttgga aagaccatat actctataac cttctatgag aaagtaaaac
      181 gggcagttat accaatagat aagaatggtg attttattct cctggtctca tttgataatg
      241 aagctgacta tgattcgcta attcgcaata aaatcatgcc ccttgtctca taccatcaaa
      301 tatagcaaaa tatggatatt ataggccatt gtcctaggag gacttgcagt cttattggct
      361 gtatatatct catcaagatt tcttgatcaa aataaatgaa atcaatccgt tgctttgatt
      421 ggcaggagac agttattctg gaacctgttg gccaagcaga gctcaagatt ggattcaaat
      481 gaatttttat ccaaattccg accagctcat tgccaactat aatgcgtcta aactatgaaa
      541 gttttttctc ttgaagctgt tgattcacag tcccaccaac tgagtccctc gaacaactac
      601 aaatgtagcc aatctgccaa ggagagggtg aaaggtaact tatcggtgga tcatagagac
      661 tgcggtgttg tcacctgcat cctaaattca tctactatcc tctcgtctgg ctggtcattg
      721 cctatcgcta cgatttccac tcgaactgaa tatatgccac ttgtttcaaa caagtcgccc
      781 ataacatggt atgttccagt aattggatca gaaaagtcag gtccatatac tgatgttcta
      841 tccatagttg cgttgtttat cagctcaact tgaaggtcgt tattgaggac aaaaaatgac
      901 tctgaaaata ctcgctgtcc atctggagct agcagcgaaa gattgtaggt tattgggatc
      961 gtttcttggg gtgcaattgt aattggtttg aagtccagca gcgtgttatt attttcagac
     1021 agatttagtg taggacctgt gccaattctg gcctcaaaat gatgcccttg tataaaggca
     1081 gcactgacag tcacggttcc agttatccaa ggatggaatg cacaataata gtcaaaagtc
     1141 ccttgttcgt caaatgtata ctgaaagaat gagccgtacg gtattccgcc agaattgaaa
     1201 actcttccac tatctgcttc ttcatcacca ggggtgccac ttgtcactgt gtgaagttga
     1261 ccaggatcgt cattgaacca tgctattgtt gttccagaag ggacagctat gtcctcagga
     1321 taataatgaa ataggttacc ttcaaatgct gcaccaggag ggattgtgga gatataagtt
     1381 gcaactatat tctcggaaaa caaggaaaac aagccaagcg atgcatttgc aatatttggg
     1441 gcaataataa ttgtagctaa cataaatgat gcaacaagaa ctgatgcaac tattgaaact
     1501 ttagagagac accgccgtct tcgttctctc gaattcatga gcaatagagg attatgtaga
     1561 taatatgcat tttagaagaa tcaataaggt tcggctcgtt ctttcaccaa ggacagatat
     1621 acatgtgtgc gtatcaaccg ccactccatt catcgattaa tctatgaaga ctatgttggc
     1681 aagtttactg agcgagcagc ctatattgtt tggggcggcc cccctgtgat ccaagcagtg
     1741 ttatcgggcc tttcttaaag aataaaaact gttttgtgac ggaacattgc ttttcaaccg
     1801 aaaataaaga agatgtgagt gagatacggg cgtatacaca attaggttat tttattatgg
     1861 ctcctcgaat gcagacatcc agacagaagg taagaccgta cccactcaac gatgatgaaa
     1921 gggccggagg gtcaacatcg aaaaatcgta taagagtctt accaaaagta tttttaatgt
     1981 attaagacaa ctccaaccac ctaagagtgt caaataagtc gtgccaaaga agaacgttgc
     2041 cgatctcgtg attgagtcgt taatagcagc cggagtaaag agagtatatg gtatctccgg
     2101 cgattctctg aacggaatta ctgattctat aagggaacat gacaaggata tttcctgggt
     2161 gcacgtccgc cacgaggaga gcgccgcgtt tgcggctgga tccgaagctc atcttacagg
     2221 aaatcttgct gtctgtgcag gaagctgcgg cccaggaaac acacatctcg ttaatggact
     2281 ctacgattgc tatcgcagca gggtgccagt cctagcaatt gcagcacaca tccccataag
     2341 ggaaatcggg agcacatact ttcaggagac tcatcctgaa ctactattca gcgagtgcag
     2401 tcaatactgt gagctgattt cccacactga tcaggttcca cgtgtccttg agattgcaat
     2461 gagaactgct gtttctctcc gtggagttgg gatcgttgtt ctccccggag acgttgcatt
     2521 gcaagaagct gtttccagca gaccattcat acctttcgag cagcctaagg caacggtctg
     2581 cccgtctaaa gaagaaatca caaggcttgc tgaggttctc aattccgcgc aaaaagtaac
     2641 cattcttggt ggcgcaggtt gtgctgctgc tcattcagaa ctgattcagg ttgcaggtct
     2701 tctccaggct cctattgtgc atgcaatgcg aggaaaggag tacattgaat acaataaccc
     2761 atacgatgta ggtatgactg gactacttgg cttttcatct ggatatcatg cgatgatgga
     2821 ctctgacttg ctactaatgc ttggcaccga ctttccatac cagcagttct atccgcctcg
     2881 tgcccacata attcaagtcg acattcgagg cgagcaaata ggtagacgaa caaaggtcga
     2941 tctcggactg gtaggtgatg tccgtgaaac tttggtcgca gtgaaacctt tgctcaatca
     3001 gaaaagtgac aaaggacacc tgagggcatc attgaatcat tacaagaaat ctcgcaagga
     3061 tttggactct tatgcagtgg gtgagcctgg acgaactcca attcatcctc agtacgttgc
     3121 aaaaatcatt aatgatatcg cagcgcacga tgcgatcttc acatgcgatg ttggtacccc
     3181 gactctttgg gcagcacggt accttaagat gaacggcaac cggaggttac tgggatcctt
     3241 ttcacacggc tcgatggcaa atgccctacc acaggcaatc ggagcacaag aagtttttcc
     3301 caaacgtcag gtaatctgtc tgtcaggcga tggggggttt tcgatgctca tgggcgattt
     3361 tctttcatta agacagctga agctgcctgt aaagattatt gtattcaaca acggatccct
     3421 gagttttgtc gaactagaga tgaaggctgc agggatactg acccacggca caagtctcat
     3481 tgacactgat ttctctaagc tggctaatgc tgctggaatc ctgggcctcc gcgcagaaag
     3541 acctgagcag gtgaagccaa tgctgaacca ggccatagag cacaacggcc cagcattagt
     3601 agacgttgta gtgaaccgcc aggagttgct tatacctcca agcattaacc tcgagcagat
     3661 aagtggcttt actctttaca tgatcaaggc ggtgctgaac ggccggggag atgagatcgt
     3721 ggatctggct aagacaaacc tgttcaggta ataacatgga aagattagtc gactacggag
     3781 gtagtagaat cgtattcgta tattgaattg gctgattcac tatgttatgc tttgttatgt
     3841 catgaattgc acttcggacc gtatgattca cactttacta tatcaagcac gaacttttga
     3901 gacgtgccag acgagccagt acgggggact gaaacggcgt aacctacaac aaaaagcgtg
     3961 tgtgaatgtg ggaatctgtg tgtatgtgca tgtatgtgtg tgagcttctg gccgacagcc
     4021 agcagagaag cggctgagat ctaatttcat tggatcctca atactcgaat aaaaggcaga
     4081 aaattttgtc tttggattca aatgaatttt tatccaaata ccgagggtta cacttctttg
     4141 caggattgtg tataaattct gaaggtcttt gtcttgtccc tcagcggcct tgttttgtat
     4201 caataactgt ccatatgatc tagtttatac tagttctcca agaagcttcg ataatcagca
     4261 ccggcagcac aaatccatca atataccctg ccatactacc acctctcgat tatcctgatc
     4321 cataagcctt cttcaattcc gcaatatcca gctttttcat ttggagaaac gcttctgtca
     4381 ctcgagcaat tttctccttg tcactgtcct tgagcatctc atccatgaga ttgggaacta
     4441 tctgccacga gaggccgtac ttgtctttga gccaaccgca ctgttctgcc tttggatctg
     4501 ctgagagttt ttcccagtgg tagtctattt ctttctgggt cttgcagtgc accatgaatg
     4561 aaatagcttc gttgaatgca aagttgtgtc cacgcgcact gtccatggcg gcaaactcct
     4621 gggcttcgag catgaaggag gcgtgcttta cggttccttc cttgtcgggg tcttcgcctt
     4681 tgctgtagcg gaagatatcg ccaactcttg cactaccgaa taccgatgcg taaaaattga
     4741 tcgcctcttc cgcctttccg cactgcttgc cgacaaacat gagcgtcgga atgattctct
     4801 gttttatctt gtggttgccc atgaacatca cctgccatga aagaccgtac ttatcctgca
     4861 cccagccgta catctcgctg aacggatatt cgtcaagctt cattagcgct gtgcctcctt
     4921 cagacagttt cttccacaac gcgtcgacct cttccttcgt gctgcaggcg acaagaaatg
     4981 agacggatgg attgaatttg aagagcgggc ctgcgctgat gagcgtgaat tcctgaccca
     5041 gaagttcaat ggtgaaaatc tcaaccgttc ccgagggggt attgtgaagc gttgtcacgt
     5101 tcttgatttt tgagtctttg aaaacagagg tgtaaaattt agccgcttct tcggcattgt
     5161 tatcgaacca cagatgcggg gtgattcgtt cgataatagt tttgctcata tctctgccct
     5221 atagcaaatc acataaaagg cattactccc cttggggaag tctttgagcc atcgactgac
     5281 tagctacagc agaaccatag atgacttcat tgaacatcaa cgggcctgaa agaagtaaac
     5341 catatcactt atggacatcc cctcccctct aggtccttct ctgttaatat atcaataagg
     5401 ttcaaggctt gcttctatga ccacaaaagg atgcaggcaa aagcaagaga tcttcattgt
     5461 ctctgctcta gatttgctta ccgcgaatta tacaaaaggc acggaaccgg acatttcaga
     5521 tcataaaatt gtagtggagc tagaaaatga aagcagccat cctgcaattt tggacctcag
     5581 gttccccttt gaattaaagc ctgaccaggt aaaggcagtg gaggcttggc ttgtaaatca
     5641 atgcagaggg tcaattattt acggcagtgg cactggcaag acagagattg catttgaatg
     5701 tgccagaaat gcagct
//
LOCUS       DASQTF010000194         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1091956,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000194 DASQTF010000000
VERSION     DASQTF010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091956"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VE308_04640"
     CDS             complement(<1..844)
                     /locus_tag="VE308_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZB00291.1"
                     /translation="MTTTTTTKDLNVAVIVKLEPDFSEGNVSYNADGTLNRAETKSIL
                     GPHSAIASNAAFYAKVKYGARVAVGTMGPPIAESALQQAQLLSDADELHLYSDRIFAG
                     ADTLATAEVLKAGISKVEGDGKVDIVFSGHRASDGETGQTGPQTAWKLGYTFLGNVID
                     YNVDLQSRFVRAKRLISLQGFYDIIEEVEAPLPVFISIDPSYKALFNTVSQRLAFEKY
                     QKEAKSRAENYKQYLKVLNTQQLGIDPKLVGLPGSPTIVYKVERIPKAKASRKAEVID
                     GANHD"
     gene            complement(906..1604)
                     /locus_tag="VE308_04645"
     CDS             complement(906..1604)
                     /locus_tag="VE308_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00292.1"
                     /translation="MGSSLDIIRVGKREFLDFLQNEGKVRLDDYIEGAVEVAEEVHSD
                     LMREDGTSPFLETHTWPVTMDVVRHYRSVNRNITSVEIATAILHDVMEDDERILNLYE
                     SKSYGFEANLAYRFGSRVGDIASELKVKPLENYPGANDIERQLERFLEYCDILRDADY
                     DVKVIKLADRVNNMSFIRHVPGHDKIRRYMREAEDFYIAYTMLPPKMPNFYQSMRAAY
                     EELRALSTQKLIAA"
BASE COUNT          628 a          559 c          546 g          722 t
ORIGIN      
        1 gatcatggtt tgcaccatcg ataacttcgg cttttctact agcctttgcc tttggtatgc
       61 gctctacttt gtagacaatc gttggggagc cgggcaaccc aactagtttt ggatcgatac
      121 ccagctgctg cgtgtttaga accttcaggt attgcttgta gttttctgcc cgggattttg
      181 cttctttctg gtacttctca aacgctagcc tctgtgacac agtgttaaat aatgccttgt
      241 atgatggatc gatggatatg aaaaccggaa ggggtgcctc aacctcttca ataatatcat
      301 aaaatccttg aagtgagatc agacgctttg ccctcacaaa tctgctctgc agatcaacat
      361 tataatctat gacattgcca aggaaggtgt agcccagctt ccaagcagtc tgaggccctg
      421 tctggccggt ctcgccgtca gatgccctgt gaccggaaaa cacgatatcg actttaccat
      481 caccttccac cttagatatg ccggccttca gcacttcggc ggtagcaagc gtgtcggcac
      541 ctgcaaagat cctatcgcta tacaaatgaa gttcgtctgc atcggagagc agctgcgctt
      601 gctgtagcgc gctctctgca atagggggac ccatcgttcc aactgcgacc cgggcgccat
      661 atttgacctt agcataaaat gccgcatttg atgctatagc actatgaggt ccaaggatac
      721 tctttgtttc tgctcgattg agcgtgccgt cggcgttgta gcttacattt ccttcagaga
      781 aatcaggctc taatttcaca ataactgcaa cattgaggtc ttttgttgtt gttgttgtcg
      841 tcaatactta tcacatcgtt tatccccatc aagtactatc attaaattgt ttgtaaagaa
      901 agaagttatg cggcgatcag cttttgagtc gataatgccc tcagctcttc ataggcggct
      961 ctcatgcttt gatagaagtt aggcatcttt ggcggcagca tggtgtatgc gatgtaaaag
     1021 tcctctgctt cccgcatgta gcgccttatt ttgtcatgtc ctggaacgtg cctgataaat
     1081 gacatgttgt tgactctgtc tgccagtttg attaccttga catcgtaatc ggcatcacga
     1141 aggatgtcgc agtattctaa aaagcgctcc agctggcgct ctatatcgtt tgctcctgga
     1201 tagttttcaa ggggcttgac cttgagttcg cttgcaatgt cgccgaccct gctgccaaat
     1261 cggtatgcaa gattcgcttc aaatccatat gacttggatt cgtatagatt caagattctc
     1321 tcatcgtctt ccatgacgtc atgcagaatt gcggttgcaa tttctacgct tgtgatattg
     1381 cgattgactg agcgataatg ccttacgaca tccattgtga ccggccatgt gtgggtttca
     1441 aggaatggcg aagtgccatc ttcgcgcatt aggtcagaat ggacttcttc tgcgacctca
     1501 actgcgcctt caatgtaatc gtccagtcgc actttaccct cattttgcag aaaatccaag
     1561 aactcgcgtt tgcctactcg aataatgtcg agactgctgc ccatacctct acatagcacg
     1621 gtagttggac aaaagttctt tggtagatga atagaataaa aaatttttat aagattagtt
     1681 ttcccaaatc tcggatctat atcaaataga gaagaagcct tgaaggctca tttgtagttc
     1741 ttatatatgg acctgttgaa gctcccttct aaagatgcct tagtaaggtc cctttagatg
     1801 ttaatgttct ctaaagaagc catggtccca tgcctgctag atatgtgtta gaaatgaaga
     1861 gagtgttagc catagtgtat ctcccatttc aaagctcaat gtccctgcct cgaattttaa
     1921 gctgtgatat cacagcttga agcctgtggg caattggcaa tcatcatata caagaataga
     1981 gataagcatt cgaaacttgg gcagatattc aagagttgtc atgtattagt atcatttttt
     2041 gcactaagat attcgaattg gtctttgtcc cgtagagtag gttgccagta tatctttaag
     2101 ctattcccaa aacaagcgct tttcaaagat cggacttctc actacaaagg cgaacattat
     2161 cttgtcctcc tgcaagatgg aacttgtcag aaaaatagct ggcggacaaa tattaccaaa
     2221 cgcacaaaca gagccgaagc acaaggataa tgatgtgagg gattagccat ataagcccgt
     2281 ttggaaaaat aggtgttcta gcactttgct tacaaactct acaggcttta atctggcaaa
     2341 agatgccttt cctcccagcg gagaccagcc tgaagcgata gagcagcttg tcagaggtgt
     2401 gagtaagggt ggacgccaga cactcctcgg cgtgactggc agtggtaaga cgttt
//
LOCUS       DASQTF010000195         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_71301,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000195 DASQTF010000000
VERSION     DASQTF010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71301"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VE308_04650"
     CDS             complement(<1..538)
                     /locus_tag="VE308_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HZB00293.1"
                     /translation="MAVEKKFYEMSREERLDYIRKATGISGDIEAMLRPLSFEDANRM
                     VENAIGIMSIPMGIATNFVINGRECVIPMAIEEPSVIAAVSKAAKIAQVKGGFIAEAD
                     DSLMIGQVQVVRLGNVKHAVTKISRNKKQLLAIANSNSRSVTAVDLQVRQLRDKSPNN
                     MGNILLVELIIDTKDAMGA"
     gene            complement(549..1667)
                     /locus_tag="VE308_04655"
     CDS             complement(549..1667)
                     /locus_tag="VE308_04655"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZB00294.1"
                     /translation="MDKERRLAIVAIAAVTVAAVATAFFTPSQTTLPIPEPGQSDGNN
                     NTRSGVQMLAENLDVPWAIDIAEDGRLFFTERAGRIMAIDKNGSLLSDPVAYINVMQN
                     SESGLLGLVLHPNFTDNHLLYVYHTYSNDSAVLNKVLMLTENDNKIVESKVIIDNIPA
                     ADRNDGGRIKFGPDGKLYIATGDARQPDLAQDARSLAGKILRLNSDGSIPKDNPFEGS
                     PVYSYGHRNVQGLAWHPLTGDLYASEHGFEGNDEINLINPGTNYGWPIEDCNAEKFEK
                     PIVCFNPAIAPAGMTFAASDSLGYQNGILLATLKAQHIRLIDLESDIESNILTGFGRI
                     RDLVEAPDGSLYVVTSNKDGRAVPAQNDDRILRIISPQ"
     gene            1757..2854
                     /locus_tag="VE308_04660"
     CDS             1757..2854
                     /locus_tag="VE308_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZB00295.1"
                     /translation="MKVSEIMVSDVVTLTSEDTIAKALNLMANRSINQIPTVDENGKY
                     LGMIFAKQIINSTAQPSSKLKSYIVSTSTLSRDQDIEKAAQLIIGSGNRAMPVVENGK
                     LGGIVSETDLVSTADFGHAIVDEVMSGAIVIEENNTVADAISKMRRYNISRLPVINTK
                     GILRGTFSILDIAKIIAIPRERTSKSAAISGGTAGIKDVKVKDITRRAISVERGTKLN
                     VMTDHFRQNEEVVVVGDGRPMGIVTPKDALELILPKKSSEPIIHMAHFEDGQDRIEIQ
                     EQLARFLEKIQGKLGDVRSIVVYADKHKTRKYSLRTRLITSDGVIDAKAVGYDPLSAS
                     KELIARLERRIKSEHSQRVKDRQHRESPRKM"
     gene            complement(2868..2992)
                     /locus_tag="VE308_04665"
     tRNA            complement(join(2868..2918,2935..2992))
                     /locus_tag="VE308_04665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2936..2938),aa:Ser,seq:tga)
     gene            3516..3794
                     /locus_tag="VE308_04670"
     CDS             3516..3794
                     /locus_tag="VE308_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00296.1"
                     /translation="MQERKGVQKLKSISVLVPFERVQASLSEIGFLLEDSVTIVADGK
                     FWTKMLYIRDNETISVSEEIGDAYPKDPIITICGSKVTISKVVKGIGG"
BASE COUNT         1125 a          851 c          904 g         1043 t
ORIGIN      
        1 ttgcacccat ggcgtccttg gtgtcaatga tcagctctac cagcaggata ttgcccatat
       61 tatttggact tttgtcgcgc agctgtcgca cttgtaagtc gaccgcagtc acagacctgc
      121 tattagaatt ggcgatggca agtagctgct ttttgtttct tgatatctta gtcactgcat
      181 gcttgacgtt gccaagcctg acaacttgca cctgacctat catgagagaa tcatcagcct
      241 cagcgataaa cccacccttg acctgggcta tttttgcagc cttgctaact gcagcaataa
      301 cggatggctc ttctatagcc attgggatca cacattctct gccgtttatg acaaaattcg
      361 tggcgatacc cattgggata gacataatgc caatagcatt ttctaccatc ctatttgcat
      421 cttcaaatga aagtggtctg agcattgctt caatgtcgcc agatattcca gtagcctttc
      481 ttatgtagtc aaggcgttct tccctgctca tttcatagaa ctttttctcc accgccatct
      541 ctgtcatgtt actgcgggct gatgatcctt aaaatcctgt catcattttg cgcgggtact
      601 gctcttccgt ctttattact tgtgacgaca taaagtgacc catcaggagc ttcaacaaga
      661 tctcgtattc ttccaaagcc ggtcagaatg ttgctttcaa tatcagattc caaatcgata
      721 agacgaatgt gttgagcctt cagagtggca agcaatatgc cattctggta gcctaagcta
      781 tctgaagcag caaatgtcat tcctgcagga gctattgcag gattgaagca tacgatcggc
      841 ttttcaaact tctcggcatt gcaatcctcg atgggccagc cgtagttagt gcccggatta
      901 atgagattga tctcatcatt gccctcaaac ccatgctcgc ttgcgtatag atctcctgtc
      961 agagggtgcc atgccaagcc ctgaacgttg cggtgtccgt aagaatacac aggcgagcct
     1021 tcaaaaggat tgtctttggg gatgctgcca tcagagttga gtcgcaggat cttgcctgca
     1081 agtgatctgg catcttgggc aagatcgggc tgtcgtgcat cgcctgtggc gatatacaac
     1141 ttgccatcag gtccaaactt gatcctacct ccgtcatttc tgtcggcagc aggaatgttg
     1201 tcgattatga cttttgattc gactatcttg ttgtcatttt ctgtaagcat taggactttg
     1261 ttcaaaacag cagaatcatt ggagtaggta tggtagacat aaagcagatg gttatcagta
     1321 aagttgggat ggagcaccaa tcctaatagg cctgattcgc tattctgcat gacattgatg
     1381 taagctacag gatctgaaag aagactgcca ttcttgtcta tagccattat cctgccggcc
     1441 ctttctgtga aaaagagcct gccatcctcg gctatgtcaa tggcccaagg cacgtcaagg
     1501 ttctccgcaa gcatctgaac gccagagcgg gtattattgt tgccgtcact ctgtccaggc
     1561 tcaggtatgg gaagtgtagt ctgtgagggc gtgaaaaaag cagtcgcgac tgcagccacc
     1621 gttacagcgg ctattgccac tattgccagc cgccgctctt tatccattgt cctagttaag
     1681 gacggagaag cattataatt atgctgcctg cgaatgtgta aggtttaaaa taacaagcac
     1741 cgagatgttc tattgaatga aagtatcaga aataatggtg tcagacgtcg ttactctcac
     1801 ctctgaggac acgattgcaa aagcattaaa ccttatggcg aacagatcaa ttaatcagat
     1861 acccacagta gatgaaaacg gcaaatatct cggtatgata tttgctaagc agataatcaa
     1921 ctctactgca caaccttctt caaaattaaa aagctacatc gtcagcacgt cgacattgag
     1981 tagagatcag gacattgaaa aggctgccca gcttataatt gggagtggaa accgcgctat
     2041 gccagtggta gaaaacggca agcttggagg tattgtcagc gaaactgacc ttgtatcgac
     2101 ggcagacttt ggacatgcta ttgtagacga ggtcatgtct ggcgcgatag taatagaaga
     2161 aaataacacg gttgctgatg caatctctaa aatgagaagg tacaacattt cacggcttcc
     2221 agtgataaat acaaaaggaa tcctccgcgg cacatttagt atacttgaca tagctaagat
     2281 aattgcgatc ccacgtgagc gcacaagcaa gtctgcagca atctctggtg gaactgcggg
     2341 catcaaagat gtcaaagtaa aagatatcac aagaagagcc atttcagtcg agcgtggcac
     2401 caagctcaat gtcatgacag accactttag acagaatgaa gaggtggttg tggtggggga
     2461 tggaaggcca atgggcatag tcaccccaaa ggatgcactt gagcttattt tgcccaaaaa
     2521 gagcagcgag cctatcatcc acatggcaca ctttgaagat gggcaggaca gaatagaaat
     2581 tcaagagcag cttgcacgct ttttggaaaa gattcaaggc aagcttggag atgtgagatc
     2641 tatagtcgtg tatgcagaca agcacaagac gcgcaaatat tcactcagga cgcggcttat
     2701 cacgtcagat ggtgtgatag atgcaaaggc ggtgggctat gatccactca gtgcgtcaaa
     2761 ggagctcata gcacggcttg agcgcagaat caagtccgag catagccaga gagtaaagga
     2821 ccgacagcac cgtgaatctc caagaaaaat gtgataacaa taacaaatgg cgcggatgag
     2881 gagattcgaa ctcctgatcg cccgagggcg aatcggctca ccggcgcttg tcatctcaag
     2941 gccgacgcat tactatgttc gcctagaaag aagaccactc tgccacatcc gcgctaccag
     3001 tttcttttaa gttcctacta ttatatgtta attatgtgat agaattgctt ttttatagat
     3061 gtataatcct tcaagaatat tggataattt gttgttgttg tatgtataaa cggatatttc
     3121 gaaaatgagc ctgaatttgg attctgaatt tctttttaat acttcttaat attgcaatgc
     3181 ataaaattag acggtatagc gcatcatcat tatactgtca tacagtacac gacctaaaag
     3241 gtgtgcaata gacggaagcg ataggccagc agctgactat gatataacag atcgtcacat
     3301 caacaggaca gatgcaaagg atgaatcctt gtttgcagct atagtgtgta ctttgcaaat
     3361 catgatgatc ctaaagaaag atgttcacaa acgcagtaat ggctatagct ctttcttctt
     3421 caagggggaa aagtatgaca ggaatattta caacagaaac ctatttcacc taacctgcga
     3481 ccaattttta tacctgaaac acaaagaaaa tatctgtgca agaaaggaaa ggcgtacaaa
     3541 aactgaagtc aatttctgtg cttgtgccgt ttgagagggt gcaggctagc ctttctgaaa
     3601 ttgggttttt gctagaggat tctgttacaa ttgttgctga cggtaagttc tggaccaaga
     3661 tgctgtacat cagggacaac gagacgatca gcgtctcaga agagattgga gatgcctacc
     3721 caaaagatcc gataattacc atatgtggct ccaaggtgac aatatcaaag gtagtcaagg
     3781 gcattggcgg ataaaattgc ctctttacga atgtaacgag caccagtttg tggagaacat
     3841 ccggcgtctt ttagaatcaa aggaaaagtt ccttgtgaat cgcaaaatga tgctgcatga
     3901 tgacgctaag tacggcccag cca
//
LOCUS       DASQTF010000196         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_992641,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000196 DASQTF010000000
VERSION     DASQTF010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992641"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VE308_04675"
     CDS             complement(<1..821)
                     /locus_tag="VE308_04675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZB00297.1"
                     /translation="MNAFYPSCEELRDPSLKGKAHAVIMTDEKTGEIMKGAVSSCSYE
                     ARRYGVSSAMSLSKAKSLCPDLILHPVDIPYYRGISEQVMNAIQEYADTLEQASIDEA
                     FLDCTTRIQSESPETYAGKIKKTIKDKCGGLSCSIGLTSTKSAAKIASDFEKPDGLTV
                     VYPTKLKKFLAPLDVGTVAGIGPKTQMALKELGIDTLGQLAKADVQKLKERFGKNGYW
                     MWKVANGTDDEIVAPRTDNVSLSTENTLAIYTRDKEKIHGHLDELVDDIYQRVRE"
     gene            complement(1132..>2076)
                     /locus_tag="VE308_04680"
     CDS             complement(1132..>2076)
                     /locus_tag="VE308_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZB00298.1"
                     /translation="LQDADFSFDTVRGIRLKLVGIFEMAEKCRSMAQKATEQQQQQQQ
                     QQQVLLMPEDKWLVSRLQRTIAETSASMDRLRIREAIYYTLYSLDRDLQWYLKRATAR
                     KRENITGMLAEFLNLQVRMLAPFAPFTAEEVWELMGNSQSITTARWPVVEEEKIDISA
                     EESEFLISGLLSDIQNIVKVTKIAPTKIVIYASAAWRIQVYNAILANILEGRINFGQI
                     MKHLIANPDTAKAKSDPKMVQKMMEDILSAPLEARNRRLKLTGFNEVATIQDAQLLLS
                     DEINSAKIVVYSEEDPSKYDPKSRAKSARPFKPAIYIE"
BASE COUNT          511 a          458 c          394 g          713 t
ORIGIN      
        1 tgttctctaa ccctttggta gatatcatca actaactcgt caagatgccc atgaatcttt
       61 tctttatctc gagtatagat agccagcgta ttctcggtac taagagatac attgtcggtt
      121 cttggtgcta ctatctcgtc atcagtgcca ttagcgactt tccacatcca ataaccattt
      181 tttccaaacc gttcttttag tttctgaaca tccgctttag ctaactgccc cagtgtatca
      241 atgcctagct cttttaatgc catttgtgtt tttggtccaa tgccggctac tgtgcccaca
      301 tcaaggggtg caagaaattt ctttagctta gtaggataaa caacagttag accatcgggt
      361 ttctcaaaat cagatgcaat ttttgcagct gatttcgttg atgttaatcc tatagagcat
      421 gacagtccgc cacatttgtc tttaattgtc ttctttattt ttccagcata ggtttcggga
      481 ctttcgcttt ggattcttgt tgtacagtcc aagaacgctt catctatgct tgcttgttct
      541 agagtatctg cgtattcttg tattgcgttc attacctgct ctgatatccc tctgtagtac
      601 ggtatatcaa cagggtgtag tattaagtct gggcataatg atttagcttt tgaaagtgac
      661 atggcagaac taacgccgta tctccttgct tcatatgagc acgaagaaac tgctcctttc
      721 ataatttctc ctgttttttc atctgtcatg atgactgcat gagcctttcc cttcaatgaa
      781 gggtctctta gttcctcgca ggacgggtag aaggcattca agtcaataca tgctatgacc
      841 ctaccgatcc acttttcctt ggaattaaat atcttcgtgc ccagttatga tataagagta
      901 attcttgatt ttagcatttc tgtaatagac ttagattcat taatcttgca taaaatatgc
      961 attctcgtac tgagatagat tgaaaaagtc gttctccaat tgttttgcag gtttgaagac
     1021 tcgtttttta tcataaagct ataagcttta acaattacaa ttctactctg aaataaaact
     1081 cgtaataact acaacattgc aacgtcttag atttatagga tatagagctg attattcgat
     1141 atatatcgca ggcttaaaag gccttgcaga ctttgctctt gattttgggt catattttga
     1201 gggatcttcc tcggaataga cgactatctt tgcactgttg atctcatctg acaatagtaa
     1261 ttgcgcatcc tgtattgtgg ctacctcatt aaaccctgtc agcttgagcc gcctgttcct
     1321 tgcctcaaga ggagccgaca gaatatcttc catcattttt tgcaccatct taggatcact
     1381 tttggccttt gcagtatccg ggtttgcaat cagatgcttc attatttggc caaagttgat
     1441 cctgccttcc agtatgttgg caagtattgc attgtacact tgtattctcc aggcagctga
     1501 agcatagatt acaatcttag taggtgctat cttagtgacc ttgacaatgt tttggatatc
     1561 tgaaagcagg ccagagatga gaaattccga ttcttctgcc gagatgtcta tcttttcttc
     1621 ctctaccaca ggccaccttg ctgtagttat tgattgactg ttgcccatga gttcccagac
     1681 ttcttctgct gtaaatggcg caaatggtgc aagcattctg acttgaaggt taagaaactc
     1741 agcaagcatt ccagttatgt tctcacgttt cctagcagta gctcgtttta gataccattg
     1801 caaatctcgg tcaagtgaat acagagtata gtatatcgct tctcttatcc ttaatctgtc
     1861 catcgatgct gatgtttctg ctatcgtgcg ctggagccgg ctgacaagcc acttatcttc
     1921 cggcatcagc agcacctgtt gttgctgttg ttgttgctgt tgctgctccg ttgccttctg
     1981 cgccatgctc ctgcactttt cagccatttc aaaaatgcca acaagtttca acctaatgcc
     2041 tcgaacagta tcaaaggaaa aatcggcgtc ttgaag
//
LOCUS       DASQTF010000197         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_346133,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000197 DASQTF010000000
VERSION     DASQTF010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346133"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1502
                     /gene="aspS"
                     /locus_tag="VE308_04685"
     CDS             162..1502
                     /gene="aspS"
                     /locus_tag="VE308_04685"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HZB00299.1"
                     /translation="MDELLQEEDLRNWRRTHYSKEIALPIGGDGEVVIMGWVASIRDH
                     GNIQFIILRDMYGEIQITVKKRECSESLFQLAKEVKEHSSIGVRGKVRPQEKVPNGAE
                     IVPIEIRIFSIARKAAPFLVQSRTSTVGIDTRLDLRAVDLRRNYLGSIFRIRQTVLNS
                     VREFLSDQKFIEVNTPKMIATATEGGAALFPIFYYDREAFLAQSPQLYKEQLTMAFES
                     VFEIGPIFRAEPSRTNRHLSEAISIDVERAFVDYSDIMSLLERMILKIVDTLKEKNQD
                     DLEYLQLRLPSLDVPFSKYSYSYIIDRLQEAGERIQWGDDISPQIMKSIQHEHLNDFY
                     FIIDWPTSTKPFYIKPSAEDSRICESFDLMYGGLEVSSGSTRINHKDQLVERMAKQGL
                     KPEAFDYHLRVFDYGVPPHAGFGLGLERLIMALTKVENIRDVTLYPRDIDRLAP"
     gene            1510..1782
                     /gene="gatC"
                     /locus_tag="VE308_04690"
     CDS             1510..1782
                     /gene="gatC"
                     /locus_tag="VE308_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZB00300.1"
                     /translation="MVVTREEVKHLGWLAKLEMSDSELQRYTAQIEEIISYLNKLDTI
                     PLEQVKPIAMKKKFSELREDAPENFKDTVLGTGYRKDGFVKGPRMV"
     gene            1784..>2721
                     /locus_tag="VE308_04695"
     CDS             1784..>2721
                     /locus_tag="VE308_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZB00301.1"
                     /translation="MQLYTLEAGQVVQGIKSREFSAEEYISQLFERIEKIEPKVNAFV
                     TINKEEGFDRARRLDKKIRDDEQNGPLAGVALSIKDNICTKGMKTTCASKMLESYIPA
                     YDATVIKRLMAAGAIIIGKANLDEFAMGSTTEFSRHGPTRNPWDISRVSGGSSGGSAA
                     SVAALECTASLGSDTGGSVRCPASFCSVVGLKPTYGLVSRFGLVSYANSLEQIGPVGR
                     TVSDVVSILNVIAGSDENDHTTTNSNKPTYSLQERKGLRVGLVMEFTEGADTAVSKMI
                     YKAADTLEQQGCNCEEVSLPSVRYALASYYTIATAEA"
BASE COUNT          824 a          582 c          669 g          646 t
ORIGIN      
        1 attgtcaaaa aacaagccgt cagcagagtt ctcgacagca ttgcaagaga aatcttgtgc
       61 taaccccgca attccattgc tgctggccac caccactagc acacatccac acactgatgc
      121 tggtaaggtt aaattaacct cttcgatata cccctgttta attggacgaa ttgctgcagg
      181 aagaggatct tagaaattgg cgaagaaccc actattccaa ggagatagcc ctccctatag
      241 gaggggacgg cgaagtcgtt atcatgggct gggttgccag tataagagat cacggcaaca
      301 tccagtttat catattaagg gatatgtatg gtgaaatcca gatcacagta aagaagcgtg
      361 aatgcagtga atcgcttttc caactggcca aagaggtcaa ggagcacagc tccattggag
      421 tacgcggcaa ggtgaggccg caggaaaagg ttccaaacgg cgctgaaata gtgccaattg
      481 agatcaggat attttcaata gcaagaaagg cggcgccttt ccttgttcag agtaggacat
      541 caactgtagg cattgatacc cggcttgacc ttcgtgcagt tgatctgcgg cgcaattatc
      601 ttggatcgat atttcgtata cgtcagactg tgctaaattc tgtcagggaa tttctctcag
      661 atcaaaaatt cattgaagtg aacacaccaa agatgatagc cactgcaaca gaaggtgggg
      721 cagcactttt tccaatattc tattacgaca gagaagcctt ccttgcgcag agtcctcagc
      781 tatacaaaga acagttaaca atggcatttg aaagcgtatt tgagatagga cccatttttc
      841 gcgcagagcc ctcccgcacc aatagacatt tatcagaagc aatttcaatc gatgtcgagc
      901 gtgcatttgt tgactatagt gatatcatgt cgctgctcga gaggatgata ttaaagattg
      961 ttgatacact aaaagaaaag aatcaagatg atctggagta tctacagctc cggctaccgt
     1021 ccctagatgt gccgttttca aagtatagtt attcttacat cattgacaga ctgcaggagg
     1081 ctggcgaacg catacagtgg ggagacgaca tttcgccaca gattatgaag agtatacagc
     1141 atgagcacct caatgacttt tacttcataa ttgactggcc aacttcgacc aagccgtttt
     1201 atatcaagcc aagcgctgaa gacagcagga tatgtgagtc atttgacctg atgtatggag
     1261 gacttgaagt ttcatcaggc agcaccagaa taaaccacaa agatcagctg gtggagcgca
     1321 tggcaaagca gggtctaaag cctgaagcgt ttgactacca tctgcgggta tttgattatg
     1381 gggttccacc acacgctggg tttggtcttg gacttgagcg gcttataatg gcattaacaa
     1441 aagtcgagaa tatccgtgac gttacacttt atccaagaga catagatagg ctggcgccat
     1501 gataataata tggtagttac aagagaagaa gtaaagcatc ttggatggct tgcaaagctc
     1561 gagatgtcag actcagagct acagcgctat accgcacaga tagaagagat aatcagctac
     1621 ttgaacaaac tggacacgat accacttgag caggtcaaac caatagcaat gaaaaagaaa
     1681 ttttcagaac ttcgggaaga tgcacctgag aatttcaaag acactgtgct tggaacagga
     1741 tacaggaagg atggctttgt gaagggtcca aggatggtct gaattgcaac tctataccct
     1801 tgaggccggg caggtagtcc aaggcatcaa aagcagggaa ttttctgctg aggaatatat
     1861 ttctcagctc tttgaaagga ttgaaaaaat agaacccaag gtaaacgcct ttgtcacaat
     1921 caacaaggaa gaagggtttg atagagcgcg caggctggat aaaaagatca gagacgatga
     1981 gcaaaatggc ccgcttgcag gagtcgcatt gagcatcaag gacaatattt gcacaaaagg
     2041 gatgaaaact acatgtgcct caaagatgct tgaaagttat atccctgcat acgatgcaac
     2101 agtgatcaag cgactgatgg ctgctggtgc aatcataatc ggcaaggcaa acctcgatga
     2161 gtttgcaatg ggttctacta ctgagtttag ccgacatggc cccactagaa acccgtggga
     2221 tatcagcaga gtctcgggtg gctcgtcagg cggaagcgca gcaagcgtgg ctgcccttga
     2281 atgcactgca tcgcttggtt cggacaccgg aggctcagtc agatgtcctg ctagtttttg
     2341 ttccgtagtt gggctcaagc ctacatacgg ccttgtgagc agatttgggc ttgtatcgta
     2401 tgcaaacagc ctagagcaga taggaccagt tggcaggaca gtatcggacg tagtttccat
     2461 tctaaatgtg attgccgggt ctgatgaaaa cgatcacact acaaccaaca gcaacaaacc
     2521 tacatattca ctacaagaaa gaaagggatt acgtgtcggt cttgtcatgg agtttactga
     2581 aggtgcagac actgcagtat ccaagatgat ctataaagct gcagacacac tggagcagca
     2641 gggctgcaac tgcgaagaag tgtctcttcc gtcagtgcga tatgcgcttg cctcttacta
     2701 tacaatcgca accgcagaag c
//
LOCUS       DASQTF010000198         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_255387,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000198 DASQTF010000000
VERSION     DASQTF010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255387"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..517)
                     /gene="lysM"
                     /locus_tag="VE308_04700"
     CDS             complement(92..517)
                     /gene="lysM"
                     /locus_tag="VE308_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator LysM"
                     /protein_id="HZB00302.1"
                     /translation="MSSNVDETDEKIIRILQADSRKAFVEIANEIGLSESAVRRRVKN
                     LIDRMIIKRFTIELGASDKTSAITLISVASTADTSAVSNQLMNLNGVKVVYEITGQYD
                     IAAIIAAPAITDINKCIDDIRKIEGVSDTDTVIILKTMR"
     gene            complement(507..1688)
                     /locus_tag="VE308_04705"
     CDS             complement(507..1688)
                     /locus_tag="VE308_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HZB00303.1"
                     /translation="MNDEDRYLATLYQRFPVNIAKGKGTRVWDTAGREYIDCMGGYGV
                     ALVGHCNDRVVNAIKRQAETLITAHMSTYNDTRLRFMEKIASVTPPSLNKMFFANSGA
                     ESVEAALKFARKYSGRHGVIAMNGAYHGKTFGALSVTYSEKYRKSFMPLLDGVKFVPY
                     SDPSMLEEVIDDTIGSVILEPIQGETGIIVPPDDLLPKIREICNRLNLVLIFDEIQSG
                     LGRTGKMWAGENWNTAPDIMCLAKGIAGGIPMGLVLAKQEIMDKMKLGEHSSTFGGSP
                     IACAAGLATMEALIDDKLVENAAKMGLHFKEGLNRLKDKHKIIREVRGIGMMLGVELR
                     FDVKDILFDGIRRGLLLLYSGRNILRLLPPLVMDETTVSRAVDIIDVILTDEEKRRNV
                     I"
     gene            complement(1685..2488)
                     /locus_tag="VE308_04710"
     CDS             complement(1685..2488)
                     /locus_tag="VE308_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate/[LysW]-glutamate kinase"
                     /protein_id="HZB00304.1"
                     /translation="MIVIKIGGSVVDGLHSSALADIKSIAEKYKLVIVHGGGREVTAT
                     ATKLGKEQKFIVSPGGVRSRYTDKETAEIYTMVMSGKLNKAIVQMLLRQNINAVGVAG
                     IDGGILKAERKKKLLIINEKGRKMIIDGGYTGKITSVDAALVTDLVSSGYVPVISPIA
                     LSEEFDFLNVDGDRAAAYVAGGIKADKVIFITDVNGLILNEKLVNSIALDQAKAALPK
                     IGSGMDKKVLACIEALEMGVKEAIIASGQVENPLSTAIAHRNCTVIVPY"
     gene            complement(2485..>2866)
                     /locus_tag="VE308_04715"
     CDS             complement(2485..>2866)
                     /locus_tag="VE308_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HZB00305.1"
                     /translation="QIKVSMTPHAVNIVRGILTTNHTFLNRPVEELELWKMYRNAYKR
                     EPFVRLIRDKKGLYKFPDPKFVVGSNFCDVGFDIDEYNQRVVALSASDNLMKGAAGSA
                     IQNMNVMFGYGENEGLMYTPLTPV"
BASE COUNT          697 a          731 c          573 g          865 t
ORIGIN      
        1 ctttatactg gatgctacaa caatattgtg tatttatgga tggggtttgt ccaaatatca
       61 ggtagatatt atcttaggct tatgcaagag cctatcgcat agtttttagt attatgacgg
      121 tgtctgtgtc agacacgccc tctatcttgc gtatatcgtc aatacacttg ttgatgtcag
      181 ttatcgctgg cgctgcaata atagcggcta tatcatattg gccagttatt tcgtagacca
      241 ctttgacgcc atttagattc atcagctggt tagacacagc cgatgtgtct gcggttgagg
      301 ctacggagat tagggttatc gcacttgtct tgtcgcttgc cccaagctca atagtaaagc
      361 gtttaatgat catcctatca ataagattct tgactcttcg ccgaactgct gattcggaaa
      421 gtccaatctc attagcaatc tctacaaatg ccttgcgaga atctgcctgc aaaatgcgta
      481 ttatcttttc gtcagtttca tctacgttag atgacatttc ttctcttttc ctcatcggtt
      541 aatataacat ctattatatc aactgcgcgg gaaaccgtcg tttcgtccat tacaagtggc
      601 ggaagcagcc ttagaatatt tcttccagag taaagaagca gtaatccacg gcgaattccg
      661 tcaaagagta tatctttgac gtcaaagcgg agttctactc ctagcatcat cccaataccc
      721 cttacttctc ggattatctt gtgcttgtcc ttcagtcggt tcagtccttc tttaaaatga
      781 agacccatct ttgcagcatt ctcaaccagt ttatcatcga tcaaagcctc catagtggca
      841 aggccggcgg cgcatgctat agggcttcca ccaaatgtag aagaatgctc accaagcttc
      901 atcttatcca ttatctcctg cttggcaaga acgagcccca taggtattcc acctgctatg
      961 ccctttgcaa ggcacataat atcgggggca gtgttccagt tttcacccgc ccacattttg
     1021 ccggttctcc caaggcctga ctgtatctca tcaaatataa ggacaaggtt gagcctattg
     1081 cagatctctc tgatctttgg cagaagatca tctggaggaa caatgatgcc agtctcgccc
     1141 tggatgggtt caagaattac acttccaatt gtatcatcga taacttcttc cagcatcgac
     1201 ggatcgctgt agggaacaaa cttgacacca tccagtagtg gcatgaacga ctttctgtat
     1261 ttttcgctgt atgtcacaga aagcgcccca aacgttttgc catgatacgc accattcatt
     1321 gctattacac catgtctgcc gctgtacttg cgtgcaaact tgagtgcagc ttcgacagat
     1381 tctgcgccgc tgttggcaaa gaacatcttg ttcaaagatg gaggggtcac tgacgctatc
     1441 ttttccatga atctcaatcg agtatcattg tatgtggaca tgtgtgccgt gatgagtgtc
     1501 tctgcctgcc ttttgatagc gtttaccact ctgtcgttgc agtgcccaac cagtgcaact
     1561 ccataaccgc ccatgcagtc aatgtactcc ctgcctgcag tgtcccagac cctggttccc
     1621 ttgcccttcg cgatattcac tggaaacctc tggtaaagag ttgccagata ccggtcttca
     1681 tcgttcaata tggaactatc accgtacaat ttctatgggc tattgccgta gacagcgggt
     1741 tttcaacctg gccagaagca atgattgcct ctttaactcc catttcaagt gcctctatac
     1801 atgcaagcac cttcttgtcc ataccggacc ctatctttgg cagtgccgct tttgcttgat
     1861 ctaaggctat ggaattgaca agtttttcat ttaatattag accatttaca tctgtgataa
     1921 agataacctt gtctgcctta atgccgcctg ctacatatgc tgcagccctg tcgccatcca
     1981 cattgagaaa atcaaattct tcgctcaacg caatcggcga aatcacaggc acatacccac
     2041 tgctaacaag atcagttacc agagcagcgt caactgatgt tatcttgccg gtatagccgc
     2101 cgtctattat catctttctt cctttttcat tgattatcag aagcttcttc tttctctctg
     2161 ccttcaatat accgccgtct attccagcaa cgcctactgc attgatattc tggcgcaaaa
     2221 gcatctggac tattgccttg ttcagctttc cggacatgac catcgtatag atctcggcag
     2281 tctccttgtc tgtataacgg cttcgcacgc cgccaggaga cacaatgaac ttttgctcct
     2341 ttcccagctt agttgcagtg gcagtcactt ctctgcctcc accatgcaca ataacgagct
     2401 tatacttttc agctattgac tttatgtctg caagagctga ggaatgaagc ccgtctacca
     2461 cgcttccgcc tatctttatg actatcatac tggtgtcaat ggggtataca tcaatccttc
     2521 attttcgcca tatccaaaca tcacgttcat attctgtatc gcagagcctg cagcgccctt
     2581 cattagattg tcagaagcgg aaagtgccac tactctctga ttgtactcat cgatgtcaaa
     2641 tcctacatcg cagaagttgg agccgaccac gaatttcgga tcaggaaact tgtagaggcc
     2701 cttcttgtca cggatcaatc ttacaaaagg ctcgcgcttg tacgcattac gatacatttt
     2761 ccatagctct agctcctcca cgggtctgtt gaggaaggta tggttagtag tgaggatgcc
     2821 tctgacgata ttgactgcat ggggagtcat gctgacttta atttgc
//
LOCUS       DASQTF010000199         3875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_154372,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000199 DASQTF010000000
VERSION     DASQTF010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154372"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            857..1144
                     /locus_tag="VE308_04720"
     CDS             857..1144
                     /locus_tag="VE308_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00306.1"
                     /translation="MQSRHWHAGPDVDPAAVRAGKERYNVGNVFRRAEALQRGQFAKL
                     FNLCVCLIGKEELGRHRAWAAAFTVILRPRSSLASKRTSPSRPALEAIYGP"
     gene            1503..1952
                     /locus_tag="VE308_04725"
     CDS             1503..1952
                     /locus_tag="VE308_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZB00307.1"
                     /translation="MARIHVHTHGKSHSTRPTSKGPAPWLSQNRDQISALVVELAKEG
                     LSPSEIGLRLRDEHGIPLVKPVLGKSVTSVLVENNIKSDMPEDLDKLVRKALGLQKHL
                     RVHNSDHRNVRSLELIESKIHRLSKYYKRERKIPNNWKYAAVIAQLE"
     gene            1971..3413
                     /locus_tag="VE308_04730"
     CDS             1971..3413
                     /locus_tag="VE308_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZB00308.1"
                     /translation="MSDRLTDALKYFCEKLRSTVEGGNEVSIITHLNADGITSGSIMA
                     MALRRMGARYSVRAVSDMNSTVVEKMKADGRDFYVVTDLGAGWASHLRNALGDKWVII
                     DHHEIIEEEILADNEAQILNPWKFGVDGGREVSAGGMAYMVASTLDLKNRDLSAIAVV
                     SALADRQDQGEKKSFFGLNTEILKTAQSLGLLSVDLDIILTGRETRPLHEALAYSLFH
                     YIDGLTGNKEACYLLLKNAGINLKHNGGRWRVLAEFSQEEKSAIVEAVANFVATSDKR
                     ISDILRQELIGYVYTLAGEDKRSQLRDAREFSTMLNACGRVGMAGVGISICMGDRNNA
                     LSAGEELMSMYKMTLRKNISTIFSEKWRLADDGKIIFVNADGILEEGMLGAASTMLSG
                     SPSLPGRLLFVRTLTKDGTYKFSSRKCLDCKSQANLGMIMRLRSKALNGAGGGHSAAA
                     GCSIPSSALEEFIVGIKAEINDPKFASGPS"
     gene            3394..3651
                     /locus_tag="VE308_04735"
     CDS             3394..3651
                     /locus_tag="VE308_04735"
                     /inference="COORDINATES: protein motif:HMM:NF020899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HZB00309.1"
                     /translation="MQADHLNIAVGVEIPFSSSAEAKAAMKALIPDNVNFPKGLSVKM
                     FSRGSTLAIQLTGKNIPTATVLSTLDEILEHISVAKKVMAG"
     gene            3651..>3875
                     /locus_tag="VE308_04740"
     CDS             3651..>3875
                     /locus_tag="VE308_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZB00310.1"
                     /translation="MLDPKILKENPDAVREMLKMRNMTDFPMDELTTLYKLRGDLIIK
                     TQELRRQKNLLSETVAGKKKSKQDASFELGE"
BASE COUNT         1128 a          794 c          944 g         1009 t
ORIGIN      
        1 caaatcttta ctactctcgg gatccaagca gattttactt acaggccagt taaggatctt
       61 attatgcttc agttgctata ttgcctgtag actcctctaa ttagaagaca gtggtcactt
      121 tctatctatg tatttcacaa caaatgttta actatttttg gggccattat gtaatacata
      181 gttatgaaca aaaaagcaga tgttcagtgt gtacatattg ataaagtaaa tccaaaaatt
      241 catggcaata cagaaggatg tgaagaatgt gaaaaaatag gttctggctg ggtgcatctg
      301 cgattatgct taacatgtgg gcatgtagga tgttgcaact cttctgccaa taagcatgga
      361 acgaaacatt tcctagaaac agggcatcct atcataaagt cctatgaacc aggcgaggat
      421 tggaaatggt gttacgcaga tgacatcttt ttggagtgat aggagaagag aatccatatt
      481 ccatatatat gatcgctcct tttagatttt gaaccgaaac caaattcaac gtacttgttt
      541 tgccttcaca tttctccaaa tatcttcacc tgttctctgg cttggcttgg aaggacctgc
      601 gataaaatgc gagacgctcg atggtttttc atcaatatga aatgccaagg cacgttcttg
      661 aatagagcga tcaggaaccg tgatctgttc gtgttgacgt aattgctgac tagggacctt
      721 atcctaactg cacaacgaca ctaggttcaa catagtgaaa gccaatacta tgcttcttct
      781 ttttctattc aaactcagcc ttttgtgtaa ccttttaacg ttcttgaagt gtctacgaac
      841 tcaactaaaa gtcgctatgc aaagccgcca ttggcacgca ggacctgacg ttgatccagc
      901 cgccgttcgg gccggcaagg aacgatacaa cgttggcaat gtcttccggc gggccgaggc
      961 gttgcagagg ggacagtttg ctaagctctt caatctgtgc gtctgcctta tcggtaagga
     1021 agagctcggt cgccacaggg cctgggcagc ggcgtttacg gtgatattgc gaccccgcag
     1081 ctcgttagca agcaagcgga ctagcccctc aagacctgcc ttagaagcga tatatgggcc
     1141 ataggccggg aaagccttcg ctataatact gcttgaaaaa agcgatcaat attatgagca
     1201 tcacaatatg cttgcagggc acttatgata atgtagatac tagagtatga agcccccctc
     1261 tttttagttg tattacgcac tcgatttatt ttcccttgga gtaccttacc ttttgttttg
     1321 ctgtcaatta ggttaaaccc tgtagcctct ttaaattggt caagcgattg tttataagtg
     1381 actctcgtag ctggtgattt tatgctttca aggaacaagt ccccagaagt cgtcgtcatt
     1441 gtcgtcaatg gaggtgtaga gtagaacgac ttatatcagc agtttgttca tactcgttca
     1501 aaatggctag aattcacgtt catactcatg gtaagtctca ttcgacccgt cctacttcaa
     1561 aaggccctgc accctggctt agccagaatc gagatcagat ctcggctctt gtagtcgagc
     1621 ttgcaaaaga aggtttgagt ccaagtgaga taggtctgag gttaagggat gaacatggga
     1681 taccgctcgt caagcctgtt ctaggcaaga gtgtcacgag cgttttggtt gagaataata
     1741 taaagtcaga catgcccgaa gacttagaca agctggtgag aaaggctctt gggctccaga
     1801 agcaccttag ggttcacaac agcgaccaca ggaacgtgag atcactcgag ttgatagagt
     1861 caaagatcca cagactttca aagtactaca aacgagagag gaagatccca aataactgga
     1921 aatatgcagc agtgattgct cagctagagt agtagtaaaa ggtacttcag atgagtgaca
     1981 ggcttactga tgccctcaaa tatttttgtg aaaaattaag atcaactgta gaaggcggca
     2041 atgaggtttc catcatcaca catcttaatg cggatggaat cacatctggt agcataatgg
     2101 caatggcact cagacgaatg ggtgcacggt attctgtcag ggcagtctct gacatgaatt
     2161 cgactgtagt tgagaagatg aaagctgacg gccgggattt ttatgtcgta actgacctag
     2221 gagcaggttg ggcgtcacat ctaagaaatg ccctgggcga caaatgggtc atcattgacc
     2281 accatgagat tattgaggaa gagatattag cagacaatga ggctcagata ctaaatccat
     2341 ggaaattcgg cgttgatggg ggaagagaag tatcggcagg gggaatggcg tacatggtcg
     2401 caagcacgct tgatcttaag aaccgtgacc tgtcggccat cgcagttgtt tcagcactag
     2461 ctgataggca agaccaagga gaaaagaagt cattttttgg tctaaatact gagatattaa
     2521 agacagccca gtcgcttgga cttttaagcg ttgatctcga catcatacta actggaagag
     2581 agacgcgtcc gctacatgag gcgcttgcat actcattatt tcattatatt gacgggctca
     2641 ccgggaacaa ggaggcctgt tacctgttgc tcaagaacgc tggcataaac ctcaagcata
     2701 atggtggccg ctggcgcgtg ctggcagaat tttcacagga agaaaaaagc gcgattgttg
     2761 aagcagtagc caactttgtg gctacttcgg ataagagaat atccgatata ttgcgacagg
     2821 aacttatcgg ttatgtctac acgcttgcag gagaggacaa gcgaagccag ctaagagacg
     2881 caagggaatt ttcaacaatg ctcaatgcat gcgggagggt aggaatggca ggagtcggta
     2941 tttcaatttg tatgggagat agaaataacg cattaagcgc aggagaagaa ttaatgagta
     3001 tgtataagat gactctgcgc aagaatattt ccaccatctt tagtgagaaa tggaggcttg
     3061 ccgacgatgg caagatcatc tttgttaatg ctgacggcat tttggaagaa ggcatgttgg
     3121 gagcggcgtc aacaatgctc agtgggtcac cctcccttcc gggtaggcta ctatttgtcc
     3181 gaaccctcac aaaggatggg acctataagt tctcatcaag gaagtgcttg gattgcaaat
     3241 cgcaggcaaa cctcggcatg ataatgcgac tgcgctccaa ggcattaaat ggtgcggggg
     3301 gaggacattc agcagctgct ggctgcagca ttccctcttc tgctcttgag gagtttattg
     3361 tgggcataaa ggcggagata aatgatccga aatttgcaag cggaccatct taatatcgca
     3421 gtaggagtcg agatcccttt ttcgagcagc gcagaagcaa aggcagctat gaaggcatta
     3481 ataccagaca atgttaactt tccaaagggt ctgtctgtga aaatgttttc aagaggctct
     3541 acacttgcaa ttcaattgac aggcaaaaat attccaactg caacagtatt gagcaccctt
     3601 gacgaaattc ttgagcatat atcggtagca aagaaagtga tggccggcta gtgttagacc
     3661 cgaaaatttt gaaagaaaac cctgacgcag ttcgagaaat gctcaaaatg cgtaacatga
     3721 cagatttccc aatggacgaa ctgacaacgc tatacaagct ccgaggtgat cttattatca
     3781 agactcagga gcttcgtcga caaaagaacc tgctttctga aaccgttgca ggcaagaaaa
     3841 agtccaaaca agatgcctct tttgagcttg gtgag
//
LOCUS       DASQTF010000200         8532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_448568,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000200 DASQTF010000000
VERSION     DASQTF010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448568"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /gene="truD"
                     /locus_tag="VE308_04745"
     CDS             <1..750
                     /gene="truD"
                     /locus_tag="VE308_04745"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HZB00311.1"
                     /translation="LQRFGSERLVTHLVGREIVKRNFIQAVELLLSHTTEFDTQMSRE
                     IRNRCADPNNYRQLLKILPRGMDIERQVLSALVAGRDTIAALRAIPITIRRLFVQAYQ
                     AYIFNRCLSRAIMRDEDLLKPQTGDLCFEMEGPATFGRIIKYDPNSRTELVPAIRMAG
                     YTFQPSKGRFENITRAILQEEEVAAKDFYIKEMQELSQQGGFRQAPLWCMDFWYKRDP
                     LMVSFKLPKGSYATTLLREIIKPDDPIRSGF"
     gene            1402..2055
                     /locus_tag="VE308_04750"
     CDS             1402..2055
                     /locus_tag="VE308_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HZB00312.1"
                     /translation="MLGGRNLHEIIEDAIDDIYENLSVKIDKTNSDRIHAIEASSCTR
                     LAYYERKDSLPSDNAAKISSLLGNGMRHSLSNVRGEYKIDTLALEVNVDMIMANEFIV
                     RFEIVPTLPEVPHPQHMLYLNACLFAFNKDEGFLIYMTEDGKMVEFSVTKNNRMFEEL
                     VRRARVLSTLLKESRVPIVEPSNLCTTCKYFERCYARKKVKDESSGFILEEIFKPKK"
     gene            complement(2084..2218)
                     /locus_tag="VE308_04755"
     CDS             complement(2084..2218)
                     /locus_tag="VE308_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00313.1"
                     /translation="MAEETFMFAVTAGVFVFLVAVLFGSRARRYSQGYMPSEGHSEHS
                     "
     gene            complement(2293..2841)
                     /locus_tag="VE308_04760"
     CDS             complement(2293..2841)
                     /locus_tag="VE308_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00314.1"
                     /translation="MNTKAIYAVAGGVAIAAVAIFFIIGMGNFRPLGNSEDPGTSQTQ
                     IADLQLALRDIMVEKTNEENANVQIVFDMHNPNRSTAILETIHYTLDVGQYQMAIGDI
                     GVSPEGFVSGQEDIFPIVGSSTVTLRDTQVAVRNNLTASSWDSMVDGTGQYRVEGMYS
                     YRLTGSNFQTNYFEKEFTLTFP"
     gene            2927..3244
                     /locus_tag="VE308_04765"
     CDS             2927..3244
                     /locus_tag="VE308_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00315.1"
                     /translation="MQYHKAANIGRERAHKAQMRLFDYTGFAMLTYTIKQGVAGFEPV
                     GEEELVGKMTSGNESMLFICDKDGYAKAQSRPMPMEQGEEVFKRMLADGMQEFSGKIK
                     TVS"
     gene            complement(3455..4156)
                     /gene="pyrH"
                     /locus_tag="VE308_04770"
     CDS             complement(3455..4156)
                     /gene="pyrH"
                     /locus_tag="VE308_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZB00316.1"
                     /translation="MWGRVRFTQARHRVVIKLSGKVFGDETAYELKRYASMMSKISKK
                     VQLVVVAGGGKVARHYISIARSFGSDEASLDIIGIEVSRLNARLLILALGDQAYPAVP
                     EDLEQVSKAVTTNRIVVTGGLHPGQSTNATAALIAEKVKASKFLNATDVNGIYDSDPT
                     KNKNAKLFNEITVKECIDLLGSEDSAAGGYDLMDIVALKVIERSKIPTIVLKSDPAVI
                     KNAISRSTAGTRIIV"
     gene            complement(4125..5390)
                     /locus_tag="VE308_04775"
     CDS             complement(4125..5390)
                     /locus_tag="VE308_04775"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZB00317.1"
                     /translation="MDFAGEITDPIHKYIRFSHVEKQLIDSPAFQRLRRIRQLAGAHL
                     VYPSAQHSRFEHSLGAMHIAGLAGETLLGKEYIDHTEVVQDLRLAALLHDIGHGPFSH
                     LFEEVLEYHCNTSHEELGKKIIMQSEISDILERYGYSAHQICRLSFGQSKVNFMNEII
                     SGGLSADIMDYLPRDGLFTGAEYAKLDYHRLVSSLEVSKNRLAINKSALNSLESMLIS
                     RYEMFKAVYFHKTVRSAEVMLLRSMISADEALGLTNTSLHNYLALTDEVTLERLCALS
                     GRYSFSGKMARDYRDRRLLKSVYEKFLHKRDKQRIDRKALEALASHIADVADVDTNYV
                     FVDVSRAPSMPLTPSKEEMYSVLIVDKDRVYEMPVSEIPLVESISGFFDMLRVYTTAE
                     NREKVERAIKKVLGDQGILNMGAGKIHAG"
     gene            complement(5427..6020)
                     /gene="tmk"
                     /locus_tag="VE308_04780"
     CDS             complement(5427..6020)
                     /gene="tmk"
                     /locus_tag="VE308_04780"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZB00318.1"
                     /translation="MATRGKIIVIEGTDKAGKTSQSRMLAETLKASGKVCVILDFPDY
                     TTPIGMEIKAFLEGKRDYLSEVKHLLFSANRWEKKKEIESMLENGTIIVMNRYWQSNL
                     VYGAANGMDTNWLLRLDKGLPKEDIVLVILVDPSISANRAETQDVFESDSQLAAKAYK
                     NYLKFAKQYRWKVVDGSKSKEQVHQELTKMIRKELKV"
     gene            6277..6948
                     /locus_tag="VE308_04785"
     CDS             6277..6948
                     /locus_tag="VE308_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00319.1"
                     /translation="MKKSTIAIFDTFKTRKNKFTGEAKRQRGIIAHLATEQSPDLRTR
                     TSIAHAIAKQHGILWQNIYSGIFRDLDEVLIPAGVVREGGRLPLRRGPKALQLEGVPF
                     YELTDTGILVASSIEELSNNRMQLLQSYISSLSASDSDSKIMKEGMLFLILVAPSFVS
                     KIINEYIYAYSTGTIDSIAPLDIKKLRSVISKQISVEKELVEAVITMQQDQREIVTNF
                     FKVLT"
     gene            complement(6945..7127)
                     /locus_tag="VE308_04790"
     CDS             complement(6945..7127)
                     /locus_tag="VE308_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HZB00320.1"
                     /translation="MYAGSYEMPNCNACGRQVRPGELSESFSCPKCRNATIWRCEGCK
                     GKQKKYHCTACGYKGR"
     gene            7242..7616
                     /gene="hsp14"
                     /locus_tag="VE308_04795"
     CDS             7242..7616
                     /gene="hsp14"
                     /locus_tag="VE308_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp14"
                     /protein_id="HZB00321.1"
                     /translation="MERGLAKELDSRCREFYEFVMPTIDIIEEGDDLMVTVDLPGFVK
                     KDINLRIVGNVLYITAKREQVEALGTVYYRHSPLKIDKKVLLPISFKEEEEEKVEGKP
                     TYENGIVTLRSPVPKSTNIPIT"
     gene            complement(8002..>8532)
                     /locus_tag="VE308_04800"
     CDS             complement(8002..>8532)
                     /locus_tag="VE308_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HZB00322.1"
                     /translation="RYIVERGIALSIQSMHGRQVDRMEVKALVDLANKTMSRFPFRLP
                     KNLALYMRMSSILEGIYYHHKVSFQFVRVLANLLEEEGLLRDAYIEEVKISAKRFAKG
                     IQDSVSVGPLLKTYLESIGPYGRQEKNNGALAASILASALFIGSALMLPYNFYLAYGG
                     FAASAAAVAVVLKKKI"
BASE COUNT         2454 a         1903 c         1845 g         2330 t
ORIGIN      
        1 ctccagagat tcggcagcga aaggttggtg acccatcttg ttggtaggga aattgtcaag
       61 cgcaacttta tacaggcagt cgagctactg ctctcccata ctactgaatt tgatactcag
      121 atgagcagag aaatcagaaa taggtgcgct gacccaaaca attatcggca attactcaag
      181 atactgccaa gaggcatgga cattgagcgt caggtgctct cagcgttagt cgccggcagg
      241 gataccattg cagcactgcg tgcaatacca atcacgattc ggagactatt cgtccaagca
      301 tatcaagcgt atatcttcaa cagatgtctc agcagagcta tcatgcgaga cgaggatctg
      361 ttgaagccgc agactggaga cctttgcttt gaaatggagg gacctgcgac ctttggtaga
      421 ataatcaagt acgatcctaa ctcaaggaca gaattggttc cagcaataag aatggcagga
      481 tataccttcc aaccaagcaa gggcagattt gaaaatatca cgagagcaat actgcaggag
      541 gaagaagtag ctgcaaagga cttttacata aaggagatgc aagagctgag ccaacaagga
      601 ggctttagac aggctccact ctggtgcatg gatttttggt ataaacgaga tccactgatg
      661 gtatcattca agctgcctaa aggatcgtat gcgaccacac ttttacgcga aataatcaag
      721 ccagatgatc caatcaggag tggcttttag ccattcaaat catgttgcaa taacagcaaa
      781 aaatcccttt atttaatgat gctaagataa gcattgccgc cagatagttg aacaacattt
      841 ttcctttcac tattcaggat tatttgtcca tagttatggt tcttagtata ttatttattt
      901 tatcacatga agctacacat attgccaatg tgtgggaatt tttaaatata gagaggactg
      961 caggatgcag ctgataaaag cccaccttac tatgattata gactattccc ccctcacaca
     1021 tttgatcgat cgacatgaaa aatcaagata cctgaaaaag ccatacacat atttctccaa
     1081 tcttcacctt gacaataaag cataggggtt tggcgatttg tatcagcttt tgaattctac
     1141 atttgtacag tagcaaaaga ggctcttttg cagtaagatt acctcggatt tggaattgaa
     1201 acacgggata catgccaata cacaggcaat ttcaactatg ctcgttacta ttcatcccct
     1261 tgcgaataac aatctttgca cgaagtgata gagactccgc ctttgttttc aacagaaaaa
     1321 tatactcttt ctaatacaaa actgtaaata aaaaaggctc aatataatat atagcacggc
     1381 agcatagtac actgtattca tatgctcggt ggcaggaatc tgcatgaaat aatagaagat
     1441 gccattgacg acatatacga gaacctgagc gtcaagatag acaaaacgaa ctctgacagg
     1501 atccatgcaa tagaagcatc aagctgcaca aggttagcat actatgaacg caaagactcg
     1561 ctaccttcag ataatgcagc taagatctca agcctgcttg gaaatggtat gcgccactca
     1621 ctgagcaatg tacgcggcga atacaagatc gacacccttg cactcgaagt taacgtagac
     1681 atgataatgg ccaacgagtt tatagtacga tttgaaattg tgcctacact gccagaggtt
     1741 ccgcacccac agcacatgct ttacctcaat gcatgtcttt ttgcattcaa caaggacgag
     1801 ggttttttga tctacatgac tgaagatggc aagatggtcg aattctctgt gaccaagaac
     1861 aataggatgt ttgaagaact cgtcagaagg gcaagggtgc tcagcacatt gctaaaagaa
     1921 agtagagttc caatagttga gccatccaac ttatgcacaa cttgcaaata ctttgaaagg
     1981 tgttatgcaa ggaagaaggt aaaagacgag agttccggct ttatactgga agaaatcttt
     2041 aaacccaaga aataagaaat gatgaaaaca tagaaacgtg cagtcagctg tgctcgctat
     2101 gaccttcaga cggcatatat ccttggctat accttcttgc ccttgagcca aacagcacag
     2161 ctactaggaa tacaaatact cctgccgtca cagcaaacat gaatgtttct tcagccatat
     2221 tatggagccc tgcatgaaac ttaatacctt tatgaattgc atcataacat gctatgataa
     2281 tatcataaaa tgtcagggaa atgtcaatgt aaactctttc tcaaaatagt ttgtttggaa
     2341 gtttgagcca gtcagcctgt aggaatacat cccctcaacg cggtactggc cagtgccatc
     2401 taccatgctg tcccagctgc tggcagtaag gttattacgc actgcaacct gtgtatctct
     2461 gagagtgacg gtgctgctgc caactatcgg aaatatgtcc tcttgcccag atacaaaacc
     2521 ctcagggctt actccaatat caccgattgc catctgatac tggcccacat ccagtgtata
     2581 gtggatcgtt tcaagaatag cagtgcttct gttagggtta tgcatgtcaa agacgatctg
     2641 tacatttgcg ttctcttcgt ttgttttctc aaccataatg tcccggagtg ccagttggag
     2701 gtcggctatc tgggtctggg acgtgcctgg atcctcgcta ttgccaagag gcctgaaatt
     2761 tcccattcct ataatgaaaa atatggctac agcagctatt gcaacaccgc cagctactgc
     2821 ataaatggcc tttgtattca caattgcctt tttcgcgttc gcccacgctt taaagctgtc
     2881 gattaatcca aatccttata ctagtcgtaa aaaaaattgc taccggttgc aataccacaa
     2941 ggcggcgaat attggacgcg agagggctca taaagcccag atgaggctgt tcgactacac
     3001 cggctttgcc atgctgacct atactattaa acagggtgtg gcagggtttg agcctgtggg
     3061 ggaagaagag ctggttggta aaatgacgag tggtaatgag tcaatgcttt ttatttgtga
     3121 taaagatggc tatgctaagg ctcagtcaag gccgatgccg atggaacagg gagaagaagt
     3181 tttcaaaagg atgcttgcag atggtatgca agagttctct ggcaaaatca agacggtatc
     3241 ctagctataa tatgctaatt tgaactttcg agaaaaagac agaaggtggt agtggtgtgg
     3301 tgtgtgttgt aaaggtcatc gtcattagac agggttgaga tgtagctcaa tacacctaac
     3361 ctatagcaag attggatgat gcaaatagaa gctattatta ttttagggcc ttgtcactag
     3421 tttgccaaag cagaatatat gaatttgata atgcctatac tatgatgcgt gtccccgcag
     3481 tgctacggga aatcgcattc ttgattactg cgggatctga cttgagcact atcgtaggga
     3541 tcttgctccg ctctattacc ttgagcgcca caatgtccat cagatcatag ccgcctgccg
     3601 ccgaatcttc agatcccaat agatctatac actctttgac agtaatttcg ttgaacagct
     3661 tggcgttctt gttcttggtg ggatcggaat cataaatgcc gttaacatct gttgcgttga
     3721 ggaacttgga agctttgacc ttttcagcaa taagtgcagc tgttgcattt gtgctctgcc
     3781 ccggatgtag accgcctgtc acaactattc tgttggttgt tacagcctta ctcacctgct
     3841 ccaagtcttc tgggactgca gggtacgcct gatcgccaag cgcaagaatc aggagtcttg
     3901 catttagcct tgaaacctcg atgcctatta tgtccagaga tgcttcatcc gagccaaagc
     3961 ttcttgcgat ggagatgtag tggcgcgcaa ccttgccccc gccagctaca accactagct
     4021 gcactttctt gcttattttg ctcatcatac tagcatacct ctttagctcg tatgctgttt
     4081 catcgccaaa cacttttcca gataatttga ttacaacccg atgcctagcc tgcgtgaatc
     4141 ttacccgccc ccatattcag tatcccttga tcacccagta cctttttaat agctcgctcc
     4201 actttctcac ggttctctgc agtagtatag acgcgcagca tgtcaaagaa tcctgagatc
     4261 gattctacaa gtggaatctc tgacactggc atctcatata ctcgatcctt atcgacaatc
     4321 aatacagaat acatctcttc cttgcttggt gtcagcggca tcgagggtgc acgagagaca
     4381 tcgacaaaga catagttggt atcgacatct gccacatctg caatatgaga agcaagcgcc
     4441 tccaacgctt ttctgtcaat tctttgctta tcgcgcttgt gcaagaattt ttcgtaaaca
     4501 ctcttaagca gcctcctgtc gcggtaatct cgcgccatct tgcccgaaaa tgaatatctg
     4561 cctgaaagcg cacagagccg ctcaagtgta acttcatccg ttagcgcaag atagttgtgg
     4621 agcgaagtat ttgtcagtcc aagtgcttcg tctgcagaaa tcattgagcg caggagcatt
     4681 acttcagcag atctcacggt cttatggaaa tagactgcct tgaacatttc atagcgtgag
     4741 atcagcattg attcaagtga gttgagagca gacttgttta tcgcaagccg gttctttgaa
     4801 acttcaagcg aactcacaag ccggtggtag tcaagcttgg catactctgc tccagtgaaa
     4861 agcccgtcgc gtggcaaata gtccatgatg tctgcagaca gtccaccaga aatgatctca
     4921 ttcatgaaat ttacctttga ctggccaaat gaaagccggc atatctgatg tgcactgtag
     4981 ccgtatcttt ccagaatgtc agatatctcg ctttgcatta tgatcttttt tccaagctcc
     5041 tcatggctgg tattacaatg atactcaagg acctcttcaa acaggtgcga aaaaggccca
     5101 tgcccaatat cgtgtaacag cgcggcaagc ctgagatcct gtaccacttc ggtgtgatct
     5161 atgtattcct tgccgagtaa cgtttcgcct gcaaggccgg cgatatgcat tgcgccaagt
     5221 gagtgctcaa agcgtgagtg ctgggcgctc gggtaaacca gatgtgcgcc agccagctgt
     5281 cttatcctcc gcaagcgctg aaaagcaggg ctatcgatca gttgtttttc aacatgcgag
     5341 aatctaatgt acttatggat agggtctgtt atttcacctg caaaatccat attgattgct
     5401 tgttttgtat cacagcctaa caggacttaa actttaagct ctttccttat catcttcgtt
     5461 aattcttggt gaacctgctc cttgctcttt gagccgtcaa cgaccttcca tctgtactgt
     5521 tttgcaaact ttaggtagtt cttataggct tttgccgcca gctgagaatc ggactcaaac
     5581 acgtcttgag tctctgcgcg attggcagat atgcttggat cgaccaaaat cacaagcaca
     5641 atatcttcct ttggaagtcc cttgtcaagt cgaagaagcc aattagtgtc catgccattt
     5701 gccgcgccgt agacaaggtt tgactgccag tagcgattca ttactataat cgtaccattt
     5761 tcgagcatgc tctcaatctc tttctttttt tcccacctgt ttgctgagaa caacaggtgc
     5821 ttgacctctg agaggtaatc acgcttaccc tcaaggaacg cttttatttc catcccaatt
     5881 ggtgtagtgt aatctgggaa gtccaaaatg acacaaacct tgccggaagc cttcagggtc
     5941 tctgcaagca tacgagactg gctagtcttg ccagccttgt cggtaccttc aattacgatt
     6001 atcttgcccc ttgttgccac tgcctaaggt cgggcatgca tcaataaaaa gattcaaaga
     6061 tctttacctt cagcataaaa tttatgatta aatgtacaaa aatggctcaa ggctaggata
     6121 attacctaaa gaaaatatat caagcttatt tggaagaggc gtgcgtttgt gaatattata
     6181 tggcatgcct gctgaaacat aatctagaaa aacaacaaat taaaatatta acagactgct
     6241 ataaaaatta gccttgcgga tggtgcttgc ttgcaagtga aaaagtcaac tattgcgata
     6301 tttgatactt ttaagacccg caagaacaag ttcaccggcg aggcaaagag gcagcgcggc
     6361 atcattgcac atttggccac agagcagagt ccagatcttc gcactaggac gtcgatagca
     6421 catgccattg ccaagcagca tggcatctta tggcaaaaca tctactccgg catattccgc
     6481 gaccttgatg aagttctcat tcctgcaggt gtggtaaggg aagggggcag gctacccttg
     6541 aggaggggac caaaggcact ccagctagaa ggagtgccat tctacgaact gacagatact
     6601 ggcattttgg tagcatcatc aattgaggag ctgagcaata atcgtatgca gctactgcag
     6661 tcatacatat catcactttc agcaagtgac tctgattcca agataatgaa agagggtatg
     6721 ctatttctaa tattggtggc gccgtccttt gtttcaaaga tcatcaatga atacatttac
     6781 gcctacagca ctggcacaat tgatagcatt gcgccgcttg atatcaagaa gcttaggtca
     6841 gtcatatcta agcagatatc agtcgaaaaa gagttggtag aagctgttat tacgatgcag
     6901 caggaccaaa gagagattgt tacaaacttt ttcaaggtgc tcacttagcg acccttatag
     6961 ccgcaggcag tacagtggta ctttttctgc ttgcccttac agccctcgca acgccatatt
     7021 gttgcgttcc tgcattttgg acagctgaaa ctctctgaca gctcccccgg ccttacttgt
     7081 ctaccacagg cgttgcagtt tggcatctcg taagaaccag catacattga cttgggtgca
     7141 tatcctcctc ggagtatttt tactttgtta aattttgagg ttggaaatca agggatttat
     7201 atcatcttgg cgagatagtt atgctagcag attggagata gatggaaagg ggcctggcca
     7261 aggagctaga tagtagatgt agagaatttt acgagtttgt catgccaaca attgacataa
     7321 tagaagaggg cgacgatctg atggtcaccg tcgatctccc tggctttgtc aagaaggata
     7381 ttaacctcag aattgtcggt aatgttttgt acataactgc caagagagag caagtagagg
     7441 cccttggcac ggtgtactac aggcacagcc cgctcaagat agacaaaaag gttctgttgc
     7501 caatatcttt taaagaagaa gaagaagaaa aggtggaagg caagccgacg tacgaaaatg
     7561 gcatcgtaac actcaggagt cctgtaccga aatcgacaaa cattccgata acataaatgc
     7621 atacttaatt tttttaatac ttttcaacta tagtaagatt ctgaaattga aagtgaaaga
     7681 agggattgca acctaattga taacaggatt gtgatttacc acatgtaata ctcatcatat
     7741 aactattgca tcattccatt atgccccaga tgtgtcgctt caccgcatca tattgcaata
     7801 gcgataggaa ttttatgtaa tgctacacaa tacggacttt agcagtgctt atgattcgat
     7861 aaaagaaagt ttcaacagct caaatggaag tgtgataatt gcataaaaga agcaaaagca
     7921 cacaatgaaa attagatttt gttgatactg aggtcatgct atatcctagt tcagcagcta
     7981 ctttcccagt atgcatatgg tctaaatttt ctttttcaat accactgcaa ctgccgcagc
     8041 agatgcggca aaaccgccgt acgcaagata aaagttgtat ggcaacatca aggcagaacc
     8101 aatgaacaat gcagaagcaa gtatgcttgc ggcaagtgca ccattatttt tctcctgcct
     8161 gccgtaaggt cctattgatt caaggtatgt ctttaacagc ggcccaacac tcaccgaatc
     8221 ctgaattccc tttgcaaacc tctttgcaga tatttttact tcctcaatgt aggcgtcacg
     8281 taaaagtccc tcttcttcaa gcagatttgc aagtaccctg acgaattgga atgacacctt
     8341 atggtggtag tagatgccct caagaataga agacatgcgc atatagagcg caaggttctt
     8401 tggcagtcgg aaaggaaatc ggctcatcgt cttgtttgca aggtctacca gagcttttac
     8461 ctccatcctg tcgacctgcc gaccatgcat ggactggatg ctgagtgcaa tgcctcgctc
     8521 aactatgtaa cg
//
LOCUS       DASQTF010000201         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_890202,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000201 DASQTF010000000
VERSION     DASQTF010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890202"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..787
                     /locus_tag="VE308_04805"
     CDS             173..787
                     /locus_tag="VE308_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00323.1"
                     /translation="MEVVIGDVRIVGGWVNEPPLVNQLNGIVLIITQVSNGQPVNNAL
                     AQLDTTLQKGTETRLLDFQPTEEAGSYTATILPTQTGQYSVVMRGTIAGQAIDRQIEI
                     EDVEDTARFSFPPAASTGGSGNQIPQGLIDQLQTVITDLTAQVEEANVVAQQANNATQ
                     SVTESIEELRISADRAYLFSLIGVGAGAAGIAVGVVALSRREKI"
     gene            complement(784..1317)
                     /locus_tag="VE308_04810"
     CDS             complement(784..1317)
                     /locus_tag="VE308_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00324.1"
                     /translation="MARSIFFFLTALMYGVIFSVTTTSTTAVYAQDGGMVKTTSKGTL
                     DIRLAPTWSEGGQAAFTVSFLSPGTETVHEHQDYDFRILKDGQQVFSAANQTGQAVLH
                     NVEGTLTVPYTFQENGDYTIQVYLAGIGLPAIPSDEETTFPITVVPEFPAGGILAIVV
                     AASMTTAIVLSQRLKLV"
     gene            complement(1323..1775)
                     /locus_tag="VE308_04815"
     CDS             complement(1323..1775)
                     /locus_tag="VE308_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00325.1"
                     /translation="MSGTLPFQPISAQEQIRLEAVTDQGTFQVEIIWISDDIGSANMF
                     EIHFVDPDTGTEIEDIKYDISIYRDDRPEIQRLDQISIFQEFFFEEVGSYEIRIDNIE
                     DLGERATLPIQVTPEFQHEVFVLSAMALGIAMFAARANGNNLFRHAIH"
     gene            2051..2992
                     /locus_tag="VE308_04820"
     CDS             2051..2992
                     /locus_tag="VE308_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZB00326.1"
                     /translation="MRIVSFLPSATETLYELGAGNQIVGVTHECIYPSQATKKPRVIR
                     SSFDPGQMTGRDIDNKIAELMHSGKDIYIVDENILKRVSPDLIVAQGLCEVCSPFTKE
                     INRAVSLLGDRRPDVLVLDPHDLDDILVSIMDVAEKIGKVREGRKLVAALQKRIDIVR
                     NNNNMKIKRRPKVLCVEWIDPLFTAGHWVPQMVEYAGGINGLSSAGEPSRRMGIDEAV
                     QLDPDTIVLMPCGFDIEQTLKELPTLARNKKWKSLQAVKNGNVYAVNANAYFSKPGPR
                     TIVGLEIISRILHPETSRRIRVPKGSYKKINAKKLLG"
     gene            complement(2981..3094)
                     /locus_tag="VE308_04825"
     CDS             complement(2981..3094)
                     /locus_tag="VE308_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZB00327.1"
                     /translation="MRIHRRVIDVGADDRAIRQLMRLKIPDDVYIEVSLTQ"
BASE COUNT          898 a          704 c          705 g          802 t
ORIGIN      
        1 caagtactat atttgactct tgcttgtttt gtagtacaaa gcccgaaata tacctaaaga
       61 ttataaagat tgtaagagaa atttttgttg aatggagaaa gttgctctat ttttggcagc
      121 cctactactt ttatcgtttc tcgctttcaa ccctgcgcct gtgctcgcac atgtggaagt
      181 ggtaatcggc gacgtcagga ttgtcggcgg ctgggtaaat gagcctcctc tcgtaaacca
      241 gctcaatggt attgtcctga taataacaca agtatcaaat ggccagccag tcaacaatgc
      301 gcttgcacag ctagatacaa ccctgcaaaa gggcacagag accagactcc ttgactttca
      361 gcctactgaa gaggcaggct catacaccgc cacaatactt cccacccaga ctgggcaata
      421 ttcagtagtc atgcggggga caatagctgg acaggcgatc gatagacaga ttgaaattga
      481 agatgttgag gacactgcac gcttttcctt cccgccggca gcaagcactg gcggtagcgg
      541 caaccagata ccacaaggtt tgatagatca gttgcagacg gtcataaccg acttgactgc
      601 tcaggttgag gaggctaatg ttgtggccca acaggccaac aacgcaaccc aaagtgtcac
      661 tgaatccatc gaagaattga gaatatcggc tgatagggcg tatctcttta gcttaattgg
      721 agtaggagcg ggcgctgcgg gcattgcagt cggagttgtc gctctctcgc gcagagaaaa
      781 aatttagacg agtttgagtc tttgtgaaag cactatggca gtcgtcatgg atgcagcaac
      841 gacaatagcc agaatgcctc cagcaggaaa ttccggtact actgttatag gaaatgttgt
      901 ttcttcatcg ctaggtatcg cgggtagacc tattccagca aggtacacct gtatcgtgta
      961 atctccattt tcttggaatg tataggggac tgtcaatgtg ccctcgacat tatgaagaac
     1021 agcctgtcct gtctgatttg ccgcgctaaa aacctgttga ccatccttca atatcctaaa
     1081 atcataatct tggtgctcat ggacggtttc tgtaccgggg ctgaggaagc tcacggtgaa
     1141 cgcagcctgc ccaccctcac tccacgtcgg tgctaatcgg atatcaagtg tacccttgct
     1201 ggtggttttc accataccac catcttgagc atagacagca gtagtgctag tagtagtaac
     1261 gctgaaaatt actccataca tcaacgcagt caagaaaaag aatatacttc ttgccattgc
     1321 ggttaatgga tagcatgcct aaataaattg ttaccatttg ccctggcagc gaacatcgct
     1381 atgccaagtg ccatggcaga gagtacaaac acctcatgct gaaactcagg tgtaacctgt
     1441 ataggtaatg tggcccgctc gcccaaatct tcaatattgt ctatccttat ttcataggat
     1501 cccacctctt caaagaaaaa ctcctgaaaa attgatattt ggtctaaccg ctgaatctca
     1561 ggtctgtcat ctctgtaaat tgaaatatca tacttgatgt cttcaatttc ggtgcctgtg
     1621 tctgggtcaa caaagtgtat ttcaaacatg tttgcactgc caatatcatc tgatatccat
     1681 attatttcaa cctggaatgt accttgatcg gtaactgctt caagcctgat ctgctcctgt
     1741 gcagatatag gctgaaaggg tagagtgcct gacaacaatg caagtgacaa aataacagcg
     1801 atgatgatga taataataat aataattgtt gtgagaacaa cactacgcaa aggaacttga
     1861 tatgaaaaag cttccggata ataatctatt gtgaattaat gcctgctggc tactttctgg
     1921 taccatctag catgaacttg ctctgatctt tagaaagata gccccatcac tggacctatt
     1981 caagaaaatt caagataata atctggccag tcaggttttt aagagatgaa atttttttac
     2041 agtgtatcat atgcggatcg tctcatttct tccaagtgca actgagaccc tatatgaact
     2101 cggtgccgga aaccagatag taggtgtgac acatgagtgc atatatccat ctcaggcaac
     2161 gaaaaaaccc cgggtcatac gatcctcttt tgacccgggc cagatgacag ggcgagatat
     2221 agataacaag atcgctgagc tgatgcactc tggaaaggac atttacatcg tagacgaaaa
     2281 catcttgaaa agagtctcgc ctgatcttat tgttgcgcag gggctttgcg aggtctgctc
     2341 gccgtttaca aaagagataa acagagcagt aagcctcctt ggagaccgcc ggccggatgt
     2401 gctagttctc gacccacatg atcttgatga tatacttgtc agcataatgg atgtagcaga
     2461 aaaaataggc aaggttaggg aaggacgaaa acttgtagct gctctgcaaa agcgtatcga
     2521 catagtgcgc aacaacaaca acatgaagat aaagcgcaga cccaaagtgc tttgtgttga
     2581 gtggatcgac cctcttttta ctgcaggaca ctgggttcct cagatggtcg aatatgctgg
     2641 cggcataaac ggactaagct ccgctggcga gccgtcacgc cggatgggca tagacgaagc
     2701 agtacagctg gatccagata ccattgtgct tatgccatgc ggctttgaca tagagcaaac
     2761 cttgaaagaa ctgcctacgc ttgcccgcaa caaaaagtgg aagtcgcttc aagcagtcaa
     2821 aaatggaaat gtatacgctg tcaatgcaaa tgcgtatttt agcaagccag gacctcgtac
     2881 catagtgggt cttgaaataa tatcaagaat tttgcacccc gagacttcgc gacgcatcag
     2941 agttccgaaa ggttcttaca aaaaaattaa tgctaaaaaa ttattgggtt agcgagactt
     3001 cgatatacac atcgtctggg attttcaaac gcatcagctg ccggatggcc cggtcatcgg
     3061 ccccaacatc gatcacgcgc ctatgtatac gcatttcgta cttgtcata
//
LOCUS       DASQTF010000202         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1259253,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000202 DASQTF010000000
VERSION     DASQTF010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259253"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..104)
                     /locus_tag="VE308_04830"
     tRNA            complement(31..104)
                     /locus_tag="VE308_04830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68..70),aa:Ile,seq:gat)
     gene            194..502
                     /locus_tag="VE308_04835"
     CDS             194..502
                     /locus_tag="VE308_04835"
                     /inference="COORDINATES: protein motif:HMM:NF003084.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HZB00328.1"
                     /translation="MGKKKVLSESELKEMVMPQEGELLGRVIKLVGGDNIIVKCTDGK
                     VRTCRIRGKIKRRMWIRDADLVLVAPWDFQSQRADIIWRYISAHAEKMKADGLLQGLD
                     "
     gene            760..854
                     /locus_tag="VE308_04840"
     tRNA            join(760..796,818..854)
                     /locus_tag="VE308_04840"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:794..796,aa:Ala,seq:tgc)
     gene            complement(1075..2595)
                     /locus_tag="VE308_04845"
     CDS             complement(1075..2595)
                     /locus_tag="VE308_04845"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZB00329.1"
                     /translation="MVSLITPMEIIAAFAVIILAVANFTIIDAQQQLLPTNQPPVIQN
                     GTTLFQSTNDSFRVQVPEGGVIHDVNNTGSMLLEEVTQGYGILAQLCPQEQQQIFSNV
                     CGSIFNSSSARFEEDIIHIIRYPDLDARLQFALGVSANNITTDNILSYHVQKLQEVGY
                     RNIEIVNSTETTVNVTNAQTNQTIAIVPAKAVEMTYVTNFAPNENRAGYFILTATNAT
                     APIPEMTKGYTIFYEGNSIATNATSVIQTTTTASGYLSPPLPAPVRQVFDSFELIAAE
                     EVAQTEVGEIESDCESEFETEEEEVGEEEVAQTEVGEIESDCESEFETEEEVGEGGGE
                     EEVGEGGGEEEVGEGGEVEEPANLLTVDIISNGTEGVAPATFEFEANITGGAEPYSIS
                     WNFNDSGEESEEQTVLHTFDEAGTYNVTLTITDSEGQTASASIEITIKEPPPAEETVC
                     DSSYPNLCISPPPPDLDCSNDGVPENFQVLPPDPHGFDSDNDGIGCESSSNQPEPE"
     gene            3419..3733
                     /gene="rpl12p"
                     /locus_tag="VE308_04850"
     CDS             3419..3733
                     /gene="rpl12p"
                     /locus_tag="VE308_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HZB00330.1"
                     /translation="MEYVYAALLLHKLKQNITEDSVKNVVQAAGVTPDDVRVKALVAA
                     LSEVQIDEALKAAPVAVAAAAAAAAPAGTAAPAAGGEEAPAKEEEKKEEEALEGLSSL
                     FG"
     gene            complement(3938..>4519)
                     /locus_tag="VE308_04855"
     CDS             complement(3938..>4519)
                     /locus_tag="VE308_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZB00331.1"
                     /translation="LSLIFAQNKVFLPAKGGDIATKEIFIPSGNTGITPGPVLSEFKT
                     ANVPTKIDQGTIWVSKDTLVAKPRDVISTPLASLLSKLNVKPIEAGISVSFAIAEGLQ
                     FKEEDLRINLDEYKQELVRSFEQALALATEAGYMTPETVKPLLVKAQQQARSLAAEAA
                     YLTSETAGFVLPRAQMQAQAVANKAKERGYSPQ"
BASE COUNT         1188 a          976 c          964 g         1391 t
ORIGIN      
        1 aataataata ataataataa tatattgcag tgggccgtaa gggatttgaa ccctcgacct
       61 ttcgattatc agtcgaacgc tcctccaagc tgagctaacg gcccggtcat ttcaaaattg
      121 ctagcgttat ttaattgtgt atcaacaccg gtacactttt aacccgaata cttcccccta
      181 atttagatct aaaataggca agaagaaggt actttctgaa tcagagctaa aggaaatggt
      241 catgccccaa gagggcgagt tgctgggccg tgtgatcaag ttagtcggcg gcgacaacat
      301 tatcgtaaaa tgtactgacg gtaaggtaag aacatgtagg atccgtggca agataaaacg
      361 cagaatgtgg ataagggacg cggacttagt gttggttgca ccatgggact tccaatccca
      421 aagggccgac atcatatggc gatatatttc tgcccatgca gaaaagatga aggctgacgg
      481 cctgctacaa ggtcttgact gatttttcaa ccatgaatca aatatccata tatagaaccg
      541 cagattactt attgttgttg ccgtttggta aggatagcaa agaggcttgt acgttacaat
      601 atattggaag agaactccac atacagcagc cgacgaatgc aatattcttg tgaaaagtgc
      661 gcaaaaaagt ttggctcatg gaagcaccta cggcagcaca aggttgaaag ccattcttat
      721 tgaacactta aattatcaaa atcttggcta gtttagtgtg ggccggtagt ttagtccggt
      781 agaatacctg ccttgcatgc cgattacggt agaggacacg tagggggtcg tgggttcaaa
      841 tcccacccgg tccatcttta tcactcgggc taactgcgac atcaaaatag gcttgttttt
      901 aataattgtc gcttatgcta tagattctat cgtcttaatg ctaacgttga tgtttcacca
      961 tcagcagcag cagcagcatt tgacctgtct atgtgtcatc gtcttctggt gttgacccat
     1021 agtcatcatc atggcttgtt ccgacaacag ttattatcca tgataatcat ttggttattc
     1081 tggctcaggc tgattgcttg aactctcaca tcctatgccg tcattatccg aatcaaaccc
     1141 gtgtggatct ggtggcagta cctgaaagtt ttcaggaaca ccatcgttgc tgcagtctag
     1201 atctggtggc ggaggtgata tacacagatt tgggtatgag gaatcgcaga cagtttcttc
     1261 tgctggtggg ggttctttga tggtgatttc tatacttgca gatgcagttt gaccctcgct
     1321 gtctgtgata gtaagagtaa cattataggt tccagcttca tcaaatgtat gtaaaactgt
     1381 ctgctcctca ctttcttctc cgctgtcgtt aaaattccag ctaatggaat agggttctgc
     1441 gccgcctgtt atattggctt caaattcaaa ggtggccggt gcaactcctt ctgtgccatt
     1501 tgaaataata tcaactgtta gaaggttggc aggttcctct acttctcctc cttctcctac
     1561 ttcttcttct cctcctcctt ctcctacttc ttcttctcct cctccttctc ctacttcttc
     1621 ttctgtttca aattcacttt cacaatcact ttcaatctct cctacttctg tctgtgctac
     1681 ttcttcttct cctacttctt cttcttctgt ttcaaattca ctttcacaat cactttcaat
     1741 ctctcctact tctgtctgtg ctacttcttc cgctgctatt agctcaaatg aatcaaatac
     1801 ctgcctgact ggtgctggta atggtggtga taaatagcca gatgctgttg ttgttgtttg
     1861 tattactgag gtagcattag tagcaataga attgccttca taaaagatag tatacccctt
     1921 tgtcatctcc gggattggag ctgttgcatt tgtagcagtt aaaatgaaat aacctgctct
     1981 attttcattg ggcgcaaaat tagtcacata tgtcatttct acagctttgg ctggcactat
     2041 tgctattgtt tggtttgtct gcgcgtttgt aacatttact gtcgtctctg tactgtttac
     2101 aatctctatg ttcctgtaac caacttcctg aagcttttgt acgtgatatg atagaatatt
     2161 gtcagttgtg atgttgttgg cagatactcc aagagcaaat tggagtctag cgtccaagtc
     2221 aggatatcgt ataatgtgta tgatgtcttc ttcaaatcgc gcagagctgc tattgaatat
     2281 gcttccgcaa acattggaaa atatttgttg ttgctcttgt ggacaaagtt gtgctagtat
     2341 tccatagcct tgcgttacct cttctaacaa catagagcct gtattattaa catcgtgaat
     2401 taccccgccc tccggtacct gtactctgaa gctgtcattt gtgctttgaa ataatgttgt
     2461 tccattctga atcactggtg gttggttggt cggtagtagc tgttgttgcg cgtcaattat
     2521 cgtgaaattg gcaacagcta aaataatgac tgcaaatgct gctattatct ccatcggtgt
     2581 tattagagaa accaataatg gtgaatacat ctgtataatg cccaaaatta gacatgttct
     2641 tgaagctaaa taagaggttg gattgattat ctcctaaaaa ttatgtaacg tgagacaaca
     2701 gatggttttc ataataagtt ctctcgcaaa agtacatagg atagttatag ttgtttctgc
     2761 atcttactct attattttca ataaattaat tcctgctaga tctccttatt tgactggtcc
     2821 agccctaaaa accctaagtc ctgcacccca agatgggcat gaaataataa cctgcaaaga
     2881 catcgtattt cagcacttca taaaagaagc actcaaaact gtgtatggag tctctccatg
     2941 agaccatttc attggctagc ttttcaaaac tacttcgctg gatgttacag gggctatgag
     3001 acatgaaaag aaaaaggtgg acagggtggg atttgagcag ttaaggttct ctttccatag
     3061 cagctgctgc tgccacttta ggtaggcggt agagggggat gtttttacat agtgatgagg
     3121 tcagggttca gatcgcaccc ggtccactat ataatatttg ctcacgttga ctaatgtcat
     3181 gactttcaca aaaccggcta taccagctat atgatgttgc atatcgttat tgtgataaag
     3241 taaaagcttc ggtcagccgg tttctcaaaa aataagtcaa tatagcaaaa atagtttcac
     3301 ttcaatatgc agtgcagatt agatggcata aacatactgt aggctataca ttatcaaact
     3361 gacaaatcct gccagggagc ggtaggttca aatatggttc tgagtcgatg ctttctccat
     3421 ggaatacgta tatgccgctt tactgctaca caaattgaag cagaatatca ctgaggatag
     3481 cgttaaaaat gttgtccagg ccgcaggcgt gaccccagat gatgtgaggg tgaaggctct
     3541 ggtagcggca ttaagcgaag tacaaattga tgaggccttg aaggctgcgc cagttgctgt
     3601 cgcagcagcc gcagccgcag ccgctccagc aggtacagcc gctccagcag ctggtggaga
     3661 agaagcccca gccaaagagg aagaaaagaa agaagaagaa gcgctcgaag gtctctcgtc
     3721 gctctttggt tagatttctt ttaagattct ctaacttcca ttttttcttt ttttgctaac
     3781 acagctagcc ctttgaaata ccaaaattat ggaaaagcaa accgacaacg tcgaagaacg
     3841 tggatcccta ctattcagta gcggtataga gcgatcacag tcaaacagac acacacacat
     3901 gtatcctctc aattcttata tttttaattg aagaaaatta ttgtgggctg tagccccttt
     3961 cctttgcctt gtttgctaca gcctgagctt gcatttgagc tcttggcagc acaaagcctg
     4021 cagtctcaga tgtgaggtat gctgcttctg ctgcaagtga tcttgcctgc tgctgtgcct
     4081 tgactaagag cggtttgacc gtttccggtg tcatgtagcc ggcctctgtt gctaaagcaa
     4141 gagcctgctc aaatgatctc acaagttctt gcttatattc atcaaggttg atcctaagat
     4201 cctcctcctt gaactgcagc ccttctgcga tcgcaaagct tactgaaatg ccggcttcaa
     4261 tcggcttgac atttagcttg ctcaaaagcg acgcaagggg cgtcgaaatt acatctctag
     4321 gctttgcaac taaagtatcc ttgcttaccc atatcgttcc ctgatctatc ttagtcggga
     4381 catttgccgt cttgaattct gacagaacgg gtccaggcgt aatccctgtg ttgcctgatg
     4441 gaatgaaaat ttcctttgtg gcaatgtcgc cgcccttggc aggaaggaag actttgttct
     4501 gtgcgaatat caagctcag
//
LOCUS       DASQTF010000203         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_348150,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000203 DASQTF010000000
VERSION     DASQTF010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348150"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1339)
                     /gene="fen"
                     /locus_tag="VE308_04860"
     CDS             complement(314..1339)
                     /gene="fen"
                     /locus_tag="VE308_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HZB00332.1"
                     /translation="MGLDLKPLVTPRPLKLSELAGKVIAIDAYNTIYQFLSIIRGPTG
                     ELLTNSKGEVTSHLSGLFYRNANLLMDNIKLVYVFDGKANELKIGEIERRSQLKKEAT
                     EKYQLAIEQGRMEDARKYSTRTAVLTQEMVEDSKKLLSYLGIPYIQAPSEGESAASYL
                     TRHDMAFAVGSQDYDSILFGAKKLVRNLAISGKRKVPNRNTYIDIEPEMLEHDKVLHE
                     TDLTHEQLVDVGILIGTDFNPGGFTGIGPKTAIKLIKENGRLEDVKKIKSLLPQVPYQ
                     EIRKIFLDPEEPKVDKIEFTAVNRENVLDFLCVKKSFSTDRVSSTLDKLQKSIENRNQ
                     SLEQWFG"
     gene            complement(1615..2337)
                     /locus_tag="VE308_04865"
     CDS             complement(1615..2337)
                     /locus_tag="VE308_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZB00333.1"
                     /translation="MPEEKLPVPTREEHIIKPQIERLTAIKPEPLFESIVGYSDVKRL
                     FQLSLSSEKPVHLLLVGPPASAKTLFMVECMRLERSYFTLGSHSTKSGMIDYLFDKRP
                     RYLIVDEIEHMPTKDQTALLSLMETGILAETKFQKTRNTHLKTWVFATSNATDRMLTP
                     LLSRFVVLHFKQYSFGSFQEVCTHILGREGVAPDVAAEIAEAVWTKLKSKDIRDCIKI
                     GRLAKTKEDVKWIAQTLRAYKR"
     gene            complement(2417..2734)
                     /locus_tag="VE308_04870"
     CDS             complement(2417..2734)
                     /locus_tag="VE308_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00334.1"
                     /translation="MTLEELRQKAFFQNTIDVWIAYSEERGWDWYNIDAYRCFIGHLT
                     SKGIKIQKFPLCVKESGGTYERGKDKAQFLDQLSKISSADAAAYTVKLTGETIGVIRL
                     FVN"
BASE COUNT          728 a          648 c          600 g          821 t
ORIGIN      
        1 ctgcaatttc tgcgaaccta ccactatctc taatcttgaa ccttaatgaa gtgtggtaac
       61 tattcctcat agcagagaga ggcgcttgga caattcacgc aaatgggggt gtgaggaaat
      121 cacataaggg gcaaagatcc agctgatatg gaattacaaa agacgcccaa gatacaaaag
      181 agagagtgcc aagtatatag tttggaagct gttcgataga agaccgtcag gacacttaat
      241 ttgtcaagtt ttgtcagaga gacgcactca ttataacaag gtctcaatta tttgactcta
      301 ttatcacact ctgttagcca aaccactgtt caagcgactg gttgcggttt tctatggatt
      361 tttgaagctt gtcaagcgtg ctgctcaccc tatcagttga aaagcttttc ttgacacaaa
      421 gaaaatccag tacattttca cgattgaccg cggtaaattc aatcttgtcg acctttggtt
      481 cttcaggatc gagaaagatc ttgcgtatct cttgataggg gacttgtgga agcaagctct
      541 tgattttttt tacatcttcc agcctgccat tttccttaat taatttgatt gcagtcttcg
      601 ggccaatacc agtaaagccg ccagggttga aatcggtgcc gataagtatg ccaacatcca
      661 ctagctgctc gtgtgttagg tcagtctcat gcaacacctt gtcatgctca agcatttcag
      721 gctcgatatc gatgtaagtg ttgcgatttg gcaccttgcg cttgccgcta attgccagat
      781 tgcgcaccag cttcttggcc ccaaacaata tcgagtcgta atcttggctt ccaactgcaa
      841 aggccatatc atgccttgta agatatgagg ctgccgattc gccctcagag ggcgcctgaa
      901 tatagggaat gccaagatag gaaagcaatt tttttgaatc ttccaccatc tcctgagtca
      961 ggactgccgt gcgtgtacta tacttacgtg cgtcctccat cctaccctgc tcaattgcaa
     1021 gttgatactt ttctgtcgct tcttttttca gctggcttct gcgctcaatt tcacctatct
     1081 tgagctcgtt tgccttgccg tcaaaaacgt aaacgagctt gatattatcc atcagtaggt
     1141 tggcattcct atagaatagg ccgctcaggt ggcttgttac ttcccccttg ctgttcgtaa
     1201 gcagttcccc agtcggtccc cttataatac tgaggaactg ataaatcgta ttgtaagcat
     1261 caattgcaat aaccttgcct gccagctctg acaatttgag cggtctagga gtcaccagtg
     1321 gcttgagatc taagcccatt tcttgttatc acacaataca cagaatggca ccacaaaaac
     1381 tttaactctg tattaccaaa attcttgcgc caagaaacta gacaggtatc gccattataa
     1441 ttattccgaa aaacaaaaaa aggatcaatg aaatttaatg actttttacc acatatcgaa
     1501 aagtatgggc ttttttcttg caaagacaaa aaaagagaga tatctgagaa ttgctagcca
     1561 gcacatatca agctgcttta gcatgtcacg atgagctcaa caacagcaaa tgagctaccg
     1621 cttataggct ctcagcgttt gggctatcca tttaacgtct tcttttgtct ttgccaagcg
     1681 cccgatcttt atacagtcac ggatgtcctt tgacttgagt tttgtccata ccgcctccgc
     1741 tatttctgca gcgacatcag gtgcgacccc ttcgcggcca aggatatgag tgcatacttc
     1801 ttgaaatgaa ccaaatgagt attgtttgaa gtggagcact acaaaccttg ataaaagcgg
     1861 tgtcagcatt ctgtcagtgg cattgctagt cgcaaagacc catgtcttta ggtgggtgtt
     1921 acgcgtcttt tggaacttgg tttccgctag gatgcctgtc tccatcaggc tcagtagtgc
     1981 agtctgatcc ttcgtgggca tatgttctat ctcatccaca attagatatc gcggcctttt
     2041 atcaaacaga taatctatca tgccggattt agtgctgtgg cttccaagtg tgaagtagga
     2101 gcgttcgagt ctcatgcatt caaccatgaa aagggtcttg gcagatgctg gtgggccaac
     2161 aagtaacaag tgaacaggtt tttcagaaga tagcgatagc tgaaacagtc tttttacatc
     2221 actgtagcct actatgctct caaatagcgg ctccggttta atggcagtca gcctttcaat
     2281 ttgaggcttg attatgtgct cttcgcgcgt tggaactggc agtttctctt caggcacttc
     2341 acattatata gatagacaat agattttaga ggttcctact aaactgtaat atctacgagc
     2401 tgaaattttc tagactctag ttgacgaaca ggcgtatgac acctatcgtt tcgcccgtca
     2461 atttgacggt gtaggccgcc gcatcggcag aggatatctt tgagagctgg tcaaggaatt
     2521 gagccttgtc cttaccgcgc tcatatgtac ctccggattc cttgacgcag agggggaact
     2581 tttggatctt gatacctttt gaggtaaggt gtccgataaa gcatcgatat gcatcaatat
     2641 tgtaccaatc ccagccgcgt tcctcgctgt acgctatcca cacatctatg gtattttgaa
     2701 aaaacgcctt ctggcgcagc tcttcaagag tcatgtgtta aaagtctgcc ctgcgcattt
     2761 tgcttccgca gcgaggacat gctccaccct tgaaccg
//
LOCUS       DASQTF010000204         6211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_451583,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000204 DASQTF010000000
VERSION     DASQTF010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451583"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VE308_04875"
     CDS             complement(<1..401)
                     /locus_tag="VE308_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZB00335.1"
                     /translation="MVESINASKEISAPLDRVWNIISNVDDDEPRYWQDLNAVFYNTS
                     KKGNIVEREVTGGLRNLKGRQTLVLYPKKAIELTLTEGRMIGTRIITPIPSRSDNEAI
                     TTRVNVSWDIKLSDNFPLLFRGVVRERIAEAT"
     gene            complement(414..785)
                     /locus_tag="VE308_04880"
     CDS             complement(414..785)
                     /locus_tag="VE308_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00336.1"
                     /translation="MSNLKLLLAVGLAAASLALVLTVVVGGPAVLQRAGIAMAFVISA
                     IALATAAFVASWKQRSFLVAGLLVASGIILMIPGLIAAGYLAVIVFPGPILGVIFGLV
                     IFGLGVAKGIRSAKTAVITAR"
     gene            1112..2245
                     /locus_tag="VE308_04885"
     CDS             1112..2245
                     /locus_tag="VE308_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00337.1"
                     /translation="MSGKDELIFSKEALLRTHAGKDIIKQRMLQSKGYKQFNHYKEKT
                     EQEFPSFVQRFTLSLHKAVTSDSNPSSTIHKFTEEVNSQELDLSHSSVPDIKMRLSNP
                     DILADRVRRILDSNFVKMTFPVFSALYDGASKYFGDSVSEEKRKAVIDGHIIAIDLSE
                     PMDRIVDKDEDLGYLDDYKFMNPYILAIARSNIAQGGDAVLKAFEEGFRNARIGQHID
                     VKLKMEPASINDENMTECYKKYRAVMGTAGRNMALNRRPLSDIFHLGMAKAGECVGCG
                     NEIEDAIRNNEVKIPSWPLYFAVNMSNVQRGFELTMAKSDLYLDEAKIALDMLPENFQ
                     VKPFLEFLFLTVRHYNQYWYNELIRRAPFGEFYKKIEEAVARR"
     gene            2248..3492
                     /locus_tag="VE308_04890"
     CDS             2248..3492
                     /locus_tag="VE308_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZB00338.1"
                     /translation="MMMWSEKHRPKTVQEMVGNEDARLVAIKWLAGWVSGSRPLLLVG
                     PPGTGKTTLVHTLARQLDYDVVEMNASDIRNKEVLQARITPVFQNTANLTGKNIMLFL
                     DEVDGISGREDTGGLDTLINLMKEPTVPVIMAANKKSLKIMKLAKACKIVEFSPVPPR
                     LLLLLLDYVLQSEGVKLGSGDKTSIIDNSRGDIRSLINSAQSRAAGYSTVSNQDIVDV
                     DIADAITGYFNADTMNQAMQFIIMADASYPDPRYRLISPEERRKDMLASLFSSIVSSE
                     MEQDDLALLLNVISKADMIVGRANASRQWHLLKYVNSLIATGLYEKSRQKGIKYRQYS
                     MPWPVMGPIFARSQSTRKILDELAPAFHMSRSSAGLFVLPYFIRAMIDGKIDPVEFAL
                     DNFQDESVGQSIAKEIERVKRK"
     gene            3489..3878
                     /locus_tag="VE308_04895"
     CDS             3489..3878
                     /locus_tag="VE308_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00339.1"
                     /translation="MNDWIRLLVKYKGKCSACGKEISAGQYALWSKSSKAIKHSECKV
                     PPPPTTTEKRDPDTNRQQSTVAEVDCFICGTPVAGNTSGFEGDRYGRKAISQASVCNR
                     CLKDKNAYQNYQHAFLEKVYRVAKVKI"
     gene            complement(4057..>6211)
                     /locus_tag="VE308_04900"
     CDS             complement(4057..>6211)
                     /locus_tag="VE308_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZB00340.1"
                     /translation="VDGRKYYFCSTQCLNEFTEPERELRKLKKHVAISIALTIPIVIL
                     TLPHMFPSQLEDLIPLPMETSNYVMLALATPIQFWIGWRFYKGMWDGIKARASNMDTL
                     IALGTSAAYVYSAAVTIAPGYFPFASVYFETAAIIITLILVGRLLEIRTKEKASSAVR
                     KLMDLQPRMAKVIREGNREEEEIPVERVREGDLLVIRPGERVPVDAIVIDGVSSIDES
                     AVTGESIPVDKSRGSEVIGATINQSGLLTVKATKVGQDTVLSQIITLVENARMGKAPM
                     QRMVDQVAKYFVPVVIAVAVCAGLGWYFAGGAGLTYSVLAFVSVIIIACPCALGIATP
                     AALMMGAGKGAENGILFKSGEYLEIARKINTVVFDKTGTLTEGKPSVTDIVDMSGGIG
                     ENELLRLAAIAESGSEHPLGQSVVRKAKESGLPVSNPESFEAVSGHGLRATYAGHTIL
                     IGNRKLMHENEIEVDSRVDSILSRLEHEGKTATLVSIDSRLSGIIAMADTIKESAKEA
                     VDSLKNKMGIEVIMLTGDNEKTAKAIASRLGIDRVIAQVLPQQKEEVIAKLRSEEGRV
                     VAMVGDGINDAPALARADLGIAIGSGTDVAKETGGIILIKDDVRDVAIALELGKKTVS
                     KIKQNLFWAFAYNTGLIPIAGGALVPFLGLSVFGWLPILAGAAMALSSVTVVGNSILL
                     GRYRPRYAATKPKEEQVYSNRELKSVYTPSEAHAT"
BASE COUNT         1694 a         1456 c         1353 g         1708 t
ORIGIN      
        1 gttgcttctg caatacgttc tcttactacg cctctaaaca atagaggaaa gttgtcagac
       61 aacttgatat cccaagatac attgactctt gttgttattg cttcgttatc gctcctagat
      121 gggattggtg taataatcct agtacctatc atccgacctt cagtaagcgt tagttctatc
      181 gctttcttag gatacagcac cagggtctgg cgacctttta agtttctaag gcctcctgta
      241 acctctcttt caactatgtt gcctttcttg ctggtgttgt agaagacggc attcagatcc
      301 tgccaatatc gtggttcatc atcgtccacg ttggagatga tgttccatac tcggtcaagg
      361 ggtgccgaga tctctttgct ggcgttaata ctctcaacca ttttcgtgta tcactacctg
      421 gccgttatca cggctgtctt tgcagacctt atgcccttcg ccactcctag cccaaatatc
      481 acaagcccga agatgacgcc taggatagga cccgggaaca caatgactgc tagataccct
      541 gcggctatca ggccgggtat catgaggatg atgccgctag caaccagcaa tcctgctact
      601 agaaatgacc tctgcttcca tgacgcaaca aatgctgctg tcgctagcgc aatcgcgctt
      661 atgacgaacg ccattgcgat tcctgctcgc tgcagtaccg ctggaccacc tacaacaact
      721 gtcagaacca aagcaagtga tgccgcggcc agacccactg ccaatagtag ttttaaattt
      781 gacatgggct gacttctgca tgaattactt taaccattgt tcaccattga cacgtaacat
      841 agttacatcc gtgtttatct aggtctgcca taaaggcatt tttaggaatg cgcataataa
      901 atcatattac aagtactcga agttgctatt gtgaaatcta gtttaatcct gacatgcttt
      961 tttcgcaaag aatagaattg ataattgctg cacttgattg aaacacaggt tcgctgagcg
     1021 gaagacacca aaaaggacgc aagaaagttt ctgaagctaa aacattagat tatgaatgat
     1081 taaataacaa taggtggcag aagccagaga aatgagtggc aaggatgaat tgattttttc
     1141 aaaggaggct ctgctgagga cgcatgccgg taaggacata atcaagcaga gaatgctgca
     1201 aagtaagggc tacaagcagt tcaaccacta caaggaaaag acagagcagg aatttcctag
     1261 tttcgtacag cggttcacct tgtccttgca taaagcagta acttccgaca gtaatccctc
     1321 cagtacaata cacaaattca ctgaagaggt caattcacaa gagcttgacc tctcacacag
     1381 tagcgtgcca gacatcaaaa tgagattatc aaatcctgac attcttgcag acagagtcag
     1441 gcgcatacta gattctaatt ttgtcaagat gacatttccg gttttcagcg ccctttatga
     1501 tggggcatct aaatattttg gcgacagtgt ttctgaagaa aagagaaaag ctgttatcga
     1561 tggccacata atcgcgattg acctttcaga gcctatggac aggatagttg acaaggacga
     1621 agatctgggg tatcttgacg attacaagtt catgaaccca tacatacttg caatagcgcg
     1681 cagcaatatc gctcaaggag gcgatgcagt tttaaaggct tttgaagagg gctttcgtaa
     1741 tgccagaatt ggccagcata tagatgtcaa gctaaagatg gaaccggcat caattaatga
     1801 tgaaaacatg actgaatgct ataagaaata tcgggcagtg atggggactg ctggcaggaa
     1861 tatggcgctc aatcgccgcc cactttctga cattttccat cttggcatgg ccaaggctgg
     1921 tgagtgtgtc ggctgtggca atgagatcga agatgccata aggaacaacg aagtcaagat
     1981 tccttcatgg cccctctact ttgctgtcaa catgagtaat gtccagcgtg gctttgaact
     2041 cacaatggca aagagcgatc tctatctcga tgaagcaaaa attgcgcttg acatgctgcc
     2101 tgagaacttt caggtcaagc cttttttgga atttcttttc ttaactgtcc gtcactacaa
     2161 tcagtactgg tataatgagc ttatacgccg tgcaccattt ggtgaattct ataaaaagat
     2221 agaagaagct gttgcaagaa gatgataatg atgatgtggt ctgaaaagca caggccaaag
     2281 actgtgcaag aaatggtggg taacgaggat gcccgccttg ttgcaataaa gtggttagcc
     2341 ggctgggtga gtggcagtag accgcttcta ttagtgggtc caccgggcac gggcaagacc
     2401 accctagtac acacacttgc aaggcaactt gattatgatg tagtagaaat gaatgcaagc
     2461 gacatacgca acaaggaagt cctgcaagca agaataacac cagtttttca aaataccgcc
     2521 aacctgactg gaaaaaacat aatgctcttc ttggatgagg tagacggcat ctctggcagg
     2581 gaggacacag gaggtcttga cactcttatc aatctaatga aagagccaac cgtgccagtg
     2641 ataatggctg caaacaaaaa gtcacttaag ataatgaagc ttgccaaggc ttgcaaaata
     2701 gtggagttta gtccagtgcc cccacggcta ttgttgcttc ttctagatta tgtactgcag
     2761 agcgaaggag taaaacttgg atcaggcgac aagacatcaa taatagacaa cagtagagga
     2821 gacatccgat cgttgataaa cagcgcacag tcacgtgccg ccggctattc tactgtttca
     2881 aaccaagata tcgtagatgt ggacattgca gatgcaatca ctggttactt taatgccgac
     2941 actatgaacc aggcgatgca gttcataata atggcggatg cttcatatcc tgacccaaga
     3001 tatcgattga tttcaccaga ggaaagacgg aaagatatgc tagcatcctt attctctagc
     3061 atagtttcct cagaaatgga acaggatgat cttgctttgt tgctgaatgt aatttcaaaa
     3121 gcagatatga tcgttggaag ggctaacgca tcaaggcaat ggcaccttct aaaatacgta
     3181 aatagcctga tcgcaacagg gttatacgaa aaatcccgcc agaagggcat caaataccgc
     3241 cagtattcaa tgccgtggcc tgtgatggga ccgatatttg cccgctcgca gtcgacaagg
     3301 aagattttgg atgaacttgc tccggctttt catatgtcaa gaagctctgc aggattattt
     3361 gtcctgccat attttatcag ggctatgatt gatggaaaaa tagacccagt cgagtttgca
     3421 ctagataact ttcaggacga gtcagttggc cagtcgatag caaaagagat cgagagagtg
     3481 aaaagaaaat gaacgattgg attagacttt tggtgaaata caagggcaag tgctctgcat
     3541 gcggcaagga aatatctgca ggccaatatg ccctttggtc aaagagtagc aaggcgatca
     3601 agcatagcga atgcaaggta cctccgccgc caacaacaac tgaaaaaaga gaccctgata
     3661 cgaatagaca gcagtcgacc gtagcagagg ttgattgctt tatctgcggc acgcctgtcg
     3721 caggcaacac ttccgggttt gagggcgacc gctatggcag aaaggcaata tcgcaggcgt
     3781 ctgtctgcaa tagatgtctg aaagacaaga atgcctacca gaactaccag catgcattcc
     3841 ttgaaaaggt ttacagagta gcaaaagtta aaatctgatg ttgtttcgca gcaataagat
     3901 atttgcattg gtgattatcg tcacaattaa gtattcgtct cttgcgtggg atctatcgct
     3961 taatcatcct gtgtgttata cttattccga cgtttcacta tgtggcaagt tggttattat
     4021 tagtactagt ccgaacagta ccttttttag aagccattaa gtagcatgag cctcactcgg
     4081 cgtatagaca cttttcagtt ctctgttaga gtaaacctgc tcttcctttg gttttgtcgc
     4141 tgcatacctt ggtctgtatc ttccaagcag tatggagttt cctactactg ttaccgagct
     4201 cagagccata gctgctccag caagtatagg cagccatccg aacacactta gtccaaggaa
     4261 gggaaccaaa gctcctcctg ctatgggtat caagccggtg ttgtatgcaa acgcccaaaa
     4321 caggttctgc tttattttcg atacagtctt tttgcccaat tccagagcaa tcgctacgtc
     4381 tctcacatcg tcttttatca gaattatccc acccgtttcc tttgctacat cagttccaga
     4441 gcctatcgct atccccaaat ctgcacgtgc taacgctggt gcatcattta tgccgtctcc
     4501 caccatcgcc acgactctgc cctcttctga tctgagtttt gcaatcacct cttctttctg
     4561 ctgcggtaat acttgagcaa ttactctatc aatgccaagc ctggaagcaa tagccttggc
     4621 agttttctcg ttgtcgcctg taagcattat cacttctata cccattttgt tcttgagcga
     4681 gtcgaccgcc tcctttgcac tttccttgat tgtatctgcc attgcgatta ttccagagag
     4741 tctactgtca attgatacaa gtgtagcagt cttgccttca tgttctagcc ttgatagtat
     4801 tgaatcgacg cgactgtcaa cctcgatctc gttttcatgc atcagctttc tgttcccaat
     4861 taggattgtg tgacctgcat atgtcgccct gagcccgtga cctgacactg cttcaaatga
     4921 ctcagggttt gatacaggca gaccagactc cttcgccttt ctgactactg attgtcctag
     4981 tggatgctca gaacccgatt ctgcaattgc tgcaagcctc aatagctcat tttcgccgat
     5041 gcctcctgac atatctacta tatctgtgac agacggcttg ccttctgtca gggtgcctgt
     5101 cttgtcaaat actacagtgt ttatctttct tgcaatttcc agatattcgc cgcttttgaa
     5161 tagtatgccg ttttccgcac ccttgccagc ccccatcatc aatgccgcag gagtggcgat
     5221 gccaagtgca caggggcatg ctatgattat cactgatacg aacgcaagga ctgagtatgt
     5281 tagccctgct cctcctgcaa aataccatcc aagcccagcg caaactgcca ctgcaattac
     5341 tactggtacg aagtactttg caacttggtc taccatcctc tgcataggtg ccttacccat
     5401 ccttgcgttc tctacaagtg tgattatctg ggagagcacg gtatcttgac ccactttagt
     5461 agctttgact gtaagaagtc cgctctggtt aattgtagcg ccgataactt cgcttcccct
     5521 tgacttgtct actgggatgc tctcgccagt gactgccgac tcatctattg aagatactcc
     5581 atcaattaca atggcgtcaa caggcacacg ctcacccggt cttatgacaa gcagatctcc
     5641 ttcccgaact cgctcgacag gaatttcttc ttcctctctg tttccttccc ttatcacctt
     5701 agccatccga ggctggagat ccatcagttt ccttactgca cttgatgcct tttcctttgt
     5761 ccttatctct agcaacctgc ccaccaagat taacgttatt atgattgctg cagtttcaaa
     5821 atacactgat gcaaacggaa agtacccagg tgcaattgta acagctgcgc tgtaaacgta
     5881 tgctgcacta gtccctagcg ctattagcgt atccatgtta gaggctctag cctttattcc
     5941 atcccacata cccttgtaga agcgccagcc tatccagaac tggattggtg ttgctagcgc
     6001 tagcataaca tagttgctag tctccattgg aagaggaatt aagtcttcaa gctgcgaagg
     6061 aaacatgtgt ggaagagtta gtataacaat aggaattgta agggcaatgc ttattgcaac
     6121 atgctttttc agctttcgta attctctttc tggctctgta aattcattaa ggcattgtgt
     6181 gctgcagaaa tagtatttcc ttccatccac t
//
LOCUS       DASQTF010000205         3745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_998023,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000205 DASQTF010000000
VERSION     DASQTF010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998023"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(452..1099)
                     /gene="cofC"
                     /locus_tag="VE308_04905"
     CDS             complement(452..1099)
                     /gene="cofC"
                     /locus_tag="VE308_04905"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HZB00341.1"
                     /translation="MMKTFAIVPVKRFENAKSRLSSMLDIDDRIRLSSLMLEDTLQIL
                     SVADSLKQVIIVSADRRAEAIATKHGANFLLEEKERGVNSAVVLADIYCMKQTADATM
                     VIPQDLPLLGAIDISKACELAENESRCIVICPSLRYDGTNMLLRKPPSVIATFYDTDS
                     YNMHVKAAIKIGVPVKRFFSKTIMYDVDNPEDALQLIKEEAVTATRTIEFIKSKF"
     gene            complement(1104..2033)
                     /gene="cofD"
                     /locus_tag="VE308_04910"
     CDS             complement(1104..2033)
                     /gene="cofD"
                     /locus_tag="VE308_04910"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HZB00342.1"
                     /translation="MTITVLAGGTGSIKLVRGLATIERDIVVICNVGDNIWIYGLYVC
                     PDIDTIIYGLANILDQKRGWGIKGDSFQCLAQMKKIGAPSWFGLGDKDLATHLLRTSM
                     MKDGKSLSEITGFFRKCYSIRAQLIPATDREVTTSILTTSRGDMHLQEFWVKYKGRPR
                     VSGIRYDNASSSTANPAAIDAIKRCQAVVIAPANPVSSIGPILALSDLRKELVQNRDK
                     VLAISPLIGRKALSGPAVKYMRALGLESSSVGVAKHYRDFVSKFIISRKDHRMKLQIE
                     ALGMKVHETNITMKARRDEVRLGMHLLKMVEKN"
     gene            2586..3557
                     /locus_tag="VE308_04915"
     CDS             2586..3557
                     /locus_tag="VE308_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00343.1"
                     /translation="MAAVLDPGSDTSPASYVGVRTLTLEYPVGSSLAQALDGQNQRIE
                     FSLNGTATGQDDTGISGVIAALNRALVQADSPVQASQVEVSYTGVLRGGPTSTLISYR
                     IDLQPTLQSYVLQGGESGQGSQVVDLEWRGITINEPLVVNAPDVGQININYPMGLLQV
                     LYPSIAQKFESTQAREVLDESILNFGEFNAPLSSWHVLFDPVGAYGGSVGLNGTGGAK
                     ALSVYSLGESSLREGAHGIEEKTATATIDGANVNVQSTTPPPSGQIQLAGYSKAQESG
                     GAWHAVVTTEAPEGVQTSTGGFPIQVLLVLGGMMGAIAIFILFKARK"
BASE COUNT         1009 a          814 c          826 g         1096 t
ORIGIN      
        1 cttctcgggt tccatggctg caagtaccct tattagtaga tcagcatgca tatcgagtac
       61 atactcactc gatcgttaca tcctgttata tttaagccca gcaactaata tcagatctca
      121 cgacctgtca ttttttcttc agcatagtga tctgcatcat ttcctagggc gtctcgaacg
      181 ctaactctca agtaaggaga ctggcatggt gggcgtgggg aggacgacca tttcgatttt
      241 atcaaaaaca atgacagagt ctttgagaca ctgaactgaa ggagcgttat cgtgatgtat
      301 ataaatttca taagtgtgta taagtatacc aattctaact gcgacaagtg cttagtatat
      361 ttcaagtcat cagaggtggc tttgtggata tatatatcac ttatatcaaa aagtaaagcc
      421 gtgtgagtag ccttgctaaa atcatcttaa tttagaattt tgatttaata aattctatgg
      481 tcctggtggc ggtaacagct tcttctttta tcagttgtag tgcatcttcg gggttatcaa
      541 catcgtacat gatagtcttt gaaaaaaagc gctttacagg tacaccaatt ttgatggctg
      601 ccttgacatg catgttataa ctgtcagtgt catagaaagt ggcaatcaca gaggggggct
      661 ttcttaatag catatttgta ccatcataac gaagcgaggg gcagatcact atgcacctgc
      721 tctcattttc agccagctca catgctttgg aaatatcaat tgcaccaaga agcggcagat
      781 cctgcggaat aaccattgtt gcatctgcag tttgtttcat acaatagata tcagccaaca
      841 cgactgccga gttgacccct ctctcctttt cttcgagcag gaagtttgct ccatgctttg
      901 tcgctattgc ttctgcacgc ctgtctgctg atactatgat cacttgcttc agtgagtcgg
      961 cgacggaaag gatctgcagt gtatcttcta gcattagcga tgaaaggcgt atgcggtcgt
     1021 cgatatcaag cattgacgac agtcgtgact ttgcgttttc aaaccttttt acgggaacga
     1081 tggcaaaggt cttcatcatg attttaattc ttttcgacca tttttaagag atgcatgcca
     1141 agtctaactt catcacgtct ggctttcatg gttatgttag tttcgtgtac cttcatacca
     1201 agagcctcaa tctgtaattt catgcgatga tctttcctag agattataaa cttgctaaca
     1261 aagtcacggt aatgcttagc cactccaact gaggagctct caagcccaag agccctcata
     1321 tattttactg ccggtccgct tagcgccttt ctgcctatca gaggagaaat ggctaaaact
     1381 ttgtctcggt tttgcacgag ttcttttcgc agatctgaaa gagccaggat gggccctatg
     1441 cttgaaaccg ggtttgcagg tgcaattact acggcttggc atcgcttaat agcgtctatg
     1501 gcagcaggat ttgctgtact gctgctagca ttatcatacc ttatcccgct tactctaggc
     1561 cttcccttat atttgaccca aaattcttgt aggtgcatat cgcctctgct agtagtgaga
     1621 atacttgtag tcacctctct atccgtagca gggataagtt gggctctaat agaatagcac
     1681 ttcctaaaaa aaccggtaat ttcagacaga ctcttgccgt ccttcatcat gcttgttcgg
     1741 agcaagtggg tcgcaaggtc tttatcacct aaaccaaacc aagagggggc accaattttt
     1801 ttcatctggg ccaaacactg aaatgaatca cctttgatgc cccatcctcg tttctgatca
     1861 aggatgtttg caagtccata aattatagta tcaatgtcgg ggcatacata tagaccgtat
     1921 atccatatat tgtcgccaac attgcagatt acgactatgt ctctttcaat agtggcaagt
     1981 cctctgacca gctttatgga acccgttcca cccgcaagaa ccgttatcgt caaatgatat
     2041 gttgtagcta aatgatgtgc attttattat atctgccgac atgatccttt tgtatacaaa
     2101 tcgatcagtt agatttctaa ttcaacttgt acattcggcc gattaggtag gagataggta
     2161 aaacggctac aggtaataaa taaatatatc attttttcct tgctcgagtt aaaaagaatc
     2221 gaggtagaaa atcgtatatt gttggttgat gattatatta tttttctgcg gatccatctg
     2281 ccttggtgac aatgtaacag ctatctggat ggggatatct tgtgcaaaaa aatattcaat
     2341 tacattgtaa aatcatcaat tttttgaaat gttctgaggg caatttgccc cgagcttatg
     2401 atagcaaata cttctgactt catgagacca aatgtctctt tgctactcat gaaagagaat
     2461 caggaataaa gattataaac gaggcacaca gaggaatatt tgtgaagtac ttcaccatag
     2521 tactttctgg aatactcttg gtacttttaa tctcagtttt tgctactcag gcttatgctg
     2581 cagatttggc ggcagtactt gatccgggga gcgatacctc acccgcttcc tatgtaggcg
     2641 tcaggacgct tacattggag taccctgtgg gcagctctct ggcacaggca cttgacggcc
     2701 agaatcaacg cattgagttt tcgctgaatg ggacggctac tggacaggat gacaccggga
     2761 ttagtggtgt cattgcggcg ctaaatagag ctcttgtgca agctgatagc ccagttcaag
     2821 catcgcaggt tgaagtttcc tataccggag tccttagagg aggacccact agcacgttga
     2881 tatcctacag aatagaccta caaccaacct tgcaaagtta tgtgcttcaa ggtggagaga
     2941 gtggccaagg tagtcaggta gttgacctag aatggagagg cattacgata aacgaaccac
     3001 tggtggtgaa tgcgccagat gtcggtcaaa tcaacatcaa ttaccctatg ggtctattgc
     3061 aggtactata tccatctatt gctcagaaat ttgaaagtac acaagctaga gaggtcttgg
     3121 acgaatcgat cttaaacttc ggagagttta acgctccact gagctcatgg catgttcttt
     3181 tcgatcctgt aggtgcctat ggtggcagcg taggtttgaa tggaaccgga ggagccaagg
     3241 cattgtctgt ctattctctt ggagagagca gtctaagaga aggtgcgcac ggaatcgaag
     3301 aaaagactgc aactgcaacg attgatggag caaatgtgaa tgtccagtct acgaccccac
     3361 ccccaagcgg acagatacag ttagcggggt attcaaaggc ccaagagtca ggtggcgcat
     3421 ggcatgcggt agtgacaaca gaggcccccg aaggagttca gacatcaacg ggtggattcc
     3481 ctatccaagt gcttttggtc cttggcggta tgatgggcgc aatagccatc tttattctct
     3541 tcaaggcgag aaagtaacaa caaaagctaa aatcatgctg tcaatctatt ctaaccgaca
     3601 tgtctctctc tctctctctg aggctagcct tcttccctca aatagtatcc tgcgtcagcg
     3661 atagtagaga ctgttactct gcctttctgg aaaagcgacc tgccggcaaa tcgtgttctt
     3721 ccaaccgcta ttgcctcatg ggctg
//
LOCUS       DASQTF010000206         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_824393,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000206 DASQTF010000000
VERSION     DASQTF010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824393"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1342)
                     /locus_tag="VE308_04920"
     CDS             complement(92..1342)
                     /locus_tag="VE308_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZB00344.1"
                     /translation="MSLAPQELENSASKYAAEAIRLDSQGSRGMAINSYQRAIEALVK
                     LVQIYPDYKLNKVYMERANAYQNRIKALQMSHGLEEEKMAPTQIDKIKLDPPNGHGSK
                     HSSASSSTTSTSSRSSGNVETLKADFDDLVMKEKPKVSWKEVIGLEDAKRAMRESIVY
                     PTKRADLFPLGWPRGILLYGPPGCGKTLLAAAAAAEIDGYFINVDAASMMSKWLGEAE
                     KNISKLFKMARSLTESEGVPVLLFIDEIDSLLGTRNSEVGGEVRVKNQFLTEMDGING
                     KSKESQLYVIGATNKPWSLEVGFLRRFQKRIYVTLPASPSRTNLFLQYTSPLNVDGTL
                     RVDELAKISEGYSASDIKDICQSVQLRVVNELFESGKAMEDGTDPRPINMTDFREIFR
                     IRKPSVSVDMIRAYMRWSDQFKAL"
     gene            complement(1447..1704)
                     /locus_tag="VE308_04925"
     CDS             complement(1447..1704)
                     /locus_tag="VE308_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00345.1"
                     /translation="MSISEVRDDVEQTVEAAEASLSGLHEAVFRVSTRILPDLSREFE
                     SALPVELSTLLAKCKTNTANKATVVRTAEKEEDLKGERKEH"
     gene            complement(2105..2752)
                     /locus_tag="VE308_04930"
     CDS             complement(2105..2752)
                     /locus_tag="VE308_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00346.1"
                     /translation="MNSRWVKSESVLDSNDRDNNNNQTASLPRPGGPLKPRLDAVSKK
                     IQVQIDRLEELHSMNRLKDNDIFTKLKVSINENNTQHSTLLSSELARGRQVSRALLLS
                     CVALEKLVSRLSLASDFGDLVIILSPAMAVVKSLRSSVTPYVPEMEGELGVISELLSG
                     ILVDAGQVGGFTINFETANEQAVRFIGEVSGLVDQRMEEEFSKVPELPAIPIRLP"
     gene            2912..>3337
                     /locus_tag="VE308_04935"
     CDS             2912..>3337
                     /locus_tag="VE308_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZB00347.1"
                     /translation="MMTDTNSDLPGSLEEFGLSKYEARAYLTMIGKGSLAVSEIAYYA
                     NLPRTKVYQTVKKLEKKKLAVISKQKPLVCSAIPPEEAFAEIVNLHDRRVKNMRNIVD
                     SLQKINDESQRPKGSEERRYFILDANATLEKIGSLIADSR"
BASE COUNT          842 a          806 c          698 g          991 t
ORIGIN      
        1 ttcttaatga gcgctggatg tctcaacaac tgacaaaaaa aagatgaaat gagactagag
       61 gttcgaatgt aagaagaaga aaaaattgcg attaaagcgc cttgaattgg tcgctccacc
      121 gcatgtaagc ccttatcatg tcgacgctca cgcttggctt acggattctg aatatttctc
      181 taaaatcagt catgtttatc ggtcttggat cggtgccgtc ctccattgcc ttgccgcttt
      241 caaataattc gtttactacg cgtagttgaa cagactggca aatgtccttg atgtcgcttg
      301 cgctgtagcc ctcacttatt ttggcaagtt cgtcaaccct tagcgtccca tcaacattca
      361 gtggacttgt atactgcagg aaaaggttcg tccttgatgg gctcgctggc agcgtgacat
      421 atatcctttt ttggaacctg cgcaggaacc ccacctccag gctccaaggc ttgtttgttg
      481 cccctataac atagagctgg gattctttgc tcttgccatt gataccatcc atctcggtca
      541 gaaactggtt cttgactctg acctcgcctc ctacctcact gttacgcgtg ccgaggagcg
      601 aatcgatctc atctataaac aatagaactg gtactccttc gctttcagtt agactacgag
      661 ccatcttgaa tagttttgag atattctttt cagcctcgcc tagccactta ctcatcattg
      721 atgcagcatc gacattgatg aagtagccgt caatctctgc agctgctgca gctgcaagca
      781 acgttttgcc gcatcccggc gggccataaa gtaatatacc cctgggccag ccaagcggaa
      841 acaagtctgc cctctttgtc gggtaaacga tagattcacg cattgccctc ttagcatctt
      901 caagcccaat gacttccttc cagctcacct tgggcttttc cttcataacc aagtcatcaa
      961 agtcggcctt tagtgtttcc acatttccgc tagatctgga tgaagtagaa gtagtagaag
     1021 aagacgcact ggaatgcttg cttccatgcc catttggtgg atctagttta atcttatcaa
     1081 tttgggttgg tgccatcttc tcttcttcta gaccatgcga catctgaaga gccttgatcc
     1141 tattctgata cgcattggct ctctccatgt acactttgtt tagcttgtaa tcgggatata
     1201 tctgaacgag cttgaccagc gcctctatag cccgctgata cgaattaatt gccattccac
     1261 gtgagccttg agagtccagc ctgattgcct cggctgcata cttgctagcg ctattttcta
     1321 attcctgcgg tgcgagactc ataaatatag ttcaagccag attgggttct ttcctttgaa
     1381 ttacttatgt gtaaatctat gttgcctagc caaccaaata atttgcacct ataccgtcat
     1441 gctcgctcag tgttccttac gctctccctt cagatcttct tctttttctg ccgtccttac
     1501 aacggttgcc ttatttgccg tatttgtttt gcattttgca agtaaggtag acagctctac
     1561 agggagcgcg gattcgaact ctcgactcaa atccggcaaa atccgagtcg acactctaaa
     1621 tactgcttca tgtaatcctg aaagcgaggc ctcagctgct tcgactgttt gttcgacgtc
     1681 gtctcttact tcgctgatgg acaacgctgc attacttatt attgttataa attcatcaag
     1741 atactgcaga gtagaccctt tcagagacaa cattcaggat gagcttacct ttttctacta
     1801 tctcgattac caaagccagc tcttggtaat gcttggtaat gctcggtctt ccatttacta
     1861 ttgatgataa agtcgattcg tgattcgagc cttcaacgca gaatattcat tgcataggcg
     1921 ttcttttgga cagttgttag cgggggtttg ttctctcctt tctcgaagcg ttccaattct
     1981 tggtgaacag caaatatcga tatcataaga tttatggaaa aacaacgaaa aactgggctg
     2041 tcaaaaattg ttgtgcagtt tgcctactgt agcttgtcta ttctatgtat gcgtacgcgc
     2101 cggcctaggg aagtctgatg ggtattgctg gcagctcagg cactttggaa aactcttctt
     2161 ccatcctctg gtctactaat cccgaaacct ctcctatgaa gcgtacggct tgttcattag
     2221 ccgtctcaaa atttattgta aagccgccaa cttgacccgc atccacaagt atgccgctca
     2281 agagctcaga gataacgcct agctctccct ccatctctgg cacatatggc gttacggatg
     2341 atctaaggct ctttacaaca gccattgcag gactcaagat aatcactaga tcgccaaagt
     2401 ctgaagccaa cgatagcctt gatactagct tttccagcgc gacacatgat aggagtaggg
     2461 ctcttgatac ctgccttccc cttgcgagtt ctgaagaaag taaagttgaa tgctgtgtat
     2521 tattctcgtt aatggaaacc ttcaacttag tgaaaatgtc attgtctttt agcctgttca
     2581 tgctgtgaag ctcttccagc cggtcaattt gcacttgtat ctttttgctc acggcatcca
     2641 gccgcggctt tagcggccct ccaggcctgg gcaaggaagc cgtttgatta ttattattat
     2701 ctctgtcgtt gctatctagc actgattcag attttaccca tcttgaattc ataccgactg
     2761 gcttatgata tttagaataa tacatgctat gtatcgtaac gttttgtaac tgcggtaaca
     2821 agagcctata gtaacagctc agcatttttt tgaactttga cgcaatctat ttgaccaacc
     2881 gccttacctt tggttttcat tcggtaagaa tatgatgact gacactaata gtgatcttcc
     2941 cggtagcctt gaagaatttg gcctgtcaaa atatgaagct agggcatatc ttacgatgat
     3001 aggcaaggga tcgctagcgg taagcgagat agcttactat gccaatctgc cacgtaccaa
     3061 ggtctatcaa acagtgaaaa agctggaaaa gaagaagctt gccgtcatat ctaagcaaaa
     3121 gccactcgta tgcagcgcaa tcccacccga ggaggccttt gctgagatcg tcaacttaca
     3181 tgatcgtagg gtaaagaaca tgaggaatat agttgattct ttacaaaaga taaacgacga
     3241 gagccagcgc ccgaagggct ctgaggaaag gaggtatttc atactggatg caaacgctac
     3301 gcttgaaaag ataggtagcc taatagcaga ctcgcgc
//
LOCUS       DASQTF010000207         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1182885,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000207 DASQTF010000000
VERSION     DASQTF010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182885"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VE308_04940"
     CDS             <1..1365
                     /locus_tag="VE308_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HZB00348.1"
                     /translation="IPKIYIDYKDFAEARGMDPQKLEYGIGIKKMAIPDTNQDSACMA
                     ANACMKLMQKDHLHPEHIGRIYVATESSLDESKAMNSFVIGMLEQIYGEGSFEHAGGI
                     ECKFACVSGSYALYDNTNWIRADENDGKAAIVVVSDIAKYDIGSTGEYTQGAGAVALL
                     VKENPRLLAFDPKVPSTTIKNEYDFYRPFGKETPLVNGSYSNLLYLISVRKAFDAYKE
                     KAIRTGLIKLKDGESITDHIDFFAVHLPYRKMGEKALAYLLRHEWRHLPRWKNVIKEV
                     GMTEPQPKDPRGTIESILADTEFMKADEKFRRAFMQTSFYNEVYEKKMTSSLEASAII
                     GNLYTASMYMGLRSMLEFEFKKGIDLEQKRIGFGSYGSGSSAMVFSGTVQSQYKEIVS
                     KMDLDAEIGPRQKLTMREYERLHRNERDFNDSIVHSHKEFVLVKVGGNTAEKAGLREY
                     NYVS"
     gene            complement(1370..>2072)
                     /locus_tag="VE308_04945"
     CDS             complement(1370..>2072)
                     /locus_tag="VE308_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZB00349.1"
                     /translation="IYLPIGGGGLAAGVAIAVKSRKPNVKIIGVESKAFPAMKESLAK
                     GSLQSTKRGYSIADGIAVKQPGELTHQILSKYLEDIVLIDDTSIVKTMFLMMERAKLV
                     AEPAGAASLAYLLSDDGYEGSSKDKVATILSGGNVDMYLLGQVVAKGLMQMGRLLKIF
                     ILLPDKPGALKGVVDGIAELSVNIVEVEHDRLSAHIPAGTAGVYLSLELENEKHAHRL
                     IEFLTQKGIEFKIVS"
BASE COUNT          585 a          450 c          483 g          554 t
ORIGIN      
        1 atacccaaga tttacatcga ttacaaagat tttgctgaag caaggggtat ggatccccag
       61 aaactcgagt atggtatcgg gattaagaag atggctattc ctgacactaa ccaggattct
      121 gcctgcatgg ctgcaaacgc ttgcatgaag ttgatgcaga aagatcacct tcatccagag
      181 catatcggaa gaatttacgt cgccactgaa tcatcgctgg acgaatcaaa ggcgatgaac
      241 tcttttgtta ttggaatgtt agaacagatc tacggtgaag gctcctttga acacgccggc
      301 ggcattgaat gtaaatttgc ctgcgtaagt ggatcttatg cactctatga caatacaaac
      361 tggataaggg ctgacgaaaa tgatggcaag gcagccattg ttgtcgtaag cgacatagcc
      421 aaatatgata ttggttctac tggggaatac actcaaggtg ctggcgcagt tgccctcttg
      481 gtaaaagaaa atccaaggct tttggccttt gatcccaaag taccttcgac aacgatcaag
      541 aacgaatacg atttttatcg tccctttggt aaagaaactc ctcttgtgaa tggaagctat
      601 tcaaatttgt tgtacctgat atcggttaga aaggcatttg atgcttacaa ggaaaaggca
      661 attagaacag ggcttataaa gctcaaggac ggcgaatcga taactgatca catcgatttt
      721 tttgcagtgc accttccata caggaagatg ggtgagaagg cacttgccta tctgctaaga
      781 catgagtggc gccatctccc aaggtggaag aatgttatca aagaggtcgg gatgaccgag
      841 ccccaaccaa aggatcctcg tggaactatt gagtcaattc ttgctgatac tgaattcatg
      901 aaggctgatg agaagttcag acgcgctttc atgcagacat cattttacaa cgaagtatac
      961 gagaaaaaga tgactagctc gcttgaagct tctgcgatca ttggcaattt gtatactgca
     1021 tccatgtata tggggctccg aagtatgtta gagtttgagt tcaaaaaagg gatagacctc
     1081 gaacaaaagc gaataggctt tggctcatac gggagcggca gcagcgcaat ggtctttagc
     1141 gggacagtac agtcacaata caaggaaata gtaagtaaaa tggacttgga tgcagagatt
     1201 ggcccaagac aaaaactcac gatgcgtgag tatgaaaggc tccataggaa cgaacgcgac
     1261 tttaatgatt cgattgtcca ctcccataaa gagtttgtac tcgttaaggt tggcggcaat
     1321 actgccgaga aggcagggtt aagagaatac aactatgtca gttagttagc taactgacta
     1381 tcttgaactc tattcctttt tgtgttagaa attctatcaa gcggtgagcg tgtttttcgt
     1441 tttcgagctc aagacttagg taaacgcctg ctgttcctgc ggggatatga gcgctcaacc
     1501 tgtcatgctc tacttcgact atgttaacgc ttagctctgc gatgccatct actacacctt
     1561 taagcgcacc aggcttatct ggcagtaaga taaaaatctt aagcaacctg cccatttgca
     1621 tcaagccctt cgccacaact tgtcccaaga ggtacatgtc gacgtttcct cctgatagta
     1681 tggtggcgac cttatcctta ctactgcctt catagccgtc atctgatagt aggtaagcga
     1741 ggctggccgc tcctgccggc tcagcaacca atttggcgcg ctccatcatg agaaacattg
     1801 tctttactat cgacgtgtca tcaattagga caatatcctc taggtattta cttaatattt
     1861 ggtgcgtaag ttcgcctggc tgtttaactg cgataccatc tgctatgcta tagccacgct
     1921 ttgtagattg gagcgaacct ttagcaagcg actctttcat ggcaggaaag gcctttgact
     1981 cgacgcctat tatcttgaca tttggctttc tcgacttgac tgcaattgcg acgccagcag
     2041 caaggcctcc gccgcctatg ggcaaatata tc
//
LOCUS       DASQTF010000208         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1102616,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000208 DASQTF010000000
VERSION     DASQTF010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102616"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VE308_04950"
     CDS             complement(<1..260)
                     /locus_tag="VE308_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00350.1"
                     /translation="MSTTTSAAIYLVHLNPMTNAHESIISTLKKDYRVYIFPVRFIKE
                     GQEINTKSLPFSYQLRKQMVESIFGDSVAVLPDYTFYAPYSK"
     gene            complement(313..636)
                     /locus_tag="VE308_04955"
     CDS             complement(313..636)
                     /locus_tag="VE308_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZB00351.1"
                     /translation="MSEPSQPSQEQTTPRREGPHDTIYIGKKPLMTYVTSTLIQLANQ
                     PTVTIKARGLSIGRAVDVSQITLKRMENAGYKIGDVRIGSETVQSEDGRTRNVSTIEI
                     EIKKS"
     gene            760..1266
                     /locus_tag="VE308_04960"
     CDS             760..1266
                     /locus_tag="VE308_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00352.1"
                     /translation="MHTDNQLRLFGKREATNRWKAEYDDTLYKIYDWDSNLAGYMFPA
                     YSDIESNDEESIVEMNRIHAQVTEGTLLLPMVKLNLLDNNEGIDIDYVISSLEANAER
                     TNRWKKWLHDNAEPFNIAGSAVYTAREDRNMLSIALSIVETFNLGEKEVRDFLTPLLN
                     KLHEDGLL"
     gene            1305..>2109
                     /locus_tag="VE308_04965"
     CDS             1305..>2109
                     /locus_tag="VE308_04965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZB00353.1"
                     /translation="MKIQFAHHLVCLQHTDKQLRLHLESLEMEGSSECTEGFLRCKEC
                     DTRYPIIQGVAVVVKDFFAYAAGRPQIYGRWLLDSKTKEMKQFLKESGSNFSPGSASN
                     DRYEEGGSWFIPYRWTQYEHSIEDRLLKSLRWRLKPNEVYNRIVHSINPKMDGVALDM
                     ACSMGYSAMLLAQKYAFVIGIDISFSFIREARKRMYELKRGNIEFCVSDLLNTPFNPS
                     KFDLILALNLLELVDPEKLLASIHWLLKPHADMIIVDPYDYNRDPPAEKI"
BASE COUNT          593 a          441 c          506 g          569 t
ORIGIN      
        1 tacttgctgt atggcgcgta gaaggtataa tcgggaagaa ctgcgacaga gtcgccaaag
       61 atagattcaa ccatctgctt gcgcagctga tacgaaaacg ggaggctctt tgtgtttatc
      121 tcctgccctt ctttaataaa acgcactggg aaaatgtaga cgcgatagtc ctttttgagc
      181 gtcgagatta ttgattcatg agcattagtc attgggttta gatgcacaag atagatcgca
      241 gcggaggtag tagtgctcat gatttaactt agatagtaca agagatagta agaaaaagta
      301 aaagaagcag gcctaactct tctttatctc gatttcaatc gtagagacgt ttctcgtcct
      361 gccgtcctca gattggactg tctcagatcc aatccttacg tcgcctatct tgtatcctgc
      421 attttccatc ctcttcagtg tgatctgcga tacatctact gctcgtccaa tgctcaggcc
      481 ccttgccttg attgtcactg ttggctggtt ggccaactga atcagagttg acgtcacata
      541 tgtcatcagc ggtttcttgc caatgtatat tgtgtcgtgg ggtccttctc tgcgtggggt
      601 tgtctgttct tgtgatggtt gtgatggttc tgacatgcat gggttaaagc ctcagggata
      661 atttaagtca acggtcaata aagcatctct ctttaactct aaccttaacc ctatagtgat
      721 tatcactagt gatgtatcat agagagaaat atgtaaggca tgcatactga taatcagttg
      781 cgtttgtttg gcaagagaga ggccaccaac aggtggaagg cagaatatga cgatacgctc
      841 tacaagatct atgactggga tagcaacctt gcaggttata tgttcccagc atacagcgat
      901 attgagtcca atgacgaaga aagtattgtt gagatgaata gaatccacgc ccaagtcacg
      961 gagggaacac ttttgcttcc gatggtcaag ctgaacctgc ttgataacaa tgaaggaata
     1021 gacattgact atgtcatttc ttcacttgaa gcaaacgcag agagaaccaa caggtggaaa
     1081 aaatggctgc acgataacgc tgaaccattt aatattgcag gttctgctgt gtatacagca
     1141 agagaagatc gaaatatgct ttcaatagcg cttagcattg ttgaaacgtt caatctaggc
     1201 gaaaaggaag tgcgcgactt tttaactcca ttacttaaca agctgcatga ggatgggctt
     1261 ctctgatttt tatatatatg aaggcgctcc tgtgatatgc aagcatgaag atccagtttg
     1321 cacatcacct cgtctgtctc caacacacag acaaacaact gcgtctccac cttgagagcc
     1381 ttgagatgga ggggtcaagc gagtgtactg agggattcct gagatgcaaa gagtgtgaca
     1441 caagataccc cataatacag ggcgtggcag tagtagtcaa ggacttcttt gcctatgctg
     1501 caggccgacc gcagatatac ggcaggtggc ttctggacag caagacaaaa gagatgaagc
     1561 aatttttgaa ggagagcggc tcaaactttt cgccgggctc tgcatcaaac gacagatacg
     1621 aggagggggg cagctggttc atcccttaca ggtggactca gtacgagcat tctatagaag
     1681 ataggctgct caagtcactc cggtggcgat tgaagccaaa tgaagtttac aacagaatag
     1741 tgcatagcat caaccccaag atggacgggg ttgcacttga catggcatgc tcaatgggct
     1801 atagcgctat gctccttgcg caaaagtatg cgtttgtaat tggcatagac atctcctttt
     1861 cattcatcag ggaagctaga aaaaggatgt acgaattaaa acgtggaaac attgagtttt
     1921 gtgtgtctga tttgctgaat acgccgttca accccagcaa gtttgacttg atacttgcat
     1981 tgaacctgct cgagctcgtg gatccggaaa agcttctagc gtcaatacac tggctgctta
     2041 aaccgcatgc cgatatgata attgttgatc cgtatgatta taaccgcgat cccccagcag
     2101 aaaagatct
//
LOCUS       DASQTF010000209         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_732002,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000209 DASQTF010000000
VERSION     DASQTF010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732002"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VE308_04970"
     CDS             <1..358
                     /locus_tag="VE308_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZB00354.1"
                     /translation="KPKERKEILESWGKDYFPLLSVHTLEIGYDVPQVGIAIIIASTS
                     NMNQVAQRIGRVVRKVDGKDQALVYVVYVSGTKDDNVLKVVRAAVEKEDERTATVRVP
                     KGKRPTHGQKTITKFS"
     gene            complement(353..1009)
                     /locus_tag="VE308_04975"
     CDS             complement(353..1009)
                     /locus_tag="VE308_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00355.1"
                     /translation="MDLQRFVADVTSLSGKMSAGEPEEIVERMNFVKNRLIELYMRNL
                     VKINHSAMELVCAKHLIRYGYKVDVEKQLTDILICDVYAEKGDGAAIVEIETGFIPPE
                     HALDPLSYYAARIASKIARYSKYANQFVLATPPVSVLPIPALFRRPPRDRRPSEIKKV
                     KVLCDKYYKNPPVTEDEILNGRLHITYIINIDAGKVVEMDIDSYFEHVGGMLSTCMDL
                     "
     gene            1098..2249
                     /locus_tag="VE308_04980"
     CDS             1098..2249
                     /locus_tag="VE308_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZB00356.1"
                     /translation="MSLSTINKITILGSGIMGHGIAQVSAMAGYNVDLRDIEKSFLDK
                     AMDKIKWSLNKLVEKQKLSQTEADKIFARITPVVDLEQALKGADLLIEAVPEDMNLKR
                     KVYAEVDSFAESRTLYASNTSTLPITEMAALTSRPDRFIGLHFFNPPQLMPLVEVIPG
                     DKTNQSMIDMATGLVQRLGKQPVLCKKDVPGFIVNRIFIPLVHEAVYCQERDNVPMTT
                     IDSAVKFKLSFPMGIFELADYSGLDVIHKATVEMHSRDKKVIQPHPKIKQLFDEKNLG
                     QKSGKGFFEYKGDKYERINLTEQEAAKYDPIALIGVAANNSAWLISNRVCSKEELEKA
                     LKLGMGLKKELFTTVQEFGVKNIVRSLQDLALKYGSYYDPDPYLVNYQD"
     gene            complement(2694..3206)
                     /locus_tag="VE308_04985"
     CDS             complement(2694..3206)
                     /locus_tag="VE308_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HZB00357.1"
                     /translation="MRKLQIAVIGYNKDRCTDIASKMAYEVGKEIACVGAVLICGGLG
                     GVMEYACKGAKEYGGSTVGIIPQEEFSYANEYCDIVICTGIGYARDFVVASSADGIIA
                     VGGGIGTLIELGVGYMTKKTMVAISGSGGVADIYGGKFLDERNRVSITVAKDAKTAVE
                     MILATTVKAR"
     gene            3687..>4007
                     /locus_tag="VE308_04990"
     CDS             3687..>4007
                     /locus_tag="VE308_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZB00358.1"
                     /translation="MKFLDRIDAGNLLAERLAENGYTHAIVLGIPRGGVILADIVAKK
                     LAADFDIVIPRKLGAPENEELAIGAVMEDGTSYINSYIVSALRIPQNYIESERVRQAA
                     EIRRR"
BASE COUNT         1199 a          870 c          879 g         1059 t
ORIGIN      
        1 caaaccaaag gagagaaagg agatcttgga aagctggggt aaagattatt ttcctttact
       61 gtcggtgcac acgcttgaaa ttgggtatga tgtgccgcag gttggcattg caataattat
      121 tgccagcacg tctaatatga accaagtcgc acagaggatt ggacgggtcg taaggaaagt
      181 agatggaaag gatcaggcgc ttgtatatgt agtgtacgtt agcggtacca aagatgacaa
      241 tgtgctaaag gtggtcaggg cggcagttga aaaggaagat gaaagaactg cgactgttcg
      301 cgtcccaaag ggcaagagac ctacgcatgg tcaaaaaact atcacaaaat tctcataagt
      361 ccatgcaggt agacaacatg ccgcccacat gctcaaagta tgaatcgatg tccatctcga
      421 ctaccttacc tgcatcaata tttatgatgt atgttatgtg cagccggccg tttagaatct
      481 catcttcagt cacaggtgga ttcttgtaat atttatcaca tagtacttta acttttttga
      541 tttcacttgg tcttctgtca cgaggcggac gtctgaacag ggcaggtatc ggaaggacgc
      601 tgacaggtgg agtagccaag acgaactgat ttgcatactt gctatagcgt gctatcttgc
      661 ttgcaatcct tgcagcatag tatgacagcg gatcgagtgc gtgctctggt ggaataaagc
      721 ctgtctcgat ttcaacaatg gctgcgccgt ctcccttttc cgcgtacacg tcacagatca
      781 aaatgtctgt cagctgcttc tcaacgtcaa ctttatagcc gtagcgaatt agatgcttgg
      841 cgcatacaag ctccatcgct gaatggttta ttttgacaag gtttctcatg tagagctcga
      901 tgagtctgtt ctttacaaaa ttcattctct ccactatttc ttctggctca ccggctgaca
      961 tcttaccaga gagcgacgtc acgtctgcaa caaagcgctg tagatccaat tatcagaaca
     1021 ttatagtcaa gtatggtttt aaagatgatg ctcattagca acattaaaaa ttacccaaga
     1081 gccatgctta tgtcataatg tcactatcaa caatcaacaa gataaccatt cttgggtctg
     1141 gtattatggg ccacggtatt gctcaggtat ctgcaatggc aggctacaat gtagacttgc
     1201 gtgacataga gaagtctttt cttgacaaag caatggataa aataaaatgg tcgctcaaca
     1261 agcttgttga aaaacagaag ttgtctcaga cggaggctga taagatcttt gctagaatca
     1321 caccagtagt tgacctggag caggctctca aaggtgcaga cctgctcatt gaagcagtac
     1381 cagaggacat gaacttgaaa aggaaggtat atgcagaggt tgacagcttt gcagagagca
     1441 ggacgcttta tgcttcaaac acaagcactc ttccaatcac agaaatggct gcactgacaa
     1501 gcaggccaga tcgctttatc ggccttcact ttttcaatcc accacagctg atgcccttag
     1561 tcgaagtgat acctggcgac aaaaccaacc aaagcatgat tgacatggca acaggtcttg
     1621 tgcagaggct tggtaagcag ccagtgctct gtaaaaaaga tgtccccggc tttatagtca
     1681 accgcatctt tataccgctt gttcatgaag cggtctactg ccaagaaaga gacaacgttc
     1741 caatgactac tatcgactcg gcagtcaaat tcaagttatc atttccaatg ggaatattcg
     1801 agcttgcaga ctatagtggc cttgacgtga tacacaaagc aaccgtcgag atgcattcta
     1861 gagacaagaa ggtcatccaa ccccatccca agataaagca actgtttgat gaaaagaatc
     1921 taggccaaaa gtcaggcaag ggcttttttg aatataaagg tgacaagtac gagcgcataa
     1981 acctgacaga acaagaggct gcgaagtatg atccaattgc actgatcgga gtggctgcca
     2041 acaactcggc gtggcttatt tcaaacagag tatgcagcaa agaagaattg gaaaaggcgc
     2101 tcaagcttgg tatggggttg aaaaaagagc tgtttaccac agtacaagaa tttggggtca
     2161 aaaatatcgt tcgcagtctg caagacttgg ctttaaaata cgggtcgtac tatgatccag
     2221 atccatactt ggtcaactat caagattgat agtccttcct acagtagcca acttcttagg
     2281 aacatttagt tatactttaa aaaaattagg gttatagaaa cttttacatt cgaatgtata
     2341 agattttctc atattcagat gaaagactca gagaattcag ggatacatca tgaagattac
     2401 ggaaatataa gacgattaat ctctacatcc tcgagtctaa attgttagcg cttgttggac
     2461 tgtcgctgtt tgccagggca ggaagtacac cctagtagag tattgcatga aaagaatgca
     2521 atgctccata cggagcatta acaaatgtct gcaaaaattt tctccttcct taacttctac
     2581 aagcaatagg gactcactct tcctatcagg gcaacaagcc aacaggtgct gatttatact
     2641 atctattgat ctgatagcct tgtatctatc ttaaactcca gttctggttt acatcatctt
     2701 gctttcacag tagtagcaag tatcatttct actgcggtct ttgcatcttt tgcaacagtt
     2761 atcgagaccc tgttacgctc atccaaaaac ttaccgccat aaatatctgc tacgcctccg
     2821 cttcccgata tcgctaccat tgtcttctta gtcatgtagc ctacacctag ctcaatgagc
     2881 gttcctatcc cgccacccac tgcgattatt ccatcggcag aagatgcaac gacaaaatct
     2941 cttgcgtagc ctatgccggt gcatatcacg atgtcacaat attcatttgc gtacgagaac
     3001 tcctcctgtg gtattatccc aaccgtgctg ccgccgtatt cttttgctcc cttgcatgca
     3061 tattccatca caccaccaag ccctccacag atcagcacgg caccaacaca tgcaatttcc
     3121 ttccccactt catatgccat cttgcttgca atgtcggtac atctatcctt gttgtagcca
     3181 atcacagcaa tctgtagctt gcgcacaggc cagagcaata atcatcgtct gaattatagt
     3241 tttcgcaaat agatgtttta cgacacagat aagaaaaaat aaaataaaaa gtgaaacaaa
     3301 agtacaagat attgccttca tagtccataa ttctacttgc tttggtaata acggtgacaa
     3361 aaaaggttgt gctgattcca ctcaaattga gggcgatacc agaacgtcat ggataggaca
     3421 gtataatgct ccaaaatatg ccctaaaaga gatgttcagg ccagtcctgt gcggagctgc
     3481 aatcagttga aagttaagcc ttaagggtaa atagacaatt caagttttgt gtttgctttc
     3541 ttttccaagc ggagagaggt aggaagaggg cctagagggt cttgttacga tggaggctcc
     3601 aatattgaat gggctcctgc caattcctgc aattgcaaat gctgataacg atcaagactt
     3661 aaaactgtaa gaagagaaat catgttatga agttcctcga taggatcgat gcaggcaatc
     3721 tccttgcaga aaggctggcg gagaatggat atacccatgc gatagtactt ggcattccgc
     3781 gcggaggcgt catactagcg gatattgtag ctaagaagct tgccgcagac tttgatattg
     3841 tgataccaag aaagcttggg gcgccagaga acgaggaact tgcaatagga gcagtgatgg
     3901 aggatggtac ttcatacatt aatagctata ttgtcagtgc gctgcgaata ccacagaact
     3961 atatcgaaag cgaaagggta cgccaggccg cagaaatcag gcgtaga
//
LOCUS       DASQTF010000210         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_455620,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000210 DASQTF010000000
VERSION     DASQTF010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455620"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /gene="tfb"
                     /locus_tag="VE308_04995"
     CDS             <1..372
                     /gene="tfb"
                     /locus_tag="VE308_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020700.1"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HZB00359.1"
                     /translation="KATNTKEKDLARAYRILLRELDLKVPMLDPIKCVSRVANKANIS
                     ERTKRRAMDMMLDVIKSGQAAGKDPMGLAASVLYLACVSSSEPKSQATIAEAAGVSEV
                     TLRKNQRMILDMRQQPAETLL"
     gene            complement(431..1222)
                     /locus_tag="VE308_05000"
     CDS             complement(431..1222)
                     /locus_tag="VE308_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZB00360.1"
                     /translation="MMIVDILKEACQQVHEKTKGLAGTTVGSKEMGRGAGGDISRTID
                     LTAENTVIDIIKKQGIEATIIGEECGRIEGKQGYIIIDAIDGTTNAICGIPFFCCSVA
                     YATDFYLSAVADAAIIDLARGDLYYASKNQGAFLNGKRIFVKRERTPDSTIGINVSGI
                     KPAMVERLAPIIAEATHVRQFGANALEMCFVARGFLDAYIDLRGKIRPTDVAAGYLIA
                     KEAGAKIYSDDGSTLESNLDVKTRFSYVAVSNDKIREQLVTLFLR"
     gene            1520..2407
                     /locus_tag="VE308_05005"
     CDS             1520..2407
                     /locus_tag="VE308_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZB00361.1"
                     /translation="MLTTPAPSYSHNYDANLMERLLFRVAKRWVAGYSADEAINAALD
                     TSSHGMSAILNFLGEDTTNAKLVDMTVNEYLLLMDLMNARKVHGCVSVKPTQLGLAID
                     YNMCLQNFSGIAARARELGQFIWLDMESVKSTEDTITIYLDLYKQYDSIGVAVQSYLR
                     RSASDLLHIIECGGKVRLVKGAYHESEEHIFVTNEQVNANYSKLMKMLFESNNYFAIA
                     THDSALVEEAISMGKNTKFEFQMLKGIRDELKLELVTKGFAVAEYIPYGSQWLPYSVR
                     RIMERRRNLLLLARSFIQQ"
     gene            2882..3319
                     /locus_tag="VE308_05010"
     CDS             2882..3319
                     /locus_tag="VE308_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00362.1"
                     /translation="MRQEHVTLQGVEISQLYDQIRNYLKDKKLDIINEEKEDNFWDIK
                     AHKGTKGSIIIGNVRDVEVMISGREGNYDLILRTGAWGKDIVVPAVIAGVLTAGVGAV
                     LAGGASAYRAHAFEKHFWDFVQKTLAEIGKGKSAMSEPVTVTP"
BASE COUNT         1060 a          682 c          775 g          931 t
ORIGIN      
        1 aaagctacaa acacaaaaga aaaagacttg gcaagagcct acaggatact tttgcgtgag
       61 cttgacctca aggttccgat gcttgaccca atcaagtgtg tttcccgagt agcgaacaag
      121 gcgaatatca gcgagcgcac aaagcgtcgg gcaatggata tgatgcttga tgttataaaa
      181 agcgggcagg cagcaggcaa ggatccgatg gggcttgctg cgtcagtatt gtatctcgct
      241 tgcgttagca gtagtgagcc aaagagccag gcgacgatag cagaggcagc aggtgtatca
      301 gaagtaacat tgaggaagaa ccagcgtatg atattggata tgaggcagca acctgcggag
      361 accttgttat aaatcaaata tttttttatt tttaaaataa gcactaaaat gatgcaatat
      421 ctactatatc ctatcttagg aatagggtta ctaactgctc acgtattttg tcatttgaca
      481 ctgcgacata cgagaatctc gtcttgacat cgaggttaga ttcaagggtc gatccatcgt
      541 ctgaatatat cttggctcca gcctcttttg ctatcaggta gccagctgcg acatcagtcg
      601 gccggatttt acctcgtaga tcaatatagg cgtccaagaa tcctcgtgca acaaagcaca
      661 tttccagcgc atttgcgcca aactgtcgga cgtgagtagc ctctgcaatg attggggcaa
      721 ggcgctcaac catcgcaggt ttgattccag atacatttat cccaatcgta gaatctggag
      781 tgcgttctcg cttaacgaag atcctcttgc cgtttaaaaa tgccccttga ttctttgaag
      841 catagtagag gtcgcccctt gcaagatcaa ttatggctgc atcagccacc gcgctcaggt
      901 aaaagtcggt tgcatatgcg accgagcaac agaagaaagg tatcccacaa atggcgtttg
      961 tagttccatc aattgcatct ataatgatgt agccctgttt gccctcaatc ctaccacact
     1021 cttcgccaat tatcgtggct tcgatacctt gctttttgat aatatcgatg acggtatttt
     1081 cagctgtcaa atcaattgta cgcgatatat cgcctcctgc acctcttccc atctccttgc
     1141 taccgacggt agtcccggca agaccctttg tcttctcatg aacctgctga catgcctcct
     1201 ttagaatatc aactatcatc attgcagcat ccttaagata gactcaataa aagattatgt
     1261 ccatgcatga aagagacaca agttacgaga taatctaatt cttatcttgc aacatacaag
     1321 aatacctttc atatcaaatt gtcctcattg tggccgtaat tatgaaagtg ttgatccata
     1381 aaaaatattc tgacagcaac tcgcggcttc aatgctgttg acaggtgaag ctcttttatg
     1441 caatatgtat tttacatgtt taaggaaaac cattcgtcta atgtctaaga ttatatcgta
     1501 gagctgacaa taatatatca tgctgactac tcctgccccc tcctattccc acaactacga
     1561 tgccaatttg atggagcgat tgttattcag ggtggcaaaa aggtgggttg caggttatag
     1621 tgctgacgag gcaataaatg cagcgcttga cacaagcagt cacggaatgt ctgcaattct
     1681 aaactttctt ggcgaggata ccacaaatgc caaactggtt gacatgacag ttaacgaata
     1741 cctgttgcta atggacctga tgaatgcaag aaaggtgcat ggttgtgtgt ctgtcaaacc
     1801 tacgcagctt ggccttgcaa tcgactataa catgtgtttg cagaatttca gcgggattgc
     1861 tgcaagagct agagagcttg ggcagttcat atggttggac atggagtcag tcaagtctac
     1921 agaagacaca attaccatct atctagactt gtataagcaa tatgatagta taggcgtagc
     1981 agtacagtcg tatctgcgcc ggagcgctag cgacctttta cacattattg aatgcggcgg
     2041 taaggtaagg cttgtcaagg gcgcatatca cgaatcagag gagcatatct ttgttacaaa
     2101 tgaacaagtc aatgccaatt attcgaagct gatgaagatg ctctttgaga gcaacaatta
     2161 tttcgcgatt gcgactcatg actcagctct agtcgaggag gcgatcagta tgggcaagaa
     2221 taccaagttt gaattccaga tgctgaaggg tattcgagac gaactaaagc ttgagcttgt
     2281 tacaaaaggg tttgcggtgg ctgaatacat cccttatggt agccaatggc ttccctattc
     2341 ggtgcgaaga ataatggagc gaaggcgcaa cttactgcta ctagcaaggt cgttcattca
     2401 acaatagcgg gccactaaag cactactatc accctctggg gcccataaaa aagtaaatag
     2461 aactacaaaa cccatatcct cgatgaatga aatcaaatta gtcatttttg gattgataac
     2521 actgttaatt gctgcgatat tgttgtgggc tttgggcttt agacgaacca gaaatactag
     2581 tagacaagga agtagagata acaatagcat taggggtggg attattgatc ttaattctag
     2641 atagaagagc tgaccgccgt atccatgaaa ccattcatta acaacatcta ttgattaata
     2701 aaatgaacaa aaaaattgat gaataaaatg agttaatcaa aaatatacga aaaattaccg
     2761 tcaagtcctg gagatgatgt cagcaataaa gagaaaaagc tatgatgttg gatgtccaga
     2821 ctattgcgat tgttgcatac acagccatct cctttaaata gttccatttt catcaatatg
     2881 tatgagacag gaacatgtaa ccctacaggg tgtagagatt tcacagctat acgaccaaat
     2941 caggaactat ctcaaagata agaaactaga cataatcaat gaagagaagg aagataattt
     3001 ttgggatata aaggcacata aaggtactaa aggtagtatt atcattggaa atgtacgaga
     3061 tgttgaggta atgataagcg gccgcgaggg taattatgac ttaattttac gcacaggtgc
     3121 ctggggtaag gatattgtag ttccagcagt tatagcaggt gttctgactg ccggagtagg
     3181 tgcagttctt gcaggtggag cttctgcgta tagagcacat gcatttgaaa aacatttttg
     3241 ggattttgta caaaagacct tagctgagat aggtaaaggt aaatcagcga tgtctgagcc
     3301 tgtaacagtg actccttaag ttaggactgg aaggagtaca tattaggctc attagataaa
     3361 gctaaaaaac tgaacagtgt ggtagtattt acgaggcaaa aggctggatt gtgagctcca
     3421 gagagcaatg tatcgaatga caacttgt
//
LOCUS       DASQTF010000211         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1002940,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000211 DASQTF010000000
VERSION     DASQTF010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002940"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..607
                     /locus_tag="VE308_05015"
     CDS             359..607
                     /locus_tag="VE308_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00363.1"
                     /translation="MAEGKPPVIDWDRVVNKNVRTKEGEPIGNIVAHMKDSIQIETTG
                     ARGQYMVPKEHVEGFDGAEVTLNLTVDELAKFVLGGGP"
     gene            complement(777..1955)
                     /locus_tag="VE308_05020"
     CDS             complement(777..1955)
                     /locus_tag="VE308_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZB00364.1"
                     /translation="MSLQIAKVKGIPVRLHFTLIIVFFLIAWTLASSFMPQYFPNLTT
                     VQYWIMGATGAIILFISVFLHELMHSVMALRYGIKVRQIILFIFGGVSEIPEESRDFR
                     KEFKIAIAGPAASFAIAGTLAVSWWLVSQIAPTEQQLLATKQVIEGILLYGAIINTML
                     GGFNLIPAFPLDGGRILRAGLVAWKKDYDTATRIAARVGIAISYGFLGIGFLTMISGS
                     FISGIWILLIGWFVNSGAQSYLSQHELTSVLAGVRLRDIMNTRVIAVRKDVKIDELLK
                     EYFATYMKSSFPVIDYSGQMLGMVTLKRVQETQEDKRHQLTAGEIMIPLEDLVVMLPS
                     RSADEALMKMTRTRMGKVFVCDLQGVLLGLVSKTDIMNLASERQEFQQELRKFASTGS
                     "
     gene            2236..2766
                     /locus_tag="VE308_05025"
     CDS             2236..2766
                     /locus_tag="VE308_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZB00365.1"
                     /translation="MLAVTTYILNPIVPVLHLRYLQVSEVALVGFLAIHTISKISYSL
                     SIAYSEQTTKSIRSLIRIAGAIIVIAVVISYLSQDPVVAASISTISGLVIGFAASNLI
                     GNVIAVIYLAITRPFRIGDRIKVFNGDGRVSDIGLLYTRLLLDNGDEMLASNSSMVTT
                     NIILRKKEKGEENQEP"
     gene            complement(2988..>3398)
                     /locus_tag="VE308_05030"
     CDS             complement(2988..>3398)
                     /locus_tag="VE308_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00366.1"
                     /translation="QIAFYLSPIEDFRVNNEVEKKYDLVTAFECIHDMPYPVKALERM
                     HEMVALDGAVLVADELVGETVEENNGSFLGQLYYNFSVLHCLPQAMVYPDSAATGAVM
                     SASKLEGYAKKAGFSRVDILSIDNPLWRFYRLSP"
BASE COUNT         1021 a          709 c          692 g          976 t
ORIGIN      
        1 gtagtataca ggagaaaatt gaacaagagc tagcagaact tggagtaaat accacacagg
       61 cacaatatac aatagagagt agggcgacac aagtaggaag atcatttgca gatgaagcta
      121 tgggcgtatt taataatatc accaactcga taagagatat tatggtcata gaataagctt
      181 agatgcctga aaaccacgat acaccctgta ggatttcgca tatacaacat ttagacttta
      241 tcaacacgtt tgcaatgcgg aatgatggag aggctgacat gtggcattgg tatactagcc
      301 catcttctgt aatggtcatt cataattcta aaatgactta tgagtgaaga acaatgcaat
      361 ggcagaaggt aaaccccctg taatagattg ggacagagtt gtgaacaaga atgttagaac
      421 caaagaaggc gaacctatag gcaacatagt tgctcacatg aaagattcga ttcaaataga
      481 aactactggc gcaagaggac agtatatggt accaaaggaa catgtagagg gatttgacgg
      541 agcagaagtt actctgaatc tgaccgttga tgaattagca aagttcgtac ttggcggtgg
      601 gccttgatct attttctaaa tttctacacc tgtttattgt ttcaactcac ctcaagcaac
      661 aattttgatt agaatgttgg tccttcctcg aagcgatata gcaataaaac ctattactct
      721 taaccagcta tatctatccc ccattgcaat tgatgataaa gttagctaca tgaagcttaa
      781 gaaccagttg aggcaaactt cctgagctct tgttgaaatt cttgcctttc acttgccaaa
      841 ttcatgatgt ctgttttgct cacaagtccc aacaggacac cttgcagatc gcagacaaac
      901 acctttccca tgcgtgtacg tgtcatcttc atcaatgctt cgtctgcact tctgcttggc
      961 agcatgacca ctagatcttc taaaggaatc attatttctc ctgctgtcag ctggtgcctt
     1021 ttgtcctctt gtgtctcctg tactctcttc aaggtgacca tgcccaacat ttggccactg
     1081 tagtctatca ctgggaaaga gctcttcatg tatgttgcaa agtattcttt tagcagctca
     1141 tctattttga catccttcct tacagctatg actcttgtat tcatgatgtc tcttagccgt
     1201 acgccagcca gcaccgatgt cagctcatgc tgagaaaggt aagattgtgc cccgctatta
     1261 acaaaccatc ctattaacag aatccaaatt ccactgatga atgaccctga tatcatggtc
     1321 aagaatccta tgcccagaaa gccatacgat atggctatcc cgactctggc tgcaatcctt
     1381 gtagcagtgt catagtcctt tttccatgca acaaggcctg cacgcaatat tcttccacca
     1441 tctagtggaa acgcaggtat caaattgaat cctccaagca tggtgttaat aatagctcca
     1501 tacagcagaa tcccttcaat aacttgcttt gtggccagta actgttgctc tgtaggagca
     1561 atttgagata caagccacca agatactgca agggttccgg caattgcaaa gcttgctgcc
     1621 ggccctgcta tggcaatttt gaactccttt ctgaagtctc tactttcttc aggtatttca
     1681 gagacgccgc caaaaataaa caggatgatc tgccttacct tgataccata gcgcaatgcc
     1741 attacagagt gcataagctc gtgaaggaat actgaaatga aaagtatgat tgcgcctgta
     1801 gcccccatta tccaatattg cacggttgta aggttgggaa aatattgtgg cataaagctg
     1861 ctagcgagtg tccatgcaat caagaagaaa actatgatta gagtaaagtg taatcgcacc
     1921 ggtattccct tgacttttgc tatctgaagt gacatggggt acttagaaat gcatcgtatg
     1981 acttatagaa attgcatgaa acgcaatata gaatctctgc tgtaaattta atacatacgg
     2041 cactatcacg cacagattga aaaagtatat gaatactgaa aatgccttct tcttctagag
     2101 tagacggctt tctgactatt ataataatga ggaaagccca agccggcgac aagaggggag
     2161 ggagttagta tagaaaaaag agccaaggag tcagcgatag gatggtcagt cattcttgct
     2221 gcatccctta ccgtcatgtt ggcagtgact acctacatac taaaccctat tgttccggtc
     2281 ttacatctga gatatcttca agtcagcgag gttgctctgg taggcttcct tgcaatacat
     2341 acaatcagca agatttccta cagtttgtcg atagcatatt ctgaacagac aaccaaatct
     2401 attagaagtc tcatcagaat tgctggcgca ataattgtga ttgccgttgt aatttcgtat
     2461 ttgagccaag accctgtcgt agcagcatca atcagtacca tatctgggct cgtaattggt
     2521 tttgctgctt caaatttgat tggaaatgtt attgcagtga tatatctggc aataacacga
     2581 ccatttagaa ttggtgatag aatcaaagtg ttcaacggag atggaagagt gagtgatatt
     2641 gggcttcttt atactagact cttacttgat aatggagatg agatgttggc atctaactcg
     2701 tcaatggtga ccactaatat aatattgaga aaaaaggaaa aaggagaaga aaatcaagaa
     2761 ccataggggg aatatttgtt tgtaaagtaa tattgcagaa cacggactta agacaatttt
     2821 aggctgcagg agatctctgc agattatagc atcacttcat ccttttcttt atctctgcat
     2881 gtcctatctg cgtagtttct atctcttctt agagaggaag agtttgacgt acgtgcgagc
     2941 tatgtgcttc gagatattca aaatagcttc acctgtcctc tctattatca gggcgataga
     3001 cgatagaatc tccatagagg attatctatt gaaagtatat cgactcttga aaatcctgct
     3061 ttcttggcat agccttccaa ttttgatgct gacattacag cgcctgttgc agctgaatcc
     3121 gggtatacca ttgcctgagg caaacaatgc agtacactaa agttgtagta aagctgccct
     3181 agaaaactac cattgttctc ctctactgtt tcgccgacta gttcatctgc gactaaaact
     3241 gcaccgtcca gagcaaccat ctcatgcatt ctttcaaggg cttttacggg atacggcatg
     3301 tcatggatac attcaaaagc agtgaccaaa tcatatttct tttctacttc attattaaca
     3361 cgaaagtctt cgatcgggga aagatagaat gcaatctg
//
LOCUS       DASQTF010000212         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_736379,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000212 DASQTF010000000
VERSION     DASQTF010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736379"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VE308_05035"
     CDS             complement(<1..620)
                     /locus_tag="VE308_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZB00367.1"
                     /translation="MVLEVRKPRPLWEILFSTHHTDIGLLYIIFSLTLFFLGGLLAIS
                     IRTELFLPGAQLFEDANTFNRVFTVHGTTMLFLWVIPFAAGVGNYLIPIMVRYKDMAW
                     PKLNAVAFWMIPVGAALVWLGFADTGWTAYPPYSILRAPGPAAEMWIFGLKILGISSI
                     LGAINFVVTILRMKHPDLPMMKLPLFTWSILSTSLIILMSMPTFAAA"
     gene            complement(767..1276)
                     /locus_tag="VE308_05040"
     CDS             complement(767..1276)
                     /locus_tag="VE308_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00368.1"
                     /translation="MGHGTLEWVYIGVVVAVLIYIGADAWNVERFLDHAPEEAQVVKV
                     TAQQWFWTFEHEDGRRELGELHLTMGTPYRFEVTSLDVNHSFNTPDFAMMIDAIPGRI
                     NSIWYVPDQAGEFLIQCREYCGFSHYQMRAKLFVEEPAGGEELGAQVTNASATNNLIP
                     PLGAAAAAS"
     gene            complement(1276..1554)
                     /locus_tag="VE308_05045"
     CDS             complement(1276..1554)
                     /locus_tag="VE308_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme transporter CcmC"
                     /protein_id="HZB00369.1"
                     /translation="MVSYSTIVLILIPVATLTLLGVDSTAFAQETTERYLSARIEIWA
                     LFYRLMIIAFVIGAVVMGTMTYVIWRFRESNPKNRGEDITPSAGGIHH"
     gene            complement(1629..>2159)
                     /locus_tag="VE308_05050"
     CDS             complement(1629..>2159)
                     /locus_tag="VE308_05050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZB00370.1"
                     /translation="GRQQVMDSAKQLMKNIAIQKVYVSPVIRTVETAQIVCQILGTDY
                     VIDDRLYETEMGRLVGMNFEEITTKYGDVLTRFYSDYDPVLESFGVEAFASVKHRITS
                     LLDDLLQKHEDSNVLMVTHLDPIKAALATLLDLSPEALHRWNIRNASLTILKHESRIY
                     SISGVNIMVMHRYPAE"
BASE COUNT          601 a          509 c          449 g          600 t
ORIGIN      
        1 gcagcagcaa aggtcggcat agacatgagg attataagag atgtagaaag gatggaccaa
       61 gtgaaaagag gtagcttcat catcggcaga tcaggatgct tcattctcaa gatagtaacg
      121 acgaagttga tggcgcctag aatcgatgaa attcccaaaa tcttcaggcc aaatatccac
      181 atttcagccg caggccctgg tgctcgcaag atagaataag gaggatatgc agtccagccc
      241 gtgtctgcaa agccaagcca taccaaggcc gcgcctactg gtatcatcca gaatgcaact
      301 gcattaagct ttggccatgc catatctttg taccttacca taattggtat caggtagtta
      361 ccgactccag cagcaaatgg tattacccac aggaacagca tagttgtgcc atgaacagtg
      421 aaaactcggt tgaatgtatt agcatcttca aacagctgtg caccgggtag gaaaagttct
      481 gttctgatcg aaatagcaag tagtccacct aagaaaaaga gagttaagga aaagataatg
      541 tatagcagtc ctatatccgt atgatgagtc gaaaacagga tctcccacag cgggcgtggt
      601 tttctaactt ctagaaccaa ctcgcaccat ccccctctct atactgccca tacattgaag
      661 acaatatcaa taaaagcatt ttgttttagc acatcggtag tatgcaagct ctttatcatt
      721 ttattcattg accaagatat cgcacaataa ctcgtaaaaa gagaactcaa ctcgcagctg
      781 ctgcggcgcc taatggagga atcaggttat tagtagcaga agcattagtc acctgcgccc
      841 ccagctcctc gccgcctgct ggctcttcca caaacagctt ggccctcatt tggtagtgtg
      901 aaaatccgca atattctctg cactgtatta gaaattcgcc agcttgatct gggacatacc
      961 atatcgagtt tatcctgcct ggaatggcgt ctatcatcat agcaaagtct ggagtattaa
     1021 aagaatgatt cacgtctaaa gaagtcacct caaacctata gggagtgccc atcgtgaggt
     1081 gcagttctcc tagctctcgc cttccatctt catgctcaaa tgtccagaac cattgttgag
     1141 ctgtgacctt taccacttgg gcttcttcgg gagcatggtc tagaaatctt tcaacgttcc
     1201 atgcgtcggc tccaatgtaa atcagcacag cgactactac accgatatat acccattcta
     1261 atgtaccgtg tcccattagt ggtgtatacc tcctgctgat ggagtgatgt cttctcctct
     1321 attctttggg ttactctcac gaaatctcca tattacatag gtcatagttc ccatcacaac
     1381 tgccccgata acaaatgcta tgatcatgag tctgtagaat agcgcccaaa tctcaattct
     1441 tgcacttaaa tagcgctctg tagtctcctg tgcaaaggca gtagaatcga cgccgagaag
     1501 cgttagtgtc gctactggaa tcaagattaa aactatcgtt gaatatgaga ccatctattc
     1561 tctcactttt tcttattctg ataaaaacca gttttcttat cttatttaag gttttgcaaa
     1621 ttatctcctc attccgcggg gtagcggtgc atcaccataa tgttgactcc agaaattgag
     1681 tagatcctgc tttcgtgttt taatattgta agcgaagcat tgcgaatgtt ccatctgtga
     1741 agcgcctctg ggcttaagtc aagcaaagtg gcaagcgctg ccttgattgg atcaagatgg
     1801 gtgaccatca ggacattgct gtcttcgtgt ttctgcaata gatcgtcaag caggcttgta
     1861 atgcggtgct tgactgaggc aaacgcttcg actccaaagc tttcaagaac agggtcgtaa
     1921 tctgaataga acctcgtaag aacgtcgccg tactttgttg ttatctcctc gaagttcatc
     1981 ccaacaagcc ggcccatttc ggtttcgtat agtcggtcat caatcacata gtcagtacca
     2041 agtatttggc agactatctg agccgtttcc acagttctaa taacaggtga tacatagacc
     2101 ttttgaatag caatattttt catcagttgc tttgcgctat ccattacttg ctgtctgcc
//
LOCUS       DASQTF010000213         9471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_895466,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000213 DASQTF010000000
VERSION     DASQTF010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895466"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..599)
                     /locus_tag="VE308_05055"
     CDS             complement(42..599)
                     /locus_tag="VE308_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZB00371.1"
                     /translation="MATSTEQQITAEVEAPASVTIKKEGNLIQVKGTLGTVQKDFTKL
                     PATVTVEGNKITIKPYGTRKRDLAVTNTARSIIAGMIKGVEKGYTYKLKIIFAHFPIS
                     VKVRGKEVHVENFFGERSARMSRIIGDATKVSVVGEDVVIQGPSLEDVSQTAANIEQS
                     TKIKGKDQRVFLDGLYIYSKEEGTQ"
     gene            complement(583..975)
                     /locus_tag="VE308_05060"
     CDS             complement(583..975)
                     /locus_tag="VE308_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZB00372.1"
                     /translation="MPALNVLSNLFTTVYNNEARRKRECIVVPASKFASEVLRVMQKH
                     RYIGEFEQIDDGRAGKFRIQLLAKINKCGIVTPRFSVKKNGYFGWERQFLPAYTMGIL
                     LVSTSKGIMSHHEAQEQNLGGVLVGYVY"
     gene            complement(993..1172)
                     /locus_tag="VE308_05065"
     CDS             complement(993..1172)
                     /locus_tag="VE308_05065"
                     /inference="COORDINATES: protein motif:HMM:NF004424.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HZB00373.1"
                     /translation="MPKPREYEETGRKKLAHGKGGRWCKRCGSYNGLIRTYNLMLCRQ
                     CFREVANSIGFTKYE"
     gene            complement(1181..1711)
                     /locus_tag="VE308_05070"
     CDS             complement(1181..1711)
                     /locus_tag="VE308_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZB00374.1"
                     /translation="MSQQQVQQQHEMKKINVHKLVINIGVGRSGEPIDKAKNALLELT
                     SQKPAVRGAKNTVRDFGIHKGEPIGVIVTMRRGPAIEFLKRVIAAKRNVLKASSFDNY
                     GNISVGIHEHIDIPGTKYNPEIGIFGMDVNIVLNRPGYRIAKKSRKSAKIGKTHRINR
                     EEAIEFFKQEYGVEVE"
     gene            complement(1713..2426)
                     /locus_tag="VE308_05075"
     CDS             complement(1713..2426)
                     /locus_tag="VE308_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HZB00375.1"
                     /translation="MGKKGGDTRIKRQLAPTFWAIKRKEGQFVMRVNPGPHPGKRAYP
                     LGMVLRDVLHVATTGYEADRILKAGKVKVDGVIRRDSNFAIGLMDVVELANGQSFRMV
                     PKNSALLSPIAIEESEKGSKLIKVTSKTSIRGKKMQYGFHDSKTLISDQNLKVGDSCV
                     ISLPTVNITSHIPFEKGSTALIITGENAGAIGKIEDIQDGVFSLPKRALVSFGDKSVE
                     LPVEMVMAVGTDRPVIKVN"
     gene            complement(2426..2836)
                     /gene="rplX"
                     /locus_tag="VE308_05080"
     CDS             complement(2426..2836)
                     /gene="rplX"
                     /locus_tag="VE308_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZB00376.1"
                     /translation="MGGGINPKLIRLPKHQRDKMVGAVLEDSLRKQYGRKNIRVIKGD
                     SVRVMRGEYKGVEGKVEKLNMEHATFHIEGIQREKIRGGQVKVPIHSSNVMVISLNLD
                     DDYRSRKLQEATKSESASSEKEEAMEKKKEGDNE"
     gene            complement(2846..3286)
                     /locus_tag="VE308_05085"
     CDS             complement(2846..3286)
                     /locus_tag="VE308_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZB00377.1"
                     /translation="MAAGTKSRAVSAKGVEEFRPYITRAIPVTASLVCTDNTGAKILR
                     VAQVTRYKGRHSRQPAASVGDFVTVTVKKGPAELRKQIFGAVIVRQKYPIRRLNGVRV
                     AFEDNAAVLVTREGEIKGTDIKGPVASEAAEKWPRIANLAGMIV"
     gene            complement(3270..3611)
                     /locus_tag="VE308_05090"
     CDS             complement(3270..3611)
                     /locus_tag="VE308_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZB00378.1"
                     /translation="MVRNIGVSVASPRKTCEDEFCPFHGSLSVRGKLLAGTVSSARAP
                     KMVVVSREYPRAVAKYKRFQRSKSKVHAYLPSCLEVKEGEEVRIAECRRLAKTVSFVV
                     IEVNTKDGSRN"
     gene            complement(3898..4104)
                     /gene="rpmC"
                     /locus_tag="VE308_05095"
     CDS             complement(3898..4104)
                     /gene="rpmC"
                     /locus_tag="VE308_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZB00379.1"
                     /translation="MARLKVKTLREMNNQDLSDKLAELKTDLGKLQLEKSKGTLKKQT
                     GSIRWLRRDIARMKTIMNERREQK"
     gene            complement(4091..4801)
                     /locus_tag="VE308_05100"
     CDS             complement(4091..4801)
                     /locus_tag="VE308_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZB00380.1"
                     /translation="MSAVKNVIKNNFRNMELNEFLGEALKDAGYGGIEVQKTPVGARI
                     TVYVTRPGLAIGRKGVGIKDLMTKLEQRFGLQNPQISVLEIPNPELNPQIMCNRIAQL
                     IERGTAFRRAAMWSLNTIMSAGALGVEVVISGKLRTERAHFEKHTIGIVPKSGEVSDK
                     IVKTGITHVLTKMGLMGIQLRIASKVALPLEFEFKNGDTDMQASAAQPTSQTEESAKN
                     EEVTSGQEQKGGKKDGET"
     gene            complement(4798..5274)
                     /locus_tag="VE308_05105"
     CDS             complement(4798..5274)
                     /locus_tag="VE308_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZB00381.1"
                     /translation="MPQFGYAFQSYDKTKHVRASIREKDISHKHAREIAVAIKGRSIE
                     KARELLENVIARKEAIPYRRYNNEVAHRSNIRDGFFAGRYPKKAAYEFLRILDNLESN
                     AEYKGMDLDRLRIVSAAVHKGTKLKRFQPRAMGRSSPKFDTLVHVEVIAQEANERE"
     gene            complement(5280..5687)
                     /locus_tag="VE308_05110"
     CDS             complement(5280..5687)
                     /locus_tag="VE308_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZB00382.1"
                     /translation="MVREFKFKGYSPEQLQSLSIENLLPLLNSRQRRSLDKRVSSYMN
                     DDKRKLREKLKLARDGKLKGQLRTHTRDMIILPDMVGLTVYVHNGKDFAQVNIKAEMI
                     GHYLGEYAITNKRVQHGTPGVGASRSSLYVPLK"
     gene            complement(5751..6077)
                     /locus_tag="VE308_05115"
     CDS             complement(5751..6077)
                     /locus_tag="VE308_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZB00383.1"
                     /translation="MKPVTKAKKKQAATTSHKSNMTSAQAATLILAPYVTEKTFNQIE
                     KENKLAFIVTEKATKRQIIEAISMLYEADIEEVNTTRTIRGKKAFVKFKTPEGARDLA
                     TKLALV"
     gene            complement(6092..6904)
                     /gene="rplD"
                     /locus_tag="VE308_05120"
     CDS             complement(6092..6904)
                     /gene="rplD"
                     /locus_tag="VE308_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZB00384.1"
                     /translation="MKAKLIGLDGRDSGEIELPAVFDTPYRPEVIHKVYVNVLSHSYQ
                     RQGRYPAAGEMVSAESRNTGLGIARLARARGEGFPRAGQAAGVAGVRHGRVAHPPESW
                     KTIYKKINHKEKQLGLCSAIAVTAKKELVQRRGHKIGNEVKLPLVVSNDIESIAKTKD
                     LKSTLIRLGLGDDIARASLVRKARSGTPRRRGRSARSGISALIIVGNDSRLVALSRSI
                     PGVDIRHANDISILDLVPGSKPIRLTIFSQRAIEYMKNLQAPMHKVLEVIGK"
     gene            complement(6901..7899)
                     /locus_tag="VE308_05125"
     CDS             complement(6901..7899)
                     /locus_tag="VE308_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZB00385.1"
                     /translation="MGHRKYSAPRRGSIAFRPRARAKSLEARVRTWPQLAAEKSSLLG
                     FAGFKAGGIHILTVDDREKTPNFGKQLLNPATVIATPPIRIIGIRGYKHDLYGQHAIF
                     DVYAKDLPKELSRKFEVKRTDDGFSKKEPLLDTASDVMAIVAVSPNSIGLAQKVPFVF
                     EVAVSGKDSRSQYDYVKSILGKEIRITDIFQIGQNIDVFGITRGKGIEGPVTRFGVKR
                     KQHKSRKSVRAVGTLGPISPAVVMYTVARQGQRGFHQRTEYNKRILIISNTDRDAQSS
                     INPAGGFKHFGLVKGDYIVVRGSVPGVPKRLVKMRQPIRSISKRVLEPRVLEVVVK"
     gene            complement(8126..8281)
                     /locus_tag="VE308_05130"
     CDS             complement(8126..8281)
                     /locus_tag="VE308_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00386.1"
                     /translation="MSASSIGPTTDSGDKANPRARIAQPFVCQTCGKFFKSDNELNLH
                     KSLDHKP"
     gene            complement(8420..9280)
                     /locus_tag="VE308_05135"
     CDS             complement(8420..9280)
                     /locus_tag="VE308_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZB00387.1"
                     /translation="MPDLWIAIPNSSLSDEQTKRDKSIKIAQFARACSIFRVKRIYIY
                     HDSLSSFEKDDPHLLKTILRYLDTPQYLRKTLFPMMHQLQYAGILHPIKAPHHRVLED
                     IKKVKAGDVRTGVIVKIKGQLFVEVGLGSLVPFVGEGFEGKKVNVRFTSSYPNLKAVQ
                     ATEEDIFDYWGYEVKEVPSISKLLTSLEKTAIIITSRKGRYFKNIEPGLVQHFKKVQS
                     ILVAFGSPKHGVHDIIAREGASIKPYEYVVNMFPGQGTETVRLEEAVLGSLAILNSTL
                     QLTEHSGGPK"
     gene            complement(9282..>9471)
                     /locus_tag="VE308_05140"
     CDS             complement(9282..>9471)
                     /locus_tag="VE308_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HZB00388.1"
                     /translation="QVLIERVKQYLKSKEYSARTFDEYTLRGTPSVILVDRKGVLRGT
                     YFGSNGFLEGAVEELIQE"
BASE COUNT         2218 a         2347 c         1865 g         3041 t
ORIGIN      
        1 cactattaca gcaaatccaa ggacaaaata aatctttggg cttactgtgt accttcttct
       61 ttagagtata tgtataaccc gtcgaggaac actcgctggt ctttcccttt tatcttagtc
      121 gattgttcaa tgttagcagc cgtctgcgaa acatcctcta ggcttggccc ttgaatgacg
      181 acatcctctc caacgacgct caccttagta gcatctccta ttattcgaga catccttgcc
      241 gacctttcgc caaaaaagtt ctcaacatgt acctctttgc cccttacttt tacagaaata
      301 ggaaagtgcg caaatatgat cttaagctta tatgtatagc ctttttcgac accttttatc
      361 attcctgcga ttatgctgcg ggcggtgttt gtcacagcaa ggtcgcgctt tcgtgttcca
      421 tagggtttta ttgttatctt attgccttct accgtcactg tagccggaag ctttgtgaaa
      481 tccttctgca cggtgccaag tgtgcccttg acctgtatga ggttaccctc cttttttatt
      541 gtaacactag caggagcttc aacttctgct gttatttgtt gctcagtaga cgtagccaac
      601 caaaactcct cccaggttct gctcctgcgc ttcatggtga gacatgatcc ctttgctggt
      661 ggatacaagg aggatgccca ttgtgtaagc cggcaggaac tgtctttccc aaccaaagta
      721 tccgttcttt ttaaccgaaa agcgtggggt aacaatgccg cacttgttta tcttggctaa
      781 aagctgaatt ctgaacttgc cggctctacc gtcgtcaatc tgctcaaact ccccaatgta
      841 gcgatgtttt tgcataacgc gcagtacttc actagcaaac ttggatgcag ggactactat
      901 gcactctctc tttctcctag cctcattatt ataaacggtt gtaaagagat tagatagaac
      961 atttaatgct ggcatttttt tcttttcttc acctactcgt attttgtaaa tcctatgcta
     1021 ttggcaactt ctctaaagca ctgtctgcaa agcatcaagt tgtatgtgcg gatcagtcca
     1081 ttatatgaac cacacctttt acaccacctt ccgcccttgc catgcgcaag cttctttctt
     1141 cctgtttctt catactctct tggctttggc atttatttat ttattccacc tctactccat
     1201 actcctgctt aaagaactca attgcctctt ctctgtttat cctgtgcgtc ttgcctatct
     1261 tggcactctt tctgctcttc ttcgcaatcc tgtatccagg cctgttaagc acaatgttga
     1321 cgtccatgcc aaagataccg atctcaggat tgtactttgt accaggtatg tcaatatgct
     1381 catgtatacc aacagagata tttccataat tatcaaatga tgatgctttg agcacattcc
     1441 tcttcgctgc gatgacgcgt tttagaaatt caattgccgg cccgcgcctc atggtcacaa
     1501 ttacccctat gggctcacct ttgtgaatac cgaagtccct aacagtgttt ttcgctccac
     1561 gtacagctgg cttttgactt gtcagctcaa gcagagcatt ctttgctttg tctataggct
     1621 cgccggacct cccgactcca atgtttatga ccagcttatg gacgtttatc tttttcatct
     1681 cgtgttgttg ctgtacttgt tgctggctca acttaattca ccttaatcac aggtctgtca
     1741 gttccaacag ccattaccat ttcaactggc aattctacgg atttatcgcc aaatgaaaca
     1801 agtgctcgct ttggaagaga gaataccccg tcctgaatgt cttcgatctt tcctattgca
     1861 ccagcatttt cgcctgtgat gataagtgcc gtagaaccct tctcaaaagg aatatggctg
     1921 gtgatattga ccgtgggcag gctgataaca cagctgtcgc ctaccttcag attttggtcg
     1981 cttatgagtg tcttgctatc atggaagccg tattgcatct ttttgcctct aatactagtc
     2041 ttgcttgtaa ccttgatcag tttagatcct ttttctgact cctcaattgc aattggggac
     2101 aataatgctg aattctttgg aaccatcctg aatgattgac cattcgctag ttccactaca
     2161 tccattaatc caatagcgaa gttggaatcg cgtcttatca ctccgtctac tttgacctta
     2221 cccgccttca gaattctgtc tgcttcatat cctgtagttg caacgtggag cacatcccgt
     2281 aagaccatcc caagaggata ggccctcttc ccagggtgag gccctgggtt gactctcatg
     2341 acaaattgac cctcttttct cttaatggcc cagaatgtag gtgctagctg tctttttatt
     2401 ctagtgtccc cacctttctt tcccattatt cattatctcc ctcctttttc ttctccattg
     2461 cttcctcttt ttcggaagaa gctgactcac tcttagttgc ttcctggagc tttcgtgatc
     2521 tatagtcgtc gtcaagattt aaagaaataa ccatcacgtt tgaagaatga attgggacct
     2581 taacttgacc tccgcggatc ttttccctct ggataccttc aatgtgaaat gtcgcatgct
     2641 ccatgtttag cttttcgact ttgccctcta ctcccttgta ttctcctcgc attaccctaa
     2701 cagaatcacc cttgatcacg cgtatgttct ttcttccata ctgtttgcgc agtgagtctt
     2761 ctagtaccgc gccaaccatc ttgtcgcgct ggtgcttagg aagacgaatg agttttggat
     2821 taatacctcc tcccataatt ttcacctata ctatcatacc cgccaagttg gcaattcttg
     2881 gccacttttc tgccgcctct gacgctactg gtcccttaat gtctgttcct ttgatttctc
     2941 cttcccgagt caccagaact gcagcattgt cttcaaacgc tacacgcaca ccgttaagcc
     3001 tccttattgg gtacttctgc ctgacaatga ctgcaccaaa aatctgcttg cgcaactctg
     3061 ccggaccttt cttgaccgtc acagtaacga aatctccaac tgatgctgct ggctgccttg
     3121 agtgccttcc cttgtacctt gtcacctgag caacacgcaa tatctttgca ccagtgttgt
     3181 cggtgcatac taggcttgca gtaactggaa tagcccttgt aatataaggg cggaactctt
     3241 ctactccctt tgcagaaact gctctagact tagttcctgc tgccatcctt tgtgttcacc
     3301 tcaatcacta cgaatgaaac tgtcttagca agtcgtctgc actctgcaat ccgtacttcc
     3361 tcgccctctt tgacttcaag gcatgatgga aggtaggcat gtacttttga tttgcttctc
     3421 tgaaaacgtt tgtactttgc gactgctcta gggtattctc ttgatactac aaccatctta
     3481 ggcgcccttg cacttgatac ggtacctgcc aacaatttgc ctcttacaga cagtgaaccg
     3541 tggaatggac agaactcgtc ttcacaagtc ttccgagggg aagcaactga gacaccaatg
     3601 tttctaacca taatttatca tatcacccat tgtttaaccg tacaatacga tcctcaggtc
     3661 ttcctatcaa tgcggaacca cttataaagc aagcgcatga agatatttga agttggagtt
     3721 tttttgctgc cttttttgct acctgcttta caatggtatt cttcctaatg aatattgtat
     3781 tttttgtttc aaacacaatt gtgccggata tgccatgtaa agttgagtct gaagactcaa
     3841 gtatttttgc gcgaagaccc acaatcccat gccataggac atttccacag ttaactgtca
     3901 tttctgctct cttctctcat tcattattgt cttcatacga gcaatatctc gccggagcca
     3961 tcgaatgctg cctgtctgct tctttagggt gccctttgac ttctcaagct gaagctttcc
     4021 aagatcagtt ttcagttcag ccagcttgtc actcagatcc tggttgttca tctctcgtaa
     4081 tgtctttacc ttaagtctcg ccatctttct tgcctccttt ttgttcttgt cctgatgtta
     4141 cctcctcatt tttggcagat tcttcggttt gagaagttgg ctgagctgct gaagcttgca
     4201 tgtctgtatc tccattctta aattcaaatt ccagtggtag cgctaccttg gatgcaattc
     4261 taagctggat ccccattaag cccatcttgg ttagcacatg agtaatccct gtcttgacta
     4321 tcttatcaga aacctcgccg ctctttggta caatgcctat tgtatgtttt tcaaagtgag
     4381 cgcgctctgt tctaagcttg ccagaaatca caacctcaac ccccagcgcg ccagcgctca
     4441 ttattgtatt gagcgaccac atggctgccc gcctaaatgc ggtccctcgc tcaataagct
     4501 gtgcaattct gttgcacatt atctgcggat taagctcagg gtttggaatt tcaagaacgg
     4561 aaatctgagg gttttgcagg ccgaatcttt gctcaagttt ggtcatgagg tccttgatcc
     4621 caacgccttt cctgcctatg gcaagtccag gccttgtaac gtagacagtt atccttgccc
     4681 ccacaggcgt cttttgcact tcaataccgc catacccggc gtccttgagt gcttcaccga
     4741 gaaattcatt cagctccata tttcggaagt tattcttaat tacatttttg accgcactca
     4801 ttctctctcg tttgcctcct gtgcgataac ctctacatga accaaggtat caaactttgg
     4861 acttgatctg cccattgccc gaggctggaa ccgcttgagc tttgtgccct tgtgcaccgc
     4921 tgcactaacg attcgcagtc tatcaagatc catacccttg tattcagcat ttgattcaag
     4981 attgtctaaa attctgagga attcgtacgc tgcctttttg ggatatctgc cagcaaaaaa
     5041 tccgtcacga atattcgacc tgtgtgccac ctcgttattg tagcgccgat agggtatggc
     5101 ttctttgcgg gcaataacat tttcaagtaa ctcacgtgcc ttttcaattg acctgccttt
     5161 aatggccaca gctatctccc gagcatgctt atgagagatg tctttttcgc gtattgaagc
     5221 acgtacatgt tttgttttat catagctttg aaaagcataa ccaaactgtg gcatacttat
     5281 cacttaagtg gaacgtacag acttgatcta gacgcgccta cacctggtgt gccatgttga
     5341 acacgcttgt ttgtaatcgc atattcgcca aggtagtgtc ctatcatttc tgccttaatg
     5401 ttaacctgcg caaaatcctt gccgttatga acgtaaacag tcagaccgac catgtcagga
     5461 agtataatca tgtcgcgggt atgtgtcctc aattgaccct tgagctttcc gtctcttgcg
     5521 agcttgagct tctctcgaag ctttctcttg tcgtcattca tgtaagaact tacgcgtttg
     5581 tcgagcgatc tgcgctgtct cgaattgaga agcggcaaaa ggttctctat tgagagtgat
     5641 tgcaattgtt caggactgta acctttaaac ttaaactcac gtaccaatta gatcactcga
     5701 tctttggctt ctaggccact tttaaatcta acgagaaaaa aatagttcat ctatactaaa
     5761 gccaactttg ttgcaagatc tcttgctcct tctggggtct tgaatttgac aaaggctttc
     5821 ttgcctcgaa ttgtccttgt agtgttgacc tcctcgatat cagcttcata gagcatactg
     5881 atggcctcaa tgatttgcct cttggtagcc ttttctgtta caatgaaagc aagcttgttc
     5941 tctttttcta tctggttgaa tgtcttttct gtaacatatg gggcgagtat caaagttgct
     6001 gcttgcgctg aagtcatatt gctcttgtga ctagtcgtag ctgcttgttt cttttttgct
     6061 ttagttacag gtttcattgc tctatcctcg ctcactttcc gatgacctcc aataccttgt
     6121 gcataggtgc ctgtaggttt ttcatgtatt caattgcacg ttgagagaaa attgttagcc
     6181 ttattggctt tgagccaggc accagatcaa ggatacttat atcgttggca tgcctgatgt
     6241 caacgccggg tattgaacgg gaaagggcta caagtctaga gtcatttcca actataatga
     6301 gagcacttat tccagaacga gctgacctac ccctacgcct cggggttcct gaccttgcct
     6361 ttcgcaccag acttgcgcgt gcaatatcat ctccgagccc aagacgtatg agcgtgctct
     6421 tcaggtcttt agtttttgct attgattcaa tgtcatttga aacgactagg ggaagtttga
     6481 cctcgttgcc tatcttgtgg ccacgccgtt gtaccagctc ctttttagca gtaactgcta
     6541 ttgcagagca aagcccgagc tgtttttctt tatggttgat ttttttgtaa atggttttcc
     6601 aagactctgg agggtgggca actcgtccat gcctgacgcc agcaacaccc gcagcctggc
     6661 ctgccctagg gaagccttct cctcgggccc ttgcaaggcg ggcaatgcca agaccggtgt
     6721 tgcgagattc agcactgacc atctcgcctg ctgctggata gcgtccctgt ctttggtacg
     6781 aatgcgaaag gacatttacg tatactttat gaatcacttc aggtcgatat ggggtatcaa
     6841 aaactgcagg cagttctatc tcgccagagt ctctgccatc tagtccaatt agtttagctt
     6901 tcatttaaca actacctcca ggactcttgg ctcaagcacc ctcttcgaga tgctccgtat
     6961 tggctgtctc atctttacta accgttttgg aacgcctggg actgaacctc tcaccacaat
     7021 atagtcaccc ttgacaagcc caaaatgttt aaagcctcct gcaggattta ttgaactctg
     7081 tgcatccctg tccgtgtttg agattatcag tatacgcttg ttgtattctg ttctctgatg
     7141 gaatcctctc tggccctgcc tagcgacagt atacatgact accgcgggtg agataggacc
     7201 cagtgtacct acagcgcgca cactctttct tgatttgtgt tgttttctct taactccaaa
     7261 tctagtgacc ggaccttcta ttcctttgcc acgtgtaatc ccaaatacgt caatattctg
     7321 tccaatttga aaaatgtcag ttattcttat ttcctttccc agaatgcttt tcacatagtc
     7381 atattgcgac ctactatcct tgccagaaac tgctacttca aaaacgaagg gtactttttg
     7441 tgcaaggccg attgaatttg gagaaactgc aactatggcc atcacgtcac ttgcagtatc
     7501 gaggaggggt tctttctttg aaaatccatc atcagtacgc ttcacttcaa atttgcgcga
     7561 tagctctttt ggtaagtcct ttgcatagac gtcaaatata gcatgctgac cataaagatc
     7621 gtgcttatag ccgcgaatac cgataatcct gatcggcggc gtcgcaatta ctgttgcagg
     7681 attcaacaac tgcttgccaa aatttggagt cttctcccta tcgtcgactg ttaagatgtg
     7741 aatgccacca gctttgaaac ctgcaaaacc taaaagagag ctcttttctg ctgcaagctg
     7801 gggccaagtt ctcactctcg cctccagact cttagctctt gctcttggtc taaaagcaat
     7861 gctacccctt cttggggcac tatacttacg atgacccata gatactgcac cacactgcct
     7921 ctccctttaa taagttattt gacccccaag aatacggtaa tagtgaatat ctaaaaaaga
     7981 gaaaatcgat ggagtaaata ttcagaatat gtatattgag attgtcacat cgcggtggag
     8041 cgtgatgatt aatacatgac catgcaggga cacatatgcg cactgttatt gcagacaatg
     8101 agtgcgtcta tatgagacca tatctttacg gtttatgatc taaactcttg tgcaaattaa
     8161 gttcattgtc agacttgaag aatttgccac aggtttggca tacaaagggc tgggctatcc
     8221 ttgcccgggg gtttgctttg tcgccgctgt cggtagttgg accaatgctg gatgcagaca
     8281 ttttactaat aactcattta gggccggtat tggatttatc agttacaaat gaatttttag
     8341 attgaagata cgccttttat aattaatggg atcctcgctt caagacccat tggtctattg
     8401 tagtatttct ttgaagatcc tatttaggac cgcccgaatg ttcggttaac tgtaaagtgc
     8461 tattcaatat cgctagactt ccaagtacgg cttcttcgag tcttacagtt tcagtgcctt
     8521 gacctggaaa catgtttaca acatattcat agggcttgat actggcacct tccctagcaa
     8581 tgatgtcatg aactccgtgc ttgggcgatc caaatgcaac tagaatgctc tggacttttt
     8641 tgaagtgttg aacaagcccc ggctcaatgt tcttgaagta gcggcccttt ctcgatgtaa
     8701 tgattatggc ggttttttca aggcttgtaa gaagcttaga aattgaagga acctctttta
     8761 cttcatagcc ccaataatca aaaatgtctt cctctgttgc ttggacggcc ttgaggtttg
     8821 gatatgagga cgtgaacctg acattcactt tcttaccttc aaagccctca cccacaaaag
     8881 gcaccagaga tcctaggcct acttcaacaa aaagctgacc cttaatcttg acgataacgc
     8941 ctgtgcggac gtcgcctgct tttactttct tgatgtcttc gagtacccta tgatggggtg
     9001 cctttatcgg gtgtaatatg ccggcgtatt ggagctgatg catcatggga aatagcgttt
     9061 ttcggaggta ttgaggtgtg tcaagatagc gcaatattgt cttgagcaaa tgcggatcat
     9121 ctttttcgaa ggatgataga gaatcatgat agatatatat cctcttaact ctaaagatcg
     9181 agcaggctcg agcaaattgt gctattttta tgcttttgtc gcgctttgtc tgctcatctg
     9241 aaagtgacga atttggtatt gctatccaca gatcaggcat attactcctg tatcagctct
     9301 tcaactgcac cttccaaaaa gccatttgat ccaaagtaag ttcctctcaa aacacctttt
     9361 ctgtccacaa ggatgacgga tggggtgcca cgtaaagtat attcgtcaaa agtcctggcc
     9421 gaatactcct ttgattttag gtactgtttt acgcgctcaa ttaatacctg t
//
LOCUS       DASQTF010000214         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1109301,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000214 DASQTF010000000
VERSION     DASQTF010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109301"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..832
                     /locus_tag="VE308_05145"
     CDS             422..832
                     /locus_tag="VE308_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZB00389.1"
                     /translation="MSTTESLKVRHFIYKYFQLAQSKEIEKIEEFLDPRFTKFGDSPP
                     YDRRDFGRALMLEQLHFASLSDYDFKIENLNTEVMGDVAVATFLLEVTGMIVDDYSFR
                     GASVNNKSRVTIVLQKDKKDHWKMVHQHLSKLAG"
     gene            complement(829..1683)
                     /gene="lsrF"
                     /locus_tag="VE308_05150"
     CDS             complement(829..1683)
                     /gene="lsrF"
                     /locus_tag="VE308_05150"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HZB00390.1"
                     /translation="MDWGMQNRLAKIIHPEDGRCLMLAVDHGYFLGPMERLEEPRRAI
                     NPLLPYADSLMLTRGILRTSVDPKCNVPIVLRVSGATSIIGEDLSKETITTSIEEAIR
                     LNASCLALSIFVGSKYEHQTLANLAKLVDEGEKYEIPVLAVTAVGKEMGRDARYLGLA
                     CRVAAELGAHVVKTYYCDNFEQVVEGCPVPLIIAGGKKLPEPEALKLAYDAITHGAAG
                     LDMGRNIWQSDWPVAMIKAVQTIVHNNADAEEAYVIFLDEKENDKQSQKERQKVVRTT
                     VNQSMPFD"
     gene            complement(1727..>2568)
                     /locus_tag="VE308_05155"
     CDS             complement(1727..>2568)
                     /locus_tag="VE308_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZB00391.1"
                     /translation="AIGKSVKGFARGDRVFIHHHVACYSCHYCLHGDYTMCPAYQTSN
                     ISPCGLAEHFLVPEWNVSRGGLIKLPNNVSFDEASLVEPLACCIKAWKKCSFQKGDDV
                     VILGAGPTGLMHELLALAFGAGKIFVIDINDFRLNFAKKYGVQVFNPTSDHDFANNIK
                     LETGGRGADICIIATGNMKAVLQSFDLTRRGGKIMLFGVPPKGSQMSYDISKLYSSEH
                     SLIPSYAASEIETNQALKLIAEDRLDIGSLITHRFHISKAAEAVKCAYEANNAMKVII
                     TTD"
BASE COUNT          693 a          571 c          538 g          766 t
ORIGIN      
        1 aaattaagac taaattctaa tacatttgga gcttgaatga gatgcaagat gccagctatt
       61 gcagttgttg cagctgcagc ataatataga acgtttattt tctcaaccaa tgattaatga
      121 tcgacgtaga taggatatag agattatctc ttttttcaag gtactaacaa cagagagtag
      181 ggttcttgtt attgttgttg ctgttttaac tctggaaaga cccgtaacgc accatttgcg
      241 actataattc cttcttttga taaatagaac aacgtaccta aattgaaaat cttgacaaga
      301 gttcattgag gtagttccta aaatatttaa ttgtataaaa taagtataaa caacaggctt
      361 cagtggatca cagaatcgta aagctataaa aattttattc cctattattt aatgaacaga
      421 aatgtcaact acagaatccc ttaaggtaag gcacttcatt tacaaatatt ttcagcttgc
      481 ccagagcaag gagatagaaa agatagagga atttcttgat ccacgcttca cgaaatttgg
      541 cgattcacca ccctatgata gacgtgattt tggaagggca ctcatgcttg aacaattaca
      601 ttttgccagc ctctctgact atgatttcaa gattgaaaac ttgaatactg aggtgatggg
      661 cgatgttgca gttgctactt ttttgcttga agttaccggc atgatagtcg acgactatag
      721 ctttcgcggt gcgtctgtca acaacaagtc tagagtaacg attgtcttgc aaaaagacaa
      781 aaaggaccat tggaagatgg ttcaccagca tttatctaaa cttgccggct agtcaaaagg
      841 cattgattgg ttgactgtcg tcctaacaac cttctgcctt tctttctggc tttgcttgtc
      901 attctccttt tcatctaaaa agatgacgta tgcttcttcg gcgtcggcgt tattgtggac
      961 tattgtctgc accgccttta tcattgctac aggccagtct gactgccaga tatttcttcc
     1021 catatccaag ccggcagcac cgtgagttat tgcatcgtac gcaagcttca gtgcctctgg
     1081 ctcggggagt ttcttgccac ctgcaattat aagtggaacg gggcaccctt cgactacttg
     1141 ctcaaagttg tcacaatagt acgtcttcac tacgtgtgca ccaagttctg cagcaactct
     1201 gcatgcaagt ccaaggtaac gtgcatcacg ccccatctcc ttgccgactg cagtcactgc
     1261 aaggacaggt atttcatact tttcgccctc atctaccagc tttgctaaat tggcaagtgt
     1321 ctggtgctca tacttgctgc ctacaaatat tgatagggca aggcatgatg cattaagcct
     1381 gatcgcttct tcaattgaag tcgtaatcgt ttcttttgaa aggtcttcgc caatgatgct
     1441 ggttgcgcca gaaacacgaa gcactattgg aacattgcat ttcgggtcaa ctgatgtcct
     1501 caggatgcct cttgtaagca tgagcgagtc agcgtagggc aaaagtgggt ttatagctct
     1561 tcttggttcc tcaagacgct ccattggccc aagaaagtag ccgtgatcaa ctgcaagcat
     1621 aaggcaacgg ccgtcttcgg gatgaattat cttagctagt ctgttttgca ttccccagtc
     1681 catggtcgat cagctcagac atgtgatttt attatatata gtttcattaa tctgtggtta
     1741 tgattacctt catagcatta ttggcttcat aagcacattt taccgcctct gcagccttgc
     1801 taatgtgaaa cctatgagta ataagagatc ctatgtctag gcgatcttct gctatcagct
     1861 tcaacgcttg gtttgtttca atctcagatg ccgcatagct tgggatgagc gagtgctcgc
     1921 ttgagtagag tttacttatg tcgtaggaca tctgcgatcc ttttggcggg acgccaaaga
     1981 gcattatctt accgcctctt cgagtcaggt caaatgactg aagtactgct ttcatattcc
     2041 ctgttgcaat gatgcaaata tcggcacccc ttcctcctgt ttctaacttg atattatttg
     2101 caaagtcgtg atcagaagta gggttgaaca cctgcacgcc atatttcttg gcaaagttca
     2161 agcgaaaatc attgatatca atgacgaata tcttgcctgc cccaaatgct aaagccaaga
     2221 gctcgtgcat caatccggtt ggacctgcgc caagtatcac gacatcgtct cccttttgga
     2281 agctgcactt tttccatgct ttaatacagc atgcaagcgg ctcaacaagt gatgcttcgt
     2341 caaacgatac attgtttggt agttttatca ggccacctct tgacacgttc cactctggca
     2401 ctaagaaatg ctctgccaga ccgcacgggc tgatgttgct cgtctggtac gcagggcaca
     2461 tagtataatc accatgaagg cagtaatgac aagaatagca cgcgacatga tgatggataa
     2521 agacacggtc gccacgcgca aagcctttga cagatttgcc aattgcta
//
LOCUS       DASQTF010000215         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1006487,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000215 DASQTF010000000
VERSION     DASQTF010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006487"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(399..1709)
                     /gene="asnS"
                     /locus_tag="VE308_05160"
     CDS             complement(399..1709)
                     /gene="asnS"
                     /locus_tag="VE308_05160"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HZB00392.1"
                     /translation="MSVRTSEVKSSDYVGKEVMIRGWVYRLRKQKENAFVLVRDDRGG
                     VIQCVFPVEKTANLTIESSIEVTGIVSQDARAPEGGYEIKGKNLKIYNLAGTDYPIGE
                     YQSEELLLDKRHLAMRMRKMVAMAKIRATVLDYGRRWFVESGWMEVTAPIIVKGAVEG
                     GSTLFKLNYFDEEAYLSQSAQLYLEAMIFSLGPVWSLTPSFRAERSRTVRHLAEFSHL
                     EAEAPWVTLEDLLKVQEQLVSYVIQNTIRERAEELRYLGRDISELKKIEPPFERLSYE
                     EAIEIIKSKGLRITREDGSRKREIQFGDDLNIDSERELTKNVTKPIFVVGYPLAIKPF
                     YVKENPHRRDSGLAADLLAPRGFGEITSGGLREDNIVSITERIKKEGLNPAAYDWYLD
                     LRNYGSVPHGGFGLGIERLLRWITNAEDIKDTVPFPRTLSRVTP"
BASE COUNT          560 a          513 c          417 g          617 t
ORIGIN      
        1 tgcacgagtc ctacctagga taaaatatcc gtagcccatt atcaaggaaa ctggacgtct
       61 cagtacagtg tgttaagcca gtcaatgttt taagaataaa attaagccag acgctgccag
      121 ataatagaca gccataagtt caggattgca gatgacaaag gcaagtattt ttctatcttg
      181 cattaagatc cattgctcaa agtgtaaccc gacccgataa cggtaatacc ctaggtagta
      241 atgtcctgtt tctatagaat gtgaggcaag gccaagaata gaacagaaga atatgctgcc
      301 attccagttg aaaaacttct ttgatagttg cagctgaagg atcgactaca gagagccaga
      361 aaaaagaaat cagatgctac atccatgatt ttgatctatc agggtgtgac acgagatagg
      421 gtgcgtggaa aaggcacggt gtccttgata tcttcggcgt ttgttatcca ccgcagcaag
      481 cgctcaatgc caagcccaaa gccgccatgt ggtacagagc cataatttct taggtcaaga
      541 taccagtcat aggctgcagg attgagccct tctttcttga tgcgctcagt gattgaaaca
      601 atattgtctt cccttagacc accacttgtt atttcgccaa agccccgtgg cgctagcaga
      661 tctgctgcca gcccactgtc tcttcggtgt gggttttcct ttacataaaa gggcttgatt
      721 gcaagtgggt atcctaccac aaatatcggc ttggtcacat tcttagtcag ctcgcgttct
      781 gaatcaatat taagatcatc gccgaactgt atctctcgct ttctgctgcc atcttcccga
      841 gtaatcctta accccttaga cttgatgatt tcaattgcct cttcataaga tagtcgctca
      901 aacggtggct cgatcttttt cagttcagat atatcacgtc ctaagtatct cagttcctca
      961 gccctctccc tgatcgtgtt ctggatcaca tatgaaacaa gctgttcctg tactttcaag
     1021 aggtcttcaa gagtgaccca tggcgcctcc gcctcgaggt gggaaaactc tgccagatgc
     1081 cggacagttc gagatctttc tgcacgaaac gaaggcgtga ggctccatac tggtccgagt
     1141 gaaaaaatca tggcttccaa atacaactgc gcgctctgcg acaggtatgc ttcttcatca
     1201 aaatagttta gcttgaaaag tgtggaaccc ccttccactg cacctttcac aatgattggc
     1261 gcagtcacct ccatccagcc gctttccaca aaccaacgcc ggccatagtc aagtacagtt
     1321 gcccttattt tcgccatcgc gaccatcttg cgcatgcgca ttgcaagatg ccgcttgtca
     1381 agcaacaatt cttcactctg atactcacct atagggtaat cagtgcctgc aagattgtat
     1441 atcttgagat tctttccctt tatctcatat cctccctctg gagcccttgc atcttggctt
     1501 acaatgcctg tcacctcaat tgaagattct atagtaaggt tggctgtctt ttcgacaggg
     1561 aagacgcatt ggatcacacc ccccctatcg tcgcgtacaa gcacaaatgc attctctttt
     1621 tgcttcctta accggtatac ccagcccctg atcatgactt ccttgccgac atagtctgac
     1681 gacttgacct cacttgtcct gacggacatt gaggtttagg tttcttatct aattcttaag
     1741 tttatccagc ttaaaccttg aactattgac ctctgtaggt cagaacatcg agaactccct
     1801 taggatattc ctattagaga ttttgctttt ggcaacaata cttttatctg tactactttg
     1861 gcatgggtgg gattgaatga acccacgacc ttagctagct taaggctgct ctaattgttc
     1921 aactcttaaa acctgtcccg atagttgctc atagtagcag agcacaattc gaataagcag
     1981 tcatgataca ttcttattaa ttactgtaca ttatataatg cctagaagaa attgatatga
     2041 catgcatctg aactaacaat tttcagcacg gtttttattt gatataagtc taaaattttt
     2101 gtttatg
//
LOCUS       DASQTF010000216         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1007758,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000216 DASQTF010000000
VERSION     DASQTF010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007758"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VE308_05165"
     CDS             <1..402
                     /locus_tag="VE308_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZB00393.1"
                     /translation="IQKIEKMVTDKSTLPFEVEYSQKPSSKSMTPTSMKRLAIVLKPE
                     NVDSITSTLRGLGLESTIYDVKGSGKEKERVSTGRGVSTGDLSYTTRRVVATVIKSDT
                     VEDIMDRMKKALGDGSGAVVMIQPVDELVRL"
     gene            909..1280
                     /locus_tag="VE308_05170"
     CDS             909..1280
                     /locus_tag="VE308_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZB00394.1"
                     /translation="MTFGKVGAVILLVSDMETSTRFYRDVLNLPIKSQSKDWTEFFNR
                     EIVLALHPARKAPNQSTSPQGNVLVGFMVSDLEAEVQQLQKKNVKFFKRLKEKPFGKH
                     AIMEDPDGHLISLAEIKSKGG"
     gene            complement(1551..2318)
                     /locus_tag="VE308_05175"
     CDS             complement(1551..2318)
                     /locus_tag="VE308_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00395.1"
                     /translation="MTYLNNKSNKNNHPVEAAAATAATTISILAIAIIIIIIMITSTT
                     IASPVAATTTTTASAGTTTTTSSAAAAPSSGIKLSPQPIYQESTPPGSVTPINQTHIA
                     ATHTGNGTLTIPNSNQTINVISNGTAIASLATPSAIAKTTIRAPNGETATITIYEIIQ
                     FSNPAEAPLGGGKGFKIFIFHTNSTGMLAPLDGVIAAGINDIQPNGESELTAWRWESG
                     IPLSPQANSTSGANATDPLSVPSGGAAAAGGTAELQR"
     gene            complement(2445..>2801)
                     /locus_tag="VE308_05180"
     CDS             complement(2445..>2801)
                     /locus_tag="VE308_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00396.1"
                     /translation="TLPNTTETINTTSNGSSLTSLATQSAIGTEIIMTEAGETIIATF
                     YEILEFPPGAGGANGISIAVLHTNSTGRLAPLNGTIVVGTDYLYPISEGFSTYWEWVP
                     TPPMYATTRAAAPPMQ"
BASE COUNT          795 a          508 c          666 g          832 t
ORIGIN      
        1 attcaaaaaa ttgaaaaaat ggttacagat aaatcgactt tgccctttga agtagagtac
       61 tcccaaaaac caagcagtaa atcaatgact cctacttcca tgaagagatt ggcaatagtc
      121 ctaaagcctg aaaatgtaga ttcaattacg tctaccctta gaggattagg gcttgaatca
      181 acaatatatg atgtgaaagg ctcaggaaag gaaaaggaga gagtctcaac aggcagagga
      241 gtaagtacag gcgacctttc atatactaca agaagagttg tcgcaacagt aatcaaatca
      301 gatactgttg aagatatcat ggatagaatg aaaaaagctc ttggtgatgg aagtggagca
      361 gtagtcatga tacagcctgt agacgagtta gtgcggctgt aactatggct gaaggagaag
      421 gaagaagacg acattttcac aattgcaaag tcataagcag gtagacagta taaggaagaa
      481 aatctccgaa tctaacatcc cacagttaca tccgacctga catatgtaag agtcagagga
      541 tgctgatgag gaaaaggaga gaatatactt atgacatgca agaatatctt ggtcaatttt
      601 aagactgtca aaaatagtaa ctcgaagtcc gtatctcgaa ggagagaaat gatgtgcagg
      661 ttgctgcatt tttatagagt gggaggggat acattgccct tgttgtacac gcgagcttcg
      721 ggtcaaccct agaagcaaga aaagttggtg ctatgcgacg ttaaggagca tccgacttgg
      781 caaagaggac agtaggctgg tagaagcaag taaacagaca ccatcgccat caatttaata
      841 cagcaataaa caatgaatgg gagaaaaaat attagtgatt agggagtaac ttagatgctg
      901 cctaagatat gacgtttggc aaggttggag ctgttatact actggtatca gacatggaaa
      961 cctccacaag attttacaga gatgttttga atttgccgat aaaaagccag tctaaagatt
     1021 ggactgaatt cttcaacaga gaaatagtcc tagcacttca tccagctcgc aaagcaccga
     1081 atcaatcaac aagtccacag ggaaatgtgc ttgttggttt tatggtcagt gaccttgaag
     1141 ccgaggttca gcaactacag aagaaaaatg tcaagttctt caaaaggctt aaggagaaac
     1201 cttttggcaa gcacgcaata atggaagacc ccgatggtca cctaatttca ttggcagaaa
     1261 taaaatcaaa agggggatga aggttttgac cttatcggtt tttttggtgc agagtagccc
     1321 atagccagac ctcaatgtct gatgcaacag cgatagcacc tactactgaa ctagctcgcc
     1381 ccaattcata aagtgtgctt ttttctcgat agtttgactc ttgcgttatt ttcttccaca
     1441 ctagggtggg agagtttggc ggtcacctta accgcaaatt gcgcaaccaa taaaccgccg
     1501 tcctctgttc tttatgtgcg atcatctttt ttcttatggc ttaaattttc tcatctctgt
     1561 aactcggcgg tgcctcctgc tgctgctgct cctccgctag ggacagaaag ggggtcagta
     1621 gcattagcgc ctgaagtact atttgcttgt ggtgataatg gtattccact ttcccatctc
     1681 catgctgtta gttcgctctc accatttggc tgtatgtcgt taattcctgc cgctatcaca
     1741 ccgtctaatg gtgccagcat accagttgaa ttggtatgga agataaatat tttaaatcct
     1801 ttgcctccac ctagaggagc ttctgctgga ttgctgaact gaattatttc ataaattgtg
     1861 atagttgcag tctcaccatt tggtgctctg atggttgttt ttgcaatagc agatggcgtt
     1921 gcaagtgagg caatggctgt accgttgctt atgacattaa tagtctggtt gctgttagga
     1981 atagttaatg tgccatttcc agtgtgtgtt gcggctatgt gtgtctggtt aattggagtc
     2041 acgcttccag gtggtgtgct ttcttggtag ataggttgcg gtgataactt tattcctgat
     2101 gatggtgctg ctgctgctga agatgtagta gtagtagttc ctgctgatgc tgtagttgtt
     2161 gtagttgctg ctactggaga agctatggtt gttgatgtaa tcattattat tattattatt
     2221 attgctattg caaggataga gatagtagta gctgctgttg ctgctgctgc ttctacagga
     2281 tggttgtttt tgttgctttt gttatttagg tatgtcatat gtcatttaca agcatgaatg
     2341 cttaggctaa atgtattctc gcttgataga tattttgaaa tataatgata agttttgctc
     2401 tttagcttta gggtcaactg tttatgatta gtatagtgcg gttgttactg cataggagga
     2461 gcagcagctc tggtggtggc atacattggt ggtgtcggta cccactccca atatgttgaa
     2521 aaaccctcac taattggata caggtaatct gttcctacta ctatcgtgcc attcaatggt
     2581 gcaagtctgc ctgttgagtt tgtatgaagc actgctattg aaatgccgtt ggctccaccc
     2641 gccccaggag gaaattcaag aatctcataa aatgtggcga ttatagtctc gccagcttca
     2701 gtcataatga tctctgtccc tatagcagat tgtgttgcaa gtgaggtaag tgagctaccg
     2761 ttgctagtgg tattgatagt ctctgtagtg tttggaagag t
//
LOCUS       DASQTF010000217         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1267183,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000217 DASQTF010000000
VERSION     DASQTF010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267183"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VE308_05185"
     CDS             complement(<1..832)
                     /locus_tag="VE308_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZB00397.1"
                     /translation="MSTETQGFGVSKREGHNSSRFYSRKLYRQNTTNNGESNGDGKYG
                     ENPIEPQNLNKLFCKSSEKMDELPDYSVHLMVTSPPYNVGKVYDQDLSIKEYRSLLKQ
                     VFKETFRVLVHGGRACINIANLGRKPYIPLHSYIIEDMHELGFLMRGEIIWNKAASSG
                     VSTAWGSWQSASNPTLRDVHEYVLIFSKGTFSRSSHNKENTITKDEFLTFTKSTWDFP
                     AESATRVGHPAPFPIELPYRCLQLYTFKGEVVLDPFCGIGSTCIAAIKSGRQFVGYDV
                     N"
     gene            complement(967..1113)
                     /locus_tag="VE308_05190"
     tRNA            complement(join(967..981,1012..1032,1076..1113))
                     /locus_tag="VE308_05190"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Trp,seq:cca)
     gene            complement(1165..>2083)
                     /locus_tag="VE308_05195"
     CDS             complement(1165..>2083)
                     /locus_tag="VE308_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00398.1"
                     /translation="AATTFIPEGWDADLQIIRPYKSMTGFVFFARGDENTLVYVFQPF
                     MPLYILPSTSLCESDSICFSSIISAEKVREMSLGNAPISVSNFKTPEQYFTSEVLPML
                     TRNLNAYTVDSAESVYASIYGGGNSTDQVPAYDVDYNFNLKNKKISGKAMVFTRNYTT
                     TAGGTGIWNGFIVGIESSENNFDKAFQQASVTLLNLQFEEPWLNSERNVLIENANTSQ
                     VLGAIPELMANSTLDDFYLIVPTAAHQLVTSYNNTVVADYFDNDSGKELHLPLFPELQ
                     YWYLNNGDQLVGRDLRRDLMNASSLEPLF"
BASE COUNT          574 a          453 c          421 g          635 t
ORIGIN      
        1 tgtttacatc atatcctaca aactgtcttc cactttttat ggcggcaata catgtagaac
       61 ctataccgca gaagggatca agcacaactt cacctttgaa tgtataaagt tgtaggcaac
      121 ggtatggaag ctcaattgga aaaggagctg gatgaccaac acgagttgca gattctgcag
      181 gaaaatccca ggtgctcttt gtaaatgtaa gaaattcgtc ttttgttata gtattctcct
      241 tgttatgtga gctgcgagaa aaagttccct tagagaagat tagcacatat tcatggacat
      301 ctctcaatgt cggattagat gcagattgcc aacttcccca tgcagtcgaa actccagagc
      361 ttgcagcctt gttccaaatt atctcgccac gcataagaaa gcctaactca tgcatatctt
      421 cgattatgta gctatgtaat ggaatgtatg gttttcttcc taaattcgca atattgatgc
      481 atgctctacc accatgcacg agtacacgaa aagtctcttt aaaaacttgc ttcagcaagc
      541 ttcgatattc ttttatgctc aaatcttgat cataaacctt gccaacatta tatggagggg
      601 aggtaaccat aagatgaaca ctataatcag gtagctcgtc catcttttcg ctgcttttgc
      661 agaacagttt gtttaagttt tgaggctcaa taggattctc tccatattta ccatcaccat
      721 tactttctcc attatttgtt gtgttctgtc tgtaaagttt acggctatag aatcttgaag
      781 aattatgacc ttccctcttt gaaactccga agccttgtgt ttctgttgac atccgttttc
      841 tcgatcggct cacagttgat agagcttgat atttatagat aagaattttg tcaaagtaat
      901 ctattccatc taaaaaatgc aattcggcgc acaaaaagag agcattgccc attagggtca
      961 aattagtggg gccgaaggga tctgtcctga cgcggctgtt gccacaatca attgaaccct
     1021 tgacccacgg gtttcttctt ctttttctcc tgataattta gcccttttgt tagatctgga
     1081 gcccgttgcg atgcctggct tcgctacgac cccactaaat agcatcgtgt tatggataga
     1141 ataaagatgt ctgtataagt cttgctagaa taggggctca agcgagcttg cattcatcag
     1201 atcccttctg agatctcggc cgacaagctg atcaccatta ttaaggtacc aatactgcaa
     1261 ctcaggaaat agcggaagat gcaattcctt gccgctatca ttatcgaagt agtctgctac
     1321 cacagtattg ttataagacg ttaccagctg gtgcgccgct gtcggaacta taaggtaaaa
     1381 atcatctagt gtactatttg ccatcaattc cggaattgca ccaagtacct gacttgtatt
     1441 tgcattttca atcagtacat tcctttcgct attaagccac ggttcttcaa actgtaaatt
     1501 caatagagtc actgacgcct gctggaatgc tttgtcaaaa ttattttcgg aggactcgat
     1561 tccgacgatg aagccgttcc aaatgccggt gccacccgca gtggtggtgt aattacgcgt
     1621 aaataccatc gcctttccgg aaattttctt atttttgaga ttaaaattgt aatctacatc
     1681 gtaggctggc acctgatcag tggaattgcc accgccatat atcgacgcat aaactgactc
     1741 tgccgagtca acagtgtatg cattcaggtt tcttgtcagc attggcaaaa cttcacttgt
     1801 gaaatactgc tctggcgttt taaagttaga aacagatatt ggggcgtttc caagggacat
     1861 ttcccggacc ttctctgctg atattataga actgaagcaa atgctatcgc tttcgcaaag
     1921 cgacgtgctt ggaagaatgt ataaaggcat gaacggctgg aagacatata caagcgtgtt
     1981 ttcatctcct cttgcaaaga atacaaagcc agtcatagat ttgtatggcc tgattatctg
     2041 gagatctgcg tcccaaccct cagggatgaa tgtagttgcg gct
//
LOCUS       DASQTF010000218         6004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_266718,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000218 DASQTF010000000
VERSION     DASQTF010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266718"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..544
                     /locus_tag="VE308_05200"
     CDS             338..544
                     /locus_tag="VE308_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00399.1"
                     /translation="MGRVDKGINCSVDSCGNQAERSMSGIKAGMAPDLGLGGSKRVYL
                     CRDHYKEWKKLTKEDRENERARWA"
     gene            577..2463
                     /locus_tag="VE308_05205"
     CDS             577..2463
                     /locus_tag="VE308_05205"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZB00400.1"
                     /translation="MRLLQLHSDFIEYEPIEKEIRDAEEIASKSKIRLEDLVVAFVAV
                     ENRDDESVANMAVDEIKKYLDTVKSSRLLIYPYAHLSSDLAPPNFAIDIIKSVESFAK
                     GSINQVYRAPFGWTKSFNIKVKGHPLAENSKEISKQEEHEHADPLHREKESSALKNEE
                     KLQSLWYIAQPGGQMTPVSKYNFKKQDQNLQMLVDYELAKNRAVNEQPAHVRLMKKLA
                     IADYEPASDAGNIRYYPKGRLMKSLIEQYITRRVMEYGGIEVETPIMYDTKHPSLQSY
                     FNRFPARQYNITSDNKQLFLRFAACFGQFLMAKDFNISYKHLPLKLYELTRYSFRREK
                     SGELVGLRRLRAFTMPDCHAFCRDLDQAKKEFLKRFELSISVLNGFGLTIKDVEMAVR
                     FTEDFYNENKDFMTELINRFGKPVLVETWKERFFYFTLKWEFNFIDGLAKASALSTDQ
                     IDVENGARYNVEFVEEDGTKKNPIILHNSPSGAVERVIYALLEKSAKISKGGGIASLP
                     LWLSHTQVRIIPVSQEHIIYCEKVLAELTENQIRTDIDDRDETVGKKIRESETEWVRY
                     TIVIGDKEISSDKLIVRDRNEGKQREITLHHLINEVKAQTKDKPYLPLNLPLRLSARP
                     QIIV"
     gene            complement(2453..3391)
                     /locus_tag="VE308_05210"
     CDS             complement(2453..3391)
                     /locus_tag="VE308_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZB00401.1"
                     /translation="MSELDILSLFIVPERTKAGYGFADIPVKISFWESNVVIYGVPLD
                     ITTSFGKGTSRGPEAIRRTSALQSETFLLDEKTNIHDLVGIFDLGDLKLLKTRSIIDM
                     LDHLDSTIPKVVASLRNDSKIPVMLGGEHTLSYYQLKALAKEEPIVIHFDAHRDMKPN
                     YESLKICHTTPFYHLLKYIPGENLIQIGIRQTDKEEDETASKNGVTTFDAWEVHNDID
                     TVVSYLQKATAGKKIYISFDIDAYDLPYVPCTGTPEPFGLDPFQVLKLVKAIDLSACL
                     IGMDMVEVALKNGDYREGTLATQTLYRILSRKYARQ"
     gene            complement(3419..>6004)
                     /locus_tag="VE308_05215"
     CDS             complement(3419..>6004)
                     /locus_tag="VE308_05215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase I"
                     /protein_id="HZB00402.1"
                     /translation="VYSGEEKKVYLKFYDPQDKIIYQWRDRTDHKPYCYTKMEFAEKA
                     EQVAKKETKFILKQVKKQDIILDKEIEVLKVIAPDPLSIGGTDYSFREKVTSWEADIK
                     YHENYLYDRALIPGSYYVRNDEQITPFEYPISEKVHFALRSLLWDKIKETGEAGKEYR
                     QYITNWAKLLNQPIPELKRVAIDIEVETEEGRMPNPRDHDRRITAVGFVGSDGFKKVL
                     VLVNNISSDGTPLVPEAEPCATEKELIEKAFMIINEYPIILTFNGDDFDLPYLYARSL
                     DSRIDPNGIAITKDKVPIMVKKESFIKHGMQAEPVGIRHGIHIDLYRTFDNRSIQIYA
                     FSRAYTDHTLNAISEALLGDSKIEFEGDISDLPIQRLAEYCLKDADLTYRLTSFNDNL
                     LMKLLVAVARIGRLSVDDIARFGVNQWIRGILYYEHRQRNELIPSRSELEKKGTSSTT
                     AIIKEKKYRGGEVVEPVPGIHFNVIVLDFASLYPSIIKVHNLSYETINCSHEDCRENE
                     VEGTTHWVCKHRKGLTSFLIGSLRDLRVNYYKNLAKDKTLPAEERKLYNVVSQAIKVI
                     LNASYGVMGAAIFPLYCLPVADATAALGRTTINRTINKCKEIGIRVVYGDTDSIFLSN
                     PDQKHIEQLVQWARETLGVDLEIDKQYRYVVFSDLKKNYLGVLSDGNVDVKGLTGKKS
                     HTPPFIRKAFYDIMEILGKINSEKDFESAKENIKNVIQENAKNLEAHKIPLEELSFNV
                     TINKSLSTYGKKTGSKTVDGNSSDIYKGLPQHIKAAKMLVDANKREVRAGDIISYVKI
                     KAGDGVRPVELSKPEEIDTEKYLETMESTFEQVVSALNFDFKSVLGKPRQKSLDELFW
                     SKSAS"
BASE COUNT         1570 a         1360 c         1382 g         1692 t
ORIGIN      
        1 tcctcaaagt tattgttgtc gtcttcggac atgcctgact gagatcaatc cactgaacgc
       61 ttgttataaa ttttgccagt tagtgttctt gggctcttta cttgtccgcc ttttgcttga
      121 cggtcaccat gcaagagcaa atggccacgg tgcaccgctt tatctgtccc acatcctggg
      181 gcaaacttgc cactatgtgt gtgtgaagct cctgagggtg aagacattta gtacagagag
      241 gatcattgta tggcaactgc cgtaattata tgctgcctga ggtattaagc taatccagtc
      301 agctttttat agccaggaaa taatctcatg caatcatatg ggccgagtag acaagggcat
      361 caactgcagc gtcgatagct gtgggaacca ggccgagcgc tcgatgagcg ggattaaggc
      421 cggcatggcc ccagatctag gattaggcgg tagcaagagg gtctatctct gcagggatca
      481 ttataaagaa tggaaaaaac ttaccaaaga agacagggaa aacgaaaggg ccagatgggc
      541 ttaaattaga tataagatta ggattaggat taagatatgc gactgctgca gctccattca
      601 gattttattg aatatgagcc tattgaaaag gagatcaggg acgccgaaga gatcgcgtcc
      661 aaatctaaga tcaggctcga agatctggtg gtagcttttg tagcagttga aaacagagac
      721 gatgaaagcg ttgccaatat ggcagtcgat gagataaaga aatatcttga tacggtcaag
      781 agcagtcgcc tgctaattta cccttacgct catctgagct cggaccttgc gccgccaaac
      841 tttgcaattg atatcatcaa atctgttgaa agcttcgcca agggatcgat aaaccaggtc
      901 taccgcgcgc cttttgggtg gacaaagtcg tttaacatca aggtaaaggg ccacccacta
      961 gctgagaatt ccaaggagat ttcaaagcaa gaagagcatg aacacgctga cccattacat
     1021 agagaaaagg aatcatcggc actcaagaat gaggaaaaat tacagtcgtt gtggtacatt
     1081 gcgcagccag gcggacagat gactcctgtc agcaaataca acttcaaaaa gcaggaccag
     1141 aatcttcaga tgctggtaga ctatgaatta gcaaagaaca gggcagtcaa cgaacagcct
     1201 gcacacgttc ggctaatgaa gaaattagcg atagccgact acgaacctgc gtcagacgcg
     1261 ggtaatattc gctattatcc aaaaggcagg ctgatgaagt cgcttatcga gcaatatatc
     1321 acaaggcggg taatggagta tggtggaatt gaggtcgaaa cacccataat gtatgacacc
     1381 aaacatcctt cgcttcagag ttacttcaac aggttccctg caaggcagta taacattact
     1441 tcagacaaca aacagctgtt tctgcgcttt gctgcctgct tcggccagtt cctcatggcc
     1501 aaggatttca atatatcata caagcatctg cccctaaaat tatatgaatt gacaagatac
     1561 tcatttaggc gcgaaaaaag tggtgagcta gtaggcctgc gccgattgcg agcgtttact
     1621 atgcctgact gtcacgcatt ttgcagagac ttggatcagg caaagaagga gtttctcaag
     1681 cgctttgagt tgtcaataag tgtgctcaac ggttttggcc taacaatcaa agatgtggaa
     1741 atggccgtaa gattcacaga ggacttttac aacgagaaca aagacttcat gaccgagctt
     1801 atcaacaggt tcggcaaacc ggtgctagtt gaaacatgga aggagcgctt cttttacttc
     1861 acacttaagt gggagttcaa tttcatcgac ggccttgcca aggcatcggc tctctcaacc
     1921 gaccaaatag atgttgagaa tggtgcgcgc tataacgtgg agttcgttga agaagacggc
     1981 acaaaaaaga acccgataat tctgcacaac tcaccttcag gcgcagtcga gcgggtaatc
     2041 tatgcgctat tggaaaaatc agccaagata tccaaaggag ggggtatcgc ctcgctaccg
     2101 ctctggctgt cacacacgca ggttcgtata ataccagtca gtcaagagca tatcatatac
     2161 tgcgaaaagg tacttgcaga gctgactgaa aaccagataa ggaccgacat cgatgaccgc
     2221 gatgaaacag taggcaaaaa gatacgtgaa tctgaaaccg aatgggtgcg ctacaccata
     2281 gtcattggcg acaaagagat cagctctgac aagctgatcg tacgcgacag aaacgagggt
     2341 aaacagcgcg agattactct gcatcatctg ataaatgaag tcaaggcgca aacaaaggac
     2401 aagccttatc ttccgctgaa ccttccgctg cgcctatcag ctaggccgca gattattgtc
     2461 tagcatattt tcgcgataat attctgtaca atgtctgagt agccagtgtg ccttcgcggt
     2521 agtccccatt tttcaaggcg acctcgacca tgtccattcc aatcaggcag gcagatagat
     2581 ctattgcctt gaccaatttc aatacctgaa atgggtcgag gccaaatggt tccggcgtgc
     2641 cagtgcaggg tacgtatggc agatcgtagg catcaatgtc aaatgaaatg tagatttttt
     2701 tacctgcagt cgccttttgc aaatagctta cgacggtgtc aatatcattg tgcacctccc
     2761 atgcatcaaa ggttgtgaca ccgtttttgc tagcggtttc gtcttcttct ttgtctgtct
     2821 gtcgaatacc tatttggatc aggttttcgc cggggatata tttcagcaaa tgatagaagg
     2881 gagtggtatg gcagatcttc agactttcat agttgggctt catatcgcgg tgagcatcaa
     2941 aatgtatcac tattggttct tctttggcaa gcgccttcaa ttgatagtat gacagcgtat
     3001 gctcgccgcc gagcattact gggatcttgc tgtcgttgcg gagtgaagca actacctttg
     3061 ggattgttga atcaagatgg tcaagcatgt cgatgatgct cctagtcttg agcagcttta
     3121 ggtccccaag gtcaaatatg ccgacaagat catgaatgtt ggtcttttca tccagcagga
     3181 atgtctcgct ctggagagca gaagtccgcc ttatcgcctc cggtcccctc gatgtccctt
     3241 tgccaaagct ggtggtgatg tcaagaggta cgccataaat aactacgttt gattcccaga
     3301 atgaaatttt gactggaatg tccgcaaagc cataacctgc ctttgtcctt tccggcacta
     3361 taaagagcga aagtatgtcc aactctgaca caaatcttca aaaccggttg ataataaatt
     3421 atgatgctga cttgctccaa aacagctcgt caagactctt ttgtcgcggc ttgcctagta
     3481 ctgacttgaa gtcaaagttt aaggcagata ccacttgctc aaatgtcgac tccattgttt
     3541 caagatattt ttcagtgtca atttcttccg gctttgaaag ctcgactggc ctcacgccgt
     3601 cgcctgcctt tattttcaca taagaaatga tgtcaccggc tcgaacctcg cgtttgtttg
     3661 cgtccacaag catcttggca gccttgatat gttgtggcag gcctttgtag atgtcactac
     3721 tgttgccgtc caccgtcttg gagccagtct ttttgccata tgtactcaat gatttattga
     3781 tggtgacgtt gaagctcagt tcttcaagtg ggatcttgtg tgcttccaag ttcttggcat
     3841 tttcttggat aacgttctta atgttttctt tagcggattc aaaatctttc tccgagttga
     3901 tcttgccaag tatttccatg atatcataga atgccttcct gataaatggc ggagtatgtg
     3961 actttttgcc tgtaagccct tttacatcaa catttccgtc agataagact ccaaggtagt
     4021 tctttttgag gtcgctgaac acaacatagc ggtattgctt atctatttca aggtctacgc
     4081 ccagcgtctc tctggcccac tgcaccagtt gctctatgtg cttttggtcc ggattgctga
     4141 ggaaaatgct gtcagtgtca ccataaacaa cccgaatccc aatctctttg catttgttga
     4201 tggttctgtt gatggttgtc ctgccaaggg cagcagtagc gtctgctacc ggcaggcagt
     4261 agagagggaa tattgctgcg cccatgacgc cataactggc attgagaatg accttgatag
     4321 cctggcttac cacgttgtac agcttgcgct cctctgcggg aagtgtcttg tcctttgcca
     4381 ggttcttgta atagttgacc ctcaggtcac gaagcgatcc tataagaaaa gaagtcaaac
     4441 cttttctgtg cttgcagacc cagtgggttg taccttctac ttcattttcc ctacagtctt
     4501 catgagagca attgatggtt tcgtatgata aattatgcac tttgataatg ctaggataga
     4561 ggctcgcaaa gtctagtaca atgacgttaa aatgtatgcc tggtacaggc tcgacgactt
     4621 cgccacctcg atattttttt tctttaatga tggctgtagt cgacgaggta cctttttttt
     4681 ccagctcgct cctactgggt atcagctcat ttcgctggcg atgctcgtag tacaaaatgc
     4741 ccctgatcca ttgattgacg ccaaagcggg cgatatcatc tacagacagc cggccaattc
     4801 ttgcaacggc gactaatagt ttcattagca gattgtcatt aaagctggtc agccggtatg
     4861 tcaggtcggc gtctttgagg cagtattctg ctaatctctg aatgggcaaa tcgcttatgt
     4921 caccctcaaa ctctattttt gagtcgccca acagtgcttc gctgatcgcg ttcaaggtat
     4981 gatcagtgta ggcacggctg aaagcataaa tctgaatcga cctgttgtca aatgttctgt
     5041 agaggtctat gtggatgcca tgtctgatgc caaccggctc tgcctgcatc ccatgtttga
     5101 taaatgactc ttttttgacc attatgggca ccttgtcctt tgtgatcgca atgccattag
     5161 gatcaatgcg agaatcaagc gagcgcgcat agaggtaagg caggtcaaag tcgtcaccat
     5221 tgaacgttag tatgatcgga tactcgttga ttatcatgaa tgctttttct atcaactctt
     5281 tttcagttgc gcagggctct gcttcaggaa ctagcggtgt gccatcactt gatatgttat
     5341 ttacaagcac gagcactttc ttgaaaccgt cacttccaac aaaccctaca gctgttatac
     5401 gcctatcatg gtcacgtgga tttggcattc ttccctcttc agtctcaacc tcaatgtcga
     5461 tagccactcg cttcagctct ggtattggct gattgagcag tttggcccag tttgtgatgt
     5521 attgtctata ttccttgcct gcctcccctg tttcctttat cttgtcccac agtagactcc
     5581 tgagtgcaaa atgcaccttc tcagaaatag ggtactcaaa tggtgttatc tgctcatcat
     5641 ttctaacata atatgaacct ggtattagcg cgcgatcata gagatagttc tcatggtatt
     5701 tgatatcggc ttcccatgac gttacttttt cacgaaagct atagtcggta ccacctattg
     5761 agagcggatc aggtgcaatc actttgagaa cttcaatttc cttgtcaaga atgatatctt
     5821 gtttcttgac ctgtttgagt atgaatttgg tctctttttt cgcaacctgt tcggccttct
     5881 ccgcaaactc catctttgta tagcagtagg gtttgtggtc agtcctgtcg cgccattggt
     5941 agataatttt gtcttgcgga tcgtaaaact tcagatagac tttcttctct tcgccagagt
     6001 aaac
//
LOCUS       DASQTF010000219         5867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1191552,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000219 DASQTF010000000
VERSION     DASQTF010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191552"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VE308_05220"
     CDS             complement(<1..358)
                     /locus_tag="VE308_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZB00403.1"
                     /translation="MTIHTVSSNMIPINKPWIGEEEKREVTSVLEETALTSAARDGGK
                     RVRDFESLMKSYLKIRHVVSVNSGTAAIYAALLAAGIKQGFENKIDLVYIDPPFLSGE
                     RYLHRVKNNSNLAFEDV"
     gene            571..1677
                     /locus_tag="VE308_05225"
     CDS             571..1677
                     /locus_tag="VE308_05225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZB00404.1"
                     /translation="MNNNRQYNLKVMVMAQRTKLICKQNNYGGVRNRNLLFHCDNLEC
                     INYLLKQGFENKIDLVYIDPPFLSGERYLHRVKNNSNLAFEDVLKESEYLDMMQERLS
                     GIRKLISSSGSIFIHLDWHAIHYVKVMMDQIFGSENFRNEIIVKRGRRKNLQYQFNSI
                     DRMHAAHDTILWYSKLADTKFRPPLAEYHSKAKWMGFWSNVDRPTMRYEIFGSKPVRG
                     QWKWAKERALKAIENYQVYENNFSHMILEEYWQDTSKKLEFVRKLSHRKYPEYWIPPK
                     THRIIDNVWLDIEAYNYSTGYGTEKHTQLLERIISQFSKPDDLVADFFCGSGTTLTVA
                     EKLRRHWIGCDSSLAAIAVTKKRLAGNFITVDIH"
     gene            1705..2139
                     /locus_tag="VE308_05230"
     CDS             1705..2139
                     /locus_tag="VE308_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HZB00405.1"
                     /translation="MKAQSASDYEVMLKRLQDKVGNTAKKARSRLEIPTPEIIWVGKN
                     TIFRNFMDFPKALRRDPEKFLLYLNKELASAGYIAGERVIFLGRKTLSSFQALIDRYI
                     KDYVQCPVCGSPDTHTEKIKPKVAFLICEACGAKSSIRGNYA"
     gene            complement(2607..3542)
                     /gene="pyrB"
                     /locus_tag="VE308_05235"
     CDS             complement(2607..3542)
                     /gene="pyrB"
                     /locus_tag="VE308_05235"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HZB00406.1"
                     /translation="MPNPFYHRDIVSIKDFAKDDLEFVFDATDSISRLKHSQRGELGK
                     GRTLGYIFYEPSTRTRMSFEAAMASLGGSSIGISDLKSSSVEKGESLADTIRVIDLYS
                     DVILLRHPMDGSSRFAAELSQNPIINAGSGSEEHPTQALLDLYTILKEKGKIDGLSVA
                     IIGDLKYGRTVYSLLYGLANYKVEVHLVSPPTLRIRKESLYEIQGKLKIKEHSKLDEV
                     LPETDVIYVTRIQRERFPDVQEYEKVKGTYTIDEHTLAKSKSDIAVMHPLPRVDEISQ
                     SIDHTKNAIYFKQASYGKELRAALLALMLNENLPR"
     gene            4059..4724
                     /locus_tag="VE308_05240"
     CDS             4059..4724
                     /locus_tag="VE308_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HZB00407.1"
                     /translation="MAIYRYTVYKSPNFGIFVKANDSIILVPYGFAETKTEKLADYLQ
                     VSEVYASIAGTRLLGPMTVMNNNGMLLPPIASDEEVQMLKQSSHLNVERVKSKFTAIG
                     NLIATNDNGAIVSPLFKGEIDRQVQDILGVPTNSMTVGGFVQTGSMIVATNGGAGVHP
                     KATEDEIKEISEILQVPAEPVTVNGGVPFLSSGIVANMKSVVVGNLTSGPELIMLSRI
                     FKA"
     gene            4721..5518
                     /locus_tag="VE308_05245"
     CDS             4721..5518
                     /locus_tag="VE308_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZB00408.1"
                     /translation="MIVEILCIGTELLSGITLNTNAYWLCSEITNVGGVMRRVTVVRD
                     DLIEISSAVRESLARKPNILITTGGLGATYDDMTLEGLAVALGKKVELDNRAVEMLKK
                     SYARRKLHYELTESRLKMATIPEGSTPIQNPVGSAPAVMEQAGGISIFCLPGVPSEMK
                     AIFEEHILPLIKKSVGKFVAEELNYNTKGLTEAMIAPVLIKIVKSHPKNMIYLKTHPR
                     GYYRKKTPQIKIQMISRGDDEKEVKNRLVAISKVIEKEIVKRGGIIC"
BASE COUNT         1691 a         1227 c         1296 g         1653 t
ORIGIN      
        1 atacatcttc aaaggctagg ttgctattgt ttttaacgcg atgtaaataa cgctcaccgc
       61 tgagaaaggg cggatcgata taaacgagat ctattttgtt ttcaaagccc tgctttatgc
      121 cagcggcaag tagcgcagca tatattgcag cagtgccaga attgacagac actacatgtc
      181 tgattttcag gtagcttttc attagcgact caaagtcacg cactcgtttg ccgccgtcac
      241 gcgcagctga agtgagtgcg gtttcttcaa gcacacttgt tacctcacgc ttttcttctt
      301 cgcctatcca gggcttgtta attggaatca tatttgacga tactgtatgt atggtcaata
      361 tcttatcata tagtattttt gcttataatg agtctcatta tagaaatgag ggttataatg
      421 gttaaagaag tattagaact gcaactaaag acatagaaga aaatatgcgt atattatatg
      481 tagctacatc gaccttgcat caatcgctat cttggatttg ggctttgaag aagccagaaa
      541 cgtccaggtt ggagtgtcta gttatgcgat ttgaataata ataggcagta caacctcaag
      601 gtcatggtca tggcacagag gactaagctc atctgcaagc agaataatta tggtggtgta
      661 aggaacagga acctgctttt tcactgtgat aaccttgaat gcatcaatta tttgctaaag
      721 cagggctttg aaaacaaaat agatctcgtt tatatcgatc cgccctttct cagcggtgag
      781 cgttatttac atcgcgttaa aaacaatagc aacctagcct ttgaagatgt attgaaagaa
      841 agcgagtatc ttgacatgat gcaagagcgc ttgagcggga tccgcaaact gatctcatct
      901 tctggatcca ttttcattca cttggattgg catgccatac attatgtcaa ggtgatgatg
      961 gatcagatct ttggctccga aaatttcaga aatgaaataa tagtaaagcg tggccgtagg
     1021 aagaatctgc aatatcaatt taattcaata gataggatgc atgctgccca cgatactata
     1081 ctgtggtatt ccaagttggc tgataccaaa tttcggcctc cgcttgcaga gtaccattca
     1141 aaggcaaagt ggatgggttt ttggagcaat gtagacaggc ctacaatgcg ctatgagatc
     1201 tttggctcca agcctgtaag gggacaatgg aaatgggcaa aagagagggc actaaaagca
     1261 attgaaaatt atcaggtata cgagaacaat ttctctcaca tgattcttga agaatactgg
     1321 caagatacta gcaaaaaact agagtttgtc agaaagctgt cacatagaaa ataccccgag
     1381 tattggattc ctccaaagac acacaggata attgataatg tttggcttga tattgaagcg
     1441 tataactatt ctacgggcta cggcacggaa aagcacaccc aacttctgga acgcataatc
     1501 agccagtttt caaaacctga tgatcttgtt gctgactttt tctgcggatc aggcactaca
     1561 cttacggttg cagaaaagct taggcgtcac tggatcgggt gtgattcatc gctggcagca
     1621 atagcagtta cgaaaaagag gctggcaggt aattttatca cagttgatat acattaatta
     1681 aacagaagtg caaccgatct atctgtgaaa gcgcagtctg ctagcgacta tgaagtcatg
     1741 ctcaagaggc tgcaggacaa agtgggcaat actgccaaga aggcaaggtc aaggctcgag
     1801 attccgactc ccgagataat ctgggtcggt aagaatacta ttttccgcaa ctttatggac
     1861 tttccgaagg cactaaggag ggatcctgaa aaattcctat tgtatcttaa caaagagctt
     1921 gcctctgctg gttacatagc tggcgagcgc gtgatatttt tgggccgtaa gacactctcc
     1981 tcatttcagg cgctcatcga ccgctacatc aaagactatg ttcagtgccc agtgtgtggg
     2041 agccccgaca cccatactga aaagatcaag ccaaaggtcg catttttaat ctgcgaggct
     2101 tgtggagcca agtcatcgat aaggggcaac tatgcctagt tctagatctg acctgtcgac
     2161 aagaagttgt aattgtaaaa aagtctttat ccaagcctga acaacaacaa caaacgtgtc
     2221 attttggtat gataacttga actatgcatt catctgatga aacttaactg cctttttcct
     2281 gactacatag ctcctgctta cgacatgctt gtcaatgttc gaagaactta taataatctc
     2341 atcgcaaatg ttgcatatta tttccatgca tcttgaatta tgatcgtatt atgtattgca
     2401 ataccgaatt agcttgagga tatatacatt cttgaaatga ggtattttct atcctgaaga
     2461 gacatttctt atgaaatgtc caataagcag aaatctgtca agagtaaact tcgacagatt
     2521 ctttgttttt atgcttccat aatagagata aaagataatt tgcagtagtt acttattcct
     2581 tgtcatgcaa gttagttttt gtcaagctac cttggcaggt tctcattaag cataagagcc
     2641 aatagtgcag cccttagctc ctttccgtaa gatgcctgct tgaaatatat tgcattttta
     2701 gtatgatcaa ttgactgtga aatttcgtcc acccttggca aggggtgcat gactgcaatg
     2761 tctgacttag actttgccaa tgtatgttca tctattgtat aagtcccctt gaccttttca
     2821 tattcttgca catccgggaa gcgctcacgc tgtatccttg taacgtaaat gacgtcggtc
     2881 tctggcaaga cttcgtcaag cttgctgtgc tcctttatct tgagcttgcc ttgtatttca
     2941 tagagcgact ctttccgtat ccgcagtgta ggcggcgaca caagatgtac ctccacctta
     3001 taattggcga ggccatagag cagtgaatat acagttcttc catattttag atcgcctatt
     3061 atagcaaccg acagcccatc gatcttaccc ttctccttca atatagtata caagtcaagc
     3121 agggcctgcg tcggatgctc ttcgcttcca ctcccagcgt ttatgatagg gttttgtgac
     3181 agctcggctg caaacctact agagccatcc ataggatgac ggagtaggat gacatctgaa
     3241 tagagatcaa taactcgtat tgtatcagca aggctctcac ccttttctac agaagacgac
     3301 ttcaaatcag agatgccgat cgagcttcct ccaagcgacg ccatcgcggc ttcaaagctc
     3361 attctcgttc ttgtggaagg ctcatagaag atatagccga gcgtccttcc cttgccaagc
     3421 tcacctcgct ggctatgctt gaggcggctt atactatcag tagcatcgaa cacaaattcc
     3481 aaatcgtctt tggcaaaatc ctttatcgaa acaatgtctc tgtggtaaaa gggattaggc
     3541 atggctacta ccttaccaca agcagactat aataaacact atgaccttga acttccttga
     3601 tttttcatac tcaaggattg ctgcttttct tgcttgatcc atccagcact ttgttgtatc
     3661 gaagtacaag gtgatcgcgc ttttgatgag ctttttatac caacctttgc tgctccttga
     3721 atagtgtata ttatatgata tgatattcca cctcctcttc ttctttgacg gatcgcagtc
     3781 atggattaag atcactggtt tattactaga atataaaaaa ctagaataaa gaaggtatgt
     3841 ttgtagtgac agatgacaga ttgtatattg aagacatagt taacgaatta gtaggcaagt
     3901 ctggatggtc gctactacag gagcatttaa aaattatctt gaaaccggaa ccttggaagc
     3961 tagttttttg gtataatgat ctcagaatct catcagatag aacgatcaat tgctagcgct
     4021 aggttcatat cctttatttt ccgagttttc tatacatatt ggcgatatat cgctatacgg
     4081 tatacaagag ccccaatttc ggcatatttg tcaaggcaaa tgacagcatc atcttagtac
     4141 cctatggttt tgccgagaca aagacagaaa agcttgccga ctacctgcag gtaagcgagg
     4201 tgtatgcttc catagcaggc acccggcttc taggccctat gacggtcatg aacaataatg
     4261 gcatgctgtt gcccccaata gcatctgatg aggaagtcca gatgctgaag cagtcaagtc
     4321 acctaaatgt tgagcgggtg aaaagcaagt tcacggctat aggcaatcta attgcaacaa
     4381 acgataatgg cgcaatagta tcaccgctct ttaaaggaga gatcgaccgt caggtgcagg
     4441 atatactcgg tgtcccaacc aactctatga ctgtaggggg ctttgttcag acgggatcaa
     4501 tgatcgtcgc cacgaatggt ggtgcaggtg tccaccccaa ggctacggag gatgagatta
     4561 aggaaatttc agaaatattg caagtaccgg cagagccggt aacagtgaat ggcggcgtgc
     4621 cctttctgtc atcaggtatc gtagcgaaca tgaagtcagt cgttgtaggc aaccttacaa
     4681 gcgggcctga gctaattatg ttgagcagga tcttcaaagc atgatcgttg aaatcctctg
     4741 cataggcacc gagcttctat caggcatcac tctcaacacc aacgcatact ggctttgcag
     4801 cgagattaca aatgtcgggg gtgttatgag aagagtgacg gttgtacgtg acgatcttat
     4861 tgaaatttcg tctgcagtaa gagaatcgct tgcaagaaag cccaatatct tgataactac
     4921 aggaggcctt ggtgcgacct atgatgatat gactctcgaa ggcctggcgg tagcacttgg
     4981 taaaaaagtt gagctagata atagagccgt cgaaatgctc aagaaaagct atgcaaggcg
     5041 caagcttcac tatgagttga ctgagtcgcg gctaaagatg gccaccatac cggagggctc
     5101 caccccaatc cagaatcctg tagggagtgc acctgcagtc atggagcagg caggtggtat
     5161 atcgatcttt tgtctgcctg gagtaccgtc ggagatgaaa gcaatattcg aagaacacat
     5221 cctgccactg ataaagaaga gcgtaggtaa gtttgttgct gaggagctca actataatac
     5281 aaaaggcctc actgaggcca tgatcgctcc agtgctgata aaaatagtca aatctcatcc
     5341 caagaacatg atctatctca agacgcatcc acgcggctac tacaggaaaa agacgccgca
     5401 gataaagata cagatgatat cacgcggtga cgatgaaaaa gaagtgaaaa ataggcttgt
     5461 cgcgatatca aaagttattg aaaaagaaat tgtaaaacga ggcggcataa tatgctgaca
     5521 tttttgattg tggggatagt agggggagcc gttagcgcgt tctatcttgc ctacaactac
     5581 catccactgc tgataaatgg tgtgatacat ggtggaattt ttgtttcacc acttgtcgcc
     5641 atgtattttg ttaagaaatg gaatgtgcgt actgttggct tgtatctagc tagcgcatta
     5701 cctgtgcatc ttgcaataca ttttataata ataggcgacc ttgctcagtt gattggtgta
     5761 agccacatct tgctaaattt tctatcgctg ccgtgaacta ctgcatcgaa cagcaacctt
     5821 agttgctttg tcctcttgcc tgtctggccg tcgcctatcg ttcgatt
//
LOCUS       DASQTF010000220         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1192124,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000220 DASQTF010000000
VERSION     DASQTF010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192124"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..218
                     /locus_tag="VE308_05250"
     tRNA            join(84..116,148..154,174..218)
                     /locus_tag="VE308_05250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:151..153,aa:Leu,seq:cag)
     gene            529..831
                     /locus_tag="VE308_05255"
     CDS             529..831
                     /locus_tag="VE308_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00409.1"
                     /translation="MIITLRDDLDAVSAILNETFKIPSFKRSYDEAFGKIAQILYRYG
                     IVENARNLRFRFILCMQEFFEGIPQDQWHVKDPAFVKQSEDFAISKFREWIIEQVT"
     gene            complement(824..1063)
                     /locus_tag="VE308_05260"
     CDS             complement(824..1063)
                     /locus_tag="VE308_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00410.1"
                     /translation="MIEAEDSIRVLDTIAKNLMGVSNNRSNFERLPDTFWGYYARGHN
                     GKGRFGVIVVYSENGNDVDELIKMYEHWVAKNKTR"
     gene            complement(1172..2209)
                     /locus_tag="VE308_05265"
     CDS             complement(1172..2209)
                     /locus_tag="VE308_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZB00411.1"
                     /translation="MEIIEKLKNLSIIGSIVVMPDFFVDRIIRLESKENLFDTLTEKA
                     KVGGGSVRGIPTTDVKGGNAVNVAYCLAKLGAKVSLFTVADEFGATMIRQTFSHFGDK
                     VTLNITSGRSGLTTSFEFPYEDTRVNVMVSDIGDNENFGSERISSEADRTILKNADGV
                     MLVNWASNLKGTELAEFSFKNSPSAFHFLGPADIEARKHDFRDSLAKLASLTDCLSIN
                     ENECNSLADALGFGRLLGPSYSADEVKGAAKRIAERVGISTDLHTNIGAAWSNGKESA
                     FAHAIKVEVKRLTGAGDTWDAAAIVGYLVGLHPQERLLFANCCASLYVRDPNGEPPSM
                     NKVFELVERVL"
     gene            complement(2280..2690)
                     /locus_tag="VE308_05270"
     CDS             complement(2280..2690)
                     /locus_tag="VE308_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00412.1"
                     /translation="MDFAVKIGASKERTITVNSNQTTSFLWKGENVLSTPSMIAEMEE
                     TCRLLLKEQVIPDSEWDSVGTVVKIEHLAPTPVGAEVFFKAEVVSVDDRRVVFKLDAR
                     DKLEKIGEGTHERFIINIPRFRVKFNEKQKKLQM"
BASE COUNT          954 a          868 c          739 g         1078 t
ORIGIN      
        1 agagcggcgg cggcgtgcca atcaacatcg aataagaaca acaatattat tctagtgagg
       61 cagaaatagc acaacgaatc taggcggggg tcgccaagcc cggtcaacgg cgtgagatca
      121 tcatctatat tctgatgatg acgcgatgct cagatgttgt aattgactgg gacacctcat
      181 cccttagggg ttcgtgggtt caaatcccac cccccgcact attttattag taatatttta
      241 tttttagaat actactactc tgattgtgtg tgttataata tatccaaaaa ggctcttttg
      301 ctgttgcttt gcttgccaaa aagagaggca tttgaagaaa catgagatag tgagcattgg
      361 tcttgtattc attatgcatt tcaatagtta aatgtgctct cgtagtgcaa gtatcgatga
      421 caatgacgat gacgacgaca gaaaatttgc aacacttcag aattgaagta acagcataaa
      481 agccttttat acgccaaaaa gtgggacaca tcttgaaaac aagaacatat gataattacg
      541 ctacgcgatg accttgatgc agtttctgca atactgaatg aaacctttaa gataccttca
      601 ttcaagcgaa gctacgatga agcctttggc aaaatagccc agatcttgta tcgctacggc
      661 attgtcgaaa atgctcgcaa tctgcgcttt cgctttattt tatgcatgca ggagttcttt
      721 gagggtatcc cgcaggatca gtggcacgtc aaagaccctg catttgtcaa gcagtcagag
      781 gattttgcga tatcgaaatt ccgcgagtgg ataatcgaac aggtcaccta gttttgttct
      841 tggcaaccca gtgctcatac atcttgatca attcgtccac gtcgttgcca ttctcagaat
      901 acacaacaat aactccgaat ctgcccttgc cattgtgacc tcttgcatag tacccccaga
      961 aggtgtcagg cagtcgctca aagttggacc tgttgtttga tactcccatt aggtttttgg
     1021 ctatggtgtc taatactctt atggaatctt cagcttctat catcaacatc tactggcttg
     1081 atcgccaatt taaaaaaatc cccgcagaag ttcaaatatt atattacact caacaaagga
     1141 accttttgat gcgcaggaaa tggcataatc actacaatac cctttctacc agctcaaaga
     1201 ccttgttcat tgagggtggt tctccgtttg gatcccttac atagagcgag gcacaacagt
     1261 tggcgaacag cagtcgctct tgtgggtgca gacctacaag gtaaccgaca atagcggccg
     1321 catcccatgt gtctcctgca ccagtcagtc tttttacctc gaccttaatt gcgtgtgcaa
     1381 atgcactttc tttcccgttt gaccatgctg ctcctatatt tgtatggagg tcagtgctga
     1441 tgcccactct ttcggcgatt cttttggccg cccccttaac ttcatcagca ctataactgg
     1501 gtccaagtag gcggccaaag ccaagtgcat cggcaagtga gttgcactcg ttttcgttta
     1561 tactgagaca gtctgtcaag ctagcaagct tggcaagcga gtcgcgaaaa tcatgctttc
     1621 ttgcctcaat gtcagcaggg ccaagaaaat ggaaggcgct aggggaattc ttgaaagaga
     1681 attctgccag ctctgttccc ttgaggttac tggcccagtt gactagcatc actccatcag
     1741 cgttcttcaa tatcgtcctg tctgcttctg agcttattct ttcggaacca aaattttcat
     1801 tgtctcctat gtcgctcacc atcacgttta cacgtgtgtc ttcataggga aactcaaaag
     1861 atgtggtcag gccgcttctg ccgctagtta tattgagtgt aaccttatca ccaaaatgtg
     1921 aaaatgtctg ccttatcatt gtagctccga actcatcagc aaccgtaaaa agtgatactt
     1981 tggcgccaag ctttgcaagg cagtacgcta cattaacagc gtttccaccc ttcacatctg
     2041 ttgtggggat gcccctgacg cttccacctc caacctttgc cttttccgta agcgtgtcaa
     2101 aaagattctc ctttgactcg agccggatta tgcggtcaac gaaaaagtca ggcatcacta
     2161 ctatagagcc tatgatgctc aggtttttta acttctcaat tatttccaca gatgctcaag
     2221 atgattaaag gcgtatatga tactttatgg gattgagatg aggagaattt taaccatttc
     2281 tacatttgaa gctttttttg cttctcgttg aatttcacac gaaagcgagg aatatttatt
     2341 atgaatcttt catgcgttcc ctcacctatt ttctcaagct tatctcgagc gtctagcttg
     2401 aacacgacac gcctgtcatc caccgatacc acctcagcct tgaagaatac ctctgctcca
     2461 acaggggtag gagcaagatg ttctattttg actactgtgc caaccgagtc ccattcagag
     2521 tcgggtatca cctgctcctt gaggagaagc ctgcatgtct cttccatttc agctatcatg
     2581 gagggtgtgg aaagaacgtt ttcgcctttc cagagaaagc tagtagtctg gtttgagttg
     2641 actgttatag tgcgttcttt tgatgcgcct atcttgaccg caaaatccat gcctaaacga
     2701 gatcacaagt tattttttaa acttgtgcat gaggatgtcc atcttttttc ttctaattct
     2761 tctcttaggt tttgtgatga tgtgagtcta tccttctctc ctgcaggaat ttcctccaca
     2821 cacatacatc ccgtatttta catatatctt cttaaaaagc aagaaaaagt catgttccaa
     2881 agagagtgta agttatcaaa aatgatgata tagtgacata gctggatgcg tgtaagtggc
     2941 agcaaaggaa gccttatccc gccagcaaag gacatactac tatctagtag aactcgcggc
     3001 ctgtactacc actactttta tggctgctgc atctgaaata tgtcccacaa taataatagg
     3061 atgacaccgc caacaaagaa gaccgcataa acacaacaag gatgccaact gctttctcat
     3121 gcaccaagtc tggtggttat taccgctatt gttgttttca tctttgttac agcaataaca
     3181 gaatagtgtc ctcagagtgt aggtgtagct attaacagaa gtgagtagga gagcaacaac
     3241 tgctgccaga cctgctgcca tagaatcact gctttgctgc ttatcatctt tgactaagcc
     3301 cctcttctgg ctcttatgtc aattcttctt ttagtgttgt tgcgcatgtt atcgattcct
     3361 cttgtgttga tgttgtggtg ttttactagc agcatttgac ctttcattag gcgtctgctg
     3421 tcaatcacag gtcgaccgtt tggatcttca tcacatgcct gacggtgata aagaagtctg
     3481 cgcttgcacg tgtatcagca gccaacatca ccggggtccc ttccaggagg aactacgcaa
     3541 tcatcaaatt catcatcgcc tgctccgcct ccaccgtctc caccatcatc gtctccattt
     3601 ccaccatcat cgtctccatt tccaccatca tcgtctcca
//
LOCUS       DASQTF010000221         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1011865,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000221 DASQTF010000000
VERSION     DASQTF010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011865"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..1760)
                     /locus_tag="VE308_05275"
     CDS             complement(294..1760)
                     /locus_tag="VE308_05275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZB00413.1"
                     /translation="MTSLASVNVNRHAWFTLGILSSTLLVVFFSETMLLPAIPEIMED
                     FSISYGTAAWIFSAYLIVAAVMTPIAGKMSDLYGKKKILLVLLTIYTAGIIAGGFADN
                     ISFLLASRIIQGVGLAAVPAAFSLLRDTFPPAKLAIAVGVFGSMYSAGSVVGLLAGAT
                     IIQSFGWHFTFFSIVPFAATVTIMIAKFVKENKGQLLAPVNTQESTGKKQASPSIDLK
                     GALALSTTIIAFLIALTLLETGINSETLPQIGAAFIVSVISLSVFVIIERKIASPLVD
                     LRLLKDKTLLPSYIILMASGITMFMAYPAIVQLVRSPVPLGFGGDAVDAANVQLPFMI
                     MFLVFAAVTPLIINRIGKLRPIIIGAMISLIGSIGLLMFHSTEFAVSTDLAIIASGLS
                     MTITATWNIIVSSSPKSFIGISVGVGALLLFIGMAIGPALTGVYLESHETVKGVPGSY
                     SSLESYDVVYFTSALLSAASLGFALVLKRRVAKKEIDV"
     gene            1923..>2255
                     /locus_tag="VE308_05280"
     CDS             1923..>2255
                     /locus_tag="VE308_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZB00414.1"
                     /translation="MSTDSCTCGELRKAARAVTLLYDNAFKSSGLLSTQLDLLYVIYK
                     SDSIRISHLAKELGMDRTTLTRNLSVLERQGFIKISSGKDNRTRIVTITYKGRSTVAK
                     TIPLWNEVQ"
BASE COUNT          652 a          474 c          482 g          647 t
ORIGIN      
        1 aatgctcttc tctccttttc tctttgattt ttagaatata cagattgaac ggctccaggt
       61 ctatatgcca atttagcata attggactaa ttgacataat ccgatattgt tatgcagtta
      121 atgtcttctg ttggtgactg atgagtggtt tgatattttt tcatgtagag acattaatga
      181 ctcctcatgg aatattaaat tgcatctata acatttccat atattggaag ccttaagact
      241 tctcaatttt ttatcttctt tcttgtcctg tcgaaccata tactttgttg acatcaaaca
      301 tcgatttcct tttttgctac ccttctcttg agcaccaagg caaacccgag cgaggctgct
      361 gatagtaatg ctgacgtaaa gtatactaca tcatacgatt ctagagatga ataggacccg
      421 ggaactccct tgaccgtctc gtgactctct aggtacaccc ctgtcaaagc tggaccgatc
      481 gccatcccta tgaataacaa gagagcgcct actcctaccg atattccaat gaatgatttg
      541 ggtgatgatg acacgatgat gttccacgtt gcagtaattg tcattgagag accggatgca
      601 ataatggcta agtcggtaga tactgcgaat tccgtagagt gaaacatcag caatccgatc
      661 gatccgatga ggctgatcat ggcaccgatg atgatcggtc ttagtttccc tattcgattt
      721 atgataagcg gggtgacggc cgcaaaaact aggaacataa tcatgaaagg cagttgtacg
      781 tttgcggcat ctactgcatc ccctccaaat cccaaaggca ctgggcttct taccaactgc
      841 actatagctg gatatgccat gaacatcgta attccgctgg ccatcaatat gatgtacgaa
      901 ggcaggagag tcttgtcctt tagcaatcta aggtcgacca atggtgatgc aatttttctc
      961 tctatgatga caaaaacaga caatgaaatt acagaaacta taaatgcagc tccaatctgt
     1021 ggcaaagttt cagagtttat tccggtttct agaagtgtca gcgcaatgag aaaagcgatt
     1081 attgttgttg aaagtgctag tgcccccttc aagtctatgg aaggtgatgc ctgctttttg
     1141 ccggtgcttt cttgagtatt gacaggagcc agtaattgtc ctttgttttc ttttacgaat
     1201 tttgcgatca tgattgtcac tgtggctgca aatggaacta tggagaagaa agtgaaatgc
     1261 catccaaagc tttggatgat ggtagctcca gccaagaggc caacaaccga gccagctgag
     1321 tacatagatc caaacactcc tacggcgatc gcaagtttgg ctggtggaaa cgtgtctctg
     1381 agcaaactga aggctgccgg gactgccgcc aagcccacgc cctggattat cctgctcgct
     1441 aacaggaatg atatgttgtc tgcgaaccct ccagcaatta ttccagctgt ataaatcgtc
     1501 agtagtacga gtaatatctt ctttttaccg taaaggtcag acattttgcc ggcaatagga
     1561 gtcattacgg cggcaacaat taggtacgcg ctgaatatcc acgcagcagt cccataagat
     1621 atgctaaagt cctccattat ctctggaatg gctggcaaga gcatagtttc tgaaaaaaag
     1681 actaccaaga gcgtgctgct aagtattcca agagtaaacc aagcatgtcg gttgacatta
     1741 acggaagcta acgaagtcat ataaccatga taggtgtatg tgcaccattt ataagtgtat
     1801 ctacactcat ctatggttga gctcgagaca tatcaaacct agtaaacctt ttattgttaa
     1861 tatgtgtata tcaacctaga ggattgcagg aacgaaatat taatgcaata ctggaacaag
     1921 ggatgtcaac ggattcatgt acatgcggtg aattgcggaa ggcggctagg gcagtaacat
     1981 tactttatga taatgcattc aagtccagtg gattgctatc cacgcaactt gatttactct
     2041 atgtgatcta caagtcagac tctataagaa tttcacatct tgctaaagaa ttaggtatgg
     2101 ataggactac ccttactaga aatctgtccg ttttagaaag acagggcttt atcaagatct
     2161 cctctggtaa agataatcga actaggattg taacaattac ctacaaggga cgctccactg
     2221 tggccaaaac tattccgctt tggaatgagg ttcaa
//
LOCUS       DASQTF010000222         9138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_904048,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000222 DASQTF010000000
VERSION     DASQTF010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904048"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            497..886
                     /locus_tag="VE308_05285"
     CDS             497..886
                     /locus_tag="VE308_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZB00415.1"
                     /translation="MLANSPISPTIPVVDLDRAKRFYETTLGLKPVPANNDNTSGIAT
                     FECGKGTRIDLYQRGPSKADHTVATFEVSDTEEEVNALRGKGVVFEEYDMPEIKTQNG
                     IATHGPHKAAWFKDSEGNILCIHQLLR"
     gene            complement(1182..1439)
                     /locus_tag="VE308_05290"
     CDS             complement(1182..1439)
                     /locus_tag="VE308_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00416.1"
                     /translation="MNLRHVAIQLCTCTWIEIMTGAGITFYLLYLQSYHLAALTVVVF
                     APLIGFHFIIDRLSSNKLEPSGRLVRIKRCSQSKDQVISSA"
     gene            complement(1504..1674)
                     /locus_tag="VE308_05295"
     CDS             complement(1504..1674)
                     /locus_tag="VE308_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00417.1"
                     /translation="MALVISIPAIAVTLVMHYVIKTNLIVTMVAGLITLFMAMGFGYK
                     LSKKLTTKVQDG"
     gene            complement(1788..2252)
                     /locus_tag="VE308_05300"
     CDS             complement(1788..2252)
                     /locus_tag="VE308_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00418.1"
                     /translation="MERSSLREETKLDDKLDRLLNQFEREVEPYDRFSAFSMFSTPFG
                     IVLSIFLPLAYLHFVAGVNPFASMVSTGIIYWMVGGILATVGLTKLPIFYADHKKHEI
                     SRTKYKPIAGVCMCDLSQLRSHMKKMEKAKATGERMMHAKLVKYYKQQIGWE"
     gene            2565..2825
                     /locus_tag="VE308_05305"
     CDS             2565..2825
                     /locus_tag="VE308_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00419.1"
                     /translation="MTSQQSKLIFSNRRLENVSPDALIKSFWVSSALALIMTLPPLGI
                     FLAFHYNDGNILAGAVIGFGLHFVLLALSERISNALLRLFDD"
     gene            2883..3938
                     /locus_tag="VE308_05310"
     CDS             2883..3938
                     /locus_tag="VE308_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HZB00420.1"
                     /translation="MSSKVSMPSKIFGTVLSHGLRGRMLTKGELQTLAESRDIEELVT
                     RTKNTVYLDALAKLTKPYTAEKVEGALREYLVNMHAKMVTIAGGSTILDAYFVKYITR
                     NLKLVLKGKALGKTYEELLPKINLRAEELVGRRDLVIKALVAKDLDEAVASLAGSEFG
                     EDVAKAVAAYKEKRDVRLFDTYLDHAFYHALDRALVTDAQSQDVKKLVSTDIDSYNVL
                     AVLRGKFWGLSQQDITDLIVTTTPKVTRESIQKMINTEKLSEAIGELNNTVYRDIVPQ
                     NASNDIDAIMQLEAGFESVAHKRVVSSFRAVFTVAIMLSALKLMMIEVKNLAAIAAGV
                     EQNVPAETIMSRLVRLE"
     gene            complement(3987..6140)
                     /locus_tag="VE308_05315"
     CDS             complement(3987..6140)
                     /locus_tag="VE308_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HZB00421.1"
                     /translation="MGLAKLMKATVVLPRVDTPEVVSRLAELEWFHPIQTPSDHSNPY
                     LDDLLLKAQRLFQEIDEVVKDLGIPLETGVMATMFKGAPKGKTDYFIENIQNFIADLE
                     DKSAAILDNPKKMIAERNKVTKELEEYRNLKDALEMAAKVNLDLTLFGKFKYFFSGLF
                     VIDSTDEVEIRRTLGDLPIYTSKLSEHKLSMVVFGAADDSDRITKTLRSFGVHPLQIP
                     ANMPQNPSVAYAKAEAKVKELEAKQTEIEKNIEKLKESLLTKVLSLHEAAKVAKDVLE
                     TTRKPAGTRNFALIQGYIPDKMEKKFRSMAKDYVSVIEDVHLHSDKHGGEGEEEEEVI
                     LPTLLTNKSYTRTFEVITETQGIPRYGEVDPTPIIAFVWPAFYGLMFADFGHGILLFG
                     LGMLFRYRGNGSLRTWGTLIAASGAAAALAGLGTGEMFGFHFEELAILAPIFAPLGFV
                     GLLSVSELTFEQVVKILEISIAIGIVHLLMAYFLRLRADFKQDNKMRVFYHDIPTILQ
                     YFAVVALILAAIGSGYDIIGMFGITGVTHTEPVPWLTVVFGDWVTVDLVAKVAPLVII
                     ATVVIMIIGGIKEEKHLKAQGRDEGGGLVGIIVEVIMVRIIEMLSNTISYSRLGIMLL
                     VHSALLVTVNDAFEHGGGYAVLIGGNIGIMLIEGLIVYIQTIRLHLYEWFPKWYVGEG
                     TEFKKIVPKMLYSNFVWKDSEVQVQAKEMPAQAEK"
     gene            complement(6221..6550)
                     /locus_tag="VE308_05320"
     CDS             complement(6221..6550)
                     /locus_tag="VE308_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HZB00422.1"
                     /translation="MKKLNGRITISLLVVLPSIALAGLGGQAFAQESPAAGGDTGSSK
                     LIGAGMAFGLAALGAGIGLGFVGAAGLAAISDNPKMQSRVFIIVGMVESIAIYGIVMM
                     FIILGQT"
     gene            complement(6588..6875)
                     /locus_tag="VE308_05325"
     CDS             complement(6588..6875)
                     /locus_tag="VE308_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00423.1"
                     /translation="MKRDKKIIEEWKQVDRMKQLNQRLYDELDVALMFTLEYAERNNI
                     VLPNRDRLFRMVENIHNTTNAINSITVKSTTTRYKRIPNNGRKRPTNTTEP"
     gene            complement(7711..7836)
                     /locus_tag="VE308_05330"
     CDS             complement(7711..7836)
                     /locus_tag="VE308_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00424.1"
                     /translation="MTEEISYFEKAKAELKAEHDSMVEKIKGDIAASKRKALSKI"
     gene            complement(7833..8498)
                     /locus_tag="VE308_05335"
     CDS             complement(7833..8498)
                     /locus_tag="VE308_05335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HZB00425.1"
                     /translation="MSFGGRVTATKIELIKIRRSMQVAKMVHKILDDKREVLLKKIDE
                     MIEEANKAREDIWTPLDEIYTSVYDAYMSLGTMTLESISDSTPSIMEVDVNIRRIVDV
                     KIPTLKVETEKGRQQLSYGFVETNASVDKAAKLIKSMLPGICKAAEYENAIFSLAKEL
                     ERTQKLLNALEYVIIPQYQDAIYFIKATLEEREREEFVRLKKVKVVLDKKKMAEEQAS
                     ART"
     gene            complement(8512..>9138)
                     /locus_tag="VE308_05340"
     CDS             complement(8512..>9138)
                     /locus_tag="VE308_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HZB00426.1"
                     /translation="DMTNYAEALREISAAREEVPGRKGYPGYLYTDLANNYERAGRIK
                     GKNGSVTQVPILSMPADDITHPIPDLTGYITEGQIVLGRDLFRKGIYPPVNVLMSLSR
                     LMKDGVGEGKTRSDHMEVGNQLYDAYSRAQEVRALSEIVGRAGLTGVDLKYLDAGDKF
                     EQHFLKQAPDENRTLEETLTRAWDVLSGLPEGELTKIKEKHVRQYYKG"
BASE COUNT         2384 a         2136 c         1910 g         2708 t
ORIGIN      
        1 atctcttctt attgaaatac ataatattga ggatccgagc cattatgata acattgttga
       61 ctttctcaaa tatcataatt atgaaataac atttgagcag cgttatgaag gcagcggcga
      121 aagccacgta atatttcgta agaaaaatag taataataat aattcaaaaa atccttgacg
      181 atgtttctat acctataatg ctttggtacc atggttaact tattaacacc aacctttttc
      241 ttcagttggt tcattgccaa atatcctcgt ctattcttca aaatctccat gcattaagga
      301 gatgtttcag attgggacac aatatatagc gttatacctc aattaatgcg cgggtcacgg
      361 taatttgaca gacactgcaa aaaccgtacc tactcggttg tttgtttttt aatgtagatg
      421 tatcttctta tataatctta atctttaaaa attaacaaga ttctacaaat acttaatctc
      481 agattctatt tctaatatgt tagcaaattc ccctattagc ccaaccattc cagtggtgga
      541 tttggatcga gcaaaaaggt tctacgaaac aacactgggc ctcaaacctg tacctgccaa
      601 taatgataac acatctggca tagctacgtt tgaatgtggc aaaggcactc gtatagatct
      661 ctaccaacgg ggcccctcaa aagcagacca tacagtagct acatttgaag tgtctgatac
      721 agaagaggag gttaatgctt tgagaggaaa gggagttgtt tttgaagagt atgatatgcc
      781 tgaaatcaag actcagaacg gtatagcgac gcatggcccg cacaaagcgg catggttcaa
      841 ggatagcgaa ggaaacatcc tttgcattca ccaactacta agataaactt gcttgcattc
      901 aagctaaaaa agaaaaacct gtacctctga aaacttcttc atttacagcg gatgttctca
      961 ccatatttgt attgtacaaa taagaaacta ttatctgtgt ttgatctccc ttggcatctt
     1021 ttctttgtaa aagtatcatt atgtagtata tatgatacac ccatcttcat tgtatatcct
     1081 ggttgagcta atatgcaact taaggctatc aattagagaa aaaagtctga tctattaaaa
     1141 agtggattat gggaaatttc atgatttctt ttgctctttt tctaggctga actgatgacc
     1201 tggtcttttg actggctaca tctttttatc ctgacgaggc gaccgcttgg ctccaatttg
     1261 ttgctagaca acctgtcaat tataaaatgg aatccgataa ggggcgcaaa caccacgaca
     1321 gttagcgccg ccaggtgata gctctgcagg tacaacagat aaaacgttat gcctgcgcca
     1381 gtcattattt caatccaggt acaggtgcac agctgtatcg cgacgtgtct taaattcata
     1441 tgacttagag ttggtcactt caactatttg attttttcta gatcttggcc gtcatcacat
     1501 ctactaaccg tcctgcacct tagtggtgag ctttttggat agtttgtatc caaagcccat
     1561 agccataaag agggtaatca agcctgcaac catggtcacg ataaggttag tcttaatgac
     1621 gtaatgcatc acaagcgtca ccgctatggc aggaatggaa atgacaagcg ccaaaactgt
     1681 gctggcggca aaaacacggg ttgcagtgga tcttgtttgg cttgtcgctc atcatgattt
     1741 caactaggat tctaatttat tagcgtgatc ctgccgccaa tagaaattta ttcccagcca
     1801 atctgttgtt tgtagtattt caccagcttg gcatgcatca tcctttcgcc tgtcgccttg
     1861 gccttttcca tcttcttcat gtgagagcga agctggctga ggtcgcacat acacacacct
     1921 gcaattggct tgtactttgt tctcgatatc tcgtgctttt tgtgatcggc gtagaatatc
     1981 ggtagcttgg tcaggccaac tgtagccaat ataccgccaa ccatccagta tatgataccg
     2041 gttgagacca ttgaggcaaa cggattgact cctgctacaa aatgcaaata tgccagagga
     2101 aggaatattg agagcacaat tccaaacggc gtggaaaaca tcgagaaggc agagaaccta
     2161 tcgtaaggct ctacttcgcg ctcgaattgg tttagcaatc tatccaactt gtcatccagt
     2221 ttggtctctt ctcgaagaga tgagcgttcc atttgttatt acattgtctg aatggtaatt
     2281 attaagttta actgaacttt cttatttttt gataattcta caaaattctt atacagattt
     2341 aattttttta tacatttaga aagagtagct tggtataatt aacaaccatg attgttttaa
     2401 tttgacataa acgttgaaaa aattagctga agcgtccaaa ggacctaaaa attcatttta
     2461 ggacctcaat gtgacataat attgtacatt tatgttaata gagcttttaa catcttattg
     2521 tgtcaatatt aatatttcaa tctttctcaa taaaagttga gaaaatgaca tcacaacaat
     2581 ccaaattgat cttttcaaac cggcgacttg aaaacgtctc tcctgacgcg cttatcaagt
     2641 cattctgggt cagttcagcg cttgcactga taatgacgct tcctccgctt gggatattcc
     2701 tagcgttcca ttataacgac ggcaacattc ttgctggtgc cgttatagga tttggcttac
     2761 actttgtcct tcttgcactg tctgaaagga tatcaaatgc actattgcgt ctgtttgacg
     2821 actaaccttt tctcaccctc ataggctttt tatagagtat tggacgaaca aaggcatgca
     2881 ttatgagtag caaagttagc atgccaagca agattttcgg cactgtcctc tcacatggtc
     2941 tgaggggcag gatgttaaca aaaggtgagc tccagacgct tgcagaatcc cgcgacattg
     3001 aagagcttgt cactaggacg aaaaataccg tttatcttga tgcgcttgcc aagctcacga
     3061 agccctacac tgcggagaag gtggaagggg ccctgcgtga atatctggtc aacatgcacg
     3121 ccaagatggt cactatcgca ggcggctcta cgattctgga cgcttatttt gtcaagtata
     3181 ttaccaggaa tctaaagctc gtgctaaagg gcaaggcatt gggcaagact tacgaagagc
     3241 ttctccccaa gatcaacttg agggctgagg aactagtggg caggcgtgat ctcgtgataa
     3301 aggcgcttgt ggccaaggat cttgatgaag cagtagcctc gcttgcaggg agtgaatttg
     3361 gtgaagatgt agccaaggca gtggcagcct acaaggaaaa gagggatgtc agactttttg
     3421 acacttatct tgatcacgca ttctaccacg cccttgatag agcactggta accgacgcgc
     3481 agtcgcaaga cgtaaaaaaa ctggtctcaa ctgacattga ctcatacaac gtcttggccg
     3541 tcctccgggg caagttctgg ggattgagcc agcaagacat caccgacctg atagtgacta
     3601 ccacgcccaa ggttacgcgg gaaagcattc agaaaatgat taacacagaa aaactgtcgg
     3661 aggctatcgg agagctcaac aatacagttt accgcgatat tgttccgcaa aatgcatcaa
     3721 acgacattga cgcaataatg cagcttgaag cgggctttga gtcagtggcc cataagcgtg
     3781 tcgtcagctc ctttcgtgca gtgttcacag tagcgataat gctctcagcc ctcaagttga
     3841 tgatgattga agtaaagaac ctggcggcaa tcgcagcagg cgtggaacaa aacgttcctg
     3901 cagaaacaat aatgtcgcgg ctggtcaggc tagaataaaa aacccagcgt gtagtctata
     3961 tcttgtcaaa aattagaagt tatgcactat ttctcggcct gagccggcat ctccttggcc
     4021 tgaacctgca cctcgctgtc tttccagaca aagttactat agagcatttt aggcacgatc
     4081 ttcttgaact ccgttccctc gcctacgtac cacttgggga accactcgta caggtggagc
     4141 ctgatagtct ggatgtatac aatgagccct tcaataagca tgatgccaat gttgccaccg
     4201 ataagtaccg catagccgcc cccatgctca aatgcgtcgt tgacggtcac cagcagcgca
     4261 gagtgcacta gcagcatgat tccgagtcga gagtagctga tcgtatttga cagcatttct
     4321 atgatcctca ccatgatgac ttcgacgata attcctacaa gccctccgcc ctcgtctctg
     4381 ccctgggcct tcagatgctt ctcttctttt attccgccaa ttatcatgat gacaacagta
     4441 gcaatgataa ctagcggtgc gaccttggcc acaagatcta cagtaaccca gtcgccaaag
     4501 acaaccgtca gccacggaac tggctctgta tgggtaacgc ctgtgatgcc aaacatgcca
     4561 ataatgtcat agcctgaccc gatggctgca agtatcagag ctaccaccgc gaagtactgt
     4621 aggattgtcg ggatatcgtg gtagaacacc ctcatcttgt tgtcctgctt aaagtcggcg
     4681 cgaagcctca ggaagtatgc catcaacaga tgcactatgc ctatcgctat agatatctcc
     4741 agtatcttga ccacttgctc aaacgtcagt tcagacacgc tcaggagtcc aacaaagccg
     4801 agcggcgcaa agattggcgc aagaattgcg agctcttcaa agtggaaccc aaacatctca
     4861 ccagttccaa gacctgccaa ggcggcagct gcgccacttg ctgcaatgag tgttccccat
     4921 gttctaagtg atccattacc tctatatcgg aaaagcatgc ctagtccgaa caaaagtatg
     4981 ccgtgcccaa aatcggcaaa catcaatccg taaaaagccg gccaaacaaa agcgataata
     5041 ggcgtaggat cgacttcgcc gtacctcggt attccctgag tttcagttat cacttcaaag
     5101 gtgcgagtat acgacttgtt cgtaagaagc gtgggcagga ttacttcttc ttcttcttct
     5161 ccttctcctc catgcttgtc agagtgcaga tgcacgtcct cgattacgct gacataatcc
     5221 ttagccatgc tcctaaactt cttttccatc ttgtctggaa tgtaaccctg aattagcgca
     5281 aagttgcggg tgccagctgg cttcctagta gtttcaagca cgtccttggc aaccttggct
     5341 gcttcatgca aagaaagaac cttggtcaaa agcgattcct tgagtttttc gatatttttt
     5401 tcgatctctg tctgcttggc ttcaagctcc ttgaccttgg cttctgcttt agcataggca
     5461 acacttggat tctgtggcat gttggccgga atctggagtg ggtggacccc aaagctcctg
     5521 agtgtctttg ttatgcggtc agaatcgtcg gctgcgccga aaaccaccat tgataactta
     5581 tgctcggaga gtttgctggt ataaatcgga aggtcgccta gcgttcttcg gatctcgact
     5641 tcatctgtgg aatcaattac aaacaggccg gaaaagaaat atttgaattt gccaaaaagc
     5701 gtcaggtcaa ggttaacttt ggctgccatc tcgagcgcgt ctttaaggtt cctgtattct
     5761 tcaagctcct tggtaacctt gttccgctct gcaatcatct tcttaggatt gtccaagatg
     5821 gctgcgctct tgtcttccaa gtctgcgata aagttctgta tgttttcaat gaagtaatcg
     5881 gtctttccct ttggggcacc cttgaacatc gtggccataa cgccagtttc aagagggata
     5941 ccaaggtcct tgacgacttc gtctatttct tgaaaaagcc tctgtgcctt gagcaaaagg
     6001 tcgtcaaggt aggggttaga atgatcagaa ggagtctgga tgggatgaaa ccattcaagc
     6061 tctgccaacc tgctcaccac ttccggcgta tctacccggg gaagtactac ggtggctttc
     6121 attagtttgg ctagtcccat tcttgcctac cgttaatttc aatctcttta aaatctttcc
     6181 gtttgcaaat gaaaaaggag gggatagaaa aaaactttcc ttaggtctgg cccaagatga
     6241 taaacatcat gactatacca tagatggcga tcgattcgac catacctacg atgatgaaca
     6301 ctcttgattg catcttggga ttatcgctaa ttgcggcgag acctgccgca cctacgaacc
     6361 caagaccaat gcctgctccg agggctgcca ggccgaacgc catgcctgct cctatcagct
     6421 tcgatgaccc tgtgtcgcct ccagctgctg ggctttcctg cgcaaacgcc tgacccccta
     6481 agcccgctaa agcaattgag ggaagaacca ccaaaagtga aattgtgatg cgtccgttca
     6541 gcttcttcat gtaagagttc gagataaaat gccatattta actataacta cggttctgta
     6601 gtgtttgtcg ggcgttttct tccattgttg ggaatccgtt tgtaacgagt tgttgttgat
     6661 tttaccgtga tactatttat tgcattagta gtattgtgaa tgttctctac cattctaaac
     6721 aacctgtctc tatttggcaa cacaatgttg ttccgctctg catattctaa agtaaacatc
     6781 aatgctacgt ctaattcgtc atagagcctt tggttaagct gtttcattct gtcaacctgt
     6841 ttccattctt caattatttt tttgtcacgc ttcaaactca ttcaaggctc ttgccttcta
     6901 tgatctatct cctttgccac tattgctcac ggtgctggtg cctcttcttc actctgtgtc
     6961 tttagctctt ctttctaaat atctatcaat caaattgaga agggatgcac tccgagcgta
     7021 gattaatact gtttgtggta taccttggat tgcccttctg gaagtgtatc aatttgtttt
     7081 gatagtgcat caatattctt ccataaggca ttagacctct cattacatct atctttgaat
     7141 tcatccatcg gatgctggtc agacatattt ctaaaactca tctcctcgat tctcttacgt
     7201 ttcattttca tagaatgtct tgcttgttcg actgcgttaa cgtgaactta tgggggtagg
     7261 cttctcggtg acaggttcgt ttgtacctgt cttgattttg aatatcgtaa gattgacgct
     7321 tgtaagcaca tacatatgca gctaagctat agattgctgt caggacacaa ttacaaaatg
     7381 agtcaaagcc taaagtcttt gcagaagatg ctattccatg cactagatgg tcgctatttg
     7441 gaagcattct cttgattatt gttttgatga tattcttagg gatggctcta tacaataata
     7501 aaatagtcaa tcaatcaatc aatcaatcaa cccactcact cataggcaac tgattatcct
     7561 tataacgttg aaatctctat tccctaatca ttttacaata cctttcccat ttaccgctaa
     7621 ttcaaccaga ccaacggagt cgcaacaact gatttaaaca acggaagaac caacagaacc
     7681 ataactactt gaaaaaggtg aaaataagga ttagatttta gatagcgcct tgcgcttgct
     7741 tgccgcaatg tcgcctttga tcttttccac cattgagtcg tgctcggcct tgagctcggc
     7801 cttggccttt tcaaaatagc ttatctcttc tgtcatgtcc ttgcgcttgc ctgctcttct
     7861 gccatcttct tcttgtccag tactacttta accttcttga gcctgacgaa ctcctcgcgc
     7921 tcgcgctcct ctagagttgc cttgatgaag tatattgcat cttgatattg cggtatgatg
     7981 acgtattcaa gtgcgttgag cagcttttgt gtacgctcta gctcttttgc aaggctgaat
     8041 attgcatttt cgtattctgc tgccttgcat atgccgggga gcatgctctt gatgagcttg
     8101 gccgccttgt cgacagacgc gtttgtttcg acaaagccgt atgacagctg ctgcctgccc
     8161 ttctctgtct cgaccttcag cgttggtatt ttaacatcta cgatgcgcct gatgttgacg
     8221 tcgacttcca ttatactggg ggtcgagtcc gatattgatt caagcgtcat tgtgccaagg
     8281 gacatatagg cgtcataaac agaggtgtat atttcatcca agggtgtcca tatgtcttcc
     8341 cttgccttgt ttgcttcttc aatcatttcg tctatctttt tcaacaatac ttcgcgcttg
     8401 tcgtctagaa tcttgtgcac catcttggct acttgcatcg agcgccttat cttgataagc
     8461 tctatctttg ttgctgtaac cctacctcca aatgacatat ttctctactt ctcacccttt
     8521 gtagtactgc ctgacgtgct tttctttgat cttggtcaac tccccttcgg gcagacctga
     8581 gagtacgtcc catgctctag tgagcgtttc ttcaagtgtc ctattctcgt caggtgcctg
     8641 ctttagaaag tgttgctcaa acttatcgcc agcatcgagg tacttcaagt ctacgcctgt
     8701 aagacctgcc ctgccaacaa tctctgaaag cgccctgacc tcctgcgctc ttgaataagc
     8761 gtcatagagc tgattgccaa cttccatgtg atctgaccta gtctttcctt cgcctactcc
     8821 atccttcatt agccttgaaa gcgacatgag cacgtttact ggcgggtaaa tgccctttct
     8881 gaacagatca cgcccaagca caatttggcc ctcagtgata tatcctgtga ggtctgggat
     8941 tggatgcgta atatcgtcag ctggcataga taggataggt acctgcgtta cactgccatt
     9001 cttgcccttg atcctccctg cgcgctcgta gttgtttgca aggtctgtat agaggtagcc
     9061 ggggtaaccc ttcctgccgg ggacttcttc ccttgcagcg ctgatttcgc gaagcgcttc
     9121 tgcatagttg gtcatgtc
//
LOCUS       DASQTF010000223         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_905734,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000223 DASQTF010000000
VERSION     DASQTF010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905734"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1800)
                     /locus_tag="VE308_05345"
     CDS             complement(7..1800)
                     /locus_tag="VE308_05345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HZB00427.1"
                     /translation="MKVGRWDITDLVKDPSSKEFSQFLRSIEEQLVQFEERRQLLRPD
                     ILPEEFEGLIHMLETISEKVSIASGHAYLRYYADTSSNEASALVIKMDKLASEISNRM
                     LFFDLWFKKQIDHENANRLINAVPEAYQEHLRHKRLVAKYSLSEPEEKIISTLEVTGT
                     RALIKIYDKMTSGFEFTMKLRHGRKTIEKKFDNKEKLVSLVRSPDGNRREAAYKSLLH
                     VYKRNSGVLGEIYQNIIMQWRDEAILMRGYRSPISVRNIANNLDDVTVEALLAACRNN
                     VATFHDYFIEKAKMLGMKKLHRYHLYAPISKKASDSKKFTYGKAVEIVLDSFGNFDPQ
                     FRRLAERVFAEQHIDSEIRKAKRGGAFCYTVTPKLTPYVLLNFDGLSRDVSTLAHEFG
                     HAIHSMLASDMPLMVFHAPLPLAETASVFAEMLLNEKLSEKMSKKERRILLAEQIDNL
                     YATILRQAYFTLFEINAHKAIGENNATIDSVAKIYMDNLREQFGTAVTISPDFEWEWV
                     YIPHFYHTPFYTYAYSFGNLLVLSLYRQYKLEGKSSFVPKYFKILSAGGSRKPEELLM
                     EAGIDISREEFWQQGFDYVQEMIQQLKALAP"
BASE COUNT          507 a          443 c          436 g          627 t
ORIGIN      
        1 atgtcatcat ggagcaagtg ctttcagctg ctgtatcatt tcttgtacgt agtcaaatcc
       61 ctgctgccaa aattcttctc ttgaaatgtc aatgcctgct tccatcagga gctcctctgg
      121 cttcctggat ccgcctgcag acaatatctt gaaatacttg gggacaaaag acgatttgcc
      181 ttcgagcttg tactgcctgt aaagggaaag gacaagcaag ttgccaaacg agtaagcgta
      241 ggtgtaaaat ggcgtgtggt aaaaatgagg gatgtatacc cactcccatt caaaatcagg
      301 agatattgtt accgcggtgc caaattgctc cctgaggttg tccatgtata tttttgccac
      361 tgaatcaatg gtagcattat tttcgcctat cgctttgtgc gcgttaattt caaaaagtgt
      421 aaagtacgct tgacggagta ttgtcgcata aaggttgtct atttgctctg ctagcaaaat
      481 tctgcgctcc tttttcgaca tcttttctga tagcttttcg ttgagcagca tctcggcaaa
      541 tactgacgca gtttctgcaa gcgggagcgg cgcatggaag accattaatg gcatgtccga
      601 ggcaagcatg ctgtgtattg catggccaaa ctcgtgggca agagtcgata catcccttga
      661 aagtccatca aagtttagga gcacgtaggg tgtcagcttg ggtgtcacgg tatagcaaaa
      721 cgcaccaccc cgctttgcct tccttatttc agaatcaata tgctgctcgg caaatactcg
      781 ctcggccagc ctgcggaatt gagggtcaaa gttcccaaag ctatccagca cgatctcgac
      841 tgctttgcca taggtgaact ttttgctgtc agatgctttc tttgatatag gagcgtagag
      901 atgatatcta tgcagttttt tcattcctag catcttggct ttttctataa aataatcatg
      961 aaatgtggct acgttgttcc tacacgctgc cagtaatgcc tcaactgtta catcgtcaag
     1021 attgttggct atgttacgca cagagattgg agatctataa ccgcgcatca aaattgcctc
     1081 gtcacgccat tgcatgataa tattctggta aatctcgccc agcacaccgc tgttcctttt
     1141 atagacatgc aataatgatt tgtaggcagc ttctcttcta ttgccatccg ggcttctgac
     1201 gagcgatact agcttttcct tgttgtcaaa ttttttctct attgtttttc tcccgtgccg
     1261 aagcttcatg gtaaattcaa aaccgcttgt catcttatca tatatcttga taagtgccct
     1321 ggtgccagta acttcaagag tgctgattat tttttcctct ggctcgctga gcgaatactt
     1381 ggcgaccagc cgcttatggc ggagatgttc ctgatatgcc tctggaacag catttattaa
     1441 cctatttgcg ttttcgtgat cgatctgctt tttgaaccac aaatcaaaaa ataacatcct
     1501 gttactgatt tctgacgcta gtttatccat cttgatgact aatgcagatg cctcgtttga
     1561 agatgtatcg gcgtaatatc tcaggtatgc atggccgctt gcaattgaca ctttttcgga
     1621 gatggtttca agcatgtgaa taaggccttc aaactcttcc ggcaaaatat cggggcgcag
     1681 gagctgtcgc ctctcttcaa attgcacaag ctgctcttca attgatctca aaaattggga
     1741 aaattcttta ctagaaggat ctttgacgag atcagttatg tcccaccgtc ctaccttcat
     1801 attggcatag ctactagtca tatgggttac aggcacttac gatcttgccg tcacataagt
     1861 atcttgttga gcctgaggta aaaaattgcg gccaaaaaga gctatatggc ttattgctgt
     1921 gagtaaagct tactttgtat tcttggagca gatttggacg agaagccgaa tatatagtag
     1981 ctctttattt aaagtcttta ggatggaacg ttg
//
LOCUS       DASQTF010000224         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1197896,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000224 DASQTF010000000
VERSION     DASQTF010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197896"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VE308_05350"
     CDS             <1..301
                     /locus_tag="VE308_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00428.1"
                     /translation="AVFDHLKTLLTDKWFRVRINTCRALADAELITAIPDLTWVMEND
                     LDYRVRRVAQECINIIRRATRKPKEVAQMREELESLKSKNLELLQKVDRLERELR"
     gene            complement(565..1425)
                     /locus_tag="VE308_05355"
     CDS             complement(565..1425)
                     /locus_tag="VE308_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZB00429.1"
                     /translation="MITIRPARRSDKDEILSFCISTFNWGDYIDRVWDYWYTEKNGQL
                     FVVESGREKIAMSHVAICPEGIGIWLEGVRVRPDHRRSYIATMLLEKMLEYGRRKGAR
                     QASAIVGATNFASQSMMEKNGFKIVSKWMYYSALGTPNHQESNARIANPDDVGDIWQY
                     LQQSKMYRLSGKRYVKSWHWYEFDCRALLNFVKEERVIVTGRPIDGIAIINKCGYWER
                     ANVLQIVYLDIAPASSLRELFSFIMNLYLNGKFEGLQLVCYDSKRLSSFINKLVIKEH
                     EQFILYNKQI"
     gene            complement(2061..>2480)
                     /locus_tag="VE308_05360"
     CDS             complement(2061..>2480)
                     /locus_tag="VE308_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HZB00430.1"
                     /translation="QIATGYNGTPPGIKNCYEGGCLRCLARTKGEIKSGEGLDRCLCT
                     HAEANAIMQCALFGNAGSTKGAILYSTFAPCLECSKMAISVGIKRIVVLAYYPEDGTQ
                     LLRDANVDLVKLNSDLLKPWLSVITADPESSAKAVSK"
BASE COUNT          728 a          551 c          509 g          692 t
ORIGIN      
        1 tgccgtcttt gaccacctga aaaccctgct gactgacaag tggttccgag tccgcataaa
       61 tacgtgtaga gcacttgcgg atgccgaact gataacggca attcctgacc tgacatgggt
      121 gatggagaac gacttggact atagggtaag gagggtagct caagaatgta taaacatcat
      181 tagaagagca acaaggaagc caaaagaagt cgcacagatg agagaggagc ttgaaagcct
      241 caagtcaaag aatttagaac tactgcaaaa ggtggatagg ttggagcggg agcttcgcta
      301 actactgata acaaattctg caactacatc atgttgaagc gcgagggctt atcactcaca
      361 agaaacctgt aagataagca tgcaataacc tgcggtccta ttacgaactt tttcaatatt
      421 atttgtccac tttacataga gaagcaaagg caggtaatca aaaacaaagg attgataata
      481 cagacatcga gtatggcata tacacaggcc atcaacttcc aagtatctcc agtccaaaca
      541 taagtgtaag aaatgagcta caacttaaat ttgcttgttg taaaggatga actgctcgtg
      601 ttcttttatc actaatttat tgataaagga tgataatcgt ttgctgtcgt aacatactag
      661 ctgcagaccc tcaaatttac catttaagta aagattcatt ataaaggaaa aaagttcacg
      721 gagtgagctt gcaggcgcaa tgtcaagata gactatttgc agaacgttgg ccctctccca
      781 gtaaccgcac ttgttaatta tcgcaatgcc atcaatgggc ctgccagtca caattacacg
      841 ttcttctttc acaaaattta gaagtgctct acagtcaaac tcataccagt gccatgactt
      901 gacatatctc tttccagaca ggcgatacat cttggactgc tgaaggtact gccagatatc
      961 acctacatca tcaggatttg caattcttgc attcgactct tggtgatttg gtgtacctaa
     1021 tgcactgtag tacatccact ttgatactat cttaaaacca ttcttttcca tcatactctg
     1081 cgacgcaaaa ttggttgcac ccactattgc agatgcttgt cgagcacctt tccgcctgcc
     1141 atactctagc attttttcga ggagcattgt tgcaatgtaa gaccggcgat gatcaggacg
     1201 tactcttaca ccttcaagcc agatgcctat gccttcggga cagatagcaa catgtgacat
     1261 tgcaatcttc tcgcggccag actcgactac aaatagctgg ccattctttt ctgtgtacca
     1321 ataatcccac actctatcga tgtagtcgcc ccagttgaag gtacttatgc aaaaactgag
     1381 gatctcatcc ttgtctgatc ttctggcagg cctgatcgtt atcatcaagt tttcttataa
     1441 cacctagttc catcatgcgg ataagagtta ttatgagcta ctggatgttt aataaaatat
     1501 agagacctct acaagcaagt caatctcttg aatattatga caacatagaa atgtatatgt
     1561 caaactgtga cttatcgccg acatattttg tatgcaggct tgggatattg ttgtaaatgt
     1621 agtccagtat agcctacgcc aaaggacatt gctctgctgc tctgattcta agtctaagta
     1681 caatagaatg ggacatcact tttattgctt gtaagtatca gtttcaatgt ctacattcaa
     1741 ctgtagttca aacacagtca gtatacagtg gagaaattac atccatctct agttatggct
     1801 gcagcttaat ttgatatata gatcataaca tagaacattc caaatgccaa ggcgtttagc
     1861 tgaaggtttc tatcctaaaa ccctgcttaa aatggacctg ctgcattgca ccagtcacta
     1921 tagaataggc ttgtatgggg gcttgcgaca aagacctttg attcgcagaa tgcaggtgtt
     1981 cagcttgtca atgtcatcaa gtaagatatt tcaaggaaaa aagctgcgca cattaaatta
     2041 ggcaataggg gctagtgctc ttacttgcta actgcctttg ctgatgactc agggtctgca
     2101 gtaattactg aaagccaagg tttcaacagg tcagagttga gcttgaccag atctacattt
     2161 gcatctctca gcagctgcgt gccgtcttca gggtagtatg caagcaccac tatcctcttt
     2221 atgccgacac ttatagccat cttgctgcat tctaagcaag gagcaaaagt agagtagagt
     2281 atggcaccct ttgtgctccc agcgttgcca aagagggcgc actgcattat tgcattcgcc
     2341 tcagcgtgag tacacaagca gcgatcaagg ccttcgccgg acttgatctc accctttgtt
     2401 ctggcaagac agcgaagaca tccgccctcg taacagttct ttatccctgg cggcgttcca
     2461 ttatagccgg tagctatctg
//
LOCUS       DASQTF010000225         4621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1016617,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000225 DASQTF010000000
VERSION     DASQTF010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016617"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..652
                     /locus_tag="VE308_05365"
     CDS             224..652
                     /locus_tag="VE308_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00431.1"
                     /translation="MIATKKMAVLMAATILAAGISIAPFLGAEAESINTTRSNIKSSS
                     IAQQNADQVCTNQAQGQGGQQAVPNPEQTTIAERGDPVPDIDVKVGKNPGGSAAGAGI
                     SSEGDPTVDAEQSNECSIAMEQTVEQNGDTEDFSTNEWTE"
     gene            complement(815..1009)
                     /locus_tag="VE308_05370"
     CDS             complement(815..1009)
                     /locus_tag="VE308_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00432.1"
                     /translation="MLHDDIDEPENKKPQAVAPTTAITVLDNGPQVEALTNQGKELKE
                     TVNILICQIQGLRSEVRQKK"
     gene            1175..2983
                     /locus_tag="VE308_05375"
     CDS             1175..2983
                     /locus_tag="VE308_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZB00433.1"
                     /translation="MLGGLQTYATATVDTPAIDDPGTYMDGQRIATYEEMGIQSLLQK
                     YKNNTLDPAELINDIYDRIELNDDTDQNPILISRVSRDAALERVAELKELRNNLSPGI
                     DFTNQYPLWGIPFVVKDIVDVEGMATTVGSANWTNFPNCDYPPVSGLPGAPTPETLEF
                     KDPSLLRFDVAPEPETDCHPEPIANAPTAGPFANSTALIVQLALDKGAILIGKANLDQ
                     FATGLVGTRSSYGPVRNVRNDDYITGGSSSGSAAAVALGWASFSYGTDTAGSGRVPAG
                     FNDLVGYKPTPGLLSTHLTFPAVRSVDANTIFSLNVSDARYVAEQIKQYDDTDPYSRP
                     EADTLECTGKEAPARFKFGVPRELAEPNLSLYMDNRLNDLWANDTGAREQYFAAVERL
                     QRIGGEPVLFDYKPWEDVARLLYGGASVAQRFLTYGNYVLDNRPTGEPITMNPDTDQL
                     IDPTTYLIFKNAFYNKATRAYADEWQINLIKQQVISKDWQRFDFMLLPSTPTIYTLDE
                     VRAGYEQDLGPNATDAQLTEKAAANTQLNSNLGTYTNFANLLQTTAVAIPAGFRSIND
                     GDILPFGVQLFGDTLNDCEVLALAERYEAELAREQD"
     gene            complement(3230..3748)
                     /locus_tag="VE308_05380"
     CDS             complement(3230..3748)
                     /locus_tag="VE308_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00434.1"
                     /translation="MSPQPIWDEQATSTSVTPINETLRVATFIGNGTMTVPDTGETIN
                     MTNNGTIIASFVPQANNTTISYGRGNIFSVDDGDTSANTFSDIVQYDPTTFQGRGLVT
                     AVFDDNATGSLAPFNGMLVVVGTHEEDPSTQAATIRLREWETGLTLPPVGTTTTPMQQ
                     TPLSPMNTTTPC"
     gene            complement(3776..4063)
                     /locus_tag="VE308_05385"
     CDS             complement(3776..4063)
                     /locus_tag="VE308_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00435.1"
                     /translation="MNATSSTTEEGNQAIGEVAIHIQEACLAAHNKDIPGVLMHLNLA
                     LDALNAVRGGDAANATAALEERGLILEGGPEGGEVGAGFTVPSGPRYGVPS"
     gene            complement(4060..4212)
                     /locus_tag="VE308_05390"
     CDS             complement(4060..4212)
                     /locus_tag="VE308_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00436.1"
                     /translation="MLEAVAADSPSPKMTPLTTNPAASAAAQEEQQEVSLQAAEQQQA
                     AQLLRT"
     gene            complement(4430..>4621)
                     /locus_tag="VE308_05395"
     CDS             complement(4430..>4621)
                     /locus_tag="VE308_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00437.1"
                     /translation="VGRGIANPQDYLLAASFQAYLKAQNKRNVKQIICHAKIIAVSGN
                     RPIAVSSLCHIYRRELCNV"
BASE COUNT         1313 a         1019 c         1020 g         1269 t
ORIGIN      
        1 acagttcttt agcggtaagc attaggatga caattaacag tatcgtatgg gttgcatttt
       61 aatggttaaa cttcaacaaa gcctgaaata ttttcaatgc acttattaag taaagcaatt
      121 taactctcac tagaatttag acataaatta tctatactgc aagtgagaat aaaaaaccgt
      181 agagcttcta ataaatcata actttttatg gcacatcacc tatatgatag caacaaaaaa
      241 gatggcagtc ttgatggctg caacgatctt agcagcaggc ataagtattg ctccttttct
      301 tggagcagaa gcggagtcaa taaacacaac tcgatctaac ataaaaagct cgtctatagc
      361 acagcaaaat gctgaccaag tgtgtacaaa tcaagcacaa ggtcagggtg gacaacaagc
      421 tgttcccaat ccagagcaaa ccacaatagc tgaacggggc gacccagtac ctgatattga
      481 tgtcaaagtc gggaaaaatc ctggtggatc tgcagcgggt gcaggaatat ctagtgaggg
      541 cgacccaact gttgatgcag agcagtcaaa tgaatgctct atagcaatgg agcaaacagt
      601 agaacaaaat ggtgacacag aggactttag caccaacgaa tggacagaat aatcaaccaa
      661 tcaatcaatc aaaaaataag aaagatagat gaatcgaagg gagaaggaga caaaatattt
      721 gcttctccca cttagctttt ttatgcaata ttgcatatac ttttttgtag cagcaactgc
      781 cacggttctt caacagagaa aggccgtaaa ttattcactt cttctgccta acttccgacc
      841 ttaatccctg aatctgacag atcaaaatat tgacagtttc cttgagttct ttgccctggt
      901 ttgtcagagc ttcaacctgt ggaccattgt ccaagactgt tattgctgta gttggtgcta
      961 ctgcctgggg ctttttattc tctggctcat caatatcatc gtgcaacaca tcagttatga
     1021 taataaccca atcgggatac tcttacgctt tagtgattaa atacttctcg agtttatacg
     1081 acagcccttg tcgactacaa gcaccaattc gaatcgaaga atgtatgtag ttcttgctgc
     1141 tgttttttcc gcatttatac ttatgctatc ttctatgtta ggtggacttc aaacatatgc
     1201 cactgcaact gtagacacac ctgccattga cgacccaggc acttacatgg atggccagcg
     1261 gattgcaaca tatgaagaga tgggaatcca gtccctgttg caaaaataca agaataatac
     1321 gctagatcca gcagagctga tcaatgatat ctatgacaga attgagctca acgatgacac
     1381 cgatcagaat ccaattctga tttcccgggt ttctagagat gccgcgcttg agagagtagc
     1441 cgagctcaaa gagttgcgga ataacctttc tcctggaata gattttacca atcaatatcc
     1501 gctttggggt attccctttg tcgtgaaaga tattgtagac gttgagggta tggcaaccac
     1561 ggtaggcagt gcgaattgga caaacttccc taattgcgat tatccaccag tttcaggctt
     1621 gccaggtgct ccgactccag agacactgga atttaaagat ccttcactgc tgagattcga
     1681 cgtggcacct gaaccagaaa cagactgtca tcctgagccc atagcgaatg ctccaaccgc
     1741 gggaccattc gcgaattcca ccgcattgat cgttcaactt gctctagata aaggagcgat
     1801 cttgataggg aaggcaaact tggatcagtt cgccacagga ttagttggaa cccgttcctc
     1861 gtatggtcct gtacgtaatg ttcgcaacga tgactatatt acagggggat cgagctctgg
     1921 gtctgcagcg gcggttgctc ttggatgggc aagtttctcc tacggaacag atacagccgg
     1981 ttcaggtcgt gtacctgcag ggtttaatga cttggttgga tacaagccca cgcctggact
     2041 gcttagtact cacctcactt ttccagctgt aagatcggtt gatgcaaaca cgatttttag
     2101 cctgaatgtt agtgatgcaa gatatgtcgc agaacaaatc aagcaatacg atgacacaga
     2161 tccatactcc agacccgaag ctgatactct ggaatgcacg gggaaagagg ctccagcaag
     2221 gttcaaattt ggtgttccaa gagagcttgc cgagcctaat ctatcacttt acatggataa
     2281 cagattaaat gacctgtggg ctaacgatac tggagctaga gagcaatact ttgccgccgt
     2341 tgagcgcttg caaaggatag gaggagaacc agttctgttc gattataaac cctgggaaga
     2401 tgttgcaaga cttctatacg gcggtgcatc ggttgcgcag cgctttctaa cgtatggcaa
     2461 ttatgtgctt gacaataggc ccactggtga gcccattaca atgaatcctg atacagatca
     2521 gctgattgat cccaccacgt atctgatttt caagaacgct ttctacaata aggcgactag
     2581 ggcgtacgct gatgaatggc aaatcaactt gatcaagcag caggtgatta gcaaggattg
     2641 gcagcgattt gatttcatgc ttctaccctc tactccgacc atttacacgc tggacgaagt
     2701 aagagctggt tatgagcaag atcttggacc aaacgcaacc gatgcacaac ttactgagaa
     2761 agctgctgca aacactcaac tgaactcgaa tctaggaacc tatacgaatt ttgcgaacct
     2821 gttacaaacg acagcagtcg ctatccctgc gggattccgt agcataaacg atggtgatat
     2881 cttgccgttt ggagtacagc tgtttggaga cacgttgaac gactgtgaag tgttagcact
     2941 tgcggaacgt tatgaagcag aactagcaag ggagcaggac tgagtagcaa gtcgtgtata
     3001 ccgaggggat taaaaaggac taaggttttt ctaaacccgt atcttttttt ataattttga
     3061 ggtaggcttg tagacagcgc tttttgccac tttatctgta aagtactatg tagagaagta
     3121 gcagcagcag caaagatgat aacaacgaca acgacaaagc aaaagcttta gaaatcttaa
     3181 aggatttgta aaagaataaa aaagataaaa aggatgaatt aatggtcagt tagcagggtg
     3241 ttgtcgtatt catcggagaa agaggggttt gttgcatagg agtagtagta gtaccaacag
     3301 gtggtaatgt taatccagtt tcccactccc gtaatcttat agtagccgcc tgagtacttg
     3361 gatcttcctc atgtgttcct actactacta acatgccatt gaatggtgca agactgccag
     3421 ttgcgttatc gtcaaatacc gctgttacaa gtcccctgcc ctggaaagtc gtagggtcgt
     3481 actgcacaat gtcagagaat gtattagctg aagtatcacc atcgtctact gagaatatat
     3541 tcccccttcc ataagaaatg gtagtattat tagcttgagg cacaaaactg gcaattatag
     3601 taccgttatt agtcatattg atagtctcac cagtatcagg aacagtcatc gtgccatttc
     3661 caatgaatgt tgcgactcta agtgtctcgt ttattggtgt aacgcttgtg cttgttgcct
     3721 gttcatccca gattggttga ggtgacaatt ctaatctttc tatctggttt gaatactacg
     3781 atggaacgcc ataccttggg cctgatggca cagtaaaccc agctccgact tcaccgcctt
     3841 caggtccgcc ttctaatatc aggcctcttt cttcaagtgc agcagtagca ttagcagcat
     3901 cgcccccacg tactgcattt aatgcatcca aggctaaatt caagtgcatc agcacacctg
     3961 ggatgtcctt attgtgcgct gccagacatg cttcttgtat atgtattgct acctcgccta
     4021 tggcttgatt tccttcctct gtagtactac tagtggcatt catgtccgta gtagctgcgc
     4081 cgcctgttgt tgttccgctg cctgtaatga tacctcctgc tgttcctcct gcgccgccgc
     4141 cgatgccgca gggttagtag tcaacggcgt catttttggc gatggcgagt ccgccgccac
     4201 tgcctctagc actaacttct atagacttca gattccttaa acacttctag tagtattcat
     4261 atactctcat acaaataaaa attgtctaaa atcttaacat acccaaagtg tatttgaatg
     4321 agtgcatgag cctccaagtt cctagaggga ctcatgtaat cgcatcctcg ccagttggtc
     4381 atctttggat cagctggcgg gttttggtac ctgttagaag ctgtcgcttt tatacattgc
     4441 ataactctcg tctgtagatg tgacaaagac ttgacactgc aatgggtcgg ttcccagata
     4501 cagcaattat ctttgcgtgg caaattatct gtttgacatt tcttttgttc tgtgctttga
     4561 ggtaagcctg aaaactagca gcaagaaggt agtcttgcgg attagcaatc ccacgcccta
     4621 c
//
LOCUS       DASQTF010000226         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_1273355,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000226 DASQTF010000000
VERSION     DASQTF010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273355"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VE308_05400"
     CDS             complement(<1..1045)
                     /locus_tag="VE308_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00438.1"
                     /translation="MVENSQIQLVKKPTAVNLNRFVKPIDINRLVSQYGTPLYLIDED
                     TLHAKAKELHKAYSKFKGQVKVAYSIKANFTPAVVKAFMKDGIAFDLTSLGELYFIKQ
                     CKADPENVIYTSVTEELEEYIEVLQSGVRRIVVSSFNGMTNLAKAASKVGGGAVPLTM
                     IRVNPEVGVKAEVRASYRNSKFGVPFNGGTIDSAAKMVKHLLGSDLLKFDGFHFHLGS
                     QITDFSCYTHALDKMDVFITKMKKEDPTFQVNTFDIGGGTPIFYNDPVPTPDQMAENH
                     ISKLNQLAETHGTFTLMIESGRFLVAESSILVSKIVNTKEYNEHKIVIVDAGYHILLD
                     AALLKQEYPQEVVP"
     gene            complement(1192..>3303)
                     /locus_tag="VE308_05405"
     CDS             complement(1192..>3303)
                     /locus_tag="VE308_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HZB00439.1"
                     /translation="GRGVARIDYDSMDSLGASTGDVIEIRGKRRTVAKCLPLYPSDEG
                     KGIIRVDGLVRNNAGIAIGDTVIVKKIKAVPAEKVIVAPLEAIPPIDERYLADALESV
                     PLIKGDNVMVPYFGGRLTFQVIGVTPAVDAVLVTQKTVFHIAEKGETLRGVPQVTYED
                     IGGLKEEIQKVREMIELPLRHPEIFEKLGIEAPKGVLLYGPPGTGKTLLAKAVANESN
                     AHFISISGPEIMSKFYGESEARLREIFKEAKEKAPSIIFIDEIDSIAPKREEVTGEVE
                     RRVVSQLLSLMDGLEARGKVIVIAATNRPNALDPALRRPGRFDREIEIKVPDKRGRLE
                     ILQIHTRNMPLEADVDQDRIAGVTHGFVGADLEYLCKEAAMKCLRRVLPELNLEDEKL
                     SPEVLNKLIVTMSDFESAIKEVMPSAMREVYLESPDIPWSAIGGLEEVKRELQEAVEW
                     PLRYPDLYTKLGHTMPKGLLMHGPSGTGKTLLAKAVATESEANFISVRGPELLSKWVG
                     ESERGIREIFRRARQAAPCVVFFDEIDSIAPTRGMGGDSMVTERVVSQLLTELDGIQA
                     LSGVVVIAATNRADMIDPALLRPGRFDKIVFVPMPDKAARQRILEIHAKDKPMGPDVD
                     FVKISEMTEGFSGADTSAVANTAVSLVLHEYLAKYPTPEEAAKHASEAHVILRHFEEA
                     VRKIKTQREGKPAEKAVPYYR"
BASE COUNT          749 a          831 c          692 g         1031 t
ORIGIN      
        1 ctggtactac ttcttgtggg tactcttgct ttagtagtgc agcatcaagt aggatatggt
       61 aacctgcgtc cacaatcact atcttgtgct cattatattc cttggtattc actatctttg
      121 aaacaaggat tgacgattct gccactagga agcggccgct ttcgatcatc agagtaaatg
      181 taccgtgtgt ctctgctagt tgattcagct tgctgatgtg gttttcagcc atctggtctg
      241 gcgttggcac agggtcattg tagaatattg gcgttccgcc accaatgtca aatgtattga
      301 cctgaaaggt ggggtcctcc ttcttcatct tggttatgaa tacatccatt ttgtccagcg
      361 catgggtata gcatgaaaag tcagtgattt gagatcctag gtgaaaatga aagccatcaa
      421 acttcaagag gtcgcttccc agcaaatgct tgaccatctt agctgcgctg tctatggttc
      481 cgccattgaa tggcactcca aacttgctat ttctgtatga agcgcgaact tcggccttga
      541 cgcccacttc cgggttcaca cgtatcattg tcagtggtac tgctcctcct cctaccttgc
      601 ttgcagcctt ggctaagttt gtcatcccat taaaagaact gacgacaatc cttctaacgc
      661 cgctttgaag cacttcaatg tactcttcta actcttctgt gacacttgta tagattacat
      721 tctccgggtc tgccttgcac tgcttgataa agtagagctc gccaagcgac gtcaggtcaa
      781 atgctatgcc gtccttcatg aacgctttga ctactgcagg tgtaaagttg gccttgatcg
      841 agtacgctac tttgacttga cccttgaact ttgaatatgc tttgtgtagc tcttttgctt
      901 ttgcatgaag tgtatcttcg tcaatcagat aaagtggtgt accatactgt gagaccagtc
      961 tgttgatgtc aatgggctta acaaagcgat ttagattaac tgctgttggt ttttttacga
     1021 gttggatttg agaattttca accaatttct tttagttagg tgtatcataa aaccaataac
     1081 ttaaaaacat ttgccctcta gcaaaagagt tcgttctgta tgtatgtggg aaataaattt
     1141 tcagaaatgg acaacaaaaa aagagaaaga agaaaaaaga agtcagctgg cttaacggta
     1201 gtatgggact gccttttctg caggcttgcc ttccctctgt gtcttaatct tcctcactgc
     1261 ttcttcaaaa tggcgtaata tcacatgtgc ttctgatgcg tgtttggctg cttcttccgg
     1321 cgtcggatac tttgctagat attcgtgtag cacaagtgag actgcggtat ttgctactgc
     1381 acttgtgtct gctccactaa aaccttcagt catctcagag atcttaacaa agtccacatc
     1441 cggccccatc ggtttgtctt ttgcatgtat ctccagtatt ctttgtcttg cggccttgtc
     1501 cggcatcggg acgaacacta ttttgtcgaa cctaccggga cgcaaaagtg ccgggtcaat
     1561 catatctgct ctgttggttg cagcgattac taccactcct gaaagcgcct gtatgccgtc
     1621 aagctctgtc agcagctgcg atacaacccg ttctgttacc atactgtcgc ctcccatacc
     1681 tcttgtcgga gcaattgagt ctatttcgtc aaagaacaca acacatggag ctgcctgcct
     1741 tgctctgcga aagatctccc tgatgccgcg ctctgactcg ccgacccact tactcagtag
     1801 ctcgggtcct cttacgctga tgaagttagc ttcagactct gttgctactg cctttgcaag
     1861 taatgtcttg ccggttcctg atggtccatg catcaggaga ccttttggca tggtgtgtcc
     1921 aagctttgta tacagatcag gatatctcag cggccactca actgcctctt gaagctcacg
     1981 ctttacctct tctaagcctc caattgcaga ccatggaatg tctggcgact caagatatac
     2041 ctctctcatg gcagatggca taacttcctt gattgcactt tcaaagtcgc tcatggtgac
     2101 tatcagcttg ttgagcactt ccggcgatag cttttcgtct tcaagattaa gctctggtaa
     2161 tacccttctc aaacacttca ttgctgcctc tttgcagaga tattctaagt ctgcgccaac
     2221 aaagccatgc gtcactcctg cgatcctatc ttgatccacg tctgcctcaa gtggcatgtt
     2281 gcgtgtatgt atttgcagta tctctaacct gccgcgctta tctggaactt taatttcaat
     2341 ttctctatca aacctgccgg gtcttctcag agcagggtcg agtgcatttg gtctgtttgt
     2401 ggctgcgatg acaatgactt ttcccctcgc ttcaaggccg tccattagcg atagcagctg
     2461 cgacacgact cttctttcta cttcgcctgt aacctcttcg cgttttggag cgatagaatc
     2521 gatctcgtct ataaatataa ttgatggcgc cttttctttt gcttctttaa atatttctct
     2581 cagcctagct tctgattctc catagaattt gctcatgatt tcaggtcctg agatgctgat
     2641 aaagtgggcg ttgctttcgt ttgcaacagc cttggccaga agcgtcttgc ctgtgcctgg
     2701 agggccatac aacaatactc cttttggcgc ttctattcct agtttctcaa atatctccgg
     2761 gtgcctgagc ggaagctcga tcatttctcg aactttttgg atctcttctt ttaggccacc
     2821 gatatcttcg tatgttacct gcggtactcc tctgagagtc tcaccctttt ctgcaatatg
     2881 gaataccgtc ttctgtgtga ctaaaacggc atctacagcc ggcgtgacac caattacttg
     2941 aaatgtcagc ctgccaccaa agtatggaac catcacgtta tcacccttga ttagaggcac
     3001 gctttcaaga gcatctgcca gataacgctc gtctattggt ggtatagcct caagaggtgc
     3061 cacaatcact ttttcggctg ggacagcctt tatcttcttg acaattacag tatcgcctat
     3121 cgcgatgcca gcattgttcc taacaaggcc gtctaccctg ataatgccct tgccctcatc
     3181 agaaggataa agcggtagac atttagcaac cgtcctacgc ttgcctctga tttcaataac
     3241 atctccagtt gatgcgccca gtgaatccat tgagtcgtag tcgattcttg ctactccccg
     3301 acc
//
LOCUS       DASQTF010000227         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_272131,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000227 DASQTF010000000
VERSION     DASQTF010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272131"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VE308_05410"
     CDS             <1..288
                     /locus_tag="VE308_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZB00440.1"
                     /translation="ATVAAGIDGIRQKTDPGDPVNEDIYKMSDSARRALGIKMLPRTL
                     QDSIEALENDKAYLKPYFCNELIDTYVELKGEEIAFATGSKERQFMLYYDI"
     gene            688..1077
                     /locus_tag="VE308_05415"
     CDS             688..1077
                     /locus_tag="VE308_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZB00441.1"
                     /translation="MVSVVGASFYYVYATDAVEPWLGIEGSNVTPAIAQTLGLQESVG
                     FLIFSVEPSSPAELAGLKGGDRVVTIDGRPVLLGGDVITAVDNIQIDDAQDIEAELID
                     KTVGDVIEFSVIRGSSTQDVDVKLGAR"
     gene            complement(1764..2375)
                     /locus_tag="VE308_05420"
     CDS             complement(1764..2375)
                     /locus_tag="VE308_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00442.1"
                     /translation="MIYLRFSKVEILHLVIATALVTAVGLSLYRFQFRWDFLAIFISA
                     FVLHELGHKFLAQFYRAWAEFRVLFYGAVVTAISALPFFPFKFIAPGAVMISGNLSEA
                     RNGKVSLIGPLTNLAMGTGFLCVYLVLAAVDSAIPILLVGVRFNGFIGFFNMIPFMGL
                     DGQKIFGWNKIVWVLTMAGAIGLFIGGDLLSGGATTGFLRRFF"
BASE COUNT          808 a          577 c          595 g          734 t
ORIGIN      
        1 gcaacagttg ctgctggaat tgacggcatc aggcagaaga cggatccagg tgatccagtt
       61 aatgaagaca tctacaaaat gtctgattca gcacggagag ccctgggaat aaagatgctt
      121 cctcgcacat tgcaggattc gattgaggcg ctagaaaatg acaaggcgta tctaaaacca
      181 tatttttgca atgaactgat agacacatat gtggaactca agggcgagga gatagcattc
      241 gcgacaggtt ccaaagagcg ccaattcatg ctttactatg acatctagca tattatctct
      301 ttgtaggcct tacgagtata gagatcgcta ttgacctcag caccggtatt aggtcccaaa
      361 gttaaacctc tattatcttt ctaatagtta tcttgaggag ttgaatcaga agaactagaa
      421 aaaatttgct gaggtggcct cctgtgatgc aatctccatc gccaatatgc ttcttgtggg
      481 tagcacacaa acatagaaca ctccgagttg tatcatcttt tacattacag tggtacaagg
      541 agcaacatgc agatttgtgg gtccacaaag tacgcaaaac acttattctc agctttgcac
      601 tattattcga aaaggttatt gcctcaagat tcgagataaa atggtgaatt gcataatcct
      661 gtcaatactg tcatttcggt tgctgtgttg gtttcagtag ttggagcttc attttattat
      721 gtttatgcta ccgatgcagt ggagccatgg cttggaatag aagggagcaa tgtcacaccg
      781 gctatcgcgc aaacacttgg cctgcaggaa tctgtgggct ttctgatctt ttctgtcgaa
      841 ccatctagcc cagcagagct agctggattg aaaggaggcg acagggttgt aactattgat
      901 ggaaggccgg ttctgcttgg aggagacgtt ataactgctg ttgataatat tcaaattgac
      961 gatgcacaag acatcgaggc cgagctgatt gacaagacag taggcgacgt tattgagttt
     1021 agcgtaatca gaggaagttc cactcaagat gttgatgtta aactgggtgc gaggtgattc
     1081 atctgctgca aaatattata ggtcgattaa aggcactata attgattctg gattattcca
     1141 ttaaaaattc gtttttataa tgaaaaaact aattgggcag caataatggg agacgttata
     1201 atcaagcata ttgagcaagt cagattaaga cttcaccaag gactcgtact gtctagctct
     1261 atagagcctg cattgaaagt tttaatatac aaacacctca ataaattttg gtaaacattg
     1321 aactacgcta agcaagcatg atactgctca ggcacactgt attgaagatt catagactct
     1381 gtagtgatgt cgatgctgat atctggatac ttcagttatg ctacttgaga atgtctcaaa
     1441 tcgatgcatt gcgctaactc tgccccgtgc tgctatactt tatgtagagt tagtctgcaa
     1501 gaaattacaa tcttgggcaa gaacgtcttt aaaagacaga tcgaccctaa cattaaagcc
     1561 gtatatagaa aaaagccgtt tggtcatttt actggacgct tctgttcacg ttttatggtg
     1621 atctaatcga cttgttttgg gtgcaaggat tggcctctct atcagactct cttattcctc
     1681 ttttctttac catttaatac cgccttattg ctcgaccaat tcaggctgtt tggattttac
     1741 ccgctacaca aaattgagag ttgctaaaaa aatctcctca gaaagcccgt tgtggcgcct
     1801 cctgaaagca ggtctcctcc tatgaagagc cctatcgcac ctgccattgt cagcacccag
     1861 acgatcttgt tccatccaaa gatcttctgg ccgtcaagcc ccatgaaagg tatcatgtta
     1921 aagaaaccaa taaagccatt gaatctcact ccaactagca gaattgggat tgcagagtca
     1981 acagcagcaa gcacaagata gacgcacaag aagcctgtgc ccattgctaa gttagtgaga
     2041 ggtccaatca atgaaacctt gccgttgcga gcctcactga gattgccact aatcatgacg
     2101 gcccccggcg ctatgaactt aaacggaaag aatgggaggg cagaaatagc cgtgaccact
     2161 gcgccgtaga acaacacacg aaattctgcc caagccctgt agaactgtgc aagaaacttg
     2221 tgtcccagct cgtgtagaac gaatgcactg ataaagatgg cgaggaaatc ccatctgaac
     2281 tggaacctgt acaaggacaa cccaacagct gttaccaggg ccgtcgctat caccaagtga
     2341 agaatttcga ccttcgagaa gcgcaggtag atcatattat acagggaaga tctaatgcgc
     2401 atttgcggag aagcagctgt tccgtctatc cgtcccagaa attcacgatc ctttccaggt
     2461 tgctcgaggt aatcctgcag aaaaaagcag ttatggttgg ggggcaaccg atgaatagag
     2521 cagaaggtct ctttgcagta gcggcatctg aaaggcattg attcttttgt gccacacaaa
     2581 tcacaatgca tcgagtgcag tatcccatat gcattaaccc ctattaaacg aagaggatta
     2641 ttgttgtcta tacatcgcca tgcacaattt atattcttaa taaaaaatcg tttatcactt
     2701 caaataacgt tcca
//
LOCUS       DASQTF010000228         3558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_91907,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000228 DASQTF010000000
VERSION     DASQTF010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91907"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(707..1327)
                     /locus_tag="VE308_05425"
     CDS             complement(707..1327)
                     /locus_tag="VE308_05425"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HZB00443.1"
                     /translation="MSATNIAPEIKDKLVEHLNEMLSAENAAIDRLDSRIRECLLPEG
                     KEQLQHHQDETRAHQERLRQIITDQGGSPTDSKADLPTLRLPTGMLAKKTLTDVAKSL
                     TGGGGGDTNPMPEELELMHTKEDYGIEHVEIIAYRTLIQLCERLGISNAIPLLKQSMQ
                     EEESMAKWIETNLPMTLDKIWPRIEAALTGRSKEQTIPTGEKTATL"
     gene            1885..2424
                     /locus_tag="VE308_05430"
     CDS             1885..2424
                     /locus_tag="VE308_05430"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HZB00444.1"
                     /translation="MTTNGLFVGRFQPFHKGHLATVKFALSRVDQLVIVVGSAQKSHE
                     YRNPFTAGERIRMIKESLDADDEADVRRILIIPVPDIDVHSLWTHQVDMLVPKYDVVF
                     ANDLFTLMLFHEQGIKAIEAPLYRRNEMRATEIRKRMAAEENWEDLVPKPVSKVIKEI
                     NGIERVRAISQKDMPGHLI"
     gene            2462..2869
                     /locus_tag="VE308_05435"
     CDS             2462..2869
                     /locus_tag="VE308_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00445.1"
                     /translation="MSLMLFGNKYGSKEGGQQASSFINEPWYIDLQKLTNEELMRMYT
                     MISDGRYRFAKNAWYIPIEKDHRCPIMVAKGYRSPDTLDRMAEFQDTAKLLESDFLHF
                     IDAWDFGYITEQDIEKAILKILESRNIAITEHS"
     gene            2903..3508
                     /locus_tag="VE308_05440"
     CDS             2903..3508
                     /locus_tag="VE308_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZB00446.1"
                     /translation="MDHISPTFLIDLIRSTIALFVVIDPVGSVPVFVALTQKMERAEK
                     ASVTKTAIITAAGLLFLFAVAGTQILSIFSITISSFMVAGGILLFIVSIELLTHGEWR
                     FAGAGTQGESGIVPLAFPLLAGPGAITSVMISFQTAGLIVTALSILIVIGITYVVLRY
                     IDRIYRVIGRRGSIIVTRVFAVIIAAIAVQFIVDGARTLFS"
BASE COUNT         1034 a          692 c          752 g         1080 t
ORIGIN      
        1 aacgatatgg caagagcaaa aacaaagagt ccagcgaaaa caagatccaa gaaagctgca
       61 gctggaagaa aaggaggtaa aaaaggtggt cgcacatcag cggcaactaa aagaaggaag
      121 gtcggcaaaa gagaagcatc caccagtgct acaggatctg atgccgtgac cacaggaact
      181 acaacataga caaaaagata gtcaaaggaa gaaaaattag tggaagcatg aatccatttt
      241 aactatctaa attttttagt ggttgcaagt ctactgtgca gtatcatccc cctatagaat
      301 cattgtgact gtttcaggtt attttgatgt tgggctgcag tgcttgggtg gtgtcggctt
      361 gattatattt ctctctttgt tgactataaa tcgtcagaga aataatctga aacaatcaat
      421 tgaattagtg ttagtgattc aatgggatat taaggcgaag gtgtcagatg acctgctgca
      481 atgtgacctt cataagcact agcattatca aattttactc cacatatttt gcatttgaaa
      541 agaccgtctt ccgttatctc tgttgtgtct tgtacatctt tgttgttatc atctatgtta
      601 ctcaccatac ttgatcacat gttacatttg ctcttcctgc catcgggttc agatttcaat
      661 tcggtatcat gtagggggca tgggtctaat atatgcagag aggagattat agagttgcag
      721 ttttttcacc agtagggata gtttgctctt tacttctccc agttaatgca gcttcaatcc
      781 ttggccagat cttatcaagc gtcattggaa gatttgtttc aatccatttt gccatcgatt
      841 cttcttcttg catactttgt tttagcagtg gaattgcatt gctaatacct aacctttcgc
      901 acagctgaat aagcgttcgg taagcaataa tttcaacatg ctcaatacca tagtcttctt
      961 ttgtatgcat aagttccaat tcttcgggca taggatttgt atctccccct cctccccccg
     1021 taagggattt agccacatca gttaaggtct tttttgccag cattcctgtt ggcaacctta
     1081 atgttggcag gtctgctttg gaatcagttg ggctaccgcc ttgatcagtt atgatttgtc
     1141 ttagcctttc ttggtgtgct cgcgtctcat cttgatgatg ttgtaattgc tccttgcctt
     1201 ctggcaatag acattcccgt atccttgagt caagcctatc aatcgccgca ttttcagcag
     1261 acagcatttc attaagatgc tccacaagtt tgtctttaat ttccggcgca atattagttg
     1321 cagacatagc agctacagat ggcggccatt ggccttaata cttgtctata ttgcctacat
     1381 ttcataatat gataagaagc cctatgaatt tttttgtgta taatgaagtt atcagattca
     1441 ttatcttgtt aaacaattct cttatgcaaa taaaaatcca tttttaacat tattgaagta
     1501 aattttattc tataacagga ttttagtaat attacaatat aacaaactag tcaattgtaa
     1561 attcaatacg tccctttttt gagcagcaaa cgatcgtacc cttgtgatct cagaatttct
     1621 gttgtctgac aacaaatata ccgccaagaa tgtgatcggc tatgcaaagg taataatcct
     1681 gttcaatgca acctcaggtc agataatgct aaggaattct taccatcaat cctaaatcaa
     1741 acgctactaa cgtcgagtaa aggatgaaaa atacggtaaa gatcatttgt attaaaggca
     1801 ttatacttac tatagccttt tcaactggcc acgaagcttt cttaaaaata tgtttaaaat
     1861 agtgtcaact ctagcatatc gctgttgact actaatgggc tttttgttgg caggttccag
     1921 cccttccata aggggcacct tgcgactgtt aaatttgcac taagcagggt cgaccagttg
     1981 gtaatagtag tcggaagcgc gcaaaagagc cacgaataca gaaatccatt cactgcaggc
     2041 gagaggataa ggatgataaa agagtcgctt gatgctgatg atgaagcaga tgtcaggcgc
     2101 atactgataa ttccggttcc cgatattgat gttcactcac tttggaccca tcaggtggac
     2161 atgcttgtac caaaatacga tgtcgtgttt gcaaacgacc tttttacgct catgctattt
     2221 catgagcaag gcatcaaggc aattgaagcg cctctttaca ggcgcaatga gatgagggct
     2281 accgagataa ggaaaaggat ggctgcggaa gaaaattggg aagatcttgt tcccaaacct
     2341 gtctcaaaag tgataaaaga aataaatggt attgaaagag tcagggcaat ttcccagaaa
     2401 gatatgccag gccacctcat ctaagtagaa aataatatat ttctagctga ttgaccatac
     2461 aatgtcactg atgttgtttg gcaataagta tggtagcaaa gaaggaggac agcaagcttc
     2521 aagctttatt aacgaaccgt ggtatattga cctgcaaaag ctaacaaatg aggagctgat
     2581 gcgcatgtat actatgatat ctgacggccg ttatcggttt gccaagaatg cttggtatat
     2641 cccaattgag aaagaccacc gctgtcccat catggttgcc aaaggttaca ggtcgccgga
     2701 tacactagat aggatggcag agtttcagga tacagcaaag ttgttggaaa gcgactttct
     2761 ccattttatc gatgcatggg actttggcta cattactgag caggatatcg aaaaggcgat
     2821 cctaaagata ctggaatcaa ggaacattgc aataactgag catagttaat attaataata
     2881 catcgtcgcc aatacacaca atgtggatca tatttctccc acttttctta tagatcttat
     2941 cagatcgaca attgcactct ttgtagtaat tgatccagtt ggaagtgtgc cagtgtttgt
     3001 tgcacttact caaaagatgg aaagagcaga aaaggcatct gtcaccaaga cagcgatcat
     3061 aactgcagct ggtttgctct ttctatttgc agtggccggc acgcaaatac tatctatctt
     3121 tagcatcacc atctctagct ttatggtggc cggcggcatc ttgcttttca ttgtatcgat
     3181 tgagcttctt actcatggag agtggagatt tgccggtgca gggacgcagg gggaatcagg
     3241 tattgtcccg ttagcatttc cgctgcttgc aggccctggc gctataactt cagtgatgat
     3301 ctcatttcag acggcaggtc tgatagtgac tgctttatcg atcctgatag tgataggtat
     3361 tacctatgtc gtgttaaggt atatcgatag gatttataga gtgattggaa gacgaggctc
     3421 aattattgtt actagggttt ttgcagtgat catcgctgct attgctgtcc aattcatagt
     3481 ggatggagcc cggacactct ttagctaaga aagatttgtt aatatattta gcaattgact
     3541 ttgaaagcat tgacaaag
//
LOCUS       DASQTF010000229         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_913343,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000229 DASQTF010000000
VERSION     DASQTF010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913343"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VE308_05445"
     CDS             complement(<1..429)
                     /locus_tag="VE308_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZB00447.1"
                     /translation="MVTEKGEKRQGRRPLIGYLSVLFAAVLFGSVFSLAKLPLATIDP
                     LALTAIVYTISGLALIPFARASFTFERRDDYFYVVIVTIFGGVLAPVLLMYGLQQTAA
                     STAAILTNGEIVFTIAISSIFFGEKPHGRAGLFAVILLVIG"
     gene            619..2136
                     /locus_tag="VE308_05450"
     CDS             619..2136
                     /locus_tag="VE308_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00448.1"
                     /translation="MFSRSVPEINDPATNTPNDEAMEQRFEQQFCGSNTSPNSNAYIT
                     EVTLPTGCEMPVGIAIDGAKVWYVSTKRGLLGSYDSATGTFAEYEIPSWPTRLLPFTA
                     VPSWSMSWSVKLDGSGGNVWFTDQNNTIWRFNQSTEDFDMFKVPAKYPSMMDFDSNGN
                     LYFIGINSQSLFFGNASSIQNATSEGFREISLPLEGFSGISQDLITSGGLVIDREQND
                     VWVSLLAFQQKKGQLFQYDIETDTIVRIVDLPPELGAPVGMELDNYGKVWVADHGTST
                     FFNYDPTMDNMTTFVTSTASPKIYGGNTPPNAYTLPYWIDKGGDGSLWFNEHTGNKIA
                     RFDPEELALVEYWVPSQNRAWTLCPPEATACGIANVMQFAVDPMNQLWFSEWTENKIA
                     KLNASKQVPLAVLTPAEVAVAKGESTDIKVIVTASSDFTGEMMVAGTFMPNGALGNST
                     GIFSEESVTVPSGGSKEILYTFTAADNLEQGQYMIMLGAGNDDVSYMKAVKVNII"
     gene            complement(2255..>3391)
                     /locus_tag="VE308_05455"
     CDS             complement(2255..>3391)
                     /locus_tag="VE308_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZB00449.1"
                     /translation="VDFNVSINNGAIGEDYRIRMSLPTIEYLAKRGAKVILASHLGRP
                     KGHDKNYSVAPLAKHLTEIIGRSRPPIKFASDCVGVEIEENVSKMNKGDILLLENLRF
                     HKEEESNDPEFSKKLASLADIYVNDAFSTSHRKHSSTYGAAWMFDIRLAGFNLIKEVK
                     YLSMIRENPIRPFSIVLGGVKIKDKIGALEHLLPKADKVIVGGAAAYTFLKANGLNTG
                     NSIIDQEHIQWVEKALSTYGDKILLPTDHICASSPTDTSVTMVSGDIPLGMSGFDIGN
                     ETVERYSAEVGSNGGGALFWNGPMGMFEIKVFSNGTINIAKSMALAFWRGSKTLIGGG
                     DTLEAMKRAGVAENEVSHVSTGGGATLRYLAGDDMPGLVILNEH"
BASE COUNT          955 a          786 c          724 g          926 t
ORIGIN      
        1 acctatcact aataaaatta ctgcaaataa tccagccctt ccatggggtt tttccccaaa
       61 aaatatagat gaaattgcta tcgtaaatac gatttcaccg tttgtcaata tggctgccgt
      121 agaagcagct gtttgctgaa ggccatacat caaaagcacc ggcgccaata ctcctccaaa
      181 aattgttacg attactacat agaaatagtc gtcccttctt tcaaacgtga atgaggctct
      241 tgcaaaagga atgagagcta gccccgagat agtatatact atcgctgtta gagcaagagg
      301 gtctattgta gcaagaggaa gcttcgctag gctgaatacg gagccaaaaa gaaccgcggc
      361 aaacaatact gacaggtagc ctatgagagg tcgccgcccc tggcgctttt ctcctttttc
      421 tgtaaccaag caaatttgct aataatcctg atcggtattt ctgcctatac ctccgacaag
      481 agatataagg tggaaaagct ggtgtttttc tagccgttgc cagattcatc ccagaaaaag
      541 atcaggagcc gatcgtcaaa aaaaattatt atttatgcta taatcgggat tgcggccgtt
      601 gctgcagtct atatttccat gttttcacgc agcgtccccg aaatcaatga tcctgcaaca
      661 aatacgccaa acgatgaagc gatggagcag cggttcgaac aacaattctg cggcagtaat
      721 acctcgccta attccaatgc ttatataact gaggttactc tgcccactgg atgtgaaatg
      781 ccagtcggca tcgccattga tggcgcaaag gtgtggtatg tatcaacgaa gcgtggcctt
      841 cttggcagtt atgatagtgc cacaggtaca ttcgctgaat atgaaatccc ttcatggcca
      901 acccgtttgc ttccctttac agccgtacct tcgtggtcga tgtcatggtc ggtaaagttg
      961 gatggcagcg gcggaaatgt ctggttcacc gaccaaaaca atacaatctg gcgctttaac
     1021 cagtcgacgg aggattttga tatgttcaaa gttccagcca aatatccttc tatgatggac
     1081 tttgactcaa atggtaactt gtactttata ggaataaact ctcagtctct tttttttggc
     1141 aatgcatcca gcatccaaaa tgcaacctcg gaaggattta gagagatttc gctaccgctt
     1201 gagggattct ccggcatcag tcaggatcta attacttcag gcggattagt aattgatagg
     1261 gagcaaaatg acgtgtgggt ttcactcctt gctttccaac aaaagaaagg gcagttgttt
     1321 cagtatgata ttgaaactga cacaattgtg cgcatagtag atctaccgcc ggaacttggc
     1381 gcacctgtgg gtatggaact tgacaattat ggaaaagtct gggtggcaga tcacggcacc
     1441 agcacattct tcaattacga tcctactatg gacaatatga caactttcgt aacatcaaca
     1501 gcatcaccca agatctatgg cggcaataca cctccaaatg cctatacact tccatattgg
     1561 atagacaaag ggggtgacgg ctctttgtgg ttcaacgagc atacaggcaa caagatagcc
     1621 cgctttgatc ctgaagagct tgcactagtc gaatattggg tcccttctca aaatagagcc
     1681 tggacactat gtcccccgga agcaacagca tgcggcattg caaatgttat gcaatttgca
     1741 gtagatccaa tgaaccagct atggttcagt gaatggacag aaaacaagat cgccaaattg
     1801 aatgcatcaa agcaggtgcc acttgcagta ctaacaccgg ccgaagttgc cgttgcaaag
     1861 ggtgaaagca cagacatcaa agtgatagta actgcatcga gcgatttcac tggtgaaatg
     1921 atggttgccg gaaccttcat gccaaatggg gctcttggta actcaactgg tattttcagc
     1981 gaagagtctg tcactgtccc atcgggaggt tcaaaagaaa ttttatatac gtttaccgca
     2041 gcagataatc tagagcaagg tcaatatatg ataatgcttg gagcaggcaa cgatgacgtt
     2101 tcgtacatga aggcagtcaa agtcaatata atctaaaaat gcaatcttgc gataagagaa
     2161 agtaaaaaaa gtgtataaat tgtactcatt gcagtctcgt tgacgataat gattataatt
     2221 gaattacgag gtattgccgt gcatgaagta aagtctagtg ctcgtttaga atgactagac
     2281 caggcatgtc atctcctgct agataacgca gtgttgcgcc gccgccagtt gatacatgac
     2341 ttacttcatt ctcagcgacg ccagctctct tcattgcttc aagtgtatcg ccgccgccta
     2401 tcaaggtctt gcttccacgc cagaatgcaa gagccatgct cttagcgatg tttatagtac
     2461 cgttagaaaa tacctttatc tcaaacatac ccatggggcc attccaaaac agagcaccgc
     2521 ctccatttga acccacttct gctgaatagc gctcaactgt ttcattgcct atgtcaaagc
     2581 cagacatccc aaggggtatg tcgccgctaa ccattgtcac cgaagtgtct gttggggaac
     2641 tagcgcaaat gtgatcggta ggcagcaata tcttgtcacc ataagtggaa agtgcctttt
     2701 ctacccattg tatgtgctcc tgatctataa ttgagttccc agtgttgaga ccgtttgcct
     2761 tgagaaaagt atatgctgct gcaccaccaa ctatgacctt atctgccttt ggcaacaaat
     2821 gctcaagcgc tccaatcttg tcttttatct ttactcctcc tagcacaatg ctaaagggcc
     2881 taatcgggtt ttcccttatc attgaaaggt actttacttc ctttatcaga ttaaagcctg
     2941 cgagccggat atcaaacatc catgctgcac catacgtcga agaatgcttc ctatgagacg
     3001 tactgaatgc atcattgacg tagatgtcag caagagaagc aagttttttg ctaaactctg
     3061 ggtcgtttga ctcctcttcc ttgtggaaac gcaggttctc aagcagcagg atgtctccct
     3121 tattcatttt gcttacgttc tcctcaattt ctacccccac acagtcgctt gcaaacttta
     3181 ttggtggtct tgatcttcca atgatctctg tcaagtgctt ggctagcggt gcaacagaat
     3241 aattcttgtc atgtccttta ggccttccca ggtgcgaagc caatatgacc ttggctcccc
     3301 gttttgccaa atactcaatt gtcggaagag acattcggat tctataatcc tcacctatag
     3361 ccccgttgtt tatgctgaca ttaaaatcaa c
//
LOCUS       DASQTF010000230         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_551639,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000230 DASQTF010000000
VERSION     DASQTF010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551639"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VE308_05460"
     CDS             complement(<1..1148)
                     /locus_tag="VE308_05460"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HZB00450.1"
                     /translation="MFSTMFHTSLKPPIIETIPLLSKYKTKVWIEAYGCSASMADSEM
                     MSGLLKEAGYEIATKKSESALNLIVTCSVKDTTEHRMVSRIRTMTKSGKPLIVAGCLP
                     KADRAKVESVSSSASLLGPNSIDRATDAVRLALSGGRLVALRDSPLDKVNVPRVRLNP
                     LISIVEIASGCMSECTFCQTKIAKGWLRSYRIGDIVRQIKSDIKSGCKEVWLSSTDNG
                     CYGRDIDTNLVELLKACCSIEGSFMIRVGMMNPMYLPAILDRMVDLFCENDRLFKFLH
                     IPVQSGSDRVLKKMKRGHISKTFMDAVEAFRRRIPEMTISTDIIVGFPSETEDDFKET
                     VGLVERSQPDIVNISRYAARPGTKAYGWKDMRVNSQVSKERSECMHALT"
     gene            complement(1300..1686)
                     /locus_tag="VE308_05465"
     CDS             complement(1300..1686)
                     /locus_tag="VE308_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00451.1"
                     /translation="MSEDPPRLEQTASAIEDLLYMGAIRLVDKEDRALLSPQFSLVVS
                     NMMSSMKIDEGADSSEIMKLMYYSLLIYVGEYLKVPKSFMMALGNDLEKNRDSMESGA
                     LVTTYVTVLTEIWVHNRQQQQDGKNR"
     gene            complement(1711..2094)
                     /locus_tag="VE308_05470"
     CDS             complement(1711..2094)
                     /locus_tag="VE308_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00452.1"
                     /translation="MSDSIDAILQLPQRKIVIAENDVFLDKQIKNDIFILMVEESIGS
                     AGGRAASFGHRRVDRIYGFSCYGSGECTKFFDTIEQDKIDHFDIPYSAVAMDIRLSDG
                     KQYVLQGIVDPDSVASYRSVISNLK"
     gene            complement(2361..2588)
                     /locus_tag="VE308_05475"
     CDS             complement(2361..2588)
                     /locus_tag="VE308_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00453.1"
                     /translation="MDYYGKESTSSNKNKLLPIILLVASAAAIVAMFILPFFGVYFFF
                     FFLPLTFSLPWSIKRLRQYKARNQRNVVDLR"
BASE COUNT          725 a          666 c          587 g          838 t
ORIGIN      
        1 gtaagggcgt gcatgcactc tgatcgttcc ttggatactt gagagttaac cctcatgtcc
       61 ttccatccgt atgccttcgt tccgggcctt gcagcatagc gtgaaatatt aacaatgtcc
      121 ggttgagatc tctcgactaa gccaacagtt tctttgaaat catcctccgt ttccgatgga
      181 aatcctacaa ttatgtcagt agagatcgtc atctcaggga tcctgcgtct aaatgcctcc
      241 acggcatcca taaatgtctt ggagatgtga ccacgcttca tctttttcaa tactctgtcg
      301 cttccacttt gtacagggat gtggagaaac ttgaagagtc tgtcattttc acagaacaaa
      361 tccaccattc tatccagtat cgcaggcaaa tacataggat tcatcattcc tacccttatc
      421 atgaagctgc cttctatgga gcagcatgcc ttcagaagct ctacaaggtt ggtgtctatg
      481 tctcttccat agcatccatt gtcggttgag gaaagccaca cttctttgca accacttttg
      541 atatcagatt tgatctgtct cacaatgtca cctatgcgat agctccggag ccaccccttg
      601 gcaatttttg tctggcaaaa ggtgcactcg ctcatgcatc cgcttgctat ctctactatg
      661 cttatcaggg ggttaagcct gacccttgga acgttgacct tatcaagtgg agaatctcta
      721 agcgccacga gcctgcctcc tgaaagggct aaccttacgg catctgtcgc cctgtcgatg
      781 gaattgggcc caagaagact tgctgaggaa ctcactgatt ctacttttgc cctgtctgcc
      841 tttggcaggc aacctgcaac gatgagtggc ttgcccgact tggtcattgt cctgattctg
      901 ctcaccatcc tatgctcggt tgtatctttg acagagcatg tgacaatgag gttgagggca
      961 ctttcactct tcttggttgc aatttcatag ccggcctcct ttaagagccc actcatcatc
     1021 tcagaatcgg ccatacttgc agaacagcca tatgcttcaa tccacacctt agtcttatac
     1081 ttgctcaata gagggattgt ttctatgatt ggcggcttga gcgatgtatg aaacattgta
     1141 gaaaacatgt aaaccagctt accttaaaaa tcatatgcta tgtctatcgt ccgttttctg
     1201 atttacagac cagccaggaa tagaaatgag agtcaatgaa tgaataacaa gcaccttcac
     1261 agcagggatg tgtgagcttt caattgattg ctgcattagt cacctatttt ttccgtcctg
     1321 ctgctgctgg cgattgtgaa cccaaatctc agttaacaca gtaacataag tagtgacaag
     1381 cgcgccagac tccatgctgt cacggttttt ctccagatcg tttccaagcg ccatcataaa
     1441 agacttaggc accttcaggt actcgcctac atagatcagc agcgaatagt acatgagctt
     1501 cattatctcg ctgctatcag caccctcgtc gattttcatg ctcgacatca tgtttgaaac
     1561 tacaagggag aactggggag ataacagtgc cctgtcttcc ttgtcgacaa gcctgatcgc
     1621 acccatgtaa agcaagtctt ctattgcaga ggccgtctgc tcaaggcgcg ggggatcttc
     1681 tgacatgcta gttgttcttg gtacgcaagc ctattttaga ttgcttatca ctgaacgata
     1741 ggatgccaca gaatcgggat ccactatccc ctgcaacacg tactgcttgc cgtctgagag
     1801 cctgatgtcc attgcaactg cgctgtatgg tatgtcgaag tggtcgatct tgtcttgctc
     1861 gatagtgtca aagaattttg tgcactcgcc gctaccatag catgagaaac catatatcct
     1921 gtcgactcgc ctgtggccga agctggccgc tcttccaccc gcagaaccaa tagattcttc
     1981 caccattaga atgaaaatat cgttttttat ctgcttgtcc aagaaaacat cattctctgc
     2041 tatgacgatc ttgcgctgtg gaagctgtaa gatggcatca attgaatctg acatctgtgc
     2101 acctaatgca cagacaaagg agtaattatt atttattcaa gatctttttt ttgatatgct
     2161 atgctttttt gactactgca atgaatagtg attgccatgc atccagcctc cacaatatcc
     2221 ttatttgtcg aagacgggta cgtgcatggg gctttttcta tgaaaagcat ttcttgtcaa
     2281 gttgcagcaa ttgtatgtct ctctctgatt ctaggaaaga atcttttctt ctggttttgc
     2341 atagaactct tttgcattta ctagcggaga tcaactacat tccgctggtt tcttgccttg
     2401 tactgtctga gcctttttat tgaccacggc aaactgaagg ttagcggtag aaagaaaaag
     2461 aaaaagtata ctccaaagaa tggaaggata aacatggcaa caattgctgc agcagatgct
     2521 acaagcagga ttattggcaa gagcttgttc ttgttactgc ttgtactctc ctttccgtaa
     2581 taatccatcg tattattatt taggccaata tttataatgg taatgcgggg aaattcgtaa
     2641 tagatatata atatctacaa attgcatcat ttggcatggt caagactgct tcatcacccc
     2701 aagttctcaa gacaggcagt agggcaccgg attttacact gaaaggagta gatagtaaaa
     2761 tgcactcact acaggattac aagagcaagg cggcacttgt tgtgttcatt tgcaat
//
LOCUS       DASQTF010000231         3751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_913929,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000231 DASQTF010000000
VERSION     DASQTF010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913929"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VE308_05480"
     CDS             <1..697
                     /locus_tag="VE308_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00454.1"
                     /translation="STTNNNSNVVLGSLFLTGEDKLTSFNPINETYTEVSYVGNRTIM
                     PTDDTTTTTINATETGNLSLKLQPNGITFVEGQSLLVTEGRRGNNNGGAEEQENATAV
                     LVDLNGVRPDDPRSSTGVVFFSTNSTGQLAFLDNMIAVYQIKASPEGTAIRMWEWKGA
                     DLPFGNEGDDGITTTPKEPSLMNTTMTNATADTNATAAPEEEAEGNEEGEDPNEFEDC
                     VVPPGRDPGDVGC"
     gene            complement(1019..1273)
                     /locus_tag="VE308_05485"
     CDS             complement(1019..1273)
                     /locus_tag="VE308_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00455.1"
                     /translation="MGEKARKRYQENKNKGSKTIPDQQIREPVYDFTKPEAQVKVVGP
                     RDREGSGIGVNDYPSESIVKENTSTKNDEVKLRITSDKEC"
     gene            1330..1983
                     /locus_tag="VE308_05490"
     CDS             1330..1983
                     /locus_tag="VE308_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00456.1"
                     /translation="MTGALALVSDRYLKNDERFTHAKVTIGKSYKDVIDSLADKSMLP
                     YHPTIYPIPFMLSRRYKTGKELARALFYSLSYPDVLEFTKFSIYIVVSVFEKFPDHLV
                     GLIGHEIAHVIAAKGEVRLSEEDLRLILRSWLDFIEAKERTASRIYSYFRKPTKSMIE
                     MWNVVALQPETERAIAEGIQLVDKEQFDKLIFGERIADFNRFIELNLDRIRGSGSPS"
     gene            complement(2454..3575)
                     /locus_tag="VE308_05495"
     CDS             complement(2454..3575)
                     /locus_tag="VE308_05495"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZB00457.1"
                     /translation="MSEAKEETILVTGTSGRIGYAIAKRLAEDQQSFNVIGFDRKVPS
                     HPLPSAECLYVDLTDEESTRRGLQAIRDLHGDRIASVIHLAAYYDFSGAPSPLYDKVT
                     VGGTRRLLRLLQDFKVEQFIFSSTILVHAPSRQGVRINEDSPLDIPSKWAYPKSKIES
                     ENVIHAERGNIPVVVLRIAGVYDDLCHSIPLALQIQRIYERDPVAYFSPGDPSTGRHP
                     YVHNDDVADAILLAFALRKELSPEVVLLIGETKSLSYDDLQRAFGQLIHGVKWNTRSI
                     PPLVAKTGLRLLKFLALGRSASIAPWMIDVAQDNMELDITRARTLLGWNPQHSLLDTL
                     PKMVSGLKADPFTWYRENELKLPLWLRKQMPVAVTTGIS"
BASE COUNT         1044 a          866 c          839 g         1002 t
ORIGIN      
        1 gagcaccacc aacaacaatt caaatgtcgt tttggggagt ctgttcctaa ctggagaaga
       61 taaattaaca agcttcaacc caatcaatga aacctacact gaggtatcat atgtaggtaa
      121 cagaacaata atgcctacag atgatactac tactacaact ataaatgcaa cagagacagg
      181 aaacctctca ctaaaactcc aaccgaatgg tattactttt gttgaagggc aaagccttct
      241 tgtgactgaa gggcgaagag gaaacaataa tggtggcgca gaagaacaag aaaatgcgac
      301 tgccgtcctt gttgacctta atggagtaag acctgatgac ccaagaagtt caactggtgt
      361 tgtatttttt agcacaaact ctactggcca gctagcattc cttgataaca tgatagcagt
      421 atatcaaatt aaagcctctc cggagggaac tgctattcga atgtgggaat ggaaaggtgc
      481 agaccttcca tttggaaacg agggcgacga cggcatcact actactccta aagaaccctc
      541 tctaatgaac acaactatga caaatgctac tgctgacact aatgcaacag cagcacctga
      601 ggaagaagcg gaaggcaacg aagagggaga ggatccgaat gaatttgagg attgcgtagt
      661 tcctcccgga agggaccccg gtgatgtcgg ttgctgacac acgcacttac ctaaaaaatg
      721 gccggatatt atttttcatc ctgtgcctat gattcatcga gtccatgctc tgaagttttt
      781 ttctgccgga accctctggg cctgctttta tatcaataac atttgataga tgacctttct
      841 tatgtgcttc agatatgttt tattatcaag aatgcggatg tttgacacaa taatagtaat
      901 actcgttttt gcagtcaact gactaccaag tcaacaacct tcaggtacaa gaaagaacat
      961 ggctaataat atatagaagt aatattaagg tcaacgagaa gaagtgtcag agttagtttc
     1021 agcactcctt atcagatgta attctcagct ttacctcatc attttttgtt gaagtattct
     1081 ctttgacgat ggactcgctt ggataatcgt ttacgccgat accggaacct tccctatctc
     1141 gtggacctac aacttttacc tgagcttcag gttttgtaaa gtcatagacc ggttcgcgga
     1201 tttgctggtc tggtatagtt ttcgagccct tattcttatt ctcctgatat cttttccttg
     1261 ccttttcacc cataggccac tgaatatatc tgctccatat accttattta gattgaatac
     1321 aacaaggacg tgacaggcgc gttggcttta gtgtcggaca ggtatcttaa gaatgacgag
     1381 cgattcacac atgctaaagt gaccatcgga aagtcctaca aagatgtgat agattcactg
     1441 gcagacaaat cgatgctgcc atatcacccg accatttatc ccattccgtt catgctgtca
     1501 agaagataca agacaggaaa ggaattggcc agggctctct tttatagtct ctcctatcct
     1561 gatgtcttgg aatttacaaa gttctccatt tacatagtgg taagcgtttt tgaaaagttc
     1621 cctgatcatc ttgttggctt gatcggtcac gaaatcgcgc acgtaatcgc tgcgaaaggc
     1681 gaagtgagat tgagtgaaga agacctgcgc cttatcctga ggagctggct agacttcatc
     1741 gaggccaagg agagaactgc ctcccgcatc tattcctatt tccgtaaacc gactaaatcg
     1801 atgatcgaga tgtggaatgt ggtcgcgctg cagccagaaa cggaacgtgc tatagcagaa
     1861 ggaatacagc tcgtggacaa ggaacaattt gataagctca tttttggaga acgaattgcc
     1921 gactttaacc ggtttatcga actcaatctg gacagaatca ggggaagtgg gagcccttcc
     1981 tgactacgcg gatagctgat gcttcgcagt acaaagaagc cctgaagtgc agcgccttaa
     2041 gtccaagctt cagcaattga accctttaca tgcagcttct aatcttacta actcgcctcc
     2101 tggttgcatt gtgtctaaat cttgaagctt tttctctcga aaccctctgg gccgatgttc
     2161 tttctaatca atcacacttc attttgtctg ccgttgtcat ttacgtcaag ggcagtccac
     2221 aaaaatagtg atcttagatc atgcatacat gaccctgaca tatctaagct ccatgacagg
     2281 ttgcaaccct tgaagacact tacagcaaag cccagattaa atcaaagggt accaaggatt
     2341 gctcgaaatt cttgaagcac aacatggaag cacatacaaa gcccatcaat tcctaaagga
     2401 agtacaaatg tatttcctga aaacaggcgc catgacaagc ccctgcaagc aatttagctg
     2461 attcccgtag tcacagcgac gggcatttgt ttcctcagcc agagcggtaa tttgagctca
     2521 ttctcacggt accacgtgaa aggatcagct ttgagtcctg acaccatttt tggcagtgtg
     2581 tctagcaagg agtgctgggg attccaaccc aacaaagtgc gtgcgcgggt gatgtcgagt
     2641 tccatattgt cctgggcgac atcgatcatc caaggtgcaa ttgacgccga tcttccgagt
     2701 gccaaaaact tcaagagccg taaaccggtc ttggccacca gcgggggaat tgaccgcgta
     2761 ttccatttta caccgtggat tagttgtcca aaggcgcgct ggaggtcatc atagctgagg
     2821 gacttggtct cgccgatgag cagtacgact tccggcgata gttccttgcg gagtgcgaag
     2881 gcaagcaaga ttgcgtccgc cacatcatcg ttatgcacgt agggatgcct accggtagaa
     2941 gggtcccctg gggagaaata ggctacaggg tctcgctcgt agatgcgttg tatctgcagc
     3001 gccaatggta tcgaatgaca caggtcatcg tatacgcctg cgatgcgcag aaccacgacg
     3061 gggatgttcc cacgttctgc atggatcacg ttctcagatt cgatctttga ctttggatat
     3121 gcccacttgg atggaatatc aagcggcgaa tcctcgttaa ttcgcacccc ttgcctggac
     3181 ggagcgtgta ccaggatggt gctggagaag atgaactgtt cgaccttgaa atcctgaagc
     3241 agccgaagta agcgcctggt acctccaaca gtaactttat catagagagg actcggcgca
     3301 cctgaaaaat cataataagc agcaagatga attacagagg ctatgcgatc accgtgcagg
     3361 tcacggatgg cttgcagccc gcggcgcgta ctttcttcat cagttagatc cacgtacaag
     3421 cattcagcgc ttggtaaagg atgcgatggt accttgcggt caaagccgat aacgttgaag
     3481 gattgttggt cctcagccaa cctctttgct atagcgtaac caatcctacc actggtgccg
     3541 gtgaccaaga tcgtttcttc tttcgcttca ctcatctatg atcttctcca tttctcattg
     3601 ataataatca tcattatcaa aatcattatt tttggcctgt ccagacatta ggttcttttt
     3661 ttgctgcatg ataccagacc tcttaaactt ctatgattaa atgagtttac atttactaat
     3721 gtaaagcgga ttagtctaag caattcttgc c
//
LOCUS       DASQTF010000232         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_914736,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000232 DASQTF010000000
VERSION     DASQTF010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914736"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VE308_05500"
     CDS             complement(<1..855)
                     /locus_tag="VE308_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HZB00458.1"
                     /translation="MLSIWLRAIRIRFLLASVIAVSNGLAIAYWKYANIDPLYALLTY
                     VGVVFLHASVDLLNDYWDHKRGIDSVTKRTKFSGGTGVLPENLLTPRAVYIAGIIFLI
                     LGASIGAYFVAIRGITIAVILGFAVVAIYFYSTRIVNAGLGELFVAIKGAMIVLGTFY
                     VQNAVLAPDALYVGVIVGILSATVLFINSFPDYEADRSKGRRTLVIMLGRKTASALFP
                     IFIIAVYAMIAGGILLGFTKVYSLISFVSIPLAIKSILSLRVESENVEKMVPAMASAV
                     TYSRITGFL"
     gene            complement(903..1166)
                     /locus_tag="VE308_05505"
     CDS             complement(903..1166)
                     /locus_tag="VE308_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00459.1"
                     /translation="MPIDAAEFDAHKDVGTIITQFLCRNSDKGYSAREIAQATGIAEA
                     NVNNAMLKLGLSDLASALAGKRKKFRIEDVTLNGITYYRCVSQ"
     gene            complement(1197..1598)
                     /locus_tag="VE308_05510"
     CDS             complement(1197..1598)
                     /locus_tag="VE308_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HZB00460.1"
                     /translation="MFTLYTPQTADKALPEVKRLFENIITLKNHVVALQEEIQAIIDS
                     GSPFEGFIKKKQELNTSVTTLYKAIEQLEETGVMIKSVDEGLLDFPSRRFDEEVWLCW
                     KVGENKIKFWHGKDEGFMGRKPLESTGVIEP"
     gene            1986..2189
                     /locus_tag="VE308_05515"
     CDS             1986..2189
                     /locus_tag="VE308_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00461.1"
                     /translation="MVEPTLDTEKVMPTSMKGKEEHENLVPFLEQEIECPRCHDSMML
                     CSDFDNLYYVCEECDFCLYTIKK"
     gene            2257..>3020
                     /locus_tag="VE308_05520"
     CDS             2257..>3020
                     /locus_tag="VE308_05520"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HZB00462.1"
                     /translation="METFHRTRSSRLILSTLAVAIDSVRPAPLIKRAVKFSNNELIVR
                     DIRGHVSKLREFDRVYIVGAGKAAPGMAYTLCSILGNKVGAGAITVPYGTKAEIKRVL
                     VTEASHPIPDKAGIKGTRNILSILRKADQNDLVIFLISGGGSALMPMPASGISLTDKQ
                     KITSSLLRSGASIHEINAVRKHLSAVKGGQLLRHVNESCSLLSLILSDVIGDDLGMIA
                     SGPTCPDNSTFNNALNILKKYRIESPVTAIRYVAKGA"
BASE COUNT          864 a          683 c          617 g          856 t
ORIGIN      
        1 caaaaagcca gtaatcctag agtatgtcac agctgaagcc attgcaggca ccatcttttc
       61 cacattttcc gattcaactc taagtgacaa gattgatttt atagcaagcg gtatcgacac
      121 aaaactgatt agagaataga ccttggtaaa gccaagcaaa atacctccgg cgatcatggc
      181 ataaacagca atgataaata tggggaatag agctgaagca gttttccttc cgagcattat
      241 cactagggta cggcgtcctt ttgacctatc tgcttcatag tccggaaatg aattgataaa
      301 gagcactgtt gcggacagaa tgccaactat taccccgaca tatagagcgt ctggcgctaa
      361 taccgcattt tggacgtaaa acgttccaag cacgatcatc gcccccttga tagcaacaaa
      421 cagctccccc aagccagcat ttacaattct tgttgaataa aagtagatcg caacgactgc
      481 aaagccaagt attactgcta tcgttatccc gcgtattgcg acaaaatacg cccctatcga
      541 cgcgccaaga atcagaaata ttatgccagc tatgtaaact gcgcgaggtg tcagcaggtt
      601 ttcaggtaga actcctgtcc ctccactgaa ctttgtcctt ttcgttacac tatctattcc
      661 tcgcttatga tcccagtagt catttagaag gtcgacgctt gcatgtaaaa aaactacgcc
      721 cacataggtg agaagggcat aaagtgggtc tatgttagcg tatttccagt aagcaatcgc
      781 aaggccatta ctgactgcaa ttactgacgc taggagaaat ctgatccgga tagctcgtag
      841 ccaaatagag agcacttatc tcctgccata tgcagaggag cttattacta tgttttttga
      901 aatcattgag aaacacaccg gtaataggtt ataccattta acgttacgtc ctctatacga
      961 aactttttcc tcttgcctgc taaagcactt gcaagatctg ataatcccag cttgagcatg
     1021 gcattattta catttgcctc tgcaataccg gttgcctgtg ctatctctct agcagaatag
     1081 cctttgtccg aatttctaca taagaattgc gtaatgattg ttccaacgtc tttatgggcg
     1141 tcaaactctg cagcatctat aggcatatca aaccgccacc aatagattct ataaagctac
     1201 ggctctatta cgcctgtgct ttcaagcggc ttgcgcccca taaagccctc atcctttcca
     1261 tgccaaaact ttatcttgtt ttcacctact ttccagcaca gccagacctc ttcgtcaaac
     1321 ctccttgaag ggaagtcaag caagccttca tccacgcttt ttatcattac tcctgtctcc
     1381 tcaagctgct ctatcgcttt gtacagagtg gtgacagaag tattaagctc ttgttttttc
     1441 tttatgaaac cttcaaaagg actaccagag tcaattattg cctgaatctc ctcttgcagg
     1501 gcaacaacat ggttctttag cgttatgata ttttcaaata aacgcttgac ctccggcaat
     1561 gccttatcgg cagtctgtgg cgtatataac gtaaacatta atgagatttt gttcaatttt
     1621 gagataattt atgcttttag cacctgacgg gagaagagca tacaattttt gagaatcctg
     1681 ttatgtcatt tttcataatg gacctattgt aatctctcca ttcttaatgt cgaggagaat
     1741 aagggaatac aaatgctcca agtcaaactt tatcagacaa aaaagagaaa ttaaccggcg
     1801 atgtctgtaa aaaatcaaaa atagaaggat agacgaatta ggccttgatc tttgccgaca
     1861 aaagcgcttt attgcttgta aggcttgtat gcatttttta tcattccaga ggaataggga
     1921 tacctctccc tccctctctt cgaggtgttg ctggctttaa aaaggactgt ctattacttc
     1981 tttttatggt agaacctacg cttgacacag aaaaggtaat gccaacaagc atgaaaggca
     2041 aggaagagca tgaaaatctg gttccgttct tggagcagga gatagaatgt ccccgttgtc
     2101 acgacagcat gatgctttgt tcagattttg ataacctgta ttatgtctgc gaagaatgcg
     2161 acttttgcct gtatacaata aagaaatagg cattataaaa agaaattatt aggtgctgta
     2221 actggtagtt aatgattgat tgtaaatcgt catcaattgg agacatttca caggactagg
     2281 tcttcgcggc taattctttc tacacttgca gttgcaattg acagcgtgag acctgctccg
     2341 ctcataaagc gtgctgtaaa attcagcaat aacgagctga tagtacgtga cattcgcgga
     2401 catgtctcaa aactgcgtga atttgaccgt gtatacattg tcggcgccgg taaagcagca
     2461 ccaggcatgg cctatacgct ttgctctatc ttgggtaaca aggttggtgc tggcgcgatt
     2521 accgttcctt atgggaccaa agccgagatc aagagagttt tggttactga ggcctcgcat
     2581 ccgatccctg acaaggcagg gataaagggc acaagaaata tcctctcgat cctgagaaag
     2641 gctgatcaga atgatcttgt aattttccta atctcaggtg gtggctcggc gctcatgcct
     2701 atgcctgcat caggcatctc tttgactgac aagcaaaaaa tcacaagctc gctccttcgc
     2761 tctggggcat caattcacga gataaatgcc gttagaaagc acttatcagc ggttaaggga
     2821 ggtcagctcc tgcgacatgt gaacgaatca tgcagccttc tgtcgttgat cctctctgac
     2881 gtgataggag atgaccttgg aatgattgct tctgggccta cctgtcctga taactcgact
     2941 tttaacaacg ccttgaatat cttaaaaaaa tatcgcatag aaagtcccgt tactgctatt
     3001 cgatacgttg ccaaaggagc
//
LOCUS       DASQTF010000233         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_918042,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000233 DASQTF010000000
VERSION     DASQTF010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918042"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            822..1769
                     /locus_tag="VE308_05525"
     CDS             822..1769
                     /locus_tag="VE308_05525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZB00463.1"
                     /translation="MKSAQIKRYGSSEVVEINQSTPAPNDPSAGKVLVRVKAASVNPA
                     DWKIREGYMQQMMPLQFPSTLGMDFSGVIEKVGEGVSDSEQNDEVYGQASVISGGSGA
                     FAEMALANADSIAHKPETLSHDEAAGLPLVGVSTWQALVETIRLSKGQKILIHGGAGG
                     IGSIAIQLAKNLGAYVATTSSTNDKQFVQQLGADEVIDYKTQTFEDLLHDYDAVFDTI
                     GGETYTRSFKVLKRGGGIIVSMLEQPNQELMDRFGVKAISQFTQVNRERLTKLAQWVD
                     QNNIRVNVDKTFTLDEASKALDYQRDVHPRGKVVLQVSK"
     gene            complement(2296..>2630)
                     /locus_tag="VE308_05530"
     CDS             complement(2296..>2630)
                     /locus_tag="VE308_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00464.1"
                     /translation="LDPAFAIPAGVGDILIGVTAIPFAIFLWRGYSWSKYAVVVWSVL
                     GIADLINAVSLGLIINSDFSTSTMATFPWILVPTVGVPLALALHGIMLYRLRKWVPIQ
                     AHRKLPET"
BASE COUNT          818 a          517 c          599 g          696 t
ORIGIN      
        1 ggttgtcctc atgactgctt ttgagataag agatagtgag ttttctaaag tatttccttc
       61 tacgcatgtt gacggctttg ttcaaaaacc aatccgtatt ggtgatctga ctaacaaaat
      121 attgagtgtt ataggcgaaa cgaaatgaag gatggattag gaattctgaa tagcgaaggt
      181 agtaaatgaa tcatcgaata ttgcagtggc agaaagcatt gctagcagta tttttgtacg
      241 acaacctgct agaataagct caattcaatg cagtagccta ctagattgat aaaaatggac
      301 aggggggatt ggaacccaag acctctatcc atgagcaggt ttccatgctg cttctggaac
      361 tacacactat tcactggagt tgttgttgaa agagaactca tttctcaaat cccacccact
      421 ccactttttc ttttttcatg atctccatag ccctatacag tcgagcgaag ttgttttgag
      481 atttagctag ctaaggtagt aaacaagata aaactatggc aggttttggt cgtttggcag
      541 agaaagggaa aatggaagtt gctccttaca ccctggggtt ttagtataat acaacaacaa
      601 ctcctagcat aagcaaacca ttcatacaag agtttcatat ctatcagttt tccaattgtt
      661 gctcccagtg tttcagatga gaactctaac cagcgtttgg aattttcatt acaagacgag
      721 gatttgggaa aagcgatcgg gtacggttct tgttgttacc gacttagtga tatcttacta
      781 accaccaatt ctttaatatc tcatactgcc ttgtatcatc aatgaaatcg gctcagataa
      841 agaggtatgg aagtagcgaa gtagtcgaaa tcaaccaaag tacgcctgca ccaaatgacc
      901 catctgcagg taaggttcta gttagagtaa aagcagctag tgtgaaccct gcggattgga
      961 agatacgaga aggttacatg caacaaatga tgccactcca atttccgtca accttaggaa
     1021 tggatttttc aggtgtcatc gaaaaggtcg gggaaggtgt ctctgattct gagcagaatg
     1081 acgaggtata tggacaggct tcagtaataa gtggaggttc aggagcattt gcagaaatgg
     1141 ctttggcaaa tgcagacagt atagcacata aaccagaaac tttaagccat gatgaggctg
     1201 ctggtttacc gcttgtaggc gtgagcactt ggcaggcgct tgtagaaact attaggttat
     1261 ctaagggtca aaaaatcctg attcatggcg gcgcaggagg cataggaagt attgctatcc
     1321 agttagctaa gaatcttggc gcatatgtag caacgacgtc aagtacaaac gataaacagt
     1381 ttgtccaaca gctgggagcc gacgaagtaa tagactacaa gactcagact ttcgaggact
     1441 tgctgcatga ttatgatgca gtctttgata ccataggcgg tgagacatac acaaggtctt
     1501 tcaaagtatt gaaaagaggt ggtgggatta ttgtatcgat gctagagcag ccaaatcaag
     1561 agcttatgga tcgctttggt gtaaaagcca tctcacaatt cacacaagta aacagggaac
     1621 gattgactaa attggcgcag tgggtcgatc aaaacaatat acgtgtaaat gtggacaaga
     1681 cgtttacact agatgaggct agtaaggcgc ttgactacca gagagacgta catccaagag
     1741 gcaaagtagt gttacaagtg agcaagtaaa atgagtttga aggaaaatga atcaattggg
     1801 tggtgactca tgaaaactac aaccattaag aatgagctca aaataatgcc gcagtttcct
     1861 cgtagtaaga aatggaccgg gtgggagttg aacccacgac ctcagctctc tatagctatc
     1921 tataaggtca gccgatgagg agaattgaac tgctcaaatc ccacccggtc tactgacatg
     1981 ctcaagattg aattgttttt ggcgagcaga ataatacacg actcgccctc tttcaatgat
     2041 aatagctaac gcccattttc tttcttccat ttttacagcc gccatcccat cctttactga
     2101 tgatgaagtg tgatgatggc tacaacaaat attagctagc actgaagaga tgtattaagg
     2161 cattatcata gtaggagatt cgcataagag ggcagtaaag ttcgaagttg cctgttaact
     2221 acaggcacaa tgcaagaaaa agatgagagg acgaagactt cgtaatagtc ctttgtccta
     2281 ttttgcatac tcgcatcaag tctctggaag tttcctgtgt gcttgtattg gtacccattt
     2341 ccttagtctg tacaacatga tcccatgcaa ggctagcgct aacggtacac cgacggtagg
     2401 aaccaaaatc cacgggaatg ttgccattgt ggaagtgctg aagtctgaat tgatgatgag
     2461 accaagactt actgcgttga tcagatcagc aatacctagc acactccaca ctactactgc
     2521 atactttgac caactatacc ccctccagag gaaaatcgca aaagggatag ccgtcactcc
     2581 tatgagaata tctccaactc ctgcaggtat cgcaaaagct ggatctagga
//
LOCUS       DASQTF010000234         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_918571,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000234 DASQTF010000000
VERSION     DASQTF010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918571"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            677..>2189
                     /locus_tag="VE308_05535"
     CDS             677..>2189
                     /locus_tag="VE308_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZB00465.1"
                     /translation="MAVILQSLPHEASLTKIEYEGPCIALYSKNPSYLLKNNQLVLNM
                     VNSIKKRIVIRTDESLRLSQQESIKVIADTIPRDAGITGTFFDAALGEAVIFAKKPWT
                     ILHEGGDFDLTEKIGWKVRVRKAPQNILPIETLYKTLGETVSQRIKFYREVGDKIFRA
                     KLTEQAEASLLTLGGFAEIGRSCMLLETRESKIILDCGLNMSAKDRLAALPRFDIAGI
                     GMEDVDAVVLSHAHLDHTGFLPALFKYGYSGPVYCTEPTLLLMSMLQRDYLTHSGNDA
                     LYSEQDIDSAVTHAITLMHGIVTDISPDVKLVLSNSGHIIGSTSIHLHIGNGQHNLVY
                     TSDMKFGKTLTLENASWNFPRVETMIIESTYGGKEDIMCPREQAEANLASAVERTAAA
                     GGHVLLPVPAVGISQELILVLDKFTKGGRLQTRVLVEKIISETTAVYEAYPEFLSKEL
                     GKYVLESETSQFGSQFSIVESETLDPSQPAVVLAPSSMLIGGPSVGYLKQIAND"
BASE COUNT          629 a          488 c          484 g          588 t
ORIGIN      
        1 ggcaactcca tcaggtgggt cggcggagaa ctcaattaga aaaaccctgc acgcggcaat
       61 taattaagct tgcacctatt tgatttctcc tcatgcttta gcgaatttac agtgcagagt
      121 tttggcacct gtctagcttc aattacacca gctgatgtgc ttctgctcct tatctcaata
      181 ttcacctcac cggttctgca ttgataatgc ttatctttgt taaatgtatc tgatatcgct
      241 gatagccata ttaccttcct cagatcgacc tgcatcttag aactttattc tgtatctgtt
      301 gattatctat cagacagtct cggtcgaagc gcattggcaa tgattgaaca aggttggatt
      361 ttcgaaccta tttgctagac taatactatt gccctcttat caacatctaa tgacttttag
      421 ctctctaccg tagtgatact tcagtaggaa atgagtagca tgagctatat cttgcgaatt
      481 gaatgcaatg caacttaaac tggtatagta tttttttaat tcttaacttg agacaaacaa
      541 gccaaatact taatacaggc atcaatgtaa aatatgccat tactcttgac ggagacgtta
      601 gtttgacggt catctaggaa ttacattcat gcaaagtaaa tcgccctacc atcatgaatc
      661 tactagccaa aatattatgg cagtaatact gcagagtctg ccgcacgagg ctagcctgac
      721 taaaattgaa tatgaaggcc cttgcattgc actttattct aagaacccaa gctacctact
      781 aaagaacaat cagcttgtcc taaacatggt caatagcata aaaaagcgga tcgttatccg
      841 gacagatgag tccctaaggc tttctcaaca agagtccata aaggttattg cagataccat
      901 tccaagagac gctggcatta ccggcacatt ctttgacgcc gctttaggcg aggcagtaat
      961 ttttgctaag aagccgtgga ccattctgca cgaaggtgga gattttgatc ttacagaaaa
     1021 aataggctgg aaagtcaggg tcagaaaagc tccccaaaac atactgccaa ttgaaacact
     1081 ttacaaaaca ctgggtgaaa cagtgagcca acgcattaag ttctatcgag aagtggggga
     1141 taagatattt cgtgctaaat tgactgagca ggccgaagcg agccttctca cgctaggtgg
     1201 atttgcggag ataggcaggt catgtatgct acttgaaacg cgtgaaagca agattattct
     1261 cgactgtgga ctcaacatgt cggccaagga ccgactagca gctttgcctc gctttgacat
     1321 tgcaggaata ggtatggaag atgtagatgc agtggttctc agtcatgcac atcttgatca
     1381 taccggcttt ctcccggcgc tcttcaaata cgggtatagc ggtccggtct actgcacaga
     1441 gccaactctc ttgctcatga gtatgcttca gcgcgactat cttacgcatt ctgggaatga
     1501 cgcgttatat tcggagcaag acatcgacag cgctgtaaca cacgcgataa cactgatgca
     1561 cggtatagta actgatattt cgccagatgt caagctggtt ctttcaaact cgggacacat
     1621 cataggatcg acttcaattc atctgcacat tgggaacgga cagcacaatc ttgtatacac
     1681 aagtgatatg aagttcggca agacacttac actcgaaaac gcttcttgga attttcctcg
     1741 agttgaaaca atgataattg aaagcaccta cggcggcaag gaggatatca tgtgcccgcg
     1801 tgagcaggcc gaggccaatc ttgccagtgc agttgaaagg acagctgcgg caggcggtca
     1861 cgtactattg ccggtgccgg cagtgggcat ttctcaagag ttgattctag tacttgacaa
     1921 gtttacaaaa ggcggcaggc ttcagaccag agtgcttgtg gaaaagataa tctcagaaac
     1981 aacagcagtc tatgaggcgt atccggagtt tctttcaaaa gaacttggca aatatgtgct
     2041 cgagtcggaa acaagccagt ttggctcaca gttctcaata gttgaatcag agactcttga
     2101 tcctagccag cctgcagtag tccttgctcc ctcatccatg ctcatcggag ggccatctgt
     2161 gggatacttg aagcaaatag cgaatgatc
//
LOCUS       DASQTF010000235         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrososphaera sp. isolate SMAG_U10928 k141_923338,
            whole genome shotgun sequence.
ACCESSION   DASQTF010000235 DASQTF010000000
VERSION     DASQTF010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946405
            Sequence Read Archive: SRR5918993
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaera sp. (soil metagenome)
  ORGANISM  Nitrososphaera sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Nitrososphaera.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:47:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,114
            CDSs (total)                      :: 1,091
            Genes (coding)                    :: 1,089
            CDSs (with protein)               :: 1,089
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Nitrososphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923338"
                     /isolate="SMAG_U10928"
                     /isolation_source="soil"
                     /db_xref="taxon:1971748"
                     /environmental_sample
                     /lat_lon="36.67146 S 142.28934 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VE308_05540"
     CDS             complement(<1..291)
                     /locus_tag="VE308_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00466.1"
                     /translation="MASTGGVEARGFLCIPHDKQSGSLLENWKKSDNLVDIYFVTATF
                     SEESTPYFPNKANHYILASFIDVTGMLDDIKNHKMDRLSLIFSFSNPLFERSG"
     gene            737..1057
                     /locus_tag="VE308_05545"
     CDS             737..1057
                     /locus_tag="VE308_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00467.1"
                     /translation="MTSVSFVLFRAKSPAGNSVSDRHKPELAVYHLTEKFDLEAESTK
                     TFCGKLLPINWGVNFLMITKDETQRIQEAAENPEFCRKCVKVAIVINDIEERPERRRR
                     RKRH"
     gene            1154..1330
                     /locus_tag="VE308_05550"
     CDS             1154..1330
                     /locus_tag="VE308_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00468.1"
                     /translation="MLSLQQVIDERIFFLKEQINHTKNKPEVINTFQLQIDAIRSVDD
                     IEKVEFIFCKRRFY"
     gene            2281..2403
                     /locus_tag="VE308_05555"
     CDS             2281..2403
                     /locus_tag="VE308_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00469.1"
                     /translation="MQLGLFVVLTTIIMMMMMRRRRRRRRMTTVIPKTKRTAAI"
     gene            complement(2495..3457)
                     /locus_tag="VE308_05560"
     CDS             complement(2495..3457)
                     /locus_tag="VE308_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZB00470.1"
                     /translation="MSEVKRQFETITSNGVLWVNIQKPTFADINALGQKYPFHRLNLE
                     DCLSKIQIPKIDRYESHIFAILHFPTPDKEKGFLFSQLAIFMGSDYLVTIHQGDLKAL
                     QELFYMCKKDEKQQQAIMGRSSGYLLHKIIDTLVDDLLHILMKVVGNIDDIEDSVFDE
                     QISIPRQISLLRREITTLRRIVIPLRRTVVDLSKDVQRFSREDLAPYFKDVQDHIEKV
                     YEALEEAKETVEIYKDTDYMLSQEKTNKILGVLTIVFTLSIPATVIGTFYGMNIVLPG
                     GSETEPWTRLGPYTTLIFVLVVSSISAILMILYFRRLGWLGSSR"
     gene            complement(3520..4413)
                     /locus_tag="VE308_05565"
     CDS             complement(3520..4413)
                     /locus_tag="VE308_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZB00471.1"
                     /translation="MRSITVGIPSYNERQNIVNLLRAIEEQPTPIFEVLISDDSSDNT
                     PSLIRNFAQHSMLDIQLFHHDTRRGAAAAWNEILQRAAGDIVVLYDADTIPHPSCTAQ
                     LASQIQGNVGICASNSQPVQAAGIAGRASVFISNWLRSVRLARLSQYTVMGRALAISS
                     REAKKIEIPTKIIAIDLYIQCKILEMGLNIIYNDNAIVYFKPADKIDDLTSQVMRAVN
                     GHKQIEDYVLSCGIGLPRQVAIANAVKNAAKDPLGAMSVAIGYSLISYYRSRLEHTDT
                     AKWHTATSSKAIDYQQLKTRF"
     gene            4523..>5085
                     /locus_tag="VE308_05570"
     CDS             4523..>5085
                     /locus_tag="VE308_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZB00472.1"
                     /translation="MQRQNTCSDEEGYKAIFASSGLRKSHLIFPVFVSDSPSMIKSMP
                     GMAVTPVQRISQHVQSIADSGISSVIIFGIPKSRDQIGSSAFDRKGIVQDAIREIKSS
                     FGSAMSVATDVCICQYNLTGHCGIASKGGRVNNDATLKVLAKIAASHAEAGADVVAPS
                     AMMDGQVYAIKRELDSRRYGASILSFSA"
BASE COUNT         1427 a         1059 c         1118 g         1481 t
ORIGIN      
        1 accacttcgc tcaaagaggg ggttggagaa actgaatatg agcgataacc tgtccatttt
       61 gtggtttttg atatcatcca gcataccggt tacgtctatg aaggatgcga gaatgtagtg
      121 attagcctta ttggggaaat atggtgtact ttcttcagag aacgttgcag taacaaagta
      181 gatgtctaca agattatcgc tttttttcca attttcaagt agggagcctg attgcttatc
      241 gtgagggatg cacaaaaagc ctcgcgcctc cacgccccct gtgctcgcca atcttacaca
      301 caagtaatgt aactactatt tatctattaa tgctgatttt ggtctgataa tacaactctc
      361 actcactaaa tcgtaaacaa acaagaacaa gacagacatt ctacatacaa caggtataat
      421 gaaagtaaga gaagaacaac agaaagagtc tatgtgtttt cagtagagag aggctgtatc
      481 tagctgctac tccgctgcgg ctgcgtcttc tcctccttct tcctcacctg ctgccatagg
      541 tgtcagcagt agcatttgtc gtcgtagtca tcatagccat aagcaatgac actgtctctt
      601 ttattccctt ttttgggaat ttttttattg caatcctttt acattagacc ttcgtaacta
      661 attacgaccg gctaaatgga tagactaagg aatttgactc gggaggtgaa caggcgtgga
      721 ggcagttccg agagcgatga cttctgttag ctttgttcta tttagagcaa agagtcctgc
      781 gggtaattct gtctcagata gacacaagcc ggaattggcg gtttatcatc tgactgagaa
      841 attcgaccta gaggcagaaa gcactaaaac attttgtgga aaactgctcc ctatcaattg
      901 gggcgtgaac tttctaatga taaccaagga tgaaacacaa agaattcaag aagcagcaga
      961 gaatcctgaa ttttgtcgaa agtgtgtaaa ggtcgcaata gtgattaacg atatagaaga
     1021 aagaccggaa agaagaagaa gaagaaagag acactagtcc aacaaaaaca tgataatgat
     1081 aatacccctc ccctaaggtg ctagccatga aataaccatc cgtctggggc ttgacttaac
     1141 tagatataac gtgatgttga gtctacaaca agtaatcgac gaaagaatat ttttcctaaa
     1201 agaacaaatt aaccacacca agaacaagcc agaagtgatc aacaccttcc aactgcagat
     1261 agacgctatt agatctgtcg acgatattga aaaagttgaa ttcatattct gcaaaagaag
     1321 gttctattaa agaattgtat agatgtgtac gaatccgata ggttctttgc ggagcttgac
     1381 ggtttggaat ggcagctgca gcggcagatt gcaaggtact cgaagaagaa ggataaggag
     1441 cagcgaccta ctacatcatg ttgctattgc agcatatgcg gcaagaaatt aaaattcgct
     1501 agaacaaatg aaaacccatt gtcatgccgg aagtgcatga tactcaacat ggattcctga
     1561 aaatgctcat cgatgtggta gtagtgatag tattttatat atcataagcg atgtagtggt
     1621 ttgtatcgta aatctacata gtgtataata gtctaagcaa gaatcagtca gccaaaaagc
     1681 tcttttagca tttgtctacc tgatggcgtg atgatttttt cggtcataac ctgtagactt
     1741 tttcgaagaa cttaaggagg cagcacgttt ggttattcgt gacgctagta cctttgaatt
     1801 cgttattcta tgattcttat ctatgtgatc ttttagttta ctctttgaat taaagaattt
     1861 accgcagctg ttgcatcgtg aaattttgcc cgtcatgtct ttctttgtgt gtccccttaa
     1921 taggggattt cttcactatt taacctttga tggtaaattc gttcctgact tattagaatg
     1981 catttgaaca caaaaaaact cgcaccgctt gcatcaatgt gatagtatgg ctagaagaac
     2041 acatgataat cattttttct tctcctcatc cctagtatga tgcttcacgt aaaaatcaag
     2101 cagcatctat atgatttggg cgtaggtgga atcaaagcca ccatgggatg ctaacctttt
     2161 tgccagttca tcccacactt taatgttctc ggtcatgccc gattgctgtt gttgagctaa
     2221 catatacgag ctgactatcg gtattactga gctttaaagg taatcacaga ttagctgcaa
     2281 atgcaactgg ggttgtttgt cgtattgacg acaataataa tgatgatgat gatgaggagg
     2341 aggaggagga ggaggaggat gacgactgtg attccgaaga cgaagaggac tgctgcaatc
     2401 taacggtttt tagctattgg ggtgctacta aagggacatg gttgtcccta tactttatct
     2461 attaggtaaa tagcaattgt caaatacgcc atggttacct tgatgatcct aaccatccaa
     2521 gccttctgaa atataatatc ataagtatgg cagaaatcga cgaaaccacc aaaacaaata
     2581 tcaaagtagt gtaaggcccc agccgagtcc aaggctctgt ttctgaccca cctggcagaa
     2641 ctatgttcat gccataaaag gtgccaatga ctgtagctgg gatcgatagt gtgaacacta
     2701 ttgttagcac gcctaatatt ttgttggttt tttcttggct gagcatgtag tccgtgtcct
     2761 tgtagatctc tactgtttct tttgcctcct ccaaagcttc gtacaccttt tcaatgtgat
     2821 cttgaacgtc tttaaaatat ggagcaagat cttctctcga aaagcgctgc acatccttag
     2881 acaggtcaac taccgtcctg cgaagtggga ttactattct tcttaaggtt gttatttccc
     2941 ttcttagaag agagatctgg cgtgggatcg atatttgctc gtcaaatacg ctatcctcaa
     3001 tatcgtcgat gttgcctaca actttcatca gaatgtggag gaggtcatcc accagtgtat
     3061 cgattatctt atgaagtaga taccctgacg acctgcccat tattgcctgc tgctgctttt
     3121 cgtccttctt gcacatgtag aatagctctt gaagagcctt caggtctcct tggtggattg
     3181 tcaccagata atcggagccc ataaagatcg caagctgact aaagaggaaa cccttttcct
     3241 tgtcgggtgt aggaaaatgc agaatggcaa agatgtgact ttcataccgg tcaatcttag
     3301 gaatctggat cttggagagg caatcttcaa ggttgagcct atggaacggg tacttctgcc
     3361 caagagcgtt gatatccgca aatgttggct tttgtatatt tacccacagc accccgttac
     3421 tggtgattgt ctcaaactgc ctcttcacct cagacatccc gaaacccttc tcgcttgttt
     3481 cgttacagaa gatgggttat aaaaaagtgt ttcagtcagt cagaaccttg tctttagttg
     3541 ctgatagtct attgctttcg agcttgttgc agtatgccac tttgctgtgt cagtgtgctc
     3601 aagtcttgac ctgtagtacg atatcaacga atatccgatt gcgactgaca tggcaccaag
     3661 cggatcttta gcagcattct ttaccgcatt ggcaatcgct acttgtcgcg gcaaaccaat
     3721 gccacatgat aagacgtaat cctctatctg cttatgtcca ttgactgccc tcatgacctg
     3781 cgatgtgaga tcgtctattt tatcagcagg tttgaagtac actattgcat tgtcattgta
     3841 gattatattt aggcccattt caagaatctt gcactgtata taaagatcaa ttgcaatgat
     3901 cttcgttggt atctctattt ttttagcttc cctagaacta atggcaagcg ccctgcccat
     3961 caccgtgtac tgagacagcc gtgctagcct cacggacctg agccagtttg agatgaatac
     4021 agacgcccta ccagctatgc ctgccgcctg tacgggctga gagtttgatg cgcagatacc
     4081 aacatttcct tgaatctgcg acgcaagctg cgcggtgcag gaagggtgag gaatggtgtc
     4141 agcatcataa agaactacga tgtctccagc cgccctctga agaatttcat tccatgccgc
     4201 ggcagcaccc cttcttgtat catggtggaa aagctggatg tcaagcattg aatgctgggc
     4261 gaaattgcgt ataagactag gtgtattgtc agatgaatca tctgaaataa ggacttcaaa
     4321 tatcggtgtt ggctgctctt caattgcccg taagaggttc actatgtttt gcctttcatt
     4381 gtatgaagga ataccaacgg ttatactgcg cacattagct tcgtctccta gataccattt
     4441 gagttttttg attgattatt ggtgtgtttg ctttgtgaaa acatatattc ataggctgct
     4501 cgccaagcag tgaggatacc ttttgcaacg gcaaaatact tgcagcgacg aggaaggtta
     4561 caaggcaata tttgcctctt cgggcctacg caaaagccac ctcatatttc ccgtatttgt
     4621 ttctgacagt ccaagcatga taaaatcaat gcctggcatg gccgtgacgc ctgttcaaag
     4681 gatctcacag catgtccaat caattgctga tagtggtatt tcatcagtga ttatctttgg
     4741 catacccaag agcagggatc agattggctc atctgctttt gacagaaaag gaattgtcca
     4801 ggacgcaata agggaaatca agtcatcttt tggcagtgcg atgagtgttg caactgatgt
     4861 ttgcatttgc cagtacaatc tgacaggaca ctgcggcatc gccagcaaag gcggcagggt
     4921 aaataatgac gctactctaa aagtcctagc caagatcgcg gcaagtcatg cagaagccgg
     4981 cgcagatgta gttgctcctt ctgccatgat ggacgggcag gtttatgcaa tcaagagaga
     5041 gcttgatagc cgcaggtatg gtgcatcaat actctccttc tctgc
//
