LOCUS       DASQUQ010000001         7306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1171280, whole genome shotgun sequence.
ACCESSION   DASQUQ010000001 DASQUQ010000000
VERSION     DASQUQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171280"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VE486_00005"
     CDS             <1..459
                     /locus_tag="VE486_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZA37497.1"
                     /translation="VSDPMIKHQIRIAAEKEEKEFYEHRAPKAWLEALAPIGTDSRKP
                     FLETAPWLIAVFAQPFRILADGTRSPTYYAIESVGIATGILVTAVHSCGLAALTHTPS
                     PMGFLNRILDRPSHEKPFVLLVVGHPAENALVPDITRKPLDEISSFRSNA"
     gene            536..1225
                     /locus_tag="VE486_00010"
     CDS             536..1225
                     /locus_tag="VE486_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37498.1"
                     /translation="MFVGHYSVAFAAKSERNRIPLWVLFIAVQFLDYIWATLVLLGIE
                     KLRVIKGFTAGSMIDSYYHPYSHSLITALIWSAVAAVIYKSVWRAKASSAAALLVGVA
                     VFSHWVLDFIAHPRDLPIYGNTWKVGLGFWNYRGPEFVLEVALLAGGIVLYQARNAIS
                     TARKKAVIGFGLALVIVQIGDTFVPRTPLSDRATAMGVWVFYTLFVIVAYLLERGAPA
                     RIVNHNSRSRR"
     gene            1369..4008
                     /locus_tag="VE486_00015"
     CDS             1369..4008
                     /locus_tag="VE486_00015"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA37499.1"
                     /translation="MQHRPVGTVTFLFTDMEGSSRAWESYPSETRAALQRHDEIMARE
                     VEGHNGAIILERGEGDSVFAVFTRGNDAVSAAFAIQRAFQEEPWPSHVPVRVRMAIHT
                     GQAATDYRGPDVNRAARIRAIGHGGQILVSDVTAGIVRGTLPEKAALIDLGHHRLRDL
                     PGTEHVFQLAHPELREEFPPLKSLGNFRQNLPVQLTSFIGRESEQKTVRSLINDHRVV
                     TLIGSGGCGKTRLAIQVAADLLEQFPDGVRFVDLAPLSDPSLVLDGIAAAVEVKLEQG
                     DAKDDALVRTLQGTKTLIILDNCEHLVRACADAVSVLLRTGEHVRILATSREPLGLPG
                     ERTWRVPSLSLPDGATSVEEVSACGAIQLFVDRAAAAREGFALSAGNAQTVVDICRTL
                     EGVPLAIELAAVRVKVLSPREIRDRLSDRFRLLTGGRGRHQTLRSAVDWSYDLLSEQE
                     RGLFRRLSVFAGSFDLTAVKAIWPESDPLDRIEQLVDKSLVTVEQLNDDKLRYRLLET
                     LRRYGAERLVEAGEEEDTRERHFGHYLGVAERAYEQRIEDEAASLAALEADHDDFRLA
                     LQWARSRPRDLLRLASALGWFWHLRSYYREGRSWLEEALRLNPAERSGEKARALWALS
                     MILNWQGDVAAARPLAEESLALWRKSNDPLELALALESIGWSHFAGSNYSEALRSMED
                     CVASYRKLGSAKLITRGRVAVGQMLAALGDVERAEQLARETLAEGRAQREPKFVHFSL
                     HYLADCALWRGDAKNAVQLYGESLRAALDYGNEMEAATEMQGMAMGLMGSERQEEGFL
                     LYGASCARFGELHTTMIDDVAFWVKFRERYLPPARERIGAAAADEAEQEGRAMGWQKA
                     LAHAFAVAGEGAIGLNEAKTDTI"
     gene            3983..5104
                     /gene="amrS"
                     /locus_tag="VE486_00020"
     CDS             3983..5104
                     /gene="amrS"
                     /locus_tag="VE486_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZA37500.1"
                     /translation="MRQKLTQSDYPGRWWMMLPDGRIECRLCPRFCKLNDGQRGFCFV
                     RQRLGDRMVLTTYGRSSGFCVDPIEKKPLNHFFPGTSVLSFGTAGCNLGCRFCQNWDI
                     SKAREWDKLADAASPEQIALAAQRLGCSSVAFTYNDPVIFAEYAIDVAQACRERGIKT
                     VAVTAGYITEDARPEFYRHMDAANVDLKAFTDAFYHRLCFAQLEPVLETLKYLRHETN
                     VWFEVTTLLIPGENDSDAELHRAAEWFAENLGPDVPWHFTAFHPDFKMLDKSHTPSAT
                     LTRAREIARSKGLHYVYTGNVHDSEGGSTWCPGCGSLLIERDWYELGEWNLEKESQTG
                     LSASLGRCRSCGYKIAGRFEEQRGDWGARRLPVRLKSQN"
     gene            complement(5195..6784)
                     /locus_tag="VE486_00025"
     CDS             complement(5195..6784)
                     /locus_tag="VE486_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZA37501.1"
                     /translation="MWGLIQEPCDENVIRSRPATAPCSKSSGRWVLVATTLASSMAFI
                     DGTVVNVALPALQTNLNATIVDVQWVIEAYSLLLSAFLLVGGSLGDHYGRRRVFLIGV
                     ALFAMASTWCGLAPNVHQLIVARAAQGLGAALLVPGSLAIISSSFPEKERGRAIGTWS
                     GFSAITTAVGPVIGGWLIEHLSWRAIFFINVPMALLIILISLWRVPESSDTESKGLDW
                     LGAILGALGLGALVYGLIESSRLGFSNPSVLAALITATALLALFLIIESQIPNPMLPL
                     ALFRSRTFTGANLLTFLLYGALGGTLFFLPLNLIQVQRYSATAAGAAFLPFILIIFLL
                     SRWAGGLVERYGPKIPLFIGPSIAALGFALFMLPGLNAGYWQNFFPPVVVLGTGMAIT
                     VAPLTTTVMNSVAQNRVGIASGVNNAVARGAGLLAIAVLGIVMLQVFKSGLDQRLSNR
                     NLPEAAQSLQAQSTKLAALAVPGNQDPATQQVIRRAIDESFVSGFRIVMAIGAALAIA
                     SAITAMTLITKTPRTDGIERN"
BASE COUNT         1575 a         1935 c         2210 g         1586 t
ORIGIN      
        1 gtaagcgatc cgatgatcaa acatcaaatc cgtatcgccg cggagaagga agagaaagaa
       61 ttttacgaac atcgcgcgcc gaaagcctgg ctggaggcgc tggcgccgat tgggaccgat
      121 tcgcgcaaac cgtttttgga aacagcgccc tggcttatcg ccgtctttgc gcaaccgttt
      181 cgaatcctcg cggacggaac ccgttcgccc acgtattacg cgatcgagtc ggtcggcatc
      241 gcgaccggca tactggtgac ggcagtgcat tcgtgcggct tggccgcctt aacgcacacg
      301 ccgagtccca tgggattcct gaatcgaatt ctcgatcgac cttcgcacga gaaaccattt
      361 gtgctgcttg tggtcggtca tcccgcggaa aatgcgttgg ttcctgatat tacgcgcaaa
      421 ccgctcgacg agatcagctc gtttcggagc aacgcatgag ctaaggaccg caggaaagca
      481 ggaaattctc tccaagttct tgcgttcctg ccttccctgg ctgtttcttc aagaaatgtt
      541 tgtcggacat tatagcgtcg cgtttgcggc gaagagtgag cgaaacagaa ttccgctttg
      601 ggttttgttc atcgctgtgc agttcctcga ttatatctgg gcaacgctcg ttctgctcgg
      661 gattgagaag ctgcgcgtca tcaaaggttt cacggctggg agcatgatcg actcgtatta
      721 ccacccgtat tcgcatagtc tgatcacagc gctcatctgg tcggcggttg ccgctgtgat
      781 ttataaatcc gtgtggcgtg caaaagcttc cagtgctgcc gctttgctcg tgggcgtggc
      841 cgtattctct cattgggtcc tcgacttcat tgcgcatcct cgtgatctgc ccatctacgg
      901 caacacgtgg aaagtcggtc ttggtttttg gaattatcgc ggccccgaat ttgtattgga
      961 ggtggcgctg cttgctggcg gaatcgtgct ttaccaggca cggaacgcga tctcaacagc
     1021 tcgcaagaaa gcggtgatcg gctttgggct cgctttagtc atagtccaaa taggcgacac
     1081 gttcgtgccg cgcacgccgt tgtctgatcg ggccactgct atgggtgtct gggttttcta
     1141 cacccttttc gtcattgtgg cgtacttgct ggagagaggt gcgccggcga gaatcgtgaa
     1201 tcataattca cggtcgcgcc gctgacccag tctgaccatc ccgttcgcat gccactcgct
     1261 aactttcgat ggcgaactct tttgggccaa ctaccgggct agagacacca tcattcattc
     1321 gcccttccga attgaaagga agtcaggcct gggttcatat tcggtgtgat gcagcacagg
     1381 ccagtcggca cggtaacttt tcttttcacc gacatggaag ggtcgagcag ggcgtgggaa
     1441 tcgtatccgt ctgaaacgcg cgcggcgcta cagcggcatg atgagatcat ggcgcgagaa
     1501 gtggaaggtc ataacggtgc gatcattctc gagcgcggcg aaggcgatag cgtattcgca
     1561 gttttcacgc gtgggaacga cgctgtgtct gcggccttcg cgattcagcg tgcatttcaa
     1621 gaagaaccgt ggccaagtca cgttccggtt cgtgtccgca tggcgattca taccggccaa
     1681 gcggccactg attatcgtgg tccggatgtg aatcgtgccg cgcgcatccg agccatcggg
     1741 catggtggcc agattcttgt ttcagatgtc acggcgggaa tcgtgcgcgg aacgctccct
     1801 gaaaaggctg cgctcatcga cctgggtcac catcggttgc gagatctgcc cgggaccgaa
     1861 catgtgttcc aattggcaca tccagagctt cgagaagaat ttccgccgct caaatcgctt
     1921 ggtaattttc gtcagaacct gccagtccaa ctgacgtcgt tcattgggcg ggaaagcgaa
     1981 caaaaaacgg ttcgctccct gataaacgat catcgcgtcg tgacattgat cggctcaggc
     2041 ggttgtggaa agacgcggct tgcaatccag gttgcagcgg acttgctcga gcaatttccg
     2101 gatggtgtgc gttttgtaga cctggcgccg ctcagcgatc cgtctcttgt tctcgatggc
     2161 attgccgcag cggtggaggt caagttggag caaggcgatg ccaaggatga tgcactggtt
     2221 cgaacgctcc agggtacgaa aacgctgatc atcctcgata actgcgaaca tctcgtgcgg
     2281 gcatgcgccg acgctgtgtc agttttgctt cgaactggcg agcacgtgcg catacttgct
     2341 acaagtcgtg aaccgctcgg tctaccgggc gaaagaacat ggcgcgtgcc gtcgctctcg
     2401 ctgcctgatg gcgcgacaag tgtggaagag gttagcgcat gcggagcgat ccagcttttt
     2461 gtcgatagag cagccgcagc acgggaagga tttgcgctga gcgcaggaaa cgcccaaaca
     2521 gtcgtcgata tctgtcgcac gctcgaaggc gtgccgcttg caatcgagct ggccgcggtg
     2581 cgtgtgaagg tgttaagccc acgcgaaatc cgggaccgtt tatcagatcg atttcggctt
     2641 ttgaccggag ggcgcggacg ccatcaaaca ctgcgatcgg cggtcgactg gagctacgat
     2701 ctgctttccg agcaagaacg cggattgttc cggcggctgt cagtttttgc cggcagcttt
     2761 gacctgactg cggtcaaagc gatctggccg gaaagcgatc ctctcgatcg tattgagcag
     2821 ctggtggaca agtcgctggt gacagtggaa caacttaacg acgataagtt gcggtatcgg
     2881 ttgctcgaaa ccttgcggcg gtacggcgcc gagcggcttg tcgaagcggg cgaggaagag
     2941 gatacccgcg agcgacactt tggccattac cttggcgttg ctgagcgtgc ttacgaacag
     3001 cgaatcgaag acgaggcagc ttcactcgcc gccctcgaag ctgaccatga tgatttccgt
     3061 ctagcattgc agtgggcgcg ttcgcgtcca cgggatcttc tccgtctggc ctcggcgctc
     3121 gggtggttct ggcacctgcg atcgtattat cgcgagggaa gatcttggct tgaggaagcg
     3181 ctgagattga acccggccga acgctccggt gaaaaagcgc gcgccttatg ggcgttgagc
     3241 atgattctta actggcaggg cgatgttgcc gcggcgcgcc cgctggcaga agaaagtttg
     3301 gcgttatggc ggaaaagtaa cgatccgctg gaactagctc ttgccttgga aagcatcggc
     3361 tggtcgcact tcgctggtag caattattcc gaagcattgc gttcgatgga agattgtgtc
     3421 gcgtcctacc gcaaacttgg ttcggcgaag ttgatcacgc gcggtcgcgt cgcggtcgga
     3481 caaatgcttg cagcgttggg agatgtcgaa cgcgccgaac aactcgcgcg cgagacgctg
     3541 gccgaaggac gcgcgcaacg ggagccgaag tttgtgcact tctcactcca ctatctggcc
     3601 gactgtgcgt tgtggcgcgg cgatgcgaag aacgcggtgc aactttatgg cgaaagtttg
     3661 cgtgccgctc ttgattatgg aaacgagatg gaagctgcaa ccgaaatgca aggtatggcg
     3721 atgggtttga tgggttctga acgccaagaa gaaggattcc tcctttacgg cgcatcatgc
     3781 gcgcgctttg gagagttgca cacgacaatg atcgatgatg tcgcgttttg ggttaaattt
     3841 cgcgagcgct atcttcctcc agcacgagaa cgcatcggag ctgcggctgc cgatgaagcg
     3901 gaacaggaag gacgtgcgat gggttggcaa aaagcactgg ctcatgcttt cgctgtcgct
     3961 ggcgaaggtg ccattggatt gaatgaggca aaaactgaca caatctgatt atcccggtcg
     4021 ctggtggatg atgcttccgg atgggcgcat cgagtgccgg ctttgtccgc gattttgtaa
     4081 actgaatgac gggcagcgcg ggttttgttt tgtgcggcaa cgcctcggcg atcgaatggt
     4141 gctgactact tatggccgga gcagcgggtt ttgtgtcgat ccgattgaga agaaaccgct
     4201 aaaccatttt ttcccaggaa caagcgtgct ctctttcggc acggcgggct gcaatctcgg
     4261 ttgccgcttt tgccagaact gggacatctc gaaagcgcgc gagtgggaca agctcgccga
     4321 cgccgcgtct ccggagcaga tcgcgctcgc ggcgcagcgg ctcgggtgca gctcggtcgc
     4381 gtttacttac aatgatccgg tgattttcgc tgaatacgcg attgacgtgg cgcaggcttg
     4441 ccgcgaacgc gggatcaaaa cggttgcggt gacagccggt tatatcaccg aggatgcgcg
     4501 ccctgaattt taccggcaca tggatgcggc gaacgtcgat ctgaaagcgt tcaccgacgc
     4561 gttttatcat cggctttgtt ttgcgcagct cgaaccggtg ctcgagacgc ttaaatatct
     4621 tcggcatgaa acgaatgtct ggtttgaagt cactacgctg ctgattccag gcgagaacga
     4681 cagtgacgcc gagctgcatc gcgcggctga gtggttcgcg gaaaatctcg ggccggatgt
     4741 gccgtggcat ttcactgcgt ttcatcccga tttcaaaatg ctcgataaat cgcacacacc
     4801 ctcggcgacg ctgacccgcg cacgcgagat cgcccgctcg aaagggctgc attacgttta
     4861 cacgggaaac gttcacgaca gcgaaggcgg aagcacgtgg tgtcccggtt gcggctcgct
     4921 gctgatcgag cgcgattggt acgagctcgg cgaatggaat ctcgaaaagg agtcgcagac
     4981 aggattgtct gcgtccctcg gcagatgtcg atcttgcggt tacaaaatcg cggggcgttt
     5041 cgaagagcag cgcggcgatt ggggcgcgcg ccgtcttccg gtcaggttga aaagtcaaaa
     5101 ttagagaaga tgcaactgac gcgttgggga aatttctctg cagagcgaac cgtctagaca
     5161 gcgagatggc gggggagggg acacgcgtca acagctagtt tctttcgatg ccgtcggtgc
     5221 gcggtgtttt tgtgataagc gtcatcgccg tgatcgcgct agcaatcgcc agggccgcgc
     5281 cgatggccat cacgattctg aagccggaaa caaaagattc atcaatcgcg cggcgaatca
     5341 cctgttgagt tgccgggtcc tgattccccg gaacggcgag ggctgcgagt ttggtggatt
     5401 gagcttgcaa cgattgcgct gcttcgggca ggttccggtt ggatagtcgt tgatcgagcc
     5461 cgctcttaaa gacctgaagc atcacgatcc caagaacggc gatggcgaga aggccggctc
     5521 ctctggcgac tgcgttgttg actcccgatg cgatgccgac ccgattctga gcgaccgaat
     5581 tcatcacggt cgtggtaaga ggagcaacgg ttatggccat gcctgttcca agcacaacaa
     5641 caggagggaa aaagttctgc cagtaaccgg cgttcaggcc gggcaacata aaaagcgcga
     5701 agccaagggc tgcaatcgag gggccgataa acaacgggat tttcggtcca tacctttcga
     5761 cgagcccgcc cgcccagcgc gaaaggagaa agatgattaa gatgaacggc aggaaagctg
     5821 cgccagccgc ggtggccgag taacgctgga cttgaatcag attgagcggc agaaaaaaca
     5881 gagtgccgcc gagcgctccg tacaacagga acgtaagcag gttcgcgccc gtaaacgttc
     5941 gcgatcggaa cagagcaagc ggcagcatgg gattaggaat ctgcgactcg atgatcagga
     6001 agagagcgag cagggcggta gcggtaatga gcgctgcaag cactgatgga ttactgaaac
     6061 ccagtcgcga cgattcgatt agtccataga ccagcgcgcc caggccgagc gctcccaaaa
     6121 ttgcgcccaa ccagtcgagt cctttgctct ccgtgtccga gctctccgga acacgccaca
     6181 gtgagattag gattattaga agcgccattg gaacattgat gaagaatatg gcgcgccagg
     6241 aaagatgttc gattagccag ccgccgatca ctggcccgac agcggtcgta atcgcactga
     6301 aacccgacca ggtaccaatc gcgcggccac gttctttttc gggaaacgaa ctgctaatga
     6361 tcgcgagact gccgggaacg agcagggctg cgccgagtcc ttgtgctgcg cgcgctacaa
     6421 tcagttgatg tacgttggga gcgagtccgc accacgtgga cgccatagca aagagagcga
     6481 caccaatcaa gaaaacgcgg cgccgtccgt agtgatctcc gagcgaaccg ccgacgagca
     6541 gaaaagccga aagcaaaagc gagtaagcct cgatgaccca ctgcacgtca acgattgtgg
     6601 cattcagatt cgtttgcagg gctggcaacg ccacattcac aactgtgccg tcgataaagg
     6661 ccatactcga agcgagggtt gtagccacaa gcacccagcg accgctcgat tttgaacacg
     6721 gcgctgttgc cggtcgcgac cggattacgt tttcgtcaca aggttcctga atgagtcccc
     6781 acatatttgt gtcaaatccc cggacgactg gtcctatcaa ggagagatcg tttacatcgg
     6841 tagcgcatta tttcctgtag ccaccggcct atggccggtc aaaacgagcg cgccaaatca
     6901 tcgttaggat tgaacggccc acagggccgt ggctacaacg tgacctatcg ttgccaacct
     6961 tccctcgcac cggcatgggc tgttaatatt cgcaaaacgc gtcgtcggca tagcagtaga
     7021 gccagcgctc gctgggctgg gcggaagcga tgaccggatg cccgctggcg tgcgcgtgtt
     7081 agctggcatg gcgattcagt gagctatcgc agcacaatgt aacgccacat tgctgacacg
     7141 tccgcagatg gacccatgct gcgccgatct tcacacgctc agcgcactcg cgaatttgcg
     7201 gctgtttgac ggatgcgatg gcgtcgagat gagagcagcc tcggtcagac attgttatat
     7261 tctcgcagca ctgtcggttt gcacatgtcc tatttaggac ggctga
//
LOCUS       DASQUQ010000002         4852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_901473, whole genome shotgun sequence.
ACCESSION   DASQUQ010000002 DASQUQ010000000
VERSION     DASQUQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901473"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VE486_00030"
     CDS             <1..277
                     /locus_tag="VE486_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37502.1"
                     /translation="ADAERARAIVDLVNDRATLLCLRAEREFLRLLHGDCNSPVGVLA
                     NIDNGKLRLRAQVFDQKSTGPREGKVEGGLEDGEVLAAELLRRINVA"
     gene            299..1822
                     /gene="cobA"
                     /locus_tag="VE486_00035"
     CDS             299..1822
                     /gene="cobA"
                     /locus_tag="VE486_00035"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZA37503.1"
                     /translation="MSTSTKRKGKVYLVGAGPGDLGLVTLRAKECIESADVIVYDHLA
                     NPEMLSWARDDAEIIYAGKAPGEPSPGQLEINTLLVEKAHEGKNVVRLKGGDPFVFGR
                     GAEEAKAIADAGIEFEIVPGITSAIAGPAYAGIPMTHRAENSHVTFFTGHEDPAKTES
                     AIDYAALAKLGGTQVMLMGVERLGAITREMLNQGVRQDLPVALVRWATTGRQETLTGT
                     LQDIAQRAVASGFAAPAVAVFGEVVALRESLKWYENRPLSGKRIVVTRTRKQASVLSN
                     KLRALGAQVIELPTIRIEPPSDLRAFAELVQDAHVYDWIVFTSANGVDAFFEIFFKLY
                     DDAREIGGARIAAIGPATAQRVKDFHLHVDLQPDEFVAEAVVKEFQKVGSVENLRILL
                     VRAEKTRDVLPNELSGLGAIVDEAFAYRTVPEARDTTGARRRLAKEAADLITFTSSST
                     VENFLALGLPWPKGMRVASIGPITSKTARDHGLTVDIEARRHDIEGLVQAICDFFAP"
     gene            complement(1926..2123)
                     /gene="rpsU"
                     /locus_tag="VE486_00040"
     CDS             complement(1926..2123)
                     /gene="rpsU"
                     /locus_tag="VE486_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZA37504.1"
                     /translation="MPEVIVRKGEPVDRALKRLKNKLDAEGILEEVRRLRAFETPSQK
                     HRRKAKANAKRGRTKFRFNPS"
     gene            complement(2315..4696)
                     /gene="secD"
                     /locus_tag="VE486_00045"
     CDS             complement(2315..4696)
                     /gene="secD"
                     /locus_tag="VE486_00045"
                     /inference="COORDINATES: protein motif:HMM:NF009583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZA37505.1"
                     /translation="MTPALTFFIGLLMLVLFGWYFATDQGLRKRLLAATLMLLLVAFS
                     IVTIWPPQKKIHLGLDIQGGTSFLIRLKPGDKPITKAMLDQAVEVIRKRVDYFGGGEP
                     IISPVGQDRILVQIPGLDVAKIQEARDQLSRVAKLEFRLVYRDNGERLKAIDEGKEVI
                     PPDYRIEVYKIGAEEAPMTNEFGETVAGAKEKSREERLLVKKKADLTGDRVSASNAYY
                     GNEGWTVQLKFDGEGAKKFGEITEQYKGYRFAIVLDGAIQSAPVIRDAIYGGDAVITG
                     SFTEEEARSLASVLENPLQTPVSIEEERSVSPTLGMDSIRASIFAALLGLSITLLFAL
                     IYYKLAGLIADLAVIINLILLLGALTMFGFVLTLPGIAGIILTIGMAVDASVLIYERL
                     REEMAAGKTLKAALPAAYNKAFSSIFDANVTTLITAGILFWKASGPVKGFAITLTLGI
                     LASLFTALIVGRNALGWFVDTGRIKRISMLHLISSQHINFLGKGFLACMCSLALIIAG
                     ATAFYIRGERNFGVDFRGGDLVTLSAPGKLDTAQVRGALKPIGFADASIQESRQGDRS
                     YITIRTPLNTSDKVEQQLEQALPNAGLRIEGSERVGALVGGELARSSLIALGLGILGI
                     LIYITFRFELSFAVGGIFALLHDVIITVLMFALLGRELTLAMVGAVLTIAGYSINDTI
                     VVYDRIREGLVSGRRGTIEQIMNESINQTLSRTILTSSTTLIPLCFLFFFGGPVLRDF
                     SLAIIIGIAVGTYSSIFIAAPIVLWWTRARGGATVLRREITQQAATANPLAQR"
BASE COUNT         1104 a         1312 c         1411 g         1025 t
ORIGIN      
        1 ggcagacgcc gaaagagcgc gagcaattgt cgatcttgtg aacgatcgcg cgacgttact
       61 ctgcttgcgc gcggagcgag aatttctgcg tttgttgcat ggtgactgca attcgcccgt
      121 gggagtgctg gcaaacatcg acaacggcaa gctgagattg cgtgcgcaag ttttcgatca
      181 aaagtcgacc gggccacgtg agggcaaagt tgaaggcgga ctcgaagatg gcgaggttct
      241 cgctgcggaa ctactaaggc gaatcaatgt ggcgtgagaa tcgatcacga ttacgagcat
      301 gagcacgagc acgaaacgga aaggcaaagt ttatcttgtt ggtgcggggc cgggcgacct
      361 tggtctggtt acgttgcgag caaaggagtg catcgagagc gcagatgtga tcgtttacga
      421 ccatttggca aacccggaga tgctcagttg ggcgcgcgat gatgcggaga ttatttatgc
      481 cggcaaggcg ccgggcgaac cctcgccagg ccagctggaa attaacacgc tgctggtcga
      541 gaaagcgcac gaaggaaaga acgttgtccg gttgaagggc ggtgacccct ttgtctttgg
      601 tcgaggcgcg gaggaagcaa aggcaatcgc tgacgcggga atcgaatttg aaattgtgcc
      661 gggaatcacc tcggctatcg ccggtccagc ctacgcagga attcccatga cgcatcgcgc
      721 ggaaaattcg cacgttacat tttttaccgg ccacgaagat ccggcgaaga ctgaaagcgc
      781 catcgactac gcggcgctgg caaaacttgg tggtacacaa gtgatgttga tgggcgtgga
      841 acgtcttgga gcaatcacgc gcgaaatgct taaccagggt gtacgccagg atttgccagt
      901 ggcgctggtg cgctgggcga cgaccgggcg ccaggaaacg ctcaccggga cccttcagga
      961 tattgcccaa cgagctgttg ccagtggttt tgccgcgccc gcggtcgcgg tattcggaga
     1021 agttgtcgcc ctgcgtgaaa gcctcaagtg gtacgaaaac cggccgctct cggggaaacg
     1081 gatcgtagtt acacgcaccc gaaaacaggc gagcgtttta agtaacaaac tgcgcgcact
     1141 cggcgctcag gtcatcgaac ttcccacgat tcggatcgag ccacccagcg atttgcgcgc
     1201 gttcgcagaa cttgtgcaag acgcgcatgt ttacgactgg atcgtcttca ccagcgccaa
     1261 tggcgtggac gcgtttttcg agatcttttt caaactctac gatgacgccc gcgaaatcgg
     1321 cggcgcgcgg atcgcagcca tcggcccggc tacagcgcag cgggtgaaag actttcatct
     1381 tcatgtcgat cttcagccgg acgaattcgt ggcggaagcg gtggtaaagg agttccaaaa
     1441 agtaggcagc gtcgaaaatt tgcggatcct cctggtgcgc gcagaaaaga cgcgggatgt
     1501 gcttccaaat gaactctcgg gcctgggggc aattgtggac gaagcgtttg cttatcggac
     1561 ggttccggaa gcgcgcgaca ccaccggcgc gcgccggcga ctggcgaagg aagcggccga
     1621 tctgatcaca tttacgagtt cctcaacggt ggaaaatttt ctcgcgcttg ggctgccctg
     1681 gccgaaagga atgcgcgtcg ccagcatagg ccccatcaca tcaaagactg cgcgcgatca
     1741 cggtttaaca gtcgatatcg aagcgcggcg gcacgacatc gagggactag ttcaagcaat
     1801 ttgcgacttt tttgctccct gaactgtgag caaaaagggt taggacgcgg ccaatttcgc
     1861 aatccgcaac tccgacatca ggtcgcaagc acgcgaagaa tttcggtgtt ggcaatctga
     1921 agttatcagg atgggttgaa tcggaacttg gttcgaccgc gcttggcgtt ggcttttgct
     1981 ttgcggcgat gcttttgtga aggcgtctcg aaggcgcgca aacggcgaac ctcctcgaga
     2041 ataccctcag catccagctt gttcttcagc cgctttaacg cgcgatcgac gggttcacct
     2101 ttgcgaacga tcacttctgg catcgataat cactcctggg cttaaaagat gggttgctta
     2161 accggcgagg cggagcctac tcgggccggg cgaccgcgtc aaatctggcg aaaaatccgc
     2221 ccgagcgggc tgtttttccc ggaatgggtc gcatgtgccc catagatccc atttttccat
     2281 gggactttgc aaggaacggc ttagcgatcc gccactaccg ttgggcaaga ggatttgctg
     2341 tggccgcttg ttgcgtgatt tcgcgccgca ggacggtcgc gccgccgcgg gcgcgcgtcc
     2401 accaaagaac gatgggcgca gcgataaaga tcgaggaata agtcccgacc gcaataccga
     2461 tgatgatggc gagagagaaa tcccgcagca ccggcccacc gaagaaaaac aagaagcaga
     2521 gcggaatcaa ggtcgtgctg ctggtcaaaa ttgtgcggct caatgtctgg ttaatgcttt
     2581 cgttcatgat ctgctcgatc gtgccacgcc ggccactgac caatccctct cgaatccggt
     2641 cgtaaacgac gattgtgtcg ttgatggaat aaccggcgat ggtgagcact gcgccaacca
     2701 ttgccagagt cagctcccgt cccagcaaag cgaacatcag gacggtaata attacatcgt
     2761 gcaaaagtgc gaagattcct cctaccgcga aggagagctc gaagcgaaac gtgatgtaga
     2821 tcaggatgcc gagaatcccc agaccgagcg cgatcaacga acttctggcc aattcgccac
     2881 ccaccagcgc acccacccgc tccgaacctt cgattctgag gccggcattt ggcagcgcct
     2941 gctcaagctg ttgctcgacc ttgtcgctgg tgttgagcgg ggtgcggata gtgatgtaac
     3001 ttctgtcgcc ttggcgtgac tcctggatcg aagcgtcagc gaagccgatc ggcttaagcg
     3061 cgccacgcac ttgtgcggta tcgagtttcc cgggcgcgct caaggtgaca aggtccccgc
     3121 cgcggaaatc gactccgaaa tttctctccc cccgaatgta gaacgcagtg gcgcccgcga
     3181 tgatgagcgc gagcgagcac atgcaggcca gaaaaccttt gccgagaaag ttaatgtgct
     3241 gcgatgagat caaatgcagc atcgagatgc gtttgatccg gcccgtgtcc acaaaccagc
     3301 ccaaggcatt tcggcccacg atcagtgcag tgaagagcga tgccagaatg cccagcgtga
     3361 gcgtgatcgc aaagcccttg actggtccgc ttgctttcca aaacaagatc cccgccgtaa
     3421 tcagcgtagt gacgttggcg tcaaaaatcg agctgaaagc cttattgtaa gcggcgggca
     3481 aggcagcttt gagtgttttg ccagcggcca tttcctcgcg caaccgttcg taaatcagca
     3541 cgctcgcgtc cacagccatg ccgatcgtta agatgatacc ggcgatgccg ggcagggtga
     3601 gcacaaaccc gaacatggta agcgccccga gcaacagaat gaggttgata atgaccgcca
     3661 gatccgcaat gagcccggcc agcttgtaat agattaatgc gaaaaggagc gtgatactga
     3721 gacccagcaa agccgcaaaa atgctcgcgc gaattgaatc cattcccagc gtgggcgaga
     3781 cgctgcgttc ttcctcgatg ctgaccggcg tttgcaacgg gttttcaagt acgctggcca
     3841 gagagcgcgc ctcctcctcg gtaaaacttc cagtgatgac ggcgtcgccc ccgtagatgg
     3901 catcgcgaat cactggagcg gattgaattg cgccatcgag aacgattgcg aagcgataac
     3961 ctttgtattg ctcggtgatc tccccaaatt tctttgcccc ttcgccgtcg aacttcaact
     4021 gaacagtcca accctcgtta ccgtagtagg cgttcgatgc cgaaacccgg tcgcccgtga
     4081 ggtcggcttt cttttttacg agcaaccgtt cttccctgga tttttctttg gcgccggcga
     4141 cggtttcacc gaattcgttg gtcatcggtg cctcctccgc gccgattttg taaacttcga
     4201 tccggtaatc tggcggaatc acttccttgc cttcgtcgat cgctttcaag cgttcgccgt
     4261 tgtcacgata aaccagacga aactcgagtt tggctacgcg cgagagttga tcgcgcgctt
     4321 cttgaatctt ggccacgtca agccccggaa tttgcacgag gatgcgatcc tgaccgaccg
     4381 ggctgatgat tggttcgccg ccgccgaaat aatcgacgcg cttgcgaatg acttcgaccg
     4441 cctggtcgag cattgccttg gtaattggtt tgtcgccggg cttgagtcga atgagaaacg
     4501 aagtgccacc ttggatatcg aggccaaggt ggatcttttt ctggggcggc cagatggtga
     4561 cgatgctgaa ggcaaccagg aggagcatca gcgtagctgc gagtaggcgt ttccgcagac
     4621 cctgatcggt cgcaaagtac cacccgaaaa ggacaagcat gagcagccca ataaaaaatg
     4681 tgagcgcggg agtcatctct ttgtcattcc aagcgaagtc gaggaatctc tcactgaatc
     4741 accgttccaa ttgcggattt ttccatctcg atctttacgt tgtcggctac ttttacgatc
     4801 accgtcgtgt ccttcacgtt agagataaga ccgtgaatgc cggcattggt ta
//
LOCUS       DASQUQ010000003         5121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_901881, whole genome shotgun sequence.
ACCESSION   DASQUQ010000003 DASQUQ010000000
VERSION     DASQUQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901881"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1315
                     /gene="mnmA"
                     /locus_tag="VE486_00050"
     CDS             176..1315
                     /gene="mnmA"
                     /locus_tag="VE486_00050"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZA37506.1"
                     /translation="MNQNKGRVLLGMSGGVDSSVAGYLLREQGYDVIGVTMKVWPQDC
                     ISRAEDKCCGPQAVADARSVAHSLGFPHYVVDEADQFERLVIDYFSSEYQAGRTPNPC
                     VMCNEKLKFGNLWSKAAALECDYIATGHYAIIEHSVGGGGDGDAGYAILRKGADLRKD
                     QSYFLFSLRQPQLRRALTPLGRMSKPQIREIARSLGLKVADKIDSQEICFVPGNDYKA
                     FLRSHLGEAEFHRGEIYDVDGNFIAEHDGIELFTIGQRKGLPGGSPRPRYVVDLDPET
                     NRVIVGDADDLVCDEFEVDRVNWHAVAKIDDPSYNEEIEATVKIRYSHPGTRATLIRL
                     DNGRAHIRLHEPQRAVTPGQAAVFYGGDVVLGGGWICRQEATILA"
     gene            1319..1642
                     /gene="cutA"
                     /locus_tag="VE486_00055"
     CDS             1319..1642
                     /gene="cutA"
                     /locus_tag="VE486_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZA37507.1"
                     /translation="MPAKILLALSTFPDAETARRISNQLVTETFAACANIVPAVESIY
                     RWKEKIETAGEVIVFFKTTPDRQRGFQEKLRSLHPYDVPEIIFVPVTHGLPEYLRWVA
                     ENCGG"
     gene            complement(1813..3246)
                     /locus_tag="VE486_00060"
     CDS             complement(1813..3246)
                     /locus_tag="VE486_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZA37508.1"
                     /translation="MISELNRAERAVAEHSVELKKPLGLRDLVLTQIVFVVGSSWVGT
                     AAKLGPAHLFFWLLAILLFYVPQAAVVIYLNRLMPLEGGIYQWAKFAFNEFAGFIVAW
                     NLWLLSIMVIALGGMFITTNLSYAFGDAGAWMRNSPWCVSLISCALVGSLGWTSVRGL
                     SLGKWLHNIGGFAMLLAYAALIVLPFVMLARGNLSAYHPLQIAAPTMSIFYCVNIFTK
                     LAVGALSGFEYVDILAGETRAPARNIGRSVMIAAPIIALMFILGTSSVLAFIGDNPID
                     LIGPVPQTLRLGLHSFRIAGAIASIAILLMTVRSISSISIHFTGSSRLPMVAGWDHLL
                     PPWFSRLHARYKTPINSIIFVGAVTLVIGISSQIGAGMQEAFQLVDNAANVFYGIVYA
                     VMFAIPIVGAAAVRARARIWLRLAAACGLAVSLLAIFFTIYPIIDVPSPLIFAAKIVL
                     VTVIANAIGIAIFALGEKRRRAAQSRE"
     gene            complement(3428..3952)
                     /locus_tag="VE486_00065"
     CDS             complement(3428..3952)
                     /locus_tag="VE486_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37509.1"
                     /translation="MKIGFFLSQSLLRDLRQPEYIHVLINPLPIYGLVMGWIGLIIAL
                     FLKSRRAQIATLSLVLISCVSAWPAFEFGEQGYDRVLSMTDEAGHAWLDEHMHRAEQF
                     IYVFYALALLTVAAIAVPIKWPKSAGPLVISVLLLGAAAFGAATHIAYAGGRIRHREF
                     RNESPPSIRAEHEH"
     gene            complement(4230..4928)
                     /locus_tag="VE486_00070"
     CDS             complement(4230..4928)
                     /locus_tag="VE486_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZA37510.1"
                     /translation="MSRRVREFGFYSMRRYTCLAIMLLVAAGSAAQSDGAEASKLELL
                     DVRSIDPSIAVELRYAGPNNLTGHPLYPPGTRALVRPEVAQRLAVAQRFLRQHGYGLK
                     IWDAYRPRSVQAQLWRAAPATDYVADPETANGSLHMWGVAVDATLVNSRNQPVMMPTD
                     FDDLTPAAMLIYRGANRYIRSNLTLLQAAMGAAGFYGQRREWWHFIAPDWKKHVPDPD
                     LKVSVSGKSSKSKS"
BASE COUNT         1192 a         1386 c         1500 g         1043 t
ORIGIN      
        1 atgttggttt cggcgtcgcg ccgaaacaat ctttccttaa aatgagattt tgagcgattg
       61 cggcgcgtaa gagaagttcg cgatgtcgag gacgccatcg ccagcacgcg agacgcgtac
      121 gctaccccgg acgccgtttg acgcgcgatg ctctgcattc accttagtcg ctgcaatgaa
      181 ccagaacaaa ggacgcgttt tgctcggaat gagcggtgga gtggactcca gcgtggcggg
      241 gtacctgctg cgcgagcagg gctacgacgt catcggtgtg acgatgaaag tctggccgca
      301 ggattgcatc tcgcgcgcgg aagacaaatg ttgcggcccg caggcagtcg ccgatgcgcg
      361 ctcggttgct cattcgttgg gatttccaca ttacgttgtc gacgaggccg atcagttcga
      421 gcgcctggtc atcgattatt tctcgagcga gtaccaggcc gggcgtacgc cgaatccatg
      481 cgtgatgtgc aacgaaaagc tgaagttcgg gaatctctgg agcaaagcgg cagcactgga
      541 gtgcgattac atcgcgaccg gccattatgc gatcatcgag cattctgtag gcgggggcgg
      601 ggacggtgac gccggttacg ccattctgcg aaaaggcgcc gatctgcgca aagatcagtc
      661 ttattttttg ttcagcctgc gacaaccaca actgcggcgg gcgctcacgc cgctcggcag
      721 aatgtccaaa ccgcaaattc gcgaaatcgc gcgctcgttg ggtctaaagg ttgcggacaa
      781 gatcgacagc caggaaatct gctttgtgcc cggcaacgat tacaaggcat ttctgcgttc
      841 ccatcttggc gaagcggagt ttcatcgtgg cgagatttac gatgtcgatg gaaatttcat
      901 cgccgaacac gacgggatcg agcttttcac aataggccag cgcaaagggt tgccgggcgg
      961 ttcgccgcgg ccacgttacg ttgtcgatct ggatccggag acaaatcgcg taatcgttgg
     1021 cgacgctgac gaccttgtct gtgacgaatt tgaagtcgat cgcgtgaact ggcacgctgt
     1081 ggcaaagatc gatgacccga gctacaacga agaaatcgaa gcgacggtca agattcgcta
     1141 cagtcatccg ggcacgcgtg ccacccttat ccggctagac aatgggcggg cacacattcg
     1201 cttgcatgaa ccgcaaagag cagtgacacc cggtcaagca gctgttttct atggcggcga
     1261 tgttgtgctg ggcggtggat ggatttgccg gcaagaagcg accattctcg cgtgacgcat
     1321 gccagcaaag attctcctgg cgctcagcac atttcctgat gctgaaaccg cgcgtcgaat
     1381 ttccaatcaa ctcgtcacgg aaacatttgc cgcgtgcgca aatatcgtgc ccgctgtcga
     1441 atcgatctac cgctggaaag aaaaaattga gaccgccggc gaagtaatcg tctttttcaa
     1501 gaccactccg gatcgacaaa gaggttttca ggaaaagttg cgatcacttc atccgtacga
     1561 tgtgcccgaa ataatttttg taccggtcac gcacggattg cctgagtatt tacgatgggt
     1621 cgcggagaac tgcggcggat agccggagct tctggggagc acgggccact ggcctgtagt
     1681 tcgcggcagc ttgccgcgaa cgctcctggt acactttgca cgttggcgtg accagatgct
     1741 ttcgggcagc ggccggatgc tacaggccgg cggcctgtgc tcgccagaag cagaacgcac
     1801 gcaaacgtga tatcactctc gcgattgggc cgcacgcctg cgtttttcgc ctagcgcgaa
     1861 aatcgctatg ccaatcgcgt tcgcaatcac tgttaccaaa acgatttttg cggcgaaaat
     1921 tagcggactc ggcacgtcaa taatcggata gatggtgaag aagatcgcga gtagagaaac
     1981 ggccaaaccg caagcagcag cgagtcgcag ccagatgcgc gccctggcgc gtaccgctgc
     2041 cgcgcctacg attgggatgg cgaacatcac ggcatagacg attccgtaaa aaacgttggc
     2101 ggcgttgtcc acgagttgaa atgcttcctg cattcccgcg ccgatttgac ttgagatacc
     2161 aattacgagc gtgaccgcac cgacaaaaat gattgaattg atgggcgttt tgtagcgtgc
     2221 gtgcaggcgg gaaaaccacg gcggcaacag gtggtcccag ccggccacca ttggcaggcg
     2281 cgagctcccg gtgaaatgga tgctgatgga agaaatcgag cgcactgtca tcagcagaat
     2341 cgcgatggag gcgatcgcgc cggcgattcg gaatgaatgc aggccgagtc gcaatgtctg
     2401 cggcaccggt ccgatcaggt cgattggatt atctcctatg aaagcgagga ccgaactcgt
     2461 tcccagaatg aacatcagcg cgatgatcgg cgcggcgatc atgacggagc gcccgatgtt
     2521 gcgcgccggt gcgcgagttt caccggccag aatgtcaacg tattcgaagc cgctgagcgc
     2581 gccgaccgcc agttttgtga agatattgac gcaatagaag atcgacatcg tgggtgcggc
     2641 gatttgcaac gggtgatatg cgctgagatt gccccgcgcg agcataacga aaggcaaaac
     2701 aatcagggcg gcgtaagcca gaagcatggc gaacccgccg atgttgtgga gccatttgcc
     2761 caatgataat ccgcgaacac tggtccagcc aaggctgccg accagcgcgc aactgatgag
     2821 tgagacgcac cacgggctgt ttcgcatcca cgcgcctgcg tccccgaagg cataggagag
     2881 gtttgtggtg atgaacattc cgcccaaagc gatcaccatg atcgacaaga gccagagatt
     2941 ccatgccacg ataaatccgg cgaattcgtt gaacgcgaac tttgcccact ggtaaatccc
     3001 gccttccagc ggcatgagcc ggtttaaata aatgacgacg gcggcttgcg ggacgtagaa
     3061 cagtaaaatg gcaagtagcc agaagaagag atgcgcaggt ccaagcttgg cggcggtgcc
     3121 gacccagctt gagccaacga caaagacaat ctgcgtgagg acgagatcac gcaggcccag
     3181 cggctttttg agttcaacgc tgtgctctgc aacggcgcgc tccgcgcgat tcagctcgga
     3241 tatcatcgtt gaaagggtcg actcgttaaa gcgttaaagc ggcaccgcgt atcgtgtcat
     3301 gttgagcgaa gcgaaacatc tctgatttct caggttgacg ttgccggaag aactagtcag
     3361 agattcttcg ctccgctcag aataacaatg gagtttcggt tttagcgctt caacggcttg
     3421 gggatgctca atgctcgtgt tcggctctga tcgagggcgg cgactcgttc cgaaattcgc
     3481 gatgacgaat tcgcccgccg gcgtaagcga tgtgggttgc cgcgccaaac gctgccgcgc
     3541 cgaggagaag cacggagata acaagcggcc cggccgattt tggccatttg atcggaactg
     3601 caatggcagc aacggtcaaa agagcgagcg cgtaaaaaac atagatgaac tgttcggccc
     3661 gatgcatgtg ctcatcgagc caggcatgcc cggcctcatc cgtcatactc agcacgcggt
     3721 cgtaaccttg ctcaccaaac tcgaaggctg gccaggccga aacgcagcta atcagcacaa
     3781 gcgacaatgt cgcgatttgc gcacgccgac tctttaagaa gagcgcgatg atgagtccga
     3841 tccaacccat caccaacccg taaatcggca gtggattaat caggacatga atgtattccg
     3901 gttgccgcaa atcccgaagc aggctttggc tcaaaaaaaa tccgattttc atcgcggaac
     3961 tcttctcacg aaataagagt cggcaaagga tagatttttt tgagccaaac acggcttcgc
     4021 cgtggcgaca gcgagcgcct gcgtggtagc ggcagcgcat cttgcctgcc tgcgcgcagc
     4081 gcgattaata gcctctgtga ggtcgctcgg cgcctgcaac acgctttcgg tcgttcctcc
     4141 ctcggtctcg gcgcgcaaga tgcgacgcgc taccctccct tcggtcgcgg attaatcccc
     4201 tcacagagga tattaatcca aggtatcgat cacgatttgg atttggacga tttcccggac
     4261 acgctgactt tcagatctgg atccggcaca tgtttcttcc agtctggcgc aatgaaatgc
     4321 caccactccc ggcgctgtcc gtaaaagcca gccgctccca ttgccgcttg taacagggtc
     4381 aaattcgatc ggatataccg attggcgccg cgataaatta acatggcagc cggagtgaga
     4441 tcgtcgaaat cagtaggcat catgacgggt tggttgcgag aattgaccag agttgcatca
     4501 accgccacgc cccacatgtg gagcgaacca ttggcagttt ccggatcggc gacgtaatca
     4561 gttgcaggcg cggcgcgcca gagttgtgcc tgcaccgatc tggggcgata cgcgtcccag
     4621 attttcaggc catacccatg ctggcgcagg aatcgttgtg caactgcgag acgctgtgcg
     4681 acttcgggcc ggaccagggc ccgggttccg ggaggataca atggatgtcc ggtaagatta
     4741 tttggaccgg cgtatcgcaa ctcgacggca attgaaggat caatggatcg gacatccagg
     4801 agctcaagct tgctcgcttc agctccgtcc gattgtgcag cggagcccgc ggccacaagc
     4861 agcattatcg ccaggcatgt gtaacggcgc atcgaataaa aaccgaattc tcggacacgc
     4921 ctggacatgt cgattctatg tttcaaacgg tggacagcgc gtgcgcgatc actgcgctgt
     4981 gataactggg ccggcatcgg tctttgcgag acgcgcgagc agcacgcggg cgctatcgtc
     5041 cgggatggtg aaacgcaatt ccaaattcac gtcgccctgc ctttgaattt cgggcggttg
     5101 agagtagagc aacaattccg a
//
LOCUS       DASQUQ010000004         5005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_541549, whole genome shotgun sequence.
ACCESSION   DASQUQ010000004 DASQUQ010000000
VERSION     DASQUQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541549"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VE486_00075"
     CDS             <1..583
                     /locus_tag="VE486_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA37511.1"
                     /translation="QWSANVFYNLGNAYFRAGDFGRAILNYERALALERHHPEATANL
                     QIARDEARALEMQPSWPERYLQFASINQYSVAGAIAFWIALFCVVGLIFGRRRSAAII
                     ALSIFSLLVFVVAVLAIYELDHGSKGRALAIVTGKDVQARVATADTANSVLVLPPGSE
                     VKILNARGDWVYAALPNNLRGWIPAKSAEQVRL"
     gene            771..1559
                     /locus_tag="VE486_00080"
     CDS             771..1559
                     /locus_tag="VE486_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZA37512.1"
                     /translation="MKVAVAQISCSLGNPDANLIKTRDFSARARDAGAELIVFPEMID
                     TGYSMPVIQTHAAPWTSGFVPGLQEIARELSIAIVSGVSERDGALIYNSQIFVDQQGN
                     IIAKYRKTHLYAVAPVEEQKCFAHGDAFTDFAIGGLRFGFSICYDLRFPEVYRKLVAD
                     QNVGAFIVSSAWPFPRLEHFRILSVARAIENQSYVIASNRVGKDDDLWFCGSSAIIDP
                     RGIVIAAASADREELIQADLSEELVLSVRRRVESFAHRRKNLYK"
     gene            complement(1617..3662)
                     /locus_tag="VE486_00085"
     CDS             complement(1617..3662)
                     /locus_tag="VE486_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HZA37513.1"
                     /translation="MSLWKKLLWFAVSALGVGAIAILGISRGEQISALWIIIAGLCAL
                     SISYRFYSKWLAAKVLMLNDERATPAVVQNDSKDYVPTNRWMVFGHHFAAIAGPGPLV
                     GPVLAAQFGFLPGTLWILIGATLGGGVHDMIMLFASIRRGGKTLGQMVKEEIGRGVGA
                     LALISVLAIMIILLAVLALVVVQALAESPWGVFTIAMTIPVALIMGIGLRTGKVSVMA
                     VTIFGLLGLAFGVWGGQFLADFPAIEAWFRHDQKWLAWAIMIYGLAASILPVWMLLTP
                     RDYLSTFLKLGTVVMLAAAVVLINPTLQMPAITKFIDGSGLVFAGPVFPFVCITIACG
                     AVSGFHSLIASGTTPKMVRRESRIRTIGYGAMVTEMMVALMAMIAACVLQPGEYFAIN
                     TKGTPAEVVARVSASGFPVTETEMQTLATNLGESTMFNRAGGAPTFAVGMAHMFARVS
                     ASPTALGLWYHFAIMFEALFILTTIDAGTRVGRFLLQDFLGTIWRPLGNTRSWSANVF
                     SSVLLVAAWGWFLYVGVIDPLGGINSLWPLFGLANQLLSVVALCLGTTILIKMHKAKY
                     LFVTVLPLVFMCVVTFSAGYLKVFSPDPRLGFLSGARSLMHEAAGIADPAKAADLARQ
                     AGVWRFDAVVALFFLVLVLLIVIGSARQWWQLLRGTRRVVLHESEFVPISAAQLAEF"
     gene            3712..4452
                     /locus_tag="VE486_00090"
     CDS             3712..4452
                     /locus_tag="VE486_00090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZA37514.1"
                     /translation="MQLSHKIKKLLGGQGIASARKHTLRSLRANRFPLEKKRVIETID
                     PAAFEQIRRRYAVNKPTDDWRKYLDLDRWIAINIRRVRDIELDLARPKRILDLGCGAG
                     YFLYIAQLLGHSGLGLDLDRQPMFAEITRLLGVHRVIQRVQAFHPLPALGEKFDLITA
                     FMICFNNHRRPSLWEVAEWNFFLDDIAKHLAPRGRVWLELNRETDGTLYTPELKEFFQ
                     KRGARIDEHKVMFNSGLLAPASTSPVAR"
     gene            complement(4403..>5005)
                     /locus_tag="VE486_00095"
     CDS             complement(4403..>5005)
                     /locus_tag="VE486_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZA37515.1"
                     /translation="LSAVLRLLHPFMPHITEELWSLLGFGKDTIQFAPLPERMPLDKV
                     DVAGKRQFVLDIYKTVQAGRNLRAEAKLPSNRKIHFILRTDEKAISDEIPTISRLLNA
                     AELKLDQQYEAQPGSPVAVTPLGEILLATAAADKAAESGRLDKEIARTEKELRTVKAK
                     LGDKSFVERAPTAVVEEHRRRLKDFSAQLAKLKRAREGLN"
BASE COUNT         1146 a         1448 c         1340 g         1071 t
ORIGIN      
        1 acaatggagc gcgaacgttt tttacaatct cggcaatgct tattttcgcg cgggcgattt
       61 tgggcgcgcg atcctgaatt atgagcgagc cctcgccttg gaacgacatc atcccgaagc
      121 gacggccaat ttgcagatcg cgcgcgacga ggcgcgcgcg ctcgagatgc aaccgagctg
      181 gccggaacgt tatctgcagt ttgcgagcat caatcaatac agcgtcgcag gggcgattgc
      241 cttttggatc gcactgtttt gtgtggtcgg attgattttc gggcgccgtc gatcagccgc
      301 cataatcgct ttatcgatct tttcgctcct agtctttgtc gtcgcggttt tagcgattta
      361 tgaactcgac catggcagca agggccgcgc actcgcgatc gtgacgggca aagacgtgca
      421 ggcacgcgtt gcgacggcgg acactgcaaa cagcgtcctg gtattacctc ctggaagcga
      481 ggtcaaaatc ctgaacgctc gtggcgactg ggtctacgcc gccctgccga ataatttgcg
      541 cggctggatt ccggcgaaaa gcgcggagca agtgcgcctc tgaccaaggc gcgtgatcgc
      601 gcgaagtctt gggagcgcac gcgccatcgc gtgctacgat tggcgccctc gccggttgct
      661 gggaaaacta cacgcgaggc gcgtgcgctc cccagaacag cgccgcggac gcccgcaagc
      721 gaatttcaat agtgaaatcg tcgcagcact tttatgcttt ccaaaaccaa atgaaggtcg
      781 ccgttgctca gatttcgtgt tcgctcggta atcccgacgc aaatcttatc aagacccgcg
      841 atttttccgc gcgcgccagg gacgctggcg ctgagctgat tgtttttccg gagatgattg
      901 ataccggcta ctcgatgccg gtcattcaaa cgcacgctgc gccgtggacc agcggattcg
      961 tgcctggact tcaagaaatc gcccgggagc tttcgattgc gatcgtcagc ggcgtatcgg
     1021 agcgagatgg tgccctgatt tacaattccc agatttttgt tgatcaacag ggcaacatca
     1081 ttgcgaaata tcgtaagact cacctgtacg cagtcgcgcc tgtcgaagaa cagaagtgct
     1141 ttgctcacgg cgatgcgttc acggactttg ccattggcgg tctgcgcttt ggtttcagca
     1201 tctgctacga ccttcggttt ccggaggtgt atcgaaaact ggtcgccgac caaaacgtcg
     1261 gtgctttcat cgtctcttct gcctggcctt tcccgcgcct cgaacatttt cggatccttt
     1321 cggtcgcgcg tgcgatcgaa aaccaaagtt atgtgattgc atcgaatcgc gttggtaaag
     1381 acgatgactt gtggttctgc ggtagctccg caatcatcga tcctcgcgga atcgtgattg
     1441 cagccgcatc ggcagaccgc gaggaactta ttcaggccga cttgtccgaa gagcttgtgc
     1501 tctcagtccg gcgccgggtg gagtcgttcg cgcatcgccg gaagaatttg tataaataaa
     1561 aatgacgaag ttcccgcctc cgctgcgcta cggcgcggca ggcgaataat gaatggctaa
     1621 aactccgcga gttgcgcggc actgattggg acgaattcgc tttcgtgcag aactactcgc
     1681 ctggtgccac ggagaagctg ccaccattgt cttgccgacc cgattacaat caggagcacg
     1741 agcacgagaa agaaaagggc aaccaccgcg tcgaaccgcc agacgcctgc ctgccgcgct
     1801 aaatcagccg ctttggctgg gtcggcaatt ccggctgcct cgtgcataag cgagcgcgcg
     1861 ccgctcaaga aaccaagtct tggatcaggc gaaaagacct tcaagtaacc agccgagaat
     1921 gtaacgacgc acatgaacac aagcggcaaa acggtcacga aaagatattt cgccttatgc
     1981 attttgatca gaatcgttgt gccaagacag agcgcgacta ccgagagcaa ttggttggcg
     2041 agtccgaaaa gcggccagag cgagttgatg ccaccgagag gatcgatgac cccgacgtac
     2101 aaaaaccagc cccacgccgc gaccagcaac acactggaga aaacgttcgc gctccacgag
     2161 cgcgtgttgc cgagtggacg ccagatagtg cccaggaaat cttgaagcaa aaatcgtccc
     2221 actcgcgttc cagcatcaat cgtcgtcagg ataaacagcg cctcgaacat gatggcaaag
     2281 tggtaccaca atcctagcgc ggttgggctg gccgaaacgc gagcaaacat atgcgccatg
     2341 ccgacggcaa aagtcggcgc gcccccggcg cgattgaaca tggtggactc acccagattc
     2401 gtggcgagcg tctgcatttc cgtttccgtt acgggaaatc cggaagcaga aactctcgcg
     2461 accacttccg caggcgttcc ttttgtatta atcgcaaagt attcgcccgg ttgcagcacg
     2521 cacgccgcga tcatcgccat caaagcgacc atcatttcgg tcaccatcgc gccataaccg
     2581 atagtgcgta ttcgcgattc gcgtctcacc attttcggcg ttgtgcctga ggcaatgaga
     2641 gaatgaaacc ctgagacggc gccgcaggcg attgtgatgc agacaaaggg gaagactgga
     2701 ccggcaaaga cgaggccgct gccgtcgatg aattttgtga tcgccggcat ttgcaatgtc
     2761 ggattgatca gcacgacagc cgccgcgagc atgacaaccg tgccgagctt caaaaacgtg
     2821 ctcagataat cgcgcggcgt gagcagcatc cacaccggca gaatcgacgc cgccagacca
     2881 taaatcatga tcgcccatgc cagccatttc tggtcgtgtc gaaaccaggc ttcaatcgca
     2941 ggaaagtccg cgagaaattg tccgccccag acgccaaacg ccaatccgag caatccgaaa
     3001 atcgtgactg ccatcacact cacttttccc gtgcgcaaac cgattcccat gattagtgcc
     3061 accggtatgg tcatggcgat ggtgaaaacg ccccacggac tttctgccag ggcttgcacc
     3121 acgacgagcg caagcacggc cagcaaaata atcatgatcg ccaacacact gatcagggcg
     3181 agcgcaccga ccccgcgacc gatctcttct ttcaccattt gaccgagcgt tttgccgccc
     3241 cgccgaatcg atgcgaaaag catgatcatg tcgtgcacgc cgccgccgag tgtcgcaccg
     3301 atcaagatcc agagcgtgcc cggaaggaaa ccaaactgcg ctgcgagaac aggccctacc
     3361 agcgggccag gaccggcgat tgcggcaaaa tgatgcccga aaaccatcca gcgattcgtc
     3421 ggcacataat ctttgctgtc gttctggaca accgctggcg tggcccgctc atcattaagc
     3481 atcagaactt ttgccgccag ccatttgctg taaaaccggt agctgataga aagtgcgcaa
     3541 aggcccgcga taatgatcca tagcgcgctg atttgctcgc cccgcgaaat ccccaaaatc
     3601 gcaatggccc ccacccccaa cgcagagacg gcaaaccaga gcagcttttt ccagagactc
     3661 atctcgagtg cagtttaatt gaaattattg cggtgtgcta caaggtgtga tgtgcaactc
     3721 agccacaaga taaagaagct tctgggcggc cagggaatag ccagtgcgag aaagcacacg
     3781 ctccggtcgc ttcgcgcgaa ccgatttccg ctcgagaaaa aacgcgttat tgaaacaatc
     3841 gaccccgcag ctttcgagca aattcgccgg cgttacgcgg tcaacaagcc cacggacgat
     3901 tggcggaaat atctcgatct cgaccggtgg atcgccatca atattcgtcg cgttcgtgac
     3961 atcgagctcg atttggcgcg cccgaagcgc atcctcgacc ttggctgcgg cgcaggatat
     4021 tttctctaca tcgcccaatt gctcgggcat agcggacttg gtctcgattt ggatcgccag
     4081 cccatgtttg cagaaatcac ccgtctcctc ggcgttcatc gcgtgatcca gcgagtccaa
     4141 gcgtttcatc cactccctgc tctgggagaa aaattcgatc tcatcaccgc ctttatgatt
     4201 tgcttcaaca accaccgacg gcccagtctt tgggaagtag ccgagtggaa ttttttcctc
     4261 gatgacattg ccaaacatct ggctccacgc gggcgcgtct ggctcgagtt gaaccgcgag
     4321 accgacggga ccctttacac gccggaacta aaggagttct tccagaaacg tggggccagg
     4381 atcgatgagc acaaagtcat gtttaattca ggccttctcg cgcccgcttc aacttcgcca
     4441 gttgcgcgct gaaatctttt aaccggcgtc gatgctcctc aacaaccgcg gtgggcgcgc
     4501 gctcgacaaa cgatttgtct ccgagcttgg ccttgacggt tcgcaattct ttttcggttc
     4561 ttgcgatttc cttgtccaac cgcccgcttt ctgctgcctt gtcggccgcc gcggttgcta
     4621 acaaaatctc tcctaatggg gtgaccgcga ctgggctgcc aggttgcgct tcatattgtt
     4681 ggtccagctt tagttctgcg gcatttaaca atcgtgagat cgtgggaatt tcgtctgaaa
     4741 ttgctttctc atctgtccgt aaaatgaaat gtatcttcct gttggatggg agcttggctt
     4801 ccgcccgcaa attgcggccc gcctgaaccg ttttgtaaat gtccaagaca aactgacgct
     4861 tgcccgcgac atcaaccttg tccaaaggca ttcgctccgg cagcggtgcg aactgaattg
     4921 tgtcttttcc aaaaccgagc agcgaccaaa gctcttctgt gatgtgcggc atgaatggat
     4981 gaagcaaccg caagactgct gagag
//
LOCUS       DASQUQ010000005         8842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1082148, whole genome shotgun sequence.
ACCESSION   DASQUQ010000005 DASQUQ010000000
VERSION     DASQUQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082148"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1102
                     /gene="egtD"
                     /locus_tag="VE486_00100"
     CDS             110..1102
                     /gene="egtD"
                     /locus_tag="VE486_00100"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZA37516.1"
                     /translation="MTANRLTITRPPAGVASASPNFLSEVIAGLSSKPRTLPCKYFYD
                     ERGAALFQKICELPEYYITRTEIDILDRYRAEIAAHLGPEISLIGLGTGSGTKTRILI
                     EALEKPAVYIPVDISEKQLRKSTARFRKIFPDLEIQPVCADYLQPVVLPPTRHQAARN
                     VVYFPGSTIGNFEPDEATEFLRRIAKVCRHNGALLIGVDLRKDRDVIEAAYNDTAGVT
                     AQFNLNLLVRANHELGADFNLQHWRHRAIYNSEAGRIEMHLISQIDQAVHIADYTFGF
                     RSGEKIVTEFSYKYAPEGFAALARKAGFEFQKIWTDDARLFGVFYFTCSPDTSG"
     gene            1136..1879
                     /locus_tag="VE486_00105"
     CDS             1136..1879
                     /locus_tag="VE486_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZA37517.1"
                     /translation="MSALVQLVDVSKRYANAPALHSTNLAIERGKTTVLIGPSGCGKS
                     TLLRLIIGLITPDSGTITFNGSQITPANIDTLRPRIGYVIQEGGLFPHLTARDNILLM
                     SRYLRKSREKLRTRLLELCELTRFPDNLLSRYPIELSGGQRQRVSLMRALMLSPELLL
                     LDEPLGALDPLVRASLQKELKEIFTRLRQTAFFVTHDLAEAAYFGDEIVLMNEGHIVQ
                     QGSLRDLREKPANDFVREFINSQRALAFT"
     gene            1876..3405
                     /locus_tag="VE486_00110"
     CDS             1876..3405
                     /locus_tag="VE486_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZA37518.1"
                     /translation="MKLLPRPRLVGRDRRARRGRPSVLFLPFLVLFLSIFSANANPVV
                     IGSKKFTESYVLGEIAKRTLNDAGIPAEHRQGMGGTIILWQALRGGQVDAYPEYTGTI
                     AEQILKTDRELPLDEIRDALTKFDVGMTKPLGFNNTYALVMRRSEAERLGLRTITDLR
                     NHPELKIGLTHEFLDRQDGWQPLRARYGLPQQNVTGIDHALGYAALNNGSIDVKDAYS
                     TDAKIAEYDLVALDDDLQFFPRYDAVFLYRVSLPESTITVLRKLEGMLDQTRMLRLNA
                     EAERTKDYGKAANLYFQDRAGSPFAGGESFWHKLGRWTLRHLELAGFSLLLAVIAGVP
                     LGIVASRGGPAGHAILGFASVVQTIPSLALLALLVPIPFFGISVRTAIAALFLYGLLP
                     IVRNTATGLQDIPRGLRESAIALGLSPMARLWQIYMPMASRSILAGIKTSAVINVGTA
                     TLAALIGAGGLGEPILSGLNLNDHVTILEGAIPAALLALLVQWGFDFLDRVLIPKGLR
                     I"
     gene            3460..4824
                     /locus_tag="VE486_00115"
     CDS             3460..4824
                     /locus_tag="VE486_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZA37519.1"
                     /translation="MAIASVNPATGEKLKEFPAFSDSEIEKRLKQAERVFAHHRRRPF
                     AKRAQLMMATAGLLDQEKDKLALTITLEMGKLFRAAQDEVVKCARACRFFAENSERFL
                     EDEVAQTDAARSYVRYEPLGAVLAIMPWNFPFWQVLRFAAPALMAGNVGLLKHASNVP
                     QCALEIERLFCRAGFEDGVFQTLLIEPNQVEKVIIDTRVKAVTLTGSEKAGSAVASTA
                     ARQIKKSVLELGGSDAFIVMPSADFESALSTAIKARTINTGQSCIAAKRLFIADNIYD
                     EFLKQFVERTRALKVGDPMDETTEIGPLATEQILQGVHEQVQKSIAAGAKLLTGGNRI
                     HGPGFFYEPTVLVDVPKGSPAAREEVFGPVASIFRVRDAADAIEMANDSTFGLGASAW
                     TNDSAEQELFSSELETGMVFINAMVASDPRLPFGGVKRSGFGRELGAAGIREFTNPKT
                     IWID"
     gene            complement(4895..6154)
                     /gene="fahA"
                     /locus_tag="VE486_00120"
     CDS             complement(4895..6154)
                     /gene="fahA"
                     /locus_tag="VE486_00120"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HZA37520.1"
                     /translation="MPLRSFIEIAKDSHFPLENLPFGVFQPKQAKPRVGVAIGDLIVD
                     LSVLEELGHFRLPEFQNQQVFSEESLNAFLALGRRAWRKAREVLQHLLSAETPTLRND
                     ANLRARVFHAQNDVTMKLPARIGNYTDFYSSYHHAHNVGTMLRGPENALMPNWKWLPV
                     AYHGRASSVVISGTDVRRPSGQIKPPEAAAPIFSQAKSLDFELEMAFLIGPGNSLGEP
                     VSIDRAIDHIFGFVLMNDWSARDIQAWEYQPLGPFLAKNFCTSISPWVVTAEALEPFR
                     KPLPPQDPKPLPYLRGKNDFMFEIQLEAYLQTTAMDSPAVITRTNFQNLYWSVAQQLA
                     HHTVNGCNLQPGDLLASGTISGPTEESRGCMLELTWRGANPLKLPNGETRKWLEDGDK
                     LVITGWSQGDGYRVGFGEVSGRIVPGG"
     gene            complement(6245..7159)
                     /locus_tag="VE486_00125"
     CDS             complement(6245..7159)
                     /locus_tag="VE486_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37521.1"
                     /translation="MPAASLQALLEQSIDYAGMFPPCSLVLEPALRNQATYVRSDDAW
                     MLNTFVLPVTQFDSAKQLLPAFDPSHPLRVSALGPKTESAAAFREAFAETEEAIRSLA
                     AHNVDLVSVNQLEMWLPEGVDFSVLTEARAIIGNLPAFWEAPAERARETITLLAEINS
                     EIDEPTFGFKLRTGGVTPDAFPTSAQIARALVVPATHQVPIKFTAGLHHPFRQYREEV
                     QTRMHGFLNVLGAAILAAEYRWDEKQTAAMLEDEDAKSFSFDDEFFAWREWKIDIERL
                     NDRRKFVTSFGSCSFDEPREDLHVLGLL"
     gene            complement(7257..8519)
                     /locus_tag="VE486_00130"
     CDS             complement(7257..8519)
                     /locus_tag="VE486_00130"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZA37522.1"
                     /translation="MTIYDDEVFKMACDQFKVIADYLNIDQDDREWATYPKRAVAVTL
                     PVHMDDGSTRAFQGYRVQHHIALGPTKGGTRFAPSLSMGETAALAMWMSWKCALANLP
                     YGGAKGGVAIDPSKLSHTELEAVSRRYMQEMIPFVGPHTDIMGPDMGTNEQIMAWFMD
                     TYSVYMGYAVNEIVTGKPVAIGGTEGRREATGRGVVYLVERAMNMLKMNPEKCTAIVQ
                     GFGNVGSVAALSLAYKSGVKIVGISDAYTAIYNPKGIDVKAAEQHVLKKRHLKAFDQA
                     NHVDPNELLTMKCDVLIPAAVDRVITEKNAAKLQCRILAEGANGPTTPAADRILEQRW
                     DEVFVIPDILCNAGGVIVSYFEWVQGLQSFLWTEMEVTDKLFRILEHSFAQVIRRAKE
                     HKVSHRTAAMAIGVERVIKAKQVRGLFP"
BASE COUNT         2007 a         2454 c         2400 g         1981 t
ORIGIN      
        1 tccagcctga gaaacgctgg caattcaccg gaattcggct ggcacgcgat tcacagtaag
       61 cggcggctcg ctggtcgcca tcacgatcgc gccgaaccaa catcccgtaa tgacagcgaa
      121 ccgactcaca attacgcgtc ctccagccgg tgttgcatcg gcaagcccta atttcctgtc
      181 agaagtaatc gcgggtcttt ccagcaaacc gcggacgttg ccatgtaagt atttttacga
      241 tgagcgcggt gcggcgttgt tccagaaaat ttgcgagctg ccggagtatt acattacgcg
      301 gaccgagatc gatattctgg atcgatatcg cgccgaaatc gcagcccatc tcggcccaga
      361 aatcagcctg attgggctcg gcaccggctc gggcaccaaa acacggatcc tgatcgaagc
      421 cctcgaaaaa ccggccgttt atatcccggt ggatatttct gaaaaacaac tgcgcaaatc
      481 gacagctcgt ttccgtaaaa tttttcccga tctggaaatc cagcctgtgt gcgccgatta
      541 cttacagcct gtcgtactcc ctcccactcg ccaccaagcg gcgcgcaacg tggtctattt
      601 ccccggatca accattggca atttcgagcc tgacgaagca actgagttcc tccgccgaat
      661 cgctaaagtt tgccgccaca atggcgcact actcatcggc gttgatcttc gcaaagatcg
      721 ggatgtgatc gaagctgctt ataacgatac cgccggcgtc acagcgcaat tcaatctgaa
      781 cctgctggta cgcgcaaacc acgaactcgg cgcggatttc aatctgcaac attggcggca
      841 ccgggcaatt tacaactcag aggccggccg catcgaaatg catctcatca gtcaaatcga
      901 tcaagctgta cacatcgccg attacacatt cgggtttcgt tccggtgaaa agatcgtcac
      961 ggaattctcc tataaatacg ccccggaagg tttcgccgcc ctcgcgcgca aagctggctt
     1021 cgagtttcaa aagatatgga cagacgatgc gcgtctgttt ggtgttttct atttcacatg
     1081 tagtcccgat acgtcgggat gaccccgctg cggtcaatgc ccgcggctac aagaaatgag
     1141 cgcgctggtt caactggtcg atgttagcaa gcgctatgcg aacgcgcccg cgttacattc
     1201 cacaaatttg gcgatcgagc gcggcaagac cacggtattg atcggtccga gcggttgcgg
     1261 caaatcgaca ttgctgcggc tcattatcgg attaatcacg cctgattctg ggaccatcac
     1321 tttcaatgga tcacagatca cgccggctaa catcgatacg ttgcgtcccc gaattggtta
     1381 cgtcattcag gaaggcggtc tattcccaca tctgacagcg cgcgacaaca ttctcttaat
     1441 gtcacggtat ctgagaaaat cccgcgagaa attgcgcaca aggcttctcg aattatgcga
     1501 gctgacaaga tttcccgaca atttgctctc gcgatacccc atcgaacttt cgggcggtca
     1561 gcgtcaacgt gtcagtttaa tgcgcgcatt gatgctttcg cccgaactgc ttctcctcga
     1621 cgaaccgctg ggcgcgctcg acccattggt gcgcgcttcc ttgcaaaagg agctcaaaga
     1681 aattttcacg cgcctgcgtc aaacagcgtt ctttgtcacc cacgatttgg cggaagcagc
     1741 gtatttcggc gatgaaatcg tgctcatgaa cgaaggccac atagtccaac aaggttcgct
     1801 tcgcgatctt cgcgaaaagc cagcaaatga tttcgtacgc gaattcatca attcgcagcg
     1861 ggcgcttgcc ttcacatgaa actattgcct cgcccaagat tggtagggcg cgaccgccgg
     1921 gcgcgccgag gacggcccag cgttctgttc ctgccatttt tagttctgtt cctttcaatc
     1981 ttttctgcga atgcaaatcc cgtcgttatc ggttcgaaaa aatttaccga atcctatgtg
     2041 ctcggtgaaa ttgcgaagcg cacgctaaat gatgccggca ttcccgcaga gcaccgtcag
     2101 ggcatgggcg ggacgatcat tctttggcag gcgttgcgtg gtgggcaggt tgacgcttac
     2161 ccggaataca caggaacgat cgcggagcag attctgaaaa cggaccgtga attaccgctg
     2221 gacgaaattc gcgacgcgct cacgaagttc gacgtgggca tgacgaaacc gctcgggttc
     2281 aacaatacct acgcgcttgt gatgcgccgc agtgaagcgg agcgactcgg tctccgcaca
     2341 atcaccgatc tgcgaaatca tccggagcta aaaatcgggc ttacccatga atttctggat
     2401 cggcaggatg gatggcagcc attacgcgcg cgttatgggc ttccgcaaca gaatgtgacc
     2461 ggtatcgatc acgcgctcgg ttacgcggcg ctcaacaatg gctcgatcga tgtgaaagat
     2521 gcctactcga ccgatgcaaa gattgcggaa tacgatctcg tcgcactgga cgatgacctg
     2581 caattttttc cgcgatacga tgcagtgttc ttatatcggg tttcgttgcc agaatcgacc
     2641 atcacggtac tgcgcaaact cgaaggaatg ctggaccaaa cgcgcatgct ccgactgaac
     2701 gccgaagcgg aacgcacaaa ggattatggg aaagccgcga acctgtattt tcaagacaga
     2761 gctggatcgc catttgccgg tggcgaatca ttttggcaca agcttgggcg ctggacgttg
     2821 cgccatttgg aacttgcggg gttctcgctg ctgctggccg ttatcgccgg tgtcccgctc
     2881 ggcatcgtcg ccagccgggg cggtccggcc ggccacgcta ttctcggttt cgcgagtgta
     2941 gtgcaaacga ttccatcgct cgctttgctg gcgttacttg taccgatccc gttttttggg
     3001 atcagcgtcc gcaccgcgat tgccgcgctt tttctttacg gattactgcc aattgtgcgc
     3061 aacaccgcaa ccggcttgca ggatattccg cgagggttgc gcgaatcagc aattgcactg
     3121 ggactcagcc ccatggcgcg gctgtggcaa atttatatgc cgatggcatc gcgctccatt
     3181 ttggctggaa tcaaaaccag cgccgtgatc aacgtcggaa cggcgaccct ggcggcgctc
     3241 attggagcgg gcggtctggg tgagccgatc ttgagcggac tgaatttaaa cgatcacgta
     3301 acgatcctgg agggcgcgat cccagcagcc ttgctcgcat tgctggtgca atggggtttc
     3361 gattttctcg atcgcgtttt gattccgaaa ggcctgcgaa tctagcgcgc tgccagaaga
     3421 aaagtatgcg gcggtcatag accgccgcta cagtgttgaa tggccattgc gtcggtcaac
     3481 ccagccacag gcgaaaagct gaaggagttt ccagctttta gcgacagcga aatcgagaaa
     3541 cgcctcaagc aagccgagcg cgtgtttgcg catcatcggc gacgcccatt cgccaagcgc
     3601 gcgcaactca tgatggccac agcgggattg ctcgaccagg agaaggataa gctcgctcta
     3661 acaattaccc tggagatggg aaaattgttc cgtgctgcgc aggatgaggt cgtgaaatgc
     3721 gcccgagcct gtcgcttttt tgccgagaat tcagagcgtt ttctcgaaga cgaagtcgcg
     3781 cagactgatg ctgcgcgcag ttacgtgcgc tacgaaccac tcggcgccgt cctggccatt
     3841 atgccatgga attttccgtt ctggcaggtg cttcggtttg ccgcgccggc gctgatggca
     3901 ggcaatgttg gactgctcaa gcatgcgtcg aacgttccgc agtgtgcttt ggagattgaa
     3961 cgactctttt gccgcgcggg atttgaagat ggcgtctttc agacgctgct catcgagccg
     4021 aaccaggtcg aaaaggtaat tatcgataca cgtgttaaag cggtgacgtt gacgggcagc
     4081 gaaaaagcgg gaagcgcggt agcaagcacg gctgcgcgcc agatcaagaa aagcgtcctc
     4141 gaactcggcg gcagcgacgc attcatcgtc atgccaagcg cagatttcga gagcgcactg
     4201 agcaccgcga tcaaagcccg cacgatcaac accgggcaat cgtgcatcgc ggcaaagcga
     4261 cttttcatcg cggacaacat ttacgacgag tttctcaagc aatttgtcga gcggacacgc
     4321 gcgctgaagg tcggagaccc catggatgag acgactgaga ttgggcccct cgctacggaa
     4381 cagattttac agggggttca cgagcaggtt cagaaatcga tcgcagcagg cgcaaaatta
     4441 ctgacagggg gcaatcgtat tcatggcccc ggcttttttt acgagccgac tgttcttgtc
     4501 gatgtgccga aaggatcgcc agccgctcgt gaggaggttt tcggcccggt ggcgtcgatt
     4561 ttccgggtac gagacgcagc cgacgcaatc gagatggcaa acgacagcac attcggcctc
     4621 ggcgcgagcg cgtggacgaa cgattccgcc gagcaagaac tcttcagctc cgagctggaa
     4681 acgggaatgg ttttcatcaa tgcaatggtc gcgtccgatc cgcgtctgcc gtttggtgga
     4741 gtaaaacgct ccggatttgg gagagagctc ggcgctgccg gaattcgcga attcacgaat
     4801 ccaaaaacga tttggattga ctaacggcag cgcgtgcggg catgctgacg aatgcgttcc
     4861 ccgccgagcg gcgaaccttt gttgtgacag cagtttaccc gccaggcacg atgcgtccac
     4921 ttacttcgcc aaaacccact cggtagccgt cgccctgact ccagccggtg atcacaagct
     4981 tgtcgccgtc ttcgagccat ttgcgcgttt cgccattggg cagctttaac ggattggctc
     5041 cgcgccatgt cagctccagc atgcagccgc gcgattcttc cgttggtccg ctgattgtgc
     5101 cgctcgccag caaatcaccg ggttgcaaat tgcacccgtt aacagtgtga tgggcaagtt
     5161 gctgcgcaac gctccagtaa agattttgaa aatttgtgcg tgtgataacg gccggcgaat
     5221 ccatcgcggt agtttgcaga tacgcttcga gttggatctc gaacatgaag tcatttttcc
     5281 cacgcaaata cggtagcggc ttggggtcct gcggtggcag cggtttgcga aaaggctcaa
     5341 gtgcttctgc cgttaccacc cacggtgaaa tgctggtgca aaaattcttg gccaggaacg
     5401 gcccgagcgg ctgatattcc cacgcttgaa tgtcgcgcgc actccaatcg ttcatcaaga
     5461 cgaagccgaa gatatgatca atcgcgcgat cgattgaaac cggttcacca agcgagttcc
     5521 caggtccgat caggaacgcc atttccagtt cgaaatcgag gcttttagcc tggctgaaaa
     5581 ttggggccgc tgcctcgggt ggtttgattt gtccgcttgg tcgtcgcacg tcggtgccgc
     5641 tgatgacaac ggagctggcg cgcccgtgat aggccacggg caaccatttc caattcggca
     5701 tcagggcgtt ttcgggccca cgtaacattg ttccaacatt atgggcatgg tgataggacg
     5761 aatagaaatc ggtgtaattg ccaatgcgcg ccggcaattt catggtgaca tcgttttgcg
     5821 cgtgaaaaac gcgggcgcga agatttgcgt cattacgcag agtcggcgtt tcggcggaca
     5881 aaagatgctg aagcacttcg cgcgctttgc gccacgcccg ccggcccagc gcgagaaagg
     5941 cgttgagtga ttcttccgaa aagacctgtt gattctgaaa ctccggtaag cggaaatgac
     6001 cgagctcttc gagaacagaa agatcgacga ttagatcgcc aatcgcgact ccgacacgtg
     6061 gtttagcctg tttcggttgg aaaacgccaa atggcagatt ctccagcgga aaatgcgaat
     6121 cttttgcgat ttcgataaag gaacgaagag gcatgcttac gatttaacca cgaagatcac
     6181 agaggacacg aagagtttta atcctctttt aaaggcttcg tgatcttcgt gtccttcgtg
     6241 gtgatcaaag aagaccaaga acgtgtaaat cttcgcgtgg ctcatcgaag ctgcagctgc
     6301 caaaagaggt cacgaatttc cgcctgtcat tcagccgctc gatgtcgatc ttccattcac
     6361 gccaggcaaa aaattcgtca tcgaacgaaa atgatttcgc atcctcgtcc tccagcattg
     6421 cagcagtctg tttttcgtcc cacctatatt cagctgccag aatggccgcg cccagcacgt
     6481 tgagaaatcc gtgcatccta gtttgcactt cttcgcggta ctgcctgaac ggatgatgca
     6541 gaccggccgt aaatttgatc gggacttgat gcgtcgccgg cacgaccagc gcccgggcga
     6601 tctgggctga ggttggaaat gcgtccggcg ttacgccgcc ggtgcgcagt ttgaaaccaa
     6661 aagtcggctc atcgatttcg gaattgatct cggccaacaa cgtaatcgtc tctcgcgctc
     6721 tttcggccgg cgcctcccaa aacgccggca aattgccgat aatcgcgcgg gcttctgtaa
     6781 ggacagaaaa atcgacacct tccggaagcc acatttccaa ttgattgacc gaaacaagat
     6841 cgacattgtg cgcagccagc gaacgaatgg cctcctcggt ttctgcgaag gcttcacgaa
     6901 aagctgccgc gctctcggtc ttcggcccca gcgcagaaac ccgtagcgga tgggacggat
     6961 cgaacgccgg aagcaattgt ttagccgagt cgaattgtgt gactggcaaa acaaacgtgt
     7021 tcagcatcca ggcatcatcg gatcggacat atgtggcctg attccgcagc gcgggttcca
     7081 gcacgaggct gcaaggcggg aacattcctg cataatcaat tgactgctcg agcaaggcct
     7141 gaagcgaagc ggctggcata cccgattcaa tggtgcaaca aggcgcttcg tcaaaccgct
     7201 agttgcgaaa gcttaaattc actttccccg ggataggact ccgactttac cgcgaatcac
     7261 ggaaacaagc cgcgcacttg tttggctttg atgacgcgct cgacgccgat cgccatggcc
     7321 gcggtgcgat gcgaaacctt gtgttccttc gctcgcctga ttacttgcgc aaacgagtgc
     7381 tcaaggatgc ggaaaagttt gtcggtcact tccatttctg tccaaagaaa tgattgcaga
     7441 ccctgcaccc actcgaagta ggaaacgatc acaccgcccg cgttgcacag aatatcgggt
     7501 ataacgaata cttcatccca tcgctgctca agaatccgat ctgccgcagg cgtggtcggg
     7561 ccgttggcgc cttctgccaa aatgcggcat tgcaacttcg cagcattttt ttccgtaata
     7621 acgcgatcga ccgcagctgg aatcagcaca tcgcatttca tggtgagcag ctcgttcgga
     7681 tcgacatggt tcgcctggtc gaacgctttc aggtgtcgct tcttgaggac atgctgctcg
     7741 gcggctttta cgtcgattcc ttttgggttg taaatcgcag tgtacgcatc gctgatccca
     7801 acgatcttga cgccgctctt gtacgcgagc gagagggcag cgacggaacc gacattgccg
     7861 aacccctgca caattgccgt acacttctcg ggattcattt tcaacatgtt catggcgcgc
     7921 tcgaccaagt aaacaactcc acgcccggtg gcttctcgcc ggccttcggt gccgccgata
     7981 gcgaccggtt ttccggtaac gatttcattc accgcgtagc ccatgtaaac ggagtaagtg
     8041 tccatgaacc acgccatgat ctgttcgtta gtccccatgt caggacccat gatgtcggtg
     8101 tgcggaccga cgaatggaat catctcttgc atgtaacggc gggagaccgc ttcgagttca
     8161 gtgtgggaca gtttggacgg gtcgatcgca actccgcctt tcgcgccgcc gtagggcaga
     8221 ttggccagcg cgcatttcca gctcatccac atcgccagtg ccgcggtttc gcccatcgac
     8281 aatgaaggtg cgaagcgcgt gccgcccttt gtaggcccga gcgcgatatg atgctggacg
     8341 cgataaccct ggaaggctct tgtgctgccg tcatccatgt gcacaggcag ggtgaccgct
     8401 acggcgcgct tgggatacgt cgcccattca cgatcgtcct gatcaatatt caggtaatcg
     8461 gcgatgacct tgaattggtc gcacgccatt ttgaacactt cgtcgtcgta gatagtcatg
     8521 ggaatgcgtt cgaaagttag gacatccggc cgcaaatgca gcttgctatt tgcgtcgggc
     8581 ggatagagcc gtcaactccg aaagctttcg cgagtcaaat cgaaaacatt aggacaagat
     8641 cgacaacgac atccggaatc cgtagcgttc ttttccgaaa tccatcaaaa atgaagattg
     8701 aaactttagc cgtccacgcc gggcatacca ttgatcctgc aaccggggca gtttcggcgc
     8761 ccatttatct ttccacgact ttcgagcgcg acatcgaagg cacttactcg cgcggattca
     8821 tgtacacgcg caacaacaat cc
//
LOCUS       DASQUQ010000006        11094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_541694, whole genome shotgun sequence.
ACCESSION   DASQUQ010000006 DASQUQ010000000
VERSION     DASQUQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541694"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="VE486_00135"
     CDS             <1..1528
                     /locus_tag="VE486_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37523.1"
                     /translation="RAQIARLLPKDPDSQLNLASAALRFGKIDLARKALNDVLPDYRG
                     SAAFHVVAGWLARAEGNLPEQEEQFAAAVKEEPGNDLYQFNLAALQLQSSDTQKSARA
                     RATLDRLSKVAPFRTGALRALLNDAVARNDLAAADNFAQQLQMSQEVTFGDYLLCLNF
                     YRKLDEKKFRPLLERVKPFAVRNPSDLASLINWMNENGLAEDAVKWIDKLPKDELTFP
                     PVSIALSATYANTKNWSRLKRWTSTPANAGWGDSDYLRIAYQAIATRQSQSRNGGTAQ
                     TDFETLWRSAEQAARESRKVSGERELDLARLASKWNLKKESELLWLRVANNPPMRREA
                     LDALRRFYRTDDDLEKLYDTLQRLHESSPTEAPITADLARLGLNIERNTKESHDLAKE
                     AYDRTPNEPNCAITYAFSLYRLGRGAEALGIIQKLPRDQLRDPHAAVYVALLLAEAGQ
                     IEGAKEYIAVADAGKIYLEEKKLLDEAKSKLAAASATPSPAVSPVPAELTPTPTASPP
                     "
     gene            complement(1546..2370)
                     /locus_tag="VE486_00140"
     CDS             complement(1546..2370)
                     /locus_tag="VE486_00140"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HZA37524.1"
                     /translation="MTAKTVDTKAKRERDDVYRRSMEVLQDANIPFLIAGAYVVEVYA
                     GISRRTKDFDLYLRPRHVDAAIDALADAGYKTEKTFPHWLAKAGRPRLYIDLIFRAGN
                     GLCEVDDSWFERAQKEELLGLQVKLCAPEEMIWMKAYIMERERFDGADIAHILQSCAE
                     KLDWPHLVHRFGPDWRVLLSHLVLFGYIYPTEHGRIPAAIMDDLISRLRKDAPATGPE
                     RLCRGTLLSRKQYLMDVQKRGFRDARLEERAHIDAGDIAHWTKAIAKEEKARGKPA"
     gene            2537..3262
                     /locus_tag="VE486_00145"
     CDS             2537..3262
                     /locus_tag="VE486_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZA37525.1"
                     /translation="MKMAKRALRIAATADLHYAKHSRGMLHEAFAEISRTADVLLLCG
                     DLTDYGLPEEAEELVADIRATVRIPMLAVLGNHDFESGHEELVSKVLDDAGVNVLNGE
                     AIEIAGVGFAGIAGFGGGFGRRMLNAWGETLIKQFVQESISHAVRLEQALAKLQTERR
                     IAVLHYSPIRATLEGEDPEIFAFLGSSRLEEPINRFRVSAVFHGHAHNGILDGKTSTG
                     IPVYNVSAPALRKSGKPYRIVEL"
     gene            complement(3455..3979)
                     /locus_tag="VE486_00150"
     CDS             complement(3455..3979)
                     /locus_tag="VE486_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37526.1"
                     /translation="MKKTAITYWLIPADPARSFLERTIVDLARRYSAPVFEPHMTIYV
                     GLDRVDAEEVITKTVSGCRPVKAKALKICQSGEFIKTLFVQFALDRKLQQLNEMIRDA
                     AQDSSDYDLKPHLSLLYKKMSILARRRLAHSIKLPFSEVTFESIKAIRCASPTRNRAD
                     VEAWRVVATKALSG"
     gene            4063..4422
                     /locus_tag="VE486_00155"
     CDS             4063..4422
                     /locus_tag="VE486_00155"
                     /inference="COORDINATES: protein motif:HMM:NF021510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HZA37527.1"
                     /translation="MPTNVLGGELKCCCRNPVTGFYRDGFCRTGPEDVGLHTVCVKAT
                     QEFLDFSVLDGNDLVTPRPEWGFPGLKPGDKWCVCVTRWKAALDHNRAAPVDLEATHS
                     SALEFVTLEELKAHTVK"
     gene            4494..5786
                     /locus_tag="VE486_00160"
     CDS             4494..5786
                     /locus_tag="VE486_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA37528.1"
                     /translation="MKLPFSLFLALRYLKPKRTFLSIITLISVLGVMLGVTVLILVIS
                     VMTGFDRELRQKVIEFDAHILVTTEDVLRDWRTLKAKIDKTPGVVATAPYIQGPVIVE
                     FQNRRLAPLMRGVDPAEEEKVIPLKKFIKFGKLDLEGDSTVLGVELARKLQVNVGDKL
                     TVYSPGNLGQILDSIKKLENARGEDEKKAIDQLRDVILPKELTVTGIFETGHYLHDSE
                     FLLVPVYVGQELYGLGDALHGITVATDNPYSAERIKQAIQQFLEPPEYAQTWIDMNRQ
                     FFEAVRLERTVMFFLLFFIVVVAAFGIMSTLITVTVQKRREIGIMKALGANLAQIIWV
                     FLGQGTVVGLFGTLTGLGLGMTLIRYRNEFSRWLASTLHIEIFPREVYQFSAIPAEVI
                     PKDVAIICISAFFICSFAALIPAYFAARLDPVKALRYE"
     gene            complement(5789..6307)
                     /locus_tag="VE486_00165"
     CDS             complement(5789..6307)
                     /locus_tag="VE486_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HZA37529.1"
                     /translation="MKIISGGQTGVDRAALDVALKHGIECGGWCPGGHLDEFGRIPDR
                     YPVKELKHGGFTERTLQNVKDSAGTVIIYSGKLSGGTEQTVRFSVEQKRPHHLIDASK
                     ISAEDAGKSIANFIRKHKIDILNVAGPRQSEWPGGYDYALRALEAFVNSIASESTRLR
                     QATARQASRSKR"
     gene            complement(6322..6708)
                     /locus_tag="VE486_00170"
     CDS             complement(6322..6708)
                     /locus_tag="VE486_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZA37530.1"
                     /translation="MDSKKSRQDFPYKTYLNILHGPLELIDVQKLADACTDKWYNQTL
                     CKVNDSVVRMAVIQGEYHWHEHKDIDEFFFVLDGHFLIDLEDRVVDLAPRQGFVVPKG
                     VRHRTRAPERTVILMVERADIVPTGD"
     gene            complement(6814..7344)
                     /locus_tag="VE486_00175"
     CDS             complement(6814..7344)
                     /locus_tag="VE486_00175"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZA37531.1"
                     /translation="MTVRDVSTALDMTKMRKVNLNEIKEETWQSPGGKYAACFKGISQ
                     ALGRDPSSLDLAKRHPFDLEWNRVPPGKRAFPYHAHSAQWELYLVISGKGSVRHKDGT
                     TEVVPGDAFIFGPDEPHQLSNPGQEDFIYYVIADNPIGESCYYPDSGKWKVNKSSAAD
                     RVVVKGEETDYFDSEE"
     gene            complement(7366..7866)
                     /locus_tag="VE486_00180"
     CDS             complement(7366..7866)
                     /locus_tag="VE486_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZA37532.1"
                     /translation="MRKVNLKEIPEQERKSPKGKFYKFVKDISVALGREPQSLDLAKR
                     HPFDLAMVRIPKGKIYCPYHSHSAESELYLVVSGRGSVRDKSGSTTVIAGEAFFFQPG
                     EAHQIANAGDEEFVYYVIADNPRSGDATGDSCYYPDSGKWAVVKEGLEGMIVKGKETD
                     YFDGEE"
     gene            complement(7900..8154)
                     /locus_tag="VE486_00185"
     CDS             complement(7900..8154)
                     /locus_tag="VE486_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37533.1"
                     /translation="MNNHLWFDVIGWAGAAALLVAYAMVSSKKFEGDSIAYQSLNIIG
                     SLLLAVNTIFYRAYPSSFVNMIWIGIAVFSIATRRRCSSR"
     gene            complement(8184..9101)
                     /gene="ligD"
                     /locus_tag="VE486_00190"
     CDS             complement(8184..9101)
                     /gene="ligD"
                     /locus_tag="VE486_00190"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZA37534.1"
                     /translation="MPNKAELIVEDRKLAVSNLDKVLYPKVGFTKGHVIDYYIRIAPV
                     LLPHLKDRPLTMKRYPDGVEGEFFYEKNCPSHRPKWVKTAQVWSEGNNRIMNYCLAQD
                     LPTLVWAANLADLELHTSLSRKNNIERPTMMVFDLDPGLPADIIQCCQVGLWLREALG
                     KMKLKSFAKTSGSKGLQVYVPLNTAVTYDQTKDLSRALAQLLEHEHANLVTSNMSKAV
                     RKGKVFVDWSQNDEHKTTICAYSLRAREEPTVSTPVTWDEVANCLKKKKTDLLKCRSD
                     QALARVEKLGDLFEPVDKLKQGLPKKWKI"
     gene            complement(9163..10353)
                     /gene="lpxK"
                     /locus_tag="VE486_00195"
     CDS             complement(9163..10353)
                     /gene="lpxK"
                     /locus_tag="VE486_00195"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZA37535.1"
                     /translation="MGRRLENLEQFGIDVVLERRHGIRASILRGILHALSFVYERLVQ
                     LRLYFYRKRILRERALGCLVISVGNLTVGGTGKTPVVEKFARALQVGGRRVAILSRGY
                     KSVPRKRSWLDRLRGSMLDPPRIVSDGKSLLLDSRTAGDEPYMLAHNLKDVVVLVDKD
                     RVKSGLFAIDKWKIDTLLLDDGLQYLHLKHRLDIVLVDRQAPFGNEFLLPRGTLREAP
                     RNLRRASYIFITKTTGEPNDALVKRIRRYNRTAEIIECAHKPLYLKNLFTGERLPLER
                     LHDAFIGSICGIAAPESFEGALKKLGAHVDLAKRYIDHHYYTEAELTTFINRCIRRDL
                     AMIVTTEKDAVRMPHLPEAEVKVPICFLRVEIEILSGHESWEHCVARICKPQPMLSPE
                     RFFA"
     gene            complement(10355..>11094)
                     /locus_tag="VE486_00200"
     CDS             complement(10355..>11094)
                     /locus_tag="VE486_00200"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZA37536.1"
                     /translation="LVEEAADLFFRHTRRAPFSFSVSITGHIPPSRGLGSSVTVRLGV
                     LRALNALTGNRVDRLSIFQLCVQLEGHPDNAAPATFGGFTVVRGQTIQRFDVSARLSF
                     VLLIPDFKITTSGARRILPSQIKRVAAVENCANACAITAAFASGNYRDLRGAFADHLH
                     QPHRKKLIPFLPRVIAAAEKAGALGAFLSGSGSTICAVTLRSPKKIGTAMERSANSTP
                     ARIIITRADNHGARVFRIRNPQSTIRN"
BASE COUNT         2516 a         3098 c         2966 g         2514 t
ORIGIN      
        1 gcgcgcacaa atcgcgcggc tgcttccaaa ggatcccgac agccagctca acctggcttc
       61 cgcggccctg cgctttggga agatcgatct cgcacgaaaa gcgctgaatg atgttctccc
      121 cgactatcgt ggcagcgccg catttcacgt cgttgcgggc tggctggctc gcgcggaagg
      181 aaatcttccc gagcaggaag agcaattcgc cgccgctgta aaggaggagc cggggaacga
      241 tctttaccag ttcaatctcg cggcgctcca acttcagtcc agtgatacac aaaaaagcgc
      301 aagagcgcgc gccacgctgg atcgactcag taaagtcgct cctttccgca ccggcgcgtt
      361 gcgagcgttg ctcaacgacg ctgtggcgcg aaacgacctc gctgcggcgg ataacttcgc
      421 gcaacaactc caaatgtcgc aggaggtcac attcggcgat tacctccttt gcctgaattt
      481 ttatcgaaaa ctcgacgaga agaaattccg tcccttgctg gaaagagtga aaccttttgc
      541 cgtgcgcaat ccatccgatc tcgcgtcgct cataaactgg atgaatgaaa atggcctcgc
      601 cgaggatgct gttaagtgga tcgacaagtt accaaaagac gaacttactt ttccgcctgt
      661 ctccatcgcg ctttcggcca catatgccaa caccaaaaac tggtcgcgtc tgaaacgctg
      721 gacgagtact cccgcaaatg cgggatgggg cgactctgac tatttgcgca ttgcttacca
      781 ggccattgca acgcggcaat cacagtcccg caatggtggg accgcacaga ccgatttcga
      841 aacgctttgg cgttcagcgg aacaagcagc acgtgaatcc cgaaaggttt cgggagagcg
      901 cgagcttgat ctcgcgcgtc tcgcttcgaa atggaacctt aagaaagaat ccgaactact
      961 ctggctacgc gtcgcgaaca atccgcccat gcgccgcgag gccctcgatg cgctgcgccg
     1021 gttctatcgc acagatgacg atctcgaaaa actctatgac actctccagc gcctgcacga
     1081 aagctcgccc accgaggcgc cgattactgc agacctggct cgactcggcc tcaacattga
     1141 gcgaaataca aaagagtcgc atgatctggc caaagaggct tacgaccgca cgccaaacga
     1201 acccaactgc gccataacat acgcgttttc cctttatcgc cttggccgag gagccgaggc
     1261 gcttgggata atacaaaagc tgccacgaga ccagttacgc gatccgcacg cggccgttta
     1321 tgtggcgttg ttactggccg aggcagggca aatcgaagga gcaaaggagt acattgccgt
     1381 cgctgatgcc ggaaagattt acctggaaga aaagaaactg ctcgacgaag ccaaaagcaa
     1441 acttgcagca gcttccgcaa ctccatcgcc ggcagtctcg cctgtccctg ccgagctgac
     1501 tccgacgcca acggcttcgc caccttgaag cgtcgggttg ttaaatcacg cgggtttgcc
     1561 ccgcgctttc tcctcctttg cgatcgcctt ggtccaatgg gcgatgtcgc ctgcgtcaat
     1621 gtgcgcacgt tcctccaatc gcgcatcgcg aaatccccgt ttctggacgt ccatcagata
     1681 ttgctttcgt gagagcagcg tgccgcggca gaggcgctcg ggcccagtgg caggcgcatc
     1741 ctttcggagt cgtgagatca aatcatccat aatggcggcg gggattcgcc cgtgttcggt
     1801 agggtaaatg tacccgaaca acaccaagtg actcagcagc acgcgccaat cgggcccgaa
     1861 gcgatgaaca agatgcggcc aatccagttt ctcggcgcag ctctgaagaa tatgcgcaat
     1921 gtcagcgcca tcaaaccgtt cgcgctccat gatgtacgcc ttcatccaaa tcatctcctc
     1981 gggtgcacaa agttttacct gcaagccaag aagctcttct ttctgcgcgc gctcgaacca
     2041 ggaatcatcg acctcgcata acccatttcc ggcacgaaaa atcaggtcga tgtacagccg
     2101 gggccgtccg gctttggcca gccaatgtgg aaacgttttt tctgttttgt atccggcgtc
     2161 ggccagcgca tcgatggctg catcaacgtg gcgcggccga aggtatagat cgaaatcttt
     2221 cgtacggcgc gaaatacctg catagacctc gaccacgtac gcgcccgcga ttaaaaatgg
     2281 gatattcgca tcctgcagta cctccatcga tctgcgataa acgtcatcgc gttcccgttt
     2341 cgctttcgtg tctactgtct tggcggtcat tcaccaacat aacgcaatcg agcccgacat
     2401 cggctgcatt catgggcatt gccgctggcc cgagaacatg tccctggagg ggcgagctcc
     2461 ggcgagcccg cgcccgcggc gtcccagagc tccgctctcc aaaagcgtcc attggggccg
     2521 gcgatttcgt ttctcaatga agatggctaa gcgagcgcta cgaattgccg caactgcgga
     2581 tcttcattac gccaaacact cacgggggat gttgcacgaa gcgtttgccg agatctcgcg
     2641 cacggccgat gtgctgctgc tttgcggcga ccttactgat tatggacttc ccgaagaagc
     2701 agaggaactc gttgccgata ttcgcgctac ggtgagaatt ccgatgctgg cggtcctcgg
     2761 caatcacgat ttcgaatcgg gacacgagga gcttgtttct aaagttctcg atgacgcagg
     2821 tgtcaacgtg ctcaacggcg aagccatcga aattgcagga gtcggctttg ccggtattgc
     2881 cggcttcggc ggcggtttcg gccggagaat gctgaatgcg tggggcgaaa cgctcattaa
     2941 gcaattcgtg caagaatcca tcagtcacgc cgttcgcttg gaacaggcgc ttgccaagct
     3001 acaaaccgaa cgccgtattg ccgtgctgca ttattctccg attcgcgcga cgctggaagg
     3061 agaagacccg gaaatttttg cctttctcgg ttccagccga ttagaagagc caatcaatcg
     3121 atttcgcgtc agcgcggttt ttcacggcca tgcccacaac ggcattctcg atgggaaaac
     3181 ttcaacaggc attcccgttt ataacgtctc tgcgccggcg cttcggaaat ccggaaagcc
     3241 ataccggatc gtcgagctgt aggcccggcc ctaaccatcg tactttcggg tagcgcacgc
     3301 gtctcgcgtg ctggtttcct cgtctcgcca aaacagtctt tcccaatgct cacaatgatt
     3361 gggaaagcgt acccacaggc aaagtccgtg atcgcgagga cgcgatcacc agcacgcgag
     3421 aggcccgttc ggctgcgctt agggcaggca gtgcctaccc ggagagcgct ttcgttgcga
     3481 caacgcgcca cgcttcaaca tcggcgcggt ttcgcgtggg cgaggcgcaa cggatcgctt
     3541 ttattgactc aaaagtaact tccgaaaacg ggagcttaat tgagtgggcc agtcggcgtc
     3601 gcgccaagat cgacatcttc ttgtagagca agctcaagtg tggtttgaga tcgtaatcgg
     3661 acgaatcttg cgcagcatcg cgaatcattt cgttgagttg ctgcaatttt ctgtccagcg
     3721 cgaattgcac gaagagtgtt ttgataaact cgccggactg gcagatctta agcgccttcg
     3781 ctttgacggg tcggcaaccg ctaacagttt tcgtaattac ttcttcggcg tccactcgat
     3841 ctaagccgac atagatcgtc atgtgcggct caaagactgg cgcgctgtat cgtcgcgcaa
     3901 gatcaacaat cgtcctctca aggaaggagc gcgccgggtc agccggaata agccagtagg
     3961 ttatcgcagt ttttttcatg cttgaagaac acactcgttt ctcacgcgca ttttcaacaa
     4021 tccaatattt aacaaattga acctccatgt taagatcgct ttgtgcccac caatgttctc
     4081 ggaggcgaac tgaaatgttg ctgtcgcaat ccggtcaccg gcttctatcg cgacggcttt
     4141 tgtcgcaccg gcccggaaga tgtcggtctc cacacggttt gtgtaaaggc cacgcaggaa
     4201 ttcctcgatt tttccgtgct cgacggaaac gatctggtta cgccgcgacc agaatgggga
     4261 tttcccggac tgaagcccgg cgacaaatgg tgcgtctgcg tcacgcggtg gaaagcggcg
     4321 ctcgaccaca atcgcgcagc tccggtcgat cttgaggcga ctcattcctc cgcgctcgaa
     4381 ttcgtcaccc tggaagaact aaaagcccac acggtgaaat agcggggagc acaggctgcc
     4441 agcctgcgcg cacagttgaa ctagagcggg tttgccgcca cgctaacccc gaattgaaac
     4501 tgccgtttag tctttttctg gcgctgcgat atttgaaacc gaagcgcacg tttctctcca
     4561 tcatcacatt gatttcggtg ctcggcgtga tgctcggggt gacggtgctg atcctcgtga
     4621 tttcggtcat gaccgggttc gatcgcgagc tgcgccagaa agtgatcgag ttcgatgcgc
     4681 atattctcgt gactaccgaa gacgtgttgc gcgactggcg aaccctcaaa gcgaaaatcg
     4741 acaagacgcc gggcgtcgtc gcgacggctc cctatatcca gggaccggtg atcgtggaat
     4801 ttcaaaaccg gcggctcgcg ccgctgatgc gcggtgtcga tcctgcggaa gaggagaaag
     4861 tgattccgct gaaaaaattc atcaagttcg gcaagcttga tctggaaggc gattcgaccg
     4921 tacttggggt tgagctcgcc cgaaaattgc aggttaacgt cggcgacaag ctgacagtct
     4981 attcgccggg gaatctcggg cagattctcg attccattaa aaagctggag aacgcgagag
     5041 gagaggacga aaaaaaagcg atcgatcaat tgcgcgacgt aatcctgcca aaggagctga
     5101 cagtcaccgg catttttgaa accggccatt acctgcacga ctctgaattt cttcttgtgc
     5161 cggtttatgt gggtcaggag ttatacggct tgggggacgc gttgcatggc atcacggtgg
     5221 caacggacaa cccgtacagc gcagaacgga taaaacaggc gatccaacaa tttcttgagc
     5281 cgcccgaata cgcccagacc tggatcgaca tgaatcgtca gttttttgaa gccgtgcggc
     5341 tcgagcggac ggtgatgttt ttccttttgt ttttcatcgt ggtcgtcgcg gcattcggaa
     5401 tcatgagcac gcttatcacg gtgacagtgc aaaagcggcg cgagatcgga attatgaaag
     5461 cgctcggagc caaccttgct caaatcatct gggtttttct cggccaggga acagttgtcg
     5521 gcctcttcgg aactcttacg gggctcgggc tcgggatgac gttgatccgc taccgcaacg
     5581 aattcagccg ttggctcgct tcgacgttgc acatcgagat tttcccgcgc gaggtctatc
     5641 aattttcggc gatcccggcg gaagtcattc cgaaggacgt tgccatcatc tgcatcagcg
     5701 catttttcat ctgctcattt gctgcgctga ttcctgccta cttcgccgcg cgcctcgatc
     5761 cggtcaaagc gctgcgctac gagtaatttc atctcttact cctgctcgcc tgccgcgccg
     5821 tagcttggcg aaggcgggtg ctctcgctcg caattgaatt cacaaaagcc tcgagcgcac
     5881 gcagagcata atcatatccc cctggccatt cgctttggcg cgggccggca acgttaagga
     5941 tgtcgatctt gtgcttgcgg ataaaattgg caatcgactt cccagcgtct tcggcagaaa
     6001 tttttgatgc gtcgatcaag tgatggggcc gtttctgctc aacagaaaag cggacagttt
     6061 gttcagtccc accgctcaat tttccggagt aaataatgac ggttcccgca gaatctttta
     6121 cgttctgtaa agtccgctcg gtaaatccac cgtgtttcag ctccttcacg ggatagcgat
     6181 ctggaattct gccgaattca tcgaggtggc cgcctgggca ccacccgccg cattcgattc
     6241 cgtgtttcag cgccacatcg agcgcggcgc gatctacacc ggtttgtccg ccggaaatta
     6301 ttttcaccgg cctcaatacc ttcaatcgcc agtcggaaca atgtccgccc gctcgaccat
     6361 caaaatcacc gttcgttcag gtgcgcgtgt gcgatggcgc acgcctttcg gcacgacgaa
     6421 gccttgcctc ggtgcaagat caacaacgcg gtcttcgaga tcgatcagga aatgtccatc
     6481 gagaacaaaa aagaattcat cgatgtcttt gtgttcgtgc cagtgatatt cgccctgaat
     6541 caccgccatt ctcacgacag aatcgttcac cttgcaaagg gtctggttgt accatttgtc
     6601 ggtgcaagca tcggccagtt tctggacatc gattagttca agcggtccgt gcagaatgtt
     6661 gaggtaagtc ttgtacggaa aatcctgccg cgacttcttg gaatccatgg ggtgagttta
     6721 atcgcagatc aggccgtggt cgcaaaataa ctgtagccgc aagcgtgtcg cctgcgagtg
     6781 cgattccgac aatagatcgc taaacggatg ggattattct tcgctgtcga aataatcggt
     6841 ttcttcgccc ttcacgacga cgcgatccgc agcggatgac ttatttactt tccacttgcc
     6901 actgtctggg tagtagcacg attcgccgat cgggttatcg gcgatcacgt aatagataaa
     6961 gtcttcctga ccggggttac tgagttgatg gggctcgtcg ggcccgaaaa tgaaggcgtc
     7021 gccgggcacg acttcagttg taccatcttt gtgacggacg ctgcctttcc cggaaatgac
     7081 caaataaagt tcccattgcg ctgaatgcgc gtgataggga aaagctcgtt tcccaggagg
     7141 cactcgattc cattccagat caaacggatg gcgcttcgcc agatcgagcg agctcgggtc
     7201 gcgcccgagc gcctgtgaga ttcccttgaa gcaggcggca tatttcccgc ccggcgattg
     7261 ccacgtttct tctttgattt cgttcagatt tactttgcgc atttttgtca tgtcgagcgc
     7321 agtcgagaca tctctaactg tcatgtggtg tcttgacccg gctcgctatt cttcaccatc
     7381 gaaataatcg gtctccttcc ctttcacgat cataccttcc aatccctcct tgacgaccgc
     7441 ccatttgccg ctgtcaggat agtaacaaga gtcgccggtc gcgtcgccgc tgcgcggatt
     7501 atcggcgatc acgtaataaa caaattcttc atcaccggcg ttggcgatct ggtgcgcttc
     7561 gcccggctga aagaaaaacg cttcgccggc gattacagtg gtcgatccgc ttttatcgcg
     7621 cacgctcccg cgtccggaaa cgacgagata aagctccgac tcggccgagt gcgaatgata
     7681 cggacaatat attttgcctt tcggaattcg aaccatcgcg agatcaaacg gatgccgttt
     7741 cgccaaatcg agagactgcg gctcgcgtcc aagcgcaact gagatgtcct taacgaactt
     7801 ataaaacttg cccttcggcg attttcgctc ctgctcgggg atttccttga gattcacttt
     7861 gcgcatagaa aacaatttcc aatccgtttc gtaaaccgtc taacgagaag aacatcgcct
     7921 tcgcgttgcg atcgagaaaa cagcgatccc tatccaaatc atgttcacaa aagaagaagg
     7981 ataagctcga taaaagatcg tatttacggc caggagaagg ctgccgatga tattaagcga
     8041 ctggtaagca atcgagtcgc cttcgaattt cttggaggaa accatcgcat aagcaacaag
     8101 cagagcggct gctcccgccc atccgatcac atcaaaccac aaatgattgt tcatgctcta
     8161 taatctttgt cagaacaatg agatcatatc ttccacttct tcggtagccc ttgcttaagc
     8221 ttgtcgactg gctcgaacaa gtcccccagt ttttcaacgc gggcgagtgc ctggtcggag
     8281 cgacatttca aaaggtcggt ctttttcttt ttcaggcagt tcgcgacttc gtcccacgtg
     8341 actggagtgg aaacagtcgg ctcttccctg gcgcggagtg aataagcgca gatggttgtt
     8401 ttgtgttcgt cgttctggct ccaatcgacg aatacttttc ctttccgcac cgctttcgac
     8461 atgttcgagg tgacaagatt cgcatgttcg tgctcgagaa gctgggcgag cgcgcgcgaa
     8521 aggtccttcg tctgatcgta agtcacggcc gtattcagcg gcacgtaaac ttggaggcct
     8581 tttgagccgc tcgtcttcgc gaacgacttc agtttcattt tgcctaacgc ttcgcgaagc
     8641 caaagcccga cctggcagca ttggatgatg tcggcgggca ggcccggatc gagatcgaac
     8701 accatcatgg taggacgttc gatgttgttc ttgcgcgaga gcgacgtgtg cagctcgaga
     8761 tccgcgaggt tcgcagccca caccagtgtc ggcaaatcct gcgccaggca ataattcatg
     8821 atgcggttgt tcccttcgct ccaaacttgc gcggtcttta cccatttcgg ccgatgcgac
     8881 ggacaattct tctcatagaa aaactcacct tccacgccat ccggatagcg cttcatggtg
     8941 agcggccgat ccttgagatg cggcaaaaga accggcgcga tgcggatgta ataatcgatt
     9001 acgtgccctt tcgtaaatcc caccttcgga taaagcacct tgtccaggtt tgagaccgca
     9061 agcttgcggt cctcgacgat tagctcagct ttattcggca tgacttggaa gaaaacgtcc
     9121 aacatcgaac gcccaacgtc caacatcgaa ttcagagaag actcaggcga agaagcgctc
     9181 cggagatagc atgggctgcg gtttgcagat tcgggcgacg caatgctccc agctttcgtg
     9241 accgctgagg atttcgattt caacgcgcaa aaagcaaatc ggcaccttca cctccgcctc
     9301 gggcagatgc ggcatgcgga cagcgtcttt ttccgtcgta acaatcattg ccagatcgcg
     9361 gcgaatgcaa cgattgatga aggtcgttag ctccgcttcg gtatagtagt gatgatcgat
     9421 gtagcgtttt gccagatcga cgtgtgcgcc aagttttttt aatgcgcctt cgaaactttc
     9481 gggcgcggcg atgccgcaaa tcgagccgat gaacgcgtca tgcaagcgct cgagcggcaa
     9541 ccgttcgccc gtgaaaagat tcttaagata gagcggttta tgcgcgcact cgataatctc
     9601 ggcggtgcga ttgtaacgcc ggatgcgctt cacaagcgcg tcattaggct cccctgtggt
     9661 tttagtgatg aagatgtagc tcgcgcgccg cagattccgc ggcgcttccc gcagcgtgcc
     9721 gcggggaagc aggaattcat tgccaaacgg cgcctgccga tccaccagca cgatgtccag
     9781 gcgatgtttg agatggagat attgcaagcc gtcatcgagc agaagcgtat ctattttcca
     9841 tttgtcgatg gcaaagagac cactcttaac ccgatcttta tctacgagca cgaccacgtc
     9901 cttcaaattg tgcgctaaca tgtaaggctc gtcgccggcg gtgcgtgaat cgagcagcag
     9961 agattttccg tcagacacaa tccttggcgg atcgagcatg cttccgcgca gtcgatccaa
    10021 ccaacttcgt ttgcgcggaa cgctcttata accgcggctc aggatggcaa cgcgtcggcc
    10081 acccacctgc agggcacgtg caaacttttc gaccaccggc gtcttgcccg tgccgccaac
    10141 agtaaggttg ccaacgctga taacaagaca accgagcgcg cgctcacgga gaattcgttt
    10201 cctgtaaaag tagagacgaa gctgaacgag acgttcgtaa acgaacgaaa gcgcatggag
    10261 aattccgcgc aaaatggaag cgcgaatgcc atgacgacgc tcgagaacga cgtcgatgcc
    10321 gaattgttct aaattttcaa gccggcggcc catttcaatt gcggattgtg gattgcggat
    10381 tgcggattcg aaaaacacgc gcgccgtggt tgtctgcgcg cgtgatgatt atgcgcgccg
    10441 gtgtggagtt tgccgagcgt tccatggcag tcccaatttt cttgggcgaa cgaagggtca
    10501 cggcgcagat tgtcgatccg ctgccgctga ggaaagcgcc aagcgcgcca gctttctccg
    10561 cggcggcgat cacgcgcggg aggaacggaa tcagtttttt ccggtggggc tgatgcaggt
    10621 ggtcggcgaa agcgccgcgc aaatcacgat aattacctga cgcaaaggcg gctgtaattg
    10681 cacatgcatt tgcacaattt tccacggcag ccacacgctt tatttgcgac ggcaagatcc
    10741 gtctcgcccc cgatgtagtg attttgaaat ccggaatcaa gagcacgaaa gataaccgcg
    10801 ctgaaacgtc gaagcgctgg atggtctgac cccgcacaac agtgaaccca ccgaaggttg
    10861 ccggcgcggc gttatcggga tggccttcca actggacgca caattgaaaa atcgacaagc
    10921 gatcaacgcg attgccggtc aaagcgttca aagctcgcaa gacgccgagg cgcactgtga
    10981 cgctgctccc taacccgcgt gaaggaggaa tatggcccgt gatcgatacg gaaaatgaga
    11041 atggcgcgcg ccgggtatga cgaaaaaaca aatctgccgc ctcctctaca agag
//
LOCUS       DASQUQ010000007         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_902744, whole genome shotgun sequence.
ACCESSION   DASQUQ010000007 DASQUQ010000000
VERSION     DASQUQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902744"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VE486_00205"
     CDS             complement(<1..334)
                     /locus_tag="VE486_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZA37537.1"
                     /translation="MAWIGHATVLINFFGIKILTDPVLFPRIGIRLPGFTIGPKRLIA
                     PALEFYELPKIDILLLSHAHFDHLDLRTLHRFDPNTSVITASNTQDLLRWTRLRDITE
                     LHWHERKTL"
     gene            complement(601..1485)
                     /locus_tag="VE486_00210"
     CDS             complement(601..1485)
                     /locus_tag="VE486_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZA37538.1"
                     /translation="MKILICSDGTAAAESAIDLGGLLAGPLKAETTLLGIAEKSDDER
                     PLGEALQKQAQPLRAQHAVLEISVRAGEPIREILDQTSKTSYDLVVVGARWTGATGDH
                     WRSEKTYEVIKAIRPPVLVAIGECKRLKRFLVCTGGKEFIERAIEFTGRLAAAVGASV
                     TLLHVMAEPPAIYADLVQLEEDVDRLLESKSELGTNLRRQKRELERLGVSADVRVRHG
                     IVIDQVFEETREGDYDLIVTGTSQARGFLRHYIMGDLTRSILNRASCPVLVARAGKPA
                     AARTLWKALKEVFSTDEK"
     gene            complement(1492..2418)
                     /locus_tag="VE486_00215"
     CDS             complement(1492..2418)
                     /locus_tag="VE486_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZA37539.1"
                     /translation="MFFPISGADISPIYLVAVGFLVGILGGFFGVGGSFIAGPALRLA
                     GLDWNFAVGTDLAHIVGKSVVAAKRHRALGNVDLKLGLIMAVGTIGGAEVGAQAIQML
                     KRAGNVNLVVSVVSIVVYLSISGFMVWESRKTLKSPKGRLPQGMVKGAKTKMDHSAFG
                     PVTRSIQRLKIWPMISLPTSGIKEISLWMVILVSFIGGLFSGFLGGGAGYIRMPSMVY
                     VLGIPTHLAVGTDLFEIIISASYGTFSHGIKGNVDILIALVMHTGAAIGAQIGAISTQ
                     YFAGPKIRLAFVPLPLIGAAIVIYTLLSGHKL"
     gene            complement(2422..2664)
                     /locus_tag="VE486_00220"
     CDS             complement(2422..2664)
                     /locus_tag="VE486_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37540.1"
                     /translation="MKSETTKTLGAFAVELVIYGILVTGYFFLVLHLLGQWLYELEIH
                     HRYTYAILAILLIIGQAVLLESLTTFLIRLIRGRSE"
BASE COUNT          705 a          829 c          767 g          662 t
ORIGIN      
        1 tgagagtctt tcgttcgtgc caatgaagct ccgtgatgtc gcgcaaacgc gtccatctta
       61 acaggtcttg cgtgttcgaa gcagttatta cgctcgtgtt cgggtcgaag cgatgaagcg
      121 ttcgcaaatc gagatgatca aagtgagcgt gcgaaagaag gagaatgtcg atctttggca
      181 attcatagaa ttcgagcgcg ggtgcgatca aacgttttgg accaatggtg aatcccggca
      241 atcgaatccc aatgcgcgga aaaagtacgg gatcggtgag aatctttatg ccgaaaaaat
      301 taatgagcac agttgcatga ccgatccaag ccacggtcag gcgtgcacca ttccacttcg
      361 aaggctccgg cttggcaaaa gtcggggcga tgggccgcca actttcggtc gtccattctc
      421 gccaaagatg aacaagccaa cgacggacac gggagttgcg cagaactggt tgttcagacg
      481 gttccactaa tgaagttctt cgtcatttga gcattcgaac ttctttcgtc attcgtcatt
      541 cgtgcttcgt ccttttattg gttttcaatt ggcctcgaag acaccggcac gataggcccg
      601 ctacttttcg tccgtactga atacttcttt gagagccttc caaagcgttc tggctgccgc
      661 cggtttgccg gcgcgcgcaa ccagcaccgg acagctggcg cgatttaaga tgctgcgagt
      721 gaggtcgccc ataatgtaat gacgaaggaa accacgcgcc tgcgacgtgc cggtcacgat
      781 caaatcgtaa tcgccttcgc gtgtctcctc gaaaacctga tcgatcacta tgccgtgccg
      841 aacacgcaca tcggcggaga cgccaaggcg ttcgagctct ctcttttgcc ggcgcagatt
      901 tgttccgagt tccgatttcg attcgagcag gcggtcaacg tcctcttcga gctgcacgag
      961 gtctgcataa attgcagggg gctcggccat gacatgcagc aacgtcacgg aggcccccac
     1021 tgcggctgct agtctgcctg tgaattctat cgctcgctcg atgaattcct ttccgcccgt
     1081 gcaaacaaga aaacgcttca agcgtttgca ctcgccgatt gccacaagca ccggaggtcg
     1141 aatcgcttta atcacttcat aggtcttttc tgaacgccag tgatccccgg tcgcgcccgt
     1201 ccatcgcgct ccaacgacga cgagatcgta gctcgtttta gatgtctgat cgagaatctc
     1261 ccgaattggt tcgccagcgc gcacgctgat ttccagcacc gcatgctggg ctcgaagggg
     1321 ttgcgcctgc ttctggagcg cttcccccag ggggcgttcg tcgtcggatt tttcggcaat
     1381 gcccaggagg gtcgtttcag ccttcaatgg ccctgccaat aatcctccca aatcgatcgc
     1441 actttcggcg gctgccgttc cgtcactgca aatcaaaatt ttcatcgatg gttacaattt
     1501 gtgtccgctg aggagagtgt aaatcacgat cgctgcgccg ataagcggga gcggcacaaa
     1561 cgcgagccga attttcgggc cggcaaaata ttgggtggag atcgcgccga tctgcgcgcc
     1621 gatagccgcg ccggtgtgca tcaccagcgc aatcaggatg tccacattac cctttatccc
     1681 gtgactgaaa gttccatagc tggcggagat gatgatctcg aacaaatcgg tgcccaccgc
     1741 cagatgagtg ggaatcccga gcacgtaaac catcgagggc atgcggatgt aaccggcgcc
     1801 gccgccgaga aaaccactga agaggccgcc tataaacgag acgagaatca ccatccagag
     1861 cgaaatctct tttattcccg aagttggcaa cgagatcatc ggccaaattt tcaatcgttg
     1921 aatggaacgc gtgactggcc cgaatgcaga atgatccatt tttgtcttcg cccccttcac
     1981 cattccctgc ggtaatcgtc ccttcggcga ctttaatgtc tttcggcttt cccagaccat
     2041 gaatcccgag atgctcaaat aaacgacgat ggagacgaca ctcaccacca gattgacgtt
     2101 tcctgcgcgc ttcagcattt ggatcgcctg cgctcccacc tccgcgccgc cgattgttcc
     2161 cacggccatg atcagcccca gcttcaaatc gacgtttcca agcgcgcgat gccgttttgc
     2221 cgccaccacc gactttccaa cgatgtgggc aagatcagtg ccgaccgcga aattccagtc
     2281 caaaccggcc agccgcaagg cgggaccggc aatgaaactc ccgcctaccc caaagaagcc
     2341 gcccagaatt ccgacgagaa aacctacggc gaccaggtag atcgggctga tgtccgcccc
     2401 ggaaattgga aaaaacatgg cttattcgga gcggccgcgt atgagacgaa ttaagaaggt
     2461 ggtgagactt tcaagcaaaa cagcctgacc gatgatcagg agaatcgcga ggatggcgta
     2521 ggtatatcga tggtgaattt cgagctcgta cagccattgt cccagcaagt gcagtacgag
     2581 aaagaagtag cccgtgacca gaatcccata gatcactaat tcgactgcaa aggctccaag
     2641 agttttggtg gtttccgatt tcatcacgtg aatcgttaga tagttacatc gttaatcgtt
     2701 agaggagata caatggcctt gtcgattcaa acgatttaag cgccgcggaa gtctggcggt
     2761 tgcgtgcaag ctcgtccacg ggcaaattcc attgcgctga gagcgtttcc tcctaacgtt
     2821 caacttcact tttaccggaa aaactatggc agacgccgca aacaaatacg ccgaaaacgt
     2881 accgggcaaa ttctacgtcg atgaccaatg catcgactgc gatttgtgcc gcgagaccgc
     2941 gcctgccaat ttcaaacgca acg
//
LOCUS       DASQUQ010000008         9246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_541791, whole genome shotgun sequence.
ACCESSION   DASQUQ010000008 DASQUQ010000000
VERSION     DASQUQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541791"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..627
                     /locus_tag="VE486_00225"
     CDS             130..627
                     /locus_tag="VE486_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37541.1"
                     /translation="MDEFDDNEIARLLRLKRYEQPPPGYFENFLHEFHRRQRDELLRQ
                     PLWRICWERAHDFMFRLDVRSLASYPAAVAAVLVCAAVISLKIYQEPQTANVALQSPP
                     ALSEPANAEGEWTLASPVATQTFRTQPLRSFDQRAQTHSRSRGIPPRYVLDSVPVSYE
                     SNFRF"
     gene            605..1693
                     /locus_tag="VE486_00230"
     CDS             605..1693
                     /locus_tag="VE486_00230"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HZA37542.1"
                     /translation="MSRISDFSRAPQLPSRAQFASVCRLGLALGLSFAAVPLVYAQEQ
                     SAITISQQVKDIFGRTANAVVKIHGADEHSEISGTGFFIDPTGTLYTAYTVGGEADNF
                     TIEFDGKKYPARQLLADIRSGTAILKIDAVTPALPIGKSEELQVATPVVAIGYPLDLP
                     KTPNFGMVAGFDRKYLGRYFSTTHLRLNLPTQRGEAGAPLLNMNGEVVGIVVSSLENN
                     SSCYAVPIEAAEKIRSDFVRFGEARHGWVGINVSEAPAPVEGSRAEMTQIMDGTPAAS
                     SGVKPGDILLQVGRTKITQPEDVLNASFFITAGDTVPITVMRGDEKLTFNVRAELHPA
                     SERGTLIASPTINRAIPLKLDSEGQRTP"
     gene            complement(2337..2567)
                     /locus_tag="VE486_00235"
     CDS             complement(2337..2567)
                     /locus_tag="VE486_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZA37543.1"
                     /translation="MKLPKLTAVIEPGEDGWYVANCPELGIVSQGETPEHAEEMIKEA
                     VELWLECAHESEVRRRLEERKVAIKELQLTHA"
     gene            complement(2901..5057)
                     /gene="ligA"
                     /locus_tag="VE486_00240"
     CDS             complement(2901..5057)
                     /gene="ligA"
                     /locus_tag="VE486_00240"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZA37544.1"
                     /translation="MAMEEKQAAKRVAHLREQIREHDRRYYEEAAPIISDREYDRLYK
                     ELVDLETKFPDLITPDSPTQRVGGKPLKAFEQVSHLIPMLSLDNTYSEEEVKNFYARI
                     RRLLPNEKIPVVIEPKVDGVAVSLIYENGKLRQAATRGDGSVGDNITQNIRTIRSVPE
                     RLRGAAPKVLEVRGEVYMDRKGFEKLNQERKEQGLPLFANPRNAAAGSLKQLDPAIVA
                     KRPLGLVLYGTGATEGVDVDVHSEVFPLLKKLGLPVTERWWLAKSVEEILDAIHELDG
                     IRQKFAYQTDGAVVKVNSFAQRERLGFTAKSPRWAIAYKYEAERVETRLLDIVIQVGR
                     TGILTPVAVLEPVLVSGSTVGRATLHNEDEIKRKDIRIGDTVVIEKAGEVIPAVVEVM
                     KSKRPRDAAPFDFFKHIHGKCPACGSPVRRDPDFVAWRCENLQCPAQTTRRVEFLAAR
                     GALDIESVGGIVADKLVERGLVREPLDLFELKIDQVATLNLGTEEAPRIFGEKNATKA
                     IQAIERARTLPLSRWLFALAIPEVGKTTATQLARFHDTIEDVANSPLLRDVLEYHEKR
                     DQRASAKEIAERLIKTGFAERSKSKVGKDGIVTEVGPVVAQSVLDFFASTAGKKILHR
                     LKRLGIQPKSEKISPKKAEELPLTGKTFVLTGALPSMTREEATEKIEALGGRVTSSVS
                     KKTDYVLAGAEPGSKFDKAKELGVKIIDETAFRKML"
     gene            5341..7116
                     /locus_tag="VE486_00245"
     CDS             5341..7116
                     /locus_tag="VE486_00245"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HZA37545.1"
                     /translation="MKPGERILGVEGGGTKTAWVLVERDGDEMRILDQGKLPPSNFRL
                     VTLDRLRGILSALPKEIDRAGVFLAGCGPEDRSILSPLCGEIWPTAKIITGNDRDSGL
                     AAALGSGEGIVVNAGSGSSVTGRRGDRIEKAGGWGHILGDAGGGYFLSIQALRLILRE
                     YDLHRGEMQFAAKVLQALSLNNLDELVRWAQTADKMEIAMLTPVVFQAAASGDMRVME
                     ILESGAQVLCEYTEAVASRLHLLAPKVVLMGGLFHRDSIYTHAFRRRLKKNLPDVRVA
                     TAERSPELGAAWLAAELGDHVTFQPRLSQNEIENLAVALTEQRNPRSENLERMKAREL
                     VELFIEEEKFVQDALRGATVELTRATEIVAESLRNGGRMFYVGAGSSGRIGVLDASEI
                     PPTFGASSDLVQGVIAGGATALYRSVEGAEDEQSTGALAMSERGINNKDVVIGITASG
                     RTPFVLGATGRAKSLGAKTILLTCNPAGVAGVSPAAAGTAASTTNVDLAIHLAVGPEL
                     LTGSTRLKAGTATKVALNIISTGAMVALGKVRGNLMIDLRAASTKLRDRAVRVVAGLA
                     KCDYDSARKVLEESDWNLRAVVERL"
     gene            7267..7416
                     /locus_tag="VE486_00250"
     CDS             7267..7416
                     /locus_tag="VE486_00250"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZA37546.1"
                     /translation="MLWTIFVILLVLWLLGLVSSYTLGGFIHLLLVIAIVILIINLIQ
                     GRRPL"
     gene            7427..7648
                     /locus_tag="VE486_00255"
     CDS             7427..7648
                     /locus_tag="VE486_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37547.1"
                     /translation="MKPVAIVGIILIVIGIIGFAYGGLGWTTEKKDAQLGPLEITHKE
                     RHGIAFPPIASGICLVGGVILVLVGSRKS"
     gene            complement(7656..>9246)
                     /locus_tag="VE486_00260"
     CDS             complement(7656..>9246)
                     /locus_tag="VE486_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZA37548.1"
                     /translation="TYVLSGRATAGRWNGEGAFEQTGIGRAQLNIACSMLSKFQPMRL
                     SILILSVAITSSFAAQPEVVPKPTVPAAAAEAGKDVVHQLNNAFAKVFEVVAPSVVII
                     KISKKNEVAERSPLDDLFFQGPLDENTPRRAPPGGIQPIQSEGSGFIVRPDGYIFTNF
                     HVVEGADKIEVRLRDGRDFPARVMGTDEKTDIAVIKIDAKDLPVVQMGDSDAVRVGQF
                     AFAIGAPFKLDYTFTYGVVSGKGRDKLLSSGGYSISDYLQTDASINPGNSGGPLCDID
                     GKVIGMNTLINGMNRGLGFAIPINMAKEIGAELIAGHKIVRPWLGIRIETLGEDPATR
                     DLFKGADKGVIVRTIEADAPASRSDLRPFDVITRVDDKAVASDTQLQHEILKKKVGQN
                     VDLTVWRKGKTIKVPVKTGELPNEIARASNESIQPSQPKEQDTGKFGLQVQELTKDVA
                     ERLHLAVQQGVIVTDVADNSLAAQQDIQREDIITEVDGKPVTNVQSFREALTKADPRR
                     GVLLYLDRKGSKTFAVLKAGG"
BASE COUNT         1908 a         2629 c         2569 g         2140 t
ORIGIN      
        1 cgcggagatt agtaaaatct tgcgtgtctc ggaaggaacg gtacgttcga ggctctttta
       61 cgcgaatcgg caattgcaaa attatctgga cgaatttcgg aaaaatcctg tttcttaacc
      121 ggcgctgaaa tggacgaatt tgacgacaac gaaatcgcga ggctgcttcg attgaagcgc
      181 tacgagcaac cgccgcctgg ttatttcgaa aatttccttc acgagttcca tcgccggcag
      241 cgcgatgaat tgctccgcca accactttgg cggatctgct gggaacgcgc tcacgatttt
      301 atgttccgat tggatgttcg ctccctggct tcgtatcccg cggctgtagc ggcagtcctg
      361 gtatgcgccg cagtcatttc attgaagatt tatcaggaac cacagaccgc caatgtcgct
      421 ctccagagtc cgccggcatt atccgaaccg gcgaatgctg aaggcgaatg gacgcttgcg
      481 tctccggtag ccacgcagac tttccggacg caaccgcttc gtagctttga tcagcgcgcc
      541 cagactcact cccgcagtcg cggaatcccg ccacgttacg tgctcgatag cgtccccgtg
      601 agttatgagt cgaatttcag attttagtcg cgcaccgcag cttccctccc gggctcagtt
      661 cgcgtcagtc tgcagactcg gcctcgcgtt gggtctttcc tttgccgcgg ttcctctcgt
      721 ttacgctcaa gagcaatccg ccattacgat tagccagcag gttaaagaca tctttggacg
      781 cacggccaac gcagtagtta agattcacgg cgccgacgag cacagcgaaa tttcgggcac
      841 cggatttttc atcgatccta ccggcaccct ttacactgca tacacagtcg ggggtgaagc
      901 ggacaatttc acgattgaat ttgatggtaa gaaatatcca gcgcgccagc ttttggcaga
      961 tatccgcagt gggacggcga tattgaaaat agacgcggtc actccggcgt tgccgattgg
     1021 aaaatccgag gagctgcaag tggccacgcc tgttgtcgcg attggttatc ccctggattt
     1081 gccgaaaaca ccaaatttcg gaatggtcgc gggattcgac cggaaatatc tgggccgcta
     1141 tttttcgacc actcacctgc gtctcaacct gccgacgcag cgcggagagg ccggcgcacc
     1201 gctcttaaac atgaacgggg aggtggttgg cattgtggtc agcagtctcg aaaacaactc
     1261 ttcgtgttac gccgtgccaa tcgaagcggc ggagaaaatc cgcagcgatt tcgtccgttt
     1321 cggcgaggcc cggcacggtt gggtggggat aaatgtttcc gaggcgcccg cgccggtgga
     1381 gggttcccga gcggaaatga cgcagatcat ggatggcaca ccggcggcca gctccggagt
     1441 caaacccggc gatattctgc tgcaagtggg acggacgaaa ataacgcaac cggaagatgt
     1501 gctgaatgct tcgttcttta ttactgccgg ggacacggtt cctatcaccg tgatgcgcgg
     1561 ggacgaaaaa ctcacattta acgtgcgggc tgaacttcat cctgccagcg agcgcggcac
     1621 actgatcgct tcccccacca taaatcgcgc cattccgctc aagctcgatt ccgagggaca
     1681 gcgaacgccg tagagtagcc ggagaatagg catttctgcc tgtgcggcat gcggacattc
     1741 ttgtccgtag tttattgaaa tcaccggcgc gcaactccgc tggctcgcga taagtttccg
     1801 gagctggcca cccaggctga aagaagccct tttgaaaacc ccaagccgcc tgtttgtcat
     1861 accgagcgaa gtcgaggaat ctctcattat cgaacagtaa tcccgccgcg gcgggagact
     1921 tcgctcgaca tgacagggcc gcgaagttgg attttctacg gagccactca aaggctctgt
     1981 tcggtaatct gtcgccccgc gcctatagca ttttccgaaa ctccgattcg tcgataatgc
     2041 gaacgccgag ttctttcgct ttgtcgaatt ttctgcctgg ttccgcgcct gccagcacgt
     2101 aatcggtctt catgcttagc gcttgagagg ggagctctta ctcgaagagc gaccggggaa
     2161 gtccgctttg acggatgatt ccacgcagcg tgccacgatc gagttcgcgg tgaagcggaa
     2221 ctggaaaaga gattcgaccg cggcgcatga tcacgtgact accacgttgc cgtacacgtt
     2281 caaagccctg tgtactcagc aaatcgcaca gctctgcgcc ggaaaacagg cgcagtttag
     2341 gcatgcgtca gctgaagctc tttgatggca actttgcgct cctccagcct gcggcgaacc
     2401 tcggattcat gggcgcactc aagccacagt tcgaccgctt ctttaatcat ttcttcggcg
     2461 tgttcgggcg tttctccctg actgacgatg ccgagttcgg ggcagttcgc cacataccat
     2521 ccatcttcgc cgggctcaat cacagcggta agcttcggaa gcttcatgac gagaagatta
     2581 ccttatctca ccatctttcg ctactaaacg taaatccaaa ttgctatagg ccaaattcgt
     2641 cgcgttttgc gaagattttt ttctccccgc gaatcacgcg gatcgacgcg aaacaaaaga
     2701 aggccggtta aaggcggaac tgcaaaaccc gaccacatcc atcgccccgt aacctcgaat
     2761 ttcgccggtt gtaaaaccgc cgttccttcc gctttttctg ttcgcgttaa ttcgcgtcat
     2821 tcgcgggctg aacttcggcc tgccacgccg aagcctggcg aaggcgggtt gcggggggat
     2881 ccgctctggg gaatcgtcgt tcacaacatc ttccggaatg cggtttcatc gataattttt
     2941 acgccgagct cttttgcttt atcgaatttg ctgcccggtt ccgccccggc caggacgtaa
     3001 tcagtcttct tactcacgct gctggtcacg cgtccgccga gcgcttcgat tttctcggtg
     3061 gcttcctccc gtgtcatcga agggagtgcc ccagtcagga caaaggtttt tccagtcagc
     3121 ggcagctctt ctgctttttt aggcgagatt ttttcgctct tcggttgaat ccctaatcgc
     3181 tttaggcggt gcaaaatttt cttgccagcc gtcgacgcaa agaagtcgag cacgctctgt
     3241 gcgaccactg gcccaacttc agtcacgatg ccgtcctttc ccactttgct cttcgatcgc
     3301 tctgcgaaac cagttttgat taatcgttcg gcgatttctt tggcggatgc ccgttgatcc
     3361 cgcttctcgt gatactccaa aacatcgcgg agcagtggtg aattggccac gtcctcgatg
     3421 gtatcgtgga aacgagcgag ctgcgttgca gtggttttgc caacctcggg aatggcgaga
     3481 gcgaaaagcc agcgcgaaag aggcaaggtt cgcgcgcgct cgatcgcctg aatcgctttg
     3541 gtcgcatttt tctcaccgaa aatgcgtggc gcttcttcag tgccgagatt caatgtagcg
     3601 acttgatcga tctttagttc aaacagatcg agcggctcac gcacgagccc gcgctcgacc
     3661 agtttgtccg ctacaatgcc gcccacactt tcgatgtcga gcgccccgcg cgccgcgaga
     3721 aattccacac gtcgcgtcgt ctgcgcgggg cactgaagat tttcgcagcg ccaggcaacg
     3781 aagtccggat cgcgccggac cgggctacca cacgccggac atttgccatg aatgtgcttg
     3841 aaaaaatcga aaggcgcagc gtcccgcggc cgtttcgatt tcatcacctc aacgacggcg
     3901 ggaatcactt cgcccgcctt ctcgatcaca accgtatcgc cgatgcggat gtctttgcgc
     3961 ttgatttcat cctcgttgtg caatgtcgcg cggccaacgg tgcttccgct gactagcacc
     4021 ggttcgagaa cggcgaccgg cgtcagaatt cccgtgcgcc cgacttgaat cacgatgtcg
     4081 agcaggcgtg tctcgacgcg ttcggcttcg tatttgtagg cgatcgccca ccgcggggat
     4141 ttagcggtga aaccgaggcg ctcgcgttgc gcaaacgaat tcactttcac taccgcgccg
     4201 tcggtttgat aagcgaactt ttggcggatg ccgtccagct cgtgaatcgc atccaaaatc
     4261 tcctcgaccg acttggccag ccaccaacgc tcggtgacgg gcaaccccag ctttttcagc
     4321 agcggaaaaa cttccgagtg aacatctacg tccacgcctt cggtcgcccc ggttccgtaa
     4381 agcaccaggc cgagcgggcg tttcgccaca atcgcgggat cgagttgctt cagcgaaccc
     4441 gccgcggcgt ttcgcggatt tgcaaacagc ggcaggcctt gctccttgcg ttcctgattg
     4501 agtttctcaa aacccttccg gtccatgtaa acttcgccgc gcacttccag aactttcggt
     4561 gcagcgccgc gcagtcgctc cggcaccgag cggatcgtcc ggatgttttg cgtgatattg
     4621 tcgccgacgc tcccgtcccc acgcgttgcc gcctggcgca gttttccgtt ttcgtaaatg
     4681 agcgaaaccg caacgccgtc cacctttggc tcgatcacca ccgggatttt ttcgttcggc
     4741 aaaagccgtc ggatacgggc ataaaaattt ttcacttcct cttccgagta ggtgttatcg
     4801 aggctgagca tcggtataag atgcgagacc tgctcgaagg ctttcagcgg ttttccgccg
     4861 acacgctgag tgggtgaatc cggcgtaatg agatcaggga actttgtctc gagatcgaca
     4921 agttctttgt agaggcgatc gtactcgcgg tcgctgatga tcggcgcggc ttcttcgtaa
     4981 tagcgccggt cgtgctcccg gatttgctcg cgcagatggg caacacgctt cgcagcttgc
     5041 ttctcctcca ttgccattct gcacgatata agcagcgtga cgctcggcaa tgccggcacc
     5101 gatgcatgcg ggggcgcgtt tcgattccgg ggagcacagg ccgctggcct ctagttcgcg
     5161 gcagcttgcc gcgaaccgat ttcgcaagcc cgagcgttga tacgttacca aatgtattcg
     5221 gcacagcggc cgaatgcgac aggccagcag cctgtgctcc ccagaaagag aacggccggt
     5281 tacttctgct tttcagcaag gcttttaaat tcgcgcagaa tcagctaagc atactggatc
     5341 gtgaaacctg gcgagcgcat cctaggcgtc gaagggggcg gaacaaaaac cgcctgggtt
     5401 ttagtggaac gcgatggcga tgagatgcgc attctcgacc aaggcaaatt gccaccgtca
     5461 aatttccgcc tcgtcacgct tgatcgcttg cgcggcattc tttcagcgct gccaaaagaa
     5521 attgatcggg cgggagtttt tctcgccggc tgtggaccgg aagatcggtc catactctcc
     5581 cctctttgcg gtgaaatctg gccaaccgcg aaaatcatca ccggaaacga tcgcgattct
     5641 ggcctcgccg cagcgcttgg aagcggcgag ggaatcgtcg tcaatgccgg gagcggctcg
     5701 tctgtcactg gacgccgcgg cgaccggatc gagaaggccg gcggttgggg acacattctt
     5761 ggagacgccg gcggcggtta ttttctctcc attcaggcgc tgcgcctgat cttacgcgaa
     5821 tacgatttgc atcgcggcga gatgcagttt gccgcaaagg tgctccaggc gctttccttg
     5881 aacaatcttg acgaactcgt tcggtgggcg cagacggcag acaaaatgga gatcgcaatg
     5941 ctcacaccgg tcgttttcca ggcggccgcc agcggtgaca tgcgcgtcat ggagatcctc
     6001 gaaagcggtg cgcaggtcct ctgcgaatac actgaagcgg tcgcctcccg tctgcatctg
     6061 cttgccccca aagtcgtgct gatgggcggt ctttttcatc gcgactcgat ctatacgcac
     6121 gcattccggc gcagattgaa gaagaatctt cccgatgttc gcgttgcaac cgcggagcgg
     6181 tcgcccgagc ttggcgcagc atggctcgcg gcggagcttg gcgatcacgt cacttttcaa
     6241 cccaggcttt ctcagaacga gattgaaaat ctggctgtcg cgcttaccga acagcgcaac
     6301 ccgcgctcgg agaatctgga aagaatgaag gcgcgagaac tggtcgagct ttttatcgag
     6361 gaggaaaaat ttgttcagga cgcattgcga ggagcgactg tcgaattgac gcgcgcaact
     6421 gaaattgtag ctgagtcgct gcgaaacggc ggccgcatgt tttatgtcgg agccggcagc
     6481 agcgggcgaa tcggcgttct cgatgcaagc gaaattccgc cgacctttgg cgcgtcgtcc
     6541 gaccttgtgc agggagtgat cgcgggcggt gcgactgcgc tttatcgcag tgtcgaaggg
     6601 gcagaggacg agcaaagcac aggcgcgctg gcaatgagcg agcggggaat caataacaag
     6661 gacgtcgtta ttggcatcac cgccagcggc cgaacgccct ttgttctcgg tgcgacgggg
     6721 cgggcaaaat cgctaggcgc gaagactatt ctgctcacgt gtaatcccgc tggggtggca
     6781 ggcgtgtcgc ctgctgcagc cggcacggct gcctctacaa caaatgtcga tcttgcaatt
     6841 catctcgctg ttgggcccga gcttttgacc ggatcgacaa ggttgaaagc ggggacggca
     6901 acgaaagttg cgcttaacat cattagcacc ggcgccatgg tcgcgctcgg gaaagtccgc
     6961 ggcaatctga tgattgatct gcgcgccgcg agcacaaaat tgcgtgaccg cgcggtgcga
     7021 gtggtcgccg ggctggcaaa atgcgattac gattccgccc gaaaggtgct tgaagaaagc
     7081 gactggaatt tgcgcgcagt ggtagagagg ctctagccac ggtgcctcag atgctgatcc
     7141 agatcggcca caatcggcgg ctgcaaacta tgccgcgaag aaaatctttg gagaatcttt
     7201 gatttcaata agcgcgacgc aagcatctcg cacgaagcaa ggtatgactt aaccgaaagg
     7261 aaccttatgc tctggacaat attcgttatc ttgcttgtgc tctggctgct tggactggtc
     7321 agctcctaca cattgggcgg attcattcac ctcctgctcg ttattgcgat tgtaattctg
     7381 attatcaatt tgattcaagg ccgccgtcca ttgtaacctc gcaccaatga aaccggtcgc
     7441 gatcgttggc attattctca ttgtcatcgg gatcatcggc tttgcttatg gaggcctggg
     7501 ttggacgact gagaagaaag atgcccagtt gggtcccctt gaaattactc acaaggaacg
     7561 tcacgggatc gcttttccgc cgatcgccag cggaatttgc ctcgtgggcg gagtgattct
     7621 tgtgctcgtc ggcagccgga aaagctaatc gactgctagc cgccagcttt cagcacggcg
     7681 aaagtcttgc tccctttgcg gtcgaggtag agcaggacgc cgcggcgcgg gtcggctttc
     7741 gtgagcgctt cgcggaatga ttgcacgttg gtgaccggtt tgccatccac ttcggtgatg
     7801 atgtcctcgc gttgaatgtc ctgttgcgca gccagactgt tgtccgctac gtctgtcaca
     7861 atgactcctt gctgaacagc gagatggagc cgttcggcaa cgtctttggt caattcctgt
     7921 acttgcaagc caaacttgcc cgtatcctgc tctttcggtt gagaaggttg gatcgattca
     7981 tttgacgccc gcgcgatctc gttcggcaat tcacccgtct tgaccggcac cttgatcgtc
     8041 tttcctttgc gccaaacagt caggtcaaca ttttgaccga ccttcttttt caagatctca
     8101 tgttgaagct gcgtgtcgga cgcgaccgct ttgtcatcga cccgcgtaat cacgtcgaac
     8161 ggacgcaggt cgcttctgga cgctggcgca tctgcctcga tcgtgcggac aatgacgcct
     8221 ttgtcggccc ctttgaatag atcacgggtc gcagggtctt cgccgagggt ttcgatgcgg
     8281 atgccaagcc acggccgcac gatcttgtga ccggcgatca gctccgcgcc gatctctttt
     8341 gccatattaa tcggaatggc gaaacccagg ccgcgattca tcccgttaat gagcgtgttc
     8401 atgccgatga cttttccgtc aatgtcgcac agcgggccgc cactgttgcc gggattgatc
     8461 gacgcgtctg tctgcaaata atctgagatg gaatagcccc cgctagaaag taatttgtcg
     8521 cggcctttgc cgctaaccac accgtaggtg aaggtgtaat cgagcttgaa cggcgcccca
     8581 atcgcgaacg cgaactgacc tacacgcacg gcgtcgctat cgcccatttg cacaaccggc
     8641 aaatctttcg cgtcgatttt aatgacggcg atatcggtct tttcatcagt gcccatgacc
     8701 cgggctggaa agtcgcggcc atctctcaat ctcacctcga ttttatcggc gccttccacg
     8761 acgtgaaagt tcgtgaagat ataaccatcc ggccgaacaa tgaagccgga gccctcgctt
     8821 tggatcggct ggattccgcc ggggggggct cgccgcggcg tattttcgtc gagtggccct
     8881 tgaaagaaca ggtcatcgag tggcgatctt tcggcgacct cgtttttctt tgaaatctta
     8941 ataatgacga cgctgggagc gacgacttcg aaaacctttg caaacgcatt gttcaactga
     9001 tggacgacgt ccttgccggc ctcagccgca gcggcgggca cggtgggctt agggacaacc
     9061 tcgggctgcg ctgcaaacga actagttatt gcaacgctca agattaaaat ggataaacgc
     9121 attggttgga acttggacag catggaacat gcaatgttca actgggcgcg tccaatcccg
     9181 gtttgctcaa aggcgccttc gccgttccat cgtcctgctg ttgctctgcc agacaggaca
     9241 tatgta
//
LOCUS       DASQUQ010000009         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1264308, whole genome shotgun sequence.
ACCESSION   DASQUQ010000009 DASQUQ010000000
VERSION     DASQUQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264308"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /gene="atpD"
                     /locus_tag="VE486_00265"
     CDS             complement(<1..1192)
                     /gene="atpD"
                     /locus_tag="VE486_00265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZA37549.1"
                     /translation="MNTGNIVQVIGPVVDVEFPQGNGLPKIYNALEIEYEVNGNPTKL
                     TLEVQQHLGENWVRSIAMSSTEGLKRGMSVTDTGGPISVPVGEGILGRLFNVTGDPVD
                     GRGPVKFEKRYPIHRKAPDLTEQDTKVQILETGIKVIDLICPFSKGGKVGAFGGAGVG
                     KTVIIMELINNIAKGHGGVSVFAGVGERSREGNDLYNEMSEAGVIDQKDLSRSKVGMV
                     FGQMNEPPGARLRVGLSALAMTEFFRDERNQDVLLFIDNIFRFSQAGSEVSALLGRTP
                     SAVGYQPTLAAEMGDLQERITSTNKGSITSFQAVYVPADDLTDPAPANTFAHLDSTIV
                     LERSIAELGIYPAVDPLASTSKSLAPEIVGEEHYNVARGVQRVLQRYKDLQDIIAILG
                     MDELS"
     gene            complement(1201..2076)
                     /gene="atpG"
                     /locus_tag="VE486_00270"
     CDS             complement(1201..2076)
                     /gene="atpG"
                     /locus_tag="VE486_00270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HZA37550.1"
                     /translation="MANTQDIRRRIKSIRNTAQITKAMQMVAASKMRKAQQQALSGRP
                     YAELMNKVLVSLQRRTDPRLHPLLEIRPLKRELVLIISTDKGLAGALNTNLFREAANF
                     DSAKAAFVVTGKKARQFIARTKRELLADFELKDAPSFVETKPISKFCTEKFLNREVDK
                     VSVLHTHFINTINQRPVVQTLLPISGFDLPKKQEGQDTSQSADPMIGYIFEPNAERVL
                     DVMLPYYLQYQVFQMILDARASEHSARMVAMKNATDNANQFIKDLTLEYNKMRQAGIT
                     TELLEIATAQMALSS"
     gene            complement(2184..>2694)
                     /locus_tag="VE486_00275"
     CDS             complement(2184..>2694)
                     /locus_tag="VE486_00275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012527.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZA37551.1"
                     /translation="NVISITDGQIYLETDLFYQGIRPAISVGLSVSRVGSAAQIKAIK
                     QVAGKVKLDLAQFRELAAFAQFGSDLDAATKARLDRGQRIVELFKQKQYNPIPVEEQV
                     AVMWAMQNGYVDPVPVERVKEFQLKLQDYLQTRKESLMASIREKKQLDAELESQLKSA
                     LDEFKATWR"
BASE COUNT          559 a          749 c          713 g          673 t
ORIGIN      
        1 ggcttaactc gtccatcccc agaatggcga tgatgtcctg gagatctttg taacgctgca
       61 aaacgcgctg cacgccgcgc gcgacgttgt agtgttcttc gcccacaatt tccggagcga
      121 gcgatttcga tgtcgatgcc aacggatcga ccgctggata aattccgagt tcggcgattg
      181 agcgctcgag gacgatcgtc gaatcgagat gcgcgaacgt gttcgccggc gcgggatcgg
      241 tgagatcgtc cgccggaaca tacactgcct ggaacgaagt aatcgaccct ttgttggtcg
      301 atgtaattcg ctcctgcaaa tcgcccatct cggcggccag cgttggctga taaccgaccg
      361 cggacggagt gcgacccaac aacgccgaga cttccgagcc tgcctgtgaa aagcgaaaaa
      421 tattgtcgat aaaaagaagc acgtcctggt tgcgctcgtc ccggaaaaat tctgtcatcg
      481 caagcgcgga aagtcccacg cgcaggcgcg cgccgggcgg ttcgttcatc tggccgaaga
      541 ccattccaac tttcgatctc gacaaatctt tttgatcgat cacgccggcc tcactcatct
      601 cgttgtaaag gtcgttgcct tcgcggctgc gttcccccac cccggcaaac accgagacgc
      661 cgccgtgacc tttagcgatg ttgttgatga gctccatgat aatgacggtc ttgccaacac
      721 cggcgccgcc aaacgcgccg accttgcctc ctttcgagaa cggacaaatc aaatcaatca
      781 ccttgattcc cgtctcaagg atttgcactt tcgtgtcctg ctcggtcaga tcgggtgctt
      841 tgcggtggat cggataacgc ttctcgaatt tcacgggacc gcggccatca accgggtcgc
      901 ccgtcacatt gaaaaggcgt ccgagaatcc cttcgcccac cggaacgctg attggcccgc
      961 cggtatcggt cacgctcatc ccgcgtttga gaccttccgt ggatgacatg gcgatcgaac
     1021 gcacccagtt ttcacccaga tgctgctgga cttcgagagt cagcttggtc ggattgccgt
     1081 tgacctcgta ctcgatttcg agcgcgttat aaatttttgg caaaccgttt ccctgcggga
     1141 attccacgtc cacaacggga ccgatcactt gaacgatgtt tcctgtattc ataaaactga
     1201 ttaagagctg agcgccattt gcgcggttgc gatctcgagc aactcggtgg taatgccggc
     1261 ctggcgcatc ttgttgtatt ccagcgtcag gtccttaatg aattggtttg cgttatcggt
     1321 ggcatttttc atcgccacca tgcgcgcgct gtgctcggaa gcgcgcgcgt cgaggatcat
     1381 ctggaacacc tgatactgaa ggtagtaagg cagcatcaca tcgagaaccc tctccgcgtt
     1441 cggttcgaaa atgtagccga tcatcggatc ggcgctctgg cttgtgtcct gtccctcctg
     1501 cttcttggga aggtcgaagc cgctaattgg cagcaacgtc tgaacaaccg ggcgctggtt
     1561 gatcgtgttg atgaaatgcg tgtgaagcac ggaaacttta tccacttcgc ggttgaggaa
     1621 cttttccgtg caaaatttcg atatcggttt ggtttcgaca aaacttggcg cgtctttcaa
     1681 ttcgaaatcg gccagtaact cgcgtttcgt gcgcgcgata aactgccgcg ccttctttcc
     1741 ggttaccacg aatgctgcct ttgccgaatc aaaattcgcg gcttcgcgaa agagattcgt
     1801 gttcaaggcg ccagccaatc ccttgtcggt gctgatgatc aggaccagct ccctttttag
     1861 aggcctgatt tcaagaagtg gatgcagccg gggatcggtg cgtctttgca gcgagaccag
     1921 gaccttattc atcaactctg catacggccg tccggacagc gcctgctgct gcgcctttcg
     1981 catcttggac gcagccacca tctgcatggc ctttgtaatc tgcgcggtgt tgcgaatcga
     2041 tttgatccgg cgccgaatgt cctgagtgtt tgccatctat cgaatttttg atttgcgatt
     2101 tgcgatttgc gatttgatcg cgctttcgcc attgtcgatc ttgcgaattg cagacacaac
     2161 tggatcaaat tcgtgcagcg agatcatcgc caggttgcct tgaattcatc gagcgcgctt
     2221 ttcaattgcg actccagttc cgcgtcgagt tgtttcttct cgcgaattga agccattaaa
     2281 ctttctttgc gggtctgcag gtaatcctga agcttcaatt gaaactcctt cacccgctcg
     2341 actgggacag gatcgacata tccattttgc atcgcccaca ttacggcgac ttgttcttca
     2401 accgggatcg ggttgtattg tttttgctta aaaagctcga caatgcgttg cccgcgatcg
     2461 agtcgcgcct tggttgcggc atccagatcg gaaccaaatt gcgcaaacgc ggcgagctcg
     2521 cgaaattgcg cgaggtcaag cttcactttg ccagcgacct gcttgatcgc tttgatctgg
     2581 gcggcggaac cgactcggct cacggaaagc ccaactgaaa tggccggccg aatgccttgg
     2641 tagaaaagat cggtctcgag ataaatctgt ccgtcggtga tcgagatgac attg
//
LOCUS       DASQUQ010000010         6563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_3592, whole genome shotgun sequence.
ACCESSION   DASQUQ010000010 DASQUQ010000000
VERSION     DASQUQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3592"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..1087)
                     /locus_tag="VE486_00280"
     CDS             complement(380..1087)
                     /locus_tag="VE486_00280"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37552.1"
                     /translation="MSIFAALNVYRDITHRSAAINMAIDEALLESATSPSIRLYRWAS
                     PALSFGYFGRFADVADNATERDLVRRWTGGGIVFHGEDFTYSIVIPASDPAFAESPMS
                     FYENVHRALSRTFVQMGREAVVNAGADHGLYNRPRAAASSARGYNGHCFANPVRADVM
                     IDGRKVAGAAQRRTRGGLLQQGSVQYVDLENGFAERFAQALSANCSEAKIDDAVLNRA
                     RQLAHQKYATDVWLRRR"
     gene            complement(1094..1786)
                     /gene="pyrF"
                     /locus_tag="VE486_00285"
     CDS             complement(1094..1786)
                     /gene="pyrF"
                     /locus_tag="VE486_00285"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZA37553.1"
                     /translation="MTTPADRIIVALDVATKKEALALVDQLRKQISFFKIGLQLYTTE
                     GPEIVRAVLSAGAKVFLDLKLHDIPNTVARAVESASNLGVHMLTIHLSGGSEMIRAAT
                     AARTNNLSILGVTVLTSAMEQTLREIGIADKVEDQVLRLAKLGVETGVEGIVASPHEI
                     TTIRREFGDKIKIVVPGIRPSGSESADQKRVMTPRQAIHAGADYLVIGRPIIAHSRPD
                     EAVTKILEEVGD"
     gene            1962..2792
                     /gene="ispE"
                     /locus_tag="VE486_00290"
     CDS             1962..2792
                     /gene="ispE"
                     /locus_tag="VE486_00290"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZA37554.1"
                     /translation="MQVFAPAKINLSLRILGRRSDSLHEIETVIASISLFDEIEIHKR
                     EDGIEFRCDDPSVPIGDDNLAVRAAKVFFAATKTKPAVSIELTKRIPYGAGLGGGSSD
                     AASTLLALNELFETKLPREALAKLAEPIGSDVPFFIFESAAICRGRGELVAPVRFPEK
                     LSILLLKPAFAVATGWAYSRWKDSIEIPGAPYAAQEFAGHTFVNDLERPVIEKFVFLA
                     QLKTWLLSQSEVGAALMSGSGSTVFAVMRENADVDLVAKRAKAALDPELWICACNTIG
                     "
     gene            complement(2960..3715)
                     /locus_tag="VE486_00295"
     CDS             complement(2960..3715)
                     /locus_tag="VE486_00295"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HZA37555.1"
                     /translation="MNFKLRIWRQPNPRAPGKLVDYEARDISPNTSFLEMLDILNEKL
                     LGRGDEPIAFDSDCREGICGTCSLTINGEAHGPDHPGAVCELYMRKFRDGATITVEPF
                     RVRSFPIIKDLVIDRSALDRIVQAGGFISARAGSAPEANSTPIPKHDSDLAMEAAACI
                     GCGACAAACPNGSAMLFTAAKVSHLSLLPQGDPERARRVLKMVRVMDAGGFGNCTNTY
                     ECEAVCPAEISASFIAKLNREYARAAIQRGAGE"
     gene            complement(3826..5754)
                     /locus_tag="VE486_00300"
     CDS             complement(3826..5754)
                     /locus_tag="VE486_00300"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HZA37556.1"
                     /translation="MIGTLDAKIPHGPLDQKWRSHRDHSRLVSPNNRRKFDIIVVGSG
                     LAGASAAATLGELGYRVKCFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDT
                     IKGGDFRSREANVYRLAEVSNLIIDQCVEQGVPFAREYGGLLANRSFGGAQVSRTFYA
                     RGQTGQQLLLGCYQALCRQIAAGTVQMFPYTEMLDLVIVDGHAKGIITRSLRSGRIER
                     HAGDAVVLATGGYGNVFYLSTNARGCNVTATYRAYKRGALFANPSFTQIHPTCIPVSG
                     EHQSKLTLMSESLRNDGRVWVPKRKEDREKAPSEISEQDRDYYLERKYPSYGNLAPRD
                     ISSRSAKEVCDEGRGVGPGGRGVYLDFADAIKRLGGDTIRERYGNLFEMYEKITGEDA
                     YKVPMRIYPAVHYTMGGLWVDYNLMSNVPGLHVIGEANFSDHGANRLGASALMQGLAD
                     GYFILPYTLPNYLAGQMGRRPSADSPEFQKTESEVRARIRRLLDVDGKRSLDSIHREL
                     GLLLWDKCGMARHAKGLHEALQTIPELREKFWREVRVPGDDEDFNQSLERAGRVADFL
                     EFAELMCADALARGESCGGHFREEHQTPEGEALRDDENHAAVFAWEFQGDPATAGPRL
                     HREPLHFETVHLTQRSYK"
     gene            complement(5751..6446)
                     /locus_tag="VE486_00305"
     CDS             complement(5751..6446)
                     /locus_tag="VE486_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HZA37557.1"
                     /translation="MNILVAFYRSSVGKKMIVAITGIILILFVLGHLLGNLQIFLGPD
                     WINGYSQHLRDLGPLLWLIRIFLLLTVIVHIYVTIQLAIENRSARPDRYIDRQYVKAT
                     FASRHMVMSGLIVLAFIIYHLAHFTVRVTDPRFGLLKADPLGRYDVYSMMVYGFQSYL
                     VSGFYLLGLFLLALHLSHGSSSFFQSLGLNDKKLTPRLAVGGRIFAWLLFAGYTSIPI
                     AILLGLVKPAQQL"
BASE COUNT         1426 a         1844 c         1849 g         1444 t
ORIGIN      
        1 cgcagacgtt tgccccggcc accaacagcg taggccacat aaccttcgat gtcgggatca
       61 aaagccgccg cctgttggac gatccttttt tcaacttctt ccagctgtgg ctggaccaac
      121 tgcgccaccc gagccagcga gtcggaggaa agagctggcg cagtcatgct ggtcgatgca
      181 aaagcttcca cgagccgaga atagtcgggc gacttacgtg ttgtcaaaac aacacacgcg
      241 cgatttttcg tagggcgttt gactcgctcg ctcccgctca ctaggtctct cgacctgccc
      301 gtctatgtcg ctcggctccc gccggctgca ttctctgaac ctcgaaagca ttcggggctg
      361 acacagccgc cctacaatct caccggcgcc gcaaccaaac atcggtcgca tatttctggt
      421 gagcgagttg acgcgcccga ttcaagaccg cgtcatcgat cttggcttca ctgcaatttg
      481 ccgataatgc ctgcgcgaac cgttccgcga aaccattctc gagatcgaca tattggacgc
      541 taccttgctg gagcaatccg ccgcgtgtgc gccgttgggc tgcgcctgca accttgcggc
      601 cgtcaatcat cacgtctgcc cgcaccggat tggcgaagca atgtccgttg tagccgcgag
      661 cgctgctcgc ggcggcacgg ggtctgttat ataaaccgtg gtcagcgccc gcgttgacaa
      721 ctgcttctcg ccccatctgg acaaaagtcc gactcaaagc gcgatgaaca ttttcgtaga
      781 acgacattgg cgactcggca aaggccgggt cgctcgcggg aattacgatt gagtaggtga
      841 aatcttcgcc gtgaaaaacg attccaccac ccgtccatcg acgcaccaga tcgcgttcag
      901 tagcattatc agcaacatcg gcaaatcttc cgaagtaccc aaaggaaagg gcaggggacg
      961 cccagcgata gagccgaatc gatggcgatg ttgccgactc gagcagcgct tcatcgatcg
     1021 ccatattaat agccgcggag cgatgagtga tgtcgcgata aacgttgagc gcagcgaaga
     1081 tcgacatctc gtgctaatca cctacttctt ccaggatttt ggtcacggct tcgtctgggc
     1141 gcgaatgcgc aatgatcggc cgcccaatta ccagataatc tgcgccggcg tggatcgcct
     1201 gacgaggagt catgactcgt ttctggtcgg cagattctga gccgctgggg cgaattcccg
     1261 gcacaacaat cttaattttg tcgccgaatt cccgacgaat cgtggtaatt tcgtgcgggc
     1321 tggcgacaat cccttcgact cccgtttcga cgcccaattt cgccaggcgc aagacctgat
     1381 cctcgacttt gtcggcaatt ccaatttcac ggagcgtctg ttccatcgcg cttgttaaaa
     1441 cagtcacgcc caggatcgat aggttgtttg tgcgagccgc agtcgcggct cggatcattt
     1501 cgctcccgcc gctcaaatga atggtgagca tgtggacacc aagattactc gcggattcga
     1561 ctgcccttgc gaccgtgttc ggaatatcgt gcagcttgag gtcgagaaaa actttagcgc
     1621 cggcggacaa gaccgcccgc acaatttcag ggccttcggt agtgtaaagt tggaggccga
     1681 ttttgaaaaa cgagatttgt ttccgcagtt gatcgacaag agcgagcgct tcctttttcg
     1741 tcgctacgtc aagcgcgacg atgattctgt cagcaggtgt tgtcattgca aacggtggaa
     1801 cacaggcctt tggcctcggt gatcagtggc catgttgtcc gctgtcttcg attcagcgga
     1861 ttgcaaaccc gctgggcgca cagactgaaa gtctatgttc cgctcccaat gcgccgattt
     1921 catcctgttc ttcttcggtt ttgccgaaca aattgcaagc tatgcaagtt ttcgcgcccg
     1981 cgaaaatcaa tctttcgctg agaattcttg gccggcgcag cgacagcctt catgaaattg
     2041 aaacagtgat cgcttcgatt tcgctttttg acgaaatcga gattcataag agggaagacg
     2101 gaatcgagtt tcgttgcgat gacccatcgg ttccgatagg tgacgacaac ctcgccgttc
     2161 gggcggcgaa agtttttttt gcggcgacga agaccaaacc tgcggtctca attgagttga
     2221 cgaaacgaat tccgtatggc gcgggtctgg gcgggggaag cagcgacgcg gcttccacgc
     2281 tgctcgcgct caacgaattg ttcgagacaa aattgccccg tgaagcgctg gcgaaacttg
     2341 cggagccaat cggatcggac gttccattct ttatctttga atcggccgcg atttgccgcg
     2401 ggcgagggga gctggtggcg ccagtgcggt tcccagagaa gctgtcgatc ttgctgttga
     2461 agcccgcttt tgccgtggca acgggatggg catattcgcg ctggaaggac tcgatagaaa
     2521 ttccaggggc cccttacgca gcccaggaat tcgctggtca tacttttgtg aatgaccttg
     2581 agcggcctgt gatcgaaaag tttgtttttc tggcgcaact caagacgtgg ttattgagcc
     2641 agtcggaggt gggcgcggcg ctgatgtccg gttctggttc gacggtgttc gcggtgatgc
     2701 gcgaaaacgc ggatgtcgat ctggtggcga aacgcgccaa agccgcgctc gacccggagc
     2761 tttggatctg cgcgtgcaat acgattgggt agcgcatgcg tctcgcgtgc tggttcggcg
     2821 tcgcgccgaa acagtctttc cttccatttc gctagcagtg aagatgagcg ggcaaaacaa
     2881 agtccgcgac cgcgaggacg cgctcgccag cacgcgagac gcatgcgcta cctcagatca
     2941 atccgccgcg cggcctgtat tattcgcccg cgccgcgttg gatcgccgcg cgtgcgtatt
     3001 cgcgattcaa tttcgcgatg aagctggcgc tgatctcagc ggggcaaacc gcttcgcact
     3061 cgtaggtgtt ggtgcagttg ccgaatcctc ccgcatccat cacgcgcacc attttcaaaa
     3121 cgcgccttgc ccgctcagga tctccctgcg gcagcagcga taaatgtgac actttcgccg
     3181 ccgtgaatag catggccgat ccatttggac aagccgccgc gcacgctcca caaccgatgc
     3241 aggccgccgc ttccattgcg agatcggaat cgtgcttggg aatcggcgtc gagttcgcct
     3301 ccggcgccga accggctcgg gcggaaatga atcctccggc ctgcacaatt cggtccagtg
     3361 cgcttcgatc gattacaagg tccttgatga tcggaaatga tctgacgcga aagggctcga
     3421 ccgtaattgt cgcgccgtcg cgaaactttc gcatgtaaag ctcgcagacc gcgccgggat
     3481 gatcggggcc gtgcgcttcc ccgttaatgg tgagcgagca ggtgccgcaa atcccttcgc
     3541 gacaatcgga atcgaacgcg attggctcgt cgccccgccc gagcaatttt tcatttagaa
     3601 tgtcgagcat ctcgagaaac gatgtgttcg gcgaaatgtc gcgcgcttcg taatcgacga
     3661 gtttgcccgg agctctcggg tttggctggc gccagattct cagtttgaag ttcatcgatt
     3721 cacgtcatcc tgagcgcagc gaagcgaagt cgagggatcc cctggagata cccaccggtt
     3781 tcgcaacggg attcctcgac ttcgctcgga acgacagcgc ggtaatcatt tatagctcct
     3841 ctgcgtcaga tgcacggttt cgaaatgtag cggttcacga tgcagtcttg gtcccgcagt
     3901 cgcgggatcg ccttgaaact cccacgcgaa cactgcggcg tggttttcgt cgtcgcgcag
     3961 cgcttcgcct tctggcgttt gatgttcttc acgaaaatgg ccgccgcatg attcaccacg
     4021 ggcaagcgcg tccgcgcaca tcaactcggc aaattcgagg aaatcggcca cgcgtccggc
     4081 acgctcgagc gattggttga aatcttcgtc gtcaccgggg acgcgcactt cgcgccagaa
     4141 cttctctcgc agttcgggaa tcgtttgcag cgcttcgtgc aatcctttgg catggcgggc
     4201 catcccgcat ttgtcccaga gaagcaagcc aagttcgcga tggatggaat cgagcgaacg
     4261 ttttccatcg acatcgagca acctccggat gcgcgcgcgg acctcgctct ctgtcttttg
     4321 aaattccgga gaatcggctg atggacgcct acccatctga ccggcgagat aattcggcaa
     4381 agtgtagggc aaaatgaaat aaccatcggc cagaccctgc atcagtgcgc tggctccgag
     4441 ccggttcgcg ccatgatcgg aaaaattcgc ttcgccgata acgtgcagcc cgggcacgtt
     4501 gctcatcaaa ttgtaatcca cccagagacc acccatggtg tagtggactg ccgggtaaat
     4561 ccgcatgggc actttataag catcttcacc ggtgatcttt tcgtacatct cgaacaaatt
     4621 accgtagcgt tcgcgaattg tatctccccc gagtcgcttg atcgcgtcgg caaaatcgag
     4681 atagacgccg cgtccgccag gtccgacgcc gcggccttcg tcgcaaactt ccttggccga
     4741 acgtgatgaa atatcgcgcg gagcgagatt gccgtagctc ggatattttc gctcgagata
     4801 ataatcgcgg tcttgttcgg aaatttcgct gggcgccttc tcgcgatctt cctttcgttt
     4861 cggcacccag acgcgtccgt cgttgcgcag cgattcggac atcagcgtca actttgattg
     4921 gtgttcgcca ctcaccggga tgcaggtggg atgaatctgc gtgaaggagg gattggcgaa
     4981 gagcgcgcct cgtttgtagg cgcgataggt ggcggtcaca ttgcagccgc gggcgttggt
     5041 cgaaagataa aaaacgtttc catatccgcc ggtcgcgagg acgacagcat cgcctgcgtg
     5101 cctttcgatc cgtccagagc gcaagctgcg cgtgatgatt cccttggcgt gtccatcgac
     5161 gatcaccaga tcgagcatct cggtgtacgg aaacatctgg accgtgccgg ccgcgatttg
     5221 gcgacaaagc gcctggtaac aaccgagcaa aagctgctga ccagtctggc ctcgcgcgta
     5281 aaacgttcgg gatacctgcg cgccgccgaa agagcggttg gcaagaaggc cgccatattc
     5341 gcgggcgaac gggacgccct gttcgacgca ctgatcgatg atcagattgc tcacttcagc
     5401 gaggcgatag acgttcgctt cacgagaacg aaaatcgccg cccttgatcg tgtcgtagaa
     5461 taatcgatac acgctgtcgc cgtcgttctg gtaatttttc gccgcattaa tcccgccctg
     5521 ggctgcaatg gaatgggcac gccgcggaga atcctggaag caaaagcatt ttactcgata
     5581 accgagttca cccagcgtcg ccgcggcgga tgcgccggcg agaccgctcc cgacaacgat
     5641 aatgtcaaac tttcgccgat tgtttggact gaccaatcgc gagtgatcac gatggctgcg
     5701 ccatttctgg tcgagtggtc cgtggggaat tttcgcgtcc agcgtcccga tcataattgt
     5761 tgcgcgggtt taaccaaacc gagcaggatc gcaatcggga tcgacgtgta tccggcaaaa
     5821 agaagccagg caaagattcg cccgccaact gccagccgcg gtgtcagttt tttgtcattc
     5881 aagccgagtg attgaaaaaa actggatgat ccgtggctca agtgcagggc gagtaaaaac
     5941 aagccaagca agtagaagcc ggacacaaga taactttgga aaccgtaaac catcatggag
     6001 taaacgtcgt aacggccaag tggatcagcc ttgagcaggc caaagcgcgg atcagtcaca
     6061 cgaacggtga aatgcgccag atggtaaatg atgaaggcca gcacgatcag cccgctcata
     6121 accatgtgcc gtgaggcgaa cgtggccttt acgtattgcc tgtcgatgta ccggtcaggc
     6181 cgtgcgctcc ggttttcaat cgccaattga atcgtcacgt aaatatgaac gatgactgtc
     6241 aggagtaaaa aaatgcgaat caaccacagc agcgggccga ggtcgcgcag atgctgagag
     6301 taaccgttga tccagtcagg tccgagaaag atttgcagat tgccgagcaa atgtccgaga
     6361 acaaaaagga tgagaatgat gccggtgatc gcgacgatca ttttcttgcc aaccgacgag
     6421 cgataaaagg ccactagaat attcacgtca gattttgtgc acccgaagga attgtgcacg
     6481 cgtttgagat tatggacgcc gcttggagcg tcaagaacgc tctagcggca tgcatgtctc
     6541 ccgacgcccg ctctttcagg gct
//
LOCUS       DASQUQ010000011        18152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_812467, whole genome shotgun sequence.
ACCESSION   DASQUQ010000011 DASQUQ010000000
VERSION     DASQUQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 18152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18152
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812467"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VE486_00310"
     CDS             complement(<1..575)
                     /locus_tag="VE486_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZA37558.1"
                     /translation="MATIPVEGGRIACRRIGSGRPLVILNGLAATSADWDPSFIDRLA
                     SANELVLLDNRGIGASPDDGAPFDIDQLADDTARAIEALEFERVSVLGWSMGGFIAQT
                     LALQHPTRIDKLILLSTDPGGVDAELASPTVRSQLTDTSGTPREQARRLLSLLFPPVL
                     ADSIYRQFGDIVANARAQLSPDLVKRQAAAM"
     gene            686..2668
                     /locus_tag="VE486_00315"
     CDS             686..2668
                     /locus_tag="VE486_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37559.1"
                     /translation="MGNSNSTGQLAWQRNPIFEFFASLKLAVVLLAVLIIAAIAGTIY
                     ESSFDAKVARAYVYGAPWFNLWLVLLAANLTASAFSRWPWRKHHVAFLITHLGIITLL
                     TGSLIGRIWGIEGTITLFKGDPPTNRLLVDQRQLRVHDVDGIVKGFPAEFLHHPPTPQ
                     RPRDLGLLASGAHLQIVDYAPAIESKLNPKPVSDRGAPALHFTINTAMMNQHLDGWLL
                     ADDPQHGNFAMGLATIEFKRGAANATKETDETHASDAPHAAVELEESIFAFSKAPDEQ
                     IGHVRKGGSTGAKVRLLAPEKGTKGRVVISSGASETSFDVAETLGRDFEIAGTPFTLR
                     IENYWPDFRIENGKPSSLSEEPNNPAVLVTIRGKGVPAITTAPNPHGKDLPTTGGPPT
                     MPAPGEQAPNHLTLFIGDDGALSYNLVSRKAGTSSGKIEIGEPLTTGWADWQLKIDKV
                     MPHAEQWMDFVPAKGEQTRDLPDGVRIRVVQNGKTFEQWFPAGWQIPVPTSPNETQIA
                     YGWKAVALPIALELLDFEVKRNEGSDNPASFKSTLRIVTVDGETATGECWMNNPFSYP
                     GQWWRTWSGLTYKISQASWNPENLNQSTVQILRDPGWLLKWIGSLLVVIGVFMMFYLQ
                     PYRKQTEGEPITLATSTEQKRRSQRPLVSIGPEANR"
     gene            2753..5773
                     /locus_tag="VE486_00320"
     CDS             2753..5773
                     /locus_tag="VE486_00320"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZA37560.1"
                     /translation="MKTSKVLFAGIFLTPIVIQFVANSESSRFAPSQQGNTPGGATTQ
                     QLSPAFTFNVLATTAEAPLALRFFSTAVKTDSRGRIITGPTQDPVKNIVGPEAAGQRA
                     GLMRFTKIMFGAAYVDGRPDPGTRIDPNNPRVTSGNQVWPNRPQPFRSHPRALSITPD
                     GRKLYVALPGREGYPDWRVAVVDTVQRRVLRWIDLRPAGQSRGLRPIGVKVSPLNASI
                     YPRPYAVALNQYGNFATVIDTGTDSVIGHFETGFYGEKALFNGTGTRLYITDRANDQV
                     RAFRVDPGPIFTQIAQIPTGTTELERTNPRDLALSANGNTLYVANTLGHTVAAINIAN
                     DANTLTRVLPVGGLSTDVKVAGRWGIVCGQNTNTALNGRETGHGLPTRNAQGVVIKND
                     GTALGYTPVMIDATKATTFDDIGSELNIFDTSTNSFVFRYVDKGRDFSQLVRPLEVVD
                     LHDHTAGQRLIRGSGPEQMVIKNGMLFVTMLHSDRVEAFRINQQPGDPSQILTPLGFE
                     FTGGITPQGVEATPDGRTVYVANMQTEDVSILSVDTNGNFTRNGFIAVGVTPSTPDPT
                     RGNNGAGLFATDEEVGLRWFFSSAYADDPPLAGWTPGGFDAQKSCGFCHWDGRHDGCQ
                     WNVAANAVGGVKVCPQNKDISDNWPEWYEGLNNDFMAYASACNGEVLGAERNPTVLFP
                     QAAQSDRFHAREDYVLRKTEENSNAIGRPELNGDAKKLGYHRMAYLQILWSQNETRRL
                     PNPLAQFPDPAIANQITRGRQIFTNGVAQGGAGCAECHHNGNKFTNGVLDDSFQDFNI
                     HEPGVISESTVDGKGPFFRPADDYFFVEFAPPQDVGTPQNFASRNTKHLRAFWDAVPR
                     YLHYGFAHNTREILLTPDSPLLRPGERGSNFRTVRTDHSRATGVGYQGSQPPVLPTQV
                     PITFGDTSGSLGGDGNGPVYVSLDSPFIQLQDGTLQIDRLGTSNVAPLLVGGQINPAL
                     NQNHINVIKDTHGKTSQLSASDVDALVLYLKSLQ"
     gene            5886..6542
                     /locus_tag="VE486_00325"
     CDS             5886..6542
                     /locus_tag="VE486_00325"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HZA37561.1"
                     /translation="METRKGIQLFALVSLVAVLGACASSKQSDRVSDAERPASGGSKW
                     GYFNGEPVTRWNPDGRTMTLLNELRYTDPQGFTWVAPAGSEVDGASIPRYLWSIMGGP
                     FEGKYRKASVLHDVAYGQKNRPWQDCDRMFYYAMRCGGVSAVEAKTMYYALYRFGNHW
                     KFPIKRARAVKVGRATVARAIPRAIPVNPNEINEAHDWIQRAEPSLEQIEQRAEAEGL
                     "
     gene            complement(6647..7390)
                     /locus_tag="VE486_00330"
     CDS             complement(6647..7390)
                     /locus_tag="VE486_00330"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZA37562.1"
                     /translation="MSAGSLLRGVGNKGYEHAFPIYRLLYRAFKVYTDRAERQLLRKI
                     LFPGAVVVDAGANIGIYSEFLSHCVGPTGAVHSFEPSPDNFRRLRAATRKFPNVRLSQ
                     AAVGEHSGSSELYLSDKLNVDHRVYIADGGSRRAVPIEIVALDDYFKPGQRVDLIKMD
                     IQGYELHALRGANRILEDNPDVKLLIEFWPYGLKQAGANWANLIAALEQKGMVIRQVS
                     SEGLIPFYPGSTSESADWYVNLFASRTSR"
     gene            complement(7390..8172)
                     /locus_tag="VE486_00335"
     CDS             complement(7390..8172)
                     /locus_tag="VE486_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZA37563.1"
                     /translation="MSGATAWESEEARDRATTLRNRARLEANRNLLFWYRELYRDQFK
                     DLPNPEALSILEIGSGTSPLKQFHSNIVTSDILHLDYLDLVFDCHEIDRLNAIKDNSL
                     DVITLTNVLHHLKSPIAFLNRAAGKLKPGGKVIATEPFFSVLSTPIFKYLHHEPVDLR
                     ISEPELGQVQGPLASANIALPWLIFFRKPAWLQRLSENFDVASRSIRPFTALSYMATG
                     GISHRLPVPGFLYRALFPIDLALSRYFPRLCASFFTVTLTRR"
     gene            complement(8169..8891)
                     /locus_tag="VE486_00340"
     CDS             complement(8169..8891)
                     /locus_tag="VE486_00340"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZA37564.1"
                     /translation="MQERIAKEIQHGRFLAEHGAGEIWNWESPAGKLRWARRVKMLSG
                     HLKPEMTVLELGCGTGYFTRELARSGADIVAIDVSPELLEIARTNCSAPNVRFEIQNA
                     YALTYSNATFDSVVGSSVLHHLEIEEAVREIYRVLKPGGTIHFTEPNMLNPQIAIQKN
                     VPWIKRRLGDSPDETAFFRWSLRRLLEQTGYGDVQIDPFDFLHPKTPVPLIDPLNAFG
                     SFLEKTPVVSEFAGSLYIRAVK"
     gene            complement(8955..10547)
                     /locus_tag="VE486_00345"
     CDS             complement(8955..10547)
                     /locus_tag="VE486_00345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA37565.1"
                     /translation="MNGAGIEGSCDAKLRSGESAKGDLRPGGIDNGNMEAVLKEREVN
                     REVWGPSALEQRRERTRKHLAGIASRRENWIKRNRYYYELLSRLLRFLVEPEKKVLSV
                     RCDTGNLLSAVRPGDGKGIEICSEIVEIAQQRNPSFNFAVAFPDKDEFRKLFSQDDKF
                     DYILFNDIGDTVDVLQALRNLRPLCRRQTRLLVTTYNHLWEPLVTFAEWIGMKVPRTE
                     QNWLSTADIRNLMKLAGFEALEMHWIVLLPKYVPLLSAFLNRFCARLPFLKRLCMTQI
                     VVARAVPPPVPKEELSVSVIIPCKDERGNVEDAVRRMPQLGGQTEIIFCDDQSTDGTA
                     EEVMRARARYPEQHIRLEHGPGVCKSKNVWTGFNAATGDVLMILDADLTTMPEELVYF
                     MEVIASGQAEFVNGSRLVYPVPKGAMNGANMLGNKFFSVAFTYLLGQRVKDTLCGTKV
                     LWRSDWERIRPMVGRWGTEDRWGDYELLFGAAKLNLKILDLPVHYQERIYGSTKMTKV
                     FRNGLVMLRMCWHGFLKLKLGY"
     gene            10846..11811
                     /locus_tag="VE486_00350"
     CDS             10846..11811
                     /locus_tag="VE486_00350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZA37566.1"
                     /translation="MSANKAQEIIKRAMADRSVYDAMAARENEVWGKILPDLERSEAR
                     MEDAEASAELTVARHTSSLLRVARERNLKFENGLTLGCGAGRRERELVNAGVCRTFHG
                     IDISEKAIAAARDLAKEQDLPLTYEVADLNFVELPEKAFDLVLAQTALHHVLFLERVA
                     EQIWRSLKSDGYLWIHDFIGETQGQYDPKRLSIMNRILAILPEKFRQNKINGQLIAEI
                     KRPEPGRLGSPFESIRSGEIIPVFQRWFTIEWQMEFDAILRLVMPPGTRAAYLENEDT
                     KALFEILMLLDRLCIEEKVVQPVGGQYLMRPRSVNDIPAKSTTNG"
     gene            11777..12607
                     /locus_tag="VE486_00355"
     CDS             11777..12607
                     /locus_tag="VE486_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZA37567.1"
                     /translation="MISPQKARQTDNAPDSAHRTGDSGLYAAPTLDVDPQSAATYHRI
                     DVPGLEDKKWQWDLRGQERSYLGNYDFNGKRVLEFGAANGGLTFWMEQQGAKVIAVDL
                     SPAVAEWDNLLGPEDNLRDIRHLMARGIRRLNNGFWYTHERLGSKARLVHGTAYNVHN
                     KIGRFDVVTLCSILIHLRDPLRALENAISFAEGSVIITDVVPRIIAKHLQPLPLAYFV
                     PDKSRRVPHGGWTWWHITAEVYVRFLSLRGFRVVSNASAFYKHKSGPRELYTLVAQRM
                     "
     gene            12735..15755
                     /gene="uvrA"
                     /locus_tag="VE486_00360"
     CDS             12735..15755
                     /gene="uvrA"
                     /locus_tag="VE486_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZA37568.1"
                     /translation="MPPEAIKISGARQHNLKNLHLEIPREKLVVITGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLDQMEKPDVDFIEGLSPAIAIEQRSAGANPRSTIAT
                     TTEVYDYLRVLFSAVGQPHDPLTGRPLKRQTPQQIVDQILSYEPGSKIIVLAPLIENQ
                     PGEFRDVIEKTKREGFVRVRIDGRIIELDRSEPIRLKKSERHTIEAVIDRLMVREGIR
                     VRLTDSIETALKWGGNRVVVLRQRCGDGVMEKWSNGKFTAPTLQNSNAPSGDWEERRY
                     STAYGNAETGFTLGELTPKHFSFNSHLGACPACHGLGTQLVVDPDLMISDSTKTLAEG
                     AITPWRRGTKRMRAYYRHLQGALVKHFHVDENVPFADLPSDFKTALYFGTNGQPIEMN
                     SAGKEEKRVTKPFEGLVPQMQRLYEQTESEFTRNRIRAFMTREPCKICGGARLKPEIL
                     GVTIRGAKELNIHQFSEQTIEAAVRFMGALDLTAQQQVIVDDVVREIQLRLQFLVEVG
                     LGYLTLNRESGTLSGGEAQRIRLATQIGSGLAGVLYILDEPSIGLHQRDNARLLGTLR
                     RLRDLGNSVIVVEHDEETIRAADHIIDLGPGAGPRGGEIVAQGSIAEVLRAKNSLTAD
                     YLSGRARIAIPKQRVEPRGHSERSRGTPQNGSRGTSRDVSTSLDMTRTEEGWLTVVGA
                     NENNLKKIDVSFPLGCLTCVTGVSGSGKSTLVDDIFRRVLFRHFYNSKERPGAYQAIR
                     GLDQIDKAVVIDQSAIGRTPRSNPVTYTGAFTPIRELFSQLPAARVRGYDAGRFSFNV
                     KGGRCENCEGGGLIKIEMHFLPDVYVECEVCHGKRYNRETLEITYKGRNIADVLDLTV
                     DEAARFFRNVPSISEKLNSLLDVGLGYLRLGQAGTTLSGGEAQRVKLATELAKKATGR
                     TVYILDEPTTGLHFADIEKLLQVLMKLRNAGNTVIVIEHNLEMIKCADWIIDLGPEGG
                     ERGGEIVGTGPPERIVDIPNSFTGKYLRPMLEKK"
     gene            15752..>18152
                     /locus_tag="VE486_00365"
     CDS             15752..>18152
                     /locus_tag="VE486_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA37569.1"
                     /translation="MSRIRRIGLIGLIALVAQPLRAEISPELAEASAPLTDGVPEVAV
                     ARLEKLLNRNLPDPEWHAVAEKLAEAHVGAKQAEAALVLLSDSRLRELPWAKFWRAQA
                     FASLHRWADALPLYEELEADETSSFRKAATFGAAEMLRALGRPDEALTKFRSLTRDKE
                     WATRAQLRSAKLYIDKGDAINARRVLDDMQPKSVGEQNDRRLLRGRLELVLQRPEKAM
                     SVFEELLKTPEKVSHATLIAALFGMVDAHVQLKTPDAGADLIEEFINRYPADPDLPAL
                     FARLDELYRAVRKPSRSELEKWVRNPEQPRRTFARWYLARLENRAGRRDRARQLFRDV
                     RRADVKPPEVAAALLEFAQLEVEDWNFDEAIAILDDARLLHPEPSVVDRINLLAAKTQ
                     FLAKRFEVATAGFEQIGHSNSPWAKIALLNASAGWLQLGDHERFAANYNELETRGVDE
                     ESRANLRLEEALMQAAKGDNKAAKALQQFIREFPKSPRVSEAWVSLAELAYHSSPPRI
                     DEARTNLARALDSTPTAAATERADYLMIWIEDSNGADEIRVIDLAKRFLQQHGGSAFA
                     PEVRMKLAEIYYRRQDFANAQTQFEILAKENPDNRMGENALFFAAESAMSSMGEGSLD
                     RAIVLFDQVVRLNGPLRWAARNEQAVIERKLGKPQDALVLYDEVLKSDAGLPEKREAL
                     CGKGDIFFEMGGSRPENYQRALEIYEQLASGKDTPIHWRNQALFKKALCLEKKNDRPG
                     ALATFYKVLEDEARPDRRSELFWYYKAGFNAARLLEDDSKWESAAAVYEKLAAAGGSR
                     SE"
BASE COUNT         4164 a         5001 c         5166 g         3821 t
ORIGIN      
        1 tccatcgcgg cagcctgccg cttaacgagg tcaggagaaa gctgcgcgcg cgcgttcgcc
       61 acgatgtcgc caaactgacg gtaaatagaa tcggcaagaa ccgggggaaa caatagggag
      121 agcagtcgtc gagcttgctc acgcggtgtg ccagatgtgt cggtgagctg cgatcggaca
      181 gtcggagatg cgagttccgc atcaactcct ccggggtcgg tagagagaag gattagtttg
      241 tcaatgcggg tcggatgctg caaagcaagc gtctgggcga tgaatccccc catcgaccat
      301 cccagcacgc tcacacgctc gaactccagc gcttcgatcg ctcgcgcggt gtcgtccgcg
      361 agctggtcga tatcgaatgg cgctccatca tccggggaag cgccaatgcc gcggttgtcg
      421 aggagaacca gctcgttggc agacgccagc cggtcgatga acgagggatc ccagtcggcg
      481 ctggttgcgg ccaggccgtt gaggatcacc agcggtcgcc cgctcccaat ccgccggcag
      541 gcgattcgtc cgccctcgac tgggattgtc gccatggact cactggccat cggcgaccat
      601 cggaagccgc tttgcgagcg tcaagtccaa gcgcgcgatc caccttgaac gcaacattat
      661 cctcgcggaa ggtgtcgatc ttcgcatggg gaactcgaat tcaaccgggc agctcgcctg
      721 gcaacgcaat ccgatcttcg aattttttgc gtcgcttaag ctcgcggttg ttctgctcgc
      781 ggtgctcatc atcgctgcga tcgcaggcac gatctacgaa tcgagtttcg atgcgaaagt
      841 agcacgcgcc tatgtttacg gggcgccatg gttcaatctc tggctcgtgt tgctcgccgc
      901 caatctgacc gcatccgcgt tctcacgctg gccgtggcgc aaacatcatg tggcgtttct
      961 catcacgcat ctcggcatca ttaccttact gaccggatcg ctcatcggcc gaatttgggg
     1021 gatcgaagga actatcacgc tgttcaaagg cgatccgcca accaatcgtc ttcttgtcga
     1081 tcaacgccag cttcgtgtcc acgatgtcga tggaatcgtg aaaggattcc ccgcggaatt
     1141 tttgcatcat ccgccgacgc cgcagcggcc acgtgatctc ggtctgctcg ccagcggagc
     1201 gcatttgcaa attgtcgatt acgcgccggc gattgagagc aagctgaatc cgaagccagt
     1261 gagcgaccgc ggcgcgccag cgctgcattt cacaatcaac acggcgatga tgaaccagca
     1321 tctcgatggc tggctgctcg ccgatgatcc gcaacacggg aatttcgcga tgggtctggc
     1381 gaccatcgaa ttcaaacgcg gagcggcgaa tgcgacgaag gagacggatg agacacacgc
     1441 gtccgatgcg ccccatgccg ctgtcgagtt ggaggaatca atcttcgctt ttagcaaagc
     1501 gccggacgaa cagatcggcc atgttcggaa aggcggcagt acaggcgcga aagtgcgatt
     1561 gcttgcacct gagaagggaa ccaaaggtcg agtggtaatt tcttcgggcg cgagcgagac
     1621 cagcttcgat gtcgcagaaa ctctcgggcg cgacttcgaa atcgccggaa cgccattcac
     1681 gctcaggatc gagaattact ggccggattt ccggatcgag aacggcaaac cgagttcgct
     1741 gagcgaggaa ccaaacaatc ccgcagtgct tgtcacgatc cgcggcaagg gcgttcctgc
     1801 cataacgaca gcgccgaatc cacacggcaa agatttgccg acgaccggcg gcccgccaac
     1861 gatgccggcg ccgggcgaac aagcgccgaa tcatctcacg ttgttcatcg gcgacgatgg
     1921 cgcgctttcc tacaatctag tctcgcgcaa agcgggcacg tcgagcggca agatcgaaat
     1981 cggcgagccg cttacgacag gttgggccga ttggcagctg aagatcgaca aagtaatgcc
     2041 gcatgccgag caatggatgg attttgttcc ggcaaaagga gagcagacaa gggatttgcc
     2101 cgacggcgtt cgcattcgag tagtgcaaaa cggaaaaaca ttcgagcaat ggttcccggc
     2161 cggatggcaa atcccggtgc cgacttcgcc taacgaaacc cagatcgcct acggctggaa
     2221 agctgtcgcg cttccaatcg cgctggagct gctcgatttc gaagtaaaac gcaacgaggg
     2281 cagcgacaat cccgccagct tcaaaagcac gctgcgtatc gtcacggtcg acggcgagac
     2341 tgccaccggc gaatgctgga tgaacaatcc atttagttat ccgggccaat ggtggcgaac
     2401 gtggagcgga ttgacgtaca aaatctcgca agcgtcatgg aacccggaaa atcttaatca
     2461 aagcaccgtc cagattctgc gcgatcccgg ctggcttttg aaatggatcg gttcgttgct
     2521 cgtggtgatt ggcgttttca tgatgtttta ccttcagccg taccggaagc agacagaagg
     2581 cgagccgatc acgcttgcga cgagtacgga gcagaagcgt cgttcccaaa gaccccttgt
     2641 ttcaatcggg cccgaagcca accgataaag gttgccgtgc ccgtgaggta acaatcgtgt
     2701 aaccattttg cactcgcgcg caaaatgcat ggctgacacg attttttttg tcatgaaaac
     2761 atcaaaggtt ttgttcgcgg ggatcttttt aacgccaatc gtcattcaat tcgtcgcaaa
     2821 cagcgaatcc agtcggttcg cgccttcaca gcagggtaat actcccgggg gggccaccac
     2881 tcagcagcta tcgcctgcat ttactttcaa cgtgctcgcg acgacggctg aggcgcctct
     2941 cgcactccgc tttttctcca cagcagtcaa aaccgattct cgcgggcgaa tcatcactgg
     3001 gccgacccag gacccggtga aaaacatcgt tggcccggag gcggcgggtc aacgcgctgg
     3061 gctgatgcga tttacaaaaa tcatgtttgg cgccgcctac gttgatgggc gacccgatcc
     3121 cggaacgcga atcgatccta acaatccgag ggtaaccagc ggaaaccaag tctggccaaa
     3181 tcgcccgcag ccgttccgaa gccatccgcg tgcgttgtcg atcacgccgg acggcagaaa
     3241 gctgtatgtg gcgctgccgg gacgcgaagg gtatcccgat tggcgggtcg ccgttgtcga
     3301 tactgtacaa cgacgcgttc tgcgctggat cgacttgcgg ccggccggcc aatcacgcgg
     3361 gttgcgtcca atcggtgtga aggtttcgcc gttgaacgcg tccatttatc cgcgtccgta
     3421 tgcagttgcg cttaatcaat acgggaactt cgccaccgta atcgacaccg gaaccgattc
     3481 cgtgattggt catttcgaaa ccgggtttta cggcgaaaaa gcgctgttca acggcactgg
     3541 cacgcggctc tacatcaccg atcgggctaa cgatcaggtg cgcgcgttcc gcgtcgatcc
     3601 gggaccgata ttcacccaga ttgctcagat ccctaccggc acgacggagc tggagcggac
     3661 aaatccgcgc gatctagcgc tcagcgctaa tggcaatact ttgtacgttg caaatacgct
     3721 cgggcatacc gttgctgcga ttaatattgc caacgatgcc aacaccctaa cgagagtgtt
     3781 gcccgtcggc gggctttcaa ctgatgtaaa agtcgccggg cgctggggaa tcgtgtgcgg
     3841 ccagaacacc aacacggcgc tgaatggccg cgagaccgga cacggcctgc cgacacggaa
     3901 tgcacagggc gtggtgatca agaatgatgg cacagcgctc ggctacaccc cagtgatgat
     3961 cgatgccacc aaggcgacga cattcgatga cattggaagc gagctgaaca ttttcgacac
     4021 cagcaccaat tcgtttgtct ttcgatacgt ggacaaagga cgcgattttt cacagctggt
     4081 taggccactc gaggtggtcg atcttcatga tcataccgcg gggcagcgtt tgattcgcgg
     4141 cagcggtccc gaacaaatgg tgatcaagaa cggcatgctt ttcgtcacca tgcttcactc
     4201 ggacagggtt gaggcgttcc gaatcaatca acaaccgggt gacccctcgc aaatccttac
     4261 ccctctcggt ttcgagttta ccggcggcat cacgccacag ggagtagagg ctacgccgga
     4321 tgggcgcacg gtttacgtgg caaacatgca aaccgaagac gtctcaatcc tgtccgtcga
     4381 tacgaatgga aatttcaccc gcaacggctt catcgccgtc ggagtgacgc cgagcacgcc
     4441 ggatcctacc cgcggcaaca acggcgctgg cctgtttgcc acggacgaag aagtcggttt
     4501 gcgctggttc ttcagctcgg cctatgcgga cgatccgccg ctggccggct ggacacccgg
     4561 cggattcgat gcgcagaagt cgtgcggttt ctgtcattgg gatggccgac acgatggctg
     4621 ccaatggaat gtcgctgcga acgcagtcgg cggcgtaaaa gtttgcccgc aaaacaaaga
     4681 catctcggat aactggcccg aatggtacga aggcctaaac aatgatttca tggcctacgc
     4741 ctcggcctgc aatggcgaag tgctcggcgc ggagcggaat ccaaccgtgc ttttcccgca
     4801 ggctgctcaa agcgatcgct tccacgcccg cgaggattac gtgctccgga aaaccgagga
     4861 aaactcgaac gcgattgggc gtccggagct aaacggcgat gccaagaagc tcggctacca
     4921 tagaatggcc tacctgcaaa tcctgtggtc gcaaaatgaa acccgccgct taccaaaccc
     4981 gctggcgcaa tttcccgacc ccgcgattgc caaccagatt acgcgcggta gacaaatttt
     5041 cacaaacggg gttgcccaag gcggcgccgg ctgtgcggag tgccaccaca atggcaacaa
     5101 attcactaat ggcgtgctgg atgattcatt ccaggacttt aacatccatg agccgggcgt
     5161 gatcagtgaa tcgacagtgg acggtaaagg cccgttcttt cggccggcgg acgattattt
     5221 ctttgtcgaa ttcgctccgc cgcaggatgt cggcacaccg caaaacttcg ccagccggaa
     5281 cacgaaacat cttcgcgcct tttgggacgc tgtaccgcgt tatcttcact acgggtttgc
     5341 tcataacacg cgcgagattt tgcttacacc cgattcgccg ctgctccgac cgggcgagcg
     5401 cggctcgaat tttcgcactg tgcgcaccga tcactctcgc gccacgggag tcggttatca
     5461 gggctcgcaa ccacccgtgc tgccgacgca ggtgccaatc acatttggtg acaccagcgg
     5521 aagtttaggc ggtgacggca acggtcctgt ttacgttagt ctcgacagtc cgttcatcca
     5581 gctgcaagac ggcacgctgc aaatcgatcg tcttggcaca agcaacgtcg ccccgctgct
     5641 agtcggtggc cagatcaatc ccgccttaaa ccaaaaccac atcaacgtga tcaaggacac
     5701 acacggcaaa acgtctcagc tttccgcttc ggacgtggac gcgcttgtgc tctatctgaa
     5761 gtctctccag tagtgcgtcc ctgacgtact tcattggtcc cgttcttttt ctgcagcgga
     5821 gaggacgcga agcgccgcgt gagggttgaa tgttgagcat tgagcgttga acgttctgct
     5881 ctcacatgga aacgaggaaa ggaattcaac tgttcgcgct cgtcagtctg gtcgccgttt
     5941 tgggagcttg cgcatcaagc aaacaatcgg acagggtttc cgacgctgag cgacctgcct
     6001 cgggaggatc caaatggggt tacttcaacg gcgagccggt cacgcgatgg aatccggatg
     6061 ggcggacaat gaccctcctg aatgaattgc gttataccga tccgcaaggc ttcacctggg
     6121 tcgcgcctgc gggatcggaa gtcgatggcg catcaattcc tcgttacctc tggtccatca
     6181 tgggcggccc gttcgaagga aaatatcgga aagcttccgt attgcatgat gtcgcttacg
     6241 ggcaaaagaa tcggccgtgg caggattgcg accggatgtt ttactacgcc atgcgatgcg
     6301 gcggcgtcag cgcagtggaa gcgaagacga tgtattacgc cctgtatcga ttcggaaatc
     6361 attggaaatt tccgatcaaa cgcgcaagag cggtgaaggt cggcagagcg acggtagcaa
     6421 gagctatccc gcgagcgatc ccggtgaatc cgaatgaaat caacgaagcg cacgattgga
     6481 ttcaaagggc cgagccgagt ctggaacaaa tcgagcaacg cgccgaagcc gaaggccttt
     6541 gattggtctc tacgaacttc acgccttgcc gaatgactta cgcgagtcag tccccttcca
     6601 tgtctggcgc aggcccggca ccgttctcca gcgcagggtt aagacttcat cgactcgttc
     6661 gcgaggcgaa tagattcaca taccagtccg cgctctcact tgtagagcca gggtagaacg
     6721 gaatcagccc ctcgctcgac acttgccgga tgaccatgcc tttttgctcg agagccgcaa
     6781 tcagattagc ccagttcgcc ccggcctgtt ttaatccgta gggccaaaat tcaatcagaa
     6841 gctttacatc aggattatcc tccaaaatgc gattggcgcc tctcaaggcg tgaagctcgt
     6901 atccttgaat gtccattttg atcaggtcca cgcgttggcc aggtttgaaa tagtcgtcga
     6961 gcgctacgat ttcgattggc acagcacggc gcgaaccgcc gtcggcgatg taaacccggt
     7021 gatcgacatt tagcttgtcc gaaagataga gctcggagct gccgctatgc tcgcccaccg
     7081 cagcttgcga gaggcgcaca tttgggaact tgcgcgtggc agcgcgcaat cgtctgaaat
     7141 tatcgggcga cggttcgaat gaatggacag caccggtcgg gcccacacag tgcgaaagga
     7201 attcggaata aattccgatg ttggcgccgg cgtccacaac gacagcgccg ggaaacagaa
     7261 tcttcctcaa gagctgccgc tctgcacgat cggtgtaaac cttgaaagcg cgataaagga
     7321 gccggtagat tggaaaggcg tgttcgtagc ctttatttcc aacaccgcga agaagcgagc
     7381 ctgccgacat tagcgccgcg tcagagttac ggtgaagaac gacgcgcaca accgcggaaa
     7441 atagcggctg agcgcgaggt caatcgggaa taaggcccga tagagaaaac ctggaaccgg
     7501 cagcctgtgc gatattccgc cagtagccat ataagacagc gcggtaaacg gccgaatgga
     7561 gcggctggcg acgtcgaagt tctcactcag gcgctgcaac catgctggtt tgcggaagaa
     7621 aatcagccag ggaagcgcga tgttggccga ggccagcggc ccctgcactt ggccaagctc
     7681 cggctcggaa attctgagat cgaccggttc gtggtgcaaa tacttgaaga tgggagtcga
     7741 aagcacggaa aagaacggtt ccgtcgcgat cactttgccg ccgggtttca acttaccggc
     7801 cgcccgattg agaaacgcga tggggctttt cagatggtgc aacacattcg tgagcgtaat
     7861 cacatcgagg ctgttatctt ttattgcgtt caatctgtcg atctcgtggc agtcaaacac
     7921 aaggtcgaga tagtcgagat ggaggatatc gctggtgaca atgttcgaat gaaattgttt
     7981 caggggcgaa gtgccgcttc cgatctcgag gatcgaaagc gcttccggat tcgggaggtc
     8041 cttgaactga tcgcgataaa gttcgcggta ccaaaaaagg agatttcgat tcgcctcgag
     8101 acgagcgcga ttgcgcaaag tggtggcacg gtctctggcc tcttcactct cccaagccgt
     8161 tgcgccgctc atttgactgc ccggatgtat agcgagccgg caaactccga gacgaccggc
     8221 gttttctcga gaaaagagcc gaaggcgttc aacggatcga tcagaggaac cggcgttttc
     8281 ggatgcagga aatcaaacgg atcgatttgc acatcaccgt agccggtttg ttccagcaac
     8341 cgacgcagtg accaccggaa aaatgccgtc tcgtcaggtg agtcaccgag tctgcgttta
     8401 atccacggga cgttcttttg tatcgcgatc tgagggttca gcatgtttgg ctcggtaaaa
     8461 tgaatcgtgc cgccgggttt caatacacga taaatctcgc gcacggcctc ttcgatttcg
     8521 aggtgatgca gaaccgagct gccgacaact gaatcaaatg tcgcgttaga ataagtgagc
     8581 gcgtaagcgt tctggatttc gaaacgaacg tttggcgcag aacaatttgt cctcgcgatt
     8641 tccagaagct cgggtgagac atcaatcgcg acgatatccg ccccggaacg tgccagttcc
     8701 cgagtgaagt agccggtgcc gcatccgagc tcgaggactg tcatctccgg tttcaaatga
     8761 ccgctgagca ttttcacccg ccgagcccag cgcagtttgc cggctggact ctcccaattc
     8821 caaatctccc cggcgccatg ctccgccagg aagcgcccgt gttgaatctc tttagcaatg
     8881 cgttcctgca tcagaaaatt ttggaaatcg ccgcgaactt tatgctcctg tggaacccta
     8941 cggcaatcag cggcctagta accaagcttc agcttcaaga agccatgcca gcacattctc
     9001 agcatgacca gtccgttacg aaaaactttt gtcattttgg tggaaccata aatgcgctcc
     9061 tggtaatgca ccggcaaatc cagaattttg aggtttaatt tcgcggcgcc aaacaagagt
     9121 tcataatcac cccaacgatc ttcggtcccc caacgtccta ccattggcct gatccgctcc
     9181 cagtcacttc gccagagaac tttcgtgccg cagagggtgt ctttgacccg ttgcccgagg
     9241 agatatgtaa aagcgacact gaaaaatttg ttgcccagca tgtttgctcc attcattgct
     9301 ccttttggca ctggatagac cagccgcgag ccgttcacaa attccgcctg tcccgaagca
     9361 atcacctcca taaaataaac gagttcctca ggcatggtcg tcagatcagc gtccagaatc
     9421 atcaagacat cgcccgtcgc tgcgttgaac ccggtccaga cgtttttcga tttgcaaact
     9481 cctggcccgt gctcaagccg gatatgttgt tccggatagc gggcgcgcgc ccgcatcacc
     9541 tcctcagctg tgccgtcggt cgattggtcg tcacagaaaa ttatttccgt ctgcccgccc
     9601 aactgcggca tccgacgaac ggcatcctcc acatttcctc tttcgtcttt gcagggaata
     9661 atcaccgaga cggacagttc ttctttggga accggcggag gcaccgccct ggcgacaacg
     9721 atttgcgtca tgcagagtct cttcaagaac ggcaggcgcg cgcaaaaccg atttagaaaa
     9781 gccgacagaa gcggcacata cttgggtaac agcacaatcc aatgcatctc gagggcctcg
     9841 aaaccggcca gtttcatcag gttccgaata tcggccgtgg aaagccagtt ctgctcggtg
     9901 cgcgggacct tcattccaat ccactccgca aaggtgacca atggttccca cagatgatta
     9961 taggtcgtaa ccagcaagcg agtttgccgc cggcaaagcg gccgtaaatt gcgcagcgct
    10021 tgaagcacat ccaccgtgtc gccgatatcg ttgaagagga tgtaatcgaa tttgtcgtcc
    10081 tgactgaaaa gcttccggaa ctcgtccttg tccgggaaag cgactgcgaa attgaaagat
    10141 ggattccgtt gctgcgcgat ttccactatc tcggagcaga tctcgatgcc tttgccgtcg
    10201 cctggcctga cggcggataa aaggttgccg gtatcgcagc gaaccgatag cacctttttc
    10261 tctggctcga ccagaaatcg cagaagccgg cttaacagtt cataatagta tctgttgcgc
    10321 ttaatccagt tttcgcgacg ggaagcgatg cctgccagat gtttgcgcgt acgttcgcgc
    10381 ctttgttcga gggcggacgg tccccagacc tctctgttca cctcgcgctc ctttagcaca
    10441 gcttccatat tgccgttgtc gataccacct ggtcgcagat ctccttttgc cgattccccg
    10501 ctgcggagtt tcgcgtcgca gcttccttca attccggcgc cgttcaaggt tgtaattcat
    10561 tggaaccatc tggctctttt gaaaagctga gatgttggga gcacagccat cctggctgtg
    10621 gggcaggcgg gcatcttggc tgtcgagtaa gactctcagc caggcgagac gcccagttgc
    10681 ccacaggcaa gatgccttgt gctccaatcc gccgccgttc gccagctttt gcgctcattc
    10741 cattcggcat tcggcgttcg atgttctgcc cgcccatcct gagatacgcg ttgcggttga
    10801 agaaacgatg aggcacgttc tctcttcctt agggtgccct ggttcatgag tgcgaacaaa
    10861 gctcaggaga tcattaaacg ggcgatggcc gaccgaagcg tgtacgacgc gatggcggcg
    10921 cgcgaaaatg aggtgtgggg caaaattctg cctgatttgg agcgttccga agcgcgaatg
    10981 gaggacgcgg aagccagtgc ggaactgacc gtggctcgtc atacttcatc tctgcttcgc
    11041 gttgcccggg agagaaatct gaaattcgaa aacgggctca ccctcggatg cggcgccggg
    11101 aggcgcgagc gcgagctcgt aaacgcaggt gtttgccgga cttttcacgg aatcgatatc
    11161 tcggaaaaag ccattgccgc ggcgcgcgac cttgcgaaag agcaggacct cccgctcact
    11221 tatgaagtgg ccgacttgaa tttcgtggag ctacccgaga aagcattcga tctggtcctg
    11281 gcccagaccg cgcttcacca cgtccttttt ctcgagcgcg tcgcggaaca aatatggcgt
    11341 tcgctgaaaa gcgatggtta cctctggatt catgatttca tcggcgaaac gcagggacaa
    11401 tatgatccca agcgtctctc catcatgaat cggattctgg caattcttcc cgagaaattt
    11461 cgccaaaaca aaattaacgg tcagttaatc gcggagatta aacgtcccga gccggggcgt
    11521 cttggttcgc catttgaatc aattcgatcg ggcgagatca tcccggtgtt tcaacgctgg
    11581 tttacgatcg aatggcagat ggagtttgat gcaatcctgc gccttgttat gccaccgggc
    11641 actcgcgccg cctacttgga aaatgaggac acgaaagctc tgtttgaaat tttaatgctg
    11701 ctcgaccgct tgtgtattga agagaaggtt gtgcagccag tcggcgggca atatcttatg
    11761 cgcccaaggt ctgttaatga tatccccgca aaaagcacga caaacggata acgcgccaga
    11821 ttcagcccac cgcacaggcg acagcggatt atacgccgcg ccgacactgg acgtcgatcc
    11881 gcaatctgct gcgacttatc atcggataga tgttcccgga ctggaggaca agaaatggca
    11941 atgggacctt cgcgggcagg agcgttccta tctgggtaat tacgacttca acggtaaacg
    12001 tgtcctggaa tttggcgctg caaacggcgg cctgacattc tggatggaac agcaaggcgc
    12061 caaggtgatt gccgtagatc tctctccggc cgttgcggaa tgggacaatt tactggggcc
    12121 agaggacaat ctccgagaca taaggcacct tatggcaagg ggaatcaggc gcctcaacaa
    12181 cggcttttgg tatactcatg aacggctggg ttctaaagcc agattagttc atgggactgc
    12241 ttacaatgtc cacaacaaga tcggtagatt cgatgtcgtc acgctctgct cgatcctgat
    12301 tcatttgcgc gatccgctcc gagcgctgga gaatgcaatt agctttgccg aggggtccgt
    12361 tatcattacc gatgttgttc ctcgcattat tgctaaacat ctacagcctc tccctttggc
    12421 gtatttcgtg ccagacaaga gccggcgggt accccacggc gggtggacct ggtggcacat
    12481 cacggcggaa gtttatgttc ggtttctgag cttgagaggg ttcagggttg tcagcaatgc
    12541 atctgcattc tacaaacaca agtcgggacc ccgggagtta tacacgcttg tcgcacagcg
    12601 catgtaatat gcatccgaat tcgtaacaag ggcttcggtt agaatctcgc aaaagcacgc
    12661 taccaagaaa cgcgcccaac tcgacacgga gtagcgccta tgcttgactg ttcggttcga
    12721 ctctttttgt cagaatgcct ccggaagcta tcaaaatcag tggtgcgcgt cagcacaatc
    12781 tgaagaatct ccatttggag atcccacggg agaagctggt cgttatcact ggtttgagtg
    12841 ggtccgggaa gtcgtcgctt gcctttgata cgctctatgc ggaagggcag cgtcgctacg
    12901 tggaaagtct ctccgcttac gcgcggcaat tcctcgatca gatggaaaag ccagatgttg
    12961 atttcatcga aggtctttct cccgccatag ccatcgagca gcgcagcgcc ggcgccaatc
    13021 caagatcgac catcgcaacc acgactgaag tctacgatta tttgcgcgtt ctcttctcag
    13081 cagtaggtca gccgcatgac ccgctcaccg ggcggccatt gaagcggcaa accccgcaac
    13141 agattgtcga tcaaatcctg agctacgagc ccggatcgaa aatcattgtg ctcgcgcccc
    13201 tgattgaaaa ccagccgggc gaatttcggg acgtcatcga aaagacgaag cgcgaaggat
    13261 ttgttcgcgt gcggattgac ggcaggatca tcgagctgga tcgatccgag ccaatccggc
    13321 tcaaaaaaag cgagcgacac acgattgaag cagtgatcga tcggttgatg gtgcgcgagg
    13381 gaatccgcgt tcggctcact gattcaattg aaactgccct caaatgggga gggaaccgcg
    13441 tcgttgtgct tcgccaacga tgcggtgatg gagtaatgga gaaatggagt aatggaaaat
    13501 tcactgctcc aacactccaa aactccaatg ctccatcggg ggactgggaa gagcggcgtt
    13561 actcgaccgc ttacggaaac gccgagaccg gattcacgct tggtgagctc acgccgaaac
    13621 atttttcgtt caacagccat ctgggcgcat gcccggcctg ccacgggttg ggtacacaac
    13681 tggttgtcga tccggatttg atgatttccg attcaaccaa gacactggcc gaaggcgcaa
    13741 tcacaccgtg gcgacgcggc acaaaacgaa tgcgggccta ctatcgtcat ctgcaaggcg
    13801 cgctcgtgaa acatttccat gtggacgaaa atgtcccgtt tgctgatttg ccctccgact
    13861 tcaaaactgc gctttacttc ggcacgaacg gccagccgat tgaaatgaat tctgccggca
    13921 aagaggaaaa acgagttacg aagcccttcg aaggactcgt cccgcaaatg cagcggctct
    13981 atgaacaaac tgaaagcgag ttcacgcgca atcgcatccg cgcattcatg acacgcgagc
    14041 cgtgcaaaat ctgtggcggc gcaaggctca aaccggagat tctgggggtg acaattcgtg
    14101 gcgcgaaaga gttgaacatt caccagttca gtgaacaaac cattgaagca gccgtccggt
    14161 ttatgggcgc tctcgatctc actgcgcaac aacaagtgat cgtcgatgac gtcgtgcgcg
    14221 agattcaatt gcgcttgcaa tttctcgtcg aggttgggct tggctatctc acgctcaatc
    14281 gcgagagcgg caccctttct ggtggtgaag cacagcggat tcggctggcg acgcagatcg
    14341 gctctggttt ggcgggagtc ctttacattt tggatgagcc gagcatcggc ctgcatcaac
    14401 gcgacaatgc ccgtttgctc gggacgctgc gacggctgcg cgatctcggt aactccgtca
    14461 tcgtggtcga gcacgacgaa gagacgatcc gggccgcaga ccacataatc gatcttgggc
    14521 cgggcgcggg tccgcgcggc ggcgagatcg tggcccaggg cagtattgcc gaagttcttc
    14581 gcgcgaaaaa ttcgctgacg gcagattatc tctccgggcg cgcgcgcatt gcgattccaa
    14641 aacagcgggt ggagcctcgc ggtcattccg agcgaagtcg aggaaccccg cagaatggct
    14701 cccggggaac ttcacgggat gtctcgactt cgctcgacat gacgaggacg gaagagggct
    14761 ggcttactgt cgtaggcgcg aatgaaaaca atctcaaaaa aatcgatgtc tcttttcccc
    14821 ttggctgtct tacttgtgtg accggcgtct ccggcagtgg caaatccacg ctggttgatg
    14881 acattttccg gcgtgttctc tttcggcact tctacaattc aaaagagagg cctggcgctt
    14941 atcaggcgat tcgcggtctg gaccaaatcg acaaggctgt ggtgatcgac caaagcgcca
    15001 ttggccggac gccgcgttcg aatccagtca cctataccgg cgcattcacg cctatccgcg
    15061 aattgttcag ccaacttccc gccgcgcggg tacgcggtta cgacgcgggt cgcttcagtt
    15121 tcaatgttaa aggcggccgc tgcgaaaatt gcgaaggcgg cgggctgatc aaaattgaga
    15181 tgcacttttt gccggatgtt tatgtcgaat gcgaagtatg ccacggcaaa cgctataacc
    15241 gcgagacgct cgagatcacc tacaaagggc gaaacattgc cgacgttctc gacttgacgg
    15301 tggatgaagc ggcgcgcttc ttccgcaacg tgccgagcat ttcagaaaag ctgaattcgt
    15361 tgctggatgt gggcctgggt tatctgcgtt tgggtcaggc tgggaccacc ctttccggag
    15421 gagaagcgca acgggtcaaa ctggcgacgg aactggcgaa aaaagccact ggccgcaccg
    15481 tctatattct ggatgaacca acgaccggtt tgcactttgc cgacatcgag aaattgctgc
    15541 aagtgttaat gaagctgcgc aatgccggta acacagtgat cgtgatcgaa cacaatctcg
    15601 agatgatcaa atgcgcggac tggatcatcg atctcggacc cgaaggcggc gagcgcggtg
    15661 gcgagatcgt gggaacgggt ccgccggagc ggatagtgga cattccaaat agtttcacgg
    15721 gcaaatatct gcgaccgatg ctggagaaaa aatgagtcgc atacgacgca taggtctcat
    15781 aggtctgatt gcactcgtcg cgcaaccgtt acgcgcagaa atttcgccag agctggccga
    15841 ggcgagtgcg ccgctcacag acggtgtgcc ggaggttgcg gtcgcgcgcc tggaaaagtt
    15901 gctcaacaga aatttacccg atccagagtg gcacgcggtg gctgagaaac tcgctgaagc
    15961 tcatgtaggc gcgaaacagg ccgaggcggc cctggtgtta ctgtcggatt cccggttgcg
    16021 tgaacttcca tgggcaaagt tttggcgtgc ccaggctttc gccagcttgc atcgatgggc
    16081 tgacgcgctg ccgctctacg aggaactgga agcggacgaa acctcatcct tccgcaaggc
    16141 tgccacgttt ggagcggcgg aaatgttgcg cgcgttggga agaccggatg aagcgctgac
    16201 aaaattcagg agtctcactc gagacaaaga atgggctacc cgggcgcagc tccgatcagc
    16261 gaagctctac atcgataagg gagacgctat caatgcccgc cgtgtgctgg acgacatgca
    16321 gcccaaatcg gtcggggaac aaaatgatcg gcgacttttg cgcgggcgtt tggagctggt
    16381 tctgcaacgg ccggaaaaag caatgagtgt atttgaagag cttctgaaaa cacctgagaa
    16441 ggtctcccat gcaactctga ttgcggcgct tttcggcatg gtggatgccc acgtgcagct
    16501 aaaaacgccg gacgccgggg ctgatttgat cgaggagttt atcaaccggt atcccgcgga
    16561 cccggattta cctgctctct tcgcaagatt ggacgagctg tatcgcgcgg tgcgcaagcc
    16621 atcacgcagc gaactggaga aatgggtgcg caatccggaa cagccgcgac gaacgttcgc
    16681 gcgatggtat cttgcgcgat tggaaaatcg cgccggccgc cgggatcgag cccggcaact
    16741 gtttcgcgat gtgcgacgcg ccgacgtaaa accgcctgaa gtggcagccg ccttattgga
    16801 gttcgcgcaa ctcgaagtgg aggactggaa tttcgatgag gcgattgcaa tcctggatga
    16861 tgcgcgcctg ctgcatccag agccgtccgt ggtagaccgg atcaacctgt tggccgcgaa
    16921 aacgcaattt ttggcgaaaa gattcgaggt ggcgaccgcc ggcttcgaac aaatcggcca
    16981 ttccaattca ccatgggcga agattgcgct gcttaacgcg tcagccggct ggctgcagct
    17041 cggcgatcac gagcgttttg cggccaatta caacgaactt gaaacacgag gggtcgatga
    17101 agaatcgcgc gctaatttgc gattagagga agccttgatg caagccgcca aaggcgataa
    17161 caaagcggct aaagcgctcc agcaatttat tcgcgagttc ccgaagagtc ctcgtgtctc
    17221 agaggcctgg gttagcctgg ccgagctggc ctaccattcc tcaccgccgc gcatcgacga
    17281 agcacgaaca aatctggcgc gcgcgctgga ttcgacgcca accgcggcgg caacggaacg
    17341 cgcggattat ctcatgatct ggatcgaaga ctcaaacggt gcggatgaga tacgagtgat
    17401 cgaccttgcg aagcgtttcc ttcagcaaca cggcggctcg gcgttcgctc cggaggttcg
    17461 aatgaaactg gcggagattt attatcggcg ccaggatttc gctaatgccc agacgcagtt
    17521 tgagattctg gccaaagaaa atccggataa tcgcatgggc gaaaacgctc tctttttcgc
    17581 ggccgaatcg gccatgtcca gcatgggcga gggttcgctt gatcgggcca ttgtcctgtt
    17641 cgatcaggtc gtgcggttga atggcccgct gcgatgggcg gcccgcaacg agcaagccgt
    17701 tatcgagcgc aagctgggga agccgcagga tgcgctcgtc ttatatgatg aggtgttaaa
    17761 gagcgatgcc gggctacccg aaaaacggga ggcgctttgc ggcaagggag acattttctt
    17821 tgagatgggt ggaagtcgcc cggaaaatta tcagcgagcg cttgaaattt atgaacagtt
    17881 agcgtccggc aaggacacgc cgattcactg gcgcaaccag gcgcttttta aaaaggctct
    17941 gtgcctggag aagaagaacg accggcccgg cgccctggca acgttttaca aagtactgga
    18001 ggacgaagcg cgtccagatc ggaggagcga actgttctgg tattataaag ctggatttaa
    18061 tgcggcgcgt ctattggagg atgattcgaa atgggaatct gctgctgctg tttacgaaaa
    18121 actcgccgcg gctggaggca gccggagtga ag
//
LOCUS       DASQUQ010000012         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1264722, whole genome shotgun sequence.
ACCESSION   DASQUQ010000012 DASQUQ010000000
VERSION     DASQUQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264722"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..648
                     /locus_tag="VE486_00370"
     CDS             193..648
                     /locus_tag="VE486_00370"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HZA37570.1"
                     /translation="MKKKVTGKDLTKEAPRSPRIRVGGYAILGRTIDKCRALVEGNVG
                     EYHFDCPLDNMLFGFKGVKGDDFKAQIEQGAGDQEIVEWLNQSGETKTPEEIKRWAEE
                     VEASSLYNHPEKRDFFSEEARKLGLDPAETTTFEWLEIDDRVSHAQQAA"
     gene            complement(775..>2067)
                     /locus_tag="VE486_00375"
     CDS             complement(775..>2067)
                     /locus_tag="VE486_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZA37571.1"
                     /translation="FAALCYAELASMIPVSGSAYTYSYATLGEIIAWVIGWDLILEYA
                     VGNMAVAVGWSGYFVKLCGSLFSLKFPLWLVNDHTTATAIIAQGGEALTNYSSTTLPV
                     IAGHTIAVNLPAFFVVAAVTVLLIYGIRESARTNTTIVIIKVAVVVFVIAFGAFMVHP
                     PHWQPFLPNGVGGMMSGAAIVFFAFIGFDAVSTTAEETKNPQRDMPIGIIASLIICTV
                     LYVLMAGVITGMRKYTTYFGDSAAVATAFAGRPWAQALISAGALAGLTSVLLVFQLGQ
                     PRIFMAMARDGLLPQYFAKIHPRFRTPYRTTIWTGIAVGGVAMLTDIGSLADLTNIGT
                     LFAFSLVCIGVIVLRRKDATRPRPFRVPLVPLFPLLGVFLCLALMLSLPIETWGRFVI
                     WLAIGLGIYFLYGVRHSKLRRGLDVGPTEDIPPPIIKT"
BASE COUNT          557 a          551 c          597 g          362 t
ORIGIN      
        1 atcgagatgt tcaccatgcc caataatgcg gcgcgttttt gcctttgtca tgtaaagcgt
       61 tatcaatgac gaaatccgaa tgcgaaatac gagagaaacc caaaatccga atgacaaaat
      121 aaacgctggc gtcccacgcg aactggactt cggtggcagc cgaactccgc gcttcattcg
      181 aaacagcgaa gtatgaagaa aaaagttact ggaaaagatt taacaaagga ggcgccgcga
      241 agtccgcgca ttcgcgtcgg gggttacgcg attcttggcc gcacgatcga caaatgtcgg
      301 gcactcgtgg aggggaacgt cggcgaatac catttcgatt gtccgctcga caatatgctt
      361 ttcggattca aaggcgtaaa gggcgacgat ttcaaagcgc aaatcgagca gggagcaggc
      421 gaccaggaaa tcgtcgaatg gctcaatcag agcggcgaga cgaaaacgcc ggaggaaatc
      481 aaacgctggg ccgaagaagt ggaagccagc agtctctaca accatcctga aaagcgcgat
      541 ttcttcagcg aggaggccag gaagcttggc ctcgatccgg cagaaacaac aacgttcgag
      601 tggctcgaaa tcgacgatag agtctctcac gcgcagcaag cggcgtagcg cgatcgacaa
      661 attgttgatc ctggtggacc gagcggtccc gttaaagtat ggcggcgaag ccgcatttgc
      721 ttagcatcgt ccggcggagt ggaccgatcc accacgtcac gccgatgttt gatcttatgt
      781 cttgatgatc ggcggcggga tgtcttcggt cgggccgacg tcgaggccac ggcgcagttt
      841 gctgtggcgc acgccgtaga ggaaataaat accgagcccg atcgcgagcc agataacgaa
      901 gcggccccat gtttcaattg gcagactcag catcagcgcg agacaaagga acacacccag
      961 aagcgggaat aacggaacca acggaacacg aaatgggcga ggacgggtgg cgtctttgcg
     1021 gcgcaaaaca atcacaccga tgcaaaccag actgaaggca aaaagcgttc cgatattggt
     1081 gagatcggca agagaaccga tgtcagtgag catggcaacg cctcccacag cgataccggt
     1141 ccaaatcgtg gttctataag gggtgcgaaa tcggggatga attttcgcga aatactgcgg
     1201 tagcaaaccg tcgcgcgcca tggccatgaa gatgcgcggc tgtcccaact gaaaaaccag
     1261 tagcaccgaa gtaagaccgg cgagcgcccc ggcgctgatg agcgcttgcg cccaaggcct
     1321 gccggcgaac gcagtcgcga ccgcagccga gtcgccgaaa taggtcgtgt attttctcat
     1381 tccggtaata acgcccgcca ttaagacata gagcaccgtg caaataatga ggcttgcgat
     1441 aattccgatg ggcatatcgc gctggggatt ttttgtttcc tcggcagtgg tcgagacggc
     1501 atcgaatccg ataaaggcaa aaaagacaat ggcggcgccg ctcatcatcc cgccaactcc
     1561 gttcggcaga aacggctgcc agtggggcgg gtgaaccata aaagcgccga acgcaatcac
     1621 gaaaacgact actgcgacct tgatgatcac gatcgtcgtg ttggtgcgcg cgctctcacg
     1681 aattccgtaa atcaagagaa ccgttaccgc cgccacaacg aagaacgcgg gaagattgac
     1741 tgcgatcgtg tgcccggcga tgaccggcag cgtggtcgaa gaataattcg tgagcgcctc
     1801 gccgccctgc gcgatgattg ccgtcgccgt ggtgtgatca ttcaccaacc agagcggaaa
     1861 cttcagactg aaaaggctgc cgcaaagctt cacaaaatag ccggaccagc cgaccgccac
     1921 cgccatgttg ccgacggcat attcaagaat caggtcccag ccgatgaccc acgcgatgat
     1981 ctcaccgagg gtcgcatacg aataggtgta ggctgagccg gaaacaggaa tcatcgacgc
     2041 cagctccgcg tagcaaagtg cggcgaa
//
LOCUS       DASQUQ010000013         4998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_543040, whole genome shotgun sequence.
ACCESSION   DASQUQ010000013 DASQUQ010000000
VERSION     DASQUQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543040"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..959)
                     /locus_tag="VE486_00380"
     CDS             complement(201..959)
                     /locus_tag="VE486_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37572.1"
                     /translation="MIRALLVLVTLIALGALKLPIERDLAAVHRQEHFRGVEFNLGLR
                     EKIGQLGFIAALSGFRSIVADALFMQAHVAWERTEWGRVLLLFQHVTTLQPRAILFWD
                     MAAWHMAWNASVAAMNDRTQPRLALRVKAQREYFALGKDFLERGIRNNPDRPELYEAL
                     ARLYKEKYKDHERASEFFGKAAALPGARTYNRRFSAYELSYCEGREREAYERLRQLYD
                     EGEKERLPTLISRLKFLEDKLGIPQEQRIPDKNR"
     gene            complement(956..1843)
                     /locus_tag="VE486_00385"
     CDS             complement(956..1843)
                     /locus_tag="VE486_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37573.1"
                     /translation="METRHYRAFSFRRINAITVNTLTELTRLKVFYFLLVFALLLIGS
                     SMFMVQLTFQQEFQILKDVSLGAMSIFTSLLAIVATARLIPQDIEDRTVYTILAKPVP
                     RFEYLLGKIAGVLLLLAIGTVVMGAVFFLVLYVREQTLVHAITRQMAAAPHEQVEEAL
                     RSVRASAFNIDIFPGIVIIYLKACLLAALTLFVSTFATTNIFTIVVMAFIYFIGHLQA
                     IAREYWLQAHSSGWITRVFLAIVALLFPDLQAFNLVDDIVAGTAISLSLFFKTAVLGV
                     FYTTIYTLLAAFVFYGKEL"
     gene            complement(2090..2965)
                     /locus_tag="VE486_00390"
     CDS             complement(2090..2965)
                     /locus_tag="VE486_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA37574.1"
                     /translation="MTDPAVAVRGLTKLFPVPFHRQAIVAVRDLHLRVEPGEVYGLLG
                     PNGSGKSTTLKIILGLVSPTRGRTEIFGRDSRLVESREAVGFLPENPYFYKYLTGEET
                     LRFFGRLCGLGGASLKHRMNELLDLVGLAKAQKRRLGTYSKGMLQRIGLAQALIHDPK
                     LVVLDEPTAGVDPEGSREIRDLILDLRRRGITVLLSSHLLAQAQEICDRVGILADGVL
                     VREGRLQELIAIENQTELVVAEASPPLLDEIESLIDRSNAKLIERRKSTTTLERLFLE
                     ATKSDEGRTGMTKHE"
     gene            complement(2958..3233)
                     /locus_tag="VE486_00395"
     CDS             complement(2958..3233)
                     /locus_tag="VE486_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZA37575.1"
                     /translation="MISLQVFYEGNVQGVGFRWSVRHIAKGFDVTGWVRNLADGRVEL
                     QVNGEEDEVRAFLDGIAQSELHSLIHKQIESKLEKPVTARGFEIRYD"
     gene            complement(3422..3856)
                     /locus_tag="VE486_00400"
     CDS             complement(3422..3856)
                     /locus_tag="VE486_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37576.1"
                     /translation="MKRLLLIVLPIVVLAAGLSVRAETDAEVEARKTALDLAGAFAND
                     GFKTRDGHWCSTIQPHDHALVAVNLYAGNQYWFSAGAAEPSTKVAVSVYDETGKQVQT
                     ETHNAGEKAAAGFSPANSGQYFVSIDLVEGREGSFCLVYSYK"
     gene            complement(3853..4665)
                     /locus_tag="VE486_00405"
     CDS             complement(3853..4665)
                     /locus_tag="VE486_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37577.1"
                     /translation="MIRLARLSVIGLIGGICMAVGAETGQLPADQLAKAVRSDSISIP
                     TPAEFFTALGKPGKTNWASQYRGPMPMTYRNRAQIALNLGGLIADGFIAVEAKDSQQV
                     KNIGSDIIKLAKALGVSESLLGRGSSINEFAEHNEWDTLNEELEATQNEVKSSMQSHA
                     DQDLVILVSVGGWIRGTQVVSGAIIKNYDEQSAKVLRQPALVRLMQSKVNEISPELRD
                     EPLVKDVSEQLTGIEKLVSFPPGKAPTVEEVRKVNEAVGKMMEKIENKTIAK"
     gene            4834..>4998
                     /gene="eno"
                     /locus_tag="VE486_00410"
     CDS             4834..>4998
                     /gene="eno"
                     /locus_tag="VE486_00410"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZA37578.1"
                     /translation="MAKTLVRKIIGREILDSRGNPTIEVDVRLESGALGRAAIPSGAS
                     TGEHEAWELRD"
BASE COUNT         1156 a         1411 c         1346 g         1085 t
ORIGIN      
        1 gcgttgtagc cgaccacgtc gcccagacaa acaaaatgcg tgcacttgcg ctgtcgggca
       61 tcagccagga cagcttcgag cgcctccagg ttggcgtgga cgtcactgaa aattgcgaaa
      121 cgcatttgcg tggcggttaa agattaaaaa cgttacaacg ttacaacgaa aacgatttag
      181 acaatttaac cacttaacgg ttagcgattt ttgtcgggaa ttcgctgctc ctgcgggatg
      241 ccaagtttgt cttcaagaaa tttcagccgg ctgatcaacg tcggcagccg ttccttctca
      301 ccctcgtcat agagctggcg aagccgttcg tatgcttcgc gctccctgcc ttcgcagtag
      361 gaaagctcgt aggcggagaa acgcctgttg taagtccgcg ctccgggcaa ggccgccgct
      421 ttgccgaaga attcggaagc gcgctcgtga tctttgtatt tctctttgta aagacgggca
      481 agcgcttcat aaagctccgg gcgatcggga ttatttctta tcccgcgttc gagaaaatct
      541 ttgccgagcg caaaatattc gcgctgcgct ttgacccgca acgcgagacg cggttgagtt
      601 cggtcgttca ttgcggccac gctcgcgttc catgccatat gccatgcggc catgtcccaa
      661 aacaggatcg cgcgcggctg gagggtagta acgtgctgga ataacaaaag cacccggccc
      721 cattcggtgc gctcccacgc gacatgcgcc tgcatgaaaa gagcatcagc aacaatcgat
      781 cgaaaaccgc tcagggcggc aatgaacccc agttgcccga ttttctcccg gagaccgaga
      841 ttaaattcga cgcctcggaa atgctcttgc cgatgaaccg cagccaggtc gcgctcgatt
      901 ggcaacttga gcgctccgag agcgatcaga gttaccagca cgagaagggc gcgtatcata
      961 gttccttgcc gtaaaagacg aacgctgcca gcaacgtgta gatcgttgtg taaaaaactc
     1021 ccagcacggc agtcttgaag aagaggctga gggaaatggc cgttccggcg acgatgtcat
     1081 ctaccaaatt gaacgcctgc aagtcgggaa atagcagcgc tacgattgcg agaaagacac
     1141 gcgtgatcca cccgctgctg tgcgcctgca accaatattc ccgcgcgatc gcctgcaagt
     1201 ggccgatgaa gtaaatgaaa gccatcacca caatggtgaa aatatttgtg gtggcgaagg
     1261 tggagacaaa aagcgtgagc gccgcgagta aacatgcttt caggtaaata atgacgatgc
     1321 cggggaagat atcgatgttg aatgctgaag cgcggacgga gcggagagct tcctctactt
     1381 gttcgtgcgg cgccgcggcc atctggcgcg ttatcgcgtg cacgagagtt tgctcgcgca
     1441 cgtagagcac aagaaaaaac acggcgccca tcaccactgt gccgatcgcc agcagcaaaa
     1501 gcacaccggc aatttttcca agcaaatatt cgaagcgtgg gaccggtttc gccaaaatcg
     1561 tgtaaaccgt gcgatcctcg atgtcctgcg gaatgagtcg cgcggtcgcg acaattgcga
     1621 gcaacgacgt gaagattgac atcgcgccga gcgaaacgtc tttcaggatt tgaaattcct
     1681 gctgaaaagt taattgcacc atgaacatcg agctgccgat caacagcagc gcgaaaacca
     1741 gcaggaaata aaaaactttc aggcgcgtga gctcggtcaa cgtgttgacc gtgatcgcgt
     1801 tgatgcgacg gaacgagaac gcacgataat gtcgagtttc catcagcgat ataaatgggc
     1861 aatcaatgac gaatgacgaa tgacgaaaac aaagcggaat gctcggttct ggggagcaca
     1921 ggctgccagc ctgttgtcgc cggcaccttg ccggcgaccc ttcgggcagc taccacgcgc
     1981 ttggtgaaat cgtcgctcaa aaaagaggtt ttcggtaggc tgccgaaaac aacgggctgg
     2041 cagcctgtgc tccccggatg ttcgtgctcc attattactt cgtcattcat cattcgtgct
     2101 tcgtcattcc cgtccttccc tcgtcactct tcgtcgcctc gagaaataat ctctccaggg
     2161 tcgtcgtgga tttccgacgc tcgattagtt tggcgttcga ccggtcgatc agcgattcga
     2221 tctcgtcgag gagcggaggg gaagcctcgg caacgacaag ctccgtttga ttttcaatcg
     2281 cgatcaactc ctgcaagcgg ccttcgcgca cgagcacccc atcggcgagt atgccgacgc
     2341 ggtcgcagat ttcctgcgct tgcgccagca aatgagacga aagcagcacg gtgattccac
     2401 gccgccgcaa atccagaatc aggtcgcgaa tttcgcggga gccttccgga tcgactccgg
     2461 cggtaggctc atccagcaca acgagtttcg gatcgtggat caatgcctgc gcaaggccga
     2521 tgcgctgcaa catccctttc gaataggtgc cgagccgccg cttctgcgcc ttcgccaacc
     2581 cgacgaggtc gagcaactcg ttcatccgat gctttaatga cgcccctccg agcccgcaaa
     2641 gacgcccaaa aaaacgcagg gtctcttcgc ccgtgagata tttataaaag tagggattct
     2701 ccggcagaaa accgaccgct tccctgcttt cgaccaagcg gctgtcacgg ccgaaaatct
     2761 ctgtgcgacc gcgcgttggt gaaacgaggc ccaggataat tttgagagtc gtgctcttgc
     2821 cagaaccatt cggcccaagc aatccataaa cttcgcctgg ttcaacgcgc agatggaggt
     2881 cccgcacggc aacgatcgcc tgtcgatgga atggaaccgg gaaaagtttc gtcagtcccc
     2941 ggacagcaac tgctgggtca gtcatagcga atctcaaaac cgcgggcggt aaccggcttc
     3001 tccagtttac tttcgatttg tttgtgaatc agcgagtgga gttcgctctg ggcgatccca
     3061 tcgaggaacg cgcgcacttc gtcttcctct ccattaactt gaagttccac gcggccgtcc
     3121 gcgaggttgc gtacccatcc agtgacatcg aagccttttg cgatatgccg cacggaccag
     3181 cgaaaaccga cgccctggac gttgccctcg taaaatacct gaagcgagat catggctcgt
     3241 tgattctagc aaaggagtca gcacgcgaaa gccccgttaa tacttgaacg ttgggaattg
     3301 gacgttgcgc gcctgccgcg ccgtagccgg ccgaaggcgg gttggacgtt tggaatccgg
     3361 cggagaacgc tcaacgtcca acgcaaaaac gaacacgatg acgcgcgacc aggagggcgc
     3421 gtcacttata ggagtaaacc agacagaagc tgccctcccg cccttcaacc aaatcgatcg
     3481 agacaaaata ctgaccgctg ttcgccggcg aaaagccggc ggccgccttt tccccggcat
     3541 tatgagtttc agtttggact tgcttgccgg tctcatcata aacactgacc gcgacttttg
     3601 ttgaaggctc ggcggcgcca gcggaaaacc aatattggtt ccccgcatag agattaacag
     3661 cgacgagcgc gtgatcgtgt ggctggatgg tactacacca atggccgtcc cgagttttga
     3721 acccgtcgtt tgcaaatgct ccggcaagat cgagcgcagt cttgcgcgcc tcgacctcgg
     3781 catccgtctc ggcacgcaca gaaaggcccg cggccagcac aacgatcggc agaacaatca
     3841 gtaaaaggcg tttcatttcg cgatggtctt gttctctatc ttctccatca ttttcccgac
     3901 tgcttcattc accttacgca cttcctcaac agtgggcgcc ttgcccggcg gaaaagaaac
     3961 gagcttctca atgccggtga gctgttcact tacatctttg accagcggtt cgtcgcgaag
     4021 ctcgggtgag atttcattaa ccttcgattg catcaatctg accagcgccg gttgccgcag
     4081 tactttcgcg ctttgttcgt catagttttt aataatcgcg ccgctgacta cttgcgtgcc
     4141 acgaatccag ccaccgacac tcacgagaat caccaagtct tgatcggcgt gggattgcat
     4201 cgaggatttc acttcgtttt gggtcgcttc cagttcctcg tttaacgtgt cccattcatt
     4261 gtgctcggcg aactcgttga tactgctgcc gcggccaagc aggctttcac ttactccaag
     4321 cgcttttgcc agtttgatga tgtccgatcc gatattcttg acctgctggc tgtcttttgc
     4381 ttcgacggca ataaagccat ccgcaatcaa gccgccgagg ttgagtgcga tttgcgcgcg
     4441 attccgatag gtcatcggca tcgggccgcg atattggctt gcccagttcg tcttccccgg
     4501 cttgccaagc gccgtgaaaa attcggccgg cgtcgggatg ctaatcgaat cactcctgac
     4561 ggcttttgcc agttgatcgg ccggcaactg ccccgtctcc gcgccaacgg ccatgcaaat
     4621 gcccccgatc aatccgatta cgctgagtct tgccaaacga atcatcgaac agattatccg
     4681 gtgcctcgac taaattgcca atggcaatct tgacggcgaa agagaacctc aggtcaggtt
     4741 gggttttggc cgatcacgaa gagcagttga agaatctgtt gcccagcgca tgattgcgag
     4801 gcgccatccc tttccgcgct gcgatattct acaatggcaa agaccttggt tcgtaagatt
     4861 attgggcgcg aaatcctgga ttcgcgcggc aatccaacta tcgaagtcga tgtgcgattg
     4921 gaaagcggcg cgcttggccg ggcggcgatt ccaagcggag caagcaccgg cgagcatgaa
     4981 gcgtgggaat tgcgcgat
//
LOCUS       DASQUQ010000014         4724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_812749, whole genome shotgun sequence.
ACCESSION   DASQUQ010000014 DASQUQ010000000
VERSION     DASQUQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812749"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..688)
                     /locus_tag="VE486_00415"
     CDS             complement(206..688)
                     /locus_tag="VE486_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37579.1"
                     /translation="MAAPKQNLPQLYRFCFLMLGDSRKAQEVFQTTLREAALRAAHGE
                     LPNQRFWLFRDARWRCLEASENDLQAEPLQLEECDITSAAASQIAQLEPTQLAIWISA
                     APDPQRTALALFYLDEFDCREILELAELKLSELSRLLALGRRQFQAWLDAMLPERTKP
                     "
     gene            complement(859..2100)
                     /locus_tag="VE486_00420"
     CDS             complement(859..2100)
                     /locus_tag="VE486_00420"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HZA37580.1"
                     /translation="MAYLNENYLKLQAGYLFPEVARRVREFCEANPEAAKRLIRCGIG
                     DVTEPLPRVAIDAMKRAVDELGHRETFRGYGPEQGYEFLRSAIAQHDYRDRNIEIADD
                     EIFVSDGSKCDCGYILDILGDQNKVAVPDPVYPVYVDTNVMAGHSGPARADGSCEGLL
                     YLPCTLENGFVPEPPTQHVDLVYLCFPNNPTGAVASRDQLSRWVDYAREHEALLVFDA
                     AYEAYISRLEVPRSIFEIPGARECAVELRSFSKNGGFTGVRCGFTVMPKSLLARTESG
                     QKMPLHPLWHRRWSTKANSVSYPVQRGAAALYTPEGRKQIRALIDHYMSNAKILREAV
                     AAAGMKVFGGVNAPYIWVKTPDGATSWQMFDRMLRDLNVVITPGAGFGAQGEGFFRIS
                     AFNSLENAQEVARRLETLARG"
     gene            complement(2176..2775)
                     /locus_tag="VE486_00425"
     CDS             complement(2176..2775)
                     /locus_tag="VE486_00425"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZA37581.1"
                     /translation="MAYELPKLPYAYDALEPHIDARTMEIHHTKHHQAYIDNVNKAIK
                     GKADLEKKSVEDLISDLSAVPEDIRTVVRNNGGGHANHSFFWKIMGPNAGGEPKGKLA
                     DDIKSTFGSFEEFKEKFAAAGVGRFGSGWAWLIKNKSGKLEIISTPNQDSPLMDGNTP
                     ILGVDVWEHAYYLNYQNRRPDYLKAWWNVVNWDAVGKNY"
     gene            complement(2937..>4724)
                     /gene="shc"
                     /locus_tag="VE486_00430"
     CDS             complement(2937..>4724)
                     /gene="shc"
                     /locus_tag="VE486_00430"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HZA37582.1"
                     /translation="EVDGQLQRRCVQHILKRQLLDGGWNIYHGGPSEINASVKAYFAL
                     KLAGCSADAPFMHEARANIMRLGGIPQMNTFSKLYLALLGQFPWKYLPTIPAEMALLP
                     SWMPFHIYKMSSWSRAMLMPLAIINHFKPTRVLPGEKQLHELYPIGTEQSDLRLPRSE
                     RFWTWRNFFLRVEDVLKFLHPLGFRPLRRRALEEAERWMVERIGEGSDGLAAVYPAML
                     NSLIALRALGYSKTNPVYAKAERDFAGLFVDDPEDFLIQPCLSPIWDTAINIISLVES
                     GVSPEHPALQKAADWLINKEVRIRGDWAENNSHPEASGWAFEYNNIYYPDTDDTAMVL
                     MALRLVRPNDQQSFNELFRRALGWQLSFQCDDGGWGAFDKNVTTPWLEDMPFADHNAI
                     LDPTCSDLTARTLELFGYIRFDAKAQCVRDAIKYLIDTQDDDGSWYGRWGVNYIYGTW
                     QVLRGLRAIGQDMTQDWILRGRDWLESCQNSEGGWGETCETYDNPATKGIGDSTASQT
                     AWAVMALCACGDLDRASIQRGLRFLLSSQKPDGSWDEEQITGTGFPRVFYLKYDMYRQ
                     NFPLLALATYVNYRSGLGHRPSFYRCEGS"
BASE COUNT         1062 a         1333 c         1264 g         1065 t
ORIGIN      
        1 ggaaaccatt ccgcagtctc cggcggaatg ggggtgctac gaaccaattc ggcgatcgcg
       61 cgatcaaaac tctgttgatt cgcaaatccg ttccaatcgc tcgcacgtag gacggctgag
      121 cgcatggaac ggtcgcggat gcgctcctcg ccgaccggcg cgcaattcag gagcgctcgc
      181 ttccgacgcg ataaccgcgg agtcttcatg gtttcgtcct ttcaggaagc atggcatcca
      241 gccaggcttg aaattgtcgc cgtcccagtg ccagtaaccg cgaaagctcg ctcagcttga
      301 gttccgccag ctcgagaatt tcgcggcaat cgaattcatc gagataaaaa agggcaagcg
      361 cagttctctg agggtctgga gcagcggaaa tccaaattgc caactgtgtg ggctcgagct
      421 gtgcaatttg tgacgcggcc gccgaagtaa tatcacactc ctccaactga agaggctcgg
      481 cttgcaagtc attttcgctt gcttccaaac aacgccagcg tgcgtcgcgg aaaagccaaa
      541 atcgctggtt tggcagttcc ccgtgagctg cgcgcagagc cgcttcacgc aaggtcgtct
      601 gaaaaacttc ctgtgctttg cgcgaatcgc ccagcatcaa aaaacaaaag cgataaagct
      661 gcggtagatt ttgttttgga gcggccactc tggtttttca aaacccgaac gttacgcgcc
      721 tcgactcagg ggacaaggga ggattcggaa gatagccttc caggctgtct cggccgacag
      781 catcttgctt gtcgattccc acttccgcag aaaaagagcg gcaggttgaa agcccgccgg
      841 ccgagtcagg caaggtgcct aacccctggc tagtgtttcc aagcggcgcg cgacttcctg
      901 ggcgttttcc agactgttga aagcggaaat ccggaaaaat ccttcgcctt gggcgccgaa
      961 gcccgcgccg ggcgtgatca ccacgtttaa gtcgcgcaac attcgatcga acatttgcca
     1021 gctggtcgcg ccatcgggcg ttttcaccca aatgtagggc gcgtttacgc cgccgaaaac
     1081 cttcatgccg gctgcggcaa cggcttcgcg cagaattttt gcgttgctca tgtaatgatc
     1141 aatcaatgcg cgaatctgct ttcgtccttc tggagtataa agagctgccg cgccgcgctg
     1201 cacgggataa ctcacgctgt tggccttagt gctccagcgc cgatgccaaa gcggatgcag
     1261 tggcattttt tggcctgact ccgttcgcgc aagcaatgat tttggcatga ctgtaaaccc
     1321 gcagcgcaca cccgtgaaac cgccgttctt ggaaaaactg cgcaattcta cagcgcactc
     1381 gcgcgcgccc ggaatttcga aaatcgagcg cggaacttct aaccgcgaaa tatacgcttc
     1441 ataagcagcg tcaaaaacca gcaacgcctc gtgttcccgc gcgtaatcga cccagcgtga
     1501 aagttggtca cgagaggcaa ccgctccggt ggggttattc ggaaaacaaa gataaaccag
     1561 atcgacatgc tgggtaggcg gttcgggaac aaatccgttc tcaagggtgc acggcagata
     1621 aagaagacct tcgcagctgc cgtctgctct cgctgggccg ctgtggcccg ccatcacgtt
     1681 ggtatcgaca tagaccggat agaccgggtc cgggactgcg actttgtttt ggtcgcccag
     1741 aatatcgagg atatacccgc aatcgcattt cgatccgtcc gaaacaaaaa tctcatcatc
     1801 ggcgatctcg atgttgcggt cgcgataatc gtgctgcgcg attgccgatc tcagaaactc
     1861 gtaaccttgc tcgggaccgt agccgcgaaa ggtttcacgg tgtccgagtt catccactgc
     1921 gcgcttcatc gcgtcgatcg ccacgcgcgg gagcggctcc gtgacatcgc cgattccaca
     1981 gcgaatcaaa cgtttggcag cttccggatt cgcctcgcaa aactcgcgga cacgacgcgc
     2041 aacttccgga aacaaatatc cagcctgaag cttgagataa ttttcgttta gatacgccat
     2101 ttcttctgcg atcgtcggac ggtacaaggt tagcggtcat agaccgccgc tataggaaga
     2161 atttgctggc ccgacttagt aattctttcc gaccgcgtcc cagttcacga cattccacca
     2221 agccttaaga taatcaggcc ggcgattctg ataattgaga tagtaagcgt gttcccaaac
     2281 atcgacacca aggatcggtg tgtttccatc catgagcggg ctgtcctggt tcggagtgga
     2341 aataatctca agcttcccac ttttgttttt gatgagccac gcccacccac tgccgaaacg
     2401 gcctaccccg gcagccgcaa atttctcttt gaactcttcg aagctgccga aagtggattt
     2461 gatatcatcg gcaagtttgc cttttggctc gccaccggcg tttggcccca tgattttcca
     2521 gaagaaggaa tgattggcgt ggccaccgcc attattgcgc accactgtgc gaatatcttc
     2581 cggaacggcg cttaaatcgc tgatcaaatc ctcgactgat ttcttttcca gatcggcttt
     2641 gcctttgatc gccttgttga cgttgtctat gtaagcctgg tgatgcttgg tgtgatggat
     2701 ctccatcgtg cgcgcgtcga tgtgcggctc cagagcatca taggcgtagg gtaattttgg
     2761 taattcgtaa gccataattt gtaaaattag atcgaaacgc cgccagtgac aggacttatt
     2821 tttttggcga gattggaagt caactagcct ggccgactcg gcggtcagcg ctccagcctg
     2881 accgcgcaga gaatttcagc agactggaag tctgccagcc gagtcaggct ggaagcctaa
     2941 cttccttcgc agcgataaaa actcgggcga tgtccgaggc cgcttcgata attcacgtag
     3001 gtcgccagag cgagcagggg aaaattctgg cggtacatgt cgtattttaa atagaagacg
     3061 cgcggaaacc cggtgcctgt gatttgttct tcgtcccatg aaccgtccgg cttttgcgag
     3121 ctaagcagga aacgcaagcc tcgttgaata ctcgcccgat cgagatcgcc gcaggcacag
     3181 agcgccatta ccgcccatgc cgtttgcgat gctgtgctgt cgccgatacc tttggtcgca
     3241 gggttgtcat aagtctcgca tgtctcgccc cagcccccct cgctgttctg acagctctcg
     3301 agccaatcgc gcccgcgcaa aatccagtcc tgggtcatat cctggccgat cgcgcgcagg
     3361 ccccgcagta cctgccatgt gccatagatg taattgacac cccagcgccc gtaccacgat
     3421 ccatcgtcgt cttgcgtgtc gattaaatat ttgatggcgt cgcgcacgca ctgggctttg
     3481 gcgtcgaacc tgatgtaacc gaataattca agcgtgcgtg cggttagatc actgcatgtt
     3541 ggatcgagaa tcgcattgtg atcggcaaac ggcatgtctt ccagccacgg cgtagtgacg
     3601 tttttgtcaa acgcccccca gccgccatcg tcacattgga agctgagctg ccagccaagc
     3661 gcgcggcgaa acagctcatt aaatgattgt tgatcgttcg gacgaaccag ccgcagcgcc
     3721 atcaacacca tcgccgtgtc gtcggtatcc ggataataaa tgttgttgta ctcgaatgcc
     3781 cacccgctgg cttccggatg tgaattgttt tccgcccaat caccgcggat acgcacttcc
     3841 ttgttgatca gccaatcggc ggccttttgc agcgcgggat gttccgggga aacgcctgat
     3901 tccaccagtg agatgatgtt gatggcggtg tcccaaattg gcgacagaca cggttggatg
     3961 agaaaatctt cgggatcgtc gacaaagagt ccggcgaaat ctctttctgc cttggcgtaa
     4021 accggatttg tctttgagta gccgagcgcg cgtaacgcga tcaaagagtt cagcatggcc
     4081 ggatacaccg ccgcgaggcc gtcactcccc tcgccaatcc gttcaaccat ccaccgctcc
     4141 gcttcttcaa gcgcgcgccg gcgcagcggc cgaaacccga gtggatgcag aaatttcaga
     4201 acatcttcga ctcgcagaaa gaaattgcgc caagtccaga agcgttcgct gcgggggaga
     4261 cgcaaatcgc tttgctccgt gccgatggga tagagctcgt gcaattgctt ttcgccaggc
     4321 aaaacgcgag ttggcttgaa atgattgata atggccagcg gcatcaacat ggcccggctc
     4381 caggacgaca ttttgtaaat gtgaaacggc atccaactcg gcaggagcgc catttccgca
     4441 gggatcgtcg gcaaatattt ccatggaaat tgtcccagga gcgctaggta aagcttgctg
     4501 aaagtgttca tctgaggaat accgccaagg cgcatgatgt tggcgcgtgc ttcgtgcatg
     4561 aaaggcgcat cggccgaaca gccagcgagt ttcaacgcga aatatgcctt cacggaagcg
     4621 ttaatctcgc tcgggccgcc gtgatagatg ttccagcctc cgtccagcaa ttgccgtttc
     4681 aaaatgtgtt ggacgcaacg ccgctgcaat tgcccatcga cttc
//
LOCUS       DASQUQ010000015         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_454322, whole genome shotgun sequence.
ACCESSION   DASQUQ010000015 DASQUQ010000000
VERSION     DASQUQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454322"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1019)
                     /locus_tag="VE486_00435"
     CDS             complement(84..1019)
                     /locus_tag="VE486_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HZA37583.1"
                     /translation="MKLVEIPAPDFDLAMTLDSGQVFHWEKGGSGFVGTIGDEPIYVE
                     QQGDLLKLHDRQLDCLKQSSRRGSQDGGGLLGRTVARYFALDHPLAEICASFPNDPIM
                     DAARKSCRGLRIIRQPKWECLATFICSSMKQVAHIRQISKALRERFGVPRRIDHHVAH
                     TFPSARCLAESSESELRRCALGYRAKNLLATARLVGSGEADLDAWSALSDADLRKKLC
                     ALPGVGAKVANCVMLFAYQRLRAFPIDVWIERVLRARYFPGRKKVTAQRLREFSETYF
                     GDHGGYAQQYLFHYARTALRKSGAPRDSSTAWSLP"
     gene            1177..2781
                     /locus_tag="VE486_00440"
     CDS             1177..2781
                     /locus_tag="VE486_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZA37584.1"
                     /translation="MDARLAIDSAVLLLYFVLIISIGLYMGRKEEDLKDFALGGRRIP
                     WWAVLASIIAAETSAGTFFGTPGEGFALRNFTYLQLAIGTILARILVSYIFIKPYYDY
                     KVYSIYEYLTARFGVPTKNAASAVFMITRLLASGARLYVAAIALALAYEMISGTRPGQ
                     TETLLIYIGSTIAIVVLTAIYTTLGGIKAVIWTDLIQASIMIGSALVALGLLYSAIPG
                     GWHEIVQRHGGFGLSDFLSTGLDPAKTGWEKWKGMFETEYTIFAGLIGATFITMATHG
                     TDQDMVQRMLTAPDIRRSRRSLIMSGLADIPIVLTFLSIGLLLWTYYQVHPDPTLPKT
                     PNETFCHFILYEMPVGLRGLLIAGIFATAMGSLSTALNALATSFTRDWYESYINPEST
                     NEQSLRAVRWATVWFSILMIAVASTTSYLVIVHPNVRIIPIVLGIFGYTYGSLLGVFL
                     CGMFTKRRGNNLGNVIAMIIGFIVVAILSGLPNGLANIFGGQLYIQPSWLPVMEFPWW
                     ICFGTIVTFSVAILFRTGREHHPPAA"
     gene            2880..3200
                     /locus_tag="VE486_00445"
     CDS             2880..3200
                     /locus_tag="VE486_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZA37585.1"
                     /translation="MPHVVTEKCNGCKFTDCVEVCPVACFYELDNQVVIHPEECIDCM
                     ACVDVCPVHAIYEENDLPPEFAKDIEFNATQAHQIKDSGAEAITVKKDPLPGANERKK
                     ALGY"
     gene            complement(3240..>3714)
                     /locus_tag="VE486_00450"
     CDS             complement(3240..>3714)
                     /locus_tag="VE486_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37586.1"
                     /translation="RSQQHPGLWALGDCAAIPAPDGRHYPYLAQHAMREGRHLARNVH
                     AAVRGQSPQPFVYGTLGIMGSLGHYTGFGRVMGIRLRGFVAGWVRRTYYLMVTPRWSR
                     RIRIIADWTIALLFRPDIVKIDIECERPLAARRAAAGTVSDGKHPVSSVPPAELR"
BASE COUNT          813 a         1078 c          998 g          825 t
ORIGIN      
        1 tcggaagcct atcttccgcg aagcggtacg cggatctcct ctctgtcatt ccgagcggag
       61 cccgttcgac tcgcttcgcg agctcagggt aaactccacg cagtcgagga atctcttggt
      121 gctcctgact tccgcaaagc tgtgcgcgca taatgaaaca agtattgctg cgcgtaacca
      181 ccatgatcgc caaaataggt ttccgaaaat tcgcgcagac gctgtgccgt cactttcttc
      241 cttcccggaa aatacctcgc tctcaaaacg cgctcgatcc agacatcgat cgggaaggcc
      301 cgcagccgct ggtaagcaaa tagcatgaca caattagcga cctttgcccc gacgcccggc
      361 aaagcgcaga gtttttttcg aaggtcggca tcagaaaggg ccgaccatgc atcgagatcc
      421 gcttcgcccg agccgacgag tcgcgcggtt gcgagcagat tcttcgcccg gtaaccgagc
      481 gcgcatcttc gcagttcgct ctcggagctt tcagcgaggc accgcgcgga aggaaacgta
      541 tgagcgacgt gatgatcgat cctgcgcggt acaccgaatc gttcgcgcaa cgctttcgaa
      601 atttgccgga tgtgcgccac ctgcttcatc gaagaacaaa tgaacgtggc gaggcattcc
      661 catttcggct gtcgaataat ccgcagtccg cgacaagatt ttcgcgccgc gtccatgatc
      721 ggatcattcg ggaacgacgc acagatttcc gcgagcggat gatcaagcgc gaaatagcgc
      781 gcgacggttc ttcccaacag acctccgcca tcttggctcc cacggcgact tgactgcttt
      841 aagcagtcaa gttgcctgtc gtgaagcttc aagagatcgc cttgctgttc cacatagatc
      901 ggctcgtcgc caattgttcc gacaaatcca ctgccaccct tttcccagtg aaagacctgg
      961 ccagaatcga gtgtcatcgc gagatcgaaa tctggcgcag gaatttcaac tagcttcatt
     1021 cttaccacag attaacacgg caaaaatgac gaagcacgaa tgacgaagcc agaatgaaaa
     1081 tggcttctct ttaaagtctg ccacgtttcg gacttcgtca ttcggatttc tttcgccatt
     1141 cgacattcga cattcgtcat tcaaaatagc gtccgcatgg acgcccgtct cgctatcgac
     1201 agcgcagtac ttcttttata cttcgtcctc atcatctcca tcggcctcta catgggccgg
     1261 aaggaagaag atctcaaaga cttcgctctc ggcggccgca gaatcccatg gtgggcagtg
     1321 ctcgcctcga tcattgccgc agagacgagc gccggcactt ttttcggaac gccgggggag
     1381 ggattcgcat tgcgaaactt cacctacttg caactcgcga tcggcaccat cctcgcgcgc
     1441 atcctggtca gctatatttt catcaagccc tactacgatt acaaagttta ctcgatctac
     1501 gaatacctga ctgcgcggtt tggcgtgccg acaaagaatg cggcgtcggc tgttttcatg
     1561 atcacgcgtc ttctcgcctc aggcgcgcgt ttgtatgtgg cggccatcgc gcttgctctc
     1621 gcttacgaga tgatttcggg cacgcgtccc gggcaaactg agacgctgtt gatttacatc
     1681 ggatcaacga ttgcaatcgt cgtccttact gcgatttaca cgacgctcgg cggcatcaag
     1741 gcggtaatct ggaccgactt gattcaggcc tcgattatga tcggtagcgc gctggttgcg
     1801 ctcggcctgc tttattccgc gatcccgggc ggctggcatg aaatcgtgca acgccacggt
     1861 ggattcggcc tttccgattt tctgagcaca ggcctcgatc cggcgaaaac gggttgggaa
     1921 aaatggaaag gaatgtttga aacagagtac acgatttttg cggggttgat cggcgctaca
     1981 ttcatcacca tggcgacaca cggcaccgat caggacatgg tgcagcgaat gttgaccgcg
     2041 cccgacattc gccgcagccg gcgctcgctg atcatgtctg ggctcgcgga cattccaatc
     2101 gtgctcacgt tcctcagtat tggtttgttg ctctggacgt actaccaagt gcatcccgat
     2161 ccgaccctgc caaagacgcc taacgaaaca ttttgccact ttattttgta cgaaatgccg
     2221 gtcggtctcc gcggattgtt aatcgccgga atcttcgcca ccgccatggg ttcgctcagc
     2281 accgccctta acgccctggc cacaagtttt acacgcgatt ggtacgagag ttacatcaac
     2341 ccagaatcga caaatgaaca aagtttgcgc gcggtgcgtt gggcaacggt gtggttttcc
     2401 atcctcatga tcgcggttgc ttcgacaaca tcgtatctcg taatcgtgca tcccaatgtg
     2461 cgaattatcc cgatcgtgct cggcatcttc ggttacactt acggctcgct cctcggtgtg
     2521 ttcctttgcg gaatgttcac caaaagacgc ggcaacaatc tgggcaatgt catcgccatg
     2581 atcatcggtt tcattgtggt ggccatcttg agcggattgc ccaacgggct cgcgaacatt
     2641 ttcggcgggc agctctatat ccagccgagc tggctgccgg tgatggaatt cccctggtgg
     2701 atctgtttcg gaacaatcgt cacgttttcg gtggcgattt tgttccggac tggccgcgag
     2761 catcacccgc cagcggccta acgctatata taaagtagga attgaccggc ccggcgttgc
     2821 tgggttagtt gacgcctcaa cggcagtctt tttacacaac tctaaactac ccaaatccca
     2881 tgccccacgt cgtcaccgaa aaatgtaacg gatgtaaatt caccgactgt gtggaggtgt
     2941 gcccggtcgc ctgcttctac gaactggaca accaggtagt cattcacccg gaggagtgca
     3001 ttgactgcat ggcatgtgtc gacgtgtgcc ccgtccacgc aatctacgag gagaacgacc
     3061 ttccgccgga attcgcgaaa gacattgagt tcaacgcgac ccaggctcac cagataaagg
     3121 actcgggcgc ggaggcgatc acagtgaaaa aggacccgct gcccggcgca aacgagcgaa
     3181 aaaaagccct gggttattga gtgaccggcc tgcgctcaat ctcttaggcg accgaacttt
     3241 catcgcaatt ccgcaggagg gaccgaactg acgggatgct tcccatccga gaccgtcccc
     3301 gctgcggcgc ggcgggcagc cagcgggcgt tcgcactcta tatcgatttt gacgatgtcc
     3361 ggtcggaaga gcaaggcgat tgtccagtcg gcgatgatgc ggatccgacg gctccagcgc
     3421 ggggtgacca tcaggtaata ggtgcgccgg acccacccgg cgacaaagcc gcgcaggcgg
     3481 attcccatga cgcggccaaa gccagtgtag tggcccaacg agcccatgat gccgagtgtg
     3541 ccgtaaacga atggctgagg agattgaccg cgaacggcgg cgtggacatt acgcgccaaa
     3601 tggcggccct ctcgcatcgc atgttgagca aggtagggat aatgtcttcc atcgggcgca
     3661 gggatggccg cacaatcccc gagggcccag agtccgggat gttgctgact gcgc
//
LOCUS       DASQUQ010000016         4700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_4434, whole genome shotgun sequence.
ACCESSION   DASQUQ010000016 DASQUQ010000000
VERSION     DASQUQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4434"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VE486_00455"
     CDS             complement(<1..246)
                     /locus_tag="VE486_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZA37587.1"
                     /translation="MKKLEAIIKPFKLEEVKEALAELGIEGMTVTEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKVKVEVVLADDMVEKAVSVVVAAAK"
     gene            616..4137
                     /gene="dnaE"
                     /locus_tag="VE486_00460"
     CDS             616..4137
                     /gene="dnaE"
                     /locus_tag="VE486_00460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZA37588.1"
                     /translation="MPRDSFVHLHLHTEYSLLDGAIRMKELMKQAAEFKMPAVAITDH
                     GNLFGAIEFYQEATRAGVKPIIGCEAYIAPGSHKDRPGSRRDAAYHFTLLAENETGYR
                     NLVKLVTAAHLDGFHYAPRIDKELLAAHSAGLIGLSGCLASEINSAIQANNLEKAKQS
                     AAEYRDILGAKNFFIELHDHGMEAQHKCNRVLPQIARDIGVGLVAANDVHFLRRSDHE
                     AHDVMLCIGTGKMVQDENRMRYVPELYFKSPAEMREVFRDFPEAVTNTLEIGERCHLD
                     LEFGRSKYPEYPAPAGKTRESYLRELCYQGLQRRYCERAASDAELIKRLDYELGVLER
                     TGFVSYLLIVWDFIHFAKEKGIPVGPGRGSAAGSIVAYVLGITDIDPLQYGLIFERFL
                     NPERVSPPDIDVDFCEARRAEVLEYVRQKYGERRVAQIITFGKLKAKSVVRDVGRVMG
                     LSYRDADRIAKMIPNELNITLDTAVEKNPELKRAVAAEPATRQLFDYAKVLEGLSRNA
                     GVHAAGVVIADRDLSDYIPLCRDVKGNDVISQYPMGPLNDLGLLKMDFLGLKTLTVIE
                     DTLTLIHKRDPNFSLKKIPLDDPAAFALYNRGETIGLFQMESGGMTGLSKQFDVKKLD
                     DIIALIALYRPGPMELIPEYVKAKKGITPIKYLHPLLEDICADTYGVMIYQEQVMAAA
                     SKLAGYSLAQADLLRRAMGKKDKEKMAKERENFIEGCARTNKIAERKANAIFDLLEKF
                     AGYGFNKSHSAAYGVISYQTAYLKAHFPVEFMAGLLSNEINNTDKISVFVGECKRMGI
                     SILPPDINKSGLKFTPEITVAAIRDRGAQENDAHRAPLQQNAIRYGLAAIKHVGESAM
                     EAAIRERERGGDFTSLQDFCSRLDSRIANRKMLESLIKAGAFDFLGRERAELFACVDE
                     ALSASAAAYRDRVAGQVSLFDEATAPTASRKKLITPWSGHEKLSYEKELLGFYVSGHP
                     LDEYVHVFVAKNYRPIASLGELDDRAPFKIAGAIVEVEKKFTRKEGKPFAVVWVEDLN
                     DMLEVVVWNEVYVKISDALAAGRVVEIRGTLDKREDAFRATAQEIKTLARGKTNGSNA
                     AATDTYQEPAVILQFSPATTGDELREVREILASSPGPRPVQLLFDRTDADPLRLEAGT
                     EFRVNLTHDLAARLSRWLVRTEPQRCA"
     gene            complement(4330..>4700)
                     /locus_tag="VE486_00465"
     CDS             complement(4330..>4700)
                     /locus_tag="VE486_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37589.1"
                     /translation="LDFQDTSDLIIKIAGATPIFDLTMRPIDCVGKIEIAAATMPLAP
                     AVAEVEFRGGLFQSGWVISLKERTNAQSFGALDPNGTKREFPLCSSSTNAEENILLLG
                     APARPEAQRETIRPKKRTKR"
BASE COUNT         1081 a         1254 c         1302 g         1063 t
ORIGIN      
        1 cttggccgcg gccacgacca cgctgaccgc tttctctacc atatcgtcgg cgagcacgac
       61 ttccactttc acttttggca agaaatccac ggtatattca ctgccgcgat aaatctcggt
      121 gtggcccttc tgtcggccga acccctttac ttccgtgacg gtcatccctt cgatgccaag
      181 ttcggcgagc gcctctttga cctcctcgag cttgaacggt ttgatgatcg cttccagttt
      241 tttcatgtcg atttaaactc gccgtcgcgc gctgctttgc aatcgaaagc tgagcgcagc
      301 acgcgctctc atagcaatgt tgcggcgcgg atgcaagcat cgttttttgc tctcgtgaat
      361 tagtaatccg tgaatcggaa aaggtccgcc cgcgccctca attccgtaag gcgggattgc
      421 tgaaacttga ctgcttaaag cagtcaagtt tgaaaagcat atctggcgca gaagaaatcc
      481 tctcaattcg gcgatcggat gttgagccct tgagcgcctg ctcgcgaaag ctttgggagt
      541 tggacgtttt cgtttttcgc agtcgctccc ctcgcaaatt tgccaaattc ttttgacgct
      601 taatactgga aactaatgcc ccgcgattct ttcgttcatc tgcatctgca caccgaatat
      661 tcgctactcg acggtgcgat tcgaatgaaa gagctgatga aacaggcggc cgaattcaag
      721 atgcccgccg tcgcgatcac cgatcacggc aatttatttg gcgcgattga gttttatcag
      781 gaagcgactc gcgcgggagt caaaccgatc attggttgcg aagcgtacat cgcacccggc
      841 tcgcataaag atcggccggg ctcgcggcgc gatgcggcat atcacttcac gctcctggcg
      901 gaaaacgaga ccggttatcg caaccttgtg aagctggtga cagcggcgca tctcgatgga
      961 tttcattacg cccccaggat cgacaaagag cttttggcag cgcattccgc ggggctcatt
     1021 gggctgagtg gttgtcttgc gagcgaaatc aacagcgcga ttcaggcaaa caatctcgag
     1081 aaagcgaaac aaagcgcagc cgaatatcgc gacattctcg gggcgaaaaa cttctttatc
     1141 gaactgcacg atcacggcat ggaagcgcag cacaaatgca atcgcgttct gccgcaaatt
     1201 gcgcgggata tcggcgtcgg attggtggcc gcaaatgacg ttcacttcct gcgccggagc
     1261 gatcacgaag cgcatgacgt catgctttgc atcggcaccg gcaagatggt gcaggacgaa
     1321 aaccgcatgc gttacgtgcc ggaattatat ttcaaatcgc cggccgaaat gcgcgaggtg
     1381 tttcgcgatt tccccgaagc cgttacgaac accctggaaa tcggcgaacg ttgccatctc
     1441 gatctcgaat ttggccggtc caaatatccg gagtatccgg cgcctgccgg aaagacccgc
     1501 gagagttatt tacgtgagct ttgttatcag ggcttgcaaa gacgttattg cgaacgcgcg
     1561 gccagcgacg cggagttgat caagcggctc gattatgagc tcggcgttct cgagagaacg
     1621 gggttcgtca gctatctgct cattgtttgg gatttcattc attttgccaa agagaaaggc
     1681 attcctgttg gtcccggacg cggttcggcc gcaggctcaa tagtcgctta cgtcctggga
     1741 attaccgaca tcgacccgct ccaatatgga ttgatcttcg agcgattttt gaatccggag
     1801 cgcgtttcgc cgccggatat cgatgtcgat ttttgcgaag cgcgccgcgc cgaagtcctt
     1861 gaatatgtgc ggcaaaaata tggcgaacgt cgcgttgccc aaatcatcac gtttggcaaa
     1921 ctcaaagcca agagcgtggt gcgcgatgtc ggtcgcgtga tgggtctgag ctatcgcgat
     1981 gccgatcgga tcgcgaagat gatcccaaac gagctgaata tcacgctcga tacagcggtg
     2041 gagaaaaatc ccgaattgaa acgagcggtc gcggccgagc ccgcaactcg ccagcttttt
     2101 gattacgcga aagtgctcga gggtttatcg cgcaatgccg gtgtgcatgc cgcgggcgtc
     2161 gtgatcgccg atcgggacct gtctgattac atcccgctct gccgtgacgt aaaaggcaac
     2221 gacgttatca gccagtatcc gatgggaccg ctcaacgatc tcgggctgct gaaaatggat
     2281 tttctcggat taaaaacgct gactgtgatc gaggacacgc tgaccctcat tcacaaacgg
     2341 gacccaaatt tttcactgaa gaagattccg ctcgacgatc cagcggcgtt cgcgctctac
     2401 aaccgaggcg aaaccatcgg ccttttccag atggagtccg gcggaatgac gggtctgtcg
     2461 aagcagttcg atgtaaaaaa gctcgacgat attatcgcct tgatcgcgct ttatcgaccc
     2521 ggcccgatgg agctcatccc cgagtacgtg aaggcgaaaa aagggatcac gccaatcaaa
     2581 tatctgcatc cgctgctcga agacatctgc gctgacactt atggcgtgat gatttaccag
     2641 gagcaggtaa tggctgcagc gagcaagctt gccggctatt cacttgcaca ggccgatctt
     2701 ctccggcgcg ccatgggcaa aaaggacaag gagaagatgg cgaaggaacg agagaacttc
     2761 atcgaaggct gcgctcgcac caacaagatt gcggaaagaa aagccaacgc cattttcgat
     2821 ttactggaaa aattcgcagg ctacggattc aacaagagtc acagtgcggc ctacggggtg
     2881 atcagttatc agaccgctta tcttaaagcg cactttccgg tcgagttcat ggccggattg
     2941 ctgagcaatg aaatcaacaa caccgataaa atttccgtct tcgtcggcga gtgcaaacga
     3001 atgggcattt cgattttgcc gccggatatt aacaagagcg ggttaaagtt tactccggaa
     3061 attactgttg cggcgatccg tgaccgcgga gctcaagaaa acgacgctca tagagcgccg
     3121 ctacagcaga acgcaatccg ttacggtctc gctgcgatca aacacgtcgg cgaaagcgca
     3181 atggaagctg cgatccgcga acgcgagcgg ggaggggact tcacttcgct gcaggatttt
     3241 tgcagccgac tggattcccg catcgccaat cgaaaaatgc tcgaaagcct gatcaaagcc
     3301 ggtgcattcg attttcttgg ccgtgagcgc gccgaacttt tcgcctgcgt tgatgaagcc
     3361 ctgagtgcat ccgcggctgc gtatcgggac cgcgttgcgg gtcaggtttc actgttcgac
     3421 gaggcaacag cccccacagc ctcgagaaaa aagctgatta ccccctggag cgggcatgaa
     3481 aagctttctt acgaaaagga gctgcttggc ttttacgtca gcggccaccc gctcgacgag
     3541 tatgtccacg ttttcgtggc aaaaaattat cgaccgattg cgtcattggg cgaactcgat
     3601 gaccgcgcgc cgttcaaaat cgccggcgcg attgtggagg tggagaaaaa gtttaccaga
     3661 aaagagggca aaccgttcgc agtcgtttgg gttgaagact tgaatgacat gctcgaggtc
     3721 gtcgtctgga atgaggttta cgtgaaaatt tcggacgccc tcgcggccgg gcgcgtcgtt
     3781 gaaataagag gaacactgga taaacgcgag gacgcttttc gcgccacggc tcaggagata
     3841 aagacgcttg cgcggggaaa gaccaatggg tcgaatgcag cggcgacgga tacataccag
     3901 gagccagctg tgattctgca attttctccc gcaacaacgg gagatgaatt gcgggaagtg
     3961 cgcgaaattc ttgcgagttc accgggcccc cgtccggttc agttgctgtt tgatcgcacg
     4021 gatgctgatc ccttgcgcct ggaagcggga acggagtttc gcgtcaatct tacccacgat
     4081 ctcgcggcaa ggctttcacg ctggctggtg aggacagaac cgcagcgatg cgcttaggta
     4141 ttcatttagc gttggagatc tgcgcgcgct gaggcaaacc gacaacatgc cggcttgggc
     4201 gagcaacatg ctcgcgcccc cttctcgcag atgagattct ggcatttcag gagcgcttct
     4261 gctcgcgctg gccggcatgc tgaggccaac cgtctcgcac cgcgcttggc ccactcccaa
     4321 ggcggctaac taccgctttg ttcttttttt tggccgaatc gtctcgcgtt gcgcttcagg
     4381 tcgcgcaggc gcgccgagca acaatatatt ttcttccgcg tttgtgcttg aagagcagag
     4441 cggaaattcc cgctttgtcc cgtttgggtc caaagcgcca aaggattgag cgttcgtccg
     4501 ctcttttaag ctgattaccc agccagattg aaagagtcct ccgcgaaatt ccacctctgc
     4561 taccgcaggc gcgagcggca ttgtggctgc tgcaatctcg attttgccga cgcaatcgat
     4621 gggtcgcatg gttagatcga aaattggcgt cgcaccggca atcttgatta tgaggtcgct
     4681 tgtatcttga aaatcgagtt
//
LOCUS       DASQUQ010000017         7203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1083167, whole genome shotgun sequence.
ACCESSION   DASQUQ010000017 DASQUQ010000000
VERSION     DASQUQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083167"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1418
                     /gene="hflX"
                     /locus_tag="VE486_00470"
     CDS             144..1418
                     /gene="hflX"
                     /locus_tag="VE486_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZA37590.1"
                     /translation="MIETRPHKTQERALLIGLEKEGVSKWDLQDSLDELRELANSAGA
                     EVVDTVTQKLQKPTAPYYIGRGKAESIKDSCQDRHVTSVIFDDELSPAQGRNLENLFA
                     RKVLDRTQLILDIFAQRARSREGRLQIELAQLQYLLPRLTRMWHHLSRQAGGIGTRGP
                     GETQLEVDRRRVQERIARLERELEAVRKTRAVQRQGRKRHQWPVAAVVGYTNAGKSTV
                     LNLLTGAALVAADKLFATLDPTTRSFVLPNKQRVLLTDTVGFLRKLPHTLIESFKATL
                     EEVSEADLLIHIVDLSHPRVDEQMEAVDAVIKELDAFGKQTLIVFNKIDNLPNRELSE
                     SYVTRFPGSVAISARTGEGVNKLVQALEDALSSWRLRSRFRIPSNESALIAEIHRVGH
                     VLELKYEGNDALIVAHVPPHLEQKLADYAVRG"
     gene            complement(1400..1891)
                     /locus_tag="VE486_00475"
     CDS             complement(1400..1891)
                     /locus_tag="VE486_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37591.1"
                     /translation="MRFRSPACALVSLRELPSPATSITDNRTLWIIAALGAQLRLTAN
                     MLDGMVALASGHASKIGKLYNELPDRVSDAAVFIGAGFAWGENIPLGAETNVPVVPCG
                     LAGAFEALPPDRKLPRPVAIKLVIGHPLEFASTANDRAGWSQIAASVESCVRALVAQP
                     RTA"
     gene            2047..2724
                     /gene="nth"
                     /locus_tag="VE486_00480"
     CDS             2047..2724
                     /gene="nth"
                     /locus_tag="VE486_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZA37592.1"
                     /translation="MKARERVAQLVEVWPQVYPGAHCELDFRNPLQLLVATMLSAQCT
                     DKRVNMVTPALFKKYRTAKDYANAPQAELENAIRSTGFYRNKAKSIRAAMRAIAEEHG
                     GKVPDTMEKLRALPGVGRKTANVVLGNAFGKNEGIVVDTHVARLSQRLGLTRHKDAEK
                     IERDLMKLVPREHWTDWSHWLIWHGRRRCYARKPDCSQCEVFRLCPSGKNFLRTGQAR
                     KPDSETD"
     gene            2744..3601
                     /locus_tag="VE486_00485"
     CDS             2744..3601
                     /locus_tag="VE486_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37593.1"
                     /translation="MNHSSVALHLNLDDAWPNEALGLPVVDARHWGPQLRFAGPPRLV
                     AEFYREYETHLAAPFLLYGSGDFHYLTALRLRRVAEPVVVVSFDNHPDWDVRPPKWGC
                     GGWVNRALELSQVRHVAVWGCGNFECWWPHQIFGNRRAERSGILEVHPWADNRPAKDR
                     KRRGAILRDNWREHYEQSIKDLGENNVYVTIDLDCLRAEEAVTNWESGRFTIADLEWA
                     LGKLRESSRIISADICGAYSSPQYARFKQRFAAEFDHPKLARPSPEEIRRINFAALEK
                     LWPLLAQPL"
     gene            complement(3663..4007)
                     /locus_tag="VE486_00490"
     CDS             complement(3663..4007)
                     /locus_tag="VE486_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZA37594.1"
                     /translation="MSFLAFFFIVASLLSFVVAQIVVKRAMEFSASHGFRNSRFISLM
                     IIGIFLMTISFFLTLGLLQRFDLSYLYPFQGLSVIMITVMAAVMLKETLTLQLTVGSL
                     LITVGIVLVSLS"
     gene            complement(4004..4408)
                     /locus_tag="VE486_00495"
     CDS             complement(4004..4408)
                     /locus_tag="VE486_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37595.1"
                     /translation="MQDRPSGFGNPWLQLALSVLCVFVSELLLKRGATDVAEPDNAWS
                     WTGVNGLLSPLVWWAILLIVASFISWLYVLRYVPLSIAFPLSRVVDILVPLGCWILLG
                     EFISTLRWCGIALVVIGLALVAKPVAQIEERL"
     gene            complement(4513..5679)
                     /locus_tag="VE486_00500"
     CDS             complement(4513..5679)
                     /locus_tag="VE486_00500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZA37596.1"
                     /translation="MPPTTTIPFPQGVAKVVTLGKLLNCNAWKWAFQNKCKDHRYYEI
                     VEETLEGGFEYHYLLLEDDSGNVRAIQPVFFLRQNLVEGVPGKIRSVIDAVRKVFPRL
                     LTMRVLMAGCAAGAGDLGVCDEKDELWVANALRASLRTYARQNNASLVVLKDFPAEYR
                     AALGTFTSTNYARIPSMPMTRLALHHFQNWEEYFSTLSKATRKDLRRKFRKTERAPKI
                     DMELVNDITPYVDEIHPLYLQVHERSPYKFETLTKEYFRAVSQRMPDRARFFIWRQSG
                     KIVAFSFCLICDDAVYDECMGLDYRVALDLHLYFYTLRDVITWALQQRLKYYYSNPLN
                     YEPKLHLDCELVPLDLYVMHTNSFFNPIFRRAIKYLGPTSHDPVLRRFPNADQL"
     gene            complement(6408..>7203)
                     /locus_tag="VE486_00505"
     CDS             complement(6408..>7203)
                     /locus_tag="VE486_00505"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZA37597.1"
                     /translation="LVVVFWISSRTKRFLFNRVLAQSGLDRALQYAIAQIVANVVLVV
                     GVLIVLENTGIHLGALTVFAGAVGVGVGFGLQNIASNFISGLVILAERPITIGDRVEV
                     AGITGQVHQIRARSTVIRTNDNIMMIVPNTKFIDSPVTNWTYGDPRVRFRIPVGVAYG
                     SDIPKVREALLAAGRENPNTLKEPAPSVFLEKFGDNSIDFQLVVWSSEMSARPSRYRS
                     DLNFAIEQKFREAGIEIAFPQRDLHIRDGVLKVQNVEEQESAKRRE"
BASE COUNT         1688 a         1995 c         1990 g         1530 t
ORIGIN      
        1 agcaacccgg cgttatgcca aaaggcaatt gacctggttc cggcggcaaa ctaattttga
       61 gccgctgaac ttatcgcttc tcagtcacaa cgaagctgtg aattggattc tgctgcgagc
      121 gcagcgctcg ttcgcgcaca gagatgattg aaacgcgtcc gcacaaaaca caggagcgcg
      181 cgctcctgat cggcctcgaa aaggaaggcg tctcgaaatg ggatttgcag gattcgctcg
      241 atgaattgcg agagctggcg aattccgccg gcgccgaagt cgtcgatacc gttacccaga
      301 aattgcaaaa gccgaccgct ccctactata tcggccgcgg caaagcggag tcgatcaaag
      361 actcctgcca ggatcgacac gtgacctcgg tcattttcga cgacgaactt tcacccgcgc
      421 agggccggaa cctggaaaac ttgttcgcgc gcaaagttct cgatcgtacg cagctgatac
      481 tcgacatttt cgcgcagcgc gcccgcagcc gggaaggccg cctgcagatc gaattggccc
      541 aactgcaata tcttttgcca cgtcttaccc ggatgtggca tcacttgtca cggcaggctg
      601 gcggtatcgg cacgcgcggc cccggtgaaa cgcaattgga agtcgatcgc cggcgcgtgc
      661 aggagcggat tgcccggctc gagcgggagc tggaagcggt acgtaaaacg cgagcggtgc
      721 agcgacaagg ccggaaacgc catcagtggc ctgtcgccgc ggttgtcggc tacaccaacg
      781 cgggcaaatc gacggtgctg aatctcctta ccggtgccgc tctcgtagcg gcggataagc
      841 tttttgcgac tctcgatccc accacccgca gtttcgtttt gcctaacaaa cagagagtcc
      901 tgcttaccga cacggttggg ttcctgcgga aacttcccca cacactgatc gaatcgttta
      961 aggcgactct cgaagaagtg agcgaagctg atctgctcat tcacattgtc gatcttagcc
     1021 atccacgcgt ggatgagcag atggaagcag tggatgctgt tatcaaagaa ctcgatgcgt
     1081 tcggcaaaca aaccctgatt gttttcaaca agatcgacaa tctgcccaat cgggaactct
     1141 ccgagtctta cgtgacgcgc tttcccggga gcgtcgcgat ctccgcgcgc actggcgaag
     1201 gtgttaacaa acttgtgcag gctctcgaag acgcgcttag ttcgtggcgg ttgcgttctc
     1261 ggttccgcat tccatcgaat gaatcggcct tgatcgccga gattcatcga gtcggtcacg
     1321 tgcttgaatt gaaatacgaa ggcaatgacg ccttgatcgt cgcccacgtt ccaccgcatc
     1381 tggaacagaa actggctgat tacgcagtcc ggggctgagc aaccaaagcc cggacacagg
     1441 attcaacgct ggccgcgatt tgcgaccaac cggcgcgatc gttggctgtt gatgcaaact
     1501 cgagcgggtg tccgatcacc agcttgattg ccacaggtcg cggaagcttt cgatccggcg
     1561 gcagcgcctc gaaagctccc gcaagaccgc acggcacgac cggcacgttt gtttccgcgc
     1621 cgagcggaat gttttcgccc caggcgaaac ctgcgccaat gaagactgca gcatcggaaa
     1681 cacggtccgg aagttcgttg taaagtttgc cgatttttga agcgtgaccg gaagcgagcg
     1741 caaccatccc atcgagcatg ttcgcagtca ggcgcaattg cgcgccgagt gcggcgatga
     1801 tccacaaggt tcgattatca gtaatcgaag tcgcgggcga gggcaattcc cgcaacgata
     1861 caagcgcaca tgccggcgat cgaaatcgca ttcggcgaaa catttcgcga agcaagccaa
     1921 accgtcgcgg cctgcgccca ctttcgattg cgggtcgcga tggggcggcg atccattgct
     1981 tccgtggcag gttcctgcat aattcgttgc tttaaatcca tagcaggccc tgagaatatt
     2041 acccagatga aagctcggga acgcgtcgcg cagctggtgg aggtttggcc gcaagtttat
     2101 cccggcgcgc attgcgaact ggattttcgt aatccgctgc aactgctggt ggcgacgatg
     2161 ctctcggccc aatgcacgga caagcgcgtc aatatggtca cgcccgcgct gttcaagaaa
     2221 tatcggacgg cgaaagatta cgcgaacgcg ccgcaggccg agctggagaa cgcgattaga
     2281 tcgaccggct tttatcgcaa caaagccaaa agcatccgcg cggccatgcg tgcgatcgcg
     2341 gaagaacatg gcgggaaggt cccagacacg atggagaaat tacgcgcctt gccaggtgtt
     2401 ggccgcaaaa ccgccaacgt tgttctcggc aatgcgttcg gaaaaaatga aggcatcgtc
     2461 gtggacacac atgtcgcgcg actctcgcag cggcttggcc tgaccaggca caaagacgca
     2521 gaaaagatcg agcgggattt gatgaagctc gtgccccgtg agcattggac ggactggagc
     2581 cactggctca tctggcacgg acgcaggcgt tgttacgcgc gcaagccaga ttgcagccag
     2641 tgcgaggttt tccggttatg tccgagtgga aagaattttt tgcgaacagg gcaggccagg
     2701 aagccggatt cggaaaccga ttgaagcggc gtaaggcttc ctgatgaatc attcctcggt
     2761 cgctctgcat ctcaatctgg atgatgcctg gcccaatgaa gcgttgggcc tgccggttgt
     2821 tgacgcgcgc cattggggac cgcaattgcg cttcgccggg ccgccgcgcc tggtcgccga
     2881 attttatcgc gaatacgaga cacatctggc ggcgccgttt ctcctttacg gctcgggaga
     2941 ctttcattat ttgacggccc ttcggctgcg ccgcgttgcc gagccggtcg ttgtcgtgtc
     3001 attcgacaat catccggatt gggatgtgcg cccgccaaaa tggggctgcg gcggctgggt
     3061 gaatcgcgca ctggaacttt ctcaggtgcg ccatgtcgcc gtttggggtt gcggcaattt
     3121 tgaatgctgg tggccccatc agattttcgg caaccgtcgc gccgaacgct caggcattct
     3181 tgaggttcat ccgtgggccg acaaccgtcc cgccaaagat cgaaaacggc gaggggccat
     3241 tctgcgcgac aattggcgcg agcactatga acaatccata aaagacctcg gcgaaaacaa
     3301 tgtttatgtg acaatcgatc tggattgtct tcgcgctgaa gaggcggtga caaactggga
     3361 gagcggccga tttacgattg ccgatcttga gtgggctctg ggaaaattgc gagaatcttc
     3421 tcgaattatt agcgccgaca tttgcggcgc ttattcatcg ccgcagtatg cgcgttttaa
     3481 gcaacgcttc gctgcggaat tcgatcaccc gaagctagcg cgtcccagtc cggaggaaat
     3541 ccgaaggatt aatttcgcag ccctggaaaa actctggccg ttattagcgc aaccgttgta
     3601 gggcaagcgc atcgcttgcc aatccctggc tccggcaacc gatcggttgc cctacaattt
     3661 atttagctta acgaaaccag cacgattccg acggtgatca gcaacgagcc gaccgtcagt
     3721 tgcaaggtca acgtctcttt cagcatcacc gccgccatca cggtaatcat gatgacgctt
     3781 aacccttgaa atggataaag gtaactaaga tcgaatcgtt gcagcaatcc gagtgttaag
     3841 aaaaacgaga tcgtcattaa aaatatgccg atgatcatga gcgagataaa gcgcgaattc
     3901 cgaaaaccgt gacttgcgga gaattccatt gctcgtttta caacgatctg agccacgaca
     3961 aagctcagca gggaagcaac gatgaagaag aaggcgagga aactcacaat cgttcctcga
     4021 tctgtgcgac cggtttggcc accaatgcca gaccaataac gaccagcgca atgccgcacc
     4081 agcggagggt tgagataaac tcgccgagca aaatccagca gccgagcggc accagaatat
     4141 cgaccactcg cgagagagga aacgcgatgg aaagcggaac gtatctgaga acgtaaagcc
     4201 agctgatgaa gctggcgacg atgagaagaa ttgcccacca caccagcggc gaaagcaaac
     4261 cgttaactcc tgtccaggac catgcgttgt cgggctccgc aacatctgtc gcgccgcgtt
     4321 tcaaaagaag ctccgaaacg aacacacaca gcacgctcaa ggcgagttgc agccacggat
     4381 ttccgaaacc ggaaggtcga tcctgcattc gcttttctaa ctcgccgtca tttcaccgca
     4441 accgaatttc gctgctatgt tggtggggcg agactctgtc gagccgacaa atgcgccagc
     4501 ctgtaaccgc catcaaagct gatcagcgtt cgggaaccgc cgcaagacgg ggtcgtgact
     4561 tgtcggcccg agatatttaa tcgcgcggcg gaatatcgga ttgaaaaatg aattggtgtg
     4621 catcacgtag aggtcgagcg gcaccagttc acagtcgaga tgcagcttcg gctcataatt
     4681 taacggattg ctgtagtaat atttcaatct ttgttgcagc gcccacgtga tgacgtcgcg
     4741 caacgtgtag aaatagagat gcagatcgag cgcaacccgg taatcgagtc ccatgcattc
     4801 atcataaact gcgtcatcgc agatcaggca aaaactgaac gcaacgattt tgccgctttg
     4861 ccgccagatg aaaaagcgtg cccggtccgg catccgttgg ctgacggcgc gaaaatattc
     4921 tttcgtcagt gtctcgaatt tgtagggcga gcgctcgtgc acttggaggt aaagcggatg
     4981 aatttcatca acgtaaggag ttatgtcgtt caccaattcc atgtcgatct tgggggcgcg
     5041 ttccgttttc cggaattttc tgcgcaaatc tttccgggtc gccttgctca gcgtgctaaa
     5101 atattcttcc cagttttgga aatggtggag agcgagccgc gtcatcggca tgctggggat
     5161 gcgcgcgtaa ttagttgaag taaaggttcc cagtgccgca cggtactctg ctggaaaatc
     5221 cttgagaaca acgagcgaag cgttgttttg ccgggcgtac gtccgaaggc tcgctcgcaa
     5281 cgcatttgcg acccacagtt catccttttc gtcgcacacg cccaggtcac ccgccccggc
     5341 cgcgcagcct gccattaaga cacgcatggt caggaggcgc gggaatactt tgcgaacagc
     5401 atcgataaca gaacgaattt tccccggcac gccttcgacg aggttttgcc gaagaaaaaa
     5461 cacaggctga attgcgcgca cgttgcccga atcgtcttca agcagcagat aatgatattc
     5521 aaaaccgccc tccagcgtct cttcgacgat ctcgtagtag cgatggtctt tgcacttatt
     5581 ttgaaacgcc catttccacg cgttgcagtt cagcagcttg ccgagtgtaa cgactttggc
     5641 cacaccctgc gggaacggga tggtggtagt aggtggcatc tagaaggaaa aagagtgacc
     5701 gaatcgtcaa agttgaagcg gaagaaactg tagaggcagg cgtgtcgcct gcaaaatcga
     5761 gagcagccga cacggctgcc actacagcag gacgggcaaa agccgaatgt ccggggttgc
     5821 tcttggaaaa cgtcgaggag tttcgatgta tcaagtatca ataatgcgga ccgaccccct
     5881 tcgcctcgcc cgctttcacg ccacgtttca tggccgagta aatgccaccg ccatttcgat
     5941 ttctttccac tttcccggct tccaacgcca ccgacgaagc gcgtcaaccg ctaatgtatc
     6001 aagctgtgcg aatcctgttg atctaacaac ggttacgctg gttacataac ctgttttcag
     6061 atccagcttc agctggaaca atccgcgtcc ttgaggtccc gggaacctgt cgcttctgtg
     6121 cggaccgtaa ggtgcgacag cgttggttat gtcgagcaac catggggggc gcttcctccc
     6181 ataatcggat gtgctatgcc gtactccgtt cgcgtccact gcgccagtgc cgttgaattt
     6241 tgtcgatgat gcgctcgatc tgcttcccgc tggaacttga aaagtatccc acgtaaaccg
     6301 actctcgcct gcgctcaatc ctggtgttga gatgaactgc gccagaccgg cgagactgat
     6361 gccgatgacc aaccgaaacc taatacggaa tgcattcatc taacgattca ctcgcgccgt
     6421 ttcgctgatt cttgctcttc gacgttctgc actttgagca caccatcgcg gatatggagg
     6481 tcgcgctggg ggaaggcgat ttcgattccg gcctcacgaa atttctgttc aatggcgaaa
     6541 ttgagatcgc tccgataacg actcggccga gcgctcattt cactgctcca gaccacaagc
     6601 tgaaaatcga tcgagttgtc gccgaatttt tcgagaaaaa cgctcggcgc cggctccttt
     6661 aaagtattgg gattttctcg cccggcggcg agcagtgcct cgcgcacttt ggggatatcg
     6721 ctgccgtaag caacgccgac tgggatgcgg aaccgcaccc gcggatctcc gtacgtccaa
     6781 ttggtcacgg gcgaatcaat gaatttcgta ttcggcacga tcatcatgat gttatcattc
     6841 gtgcgaatga ccgtgctgcg cgcgcgaatt tgatgcacct gaccggttat cccggctacc
     6901 tcgacgcgat ctccgatcgt gattggcctt tccgccaaaa taaccaggcc gctgatgaaa
     6961 ttgctggcga tgttttgcag gccgaagccg acccccacgc cgaccgcccc tgcgaaaact
     7021 gtcagtgcgc ccaggtgaat tccggtgttt tccaaaacaa tcaaaacgcc aaccaccagg
     7081 acgacattgg cgacgatttg agcaatggcg tactgaagtg cccgatccag tccgctctgg
     7141 gcgaggactc gattgaaaag aaagcgtttc gtcctcgagg agatccaaaa aacgacgacc
     7201 agg
//
LOCUS       DASQUQ010000018         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_92580, whole genome shotgun sequence.
ACCESSION   DASQUQ010000018 DASQUQ010000000
VERSION     DASQUQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92580"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="VE486_00510"
     CDS             <1..1768
                     /locus_tag="VE486_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZA37598.1"
                     /translation="IHSSFGLLHSFVIRHSSFVIFQRMNLSPDKKIAGILVPLFALRG
                     QDDLGIGDIGALREFIDWTAEIGFKVIQLLPINETGTDNSPYNAISALAIEPTTLQLG
                     PGSPEDLTDEDFDATIASFDLRKLRNDGVKYRHVKNLKRLLLEKAFTNFSARASEVRQ
                     SGFRKFCEEEAAWLHDYTFFRVLMEGNDDSPAWNRWPVRHQSIQRARIWLHNLPPDQQ
                     AAIRDRQHFFCYVQWIAHQQWREIKSYAEGRGVALMGDIPFGVSYHSADVFARPDEFV
                     LDWAGGAPPEPYFKDDAFTQKWGQNWGIPLYHWDRMRSNNFEWWRERVRAVQRIFHLF
                     RIDHVLGFYRIYAFPWRPRKNKQFLPLDWHQMLEQTGGRAPHFVPRDDDTAGNCEANK
                     RDGEEYLRVALEEAGATRVIGEDLGAVPNYVRPNLRSLGIAGFKIPQWEVRDGQVTPG
                     HKYERLSVATYATHDHKPIRALWEEAFERPTPTSEQSRFDLAKIALFGGFDPNLDKVD
                     FEKDFYPAIMEALFKSNAWIAIVMITDLLARNYRFNVPGTTGSENWTRRMQRSVAELR
                     SSGKMEARMRLIHDLLEKTGRK"
     gene            1922..2911
                     /locus_tag="VE486_00515"
     CDS             1922..2911
                     /locus_tag="VE486_00515"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF003916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZA37599.1"
                     /translation="MKSPLKVAVTGAAGQIGYSLVFRIASGEMFGPDQPLTLHLIEIP
                     NALDALNGVVMELHDCAFPLLESVVPTADLDEGFRDINWGLLVGSVPRKAGMERKDLL
                     GINGKIFIGQGQAIERNAAADVRVLVIGNPCNTNCLIAMNNAKEIPRNRWFAMTRLDE
                     NRARAQLAHKAGVEITRVTNMTIWGNHSSTQYPDFYNAKIDNRPANEVIADEKWLKEE
                     FILTVQQRGAAVLKARGLSSAGSAANAIIDAVRFLTNDTPGDDWHSVAVCSDGSYDVD
                     EALISSFPVRVQGDEWKIVQHLSINEFSRDKIDKSIAELKEERSLVSDLLAGT"
     gene            complement(3006..3896)
                     /locus_tag="VE486_00520"
     CDS             complement(3006..3896)
                     /locus_tag="VE486_00520"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZA37600.1"
                     /translation="MQPPPQSSIDLPGIKKLRSGKVREVFDLGETLLFVATDRLSAFD
                     VILPDPIPHKGAVLNQLSAFWFKRFDRIDNHFVTADFDDFPQHLQPFRDQLAGRSMIV
                     RKTKPLPVECVVRGYLAGSGWKEYQESQSVCGEKLPPGLQLASQLPGPIFTPATKSDT
                     GHDLNIDMNACRQILGNEMAERVRDLSLQIYAAGRDHAAECGIIVADTKFEFGVVDGK
                     LLLIDECLTPDSSRFWPKDQYVVGQSPPSFDKQFVRDYLETLDWDKTPPAPSLPQDVI
                     VKTSAKYVEAFERLTGQKLR"
BASE COUNT          923 a         1034 c         1117 g          876 t
ORIGIN      
        1 gattcattcg tcattcgggc ttcttcattc attcgtcatt cggcattcgt catttgtcat
       61 tttccagcga atgaacctgt cgccggacaa aaagatcgcg ggaattcttg tgccgctgtt
      121 tgcgctgcgg ggccaggacg atctgggaat cggcgatatc ggtgcgctgc gggagtttat
      181 agactggacc gccgaaattg gattcaaggt catccaactc ctgccgatca acgagaccgg
      241 aaccgacaac agcccttaca acgcgattag cgcgctggcg atcgagccca cgacgctaca
      301 gctggggcca ggctctccgg aggacttaac ggacgaagac tttgatgcaa cgattgcaag
      361 cttcgatctc agaaagctgc ggaacgacgg tgtgaaatat cgccacgtca aaaatttgaa
      421 acggctgctg cttgagaagg cattcacgaa tttctctgct cgcgccagtg aagttcgaca
      481 atcaggtttt cgaaaatttt gcgaagaaga agccgcttgg ctacacgatt acacattctt
      541 cagggttctc atggaaggga acgacgatag tcccgcttgg aatcgatggc cggtgcgcca
      601 tcaatcaatc cagagagcac ggatctggct gcacaatttg ccacccgacc agcaggcagc
      661 cataagagat cgccaacatt tcttctgtta cgtgcaatgg attgcgcatc agcagtggcg
      721 cgagatcaaa tcatacgctg aaggacgcgg cgtcgccctg atgggcgata ttccttttgg
      781 cgtcagctac cacagcgccg acgttttcgc ccggcctgat gaatttgtgc tcgattgggc
      841 tggcggcgcg cctcccgagc cgtacttcaa ggacgacgcg ttcacgcaaa aatggggaca
      901 gaactggggc attccgcttt atcattggga caggatgcgc tcgaacaatt tcgagtggtg
      961 gcgcgagcgc gtccgcgcgg tccagcgcat tttccacctt tttcggatcg atcacgtgct
     1021 cggcttttat cgcatttacg cgttcccgtg gcggccacgg aagaacaaac agtttctccc
     1081 gctcgattgg caccagatgc ttgagcaaac cggcggccgc gccccgcatt tcgtgccgcg
     1141 cgacgacgac accgccggga actgtgaggc gaacaagcgc gatggcgaag aatatctgcg
     1201 agttgctctg gaagaagccg gcgccacgcg cgtgatcggc gaagatcttg gcgctgtgcc
     1261 caattacgtg cggccaaacc tgaggtcact tggcattgcc ggattcaaga ttccgcagtg
     1321 ggaagtgcgg gatggacaag tcacgccagg gcacaagtat gaacggctct ccgtcgcaac
     1381 ctatgcgacg cacgatcaca aaccgattcg ggctttatgg gaggaagctt tcgagcgccc
     1441 aacaccgacc tcggaacagt cgagattcga tctggcgaaa atcgctctct tcggtggctt
     1501 tgatccaaac ctcgataaag tcgattttga aaaagacttt tatccggcca tcatggaagc
     1561 gcttttcaaa agcaacgcct ggattgccat cgtcatgatc accgatctgc tggcgcggaa
     1621 ctaccgcttc aacgtccccg gcacgacagg aagtgagaat tggacgcggc gaatgcaaag
     1681 gagcgtcgca gaacttcgct cgagtggaaa aatggaagca cggatgcggc tgatccacga
     1741 tctgctggag aaaacgggac ggaaataatg acgaagcacg aatgacaaat ggcgaattga
     1801 gcgcgaatgc tcgaatgacg aagcggcgat ccgtccgctt attcaggatt cggatttcac
     1861 tccttcgtca ttgatttgct gttgcgcatt gctgagcaca gcatccatgg ctaattaaat
     1921 tatgaagtct ccgcttaaag tcgctgtcac cggcgcagcc gggcagattg gttactcgct
     1981 tgtttttcgc atcgcctccg gagagatgtt cggaccggac caaccgctca ccctgcatct
     2041 gatcgagatt ccgaatgcgc tcgacgcact caatggtgtg gtgatggaat tgcatgattg
     2101 cgctttcccg ctgctcgaat cggtggtgcc aacggctgat ctcgacgagg gatttcgcga
     2161 cattaattgg gggctactgg tcggcagcgt cccgcgcaaa gcgggtatgg aacggaaaga
     2221 tttgctcggg atcaacggca agatttttat cgggcaaggt caggcaatcg aaaggaacgc
     2281 agctgctgat gtgcgcgtgc ttgtgattgg aaatccgtgc aacaccaatt gcctgattgc
     2341 gatgaacaac gcgaaagaaa tcccgcgtaa tcgctggttc gccatgaccc ggctggatga
     2401 aaaccgggcg cgcgcccagc ttgcccacaa ggccggcgtc gaaatcactc gcgtcaccaa
     2461 catgacgatc tggggaaatc attcttcgac ccagtatcct gatttttaca acgcgaagat
     2521 cgacaatcgc cccgccaatg aagtgattgc cgatgagaaa tggttgaagg aggaatttat
     2581 tctcacggtg cagcagcggg gagccgcggt ccttaaggcg cgcggccttt cgtctgccgg
     2641 ctcagcggca aacgcgatca tcgatgccgt gcgctttctc acgaatgaca cgccgggcga
     2701 tgattggcac agcgtggcag tttgctccga cggaagttat gatgtcgatg aagcgttgat
     2761 ttcgtcgttc ccggtgcgcg ttcagggtga tgaatggaaa atcgtgcaac atttgtcgat
     2821 caatgaattc agtcgggaca aaatcgataa gtcgatcgcc gagctgaaag aagagagatc
     2881 gctggtgagc gatttgctcg caggaacata gccatccctg gctgtgcggc gcgcgggcat
     2941 cttgcctgcg cttcttctcg acaggctgga agcccttcga ccccacaggc aagatgcctg
     3001 tgccactacc ttaatttttg gccagtcaat cgctcgaatg cttccacata tttggcggaa
     3061 gttttcacga tcacatcttg cggcagcgac ggcgccggcg gcgttttgtc ccaatcaaga
     3121 gtctccagat aatcgcggac gaattgttta tcaaagctgg gcggactctg accgacaacg
     3181 tattgatctt tcggccagaa ccgggatgag tcgggcgtaa ggcattcgtc gatcaggagc
     3241 aattttccat cgaccacgcc aaactcgaac ttcgtatccg cgacgataat cccgcattct
     3301 gctgcatgat cgcggcccgc ggcataaatt tgcaaactca agtcgcgcac gcgttccgcc
     3361 atttcattgc ccaaaatttg gcgacatgcg ttcatgtcga tgttgaggtc gtgcccggta
     3421 tcgctctttg tcgccggagt aaaaattggc cctggtaatt gcgaggcgag ttgaagtccc
     3481 ggcggcaatt tttcgccgca aacgctttgc gattcctgat attccttcca gccggagccg
     3541 gccagatatc cacggacgac gcattccacc ggcagcggct ttgttttgcg cacgatcatc
     3601 gagcgaccgg ccagctgatc gcggaatggt tgtaggtgtt gcgggaagtc gtcgaaatcc
     3661 gcagtgacaa agtgattgtc gattctatcg aagcgcttaa accaaaacgc cgatagctga
     3721 ttcaagaccg cgcctttgtg cggaattgga tccggcaaaa tcacatcaaa agcagaaagc
     3781 cgatcggtgg cgacgaagag aagtgtttcg ccgagatcaa aaacttcgcg cacctttccg
     3841 ctgcgcagtt tttttatccc gggaagatcg attgaggatt gaggcggagg ttgcataaac
     3901 gctagtgatt agtgagaggt gagaagtgag agatggggag gggataatta
//
LOCUS       DASQUQ010000019        12031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_272805, whole genome shotgun sequence.
ACCESSION   DASQUQ010000019 DASQUQ010000000
VERSION     DASQUQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272805"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..3713)
                     /locus_tag="VE486_00525"
     CDS             complement(312..3713)
                     /locus_tag="VE486_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZA37601.1"
                     /translation="MDGDERHDGAGNDDGDLRHPAKHRAKPVTAAAPPTVPPEAPLVQ
                     VKGSGLSGPFIRRPVMTVLLTLSVIVAGVATYHKLPVNDLPAVDYPIIQVRCSYPGAN
                     PTTMANNIATPLEKQFLEISGLDIVTSSSTQGNTELTLQFNLSKSIIDAATDVQAAIQ
                     RATGQLPIDLPSPPTFTKTNPNDQAIYLIGLLSDTLTDGDLYKYASTAVAQRIAILPG
                     VSEVNIYGVQGAIRIKADPAGLASRGLTMDDLASAIKSGTVYSGAGQFDGPHRTFVLL
                     PNGQIDQAEGYRNLIVARNQDGSPVYLRDVADVHDSVEDERLSRTFWMRGFNPPGSVV
                     VLAVSRQAGSNAVQVAKSVGALFPEIRASLPGSITLVPVFDRSQSIVDSVQDVKLTLV
                     IAFILVVMVIYVFLGRATDTLIPVVALPLSFLITFAVIASLGYSINNLTLMALTLAIG
                     FLVDDAIVFLENVVRRAEHGESILKAAYNTAGEISFTILSMTLSLAAVFIPLVFLPGL
                     LGRIFREFSVTIIVAILASGLVSLTLTPLMCARMLREHKPGHKRARMERWSGDFIKRV
                     IAGYGRVLDKFLDRAWLTVPILIGCIVALWFFFTHLPFTLLPPGDSGFIRGIFIAQEG
                     SSPAQMHAYQQEVNEKLKNDPNVAQFFTLAGLASRSSSSQGLLFCFLKPRSQRLPIEQ
                     CVLQLQRSISSVPGIAAVLRPNPVLQINVGATQQAQGQYAYTLNGIVPDDVYAAADQL
                     MTKLKDFKGFASLRSDYYHDTPNLTVDMDRQRAATYGVSTSAVQSLLRTAYSQNYVYL
                     IKQPEDQYQVILEVKDSERQHPSDLNNLYVRRNTGGTFSAGGAGGGTIATTTGAASNL
                     VPLRAVTSTKQVVGPLAVNHFNQFTSVSINFNLLPDVAIGDATKFIEESFAQVQRQFP
                     TVQATFQGEALVFRQLFQALPLLLLAAIFVMYVILGILYESYVHPITVLFPAIVPAVV
                     GGLFTLWAFGSSLSLYSVIGLFLLLGIVKKNGILVVDFALKRIDEGLGLREAIHEASL
                     ERFRPIMMTTFAALMGAVPLALGFGHDASARRPLGLVIVGGLIFSQLVTLFVTPVIYL
                     WLEWFQEHVLDKVPFLRSAHMHHEGKPVPHPAGTGDGELEPAGAP"
     gene            complement(3731..4873)
                     /locus_tag="VE486_00530"
     CDS             complement(3731..4873)
                     /locus_tag="VE486_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZA37602.1"
                     /translation="MKRARVRQTIPRWAYLLFVVLAAFVLYRIFQHRASEAKASQPSA
                     RPVLVAKVVTKDVPLYLDEIGTCAAYETVQVQAQVSGQIITRDFQDGSDVKKGDLLFT
                     IDPRPYQAVLDQAKAQAALDQATLKRQEDLRARKVISQQDYDTAVANAQKSQATAEAA
                     QVNLDYCYIKSPINGRVGLRNVDVGNLVGPSTPSLVTIQGLDPIYTDFTVSETDLPLV
                     RRYLGGSNVKVETRSPDGSTPPRTGDLYFIDTAVQPGAGTVKARAVTPNPDRAFWPSE
                     FVRVRFILEKLKDANLVPSQAVQISQNGPFIFVVKPDNTVDMRQVKPGQRQDGDMQVI
                     ENGLNAGETVVVTGQLALAPGTKVEPKPYNPPEAPNGGEVAAKASM"
     gene            complement(4870..5418)
                     /locus_tag="VE486_00535"
     CDS             complement(4870..5418)
                     /locus_tag="VE486_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZA37603.1"
                     /translation="MKSDWETFGPLLHGTARVWRQKLDERLKPMGLSQAKWRTLLHLS
                     LAQDALTQAEIAARLGVEEPSVVTLLHRLEREGWVTRRNSPHDRRCNMVLLGRRAQRV
                     IAQINATADELRYELLTGIAKADLQTCIKVLARIREKAEKNDKPQRSEALSVYPTHNG
                     QNRTRHPNPGKKALHGAATNAK"
     gene            complement(5591..7027)
                     /locus_tag="VE486_00540"
     CDS             complement(5591..7027)
                     /locus_tag="VE486_00540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZA37604.1"
                     /translation="MFLRFQALLIVLLVWAAIYLPALGSLEIKGEEGRRILPAVQMID
                     TGNYVVPKVGSESYLRKPPLINWLVAGSFKIFGQRNEWTARLPSVLCVLAVALMFITV
                     ARRSLGATGSTVAALIWLTSFGMIEKGRLIEIEALYVSLFAIALVCWLSWWEEKRSPW
                     LTWLVPWIFLGLGWLAKGPMHLFFFYAIVVALLWRARELRTIWNIPHLIGLVVMVAIF
                     AAWAIPLVKMTDGAHVAQVWSRQFSGRLTGEGFNFDRWALNIPRSLGYFLPWLVFVPL
                     LRGARFSSDREINIVHGLSWGIVIPLVIVDLIPGSLPRYTMPLLAPFAWLLASILTAE
                     TVAWPGWLGGKTFSLKDRQRAIALLGIATCACVCIYAVGIVPRLQARQKVKTIAAQIE
                     AVVPGSERLYAVDPDYQPFLFYVRRSVVYVSRVADLPDDARFFLVQPNNEGAAERAQQ
                     WLPTSPRRVLSIQDYRGRKVSVFAIDKR"
     gene            7187..8437
                     /gene="nusA"
                     /locus_tag="VE486_00545"
     CDS             7187..8437
                     /gene="nusA"
                     /locus_tag="VE486_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZA37605.1"
                     /translation="MNAEFIAMLDYLERERGIKREILLEAVSNALLSASKKSVGASRD
                     LRIDINPKTGEIRALANLMVVDHVDNPQDEIELSKARKIKPDANVGDTVEVEVTPRNF
                     GRIAAQTAKQAMMQRIRQVEKEMIYEEFKDRAGEIVSGTVRRFDRSDVILDLGKFEAI
                     MPQRERVVVEDYNVGDRLRAYVVAVENGIRGPEIIVSRSHPNFVRRLFELEVSEIADG
                     TVEIRGIAREAGYRTKIAVWSANEKVDPVGACVGMRGSRVKNIVRELNNEKVDIIRWS
                     SDPKEYILEALKPAKVKNLVFDTEKKTVQISVDEDQLSLAIGKKGQNARLTARLTGWE
                     INIGKDISATTAVEQKVAQAAQALAGALPITEEQALTLVKSGFTNLEGLRDADVQDLV
                     DILAVDEAKAREIHEAVHRKELVQ"
     gene            8492..10750
                     /gene="infB"
                     /locus_tag="VE486_00550"
     CDS             8492..10750
                     /gene="infB"
                     /locus_tag="VE486_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZA37606.1"
                     /translation="MATKTSSKTATRKPAARKTAAAQKPAAAAKPKVSTKKLEAEVGT
                     AHTKAAPPTRPPAQQPPAPKVAAQPAPAPHHEVERVSLIDKKKPRKKEEDGEAKTRRD
                     VLPPISRIRASLEAPAILPKPAAPAKTEAAPPPPKEAPSVLIDSTDALAPPAEAEVAE
                     PQKVILIKPPIIVKQLASELGLKPHQLIAELMNYNIFANINQTIEPDIAAKIAETHGF
                     VLEKERREKGAGVHKVEQVVVAPPPPVIEKEEELKPRAPIITFMGHVDHGKTTLMDAI
                     RKTRVAAAEAGGITQHIGAYTVDHNGAKITFIDTPGHAAFTAMRARGANVTDIVVLVI
                     AADDGLMPQTIEAINHAKAAPHVKIMVALNKIDLPGANIDRVKKQLQEHGLTPEDWGG
                     ETIVCPVSATKGTGIDQLLEMMTLQAEVMELKASPTATPRGTVIEAQVEAGRGPTATV
                     IVQMGTLKTGDPFICGDYSGKVKSLLDDRGQPVKKAGPSTPVRVLGFIGLPNAGDELL
                     VMESERSAKTLSDERLEAKRANKLTLPQRATLESLLEAPDGKKVLRIVLKCDAQGSLE
                     ALEGALKQIESKKIDLEIIHAAVGPISESDILLASASDAVVVGFNVKVESMAVPAAKR
                     EGVQVKLYSIIYELLDQIKEAMAGLLEPEHRETVIGHAEVKQVFQLSRGIVAGCLVTD
                     GRVARAARARVLRKRQPVYDGGISTLRRFQDDVKEVRSGMECGIKLGDFSEYQVGDVI
                     ECYQLEAIAQKL"
     gene            10819..11160
                     /gene="rbfA"
                     /locus_tag="VE486_00555"
     CDS             10819..11160
                     /gene="rbfA"
                     /locus_tag="VE486_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZA37607.1"
                     /translation="MLRINEVVKRELSGIIAREIRFEGALVSINHVDVTPDLKNAHVF
                     VSVLGSDIGESVLNKLASHRAILQAELARHVTMKYTPHLTFHLDDSIERGARVIEILQ
                     EIEIPHGEDGR"
     gene            11288..>12031
                     /locus_tag="VE486_00560"
     CDS             11288..>12031
                     /locus_tag="VE486_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZA37608.1"
                     /translation="MTTAKFSEIGRILREHQRFAVMSHVRPDGDALGSQLALALSLQQ
                     LGKEVRVWNEDGMLEKYSFLPRAELVTKPPSAPEDFDCAIALDTAIQNRLGTALAAVH
                     SKLWINIDHHLSNPGYGDVVYVDPDAPATAEIIFRLIKSQNLPFNRDIAENLYAAIST
                     DTGSFQYPKTSARTFEIAAELICAGLDVGRLNQQLYENYPRRRLELLRELLRTMRFEH
                     AGRVASVSLSLKTGAELGVLPEDNEGLIDH"
BASE COUNT         2893 a         3338 c         3401 g         2399 t
ORIGIN      
        1 tttatctctg gctcgaatgg ttccaggagc acgtgctcga caaagtgcca tttctgcgca
       61 gcgcgcacat gcatcatgag ggtaaacccg tcccgcatcc cgccggaacc ggcgacggcg
      121 agttggaacc agcaggcgtg ccctgatcat gacgatggaa aaaatgacga atgacgaatg
      181 acgaagctcg aaggaatgac gaaatccgaa cggcgatacg aagtcttcga tcaactcttt
      241 tgtcgttccg agcattcggt cttccttcga cgttcgatat tcgtcattcg tcattttttc
      301 catcgtcatg attagggcgc gccggctggt tccaactcgc cgtcgccggt tccggcggga
      361 tgcgggacgg gtttaccctc atgatgcatg tgcgcgctgc gcagaaatgg cactttgtcg
      421 agcacgtgct cctggaacca ttcgagccag agataaatga ccggggtcac aaaaagcgtg
      481 accaattgcg agaaaatcag gccgccaaca attaccagcc caagtggacg ccgcgccgag
      541 gcgtcgtgtc cgaagccaag cgcgagtggc acagcgccca tgagagcggc gaaggtggtc
      601 atcatgatcg ggcgaaagcg ctccaggcta gcttcatgaa tcgcctctcg aagccccaat
      661 ccctcgtcga ttcgtttcag cgcgaaatcg accaccagga ttccgttctt cttgacgatc
      721 ccgagcaaaa gaaacaaacc gatgacgctg taaagggaca agctcgatcc gaaagcccag
      781 agcgtaaaca agcctccaac gaccgccggc acgatcgcgg gaaaaagcac cgtgatggga
      841 tgcacatagc tttcgtagag aattcccagg atcacgtaca tgacaaagat cgcggcgagg
      901 agcagcaatg gcagtgcctg gaacagctgg cgaaagacga gcgcttcacc ctgaaaagtg
      961 gcttgcacag tgggaaattg ccgctgcacc tgggcaaagc tctcttcaat gaacttggtc
     1021 gcgtcgccga tggcaacatc tggcagaagg ttgaagttaa tgcttacgct ggtgaattga
     1081 ttgaagtgat tgactgccag cgggccgacg acctgctttg tcgaggttac cgcacgcaac
     1141 ggcacgagat tggatgcagc gccggtcgta gttgcgatcg tcccgccgcc tgcgccgccc
     1201 gcgctaaagg tgccgcccgt attcctgcga acgtaaagat tgttgagatc agacggatgc
     1261 tggcgttcgc tgtctttcac ctcaaggatt acctgatatt ggtcctcggg ttgcttgatc
     1321 aaatagacat agttttggga gtaggccgtg cgcaacaagc tttgcaccgc cgaagtagaa
     1381 acgccgtaag tggcagcgcg ctggcggtcc atatcgacgg taagattcgg cgtgtcgtga
     1441 tagtaatccg agcgcaggga ggcaaagcct ttgaaatctt tcagcttcgt cattaactga
     1501 tcggcggcgg cgtaaacgtc atcgggcaca atgccgttga gcgtgtaggc atattgtccc
     1561 tgggcctgct gggtcgcgcc gacattaatt tgcaacaccg ggttgggccg taggacggcc
     1621 gctatgcctg gaacactgct gattgatctc tgcaactgca gaacgcattg ctcgattggc
     1681 aggcgctggc ttcttggttt gagaaaacaa aagagcagcc cctgcgagga cgaactccgg
     1741 gaggcgagac cggcgagagt gaaaaactgc gcgacattcg ggtcgttctt tagcttttcg
     1801 ttgacttcct gctggtaggc gtgcatctgg gcgggcgaag aaccttcctg cgcgataaaa
     1861 atgcccctga tgaacccgct gtcccccggc ggcagcaatg tgaacggcaa atgggtgaag
     1921 aaaaaccaaa gcgcaacaat acatccgatc aggatgggga cagtcaacca ggcgcggtcg
     1981 agaaatttgt ccagcacccg gccataaccg gcaatcaccc gtttgataaa gtcgccgctc
     2041 catcgttcca tgcgggcgcg tttgtgacct ggtttgtgtt cgcgaagcat gcgcgcgcac
     2101 atgagcgggg tgagtgtcag agaaaccaga cccgaggcga gaatggccac gatgatcgta
     2161 acggaaaatt cacggaaaat tcggcctaac aacccgggca ggaacacaag cggaataaaa
     2221 accgcggcga gcgaaagagt catcgacagg atggtgaatg aaatttcgcc ggcggtgtta
     2281 taagccgcct tcaaaatgga ttcgccgtgc tccgcgcggc gcacgacatt ttccaggaag
     2341 acaatcgcgt catcgacgag gaatccgatg gccagcgtca atgccatcaa tgtcaaatta
     2401 ttgatggaat agccaagcga ggcaatcacc gcgaacgtga tcaggaatga gagcggtaat
     2461 gcgacgaccg gaatcagcgt atcggtcgcg cgaccaagaa acacatagat caccatcacc
     2521 accaggatga atgcaatcac cagcgtgagt ttcacatcct gaactgaatc aacgatgctc
     2581 tgagaccggt cgaaaactgg gacgagtgtg atcgaacccg gcaaactggc gcgaatctcg
     2641 ggaaagagcg ccccgaccga ttttgcgacc tgaacggcat ttgagcctgc ctgtcgcgag
     2701 acagccagca ccacgactga acccggtggg ttaaaaccgc gcatccaaaa tgtgcgcgaa
     2761 agccgctcgt cctccacgct atcgtgaacg tcagccacgt cgcgtagata gaccggcgaa
     2821 ccatcctgat tacgcgccac aatcagattg cgatagcctt cggcttgatc gatctgcccg
     2881 ttcggcagaa gaacaaacgt gcggtgcggc ccgtcgaatt gtccggcccc agagtaaacc
     2941 gtgcccgact taatcgcgct cgccagatcg tccatcgtca acccgcgcga agccaggccg
     3001 gctggatcgg ctttgatccg gatcgcgccc tgaacgccgt agatgtttac ttccgacacg
     3061 cccgggagga tggcaatccg ctgcgccacg gccgtggagg catatttata gagatcgcca
     3121 tccgtcagcg tatcagagag caatccgatt aaatagatcg cctgatcgtt cggatttgtt
     3181 tttgtgaagg ttggcgggct cggcaggtcg atgggcaact ggccggtcgc gcgctggatc
     3241 gccgcctgca catcggtagc ggcatcgatg atgcttttgc tgagattaaa ttggagggtg
     3301 agctccgtgt tcccctgcgt gctcgaactc gtgacgatgt cgagcccgga aatttccaga
     3361 aattgtttct ccagcggcgt ggcgatgttg ttcgccatcg tcgtcggatt cgcgccggga
     3421 taggaacacc tgacctgaat aatcggataa tcaactgcgg gcagatcgtt caccggcagc
     3481 ttatggtaag tggcgacccc ggccacgatg accgacaacg tcaagagaac agtcatcaca
     3541 ggccggcgaa taaacggacc ggacagcccc gagcctttca cttgaaccaa cggggcctct
     3601 ggcggtaccg tcggcggcgc ggcagccgtc accggtttgg cccgatgctt tgcgggatgg
     3661 cgaagatccc cgtcatcatt tcctgctccg tcgtgtcgct cgtctccgtc catatcggga
     3721 atgtgatatt ctacattgaa gctttggcgg caacctcacc gccgttgggc gcttcggggg
     3781 ggttgtatgg ttttggttcc acttttgttc ctggcgccag cgcaagttgg ccagtcacaa
     3841 cgacggtctc tcctgcatta agaccatttt caatcacttg catatcgcca tcctgacgct
     3901 gacctggttt aacctggcgc atatccacgg tgttatccgg cttcaccaca aaaataaacg
     3961 ggccgttctg gctgatttgc accgcctgtg acggcacaag attcgcatct ttcagctttt
     4021 ccaggatgaa gcgaacccgc acaaattctg acggccaaaa agcacggtct ggattcggtg
     4081 tcacggcacg tgctttgacc gtccccgcgc ctggctgaac ggcagtatcg atgaaataaa
     4141 gatcacccgt gcgcggcggc gtgctgccat ccggagagcg ggtttccact ttaacattag
     4201 atccacccag gtaacggcgc acgagcggca agtctgtttc agaaacggtg aaatccgtat
     4261 agatcggatc gaggccctgg attgtgacga gggacggcgt cgagggtcca accaggttac
     4321 caacgtcaac attgcgcaga cctactcgtc cgttgatggg cgatttgatg tagcaataat
     4381 cgaggttcac ctgggcagcc tcggccgttg cttgagactt ttgtgcgttc gccaccgcag
     4441 tatcgtagtc ctgctgcgat atgacctttc tcgcgcgcag gtcctcttgt cgcttcagcg
     4501 tcgcctggtc taaagcggct tgagcttttg cctggtcgag aacggcttga tacgggcgcg
     4561 ggtcgattgt gaaaagcaaa tcgccttttt ttacgtcgga gccatcttgg aaatcgcgcg
     4621 tgatgatctg cccgctcacc tgcgcctgca cctgaacagt ttcgtaggcc gcacacgtgc
     4681 caatttcatc caggtaaagc ggcacgtcct tcgtgaccac tttcgcgaca agaacaggtc
     4741 gtgcggatgg ctgcgacgcc ttcgcctcac tcgccctgtg ctgaaaaatc cgatacaaaa
     4801 caaaggcggc cagcacaacg aaaagcaggt acgcccagcg aggaatagtt tgcctgacgc
     4861 gcgcccgttt catttggcgt ttgttgcggc gccgtggagc gcctttttcc ctggattcgg
     4921 atgccgagtc ctgttctggc cgttgtgggt aggataaacg gacaatgcct cgcttcgctg
     4981 gggcttatcg ttcttctccg ctttctcacg aatccgagcc aatactttga tgcacgtttg
     5041 caggtcggct ttcgcaatgc ccgtgagcaa ttcgtaacga agttcgtccg ctgtcgcgtt
     5101 gatctgagcg atcacacgct gggcgcgtcg gccgagaagc accatgttgc aacgacgatc
     5161 atgcggcgaa ttcctgcgcg tgacccagcc ttccctctcc aggcggtgta gcaaggtgac
     5221 aacgctgggc tcttccacgc ccagacgcgc tgcaatctcc gcctgagtga gcgcgtcttg
     5281 cgcgagagag aggtgcaaaa gcgtgcgcca tttcgcctgg ctcagaccca tcggcttgag
     5341 acgctcgtcc agtttttgcc gccatacccg agcagtcccg tggagcaatg ggccgaatgt
     5401 ttcccagtca gacttcatag tgaactaaat tcgttagcat gctaaccaat tccccttctc
     5461 cgcacctgtc aaacacgcga attgccgcgc gcctttgtag ccggggtcgg tgaccccggt
     5521 ttcgggttgc gcgcagcagc gggccgggat catcgatccc ggctacaagt gcccagagtt
     5581 gaaatcggct tcaccgttta tcgatggcga aaacggaaac ctttctgccc cgataatcct
     5641 ggatcgacaa cacgcgacgc ggagacgtcg gcaaccattg ttgcgctctt tccgcagcgc
     5701 cttcattatt cggttgcacc aaaaaaaagc gtgcatcgtc cggcagatcg gcgacgcggc
     5761 tcacgtaaac gaccgagcgg cgaacgtaaa agaggaacgg ttgataatcc ggatcgaccg
     5821 cgtacagccg ctccgatccg ggaacgacgg cctctatttg cgccgcgatc gttttcactt
     5881 tttgccgcgc ttgcaaacgc gggacaattc ccaccgcgta aatacaaaca cacgcacaag
     5941 tggcaatccc gagaagagca atcgcgcgct gtcgatcttt cagggagaaa gtttttccgc
     6001 ctagccatcc cggccacgcc acagtttcgg cagtcagaat cgaggcgagt aaccatgcga
     6061 atggcgcgag cagcggcatc gtgtaacgcg gcaaagatcc gggaatgaga tcgacaatca
     6121 cgagtggaat tacgattccc caactcaaac cgtggacaat gttgatctca cggtcggacg
     6181 aaaatctggc gccgcgcaac aacggcacaa aaacaagcca gggtaaaaag tagccaagac
     6241 tccgcgggat atttagtgcc cagcggtcaa aattgaaccc ttctccggtg agccgcccgg
     6301 aaaactgtcg tgaccaaacc tgagcgacat gcgctccgtc cgtcatcttc accaacggaa
     6361 tcgcccatgc agcgaagatt gcgaccataa cgacgagacc gatcagatgt gggatgttcc
     6421 aaattgtccg cagttcacgc gcgcgccaca gcagtgcgac cacgatcgcg tagaaaaaaa
     6481 agaggtgcat cggcccttta gccagccaac cgagtccgag aaaaatccag ggcaccagcc
     6541 aggtaagcca gggcgatcgc ttctcttccc accagcttag ccagcagacc aaagcaatcg
     6601 caaagagtga aacgtaaagc gcttcgatct caatcaagcg tcctttctcg atcatgccga
     6661 agctcgtcag ccagatcaga gcggcaaccg tcgatccggt cgcgccgagg ctccgacgcg
     6721 caaccgtgat aaacataagc gcaacagcca gcacgcaaag cactgacggc aggcgcgcag
     6781 tccattcgtt gcgttgcccg aaaatcttga atgacccggc gacgagccag ttgatcagcg
     6841 gcggtttgcg caaatacgac tcgctgccga ctttcggcac aacatagttg ccggtgtcga
     6901 tcatctgcac ggcgggcaaa atccggcgtc cctcttctcc tttgatctca agcgagccaa
     6961 gcgcaggaag ataaatcgcg gcccagacca gaagtacgat caacaaggct tgaaaacgca
     7021 aaaacattga atgttgagac gttggagatt gacctatcaa cgtcaaatcg aaccgaactg
     7081 cggcttgacg agaagtcttt gcgaacgtaa catcgttcta gtccgcgcgc tgatcggagt
     7141 gggaaatggt tcccactctt tttagtctgc gggctgcatg ttttttatga acgcggaatt
     7201 tattgccatg ctcgattacc tggagcggga gcgcgggatc aaacgtgaaa tcctgcttga
     7261 ggcggtatcg aacgccctgc tctctgcctc caaaaaaagc gtgggcgcct cgcgggactt
     7321 gcgcatcgat atcaatccga agacgggaga aatccgcgct ctcgccaatc tgatggtggt
     7381 ggatcacgtc gataatccac aggatgagat cgagctctcg aaagcgcgca agatcaagcc
     7441 ggatgcaaat gtcggggaca ccgttgaggt agaagtgacg ccgcgaaatt ttggacgcat
     7501 cgccgcacaa accgcgaaac aggcaatgat gcagcggatt cgccaggtcg aaaaggaaat
     7561 gatctacgaa gagtttaagg atcgcgccgg tgaaatcgtg agcggaaccg tgcgccgatt
     7621 cgatcgctcg gacgtgatcc tcgatctcgg aaagtttgag gcgatcatgc cgcagcgcga
     7681 acgggtggtc gtggaagact acaacgtggg cgaccggctc cgcgcctacg tggtcgcagt
     7741 cgaaaacggt atccgcggcc ccgagatcat cgtctcgcgc agtcacccaa attttgtgcg
     7801 tcgtcttttt gaattggaag tgagtgaaat tgccgatggc acggttgaga ttcgcgggat
     7861 cgcacgcgaa gcaggttacc gcaccaagat tgccgtctgg agcgcgaacg agaaggtcga
     7921 tcccgtgggc gcttgcgtgg gaatgcgcgg ttcacgagtc aagaacatcg tgcgcgaact
     7981 caacaacgaa aaggtggata tcatccgctg gagttccgat ccgaaggaat acattctgga
     8041 agcgctcaag ccggcgaaag tgaagaacct ggttttcgat acggaaaaaa agaccgtcca
     8101 gatttctgtc gatgaagatc agctttcgct cgcgatcggg aaaaaaggac aaaacgcgcg
     8161 gttaactgcg cgactgaccg gctgggaaat taacatcggc aaggacatat cggcgaccac
     8221 cgcggtcgag caaaaagtgg cgcaagcagc gcaggcgctc gcgggtgcgc tgcctatcac
     8281 cgaagagcag gctctcacgc tggttaaatc cggtttcacc aatctggagg gactgcgcga
     8341 tgccgatgtg caggatttgg tggatattct cgccgttgac gaggcaaagg cgcgcgagat
     8401 tcacgaggcg gtgcaccgga aggaactcgt gcagtagtat gggtccgatt taacacgctc
     8461 gggccatgag cgaaatttta gacgaacaac aatggcgacg aaaacgagca gcaagactgc
     8521 aacgcgaaaa ccagccgcgc gtaagaccgc tgctgcccag aagccagccg ccgcagccaa
     8581 accaaaggtt tcgactaaaa agctagaggc tgaggtcggc acggcacata caaaggcggc
     8641 gccaccgacc aggccgcccg ctcagcagcc gccagcccca aaagttgcgg cgcaacctgc
     8701 tccggcacca caccatgaag tcgagcgcgt atccctcatc gacaagaaaa aaccgcgcaa
     8761 gaaggaggaa gatggcgaag caaaaaccag gcgcgacgtt ttgcctccca tttcacgcat
     8821 tcgagcatcg ctcgaggcgc cagctattct tccaaaaccc gccgcgccag caaagaccga
     8881 agctgcgcca ccgccgccaa aggaagcgcc gagcgttctt atcgatagta cggatgcgct
     8941 ggcaccccct gctgaagctg aggtagcaga gccgcagaaa gtcatcctga tcaaaccgcc
     9001 aattatcgtt aagcaattgg caagcgagct cggtttgaag ccgcatcagc tcatcgcgga
     9061 gctgatgaac tataacattt tcgctaacat caaccagaca atcgaaccgg acattgcggc
     9121 caagatcgcg gaaacccatg ggtttgttct cgaaaaagag cgccgtgaaa aaggcgccgg
     9181 cgttcacaaa gtcgaacaag tggtggtcgc gcctccgccg ccggttattg aaaaggaaga
     9241 ggaactcaag cctcgcgcgc ctattatcac gttcatggga catgtcgatc acggtaagac
     9301 gacgctcatg gacgccattc gaaagacgcg tgtggcagcg gcggaggccg gcgggattac
     9361 acaacacatc ggggcctata ccgtcgatca caatggagcg aaaattacgt ttatcgacac
     9421 acccggccac gccgctttca ccgcgatgcg cgcgcgtgga gccaatgtga ccgacattgt
     9481 cgtgctcgtc atcgcagccg acgacggcct tatgccgcag acaattgaag ccatcaatca
     9541 cgccaaagcc gcgccgcatg tgaagatcat ggttgcgctc aacaagatcg atcttccggg
     9601 cgcaaatatc gacagggtga aaaaacaact ccaggagcac gggctcacgc cagaagactg
     9661 gggcggcgaa acgattgttt gcccggtctc agcaacgaaa ggtaccggga tcgatcaatt
     9721 gctcgagatg atgacattgc aagccgaagt aatggaactc aaagcatcgc ccactgccac
     9781 gccgcgcggg actgtaatcg aagcgcaagt cgaagcaggg cggggtccta ctgccacggt
     9841 gatcgtgcaa atgggaacct taaaaaccgg cgatcctttc atctgcggcg attacagcgg
     9901 caaagtgaaa tcgctcctgg acgatcgcgg gcagccggtc aagaaagccg ggccgtccac
     9961 gccggtaaga gtgctcggct ttatcggatt gccgaatgct ggcgacgaac ttctcgtcat
    10021 ggaatcagaa cgctcggcga agacattgag cgacgaacgc ctggaggcaa aacgcgcgaa
    10081 caagctgacc ctgccgcaac gtgcgacgct cgaaagtttg ctcgaagcgc ccgatgggaa
    10141 aaaggttttg cgcattgtgc tgaagtgcga cgcacaaggt tcgctcgaag cgctggaggg
    10201 ggcgttgaaa cagatcgaaa gcaagaaaat cgatcttgag atcattcacg ccgctgtcgg
    10261 accgatctcg gaatcggaca ttttgctggc aagcgcatcg gatgctgtcg tagtcggctt
    10321 caacgtgaag gtcgagagca tggccgtccc cgccgcgaaa cgcgaaggcg tgcaggtcaa
    10381 actttacagc atcatttacg aactgctcga tcagattaag gaagcaatgg cgggtttact
    10441 cgagcctgaa caccgagaaa cggtcattgg ccatgccgag gtcaaacaag tattccaatt
    10501 gagtagaggc attgtggccg gctgtcttgt tactgatggc cgggtcgccc gcgcagcgcg
    10561 tgcccgcgtg cttcgtaaac gccagccggt ttacgacggc gggatttcga cgctgcgccg
    10621 cttccaggat gatgtaaagg aagtgcgttc gggtatggaa tgcggaatta agctcggcga
    10681 tttcagtgaa tatcaagtgg gcgatgtgat cgagtgttac caacttgaag cgatcgcgca
    10741 gaaactctaa acggcggagt aatggagtac aacactccaa tactccaacc ctccattgat
    10801 tgtcccatga aacatcgaat gctgcgaatc aacgaggttg tgaaacgaga gctgagcgga
    10861 attattgccc gagaaatccg gtttgaaggc gcgctggtca gtatcaacca tgtcgacgtg
    10921 acccccgact taaaaaatgc gcatgtgttt gtgagcgtcc ttggatcgga tatcggcgag
    10981 agcgttttga ataaactggc gtctcatcgc gcaatattgc aagccgagct ggcccgccac
    11041 gtcaccatga aatatacgcc gcatctcact tttcatctcg acgattccat cgaacgcggg
    11101 gctcgcgtca ttgagattct gcaggaaatc gaaatacctc acggcgagga tggaagatag
    11161 gcatcttgcc tgtctaggcg cgcgggcttc cagcctgcgt ctttccgcca cgctgaaagc
    11221 gtgccggcca agacagcctg gaaggctatc ttccgatctg gggcgtaatc gttactaaga
    11281 tttttccatg actacagcca aattttcgga aatcggccgg atactgcgtg aacatcagcg
    11341 cttcgcggtg atgagtcatg tccgtccgga tggggacgcg ctcggcagcc agcttgctct
    11401 cgccctctcg cttcaacagc tcgggaaaga agtccgcgtc tggaatgaag atggaatgct
    11461 ggagaagtat tcctttcttc cccgcgcgga attggtaacc aagccgcctt cggccccgga
    11521 agattttgat tgtgccattg cgctcgacac cgcaattcaa aaccggctcg gaaccgcgct
    11581 ggccgctgtt cactcgaaac tctggattaa catcgaccac catctaagta atcctggtta
    11641 tggcgatgtt gtttatgtgg accctgacgc gcctgcgacg gcggagatca ttttccggct
    11701 gataaaaagt cagaacctgc cattcaatcg cgacatcgcg gagaatcttt acgctgcgat
    11761 ctccaccgac accgggtcgt ttcaatatcc aaaaaccagc gcgcgaacgt tcgagattgc
    11821 agcggagctc atttgcgccg gcctcgatgt tggtcgcctg aaccagcagc tctatgagaa
    11881 ttatccgcgc cgacggctgg agcttttacg tgaactgttg cgcaccatgc gttttgaaca
    11941 tgcaggtcgc gtcgcaagcg ttagcctcag cttgaaaacg ggcgccgagc tgggagttct
    12001 tcccgaagac aacgaaggat taatcgatca t
//
LOCUS       DASQUQ010000020         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1083348, whole genome shotgun sequence.
ACCESSION   DASQUQ010000020 DASQUQ010000000
VERSION     DASQUQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083348"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VE486_00565"
     CDS             complement(<1..744)
                     /locus_tag="VE486_00565"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HZA37609.1"
                     /translation="MKRINVFSGAFFLLVIGNGIVCAQSPSERNPDVSNKALITKSTP
                     PTAANALAIAALHKLADDYYAWRNENYPVWSSEAGLHTWDDRLTNYSPAQIAARAQHV
                     SGLLQKVRAMKTDNWPKDERIDWILFRAQLENVDFGNRVLKFERTSPQVYVDECTNGI
                     FSLLKKEYDTPRKRALAATSRLKQMPALLKQGLSNLQSPVKLYAQLAIQSARAIDPLL
                     NQSLMALDVDLAPNERDELIEARDVALTAL"
     gene            complement(868..1074)
                     /locus_tag="VE486_00570"
     CDS             complement(868..1074)
                     /locus_tag="VE486_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37610.1"
                     /translation="MDPYAKPKERKVGAQRPKIMHLPKSLEKRSRREREAEKQAVAAE
                     RRAIKKSARRHLKRQMLAELEESE"
     gene            complement(1082..2236)
                     /locus_tag="VE486_00575"
     CDS             complement(1082..2236)
                     /locus_tag="VE486_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZA37611.1"
                     /translation="MRNRSALHFVLIIGIANFFADFTYEGARGIIGPFLGSLGASAAI
                     VGFVAGLGELMGYGLRSISGYFADKSHRHWSFAFVGYAVNMLAVPALALPGRWPLAAG
                     MVVAERVGRGIRKPTVEAMLSYAGKSIGAGWVFGLNEALDQLGATIGPLLMALVLYLN
                     GGYRTGFAVLLIPALICLAILVAARLLHPRPHELEEGAGHTLATTNLTRPYWIYLATG
                     ALIAAGFADFALIGFHFHKANTVPANLIPVFYAVAMASSAIASIPLGRIFDRFGPNIS
                     LFAFLISAAAAPLVFLGSSVSALIGMILWGVGMSAQGSLFQAILTGVIPPHKRSTAFG
                     LFDTGYGIAWFLGSAAMGLLYDKSILAVAAFSVILQLAALPLLFVANKGR"
     gene            2434..3129
                     /locus_tag="VE486_00580"
     CDS             2434..3129
                     /locus_tag="VE486_00580"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HZA37612.1"
                     /translation="MKTLGMIGGVGPESTIDYYQNIIGLYRERQRDGSYPQFIINSVD
                     LKRGLDFMAANDLAGMARYLLEEIGKLARAGANFGLISANTPHIVFDDVASKSPIPLI
                     SIVEATCAAAKARKLKRLALFGTRYTMQATFYPKVFAGEGIEIVVPDAKDQDYIHEKY
                     LNELVPGKLLSETRAGLLAIVDRMKERIDIDGVILAGTELPLILRDAEYKGITFLDTT
                     KIHCQAAVTEMLA"
BASE COUNT          835 a          942 c          928 g          761 t
ORIGIN      
        1 caaagcggtc agcgcgacat cgcgtgcctc gatcaattcg tcgcgttcgt taggcgcaag
       61 atcgacatcc agcgccatca aactttgatt caacagcgga tcgattgcgc gggccgattg
      121 gatcgcgagc tgcgcgtaaa gctttaccgg actctgcaaa ttactcagac cctgcttgag
      181 caacgccggc atttgcttta aacggctcgt cgcagccaac gcccgttttc gcggcgtgtc
      241 gtattccttt ttcaagagcg agaaaatgcc attcgtacat tcatccacat aaacctgcgg
      301 acttgttcgc tcgaatttca gaacgcgatt gccgaaatcg acgttctcga gctgggctcg
      361 gaaaaggatc cagtcgatac gctcatcctt tggccagtta tcagttttca tcgcacggac
      421 tttctgaagc aatccgctca catgttgtgc gcgcgcggcg atttgtgccg gagaataatt
      481 agttagccga tcgtcccagg tgtgaagtcc cgcctcactg ctccagacgg gataattctc
      541 atttcgccac gcgtagtaat cgtctgccag tttgtgcaat gccgcgattg ctaacgcgtt
      601 tgcagctgtg ggcggggtgg atttcgtgat tagggcctta ttcgaaacat ccgggttgcg
      661 ctcggaaggc gactgcgcgc agacgatgcc gttaccgatc acgagaagaa agaaagcgcc
      721 agagaaaaca tttatccgct tcacgccacg aatgtattga ctcgtgctga atgagaaagc
      781 gcaaacgaac ttttgagaat agccagctgt agccgggatc gacttgtcac gccgtagcca
      841 tggcgaaggc ggatcatccc ggttctttca ttcactttct tccaattcgg caagcatttg
      901 tcgtttcaaa tgccgccgcg ccgatttctt gatcgcgcgg cgctccgctg cgaccgcctg
      961 cttttcggcc tcgcgttctc gccggcttcg tttctcgagc gactttggca aatgcataat
     1021 cttaggccgc tgcgcgccta cttttcgctc ttttggtttt gcgtacggat ccatcgaaag
     1081 tctagcgccc tttgtttgcg acaaacagga gaggaagtgc ggcaagttga agaatgactg
     1141 aaaacgcggc gacggccagg atcgatttgt cgtaaagcaa acccatcgct gcgctcccga
     1201 gaaaccacgc aatcccgtag ccagtatcaa acaagccaaa cgccgtgctc cgtttgtgcg
     1261 gaggaataac gccggtcaag atcgcctgaa agagcgagcc ctgcgcgctc atcccaacgc
     1321 cccacagaat cattccgatc aatgcagaga ccgatgagcc caggaaaaca agcggcgcgg
     1381 ctgcggcgga aatgagaaag gcaaacaagg aaatattcgg gccgaatcta tcaaatatgc
     1441 ggcccaaagg aatcgatgcg attgcgctgc tcgccatggc gactgcataa aacaccggaa
     1501 tcaaatttgc cggaaccgtg ttcgctttgt gaaaatgaaa tccgatcagt gcgaaatcgg
     1561 cgaatccggc ggcaatcaat gcaccggtcg ccagataaat ccaataaggc cgagtcagat
     1621 tggtcgtggc gagagtgtgc cctgcgcctt cttccagttc gtgcggacgc ggatgaagca
     1681 ggcgcgccgc gaccaagatc gcaagacaga tcaacgccgg aataagcagc acagcgaaac
     1741 cggtccgata tccaccattg agataaagaa ccagcgccat gagcaacggt ccgatcgtcg
     1801 cacccaattg atcaagcgct tcgttcagcc caaacaccca gccggcgccg atcgatttgc
     1861 cggcgtagga aagcattgct tcgacggtcg gtttgcggat tcctctgccg acacgctccg
     1921 ccaccaccat accagccgcg agcggccatc gtcccggaag cgcgagtgct ggaacggcga
     1981 gcatgttgac cgcatatccc acgaacgcga acgaccagtg ccggtgcgat ttgtccgcga
     2041 aatagcccga gatcgagcgc aatccgtaac ccattagttc gccgagaccg gcgacaaagc
     2101 caacaatcgc cgcgctcgca cctaaagacc ccagaaacgg accgatgatt ccgcgcgctc
     2161 cttcgtaggt gaaatccgcg aagaagttcg cgattccgat aatgagaaca aaatggagcg
     2221 ccgatcgatt tctcattaac cgctaatgaa cacgaatttt gaaatgccga agctcgaatg
     2281 tcgaatgacg aattaatgat gaagccggaa tgaaaaaaat gaggatgcac ccccggttgg
     2341 gcgttcgtca tttagtcatt ccttcgatat tcatcattcg gatttcgtca tttggttaat
     2401 tcgtgtttat tcgagtccat ttgtgatcgg aacatgaaga cccttggaat gatcggcggc
     2461 gtcgggccgg aaagcacgat cgattactat cagaacatta ttgggctcta tcgcgagcga
     2521 caacgtgatg ggagctatcc gcaattcatc attaacagcg ttgatctaaa gaggggactc
     2581 gatttcatgg cagccaacga tcttgctggt atggccaggt atttgctgga ggaaattggc
     2641 aaactcgcgc gggccggcgc aaacttcggc ctgatctcag caaacacgcc tcacatcgtg
     2701 ttcgacgacg ttgcctccaa gtcgcctatt ccgctgatca gtattgtcga agcaacgtgt
     2761 gcggctgcca aagcgcggaa gttgaaacgg ctcgctttgt tcgggacgcg atacaccatg
     2821 caggcgactt tttatccaaa agtctttgca ggagagggaa tcgagatcgt tgtgccggat
     2881 gctaaggatc aggattacat acacgagaaa tacctgaacg aattggtgcc gggaaaactt
     2941 ttgtcggaga cgcgcgccgg tcttcttgca atcgttgatc gcatgaaaga aaggatcgac
     3001 atcgatggcg tcattctcgc cggaacggaa ctccccctga ttctgcgcga tgcggagtac
     3061 aagggaatta catttctgga cacgacaaaa attcattgcc aggccgctgt cacggagatg
     3121 ttggcatagg gcgagttcac aaatgttgta gccaccggcc tgtggccggt cgacctcgac
     3181 gctcctttcc acaccaagga agacggccca cagggccgtg gctacagcgg ctagagtctt
     3241 atttaactcg ctctagaatg aagcatgtca ctggccgggg tcggggaccg ccggctccgg
     3301 atcgcccatg agatttttgt tagcgcggcg tgattccctc agcgagatcg acgcggaagt
     3361 tttgtttcgt ttttagagcg ccgaaactca attcggcggt gtaatcgccg gctggcagca
     3421 gccgcttcgg atcatcgcca ccatattcaa tgctcacgtc tttggt
//
LOCUS       DASQUQ010000021         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_544101, whole genome shotgun sequence.
ACCESSION   DASQUQ010000021 DASQUQ010000000
VERSION     DASQUQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544101"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..909
                     /locus_tag="VE486_00585"
     CDS             214..909
                     /locus_tag="VE486_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HZA37613.1"
                     /translation="MKPKRSRPYVAATFAMTVDGKLTTKNLSPIDFTSREDKLHLFRQ
                     RALADAVLIGHTSLKRDNVRLGLPAELQQARIKRKQTPTPLRVIVSNKGRINSRLKIF
                     QSASSPILIFSTTRMPRKTQAALGQKAALHLAESDDVDLEEMLRILRHQYNVRTVACE
                     GGPTLFRSLLERGLVDQLNLTIAPYLFGGAKAPTLTGLSKEFLPASVHCRLIQMRVVG
                     EECFLTYRIRCKK"
     gene            969..1637
                     /locus_tag="VE486_00590"
     CDS             969..1637
                     /locus_tag="VE486_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZA37614.1"
                     /translation="MGYHLIPIITMRRLAGFSTVIALFPISVLAQATVEGRVELPKSR
                     SAPVIAKRYEVVTRGGVLSTEPPLAIVYLDGLFPRPASLPTKEIAQKDLTFLTSLLPV
                     QVGTKVEFPNLDDTYHSIFSYSPAKRFDLGRYRPEERPIPSQVFDKPGLVTLRCDIHE
                     HMRALILVLNTPYFVVTNPDGRYRLSGLPSGHYTLKAWINSKTTRERPVELKSSETLH
                     VDFP"
     gene            1634..>2589
                     /locus_tag="VE486_00595"
     CDS             1634..>2589
                     /locus_tag="VE486_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37615.1"
                     /translation="MISPESQPDKPGMARFRTKLLAAMMCVVSAMTFTGLYLAQRNVT
                     ATAERDLRENFQAELSSLGKVQEFRRAALAERCSVLASRSRIHAALEDNALDLLYPSA
                     KDELRDLMEGEEPSPEQAAASLHAKFYRFLDATGAVLSPANPRDVGALNPKAEAQLAL
                     KKLPERQQIGYIREDADPGDEIVDEIVAVPIFSTETGDVISALVVGFKPFEITGNGPA
                     GMRSGIWLNGQLHLASLSKSAQAELGVEITKAVANSASTQSSFTVAVNNTPQLLFYKR
                     LNPDSLFPPAYEICIYPLTDSMAQLNRLRWQIGGAGVLLLLGG"
BASE COUNT          593 a          718 c          704 g          574 t
ORIGIN      
        1 tcgtctgctg tctccccggc gcgctggttt atctctttta caaaccgagc ggcgcagtcg
       61 cacacgtaaa actgcgcgaa atgcagaaag aagtcgcgac cctggagcac gagatcagcg
      121 aaacggaatg atgggtacag aaaacatcga acgctcaaca tccaacgccc aacgccgaat
      181 tcagaaacgt cgatgttcgg tgtttgattc ctaatgaaac cgaagcgatc gcgtccatat
      241 gttgccgcga cgttcgcaat gacggtggat ggcaaactca ccacgaaaaa tttgtcgccg
      301 atcgatttca cctcgcgcga agacaagctg cacttgttcc ggcagcgcgc tctggcggat
      361 gcggtcctga tcgggcacac gagtttgaag cgtgacaatg tgcgtctcgg cctccctgct
      421 gagctgcaac aagcgagaat caagcggaag caaactccaa cgcccctgcg cgtgatcgtc
      481 tcaaacaaag gcaggatcaa cagccgattg aaaatttttc aatccgccag ctcgcccatc
      541 ctcatttttt caacaacccg gatgccgagg aaaactcaag ctgcgctcgg gcagaaagcc
      601 gccttgcatc ttgcggaatc ggacgatgtc gatcttgaag agatgctgcg gattctgcgc
      661 caccaataca atgtccgaac ggtcgcgtgc gaaggcggtc cgacgttgtt tcgatcgctg
      721 ctggaacgcg gtctggtcga tcaactcaat ctcaccattg ctccctacct gttcggcgga
      781 gcgaaagcgc cgactttgac cgggctgagc aaggaattct tgccggccag tgtacattgc
      841 cgcttgatcc agatgcgggt tgttggcgag gagtgtttcc tcacctaccg aatcaggtgt
      901 aaaaaatgat cttggagtgc ggattatcgc gtatctcgat cagaagacgc gcaacatcgc
      961 tgaacgacgt gggttaccat cttatcccca tcatcacgat gcgcagactc gccgggttta
     1021 gcacagtcat cgcgcttttt cccatttcag ttctggcgca ggccaccgtc gagggacgcg
     1081 tcgaactgcc gaaatcgcgt tcggcgccgg taatagcgaa gcgttatgaa gtcgtcacgc
     1141 gcggtggggt tctctcgacc gaaccgccgt tggcaatagt ttatctcgat ggtcttttcc
     1201 ccagacccgc ttcgcttccg acgaaagaaa tcgcgcaaaa ggatctgacc ttccttacat
     1261 cgcttctgcc cgtgcaagtc gggacaaaag tggaattccc aaatctggac gacacctatc
     1321 acagcatttt ctcgtactcg cctgcaaaac gtttcgatct cggccgttat cgcccggagg
     1381 agcgaccgat tccgtcacag gtctttgata agccgggact ggtgacgctt cgctgcgata
     1441 ttcatgagca tatgagggcg cttattcttg ttctaaatac gccctatttt gtggtgacga
     1501 atcccgacgg acgatatcgg ctgagcgggc ttccttccgg ccactacacg ctgaaagcat
     1561 ggatcaatag caagactacg cgcgaacgcc ccgtggaatt gaagagcagc gaaacgctgc
     1621 acgttgattt cccatgatct cccccgagag ccagccagac aaacccggca tggcacgttt
     1681 ccggacaaaa ttgctggccg cgatgatgtg cgttgtttcg gcgatgacct ttaccggact
     1741 ttatcttgct cagcgcaacg tcacggcgac ggcagagcgc gatttgcggg agaactttca
     1801 agcagaactt tcctcgcttg gtaaagtgca ggaattccgc cgcgcagcgc tggctgaacg
     1861 ttgcagcgtc ctcgcgtcga gatcgcgaat ccacgccgcg ctggaggata acgctctcga
     1921 tctgctctat ccgagcgcga aagatgaatt gcgtgacttg atggaaggcg aggagccctc
     1981 gccggaacaa gcggcggctt cgcttcatgc aaagttctat cgttttctgg atgcgaccgg
     2041 tgcggtgcta tcgccggcga atccgcgaga cgttggagcg ttaaacccaa aggcggaagc
     2101 gcagctagcc ttgaagaaat tgcctgagag gcaacagatc ggttacatcc gcgaagatgc
     2161 cgaccccggg gacgagatcg tcgatgaaat agtggcggtg ccgatctttt ctacagagac
     2221 aggcgacgtg atctccgctc ttgtcgttgg ctttaagccg ttcgagataa cgggaaatgg
     2281 ccccgcggga atgagaagtg gcatttggct aaacggccaa ttgcatttgg catcgctttc
     2341 aaagtctgcc caagctgagc ttggcgtcga aataacaaaa gcggtcgcaa attcggcttc
     2401 aacccaaagc agtttcaccg tcgcagtgaa taatacgcca cagctcttgt tttataaacg
     2461 gctcaatccg gattcgctct tcccgccggc gtacgaaatc tgcatttatc cgcttactga
     2521 ttcgatggct caattgaatc ggctccgctg gcaaattggc ggcgcgggcg tgttgctttt
     2581 gctcggcgg
//
LOCUS       DASQUQ010000022         5542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_813582, whole genome shotgun sequence.
ACCESSION   DASQUQ010000022 DASQUQ010000000
VERSION     DASQUQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813582"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..2647
                     /locus_tag="VE486_00600"
     CDS             20..2647
                     /locus_tag="VE486_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZA37616.1"
                     /translation="MRTPTTYLKPPPGYRFRPPWYARPLFYIPIAIFAALAIAFAVYA
                     SILFADLKAEARTFDLNKLEQMESASVILDRKGRIFGQIYVENRETVPYDQLPRDLIN
                     AVVAVEDAKFYRHQGYDLLAIIRAALKNFTAGHVRQGASTITQQLARNSFSLKQKTFR
                     RKLLEVFLARRIEQQFGKQKIMELYLNRIYFGGGLYGAEAAARGYFGKSAREMSLTEC
                     ATLAGLIKSPNKLSPWTDRAASREARNYALDRMRDLDLISREQCAKARAEQIAVGSRQ
                     NAQGQTYAVDYIRQQVIAAVGWNRAMNEGFRIQTTIDVDLQKVAEDSLRTNLDRIEKH
                     PDYNHQTYADYAATFRKAKANGTMTSQPAPEYLQGAVIGLDNETGDILVLVGGRDFEH
                     NQYDRALQARRPAGTAMLPFVYAAAFEKGMFPGSLVEDSPLDNRVVMIGGTTGILGEW
                     GPESAENRYEGAMTARQALSKSKDGAAVRIGMNAGVDSVLQLCATAGIRSPLRPYPAT
                     FLGASEITLAELALAYTIFPNGGWRPNAPHILERIEEKDGTLVWDANRDRTRETVIKS
                     ETAYQVDSCLVDALQSGTGKAAYTEFGLKKIPAAGKTGTAYDFTDALFAGYDSNFTCA
                     VWTGFDKPQKIYRGAFGRELALPVWVDIMNAAAQQYPPREVRQPPTLNQVEICWRSGL
                     LATDKCYDAVKSESGDNVQRRTTYMELATSTQTPSEPCTVHGEPRARLVRDLPPSDLP
                     RAALAVDLSEVTPVPVKNPTLLADTDPYNSLKPTLKPEPTPEPETQTAENQKIENKPG
                     EGQAPAAKPATAVEPGSEPSTQTSESDIPIRKAIPVQPQDKGEVEIRRAQPVKPLDQE
                     SAEETLLKSATPPPSDLYQ"
     gene            2963..4015
                     /locus_tag="VE486_00605"
     CDS             2963..4015
                     /locus_tag="VE486_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37617.1"
                     /translation="MTSSSADDFRLTVLNPGGRDPEQHFPGAISPGEGEHPPVNFHGY
                     AGCTRGVFHHTPRRAIAEDTPVVLLLRGEFRASERALTELKQHGRTVAVSLKETGMHQ
                     IAQQLCDRTRLSGFMRILGQADGCIATTPEAAEIYQRARWKHDPGTVAFIPTPYPVED
                     RQWNFSVPPDEQAGIFVGTREWDVPSRNHFAALLVARQLSEATGEPVTVVNLDGYKAR
                     RLLWELKFPEEKLCLVEEEKSYPDYLRIVARHKLVLQLDRSRVPGQVAGDALLTRTVC
                     VGGDGAIERIAFPKTCGEGRSIDEIASIALDLLRNADSRAAIVAESQQLALERLSFQA
                     VRSQLANFFAQFASAR"
     gene            complement(4077..5315)
                     /locus_tag="VE486_00610"
     CDS             complement(4077..5315)
                     /locus_tag="VE486_00610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA37618.1"
                     /translation="MTKNPPPVVACYCATFLKPEMWHIYRQITALQRISPVVIAQKRE
                     NSGRFPFDKIDIVPKPALHFLRRFWFRQLRDAPWQISGAELRALLGVLEKTDARLLHI
                     YFGHIAVHLLPLIPTWSNPSIVSFHGADVMVDMHKPLYREATRQMLNAVKLVLVRSES
                     LRRAVIDLGCDEKKVQVQRTGIPLDEFPFRERNASQNGEWRFVQAGRLIEKKGLPVAL
                     RAFASFLRQYPNATLTIAGEGPLLGQLQQLARELKVDNRVSFTGFVSQEQLRDIFYAS
                     HIFLHPSETGSDGNQEGIPNSMLEAMASGLPVFATEHGGIPEAIKNGVSGVLVPEHDH
                     EELARVLLSAAEDPKFLFRIAHAGAEVVRERFDLRVQAQRLEDIYLRTIGGAIDSARS
                     MTTGCQLKGVSVKRRFMKGV"
BASE COUNT         1299 a         1556 c         1559 g         1128 t
ORIGIN      
        1 ctcctaagtt cttccacgct tgagaacgcc cacgacatat cttaaacctc cgccgggcta
       61 tcgttttcgc ccgccctggt atgcgagacc gctattttac atcccgatag caatctttgc
      121 ggcactggcc attgccttcg cggtttacgc ttcaattctt ttcgctgact tgaaggccga
      181 ggcccgaacg ttcgatctga acaagcttga acaaatggag tcggccagcg tgatcctcga
      241 tcgaaaaggc aggatattcg gccaaatcta tgtcgagaat cgcgagaccg tgccctacga
      301 tcagttaccg cgcgatctca tcaacgcggt cgtcgccgtc gaggacgcaa aattttatcg
      361 gcatcaggga tacgatttgt tggcgatcat ccgcgcggcc ttgaaaaact tcacggctgg
      421 gcacgtgcgc cagggcgcga gcacgattac ccagcaactg gcgcggaata gtttttcgct
      481 caagcaaaaa acatttcgcc gcaagctgct cgaagttttt ctggcacgcc gaatcgagca
      541 gcagttcggc aaacaaaaaa tcatggagct gtatctcaac cggatttatt tcggcggcgg
      601 cctctacggc gcggaagcag cggcgcgcgg ttacttcggc aagtctgcgc gagaaatgtc
      661 gctgacggaa tgcgccaccc tagccggtct tattaaaagc ccgaataagc tatcgccatg
      721 gaccgatcgc gccgcttcgc gcgaagcgcg caattacgcg ctcgatcgga tgcgcgatct
      781 ggacttgatt agtcgcgaac agtgcgcgaa ggcgcgcgcg gaacaaatcg ccgtcgggag
      841 ccggcagaac gctcagggac aaacctacgc agtggattac atccgccagc aggtgatcgc
      901 ggcagtcgga tggaaccggg cgatgaacga aggttttcgc attcagacaa cgatcgatgt
      961 cgatcttcag aaggttgccg aggattctct ccggacgaat ctcgaccgga ttgaaaagca
     1021 tcctgactac aatcaccaga cgtatgccga ttacgccgcc acttttcgca aggcaaaagc
     1081 aaatggaaca atgaccagcc agccagcgcc tgaatatctt cagggcgccg taatcgggct
     1141 ggataacgag acgggcgaca tcctcgtcct cgtgggcgga cgcgattttg agcacaatca
     1201 gtacgaccgc gctctgcaag cgaggcggcc ggccggaacg gcaatgctcc cgttcgtgta
     1261 tgcggcggcg tttgaaaaag gaatgttccc gggatcgctg gtggaggatt cgccgctcga
     1321 taaccgcgtg gtcatgattg gcggcacaac cgggatcctg ggcgaatggg gacccgaaag
     1381 tgcggagaat cgttacgaag gcgccatgac ggcgaggcaa gcgctgtcca aatcaaaaga
     1441 tggcgcggcc gtccgcatcg gaatgaacgc gggagtcgat tcggtcttgc agctttgcgc
     1501 gaccgcagga attcgttcgc ctctgcgtcc ctatccggcc acatttctcg gcgccagcga
     1561 gatcacgctc gccgagcttg cccttgctta caccatattt cccaacggag gctggcggcc
     1621 aaacgcgccc cacattttgg aacggatcga ggaaaaggac ggaacactgg tttgggacgc
     1681 gaaccgcgat cgcacccgag aaaccgtgat caaatctgaa accgcctatc aagtggactc
     1741 gtgtctggtc gatgcgctcc aatcaggaac cgggaaagcc gcctacacag aatttggtct
     1801 gaaaaagatt ccagcggcgg gcaaaaccgg caccgcctac gatttcaccg atgctctttt
     1861 tgccggatac gattccaatt ttacctgcgc cgtctggacg gggtttgaca agccgcaaaa
     1921 gatttatcgc ggcgcgttcg ggcgcgagct ggccctgccg gtttgggtgg acatcatgaa
     1981 cgcggccgcc cagcagtatc cgccgcgcga agttagacag ccgccgactc tgaaccaggt
     2041 cgagatttgc tggaggtccg gcttgctggc caccgacaaa tgctacgatg cggtgaaatc
     2101 ggaatccggg gacaacgttc aacggcgcac gacctacatg gagctggcga catcaacgca
     2161 gacgccgagc gaaccttgta ccgtccacgg tgaaccgcgc gctcgcctgg tgcgcgatct
     2221 tcctcccagc gatctgccgc gcgctgcgct tgccgtggat cttagcgaag tcacaccggt
     2281 gcccgtcaaa aatccaacgt tgctggcgga cacggaccca tacaattcgc ttaagccaac
     2341 cttgaaaccg gaaccgacgc cggaaccgga aacgcagacc gccgaaaacc aaaagatcga
     2401 gaacaaaccg ggcgagggcc aggcaccggc ggcgaagccc gcgaccgccg tcgagccggg
     2461 aagcgagccc agcactcaaa cgtcggagtc cgatatcccg atcaggaaag cgatcccggt
     2521 tcagcctcaa gacaaaggag aagtcgagat tcgacgtgcg caaccagtta aaccgctcga
     2581 tcaggagagc gcggaggaga ctctgttgaa atcagcaacg ccgcctccga gcgatcttta
     2641 ccaataagcg cgttcaggaa tgacgaagca cgaatgtcga atgacgaacg aatgacgagg
     2701 ctcgaagtag cggcgttctg tgagcgtcgc attattttct ttctatacgc gcgccagctg
     2761 gaataacgag gcacgactga cgaattacaa acaattgtcg gcgcttattt tgtcattccg
     2821 accgaagtgg aggaacctct tgttattaaa aagggaaaag gtaagagatg tctcccgacc
     2881 ttcgcataaa gcgtggcagg cgacttcgct ccacatgaca atggaaacct tcgaactcac
     2941 gaatgcttgg ccacgataaa caatgacttc gtcgagcgca gatgatttcc ggttgaccgt
     3001 tctcaatccg ggcgggcgcg atccagaaca gcactttcct ggcgcaatca gcccgggcga
     3061 gggtgaacat ccgcccgtta attttcacgg ttacgccggg tgcacacgcg gagtttttca
     3121 tcacactccg cggcgcgcga tcgcagaaga tacaccggtc gttctcctgt tgcgtggcga
     3181 gttccgggct tcggagcggg cattaaccga actcaagcaa catggacgga ccgtcgcggt
     3241 atcgctgaag gagacgggta tgcaccagat tgcgcaacaa ttatgcgatc gcacgagatt
     3301 atcgggcttc atgaggattt tgggacaggc cgatggatgt atcgctacga caccggaagc
     3361 tgcggaaatc tatcagcgcg cccgttggaa acacgatcct ggcacggttg cttttattcc
     3421 cacgccttat ccggtcgagg atcgacaatg gaacttttct gtcccgcctg acgagcaagc
     3481 agggattttt gtcgggacac gcgaatggga cgtgccgtca cgcaatcatt tcgctgcgct
     3541 gctggtcgcc cgacaacttt cggaagcgac cggggaaccg gtcacggtcg tcaatctgga
     3601 tggatacaag gcgcggcgat tgctttggga gctaaagttc ccggaagaaa agctctgtct
     3661 cgttgaagaa gaaaaatcgt atccggatta tctgcggatt gtggcccggc ataagcttgt
     3721 tctgcaactg gaccgcagcc gcgtgccagg ccaggtggca ggcgacgcgc ttctcacacg
     3781 gactgtgtgc gtgggcggcg atggcgcgat agaacgaatc gcgttcccaa agacctgcgg
     3841 cgaaggccgc agcatcgacg agatcgcctc gatcgcgctc gatctgctaa gaaatgctga
     3901 ttcgcgcgcc gcgattgtgg ctgaatcgca acagctcgca ctcgagagac tttcttttca
     3961 agccgtgcgt tcgcaacttg caaatttttt cgcccagttc gcaagcgcga gataagagac
     4021 agggatggtt cctctggtcg tgagacgcga tcaataaata ttgtagacgg tatgtgtcag
     4081 acgcctttca taaagcggcg tttcacagaa acgcccttca attggcaacc agtcgtcatg
     4141 cttcttgcag agtcaatcgc cccaccaatc gttcgtagat aaatatcttc caagcgctgc
     4201 gcctgcacgc gcaggtcgaa tctttctcga acgacttctg cgcccgcgtg cgcgatccgg
     4261 aaaagaaact tgggatcttc tgccgcgctc agcagcacgc gagccagttc ttcatgatca
     4321 tgctcaggca cgaggacgcc gctcacaccg ttcttgatcg cctcgggaat cccgccatgc
     4381 tcggtcgcga acaccggtag tccgctcgcc attgcttcca gcattgaatt tggaatccct
     4441 tcctgatttc cgtcggaacc ggtttcgctc ggatgcagga aaatgtgcga cgcataaaaa
     4501 atatcgcgca gttgttcctg cgaaacgaaa ccggtgaacg aaacgcgatt gtcgaccttc
     4561 agttcgcgcg ccagctgttg aagctggcct agcaatggac cttctccggc aatcgttagc
     4621 gtcgcgtttg gatattgcct cagaaaactc gcgaaggcgc ggagcgcaac cggcagacct
     4681 tttttctcga tcaggcggcc agcttgcacg aaccgccatt cgccattttg ggaagcgttg
     4741 cgttcacgaa acggaaattc atccagcgga atcccggttc gctggacttg tacttttttt
     4801 tcgtcgcaac cgagatcaat cactgcgcgg cgcaacgatt ccgagcgaac cagaaccagt
     4861 ttcaccgcgt tgagcatctg ccgcgtggcc tctcgataca acggcttgtg catgtcaacc
     4921 atcacgtcgg caccgtgaaa cgaaacaatc gaagggtttg accacgtcgg aatgagcggc
     4981 agcagatgca ccgcgatgtg accgaaataa atgtgcaaaa ggcgtgcatc ggttttttct
     5041 aggacaccca gcaacgcgcg aagctccgcg ccggaaattt gccagggcgc gtcgcgcagt
     5101 tgtctgaacc aaaagcggcg tagaaaatgc agcgccggtt ttgggacaat gtcgatctta
     5161 tcgaatggga agcggcccga gttttcgcgt ttctgagcga tgacaacagg agagatgcgc
     5221 tggagtgccg tgatttgccg atagatgtgc cacatctccg gtttgagaaa cgtcgcgcaa
     5281 taacaggcga caaccggggg aggatttttt gtcatgtcga gcggagtcga gacatctctc
     5341 actattcctc ttttaacagt aagagattcc tccacttcgg tcggaatgac aaaattccgc
     5401 gcgtgaggac acgagccaca tcaaacctca aacttcctag aagacgattt cgaaaagctt
     5461 gatttggtcg tatttccccg tgaacggatt ataagcgaga ctttgctcgc ccgtgccgta
     5521 ttgaggcggc gcccatggat tg
//
LOCUS       DASQUQ010000023         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_633726, whole genome shotgun sequence.
ACCESSION   DASQUQ010000023 DASQUQ010000000
VERSION     DASQUQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633726"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VE486_00615"
     CDS             <1..623
                     /locus_tag="VE486_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZA37619.1"
                     /translation="GEVVSAAGKSQLRIAETEKYAHVTYFFNGGIEKAFPGEDRKIVP
                     SPKVATYDLQPEMSAREVTDEVLAVMANYDLIILNFANPDMVGHTGILDAGIKAVETV
                     DECIARIIPKLLELDGKCIVTADHGNCEQMRNPDGSPNTAHTNYLVHCIYVAKDARKF
                     RVRDGILADIAPTLLFLLGLPQPKEMTGQNLLVPVSQTGATELGLP"
     gene            complement(660..1502)
                     /locus_tag="VE486_00620"
     CDS             complement(660..1502)
                     /locus_tag="VE486_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37620.1"
                     /translation="MDPEIDALGRTQVLLQAHAPSCPQSIGADSADTLQDSASTRRGE
                     SAAHRRLKRLALLWAQADGYSACALEVSMPRCRYRADVAAYRPARKKIGSTAIFECKH
                     ALSDLRRNNCRSQAARERLDVIGKRRQILEKYLRMHFPGLRVADSLFPEFDSHDFAII
                     GHRGYARVLRELKSLQNRLCGCTKFDKLIRYRCANLFFLVLPNELFRDSEIPVGWGAL
                     VESDGALTLMRKPVWQETAVETQVRLLQRIAMAGTRAVNRQLEITFADVLSTRSTSYR
                     STQR"
     gene            complement(1487..2473)
                     /gene="hemB"
                     /locus_tag="VE486_00625"
     CDS             complement(1487..2473)
                     /gene="hemB"
                     /locus_tag="VE486_00625"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZA37621.1"
                     /translation="MRKLVHRPRRLRRSPALRNLVRQTQVSAHDFILPLFVSEKLEGR
                     LPVASMPGVFQFSVKEIVGEAQRAQDLGLQAILLFGIPEQKDEQASAAYAENGIVQKA
                     LNAIKSKCPALVTITDVCLCEYMSHGHCGVTRIDGDHFHVLNDETVELLVKTALSHAA
                     AGADMVAPSDMMDGRIGAIREALDAAGFDQTGIMSYAAKFASAFYGPFRDAAESPPQF
                     GDRRSYQMDYANAEEALREVALDIDEGADVVMVKPALPYIDILWRVRERFGKPTAVYH
                     VSGEYAMVKAAADKGIVEERAAVLEIMTALKRAGADIIVTYWARELAEWIRK"
BASE COUNT          619 a          774 c          741 g          554 t
ORIGIN      
        1 tgggcgaggt cgtcagtgcg gccggcaaat cgcaactgcg catcgccgag acggaaaaat
       61 acgcacacgt aacctatttt tttaacggcg gaatcgagaa ggcgttcccg ggtgaggatc
      121 gaaaaatcgt accgtcgccg aaggtcgcca cctacgatct gcaaccggaa atgagcgcac
      181 gcgaggtaac cgatgaagtg ctcgcggtca tggcaaacta cgacctgatt attctcaact
      241 ttgccaatcc ggacatggtc gggcacaccg gcattctcga tgccggaatc aaagcggtcg
      301 aaaccgtgga tgaatgtatc gcgcgcatca tcccaaagct tctcgagctt gacggcaaat
      361 gtatcgtcac ggccgaccac ggaaattgcg agcagatgcg caatcccgac ggctcaccta
      421 acaccgcgca caccaactat ctcgtgcatt gcatctacgt cgcgaaagat gcgcggaagt
      481 tccgcgtccg tgacggaatc ctcgccgata tcgcgccaac tctcttattt ttgctcggac
      541 tgccgcaacc aaaggaaatg accggacaga atttgctcgt gccggtttcc cagacaggcg
      601 caaccgagtt aggactccca taaatcagag gattgcacgg cgtcggcaat agcccagcct
      661 taacgctgcg ttgaacgata cgaagtggaa cgtgtgctca gtacgtctgc gaacgtgatt
      721 tccagttgtc gattgaccgc gcgcgttcca gccatggcga ttcgctgtaa taagcgaact
      781 tgagtctcca cggctgtttc ctgccagacc ggcttgcgca tgagcgtgag cgcgccatcc
      841 gattccacga gcgcgcccca gccaaccggg atctctgagt cacgaaagag ctcgttcggc
      901 aggaccagga aaaacagatt cgcacagcgg tagcgaatca atttatcgaa cttcgtgcaa
      961 ccgcagagcc gattttgcaa cgacttcaac tcacgcaaaa cgcgggcata accacggtgt
     1021 ccgatgattg cgaagtcgtg cgagtcaaat tccggaaaga gcgaatcggc tacgcgtaaa
     1081 ccaggaaaat gcatccgcaa atatttctcg agaatttgcc gacgtttgcc gataacgtcg
     1141 aggcgttcac gcgcggcttg gctgcggcag ttattccttc gcaaatcgga aagcgcgtgc
     1201 ttgcattcaa aaattgctgt cgatccaatc tttttccgcg ccggccgata agcggccacg
     1261 tcggcgcggt accggcagcg gggcatgctc acttccagtg cgcacgcgga gtaaccatcc
     1321 gcctgcgccc agagcaacgc gagacgcttt aaacgcctat gcgcagcgct ctctccgcga
     1381 cgggttgatg cggaatcctg gagggtgtcc gcactgtcgg cgcctatgga ttgcggacac
     1441 gacggcgcgt gtgcttgcaa aaggacttgt gtgcgcccaa gcgcatctat ttccggatcc
     1501 attccgccag ttcccgcgcc caataggtga cgataatgtc tgcgccggcg cgtttgagcg
     1561 cagtcatgat ctcaagaacg gcagcgcgtt cctctacgat ccctttgtcg gctgctgctt
     1621 tgaccatcgc atattcaccg ctcacgtggt aaacggcggt cggtttgccg aaacgttcgc
     1681 gcacccgcca aaggatgtcg atgtacggca aagctggctt taccatcacg acgtcggcgc
     1741 cttcgtcgat atcaagagca acttcacgca gcgcttcctc cgcgttcgcg taatccattt
     1801 gataagaacg gcgatcgcca aattgcggcg ggctttcggc agcgtcgcga aacggcccgt
     1861 aaaaagccga cgcaaatttg gcggcataac tcatgatccc ggtttgatcg aaaccagcgg
     1921 cgtctagtgc ctcgcgaatc gcgccaatgc gaccgtccat catgtcgctc ggcgccacca
     1981 tgtcggcccc cgctgcggca tgggagagcg cagttttaac cagaagctct accgtctcgt
     2041 cgttgagaac atggaaatga tccccatcga tgcgcgtgac gccacagtgg ccgtggctca
     2101 tgtattcgca taagcagacg tcggtgatgg taacgagcgc cggacatttt gacttgatgg
     2161 cgttcagcgc cttttgaaca atcccgtttt cggcataagc ggcactggcc tgttcgtctt
     2221 tctgttcggg aatcccgaaa agaaggatgg cttgcagccc gagatcttga gcgcgctgcg
     2281 cttcaccgac aatctccttc acagaaaact gaaagacccc cggcatgctc gcgaccggaa
     2341 gacgcccctc cagtttttcg cttacgaaaa gcgggagaat aaaatcgtgg gcgctcactt
     2401 gtgtctggcg gacaaggttt cgcagggcag gggaacgccg caaacggcga ggccgatgca
     2461 ctaattttct cacaaacgaa gggtattcgg ttggtggagg cctcgcaagc tcgccagagg
     2521 acgaggctca acgtcgaaca tccaacatcg aacatccaat ctccaacggg cctgggttca
     2581 acgttcagtg ttggatgttg ggcgttggat gttttaataa ggagagattc ctccagcctt
     2641 cgcataaagc tacggcttgg caggcaactt cgctcggaat gacaaaga
//
LOCUS       DASQUQ010000024         6700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_456219, whole genome shotgun sequence.
ACCESSION   DASQUQ010000024 DASQUQ010000000
VERSION     DASQUQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456219"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VE486_00630"
     CDS             <1..812
                     /locus_tag="VE486_00630"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZA37622.1"
                     /translation="KGAVINLRGKFGPTVLGTDNVMMAAVLADGVTVVEGAAQEPEVV
                     DLANFLNKMGAKIEGAGTRRIIIEGVPALHGAEHDVIPDRIEAGTFLVAGAICGQGVT
                     VKRVEPEHIRSVTDALATCGFPVHIADHAISISPNGAASPLELETAPYPGFPTDMQAQ
                     MCALLSTSDGISVVTENIFPQRYMHVAELKRMGAQMQLEGATAVIHGVDGLFGAQVMA
                     SDLRASAALVLAGLKADGITEVSRVYHIDRGYEHLDEKLSELGAHIERVKE"
     gene            893..1276
                     /locus_tag="VE486_00635"
     CDS             893..1276
                     /locus_tag="VE486_00635"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HZA37623.1"
                     /translation="MDQQPVPPPPPVSAPSSDVRTWCVLCHAAALLGLFFHFLGHLLG
                     PLIVWLVKRGDSPEIDAHGKESLNFQISMLIYDAIAAILCLVLIGIPILIGLWILNTV
                     LVIIASIQASEGKFYRYPITIRLIK"
     gene            complement(1375..2277)
                     /locus_tag="VE486_00640"
     CDS             complement(1375..2277)
                     /locus_tag="VE486_00640"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HZA37624.1"
                     /translation="MQSGAVRQSTIQNRKSKILLGAHMSIGGGVQMSIQRACSIGCTA
                     MQIFVKNNMQWFARPLTCQEIGAFLKHSQRHELLAIFAHANYLINLAATNPQFHVNSL
                     RALSEELIRAHQLKLPFLVLHPGAHLGAGEEAGLEKIIESIDRVHHGIPKIETRIALE
                     TTAGQGSCLGSKFEQLAYIIARVREPERLCVCLDTAHIFAAGYDIGSESAVRKTFREF
                     DRVLGRDRLVAIHLNDSKTASGSRVDRHEHIGKGRIGLEAFRFIMRDRRFRKIPKVLE
                     TPKGKDLAEDVENLKTLRGLVREK"
     gene            complement(2323..4623)
                     /locus_tag="VE486_00645"
     CDS             complement(2323..4623)
                     /locus_tag="VE486_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HZA37625.1"
                     /translation="MRLVILAAILGILGTGWYLAKRGFGRQWRSLVVEELHKRGVEAS
                     VGRLTLDPFRGLVAKNVRIFDYKNRENTLALISEISLDINYAALIHHESFLNALDVRN
                     AQITLPLRTAEGRTEKVQLNNFRAHIYFPPEQIYVSQAEGIFCGIRISATGQLIKREN
                     YKPSPPVPEEEWRRRVAMVRWVVDELGKFKFPAAPPSLQVKFTGDVAEIENARVEATL
                     RGDRLQRGNYEISNLSAAAEWKDQHLDVTQCEWSDHLGSFAGRADWNQQSNRANFQIR
                     SSLDLRAFLDAFELGGPLVDVTFQSPPLIELSGSVIFEANRLRPELIGHAAFARFTYK
                     SVPFSDLAADFSWNGERTLLQDLRLRHQTGQFRADLFEAPDDFRLNVESTMAPGAVRA
                     VVSPGLDKFLRQWEWQRSPTIRLAIRGEDRNPENWRGDGTIAMERTRFRGVWLNSASA
                     KIHLADGAITCQDFRVVRDEGIGTGNFTYDFKKHEVRIANIKSSLHPAEVIFWIDPKL
                     WKTVVPYKFRRPPTVTANGVYQFAGGKNTRLEIKVDGANGMDYVFLGKTLPFDRVAGR
                     LLFTNDRLQIADLRGALLSGTLHGKTDISLARNDPRYQANISVSGIDFPRLTDLYYNY
                     KTAHGQMSGSYDFTGIGSDPRTMRGRGKVEVTNGDVFAIPIFGPLSGILNHIVPGSGY
                     SIAHQASATFKINDGIIHADDFEASSMLFSMLGHGDIHFLDDKLDFNLRLNMKGPGVL
                     LTPVYKLFEYTGEGSLKKPDWHPKRF"
     gene            4804..5253
                     /locus_tag="VE486_00650"
     CDS             4804..5253
                     /locus_tag="VE486_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HZA37626.1"
                     /translation="MSFSISDLPAINASLNLVSTVFISMGWYFIRHGAWRRHMTCMIT
                     AVVSSTFFLVGYITYHVHVGEKSSGYTGWIAAIYFPILASHVLLAFVTLPLVILTLIQ
                     VFRRRWDGHRRIARWTIPIWLYVSVTGVFVYLMLYKWFPPPNLALGP"
     gene            5299..5757
                     /locus_tag="VE486_00655"
     CDS             5299..5757
                     /locus_tag="VE486_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37627.1"
                     /translation="MVTIATFNEPDKAKRLKRRFHDAGLKADVHNEAPLQQVGFMSRP
                     QANAKVMVEDNDFEKAQSFMVEWEATDPDISAALIRCPQCGSSNIEYPQMTRKFMTPA
                     LVGVLCALKIIPKEFYCQDCHFTWSNQEEPTIGRLWHRFFPGLESGKEGS"
     gene            5934..>6700
                     /locus_tag="VE486_00660"
     CDS             5934..>6700
                     /locus_tag="VE486_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZA37628.1"
                     /translation="MKWLFVLLLALVIFGGAAWFGYNTFVKEEIEFKKEQRGEVPPEP
                     VPDFSLPEFQAAAKLRQEGKLAEARDALIAFIQKYPAGLHLGEAKDLLGEVNVDILLS
                     RYPSPEKTEYVVKPGDVLAKIARKLKTTPELIMRMNNMSGTMLRIGERLLISRPDFSI
                     AIQRKANLVVLLNHGVFFKQYHVREAKLPPKQPATITAKVAETMAFKGGKRVGLGSKE
                     YIGSTRWIRFAGAPAYTLYSTPDAAHPNLDQPPPPSGL"
BASE COUNT         1456 a         1810 c         1861 g         1573 t
ORIGIN      
        1 tgaagggtgc ggtaatcaat ttgcggggca aattcggtcc gacggtgctc ggcaccgata
       61 atgtgatgat ggcggccgtg ctcgctgatg gtgtgaccgt cgttgaagga gcagcgcagg
      121 agcccgaagt agtcgatctt gctaacttct tgaacaaaat gggtgcaaag atcgaaggcg
      181 ccggaacgcg gcggatcatc attgagggtg tgcccgcgct gcacggagcg gaacatgatg
      241 tgatccctga ccgaattgaa gcaggaacat ttctggtggc gggtgcaatt tgcgggcagg
      301 gagtcaccgt taagcgggtg gagccagagc atattcgctc ggtcacggac gcactcgcca
      361 cttgcggctt cccagttcac atcgcagacc acgcaatttc gatctcgccg aacggggctg
      421 cgagcccact ggagctagag actgcaccgt atcccggttt tcccaccgat atgcaggcac
      481 agatgtgcgc attactctcc acaagcgacg gcatcagcgt cgtgacggaa aatattttcc
      541 cgcagcgcta catgcacgtc gcggagctaa agcgcatggg agcgcaaatg cagctggagg
      601 gtgcaactgc ggtgatccac ggcgtcgatg gtctttttgg cgcgcaggtt atggcgagcg
      661 acctccgcgc ctccgccgcg cttgtgcttg cgggattgaa ggcggacgga attaccgaag
      721 tgagccgcgt gtatcacatt gatcgcggct acgaacatct cgacgagaaa ttgagcgaac
      781 tcggcgcgca catcgagcgc gtgaaagaat aaacgtgacg taagtctttg acttgcgttt
      841 tcccgcggca agctggacga ttgctgcaca ttctgaccga aaggatagat ttatggatca
      901 acagccagta ccacctccgc caccagtttc agctccctct tccgacgtac gaacctggtg
      961 cgtgctttgc cacgccgcag ctttgctcgg attatttttc cattttctgg ggcatctcct
     1021 cggtcccctc attgtttggc tggtgaaacg cggtgattcc ccggagatcg acgcccatgg
     1081 aaaagaatcg cttaatttcc agatttcgat gctgatttat gacgccattg ccgcaattct
     1141 ttgccttgtt cttatcggca ttccgattct gatcgggctt tggattctga acacggtcct
     1201 cgtcattatc gcctccattc aggcaagcga agggaagttc tatcgctatc cgattacgat
     1261 tcggctgatc aaataaatga cgaacgaaaa tgacgaagct cgaatgacca aatgacgaag
     1321 cccgattggc agcgcctttt cgtcattcct tcgtcattag gcattcgtca ttcgtcattt
     1381 ttccctgacg agcccgcgca acgtctttag gttctccacg tcttcggcga gatccttccc
     1441 tttcggcgtt tccagcacct tgggaatttt gcggaaacgc cggtcgcgca tgatgaaacg
     1501 aaaagcctca aggccaatcc tgcccttgcc gatatgttcg tgccgatcca cgcgcgagcc
     1561 gctagcagtt ttggaatcat tgagatgaat tgccacaaga cgatcgcgtc caaggacgcg
     1621 atcaaattcg cgaaatgttt ttcggaccgc tgattcgctt ccgatatcgt aaccggcggc
     1681 gaaaatatgc gcggtgtcca gacaaacaca gagccgctcc ggctcacgaa cccgggcgat
     1741 gatgtaagcg agttgctcga acttgcttcc caggcatgaa ccctggccgg cggtcgtttc
     1801 cagcgcgatc cgcgtctcaa tcttcggaat gccatggtgc acgcggtcaa tcgattctat
     1861 gattttctcg agcccggctt cttcgcctgc gccgagatgc gcgcccggat gcaacaccag
     1921 aaacggcagt tttagttgat gggcgcggat caattcttca gaaagagccc gcaacgagtt
     1981 gacatggaat tgcgggttag tcgcggcgag attgatcaga tagttcgcgt gcgcgaagat
     2041 tgccaataat tcgtggcgct ggctgtgctt gagaaacgcg ccaatctcct ggcaggtcag
     2101 cggccgggcg aaccactgca tattgttttt cacgaagatt tgcatggcgg tgcacccgat
     2161 cgaacaagcg cgctgaatcg acatctgcac gccgccaccg atcgacatgt gcgcgcccag
     2221 gagaattttc gattttcgat tttggattgt cgattggcgc acagcgccag actgcaatga
     2281 gcgcgtcgct ggcttcatca atcgaaaatc ggcaatgaaa atctaaaatc tcttggggtg
     2341 ccagtctggt ttcttcaagc ttccttcgcc cgtatattcg aaaagcttgt aaaccggtgt
     2401 gagcaacacg cccgggccct tcatgttgag ccgcaaattg aagtcgagtt tgtcatcgag
     2461 gaaatgaata tcgccatggc cgagcatgct gaaaagcatg ctggatgctt cgaaatcgtc
     2521 cgcatgaata attccgtcgt tgatcttaaa ggtcgcactt gcctggtggg cgatgctgta
     2581 tccgctgcca gggacgatat gattgagaat tcctgagagc ggcccgaaaa tcgggatcgc
     2641 gaacacatcg ccattggtca cttccacttt tccgcgccca cgcattgtcc gcggatcgct
     2701 gccgataccg gtgaaatcat agctgccgct catttggccg tgcgccgtct tgtaattgta
     2761 gtacaaatcg gtcagccgcg gaaaatcgat tccgcttacg gagatattcg cttgataacg
     2821 gggatcattt cgcgccaggg agatgtcggt tttcccatgc agcgtgccgg aaagaagggc
     2881 gccgcgcaga tcggcaatct gcaatcggtc gttggtgaaa agcaatcgtc ccgcgacccg
     2941 gtcgaaaggc agtgtcttcc cgaggaagac gtaatccatc ccattggcgc catcgacctt
     3001 gatctccagg cgggtgttct tgcctccggc aaactgatat acgccattag cggtaacggt
     3061 gggcgggcgt cggaatttgt acggcacaac agtcttccaa agcttggggt cgatccaaaa
     3121 aatcacctcg gcgggatgca acgagctttt gatatttgcg atgcggactt cgtgcttttt
     3181 gaaatcgtag gtgaagttgc cggtgccgat tccttcatcg cgcactacac gaaaatcctg
     3241 gcaagtgatg gcgccatcag cgagatgaat tttcgcgctc gcgctattta accatacccc
     3301 gcggaatcgg gtccgttcca tcgcgatggt tccatcgcct cgccaatttt ccggattgcg
     3361 atcctcgcct cggatcgcga gacgaatagt gggcgaacgc tgccactccc attgccgaag
     3421 aaatttatcg aggcccggag agacaactgc ccgcactgcg cctggcgcca ttgtgctctc
     3481 gacgtttaac cgaaaatcgt cgggggcctc gaagagatcg gccctgaact ggccggtttg
     3541 atgtcgcagg cgcaggtcct ggagcaatgt gcgctcgcca ttccacgaaa aatccgctgc
     3601 gagatcagag aacggcacgc ttttataagt aaaccgggca aaggcggcgt ggccgatcag
     3661 ttccggtcga aggcgattcg cctcgaaaat taccgagccg gagagttcga tcaggggcgg
     3721 tgactgaaat gtcacgtcca cgagcggacc gccgagctcg aaagcatcga gaaaagccct
     3781 gagatccagg ctgctgcgta tctgaaagtt cgcgcggttg ctttgctggt tccaatcagc
     3841 gcggccggca aagctgccca ggtgatcgct ccattcgcac tgcgtaacgt ccagatgctg
     3901 gtccttccac tcggcggcgg cagacaggtt gcttatttcg taattgcctc gttgcaaccg
     3961 atcgccccgc agcgtcgctt cgactcgtgc attctcaatt tctgcgacgt ctccggtgaa
     4021 cttcacctgc agcgacggag gcgcggccgg gaatttgaac ttccccagct catccacaac
     4081 ccatcgcacc attgcgactc gccttcgcca ttcttcttct ggaaccggcg gcgatggctt
     4141 gtaattctcg cgcttgatca actgtccagt ggccgaaatc cgaattccgc aaaaaattcc
     4201 ctcggcttgg ctgacgtaaa tttgttccgg aggaaaatag atatgggcgc ggaaattatt
     4261 gagttgcacc ttctcggttc gtccctccgc cgtcctgagt ggaagcgtga tttgcgcgtt
     4321 ccgcacatcg agcgcgttga gaaatgattc atgatgaatc agtgcagcgt aattaatatc
     4381 gagtgagatt tcgctgatga gggccagcgt gttctcgcgg ttcttatagt cgaaaattcg
     4441 gacatttttc gcgacgagtc cgcggaacgg gtccagagtc aatcgtccca ccgaggcttc
     4501 gacgccgcgc ttgtgcagtt cctcgacgac aagcgagcgc cactgccggc cgaagcctct
     4561 ttttgccaga taccagccgg tgccgagaat cccgagaatt gcggcgagaa tcaccagccg
     4621 cattacccac acggcggttc gatggatcca aatcggtcgt cgatcttgcg cgggaggccg
     4681 gacatggggg gcttcgagca cgggcggcat aatttcgttt aactcgttga agattaaatc
     4741 gttgaaatat taaatcgtaa ggcgtttgcc gatgtaacgg tttatcgttt taacgattta
     4801 acaatgagct tttctatttc cgatcttccg gcaattaacg cttccctcaa tttggtgagc
     4861 actgttttta tttcaatggg ctggtacttc atccggcatg gcgcgtggcg gcgtcatatg
     4921 acgtgcatga tcacggctgt ggtttcatcc acattttttc tcgtcggcta catcacttat
     4981 cacgtgcacg tcggcgaaaa atcgtcgggc tacacgggat ggatcgccgc gatttacttt
     5041 ccgattttag cgtcgcatgt tctgctcgcc tttgtcacgt tgcctctggt gattttgacg
     5101 ctgattcagg tttttcgtcg ccggtgggac ggccatcgac ggattgcgcg ttggaccatc
     5161 ccgatctggc tctatgtttc agtcaccggg gtgtttgtct atttaatgct ctacaaatgg
     5221 ttcccaccgc caaatcttgc actcggccct tagttgttgt ttgatctgac gacgcggttt
     5281 cgcgcaacga caaaatttat ggtaacgatc gcgacgttta atgaaccgga caaagctaaa
     5341 cgcctcaaac gacgtttcca cgatgcggga ctgaaggccg atgttcataa tgaagcgccg
     5401 ttgcagcagg tcggcttcat gtcgcgtccg caagcgaatg caaaggtgat ggtggaggac
     5461 aacgacttcg aaaaggcgca aagctttatg gtcgagtggg aggcgaccga tcccgatatt
     5521 tccgccgcgt tgattcgttg cccgcaatgc ggctcttcaa acatcgagta tccacagatg
     5581 acccggaaat tcatgacgcc ggcgctggtt ggtgtgctgt gcgcgctcaa gattattccc
     5641 aaggagttct attgtcagga ttgccatttc acctggagca accaggagga accgacgata
     5701 ggccgcctct ggcaccgatt cttcccggga ctcgaatctg gcaaagaggg ttcgtaggtt
     5761 agcaggctta gattgaccgg cgcgctgcac gcctcgcgga cgattcttgt tgtgatggat
     5821 cttcgattgc ccggaacaat acgaccctac ggttcgcccc cggatcgcgc gaccgcttgc
     5881 aggtgtcgga tttcgctcgg attcacccgc ctgaggcggg cactctgcca tccatgaagt
     5941 ggctctttgt gttgttgctg gcgctggtca tcttcggagg ggccgcctgg ttcgggtaca
     6001 acactttcgt caaagaagaa atcgagttca aaaaggagca acgcggagag gttcctcctg
     6061 agccggtgcc ggatttcagt cttcccgagt tccaggcagc ggcgaaactg cgacaggaag
     6121 ggaaactggc cgaagcacgc gacgcgctga ttgcctttat ccaaaaatac cctgccggtt
     6181 tgcatctggg agaagccaaa gacttgctcg gcgaagtgaa tgtggacatt ttgctctcgc
     6241 gttatccgtc tccggaaaaa acggaatacg tggtgaagcc cggagatgtg ctggcaaaga
     6301 ttgcgcgcaa gctgaaaacg acgccggagc tcatcatgcg catgaataat atgagcggca
     6361 cgatgctgcg catcggcgaa cgtctgctca tatcgcgccc ggatttctca atagcaattc
     6421 aacggaaggc gaacctggtt gtgctcctaa atcatggcgt ctttttcaaa cagtatcatg
     6481 tgcgtgaggc gaaattaccg cccaaacagc ccgcgacaat cacggcgaag gttgctgaga
     6541 caatggcttt caaaggtgga aaacgcgtgg gcctcgggag caaggaatac attggcagca
     6601 cccgctggat tcggtttgcc ggagcgccgg cttacacatt gtattccacg cccgatgccg
     6661 cgcatccgaa tctcgatcag ccgccgccac catcagggct
//
LOCUS       DASQUQ010000025         5828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_183987, whole genome shotgun sequence.
ACCESSION   DASQUQ010000025 DASQUQ010000000
VERSION     DASQUQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183987"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..483
                     /locus_tag="VE486_00665"
     CDS             205..483
                     /locus_tag="VE486_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37629.1"
                     /translation="MKPTFITTRSQKRFPLIECNYHSAVLSDYRGRCVKPTAPSFRNI
                     SRAYFQKEARHDFFGETILFAMLSITAAVPLVSGGYAIIQLCRAFGAL"
     gene            491..1816
                     /locus_tag="VE486_00670"
     CDS             491..1816
                     /locus_tag="VE486_00670"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZA37630.1"
                     /translation="MTPQRQPKESTIVRPAGFTPNLSSMDVPREGVAAKKRKRRIIII
                     AASALGLILATIAISRLKPAVPSVDRSTVWIDTVKRGPMVRQVRGLGTLVPEEIRWLP
                     ANTEGRVEKIVVWPGTPVEPDTVILELTSPELEQTAHDAELQATAAEAELTSLRATLQ
                     REVLDQESTTARVHSEYEQAKMERQTNDQLAKNGLIAELDYKTSKVKEAELANRDEIE
                     QKRLAFSRDSIEPQIASKQAAVDQAKQLAKLKSDQVEALHVRAGMSGVLQQLPVQIGQ
                     RVKVGDNLARVADPTKLKAQVKIAETQAKDIQINQQAVIDTRNGTVNGHVTRVDPAVE
                     QGTVTVDVAFDDALPKGARPDLSVDGTIELERLDNVVYVGRPAFGQENNTVGMFKLVG
                     GSSEASRVPVKLGKSSVNTIEILDGLQPGDQVILSDTSAWDAHERIRLN"
     gene            1852..2265
                     /locus_tag="VE486_00675"
     CDS             1852..2265
                     /locus_tag="VE486_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZA37631.1"
                     /translation="MNAQDLKDRTKEFALRVMRLVDALPNTPKGRAIASQLVRSGTSV
                     AANYRAAGRGRSHAEFISKIGVAEEEADETGLWLELIINDKLLPEKKIASLPKEANEL
                     TAIMAASYISASRAKRNGAANSKSRISNQQSAIRT"
     gene            2262..3005
                     /locus_tag="VE486_00680"
     CDS             2262..3005
                     /locus_tag="VE486_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA37632.1"
                     /translation="MTTNALNPHTSSQTSDGNGSTPLIRLNGVRKVFLTDEVETHALS
                     GIHLDIRTGEYVSIAGPSGCGKTTLLSILGLLDTPSDGSYSLKGTEVANLSFADRARI
                     RNREIGFIFQSFNLIGDLTVFENVELPLTYRGMAGGERKSFVTEALERVGMAHRAKHL
                     PSQLSGGQQQRVAVARALAGRPAILLADEPTGNLDSRNGEAVMELLKELHAVGATICM
                     VTHDERFAQHADRTVHLFDGRVVEDRMAA"
     gene            3027..5531
                     /locus_tag="VE486_00685"
     CDS             3027..5531
                     /locus_tag="VE486_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA37633.1"
                     /translation="MFFESFFQDLRVGLRVLAKEKSFCFLAVLVLALGIGGATTQFTV
                     VNAIALRGFSFPHPEQLMSVGLIDPQASAQNNNFGFGVIPTAQDYEDLRAAQQSFAQM
                     AGYLNGSTINLTYKNNPQRYTGGYVTEDFFKIIGVSPILGRDFTAEDNKPGAEKVTIL
                     GHEIWQRDFSADPNIVGQSVRINGKAATVIGVMPPNFKFPVSEELWVPLYNEFPPKPR
                     GELLLGASNAAPAVMGRLKTGVSIDQANAEFVGLARHLAQDNPKTNQNLVSASVQPLL
                     HAMTGVQLRQTVWAMLAAVIFVLLIACVNVMNMQFGRAALRAKEVAIRGALGATRWRI
                     VRQMLTESFVVAVFGAVAGILLAYWAVDLFVRATNSLPAPLPYWVQFKIDGPVLVFTV
                     AITLVAALVSSLVPALISAKGNAAEMMKEGGRGNTSRLVNIITRVLVVGQIALTAALL
                     IASTLQIKSIRNQLKLNYGYDEDAVYAARMGLMEGAYPTHDARREFFLRAVRALRANP
                     QFESAAMSSRFRMTFDGQGQYEVDGQNYVTDRDRPRGNYESVSDNYFSTLGLKILEGR
                     DFTIDDADSKQPVAIVNTSFARKYYGNESPIGRRIRIFNPGKPQPWRTIVGVVPDTLM
                     QGPFDQQTENAGFYMPLLGAEPAPQFCTILVRPRSGQRADTLGPALSKAVAEVDSNLP
                     TYFAGTPARFHNEILGANRIIATLFSIFGAVAFLLSAVGLYGVMSFSVNQRTQEFGIR
                     MALGADARRIFRMVMTQGAWQLILGLVLGASGAALLLGVLAAAALQNILFKVNALDPF
                     IYFLVAGLLTIVAAASCFVPARRATLVDPIEALRTE"
     gene            5533..>5828
                     /locus_tag="VE486_00690"
     CDS             5533..>5828
                     /locus_tag="VE486_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37634.1"
                     /translation="MNDLRFALRQLRKSPGFTLLAVITLALGIGMNTAIFSLIQDLFL
                     RGLPFSEPDRLVRIYGESKERDLKQMPSSVPKFWHYREGQTVFSSIAADWGNGF"
BASE COUNT         1315 a         1590 c         1640 g         1283 t
ORIGIN      
        1 ccaggttgcg tcccaaaagc gggacgaggc agtcccaaaa gcgaagcgaa ttacgccacg
       61 tgtgttcaac atcgacactc ctctagtcac taatcacgag tgacttatca ctttcttcgc
      121 gacgttggca cgggcattgc aatacctatt gtcgaccaga agaacttttg aatccaacaa
      181 ctacccgcaa cgcatcttaa gagtatgaaa ccaacgttca tcacaactcg cagccagaag
      241 aggttcccgt tgattgagtg caattatcat tccgcggtgc tcagcgatta tcgcggccgt
      301 tgcgtaaaac cgacggcgcc ttcttttcgc aatattagcc gggcgtactt tcaaaaggaa
      361 gcacggcacg atttcttcgg cgaaacgatt ttgtttgcaa tgctgagcat caccgcggcc
      421 gtgccgttgg tgagcggagg gtacgcgatc attcaactct gtcgcgcttt tggagcgctt
      481 tgatcaggcc gtgactcccc agcgacagcc taaggaaagt acgattgtgc gaccggccgg
      541 attcacgccg aatctttcgt cgatggacgt gccacgcgaa ggggttgcgg cgaagaaacg
      601 caagcgccgc atcattatca tcgccgcttc cgcgcttgga ttaattctgg ctacgattgc
      661 gatctcgcgt ttgaaaccgg cagtgccgag cgtcgatcgc tccacagtct ggatcgatac
      721 ggtgaaacgc gggccgatgg tacgccaggt gcgcggattg ggcacactgg tgccggaaga
      781 aattcgctgg cttccggcaa acaccgaggg acgcgtcgaa aaaattgttg tctggcccgg
      841 cacacccgta gaacccgata cggttatttt ggaattgacc agcccagagc tggagcaaac
      901 cgcgcacgac gccgaattac aagccacggc ggcggaggca gagctgacga gtttgcgcgc
      961 gactctccaa agggaggttc tcgatcagga atcaacgact gcgcgcgttc attctgagta
     1021 cgaacaggcc aaaatggaac ggcagaccaa tgaccaactg gctaagaatg gtttgattgc
     1081 cgagctcgac tacaagacgt cgaaagtaaa agaagcggag ctggcaaatc gcgacgaaat
     1141 cgaacagaag cgactagctt tttcacgtga ttcgattgaa ccccagatcg cttcaaaaca
     1201 agcagcggtc gatcaagcga agcagcttgc gaagctaaaa tcggatcaag tagaagcgct
     1261 ccacgttcgc gccggaatgt ctggcgtatt gcagcaattg ccggttcaga tcggacagcg
     1321 ggtgaaggtt ggcgataatc tggcccgtgt cgctgatccg acgaaactca aagcgcaggt
     1381 taagatcgcc gagacgcagg cgaaagatat ccaaatcaac cagcaagcgg tgatcgatac
     1441 acggaacggc actgtgaacg gccatgtcac ccgggtcgat ccggcagtcg aacagggaac
     1501 agtcactgtc gatgtcgcgt tcgatgacgc tctgccaaaa ggagcgcggc ccgatctgag
     1561 cgtggatgga acaatcgaac tcgagcgtct cgacaatgtc gtttatgtcg gccgcccagc
     1621 gttcggacag gaaaacaata ccgttggaat gttcaaactt gtcggcggca gctcggaagc
     1681 ttcgcgcgtg ccggtaaaac tcggcaagag ctccgtgaat acgatcgaga ttctggacgg
     1741 ccttcaaccg ggagatcagg tgattctttc cgataccagc gcatgggatg cgcacgaacg
     1801 catcagactg aactgattga tgatttctga ttgctgatag ctgattttca aatgaacgcg
     1861 caagatctta aagaccgcac caaggaattt gcgctgcggg ttatgcgttt ggttgacgcg
     1921 ttgccaaata cgccaaaagg ccgcgcaatt gccagtcagc tggttcgaag cgggaccagc
     1981 gtagcggcga actatcgcgc cgccggccgc ggacggtcgc acgcggaatt tatctccaag
     2041 atcggcgtcg ccgaagaaga agctgacgaa acaggccttt ggttggaatt aatcatcaat
     2101 gataaacttt tgccggaaaa gaaaattgct tctctgccga aggaagcaaa cgagctgact
     2161 gccatcatgg cggcttcgta catctctgca tcgcgcgcta agcgcaatgg cgcggcaaat
     2221 tcaaaatcaa gaatcagcaa tcagcaatca gcaatccgaa catgacaacg aacgcactta
     2281 atcctcacac atcatctcaa acctccgacg gaaacggctc gactcccttg atacgattga
     2341 atggcgtaag gaaggttttc cttaccgacg aagtggagac ccatgcgctc tccgggatcc
     2401 atctcgacat ccgtaccggc gaatatgttt cgatcgctgg accatccggt tgcggcaaga
     2461 cgacgttgtt gtcgatcctc ggcctcctcg atactccttc cgacggcagc tactccttaa
     2521 aaggaaccga agttgctaat ctttcgtttg ccgaccgcgc ccgaattcgc aatcgcgaga
     2581 tcggttttat tttccaaagt tttaatctaa tcggcgatct caccgtcttt gagaatgtcg
     2641 aattgccgct cacctatcga ggaatggcag gtggtgagcg caaatcattt gtgacggagg
     2701 ccttggagcg cgtgggcatg gcgcaccgtg caaaacacct gccaagccag ctctccggtg
     2761 gacaacagca gcgagtggca gtcgcgcggg ctctcgcggg caggcccgca attttgctcg
     2821 cggacgagcc gacgggaaac cttgattcgc gcaacggcga agcagtgatg gagctgctca
     2881 aagagttgca cgccgttggg gcgacgatct gcatggtcac gcacgatgag cgtttcgcgc
     2941 aacacgccga tcgtaccgtg cacctatttg atggccgagt cgttgaggac cggatggcag
     3001 catgaggaaa tttctgcctg ccccatatgt tcttcgaatc attcttccaa gatctccggg
     3061 tcggtcttcg tgttctagct aaggagaaat cgttttgttt tctggccgtc cttgtgctcg
     3121 ccctgggcat cggcggcgca accacgcaat tcacggtcgt taacgcgatc gcgctgcgcg
     3181 gattttcgtt cccgcatccc gaacagctga tgagcgtcgg cttgatcgat ccccaggcca
     3241 gcgcccaaaa caataatttc ggatttggcg tcatcccgac ggcgcaggat tacgaggatt
     3301 tgcgagcggc gcagcagtcg ttcgcgcaaa tggcggggta tctgaatgga tcgacaatca
     3361 acctgacgta taaaaacaat ccacagcgtt acaccggcgg gtacgtgacc gaggacttct
     3421 ttaagatcat cggcgtatcg ccaatcctcg gtcgcgattt caccgcggag gacaataagc
     3481 cgggcgcgga aaaggtcacg atcctgggcc acgaaatctg gcagcgcgat ttcagcgccg
     3541 atcccaatat cgtcggacaa agcgttcgaa ttaatggcaa agccgcgacc gtcattgggg
     3601 tgatgccgcc taatttcaaa tttccggttt ctgaagaact gtgggtccca ctttacaatg
     3661 agtttcctcc caaaccgcgc ggggagttgt tgcttggagc cagcaacgcc gcacctgcgg
     3721 tgatgggtcg attgaaaacc ggcgtgagca tcgatcaggc gaatgcggaa tttgtcggcc
     3781 tggcacgcca tttggctcag gacaacccga aaacgaatca aaacctggtc tcggccagcg
     3841 tgcagccact cctgcacgcg atgaccggcg tgcaactgcg ccagacggtt tgggccatgc
     3901 tggcggcagt cattttcgtt ttgctaatcg cctgcgtgaa cgtgatgaac atgcaattcg
     3961 gccgggctgc tttacgggca aaggaagtgg cgattcgcgg tgcgctcggg gccacccgtt
     4021 ggagaatcgt ccgccaaatg ttaaccgaaa gtttcgtggt cgccgtattc ggcgcggtcg
     4081 cagggatatt gctggcttat tgggcagtcg atctgttcgt ccgcgcgaca aactcgctgc
     4141 cggctccact gccctactgg gttcaattca aaatcgacgg ccctgttctc gtcttcactg
     4201 tcgcaatcac gttggtggcg gcgctggttt ctagtttggt cccagcgctg atcagtgcga
     4261 agggcaacgc ggctgagatg atgaaagaag gcgggcgggg aaataccagc cgtctggtca
     4321 atatcatcac gcgcgttctc gttgtcgggc agatcgcgtt gacggccgcg cttctgatcg
     4381 cctcgactct gcaaatcaaa tcgatccgaa atcagctaaa gctcaactac ggttacgacg
     4441 aggacgctgt ttacgccgcg cgcatgggct tgatggaagg cgcttatccg acgcacgacg
     4501 cgcggcgtga gttttttctt cgcgctgtac gcgcgttgcg ggccaatcct caattcgagt
     4561 ccgccgccat gagcagtcgc tttcgcatga cctttgacgg tcagggtcaa tatgaagtgg
     4621 acggccaaaa ttatgtgacc gaccgcgatc ggccacgagg caactatgaa tccgtctcgg
     4681 acaattactt ttcgacactt ggcttgaaga ttttggaagg ccgcgacttc accattgatg
     4741 atgctgattc gaagcaaccg gtggcgatcg tgaacacgag ttttgcgcgc aaatattacg
     4801 gaaacgaaag cccaatcggt cggcgaattc ggatttttaa tcccggcaag ccgcaaccgt
     4861 ggcggacgat tgtcggcgtt gtgcctgaca cgctgatgca ggggccattc gatcaacaaa
     4921 ctgagaacgc aggcttttac atgccgctac tcggcgccga gccggcgcct caattttgta
     4981 ccatccttgt tcgtccgcgt tcggggcagc gtgctgatac gctcgggccg gccctgagca
     5041 aagccgtcgc tgaagtcgat tcaaatttgc cgacctattt cgcgggcacg ccggcgcggt
     5101 tccacaatga aattctcgga gccaaccgca ttattgccac cttgttcagc atcttcggag
     5161 ctgttgcatt tcttctatcg gcagttgggc tctacggcgt gatgagtttt tcagttaatc
     5221 agcggacgca ggagtttggt attcggatgg cccttggtgc ggacgcgcgc agaatttttc
     5281 ggatggtcat gacccagggc gcgtggcagt tgatcctcgg ccttgtcttg ggcgccagcg
     5341 gcgctgcgtt actcctcggc gttcttgccg cggcggcgtt gcaaaacatt ctcttcaagg
     5401 tgaacgcgct cgacccgttc atttattttc tagttgcggg gttgctcaca atcgttgcgg
     5461 ctgcctcctg ctttgtgcca gctcggcgcg cgacattggt cgatccaatc gaagcattgc
     5521 ggaccgagtg atatgaacga ccttcgcttc gccctgcgcc aactgcgcaa atcgccggga
     5581 ttcactcttc tcgccgtcat cacgctcgcg ctcgggatcg ggatgaacac cgccatcttc
     5641 agtttaatac aggacctttt cctgcgcggt ctgcctttct ctgaaccgga ccgactcgtt
     5701 cggatttatg gcgagtcgaa agagcgcgat ttgaaacaaa tgccgtcttc ggtgccgaag
     5761 ttttggcatt atcgagaggg acagaccgtt ttctcgagca tcgccgcgga ttggggcaac
     5821 ggcttcat
//
LOCUS       DASQUQ010000026         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_457980, whole genome shotgun sequence.
ACCESSION   DASQUQ010000026 DASQUQ010000000
VERSION     DASQUQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457980"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VE486_00695"
     CDS             complement(<1..534)
                     /locus_tag="VE486_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HZA37635.1"
                     /translation="MDLNSLIERKRERFEQLEREIADPHLFENRKRAGEIMREHAGIK
                     DLLARWDRLEAARRQLDDNRELATSRDVEIAAIADDEIPDLEERVRDLEREIQIGLLP
                     PDENEDRDAIVEVRAGTGGSEAAIFAADLYRMYNRYAEAGGLKTEDLESSPSELGGLK
                     EVIFRVSGESVFRKLRYE"
     gene            complement(608..835)
                     /gene="rpmE"
                     /locus_tag="VE486_00700"
     CDS             complement(608..835)
                     /gene="rpmE"
                     /locus_tag="VE486_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZA37636.1"
                     /translation="MKAGIHPEYYDTDIVCACGAVYHTRSTRRDIKIGICAACHPFFT
                     GEQKFVDTAGRVEKFARRYGSTKAARAEKKA"
     gene            886..2286
                     /locus_tag="VE486_00705"
     CDS             886..2286
                     /locus_tag="VE486_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZA37637.1"
                     /translation="MKCRDPLLATWSGILARKGGRAAIFEPTGQIARTFQQIDERARH
                     FEAEIEMFNAGSVIAIQTGNHEDWPSILIACLRKRLVALPLEQSISDQRRDAALKICQ
                     AAAVVSAVPSGSSPKIFRLRTADATVDWGESPPAILKLTSGTTAAPRAIRFRSEQLLA
                     DCSQICDTAGIGDADLNFGVIPISHSYGFSNLLTPLIARGVPMVLSRDRMPRAVLADL
                     ARTNATVFPGMPVFYQAFCEMENIPELPKLRLCISAGAPLPVSVARQFRQKFRMAIHS
                     FYGASECGGICYDADGTSEVEGFVGQPIKGVAIAMIDPAASASQIRVRSAAVGDGYFP
                     EPAEAKLGGGVFVPDDLLTKTAKGWRIVGRTSDVINVAGKKVNPAEVESHLLRFSGVR
                     QAVVFGRASALRNEEVAACVVASPGVSEGELLEFCRRELSGWQVPKRVFIVDAIPVNE
                     RGKISRPELARRFAGL"
     gene            2415..>3238
                     /locus_tag="VE486_00710"
     CDS             2415..>3238
                     /locus_tag="VE486_00710"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZA37638.1"
                     /translation="MPNSSGEPKPTEAQGEPLTSEEQDQTSSERIPRETETAAQEPAT
                     DSESAVQSEDWWIPSPPVGELAKPANANFMPDVEVDASKEAATADVYFCGRTTLFPLN
                     RALQAIVREELTGVLRAFWDQDPIELLTKDGRIVYATMRNPELYCSETPAVLAKVGEE
                     VIAQARAEQSETGTPFFLTLAQQESIARQPAMELVQHYGQKLFSQLWAAPRVWIMFEK
                     NAGLLSEAPEVSAEQTVDNWALETLRFVEDLDQHVKYDPTSIPAYTKEGFERVQKL"
BASE COUNT          698 a          878 c          962 g          700 t
ORIGIN      
        1 ttcgtaacgc agttttcgaa aaacagattc gccggacacg cgaaagatca cttcttttaa
       61 gccaccgagc tccgacggac tcgattcgag gtcctcggtt tttagcccgc ccgcttccgc
      121 atagcgattg tacatccgat aaaggtcggc cgcgaaaatc gccgcttcgc tgccgcctgt
      181 cccggcgcga acttcgacaa ttgcgtctcg atcctcattc tcatctggcg gaaggagacc
      241 gatttgaatt tcgcgttcga gatctctaac tcgttcttcc aaatctggga tttcgtcgtc
      301 ggctatcgcc gcgatctcga catcgcggga agtggcgagc tcgcgattgt cgtccagttg
      361 acgccgcgca gcttcgagtc gatcccagcg cgcaagcaag tccttgatgc cggcatgttc
      421 gcgcatgatt tcgccagcac gcttgcggtt ctcaaacaga tgcggatctg cgatttcgcg
      481 ttcgagttgt tcgaagcgct cgcgtttgcg ttcaatgagg gaattaagat ccatacgtgg
      541 ttcctatccc gcggcgctgc atcgcccccg ggcggcgcct cgacagagcg aggccgctac
      601 agttcagcta agctttcttt tccgcccgcg cggctttagt gctgccgtag cggcgagcga
      661 atttttcgac acgcccggcg gtatcgacga acttctgctc gccagtgaaa aagggatgac
      721 aagcggcaca aataccgatc ttaatatcgc gacgcgtcga gcgggtgtga tataccgcgc
      781 cgcacgcgca cacaatatcg gtatcgtaat actctggatg gatgcctgct ttcatgttgg
      841 gaaacgagcg cggaacctac ccgcgggaag ctgctcgcgc aagggatgaa atgccgggat
      901 ccattgctcg cgacatggag cggaattctg gcgagaaaag gcggtcgggc cgccattttc
      961 gagccaaccg gccaaatcgc acggacgttt caacaaattg acgaacgagc gcgccacttc
     1021 gaagcggaga tcgaaatgtt caacgcgggt agcgtcatcg cgatccagac tggaaaccac
     1081 gaagattggc cgtcgatttt gatcgcttgt ttgcgcaagc ggctggttgc tttgcctctg
     1141 gaacaatcaa tcagcgatca acggcgcgat gctgcgctga aaatctgcca ggcggcagct
     1201 gtagtgtccg ctgtccccag cggaagctcc ccaaagatat ttcgactgag gacagccgac
     1261 gctacagtgg attggggcga aagcccgccg gcaattctaa aattgacttc cggcacgacg
     1321 gctgcgccgc gtgccattcg attccgtagc gaacagctcc tcgccgattg cagtcagatt
     1381 tgcgacaccg cgggaatcgg cgatgccgat ttgaatttcg gggtcattcc aatttcgcat
     1441 tcttatgggt tcagcaattt gctcacgcca ctgattgccc gtggcgtgcc gatggtgttg
     1501 agccgcgacc ggatgccgcg cgctgtgctc gcagatcttg cccgcactaa cgcaacggtt
     1561 ttccccggaa tgccagtttt ctatcaggca ttctgcgaga tggaaaatat ccccgaactg
     1621 ccgaagttgc ggctctgcat ttccgccggt gcgccgctgc ctgtgagcgt ggcgcggcag
     1681 tttcggcaaa aattcagaat ggcgatccat tcgttttacg gcgcatcgga atgcggcgga
     1741 atctgttatg atgcggacgg aacaagcgaa gtggaaggtt ttgtgggtca accaataaaa
     1801 ggcgtcgcta ttgcgatgat cgatccagca gcctcagcga gccagatccg cgtgcgcagc
     1861 gccgccgtgg gcgacggtta ttttcctgaa ccggccgaag ccaaacttgg cggaggagtt
     1921 tttgtgcctg acgatttgct aaccaagacg gcgaaggggt ggcgaatcgt tggccggact
     1981 tcggatgtaa taaatgtcgc cggtaaaaag gtgaatccgg cggaagtgga atcgcactta
     2041 cttcgcttca gcggcgtgcg tcaggcggtg gtatttgggc gcgcgtcggc tttacggaat
     2101 gaggaagtgg cggcatgcgt ggtcgcctcg ccaggggtga gcgagggtga actactcgaa
     2161 ttttgcagac gcgaattgag cggctggcag gtcccgaagc gggtttttat tgtggatgca
     2221 attccggtga atgagcgcgg aaaaatcagc cggcccgagc tcgcgcgtcg tttcgcgggg
     2281 ttgtagccgc ggcgctgtat cgccgtgtca ggcgaacgcc cacatggatc aggagagatc
     2341 gccgttgcga tgatcgggtt cttgagtaaa gccttcaagg gagcgttgct ttattaaaac
     2401 cgtggaggac aactatgccg aactcatcgg gcgaacctaa accgacagaa gctcaaggcg
     2461 aacccttaac tagcgaagag caggatcaaa ccagcagcga gcggattcca cgggagacgg
     2521 agactgcggc ccaagagcct gctaccgatt cagaaagtgc cgtccaatcc gaagactggt
     2581 ggatcccatc cccaccagtc ggcgagttgg ccaagccagc caacgccaat ttcatgccgg
     2641 atgtggaagt cgacgcttcg aaggaggccg ccacggcgga tgtttatttt tgcggacgca
     2701 caaccttgtt tcccttgaat cgcgctcttc aagcgatcgt cagggaggaa ctcacgggcg
     2761 tgttgcgggc attctgggac caggatccaa tcgaactttt gacgaaggac ggcaggattg
     2821 tttacgccac gatgcgaaat cccgagcttt attgttcgga aacgcctgct gttctcgcca
     2881 aagtcggtga ggaagtgatt gcgcaggcac gcgccgaaca gagtgaaacg ggaacgccgt
     2941 tttttcttac tctggcccag caggaatcga tcgcgcgcca gcccgccatg gagttagtcc
     3001 agcattatgg ccaaaaactc ttctcccaac tttgggccgc gccgcgggtg tggatcatgt
     3061 ttgagaagaa cgccgggctg ttgagtgaag ctcccgaggt gtccgccgag caaaccgtgg
     3121 acaactgggc gctggaaact ttgcgtttcg tggaggatct cgatcaacac gtcaagtacg
     3181 atcccacttc gattccggct tacacgaaag agggttttga acgtgttcag aaactgaa
//
LOCUS       DASQUQ010000027         4942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_908302, whole genome shotgun sequence.
ACCESSION   DASQUQ010000027 DASQUQ010000000
VERSION     DASQUQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908302"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VE486_00715"
     CDS             complement(<1..277)
                     /locus_tag="VE486_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37639.1"
                     /translation="MDAELQKLVESGRLTAKGAEHLEQLKPGTFCLHKSWGFGRVVEW
                     NLLLNQIVIDFAGKKSHPMQIQYAAENLTPLASDHFLARKANDLASIK"
     gene            376..1311
                     /locus_tag="VE486_00720"
     CDS             376..1311
                     /locus_tag="VE486_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZA37640.1"
                     /translation="MTTSIWFWIAFHIGVFIALTIDLVTFKRRDRALSMRAAVRRSAL
                     WVLVSLGFNVLVWRLKGPQHGLDFLTAYLIEYSLSADNIFVFVLIFAHFRVPPRAQHR
                     VLVWGIIGALIMRGTMILAGIALVQRFHFILYIFGVFLLFTALRMLLGKHPAPDVTEG
                     WVMRTCRRLFPISSEFHGEHFKVRVDGRWMLTPLALTMIVIEVTDLMFAVDSIPAVFA
                     ITRDPFIVYTSNICAILGLRSLYFLLAGLMERFIYLRTGLAFVLGFVGIKMIVADYFH
                     LPSGVSLAVIVVILAITVTTSMIVTRKRAAAIDRK"
     gene            1360..1764
                     /locus_tag="VE486_00725"
     CDS             1360..1764
                     /locus_tag="VE486_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HZA37641.1"
                     /translation="MKTLLALAVIACFAISALADIQDPPSNDYGPTRKLGRGLSNFFI
                     APAEVFVTVTTINTYEGNSAAAGYGVVRGVGRSAARHVAGLLEILTFPFPAWRESYYP
                     ILPSDIAYIHAGYSEFPPELGNESKYPYVRDY"
     gene            complement(1819..2298)
                     /locus_tag="VE486_00730"
     CDS             complement(1819..2298)
                     /locus_tag="VE486_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37642.1"
                     /translation="MKKFPIILLFLAVSVLSPISAWAQGAEFPGVQKAMPPQEYDAAG
                     LQKLTPDERARLDEFIRRFVSSSNQVAAEKAVDRAVKERKVTPPEIIQSRIVGPFTGF
                     TGSTAFTLENGQRWRQAQPDSRYFPKVESPPVIIVKGQIGYRMYIAGGGDIRVSRVR"
     gene            2400..3491
                     /locus_tag="VE486_00735"
     CDS             2400..3491
                     /locus_tag="VE486_00735"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HZA37643.1"
                     /translation="MRIVDLKIVDIAFGGKGVARENGKAVFVPYTIEGETVSAEIVRE
                     KKQFAEASLVEVKESSPHRVSPECPYFARCGGCAYQHIDYEHQLATKWHQVRDVLQRI
                     GKLRDIPIRPIIPSPEQYGYRNRITVHAQDGVIGFFRRDSHRLIDIERCPISRDEVNR
                     ALAALRARPQVRDGHYTLRASSRPRIFSQTNDTVAIALRDLIVQLVPANQGLLIDVYC
                     GAGFFAKALLDKFEGVIGIDWDRFAIETARQNATEKETYIAGDVDEELTRANLTALSS
                     QVSGSNISVSSATTLIVDPPATGLTETVRKTIFDLAPATLIYVSCNPATLARDLKELQ
                     EKFTINSVTPLDMFPQTAEIEVVASASYR"
     gene            4026..>4942
                     /locus_tag="VE486_00740"
     CDS             4026..>4942
                     /locus_tag="VE486_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37644.1"
                     /translation="MAEIISAGVHRPRNVDWKRAAALLYGDWGTSKAYVLGLAFVAAG
                     FASFPIILAVCVVTAVVGFNYIIICAHFPDGGGVYSAARQQSRFLASVGALLLIANFI
                     VTAALSGWAGATYLGVPTRYASVAAVAIILVLGAINYFGPKHSGSVSIWLAVPAVIVV
                     VATVAFSARYLNVTHLEPRHTDLGTVWVQFVGVILALSGVEAIANLTGVMKLDANATP
                     SAPKVTRTATKAILPVAIEVVAGTALLGWAMLSLPKSYAPELTAHKEDMLRFLGQHYA
                     TAVGGVWFGDGFSIFVGAVFGLLLISAVNT"
BASE COUNT          992 a         1371 c         1376 g         1203 t
ORIGIN      
        1 ttttgatcga tgccagatcg ttcgccttgc gtgccagaaa atggtccgag gcgagcggcg
       61 taagattttc cgccgcgtat tgaatttgca tgggatggga tttctttccc gcaaaatcga
      121 tcacgatctg gttgagcagc aggttccact cgaccacccg gccgaacccc caactcttat
      181 gcaagcaaaa cgtgcctggt ttcaactgtt cgaggtgctc ggcgcctttc gctgtaagtc
      241 ttcccgattc gacaagtttt tgcagttcgg cgtccattaa gcggactcgt gagtccgatg
      301 tgacctatag gtcgtatgga cttctcttgc aatttgcgat tgcgacacgg ctcgcggcat
      361 gaaaacctcg cagcggtgac gacgagtatc tggttttgga tcgcctttca cataggcgtt
      421 tttatcgcgc tgacgatcga cctcgtcacc ttcaagcggc gcgatcgcgc attgagcatg
      481 cgtgcggcgg tgcggcggag cgccttatgg gtgctcgtgt cgcttggttt taacgtcctg
      541 gtgtggcgtc tcaaagggcc acaacacggg ctcgattttc ttaccgctta cctcatcgag
      601 tattcactca gcgccgataa catttttgtg ttcgtgttga ttttcgccca ttttcgcgtt
      661 cctccccgcg cccagcaccg cgttttggta tggggaatca tcggcgcgct catcatgcgc
      721 gggacgatga tcctggccgg tatcgcgctg gtgcagcgat ttcatttcat tctttatatt
      781 tttggcgtgt tccttctctt cactgcattg cgaatgttgc ttggaaaaca tccagcaccc
      841 gatgttaccg agggttgggt gatgcggacg tgccgacgtt tatttccaat ctcgtccgaa
      901 tttcatggcg aacattttaa agtgcgcgtc gatggccgct ggatgttgac cccgcttgcc
      961 ctgactatga ttgtgatcga ggtcacggac ctgatgtttg cggtcgattc gattccggcg
     1021 gttttcgcca tcacgcgtga cccgttcatt gtctatactt ccaatatttg cgcgattctg
     1081 ggtttacgat ctctctactt tctgctggcc ggcttgatgg agcgcttcat ttatctgcgg
     1141 acgggccttg ctttcgtcct cggttttgtc gggatcaaga tgattgtcgc agattacttc
     1201 cacctgccga gcggtgtgtc tctcgctgtg atcgttgtta ttctcgccat cacggtcact
     1261 acctcgatga tcgtgactcg gaaacgggcc gcggctattg accgaaaata aaatgttgtt
     1321 gcatttgctg tccctaatta tttaactatc gcagcgccta tgaaaaccct cctagccctc
     1381 gcagtcatcg cttgttttgc catcagtgcc ctggccgata ttcaggaccc gccgtcgaac
     1441 gattatggcc cgacgcgcaa actcggtcgc ggtctctcga attttttcat cgctcctgcg
     1501 gaagtttttg tgacggtgac aacgatcaac acgtatgagg gaaattccgc cgccgccgga
     1561 tacggtgtcg tgcgtggcgt gggccgctcg gccgcccggc acgtggctgg cctgcttgag
     1621 atactgacgt ttccctttcc ggcatggcgc gaaagctatt acccgatcct gcccagcgat
     1681 attgcataca ttcacgctgg ctattcggag tttccgcccg agctcggcaa cgaatcaaaa
     1741 tatccttacg tgcgcgatta ctagcaacga gctcctgctg gaagtggagc tggttcgaac
     1801 tgaattaaca ggcgctgtct agcggacgcg agaaacccga atatctccgc ctccggcgat
     1861 gtacatgcgg tagccaatct gccctttaac aatgatcacc ggcggcgatt cgacctttgg
     1921 aaagtaacgg ctgtcgggct gcgcttgcct ccatcgttga ccgttctcca acgtaaaagc
     1981 agtgctgcca gtaaatccgg tgaaagggcc gacgattcgg ctctgaatga tttcaggcgg
     2041 cgtcacctta cgttccttta ccgcgcgatc gactgccttc tccgcagcca cctggttact
     2101 cgaagaaacg aaacgccgga tgaattcatc caatcgcgcg cgctcatcgg gcgtcagttt
     2161 ttgcaagcct gccgcatcat attcctgcgg cggcatcgcc ttttgcacac ctggaaattc
     2221 ggcgccctgc gcccaggcgg aaattggaga gagaacagac acggcgagaa acaacaggat
     2281 gatcggaaat tttttcatac tcggatatta gcaaacactg ctagtcgact aagagagcca
     2341 cggaaagtca aagggattcg ctcgcctgtg ccacactcct gaatgaggat gttaatgata
     2401 tgcggattgt cgatctaaaa atcgtggaca tcgctttcgg tggcaagggc gtggcccgtg
     2461 aaaacggcaa agcggtattc gttccttaca ccatcgaggg cgagaccgtt tcggccgaaa
     2521 tcgtgcgcga gaaaaagcaa ttcgcggaag cgagccttgt cgaggttaag gaaagttcgc
     2581 cgcatcgcgt tagtccggag tgcccctact ttgcccgttg cggcggctgc gcctatcaac
     2641 acatcgatta cgaacatcag cttgcgacca aatggcacca ggtccgcgat gtcttgcaac
     2701 gcattggcaa actcagggat attccgatcc gaccaatcat cccatcgccc gaacagtacg
     2761 ggtatcgcaa tcggatcacc gttcacgcgc aggatggggt aatcggcttt tttcggcgcg
     2821 actcgcaccg tttgatcgac atcgagcgtt gcccaatttc gcgcgacgag gtcaatcgcg
     2881 cgctggcggc attacgggcg cgacctcaag tgcgcgatgg gcattacaca ctgcgcgcct
     2941 catcgcgtcc gcggattttt tcgcaaacga acgacacggt ggcaattgcg ttgcgcgatt
     3001 tgattgttca gcttgttccc gcgaatcagg gactcctgat cgacgtctat tgcggcgcgg
     3061 gatttttcgc caaagcgttg ctcgacaaat ttgagggagt catcggaatt gactgggacc
     3121 gattcgcaat cgagaccgca aggcaaaacg cgactgagaa ggaaacttac atcgcaggcg
     3181 atgtggacga agaactcacc cgcgcaaact tgactgcttt aagcagtcaa gtttcaggga
     3241 gtaacatcag cgtttcgtcg gccaccacgc tgattgtcga tccgcccgcc actggcctga
     3301 ctgaaaccgt gcgcaaaacc attttcgatc ttgccccggc aacgctcatt tacgtctcgt
     3361 gcaatcccgc gacgctggca cgggacttga aagaattgca ggagaaattc acgatcaatt
     3421 ccgtcacgcc gctcgatatg tttccacaga ccgccgagat cgaagtagtg gcatcggcat
     3481 cctaccgatg aacatgcgct gcagctgcac gacgatgcgc tacgtaatgg cgcctgagag
     3541 ccctcacctc aatcctctcc ctgagggaga ggaggacgcg aagcgccagg tgagggttgg
     3601 ttcatttcaa ggcgcgctgg aagtgcatcc cccaagaaag agcttgccca gtgaacaatc
     3661 gcgcaatgga aagtctccgc tcatagcgac ggccgagatt atttcagcag ctctccatcg
     3721 gtcgcgaaac gttgattgga agcgcgccgc cgcgcggctt tacggcgatt agggcacgcg
     3781 caagtgctac gtggtcggtc tggcgtccca tgctgccgga atcaattcca caagaccacg
     3841 gaaagacttc gaaacgggcc gcggggtccc cgaatggcgt ggtcgaattg ggcgcgcgtg
     3901 tcgaggtttg attccggtac aactcgtcgc tccatcaatc caaacaattc gtagtcaaac
     3961 cgggcacggt tgagaaagag cttgccagca agcgctggcc caattgaaag ttacggctca
     4021 tagcgatggc cgaaattatt tccgcaggtg tccatcggcc gcgcaacgtg gattggaagc
     4081 gcgccgccgc gctgctttac ggcgattggg gtacgagcaa agcctacgtc ctcgggctgg
     4141 cctttgtcgc ggccggcttc gcatcgttcc cgatcattct cgccgtgtgc gtggtcacgg
     4201 cagtcgtcgg cttcaattac atcatcatct gcgctcattt ccctgatggg ggcggcgttt
     4261 attctgccgc gcggcagcag agccgctttc tggcgtcggt gggcgcgctt ttgcttatcg
     4321 cgaatttcat cgttaccgcg gctctgagcg ggtgggcggg cgccacttat cttggtgttc
     4381 caactcgtta cgcctcggtg gcagcggtgg caatcattct ggtcctgggg gcaatcaatt
     4441 acttcggtcc aaaacacagt ggcagcgtct caatctggct ggctgtgccg gctgtaatcg
     4501 ttgtcgtggc aacagtcgct tttagcgcgc ggtatttgaa tgtgacgcat cttgagccgc
     4561 gtcacaccga tttggggacc gtttgggtgc agtttgtcgg agtgatcctg gctttgagcg
     4621 gtgtggaggc aatcgccaat ctcactggcg tgatgaaact ggatgcaaac gccacgccga
     4681 gcgcgccgaa agtcacacgc accgctacca aagccatttt gccagtagcg atcgaagtgg
     4741 tcgccggaac cgcgttgctc ggctgggcga tgctttccct gccgaaatcg tacgcgccgg
     4801 aattaacggc gcataaggag gacatgctgc gttttctcgg acagcattat gcaactgcgg
     4861 ttgggggcgt gtggttcggt gatggatttt ctattttcgt cggcgccgtc ttcggtcttc
     4921 tgctcatcag tgccgtcaac ac
//
LOCUS       DASQUQ010000028        15833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_816447, whole genome shotgun sequence.
ACCESSION   DASQUQ010000028 DASQUQ010000000
VERSION     DASQUQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 15833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15833
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816447"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /gene="ftsA"
                     /locus_tag="VE486_00745"
     CDS             complement(<1..1142)
                     /gene="ftsA"
                     /locus_tag="VE486_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZA37645.1"
                     /translation="MASNNLIIGLEIGTSKICVVVGESRHDGTVKILGVGQTPSRGVR
                     KGEIVDFETAMKCVHEAVVDAEQKSDVMIRSVYVAVAGSHLQSFNNRGCVMLPEDRDE
                     IDEQDVEDVRISAREVSLPAQNAFLHSIIHHYHVDGQDGVLNPIGMLGQKLEADFHII
                     HGVRTRIQNTIRCVKELPLDVEDVVFGGLASAQVVLTQHQKDLGALVIDIGGGTTDYI
                     LYTEGAVRQSGCLAIGGDHITNDISMGLRIPMARAEKLKTDEGSCILGNCLPGEVISL
                     KDDSGFAGKEIERETLNTIIHLRLRETFDLLKRRLDEEPFINYLGAGIFITGGCSLLN
                     GIDHLAEEIFEMPAHVAHAQTASGVTSAVENPQFSTAIGLIKYAQAV"
     gene            complement(1145..2218)
                     /locus_tag="VE486_00750"
     CDS             complement(1145..2218)
                     /locus_tag="VE486_00750"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZA37646.1"
                     /translation="MEFVSKTSRSRNRRRAPNARRWRQQHLLDVKVRSRRATQHRVRK
                     VLAVLSRVLLVVGLCAAVYVGGREAARRLFFENRDYQLKTIELQTDGTLQREQVLRAA
                     DLREGENIFRVNLARVRDRIQDLPQVDDVEVVRKLPGEIDIRVVERKPIAWITSEKEI
                     SDPFVSDAAFLVDARGVLMKQKKLLPEYLGLPLILGCSSESLDAGKVVESPEAKTALE
                     LLRLSTRSFMQTRFQIREIDVSQSYCLLVTDKNRSRVTFGLNDLEAQLQRLEQFLVYC
                     DKSKQELETLNLLVQRNIPVTFTDTPTEIINATIEPTVEPRILKAIPVDSPAKAGSPP
                     PKTRAGAAPVAKAIPIQRKRKNQ"
     gene            complement(2221..3156)
                     /locus_tag="VE486_00755"
     CDS             complement(2221..3156)
                     /locus_tag="VE486_00755"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZA37647.1"
                     /translation="MVISANLPKKIAVLMGGPSAEREVSLATGRGISKALRSPGADVV
                     EVDVRDANFELPRDVEFAFIALHGTFGEDGQVQQILEERGVPYTGEGVNGSRLAFDKI
                     RSKEKFREHGVSTPEWETIGSHERPAMQLPIVVKAPRQGSTVGVYIVKKTEELEPAIA
                     NAAKYDRELLIEKFVAGRELTIGILGDQALPILEIIPKGGFYDFTNKYPFLNPQAGGA
                     AQHICPARIEPEKTKEIQELALRAFRALGLQVYSRVDVILSQTGEPFVLEINTIPGMT
                     EASLLPEAAAAAGISYADLCRRVITLSRARIEGAS"
     gene            complement(3372..5666)
                     /gene="murC"
                     /locus_tag="VE486_00760"
     CDS             complement(3372..5666)
                     /gene="murC"
                     /locus_tag="VE486_00760"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZA37648.1"
                     /translation="MKPVSELDLPKFLTCENHRVHLIGVAGSGMSGIAALLLELGHEV
                     TGSDKVSTLETDRLQRRGLRFHGQHRAADASNAELIVFSSAIKSDNPILASARDSGKP
                     VVRRAQALAAIMCGKRGILIAGMHGKTTTSAMTAHVLREGGLHPSHYVGAEIPILGTN
                     AHWDPRGEYFVAEGDESDGTLPCFHPEHSLILNIEEEHLDFYADIAAIEDAFAQLIGQ
                     TTGTVFYNIDDANAARSCAKRKSAVSFGFSEKADYRATNLELRDFASAFCVYRKGQQL
                     GEAVLNVPGQHNVQNAVGVIALATEVGISFDKIAASLRKFEHARRRFEIKYQSDRFLL
                     VDDYAHHPTEIRATLKSATSIGRKRVLAMFQPHRYSRTKALRKEFGRAFDDADRVVVT
                     DVYPASEAPIPGISGQTIADEIATHGHRGVSYQPFMERLHRDVGNMLDSGDLVLSLGA
                     GNIHEQLSLLAADLVIAEKLIQIVGGEGDVRLYEPLSKHTTLRVGGPAQFWVEPRDEK
                     AFSKLIRFCRQEDLSLFVIGRGSNLLVRDGGIRGVVVHPRGGDFDKVEVNGNEITAGA
                     GAKLKEVAYAGKAAGLGGLEWMEGIPGAVGGGLRMNAGAMGAQTFENVVRVRYLDANG
                     EAHTKTRDELEVHYRSFPLLESNFAISAVFRGEPAPTEEIVRNLRASQEKRRTSQPAA
                     KSAGCIFKNPDSCSAGKLVDELGLKNSRVGNARVSEVHGNFIVNDGGATAADMLELIE
                     KIKGVARAKRRIELETEVQIVGVD"
     gene            complement(5650..6747)
                     /gene="murG"
                     /locus_tag="VE486_00765"
     CDS             complement(5650..6747)
                     /gene="murG"
                     /locus_tag="VE486_00765"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZA37649.1"
                     /translation="MNTVIACGGTGGHLFPGIAVAEVLRDRGHKVMLLVSEKDIDALA
                     LSTHRSFQFEKLPTIGLPSPFSPAILGFIRRFYESLSLCRSIYGKFKPQVVLGMGGFT
                     STAPVLAGRMRGISTFIHESNAVPGKANRLTARIVRAVMLGFKECAPFFPKAHTEITG
                     TPIRTELKPLDRQIARQKLGLRDDLTTLLVMGGSQGASGINQAMIKALPFFQGAPLQV
                     IHLSGARDERLVADNYRRENISAYVAAFHHRMEEVYSAANFVVGRAGAASLAEFAAFS
                     LPGILIPFPYAADDHQRRNAEIYARAEAAILLRESELSGELLARKIRELIENPELIER
                     MSANCSRLGPKDAAGCVVTTMEKYTTHEARL"
     gene            complement(6804..7946)
                     /gene="ftsW"
                     /locus_tag="VE486_00770"
     CDS             complement(6804..7946)
                     /gene="ftsW"
                     /locus_tag="VE486_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZA37650.1"
                     /translation="MHRKSAYILFLAVLALLVIGIVMLFSTSAFARNSHGDVYFFIRR
                     QAIWFGIGLFVCTLAALVDYHFWQRTWWLWFALAVIVLALCYVPHIGMRINGSRRWIG
                     VGPVTFQPSELAKLAAVFFLAAWFTRHEKAGGNLLFGFVLPLAIIGVLAGLVLGEVDL
                     GTAALLGLTTFVVMFIAGANLLWLGAVSFVSLGALLVVATQISERMGRLSAFLNPQNF
                     KEDAGLQQMQALIAWGSGGMEGLGLGNGRQKMLYLPYAHTDFIFPIIGEELGLRFSLL
                     VVFLFVVIIVCGIMIALHAKDRFGLLLGSGVVSLLALQAAMNIGVTTSLLPNKGLPLP
                     FISYGGSNLAACMFGVGLLLNIYRQGILEPPHKKRVTMQARMTPRI"
     gene            complement(8100..8687)
                     /locus_tag="VE486_00775"
     CDS             complement(8100..8687)
                     /locus_tag="VE486_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZA37651.1"
                     /translation="MKLKIPRLLRRKKLRAATARRPASGSAEMDYGEMSEPNMKLSRA
                     LLIVLLLHVVAVSGIIAFNAIKTRQSSLAPVPPGNTENTPAARSAGSTINSEPSNPKS
                     SVIPRDEAKPSRSPSKSMTKDEHPKSSEAEKAPVSSGKFYTVAKGDNPVTIAKKLNVS
                     YGDLTALNHIEDPRKLQIGQKLLIPKTTKPKKNEK"
     gene            complement(8684..10042)
                     /gene="murD"
                     /locus_tag="VE486_00780"
     CDS             complement(8684..10042)
                     /gene="murD"
                     /locus_tag="VE486_00780"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZA37652.1"
                     /translation="MRYKDQNIAVLGAGLSGAAAALLLRSEGARVTVLDSADKKNLLK
                     STLDNLRTQGVTVICGRAADEDSTPYQMAVLSPGIDPASALARNFSSRNSAGRTDSSG
                     GEVEMIGELELGWRSCEIPVIAVTGTNGKTTTTELLAQVLNACGQRTIACGNIGKPLS
                     EVAREKKPFDVLTVEVSSFQLETIQTFRPSISLWLNFAPDHLDRYRSVADYRAAKLRV
                     FENQTADDIAVVNAIEKIPGIRARTITFSAYTDQADFRLSEGAIIYDGQAVLRLSDTK
                     LRGLHNIENLMATLAVGMARGLSFSEMAPPLRRYEPQPHRCEFVREVGGVGYINDSKA
                     TNLDALEKALRAQTKPVILIAGGKDKGFTFDPLRSLVKEKVRSTILIGEMAGSIRQSW
                     NSAAKSEIANSLADAVERAHRIAKPGEVVLFSPGTSSFDMFKSYADRGDQFRALVRAL
                     PK"
     gene            complement(10046..11146)
                     /gene="mraY"
                     /locus_tag="VE486_00785"
     CDS             complement(10046..11146)
                     /gene="mraY"
                     /locus_tag="VE486_00785"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZA37653.1"
                     /translation="MIYYLHQLSEHFIGFHVFLYVTFRAIAAAVTAFLVTLIFGNFII
                     RKLTALKMGQPIRGAAEVRHLAELHGGKQGTPTMGGVLVIGSVFVSSLIWARPDNRFV
                     WLALFSMIYLGALGFVDDYLKVTKKKSEGISGRLKLVFQIALAAIVTTVFLSSPLLEV
                     QARSLYVPFFKAPVIVNMGWFTFVFFALVIVGSSNAVNLTDGLDGLAIGCTITVAFAY
                     ALLSYAAGNFRIAEYLQVPFYPFAGELTVVCSALVGAGLGFLWFNCHPARVFMGDTGS
                     LAIGGMIGVVAICCKQEFLLIVVGGVFVIEAVSVILQVISFKMTGRRIFVMSPLHHHF
                     ELTGWKENIVIVRFWILSIIFALLGLATLKLR"
     gene            complement(11143..12537)
                     /gene="murF"
                     /locus_tag="VE486_00790"
     CDS             complement(11143..12537)
                     /gene="murF"
                     /locus_tag="VE486_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZA37654.1"
                     /translation="MDPLPISQIAKFAGASLSSGRGTIVIDKIRTDSRTLKGGELFVA
                     LRGENFDGHNFVEAAGKANAAGAIVESNWKGKLPETFALIRAEEPLQAYQELAANYRR
                     SLSLKVVAITGSNGKTSTKDFAAAVLARRFRVTKTEGNFNNHVGLPRTILEATSHDEV
                     AVWEIGMNHPGEIAALAPLAMPDVAIITNIGVAHIEFMGSREAIAAEKGTLAEAVSAE
                     GTVILNADDPFTKSIATRARGKVLLAGTTAGTIRASEISQSESGTDFTILEGAHRCRA
                     QLPVPGLHMVQNALLAVAAGRVFGLSLEDCAAGLVAAPLTKARLQIKEIQGVHFLDDS
                     YNANPDSMKAALRTLVELDADGKRIAVLGEMRELGSESEHGHREVGETAAALKVDHLI
                     TIGNVAATIAQAAEHAGLEKSGAVNSTTEAAELLGEIVEPGDLVLIKGSRAARTEEVI
                     EHFRLRHSSFVISP"
     gene            complement(12540..14018)
                     /locus_tag="VE486_00795"
     CDS             complement(12540..14018)
                     /locus_tag="VE486_00795"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZA37655.1"
                     /translation="MQLKTLVAAIPVRHVIGTLDRPVESIAYDSRRMQRNGMFVALRG
                     EKTDGHEFIGQAIEKGASVIVTEREEKTPRVTCLVVENTRAALADLSATFYGHPARKL
                     KLAAVTGTNGKTTTTFLIKYICEKAGLRCGLIGTVRYEIGERVLPAIRTTPESLDLQE
                     LLAQIANAGCRAAAMEVSSHALAQDRTRGLEWDVAVFTNLTQDHLDFHGTMESYFDSK
                     AKLFAQLGSQERKLKPVAIVNIDDRYGEQLLGKIDKRVAVITCGMSARADFRASNYRV
                     EFSGTSYQLDARGKSYLVRVPLIGRFNVANSIAALAAANALGISLRDAVLSLGKSPQV
                     PGRLEIVPAKRQFQVFVDYAHTPDALQNVLKTLHELEPSRLIVVFGCGGNRDRQKRPL
                     MGEIVDRNADYAIITSDNPRKEDPGAIIDQIEKGFRSNRYEKMIDRTEAIGRAVSLAQ
                     PRDIILIAGKGHENYQEFADHTVPFDDIQVARRAIEDHPMEF"
     gene            complement(14077..>15833)
                     /locus_tag="VE486_00800"
     CDS             complement(14077..>15833)
                     /locus_tag="VE486_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZA37656.1"
                     /translation="LFSFRLIYLQVIKHDEYAALAAEKHVYKQIIYAERGSILDANSE
                     VLAHNIPVETVVADATLFNDREAVVDLVSRELSIPVEQVAEKLSSERRYIVIKREVPE
                     TASRSLREKLRAKNLRGIYFEHDSSRIYPNGSMLCHVIGFTDFDHHGIQGVEASMEEY
                     LHGQDGYRFIEHNRAGQEIVPYRGQEHPPRNGCQIHLTVDLSLQNIVENEIDAAMAEY
                     SPQKATIILMRPQTGEILAMANRPNFDLNLRSEAKPEEMKNRAIIDMMEPGSTFKIVA
                     AAAALNERKLRSDSTVFCENGLWNFNGTALHDHRAFSYLSVRDILIRSSNIGAAKLAI
                     SVGEQKFYEYVRRFGFGERTGIELPGEINGLIRPPQKWSKISITRIPMGHEIGVTPLQ
                     MTAAMATIANGGKLMTPRIVKSITTSDGKSISSLSPVTARQVISPETARQIGDALRGV
                     VSDRGTAAAAAVPGFTIAGKTGTAQKVDPRGGYEHGKYVVSFAGYLPAEHPEFVGLVV
                     LDDAQTSKPELNYGGLIAGPIFSRVAEKAAQYLDLRPQEQIRKAIPVERVQKAVPAER
                     GRKTVPADRVALTNASRP"
BASE COUNT         3507 a         4562 c         4242 g         3522 t
ORIGIN      
        1 acggcttgcg cgtacttgat cagcccgatt gcagttgaga actgtggatt ctccaccgcg
       61 gacgtcacac cggacgcggt ttgtgcgtgg gccacatgcg cgggcatttc aaaaatttct
      121 tcagccagat gatcgatgcc gttaagaaga ctgcatccac cggtgatgaa aatgccggcc
      181 ccgaggtagt tgatgaatgg ttcctcatcg agcctgcgtt tgaggaggtc gaaagtttcc
      241 cgcaagcgca ggtgaatgat cgtgttcagc gtctcgcgct cgatctcttt cccggcaaag
      301 ccggaatcgt cctttagtga aatcacttcg ccgggaagac aattgccgag gatgcagctc
      361 ccttcgtccg tttttaattt ctccgcacgc gccatcggga tgcgcaatcc catcgaaata
      421 tcatttgtga tatggtcccc gccgatcgcc aggcagccgc tttgcctcac tgcgccctca
      481 gtatataaaa tatagtcggt tgtcccgccg ccaatatcga tcaccagcgc gccaagatct
      541 ttttggtgct gagtgagaac gacctgggca gaagcaagcc cgccgaagac aacgtcctcg
      601 acgtcgagtg gcaactcttt cacgcaccgg atcgtattct gaatgcgcgt ccggacaccg
      661 tggatgatgt ggaaatcggc ttccaatttt tgcccaagca tcccgatcgg attcaacact
      721 ccgtcctgcc catcgacgtg ataatggtga atgatggaat gcaggaaagc attttgcgca
      781 ggaaggctca cctcgcgggc gctgattctt acatcctcca catcctgttc gtcgatctca
      841 tcgcgatcct ccggcagcat tacgcagccg cgattattga agctttgaag gtgggacccg
      901 gctacggcaa cgtaaacgct tcggatcatg acgtcgcttt tttgctcagc atcgaccact
      961 gcttcgtgaa cgcatttcat tgcggtctca aaatcaacga tctcaccttt gcggacgccg
     1021 cgcgatggcg tctggccaac gccgaggatt ttaaccgtgc catcatgccg gctttcgccg
     1081 acgacgacac agattttgga ggtgccaatc tccaggccaa tgatcaaatt attgcttgcc
     1141 atcgttattg gttctttctc ttccgttgga tcggaatagc tttggcgaca ggcgccgcgc
     1201 cggcgcgagt tttcggcggc ggcgatccag cttttgctgg tgaatccacc ggaattgctt
     1261 tcaaaattcg tggctcaacg gtgggctcga ttgtggcatt gatgatttcc gttggagtat
     1321 cggtgaaggt caccgggata ttccgttgca cgagcaggtt caaagtctcc agctcctgct
     1381 ttgacttgtc acaatagaca agaaattgtt ccagcctctg taattgtgcc tcgagatcat
     1441 ttagcccaaa cgtgacgcgt gagcgatttt tgtccgtcac gagcagacaa taactttggg
     1501 aaacatcgat ctcgcgaatc tgaaagcgcg tctgcatgaa gctgcgcgtg ctcaagcgca
     1561 aaagctccag cgccgttttg gcttcgggcg attcgacgac ttttcccgca tcgagcgatt
     1621 cgctcgagca tcccaaaatg agcggcaatc ccagatattc cggcaacaac ttcttttgtt
     1681 tcattaaaac accccgcgcg tccacaagaa aagcagcatc cgagacaaat ggatcggaaa
     1741 tctctttctc acttgtaatc cacgcgatcg gctttcgttc caccacgcgg atgtcgatct
     1801 ccccgggcag cttgcgcacc acttccacat catcgacctg ggggaggtcc tgaatgcgat
     1861 cacgcacgcg cgccaggttc accctgaaaa tgttttcgcc ctcacgcagg tccgccgccc
     1921 ttaaaacctg ctcccgctgt agcgtgccat cggtctgcag ttcaattgtc ttgagctgat
     1981 aatcccgatt ctcgaagaaa aggcgcctcg cggcttcgcg ccctcccaca taaacagcag
     2041 cgcagagccc caccaccaat agcacccggg agagaacagc cagaaccttg cggacgcgat
     2101 gttgcgtcgc tctgcgggag cgaaccttta cgtcgagcag atgttgctgg cgccaccgac
     2161 gcgcattggg cgcgcgccgg cgattacgcg aacgggaggt tttcgagacg aactccatct
     2221 tcagcttgcc ccctcaattc tggcgcgtga aagcgtgata acgcgccggc aaagatccgc
     2281 atagctgatg cccgcggccg cagcggcttc gggcaggaga ctcgcctcgg tcattcccgg
     2341 aatcgtattg atctccaata caaacggctc gccggtctgc gataaaatga cgtccacccg
     2401 cgaataaact tgaaggccga gggcgcgaaa cgcgcgcagc gccagctctt gaatctcttt
     2461 cgtcttttcc ggttcaattc tggccgggca aatatgctgc gcggcgccgc cagcctgcgg
     2521 attgagaaag ggatacttgt tagtgaaatc gtagaagccg cccttgggga ttatctcgag
     2581 aatcggcaag gcctgatcac cgagaatacc aatcgtcaat tcccggcccg ccacaaattt
     2641 ttctatcagc aattcacggt catacttcgc cgcgttggcg atcgccggct cgagttcctc
     2701 cgtcttttta acgatgtaaa caccaacggt tgatccctgt cgcggcgcct taaccacaat
     2761 gggcaattgc atggccgggc gctcatgcga tccgatcgtt tcccattccg gagtgctgac
     2821 accgtgttca cggaattttt ccttcgaccg gatcttatcg aaagccagcc ggctgccatt
     2881 gactccttca cccgtgtaag ggacgccgcg ctcttccaga atttgctgga cctggccgtc
     2941 ttcgccaaaa gttccgtgca acgcgatgaa tgcaaactcg acatcgcgcg gcaactcgaa
     3001 gttcgcatct cggacatcga cctcaacaac atccgcgccg ggtgagcgca atgcctttga
     3061 gattccccga cccgtcgcca gcgaaacttc acgctcggcg ctcgggccgc ccattaggac
     3121 cgcgattttt tttggtaaat tagcagagat caccatggcg ttatcctagc atgtgaacgc
     3181 gagttgattg gggtagcgca ggcgtctcgc gtgttggttc cggcgtcgcg ccgaaacgga
     3241 ctttccgaaa gattcagaac gcgggagaag attccactta tagaaaagtt cgcgatggcg
     3301 aagacgccat cgccaacacg cgagacgcgt gcgctaccca gactccgtat ttcaacaatg
     3361 ttcatctctc gttaatccac tcccacgatt tgtacttctg tttccaattc gatcctgcgc
     3421 ttggcacggg ccacgccttt gatcttttca attaactcga gcatgtccgc cgccgtcgcg
     3481 cctccgtcat tcacaatgaa attgccgtgc acttccgaca cgcgtgcgtt tccaacgcgc
     3541 gaattcttta gtcccaattc atcaaccagc tttcccgccg agcacgaatc gggattcttg
     3601 aaaatgcagc ctgcgctctt cgccgccggc tgcgaagtgc ggcgtttttc ctgcgatgcc
     3661 cgcagatttc ggacgatctc ctcagttggc gcgggttctc cgcgaaaaac cgctgagatc
     3721 gcaaaattac tctcgagcaa cggaaaactt ctgtaatgca cttccagctc atcgcgtgtt
     3781 ttggtgtggg cctcgccatt tgcatcgaga taacgaacgc gcacgacgtt ttcaaaagtt
     3841 tgcgcgccca tcgctccagc attcatgcgc agaccgccac cgactgcgcc cgggatcccc
     3901 tccatccact ccagtccacc gaggccggca gccttccccg cgtaagcaac ctctttcaat
     3961 tttgcgccgg cgccggccgt gatttcatta ccattgacct caaccttgtc gaaatcacca
     4021 ccgcgaggat ggaccacaac accgcgaatg ccaccatcgc gcaccagcag atttgatccg
     4081 cgaccaataa caaagagcga aagatcttcc tgccggcaga accggatcaa tttcgaaaac
     4141 gccttctcgt cacgcggctc gacccaaaat tgcgccgggc cgcccacgcg cagcgtggtg
     4201 tgtttcgaga gcggttcata gagcctgacg tccccttccc cgccgacgat ctgtattaat
     4261 ttctcggcaa tcaccaggtc ggccgcgagg agcgataatt gctcgtgaat gttgcccgcg
     4321 cccaggctca aaaccagatc gccagagtcc agcatgttgc cgacatcgcg atggagccgt
     4381 tccatgaatg gctgatagct gacgccgcgg tgaccgtgcg tcgcgatttc atccgcgatc
     4441 gtttgtccgc tgattcccgg aattggcgct tcgctggcgg gataaacatc ggtcacgacc
     4501 acgcggtcag cgtcatcaaa ggcgcggccg aattctttac gcagcgcttt tgtccgcgaa
     4561 taccgatgcg gttgaaacat ggccagcacg cgtttgcgac cgattgatgt tgcgcttttc
     4621 agcgtcgccc ggatctcagt ggggtgatga gcgtagtcgt caacgagcaa aaaccgatcg
     4681 ctctgatact tgatttcgaa gcggcggcgc gcgtgctcga acttgcgcag cgaagcagca
     4741 attttgtcga acgaaattcc aacctcggtg gcgagagcga ttacaccgac agcattctgc
     4801 acgttgtgct ggccgggaac attcaggacc gcttcgccca gttgctggcc ctttcgataa
     4861 acgcaaaagg ccgaagcaaa atcacgcagc tcgaggttag tggcgcggta gtcggccttt
     4921 tccgaaaagc cgaacgacac agcggacttc cgttttgcgc atgatcgcgc ggcgttcgcg
     4981 tcgtcgatat tgtaaaaaac ggtcccggtg gtttgcccga tgagctgcgc aaaagcgtct
     5041 tcaatcgcgg caatatcggc atagaaatcg agatgctctt cctcgatatt taaaatgaga
     5101 gaatgctcgg gatgaaagca gggcagcgtc ccatcgcttt catcgccttc cgcgacaaaa
     5161 tattcgccgc gcggatccca gtgcgcgttt gtcccaagaa tcggaatctc ggcgccaacg
     5221 taatgtgatg gatgcagacc gccttcgcgc aaaacgtgcg cggtcattgc cgaggtggtc
     5281 gtttttccgt gcattcccgc gataagaatg ccgcgctttc cgcacatgat agcggcgagg
     5341 gcttgtgcgc ggcgcacgac cggtttgccc gaatcgcgag cgctcgcgag aattggattg
     5401 tcggacttga tcgccgagga aaatacaatc agctctgcgt tgctggcatc ggccgcgcga
     5461 tgttgcccat ggaaacgtag accgcgccgt tgcagtcgat ccgtctccag agtgctgact
     5521 ttatcagagc cagtcacctc atgcccgagc tcgagcaaaa gcgcggcgat accgctcatc
     5581 ccgctcccgg ccacgccgat aaggtgaacc ctgtgattct cacaagtgag aaactttggc
     5641 agatccagct cagagacggg cttcatgagt cgtatatttt tccatcgtcg taaccacaca
     5701 tccggctgcg tctttcgggc cgagccgcga acaattcgca gacattcgct caatcagctc
     5761 cggattctca attaattccc ggatcttgcg cgcgagcagt tcgccagaga gctccgattc
     5821 cctaaggaga atcgctgcct cggcgcgagc ataaatttcc gcgttgcgcc tctgatgatc
     5881 gtcggcggca tagggaaatg gaattagaat gcctggcaat gaaaaagcgg caaactcagc
     5941 caggctcgcc gcgccagcgc gtccaaccac gaaattagcg gcgctgtaaa cttcctccat
     6001 ccgatgatga aaggcggcga cgtaggcaga aatattttcg cggcgatagt tatctgccac
     6061 cagacgctca tcccgcgctc cggaaagatg aattacctgg agaggtgcac cctggaaaaa
     6121 cggcaacgct ttgatcatgg cctgattgat tccgctggcg ccctggctcc ctcccatcac
     6181 cagtaatgtc gtcagatcat cccgcaggcc gagcttttgg cgcgcgatct gtcgatctaa
     6241 aggtttcagt tcggtccgga tgggcgtgcc ggtaatttca gtatgcgctt ttggaaagaa
     6301 tggggcgcat tccttgaatc cgagcatgac tgcacgcacg atccgagccg ttagccggtt
     6361 cgccttgccc ggaacagcat ttgattcgtg aataaaggtg gaaataccgc gcatcctgcc
     6421 ggccaaaacc ggggcggtgg aagtgaaccc gcccatgccg agaactacct gcggtttaaa
     6481 tttgccgtag atagaacgac aaagcgacag gctctcgtaa aagcggcgaa tgaaaccaag
     6541 aattgccggc gaaaatggcg aaggtaatcc gatggttggc agtttctcga attggaaact
     6601 ccggtgggtg gaaagagcga gcgcgtcaat gtctttttcc gaaacgagca acatgacttt
     6661 gtggccgcga tcgcgcagca cttcggcgac agcaattccg gggaaaagat gtccaccggt
     6721 tcctccgcac gcgatgaccg tattcataag agttgatcct ctccctcccg acccatatga
     6781 cttatatgac tcatgagtcg catttaaatc cgtggtgtca tccgagcttg catggtgacg
     6841 cgttttttat gcggtggttc aagaatgccc tgtcgataaa tattcagcag caagccaact
     6901 ccgaacatac aagcggccag atttgaacct ccataactga taaagggcag cggcaaacct
     6961 ttgtttggaa gcagcgaggt ggtgacgcca atgttcatcg ccgcctgcaa agccagcagg
     7021 gaaacaacgc cactgccaag aagcagacca aaacgatctt tcgcgtgtaa cgcgatcatg
     7081 attccacaaa caatgatgac cacgaacagg aacaccacca gcagactgaa ccggagaccg
     7141 agctcttcgc cgatgattgg aaatatgaaa tcggtgtgcg cataaggcag ataaagcatc
     7201 ttttggcgac cattgcccag gccaaggcct tccattcctc cgctgcccca cgcgatcaag
     7261 gcctgcatct gttgcaaacc cgcatcttcc ttgaaatttt gcggattaag aaaggcggag
     7321 agccgtccca ttcgttccga gatttgtgtc gcaacgacaa gaagagcgcc aaggctgacg
     7381 aacgatacgg cgccaagcca cagcaggttt gcgccggcaa tgaacatgac gacaaaggtc
     7441 gttagtccga gcagagccgc cgttccaaga tcgacttcgc ccagaacaag tccagccagc
     7501 acaccgatta ttgcgagcgg aagcacgaat ccaaaaagca gattgccgcc agctttttcg
     7561 tgccgggtaa accacgcggc gaggaaaaag acggccgcca gttttgccag ctcggatggc
     7621 tgaaatgtaa ccggccccac tccgatccag cggcgcgaac catttattcg catgccgatg
     7681 tgcgggacgt aacaaagcgc cagaacgatt actgcgagcg cgaaccaaag ccaccatgtc
     7741 cgctgccaaa agtgatagtc gaccagcgca gccaaagtgc agacgaaaag tccaatccca
     7801 aaccaaatcg cctgccgcct gataaagaaa taaacgtcgc catggctgtt gcgggcaaac
     7861 gcgctggtgc tgaaaagcat cacgatgccg atgacgagca gcgccagcac agcgaggaaa
     7921 aggatatagg cgcttttacg atgcatgatt agttcgtggc acgggcttcc agcccgtggg
     7981 gccggcgggc atcttgcctc ccggataatg actcgcaggc gagacgtctg ctcgccccac
     8041 aggcaagatg cctgtgccac acgcgtgcgc tacccggatt gaaatcggcg ccatgggtgt
     8101 catttttcgt tcttttttgg cttcgttgtt ttcgggatca aaagcttttg gcctatctgc
     8161 aacttgcgcg ggtcctcgat gtggttgagc gcggtcagat cgccgtaaga gacgtttagt
     8221 tttttggcga tcgtcaccgg attatcgcct ttcgctaccg tataaaattt ccccgaggac
     8281 acgggcgctt tctcggcttc gctcgatttc gggtgctcgt ctttcgtcat cgatttcgaa
     8341 ggtgaacgtg acggtttcgc ctcatcgcgc ggtatcaccg acgatttcgg atttgaaggc
     8401 tctgagttaa tcgtcgatcc cgcgctcctg gcggccggag tattttctgt gtttcccggc
     8461 ggtacgggcg ccaacgaact ctgtcgcgtc ttgatcgcgt tgaatgcgat aattccggag
     8521 acagcaacca cgtgaagaag caaaacgatc agcaacgccc gcgacagctt catgttcggc
     8581 tccgacatct cgccataatc catttccgct gagccgctag cagggcggcg cgcggttgcg
     8641 gcccgaagtt ttttgcgcct gagaagcctg ggaattttca gtttcatttg gggagagctc
     8701 gcaccagggc gcgaaattga tcgccgcgat ccgcgtagct tttgaacatg tcgaaggatg
     8761 atgtgcccgg ggaaaaaagg acgacttcgc cgggtttggc gatccggtgt gcgcgctcaa
     8821 ctgcgtccgc aagcgagttc gcgatttcag atttggccgc gctgttccaa gactgcctga
     8881 tgctcccggc catttcgcct atcaaaattg tcgatctaac cttttctttc acgagagatc
     8941 ggagcggatc aaacgtgaaa cctttgtcct tgccgccagc gatcagaata accggtttgg
     9001 tctgggcgcg gagcgccttt tccagcgcgt cgaggtttgt cgcctttgaa tcgttgatgt
     9061 agccgacgcc accgacttcg cgcacaaatt cgcagcggtg cggttgcggc tcatatcggc
     9121 ggagcggcgg agccatttct gaaaacgaca atccgcgagc catccccacc gcgagcgtcg
     9181 ccatcaaatt ctcaatgtta tgcaagccgc gcagtttcgt atccgacaac cggagcacag
     9241 cctggccatc gtagatgatt gcgccttcgg agaggcgaaa gtccgcttga tcggtgtaag
     9301 cgctaaaggt gatcgtgcgt gcgcgaatcc cggggatctt ctcgattgca ttaacaaccg
     9361 cgatatcgtc cgcggtttga ttttcaaaaa cccgcagctt ggccgcgcga tagtcggcaa
     9421 cggagcgata ccggtcgaga tggtcgggag caaaattaag ccagagactg attgacggcc
     9481 gaaaggtttg aatggtctcc agttgaaatg aacttacttc gaccgtgagc acgtcgaatg
     9541 gtttcttctc acgcgccact tccgaaagcg gtttgccgat gttgccgcaa gcgatggttc
     9601 gttgcccgca ggcgttcaac acctgggcga gcaattcggt cgtcgtcgtt ttaccattcg
     9661 tgccggtaac ggcgatgact ggaatttcgc aggatcgcca cccgagctcg agctctccga
     9721 tcatttcaac ctcgccaccg gacgagtccg ttcgaccggc agagttgcga gaagaaaaat
     9781 tgcgtgcgag tgccgaggcc ggatcgatcc ccggacttaa gacagccatt tggtaagggg
     9841 tcgaatcttc atctgccgcc ctcccgcaga tcaccgtcac tccttgcgtc cgcagattat
     9901 cgagagtcga tttaagcaaa ttcttcttat cggcgctatc gagcaccgtg acccgtgcac
     9961 cctcggagcg aagcagcagc gccgcggcag cgccgcttaa gcctgcacca agaaccgcga
    10021 tattctgatc tttgtaacgc aatttttacc tcagtttcag tgtcgccaga ccaagcaggg
    10081 caaagatgat cgacagaatc caaaaccgga cgatgacgat gttttccttc caaccggtca
    10141 gctcgaaatg gtgatggagc ggggacatga cgaaaatccg ccggcccgtc attttgaagc
    10201 tgatcacctg caaaatcacc gagaccgctt cgatcacaaa aacgccgccc acgacgatca
    10261 ggaggaattc ctgcttacaa cagatcgcga cgacgccgat catgccgccg atggcgagcg
    10321 atcctgtgtc ccccatgaag actctggccg ggtgacagtt gaaccaaaga aagccaagac
    10381 cggcgccgac cagggctgaa cagacaacgg tcagctctcc cgcaaacgga taaaatggga
    10441 cctgtaaata ttcagcgatt cggaagtttc ctgccgcata ggaaaggagc gcatatgcaa
    10501 acgcgaccgt gatggtgcag ccaatcgcga gaccatccag tccatcggtg aggttgaccg
    10561 cattcgagga gcccacaata acgagcgcaa aaaacacgaa cgtaaaccag cccatattga
    10621 cgatgaccgg cgctttaaaa aacgggacat agagcgaacg cgcctgcact tcgagcaacg
    10681 ggctgctgag gaaaacggtg gtgacaatcg ccgccagagc gatttggaaa accagtttga
    10741 gccggccgct gatgccttcg gactttttct ttgtgacctt aaggtaatcg tcaacgaatc
    10801 cgagcgcgcc gagataaatc atgctgaaca aggccagcca aacgaaacga ttatccggac
    10861 gtgcccagat caggctcgat acaaacacgg atccaatgac gagcacgcct cccatggttg
    10921 gcgtgccctg cttgccgccg tgcagttcgg caaggtgacg cacttctgcc gccccgcgaa
    10981 tgggctgccc catcttcagc gcggtcagtt ttcgaataat aaaatttccg aaaatcaacg
    11041 tgaccagaaa agcggtcacg gccgcggcaa tggcgcggaa tgtgacatac aggaaaacat
    11101 ggaagccaat gaagtgttcg ctcagttgat ggaggtagta gatcatggcg aaatgacgaa
    11161 tgacgaatga cgaagccgga aatgctcgat cacttcttcc gtccgtgcag cacggctgcc
    11221 tttgatgaga acgagatcgc ctggctcgac aatttcgcct aacaactcgg cggcttcagt
    11281 tgtcgaatta accgccccac ttttttccaa accggcgtgt tccgcggcct gggcgattgt
    11341 agcggctaca tttccaatcg taattaaatg gtcgaccttt aacgcggccg cggtttcgcc
    11401 gacttcgcga tggccgtgct ccgattcgga accaagttct ctcatttccc ccaggacagc
    11461 gatacgtttt ccgtcggcgt ccagttcaac taatgtgcgc aaagccgctt tcatggaatc
    11521 gggatttgcg ttgtagctgt cgtcgagaaa atgcacgccc tggatctctt tgatttgcaa
    11581 acgcgctttg gtcagtggcg cagcgacgag gccggcggcg caatcctcga gggaaagacc
    11641 aaagacgcgt ccggcggcga ctgcgagcag cgcgttttgc accatgtgca aaccgggcac
    11701 cggcagttgg gcgcggcagc ggtgcgctcc ctccaatatt gtaaaatctg ttccgctctc
    11761 gctctgactg atttcgctgg cccggatcgt ccctgcggtt gtgccggcta ggagcacttt
    11821 gcctcgcgcg cgcgtcgcta tgctcttcgt aaatggatca tccgcattta gaatgaccgt
    11881 cccctcagcg ctgacagctt cggccagtgt gcctttctcc gctgcgatcg cctcgcgcga
    11941 tcccataaac tcgatgtgcg cgacgccaat gtttgtaatg atggcaacgt ccggcatggc
    12001 aagcggcgcc aatgccgcta tttcgccagg atgattcatc ccgatttccc agacagcgac
    12061 ttcatcgtgc gaagtcgcct ccaggattgt tcgaggaaga ccaacatgat tgttaaagtt
    12121 tccttctgtt tttgtgactc gaaaacggcg ggcaaggacc gccgcggcaa aatctttcgt
    12181 actggtcttg ccgttgctcc cggttatcgc gacgaccttg agcgagagcg acctcctgta
    12241 gttggcggcc agctcttggt atgcctgcaa aggttcttcc gcacgaatga gcgcgaaagt
    12301 ctctggaagc ttccccttcc aattggactc cacgatcgcc cccgccgcat tcgcctttcc
    12361 ggcggcttca acgaaattgt gtccgtcgaa gttttcgcca cgcaaagcga caaataactc
    12421 gccgcctttg agcgttcgcg aatcggtcct gatcttgtcg atcacaatcg ttccccttcc
    12481 ggaggaaagg gaagccccgg caaatttcgc aatttgagaa attggaagcg gatccataac
    12541 taaaactcca tcggatggtc ctcgatcgcg cgccgcgcga cttgaatgtc gtcgaatggg
    12601 acggtgtgat cggcgaactc ctgataattt tcgtgcccct tgcccgcgat caagataatg
    12661 tcgcgcggct gtgccaggct gacggcgcgg ccaattgcct ctgttcgatc gatcattttc
    12721 tcatagcgat ttgagcgaaa gcctttttcg atctgatcaa tgatagcgcc cggatcctct
    12781 ttgcgcggat tatcggaagt gatgatcgcg taatcggcat ttcgatccac gatctcgccc
    12841 atcaacggac gcttttgccg gtcgcgattg ccgccgcagc cgaaaacgac aatcaagcga
    12901 ctcggttcca gttcgtgcag agtcttcaga acattctgaa gcgcatccgg cgtgtgcgca
    12961 taatcgacga aaacctgaaa ctgtcgcttg gccggaacga tttccaggcg ccccggtacc
    13021 tgcggagatt tacctagact gaggactgcg tcacgcagac taattcccaa agcgttggct
    13081 gccgccaacg ccgcgattga gttagccaca ttaaaccggc cgatgagcgg aacgcggacc
    13141 agataactct tgccccgggc atcgagctga taggaagtgc cactaaactc cacgcgataa
    13201 tttgaagcgc gaaaatccgc ccgcgcgctc atgccgcacg taatcaccgc gacgcgcttg
    13261 tcgattttcc caagcagttg ttcgccatag cgatcatcga tattcacaat tgcgacgggc
    13321 ttcagttttc tttcctgact cccgagttgg gcaaaaagtt tcgccttgga atcgaagtag
    13381 ctctccatcg ttccatgaaa atcgaggtgg tcctgtgtga ggttggtaaa aacggcgaca
    13441 tcccattcga ggccacgcgt gcggtcttgc gcaagcgcgt gcgacgaaac ttccatcgcc
    13501 gccgcccggc aaccggcgtt cgcgatttgc gccagcagtt cctgcaaatc aagcgattcg
    13561 ggggttgtgc gaatggcggg caaaacacgt tccccaattt cgtagcgcac cgttccaatc
    13621 aatccgcaac gcagacccgc tttttcgcag atatatttga taagaaatgt ggtggtggtt
    13681 ttcccgttcg tgccggtcac cgctgccaat tttaatttcc gcgcgggatg cccgtaaaaa
    13741 gttgcagaga gatcagcgag cgcggcgcgc gtattttcga cgacgaggca ggtgacgcgc
    13801 ggagtttttt cctcgcgttc cgtaacgatt actgatgcgc ctttttcgat tgcctggccg
    13861 atgaattcgt gtccatcggt tttttcaccc cgcagcgcca cgaacatgcc attcctttgc
    13921 attcgccgcg agtcatacgc gatgctctct acggggcgat caagcgtccc gatgacgtgg
    13981 cgaactggaa tcgcggcgac aagagttttg agctgcatgt ggtttcaaca tcgccgccgc
    14041 gagacggatt cgcggcggcg agccggggca aggcgttcaa gggcgcgagg cattggttaa
    14101 ggcgactcgg tcggctggca cagtctttcg gcctcgctcc gccggcacgg ctttttgaac
    14161 gcgctccaca gggatggctt tgcgaatctg ctcttgcggc cgaagatcga gatattgggc
    14221 agccttttcg gctacccggg aaaagatcgg tccggcaata agcccgccgt aattcaactc
    14281 tggtttgctg gtctgcgcgt cgtccagcac taccagaccg acaaactcgg gatgctccgc
    14341 tggcaaatag cctgcaaagg aaacgacgta cttaccatgc tcatatcccc ccctgggatc
    14401 gaccttctgc gccgtcccgg tcttgccggc aatcgtaaaa ccaggaacag ccgccgcagc
    14461 ggcagtgccg cgatcgctta cgacacctcg aagcgcgtcg ccaatttgcc gggccgtttc
    14521 cggcgaaatt acctggcgcg ccgtcaccgg ggaaagtgag ctgatggatt tgccgtccga
    14581 agtagtaatc gatttcacaa tgcgtggagt cattaatttc cccccattgg caatcgtcgc
    14641 cattgccgcc gtcatttgta atggggtgac cccgatttcg tgccccatcg gaatccgcgt
    14701 aatcgaaatt ttgctccact tttgcggagg acgaatgagc ccgttaattt caccgggaag
    14761 ttcaattccc gtgcgctcgc caaatccaaa gcggcggacg tattcgtaaa acttttgttc
    14821 acccacgctg atcgcgagct tggcggcgcc gatgttgctc gatctgatga gaatatcgcg
    14881 cacgctcaga tacgaaaacg cccgatgatc gtgcagggct gtgccgttga aattccagag
    14941 gccattttca caaaagacgg tcgaatcaga ccgaagcttg cgttcgttga gcgccgcggc
    15001 cgccgccacg atcttgaacg tggaacctgg ctccatcatg tcgatgatgg cgcggttttt
    15061 catctcttcg ggcttcgcct cggagcgaag gttcaaatcg aaattcggcc gattcgccat
    15121 cgcaaggatt tcacctgtct gcggccgcat caaaatgatc gtggcttttt ggggggaata
    15181 ctccgccatc gcagcgtcga tctcgttctc aactatgttc tgcaagctga gatcgacagt
    15241 aagatggatc tggcagccgt tgcgcggcgg gtgttcctgg cctcggtacg gcacgatctc
    15301 ctggccggca cgattgtgct cgatgaagcg atagccgtcc tgtccatgaa gatattcctc
    15361 catcgacgct tccacgcctt gaatgccatg atggtcgaaa tcggtaaatc cgattacgtg
    15421 gcagagcatg gagccatttg gatagatgcg gctggagtcg tgttcgaaat aaatcccccg
    15481 caaatttttc gcgcgaagct tttcgcgcag tgaacggctg gccgtttccg gcacttcacg
    15541 cttgatcaca atgtatcggc gctcgctgct taacttttcc gcaacctgct cgaccgggat
    15601 gctcagttcc ctgctgacaa gatccacaac tgcttcccga tcgttgaaaa gggtggcatc
    15661 agccaccacc gtttcgacag ggatgttgtg cgcgaggact tcgctgttcg cgtccaggat
    15721 cgagcctcgc tccgcgtaaa tgatttgttt gtaaacgtgc ttttcggctg cgagagccgc
    15781 atactcgtcg tgcttgataa cttgcagata aatcaggcga aacgaaaaga ggc
//
LOCUS       DASQUQ010000029         9490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_909250, whole genome shotgun sequence.
ACCESSION   DASQUQ010000029 DASQUQ010000000
VERSION     DASQUQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909250"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VE486_00805"
     CDS             <1..456
                     /locus_tag="VE486_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZA37657.1"
                     /translation="LGDRATMINSQDDRVFMRSMATRGQAGGLSPATHDCLALLAQSN
                     FDYVIIETVGTGQEAMPFQKNGIVDLTVLVMNPDYGSRLQLQKIVMLDLAEIVVVNKS
                     DLQRARTAHTEIEQRLDQNRRAQRLIDTVAKRHRDPGVDKLFELITQRG"
     gene            616..2439
                     /locus_tag="VE486_00810"
     CDS             616..2439
                     /locus_tag="VE486_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZA37658.1"
                     /translation="MSKLGQVVESVEKYNKFVLDQVKRARTDEKFGRELVNRWNEIKA
                     KTPLTHTPTGLPLPRLALPEIDEPGEIARYLFGEGIPGEFPFLNGAYREMYLEPVREI
                     ETGSYGKKTSTNGATKKATGNGAKPPEEPTRLFSGLMLAEDTNERFHYLTQHQRTHRL
                     STAFDGPTLYGIDSDADGVFGKIGEGGVAIDTIEDMVRLYDGFDLGSPNFSASMTISG
                     PAPIIMAMYIAAAKRRFGPKVVPKLRGTIQADIFKEVQAQNETIFPIEASLRFLTDMV
                     EFTTREMPRWYPISISGYHIGEAGSTPVQQAAYTLSNGFAYAEMFAARGIPVDQFGPR
                     LSFFLDCGLDAEYIALARVSRRIWAIGMRDVFGAGPRGQLYKLHTQTSGRSLIAAEFK
                     NNLTRTAAELMLAYMNATNSCHSNSADEPFTTPSEEWIRLAAHGQAILLEESGIFKHT
                     MNMLSGSPGMKAVERAVEAAILDEFREIERLGGVLAAVEDRFQRSQIQNAAHRYEQQI
                     YDGTRPIIGLNKYRNGEDDAPKIKLARTPRVKQQLQVDRLAKFKKKNAEKAKRALDKL
                     VDVVERGENCFPTLLDAVEVSSLGQITGRLQEVVGRFRPMV"
     gene            complement(2507..2929)
                     /locus_tag="VE486_00815"
     CDS             complement(2507..2929)
                     /locus_tag="VE486_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37659.1"
                     /translation="MNRKSVALCFSIFLLMAIGLVAAEAPNFAGEYADRKFLNGQAVF
                     QMSLEPSGDTVSVWFGAANNDGSGAAPEAEGTGKVSGKGVLAFKWKDSFKNSGTGRVT
                     RAGDDIIVSMKTARVSDPRCLAFYGQKMRLKPVKKKEE"
     gene            complement(2963..4993)
                     /locus_tag="VE486_00820"
     CDS             complement(2963..4993)
                     /locus_tag="VE486_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZA37660.1"
                     /translation="MRSPIGLLVPLARRKLIGVSEFQFGGQFVWHPTPELVAQSNLQR
                     FIEKHALGSYRELMRRSTTDIAWFWNTVLHDLDIQFYKPYSQVVDLSEGKPWARWCVD
                     GEMNIVHNLLDKYAGTPIDNHLAIKSEIEDGSTRTLTYKELREEVNKMAATLRGLGLG
                     KGDAVGVFMPMVPEIVVAMLAIIKIGGIFLPLFSGFGASAIVSRLKDAEAKALFTAGG
                     TYRRGKFCPMKPIADEAASQIPTLRHLLVLNQAGEWLLEDLSPDARPPTKLSGPFDDA
                     STKRTSAEDPMMIIYTSGTTGKPKGAVHTHCGFPIKSVQDMWHGLDLHSDETLFWMTD
                     MGWMMGPWEVFGTLLLGATMMLYDGAPDFPGPDRVWSLVDRHKVTALGVSPTLIRALR
                     SRGGGSDEIVHRHDLSSLRKFASTGEPWNPDPWMWLFQNVGGGKLPIINYSGGTEISG
                     GIVMGNILTPMKPCAFSGPLPGMAADVVDENGKSVRGKVGELVIREPWIGMTRGFWKD
                     RERYIESYWSRLPDVWVHGDWAAVDDDGLWYILGRSDDTIKIAGKRLGPAEVESILVA
                     HPQVSEAAAVGVPDPIKGEALICFCVLRKGINGDVDLAAELKGIVARDLGKALAPHDV
                     LFVGDIPKTRNAKVMRRLLRAAYIGEKLGDTSALENPASLEEIQRVATRKVD"
     gene            5183..6604
                     /locus_tag="VE486_00825"
     CDS             5183..6604
                     /locus_tag="VE486_00825"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZA37661.1"
                     /translation="MATTLAHQLADYACALNFEDLSPEVVREVKRRVIDSLGCALGAW
                     DEKPCAIARKVASEFSAKHGSTIFGTNHKAPPDWAAFANGCAIRYFDYNDTYLSKEPA
                     HPSDNISAALAIAESVGANGRELITAIALAYEVQCRLSDAASIRARGWDHVTYGAFST
                     ALASAKLMNLDPERTRHAVNIAGVAGAAMRQARVGELSHWKGVAFATAARHGVYSALL
                     ARAGMTGPGPIFEGQMGFEKQLGVSLGNVGEKFAVPFAKTNHGPASMILKTSIKNWPA
                     EYHSQSAIEAALFLREEVIDPSTPLGTNLARVKSVTIESHDASVDIIGSEAEKWRPAT
                     RETADHSLPYITAIALIDGEVSNKQFEPERFTDPKVWKFLKKVNVERNAELSAMYPGA
                     VANIVHVDLSDGQRLTKRVDYPLGHARNPLQDSEVEGKFFALVTPALGEKAAREIVDL
                     VWKLGDAKNVDELMEATRMPNDK"
     gene            6730..7590
                     /gene="prpB"
                     /locus_tag="VE486_00830"
     CDS             6730..7590
                     /gene="prpB"
                     /locus_tag="VE486_00830"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR02317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HZA37662.1"
                     /translation="MSTGKSRRLRDLIAKGCVALPGVPNAAMARQVERAGFDAVYISG
                     AGMANATAGVPDIGLLTSTEVAQLAGYIANAVKIPAIVDADTGFGGAENVARTIRELE
                     QVGLAGCHIEDQEFPKRCGHLAGKEVVAVDEMVGKIKAAARARRDHDFFIIARTDARA
                     VEDFDSAVERAQRYVEAGADGIFPEALQSSEEFKAFARKVKAPLLANMTEFGKSPLLS
                     FQELSDFGYRMVIFPMSAFRVSMKASDEFLRDLKKRGTQSGWLDKMQTRKELYDLLDY
                     DPTAESWHDR"
     gene            7651..8787
                     /locus_tag="VE486_00835"
     CDS             7651..8787
                     /locus_tag="VE486_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZA37663.1"
                     /translation="MSNETKPEYSPGLAGVIAGETAICWVDPNAGLMYRGYDIHEMAQ
                     KASFEEVAYLLLNGELPNGKQLVEFTQQIAAQRALPGPVIEMLRLLPSTTHPMDMLRT
                     GVSMLSAFDKDLSDNSHDANIRKSIRLIARVSTLITDGWRISHGQQPLPERPDLTQAG
                     NFFYKLEGNVPQDWKIRMLDTIFNLYADHEFNASTFAARVTASTLAGIYAAVTSAVGT
                     LKGPLHGGANEESMKMLEEIGTPDRAEAWLIKKLETKEKIMGFGHRVYKKGDSRVPVM
                     REIGRDLGKRTGKEQWIPICEKLEEVMEREKHLCANVDLYAAPVFWMLGFPPELDTPL
                     FAAARVAGWCAHVTEQHDNNRLIRPRSLYIGPKLRPYPGRSGGA"
     gene            8838..9302
                     /locus_tag="VE486_00840"
     CDS             8838..9302
                     /locus_tag="VE486_00840"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZA37664.1"
                     /translation="MSAQSLQEKYAPDNACWGCGPANAEGLHIRSFPKDGEVVAEWKP
                     EKKYEAFPGVLNGGIIGTLLDCHCNWTAAYHLMKRAKTDRPPCTVTAEYSIKLLRPAP
                     TSDSVFLSAKVVDLTDDRATVEGTLSAGGKVCATCRGIFVAVKEGHPAYHRW"
BASE COUNT         2180 a         2576 c         2731 g         2003 t
ORIGIN      
        1 ctgggcgatc gcgccaccat gatcaactcg caagacgatc gtgtctttat gcgcagcatg
       61 gccacacgtg ggcaagcggg cggactttca cctgcgacgc atgattgtct cgcgctactc
      121 gcgcaatcga acttcgacta cgtcattatc gagacggtcg gcaccggcca ggaagcgatg
      181 ccgtttcaaa aaaatggaat tgtcgatctt acggtgttgg tgatgaatcc cgattacggc
      241 agccggttgc aactgcaaaa gatcgtcatg ctcgatctcg ccgagattgt tgtcgtgaac
      301 aagagcgatc ttcaacgcgc gcgcacagcg cacacggaga tcgagcaacg gctcgatcaa
      361 aatcgacgcg cccagcggct aatcgacacg gtcgcaaaac ggcaccgtga tcctggcgtg
      421 gataagttgt ttgagctaat cacccagcgt ggctgagcat cgagtggtca ccatcttttt
      481 gtcatgttga gcgaagtcga aacatctcta gctttgctct gaaaatatta caggttcttc
      541 gacttcgctg cgcttcgctc agaatgacag aggaaggaag acgtggcgag gaatgaaaag
      601 cgcggaatta ttaccatgag caagcttggg caagtcgttg agtcagttga gaaatataac
      661 aaattcgtgc tcgatcaggt gaagcgcgcg cgcacggatg aaaaattcgg acgcgagctt
      721 gttaatcgct ggaacgaaat caaagcgaaa acgccgttaa cgcacacacc caccggcttg
      781 ccattgccgc gcctggcatt gccggagatc gatgagccgg gcgaaatcgc gcgttatctt
      841 tttggcgaag gaatcccggg tgagtttccg tttctgaacg gcgcatatcg cgagatgtat
      901 ttggagccgg tgcgcgagat cgaaacgggc agctacggca agaaaacttc caccaacggc
      961 gcgacgaaaa aagccactgg aaatggcgcc aaaccacccg aggaaccgac gcgcctgttc
     1021 tccggtttaa tgctggccga ggacacaaac gagcgtttcc attacctcac gcagcaccag
     1081 cgcacccatc ggcttagcac cgcctttgat ggaccaacac tttacgggat cgacagcgac
     1141 gcggacggcg tgtttggaaa aatcggcgaa gggggcgtgg ccatcgatac gattgaagac
     1201 atggtgcgac tttacgacgg gttcgatctc ggctcgccaa atttctccgc gtcgatgacg
     1261 atcagcggcc cggcgccgat catcatggcg atgtatatcg cggcggcgaa acgccgtttc
     1321 ggtccgaaag ttgttccgaa gctgcgcggc acaattcagg ccgacatttt caaggaagtc
     1381 caggcgcaga acgaaactat ttttccgatc gaagcatcgc tgcggtttct cactgacatg
     1441 gtcgaattca ccacgcgcga aatgccgcgc tggtatccaa tctcgattag cggttatcac
     1501 atcggcgaag ccggttcaac gccggtacag caggcggcct acacactctc aaacggcttc
     1561 gcctacgccg aaatgttcgc cgcgcgcggc attccggtcg atcaattcgg gccgcgcctc
     1621 tccttctttc tcgattgtgg actggatgcc gaatacatcg ccctggcgcg cgtgtctcgc
     1681 cgaatttggg cgatcggcat gcgcgatgtg ttcggcgcag gaccgcgcgg tcagctttac
     1741 aaattgcata cccaaaccag cgggcgttcg ctcatcgcgg cggaattcaa gaataatctg
     1801 acgcgcaccg cggcggagtt gatgctcgct tatatgaacg cgacgaactc atgtcactca
     1861 aacagcgctg acgaaccgtt cacgacgccg agcgaagaat ggatccggct tgccgcgcac
     1921 ggacaggcga tcttgctcga ggaatcgggc atttttaagc acaccatgaa catgttgagc
     1981 ggctcgccgg gtatgaaagc agtagagcgc gcggtggaag cggcgattct cgatgaattt
     2041 cgcgaaatcg agcggctcgg cggagtgctg gcggcagtcg aagaccgttt ccaacgcagt
     2101 caaatccaga atgccgccca tcgttacgag caacaaattt acgatgggac tcgtccgatc
     2161 atcggcctga ataaatatcg caacggcgaa gatgacgcac cgaaaataaa actcgcccgc
     2221 acgccgcgcg tgaaacagca gctccaagtt gataggcttg cgaagtttaa aaagaagaac
     2281 gcggagaaag cgaagcgcgc gctggataaa cttgtcgatg tagtggagcg cggtgaaaat
     2341 tgttttccga ctttgctcga cgcggtcgaa gtctcctcgt taggccaaat cacgggtcgg
     2401 ttgcaggaag tggttggccg attccgcccg atggtgtaac actggcatcc tgcctgttta
     2461 gtcgagcagg cttccagcct gcagtcgcgg agcacgcgag acgcctctac tcctccttct
     2521 ttttgacggg tttgagccgc attttctgtc cgtaaaacgc caggcagcgc ggatccgaga
     2581 ctcgcgctgt tttcatcgat acaataatgt catcgcccgc gcgcgtgacc ctgcccgtgc
     2641 cggaattctt gaaactgtct ttccacttga atgccagcac gcctttgcca ctcacttttc
     2701 ccgtcccctc cgcttctggt gccgcgccgg agccgtcatt gttcgcggcg ccgaaccaga
     2761 ccgacacggt gtctccgctt ggctctaggc tcatttgaaa tacggcctga ccgttcagga
     2821 atttcctgtc cgcgtactca ccggcgaaat ttggtgcttc cgcagcgacg agtccgattg
     2881 ccatgagaag gaagatcgaa aaacagagag caacagattt gcgattcata cagcacaacg
     2941 taacaaacgc ggccgatcca ggtcaatcga ccttccttgt ggcaacccgc tggatctctt
     3001 ccagactcgc aggattttcc agcgcagaag tgtcgccgag tttttcgccg atgtaagcgg
     3061 cccgaaggag gcggcgcatc acttttgcgt tccgcgtttt tggaatgtca cccacgaaaa
     3121 gcacatcgtg gggcgcgagc gccttcccga gatcacgcgc gacaattccc ttcaattcag
     3181 cggcaagatc gacatctccg tttatgcctt ttctcagaac gcaaaaacaa atgagcgctt
     3241 cccctttgat cggatcaggc acgccaaccg cagccgcttc gctgacctgc ggatgcgcga
     3301 cgagaattga ttccacttcg gccggtccga gacgtttgcc cgcaatctta attgtgtcgt
     3361 cggagcgccc taggatgtac caaagaccgt cgtcatcgac tgcggcccaa tcgccgtgta
     3421 cccacacatc aggcaaacgc gaccagtagc tttcgatgta gcgctcgcga tctttccaaa
     3481 acccgcgtgt cattccaatc cacggttcgc gaatgaccaa ttcgccaact ttcccgcgga
     3541 ctgattttcc attttcatca acaacatctg ctgccattcc gggcagcggc ccggaaaagg
     3601 cgcacggttt catcggcgtg agaatgttcc ccatgacgat gccgcccgaa atttccgtgc
     3661 cgccggaata gttgatgatc ggaagttttc cacctccgac gttttgaaat agccacatcc
     3721 acggatcggg attccacggt tcaccagtgg acgcgaactt gcggagcgat gaaagatcgt
     3781 gtcgatgcac gatctcgtcg ctcccgccgc cgcgggagcg cagcgcgcga atcaaggtcg
     3841 gcgagacacc cagcgctgtc actttatggc gatctaccaa tgaccagaca cgatcgggtc
     3901 ccgggaaatc gggcgcgcca tcgtaaagca tcatcgttgc gccaaggagc agcgttccga
     3961 aaacttccca cggtcccatc atccagccca tgtcggtcat ccagaaaagc gtttcgtctg
     4021 agtgaagatc gagaccatgc cacatatcct ggacagactt aatcgggaat ccgcaatggg
     4081 tatgcacggc tccttttggt tttccggtcg tgcccgacgt gtaaatgatc atcattggat
     4141 cttcagctga tgtgcgcttg gtggacgcat catcaaaggg ccctgacagc ttcgtcggcg
     4201 gccgtgcatc ggggctgaga tcttcaagga gccattcgcc ggcttggttg agaacaagga
     4261 gatgccgcag cgttggaatc tgcgatgccg cttcatcggc gatcggtttc atcgggcaaa
     4321 atttcccgcg acgataagtt ccacctgccg tgaagagtgc cttcgcctcg gcatctttca
     4381 gccgggaaac gattgcggac gcgccgaagc cggagaacag cggcagaaaa atcccgccga
     4441 ttttgatgat cgcgagcatg gcaacgacga tctcgggaac catgggcatg aacacgccga
     4501 cggcgtcgcc tttgccgagt cctaggcccc gcagcgtggc ggccatctta tttacctcct
     4561 cgcgcagttc tttgtaagtc agcgtgcgcg ttgagccgtc ttcgatctcg gatttgatgg
     4621 cgagatgatt gtcgatcgga gtgccggcgt atttatcaag caggttatgg acgatgttca
     4681 tctcgccatc gacgcaccaa cgcgcccatg gcttgccctc gcttagatcg acaacctgcg
     4741 agtacggttt gtaaaactga atatcgagat cgtgcagcac agtgttccag aaccaggcaa
     4801 tgtcagtcgt cgatcgtcgc atcaattccc tataagaccc gagcgcgtgt ttctcgatga
     4861 accgttgcaa attggattgc gcgaccagct ccggcgtcgg atgccacaca aattgtcctc
     4921 caaattgaaa ttcgctcacg ccgataagtt tgcgccgagc gagcggcacg agcaagccga
     4981 ttggggagcg cacgcggccc gcgtgttcat ttcggcggcc tcgccgaaat gtgcagatgc
     5041 gacgttcggc agttgcctag cgccccgcga ttttggcgag ccgccaaaat gtgcacgcca
     5101 gcggcgtgcg ctccccagaa ttccccgatc tcgcgtcttg ctcgcttcca attcatcggc
     5161 agattgcagt ttccattttc caatggccac tacgcttgct catcaactgg ctgactacgc
     5221 gtgcgcgctt aactttgagg atttgtcgcc tgaagtagtg cgcgaagtga agcggcgcgt
     5281 gatcgattca ctgggttgcg cgctcggggc ctgggatgag aagccgtgcg ctatcgccag
     5341 aaaagttgct tcggaatttt cggcgaaaca cggatcgaca atcttcggca caaatcacaa
     5401 agcgccgccg gattgggcgg ccttcgcgaa cggctgcgct atccgctatt tcgattacaa
     5461 cgacacgtat ctgtcgaagg aaccggcgca cccgagcgat aatatttccg ccgccctggc
     5521 gatcgcggaa agcgtcggag cgaatgggcg cgaattgatc acggcaatcg cgctggctta
     5581 tgaagtccaa tgccggttgt ctgatgctgc aagcatccga gcgcgcggat gggaccatgt
     5641 gacatatggc gcgttttcga ctgcgctggc cagcgcgaag ctgatgaatc tcgatccgga
     5701 aagaacgcgg cacgcggtga acatcgcagg cgtagcaggt gcggcaatgc gtcaggcacg
     5761 cgtaggcgag ctctcgcatt ggaaaggtgt cgcgtttgcc actgccgcgc ggcacggcgt
     5821 gtactccgct ctgcttgcac gtgccggaat gaccggacca ggaccgattt tcgaaggcca
     5881 aatgggtttc gagaaacaac tcggcgtctc gttgggcaac gttggagaaa aattcgccgt
     5941 tccatttgcg aaaaccaatc acggtccagc ctcgatgatc ttgaaaacaa gcatcaaaaa
     6001 ttggccggcg gaatatcaca gccagagcgc gatcgaagcg gcattgtttt tgcgcgaaga
     6061 ggtgatcgat ccttcgactc cgctcgggac aaaccttgcc cgggtgaaat cggtaacgat
     6121 tgagagtcac gacgcctcgg tcgatatcat tgggagtgag gcggaaaaat ggcgtccagc
     6181 aacgcgcgaa acggcggacc acagtttgcc ttacatcacg gcgattgcat tgattgacgg
     6241 cgaagtctcg aacaagcagt tcgagccgga acgctttaca gatccaaagg tttggaagtt
     6301 tttaaagaaa gtgaacgtgg agcgtaacgc cgagttgagc gcgatgtatc cgggcgcagt
     6361 cgcaaacatc gtgcatgtcg atctcagcga cggacaacgg ctaacgaagc gcgttgatta
     6421 tccgcttgga cacgcaagaa acccactgca ggactccgag gttgaaggga agttcttcgc
     6481 gctggtaacg ccggcccttg gtgaaaaggc tgcgcgtgaa attgtcgatc ttgtgtggaa
     6541 attgggcgat gcgaaaaacg tagatgaact aatggaggcg acgagaatgc cgaatgacaa
     6601 atgacaaatg tcgaaaaatg acgaagccca aatgacgaat tggtctcgac gcgttgtggc
     6661 gtgttttcgt tattcgtcat ttggatttcg ttcgtcattc gtcattcgtg cttcgtcatt
     6721 tttcggaaca tgagcaccgg caagagcaga aggctgcgcg atttgattgc caaaggctgt
     6781 gtggctctgc cgggcgttcc gaacgcagcg atggcgcggc aggttgagcg cgcgggtttt
     6841 gatgcggttt acatcagtgg ggcgggaatg gcgaatgcaa ccgcaggcgt gccggacatc
     6901 gggctgctta cttctacgga agtcgcgcag ctcgcgggtt atatcgccaa cgcggtgaag
     6961 attccggcaa ttgtcgatgc tgacacggga tttggcggag cggaaaatgt tgcgcgcacc
     7021 attcgcgaac ttgagcaggt cgggctggcc ggctgtcaca tcgaggatca ggaatttccc
     7081 aagcgatgtg gacatctagc cgggaaagag gtcgtcgctg tagacgaaat ggtggggaaa
     7141 atcaaagcgg cggcaagggc gcggcgtgac cacgattttt ttatcatcgc gcgcacagac
     7201 gcgcgggccg ttgaggattt cgacagtgca gtggagcgcg cgcagagata tgtcgaagcg
     7261 ggcgcggacg ggatttttcc ggaagcgttg caaagcagcg aggaattcaa agcgttcgcc
     7321 cggaaagtta aagcgccgct actcgcgaat atgacggagt ttggcaagag tcccctgctc
     7381 tcatttcaag agctgtccga tttcggatac agaatggtga ttttcccgat gagcgcattt
     7441 cgcgtttcga tgaaagcgag cgacgaattc ttgcgcgatc tgaaaaagcg cgggacacaa
     7501 tcgggttggc tcgacaaaat gcagacgcgt aaggagcttt atgatctgct cgattacgac
     7561 ccgactgccg agagttggca cgaccgctaa atcgttaaat cgctgaatcg tttgaacgtt
     7621 cccgattcgc gagtaagaaa gaacagaccc atgagcaacg aaaccaaacc agagtacagt
     7681 cccggcctgg ccggggtgat tgcgggcgaa acagcgatct gttgggtcga tccgaatgcc
     7741 gggctgatgt atcgcggcta cgacattcat gaaatggcgc agaaggcgag ttttgaggaa
     7801 gtcgcttacc tgctgctgaa cggcgagttg cccaatggga aacaactggt cgagttcact
     7861 caacaaattg ccgcgcaacg cgcgctgccg ggcccggtga tcgaaatgct gcggctgctg
     7921 ccgagcacga ctcatccgat ggatatgcta cgcaccggcg tctcgatgtt aagtgctttc
     7981 gataaagacc tgagcgacaa ctcgcatgac gctaatatcc gcaagtcgat tcgcctgatc
     8041 gctcgcgttt ctactctaat caccgatggc tggcgcatct cgcacggtca acaaccgctg
     8101 ccggaaaggc ccgatctcac gcaggccggc aatttctttt acaaattgga gggcaacgtt
     8161 ccgcaggatt ggaaaatccg gatgctggat acaattttca acttgtacgc cgaccacgaa
     8221 ttcaatgcct ccacgtttgc tgcgcgggtg accgcctcca cgctggccgg catctacgca
     8281 gcagttacct ccgcagttgg gacgctcaag gggccgttgc atggcggcgc caatgaagaa
     8341 tcgatgaaga tgctggagga aatcgggacc cctgatcgcg ccgaagcctg gctgataaaa
     8401 aaactggaga ccaaagaaaa gatcatgggt ttcggccatc gcgtttacaa aaaaggcgat
     8461 tcccgggtgc cggtgatgcg tgagatcggg cgcgatctgg gcaagcgcac cggcaaggaa
     8521 caatggattc cgatttgcga gaagctcgag gaagtgatgg agcgcgagaa acacctctgc
     8581 gccaatgtgg atctttacgc ggcgccggta ttttggatgc tcggattccc gccggagctg
     8641 gatacacctc tgttcgccgc agcgcgcgtg gcgggctggt gcgcgcacgt gactgagcaa
     8701 cacgataata atcgtttaat tcggccacgc tcactttaca tcggacctaa attgcgcccc
     8761 tatcctggcc ggtccggggg cgcataagat cgacatggca gggcgcgatc gctgagcccg
     8821 ccgggccagc aaccccaatg agcgcgcagt ctttacagga aaaatacgca ccagacaatg
     8881 cctgctgggg ttgcggcccg gcaaatgctg agggattgca tattcgaagt ttcccaaaag
     8941 acggcgaggt agtagcagaa tggaaaccgg agaaaaagta tgaggctttt cccggagtgt
     9001 tgaatggcgg cattatcggg acactgctcg attgccactg caactggacg gcggcgtatc
     9061 atttgatgaa gcgcgcgaaa acggaccgcc cgccgtgcac ggtcactgcc gagtactcga
     9121 ttaaattgtt gcgaccagct ccgacgagcg attcggtctt tctttccgcg aaagttgtcg
     9181 atctaaccga tgatcgcgcg accgttgaag gaacattgag cgccggcgga aaggtttgcg
     9241 ccacttgtcg cggaattttc gtcgcggtga aagaagggca tccggcgtat catcgttggt
     9301 aaaatcctgg cggtcaggca tagcctaatt tattcttatg ccaccgtaaa aaaccgaaac
     9361 tttagctcgc ggccggggtg taatgtgtgg aaaccaaatc tatttatgaa ttcaaaacgt
     9421 atattttgcg taatctgctg tgctgcggtt ctgatgaccg cgccggttgc gctgagtggc
     9481 tcgcgcggtg
//
LOCUS       DASQUQ010000030         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_998291, whole genome shotgun sequence.
ACCESSION   DASQUQ010000030 DASQUQ010000000
VERSION     DASQUQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998291"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VE486_00845"
     CDS             <1..343
                     /locus_tag="VE486_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HZA37665.1"
                     /translation="SAAYMGRWIAKNVVASGLADRCEVQFAYAIGHPEPVSVSVDTFC
                     TGKVDEEKLERAIWEVFNFKPAEIIKQLNLLRPIYRKTTNYGHFGRIDDLDSLTWERT
                     DKAEALQKAVK"
     gene            378..806
                     /locus_tag="VE486_00850"
     CDS             378..806
                     /locus_tag="VE486_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HZA37666.1"
                     /translation="MQKDPQSHAIIGAAMEVHRKLGFGFLEAVYQCALALEFQQRTIP
                     FKAEVALPIRYKGKLLTCAYRADFICFEDLLVETKAIAELTRVDDAQVINELKATDLE
                     RGLLLNFGAPSLQFKRLVLTPSENLCQSAKSADDFQEIVL"
     gene            803..2233
                     /gene="ahcY"
                     /locus_tag="VE486_00855"
     CDS             803..2233
                     /gene="ahcY"
                     /locus_tag="VE486_00855"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZA37667.1"
                     /translation="MKTSTIEQTKTDYKIADISLADFGSKEISIAEKEMPGLMAIRET
                     YASQKPLAGVRITGSLHMTIQTAVLIETLVDLGASVRWASCNIFSTQDHAAAAVAKSG
                     VSVFAWKGESLQDYWWCTYRAISYPDGKGPQLVVDDGGDATLLIHKGYELEEGSDWAK
                     SKSANKEEQVIKDLLLEIQRENPYRWHEIVKDWRGVSEETTTGVHRLYKMHQEGRLLV
                     PAINVNDSVTKSKFDNLYGCRHSLVDGLNRALDVMLAGKIAVVCGYGDVGKGCAQSLR
                     GQGARVVITEIDPINALQAAMEGYEVTTIENTLGRGDIYVTASGNIDVLTLRHMEQMK
                     DQAIVANIGHFDNEIQVDALNTAKGVKKTNIKPQVDKYTFSDGHEIFLLAEGRLVNLG
                     CATGHPSFVMSNSFSNQVLAQLDLWKNRDAYKVGVYVLSKKLDEEVARLHLEKIGVKL
                     TKLTKEQADYLGVPVEGPFKSEHYRY"
     gene            complement(2257..2607)
                     /locus_tag="VE486_00860"
     CDS             complement(2257..2607)
                     /locus_tag="VE486_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37668.1"
                     /translation="MRTLSIFGIAVVLASCAQPEKLVAPSSAAAAGSPFDQVGKVTLY
                     PGQPCTSQIMFLFRADRPVSTISLAAPMPETRILTEAANRRGRVHVRGKWRRTKQTGC
                     SYVEVKEAEIKSSF"
     gene            complement(2844..>3107)
                     /locus_tag="VE486_00865"
     CDS             complement(2844..>3107)
                     /locus_tag="VE486_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HZA37669.1"
                     /translation="LYSNHSCDPNIGMRGEITFVAMRDIRGGEELTHDWATTDDDDYS
                     IECKCGAPDCRGTLTGKDWQCAELQKRYAGYFSAYLAKKIAQR"
BASE COUNT          749 a          818 c          833 g          707 t
ORIGIN      
        1 cagtgccgct tacatggggc gttggatcgc aaagaatgtc gttgcatccg gcctggctga
       61 tcgatgcgaa gttcaattcg cttacgcgat cggccatccc gagccagtga gcgtcagcgt
      121 cgatactttc tgcaccggta aagtggatga agagaaactt gagcgtgcca tctgggaagt
      181 cttcaatttc aaacctgccg agattatcaa gcagctcaat ctgcttaggc cgatctatcg
      241 caaaacgacc aactacggcc acttcggccg catcgacgat cttgattccc tcacttggga
      301 aagaaccgat aaggccgaag cgctccagaa agcagttaag taatccacag atttcgcaga
      361 tttacgcaga ttacaaaatg cagaaggatc cacagagtca tgcgatcatc ggagctgcga
      421 tggaagtgca tcgaaagctc ggattcggat ttctcgaagc ggtctatcaa tgcgctttgg
      481 cgctcgaatt tcagcagcga accattcctt tcaaggctga ggttgccttg ccgattcgct
      541 ataagggcaa actcctgacc tgtgcgtatc gcgcagattt tatttgcttc gaagatttgc
      601 tcgtcgaaac aaaggcaatt gctgaactaa cacgagttga tgacgcacaa gtgataaacg
      661 agctcaaggc cacagatctt gagcgcggcc tgttgctaaa tttcggggcg ccgagtctcc
      721 agttcaagcg acttgttctg acgccttctg aaaatctgtg tcaatctgcg aaatctgcgg
      781 atgactttca ggaaatcgtt ttatgaaaac atcaacaatc gaacaaacca aaacggatta
      841 caaaatcgcc gatatttcgc tggccgattt tggaagcaag gaaatctcga tcgcggagaa
      901 ggaaatgccg ggtctcatgg cgatccgcga aacatacgcg tcgcagaaac cgcttgcggg
      961 cgtccgcatt accgggtcgt tgcacatgac gatccagacg gcggttttga tcgaaacact
     1021 tgtcgatctt ggcgcgagtg tgcgctgggc cagctgtaac attttttcga cgcaagatca
     1081 cgcggccgcg gcggtggcca agtcaggcgt ctctgttttc gcgtggaagg gcgaaagtct
     1141 tcaggattat tggtggtgta cttatcgggc gatttcgtat cccgacggca agggtccgca
     1201 actcgtggtt gatgacggtg gcgacgcgac gttgctcatt cacaaaggct acgaactcga
     1261 ggagggcagc gattgggcca aatcaaaatc cgccaacaag gaagagcagg tcatcaaaga
     1321 tttgcttctc gaaatccagc gcgagaatcc ctatcgctgg cacgaaatcg tcaaagattg
     1381 gcgtggggtt tctgaagaga ccacaaccgg tgttcatcgg ctctacaaaa tgcaccagga
     1441 agggcgactg cttgttcctg cgatcaatgt gaacgattcc gtgaccaaat cgaaattcga
     1501 caatctctac ggctgccggc attccctggt cgatggcctg aatcgcgcgc ttgacgtgat
     1561 gctggctggc aaaatcgcgg tcgtgtgcgg ttacggggat gtcggcaagg gctgcgcgca
     1621 atcgttgcgc gggcagggcg cgcgtgtcgt cattaccgag atcgacccaa tcaacgcatt
     1681 acaggccgcg atggaaggct acgaagttac caccatcgaa aatacgctgg gccggggcga
     1741 catttacgtt accgccagcg gcaacatcga tgttctcacg ctccgtcaca tggaacagat
     1801 gaaagaccag gcaatcgtcg cgaacattgg ccacttcgac aacgagatcc aggttgatgc
     1861 gctcaacaca gccaagggcg tgaagaaaac caacatcaaa ccgcaggtgg acaagtacac
     1921 gttttcagac ggacacgaaa ttttcctgct cgccgagggc cggctagtga acctcggatg
     1981 cgccaccggt catccgagct ttgtcatgtc gaactcattc tccaaccagg tgctcgctca
     2041 gctcgatctc tggaaaaatc gggacgccta caaagtcggt gtttacgtct tgtcgaagaa
     2101 actcgacgaa gaagtcgctc gcctgcattt ggaaaagatc ggtgtcaaac tcaccaagct
     2161 cacgaaagaa caagccgatt acctcggtgt cccggtcgag gggccattta agtcagagca
     2221 ttatcgttat tgagggacgc gggcagcggc gttctactaa aacgagcttt ttatttccgc
     2281 ttccttcact tcaacgtagc tgcacccggt ttgtttggtc cgtctccatt ttccgcggac
     2341 atggacgcgg cctcgacggt ttgctgcctc agtaagaatt ctggtttctg gcatcggcgc
     2401 agccagcgag attgttgaga ccggtcggtc cgcacgaaaa aggaacatga tttgtgacgt
     2461 gcacggttgt ccgggataca acgtgacttt ccccacttga tcgaagggag aaccggcagc
     2521 ggcagcgctg gacggtgcca ccagcttctc cggttgagcg cagcttgcta gcacaaccgc
     2581 aattccgaag atggaaagcg tacgcaaggg cccactcata ttcggctatt tttcttacac
     2641 aaactcttca ccgttccacg atctttccac ttttttgttt gcggacaaga attgtagggc
     2701 cttgtcttgg cagctcgaga agccacttgc ctttatcgtt acgagcggca tgtcctccag
     2761 gatgagcggg cattatcgcg catcgtttct ggcgctgcac caacggcgaa ccatcagatc
     2821 ttgaacgcct gtaaaatcaa ccactagcgc tgcgcgattt tcttggcgag gtaagccgag
     2881 aaatagcccg cataacgctt ttgtaattcc gcacactgcc agtctttgcc ggtcaacgtt
     2941 ccccggcaat cgggcgctcc gcatttgcat tcgatggaat aatcgtcgtc atcggtcgta
     3001 gcccaatcgt gagtgagttc ttcaccaccg cggatatcgc gcatggcgac aaacgtgatc
     3061 tcgccgcgca tgccgatgtt cgggtcgcac gaatggttgc tgtaaag
//
LOCUS       DASQUQ010000031         5871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_276528, whole genome shotgun sequence.
ACCESSION   DASQUQ010000031 DASQUQ010000000
VERSION     DASQUQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276528"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..700
                     /locus_tag="VE486_00870"
     CDS             8..700
                     /locus_tag="VE486_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZA37670.1"
                     /translation="MEASAGTRILVIEDERDVVDLLTLNLRKAGFAVSTATDGGTGLQ
                     RARSEKPAFIILDLMLPKMPGLEVCKVLKSDPATRQIPIMMLTAKAEEIDRIVGLEFG
                     ADDYVTKPFSPREVVLRIKAVLRRAAVKEEGEHLAVGPITIDAARHEVSVNGKRVNLT
                     SLEFKLLRTLMQRRGRVQARDRLLNDVWGYESVIDTRTVDTHVRRLRKKLGKAADVIE
                     SVRGFGYRLREH"
     gene            707..1984
                     /locus_tag="VE486_00875"
     CDS             707..1984
                     /locus_tag="VE486_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZA37671.1"
                     /translation="MSWLFLGLICIVFAAVGVVAWRKWIAPWRQIERLVTQVAGNDRP
                     RTFLVDGGAEPRRVGLALESIFARQQELNRQIAGRESGTQIILGAMQDGLLVVDRSLR
                     VTLVNQAFRKWFGLPEVSLGTPLLDIVRDATVDRLIAEMLRTGQAMQSELILTDSKTN
                     SERDVEVSAVPMSDDADLTTGAVVLFHDITQLKQADRVRRDFVANVSHELRTPLSILS
                     GYIETLLDSPKTSHEALSRILQVMERHSKRLGLLVDDLLSLAQLESRNTTLQLSDVQL
                     EELFESVIRDWGKQLAEKQLKVTVDFSPDARTIRADETRLHEVLYNLLENAVKYSREN
                     GEIRMQAAPRDHEIVLSVTDNGIGISKDDLPRIFERFYRADKARSRELGGTGLGLAIV
                     KHIVQLHGGRVEAESELGRGTTIRVVLPTRAAQ"
     gene            2080..3099
                     /gene="pstS"
                     /locus_tag="VE486_00880"
     CDS             2080..3099
                     /gene="pstS"
                     /locus_tag="VE486_00880"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZA37672.1"
                     /translation="MKSLKFSRKILAIALVTGVSATASAQMLINGAGATFPYPIYSKW
                     FDEYTKVDHSVRFNYQSIGSGGGQKQILAQTVDFGASDGPMSDENLAKAPGKLLHIPT
                     VAGAVVLTYNLPGNPGLKLDGETIADIFLGKIKKWNDSKIAALNSGASLPDKEIVVVH
                     RSDGSGTTFIFTDYLSKVSSEWKEKVGNNTSVNWPTGIGGKGNEGVAGQVKQTPGALG
                     YVELIYATQNKMPYAEVKNSAGEFVKPTLESVTAALAAAEIPDDFRFSMTNAPGKDAY
                     PIAGATWLLVYQQQKDAAKGKKLVEFLKWALTDGEKMAKDLEYAPLPESVQQRVLKQI
                     GEIKM"
     gene            3111..4103
                     /gene="pstC"
                     /locus_tag="VE486_00885"
     CDS             3111..4103
                     /gene="pstC"
                     /locus_tag="VE486_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZA37673.1"
                     /translation="MEIAIPPAVAPDDSRSVARAMAPPSRFGDKAFEWLTLAMAMAVI
                     LLVILTGWQLWRGSSLAVEKFGFHFLTTSTWDPVTEEFGALPFIYGTLVSSLIALLIA
                     VPLSIATAVYLTELAPLWLRQPIISLIEMLAAIPSVILGLWGIFVMIPWLRDYPFPLL
                     KRLFGWTPFFSGPIYGTSMLAGGIIIAIMILPIITSVSREILRSVPDLQREAAYALGA
                     TRWEVTRIAVLSYAKKGLFGAVILGLGRALGETMAVTMVIGNTPQIAASLFKPGYTLA
                     SVIANEFTEATTDLYLGALFEIGLVLFGVTILVNLIAQLLLKTMTTTSATRAVQ"
     gene            4100..4966
                     /gene="pstA"
                     /locus_tag="VE486_00890"
     CDS             4100..4966
                     /gene="pstA"
                     /locus_tag="VE486_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZA37674.1"
                     /translation="MTMNSDAADNHQWRKVKNALASAATFISAVLVIAPLGLVFFHLL
                     VNGASSVNWDFFTHLPKPVGEAGGGMVNAIVGSLELLALASVIGIPIGVLGGVYLAEY
                     GTARTNWVLRFVADVLNGVPSITWGVVVYGLVVLRFKGFSVYAGGLALGLIMIPLILR
                     TTEEVILLVPNGYREAALALGVARWKTIVHIVMKTASKGIITGILLALARVGGETAPL
                     LFTAFGNRFWNHNLGEPIAALPLQIFTYAISPYDDWHRQAWAGALVLVTGIFCVNVVV
                     RILTRGRTASVV"
     gene            4995..5828
                     /gene="pstB"
                     /locus_tag="VE486_00895"
     CDS             4995..5828
                     /gene="pstB"
                     /locus_tag="VE486_00895"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZA37675.1"
                     /translation="MANSDPTVVERAEKPAEPPPTSETTPPLTFEVKKLSIWYGEKRA
                     INDVTLDIASNAVTAIIGPSGCGKSTFIRCLNRMHELVPKTRIEGSVLLNGDDLYSST
                     VDPAIVRRRVGMVFQKSNPFPTMSIGENVVIGLRLNGVRDQKFLNERLEKSLRMAALW
                     DEVKDDLHKSGTSLSGGQQQRLCIARALAVQPEVLLMDEPCSALDPIATAKIEELIHE
                     LKTQYTIVTVTHNMQQAGRISDYTAFLYLGKLIEFGPTTKIFTNPSERQTEDYITGRF
                     G"
BASE COUNT         1283 a         1569 c         1724 g         1295 t
ORIGIN      
        1 gctgcgaatg gaagcgtcgg ccggtacacg gattttggtt atcgaagacg aacgtgacgt
       61 cgtcgatctt ctcaccctca acctgcgcaa ggcaggcttt gccgtctcga cggcaaccga
      121 tggcgggact ggattgcaga gagcgcgcag cgaaaagcca gcttttatta ttctggatct
      181 gatgctgccg aaaatgccgg gactggaagt ctgcaaagtt ttgaaaagcg atcctgcaac
      241 gcgccagatt ccgattatga tgctgacagc caaggcggaa gagatcgatc ggatagtggg
      301 actggaattt ggcgccgacg attatgtaac caagccgttt agcccgcgcg aagttgtttt
      361 gcggatcaag gcggttttgc gccgggccgc tgtgaaagaa gagggagaac atctggcggt
      421 cgggcccatc acgattgacg ctgcgcgcca tgaagtttcg gtcaatggca agcgggtcaa
      481 cctcaccagc cttgaattta agcttttgcg caccctcatg cagcgacgcg gccgcgtcca
      541 ggcgcgcgac cgtctgctta acgacgtctg gggttacgag agcgtgatcg acacgcgcac
      601 ggtggatacg catgtgcggc gcttgcgaaa aaaactcggc aaggccgccg atgtgatcga
      661 aagcgtgcgc ggcttcggtt atcgtttgcg ggaacattaa agcgcgatgt cgtggctgtt
      721 tctcggtcta atctgcatcg tcttcgcggc ggtgggagtg gtcgcctggc gaaaatggat
      781 cgcgccgtgg cgacaaattg agcggctcgt cacgcaggtt gccgggaacg atcggccgcg
      841 cacctttctg gttgatggtg gcgctgaacc gcggcgcgtt ggcctggcgc tggaaagtat
      901 tttcgcgcga caacaggaac tcaatcggca aatagcagga cgcgaatccg ggacgcagat
      961 aattcttggc gcgatgcagg atggcctgct cgtggtcgat aggagcctgc gcgtgacgtt
     1021 ggtgaaccag gcttttcgga aatggttcgg tctgccggaa gtctcattag ggacaccgct
     1081 tctcgatatt gtccgcgatg ccacagtgga tcggctgatc gcggaaatgt tgcggaccgg
     1141 ccaggcgatg cagagcgaat tgattctcac cgattcgaaa actaattccg aacgcgatgt
     1201 ggaagtcagc gccgtcccaa tgagcgacga tgccgatctg acaaccggcg cggtggtcct
     1261 gtttcacgac attacccagc tcaaacaagc cgatagggtg cggcgcgatt ttgtggcgaa
     1321 tgtttcgcac gaattgcgca cgccgttgtc gatcttgagc ggctacattg agactttgct
     1381 cgatagtccc aaaacttctc acgaagcgtt atcgcgcatc ctccaagtga tggaacggca
     1441 ttccaagcgg ctcggattgc tggtcgatga cttgctcagc ctggctcaat tggagtcacg
     1501 taatacgacc ttgcaattga gcgatgtcca attggaggaa ttgttcgaga gcgttattcg
     1561 cgactgggga aaacagttgg cggagaagca gctgaaagtg acggtcgatt tttcaccgga
     1621 tgcgcgaacg atccgcgcgg atgagacgcg gctgcatgaa gttctctata atttgctgga
     1681 gaatgcggtg aaatattcgc gcgaaaacgg tgaaattcgc atgcaagccg cgccgcgcga
     1741 ccatgaaatc gtactcagcg tgaccgataa cgggattggg atcagcaaag atgatttgcc
     1801 tcggattttc gagcgcttct accgcgccga caaagcccgt agccgcgagc tcggtggaac
     1861 cggccttggg ctggcgatcg tgaagcatat cgtccaattg cacggcggcc gcgtggaagc
     1921 cgaaagcgag cttgggcgag gaacgacaat ccgcgttgtg ctgccgaccc gcgcagcaca
     1981 ataacgtctg aggagcgtgc gccctcccca gatcggtgtc acacaaacgt aacactaaac
     2041 tttttgacgc ctcagtacgc gctgcagcag gctgaaacta tgaaatctct caaatttagc
     2101 cgcaaaatcc tggctatcgc gctcgtaacc ggagtcagtg cgactgcctc ggcgcagatg
     2161 ttgattaacg gcgcaggcgc aacgttcccc tatcctatct attcaaagtg gttcgacgag
     2221 tacacgaagg ttgatcattc tgtccgcttt aattatcaat cgattgggtc gggcggcggc
     2281 caaaaacaaa tcctcgctca gaccgtcgat ttcggtgcat cggatggtcc gatgagtgac
     2341 gaaaatctgg cgaaggcgcc gggcaaactc ctgcacattc caacagtcgc gggcgcagtg
     2401 gtcttgacgt ataaccttcc tggtaatccg ggactgaagc tcgatggcga aaccatcgcg
     2461 gacatcttcc ttggtaaaat aaaaaaatgg aacgactcaa aaatagccgc tctcaattcc
     2521 ggggccagtt tgcccgacaa ggagattgtt gtcgtgcacc gttctgacgg cagcggcacc
     2581 acctttatct tcaccgatta cctgagcaaa gtgagctcgg agtggaaaga gaaggtcggc
     2641 aacaatacat cggtcaactg gccgaccggc atcggtggca aaggcaatga aggcgtggcc
     2701 ggccaggtga agcaaacgcc cggcgcactc ggctacgtag aattaatcta tgctacgcaa
     2761 aacaagatgc cgtatgccga ggtcaagaat tcagctggtg aatttgtgaa accgacgctc
     2821 gaatccgtta ccgcggcgct ggcggcggcg gagattccgg acgacttccg tttctcgatg
     2881 acgaacgccc cgggcaagga tgcgtatcca atcgccggcg caacctggtt gctggtttac
     2941 cagcaacaga aggatgcggc caaaggaaag aagcttgtgg aatttctgaa atgggcgctg
     3001 acggatggcg aaaaaatggc caaggatctc gagtatgcac ccctgccgga aagcgtccag
     3061 cagcgcgtcc tgaaacagat cggcgagatc aagatgtaag ctgagcgcgc atggagattg
     3121 ccatacctcc agcagtcgca ccggatgatt cgcgatcggt agcgcgcgcg atggcgcctc
     3181 cctcgcgctt cggggacaag gcgttcgagt ggctgaccct ggcgatggcg atggcggtga
     3241 ttctgctcgt cattctgacc ggttggcagc tctggcgtgg atcatcgctc gcggtcgaga
     3301 aattcgggtt ccactttctc accacatcca cctgggaccc ggtcacggag gaatttggcg
     3361 cactcccgtt tatttacgga acgctggttt cttcgctgat tgcgctgctc attgctgtcc
     3421 cattgagcat cgcgaccgcg gtttatctga cggaacttgc gcccctctgg cttcgccagc
     3481 cgattatttc gttgatcgaa atgctggcgg cgattccaag cgtaatcctg ggattgtggg
     3541 gcatctttgt catgatcccg tggttgcgtg attatccgtt tccattgctg aaacggctgt
     3601 tcgggtggac gccattcttc agcgggccaa tctacggaac cagtatgctg gcgggcggaa
     3661 tcatcatcgc cattatgatt ttgccaatca tcacgtcggt ctcgcgcgag attctgcgca
     3721 gcgttcccga cctgcaacgc gaggccgcct acgcattggg tgcgacacgc tgggaggtaa
     3781 cccggattgc cgttctgagt tacgcgaaga aaggcttatt cggcgcggtc attctcggtc
     3841 tcggacgagc gcttggcgaa acgatggcgg tcacgatggt catcggcaac acgccgcaga
     3901 tcgcagcctc actttttaag cctggctaca cgctggccag cgtgatcgct aacgagttca
     3961 ccgaagccac gaccgacctt tatcttgggg cgttgttcga aatcgggctg gtgctgttcg
     4021 gcgtcacgat tctggtgaac ctgatcgcgc agctccttct caaaaccatg acgacgacat
     4081 cggcaacacg tgccgtgcaa tgacgatgaa ttccgacgca gcagacaacc atcaatggcg
     4141 caaggtcaag aacgcgctgg cttcggcagc cacctttatc tctgctgtgc tggtgatcgc
     4201 gccgctgggt ttggtctttt ttcatcttct cgtcaacggc gcgagctcag tgaactggga
     4261 ttttttcacg catttgccaa agcccgtcgg tgaagcaggc ggtggaatgg tgaacgcaat
     4321 cgtcggctcg ctggaactgc tcgcactcgc gtcggtcatt ggaattccca ttggtgtgtt
     4381 gggcggggtt tatctggcgg aatacgggac agcccgcacg aattgggtgc tgcgtttcgt
     4441 cgccgacgtg ctgaacggcg tcccgtcgat cacatggggc gtcgtcgttt acggtctggt
     4501 ggtgctccgt ttcaaaggct tctctgttta cgccggcggc ctcgcacttg gcttgatcat
     4561 gatcccactg attctgcgca cgactgaaga agtgattctg ctggtgccaa acggatatcg
     4621 tgaggctgcc ctcgcgctcg gtgtcgctcg ttggaaaacc atcgtgcaca tcgtaatgaa
     4681 gactgcctcc aaggggatca tcacgggcat tctgctggcg ttggcacggg tcggcggcga
     4741 aaccgcgccg ctgcttttca ccgcgttcgg caacaggttt tggaaccaca acctgggtga
     4801 acccatcgcc gcgctcccgc tgcagatttt cacttacgcc atctcgccct acgacgattg
     4861 gcatcgccag gcctgggccg gcgccctcgt gctggtcaca ggaatttttt gcgttaatgt
     4921 cgtggtaaga atcctgactc gcgggcgaac agcatcggtc gtctagagtt ctttgacaaa
     4981 tggttaattt acttatggca aattcggatc caacggtggt cgagcgtgcg gaaaagccgg
     5041 ccgagcctcc cccaacctcc gagactacgc caccgttaac tttcgaggtg aagaagcttt
     5101 cgatctggta cggtgagaaa cgagccatca atgacgtgac gctcgatatc gcgtcgaacg
     5161 ctgtcacagc gatcattgga cccagcggtt gcggaaaatc aacattcatt cggtgtctca
     5221 accgaatgca cgagctggtg ccgaagacgc gtatcgaggg aagcgtgctc cttaatgggg
     5281 acgatctcta ttcgagtacc gtcgatccgg ccatcgttcg ccgccgggtc gggatggtct
     5341 tccaaaaatc aaatccgttt ccgacgatgt cgattggcga aaatgtcgtc attggcttgc
     5401 gattgaacgg cgtgcgggac caaaaattcc ttaatgagcg tttggaaaaa tcgctccgca
     5461 tggccgccct ttgggatgaa gtaaaagacg atttgcacaa atccgggaca agcctttccg
     5521 gtggtcagca gcagaggctt tgcattgccc gcgccctggc ggtgcaaccg gaagtgcttt
     5581 tgatggatga gccctgttcg gctcttgatc caatcgccac ggccaagatc gaggaattga
     5641 tccatgagct caaaacccaa tacacgatcg taacggtaac ccacaatatg cagcaagccg
     5701 gacgcatctc cgattacacc gctttccttt atctcggaaa gctgatcgag tttgggccaa
     5761 ccacaaaaat tttcacaaat cccagcgaac gacagacgga agactatatc actggccgat
     5821 ttggctgatg ttgtgaggca agcgcttcgc ttgcggagca agagcaggag t
//
LOCUS       DASQUQ010000032         6749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_635999, whole genome shotgun sequence.
ACCESSION   DASQUQ010000032 DASQUQ010000000
VERSION     DASQUQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635999"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VE486_00900"
     CDS             complement(<1..393)
                     /locus_tag="VE486_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZA37676.1"
                     /translation="MNRIPFVSILLFASNFLCAQTPPPAPSPSPKESPPATVEYDIRW
                     GAKIPMRDKVELNATLYLPKTKDQTPSKTPVIFTLTPYISDTYHSRAAYFASHGYAFA
                     LVDVRGRGNSSGEFEPFANEARDGYDVVE"
     gene            complement(466..1128)
                     /gene="rpe"
                     /locus_tag="VE486_00905"
     CDS             complement(466..1128)
                     /gene="rpe"
                     /locus_tag="VE486_00905"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZA37677.1"
                     /translation="MQKVIVAPSILAADFARLADELHRVEQAGADWIHCDIMDGHFVD
                     NISFGPEIVGIVRKETNLPLDVHLMIQTPDHFAPRFVKAGANSITVHVEPEANHHVEK
                     TLRAIRDAGCRPGLSLNPATPFESVEPFLDKIDLLLVMTVHPGFGGQSFRREMMEKVK
                     RASEWNKTHERNIDIEVDGGINPHTARLSIQNGANVLVAGTSIFRAKDYAKAIRDLRG
                     SI"
     gene            1347..2384
                     /locus_tag="VE486_00910"
                     /pseudo
     CDS             1347..2384
                     /locus_tag="VE486_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
     gene            2484..3287
                     /gene="mreC"
                     /locus_tag="VE486_00915"
     CDS             2484..3287
                     /gene="mreC"
                     /locus_tag="VE486_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZA37678.1"
                     /translation="MSRTNIIALLIFGAILGYFLTFGADTTRKFKAGVYQLLAPFLTS
                     GSGLRKQITSVRTGLKSLDQLERENAALQVENRALRATNQGLRDIEHEVNRLRHALNY
                     RERSVFKLIAAEVVARDSSTWWRTVTINRGKSDGIETDMPIVTDVGLVGKTTTVSDSI
                     SVVLLVSDENCKVAASVEGTREQGIVSGERVTTGLNPLLNLNFLSKQADLKPGQKVYT
                     SGVGGVFPSGLLIGVVKIFRVRELDGQAQLTPAVDLSHLEDVFVVTGRR"
     gene            3284..3826
                     /locus_tag="VE486_00920"
     CDS             3284..3826
                     /locus_tag="VE486_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37679.1"
                     /translation="MIFFFLLLVLVLIAQIAEFFLPALPWLYNAHIYIVPVIVFYGAM
                     ALPFPLMLALALYAGLLLDALTVQVIGGKVEISMGSSILLYGVLAGLMHGLRPLFVRG
                     RWEVHCILSGICTSLIVLAQYLMITFRRGSLVFTPAIWWQIAAPGLIAMAIAPILFWF
                     LQWMGRMTGYSTFPERSDFE"
     gene            3823..6087
                     /gene="mrdA"
                     /locus_tag="VE486_00925"
     CDS             3823..6087
                     /gene="mrdA"
                     /locus_tag="VE486_00925"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZA37680.1"
                     /translation="MNRRRLHSRLRIQFLGLLMLMAMCALGLKLWWIQVAHGAEWTAQ
                     LRGSSQATVRIPSVRGEIKDRNGLTLVQNRASYEVDFYLPEMVKGYRERFGPPPLTEY
                     RAIISGMPKDQKEPDIIKIVNDGVIPRLNDLDLARDYNASKLQRHYRNDTEVPFSYIK
                     DIDFPTMAKFSEHDVGLPGVDIAIKPVRSYVYGALAAHVLGYVGAPDDIDKEDAKKFT
                     FYQGDMEGKSNVEKAVDEYLRGKPGVRYLRRNAKGAIDGVLREDPPKQGANVYLTIDA
                     RIQAIAEEALRAVGRGAAVVVDPNNGDILAMASVPSFDPNTFVPSIKAKDWEVLQKDE
                     SNPLVNRAVSAFPPGSTFKLITSLAGLRRGLAKTKFTCYGGVSYGDHYFQCWVSGKGY
                     THGTLTLADAIKVSCNAFFYQYGNAAGIDSIDRVGELLGLGQPSGLNLTGEQPGVLPG
                     PDWMKIHYPQERWSQAYTANVSIGQGYDLCTPLQMAMAYSTIANRGTSYYPRLVKKVL
                     NQDGTPVLDENGKIAVPEEPKVHADFRSEISPEDMELERRGFWKVVNESGGTGGRARL
                     SNVQVAGKTGTAQAKYHGREDTIAWFVCWAPFDQPKYTVAVMVQGGEHGGSVAGPVAS
                     RIMERSLALDEGKFDMQVAWLAPARKANPFQMLKDVGYRDSALSLGGEDEENANASQT
                     ADAQMAGAGAAPDVEPEADSQGQVRRRGAVPVARAVPAATPRPRNFFQRLFGPRRRPT
                     PPPQPPPTRRRGAR"
     gene            6160..>6749
                     /locus_tag="VE486_00930"
     CDS             6160..>6749
                     /locus_tag="VE486_00930"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HZA37681.1"
                     /translation="MIDKVKKILGLSSRKTGNKLILSVEKLESRVALLENGWLEEYSV
                     ERKDSRNIVGSVYKGKVKNIEMGLKAMFVDIGFEKNAFLHFWDAIPAALDNGIEEIDR
                     PANKRPRKRITAKDIPNIYPVGSDVIVQVTKGPIGTKGPRVTTNLSFAGRYLVLMPFS
                     DHSGISRKIENPKERERLRKILRELDVPEGIGVIIRT"
BASE COUNT         1536 a         1775 c         1971 g         1467 t
ORIGIN      
        1 ctcaacgaca tcgtatccat cgcgtgcttc attggcgaat ggctcgaact cgccgctgga
       61 gtttccgcgt ccacggacat cgacaagcgc aaatgcataa ccgtgcgatg cgaagtatgc
      121 cgcgcggctg tgatacgtgt cggagatgta gggcgtcaga gtaaatataa ctggcgtctt
      181 tgagggcgtt tggtcttttg ttttcggtaa ataaagagtc gcgtttagct cgaccttgtc
      241 ccgcatcgga attttcgcac cccagcggat atcgtattcg acagtggcgg gcggagattc
      301 tttgggagaa ggcgacgggg ccggtggcgg tgtctgcgcg cagagaaagt tcgacgcgaa
      361 caggagaata gagacaaacg gaattcggtt catgggccga ttgaaccacg aaccttgatc
      421 gcgacaagga agtttcgcgc tgggcggccc cgggatgaca agcagctaga ttgatccgcg
      481 caagtcgcga atcgcctttg cgtaatcttt cgcgcggaaa atcgatgtgc cggctacgag
      541 cacgttggcg ccattttgga tcgataatcg cgccgtgtgg ggattgatgc cgccgtccac
      601 ttcgatgtcg atgttacgtt cgtgagtttt gttccattcc gacgcgcgtt tcactttttc
      661 catcatttct cgccggaacg attgcccgcc aaaaccagga tggaccgtca tgacaagcaa
      721 aagatcgatc ttgtcgagaa acggctccac cgattcgaat ggcgtcgccg gatttaagga
      781 aagaccgggg cggcagccag cgtctcgaat cgcgcgcaag gttttctcga catggtggtt
      841 cgcttctggc tccacgtgaa ccgtaatgga attagcgcct gctttaacaa agcgtggcgc
      901 aaagtggtcg ggggtttgga tcattaaatg cacgtcaagg ggcagatttg tttctttgcg
      961 tacgatgccg acaatttccg gaccaaagga aatgttgtcg acaaaatgcc catccataat
     1021 gtcgcaatgg atccaatccg cccccgcctg ttccacgcga tgaagttcgt cggcaagccg
     1081 cgcaaaatcc gcggcaagga tggaaggggc aacgatgacc ttttgcatgg agtgatggag
     1141 taaagaagta atggaggaat ggtaataatc caacactcca ccactccaac gctccattac
     1201 tctgataata tgggcttgac gacccgaggc tctgtctcat gttacggcgt ttcagccggt
     1261 cgaatcgcgc tcgcgggaag tgcctgcgaa gatcgacata tgacgcgctt ccctcggact
     1321 ggaaagtgct tccaatttaa taactcatgt tcgacggact tttcggctgg ttatccagcg
     1381 atatcgggat cgatctcggc acggccaata ccctggttta tgtaaaagac cagggcatcg
     1441 tcttacgcga gccgtaagta gtagcggtgc aagccggcac caacaaagtg ctggcggtag
     1501 gggacgaggc caaacgcatg ctgggtcgca cgccggcaaa catcgttgcg gtgcgcccgc
     1561 tgaaagatgg tgtcattgcc gactttgaag taaccgaggc gatgctgcgg cattttataa
     1621 cgaaagtgca caatcgccgg gttcgggtaa gaccgcgcgt tgtgatcgca gtcccgtccg
     1681 ggattaccga agtggagaaa cgcgcggtgc gtgaatcggc aacacacgca ggagcacggg
     1741 aagtttatct aatcgaggaa ccgatggcgg ctgcaatcgg ggtgggactt cctgtgcagg
     1801 atcccgctgg caacatgatc atcgacatcg gcggcggcac gacggaagtc gcgctgattt
     1861 cactttctgg aattgtgttt agccgcagcg tgcgggtcgc cggcgacgag ctcgatgaag
     1921 cgatcgtgca gtacatgaag cgcgcttaca atttgatggt gggcgaacgc accgccgagg
     1981 agatcaaaat taagattggc tcggcctacc cgagtgaaaa ggagaccagt gtggaggtca
     2041 aagggcgtga tcttgtcgcc ggactgccta aaacactcat gattacttcg caggaagtgc
     2101 gcgaagcatt gcttgagccg atctcaacga tcgtcgattc cgtccgcatc acgctggagc
     2161 gctgtccgcc ggaactctca gcggacctcg tcgatcgtgg gctggtcctg gcaggcggtg
     2221 gcgcattgct gcgcggcttt gacaagctct tgagcgagga aacaggtttg ccggtccatg
     2281 tcgctgaaga tccactgagc gccgtggcgg aagggactgg caaatgcctc aatgaactta
     2341 aatttttgcg tcaagtggcc tcggcagatc gccagtggcg gtagcgattt tcgattttcg
     2401 gttgccgatt gccgattgaa attgacgagt cggcgctgcg gaattcgaaa ttcgacattc
     2461 ggaaaccgaa aaccggacat gcgatgagcc gcaccaatat catcgcgctg ctgatttttg
     2521 gcgccattct cggatatttt ctcaccttcg gggcggacac cacgcgaaaa ttcaaggccg
     2581 gcgtgtatca gttgcttgct ccgtttctga ccagcggctc gggactgagg aagcagatta
     2641 cgtcggtccg caccggccta aagtcgcttg accaattgga gcgcgaaaat gctgcgctcc
     2701 aggtggagaa tcgcgccctg agagcgacca accagggctt gcgcgacatc gagcatgaag
     2761 ttaatcgcct gcgccacgcg ctcaattatc gcgaacgatc ggtctttaaa ttgatcgcgg
     2821 ctgaagtggt ggcgcgcgat tcctcgacgt ggtggcgaac ggtgacaatc aaccgcggga
     2881 aaagcgacgg catcgaaacg gatatgccca tagtaaccga cgtgggcctg gtgggaaaaa
     2941 caaccaccgt aagtgacagc atctcggttg tccttttggt ttcggacgaa aattgtaaag
     3001 tcgcggcctc ggtggaagga actcgcgagc aagggatcgt ctccggcgag cgcgtgacga
     3061 cggggctgaa tccgctcctg aatctaaatt tcttatcgaa gcaagctgat ctgaagcccg
     3121 ggcagaaagt ctatacctcg ggagtgggcg gcgtttttcc ctccggcttg ctcatcggcg
     3181 tcgtcaaaat ctttcgggta cgagagttgg atggccaagc gcagctcacg ccggcggtcg
     3241 atctctcgca tctggaagat gtcttcgtgg tcactggccg aagatgatat tcttttttct
     3301 cctgctggtt ctggtgctga ttgcgcagat tgcggagttt ttcctcccgg cgttgccctg
     3361 gctttacaat gcgcatatct acattgtgcc tgtaattgtc ttttatggag caatggcgtt
     3421 gccctttccc ctaatgctag cgctggccct atatgcgggg ctccttctcg atgcgcttac
     3481 cgtgcaggtg attggtggaa aggttgaaat ctcaatggga tcgtcgatac tgctgtatgg
     3541 ggtgctggcg ggattaatgc atggcttgag gccgctcttc gtccgcggcc gttgggaagt
     3601 gcattgcatt ctgagcggta tctgcacgtc gttgatagtc ctggcgcaat acctaatgat
     3661 cacattccgg cgcggttctc ttgtttttac ccctgcaatc tggtggcaaa tcgccgctcc
     3721 aggcctcatc gcgatggcga ttgcaccgat cctgttttgg ttcctgcaat ggatgggacg
     3781 gatgacgggg tactcgacat ttcctgaaag gagcgatttc gaatgaaccg gcgccgcctt
     3841 cattcgcgtt tgcgtattca gtttctcggg cttctcatgc tgatggccat gtgcgccctt
     3901 ggcctgaagc tttggtggat acaggttgcg catggagcgg aatggactgc gcaacttcgg
     3961 ggcagttctc aagcaaccgt acggatccca tcggtgcgtg gcgagatcaa agaccgcaat
     4021 ggactcacac tggtccaaaa ccgggccagc tatgaggtcg atttctattt gccggaaatg
     4081 gtgaagggct atcgtgaacg cttcggtccc ccacccttga ctgagtaccg ggcgattatt
     4141 agcggaatgc cgaaagacca gaaagagcca gatatcatta agatcgttaa tgatggcgtc
     4201 atcccacggc tcaacgatct cgatctcgcg cgcgattaca atgccagcaa gctccagcga
     4261 cattatcgca acgacaccga agttccattt tcctacatca aggacattga tttccccacc
     4321 atggcaaaat tttcggagca cgatgtggga ctgcccggcg tggacatcgc catcaaaccg
     4381 gtgcgttcct acgtttacgg cgcactggcc gcacatgttc tcggttatgt cggcgcgcct
     4441 gacgacatcg ataaagaaga cgcaaaaaaa ttcacgtttt atcaggggga catggaaggt
     4501 aaatcgaacg tcgaaaaagc agtggacgaa tatctgcgcg gcaagcctgg cgtgcggtac
     4561 ctacgacgta atgcgaaggg cgcaatcgat ggcgttttgc gcgaggaccc gcccaaacag
     4621 ggagcgaatg tttacttaac catcgacgcc cgcatccagg cgattgccga ggaagcgctg
     4681 cgcgcagtgg gtcgtggcgc cgcagtggtt gtcgatccaa acaacggcga catcctggcg
     4741 atggcctcgg tcccctcgtt cgatcccaac acctttgtgc caagcatcaa agcaaaagat
     4801 tgggaggtac tccagaagga tgaaagcaat ccgttggtca accgcgccgt gagcgcgttc
     4861 ccgcctggat cgacattcaa gctaatcact tcgctcgccg gattacgccg cggcctcgcc
     4921 aaaacgaaat tcacgtgtta cggcggcgtt tcatatggcg accattactt tcaatgctgg
     4981 gtaagcggga aaggttacac tcacggcaca ctcacgctcg ccgatgccat caaggtgtcg
     5041 tgtaacgcgt tcttttacca atacggcaac gctgccggaa tcgattcgat cgaccgggta
     5101 ggcgaattgc tgggcctcgg tcaaccctcg ggtctcaacc tcaccggcga acaaccggga
     5161 gtgctgcctg gcccggactg gatgaagatt cattatccac aggaaagatg gtcgcaagct
     5221 tatacggcga atgtttccat tggccagggc tacgatctgt gcacgccctt acaaatggcc
     5281 atggcctatt caactattgc caatcgcggc acttcctatt acccgcgcct cgtcaaaaaa
     5341 gttttgaacc aggacggcac gcccgtcctg gacgagaacg gaaaaatcgc agtgcccgag
     5401 gaaccgaagg tgcatgccga ttttcgtagt gaaatttcgc ctgaggacat ggaattggag
     5461 cggcggggat tttggaaagt cgttaatgaa agcggtggaa cgggcggtcg cgcgcgcttg
     5521 agcaacgtgc aggtggcggg aaagaccggc actgcccagg ccaagtatca cggcagagaa
     5581 gatacaattg cctggtttgt ttgctgggcg ccgttcgatc aaccgaaata tacagttgcg
     5641 gtaatggtgc agggcggcga acacggcggc agcgtggccg gtccggtggc aagcagaatc
     5701 atggaacgct cactcgccct cgatgaggga aagttcgata tgcaagtcgc atggttggcg
     5761 ccggcgcgaa aggccaatcc gttccaaatg ctcaaggacg taggttatcg cgattccgca
     5821 ctaagtctcg gaggcgagga cgaagaaaat gccaacgctt cgcaaactgc ggatgcgcaa
     5881 atggcaggcg cgggtgccgc tcctgacgta gaacccgaag cggattcgca aggccaggtg
     5941 agacggcgag gcgccgttcc tgtcgctcgc gccgtcccgg ccgcgacgcc ccgtccgcgc
     6001 aactttttcc aacggctctt cggaccccgg cgtcgaccga cgcctccacc acagccgccg
     6061 cccacccgcc ggcgcggtgc acgatgaact ttcgatctga aacgccgccg ctgaacttcc
     6121 cggcagttcc gattcggatc gatcaacctc tccagtttta tgatagacaa ggtaaagaaa
     6181 attttagggc tttcttcccg caagaccggg aacaaactca tactcagcgt agaaaaactc
     6241 gagtcgcgcg tcgcgttgtt agagaacggc tggctcgagg aatacagcgt cgagcgtaaa
     6301 gactcgcgca atatcgtcgg cagcgtttac aaagggaaag tcaagaacat cgaaatgggg
     6361 ttaaaggcga tgttcgtgga catcggcttc gagaaaaatg cctttcttca tttttgggat
     6421 gctattcccg ctgcgctcga caacggaatt gaagaaatcg atcggcctgc aaataagcgg
     6481 ccgcgaaaac ggatcaccgc aaaagatata ccgaacattt acccggtcgg ctcggacgta
     6541 attgtccagg taaccaaagg accgatcggg acgaaaggac cgcgggttac gacgaacctc
     6601 agtttcgccg ggcgttatct ggtgttgatg ccgttcagcg atcacagcgg catctcacga
     6661 aaaatcgaga atccgaagga gcgcgagcga cttcgcaaga tcttgcgcga actggacgtt
     6721 cccgaaggga tcggcgttat catacgcac
//
LOCUS       DASQUQ010000033         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1272526, whole genome shotgun sequence.
ACCESSION   DASQUQ010000033 DASQUQ010000000
VERSION     DASQUQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272526"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..975)
                     /locus_tag="VE486_00935"
     CDS             complement(67..975)
                     /locus_tag="VE486_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37682.1"
                     /translation="MIRLLRDTGYWDDESAWRYYGDYENNYNTIGNQQSRPDAALVEK
                     LVNSVDARLMNECLVRGIDPESSDAPESIVEAVAILFEPGADPKSLSAGRIRNWPDAK
                     RNEVARGITFAAAGASPQQGNPCFTISDSGEGQTPDRMPETFLSLTKSNKLRIPFVQG
                     KFNMGGTGALKFCGRHNLQLVVTRRHPAITKLEHNADDQCGFTVVRREDPSRNRRSSV
                     YTYLAPVGCREKPGRGGVLRFNAESMPLFPEGRNAYSRPSTWGTLVKLYDYVGDRLKG
                     THFAQGRHAEPNGHFVAGRSAADSFS"
     gene            complement(1040..1441)
                     /locus_tag="VE486_00940"
     CDS             complement(1040..1441)
                     /locus_tag="VE486_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZA37683.1"
                     /translation="MKTVVLDASALLAMFFGEAGMEQVRSLFHKAADADRTLFIAAVN
                     WAEVLYKVEQKQGREGLATAKRFERTMPLEVAMLDRGLAESAAALKSDHDLGLADAFA
                     AALAKEKRAELVSGDLEFKPLEKEIKINWLK"
     gene            complement(1438..1704)
                     /locus_tag="VE486_00945"
     CDS             complement(1438..1704)
                     /locus_tag="VE486_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZA37684.1"
                     /translation="MKDNSMETSYVTSKGQLVVPSRIRRKYGIKPGTRVNFVEDGERI
                     IFQPVTREYIDSFCGIFKKKPGEKSVVEEHLQDRRKERDRENAK"
     gene            complement(1772..>3523)
                     /gene="mutS"
                     /locus_tag="VE486_00950"
     CDS             complement(1772..>3523)
                     /gene="mutS"
                     /locus_tag="VE486_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZA37685.1"
                     /translation="PADYVVLDAATQANLELVESRSARDTSLFAVLDRTVTPMGGRKL
                     RAWILQPLRNLTELTRRQQMIADLLQEPDLLGSIRAELKVIRDIERAAGRLSQASGNA
                     RDLVALKTSLKQIPALKVELAKLIERVTFGAAGPHTSHQSCATHLQNEIHEMPALAER
                     LGKALVNDPPLALKEGGIFRDSYDTDLDTLRQASREGKNWISHLQEREIAATGIKSLK
                     VRYNSVFGYFIEVTKSNLVNVPAHYTRKQTTVGGERFVTPELKEIEAKILGADERARQ
                     LEYQLFQQLRDETLRELEPIQQTAAAIAVLDVICALAETARLFRYCRPILNDTLQLVI
                     QDGRHPVLDQNLVEEKFVPNDTSLDGENLRLAIVTGPNMAGKSTYIRQVALIVLMAQI
                     GSFVPAESAEIGLVDRIFTRVGANDDLARGQSTFMVEMNETSNIVNNATERSLVILDE
                     IGRGTSTFDGLSIAWSVAEFLHDKIKARTLFATHYHELTKLAEERSGVCNFNVAVREW
                     NDQIIFLRKIVPGGADKSYGIQVARLAGLPKEILDRAKEILSHLEKPDGTAARPTERK
                     RSTKRMPQSEKPQLDLL"
BASE COUNT          698 a         1010 c          955 g          860 t
ORIGIN      
        1 cggacgctta agcctgtaag cgtggtttca aagctgccgg cgtggccgcg gtatccaggt
       61 cgacattcat gaaaacgaat cggcagcgct gcgtccggca acaaaatgtc cattcggctc
      121 agcatgccgt ccttgcgcaa aatgtgtccc tttaagcctg tcgccgacgt aatcgtacag
      181 cttaacaagc gtgccccagg tcgaaggcct tgagtacgca ttccgtcctt cgggaaatag
      241 tggcatgctc tcggcattaa atctgagtac gccgccacga ccgggttttt cgcggcagcc
      301 gactggcgca agataagtgt aaacagagct tcgccgatta cgtgagggat cttccctgcg
      361 cacaactgtg aagccgcact gatcatcagc gttgtgctca agcttcgtga tcgctgggtg
      421 gcggcgagtg acgacgagtt gcaggttgtg ccgcccgcaa aactttaatg cgcctgtgcc
      481 acccatattg aatttccctt gcacgaacgg gatgcggagc ttgttcgatt tcgtgagaga
      541 taaaaatgtc tcgggcattc tgtcaggcgt ttgtccttct ccagaatcgc taattgtaaa
      601 gcacgggtta ccttgttggg gcgatgcgcc tgcggccgcg aaggtaattc ctcgcgcaac
      661 ctcgttgcgc tttgcgtcgg gccagtttct aattcgtccg gcgctgaggc tctttggatc
      721 agctccgggc tcaaataaaa tagccaccgc ttcgacaata gactccggcg catccgaact
      781 ctccggatca attccacgga cgagacactc gttcatcaac cgcgcatcga cagaattcac
      841 cagcttttct accagcgccg catctggtcg gctctgttgg tttccgatgg tgttgtagtt
      901 gttctcgtaa tcgccgtagt accgccaggc gctttcgtca tcccaatatc cagtgtccct
      961 gagtaggcga atcacgctct cttcggaatc tgcttggatc agtgcgagac agagctgctt
     1021 aagcgcatca ttgtccatat tatttgagcc agttgatttt gatttccttt tccagcggct
     1081 tgaattccag gtcgccgctc accagttcgg ctctcttttc cttggcaagt gcagcggcga
     1141 aggcgtcggc caatcccaaa tcgtggtccg acttcaacgc cgcggcggat tccgccagcc
     1201 cacggtcgag catagccact tccagtggca tagtgcgttc gaagcgcttc gcagtcgcta
     1261 gaccctcgcg accttgtttt tgctccactt tgtagagcac ttccgcccaa ttcaccgcag
     1321 cgatgaacaa cgttctgtct gcgtcggcgg ctttatgaaa gaggctccgc acctgttcca
     1381 tgcccgcttc gccgaagaac atcgcgagta aggcggaggc gtcgagaacg acggttttca
     1441 cttggcattc tcgcgatcgc gctctttacg gcggtcctgt agatgctcct ctaccacgga
     1501 tttctcgccc ggcttctttt tgaaaatgcc gcagaacgaa tcgatatatt cgcgcgtgac
     1561 aggctggaag atgatccgct caccatcttc cacgaaattg acgcgggtgc ctggtttgat
     1621 gccgtacttt cgtcgaattc gcgacggcac caccaactgg cctttcgatg tcacatagct
     1681 cgtttccatt gagttgtctt tcatttaagt aatattatgg catcgctaag atagtaagac
     1741 aatatgtttt tgtaagagat tcggccggcg cttacagcaa atctagctgc ggcttctctg
     1801 attgcggcat gcgtttggtc gatcttttcc gttcggtggg tcgcgccgct gtgccgtctg
     1861 gtttctccag gtgcgagaga atctccttcg cccgatcgag aatttccttt ggcaaaccgg
     1921 ccagtcgagc gacctggatt ccgtagcttt tatcggcgcc gcccggcacg atcttgcgca
     1981 ggaaaatgat ctgatcgttc cactcgcgca cagcgacgtt gaagttgcag acgccgctgc
     2041 gttcctcggc gagtttggtc agttcgtgat aatgcgttgc aaacagggtg cgcgccttga
     2101 ttttatcgtg gagaaattcg gcgacgctcc aggcaatgga gagcccatcg aatgtcgagg
     2161 tgccgcggcc aatctcgtcg aggataacaa ggctgcgctc ggtggcattg ttgacgatgt
     2221 tcgaggtctc attcatctcg accatgaacg tcgattggcc ccgcgcgaga tcgtcgttcg
     2281 cgccaacccg ggtaaaaatg cgatcgacta agccgatctc cgcgctctcc gctggaacga
     2341 aactcccaat ctgcgccatc aatacgataa gcgccacctg acggatataa gtggatttcc
     2401 ccgccatatt tggcccggtc acgatggcca gtcgcaggtt ctccccatca agcgatgtgt
     2461 cgtttggcac aaatttttct tccaccagat tttgatcgag caccggatgc cggccgtctt
     2521 gaatgacgag ctggagcgtg tcgttcaata tcgggcggca gtaacggaaa aggcgcgccg
     2581 tctctgccag cgcgcagatc acgtcgagaa ccgcaatggc cgcggccgtc tgttggatcg
     2641 gctccagctc gcgcaaagtt tcgtcacgca attgttggaa aagttgatac tcgagctggc
     2701 gggcgcgttc gtctgcgccg aggatttttg cctctatttc cttgagctcc ggcgtgacga
     2761 aacgttcgcc gcccacagtc gtctgcttgc gcgtgtaatg cgcgggcacg ttcaccaggt
     2821 tcgatttcgt cacctcgatg aaataaccga agaccgagtt gtaacgcact ttgagcgatt
     2881 ttatgccggt cgcggcaatc tcgcgctcct gcaaatggct gatccagttt tttccctcac
     2941 gcgacgcctg ccggagcgta tcgagatcag tatcgtaact gtcgcggaaa attccgcctt
     3001 ccttcagcgc cagcggcgga tcattgacaa gcgctttccc caacctttcc gccagcgccg
     3061 gcatttcgtg gatttcgttt tgtagatgtg tcgcacacga ctgatgggac gtatgtggcc
     3121 cagccgcgcc aaacgtcact cgctcgatta gtttcgccaa ctcgaccttg agcgcgggga
     3181 tttgcttaag cgaagttttc aacgccacca gatcgcgtgc gttgcccgag gcctggctta
     3241 atcgcccggc ggcccgttca atatcgcgaa tcaccttcaa ttcggcgcgg atcgacccga
     3301 gcaaatccgg ctcttgcaac aaatcggcga tcatttgctg gcggcgcgtc aattcggtca
     3361 aattacgcag cggttgcaga atccaggcac gcagtttgcg gccgcccatc ggcgtgacag
     3421 tgcgatcgag cacagcaaac aggctggtgt ctcgcgcgct gcgggactca acaagttcaa
     3481 ggttcgcctg ggtggctgca tcgagcacga cgtaatcggc ggg
//
LOCUS       DASQUQ010000034        12383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1176910, whole genome shotgun sequence.
ACCESSION   DASQUQ010000034 DASQUQ010000000
VERSION     DASQUQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 12383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12383
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176910"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VE486_00955"
     CDS             complement(<1..242)
                     /locus_tag="VE486_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37686.1"
                     /translation="MKPIRICLWPTSVAIATAICLGTASAASSEHSVSTPQQVFDGMR
                     QSFQAKKAKGVHARYQWELSGPQGGQWWIDVNDGKY"
     gene            complement(426..1613)
                     /locus_tag="VE486_00960"
     CDS             complement(426..1613)
                     /locus_tag="VE486_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA37687.1"
                     /translation="MRWPSLQAIASVAYKEFLHIYRDRRVLLLVLILPLMFTLLFGHA
                     FETGELTGVKSLLIDRDNTASTQQLIDILLKNKTFAWRRGSPETRNESDLIGHRVRAT
                     LVIPAGWTEGLTNGNPKPLLLYLDGGDINTADAVEGNVRQSLGDFQLKRRDSMIEALP
                     EEVFDLGKQLPVQIRKQFVSIMEMWTVDDKILYNPKSRVIDYVIPGIIGLILQLLTVT
                     LMACTIARERESGTLYQLMVTSLQRSEIVIGKMLPYLVVSIFLVIVIMTVSRWHFGVA
                     FYQLPALAFICLLFLLCSLGLGLLISAFSHTQTQAIQFSVFFLLPVFVLSGAFAPLEQ
                     LPQAIQYVSEFFPLTHFCRAFRLVNMYHASVGFYAVDLIVLLAGALITFIGAAFLLKR
                     IEQ"
     gene            complement(1610..2560)
                     /locus_tag="VE486_00965"
     CDS             complement(1610..2560)
                     /locus_tag="VE486_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA37688.1"
                     /translation="MNKPVIVLNDLSKSFGDVHAVQDLSFTIAPGTIFGFLGANGAGK
                     TTTMRMLCGLTHPTGGDATIEGADVWRDRQLVRTKFGYVSQRFSLYRDLSVMENFRFF
                     GGAYRVPGAELDERIQRLLGAMDLKKKADVAAGSLSGGMRQLLALGCALVHDPPLLFL
                     DEPTSGLDPVHRQQIWNLLYDLSNRGKTIFVTTHYMDEAERCTEVGFIESGRLLAKAS
                     PRALKSSFRARLLEIDIEPLMPALVRLRDVPDLLGVSLRSGSLRLYAADSEKLMAQWR
                     KNWPFPDLRLLGERWVEPDMEDVFTAYSQGYDGILKEPAS"
     gene            2631..3683
                     /locus_tag="VE486_00970"
     CDS             2631..3683
                     /locus_tag="VE486_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37689.1"
                     /translation="MKFETMEPSRRLLTNASVLLLFLLGSLTTARGDAVSELASFSIF
                     DKVDLAQLAKSDVKTAHGPPMSNPRFLAVQSCYVAPRAPEQLIAALRRWDPTRHSELK
                     VFLHSDLPASPTAANFEKLKSAPNNGPVRTFVAETQKLSSDLQISRDEAKKFSAGGAG
                     GGGAMPASVVAFWTDVLVSRTKSFVSGGTSGQPPYDHSGKAIRVSDEVNGLLREQGKI
                     QRQFSGLLGATGIGRGAGSLRPQLYWELLDVDDHGVVTLGADYNRAGGGGSYQAADVL
                     YYASGGYYVALTLYQMWPVTVDGKASTLVWRGDMISAPSLASLHGVERLGSESVMMKQ
                     ISKAVTLFRRDTSGSR"
     gene            3688..4608
                     /locus_tag="VE486_00975"
     CDS             3688..4608
                     /locus_tag="VE486_00975"
                     /inference="COORDINATES: protein motif:HMM:NF039394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HZA37690.1"
                     /translation="MKSFARRFFLRSLCLASFFWLGASILAQESNPESFGDWFASGEY
                     VVEETYVGSGDVRRNPQKSVRDFDEHDSILHLVFTPRIKAGVLRLGAEWERFTFGIPD
                     GAPLPDTLQSVNLVIGLDTQLSDSILIRVEALPGVYNMGFDDLSDDFNAPFVAGGTYI
                     FTPDLQLVLGVSVDIERKYPVLPAAGVRWKMARQWVLNAVLPTPRLEFEMNSGVTLYV
                     GANLKQNNYRVEDHFGDNSGVPRLNHAVITYTEVRTGAGFDWKISPGVTLSGEVGYQP
                     YREFDFYRAHVRYSQDGGAPYGMVSLQGAF"
     gene            complement(4680..6095)
                     /locus_tag="VE486_00980"
     CDS             complement(4680..6095)
                     /locus_tag="VE486_00980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZA37691.1"
                     /translation="MPPPTDPRSYESPEGQRPAAVTPPIELERRAHDPYSAFRFGGFA
                     LFTAGNFLSITGRLMLAVAVEWEIYARTHSATALGLVGLVIAVPVVALSLPAGHLADR
                     FSRKHIILVSQIFSALASMALALVSWKHLSIPPIALLREGNHALAAIAAIFERHHPTF
                     HFDDASIPLIYLLVFIGAIARTFSWAARSSFFPTLVSRDAFANAVTWNNSVFQIGSVV
                     GPGVSGLLVAHVGFPFVYMLDALCAFFFFLLILPIRRSTQSRNQTEKGTWRSLVAGMR
                     FVFSKKVILATITLDLFAVLLGGATALLPIFADQILHCGPIGLGWMRAAPAVGAFAMA
                     LLVAHLPPMKRAGKTLLWCVTGFGIATILFGLSKAFWLSLALLFFIGAFDSVSVIIRG
                     SIVQLMTPDEMRGRVSAVNNIFIGTSNEFGALESGLTAALFGPVISVVAGGIGTILVV
                     LGVAWHWPQTRKIGALDRTLS"
     gene            6137..6508
                     /locus_tag="VE486_00985"
     CDS             6137..6508
                     /locus_tag="VE486_00985"
                     /inference="COORDINATES: protein motif:HMM:NF016718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37692.1"
                     /translation="MSGERGNPREGGDNGTLVGPEPAMPPGPEKVLKNLRIFLPFPET
                     AALKSAASGKMDNIIEAKELQIERKHFYVELRENDRGKFLRITEEAHGRRNSIIVPST
                     GVDDFTAAISEVLTNNGSAPQ"
     gene            6725..9460
                     /gene="ppdK"
                     /locus_tag="VE486_00990"
     CDS             6725..9460
                     /gene="ppdK"
                     /locus_tag="VE486_00990"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZA37693.1"
                     /translation="MQPNAAGKRTKTPRYVYYFGDGHADGSGSMKPLLGGKGANLHEM
                     TRIGLPVPPGFTITTEVCSYFYDHNRAYPPQLEAQVADALARVEKAVGKKLGDGERPL
                     LVSVRSGARDSMPGMMDTILNLGMNDKVVEIVAKKTNNARFAWDSYRRFLQMYGDVVM
                     GVQKRAGEDHEPFEMVIERLKDERYGKHDFPDVRLTVADLKELVQRFKALIKERTGKS
                     FPQDPTEQLWGAIGAVFGSWMNDRAIVYRRKYGIPHEWGTAVNVQTMVFGNMGKTSAT
                     GVAFTRDPATGEKVFYGEYLINAQGEDVVAGVRTPHPIADLAKEMPAAHKELMKVRAI
                     LERHFKDMQDLEFTVEENRLYILQTRNGKRTGHAAVRIAVDMVGEKLIPKKEAVRRIP
                     ADSLAHLLAPIFDRQAAREAKKIGSGLAAGPGAACGHVVFSAEEAVVRAEKGEKVVLA
                     RIETSPEDLRGMIAAEGILTSRGGVSSHAALVARQMGKVCVCGATGIHIDYQNRTLSA
                     NGTTLHRGDYISIDGTAGEVFAGEVATAPSEIVQVLVDRSLDPKKSVTYQEYAKLMKW
                     TDEFRTLGVRTNADTPEQVANAVAFGAEGIGLCRTEHMFFEGNRIDAMREMILAGTKD
                     ERGRALAKLLPYQRDDFIGIFTALKGLPATIRFLDPPLHEFLPSDHAQQSELANKLGV
                     TVEQIARRVHELHEFNPMLGHRGCRLGIVFPEISEMQARAIFEAAAEVQGSGIKVRPE
                     IMIPLVGFPRELKLQIDIVRRVATEVTKERQTKFSYLVGTMIEIPRAALVADQIARDA
                     EFFSFGTNDLTQTTLGMSRDDSGSFLPTYAELDIVDTNPFASIDQKGVGRLMEITCDL
                     GRKTRPDIKLGICGEHGGEPTSVKFCERLGLDYVSCSPFRIPIARLAAAQAAIES"
     gene            9465..10685
                     /gene="ilvA"
                     /locus_tag="VE486_00995"
     CDS             9465..10685
                     /gene="ilvA"
                     /locus_tag="VE486_00995"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HZA37694.1"
                     /translation="MATFQDIEAARKRIADAVYCSPCRRSIPLSELTEMEIFCKFDNL
                     QRTGSFKERGARNALAQLSPDQQKRGVIAASAGNHAQALAYQGKLLGIPATVVMPKFA
                     PLVKVSNCQKLGATIVLHGRDFSEAKEHALEVAKEKGLAYIDGYDDPAIIAGQGTMGL
                     EIIEQTPDLDVVVVPVGGAGLLAGVSLAIKTLRPNAAIVAVEAENVASFSAAVEAGKP
                     TRIAIHPTLADGLAIPQVGSNAFQIAKPLVDLTVTVTEEQIALGILRLVELEKSVVEG
                     AAATPLAACMSGKLKEFAGKRVVLLLCGGNIDPNVLSRVIERGLVADGRLGRFTAVIS
                     DRPGGLADLAAQIASIGASIKQVVHDRAFASPDVSAVNVLCTVETRNHQHLAELRALL
                     KSRGVKTYDSNNPL"
     gene            10779..>12383
                     /gene="gcvPA"
                     /locus_tag="VE486_01000"
     CDS             10779..>12383
                     /gene="gcvPA"
                     /locus_tag="VE486_01000"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZA37695.1"
                     /translation="MKLHERTTEFDSFVRRHIGPNQEEIDAMLAEVGFENLDALIDAA
                     VPRNIRLDRQLNLPEAKSEIDALAELRAISKKNKVARSFIGAGYYDCITPPVIQRNIL
                     ENPGWYTAYTPYQAEIAQGRLEALLNFQTMITDLTGFDIANASLLDEATAAAEAMALC
                     HAVVPDRKTFFVADNCHPQTIAVIQTRAKPLGIEIKIDNYSRFKFDDTVFGALVQYPA
                     TDGAIYDYSDLIKEAHNTGALVVVAADILALTLLKPPGEFGADVAVGNTQRFGVPLGF
                     GGPHAAYFATRDRYKRHMPGRLVGVSHDADGRPAYRLALQTREQHIRRDKATSNICTA
                     QVLLAVIASMYAVYHGPRGLRAIAECVHRQTSQLADGLRALGLKIAHENFFDTIRVEV
                     ESSDVILEHAEKAGCNLRALGSRAVCISFDEMTTVRDIELLMSIFRGTHVRDFADDNL
                     GEPPIRIPQSAIRTSDFLTHPIFNTHDTETEMLRYLKRLESRDLSLTTSMIPLGSCTM
                     KLNATAEMFPISWPEISRLHPFAPDEQ"
BASE COUNT         2886 a         3476 c         3430 g         2591 t
ORIGIN      
        1 ttgtacttcc catcgttcac atcgatccac cattgtccgc cttgcgggcc actgagctcc
       61 cattgataac gggcatgtac tccttttgcc ttcttggcct ggaaactttg gcgcatgccg
      121 tcgaacactt gctgcggcgt cgagaccgaa tgctcacttg aagcggcgct cgctgtcccc
      181 aggcagattg ccgtagcaat cgccacgctc gtcggccaca aacagattcg gatcggtttc
      241 acaacgccca tactcaaaaa gaagatcgtg cccgcgatga aaaacggctg tcacttaagg
      301 atgacgaagc acccgcctcc gctccgctac ggcgcggcag gcgaatgtcg aatgacgaag
      361 gaatgaagaa atccggccgg cgtccgtcat tcgttattcg tcagtcggat ttcgtcatgc
      421 ctgtctcatt gctcaattcg ctttagaaga aaggctgcgc cgataaaagt gatcaacgca
      481 cccgccagga gcacaatgag atcgacggca taaaatccca cgctggcgtg atacatgttc
      541 acgagccgaa acgcgcggca gaaatgagtc agggggaaaa actcggaaac atattgaatt
      601 gcctgaggta gttgctccag cggcgcgaac gcgcctgaga gaacaaaaac tggaagcaga
      661 aaaaaaacag agaattgaat ggcttgcgtt tgggtgtgcg aaaacgcgga gatcagcaaa
      721 ccgagaccaa gcgagcaaag caaaaaaagc aggcaaatga acgcgagcgc gggtagttga
      781 taaaatgcca ctccaaaatg ccatcggctc actgtcatga tcacgatgac caggaatatc
      841 gaaaccacca gatagggcag cattttcccg attacgatct cgctccgctg cagcgaagtc
      901 accatcaact ggtaaagagt acccgactca cgttcccgcg cgatcgtgca cgccatcagc
      961 gtgaccgtca gcaattgcaa gattaaaccg ataattcccg gaatcacata atcgatgaca
     1021 cgcgacttcg gattataaag aattttgtcg tcaaccgtcc acatctccat tatcgagacg
     1081 aattgtttgc ggatttgcac cggaagctgc ttgcccaggt cgaagacttc ttccggcaat
     1141 gcttcgatca tcgaatcgcg tcgctttagc tggaagtcgc ccaggctctg tcggacgttc
     1201 ccttccactg cgtccgcggt gtttatgtcg ccaccatcca gataaagcag caacggcttt
     1261 ggattgccat tggtcagacc ttcggtccac ccagccggaa tcaccagcgt cgcgcgcacc
     1321 cggtgaccga tcaaatccga ctcgttcctt gtttcaggcg atccccggcg ccacgcgaat
     1381 gttttgtttt tcaaaaggat atcgatcaac tgctgcgtgc ttgctgtgtt gtctcgatcg
     1441 atcaagaggc ttttcacccc tgttaattcg ccggtctcga aagcgtgtcc gaaaagcagg
     1501 gtaaacatca gcggcaaaat caataccagg agaagcaccc gccggtcgcg gtagatgtgc
     1561 aggaattctt tgtaagcaac actggcaatc gcttgaagtg atggccatct catgaagctg
     1621 gttctttcaa aatcccatcg tagccctggg aatatgcagt gaaaacgtcc tccatgtcgg
     1681 gttcgaccca gcgttcgccc aacaaccgaa gatcaggaaa aggccagttc ttgcgccatt
     1741 gcgccattaa tttttcggag tcagccgcat aaagccgcag actgccgcta cgcaatgaaa
     1801 ctcccagaag atcggggaca tctcgcaatc gaaccagcgc gggcatgagt ggttcgatgt
     1861 cgatctcgag caagcgagcg cgaaagctcg acttcaaagc gcgaggcgag gctttcgcca
     1921 gcagccggcc actttcgatg aagcctactt ccgtgcaacg ctccgcctca tccatgtaat
     1981 gcgtggtgac aaaaattgtc tttccccggt tgctcagatc gtagagcaag ttccagattt
     2041 gctgacgatg cacggggtcg agtccagagg ttggttcgtc aagaaaaagc agcggagggt
     2101 cgtgcaccag ggcgcaaccg agcgcgagta attgccgcat tcctccggac aaacttccag
     2161 cagcgacatc ggctttcttt ttcagatcca tcgcgcccag caatctctgg attcgttcgt
     2221 ccaactccgc accgggaacc ctgtaggcgc cgccaaaaaa acgaaagttc tccataacac
     2281 tgagatcgcg atacaggctg aatcgctgcg agacgtaacc gaacttcgtc cgcacgagct
     2341 ggcgatctct ccagacgtcc gcgccttcga ttgtcgcatc accaccagtg ggatgagtaa
     2401 ggccgcataa cattcgcatg gtggtggttt tgccggcgcc gttggcgccg agaaagccaa
     2461 agatggttcc tggcgcgatt gtaaatgaaa gatcttgcac ggcatggaca tcgccaaaac
     2521 tcttgctcag gtcgttgagc acgattaccg gtttgttcat ctcgaatcct tattgcgatg
     2581 atcccttaaa tttgcaacca tctgtcacag atcgtcacat accgtctctc atgaaatttg
     2641 aaaccatgga accatctcga cgcctcctca ccaacgcatc cgtcctgctt ttattcctgc
     2701 tcgggagctt gaccaccgca cgcggcgacg ccgtttccga gctcgcctcg ttttccattt
     2761 tcgacaaggt cgatctcgcg caactcgcaa agagcgacgt caagacggcg catgggccgc
     2821 cgatgagtaa cccgcgtttt ctcgccgtcc aaagctgcta cgtggcgccg cgtgcgccgg
     2881 agcagctaat cgcagcgttg cgccgatggg acccaacgag acatagcgaa ctaaaagtgt
     2941 ttctgcactc cgatctgcca gccagcccca cagccgcgaa ttttgaaaaa ttgaaaagtg
     3001 ctccaaataa cggaccggtg cgcactttcg tagccgaaac tcaaaagctc agttctgatc
     3061 ttcaaatcag ccgggatgaa gcaaagaagt tttcggccgg aggcgccggt ggaggcggcg
     3121 ccatgccagc gtcggtcgtt gcgttttgga cggatgtgtt ggtctcgcgc acgaaaagtt
     3181 ttgtgtccgg gggaacatca gggcagccgc cctatgatca ttccggcaag gccatccggg
     3241 tgagcgatga agtaaacggt ctgctgcggg agcaaggaaa aatccagcgt caattttccg
     3301 gattacttgg cgctactggc attggtcgcg gcgcgggatc gctcaggccg caactttact
     3361 gggagttact cgatgtggat gatcacggcg tcgtaaccct tggagcggat tacaaccgcg
     3421 ctggcggagg cgggagttat caagccgctg acgtgcttta ctacgcgagc ggcggctatt
     3481 atgtcgcgct caccttgtac cagatgtggc ccgtcacggt agatggaaaa gcttccacgc
     3541 tggtttggcg cggtgatatg atttccgcgc cgtccctggc ctcgttgcac ggcgtggaac
     3601 ggctcgggtc cgaatcagtc atgatgaaac agatttccaa agccgttacg ctgtttcgtc
     3661 gtgacacaag tggaagccgt taagcgagtg aaaagtttcg cgcgtcgctt ctttctccgg
     3721 tcgctttgtc tcgcctcatt cttttggctg ggggcctcca tcctcgctca ggaaagcaac
     3781 cccgaaagct ttggggactg gttcgcttcg ggtgaatatg tcgtggagga aacgtacgtc
     3841 ggcagcggcg atgttcgacg caaccctcaa aaaagcgttc gcgattttga tgaacacgac
     3901 tcgatcctgc atcttgtgtt tactccgcga atcaaagccg gcgtattgcg tcttggagca
     3961 gagtgggagc gcttcacatt tggtattcca gacggcgcgc cgctgccaga tacgttgcaa
     4021 tcggtcaacc tggttattgg attggacacg cagctttcgg attccatcct gatccgagtc
     4081 gaagcgctgc ccggtgtcta taacatgggg ttcgatgatt tgtcggacga tttcaacgct
     4141 ccctttgttg ccggcggcac ttacatcttc accccggatc tacagctcgt cctcggggtg
     4201 agcgttgata ttgagcgcaa gtatccggtt cttcccgcgg ccggtgttcg ctggaaaatg
     4261 gcacgccagt gggtgctcaa tgctgtgcta ccgactccgc gtctcgagtt cgagatgaac
     4321 agtggtgtaa ccctttatgt gggagcgaat ttgaagcaaa acaattaccg cgtggaagat
     4381 cacttcggcg acaacagtgg ggtgccgcgg ctcaatcacg ctgtgattac ttataccgaa
     4441 gtgcgaaccg gcgcggggtt cgattggaaa atttctcccg gcgtgacgtt gagcggcgag
     4501 gtcggatacc agccgtacag agagttcgat ttttatcgtg cccacgtgcg ctactcccag
     4561 gacggcggcg ctccttacgg aatggtgtcg ctccaaggcg ccttctgacg aacctgtagc
     4621 cgggggcggt gaccccggtt cgcccgcgcg aagttctcgc ctcgtaagat cgcagttctt
     4681 cagctcaagg ttctgtcgag cgccccaatc ttgcgggtct gcggccagtg ccatgccacg
     4741 ccaagcacaa ccagaattgt gccgatgccg cccgcgacca ccgaaatcac cggcccaaac
     4801 agggcggcag tgagtcccga ttcaagtgcc ccgaattcgt tcgacgtgcc gataaaaata
     4861 ttgttcaccg ccgaaacgcg gccgcgcatt tcatccggtg tcatcagctg cacaatcgaa
     4921 cctcggatga tcacgctcac gctgtcgaac gctccaataa aaaacagcag ggcaagcgat
     4981 aaccagaagg ctttcgacaa gccgaaaagg atcgtcgcaa taccaaaacc agtcacacac
     5041 cagagcaaag ttttgccagc gcgtttcatc ggcggcaggt gcgctaccag taaagccatc
     5101 gcgaaagcgc cgacggccgg cgcggcgcgc atccagccca ggccgatggg tccacagtgc
     5161 agaatttgat cagcaaaaat gggcaataac gcggtggcgc cccccagcag caccgcgaat
     5221 aaatccaggg tgattgttgc caggatcacc tttttgctga acacgaatcg catgccggca
     5281 accagacttc tccacgtacc tttctcagtc tgatttcgcg attgggtgct ccggcggatc
     5341 ggcagaataa gaaggaaaaa gaaaaatgca caaagtgcat ccagcatata gacgaaaggg
     5401 aagccgacgt gcgccaccaa caatccgctg acgcctgggc cgacaaccga cccaatttga
     5461 aaaacgctgt tgttccacgt gaccgcgttc gcgaatgcgt cgcgtgacac cagcgtcgga
     5521 aaaaatgagc tgcgcgccgc ccaactgaat gtgcgcgcta ttgctccgat aaaaacaagc
     5581 agataaatca gcggaatgga agcgtcatcg aaatgaaacg tcggatgatg ccgctcgaaa
     5641 atcgcggcga tcgctgccag cgcgtgatta ccttcgcgca agagggcaat cggggggatg
     5701 gaaaggtgtt tccacgagac cagggcgagc gccatcgatg cgagtgcgct aaaaatctgc
     5761 gacacaagga taatgtgttt tcgactaaat cggtccgcca aatggccggc aggcaacgac
     5821 aacgcaacca ccggcaccgc aatcaccaat ccaaccaggc ccagtgcggt cgcagaatgg
     5881 gtgcgcgcgt agatttccca ttccaccgcc acggcgagca tgagacgacc ggtgattgag
     5941 aggaaattac cggctgtgaa cagagcgaat ccaccgaagc ggaaagctga ataaggatcg
     6001 tgcgcccggc gctctaactc aattggcggg gtaaccgccg ccggccgctg accctcgggc
     6061 gattcatagg accttggatc ggttggtggc ggcatgctcg agcgcgtccg ttcgaacacg
     6121 cgcacgagat tgtagattga gtggtgagcg tggcaaccca cgagaaggcg gagataatgg
     6181 cacgcttgtc ggtcccgaac cggcaatgcc gcccggccca gaaaaagtgc tcaaaaatct
     6241 gcggattttt cttccattcc ccgaaacggc tgcgctaaag tcggcggcct caggcaaaat
     6301 ggataacatc atcgaggcga aagaactcca gattgagcgc aagcatttct atgtggagct
     6361 gcgcgagaac gatcgcggga aattcctgcg catcaccgag gaagctcacg gccgtcgcaa
     6421 tagcatcatc gttcccagca ctggagtcga tgattttacg gctgcaattt ctgaagttct
     6481 aaccaacaac ggaagcgcgc cacaataaat cgacgggcaa actagtccgg cgatttttgg
     6541 cgagtcgctt tgttttcttt tgttcgcttg ccgcgttgac caccgtgacg cccgaaacta
     6601 ctttgcggtc cgccagctgc tggatcccct taacgctcct ctgcgcttct caatcatgac
     6661 cacaaaacga agatcgagca ttcaaaaaag atttcggcgc gcaagaacac gatcgacatc
     6721 gaaggtgcag cccaacgctg ccggaaagag aacgaaaacg ccgcgttatg tttattattt
     6781 tggcgacggt cacgccgacg gatcgggcag catgaaaccg ctcctggggg gcaagggcgc
     6841 aaacctccat gagatgaccc gcattggttt gcctgtgccg ccggggttca cgatcacaac
     6901 cgaagtttgc agttattttt atgatcacaa ccgcgcttac ccgccccaat tggaagctca
     6961 ggtagcggat gcgctcgcca gggttgagaa agccgtcgga aaaaaattag gcgacggaga
     7021 gcgacctttg ctcgtctcgg tacggtcggg tgcgcgggac tccatgcctg gaatgatgga
     7081 tacaatcctg aacctcggga tgaacgacaa ggtagtggag atcgttgcaa aaaaaacaaa
     7141 taacgcacgg ttcgcctggg attcctaccg gcgctttttg caaatgtatg gcgatgtcgt
     7201 catgggcgtg cagaaacgcg cgggcgaaga tcatgaaccc ttcgagatgg tgatcgagcg
     7261 tctaaaagat gaacgttacg gcaagcatga tttcccggac gtcagattga cggtcgccga
     7321 tttgaaggaa ctcgtgcagc gcttcaaggc gttgatcaaa gaacggaccg gaaaatcgtt
     7381 tccacaagat ccaaccgaac aactttgggg cgcgatcggc gcggttttcg gctcgtggat
     7441 gaacgatcgc gccatcgttt atcgccggaa gtacgggatt ccgcacgagt ggggtacggc
     7501 cgtcaacgtg cagacaatgg tgtttgggaa catgggaaaa acgagcgcga ccggagttgc
     7561 gttcacgcgc gatcccgcca ccggcgaaaa agtcttctac ggagagtatt tgatcaatgc
     7621 gcagggcgaa gacgtcgtcg cgggcgtgcg cacaccccat ccgattgccg acctggccaa
     7681 ggaaatgccg gcagcccaca aggagctgat gaaagtgcgc gccatcctcg aacgtcactt
     7741 caaggacatg caggacctcg agtttacggt ggaagaaaat cgtctctaca tcttacagac
     7801 gcgcaatggc aaacgcaccg gccatgctgc ggtccggatc gccgtcgata tggtcggtga
     7861 aaaattgatt cctaaaaaag aagcggtgcg acgtatcccg gccgattcgc tcgctcatct
     7921 tttggcgccg atcttcgacc ggcaagcggc cagggaagcg aaaaaaatcg gcagcggctt
     7981 ggccgccgga cccggcgccg catgtggtca cgttgtcttc agcgcggaag aagccgtggt
     8041 gcgcgcggaa aaaggtgaga aagtcgtgct tgcccgcatc gaaacctcgc cggaagactt
     8101 gcgcggaatg atcgcggcgg agggaattct aacctcacgc gggggcgtct cttcgcacgc
     8161 agctctggtc gcccggcaaa tgggaaaagt gtgcgtctgc ggcgcgaccg ggatccacat
     8221 cgattaccaa aatcgcaccc tgagcgcgaa cggcacgacg ttgcaccgag gagactacat
     8281 ctcaattgat ggaaccgcgg gcgaagtttt tgccggcgaa gtcgcgactg cgccgtcgga
     8341 gattgtccag gtgctcgttg atcgatcgct cgacccaaag aagagcgtta cttaccagga
     8401 gtacgcgaaa ttgatgaagt ggacggacga attccgcacc cttggcgtcc gcacaaacgc
     8461 cgacaccccc gagcaagtgg ccaacgcagt cgccttcggt gccgaaggga tagggctctg
     8521 ccgcacggag cacatgttct tcgaaggcaa tcggatcgac gctatgcggg aaatgattct
     8581 tgccggcacc aaagatgagc gcgggcgtgc gctggccaaa ttacttccct atcaacgcga
     8641 cgatttcatt ggcatcttta cagccttaaa aggtttgcct gcaacgatcc ggtttctcga
     8701 tccgccgctg catgaatttc ttccgtcgga tcacgcgcaa caaagcgaac tggcgaacaa
     8761 actcggcgtg accgttgaac aaatcgcacg ccgcgtgcat gagttgcatg aatttaatcc
     8821 catgctcggc catcgcggtt gtcgcctcgg gatcgttttt cccgaaattt ccgaaatgca
     8881 ggcacgcgcg atttttgaag ccgccgcgga ggtgcaaggc agcggaatta aggtccgccc
     8941 cgaaatcatg attccgttgg ttggatttcc gcgcgagtta aagcttcaga tcgatatcgt
     9001 ccgtcgcgtc gccaccgagg tgacgaagga aaggcagacc aaattcagtt atctcgtggg
     9061 aacaatgatc gaaatcccgc gcgccgcgct cgtggccgac cagatcgccc gcgatgcaga
     9121 gtttttcagc ttcggcacaa atgatcttac acagacgacg ctgggaatga gccgggatga
     9181 ctccggcagt ttcctcccga cttacgccga actcgacatc gtcgatacaa atccgttcgc
     9241 atcgatcgat cagaaaggcg tcggacgcct gatggaaatc acctgcgatt tgggacgcaa
     9301 aacccggccg gatatcaagc ttggaatttg tggcgaacac ggcggcgagc cgaccagtgt
     9361 caaattctgt gaacggctcg gcttggatta tgtaagctgc agtccattcc ggattccgat
     9421 tgcgcgcctg gcagcggccc aggcagccat cgagagctaa gttcgtggca acttttcagg
     9481 acatcgaagc ggcgcgcaag cgcattgcgg atgcggttta ttgttcgcct tgtcggcgtt
     9541 ccatcccact cagcgaactc acggagatgg aaattttctg caagttcgac aacttgcagc
     9601 gtacgggaag ttttaaagag cgcggtgcgc gcaatgcgct cgcgcagctg tcgcccgatc
     9661 agcagaagcg tggtgtcatc gcggcctccg ctggcaatca cgctcaggcg ctcgcgtatc
     9721 agggcaagtt gcttggtatt cccgccaccg tcgtgatgcc gaagttcgcg ccgctggtga
     9781 aagtgagcaa ttgccagaaa ctcggagcga cgatcgtttt gcatggaaga gatttcagcg
     9841 aagcgaaaga gcacgcgctc gaggtcgcga aagagaaagg cctcgcttac atcgacggtt
     9901 atgatgatcc cgcaatcatt gccgggcagg gaacgatggg cctcgaaatc atcgagcaaa
     9961 ctcctgatct cgatgtcgtc gttgttcccg taggcggcgc cggtttgctt gccggcgttt
    10021 ctttggccat taaaactttg cggcccaacg cggcaatcgt cgctgttgag gccgagaacg
    10081 tggcgagctt ttcagccgct gtcgaagcgg ggaaaccaac gaggatcgct attcatccga
    10141 cgctggcaga cggactcgcc attccacagg tcggttcgaa tgcatttcaa atcgcaaaac
    10201 cacttgttga tctgaccgtc actgttaccg aagaacagat cgctttgggg atcctgcgtc
    10261 tggtcgaatt ggagaaaagc gtggtcgaag gagccgcggc tacgccgctc gccgcgtgta
    10321 tgtccggaaa attgaaagag ttcgccggca aacgagtggt tcttttactt tgcggtggca
    10381 acatcgatcc gaacgtgctc agccgcgtaa ttgaacgagg cttggttgcg gatggccgcc
    10441 tcggccgatt cactgccgtc attagcgacc gccctggcgg cctggccgat ttggcagcgc
    10501 aaatcgcttc gattggcgcg agcatcaagc aagtagtgca cgaccgcgcc tttgccagcc
    10561 cggatgtttc ggccgtcaat gttctctgca cggtcgagac gcgaaaccat caacatctgg
    10621 ctgagcttcg cgcactgtta aagagtcgcg gcgtcaaaac ctacgattca aataatcccc
    10681 tgtagcggcg gtctgtgacc gtcgcactaa ttctcccggc gctcgtagaa caccgctacg
    10741 agacttgaag cgcgattcgc cttccgtagt ctctgtgaat gaaattgcac gaaagaacga
    10801 ctgaattcga ttcattcgtg cgtcgccaca tcggtccaaa ccaggaagag atcgatgcga
    10861 tgttggccga agtcggtttc gaaaatctcg acgccttgat cgacgctgcc gttccgagaa
    10921 atatccgcct ggatcggcaa ctgaacctgc cggaagcgaa atcggagatt gacgcgctgg
    10981 ccgaactgcg ggcgatttcg aagaaaaaca aagtcgcgcg ctcattcatc ggcgccggtt
    11041 attacgactg catcacgccg ccagttatcc agcgaaacat cctcgaaaat cccggctggt
    11101 acaccgctta cacgccttac caggccgaaa tcgctcaagg ccggttggaa gcacttctca
    11161 atttccaaac aatgatcaca gacctgaccg ggttcgacat tgctaacgcg tcgttgctgg
    11221 atgaagccac cgcagccgcc gaagcgatgg cgctttgtca cgcggtcgtt ccggatcgaa
    11281 aaactttttt tgtcgccgac aattgtcatc cgcagaccat tgctgtcatc caaacgcgcg
    11341 cgaagccgct cggtatcgag atcaagatcg acaattactc gcgtttcaaa ttcgacgaca
    11401 cggtttttgg cgcgctcgtc cagtatcccg ctaccgacgg cgcgatttac gattatagcg
    11461 atcttattaa ggaagcgcac aacaccggcg ctcttgttgt cgtcgcggcc gatattcttg
    11521 cgctgacgtt attgaaacct cccggagaat ttggcgccga tgttgccgtc ggtaataccc
    11581 aacgcttcgg cgttccgctg gggtttggtg gaccgcacgc ggcctacttc gccacacgcg
    11641 accgatacaa acgccatatg cccgggcgcc tggtcggcgt ttcgcacgat gccgatggcc
    11701 gtcctgctta tcggttggcc ctgcaaacgc gcgagcaaca tatccgtcgt gataaagcca
    11761 cgagcaatat ttgtacggct caggttcttc tggccgtgat cgcatcgatg tacgcggttt
    11821 atcacggtcc gcgcggtctg cgcgcaatcg cggaatgcgt gcatcgccag accagccaat
    11881 tggccgatgg cctgcgcgcg cttggtctga agatcgcaca cgaaaacttc ttcgacacaa
    11941 tccgcgtcga agttgaatcc agcgacgtca ttttggagca cgccgaaaaa gctggctgca
    12001 atttacgcgc gcttggctcg cgcgccgtct gcatttcatt cgacgaaatg acaacggttc
    12061 gcgatatcga gttgttgatg tcgatcttcc gcgggacgca tgttcgtgat tttgcggatg
    12121 ataatctcgg cgagccgccc atccgcatcc cgcaatccgc aatccgcact tcggattttc
    12181 tgactcatcc gatcttcaac acccacgaca ccgagacgga aatgctgcgt tatctaaaac
    12241 gactcgaatc gcgcgatctt tcgctgacca cttcgatgat tccgctcggt tcgtgcacga
    12301 tgaaattgaa tgccaccgcg gaaatgtttc cgatttcgtg gccggaaatt tccaggctgc
    12361 accccttcgc gcctgacgag cag
//
LOCUS       DASQUQ010000035         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_819308, whole genome shotgun sequence.
ACCESSION   DASQUQ010000035 DASQUQ010000000
VERSION     DASQUQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819308"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /gene="aat"
                     /locus_tag="VE486_01005"
     CDS             complement(<1..554)
                     /gene="aat"
                     /locus_tag="VE486_01005"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HZA37696.1"
                     /translation="MIDPELLLQGYRLGVFPMATGGGTIEWFSPDPRAILPLEDFHVT
                     HALRRLLRKKVFEITLNNRFSEVIEACARREDTWINREIIESYTQMHELGYAHSVETW
                     ASGKLAGGLYGVAIGGAFFGESMFHRVTDASKIALVALVEHLRARKFALLDTQWLTPH
                     LQQFGGVEISRNHYLRLLRRAVELP"
     gene            795..992
                     /locus_tag="VE486_01010"
     CDS             795..992
                     /locus_tag="VE486_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37697.1"
                     /translation="MKRTSILLITACLITGGCADPMEQTSAQEVQGQFERGVTGQGTL
                     GPIERPADDPASKHSVPQTHP"
     gene            997..2589
                     /gene="guaA"
                     /locus_tag="VE486_01015"
     CDS             997..2589
                     /gene="guaA"
                     /locus_tag="VE486_01015"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZA37698.1"
                     /translation="MKTVAPSAHSKILILDFGSQYTQVIARRIRECQVYSEIVPFDRP
                     AAEIAKMKPNGLILSGGPASVYDKGAPQIDKKIFSLGIPVLGICYGLMQMAHHLGGRV
                     VFTGRREYGAGMLQITNGSQLFDGLGNQLDVWNSHGDEVTALPKGFLTAGRTESSNFA
                     AVEDRQRKLYGLQFHPEVAHTPRGKEILQNFVYHICHCAMDWTMGSFIEEACARIRKQ
                     VGDQKVVLGLSGGVDSSVTAALLHKAIGDQLTCIFVNNGLLRSREEEIVQRVFGENFH
                     VRLKYVDASERFLGLLKGVTDPETKRKLIGNEFIQVFQHATEELLEEDQKNGGGHGGY
                     KFLAQGTLYPDVIESVSIEGNPAQVIKSHHNVGGLPEKMHFELVEPVRQLFKDEVRQA
                     GLQLGLPKEIVYRQPFPGPGLAVRILGEVTPERLSILREADTIVVSEMESSDWYYRVW
                     QSFAVLLPVRSVGVMGDQRTYENTIVLRIVESQDGMTADWVRLPYELLARISTRISNE
                     VKGVNRVCYDISSKPPSTIEWE"
     gene            complement(2606..>2816)
                     /locus_tag="VE486_01020"
     CDS             complement(2606..>2816)
                     /locus_tag="VE486_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37699.1"
                     /translation="AFFRGRSVARFNNEISSIQWDEIVFANQSQLHRVVLPEAASDAR
                     LSALNHAARNGKDFSEFIRAIGEIA"
BASE COUNT          672 a          755 c          789 g          600 t
ORIGIN      
        1 ggcagctcga cagcccggcg caagagacgc aggtaatgat tgcgcgaaat ctcgacgccg
       61 ccgaactgtt gcaggtgtgg cgtgagccat tgggtgtcga gcaatgcgaa tttccgggcg
      121 cgcaaatgtt cgaccagcgc caccaacgcg atctttgatg catcggtgac gcgatgaaac
      181 atcgattcgc cgaaaaacgc gcccccaatc gcgacaccgt aaagtccacc tgcgagtttc
      241 ccgctcgccc atgtttccac cgaatgcgca taaccgagct catgcatttg ggtgtagctc
      301 tcaatgatct cccgattgat ccaggtatct tcgcgtctgg cgcacgcttc gatcacttcc
      361 gagaaacgat tgttaagcgt aatctcaaaa actttcttgc gcaggagacg ccgcagcgcg
      421 tgcgttacat ggaaatcttc caggggcagg attgcgcgtg gatcgggcga gaaccattcg
      481 atcgtgccgc cccccgtcgc catcggaaaa acacccagcc gataaccttg gagcaaaagt
      541 tcgggatcga tcatggccgg tcaagcgtta aagcgttgaa gagttaaagc ggtaacctca
      601 ttgtcatgtt gagcgaagcg aagaatctct gattgattct gccagcagcg ttaaggtgat
      661 aatcagagat gtttcgcttc actcaacatg acagcatagt ggctggcggt tttaacgctt
      721 caactcggaa cattttcgcg aggaggcggc ttcaacctcc ttgcaatcaa ctggtcgctt
      781 tctaaaataa gcagatgaaa cgcacgtcga tcctgctcat cacggcatgt ctgatcacgg
      841 gcggctgtgc tgatccgatg gaacaaacca gcgcgcagga agtgcaaggt caatttgagc
      901 gcggcgtaac cgggcagggg accctcggcc caatcgagcg gcctgccgat gacccggcaa
      961 gcaagcatag cgtgccccaa actcatccgt agcgcagtga agacggtggc tccatcagcg
     1021 cactcgaaaa ttcttatcct ggatttcggc tcgcagtaca cgcaggtcat cgcgcggcgc
     1081 attcgcgaat gccaggttta ttccgaaatt gttccgttcg ataggccggc ggccgaaatc
     1141 gcaaagatga aaccgaatgg ccttatcctt tccggagggc cggcaagcgt ttacgacaaa
     1201 ggcgcgccgc agatcgacaa gaaaatcttt tcgttaggca ttcccgtgct cgggatttgt
     1261 tatggcctga tgcaaatggc gcaccatctc ggcggccggg ttgtcttcac cggccggcgc
     1321 gaatacggcg ccggcatgtt gcaaatcacc aacggctcgc agttgttcga tggacttggg
     1381 aatcaactag acgtctggaa tagtcacggc gacgaagtga ccgccctgcc aaaaggtttt
     1441 ctcaccgcgg gccggaccga aagctcaaac tttgccgcgg tcgaagatcg acaacgaaaa
     1501 ctttacggac tgcaatttca tcccgaagtc gcgcacaccc cgcgcgggaa ggagattctc
     1561 caaaatttcg tttatcacat ctgccactgc gcgatggatt ggacgatggg gtccttcatc
     1621 gaagaagctt gcgcgcgcat caggaaacag gtcggcgatc aaaaagtcgt gctcggtctg
     1681 agcggcggcg ttgattcttc agtgacggca gcgcttttgc acaaggcaat cggcgaccag
     1741 ctcacttgta tttttgtcaa taacggtctc ttgcgctcgc gcgaagaaga aatcgtgcag
     1801 cgcgtttttg gtgaaaattt ccatgtgcgt ctgaaatacg tggacgccag cgaacgcttt
     1861 ctcgggctcc tcaaaggcgt gaccgatccc gaaaccaagc gaaagctgat cgggaatgaa
     1921 tttattcagg tgttccaaca cgcgaccgag gagttgctcg aggaagacca aaaaaacggt
     1981 ggcggacatg gcggctacaa gtttctcgca caaggcacac tttatccgga cgtgatcgag
     2041 agcgtgtcga tcgagggcaa tccggcgcag gtgatcaaga gtcatcacaa cgtcggcggg
     2101 ttgcctgaga agatgcactt cgagctggtc gaaccggtgc gccaattatt caaagacgaa
     2161 gtgcgccagg caggattgca gctcggttta ccgaaagaaa tagtttatcg tcagcctttt
     2221 ccgggaccag gtctcgccgt tcgcattctc ggcgaagtca caccggagcg attgtccatc
     2281 ttgcgcgaag cggacacaat tgtggtgagc gagatggaat cttccgattg gtattaccgc
     2341 gtctggcaat cgttcgctgt cctgctgccg gttcgctcgg ttggcgtgat gggcgatcaa
     2401 cggacttatg aaaacacgat cgtgcttcgc attgtggaaa gccaggatgg catgaccgcg
     2461 gattgggtgc gattgcccta cgaattgctg gcgcgtattt cgacccgtat cagcaacgaa
     2521 gtcaaaggcg tcaaccgcgt ctgctacgac atctcgagca aaccaccgag cacgatcgag
     2581 tgggaataat tcgttagatc gacaatcagg cgatttcgcc aatcgcgcga atgaactcgc
     2641 tgaagtcctt gccattacgc gcagcgtgat tcagtgcgct gagcctggcg tccgatgcgg
     2701 cttcgggcag aacaacccgg tggagttgcg attgatttgc aaaaacaatt tcgtcccact
     2761 gaatcgatga aatttcgtta ttaaagcgcg caaccgaacg gccgcggaaa aacgcc
//
LOCUS       DASQUQ010000036         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_999695, whole genome shotgun sequence.
ACCESSION   DASQUQ010000036 DASQUQ010000000
VERSION     DASQUQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999695"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="glmS"
                     /locus_tag="VE486_01025"
     CDS             <1..1141
                     /gene="glmS"
                     /locus_tag="VE486_01025"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZA37700.1"
                     /translation="HRVSKVEFTAEDIKKGDFPHYMLKEIFEQPDTVRDAMRGRLSLE
                     DCTAKLGGLDMTPAQLRDVGRIVLTGCGTALHAGRVGEYLIERLANIPTEVEFASEFR
                     HRNTPMTPETLVFAISQSGETADTLGALRESRRKGFRTLGICNNVASTIARESDGGVY
                     MHAGPEIGVAATKSFTSQLVILTLIGLLFGRMRNLSTSEGSRIIEALEALPEQIEMVL
                     KLSDEIKTIAKKYAGVNGMLFFGRQFNYPIALEGALKMKEITYLFAEGHPSAELKHGI
                     IALVRPDLPSVFIVPDDAVFSKNVNNIEQVKARKGPIIALTSGNAAKQLKGIADEIIS
                     VPEALDCVMPILAVVPLQLLAYHLAVELGLDVDKPRNLAKSVTVE"
     gene            1290..2990
                     /locus_tag="VE486_01030"
     CDS             1290..2990
                     /locus_tag="VE486_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZA37701.1"
                     /translation="MPRSEDYVLGILQEGGLVTQPQIENARSRLNGAAGVVDVLINDG
                     IVSDSDISRTLAAQAQMDWIDISSIVIPPQVINQIRAEEARRFKVIPLAFGETGLVVA
                     VGDPLDIDTIDSLSFLLQRELELVCTSPKKIREALIKYYGTAEEAADVLQEKIGEDID
                     LGLEIGDGEAAAVDEAEAPIIRLVSMLLIEAHRAGASDIHLEPLDKKFRVRFRVDGVL
                     QEMQAPPKRLQSAIISRLKIMTGSMSIAEKRLPQDGRIQVKIKKKPIDLRVSTIPTNH
                     GESMVMRVLDKSSLKLGLPELGFFSDDQEIFERLIRLPDGILLVTGPTGSGKTTTLYA
                     CLNYVNKPDRKIITVEEPIEYQMTGINQVPVNPEIGMIFPAALRSILRQAPNIIMIGE
                     IRDLETASIATNASLTGHLVFSTLHTNDAPSAVARLVDIGVQPFLVASSVRAIMAQRL
                     VRRLCNNCKQPGELTETESRALRIESGQLQETQVMKPVGCEQCRQIGYKGRMGIFEIF
                     VIDDEVRHMINKRSSTLMLRQQARELGMRTLREDGVRKVLAGLTSAEEVISITIGDVS
                     "
BASE COUNT          715 a          850 c          893 g          666 t
ORIGIN      
        1 ccatcgagtc agcaaagtcg aattcacggc cgaggacatt aagaaaggcg atttccccca
       61 ctacatgctc aaggaaatct ttgagcaacc ggacacggtt cgggacgcga tgaggggccg
      121 tctcagcctc gaagattgca ccgccaagct gggcggcttg gacatgacgc cagcgcaatt
      181 gcgcgatgtt gggcgcattg ttctcaccgg ttgcggcact gctctgcacg ccggcagggt
      241 gggggaatac ttgatcgaac ggctcgcaaa tattcctacc gaagtcgaat ttgcgagcga
      301 gtttcgtcat cgcaatacgc cgatgacgcc cgagacactg gtgttcgcca ttagccagag
      361 cggcgaaacc gcggacacac tcggcgcgct gcgtgaaagc cggcgcaaag gttttcgcac
      421 cctggggatt tgcaacaacg tcgcgagcac gatcgcccgc gaaagcgacg ggggcgttta
      481 tatgcacgcc ggaccggaaa tcggcgtcgc ggccacgaaa tcttttactt cgcagcttgt
      541 cattctcacg ctgattggtc tgctcttcgg ccggatgcgg aatctttcca cctctgaagg
      601 aagccgcatc attgaggcgc tggaagcttt gcccgagcaa atcgagatgg tcctgaaact
      661 cagcgatgag atcaaaacca tcgcgaaaaa atatgccggt gtgaacggaa tgcttttctt
      721 tggccgccaa ttcaattatc cgatcgcgct cgaaggtgcc ctcaaaatga aggagatcac
      781 ttatcttttc gccgaaggcc atccgagcgc cgagctcaag cacggcatca tcgcgctggt
      841 gcgcccggat cttccctcgg tcttcatcgt gcccgacgat gcagtcttct cgaaaaacgt
      901 gaataacatc gagcaagtca aagctcgcaa gggcccaatc attgcactca ccagcgggaa
      961 tgccgcgaag caactcaagg gcatcgctga tgaaattatc tccgtgccgg aagcgctcga
     1021 ttgcgtaatg ccgatcctgg cggtggttcc acttcagctt cttgcctatc atcttgctgt
     1081 cgagctgggc cttgacgtgg ataaaccgcg taatctcgcc aagagcgtga cggtggagta
     1141 acacaggcag gttgcctgtt tggtcgagca ggcttccatc ctgcatcgcc acgggcgggc
     1201 aggatgcccg ctcgaggggg caaccaggat ggttgtgtta cgctgacaaa catttgcagt
     1261 aaattgtccg ttcaggcatc atttcactcg tgccacggag cgaagactat gtgcttggaa
     1321 tcctgcaaga ggggggactg gtcacgcagc cccagattga aaacgcacgc tcgcgtctga
     1381 atggcgcggc cggcgtcgtc gacgtcctga tcaacgatgg aatcgtttcc gactcggaca
     1441 tctcgcgtac gctcgccgcg caggcgcaga tggattggat cgatatttct tccattgtga
     1501 ttccgccgca ggtcatcaat caaatccgcg cggaagaagc gcgccggttc aaagtgatcc
     1561 cgctggcatt cggcgaaacc ggccttgtcg tggcagtagg cgacccgctc gacatcgata
     1621 cgatcgacag tctgagtttt ctccttcaac gcgagttgga actggtttgc actagcccta
     1681 agaaaattcg cgaagccctg atcaaatatt acggcacggc ggaggaagcc gccgatgtcc
     1741 tccaggaaaa aattggggaa gacatcgatc tgggcctgga aatcggcgat ggcgaggccg
     1801 cagcagtaga tgaagccgaa gcgccgatta ttcgcctggt gtccatgctg ctaatcgagg
     1861 cccaccgtgc gggcgcgagc gatattcatc tggagccgct tgataaaaaa ttccgcgtcc
     1921 ggtttcgcgt tgacggcgta ttgcaggaaa tgcaggcgcc tcccaagcgt ttgcaatcgg
     1981 ccattatcag ccggctcaag atcatgactg gctcgatgag catcgcggaa aagcgtctgc
     2041 cgcaagacgg ccgcatccag gtcaaaatca agaaaaagcc gatcgacctt cgcgtctcga
     2101 ctatcccaac caaccacggc gagagcatgg tcatgcgtgt gctggataag tccagcttga
     2161 agcttggtct gccggagctt ggattttttt ccgacgatca ggaaattttc gagcgtctga
     2221 tcaggctgcc ggatggcatt ctacttgtca ccggcccgac tggctccggc aagacgacca
     2281 cgctttatgc ctgtcttaat tacgtcaaca aaccagaccg caagatcatt accgtagaag
     2341 agccgatcga atatcagatg accgggatca atcaggtacc ggtgaacccg gagattggca
     2401 tgatctttcc ggccgcttta cgctctattt tgcgccaggc gccgaacatc atcatgattg
     2461 gcgagattcg cgacctcgaa actgcgagta ttgccacgaa tgccagcctg accgggcatc
     2521 ttgttttcag cactttgcac accaacgatg ctccttctgc cgtcgctcgt ttggttgata
     2581 tcggtgttca gccgttcctg gttgcgtcct ctgtgcgtgc gatcatggcg caaaggcttg
     2641 tccggcgtct gtgcaacaac tgcaaacaac cgggggaact gaccgagacg gaatcgcgcg
     2701 cactgcggat cgaatccggg caacttcagg aaacgcaggt aatgaaaccg gtgggctgcg
     2761 agcaatgccg tcagatcggt tacaagggac gaatgggcat atttgagatt tttgtgatcg
     2821 acgatgaggt gcgccatatg atcaataaac gcagctcgac tttgatgctg cggcagcagg
     2881 cacgggagct gggcatgcgg acgctgcgtg aagatggcgt gcgcaaggtt ctggccggcc
     2941 tgacctctgc tgaagaagtc atttccatta cgatagggga cgtgagctaa gccatttaac
     3001 gattcaacgg atccatgaat aaccaaaagc ttgccgagtt tttcgttgag cagcatgtct
     3061 tgcagccgtc gcaggtggaa gacgtgctga gcgaggccgc gttgaacgga aaatcgattg
     3121 agca
//
LOCUS       DASQUQ010000037         6508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_637521, whole genome shotgun sequence.
ACCESSION   DASQUQ010000037 DASQUQ010000000
VERSION     DASQUQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637521"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..1461
                     /gene="rlmN"
                     /locus_tag="VE486_01035"
     CDS             364..1461
                     /gene="rlmN"
                     /locus_tag="VE486_01035"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZA37702.1"
                     /translation="MAATDKIEVRSLLLNELQEELHNLGEPSYRAGQIADWLYQKRVK
                     SFDEMTDLSRDLRAQLANQFAFSKMDIVRVLGSHDTTRKFLFRLSDGNLIESVLIPAS
                     PASRDRGRGSSDRRTICLSTQVGCAYGCKFCASGLEGFSRNLRPNEIIDQIIAIEAAS
                     GEKIDNIVFMGMGEPLANFENVMRAIRMINASWGLGIGARHITVSTSGLAPQIKKLAD
                     EPLQIRLAISLHAATDEVRDQIMPINRKYNIEALLSACDYYVARKKQRLTFEYILIVG
                     VNDADDQAYLLARHARRLFAKVNLIPYNTVEGLEWTRPSRNRQEKFLSILRAHKIAAT
                     LRREKGHDIDAACGQLRLQTSRNERRQMITA"
     gene            complement(1468..3168)
                     /locus_tag="VE486_01040"
     CDS             complement(1468..3168)
                     /locus_tag="VE486_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZA37703.1"
                     /translation="MTQEDKDEQFYRDTESIAFTKLDDRQLALLEPLGKRRTLQRGEF
                     IFKAGQRDLGLTVILRGEIEVFEAREGTEQILATGRERDFSGDVAMLQGTSALASARV
                     KSEEAEILEVPAAEIRRALAELPGLGKPIVEALIMRRRRLMRDREFAGLRIIAKQDTR
                     EGHQLDDFLEKNRIPHRLIDFESEPGKALASRLHLTTRDLPTLITATGAPLRRPSLRE
                     VAQVAGLLRPLALEGEGEISCDLTIVGAGPAGLAAAVYAASEGLKTVVLESYAPGGQA
                     GSSSLIENFFGFPTGISGSDLTYQAQLQAYRFGAKFSTPAQALSFDYHDGEEYRAGLQ
                     VEGCSCILRAKCLLIATGADYRRLDADGREQFEGSGVYYAATAFEGRLCRGQTVIVAG
                     GGNSAGQAAMFLAEGAAKVLLVIRGTDISKTMSSYLSRRVQAHQNIEILFHTEIRKMT
                     GNKMLEAVELENTATHERREVKTPAVFSMIGAMPCTDWLPPEIERDEKGFIKTGPAVL
                     HAPAWKESKRPPGPLETSCPGIFAAGDVRSGSVKRCAAAVGEGGMAVAGVHEALGTFR
                     "
     gene            3331..4002
                     /locus_tag="VE486_01045"
     CDS             3331..4002
                     /locus_tag="VE486_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37704.1"
                     /translation="MAGYEGESEPMWDEYDWERFLQQQDQKTEQYMQLLETYLDDPQR
                     DEIIAREMGWTQLLDAKDWSAEVDALLDEDGDEEGDFTTDQAVRSADTFEEHSLYRAA
                     FALTIWIDQLFDQNASLQNESAAVKLATYSALASAKLAAALSNDHIDEIGMTIAYLKR
                     ALKAITISMDAAAQLRQPSKLITAAQHSVLQQRLFQVRDGIITLMGEYRSEWRRRFGS
                     EGFRS"
     gene            4030..4641
                     /locus_tag="VE486_01050"
     CDS             4030..4641
                     /locus_tag="VE486_01050"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZA37705.1"
                     /translation="MQTEATLAQADVSELDLIRRCQAGDTEAFDELVTRYRTRVFGMI
                     YNMIHSEQDAWDLAQDSFVKAWKSIKRFRGQSSFYTWIYRIVMNVTIDWARKKQIKAG
                     GAEFDDAIQLKEIDPASKTVPKADALPYETMERSEIRARIDRAIAQLSPEHRAVILMK
                     EIEEMQYHEIAETLGCSIGTVMSRLFYARKKLQNLLKDVYENI"
     gene            4628..5155
                     /locus_tag="VE486_01055"
     CDS             4628..5155
                     /locus_tag="VE486_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37706.1"
                     /translation="MKTFEEKWTAWLDGQLSDRELAEFEASLPDRAAAEAEKAEAKKL
                     GALLKRELGAQALLNEEFFNYQLRERIARENGGDSRQSGGRRIEVSTWWTIRRLLWTG
                     TASLAIFLAMAIFVMRDKNPAEQSQYLTQILNARVDPVVSPNATISMFETKEDRVTVL
                     WTEGLQSLPSDYAAK"
     gene            5162..5602
                     /locus_tag="VE486_01060"
     CDS             5162..5602
                     /locus_tag="VE486_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37707.1"
                     /translation="MARSLLLFVAFALVLSAAAELRGAETVWSGLVIAENVQQPQPIP
                     PELTRIEQPLKHLFGYNQFQVIGQSRKALKTGQEDWLATSKFFGLHVNAKGESEAGYV
                     LNLKLYKEKELLLETDAKLSRRSPLVIKGPQVGSGQLLLVLVVQ"
     gene            5603..5932
                     /locus_tag="VE486_01065"
     CDS             5603..5932
                     /locus_tag="VE486_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37708.1"
                     /translation="MVGRYVAGELPRLRFADGTFDLTLISYFLFVYQDRLSYEFHRES
                     ILEIMRVTRSEARIYPTVTSEAQPSEYVPMLRSDPALKHFEFAEIKTDFEFLVNSNSF
                     LRVTRRS"
     gene            complement(6098..>6508)
                     /locus_tag="VE486_01070"
     CDS             complement(6098..>6508)
                     /locus_tag="VE486_01070"
                     /inference="COORDINATES: protein motif:HMM:NF017317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HZA37709.1"
                     /translation="GAAEIATRTRGTPRTANNLLRWVRDYVQVKAEGKITAELADRAL
                     TMLEIDRDGFDQWDKRIIETLIHKFGGGPVGLNSLAVAIGEEAGTLEEVNEPYLIMEG
                     YIKRTPQGRVATANAYRKLGLKPPAGAQSELFGE"
BASE COUNT         1455 a         1814 c         1879 g         1360 t
ORIGIN      
        1 gagtgagcgg agtccgcgat ttcggcatcg gcgctgatac ttcccggcgt ccccgggcga
       61 tcaagcacaa tctcggttaa ttgggaaacg agatagttga acgggctacc agcgaacgct
      121 tgcgctggag ccaccggtcc cgggagcaaa gttgtcctca tccccggttc tttcacctgg
      181 gacgggggct ccactttgcg agatcggctt ctccgtcatc acttcgttgg gtgaggcaca
      241 ggcggagatc aagaaggcga ccgccaaaaa caacgtaacc cgaacggcaa cgcgctgaat
      301 catgggtgcg aacctaacca acggaaagtt tgatgcaagc tgtgccgtcc ggtaagcttt
      361 gcgatggccg ccaccgacaa gatcgaggtg agatcgctcc tgcttaacga gcttcaagag
      421 gagcttcaca atcttggcga gccttcttac cgcgccggac agatcgcgga ctggctttac
      481 caaaagcgag taaagtcgtt cgacgagatg accgatttgt cgcgcgattt gcgggcgcaa
      541 ctcgcaaacc aattcgcctt cagtaagatg gacatcgtgc gcgtgctcgg atcgcacgat
      601 accacccgca agttcctatt ccgtttgagc gatggaaact taatcgaatc tgttttgatc
      661 ccagcgtcgc ccgcgtcccg cgaccgcggg agaggcagtt cggatcggcg gacgatttgt
      721 ttatccaccc aagtcgggtg cgcttatggc tgcaaatttt gcgccagcgg cttggaagga
      781 ttttcccgca atctgcgccc gaacgaaatt atcgatcaaa tcatcgcgat cgaagccgca
      841 agtggtgaga aaatcgacaa catcgtattc atgggaatgg gtgaaccgct cgcgaatttc
      901 gagaacgtga tgcgcgcgat tcgaatgatt aatgcgtcat ggggcctggg catcggcgcg
      961 cgacacatta ccgtttcgac cagcggcctg gcgccccaaa tcaagaaact tgccgacgaa
     1021 ccattgcaaa ttcgtctcgc gatttcgttg cacgccgcga ccgatgaagt gcgggaccaa
     1081 atcatgccaa tcaatcgcaa gtacaacatc gaggcgttgc tttccgcttg cgactactat
     1141 gtggcgcgca aaaagcagcg gctcacgttt gaatacatct tgatcgtagg cgtaaacgac
     1201 gccgacgatc aggcgtatct tctcgcgcgg cacgccagaa ggcttttcgc aaaagtaaat
     1261 ttgattccgt acaacaccgt cgagggtctt gaatggacgc ggccttcgcg caatcgccag
     1321 gaaaaattcc tgtcgatctt acgcgctcat aaaattgccg cgacattgcg ccgggagaag
     1381 ggacacgaca tcgatgccgc gtgcgggcag ctgcgattac aaactagccg gaacgagcga
     1441 cggcagatga ttaccgctta gccattctca cctaaaagtg ccgagagctt cgtgcacgcc
     1501 ggcgactgcc atcccacctt caccgacagc agcggcgcaa cgcttcaccg atcccgagcg
     1561 cacatcgcct gccgcgaaaa tacccgggca gctcgtttca agcggaccgg gcggacgttt
     1621 gctctccttc catgccggcg catggaggac ggcaggtcca gttttgatga atcctttttc
     1681 gtcgcgttcg atctcgggcg gcagccaatc tgtgcaaggc atggcgccaa tcatggaaaa
     1741 gaccgctggt gttttcacct cacggcgctc gtgcgtagct gtattctcca gctcgaccgc
     1801 ttcgagcatt ttgttcccgg tcatttttcg aatctcggta tggaacagga tctcgatgtt
     1861 ttggtgcgcc tgcacgcgcc gggagagata gctcgacatg gtttttgaaa tgtcggtgcc
     1921 gcggatgacg agcaatactt tggccgcgcc ttccgcaaga aacattgcgg cctgtccagc
     1981 cgaattgccg ccgccggcga cgatgactgt ttgtccgcgg cagagccggc cttcaaatgc
     2041 cgtcgcggcg taataaacgc ccgagccctc gaactgctcg cgaccgtctg cgtccaatcg
     2101 ccggtagtct gcaccggtcg caatgaggag acatttcgcg cgcaggatac aagagcaacc
     2161 ctcaacctga aggccggcac gatattcctc tccgtcgtga taatcgaacg aaagcgcctg
     2221 agccggcgtg gaaaattttg caccgaaccg gtaggcctga agctgcgctt gataggtcag
     2281 atcgctgccg ctgattccgg tcgggaaacc gaaaaagttt tcgatcaggg aagaagaacc
     2341 ggcctgtccg ccaggagcat agctttccag gacaacggtt ttcaatccct cggacgccgc
     2401 atagaccgct gcggctagac ccgccgggcc ggcgccaaca atggtcaagt cgcagctaat
     2461 ctcgccttcg ccctcgagag ccagcggtcg aagcaaaccg gcgacctgtg caacttcgcg
     2521 caaggagggc cggcgtagtg gggcgccggt cgcagtgata agcgtgggca gatcgcgagt
     2581 cgttaggtgc agccgactgg caagcgcctt gcccggctcg ctttcgaagt cgatgaggcg
     2641 atgcgggatg cgattcttct cgaggaaatc atccagctga tgtccctcgc gcgtgtcctg
     2701 cttcgcgatg atgcgcagtc cagcgaactc acggtcacgc atcagccgcc gccgccgcat
     2761 aatcaaagcc tcaacaatgg gcttgcccaa accgggtaat tccgcgagcg cgcgccggat
     2821 ctcggctgcc gggacttcca gaatctcggc ctcttcagac ttcacgcgcg cactggcgag
     2881 ggcggaggtg ccttgcaaca tcgcaacatc gccgctgaag tcgcgttccc tgcccgtggc
     2941 gagaatttgc tcggtgcctt cccgcgcttc gaagacttcg atttcgccgc gcaggataac
     3001 ggtcagcccg agatcgcgtt gccctgcttt gaagataaac tctccgcgct gcaacgtgcg
     3061 ccgcttcccc aaaggctcga gcaacgccag ctggcgatcg tcaagtttcg taaatgcgat
     3121 gctttccgta tcgcggtaaa actgctcgtc cttatcttct tgagtcatga gatcgattgc
     3181 gcggagaaat cctggaacgg tcgggtcgtc acagtccagc ttacgcgaga atctacgact
     3241 tggtcaaaac gaccacgacg gcgcgcaaga caaatccgtg gtcgatgcaa aatcttttgc
     3301 gcagaaacgg aagctgacgt ttagtcgagc atggccggat acgagggcga gtccgaaccg
     3361 atgtgggacg agtacgactg ggagcgcttt ctccagcaac aggatcagaa gaccgagcaa
     3421 tacatgcaat tgctggaaac ctaccttgat gatccgcagc gtgatgaaat catcgcccgt
     3481 gaaatgggct ggacgcagtt gctggacgca aaggattgga gcgcggaggt cgacgcgctc
     3541 ctcgacgaag acggggacga agagggcgat ttcactacag accaggcagt gaggtcagcc
     3601 gacacttttg aggagcatag tctgtatcgc gccgcttttg ccctgacgat ttggatcgat
     3661 caactgttcg atcaaaacgc ttcgctccaa aatgaatcgg cggcggtgaa gctggctacg
     3721 tactccgcgc tggccagcgc caagctggct gcggccttga gcaacgacca tatcgacgaa
     3781 atcgggatga cgatcgcgta cctcaagcgg gccttgaagg cgatcaccat ttcaatggac
     3841 gcagcggcgc aactcagaca gccttcgaag ctgattactg cggctcagca ttcggtgctt
     3901 cagcagcggc tctttcaggt gcgcgatggc attatcactc tcatgggtga atatcgcagc
     3961 gaatggcgtc gccgtttcgg ctccgaaggc tttcggagtt gaatgaaata aggtcgattc
     4021 cgtccaatta tgcaaacgga agcgactctg gctcaagccg atgtatcgga attggacctg
     4081 ataagacgat gccaggccgg tgacaccgaa gcattcgacg agttggtgac tcgctaccgg
     4141 acacgagtat tcggcatgat ttacaacatg atacatagcg agcaggatgc ctgggatctg
     4201 gctcaggaca gcttcgtgaa agcgtggaaa tcgatcaaac gcttccgcgg gcaatcctcg
     4261 ttctatacct ggatttaccg gatcgtcatg aacgtgacga tcgactgggc gcggaaaaag
     4321 cagatcaaag ccggaggcgc cgagttcgac gacgcgatcc aattaaagga aatcgatccg
     4381 gcctctaaaa cagtgcccaa agcggatgca ttgccctacg agacaatgga acgaagtgag
     4441 attcgagcgc ggatcgaccg ggcaatcgcg caactttcac ccgaacatcg cgccgtaatt
     4501 ttaatgaaag aaattgaaga gatgcaatat cacgaaatcg cagaaacgct tggttgctcg
     4561 attggcacag tgatgtcacg ccttttttac gcgcgcaaga aactgcaaaa tttgctgaag
     4621 gacgtttatg aaaacatttg aagaaaaatg gactgcatgg ctggacggcc agctcagcga
     4681 cagggagctg gctgagttcg aagcttcgct gccggacaga gctgccgccg aagcagaaaa
     4741 agcggaggcg aagaaactcg gcgcgctcct gaagcgcgag ctcggcgcac aggcgttgct
     4801 gaatgaagag tttttcaact atcagcttcg tgagagaatc gcgcgagaaa acggcggtga
     4861 ctctcggcag tccggcggcc gccggatcga ggtttctacg tggtggacga tccgtcgcct
     4921 gctgtggacg ggaactgcgt cgctcgccat ctttctggcg atggcgattt ttgtcatgcg
     4981 cgacaagaat ccggccgagc agtcgcaata tctcacccag attctaaacg cgcgcgttga
     5041 tccagtcgtg agtccaaatg cgaccatctc gatgtttgag acaaaggaag accgggtaac
     5101 ggtgctttgg actgagggat tgcagtcgtt gccgtcggat tacgcagcta aataacggct
     5161 catggcccgc tcgctcctgc tctttgtcgc ctttgcgtta gtcctgagcg cggcggcgga
     5221 gttgcgcggc gcagaaacgg tttggagcgg tctcgtgatc gcggagaacg tgcaacagcc
     5281 gcagccgatt ccaccagagc tgactcggat cgagcagcct ttgaaacacc ttttcggtta
     5341 caatcaattt caagtgatcg gccagtcgcg aaaggcgctc aaaaccggcc aggaggattg
     5401 gctcgccacc agtaaatttt tcggcctgca tgtcaatgcc aagggggaga gcgaagcggg
     5461 ctacgttttg aatctgaaac tttataagga aaaagagctg ttgctcgaaa ccgacgccaa
     5521 actgagtcgg cgaagtccac tggtgatcaa aggtccacaa gtaggcagtg gacagttgct
     5581 cttggtattg gttgttcagt aggtggtagg gcgttacgta gcgggagagc tgccgaggct
     5641 gcggttcgcc gacggtacgt ttgatctgac tctcatttcc tattttcttt tcgtttacca
     5701 ggaccggcta agctacgagt tccaccgtga atcgatccta gaaatcatgc gggtgacgcg
     5761 cagcgaagca cgcatttatc cgacagtgac ctccgaagcc cagccaagcg agtatgtccc
     5821 gatgctacga tccgatccag cgctcaaaca ctttgaattc gccgaaataa agaccgattt
     5881 tgagtttctg gtgaattcca actcgttcct gcgcgtgacg cggcggtctt gaatcaaatc
     5941 gtttacgagg tttggggagc gcatgcgccc tcgcatgctg ccgatggcgc ctcgccatcg
     6001 tgaacttttc gaatgaattg gtttctcacg tgagtcgcgc gctggcgttt cggcaggggc
     6061 gccgaagcgc gtgcgctccc cggaaattcc gcacgcatca ctctccaaac agctcgcttt
     6121 gcgcgcccgc gggcggcttg aggcccagct tgcgatatgc gttcgctgtc gcgacacggc
     6181 cttgcggggt gcgtttgatg tagccttcca ttattaaata cggctcgttc acttcttcga
     6241 gtgtgccggc ttcttcgcca atcgcgaccg cgagcgaatt caaaccgaca ggaccgccgc
     6301 cgaacttgtg aatcaacgtt tcgatgatgc gcttgtccca ttgatcgaag ccgtcgcgat
     6361 cgatttccag catggtgaga gcgcggtcgg cgagttcggc ggtgattttc ccttcggcct
     6421 tcacctgcac ataatcgcgc acccagcgca ggagattatt cgctgtgcgc ggcgtgccgc
     6481 gcgtgcgcgt ggcgatctcg gccgcgcc
//
LOCUS       DASQUQ010000038        13390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_912400, whole genome shotgun sequence.
ACCESSION   DASQUQ010000038 DASQUQ010000000
VERSION     DASQUQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 13390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13390
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912400"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VE486_01075"
     CDS             <1..507
                     /locus_tag="VE486_01075"
                     /inference="COORDINATES: protein motif:HMM:NF039584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37710.1"
                     /translation="STCTMLYGHIETPVHRADHLRQLRELQDETGGFTGFVPFAFEPQ
                     TTVLSHVKRVSAFEQLRNLAVSRIYLDNIDHLTAYWVSLGLPLAQVSLSYGVDDLHGT
                     ILEEKIFHMAGATTPQQQSVTALEHAIREAGREPIQRDSFYRHLAASAQNGEKPASAA
                     DAAELACA"
     gene            613..1407
                     /locus_tag="VE486_01080"
     CDS             613..1407
                     /locus_tag="VE486_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HZA37711.1"
                     /translation="MNSLRIGCVKYLNARPLIHGWPGPVTFDHPAALCAQLANGDLDV
                     ALVSSFEFLRNPIYRIVDDISLSSDGPVYSVAVAHAGEISDIEEIELDPASATSVNLL
                     RCLLAERGLSPRLVQNTGDMPVFQASQRARLLIGDQAIRFRQRYGEQFRFWDLGEQWN
                     KLVGLPFVYALWLIRPEVRDAKSLAERLRKLRDENLADIDKLIAEEKEFDHDFCRRYY
                     RENLRFGFGEKEKAGLREFHKRCIELRLLPQEHLDPGAWDLELPLR"
     gene            1410..2288
                     /locus_tag="VE486_01085"
     CDS             1410..2288
                     /locus_tag="VE486_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZA37712.1"
                     /translation="MKRSGELRIGISGWRYPGWRGKFYPKGLPQRRELEYASRTLNSV
                     EINGSFYSLQLPSSYQRWYSETPDDFVFAVKGARYITHMKKLRDVKAPLANFFASGVL
                     ALREKLGPILWQLPPNLSWNRERLEEFFELLPRDTRTAAKLAKKHDNKLKARAWMKID
                     VSRPLRYALEVRHKSFMTPDFFDLLREHNVAFVFADTARKFPYAEDLTGDLVYIRLHG
                     AKKLYVSGYNGKALDWWAKRIELWGTSKQPRDAKTVTHAKARAKHLDVFVYFDNDAKV
                     HAPFDAQRLAARLGVK"
     gene            2952..4649
                     /gene="recJ"
                     /locus_tag="VE486_01090"
     CDS             2952..4649
                     /gene="recJ"
                     /locus_tag="VE486_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZA37713.1"
                     /translation="MQSRWIIAAPEELNGGEISWDEICGSECIARLLMRKGFASADEV
                     ENFLRPRLKSLSDPFLLPNMEAAVTRILAALDRQERIAIFGDYDVDGVTSMALLAEML
                     RAYGGVPELFLPLRMEEGYGLSPESVERCIEQHQPQLLIAVDCGTSSVAEIADLWRRS
                     VDVIVLDHHEPKSALPKCVAIVNPKIDPDSPFHYLCSVGIVFKLCHALLKTRPVSFDL
                     KSKLDLVALGTVADIVPLQGENRTFVHRGAIEIARSTRPGLKRLIEVSGVRPPILPEH
                     IGFRLGPRLNASGRLSNAQRSLQLLLTQDRAEAASLADFLDGQNRERQDIEKKVFLAA
                     EEQIGHHFDLIRDAAIVVAAHGWHPGVLGIVASRIARKYHRPTILIGIEANGIGKASG
                     RSIEGLNLVEALQRCSETLQKFGGHEMAAGLTIKEKDVDRFSETFRLTARELLSDGDL
                     QPCLRLDHELAFSDLNGDLLRWHEMLQPFGNGNPAPLFFAREVEPVAPSRVVNDKHRI
                     LRLRQGNYRRRAVYFDSADVPLPPAPWDIAFRIRADEYEGETVLGMQIQAVRKAMAD"
     gene            4653..6794
                     /gene="recG"
                     /locus_tag="VE486_01095"
     CDS             4653..6794
                     /gene="recG"
                     /locus_tag="VE486_01095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZA37714.1"
                     /translation="MESTKRTKVRDGKGAVTGTRGACVPEIDVCQALENLDWIQRPRL
                     LALRRLGIETVEDLLTHFPRRHEDRHQFPHFPREESDVPICLCGEVVKTSVRRFGGWK
                     KIFEATLEESSPNALSEPLTCRWFNLHYVQKMIATGQRLVVFGKPRLRGKRICMDHPE
                     FEVIENDEEISIHFRRITPIYPATEGLSQRVLRSIIYRLLNEISPARAGWKILLPAEL
                     ASDETRDAIRAIHFPESWEARDAAREHLVLSEFFAMQVLIASRRSESSTRLSEAHCGS
                     GSLLDKFLEALPFQLTGAQSKVIAEIQRDLAARHPMNRLLQGDVGSGKTVVAIAAMLL
                     AIEAAYQAAFMAPTQILAEQHYAVLRRWLEPLGVRMALRTAARQEESFLPLVAGDQDP
                     NVLIGTHALLYENVSFSNLGLVVIDEQHKFGVAQRARLAAREPAPDVLVMTATPIPRT
                     LTMTVYGDLDVSIIDEMPRNRGKVITAVRDVSKLGEVLSFLRTQLEAGRQLYVVYPLI
                     DESEKLDVKAASAEYELWRERLHPYPCEILHGRIPAPEKQDTMERFRRGETKALISTT
                     VIEVGVDVPNATVMLVENAERFGLAQLHQLRGRIGRGEHKSYCILLTSDQSKETCAKL
                     AVLERTNNGFEVAEADWDLRGPGDLLGTAQSGLPVLKIGNLKTDAGLMRRARAAAISI
                     FEADPRLELPENQRFWRLIVEQQGRTFSNVS"
     gene            6794..8314
                     /locus_tag="VE486_01100"
     CDS             6794..8314
                     /locus_tag="VE486_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZA37715.1"
                     /translation="MKNLAKFLLFVLVISAGISLLYDYRIKHGGLNLPSARQPEKYTL
                     ASTPSVDSKQVASLEALNRERRELVNSVIPSVVAVKTSKKIGIRREYGMDPFEFFFRN
                     PRQFRSPRDEALVQNSLGSGVIVTKEGHIITNNHVVDHVDEIEVQLSDGRTTKARLVG
                     ADEQLDLAVLKIDVPGVQPLKLADSDTVQPGDSVLAIGNPFGLQETVTDGIISWKGQP
                     NSTDFRGELLQTNAAINPGNSGGPLINVQGEVVGINEQIVSSSGGSQGIGFAIPSNTV
                     RTALESLLKQGRIIRGYLGIQTRALQPGQSNADSEGVTVEEVMPGSPAAQAHLQPGDV
                     IRKFNGRDVKNIYALRSMLAQTELNKNVELEIARAGKPLKVTTQIKEQPIDYQTGGVS
                     PRRGQPQPQAPQPPSGQDTTTSTLASIHVDALTPEMARQLDLPNNVRGVVVTNVDPDS
                     GVAELQKGDVIEEINQQPVTSVSDYNKIAASLNPSQPQVLSICRGRMRSFLVLRPR"
     gene            complement(8418..9089)
                     /locus_tag="VE486_01105"
     CDS             complement(8418..9089)
                     /locus_tag="VE486_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HZA37716.1"
                     /translation="MNTYLDKLDNDIAEKHLLKHPFYLAWTRGELSKEALMDYARQYY
                     HHVAAFPTYLSAVHAKCDDQATRKQLLGNLIDEEAGSPNHPELWIQFAKGLGVSDVDV
                     RNTDKQPETTNLIETFRSVCRDGSTAEGLAALYAYESQIPSICESKIDGLKRHYGFTD
                     PKHYGYFSVHIDADREHSAAEREMLSTSIDNHNFESVTRSVNRVLNALWEMLSGVCRR
                     HAIAC"
     gene            complement(9504..12863)
                     /gene="treS"
                     /locus_tag="VE486_01110"
     CDS             complement(9504..12863)
                     /gene="treS"
                     /locus_tag="VE486_01110"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HZA37717.1"
                     /translation="MAANGGETTIDPLWYKDAVIYELHVKTFCDSDGDGMGDFRGLIE
                     KLDYLQELGTTAVWLLPFYPSPLRDDGYDIADYFDVNPNFGTLDDFRAFLDAAHERNL
                     RVITELVINHTSDQNPWFQKSRRAIATAGIGDLRRAGSTPGPAVGTRGHNDDLAYRDF
                     YVWSDTPEKYKDARIIFKDFETSNWAWDPVAKAYYWHRFYSHQPDLNFDNPAVHDAVE
                     KVCDFWLALGVDGLRLDAVPYLYEREGTICENLPETHDYLRKLRAHVEAKFPNRMLLA
                     EANQWPEDAAAYFGKGDESHMNFHFPLMPRMFMSLQMEDRFPIIDILEQTPSIPDTCQ
                     WAMFLRNHDELTLEMVTDEERDYMYRVYASDPHTRINLGIRRRLAPLLASSRRKIELL
                     NTLLLSMPGTPIIYYGDEIGMGDNFYLGDRNGCRTPMQWSPDRNAGFSRANPQQLYLP
                     VTIDPEYHYEAVNVENQQKNLSSLLWWMRRVIAMRKNFKAFSRGSLEFLYPDNAKVLA
                     FLRRYENQTILVVVNLSRFAQSAELDLSRFSGCVPMEVFSRNLFRPIKKSRYVITLGP
                     HAYYWFALQAPTEGRRILKKPLVPTIHARAELDTLLNDGQREQLENEILPNYIQARRW
                     FASKARTLRNMKVVEQLAISSDANAGRLWFLEVSYLDGPTETYALPVKIVSGDAARAV
                     SQITPQAIIARFAGTEETILFDAIFDGKFRSQLFEVIAQRHLMKARAGDLMGIAGKTL
                     ETDPPVISDKSQVLGGEQSNSSMLFDNKFFLKLYRKLEDGVNPDVEINRFLTERTDFP
                     NVPAFLGAIEYRRPKAEPTVVCLLQSAATNEGDVWTLTLDAIGRYYERVLGRKADLQN
                     EIAPPGALLDELIGGIYPDKAKQMGQRTGELHLALASSMEDRAFAPEPFNAMAQRSVY
                     QNMRASLRRAFTFLEKKLSDLRPAFRDEATEVLGAEEEILAREKRLLDRHTNAAKIRI
                     HGDYHLGQLLYTGKDFVILDFEGEPARPLSERKLKRSALRDVAGMMRSFQYAAYSALW
                     QPAMRSEDVPFLERWADLWYREVSSVFLQSYLKTAADAIFVPQNAEDLQIMLEAYLLD
                     KAVYEIGYELNNRPDWVVIPIRGIKHILRSS"
     gene            complement(12973..>13390)
                     /locus_tag="VE486_01115"
     CDS             complement(12973..>13390)
                     /locus_tag="VE486_01115"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZA37718.1"
                     /translation="ATTSPAPATGKKMSVSSMLKDNEKRWSAAIARHDTATIESMVAP
                     DFIGVSSKGKVVNRRAMLAEVKADKDTYTSTKAEKLDVHMYGTGVAVVVGTANEKGTG
                     KDGKAFDRTYRFTDTWMDRGGNWQCIASQVMWVGQR"
BASE COUNT         2951 a         3603 c         3817 g         3019 t
ORIGIN      
        1 agcacctgca ccatgctcta cggacacatc gaaacgccgg ttcatcgcgc cgatcatttg
       61 cgtcaactgc gggaattgca ggatgagacc ggcggattta ccggcttcgt tccttttgcc
      121 ttcgaaccgc agaccacagt actctcgcac gtaaaacgcg tctccgcgtt tgagcagctt
      181 cgcaatctcg ccgtgagccg catttatctg gataacatcg accatctaac ggcttactgg
      241 gtcagccttg gtttgccgtt ggcgcaggtc tcgctcagct acggtgtgga tgatctgcac
      301 ggcacaattt tggaagaaaa aatcttccac atggcggggg caactactcc gcaacaacaa
      361 agtgtgacgg cgctcgaaca cgccatccgc gaagccggcc gagagcccat ccagcgcgac
      421 agcttttacc gtcatttggc cgcttcggcg caaaacggag agaaacccgc atcggcagcg
      481 gatgcggcgg aacttgcctg cgcgtgattc caaaatgtga acagtgatcg gtgaacggtg
      541 aactgagcgt cccgttggtt acgcccaatc tatcttggtt gccttgatca ctgatcacgg
      601 ttcactggtc acttgaactc cctccgcatc ggctgcgtca aatatctcaa cgctcgtccg
      661 ctcattcacg gctggccggg tccggttacg ttcgatcatc ccgccgccct ttgcgcacag
      721 cttgcgaacg gcgatctcga cgttgcgctc gtctccagtt tcgaatttct gcgcaacccg
      781 atctaccgca tcgttgatga tatatccctc tcatccgacg gaccggttta cagcgtggca
      841 gtggcccacg ccggagagat atccgacatt gaagaaatcg aactcgatcc cgcatccgca
      901 acgtccgtca atctactgcg ttgcctgttg gccgaacgcg gattgagccc gcgcctggtc
      961 cagaacacag gcgacatgcc tgtgttccag gcatcacagc gggcgcgcct gctcatcggc
     1021 gatcaggcca tccgcttccg gcaacgatac ggggaacaat ttcgtttctg ggatctcggt
     1081 gaacaatgga acaagctggt tggtcttccg ttcgtgtacg cactctggtt gattcggcct
     1141 gaagttcgcg atgcgaagtc actcgcggaa cgcttgcgca agctacgcga tgaaaacctg
     1201 gccgacatcg acaagttgat tgctgaagaa aaagaattcg atcacgattt ttgccgtcgt
     1261 tactacaggg agaatttgcg attcggcttt ggagaaaagg aaaaggcagg tttacgcgag
     1321 tttcacaagc gctgcattga actgcggctt ctgccgcaag agcatttgga tcctggagcc
     1381 tgggatttag agcttccgct ccgttgatta tgaagcgcag cggggagttg cgcattggaa
     1441 tctctggatg gcgctacccc gggtggcggg ggaagtttta tccgaaaggc ctgccgcagc
     1501 ggcgcgaact tgaatacgcg agccgcacac tgaattcggt cgagatcaac ggatcatttt
     1561 attcgctgca actgccatcg agttatcagc gatggtattc agagacgcca gacgattttg
     1621 ttttcgcagt caaaggcgcg cgttacatca cgcacatgaa aaagctgcgc gatgttaagg
     1681 cgccacttgc aaactttttc gcttccggcg tgctcgctct tcgcgaaaaa cttgggccga
     1741 ttctctggca gctcccgccg aacctcagct ggaatagaga acgcttggaa gaatttttcg
     1801 aattattgcc gcgcgatact cgcaccgccg cgaagcttgc caaaaagcac gataacaaat
     1861 tgaaagcgcg cgcctggatg aagatcgatg tctctcgccc actgcgctac gcgctcgaag
     1921 ttcggcacaa aagcttcatg acgccggact ttttcgacct gttgcgcgaa cacaacgtcg
     1981 cctttgtctt tgccgatacc gcgaggaaat ttccttatgc tgaagatttg accggcgatc
     2041 tagtttacat ccggttgcac ggcgcgaaaa aactttacgt cagcggttac aacggcaagg
     2101 cgctcgactg gtgggctaag cgcatcgagt tgtgggggac aagcaaacag ccgcgcgatg
     2161 ccaaaacggt tacgcatgcc aaagctcggg ccaaacatct tgacgttttc gtttacttcg
     2221 acaacgacgc caaggtccac gctcccttcg acgcacagcg actggcagcg cggctgggag
     2281 tgaagtgaat aatgattcgc atgagttccg tcaaagcgct caactccgaa agaattccgg
     2341 gtcaacggga aggcaccgga ccaacctttg tccaatattg aagagtacaa tcgtctcccg
     2401 gaaaagacgt gaatttggtc ggccgcggac ggccaggccg gccccagcag gcacgtttat
     2461 gggtcgcatc gaggatctca tatatgccga gtgttagttg accggcgtgc ggtccgccaa
     2521 ctgcgattga gtccacgcgc ttgggtctcg gaataggatc taccgtaaag gttccggccg
     2581 gatgggttcc gacgccgctg gctttgggaa aacgaaaggt attgtgatcg gtgatcagga
     2641 ttgtatttcg caccacgctt tggggcacaa gccgaccatc aaccacggcg cgagtcaatt
     2701 gccaggttcc cgtgagcagc tcataatccc tctcggcggc ggcagaccgc gtcgttgagc
     2761 cgccgaattg tctatttatg cagccagcgc caatcgctgc gactgttcct attaacaaga
     2821 gcgatattac tttcatccta gctgttattt catccggaat ggccgctttg tcgagccaac
     2881 gtgcctccag cccggacctc aagcgagctt gctgcatctt gtcaggaagt gatccgcgaa
     2941 taagctaatc ggtgcaaagc cgatggatta ttgccgcccc tgaggagcta aacggcggtg
     3001 agatctcgtg ggatgaaatt tgcggatcgg aatgcatcgc gaggctgctg atgcgcaagg
     3061 gcttcgccag cgccgatgag gttgaaaatt ttctgcggcc gcgtctcaag tcgttaagcg
     3121 atccattttt gcttccgaat atggaagcag ccgtgacgcg cattctggct gcgttagatc
     3181 gacaggaacg catcgcaatt ttcggagatt acgatgtcga tggggtaacc tcaatggcgt
     3241 tgctcgcgga gatgttgcgc gcgtatggcg gcgtacccga attatttttg ccgttacgga
     3301 tggaggaagg ctacggcttg agtcccgaaa gcgtcgagcg ctgcattgag caacatcagc
     3361 cacaactcct gatcgcagtc gattgcggta catcatccgt ggccgagatc gccgatcttt
     3421 ggcggcggag cgtggatgtg atcgtcctcg accatcacga gccgaaatcg gcattgccca
     3481 aatgcgtggc gatcgtaaat ccgaaaatcg atcccgactc gccttttcat tatctgtgca
     3541 gcgtcggaat cgtctttaaa ctctgtcacg cattgttaaa aacgcgtccg gtcagtttcg
     3601 atctcaaatc gaagcttgac ctcgtcgcgc tcggcacggt ggctgatatc gtcccgttgc
     3661 aaggtgagaa ccggactttt gttcaccgcg gcgccattga aattgcaaga tcgacaagac
     3721 cgggcttgaa aaggttgatc gaagtgagcg gtgtgcggcc gccgattttg ccagagcaca
     3781 tcggctttcg ccttggtcct cgactcaatg catccgggcg gctgagcaat gcccagagat
     3841 cgctccaact tctgctaacg caggaccgag ccgaagcggc gtcgctggcc gattttctcg
     3901 atggccaaaa tcgcgagcgg caggatatcg aaaagaaagt gttcctcgcg gcggaggaac
     3961 aaatcggtca ccactttgat ctgatccgag acgcggcaat cgtggtcgcg gcacacgggt
     4021 ggcacccagg ggtgctggga attgtcgcct cgcgaattgc gcgcaagtat catcggccaa
     4081 ccatcctgat cgggattgag gcaaacggta ttggaaaggc aagtgggcgt agtatcgaag
     4141 gattaaatct tgttgaagca ttgcagcgtt gcagcgaaac gctgcagaaa tttggaggac
     4201 acgaaatggc ggctggtctt acgataaagg agaaagacgt cgatcgtttc tcggaaactt
     4261 ttcgtctaac ggcgcgcgaa cttttgtcgg atggagattt gcagccttgt ttgcgccttg
     4321 atcacgaact ggccttttct gatctcaatg gcgatcttct gcgatggcac gaaatgctcc
     4381 agccattcgg caacggaaac ccggcgcctc ttttttttgc gcgcgaagtg gagccggtgg
     4441 cgccgtcgcg ggtggtgaac gacaagcatc gcattctgcg cctgcgtcag ggcaattatc
     4501 gtcggcgcgc ggtttacttc gattcggccg acgtaccgct gcccccagcg ccgtgggaca
     4561 tcgctttccg gattcgggca gatgaatacg aaggcgaaac tgtgctgggc atgcagattc
     4621 aggcagttag gaaagcaatg gcagattgat taatggaaag tacaaaaagg acaaaagttc
     4681 gtgacggcaa gggcgccgtc accggcacgc gaggcgcgtg cgttcccgaa atcgacgtct
     4741 gccaagcgct ggagaacctt gattggatcc agcggccgcg tttactcgcg ctgcgccgcc
     4801 tcggcatcga gacggtggag gatttgctca cgcattttcc gcgtcgtcac gaagatcgac
     4861 atcagtttcc ccattttccg cgcgaggaga gcgacgttcc aatttgcctt tgcggcgagg
     4921 ttgttaaaac gtctgtgcgg agatttggcg gatggaagaa aatctttgaa gccacgctgg
     4981 aggagtcgag cccaaacgcg ctgagcgagc cgctcacatg ccgctggttc aacctgcatt
     5041 acgtgcaaaa gatgatcgcc acggggcagc gtctcgttgt ctttggcaaa ccgcgtttgc
     5101 gtgggaagcg gatttgcatg gatcaccccg agttcgaggt catcgagaat gacgaggaga
     5161 tctcaatcca ttttcggcgc atcaccccga tctatcctgc tacggaaggt ctttcacagc
     5221 gcgtgttgcg cagcatcatt taccggttgc tgaacgagat ctcccccgcg cgtgcggggt
     5281 ggaaaatctt gttgccagct gagctggcga gtgacgagac acgtgacgcg attcgcgcga
     5341 ttcattttcc ggaaagctgg gaagcgcgtg acgctgctcg cgagcatctg gtgctctccg
     5401 agttcttcgc gatgcaagtg ctgattgcat cacgacgctc cgagtcgtcg actcgcttga
     5461 gcgaagccca ctgcggctct ggctcgctgc tcgataaatt tttggaggcg ttaccattcc
     5521 agttgactgg tgcgcaatcg aaagtaattg cggaaatcca gcgcgatctg gcggcgcgtc
     5581 acccgatgaa ccgtttgctc caaggggacg tgggctcggg caaaacggtc gtcgccatcg
     5641 cggcgatgtt acttgcgatc gaagcggcgt atcaggccgc gttcatggcg ccgacgcaaa
     5701 ttctggctga gcaacattac gccgtgctcc ggcgctggct cgaaccgctt ggggtgcgca
     5761 tggctctccg gactgcagcg cgacaggagg agagcttcct cccattggtt gccggcgatc
     5821 aagatccgaa tgtactcatt gggacgcacg cgctgcttta cgaaaacgtt tcgttttcga
     5881 atctcggact cgtcgtgatc gatgagcagc ataagttcgg ggttgctcag cgcgctcgat
     5941 tggcagcgcg cgaaccagcg cccgatgtgc tggtgatgac ggccacgccg atcccaagga
     6001 cgctgacgat gacggtttac ggcgacctcg atgtttcaat tatcgatgag atgccgcgca
     6061 accgcggaaa ggtaattacc gccgtgcgcg acgtatcgaa gctcggcgaa gttctcagct
     6121 ttttgcggac gcaattggaa gcgggtcgac aactctatgt ggtctatccg ttgatcgatg
     6181 agagtgaaaa attggacgtg aaagctgcct cggcagagta cgagttgtgg cgtgaacggc
     6241 tgcatcccta tccctgtgaa atacttcatg gccgtattcc ggcgccggag aaacaggata
     6301 ccatggaacg atttcgtcgg ggcgagacca aagcattaat cagcaccacg gtaattgaag
     6361 ttggtgtcga tgttcccaac gcgacggtaa tgctggtcga aaacgcggag cgcttcgggc
     6421 tggcgcagct tcatcagctt cgcggacgga tcggccgggg ggagcacaaa tcgtattgca
     6481 ttttgttaac gtcggaccag tcgaaagaaa cgtgcgctaa gctcgctgtt ttagaacgaa
     6541 ccaataacgg ctttgaagtg gcggaagcgg attgggattt gcgcggcccg ggtgacttgc
     6601 tgggtacggc gcaaagcggt ctgcccgtgc tcaagattgg caacttaaag accgatgccg
     6661 gcttgatgcg acgcgcgcgt gcggcggcta tatcgatctt cgaggcggat ccacgcctgg
     6721 aactgcctga gaatcaacgt ttctggcgtt tgattgtcga acaacaggga cgaacttttt
     6781 ccaacgttag ctgatgaaaa acctcgccaa atttctgctt tttgtgctgg ttatcagtgc
     6841 aggaatttcc ctgctttacg attaccggat caaacacgga gggttgaacc tgccctcggc
     6901 tcggcagccg gaaaaataca cgctggcttc aacgccgagt gtggattcaa agcaggtcgc
     6961 atcgcttgaa gcattgaacc gggagcggcg cgagctcgtc aacagcgtga tcccatccgt
     7021 tgtcgcggtg aaaacatcga agaagatcgg catccggcgg gagtatggta tggatccttt
     7081 cgagtttttc tttcgcaatc cccggcagtt tcgcagccct cgcgacgagg cgctggtgca
     7141 gaattcgctc ggctctggcg tgattgtgac gaaggaaggc cacattatca caaacaatca
     7201 tgtggtcgat cacgtggacg aaattgaagt gcaattgagc gatggacgca cgacgaaagc
     7261 gcgtctggtg ggtgccgatg aacagctcga tctggctgtg ctcaagatcg atgttccggg
     7321 cgtccagcca ctgaagctgg cagactctga cacggtgcaa cctggagact ctgttctggc
     7381 cattggcaat ccgtttggtc tccaggagac ggtgacggac ggaatcatta gctggaaggg
     7441 gcagccgaac agcacggatt ttcgcggcga gctgctccaa acgaacgcgg cgatcaatcc
     7501 agggaacagc ggcgggccat tgattaacgt gcagggggag gtcgtcggca ttaacgaaca
     7561 aatcgtttcc agcagtgggg gttcgcaggg aatcggcttc gccattccat cgaacacggt
     7621 tcgcactgcg ctcgagagtt tattgaagca ggggcggatc attcgcggtt acctcgggat
     7681 ccagacccgc gcattgcagc cgggccaaag caacgcagac agcgaaggcg tgacggtcga
     7741 ggaagtaatg ccgggatcgc ccgccgctca ggcgcaccta caaccaggcg atgttatccg
     7801 caaattcaat ggccgcgacg tgaagaatat ctacgcgctg agaagtatgc tggcacagac
     7861 cgagttgaat aagaacgttg agctcgagat cgcgcgggcc ggcaagccgt taaaggtgac
     7921 cactcagatc aaggagcagc cgattgatta tcaaacgggc ggcgtttcgc ctcgacgtgg
     7981 ccagccgcag ccgcaggccc cgcagccgcc cagtggtcaa gacacaacca caagcactct
     8041 cgcgtcaatt catgttgacg cgctgactcc ggagatggcg cgtcagctcg atctgccaaa
     8101 caatgtgcgc ggcgtcgtgg tcaccaacgt cgatcctgat agtggcgtgg ccgaattaca
     8161 aaaaggtgac gtgattgagg aaattaatca gcaaccagtg acgtcggtct cggattacaa
     8221 caagatcgct gcatcgctta accccagcca accgcaggtt ttatcgattt gccgtggtcg
     8281 gatgcgttcg tttctcgtct tgcgcccgcg ctgaggcgct gccgatgtag ccgcctcgct
     8341 gtgtcgaggc gctgcgtgga gtggtgaccg gcacggcgac acagcgccgt ggctacacct
     8401 ccccgagcca acacgcctca gcacgcgatc gcgtgacggc gacaaacgcc ggaaagcatt
     8461 tcccaaagcg cattaagaac gcggttcacc gatcgtgtta cggactcgaa attgtgatta
     8521 tcgatggatg tgctaagcat ctcgcgttct gcggcggaat gttcgcggtc cgcatcgatg
     8581 tggacgctga agtatccgta atgtttcgga tcggtgaagc cataatgcct ttttaatccg
     8641 tcgatcttcg actcgcagat cgaaggaatt tggctctcgt atgcgtaaag agcggcgagg
     8701 ccttccgctg tcgatccatc gcgacagact gatcgaaaag tttcgatcaa gtttgtagtc
     8761 tccggttgtt tgtcggtgtt tcgcacatcg acatcggaaa ctccgaggcc tttcgcgaat
     8821 tggatccaaa gctcaggatg attcggcgag ccggcttctt catcgatcag gttacccagc
     8881 aattgcttcc gcgtcgcctg atcgtcacat tttgcgtgca cggcggagag ataagtcgga
     8941 aacgcggcga catggtgata atattgccgc gcataatcca tgagcgcctc tttgcttagt
     9001 tctccgcgcg tccacgcgag atagaacggg tgtttcaaga gatgtttctc tgcgatgtca
     9061 ttgtcgagct tgtcgagata tgtgttcatc aattttctcc gaatatggtt taagccacag
     9121 actgccacag attttcacag attaaagaca cagaattctt atctggatct catctctgtc
     9181 catctctggc tgcgaaaatt caggcggtac tccgattaat gacaccgttc atggcaattg
     9241 tccgcctgct tattgcatct gtttacgcag gggccacgat tgcaagcgtc cacgcagtgg
     9301 ctgcgattac attcgcactg ctgatgacaa tcctttacgt cggaacaatc gcgcggccaa
     9361 ttggctgaga acactgctgc gacgccaagg atgccacctg caagcaaggc agtcgcaacc
     9421 catgttctta tggtatttct catagtttcc cctttcttct taacggcacg tgtcaaataa
     9481 cgtctagtcg caattgcatt tctctaggag gatcgcaaaa tgtgtttgat accgcggatg
     9541 gggatgacga cccagtcggg gcggttgttt agttcgtagc cgatttcgta aacggctttg
     9601 tcgagcaaat aagcttcgag catgatttgc aaatcctcgg cgttttgtgg cacaaaaatc
     9661 gcgtcggcgg ctgtttttaa ataactttgc agaaagacgc tgctgacctc gcgataccaa
     9721 aggtcggccc agcgctcgag gaatggcacg tcctcggaac gcatggctgg ctgccagagc
     9781 gcgctgtagg cagcgtactg aaatgagcgc atcattccgg cgacatcacg cagggcggag
     9841 cgcttcagtt ttcgctcgct gagtggccgc gcgggctcgc cttcaaaatc aagaatcacg
     9901 aaatctttcc cggtataaag caactggccg agatgataat cgccgtggat ccgaattttc
     9961 gcggcattgg tgtgacggtc gaggagccgt ttttcgcgcg cgagtatctc ttcttccgcg
    10021 ccgaggactt ccgtagcttc atctcgaaat gccggacgca gatcagacaa tttcttttcg
    10081 agaaacgtaa acgcgcgccg aagcgatgcc cgcatgtttt ggtaaacaga gcgctgcgcc
    10141 atggcattga atggctcagg cgcaaaggcg cgatcttcca tgctggatgc gagcgcgagg
    10201 tgcagttcac cggtacgttg tcccatctgt tttgccttgt caggataaat gcccccgatt
    10261 agttcatcga ggagggcacc tggtggcgca atttcattct ggagatcggc tttccgtccc
    10321 agcacccttt cgtagtagcg gccaatggca tccagagtga gcgtccacac gtcgccttcg
    10381 tttgtggcag cgctttgcaa gagacaaaca acggtcggct cagcctttgg ccggcgatat
    10441 tcgattgccc cgaggaacgc tggcacattt ggaaaatcgg tccgttcagt gaggaaacgg
    10501 tttatttcca catcaggatt cacgccatcc tcgagtttgc gatacagctt aaggaagaat
    10561 ttgttatcaa agagcatcga agagttgctc tgctcaccgc ctaaaacctg cgacttgtca
    10621 gagatgacgg gaggatccgt ttcgagcgtc ttgccagcga tccccataag atcgcccgcc
    10681 ctcgccttca tcaggtgacg ctgcgcgatt acctcgaaca attgagagcg aaacttcccg
    10741 tcaaagatcg catcgaacag gatcgtttcc tcagtcccgg cgaaacgcgc gatgatcgct
    10801 tgtggcgtga tctgagaaac agcccgcgcg gcgtcaccgg acacaatctt taccggaagg
    10861 gcatacgttt ccgtcggtcc atcgagatag cttacttcaa ggaaccaaag tcgtcccgca
    10921 ttagcgtccg acgagatggc gagctgctcg acgaccttca tattgcgcaa ggtccgcgct
    10981 ttggaggcaa accagcggcg ggcctggatg taattgggta ggatttcgtt ctcgagttgt
    11041 tcgcgctggc catcgttaag caaggtatcc aactcggccc gcgcatggat cgtcggcacc
    11101 aggggcttct tcaggatccg gcgcccttcg gtcggcgctt gcaaggcgaa ccagtagtag
    11161 gcatgtgggc ctagcgtgat tacgtagcgc gatttcttga ttgggcgaaa cagattgcgg
    11221 ctgaagactt ccatcgggac acagccgctg aagcgcgaaa gatcgagctc agccgactgc
    11281 gcgaaacgcg agaggttcac taccaccaga attgtttgat tctcgtagcg gcggaggaag
    11341 gccagaactt tcgcattatc cggatagaga aattccaggg agccgcgcga gaacgcctta
    11401 aagtttttcc gcatcgcaat gacgcggcgc atccaccaca gcagtgaaga gagatttttc
    11461 tgctgattct caacgttgac cgcttcgtag tgatattccg gatcgatcgt aaccggcaga
    11521 taaagctgct gcggattagc tcttgaaaat ccggcattgc ggtccggact ccactgcatg
    11581 ggggtgcggc aaccgttgcg atcgccaaga taaaagttgt ctcccatgcc gatttcatct
    11641 ccgtaataaa tgatcggcgt gcctggcatg gagagcagga gcgtgttaag caattcgatt
    11701 ttgcggcggc tgcttgcgag cagtggcgcg agccggcggc ggataccaag attaatgcgc
    11761 gtgtgaggat cactggcgta aacgcgatac atgtaatcgc gctcctcgtc agtcaccatt
    11821 tccagcgtta gctcatcatg gttccgcagg aacatcgccc actggcaggt gtcgggaatc
    11881 gacggagttt gctcgaggat atcgatgatc ggaaagcgat cctccatctg cagcgacatg
    11941 aacatgcgcg gcatgagcgg aaagtgaaag ttcatgtgcg attcgtcgcc cttgccaaag
    12001 taagcagccg catcctccgg ccattgattg gcctccgcga gcagcattcg gtttggaaac
    12061 tttgcctcca cgtgagcgcg cagcttgcgg agataatcat gggtctctgg cagattttcg
    12121 cagatagtgc cctcgcgttc gtaaaggtac gggacagcgt caagacgaag accgtcaacg
    12181 ccaagggcga gccaaaaatc gcaaaccttc tcgaccgcgt cgtgcacggc cggattatcg
    12241 aaattcaagt ccggctgatg cgaataaaag cgatgccaat agtaagcttt tgcgacggga
    12301 tcccacgccc agtttgaggt ctcgaaatct ttaaagatga tccgcgcatc cttgtatttc
    12361 tccggtgtgt cgctccagac atagaagtct ctgtaggcaa gatcgtcgtt gtgaccgcgg
    12421 gtgccgaccg cgggtcccgg tgtcgatcct gcccgcctga ggtcaccgat cccggctgta
    12481 gctatagcgc gccgcgattt ttggaaccac ggattctgat cggacgtgtg attgatcacc
    12541 aattcggtaa tgactcgcaa attgcgttca tgcgccgcgt caagaaacgc gcggaaatca
    12601 tcgagcgtgc cgaagttcgg attaacgtcg aaataatcgg cgatgtcgta accgtcatcc
    12661 cgaagcggtg agggataaaa cggcaggagc cagacggcgg tagtgccgag ttcctggaga
    12721 taatccagct tttcaatcag gccacggaaa tcccccatgc cgtcgccgtc gctgtcgcag
    12781 aacgtcttga catgcagttc gtaaataacc gcgtccttgt accacagcgg atcgatcgtt
    12841 gtttcgcccc cattcgcggc catgcggcta acctttgccg cacaaatggc gccgcgcaat
    12901 tgcgacgatc attacgccgc gacggccgtg gccgccacca actttaaatc cgcccaatga
    12961 actgggattc ctttaacgct gccctaccca catgacctgg ctggctatgc attgccagtt
    13021 gccgccccga tccatccaag tgtcggtaaa gcgataggta cgatcaaagg ccttgccatc
    13081 tttgcctgtg cctttttcgt tggctgtccc cacgacaact gcaacgccgg tgccgtacat
    13141 gtgtacatcg agtttctcgg ccttggttga tgtgtaggtg tctttgtcgg ctttcacttc
    13201 agccaacatc gcacggcgat ttacaacttt gcccttggaa cttactccga tgaaatcagg
    13261 agccaccatc gattcgatgg ttgcggtgtc gtgtctggcg atagcagccg accagcgttt
    13321 ttcgttgtcc ttcaacatgg agctgacgct cattttcttt cctgtcgcag gggcaggcga
    13381 ggtggtcgca
//
LOCUS       DASQUQ010000039         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_912452, whole genome shotgun sequence.
ACCESSION   DASQUQ010000039 DASQUQ010000000
VERSION     DASQUQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912452"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VE486_01120"
     CDS             <1..676
                     /locus_tag="VE486_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZA37719.1"
                     /translation="PVLYLHDGQNVFDAATAFAGVEWGVDETAERLIRRKLIEPLIIV
                     AVANMGDDRIHEYAPTRGVIDARAKRKKRSRGLARLYGHFLIDELKPYIDRKYRTRPG
                     REFTGLGGSSLGGLVTLAIGILYPQVFSRLIVMSPSIWWDDFAIYRLVDSIEQKPPLK
                     IWLDTGTAEPGWEQARKLCERLIEKGWRVSDDLAYLETEGADHSEGAWAVRVAPALRF
                     LFPFRL"
     gene            complement(827..1627)
                     /locus_tag="VE486_01125"
     CDS             complement(827..1627)
                     /locus_tag="VE486_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37720.1"
                     /translation="MLRNWRTTAGFCWLSAFVAVASARPSTATPAFRFDRDTFAFANQ
                     TVFEYREGIPYARKKSKEQADRYNRRCFVLCRTATQFYKFARFDPRGAPLDDKALAAR
                     IRSVTRRQAWAAPLPEDQRVVFPGYANLREMSKARGKLLRANVGLGWTVYLRPSNGRI
                     VLLQDRRYQEQVHAKLNESLARGEVFVGYLSTFPRITVNHAILVYKRKSVSPETGIER
                     YAAYDPNHPEAPRELTWSGKTRAFAYQKDVDFVGGFVRVYQVYGKWLQ"
     gene            complement(1633..2619)
                     /locus_tag="VE486_01130"
     CDS             complement(1633..2619)
                     /locus_tag="VE486_01130"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZA37721.1"
                     /translation="MDLQPLDFEKPVLELERRLQDLKNHSDEHDVDLDSAVKAVEGKL
                     RETRRQIYSNLTAWQRVQIARHVQRPFALDYIERCFTNWIELHGDRRFADDKAMPSGL
                     AMLGTQRCMVIAHQKGRNTKENVMRNFGCAHPEGYRKALRLMRLGEKFGMPVISLIDT
                     PGAFPGIGSEERHISEAIAVNLREMMRLRVPVIAAVIGEGGSGGALGIGVADRVLILE
                     NAYYSVISPEACSAILWRDRRHAAEAAEALKLTAQDLLKLDVVDEIVPEPEGGAHRDY
                     DSAVENLGRALRQNLQDISKIPIDELLKKRYEKFRRIGIFTESKTTAASDRP"
     gene            complement(2717..>2933)
                     /locus_tag="VE486_01135"
     CDS             complement(2717..>2933)
                     /locus_tag="VE486_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37722.1"
                     /translation="KRVGLGSKEYIGSTRWIRFAGAPAYTLYSTPDAAHPNLDQPPPP
                     SGLGLAASDVEELSGLVNNGTSVTITD"
BASE COUNT          638 a          809 c          827 g          659 t
ORIGIN      
        1 tccggttttg tatttgcatg acggccagaa tgttttcgat gctgcaaccg cattcgcggg
       61 cgtggaatgg ggcgttgacg agacagccga gcgattgatt cgcagaaagt taatcgagcc
      121 gctcatcatc gtagctgtcg cgaacatggg cgacgatcgg atccacgaat atgcgccgac
      181 gcgcggcgtg atcgatgccc gggcgaagcg aaaaaaaagg agccgcggac tcgcgcgctt
      241 gtacggccac tttctcatag acgaactcaa gccctacatc gaccggaaat atcgaacaag
      301 accaggacgc gaattcaccg ggttgggcgg ttcatcactc ggtggtctcg ttacgctggc
      361 gatcggaatt ttatacccgc aggtgtttag ccggctcatc gtcatgtcgc cctcaatctg
      421 gtgggacgat ttcgcgattt accggctcgt ggacagcatc gaacaaaaac ctccgttaaa
      481 aatctggctc gatacgggca cggccgagcc gggatgggaa caggcgcgca aactgtgcga
      541 acgtctcatc gagaagggct ggagagtttc cgatgatctt gcgtatttgg aaaccgaagg
      601 tgccgaccac agcgaaggag cctgggccgt gcgggtcgcg cctgcgctgc ggtttctttt
      661 tccgttccgt ctgtagttca cctgccgact ggcgggaccg gcggaggatt gggcggcaac
      721 ggctccgggc gtggcactgg cttgggtgtt ggtcccggcg ctggcggcat cggttcaggg
      781 ggtgctgggc tatctggcat tggtgtgctc cagtaatgaa tcgtttttac tggagccact
      841 tgccgtaaac ctggtaaacg cgcacgaagc ctcccacgaa atccacatct ttttgataag
      901 caaaagcgcg cgttttccca gaccaggtaa gctcacgcgg tgcttcgggg tgattgggat
      961 cataagcggc ataccgctca attccggtct ccggggacac ggactttcgt ttataaacca
     1021 gaatcgcgtg attgaccgtg attcgaggga aagtgctgag atatccaacg aatacctccc
     1081 cgcgcgccaa tgattcattc agctttgcgt ggacctgttc ctggtatcgc cgatcttgca
     1141 ggagaacaat ccggccgttg cttggccgca agtaaacggt ccaaccgaga ccgacgttgg
     1201 ctcgcaaaag tttcccgcgt gccttgctca tttcgcgcag attagcatag ccgggaaaaa
     1261 caacgcgctg atcctcgggt aaaggagcag cccaggcttg tcgtcgtgta accgaacgaa
     1321 tgcgtgccgc taacgcttta tcatccaacg gagcgccgcg tggatcgaag cgggcgaatt
     1381 tgtagaactg cgtcgccgtc cggcataaga cgaaacagcg ccgattgtac ctgtctgcct
     1441 gttctttcga ttttttccgg gcataaggga tcccttcgcg atattcaaag acggtttggt
     1501 tggcgaatgc gaaagtgtcg cggtcgaaac gaaacgccgg cgtcgccgtg ctcggacgcg
     1561 ccgaggcgac ggcgacaaat gcgctgagcc agcaaaaacc cgcagttgtg cgccaattgc
     1621 gaagcatctc gatcaagggc gatccgaagc ggctgtcgtt ttgctttcgg tgaaaatgcc
     1681 gattcggcga aatttttcgt agcgcttctt tagcagctca tcaatcggaa ttttggaaat
     1741 gtcctgcaga ttctggcgca gcgcccgtcc caaattttcg acggcagaat cataatcgcg
     1801 gtgagcgcct ccctccggtt cgggcacaat ttcgtcaacc acgtcaagtt tcaacaaatc
     1861 ttgcgcggtt agttttagcg cttcggccgc ctcggctgca tggcgtcgat ccctccacaa
     1921 aatcgccgaa cacgcttccg ggctgatcac ggaataatag gcgttctcaa ggatcaatac
     1981 ccgatctgcg accccaatgc cgagcgcgcc gcctgatccg ccctcgccga ttacggcggc
     2041 gataacaggc acacgcagtc tcatcatctc ccgaagattt accgcgattg cttccgaaat
     2101 atggcgttcc tccgatccaa tgcccggaaa tgcgcccggc gtatcgatca gtgagatcac
     2161 gggcatccca aacttttctc ccagtcgcat cagtcgcaac gccttgcgat aaccttcggg
     2221 atgggcgcag ccgaaattgc gcatcacatt ttccttggtg ttgcgccctt tttggtgagc
     2281 gatcaccata cagcgctgcg tgccgagcat ggcgagaccg ctcggcatgg ctttgtcatc
     2341 ggcaaaacga cgatcgccgt gcagttcaat ccagtttgta aaacaccgtt cgatataatc
     2401 gagagcaaac ggccgctgca catgacgtgc gatttggaca cgctgccagg cagtcaggtt
     2461 actgtagatt tggcgccggg tttcgcgcag cttgccctca actgccttta ccgccgaatc
     2521 caggtcgacg tcatgttcgt cagagtgatt ctttagatct tgcaagcgcc gttccagttc
     2581 aaggaccggt ttttcaaaat cgagcggctg cagatccatg agaggaaagt taaaagagtt
     2641 aaaacgttac aaagttacag gagcaaactc gctcatgacg tttcacgacg taacggtttt
     2701 aactttttca ccaatttcaa tcggtaatcg tgacagaggt cccgttgttt acaaggccgc
     2761 ttagttcctc gacatcagag gcggccaggc caagccctga tggtggcggc ggctgatcga
     2821 gattcggatg cgcggcatcg ggcgtggaat acaatgtgta agccggcgct ccggcaaacc
     2881 gaatccagcg ggtgctgcca atgtattcct tgctcccgag gcccacgcgt ttt
//
LOCUS       DASQUQ010000040         4058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1000343, whole genome shotgun sequence.
ACCESSION   DASQUQ010000040 DASQUQ010000000
VERSION     DASQUQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000343"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VE486_01140"
     CDS             complement(<1..482)
                     /locus_tag="VE486_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37723.1"
                     /translation="MRFLLNLPPAISFLVVSAVTTAIALAGIRFVRKKYAAEVLKENH
                     EVAAIIFNAFGLLYGVVVAFVVFVTWSGYDEATKSLQMEANEADDVFHITEAFPDPAR
                     KTIQQALMDYVVSVYNDELKRMSQGDISLHSNRAMAKLITDFYRMDEKSVPNRELYSE
                     S"
     gene            complement(489..2681)
                     /gene="recQ"
                     /locus_tag="VE486_01145"
     CDS             complement(489..2681)
                     /gene="recQ"
                     /locus_tag="VE486_01145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HZA37724.1"
                     /translation="MDFRAALKKHFGYEAFRPLQEAIITDALAGRDVFALMPTGGGKS
                     LCFQLPALMRDGLTIVVSPLISLMKDQVDALQTSGIAATFLNSTLDRQEAVARWRGLH
                     RGEYRMLYVAPERLMLDTFLERALNWNIAQIAIDEAHCISEWGHDFRPEYRELKKLRT
                     HLPDVPVMALTATATERVRADILKQLKLRDPRCYVASFNRPNLTYRVVPKSASYEQLL
                     AFIRGRPNESGIVYCASRKSADSLAARLNEDGVSAKPYHAGLTTKERTRNQELFLRDD
                     ARVITATIAFGMGINKPNVRFVVHYDLPKNLESYYQETGRAGRDGLPSECVLLFSASD
                     VAKQLHFIDEKSESEARIAREQLRQMVHYAETRECRRATLLRYFGEQFSQQSCDGCDN
                     CLQPRETFDGTVPAQKFLSCVHRIHRKSGFGFGVNHIVEVLTGADTQAIRQRRHNEVS
                     TYGIGRELKRDAWQAIGRELLRLGLIECAPGKFATLTLTQAGLDALRQRTPITLTKQI
                     EIADKTGKARAGAIECDELLFERLRVLRRKLADDRNVPPYVIFSDVSLREMARTYPGS
                     VNAFRQIPGVGEQKLKDFAEIFLSEIRDYLANNSRRTFADDVDVSFPQRRPGLNDSQA
                     ETLRRCCRGESVDEIARARGFVRSTIYGHLLAAIECGKIGAEARDRFFTSTQEKEIAA
                     AFRQVTVGTLTDVSALLGGKYDIGLLRMFRAFTARRFGGRTVEGPLRN"
     gene            complement(2722..3618)
                     /locus_tag="VE486_01150"
     CDS             complement(2722..3618)
                     /locus_tag="VE486_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HZA37725.1"
                     /translation="MLDLTVTEELSLPVSIERAREACERAITQLDWIIREQSPTALLA
                     REKGLFGMDWLGTKTLTAPADLSLFLSESDNGARVRLNATLMYGKTSAKLFRPLLQGK
                     LNKIRERLLAEVPGSRPVSVAPPLPEPPPSPEPPPSSRVTGRIFISYRRRDSADVTGR
                     IYDRLVNHFGEGAVFQDVEDIPLGMDFSEYIQEVVGKCDAFLAIIGNQWLDAADKGGG
                     RRLDNPADNVRIEIESALLRKIPLIPVLVSGAEMPAADALPESLRKLAMRNATRVRPN
                     PDFRPDMTRLIEGLEELFAQNR"
BASE COUNT          876 a         1182 c         1108 g          892 t
ORIGIN      
        1 gattcgctgt agagctctcg gttgggaacc gatttctcgt ccatccgata gaaatctgtt
       61 atgagctttg ccattgcccg gttggaatgg agacttatgt cgccctgtga cattcttttc
      121 agttcatcat tataaaccga aacgacataa tccatgagcg cttgctgtat cgttttacgt
      181 gccggatcgg ggaacgcttc cgtaatgtga aaaacgtcgt cggcctcgtt ggcttccatt
      241 tgcagacttt tcgtggcctc gtcgtatcca ctccacgtga cgaacacgac aaaggcgaca
      301 accaccccgt aaagcagccc gaacgcgtta aagattatag cggctacttc gtggttttct
      361 tttaacactt ccgccgcata tttctttcgc acaaaacgaa tcccggccaa tgcgatcgca
      421 gtggtcacag ccgagaccac caaaaatgaa atagctggcg gcaaattaag caggaatcgc
      481 ataagcagtt agtttctcag aggtccttcg acagtgcgac ctccgaatcg tctcgcggta
      541 aatgctcgaa acatccgcaa cagcccgata tcgtatttgc caccgagcag cgcgctcaca
      601 tcggtaagag ttccaacggt tacctggcgg aatgcagccg cgatttcctt ctcctgggta
      661 gaggtgaaaa atcgatcccg cgcctctgcc ccgattttgc cacactcgat cgcagccagc
      721 agatgcccgt aaatcgtact gcgtacaaag ccgcgcgcac gcgcgatttc atccaccgat
      781 tcgcctctac aacatcgccg caaagtttcg gcctgactgt cgttcaagcc gggacggcgt
      841 tgcgggaagg agacatcgac atcgtcggca aatgtccggc gcgagttgtt tgcaaggtaa
      901 tctcttattt cactgaggaa gatctcggcg aaatctttca atttttgttc gccgacgccc
      961 gggatttgcc ggaacgcatt cacactcccc ggataagtcc gtgccatttc ccgcagcgaa
     1021 acgtctgaga agatcacgta aggtggcaca ttgcgatcgt cagccagttt gcgtcgcagg
     1081 acccgcaggc gttcgaacaa cagttcgtcg cattcaatcg cgcccgcacg agctttcccg
     1141 gtcttatcgg caatctcgat ctgcttcgtc agcgtgattg gtgttcgctg gcgtaaagcg
     1201 tccagaccgg cttgggtgag cgtcaacgtg gcgaacttgc cgggcgcgca ttcgatcagt
     1261 cccagccgca aaagctcgcg cccgattgcc tgccaggcat cgcgcttcag ttcccggcca
     1321 atcccgtaag tcgatacttc gttatggcgc cgttgccgga tcgcttgagt atctgcgccg
     1381 gtgagcactt cgacaatgtg attcacccca aagccgaacc cgctcttcct gtgaattcga
     1441 tgaacgcagg agaggaattt ctgtgccggg acggttccat cgaaagtttc gcggggttgc
     1501 aaacaattat cgcagccatc gcaggattgc tgcgaaaact gttcgccgaa atatctgagt
     1561 agcgtcgcgc gcctgcattc acgtgtctcg gcgtaatgga ccatctggcg gagttgctct
     1621 cgcgcgatcc gcgcttcgct ctcactcttt tcgtcaatga aatgcaactg cttggcgacg
     1681 tcgctcgcgc tgaaaagcaa cacgcattcg ctgggcagcc cgtctcgccc ggcgcgtccc
     1741 gtttcctgat aatagctctc cagattcttt ggcagatcgt aatgcacgac aaaccgcaca
     1801 ttcggtttgt tgatgcccat gccgaacgcg atcgtcgcgg taatgacgcg tgcatcatcc
     1861 cgaagaaaaa gctcctgatt ccgggtccgt tctttggtcg ttagtccggc gtgatacggc
     1921 ttcgcgctta cgccgtcttc attcaggcgc gccgcaagcg agtccgcgct ttttctgctt
     1981 gcgcaataaa cgatgccgct ttcgttgggc cggccacgga tgaatgccag cagttgttcg
     2041 tacgacgccg atttcgggac gacgcgataa gtcagattcg gccggttaaa gctggcgaca
     2101 tagcagcgcg gatcccgcag cttcagttgc ttaaggatat cagcccgcac acgctcggtt
     2161 gcggttgcag tgagcgccat taccggcacg tcgggcaaat gcgtgcgcaa cttctttagc
     2221 tcacgatatt ctggccggaa atcgtgcccc cattcgctaa tgcaatgcgc ttcatcaatc
     2281 gcgatctgcg cgatgttcca gttcagcgcg cgctccaaaa acgtgtcgag catcagccgc
     2341 tccggcgcga cgtagagcat ccggtattcg ccgcgatgaa gcccgcgcca gcgcgccact
     2401 gcttcctgtc gatcgagtgt cgaattcaaa aacgtcgccg cgatcccgct cgtttggagt
     2461 gcatcgacct gatccttcat cagcgaaatg agtggcgaca ccacgattgt cagcccatcg
     2521 cgcatcaagg ctggcaattg aaaacaaagc gatttcccgc caccagttgg cattagcgca
     2581 aacacatcgc gacccgcgag cgcgtccgtg atgattgctt cctgcaacgg gcggaacgct
     2641 tcgtaaccga agtgtttttt tagggcggcg cgaaaatcca tgtgactcgt tttaattgcc
     2701 gatcgcgcct ccgaaaacgg attagcggtt ttgcgcaaag agctcctcga gcccttcgat
     2761 gagccgcgtc atgtcggggc ggaaatctgg attcggccgg acacgagtgg cgttgcgcat
     2821 ggcgagtttg cgcaacgatt ccggcagcgc atccgcagcg ggcatctcgg ccccgctaac
     2881 aagcactgga ataagaggga tcttgcggag cagagcgctc tcgatctcga tgcggacgtt
     2941 gtcagcagga ttgtccaggc gccggccgcc gcccttgtca gcggcatcga gccactgatt
     3001 tccaatgatt gcgaggaacg cgtcgcattt accgaccact tcctggatat actcgctgaa
     3061 atccattcct aggggaatgt cttccacgtc ttgaaacacg gcgccctctc cgaagtgatt
     3121 gaccaggcga tcgtaaatgc gcccagtcac gtcagcgcta tcgcggcgcc ggtacgaaat
     3181 gaagatgcgg cctgtaacgc gagaagatgg cggaggttct ggagatgggg gtggctctgg
     3241 gagaggcggc gcgactgaaa ccgggcgcga cccggggact tcggccagga gccgctctcg
     3301 gattttattg agcttgccct gaaggagcgg acggaaaagt ttcgccgagg tcttgccgta
     3361 cattagtgtc gcattcaacc gtactcgcgc gccattatca gactcgctca gaaatagtga
     3421 caggtccgcg ggcgcagtga gcgtctttgt cccaagccag tccatgccga aaaggccttt
     3481 ctctcgggcc agcaacgcgg tcggcgattg ttcccgtatg atccaatcca gttgcgtgat
     3541 cgcgcgttcg caggcctcgc gagcgcgctc gatcgaaacc ggcagactca gttcttccgt
     3601 tacggtcagg tccagcattt cgggatttcc gcgcaggtgt tatccggcga atggaatggc
     3661 aggagactag cataatcttt tcctcgctat caagacatga tgcaaacggc tgaagaagga
     3721 tgcggctggc gccattgccg actacacccg agccattgaa ctcggctcca caaacccggc
     3781 tgcttacaac aaccgcggca acgctagatc ggagagtaat gaccgagatg gcgcaattgc
     3841 ggatttcact cgggcaatcg aactcaaacc cgattacgca cgcgcctatt acaatcgcgc
     3901 tgtcacgaag caggccaaag gcgacatgac cggtgccaag gcagacttca agaccgccga
     3961 gaaactcgat ccggaattgg ccagcgaaga gtcggccgag tcggccgcgg attcaaaaaa
     4021 caatcgtcct tcgggagtga caaccgtcag tctcctgg
//
LOCUS       DASQUQ010000041         6752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_638592, whole genome shotgun sequence.
ACCESSION   DASQUQ010000041 DASQUQ010000000
VERSION     DASQUQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638592"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2216)
                     /locus_tag="VE486_01155"
     CDS             complement(<1..2216)
                     /locus_tag="VE486_01155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA37726.1"
                     /translation="MSKCDLHIHSRYSARSEEWLFRRFDFPDSYSDPKELHRQLLGHG
                     MDYVTITDHDTIEGCLQIADLPHTFISEQVTTYFPQDQCKLSILVWGISEQQHREIEE
                     VRDNIFELQGYLQTAQIAHAVAHPLYSVNGKLEASHLERLILLFKHFEGINGLRDALL
                     SNLVQTLLSQLTPDKIEMLANRHNLAPTHAEPWKKILVGGSDDHGGQFVASAFTEAPG
                     ADSAEKFLASVRSGNCSPRGRGGTPLALSHGFYNTLVRFIEDRFHEKLGPSAGLLEKM
                     FSRFMEGRDPTEFTLGEKASLAGQAVVSGKIFELLKPANVSLWKELSGYFARPEVKAK
                     IARQLDKVSEPERRTFLLANMVSEQLAFRFFEKFVQQLSSGNMVESMQALSAIAPILV
                     ILTPYIYGFHSQAPSRKWLRGIFRELTGEVPVALQNHKRAWFTDTLDDVNGVATTIRK
                     MTAAGVAAGKELIVVVSRGELQTDDIPIKNFKPIGEFELPEYELQKLSFPPILQILDY
                     IQREKFTEIIVSTPGPVGLTGLLAAKMLNLQTSGIYHTDFPQYIRILTEDGFLESVAW
                     RYMHWFYGQLDTVFVNSEEYRQSWIQRGFDPAKLRIFPRGLDTNLFSPTRREPAFWEK
                     FGAGNAQVRLLYVGRISKEKDLDILAEAYRRLRGEDLPVQLFVVGHGPYSEAFAESVP
                     EAFFTGYLTGRELATAYASADIFVFPSTTDTFGNVIIEAQASGVPVVVSDSGGPKE"
     gene            complement(2219..3010)
                     /locus_tag="VE486_01160"
     CDS             complement(2219..3010)
                     /locus_tag="VE486_01160"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZA37727.1"
                     /translation="MEFLPVVAAPSVSRDFLIVSIHDVAPATRDAVEKIISELAHIGV
                     PVWSLLVVPDYHHQGLATKDRQFVSWLRELESDGHEIVIHGFFHERPRHANETIRDKF
                     FTHFYTADEGEFYDLDYDEALRRISTARDAFRASGLKPRGFVAPAWLLSKEAERAARD
                     AELEYTTRLHAVRDLRSGEEFAARSIVYSVRASWRRAVSRSWNATLFRLLQSSPLLRI
                     SIHPPDYSHPAIWRQIADLIEATAGSRTTTTYQDWIAQQRLRRGV"
     gene            complement(3121..3531)
                     /locus_tag="VE486_01165"
     CDS             complement(3121..3531)
                     /locus_tag="VE486_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZA37728.1"
                     /translation="MKSFWLVKQEPSSYSWSDFLAEGETAWTGVRNYTARNNLRKMKT
                     GDEVLFYHSGEEKAVVGIARVTRPAYPDSTAKEGDWSAVDLAPVKSLRQPVTLRQIKS
                     DSRLKQIPLVRQSRLSVMPLAKSEFREIVQMSMG"
     gene            3732..4376
                     /gene="tmk"
                     /locus_tag="VE486_01170"
     CDS             3732..4376
                     /gene="tmk"
                     /locus_tag="VE486_01170"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZA37729.1"
                     /translation="MRRLPFISFEGSEGCGKSTQVKRLAARFEHFGIPFLVTREPGGT
                     PIGESIRELLQFVPHNAGMTPETELLLFEASRSQLVREIIKPALERAVCVIADRFFDS
                     TTAYQGAARKLDRQIVEQLNAFVIGDCIPDITFVLDIDAETARFRMQQPRRADRMEQQ
                     PAEFYEQVRQAYRELAAREPNRVVLIDGSRGADEIENEIWKILCSRFPNLNTRH"
     gene            4391..4813
                     /locus_tag="VE486_01175"
     CDS             4391..4813
                     /locus_tag="VE486_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZA37730.1"
                     /translation="MAKQRDGETARKPIRHFRDLDLYQNALSTGLRVYELTKRFPVEE
                     RYALTDQIRRSSRSVCANIAEAWRKRRYEAAFVSKLSDAEGEAAETQVHLEFAFHHRY
                     VSRDEFLSVDDAYEKIIGQLVKMIDKPEKWLIKNARHR"
     gene            4905..5879
                     /locus_tag="VE486_01180"
     CDS             4905..5879
                     /locus_tag="VE486_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZA37731.1"
                     /translation="MAFSRTSALEYLRRAHGQNRLAHAYLISGPPGSGKQVLAAELTS
                     LVNGTNVNDVFSTKAREIFVARPESKSRRIVIEQIRDLEHALQMRASNGRRKVVIISD
                     ADRLQSQAANAFLKTLEEPPKDSLMLLLSALPEALPETILSRCIAIPLAPHDQPQRKS
                     AEEEKLVKLLQQSARAQRWNIQYAYRLAQEFQRLLRAIREQARRETDEAFKKEQARYK
                     DATDGVWLDEREQYYKALAESLYLQRRAGLIETLFAWWSDVLRARNNVEHHNLPDAKE
                     ETSVLAKRFSTPEILQRVRRLEELRDHLGRNIQEALAIEVAFLAIFTG"
     gene            5998..6741
                     /gene="fabG"
                     /locus_tag="VE486_01185"
     CDS             5998..6741
                     /gene="fabG"
                     /locus_tag="VE486_01185"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZA37732.1"
                     /translation="MRFENQIAIVTGAGRGIGRAIAVHLASEGARVACVSRTEENAKR
                     TADEINIIRADAAKAYAVDVADHAAVQKIGAQILKDFMKIDILVNNAGVTRDGLAMRM
                     SIEDWDAVVNTNLRGAFNFTQAIVRSMTKQRSGRIINISSVIGLIGNAGQTNYAASKA
                     GLIGFTKSLAKELASRNITVNAVAPGFITTDMTADLSDEIKKSIHSKIPLGRTGGPEE
                     IASAVAFLGSAEANYITGQVLCVDGGIVM"
BASE COUNT         1572 a         1842 c         1879 g         1459 t
ORIGIN      
        1 aactctttgg ggccgccgga atcggaaaca accaccggca cgccggatgc ctgtgcttcg
       61 atgatgacgt tgccgaacgt atcggtcgtg ctgggaaaga caaagatgtc ggcggacgcg
      121 taggccgtgg ccaattctct accggtgaga tagccggtaa aaaatgcctc gggaaccgat
      181 tcggcaaacg cttcggagta agggccatga ccgacgacaa aaagctgcac gggaagatct
      241 tcaccgcgca atcgccgata ggcctccgct aaaatatcga gatctttttc tttcgatatc
      301 cggccgacgt agagcaaacg cacctgagcg ttgcccgcgc caaatttttc ccaaaaggcc
      361 ggctcacgtc gggtcgggct aaacagatta gtatcgagac cacgcgggaa aattctcagt
      421 tttgccggat cgaagccgcg ctggatccag ctttggcgat attcttccga gttgacgaag
      481 acggtgtcga gctggccgta gaaccaatgc atgtaacgcc atgccacgct ttcgagaaac
      541 ccatcctcgg tcagaatccg gatgtattgt ggaaagtctg tgtgataaat gccgctcgtt
      601 tgcagattga gcatcttggc cgcgaggagc ccggttaatc cgaccggccc cggcgtgctg
      661 acgataattt cagtaaattt ttcccgctga atgtaatcga gaatctgaag aatcggcggg
      721 aagctgagct tttgcagctc atattcgggc aattcgaatt cgccgatcgg cttgaaattt
      781 ttgatcggga tatcgtctgt ttgcagctcg ccgcgtgaga cgaccacgat taactctttt
      841 ccagcggcta cacccgccgc ggtcatcttg cgaatcgtcg tcgcgacacc attgacgtcg
      901 tcgagagtat cggtgaacca cgctcgtttg tgattttgca gcgcgaccgg cacttcgccg
      961 gtcaactctc tgaagatgcc gcgaagccat tttcgcgacg gcgcctggct atgaaaaccg
     1021 tagatgtatg gcgtgaggat taccaggatc ggcgcgatcg cgctgagagc ctgcatgctt
     1081 tcgaccatgt tgccgctgct gagctgctga acgaattttt cgaaaaaccg gaacgcgagc
     1141 tgttcggaca ccatgtttgc caggagaaat gtgcggcgct ccggttcgct gactttgtcc
     1201 aattgcctgg cgattttcgc cttcacttca ggtcgcgcga aataaccaga gagctctttc
     1261 caaagcgaga cgttcgcagg cttcaatagt tcgaaaatct ttccagacac tacagcttgc
     1321 ccggccaggc tcgctttttc tccgagcgtg aattccgtcg gatcacgccc ctccatgaag
     1381 cgcgaaaaca ttttttccag caatccagcg ctcggcccga gtttttcgtg gaaccggtct
     1441 tcgatgaaac gcacaagcgt gttgtagaaa ccgtgcgaca gggcgagggg agtcccccca
     1501 cggcctcgtg gactgcaatt accgctacga acggacgcaa gaaatttttc cgcggaatct
     1561 gcgcccggcg cttcggtaaa agccgaagcg acaaattgtc cgccgtggtc gtccgagccg
     1621 cccaccaaaa tctttttcca cggctccgcg tgggtcggcg caagattatg gcgatttgcg
     1681 agcatctcga tcttgtcggg cgtcaattga ctgagaaggg tctggacgag attgctcaaa
     1741 agcgcgtcac gcagaccgtt gattccctcg aaatgcttga aaagcaggat gagccgctcg
     1801 agatggctcg cctcgagttt gccgttgacg ctataaagag gatgcgcaac cgcatgcgcg
     1861 atttgtgcgg tctggagata gccttggagc tcaaagatat tatcgcggac ctcttcgatt
     1921 tcccggtgct gctgctctga aattccccaa acgaggatgc tcagcttgca ctgatcctgc
     1981 ggaaaatatg ttgtaacctg ctcgctaata aacgtgtgag gcagatccgc gatttgcaga
     2041 cagccctcga tcgtatcgtg atcggtaatc gtcacgtaat ccatgccgtg tcccagtaac
     2101 tgtcggtgca actcctttgg atcggagtag ctatcgggaa aatcgaaacg acggaacagc
     2161 cattcctccg agcgcgcact ataacgcgag tggatgtgaa ggtcgcactt actcatgcct
     2221 agactcccct tcttaacctt tgttgagcga tccagtcctg gtatgtggtc gttgttcttg
     2281 atccggctgt cgcttcgata agatcagcga tctgccgcca aatcgccgga tgcgaatagt
     2341 cgggcggatg aatgcttatg cgcaagagcg gactgctctg caaaagccgg aagagcgtag
     2401 cgttccagga acggctgact gcgcgccgcc aactggcccg gacgctataa acaattgatc
     2461 gggcagcgaa ttcttcgccc gaacgcagat cgcggactgc gtgtaaccgc gtcgtgtatt
     2521 ccaattcggc atcacgcgca gcacgctcgg cttccttgct gagcaaccag gcaggcgcaa
     2581 caaaaccgcg cggcttcaag ccgctggcgc gaaatgcatc ccgtgccgtt gagatccggc
     2641 ggagggcctc gtcatagtcg agatcgtaaa actcgccttc gtctgcggtg tagaagtggg
     2701 taaaaaactt gtcgcgtatc gtttcgttcg cgtgacgagg ccgttcgtga aaaaacccgt
     2761 ggatgacaat ttcatgtccg tccgattcca attctcgcag ccaggaaacg aactgccgat
     2821 ctttggtggc aaggccttga tgatgataat cgggcacaac cagaagagac caaaccggca
     2881 cgccgatgtg agcgagctcg gagataatct tttctaccgc gtctcgcgta gcgggagcga
     2941 cgtcgtggat cgacacaatc aggaaatcgc gggagacgct tggcgccgcc accactggta
     3001 aaaattccat tgtcgctgcc gattccatcc tgccttctta tgtaataatt cttggccttg
     3061 gtggagcttc caacaaggca aattcgagca gttgaatcgc ctgcggaccg gccgagccac
     3121 ctatcccatg ctcatctgca caatctcgcg aaactcggat ttagccagcg gcataaccga
     3181 caaacgtgat tgtcgaacca ggggaatctg tttcaggcga gaatcgcttt tgatttggcg
     3241 cagggttact ggctgacgca gagatttgac tggcgcgaga tcgacggcgc tccagtcgcc
     3301 ctcttttgcc gtagaatcgg gataagccgg tcgtgtgacc ctggcgatac cgaccacggc
     3361 tttttcctca ccgctgtgat aaaaaagcac ttcatcgccg gttttcattt tgcgcagatt
     3421 atttcgcgcg gtgtagttac gcacccccgt ccacgcagtt tctccctcgg ctaaaaaatc
     3481 cgaccacgaa tacgagctcg gttcttgctt caccagccag aagcttttca tatggcaaag
     3541 taatccagcg aagttgtggg cggcaaattt cgctcaatgc tgcagcgtgc gctgtcctca
     3601 gcgcactgac ttgtgcatgg cggcacgctg ggtgatccgc tgagacagcg gacactacag
     3661 cacatggggc gactggatgg acagccgctc tttattcgac aattgcgccg ctttatttca
     3721 caatcgatgt gatgcggcgg ctgccattca tctcgtttga aggctctgag ggatgcggga
     3781 agtccaccca ggtgaaaaga cttgcggccc ggttcgagca tttcggcatt ccatttctcg
     3841 tgacacggga gccgggcggc acgccgattg gcgaatcgat tcgcgagctt cttcaatttg
     3901 tgccgcataa cgccggcatg acgccggaga ccgagctgct cctgtttgag gccagccgaa
     3961 gccagcttgt ccgcgaaata attaagccag cgctggagcg cgccgtttgc gtgattgccg
     4021 atcgtttctt tgattccacg actgcctacc agggcgcggc gcgaaaatta gaccggcaaa
     4081 ttgttgaaca gctgaacgca ttcgtgatcg gcgattgcat tcccgatatc actttcgtgc
     4141 tggatatcga tgcagaaacg gcgcgtttcc ggatgcaaca accgcgcaga gccgaccgga
     4201 tggaacaaca acctgccgaa ttttatgagc aagtgcgcca ggcgtatcga gaactggccg
     4261 cgcgtgaacc caatcgcgtt gtgctgatcg acggctcgcg cggcgcggat gagattgaga
     4321 acgagatctg gaaaatcctt tgcagtcgct tcccaaatct gaacactcgt cactgacaat
     4381 gatggacgaa atggcgaaac agcgagacgg cgaaacggcg cgaaaaccga tccgccactt
     4441 ccgagatttg gacctatacc agaatgcgct ttcgaccggc ctacgcgttt acgagttaac
     4501 caagagattt cccgtcgaag aacgctacgc gctgactgat caaatccggc gatcttcgcg
     4561 ctcagtgtgc gccaacattg ccgaagcctg gcgaaaacgg cgatatgagg cggcctttgt
     4621 ctcaaaactg agcgatgcgg aaggcgaagc tgcggaaaca caagtccatt tggagttcgc
     4681 atttcaccat cgctacgtct ctcgcgacga atttctaagc gttgatgatg cttacgaaaa
     4741 aataatcggc cagctcgtga agatgatcga caagcccgag aagtggctga tcaagaatgc
     4801 gcgacaccga tgaagtgcgg cgaactacag aaatggggga acagagagaa ggacgactcc
     4861 gccgattcgc ccattcgccg cctccccgat tctttctaat tgacatggct ttctcccgca
     4921 caagcgctct tgaatacttg cgccgcgccc acggtcagaa ccggctggcg cacgcctatt
     4981 tgatctccgg accgcccggc agcggaaaac aggtgctagc ggcagagctg acgagtctcg
     5041 taaacggaac aaacgtgaat gacgtcttct ccacaaaagc tcgagagatt tttgttgctc
     5101 gacccgaatc gaaatcccgg cgcattgtga ttgagcagat tcgcgacctt gagcatgccc
     5161 tgcaaatgcg cgcctcaaat ggccggcgca aagtcgtgat tatttccgat gccgaccggc
     5221 tgcaatcgca ggcagcaaac gcgtttctga aaacgttgga agaaccgccg aaggattctt
     5281 tgatgcttct tctgagcgcc ttacccgaag cccttccgga aacaattctt tcccggtgca
     5341 tcgcaattcc acttgccccg cacgaccagc ctcaaaggaa gtccgcagaa gaagaaaagc
     5401 tcgtgaaact cctgcaacaa tccgcccgcg cgcagaggtg gaatattcag tacgcgtatc
     5461 gactcgctca ggaatttcag cgtctgcttc gggctattcg ggagcaagcc aggcgggaaa
     5521 cagacgaggc atttaaaaag gaacaggcgc gatataaaga tgccaccgat ggcgtctggc
     5581 tggacgaacg tgagcagtac tacaaggcgc tggccgaaag tctttacctc caacggcgcg
     5641 ccggattgat cgagacatta tttgcctggt ggtctgacgt ccttcgcgcg cgtaacaatg
     5701 tagagcacca taatttgccc gacgcaaagg aggagaccag cgtgctcgcg aagcggttca
     5761 gcacgccgga aattttgcaa cgcgtccgtc gtctcgaaga attgcgcgat catcttggtc
     5821 gcaatatcca ggaagcgctc gccatcgaag tagctttcct ggccatcttc actggctaaa
     5881 agcgggtagg gcgagactca gtcgagccga cgaaatcagg agcgggcttc gtttttgatc
     5941 cgcattagtg gattcggctt ggctcaccac agcgtctgcg taatctgcgg agatccaatg
     6001 agattcgaaa accagattgc gatcgtgacc ggagcggggc gggggattgg gcgtgcaatt
     6061 gctgttcacc tggccagcga aggcgcgcgt gtagcttgtg tgagccggac ggaagagaac
     6121 gcaaaaagga cggctgatga aatcaacatc atccgcgccg atgcggcgaa ggcgtacgcc
     6181 gtggatgtgg ccgatcacgc tgcggtgcaa aagatcggcg cgcagattct caaagatttc
     6241 atgaagatcg acatcctcgt taacaacgcc ggggtgaccc gtgatggcct ggcgatgcgg
     6301 atgtcgattg aagattggga cgcggtcgtc aacacgaact tgcgcggcgc gttcaatttc
     6361 acgcaggcga tcgtgcgttc catgaccaaa cagcgaagcg gccggatcat caatattagt
     6421 tcggtaatcg gattgatcgg caacgcgggc caaaccaatt acgccgcgag caaagccggc
     6481 ttgatcggtt ttaccaaatc actcgcgaaa gaattagcca gccgcaacat caccgtgaac
     6541 gccgtcgcgc cgggatttat tacgacagac atgaccgcgg acctttcgga cgaaatcaag
     6601 aaatccattc actcgaaaat tccgcttggc agaaccggcg ggcccgaaga aatcgccagc
     6661 gctgtggcgt ttcttggatc ggccgaggca aattacatca caggccaggt gctttgcgtg
     6721 gacggcggga tagtgatgta gcgctttctg ta
//
LOCUS       DASQUQ010000042         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1087839, whole genome shotgun sequence.
ACCESSION   DASQUQ010000042 DASQUQ010000000
VERSION     DASQUQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087839"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..458)
                     /locus_tag="VE486_01190"
     CDS             complement(21..458)
                     /locus_tag="VE486_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37733.1"
                     /translation="MRFAKSSCLIATVFLLATAANVPAAVVKANGFQLKLNGQPFVIK
                     GMNYSPVPIGTAPGIIPYGDYFVPAYANVWKPDIDKMREAGINVIKLYAGNPDLNAGL
                     PGTAGHWKQFLDYCYNGGRKPIYVVMFSYTQGRRDRARRARLL"
     gene            640..2364
                     /gene="ccsA"
                     /locus_tag="VE486_01195"
     CDS             640..2364
                     /gene="ccsA"
                     /locus_tag="VE486_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZA37734.1"
                     /translation="MKTLIFILVALGSVFFRDAGCGQSNLPPANSSSIDFKQFGLLAI
                     QDGGRRKPIDTFARETLIRITGRSTYADNAGRKWQPNDFVLSALLETHDWKNEPMVLI
                     SSGQLIEQLGLDKTQRRFSFAQLTGSAELQRIANEARALKRAEKPLDRIQQEALAVSD
                     RLALLGNVMNGRALLIVPSPKNEADAWAVPDPEAVAIYYNETQVAPAFSELTKMMRAY
                     AQGDAFNFGSAARQLRDNLRALSPSIYPQERQLRLEYFYNHFAGFYRAIWCYGIALVI
                     LVVAHLRKRGRALQNLGVVIALVGLAFQAAGITMRCMIAGRPPVTNMYESIIWVSFAV
                     LFFGMIFFARYRTPVYLLAALPVTLVALLLVHQMPIAMPSSIDPLVPVLRDNFWLTVH
                     VLTITLSYAAFALAMGFGHILLWRYARNPAATSADAPMHFWLYRVLQLGVLLLAAGTI
                     LGGVWANYSWGRFWGWDPKETWALIALLCYILTLHGRLAGWWTEFGLVVASVVCFLAI
                     LMAWYGVNFVLGKGLHSYGFGIGGETYVAIFVVLDFLFVAFAIWRYRRSKRLVSPAGE
                     ITGERVAV"
     gene            complement(2761..3261)
                     /locus_tag="VE486_01200"
     CDS             complement(2761..3261)
                     /locus_tag="VE486_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZA37735.1"
                     /translation="MFARIIQQISLPLMAIALADLLDEKQVILRLRSRKPGNALRAIV
                     QLLAENEKIDQPEKFLEQVLAREQAHPSVVEHGVVFPHARTDLVDKIILGIGRSRAGI
                     PFGQNGVRAHLIFVIGVPQQLVNDYLICVGTLARLMKDDAIRSTLLHAETPREFLDAL
                     TPETTP"
     gene            3286..4005
                     /locus_tag="VE486_01205"
     CDS             3286..4005
                     /locus_tag="VE486_01205"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HZA37736.1"
                     /translation="MATEKIPAGSNEEATVSLQRSIYDPGYVNAMSHFYRGEMGRIMV
                     WRQRLDITTNWAITSSTAIITIAFTTREVPHIIFFFNLAIVWAMLWIEARRYRFYDAF
                     RARVRMLEAHFLVPMVMENREMLQGEWKKLVCEDLILPCFKISKLEAVGRRLKRNYVF
                     IFILIMIAWVTKIFLHASAPIEGPRSFYHALRIGHIPSWLVAFIFAGTLVSVTAITIY
                     VSRKSSGEISEFGTHRSLWRI"
     gene            4058..>4410
                     /locus_tag="VE486_01210"
     CDS             4058..>4410
                     /locus_tag="VE486_01210"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HZA37737.1"
                     /translation="MSDHRSPITDHFAKLCEIVAKLRAPGGCPWDREQTHESLLPALI
                     EEAYETAGALRAKDDANFREELGDLLLLIVMHAEIAREGGRFNIDNVLADVTEKLIRR
                     HPHVFGKSDARDSGAV"
BASE COUNT          929 a         1167 c         1287 g         1027 t
ORIGIN      
        1 catcgtactg cctgatgtaa tcaaagaagc ctggcccgcc ttgcgcgatc acgccgccct
       61 tgcgtgtagg aaaacatgac cacgtaaatg ggtttgcgtc cgccgttgta acaataatcg
      121 aggaattgtt tccaatggcc agcggtgccg ggcaggcccg cgtttaggtc gggattaccg
      181 gcatacaatt tgatgacgtt aatgcccgct tcgcgcattt tgtcgatgtc cggtttccag
      241 acgttggcat aagcgggaac aaagtaatcc ccataaggaa taattcccgg ggccgtgccg
      301 atcggtaccg gcgagtaatt catgcctttg atgacaaagg gctgtccgtt gagctttagt
      361 tgaaagccgt tggccttcac tacagcggca gggacgtttg cggctgtggc gagcaggaaa
      421 actgtggcga taaggcaact ggatttcgca aagcgcatcc ccgtttttgc aattgaaaga
      481 aggaacataa gtggcgcctc gtatgctggg tttgcgcttg gctgtcaagc attattctca
      541 gcaatttttt tataaattat aaatatagaa taaatataga acgccaagtt ttgatgcttt
      601 gctcgacatg acgcacgtcg agaattagat tcgcgccgct tgaaaacgct catcttcatt
      661 ctcgtcgcgc tgggcagcgt gttttttaga gacgcaggtt gcggccaatc taatctgccg
      721 cctgcgaata gcagcagcat tgatttcaaa caatttggac tgctcgcgat tcaggatggc
      781 ggaaggcgta aaccaatcga cacgttcgcg cgagaaacgc tgatccggat caccgggcgg
      841 tccacatatg ccgataacgc aggccggaaa tggcagccga acgatttcgt tttgtcggcg
      901 ctgctcgaga cgcacgattg gaagaacgaa ccaatggtgc tgatttcatc aggccaatta
      961 atcgagcagc ttggactcga taaaacacaa cggcgattct cgtttgcgca attaaccggg
     1021 tccgcggaat tgcagcggat cgcgaacgag gcacgcgcgc tcaagcgcgc ggaaaaaccg
     1081 ctcgaccgaa ttcagcagga agccctcgcc gtcagtgacc gattggcatt actcgggaac
     1141 gtgatgaacg gaagggcgtt gctcatcgtt ccgtcgccga aaaatgaagc ggatgcgtgg
     1201 gcggtgcccg atccagaggc ggttgcgatt tattacaacg aaacgcaggt cgctccggct
     1261 ttctctgaac tgacaaaaat gatgcgcgct tacgcgcaag gagacgcatt caatttcggc
     1321 tccgctgcgc gtcagttgcg cgacaacctg cgagcgctca gcccgtcgat ttatccgcag
     1381 gagcgacaat tgcgcctgga atatttctac aatcatttcg cagggtttta tcgcgcgatc
     1441 tggtgctacg ggatcgcgct cgtgattctg gtggtcgcgc acctgcggaa acgcggccgc
     1501 gcgttgcaaa atcttggcgt cgtgattgcg ctcgttggtc tcgcgtttca agcggccggg
     1561 atcacgatgc gttgcatgat tgccggacgt cctcccgtca caaacatgta tgaatcgatc
     1621 atctgggtgt cattcgcggt attgtttttc gggatgatct tttttgcgcg ttaccggacg
     1681 ccagtttatc ttctcgcggc gttgccggta acgctggttg cgctgttgct cgtgcatcaa
     1741 atgccgatcg cgatgccgtc gagcattgat ccgcttgtgc cggtattgcg ggataatttc
     1801 tggcttaccg ttcatgtgct taccatcacg ctgagttatg cggccttcgc gctggccatg
     1861 gggttcgggc acattctgct ctggcggtat gcgcgcaatc cggccgccac gagcgcggac
     1921 gcgccgatgc atttctggct ctaccgcgtg cttcagctcg gcgtgctttt actggcagcg
     1981 ggcacgattc tgggcggggt ctgggcaaat tattcctggg gacggttctg gggctgggat
     2041 cccaaggaaa cgtgggcgtt gattgcgctg ctttgttata ttctgacgct ccatggccgg
     2101 ctcgccggtt ggtggacaga gttcggcctc gtcgtggcga gcgtggtttg tttcctcgcg
     2161 atcctgatgg cgtggtatgg cgtgaatttt gtgctcggga agggtttgca cagttacggg
     2221 ttcggcatcg gcggcgagac ttatgtcgcg atatttgtcg ttctcgattt tttgttcgtc
     2281 gcgttcgcga tctggcgata tcgaaggagc aaacgcctgg ttagtccagc cggcgaaatt
     2341 acaggcgaac gcgtcgctgt ttagcgccgt agccgaggag cattctgccg gacggctcgc
     2401 gtggaacccg gttagcgtag agattcggta ccatgtccaa atgaagacgt ctgcgggttc
     2461 gcggcagaat gccacgaccg acgggctaaa agcccgcgct ccccaagcga tgcgatcttt
     2521 ttcgtagcgt catacgaatt taaagtatgg caggacgcag aacgaaatac cggcggcgac
     2581 cgctccgccc gcaggcgcct ccagcacgac ccggcgaaaa tattgagacg ggcggcacga
     2641 aaatcggcgt caagagcggc gcgcaaaaat ctgagcgtac ccgggcaaag cgtcgctcgg
     2701 ccggcgtgcg ctgattctgt agccggcctc ggtataccgg cccgcgatca ccgattgccg
     2761 ctacggagtt gtctccggtg tgagcgcatc cagaaattcg cgtggcgttt cggcgtggag
     2821 cagtgtcgag cgtatcgcat catctttcat cagtcgtgcc agcgtcccaa cgcagatcag
     2881 gtaatcgttg accaactgtt gcggcacgcc gatcacgaaa atcaggtggg cgcgcactcc
     2941 gttttggcca aagggaatgc cggcgcgact gcgcccaatg ccaagaatga ttttgtcgac
     3001 caaatcggtg cgcgcatgcg gaaagacaac gccgtgttcc acgacgctgg gatgcgcctg
     3061 ctcgcgcgcg aggacttgtt cgagaaattt ctccggctga tcgatcttct cgttttcggc
     3121 aagtaattga acgatcgcgc gcagcgcgtt ccccggtttt cgggagcgca atcgcaggat
     3181 cacctgcttt tcatcgagaa gatcagccag cgcgatagcc attagcggca acgagatttg
     3241 ttggattatc cgggcaaaca cagttatcca gttaatcgga tagccgtggc aactgaaaag
     3301 ataccagccg gatcgaacga agaagcaacg gtctcgttgc aacggagcat ttacgatccg
     3361 ggatacgtca atgcgatgtc acatttttat cgtggcgaga tgggacggat catggtctgg
     3421 cggcagcggc tcgacatcac cacgaactgg gcgattacca gcagcaccgc catcatcacc
     3481 atcgccttta ccactcgcga agtgccgcat atcattttct ttttcaatct ggccattgtc
     3541 tgggcgatgc tttggatcga ggcgcgccgt taccgtttct acgacgcatt tcgggcgcgc
     3601 gtccggatgc tggaagcgca tttcctggtc ccgatggtga tggaaaatcg cgagatgctg
     3661 cagggcgaat ggaaaaagct ggtctgcgaa gatttgattt tgccctgctt taaaatcagc
     3721 aaactggaag cagtcgggcg gcgattgaaa cgcaactacg ttttcatttt tattctcatc
     3781 atgatcgcgt gggtgacgaa aatattcctg cacgcgagcg cgccaatcga ggggccgcgc
     3841 tcattttacc acgccctgcg catcggacac attccttcct ggctggttgc cttcattttt
     3901 gctggcaccc tggtcagcgt gaccgccatc acaatttatg tcagcaggaa gtcctccggc
     3961 gaaatctcag agttcggcac gcatcgctcg ctctggcgga tctgatccag ttttaagttt
     4021 caagtgttaa gttttaagca aaccgaatca tggcgtgatg tctgatcacc gttcaccgat
     4081 cactgatcac tttgcgaagc tttgcgagat tgtcgcgaag ctgcgcgcgc cgggcggttg
     4141 cccgtgggac cgcgagcaaa cgcacgaatc gctgctgcct gcgttgatcg aagaggccta
     4201 cgaaacagcc ggggcactgc gcgccaaaga cgacgcaaat tttcgcgaag aactcggcga
     4261 tcttctgttg ctcattgtga tgcacgccga aatcgcgcgg gaaggcggcc ggttcaatat
     4321 cgacaatgtg ctggctgacg tgaccgagaa actcatccgg cgccacccgc atgtgttcgg
     4381 caaaagcgat gcacgcgata gcggcgcggt
//
LOCUS       DASQUQ010000043         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_550167, whole genome shotgun sequence.
ACCESSION   DASQUQ010000043 DASQUQ010000000
VERSION     DASQUQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550167"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VE486_01215"
     CDS             complement(<1..719)
                     /locus_tag="VE486_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZA37738.1"
                     /translation="MVTSKGVGLAVPDWPTTFGYNMFLFPVSKWVGGILFEHTHRLIA
                     SAVGFLTIILAIWLWRSEDRNWVRTLGVIALGLVILQGVLGGLRVTMLKDEIGIFHAC
                     LAQGFLGLLVVIALVTTKFWRSLSNVVIAPEKIGSITTLAIAITLAIYAQLALGATMR
                     HQHRDLAILDFPTANGAWIPDTSTAAIAKINASRDARGLSDVDAFQIWLQMAHRFLAL
                     IITVAVIAFCLRVLRDAPHIIA"
     gene            complement(907..1767)
                     /locus_tag="VE486_01220"
     CDS             complement(907..1767)
                     /locus_tag="VE486_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZA37739.1"
                     /translation="MSSLALINEFLGIPPNASSHGYQIDHIIEFCHWFMGALFLGWSA
                     FFIFVLIRFRKRRHPTADHHGVRSGISTHLEFSVVLIEAVLLLGFAIPLWAKRVNEFP
                     PSKDAILVNVVSQQFSFNFHLPGPDGQFGRRDVSFESNSNPIGLDPNDPAGKDDIATL
                     GELHVPVNRPVIIELSSKDVIHDFFLPHMRIAADAIPGMRIPMWFTPIKTGTYEVICG
                     QLCGLGHYSMRGTLVVDTPEEYQAWLKERAELAGGGAPQPAGAPKTENAPAKQENKPP
                     EPAASPTPSG"
     gene            complement(1760..2020)
                     /locus_tag="VE486_01225"
     CDS             complement(1760..2020)
                     /locus_tag="VE486_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37740.1"
                     /translation="MKAKILSILLLSIFSPVGQALACAFCYGAKDGKSTEHMAVAIWF
                     LFGAVMSVLGGIGAFSFHLWRQGRMPADPYQEIAEEDLSKYE"
     gene            complement(2017..2226)
                     /locus_tag="VE486_01230"
     CDS             complement(2017..2226)
                     /locus_tag="VE486_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37741.1"
                     /translation="MSLRGFHIVFIIFSTLLALGIGAWCIWVNLVEGAPIYLAGAIFS
                     FVAAVALIVYGVWFYRKMKRLRIIT"
     gene            complement(2227..2571)
                     /locus_tag="VE486_01235"
     CDS             complement(2227..2571)
                     /locus_tag="VE486_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZA37742.1"
                     /translation="MNNPPDVSQDPKEYEEYAHNVQKHVRGYLMVGATLLVFTAITVA
                     LSYVNFGASKANIAVAMLVATFKAGLVAAIFMHLAAEKRLIYRILIFTGFFVLGLFWL
                     TLLAWYDYPIVR"
     gene            complement(2575..>3153)
                     /locus_tag="VE486_01240"
     CDS             complement(2575..>3153)
                     /locus_tag="VE486_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZA37743.1"
                     /translation="LCGVIFLTVKLVYEWPQKFDHFGAYIKPEALGKYEQYLGNEHAA
                     AKGLPPRLEITGHLHNRAALNDPKIKEYEVALDPLNAEPTNPAMDRPHFFYLPPTHKI
                     ATIEKEDVQYATMFVPKHSAYFASYFTITGLHGLHVLGGVLVFIYMWLPVSKRLYQRN
                     PEHLANRVEVSGLFWHFVDLIWIFVFPLFYLL"
BASE COUNT          818 a          859 c          858 g          618 t
ORIGIN      
        1 gcaatgatat gcggcgcgtc tctcaagacg cgcaggcaaa acgcaataac agcaacagta
       61 ataatgagcg caagaaatcg atgcgccatt tgcagccaga tttggaacgc gtccacgtcg
      121 gataggccgc gggcatcgcg cgacgcattg atctttgcaa ttgccgcggt gctcgtatcc
      181 ggaatccagg cgccgtttgc ggtgggaaaa tcgagaatcg ccagatcccg atgttgatga
      241 cgcatcgtcg ccccgagcgc aagctgcgca taaatcgcca gggttattgc aatggccagg
      301 gttgtgatcg atccaatctt ttctggagcg atcacgacat tcgagagcga ccgccaaaat
      361 tttgtcgtca ccaacgcaat cacgacaagc agaccgagaa acccttgcgc gagacaggcg
      421 tgaaagatcc cgatctcatc tttcagcatc gtgacgcgga gcccgccaag aacgccttgg
      481 agaatcacca aaccgagcgc gatgactcca agagtgcgaa cccagtttcg atcttcgctg
      541 cgccaaagcc agatcgcgag gatgatcgtc aggaatccaa ctgctgaggc gattaagcga
      601 tgcgtgtgct caaacaaaat tccgccgacc cattttgaga cgggaaataa aaacatgttg
      661 taaccgaacg tggtcggcca atcgggcaca gcgagaccga cgcctttgct ggtcaccatt
      721 ccgccgctgc aaatgagaaa cagggtcgcg atgcacgtaa gccatgcaaa acggttgagc
      781 caagagctgt agcggcggtc tgtgaccgcc gcacttttag atgtcggtcc acgcacggtc
      841 agaataatag acccgacgag acaaatcgga caaatgcgac ggtcgcagac tgccgctaca
      901 gaaagactac ccgcttggag tcggcgaggc tgcgggctcg ggcggcttgt tctcttgctt
      961 cgcgggggcg ttttccgttt tcggcgcacc ggctggctga ggggcgccgc cgccggccaa
     1021 ctccgcgcgt tctttgagcc acgcctgata ctcctctggc gtatcaacca ccagcgtccc
     1081 tctcatggaa taatggccca aaccgcatag ctggccgcaa atcacttcat aagtcccggt
     1141 cttgatggga gtgaaccaca tcgggatgcg cataccaggg attgcgtccg ctgcaatccg
     1201 catgtgcggc aggaaaaaat cgtggatcac gtctttcgat gaaagctcaa tgataacagg
     1261 gcgattcacc gggacatgca gttcgcccag cgtcgcgata tcgtctttgc cggccggatc
     1321 gttgggatcg agaccgatgg gatttgagtt gctctcaaag ctgacatcgc gccggccaaa
     1381 ttgcccgtct ggcccaggca agtgaaaatt aaagctgaac tgttgcgaga cgacattaac
     1441 aagaatcgcg tccttgctgg gtggaaattc attcacgcgt tttgcccaaa gcgggatcgc
     1501 gaagccgagc aggagcaccg cttcgatcag gacaaccgaa aattccaaat gtgtcgaaat
     1561 gccgcttctc acgccatgat gatcagcagt cggatgccgc cgtttccgga agcgaatcaa
     1621 aacgaaaata aaaaacgccg accaaccaag aaagagcgca cccatgaacc agtggcagaa
     1681 ctcgatgatg tgatcgattt gatatccgtg cgaagaggcg ttcggcggta ttcccaaaaa
     1741 ttcattgatc agcgcgagac tactcatatt tgctcaggtc ttcctcggcg atttcctggt
     1801 aagggtcggc cggcatgcgt ccctgccgcc agaggtgaaa actgaatgcg ccgattccac
     1861 ccagcaccga catgacggcg ccaaaaagga accagatcgc caccgccatg tgctcggtcg
     1921 atttcccatc ttttgcgccg tagcagaacg cgcacgccag cgcctgacca actggcgaaa
     1981 agatcgacaa gagcagaatc gacaagattt tcgctttcat gtgatgatgc gaagccgttt
     2041 cattttccga tagaaccaaa cgccgtaaac aatcagcgca accgcggcca caaacgagaa
     2101 gatcgctccg gcaagataaa tgggcgcgcc ttccaccagg ttgacccaga tgcaccacgc
     2161 tccaatcccg agcgcaagca gcgtcgagaa aataataaaa acaatatgga agcctctaag
     2221 tgacatttaa cgaacgatcg gatagtcgta ccacgccaac aatgtaagcc agaaaagtcc
     2281 gagcacaaaa aagccggtga aaatgaggat gcgataaatg agccgctttt ccgcggcgag
     2341 atgcatgaaa attgccgcca ccaaccctgc cttgaacgtc gccaccagca tggccacggc
     2401 gatgttcgct ttcgacgccc cgaagttcac gtaggaaagc gcgacggtaa tagcggtgaa
     2461 cacgagcaac gtggcgccga ccatcaaata acctcggacg tgcttttgca cgttgtgcgc
     2521 gtactcctcg tattccttcg gatcttggga aacatcaggc ggattgttca tggcttagag
     2581 cagataaaaa agtggaaaca cgaaaatcca gattagatcg acaaagtgcc agaagagacc
     2641 agaaacttcg acgcggttgg ccagatgttc ggggttgcgc tggtaaagcc gtttgctgac
     2701 cggcagccac atgtaaataa acacgagcac accgccgagc acgtgcagcc cgtgcaaacc
     2761 agtgatggtg aaatagctgg caaaatacgc gctgtgtttg ggcacaaaca tcgtcgcgta
     2821 ttgcacgtcc tccttttcga tcgtggcgat cttatgggta ggcggcagat agaaaaagtg
     2881 cggtcgatcc atcgccggat tggtcggctc ggcattcagg ggatcgagcg cgacttcata
     2941 ttccttgatc ttgggatcgt ttagagcggc gcgattatgc aagtggccgg taatctcgag
     3001 ccgcgggggc agacctttcg cggcagcatg ctcgttgccg agatactgtt cgtacttccc
     3061 gagggcttcc ggcttaatgt aagcgccgaa gtgatcaaac ttctgtggcc attcgtaaac
     3121 gagtttgacg gtcaagaaaa tcacgccgca aag
//
LOCUS       DASQUQ010000044         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1275373, whole genome shotgun sequence.
ACCESSION   DASQUQ010000044 DASQUQ010000000
VERSION     DASQUQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275373"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2058)
                     /gene="pbpC"
                     /locus_tag="VE486_01245"
     CDS             complement(<1..2058)
                     /gene="pbpC"
                     /locus_tag="VE486_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HZA37744.1"
                     /translation="MMGRLYPGSARVLACKRQSGSDFRRRAETGLPLNVNRRAEASAQ
                     EKFAIAGRARQTRETRALPKALRLLSFVICAVVVLAMAMVWWLPIPDALQKSPAGTLT
                     LLDCRGRELAEIASPEARAQLPRPLDEMGKWLPRVAVALEDHRFYEHAGVDWRATASA
                     CTRNLRAHRIVSGASTITQQLVKIASGRQRRSWFGKVYETIVAWKIERRWSKERILTE
                     YLNRSSYGNRRLGPEAAARAYFGKSARDLTLAESIFLAGLPQAPTRFNPWRRPELATR
                     KYERSVARLAQLGVISGEQQVLLAKSPPVAHRFDPPHLATHFVDAVIARNPSLRGTVR
                     TSLDLDLQNTAERLVRSHLGALNRHDITQAAIVILDNETGAVRAMVGSNNYATSQVNG
                     AMRPRSCGSTLKPFVYLAAIDRHLLTAASVLPDTAEAIRDEYADYDPQNYNHRYLGPV
                     RLREALGCSLNVPAVFALSQLGARGAFFELQKWGFKFPRALSDYGAGFVLGNAEIRLV
                     DLAGAYAGLARNGISMQAKFLTAEHHPMTRIASGEPTAIITDILSDNDARQKSFGAHS
                     PLAFEERIAAKTGTSSGFRDAWTVGFDKEHTVAVWAGNFDGRPMRDTFAVRSATPLWA
                     AMMRELLRRDRPLDPPKESERLVRREICADTGLLASRLSASRISELFLKGTEPKEDSA
                     NWFS"
     gene            complement(2055..>3015)
                     /locus_tag="VE486_01250"
     CDS             complement(2055..>3015)
                     /locus_tag="VE486_01250"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37745.1"
                     /translation="NESGDEGRAILAMALHRQNIMAHEKEQLLKEIDAPIKERAFNPA
                     TLGSMTRAEAIWALAFDTIAPKFYTAQKKQRIRERMLALMDSSAALSTQENLWLLLAF
                     KSMIGTEKAEALSAAEPKGVGSKNGRSAAWLDRKLDNQLLVKGLNKTALSFLMQAEYS
                     TVEVDTDRVDRGFRVERVVRNLTESKRTGAINTPFKLGDQILVTYRINTRKKQNYVAL
                     EDSLPAGLETVNPNLATIGKFFELPASDQPERALALSYSEMRDRSTLLYFDDFEPGSG
                     TYSVLARATAAGTFRWPATQVVPMYDSRFSGLSPSSVCAISGE"
BASE COUNT          610 a          897 c          872 g          636 t
ORIGIN      
        1 gctgaaccag ttcgcggagt cttccttcgg ctcggttcct tttaaaaaca gctcgcttat
       61 tctcgaggcg ctgagccgcg acgccagcaa tcccgtgtcc gcgcagattt cgcgccggac
      121 gagccgctcg ctttccttcg gaggatcgag cgggcgatcg cgccgcagca attcgcgcat
      181 catcgccgcc caaagcggtg tggccgaacg aacggcaaag gtgtcgcgca tcggccggcc
      241 atcaaaattt cccgcccaaa ccgcgacagt gtgctcttta tcgaagccga ccgtccatgc
      301 atcgcgaaag ccgctgctcg ttccggtttt tgcggcgatc cgttcttcaa atgcgagtgg
      361 cgaatgcgca ccgaaacttt tctgcctcgc atcgttatcg gacaagatgt cggtaatgat
      421 cgccgtcggt tcgccagagg cgatccgcgt catgggatga tgctcggcgg tgagaaattt
      481 cgcctgcatg gagatgccgt tccgcgccaa accggcatac gctccggcca gatcgaccag
      541 acgaatctcg gcattgccca ggacgaaacc cgcgccgtag tcgctcaatg ctcgtggaaa
      601 tttgaacccc cacttttgta actcaaaaaa agctccgcgc gcgccgagct ggctcagggc
      661 aaaaactgcg ggaacattga gcgagcaccc gagcgcctcg cgcaagcgca ccggaccgag
      721 gtaacgatga ttgtaatttt gcggatcgta atccgcgtac tcgtcgcgaa tggcctcggc
      781 ggtgtcaggc aatacgctgg cagcggttag aagatgtcga tcgatcgcgg cgagataaac
      841 gaacggtttt aaggtggagc cgcaactacg cgggcgcatc gcgccgttga cctggcttgt
      901 cgcgtagttg ttcgaaccga ccatcgcccg caccgcgccg gtttcgttat cgagaataac
      961 gattgcagcc tgcgtgatgt catggcgatt cagtgcacca agatgggagc ggaccaatcg
     1021 ttctgctgta ttttgcagat cgagatcgag cgatgttctc accgtgccgc gcagcgacgg
     1081 attccgcgcg atcaccgcat cgacaaagtg cgttgccaga tgcggtggat cgaaccggtg
     1141 cgcaacgggc ggagatttcg ccagaagaac ctgctgctcg ccactgatca cacccaactg
     1201 cgccagacgc gcgaccgagc gttcatattt ccgagtcgca agctcgggac gccgccaggg
     1261 attgaatcga gtcggagctt gcggcagacc ggcaaggaaa atcgattcgg ccagcgtcag
     1321 gtcccgggcg gatttgccaa agtaagcacg cgcagccgct tccggcccga gccggcgatt
     1381 gccgtaactg cttcgattca aatattcggt gaggatgcgt tctttgctcc agcggcgctc
     1441 gattttccag gccacgattg tttcataaac cttgccaaac cagctgcgcc gttgccggcc
     1501 cgaagcgatc ttcaccagtt gttgggtaat ggttgatgcg cccgaaacga tccgatgcgc
     1561 tcttagattt cgtgtgcaag ccgatgccgt cgcccgccag tcgacgccgg cgtgttcgta
     1621 aaagcgatga tcttcgagag ctaccgccac gcgcggaagc cactttccca tttcgtcgag
     1681 aggacgcggc aattgcgcgc gcgcttcagg cgaagcgatc tcggccagct cgcggccccg
     1741 acaatcgagg agtgtcagcg ttccagccgg cgatttttgt agcgcgtcag ggattggcag
     1801 ccaccaaacc attgccatcg ccaagacaac aacggcgcaa attacaaatg agagcaaacg
     1861 caaagctttg ggtagcgcac gcgtctcgcg tgtctggcga gcgcgtcccg cgatcgcgaa
     1921 cttttcctgt gcgctcgcct ccgccctgcg atttacattt aaaggcaagc ctgtttcggc
     1981 gcgacgccga aagtccgagc cggactggcg tttgcacgcg aggacgcgtg cgctacccgg
     2041 atacaaacgg cccatcattc gccggaaatt gcgcatacac ttgagggcga taatcccgag
     2101 aaacggctat cgtacatcgg gacgacttgt gtggccggcc agcgaaacgt gccggccgcc
     2161 gtcgcgcgcg ccagcaccga atacgtgccc gaccccggtt caaaatcgtc gaagtaaagc
     2221 agtgtggaac gatcgcgcat ttccgagtac gaaagggcca gcgctcgctc gggttgatcg
     2281 ctcgccggga gttcgaagaa cttcccgatc gtggcgagat ttgggttcac cgtctcaaga
     2341 ccggccggaa gcgaatcttc gagcgcgacg taattttgct tctttcgcgt attgatccgg
     2401 taggtcacga gaatctggtc cccgagtttg aatggcgtgt ttatcgcacc tgtgcgtttg
     2461 ctttcggtga gattgcgcac gacacgctcg acgcgaaatc cgcggtcgac gcgatccgtg
     2521 tcgacttcca cggtagaata ttctgcctgc atcaaaaacg acagcgctgt tttgttcaga
     2581 cctttcacca gcagctgatt atcgagcttg cgatcaagcc aggctgcgga gcgaccgttt
     2641 ttcgagccaa cgccttttgg ctcggccgca ctcagcgcct ccgctttctc cgtgccgatc
     2701 atggacttga atgcgagcag caaccaaaga ttttcctggg tcgaaagtgc ggccgaggaa
     2761 tccatcagcg ccagcatgcg ttcgcggatg cgttgtttct tttgcgcggt ataaaatttt
     2821 ggcgcaatcg tgtcgaatgc gagcgcccaa attgcctcgg cccgcgtcat tgaaccaagc
     2881 gttgctggat taaacgcgcg ttccttgatc ggcgcgtcga tttctttgag caattgctcc
     2941 ttttcatgcg ccatgatgtt ttgccggtgg agcgccatcg ccaggatcgc gcgtccttca
     3001 tcgccgctct cattg
//
LOCUS       DASQUQ010000045         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1178486, whole genome shotgun sequence.
ACCESSION   DASQUQ010000045 DASQUQ010000000
VERSION     DASQUQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178486"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VE486_01255"
     CDS             complement(<1..221)
                     /locus_tag="VE486_01255"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HZA37746.1"
                     /translation="MKRTLILAVLFGVAISLFGGLSRGQNSPPSATKTAILAGGCFWC
                     IQPAFDRAKGVLKTVVGYSGGTEPNPTYEL"
     gene            315..896
                     /gene="trmB"
                     /locus_tag="VE486_01260"
     CDS             315..896
                     /gene="trmB"
                     /locus_tag="VE486_01260"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HZA37747.1"
                     /translation="MVKLIPESYVAALDLEKTFGRLAPLHVDLGCGDGSFISALAEKM
                     PEKNFLGIERLLGRIRSAERKAATVCNVRLLRMESSYAVRYLLPAGSVEIFYLLFPDP
                     WPKRRHWRRRIVTAEFLRAISDALVGNGRLLIATDHLDYFEKIKEIARSNPDFTVAPP
                     GAADLPVTKFEQQFRAQGAAIHWLELRKVSPVR"
     gene            complement(857..3157)
                     /locus_tag="VE486_01265"
     CDS             complement(857..3157)
                     /locus_tag="VE486_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZA37748.1"
                     /translation="MKLVSAPRQPFVGLALMAGMGIILADFFPLPSSALVATAIIIVL
                     GAVAVLLWPRLLATYVLVGAGFFLLHGFQTSNTEGLKIAAQLGNRPRVVTATGSVISE
                     PKIAPSGFATFLLKLESIELEGRKQPTRAVWQVRWQGNPEFGDELKLFGKAEVIAPPR
                     NPGEFDMRAYLARRDVRRTLFARYPEDGTLIRHGGGNPILRMAQESRLWMQNALCRGL
                     EDPSEVRDFLSGIVLGLRHQTPEDIEEPFQQTGTLHLFSVSGLHVAIVAALLWMLATV
                     ARLPRKWATALIIPSLFFYAAVTGLHVSSMRAAVMASILLGGFFFDRKVFVLNSLAAA
                     AFFLLCWNTNELFSTGFQLSFAVVGAIILFAEPLFEFLQRWGAPDPFLPRSLLRGPHH
                     LMHAGFEWLCRGTSVSLAAWIGSLPFILWYFHIVTPISLFANLVVVPIAFFILAIAML
                     SLLATPLLAWVAVVLNNANWFLARVVLGIVQLFAQIPGGHFYVAAPHWPEKLTATITV
                     LDLGGGAAIHLRTEGANWLFDCGSERSYERVVREYLHWAGVNRLDGLFLTHGDSLHLG
                     GAAQLLHDFPRVRLIDNPAPDRSSVHRRLQRLFQERGIKPDNLAAGDRFRLSRGVTAH
                     ILFPPRIFSARRTDDQADVIQTSIAPSTSVLFMSDSGVETERALLAYGLNLHSDIVVK
                     GQHHSGQSGSGAFLDAVRPRLIIASSRDFPPNERIGDTWAEDLRTRGIKLFRQDETGA
                     VTLRFGRAGWEAQSYLTGETFRSSSQ"
BASE COUNT          821 a          926 c          961 g          678 t
ORIGIN      
        1 agttcgtaag ttggattcgg ttctgttccg ccagaataac ccacgacagt tttgagcacg
       61 ccttttgccc tgtcgaacgc gggctgaatg caccaaaaac agccgccagc gagaattgcg
      121 gtcttggttg ctgaaggagg cgaattttga ccgcgactaa gaccgccgaa aagtgaaatc
      181 gcgacgccaa acaacactgc aagtatgagg gttcttttca taatttcgat cttagacaaa
      241 gctgcgggcg atttgtttcg tctaaaccgc cgctccttgc ttgaaccatc gcgcggcggg
      301 cgcgatttgt ctgcgtggtc aaattgattc cggagtcgta cgttgcggcc ctcgatttag
      361 aaaaaacttt tggccgactc gcgccgctgc acgtcgatct cggctgcggt gacggctcgt
      421 tcatttccgc gctggcagag aagatgcccg agaaaaattt tctaggaatc gagcggttgc
      481 ttggtcgaat ccgcagtgcc gagcgaaagg ccgccacggt ttgcaatgtg cgcctgctgc
      541 ggatggaaag ttcgtatgcg gtgcgttatc tgttgccggc tgggtcggtg gaaatatttt
      601 atctcttgtt ccccgatcca tggccgaaac gccgtcattg gcggcgcagg atcgtgaccg
      661 cagaattcct gcgcgcgatt agcgatgcat tggtcggaaa tggaagactc ctgattgcga
      721 ccgatcacct tgattacttt gaaaaaatca aagagatcgc gcgatcaaat cccgatttta
      781 cggtcgcccc tcctggtgcc gcagatcttc cggttacgaa atttgagcaa caatttcgag
      841 cgcaaggcgc ggcgattcac tggctggagc tgcgaaaagt ttcgccagtg agataacttt
      901 gcgcctccca accggcgcga ccaaagcgca aggtaaccgc gccggtctca tcctggcgaa
      961 agagtttgat gccccgggtt cgcaaatcct cggcccaggt gtcgccgatg cgttcgttcg
     1021 gaggaaaatc gcgcgaactg gcgatgatca ggcgcggccg cacagcatcc agaaaggctc
     1081 ccgaaccaga ttgcccggaa tgatgctgtc ctttcacaac gatgtcgctg tggagattca
     1141 gaccataagc caggagcgcg cgctcggttt ctacaccact gtccgacatg aacaaaacag
     1201 acgtcgaagg agcgatggat gtttgaataa catccgcctg gtcgtctgtc cttctcgcag
     1261 aaaatattcg gggcggaaag agaatgtgcg cggtcactcc acgtgacaat cggaatctgt
     1321 ctcccgcagc caaattatcg ggcttgattc cgcgctcctg aaacaggcgt tgcaatcgcc
     1381 gatgaacact tgagcgatcg ggcgccggat tgtcgatcag ccggacgcga ggaaaatcgt
     1441 gcagcaattg cgccgcaccg ccgagatgga gcgaatcgcc gtgagtgaga aacaagccat
     1501 cgagccgatt tactcccgcc cagtgcaaat actcgcgaac cacgcgttcg tagctccgtt
     1561 cgcttccaca atcgaaaagc cagtttgcac cctctgtccg cagatgaata gccgcgcccc
     1621 cacctaaatc cagaacggtg atcgttgctg tgagtttttc aggccaatga ggcgccgcga
     1681 cataaaaatg tccgccagga atttgcgcga acagttgcac gatcccaaga acaacccgtg
     1741 ccagaaacca gttggcatta ttaagaacta cggcgaccca cgccagtaat ggcgtcgcga
     1801 ggagcgagag catcgcaatc gcgagaatga agaaggcaat cggaacgaca accaggttcg
     1861 cgaataatga aatgggcgta acgatgtgaa aataccagag aatgaatggc aacgaaccaa
     1921 tccaggccgc gagcgagacg gaggtcccac gacagagcca ttcgaaacca gcgtgcatga
     1981 gatgatgtgg gccacgtaat agactccgcg gtaaaaaagg atcgggcgcc ccccagcgct
     2041 gcaaaaattc gaagagaggc tccgcgaaca aaatgatcgc gcccacgact gcgaaggaga
     2101 gttggaaacc ggtcgaaaag agctcgttcg tattccagca gagaagaaaa aaagccgcgg
     2161 cagcgagact gttgagcacg aaaacttttc ggtcgaaaaa gaaaccgccc agtagaatcg
     2221 aagccatcac cgcggcacgc atgctcgaaa cgtgcaatcc ggttaccgct gcgtaaaaaa
     2281 agagtgacgg gataatgagc gcagtcgccc atttgcgcgg cagtcgcgcg actgttgcca
     2341 gcatccacaa aagtgccgcc acaattgcta cgtgcagacc agagacagag aacaggtgca
     2401 gcgtgccggt ttgttgaaat ggttcttcaa tgtcttcagg tgtttggtgc ctgagcccga
     2461 gcacgattcc gctgaggaaa tcccgcactt cggacgggtc ctccaacccc cggcaaagtg
     2521 cgttttgcat ccataaccgc gactcctgcg ccatgcgcaa gatggggttc ccaccgccat
     2581 gtcgaatgag cgtgccgtcc tctgggtagc gagcaaataa cgttcggcga acgtctcgac
     2641 gcgcgaggta ggcgcgcata tcgaattcac ctggatttcg cggaggcgcg atgacttccg
     2701 cctttccgaa aagcttcaat tcatcgccga attccggatt accctgccag cggacctgcc
     2761 agaccgcgcg tgtcggttgt ttcctgcctt cgagttcaat tgactcgagc tttagcagga
     2821 atgtcgcgaa cccgctcggt gcgatcttcg gctcgctgat cacggatccg gtcgctgtga
     2881 cgacccgcgg ccggttacca agttgagcgg cgatcttcag tccttcggta ttgctggttt
     2941 gaaagccgtg cagcaggaag aagccagccc caacgagtac gtaggttgcg agcaagcggg
     3001 gccaaagcag gacagcgacg gcgccgagca cgatgattat cgcagttgcc accagtgcgg
     3061 aagaagggag aggaaagaag tccgccagga tgattcccat gccggccatt agcgccagac
     3121 caacaaatgg ctgacgcggc gcactcacca gcttcatcga tgggcttttc gcttatcagg
     3181 ccttggcaag gcaagcttcc aatcgcgagc ggcggtcatc gaagccttta ggaaaaacgc
     3241 ccagcgccaa cacgcgaacg ctcatctatc tgaattggat gttgggcgtt cggcgttgag
     3301 cgttggaggt tgaatgcctg ttatgaagat ttggtacccg gcacgagcaa caccggacgc
     3361 gcactgtgtt tgagcacgcc tttggt
//
LOCUS       DASQUQ010000046         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_731409, whole genome shotgun sequence.
ACCESSION   DASQUQ010000046 DASQUQ010000000
VERSION     DASQUQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731409"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VE486_01270"
     CDS             <1..1097
                     /locus_tag="VE486_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37749.1"
                     /translation="HKPGVIYLGGNCVFRLTNRMEKFSVISPDLTRNEPGRTTAAGSG
                     AENYGTIFSLAESPKRAGLLWAGTDDGRLWITENDGGKWSELTNNLPEPARGQWVSRI
                     EPSHLDANVAYVATNAYRTGDDRPSILRTADLGKTWQSVTGDLPSADPVEVVREDPVN
                     PNLLYAGTHFGVFASFDQGAHWVRIGDVPSVRVDDIQIHPRTSDLVIATHGRSIAILD
                     DSVPFRDFTAEIAAKPTHLFSVRSVTARYLQPGFVEANGKGVYRGENPAEGALFTVWV
                     REFTGDEIKIAVTTATGQPVANLKAPGTPGFTRLNWDLRPTKDVRVEYGGEKPERFVA
                     AGDYTAELTFGSTKMKQSFHVDLAEGITPR"
     gene            1108..1719
                     /gene="coaE"
                     /locus_tag="VE486_01275"
     CDS             1108..1719
                     /gene="coaE"
                     /locus_tag="VE486_01275"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZA37750.1"
                     /translation="MPAIGITGGISTGKSTFVECLRELLPVATFFNADEAAHALLNRP
                     EVEKEIRYEFGAQVFSTAGDLNRANLRAIVFADAAKKRALERILHPRIRRQWRTEAKE
                     HRNSPKFFFADIPLLYETDGESLCDRVIVVACSQKVQLARLRKRMSVRSAEAKQMIKS
                     QMPLEEKIRRADHVVWNNGDRMSLMEQARFLVRSWQGQTWTKR"
     gene            1704..>2160
                     /locus_tag="VE486_01280"
     CDS             1704..>2160
                     /locus_tag="VE486_01280"
                     /inference="COORDINATES: protein motif:HMM:NF019138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37751.1"
                     /translation="MDEKVETSVSQTETVEAGVQPAHEQDAADTAATTAPRALDLNDL
                     QEFSEKKLKALARDLGLHLHPARSRHQHIVDIVRAAVAGGAIVSAEGFLDQVSDSFAM
                     LRWPKLNFLPLPEDVAVPRALIEQYDLRPGQKVSGTVRLPVQREKFLSLD"
BASE COUNT          492 a          553 c          657 g          458 t
ORIGIN      
        1 ggcataaacc gggcgtgatt tacttgggcg ggaactgcgt gttccggctg acgaatcgca
       61 tggaaaaatt ttcggtgatc agtcccgatc tgacgcgcaa cgaacccgga cgtacgactg
      121 ccgcggggag cggcgcagaa aattacggca cgatattttc gctggctgaa tcgccaaaac
      181 gtgcggggct gctatgggcg ggcacagatg acggccggct ttggatcacg gaaaatgacg
      241 gcggcaaatg gagcgaattg acgaataatt tgccggagcc ggcgcgcggc caatgggtgt
      301 caaggattga gccttctcat ctcgatgcca atgtggcgta cgtcgctacg aatgcttatc
      361 gcacgggtga tgatcgtccc tcgatattgc gtacggccga tctgggtaaa acgtggcaat
      421 cggtgacggg cgatctccca tcggccgacc cggtcgaggt ggtccgcgaa gacccggtca
      481 atccaaacct gctttacgcg ggcacgcatt tcggagtgtt cgcgtcgttt gatcagggcg
      541 cacactgggt gcgcattggc gatgtgcctt cggtccgcgt ggatgatata cagattcatc
      601 cgcgcacttc ggatctggtc attgcaacac acgggcggag cattgccatt cttgatgaca
      661 gcgtcccgtt tcgggatttc actgcggaga ttgccgcaaa gccgacccac ttgttcagtg
      721 tccgttccgt taccgccaga tatctacagc ctggatttgt agaagcgaac ggtaaaggcg
      781 tttatcgcgg tgaaaaccca gcggaaggcg cgttgttcac ggtttgggtg cgcgaattca
      841 ccggtgacga aatcaaaatc gctgttacca cagcaaccgg gcaaccggtg gcgaacctga
      901 aggcgccggg cacgccgggc tttactcgtt tgaattggga tttaaggcca actaaagacg
      961 tgcgggtaga atacggcggt gaaaagccgg agcggtttgt cgccgcaggt gattacaccg
     1021 ccgagctaac ctttggcagc acgaaaatga aacaaagttt ccacgtcgat ctcgccgaag
     1081 gcatcacgcc acggtgacgt tcgggtcatg cctgccatcg ggattaccgg cggaatttcc
     1141 acgggtaaaa gcacgttcgt cgaatgtctg cgcgagcttc tgccggtcgc tacattcttc
     1201 aatgcagacg aggcggctca tgcgctcctg aatcggccgg aagtcgaaaa agaaattcga
     1261 tatgaatttg gtgcgcaagt gttttcaact gcaggcgact tgaatcgggc gaaccttcga
     1321 gccatagtat ttgccgacgc ggccaaaaaa cgcgcgcttg agcggatcct tcatccgcgg
     1381 attcgccgcc aatggaggac agaggcgaaa gagcatcgca attctcccaa gtttttcttt
     1441 gctgacattc cacttcttta tgagaccgac ggcgaaagct tatgcgaccg agtgatcgtg
     1501 gtggcctgtt cgcagaaagt gcagctggcc cgtttacgaa aacggatgtc ggtcagaagc
     1561 gctgaagcta aacaaatgat taaatcgcaa atgccgcttg aggaaaaaat cagacgcgcg
     1621 gaccacgtcg tttggaacaa cggggatcga atgtcgctta tggagcaggc gcgatttttg
     1681 gtccgatcat ggcaagggca aacatggacg aaaaggtaga gacatcggtt tcacagacgg
     1741 aaactgtaga ggcaggggtg cagcctgcgc acgaacaaga tgcagccgac acggctgcga
     1801 ctacagcgcc gcgcgcactt gatttaaacg acctacagga gttttcggag aagaaactca
     1861 aagcgctggc gcgcgatttg ggtctgcacc tgcatcccgc ccgttcgcgg catcagcaca
     1921 ttgtcgacat tgtgcgggcg gcagtggccg gtggggcaat cgtttcggcg gaaggatttc
     1981 tcgatcaggt gagcgattcg ttcgctatgt tgcgctggcc gaagttgaat tttctgcctc
     2041 ttcccgagga tgtggccgtt ccgcgcgcac ttattgagca atacgatctg cggcccgggc
     2101 aaaaagtgtc ggggacggtg cgattgcccg tgcaacgcga gaagttcctg tcgctcgata
//
LOCUS       DASQUQ010000047         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1178514, whole genome shotgun sequence.
ACCESSION   DASQUQ010000047 DASQUQ010000000
VERSION     DASQUQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178514"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VE486_01285"
     CDS             complement(<1..651)
                     /locus_tag="VE486_01285"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZA37752.1"
                     /translation="MRVTIPGSTGCQPVASGSLPDASAAPRPVRPASFFSSETAMIRG
                     QRPRTAGWQPAFPGEIARDTISLVALVTLAIFCLTSCATPDTRHQIVISAREQKLALL
                     DRGNLMAVYPVSTSKFGLGDQPGSSFTPLGELEVAKKVGDHAPPGAVFKDRRRTGEIV
                     AIDSPGRDPIVTRILWLRGRETQNANAYARTIYIHGTPEERNIGLPASYGCIRMRST"
     gene            complement(663..1523)
                     /locus_tag="VE486_01290"
     CDS             complement(663..1523)
                     /locus_tag="VE486_01290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZA37753.1"
                     /translation="MLIETHAHLDYPEFAADLEDVLRRATEAGVTRIITIGTSIESSR
                     RAVGLAEKYPNIYAVIGVHPTNAEQAGEDVITPLRELAKSPRVVAIGETGLDYHHLPS
                     VEAAKQKNVQVFSALQSGTEEQVEASIEDGAYKSKQADLFEEQLDLAVELGLNVVIHQ
                     RDAWDDTLEIMRPYAGKVRGVFHCFGGTLEQANEVLDLDNLVSFTGVVTFKNGANVRD
                     VASKVPLWKFMVETDCPYLAPVPYRGKRCEPAHTLIVAETIAAARGISLEEIAEATSE
                     TAEKFFQFNR"
     gene            complement(1599..2621)
                     /locus_tag="VE486_01295"
     CDS             complement(1599..2621)
                     /locus_tag="VE486_01295"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZA37754.1"
                     /translation="MKRQPHSSPRSAKPLRTVSIIGTGSYVPEKILTNADLSRMVNTS
                     DDWITTRTGIKERRIAGKEENTSDMATKAALKAIQQAKISPKEINLILVATATPDMLF
                     PATACFVQKKIGATNAACLDISAACAGFLFGLEIAQQFITSHTHDTVLVIGAEKLTSI
                     TNWTDRNTCVLFGDGAGAAILRHRGSAHGVISTHIGSDGQYTDILFMPGGGSRCPITR
                     ENIDLNLQTIHMMGKEVYKQAVTAMLKAAKKALDQAGLSIEDIACVIPHQANVRIIEA
                     IADRLGISRDKMFVNLDRYGNTSAAAVAIALDEANRSGRIKPGDYVLMVVFGGGLTWA
                     STVLEW"
     gene            complement(2584..3615)
                     /gene="plsX"
                     /locus_tag="VE486_01300"
     CDS             complement(2584..3615)
                     /gene="plsX"
                     /locus_tag="VE486_01300"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZA37755.1"
                     /translation="MKIALDAMGGDFGPPNLVAGAVMALREYRLINKLYLVGDSTRIE
                     SELTKHSCNDSRIEIVHATQVVDMSDRAWSAVRRKKDSSVSRAVDLVKHGQADAIVSA
                     GHTGAAVAASTIKLRTLPGIDRPGIAAVLPTETNVFVLIDAGANIDARPEHLLQYAFM
                     GTVYSRHVLHYKNPTVGLVSLGDEDVKGTELTKEVFKMLKPSSLNFVGNIEGRHLFED
                     PVEVVVCDGFVGNVILKTCESIAVAMFQWLKHELMRTTLRKAGAFLARNAIGTIKSKT
                     NYEEYGGSPLLGVNGICIIAHGASTPLAVKNALRVAAESIEQQVNPHIVEEIRRYHET
                     TASLEPALR"
BASE COUNT          764 a         1047 c         1036 g          853 t
ORIGIN      
        1 ggtggagcgc attcggatgc acccgtagct tgcgggcaag ccaatattcc gttcttccgg
       61 cgtgccatga atgtaaatcg tccgcgcata tgcattggca ttttgcgtct cgcggccacg
      121 cagccaaagg attcgtgtca cgattggatc acgacctggt gaatcgattg caacaatctc
      181 accagtccga cgccggtctt tgaagacagc gccgggcgga gcatggtcgc cgactttctt
      241 cgcgacctcc agctcgccca atggagtaaa agaactgcca ggctggtctc cgaggccgaa
      301 cttggatgtt gagaccggat agacggccat caggttaccg cgatcgagaa gagctaactt
      361 ttgctcacgc gcgctgataa cgatctgatg ccgtgtatca ggcgtggcgc acgatgttaa
      421 gcaaaagatt gcaagagtga cgagagcgac taacgaaatc gtgtcgcgcg caatctctcc
      481 ggggaacgca ggctgccagc ctgccgttcg cggccgctgg ccgcgaatca ttgcggtctc
      541 gctggaaaag aacgacgccg gtctaacggg ccgcggcgct gcggatgcgt ccggcaagct
      601 gccggacgcc acaggctggc agcctgtgct ccccggaatc gtcaccctca tcgtccggtg
      661 tttcatcgat tgaactggaa aaacttttct gcggtttcgg aagttgcttc tgcgatttcc
      721 tcaagcgaaa tgccgcgtgc cgccgcgatt gtttcggcga cgatgagtgt gtgagccggt
      781 tcgcagcgtt tccctcgata cgggactggc gcgagatacg ggcaatctgt ttccaccatg
      841 aacttccaga gcggcacctt cgacgccacg tcacgcacgt tcgctccgtt tttgaaagtc
      901 acaacgccag tgaacgaaac cagattatca agatcgagaa cttcgtttgc ctgttcgagc
      961 gtgccgccga agcaatggaa caccccccgc acctttcctg catacggtct catgatttcg
     1021 agcgtgtcat cccacgcatc gcgctggtgg atgaccacat tgaggcccag ctcgaccgcc
     1081 agatcgagtt gttcctcgaa aagatcggcc tgtttcgatt tgtaggcgcc atcttcaatg
     1141 ctggcttcca cttgttcttc ggttccgctt tgcagcgcgc tgaacacctg cacatttttc
     1201 tgtttcgcgg cttcgacgct cggcaaatga tgataatcaa gaccggtctc gccgatcgcc
     1261 accacgcgcg gactcttcgc cagctcccgc aacggtgtaa tgacgtcctc cccggcctgt
     1321 tcggcattgg tgggatgaac gccgattacg gcatagatat tcgggtactt ctcagcgaga
     1381 ccgaccgcgc gccggctgct ctcgattgag gtgccgattg tgatgatacg ggtcacacca
     1441 gcttcggtcg cccgccgcag cacatcctcg aggtcagcag caaactccgg ataatcgaga
     1501 tgcgcgtggg tttcgattag cattggatga attgtagggc gtttctgtga aacgccaact
     1561 tctggttcgg cgtgcgccgc ggcggacgcc ctgcactttt accattcgag cactgtactc
     1621 gcccacgtga gccctccgcc gaagacgacc atcagaacgt aatctcccgg cttaatccgc
     1681 ccactgcgat tggcttcatc cagcgcgatc gccaccgctg cggccgaagt gttgccgtag
     1741 cgatcgagat tcacaaacat cttgtcgcgt gaaatgccga gccgatcggc gatcgcttcg
     1801 atgatacgca cattcgcctg gtgcggaata acacaagcaa tgtcctcgat agagagtccg
     1861 gcctgatcga gggccttctt agcggccttg agcatcgcgg ttactgcctg cttgtaaact
     1921 tccttcccca tcatgtggat cgtttgcaag ttcagatcga tattttcgcg agtgatcggg
     1981 cagcgcgaac cgcccccggg catgaataaa atatccgtat actgaccatc gctgccaatg
     2041 tgcgtgctga ttactccgtg agcgctgccg cgatggcgca ggatggcggc accagccccg
     2101 tcgccgaaaa gcacgcaggt gttgcgatcg gtccaattag taatcgatgt caatttctcg
     2161 gccccgatta caaggacagt atcgtgcgta tgtgaggtga taaattgctg cgcgatctcc
     2221 agcccgaaaa gaaatccggc acaggctgcg gagatatcga gacaagcggc gttggttgct
     2281 ccgatctttt tctggacgaa acaggccgtc gcgggaaaga gcatatcggg ggtggccgtt
     2341 gctacgagaa taagattaat ctctttgggc gaaatctttg cctgttggat tgcctttagc
     2401 gccgctttcg tggccatgtc gctcgtgttt tcctctttcc cggcgatccg gcgttcctta
     2461 atgccggtgc gggtcgtgat ccagtcgtcg ctggtattga ccatgcgcga gaggtccgcg
     2521 ttggtcagaa ttttttcggg cacataacta cccgtgccga tgattgaaac ggtgcgcagc
     2581 ggtttagcgg agcgcgggct cgagtgaggc tgtcgtttca tggtaacggc gaatctcctc
     2641 gacgatatgc gggtttacct gctgttcaat ggactctgcg gccacccgca acgcgttctt
     2701 gaccgcaagc ggcgtgctgg cgccatgcgc aataatgcag atgccgttga cgccgagaag
     2761 gggactgccg ccgtactctt cgtagtttgt tttgctctta atggttccaa tcgcgttcct
     2821 tgcgagaaac gcaccggctt ttcgcagtgt tgtgcgcatc aactcatgct tgagccactg
     2881 aaacattgct accgcgatcg attcgcacgt tttcaagatt acattaccga cgaaaccgtc
     2941 gcatacgaca acttcaaccg gatcttcaaa gagatgacgg ccttcgatgt tgccgacgaa
     3001 gttaagcgaa ctgggtttta gcattttgaa cacttccttg gttaactcgg tccccttcac
     3061 atcctcgtcg ccgagcgaga ccaaaccaac ggtcggattc ttgtaatgga ggacatgacg
     3121 cgagtagacc gtgcccatga acgcgtattg aaggagatgt tcggggcggg catcgatgtt
     3181 tgcgcctgca tcgatcaaaa cgaagacatt cgtttcagtg ggaagcacgg cggcgatacc
     3241 cggtcgatcg attcctggca aggtgcgaag cttgatcgtg ctcgctgcga cagccgcgcc
     3301 ggtgtgaccc gcgctcacga ttgcatcggc ctggccgtgc ttcacgagat cgaccgcccg
     3361 gcttacagac gaatccttct ttcgcctgac cgccgaccat gcgcggtcgc tcatatcaac
     3421 tacttgcgtc gcatgcacga tctcgatccg gctgtcgttg cagctgtgtt tggtgagttc
     3481 gctttcgatc ctggtcgagt caccaacgag ataaagctta ttgattaatc ggtattcgcg
     3541 cagcgccatc acagcgccag ccacgagatt gggcgggcca aagtcaccgc ccatagcatc
     3601 gagggcgatc ttcatcagtg ggaaattttg cgagctaaaa tggctgattg caaagcgaaa
     3661 aatgagagac ggcgtaaatc gtgaatcgga accgagttaa
//
LOCUS       DASQUQ010000048         4560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_278688, whole genome shotgun sequence.
ACCESSION   DASQUQ010000048 DASQUQ010000000
VERSION     DASQUQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278688"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VE486_01305"
     CDS             complement(<1..276)
                     /locus_tag="VE486_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37756.1"
                     /translation="MRTLFFGIAVVLASCAQPEKLVAPSSAAAAGSPFDQVGKVKLYP
                     GQPCTSQIMFLFRADRPVSTISLAAPMPETRILTEAANSRDRVHVRGK"
     gene            complement(450..956)
                     /locus_tag="VE486_01310"
     CDS             complement(450..956)
                     /locus_tag="VE486_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HZA37757.1"
                     /translation="MIPLSFLSPKTEVRESKIHGRGLFATADIAKNEIVAVKGGHIVD
                     RKTLRENITPQLGPVEIQIDDDLFIAPVTGEEQEGSMLYSNHSCDPNIGMRGEITFVA
                     MRDIRGGEELTHDWATTDDDGYSVECKCGSSNCRKILTGKDWQRPNLQKRYAGYFSAY
                     LARKIARR"
     gene            1108..2418
                     /locus_tag="VE486_01315"
     CDS             1108..2418
                     /locus_tag="VE486_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HZA37758.1"
                     /translation="MKSRPAALPFIFVTVMLDMLALGLIAPVLPKLILNFLGGNATSA
                     ATWIGVFGTVFALMQFFFSPVLGVLSDRFGRRPVILLSNLGLGLDYIVMALSPTISWL
                     FVGRVVSGITASSIPTAMAYIADVVPREKRAGAFGMIGVAFGLGFILGPAIGGPLGDI
                     SPRLPFWVAAGFSLANWLYGYIFVPESLRQEHRQEVTLRRANPIGSLVLLRSHPELWK
                     LATLQFLAYIAHEVFVIWALYAIYRFAWNQTTIGISLALVGVFAAAISGGLTGRIVAW
                     LGERRTLYIGQFFGAVGMVIAGLARTGTFYMISIPVISMWNISFPAAQGMMTHRVSER
                     EQGELQGAIQSLRSIAFVIGPFLFSGIFAWFINPKHSVYVPGAPYYLAAALLFTAMLL
                     ATRVEQPQFAEGSPAGPSEAISPDGVASGNPAVAGLIDPSKENT"
     gene            2452..2847
                     /locus_tag="VE486_01320"
     CDS             2452..2847
                     /locus_tag="VE486_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37759.1"
                     /translation="MNTGARCVIGVLAICSWLAISNHCAFAAVATKSEPAESECPFHS
                     KPAKPQNPSPGIQCCKILRAVVPVVTKSWARDDAKFSDFDLHSEECALIPYLHNTPPP
                     LLLDTGPPGKTAFAELVRTSLGRAPPFFV"
     gene            3005..>4560
                     /locus_tag="VE486_01325"
     CDS             3005..>4560
                     /locus_tag="VE486_01325"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HZA37760.1"
                     /translation="MRKQISILIAATTAAATTAFSQAGTAHQKDREHNYEHPPSVAAA
                     TSGAAAEQEKKQKYTCPMHPEVVMDHPGNCPKCGMKLEPMKSKTHETHGTHAAHETHE
                     THTSHELHRQMSMQSSVNIADPMSRESSGTSWVPDSTPMYGKMFMFGEDMLMLHGAIF
                     PRYTNVSTRRGDDRIDAPNWIMGMYSHPLGQNAQFGARLMMSLDPLTEGGRGYPLLFQ
                     TGESWQGRALHDRQHPHDLFDELSISYSQKFNSDLSAYLYFGYPGEPALGPPAFMHRL
                     SAMDDPDAPISHHWQDSTHITFGVATAGLAWSRFGGMKVEGSIFTGREPDENRYDFDR
                     PRFDSYSGRISWNPTKNLALQVSHGYLKSPEALEPDLKLHRTTASLIYNVPLGNDSNW
                     SNSFVWGQNRATGEGKTQSFLIESNYQRGRDTVYARWERVEKSGHELVLDHADESRIF
                     PVSGYTLGYVHDLTHGSGLDIGLGTQFTVNDRPDSLDRYYGDDLGYAFQFFLRIRPSL
                     HAHGSLEHSE"
BASE COUNT          954 a         1295 c         1254 g         1057 t
ORIGIN      
        1 ttttccgcgg acatggacgc ggtctcgact gtttgctgcc tcagtaagaa ttctcgtttc
       61 tggcatcggc gcagccagcg agattgttga gaccggtcgg tccgcacgaa aaaggaacat
      121 gatttgtgac gtgcacggtt gtccgggata caatttgact ttccccactt gatcgaaggg
      181 agaaccggcg gcggcagcgc tggacggcgc caccagcttc tccggttgag cgcagcttgc
      241 cagcacaacc gcaattccga agaaaagcgt acgcaagggc ccactcatat tcggcgattt
      301 ttcttggaca aactattcac cgttccaggt gaacttaccc acgatttttc cacttttttg
      361 tttgcggaca agcaagatta accatctcat cctggcgctg gaaccgacgg cgaacaatca
      421 gatcttgaac accctgtaaa atcgatcagc tagcgccgcg cgatctttct ggccaaatac
      481 gcggagaaat aaccggcgta acgcttttgc aaattgggtc gttgccaatc tttgccggtc
      541 agaatcttgc ggcaatttga agagccgcat ttgcactcga ccgaatagcc atcgtcatcg
      601 gtggttgccc aatcgtgagt tagttcttca ccaccgcgga tgtcgcgcat ggctacgaaa
      661 gtaatctcgc cgcgcatgcc gatgttcggg tcgcacgaat ggttgctgta aagcattgag
      721 ccttcctgct cttccccggt gaccggcgcg ataaagagat catcgtcgat ttgaatttcc
      781 accgggccaa gctggggagt gatgttctcg cgcaaagttt ttcgatcgac aatgtgcccg
      841 cctttcaccg ccacgatttc gtttttggca atgtcggcgg tcgcgaacaa accgcgcccg
      901 tggattttac tctcgcgcac ttccgttttc ggcgagagaa acgaaagcgg tatcatcgga
      961 atcagctaca gattagcacg gtttggcagg gtcagcgcag cgcgctgacc cccgaaagcc
     1021 ttcggtgtag cgcggcgtcc ctaccattga ggactgaaat ccgcacttga atgccatgcg
     1081 atccgcgact tcgatgtcga tctcgcaatg aaaagccgcc ccgccgccct cccatttatc
     1141 ttcgttacgg tcatgctcga catgctggcg ctggggctga tcgcgccggt tttgccaaag
     1201 ctcattctga attttcttgg cggtaacgcg accagcgcag caacgtggat tggcgtgttc
     1261 ggaacagttt tcgcgctgat gcagtttttc ttctctccgg tattgggcgt gttatcggac
     1321 cggttcggac gacgaccggt aatcctttta tcgaatctcg gtctcggctt ggattacatt
     1381 gtgatggcgc tctcgccgac cattagctgg cttttcgttg gccgggtcgt ctccggaatc
     1441 accgcctcca gcatcccgac ggccatggcc tatatcgccg acgtcgtccc gagggagaaa
     1501 cgcgccggcg cttttgggat gatcggcgtc gctttcggtc tcggctttat tctggggccg
     1561 gcaatcggcg ggccccttgg ggacatcagt ccgcgtttgc cattttgggt tgcagccgga
     1621 tttagcctgg ccaactggct ctatggctac attttcgttc ccgaatcgtt gcggcaggag
     1681 catcgacaag aggtcacgct ccgtcgcgcc aatccaattg gatcgctggt gttgcttcga
     1741 tcacatcccg aactgtggaa actcgccacc ctccagtttc ttgcctacat cgcgcatgaa
     1801 gttttcgtca tttgggcgct ctacgcgatt tatcgcttcg cctggaacca aactacaatc
     1861 ggcatttccc ttgcgctggt tggcgtgttc gcggccgcga tctccggcgg gctgaccgga
     1921 cgaatcgtcg cgtggctggg tgaacggcgc acactttaca ttggccaatt tttcggcgca
     1981 gtgggaatgg tcattgctgg tctcgccagg accggcacgt tctacatgat ttcgatcccg
     2041 gtgatttcga tgtggaatat ttccttcccg gcagcgcagg gcatgatgac tcaccgggtg
     2101 agcgagcggg aacagggcga attgcaaggc gcgatccaga gcctgcgttc catcgcgttc
     2161 gtcattgggc catttctgtt ttccggaatc tttgcctggt tcatcaaccc gaaacattcg
     2221 gtttatgtgc ccggggcgcc gtattacctt gcagccgcgt tgctcttcac ggcaatgttg
     2281 ttggcgacgc gagttgagca accgcagttt gccgaaggca gcccggccgg accgagtgag
     2341 gccatttctc cggacggggt cgcttcggga aaccccgccg tcgcggggct gatcgatccg
     2401 tcgaaagaaa atacttgagt catcgcggtg gttcatcgaa ttgaaggagc gatgaataca
     2461 ggcgcgcgct gcgtaattgg ggtgcttgcg atttgctcct ggctcgcaat ctcgaatcat
     2521 tgcgcgtttg ccgccgtcgc aaccaaaagc gagcccgcag aaagtgaatg cccatttcat
     2581 tccaaacctg cgaaaccgca aaatccttca ccgggaattc aatgctgcaa gattttgcgc
     2641 gcggtcgttc cggttgtgac gaagagttgg gcgcgcgacg atgcgaaatt ttccgatttc
     2701 gatcttcatt ccgaagaatg cgcgctcatc ccgtacctgc ataacacgcc gccgccgttg
     2761 ttgctcgaca ccggacctcc tggaaaaacg gcattcgctg aattggttcg gacctcgctg
     2821 gggcgagctc ctccattttt cgtctagggt agacgcggct ggatccgccg taattttcca
     2881 tcgggggcga cgcgcccgcc gctacaccaa gcaattgtag ggcaaccgct ccggttgccc
     2941 tgtttcccgg caggcgaacg cgcctcccta caaccgcatt tgctaagacc gacatgagaa
     3001 aaacatgaga aaacagattt caattttgat cgccgcgaca acggctgcgg ccacaacagc
     3061 tttctcgcag gcagggacgg ctcaccaaaa agatcgagag cacaattacg agcacccgcc
     3121 ttcggtcgcc gcggcgacct ccggcgcggc agcggagcaa gagaagaagc agaaatacac
     3181 ctgcccgatg catccggaag tggtgatgga tcatccgggc aactgtccga agtgcggcat
     3241 gaaattggag ccgatgaaat caaaaactca tgagacgcac gggacccatg cggcacatga
     3301 gacccatgag acccatacgt ctcatgagct ccatcggcag atgtcgatgc aatcctcagt
     3361 aaacatcgcc gatccgatga gtcgggaaag ttcaggcacg tcatgggtgc cagattccac
     3421 gccgatgtat ggcaaaatgt tcatgtttgg cgaagacatg ctgatgttgc acggcgcaat
     3481 ttttccgcgt tacaccaatg tgagcacgcg gcgcggcgat gaccggatcg atgctcccaa
     3541 ctggatcatg ggcatgtatt cccatccgct tggtcagaat gcgcagttcg gtgcgcgact
     3601 aatgatgagt ctcgacccgc tgactgaagg cgggcgcggt tatccgcttc ttttccaaac
     3661 cggcgaatcg tggcagggtc gagcgctgca cgatcggcaa catccgcacg atctgtttga
     3721 tgaattgtcg atcagttact cacagaagtt caattccgat ctgagcgcgt acttgtattt
     3781 tggttatccg ggcgaaccag cgctcgggcc acccgcattc atgcaccggc tttcggcaat
     3841 ggatgatccg gacgcgccga tcagtcatca ctggcaggat tcaacgcata tcacgtttgg
     3901 cgtcgccacg gccggtctgg cttggtcccg cttcggcggg atgaaagtcg agggcagcat
     3961 ttttactggc cgggaaccgg atgaaaatcg ttacgatttc gatcggccac gattcgattc
     4021 atacagcggc cgcatttcat ggaatccaac caaaaatctg gccctgcaag tttcgcacgg
     4081 ttatctcaag agtccggaag cgctagagcc cgatctcaaa ctgcatcgca caaccgcgtc
     4141 gcttatttat aacgtgcccc ttggaaacga tagcaactgg tcgaacagct ttgtctgggg
     4201 ccaaaaccgg gccaccggtg aggggaaaac gcaatcattc ctcatcgagt cgaactatca
     4261 gcgcggacgc gacaccgttt acgctcggtg ggaacgcgta gaaaaatcgg gccatgaact
     4321 cgttctcgat cacgccgatg aatcgcgaat ctttccagtg agcggttaca ccctcggtta
     4381 cgtgcacgat ttaacacatg gcagcggtct ggatatcgga ctcggcaccc aattcacggt
     4441 aaatgatcgc ccggacagtc tcgaccgcta ttacggagac gatctcggtt acgcgtttca
     4501 gtttttcctg cgcattcgcc catcgttgca tgctcacggc tcgctcgaac attccgagca
//
LOCUS       DASQUQ010000049         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_188018, whole genome shotgun sequence.
ACCESSION   DASQUQ010000049 DASQUQ010000000
VERSION     DASQUQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188018"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VE486_01330"
     CDS             <1..538
                     /locus_tag="VE486_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37761.1"
                     /translation="WLVCALAERDAAAATDALSKLDDNEIFLGVQVQLNRPFVEGLIA
                     RMTNDEHKAQLAFTAARAEQEKIVQAQPDFGPAWCVLGLIDAGLGRKEEALREGRRAI
                     ELLPMEKDARVGNYLVGYFAVIAAWVGEKDLACEQLAIVTRPPGAVSYGGLKLLPFWD
                     PVRGERCFEEIVASLAPK"
     gene            857..1252
                     /locus_tag="VE486_01335"
     CDS             857..1252
                     /locus_tag="VE486_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37762.1"
                     /translation="MIAISGTARADGDWRRGCGLQTLRGSYVFNPHGFNIVNGVAQPV
                     AVFEGIDFNGDGTLTVPFATVSVNGLILHFPPGGTGTYTLNQNCQGTLTFAAGPISFD
                     IFVRAHGKEIEMIRTDANTVLNGTAKRVR"
     gene            1290..1478
                     /locus_tag="VE486_01340"
     CDS             1290..1478
                     /locus_tag="VE486_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37763.1"
                     /translation="MKTISSLSAIFVIALFASPAPAGQQVPLNGSFTSNETYDALMLT
                     NLLEREGAQSWRKSYAFT"
     gene            complement(1936..>2916)
                     /locus_tag="VE486_01345"
     CDS             complement(1936..>2916)
                     /locus_tag="VE486_01345"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZA37764.1"
                     /translation="LHEVLDTIRQVAPSSANVLIEGESGTGKELAAHAIHNLSRRNKA
                     KFVAVHCAALSPQLLESELFGHEKGAFTGAHERRIGRFEQANGGTIFLDEIAEIDSST
                     QVKLLRLLSEKRAFERVGGNQTLRADVRLIAATNKNLEQLVREGKFRDDLFFRLNVVR
                     ITMPPLRERKEDIPILVRGFLRHFCKANDKQLLELAPDAMDALLTYNWPGNVRELRTA
                     IEHGVVMATGPKIILRDLPMAVRQAARATSPRGVSATEAFGEKSSPLDLHETERKLIM
                     QALATTNGNVTAAAKKLGISRRTLHRRINEMNVARTKAEASDASEDQQSA"
BASE COUNT          658 a          824 c          806 g          628 t
ORIGIN      
        1 ttggcttgtt tgtgcactgg ccgaacgcga cgccgctgcc gcaacagatg ccttgagcaa
       61 gctggacgat aacgaaatct tcttaggcgt tcaggttcaa ttgaatcgcc catttgtaga
      121 aggtctcatc gcgcgcatga caaacgatga gcacaaagcg cagttggctt ttaccgctgc
      181 gcgcgccgag caggagaaaa tcgtccaagc tcaacccgat ttcggcccgg cgtggtgtgt
      241 gctgggtttg atcgacgctg gactcggccg gaaagaggaa gcgctgcgtg aaggccggcg
      301 ggcaattgag cttcttccca tggaaaaaga tgcccgcgtt ggcaactatc tggtcggcta
      361 ttttgcagta attgcagcgt gggttggaga aaaggatctc gcttgcgaac agctggcgat
      421 cgtcacccgt ccacctggcg ccgtcagcta tggcggattg aaactgctgc cgttctggga
      481 tccggtgcgc ggcgagcggt gcttcgagga gatcgtcgcc tcgctcgcgc cgaaataaac
      541 ttcaccacgg attacaccga taatacggat caagagatgg tagggcgcga ccgccgggcg
      601 cgccgaatcg ctaaatccaa tccgtgctat ccgcgtcatc cgtggttctc aagtttccaa
      661 actttccgcg taaccttccg ccggatttta cgcattacag cgtgaacggc cagacctgac
      721 ttctcaataa cgaggagtca aagaaggccg aaaccaaaca aaaccaaagg aaaaccaaaa
      781 tatgaaaaga ctaatgaaga gaataatcgg caccctaacc tccgcgagtt cgctggcgag
      841 cctcctcggc ttggcgatga tcgcaatctc cggcaccgcg cgggccgacg gcgattggcg
      901 acgtggttgc ggccttcaga cgctacgcgg ctcgtatgta ttcaacccgc acgggttcaa
      961 catcgtcaat ggcgtcgccc aaccggtcgc cgtctttgaa ggcatagact tcaatggaga
     1021 cgggacgctg accgttccct tcgccaccgt gagcgtaaat ggcctcatac tccactttcc
     1081 tcctggcgga acgggcacct acacgcttaa tcaaaactgt caaggcaccc tcacatttgc
     1141 agcagggccg ataagcttcg acattttcgt ccgagcccac gggaaggaga ttgaaatgat
     1201 taggaccgat gccaacaccg tcttgaacgg cacggcaaaa agggtgcgct aaagagacca
     1261 aaacaactgg agaaaatcaa aatcgaacta tgaaaacaat cagctcacta tcagcaatat
     1321 tcgtgatagc gctcttcgcc agcccggcgc cggcaggcca gcaggtaccc ttgaacgggt
     1381 cgttcacatc aaacgaaacc tacgacgcgc tcatgctcac gaatctcctc gaacgggagg
     1441 gcgcacaaag ctggaggaag agctacgcgt tcacctgata atatgggccc tactttctga
     1501 tctcgggctg atcgatgcag ccttgggaag aaaaggaggc gcggagcgag ggccggcaac
     1561 ctcctcacaa cggctgcgcc accgaaatag gcacgcgcta ttctgatcgc tccaatgccg
     1621 ttcagtgtcg caacgaactt tgcggcgccc aagtcgcaat tgaatgccct aggactgttg
     1681 gcacgagatt cgacttctga tcctgatcac ggggtttggc gcgctccatc cgcgccaaac
     1741 ccctagccac gaacaaacgc ggagatgtgt tgaggactcc gagggctcgc gagcagccgc
     1801 gttaaggaaa caatccgcca aacttgccaa aacctgtggt gagcggagcc gaatccatga
     1861 cgccaaaata aaggctgtaa ttctgacttc agatctttaa attttgaact gttacatccg
     1921 taacattgaa acgagtcacg cagattgttg gtcctcactt gcatctgacg cttcggcctt
     1981 cgttctggct acattcattt cgttgattct tcgatgcagt gttcgccggc tgatgccgag
     2041 ctttttggca gcggcggtca cgttgccatt ggtcgtggcg agcgcttgca tgattaattt
     2101 tctctccgtc tcgtgcagat cgagcggact cgatttttcg ccaaaagctt cggtggcgga
     2161 aacaccccgc ggcgatgtcg ctctggctgc ctgtcgcacc gccattggga gatcgcgcag
     2221 aatgattttt ggaccggtcg ccatcacaac tccgtgttcg atcgcggtgc gcagctcgcg
     2281 aacgttgccg ggccagttgt aggtgaggag cgcatccatc gcatccggcg ccagctcgag
     2341 aagctgtttg tcgtttgcct tgcagaagtg tcgcaaaaat ccgcggacaa ggatcggaat
     2401 gtcttccttt cgttcgcgca acggcggcat ggtaatgcgc acgacgttga gacgaaagaa
     2461 cagatcgtcc cgaaactttc cctcccgcac gagttgttcg aggttcttgt ttgtggccgc
     2521 aatcaggcga acgtccgcgc gcagcgtttg attgccgcct actcgctcga aggcgcgctt
     2581 ttcgcttaat aagcgcagta atttgacctg cgtgctcgaa tcgatctcag cgatttcgtc
     2641 gaggaaaatg gtgccgccgt ttgcctgctc gaatcggccg atgcggcgtt catgcgctcc
     2701 ggtgaacgca cctttctcgt gcccgaaaag ttcgctctcg agcaattgcg gcgagagcgc
     2761 cgcgcaatgc acggccacaa atttcgcttt gttgcgccgg ctcaaattat gaatggcatg
     2821 ggcggccaat tcttttccgg taccgctctc gccttcaatg agcacattgg ccgaggaggg
     2881 cgcgacctgc cggatcgtgt cgagcacttc gtgcaa
//
LOCUS       DASQUQ010000050         5502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_551089, whole genome shotgun sequence.
ACCESSION   DASQUQ010000050 DASQUQ010000000
VERSION     DASQUQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551089"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1779)
                     /gene="proS"
                     /locus_tag="VE486_01350"
     CDS             complement(250..1779)
                     /gene="proS"
                     /locus_tag="VE486_01350"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZA37765.1"
                     /translation="MNTTSTTKTAITPRRNEDFPEWYQQVIRGAELAEPSDVRGCMVI
                     RPWGYGIWENMQRQLDAMFRATGHRNAYFPLFIPLSFFAKEAQHVEGFAKECAVVTHT
                     RLETDAEGKLRPSSALTEPLVVRPTSETIIGASYAKWVQSYRDLPILINQWANVVRWE
                     MRPRLFLRTTEFLWQEGHTVHESEAEARAETKLILDLYERFVRDHLAIPVFTGEKSES
                     ERFPGAVQTLSIEALVQDRKAIQAGTSHFLGQNFARASGIQFQNREGKQEFGWTTSWG
                     VSARLVGTVVMEHSDDDGLVLPPRIAPTQIVILPVTPKEETRTAVIEACDKLAKQLRE
                     SRYVDLPIEVEVDRRNLGGGVKNWEWIKKGVPLRLEIGPRDLASGNVMVSRRDQPVKA
                     KESMPMEELVSRAPEILMSIQNTLYERAKKFRDANTRTIDSKKEFYDFFTPKNSAKPE
                     IHGGFALAHWNGSRAVEEQVKNDLKVTVRVIPFDESAEAGRCIFTGEPSARPVVWAKS
                     Y"
     gene            2152..2583
                     /gene="rplM"
                     /locus_tag="VE486_01355"
     CDS             2152..2583
                     /gene="rplM"
                     /locus_tag="VE486_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZA37766.1"
                     /translation="MKTFSAKASEVPRKWWVIDARDQVLGRVAVKAANLLRGKEKTIL
                     TPHVDTGDFVVVINAEKVRLTGRKENQKSYMSFSGYVGGHKSENVQQRRVRHPELLIE
                     RAVRGMIPHNRLGRAIYRKLKVYRGDSHPHAAQQPHAVKLA"
     gene            2594..2989
                     /gene="rpsI"
                     /locus_tag="VE486_01360"
     CDS             2594..2989
                     /gene="rpsI"
                     /locus_tag="VE486_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZA37767.1"
                     /translation="MPEAEEFTATGRRKTSVARVRMAPGSGRIDVNGRSFEDYFPTVP
                     LQNVVLQPLQTAKAVNAYDLSINACGGGATGQAGAVQLAIARALLQVDGNLRGALKTE
                     GLLRRDPRMKERKKSGQPGARKRFQFSKR"
     gene            complement(3139..3882)
                     /locus_tag="VE486_01365"
     CDS             complement(3139..3882)
                     /locus_tag="VE486_01365"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA37768.1"
                     /translation="MPTVPPPSRDAALETRVFWERFKREIIAALIILLIALFGFTGYR
                     FYSDRRAATASALLASAKGAQEYEQVIARYPNTPAAADAYLLLAQAQRSERKFAEANA
                     TLQAFITKNPNHEFVSTARMAMAANLESMGKRDDALAMYQQIASSYPNSFNAPLALLS
                     QVYLLKAKNRDDEARRISETILTQYRTSFWAGEAFQQLRLLKPSVPSEPAAAAAPTIP
                     PFLTAPAPMPEAEDVPKTAPTTPVASATP"
     gene            4219..4725
                     /locus_tag="VE486_01370"
     CDS             4219..4725
                     /locus_tag="VE486_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HZA37769.1"
                     /translation="MNAFWNSFETLLGLGVHPEDLTFVQISLRGILVFLATLMMVRFG
                     HKRSLARKTPFDAILLVILAAVLSRAINGSAPFLATIGGGVVLVLLHRLFAHLAYRSH
                     RFGILVKGSPDTIVRNGECDLRMMRRNHVSMHDLEEDMRLHAHIDDLSKIRLARVERS
                     GDISFIKS"
     gene            complement(4762..>5502)
                     /locus_tag="VE486_01375"
     CDS             complement(4762..>5502)
                     /locus_tag="VE486_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZA37770.1"
                     /translation="DLNPIEEAQGYARLASEFRMRQEDIALKVGRSRAAVANAMRLLD
                     LHPQVPVIIRSASDLEVLELSLIENLQRADLNPIEEAQGYARLASEFRMRQEDIALKV
                     GRSRAAVANAMRLLDLHPQVQIWLAQDLLSVGHAKVLLALKAPEEQLLAAETVLRRNA
                     TVRSTERLVARQLGIGRFRRRSRRVGPEQAVSSAAIEDLQNRLQERLATHVTIHHGEK
                     RGRIEIEYYGNDDLQRIATALGLTPSES"
BASE COUNT         1143 a         1570 c         1540 g         1249 t
ORIGIN      
        1 cttcgcgtca atgcaccacg agcaccattc gcgactgtaa agcttcaatt ccacggcgac
       61 cgcgggaata aacagaagcg gagtgcgccg tcaataccta tggcgtaggg atcccggcta
      121 aagtttggag tcccgcgttt acgcggtcgg gtcgcaagtc atggcagacc gcgtaaaggt
      181 tcggaactcc gaacctcctg ccagcttcac gggaagagtt ttacgcctcg ccactttatc
      241 gacaatgcct cagtaggatt ttgcccagac gaccggacgc gcgcttggtt ctccagtaaa
      301 aatgcagcgg cccgcctcgg ctgattcatc gaatggaatg acgcggacgg tcactttgag
      361 atcgtttttg acttgttcct cgactgcgcg agaaccgttc cagtgcgcga gggcgaagcc
      421 accgtggatt tccggtttgg cggaattttt cggagtaaag aaatcgtaga actctttttt
      481 cgaatcgatc gtgcgtgtgt tcgcatcacg gaattttttc gcccgctcat acagagtgtt
      541 ctgaatcgac atgagaattt ccggcgcgcg cgataccaac tcttccattg gcatcgattc
      601 cttcgccttc accggttgat cgcggcggct caccatcacg ttgcctgatg cgagatcgcg
      661 tggcccgatt tcgaggcgca gtggcacacc cttcttgatc cactcccaat tcttgacacc
      721 gccaccgaga ttacgccgat cgacttccac ttcgatcggg agatcgacgt atcgactctc
      781 gcgcaattgt ttcgcgagtt tgtcgcaagc ttcgatcacc gcggtccgcg tttcttcttt
      841 cggcgtgact ggcagaatca cgatctgcgt tggcgcgatc cgcggcggca acaccaggcc
      901 gtcgtcgtcg ctgtgttcca tcacgaccgt gccgacgagg cgggcgctca cgccccagct
      961 tgtggtccag ccaaattcct gtttgccttc gcggttctga aattggatac cgcttgctcg
     1021 cgcaaaattc tggccgagaa aatgcgaagt gccagcctgg atcgccttcc ggtcctgcac
     1081 cagcgcttcg atcgataatg tctgcaccgc gccggggaaa cgttcgctct ccgatttttc
     1141 gccggtgaaa accggaatcg cgagatggtc gcgcacgaac cgctcgtaaa ggtcgagaat
     1201 taacttcgtt tcggcgcgcg cctccgcctc actctcatgg acggtgtgtc cctcctgcca
     1261 gaggaattcg gttgtgcgca aaaacaaacg cggccgcatt tcccagcgga caacgttcgc
     1321 ccattgatta atgaggattg gcaagtcgcg gtaggattgc acccatttgg cgtagcttgc
     1381 gccgatgatc gtttccgagg taggccggac cacgagtggc tccgtcaacg cgctcgaggg
     1441 ccgcaatttt ccctccgcat ccgtttccag ccgggtatgg gtgaccacgg cgcattcttt
     1501 ggcgaaacct tcgacgtgtt gcgcttcctt ggcgaagaag ctaagcggaa taaaaagtgg
     1561 gaaatatgcg ttgcgatgtc cggtcgctcg aaacatcgca tcaagctgcc gctgcatatt
     1621 ctcccaaatg ccgtagcccc agggacggat caccatgcaa ccgcgcacat ccgagggctc
     1681 ggccagctcc gcaccgcgga tgacttgctg ataccattcg ggaaagtcct cattgcgccg
     1741 cggcgtgatc gcggtttttg ttgtggaagt cgtattcatc gtgagaggca attgtagggc
     1801 ggctgtgtca gccgccaact gggattttat tatgcccgtt gccgccgcaa gatggccagt
     1861 cgccatttgc caggtccttc tgaaaataat gagagattcc tccagccttc gcatagagct
     1921 acagtttggc aggcgactcc gcttggaatg aaaaagccgc cgaggtatga tcgcggtttt
     1981 tgtgagcgtt gtcgtcggca tcgcaagaga attaagcgcg caaggtcaca ataaccggcg
     2041 agcatttcgg cgatgagacc cgatgcgtcc catatgactc atgggtcgga tggctgtcgc
     2101 tgggttgaca ggcgcctgcg ccgcccgtag ctttcgtgcc ttttccgcag tatgaaaacg
     2161 ttttctgcca aagccagcga agtcccgcgt aaatggtggg taatcgacgc cagggaccag
     2221 gtgttgggcc gggtcgcggt aaaggcagcc aacttactgc gcggcaagga gaagacgatc
     2281 ctcacgccgc acgtcgatac gggggatttt gtggtcgtaa tcaatgcgga gaaagtgcgg
     2341 ttgaccggaa gaaaagaaaa ccagaaaagc tacatgagtt tttctggata cgtcggtggt
     2401 cacaaatccg aaaatgtcca gcagcgccgc gttcggcatc ctgaattatt gatcgagcgc
     2461 gccgtgcgcg gaatgattcc gcacaaccgg cttggtcgcg caatttatcg aaaacttaaa
     2521 gtttatcgcg gtgattccca tccccatgcc gcgcagcagc cacacgcggt caagttagcc
     2581 taaaaggagt cttatgccgg aagccgagga attcactgcc acaggccgcc gcaagacgtc
     2641 ggtcgccagg gtccggatgg cgccgggcag tggtaggatc gacgtcaacg gccggagctt
     2701 tgaagattat tttccgaccg tgccgttgca gaacgtggtg ttgcaaccgc ttcaaactgc
     2761 aaaggcggtc aatgcttacg acctttcgat caatgcctgc ggcggcggcg caaccggcca
     2821 ggccggcgcg gtgcaactcg ccattgcgcg cgctcttctt caagtagatg gcaatttgcg
     2881 cggcgcgctg aagaccgaag gtcttctccg ccgggatccg cgtatgaaag agcgaaaaaa
     2941 atccgggcag cccggcgccc gcaaacgttt ccagttctcg aaacgataat tgtcggaaca
     3001 taggcttcca gcctgtgggg tgagcgggcg tcctcgcctg cgagtggaag cgtgacatcg
     3061 ttggcaatcc ggcagacaag atgtctgcgc tccaagatcc ccgcggcagt tttaccttcg
     3121 cggcggccgt gtcagctttt acggtgtcgc acttgctact ggtgtcgtgg gtgcagtttt
     3181 cggaacatcc tctgcctctg gcattggagc tggcgctgtc aaaaacggcg ggatcgttgg
     3241 tgcggccgcc gctgccggct ccgaagggac gcttggtttc aataagcgca attgctggaa
     3301 cgcctcgccg gcccaaaagc tggtgcggta ttgtgtgaga atggtctcgg agatgcggcg
     3361 cgcttcgtca tcccggtttt tggctttcaa gaggtacact tgcgaaagca gcgccagcgg
     3421 ggcattgaag ctgtttggat agcttgatgc gatctgttgg tacattgcca gcgcatcatc
     3481 gcgttttccc atcgattcca gattcgctgc catcgccatt cgcgccgtgc tcacaaattc
     3541 gtgatttggg tttttggtga taaaagcctg aagggtcgca ttcgcctctg cgaacttcct
     3601 ttcgctccgt tgagcctggg cgagcagaag ataagcatcg gcagcggcgg gagtattcgg
     3661 gtaacgcgca atcacctgtt cgtattcttg cgcgcccttt gcgctggcta acaacgccga
     3721 tgccgtggca gcccggcgat cggaataaaa gcgataacct gtaaacccga aaagggcgat
     3781 cagaagaata attagggcag cgatgatctc gcgtttaaat cgctcccaaa acacacgtgt
     3841 ttcaagcgcc gcatcgcggg aaggaggtgg gactgtcggc ataatttcga tgtgctccag
     3901 gcggtttaaa acctgtcgag cgtgctctta tgccatgcga aagttcctcg cgcaagagcg
     3961 gtaaacggac cgcaattcat tcaacgcctg tcaggcatga ggaaacatgg aaggatgcaa
     4021 tgttcggagc aaatgacgaa gattgaatgg gatcactgtt tctcttttaa caataagaga
     4081 ttcctccaat ccggtcggaa tgacaaaaca agctgaaaaa gctggctttg ggcttcctca
     4141 ttcggcattc ggcattctgg ttgccgcaac ctcggcatgc gcgcgacaaa aatttttcca
     4201 gcgatacact ctcactctat gaatgcattc tggaactctt tcgaaacatt actcggtttg
     4261 ggcgtgcacc cagaggattt gacgttcgtc caaatttcgc tgcgcgggat cctcgttttt
     4321 cttgcgacat tgatgatggt ccgattcggc cataaacggt cgctcgcgcg gaaaaccccg
     4381 tttgatgcca ttctgctcgt cattctcgct gcggtgcttt cacgcgccat caacggctcg
     4441 gcaccttttc ttgccacaat cggcggaggc gttgttctgg tattgctcca tcggctcttc
     4501 gcccacctgg cttaccgctc acatcgtttt ggaatcctgg tgaaaggctc gcccgacacc
     4561 atcgtccgca atggcgagtg cgatcttcgc atgatgcgac gcaaccatgt ctcaatgcat
     4621 gatctggagg aggatatgcg cctccacgcc cacatcgatg acctttccaa aattcgactt
     4681 gcccgtgtgg aacgcagcgg ggacatcagc tttatcaaga gttagccgca cgtcgagacg
     4741 ccggtgcggt gcgtgggaaa gtcagctctc gctcggcgtc agcccgagag cagtagcaat
     4801 ccgctgaaga tcgtcgtttc cgtaatattc gatctcgatc cgcccgcgct tctctccgtg
     4861 atgaatcgtc acgtgcgtcg cgagacgttc ttgcaaccga ttctgtaaat cctcgattgc
     4921 cgcagagcta actgcttgtt caggcccgac gcgtcgggat cttcgccgga aacggccgat
     4981 cccgagctgg cgcgccacca gccgttccgt ggaacgaacc gtggcattgc ggcgcaacac
     5041 tgtttccgcg gccaggagtt gttcctctgg cgccttcaat gcgagcaaga cttttgcgtg
     5101 accaaccgaa agcaaatcct gcgcaagcca gatctgcacc tgcggatgca aatccagcaa
     5161 acgcatcgca ttagccactg ccgcccggct gcgcccgact ttgagcgcaa tgtcttcctg
     5221 gcgcatccta aactcactgg ctaacctagc atagccctgc gcctcctcga tcgggttaag
     5281 gtcggcccgc tgcaagtttt caatcaggga gagttcgaga acctccaggt cgctcgcgct
     5341 ccgaatgatc accggcacct gcggatgcaa atccagcaag cgcattgcat tagccactgc
     5401 cgcccggctg cgcccgacct taagggcaat gtcttcctgt cgcatccgaa actcactggc
     5461 taacctggca tagccctgcg cctcctcgat cgggttaagg tc
//
LOCUS       DASQUQ010000051         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_823414, whole genome shotgun sequence.
ACCESSION   DASQUQ010000051 DASQUQ010000000
VERSION     DASQUQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823414"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..699)
                     /locus_tag="VE486_01380"
     CDS             complement(196..699)
                     /locus_tag="VE486_01380"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZA37771.1"
                     /translation="MLTTLVFVPAAETQKQPAAGTPAPDFSLTTSDGSQINLKDYRDK
                     WVVLYFYPKDFTSGCTMEARNFQRDLAKYEEAGAVVLGVSVDTAQSHKDFCAKEGLSF
                     KLLADPDARISTDYGSVMDYKGEKLAARNTFIINPQGEIAKVYTGVKPAEHSEQVLKD
                     LAELKNG"
     gene            790..1329
                     /locus_tag="VE486_01385"
     CDS             790..1329
                     /locus_tag="VE486_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZA37772.1"
                     /translation="MISRIWTIGHSTRTIDVFISLLEENGIKLVADVRSLPGSRRYPQ
                     FNKETLAESLSEHRIRYEHFPELGGRRRAKKDSHNTAWRNASFRGYADYMETEEFRKG
                     TERLLDLASKLGPTAIMCAEAVWWRCHRALISDYLKAQGAEIIHILDANKRELHPYTS
                     AARIIDGGLSYASEADGLI"
     gene            complement(1326..1880)
                     /locus_tag="VE486_01390"
     CDS             complement(1326..1880)
                     /locus_tag="VE486_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37773.1"
                     /translation="MDISTLANLINAFAVTAGVIFAAAQIRYYRQRRKRDAMLELVRS
                     FQSPAFTSALRRVLLLPDGADAQKIREVLGPDGEDAVYLVSLTWESLGVLVFRRELAL
                     DLVDDFFSGPILLSWRKLHVWAEQWRREQNRETGSEWFHWLAERMMEREKLAPPVPAY
                     IAHRTWREPRRRLASDGSTASDSR"
     gene            complement(1882..3150)
                     /locus_tag="VE486_01395"
     CDS             complement(1882..3150)
                     /locus_tag="VE486_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HZA37774.1"
                     /translation="MKRFLILQVFSALMGAMAHAATSFDLAAKATNQLAVDLHRQLAT
                     DNENLCVSPYSIESALAMTFAGADGETRSEMARVLHFPNDGDAVPASFASLQHSLEEM
                     SAKTAELVKQAKRFGGPSEPITLSIANRLFAQKGYDLRESFLSLVKQNYGAAFEPIDF
                     IADASGATQHINKWVADQTRNRIRDLIPASALNETTRLVLANALYLKASWADPFSEKT
                     TKPEPFHVRGETPVDVPMMRKLDRHFGYAKRDGFAAVSLPYTGNELQFLVLLPDDANG
                     LKALESKLTAEMLTQCAKLEMREIDLHLPKFKIEPPTIALAKQFEALGMKTAFDQPRG
                     SANFDRMAPRTPRDYLYISQIFHKTFIAVDEKGTEAAAATAVAMMAGTALRSPPPPPI
                     EVKVDRPFVYAIQHVPSGVCLFLGCVTDPR"
BASE COUNT          719 a          894 c          890 g          758 t
ORIGIN      
        1 ttctaacagt tcctgtgcgc tgaaaccgac acccgactca aacgccaccg gctcaagtca
       61 aaatttcgct ttcgctcgat cccgctggac atgcacgtgt ggccgctcgt ctcgatcgga
      121 ggaataaaag aaaaggcggt agggaccaga gcgagggacg gtcggcacgg tcgcgaaatt
      181 cggaagatct ctgttctacc cgtttttcag ctccgccaaa tccttcagca cttgctcact
      241 gtgctcagcc ggtttcactc cggtgtaaac cttggcgatc tcgccttgcg gattaatgat
      301 gaaggtgttc cgggcggcca gtttttctcc cttgtaatcc atcaccgatc cgtaatcggt
      361 cgagatcctc gcatcgggat cggccagcag cttgaaactg agtccttcct tcgcgcaaaa
      421 atctttgtgc gattgcgccg tatcgacgct cacgccgaga accaccgcgc cggcttcttc
      481 gtacttggcc aggtcgcgct ggaaattcct cgcttccatc gtgcacccgc tcgtgaaatc
      541 tttcggataa aaataaagga ccacccattt gtcacgataa tctttcagat tgatctggct
      601 gccatcgctg gttgttaaac taaaatctgg cgctggcgtt ccggccgcgg gttgcttttg
      661 tgtctcggct gctggcacga agaccaatgt agtgagcata acaatcgctg ataagaatat
      721 tttgttcatt ttcgcatctc ctgatttcct gacgaaccta ccaataatcg aattgcgatc
      781 aaaaggaaca tgatctcgcg gatctggacg atcggacatt cgacgcggac gatcgacgtc
      841 ttcatttcgt tgctagaaga gaacggaatc aagttggttg ccgatgtccg aagcttgccg
      901 ggatcgaggc gttatccgca attcaataaa gaaactttgg ccgaatcgct gagcgagcac
      961 cggattcggt acgaacattt cccggagctg ggcggccggc gcagagcaaa gaaggattcg
     1021 cacaacacag cctggcgcaa tgcctcattc cggggctacg ccgactacat ggaaacggaa
     1081 gaatttcgca aaggcaccga acgtcttctc gatcttgcga gcaagctggg tcccaccgcg
     1141 atcatgtgcg cggaagcggt ctggtggcgt tgccatcgtg cgctgatttc cgattacctg
     1201 aaagcgcaag gggctgagat catacacatt ctcgatgcaa acaagagaga gctgcatcca
     1261 tacacatccg cggcgcgcat catcgacggc ggcctaagtt atgcgagcga ggcggatggt
     1321 ttaatttagc gtgaatcaga cgcggttgat ccatcgctag ctaaacgccg gcgtggttcg
     1381 cgccatgtgc ggtgtgcgat ataggccggc accggcggcg cgagcttctc gcgttccatc
     1441 attctttcag ccagccagtg aaaccattcg gagcctgtct cgcggttttg ctcgcgacgc
     1501 cattgctcgg cccaaacgtg aagcttcctc caggagagaa ggatgggtcc gctgaaaaag
     1561 tcgtcgacca gatctagtgc aagctcgcga cggaacacca acacgcctag gctttcccaa
     1621 gtcaatgaaa ccaggtaaac cgcgtcttca ccgtcaggtc cgagcacttc ccgtattttt
     1681 tgtgcgtccg ctccatctgg caacaacagc acgcgacgaa gcgcactggt aaaagccggg
     1741 ctttggaatg atctgaccaa ctcgagcatc gcatctcgtt ttctccgctg gcgataatac
     1801 ctgatttgcg cggccgcaaa gatgacgccc gccgtcaccg cgaatgcgtt gattaggttt
     1861 gcaagggttg agatgtccac gtcagcgagg atcggttaca catccgagga aaaggcatac
     1921 gccgctggga acatgctgaa tcgcgtaaac gaatgggcga tcgactttca cttcaatcgg
     1981 cggtggcgga ggcgatctca gagcggtccc ggccatcatg gccacggccg tggcggcggc
     2041 ggcttccgtt cccttttcgt ccaccgcgat aaaggttttg tgaaaaatct gcgagatata
     2101 gaggtaatcc ctcggtgttc gcggcgccat tcgatcgaaa ttggcgcttc cccgcggctg
     2161 atcgaatgcg gttttcattc cgagcgcctc gaattgtttt gctaacgcta tcgtcggcgg
     2221 ctcgattttg aactttggca gatgcagatc gatctcgcgc atttcgagct ttgcgcattg
     2281 cgtcagcatc tcggcggtca gtttcgattc aagcgctttc agaccatttg cgtcatcggg
     2341 aagaagaacg aggaactgga gttcattgcc cgtgtaggga agactcactg cggcgaatcc
     2401 gtcgcgcttc gcataaccaa aatgccgatc cagttttcgc atcatcggaa catcgacagg
     2461 cgtttcgccg cgaacgtgaa acggctccgg cttcgttgtc ttttccgaga acggatcggc
     2521 ccacgacgcc ttgagataaa gcgcgttcgc caggacaagt cgtgtcgtct cattcagtgc
     2581 gctcgcggga atgagatcgc gaatccggtt acgggtttga tcagcgaccc atttattgat
     2641 gtgctgcgtg gctccggagg cgtcggcaat gaaatcgatt ggctcaaatg ccgccccgta
     2701 attttgtttc acaagcgaca gaaacgactc gcgcaagtcg tacccctttt gggcaaagag
     2761 ccgattggcg atactcagcg taatcggttc gctcgggccg ccgaatcttt tcgcttgttt
     2821 tacgagttcg gccgtctttg cgctcatttc ttcgagcgaa tgctggagcg aagcaaacga
     2881 ggccggcact gcgtcgccat cgtttggaaa atgcaaaacg cgcgccatct cgctgcgggt
     2941 ctcgccgtcc gcgccggcga acgtcatcgc cagcgcgctt tcgatcgaat aaggcgaaac
     3001 gcaaagattc tcgttgtctg tggcgagctg gcggtgcaga tcgacagcga gttggtttgt
     3061 ggcttttgcc gcgagatcga agcttgttgc ggcgtgcgcc atcgcaccca ttaacgcgct
     3121 aaacacctgc aagatcaaaa atcgtttcat ttgaatcatg aaacaccgaa atcgcgaagg
     3181 ttgcgacgtg aaaagactga aagacctatc gttccttttt taggggatac tcctgtcatc
     3241 tcgaccgaag tggagagatc t
//
LOCUS       DASQUQ010000052         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_279224, whole genome shotgun sequence.
ACCESSION   DASQUQ010000052 DASQUQ010000000
VERSION     DASQUQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279224"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VE486_01400"
     CDS             <1..379
                     /locus_tag="VE486_01400"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HZA37775.1"
                     /translation="WIAIPIVTIVFLAIFGTPILIVAVILYFSFSKTRALHRTVRLMV
                     EKGQPVPEALLNPPPAQRQRSDMRRGVVLVMVGLGLMLFLAAVNDWEGGAWSIGLIPF
                     LIGAGYLLVWKLEGRKDNVPPVP"
     gene            389..925
                     /locus_tag="VE486_01405"
     CDS             389..925
                     /locus_tag="VE486_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZA37776.1"
                     /translation="MELTDADLVARVLVDDDQHAFGELVRRHQSSVRGLLRQLTRADI
                     ALADDLSQETFLRAYKNIRGFRGEARFSTWLYRIAYNCFRENARRRKELVGIDEEQLQ
                     SEHDPQVADPALRHDLMYALSLLPLHERSAIVLCCQNGLSHEEAARVLDIPLGTVKTN
                     VLRGREKLKRTLVAWGPD"
     gene            927..1268
                     /locus_tag="VE486_01410"
     CDS             927..1268
                     /locus_tag="VE486_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37777.1"
                     /translation="MDEKPQEDWLDRQLREAAPYIDDEGFAARVLRQLPPPRRGRDSL
                     RAVILLGISLLASALVYVLSDGGRFLLVAMARLSALPTFWLLALAFGAGLLLMVGGVV
                     AAIARTNESRP"
     gene            complement(1488..2417)
                     /locus_tag="VE486_01415"
     CDS             complement(1488..2417)
                     /locus_tag="VE486_01415"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZA37778.1"
                     /translation="METLHSISELARLSGPLFLAIGVFDGVHRGHQAVISTSARHAQS
                     ENGTPVVVTFDPHPMKVLRPHDAPHLLTATQHKIALIRDLGVQHLLVITFNREFAATS
                     PENFVEQLVAHSKPLREICVGHEWSFGKDRRGNLDLLKKLGAQYFFDVVGIPPVKVNG
                     AVVSSTAIRQAVEKGDLAKAAAMLGREYTILGTVVSGDKLGKKLGYPTANLSAHSEQF
                     PPNGVYFAEATLDGAVYPGVVNLGYRPTVSIGKSERILEIHLLDFEGDIYGKDIEVRF
                     MRYLRPEQKFGSLEALVRQIELDVRQARELCAS"
     gene            complement(2589..>2997)
                     /locus_tag="VE486_01420"
     CDS             complement(2589..>2997)
                     /locus_tag="VE486_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37779.1"
                     /translation="RAAFEKFRGDFYQMPPMVSAKKHGGVPLYKLARQGKVVEREPRL
                     VHVYRYSIDRIALPEIDFSVLCSKGFYVRTYVHDIGEELGCGAHLKSLRRTKSGRFDV
                     AQAISVDQIKNAPREEILSRLLTLPEVSRLRGA"
BASE COUNT          631 a          814 c          866 g          686 t
ORIGIN      
        1 ttggattgcg ataccaatcg tgacgatcgt ttttctcgcc atcttcggga cgccgatcct
       61 catcgtcgct gtgatcctgt atttcagctt ctcgaaaacc cgggcgttgc atcgcacggt
      121 tcgtttaatg gtggaaaaag ggcagccagt gcccgaggcc ctgctgaatc cgccgcctgc
      181 acagcggcaa cgctctgaca tgcgtcgtgg tgtagtcctg gtgatggttg gccttggtct
      241 gatgctcttc ctcgccgccg tcaatgattg ggagggcggt gcgtggagca tcggccttat
      301 tccctttctg attggcgcgg gctatctcct ggtatggaag ctggaaggaa ggaaggacaa
      361 tgtgccgccg gtgccgtaat ctgcggccgt ggagctgacc gatgcagacc tcgtcgcgag
      421 agttcttgtc gatgacgatc agcacgcatt cggcgagctg gttcgccggc accagtccag
      481 tgtgcgaggt ctgctgcgtc aactcacccg agcagatatc gcgcttgcag acgatctctc
      541 gcaggaaact ttcctgcgcg catataaaaa catccgcggc tttcgcggtg aagcccggtt
      601 ttcgacatgg ctttatcgca ttgcttacaa ttgttttcgg gaaaacgcgc gccgtcgcaa
      661 ggaactcgtc ggaattgatg aagaacaact gcaatccgag cacgatcccc aggtggccga
      721 tcctgcttta agacacgatc ttatgtatgc gttgagtctg cttcctttac acgagcgttc
      781 ggcaattgtg ctctgctgcc agaatggcct ttcgcacgag gaagctgcgc gggtattgga
      841 tattccgctt ggcacggtga agacgaacgt gctaagagga cgagagaaat tgaaacgaac
      901 gctggtagca tggggccctg attaacatgg acgaaaaacc acaggaagat tggctggatc
      961 gacaattgcg cgaggccgcc ccgtatatcg atgatgaggg atttgccgca cgcgtgttac
     1021 ggcaattgcc tccgccgcgg cggggccggg attcgttgcg ggctgtcatt cttcttggaa
     1081 tcagcctgct cgcgagcgcg ctggtatatg tattgtcgga cggaggacgt tttcttctgg
     1141 tagcaatggc acggttgagc gcattgccta ctttctggct tcttgcttta gcgtttggcg
     1201 ctggcctctt acttatggtc ggcggcgtcg ttgccgccat cgccaggaca aacgagtcgc
     1261 gaccttagat atcgccgcgg gtggcagtgg atctacttgc tcaacccacg cacgcggcac
     1321 aattgggcgt ccacgcagtt gcagcgcggt ttgggtaaat ttgtcatgtc tggcgcagtc
     1381 gagacatctc tcgatgttct tcccgtgcga cgacccagag taacagtaag agattcctcg
     1441 actccgctcg gaatgacaga ggagtcggtt ctttgttgag gcatgcttta actggcgcaa
     1501 agttcgcgcg cctggcgaac atcgagctcg atctgccgga cgagcgcttc caggctgccg
     1561 aacttttgtt ccggcctgag ataccgcata aagcggactt cgatgtcttt gccgtaaatg
     1621 tcgccctcga aatcgagcag atgaatctca agaatgcgct ccgatttgcc aatgctcacg
     1681 gttggccggt aaccgagatt caccacaccg ggatataccg cgccatcgag ggttgcttcg
     1741 gcgaaataga ctccattcgg cggaaattgc tcgctgtgtg cgctcaggtt ggccgtaggg
     1801 taaccgagtt tctttccaag cttatcgccc gacacgactg ttcccagaat cgtgtactca
     1861 cggccaagca tcgccgcggc ctttgccaga tcgcctttct ccaccgcctg ccgaatggca
     1921 gtgctgctaa caaccgcgcc attcactttc accggcggaa tgccaacgac gtcgaaaaaa
     1981 tattgcgcac caagtttctt gagaagatcg agattgccac gccgatcctt tccaaatgac
     2041 cactcgtgtc ctacgcaaat ttcgcgcagc ggcttcgaat gggcgaccag ttgctcgaca
     2101 aaattctctg gagaggtggc ggcgaattct ctgttgaagg taataacgag aaggtgctgg
     2161 acacccaaat cgcggatcaa cgcgattttg tgctgagtgg cagtgagcag atgcggtgcg
     2221 tcatgcggcc gcagcacctt cattgggtgc ggatcgaacg tcacgaccac aggagtcccg
     2281 ttttcggact gagcgtggcg cgctgacgtg gaaataaccg cttgatgacc gcgatggacg
     2341 ccatcgaaaa cgccgatcgc gagaaagagc ggaccggaca aacgcgctaa ctcggagatg
     2401 gaatggagcg tttccatcag tcgaatgacg aatgaaggcg aaatgacgaa tgacccgcct
     2461 ccgccaggct tcggcgtggc tgacgaatga cgaatgacga atgaaggcga aatgacgaat
     2521 gacgaagaat tagtgcttcg ggcttcgtca ttccttcgtc cttcgtcatt tgagcttcgt
     2581 catttttttc acgccccgcg caggcgcgaa acttccggca gtgtcagcag gcggctgaga
     2641 atctcttcgc gcggcgcgtt tttgatctga tcaacgctga tcgcctgagc gacatcgaaa
     2701 cgaccggact ttgtgcgccg cagcgatttc aagtgcgcgc cgcaaccgag ctcctcgccg
     2761 atgtcatgca cgtaggtgcg aacgtaaaaa cccttgctgc aaaggacgct gaaatcgatt
     2821 tccggcagcg cgattcgatc gatgctgtaa cgatagacgt gaacgaggcg cggctcgcgc
     2881 tcgaccactt ttccctggcg cgccagttta tagagcggca caccgccatg ttttttcgct
     2941 gagaccatgg gcggcatctg ataaaaatcg ccgcgaaatt tttcaaaggc ggcgcga
//
LOCUS       DASQUQ010000053         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_642235, whole genome shotgun sequence.
ACCESSION   DASQUQ010000053 DASQUQ010000000
VERSION     DASQUQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642235"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1075)
                     /locus_tag="VE486_01425"
     CDS             complement(44..1075)
                     /locus_tag="VE486_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZA37780.1"
                     /translation="MLETATRSKKSRSSKPAPHERFLEAFRWMLLTRTLEEKLVSLYR
                     GGLITGGVYIGKGQEAVSVACGLCLQKGDIFAPLIRDQAGRSAFGEPLLDVTRTYLGS
                     RLGPMRGRDGNIHRGRPQENQLAMVSHLGAMISVTVGALMAKRFKGEKNFVGLACIGE
                     GGMQAGSFHEGMNIAAVEQVPLVVVATNNHYAYSTPNDREFACHDLVDRAIGYGFEGY
                     SLDGTDLSACLDVIGGAVKRARAGRPPQLVVASVLRLSGHGEHDDASYVTEEIRREPF
                     ARDCLKVAEQTITDLNLLDAETLAEWRRDAAAQVDEAIATAQKEAPPEADKEDWCAIS
                     TRELADSLE"
     gene            complement(1316..>3533)
                     /locus_tag="VE486_01430"
     CDS             complement(1316..>3533)
                     /locus_tag="VE486_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37781.1"
                     /translation="AQGSQSVLKGLDNPSRIQPGLAGALVSNLDVDPEFIAAIEAALG
                     RNLQAIVLKNSQLAPEIIAKLKEKKIGQAALVLPSLSNSSPETHAALPQGALAWAREK
                     VKAPNPLAPLMARLLHNVAIFCDLDAALSFKKQGSDFAAATLAGEFISAEGIVFGGSR
                     DASADSLLERKARISVLNAEYAEVRRQRDALLQKRDETNAILEKAAGEFEEARRQFAS
                     ADRDQSACQNRILFLEQESQGGEQKLEQLSSEQTVLAQQIQTADERIAKLGEELGSER
                     AALEAQQNEQLKLQGAREKAAKSEDEAREQLNELRLAFATERERYENLVAQRQPMTAR
                     EAELAETIAVRQAEIANFEKRLVAQSEESKSAEAAIEKEKAECAKLEATLATLTDQRA
                     ESSRVMNGIDSDLRARRNSLNELHDLRSKQQVRESQLQMRSDNLVEHISRRYQLNLRE
                     FAPDQVAFEKTLSAQIKRNEKLEDGAPAMPGSRELAPPDVEKLIAELNRRLDNMGPVN
                     LDAVHEYDELEGRYRFLETQNNDLTNSRRELLDVIAQINSTTRKLFAETFAKVRVNFR
                     EMFAELFGGGRADLSLLDENDPLNCGIEISAKPPGKQLQSVSLLSGGERAMTAVALLF
                     AIYMVRPSPFCILDEVDAPLDEGNINRFIGVLERFVKHSQFIIMTHNKRTIAKADVLY
                     GVTMEERGVSKLVGMKLTTPPAGEQTATTRKAVSSQREFAMAGNGGTEANSSFAAR"
BASE COUNT          682 a         1029 c          964 g          858 t
ORIGIN      
        1 aattttgcct gcgcttcgcg gatcgcttcc aaataggtga tgctcattcg agggaatcgg
       61 cgagttcgcg cgtggagatc gcgcaccaat cttctttgtc ggcttcgggc ggggcctctt
      121 tttgcgcagt ggcaatcgcc tcatccactt gggcggctgc gtctctgcgc cattcggcca
      181 gggtttcggc atctaaaaga ttaagatcgg taatcgtttg ctcggcgacc ttcaaacaat
      241 cgcgcgcaaa cggttcgcgc ctgatttcct cggtcacgta gcttgcatcg tcgtgttcgc
      301 cgtgaccgga caagcgcagg acggaagcga caaccagctg cggtggacgc cccgcccgcg
      361 cccgttttac agcgccgccg atcacgtcca ggcacgcgct cagatccgtt ccatcgagac
      421 tgtaaccttc gaatccgtaa ccgatcgcgc gatcgaccag atcatgacac gcaaattccc
      481 ggtcgttagg cgtggaatag gcgtaatgat tatttgtcgc gacaaccacg agaggcactt
      541 gttcgacagc ggcgatattc attccttcgt ggaacgaacc ggcctgcatc ccgccttcgc
      601 cgatgcaggc gaggccgacg aagtttttct cccccttaaa tcgtttggcc atcaaagcgc
      661 ccaccgtgac cgaaatcatc gcgccgaggt gactgaccat ggcgagctgg ttctcctgcg
      721 gccggccccg atgaatattg ccgtcacgcc cgcgcatcgg gccaagacgc gagccgagat
      781 aagtgcgggt cacgtccagc agcggttcgc cgaatgcgga tcgccccgcc tggtcgcgaa
      841 tcaacggcgc gaagatgtcg cctttctgca aacacaagcc gcaagccacg ctgaccgcct
      901 cctgaccttt tccaatgtaa acgccaccgg tgatcagacc gccgcgataa aggctgacga
      961 gcttttcctc gagggtgcgc gtgagcagca tccaacgaaa tgcttcgaga aagcgttcat
     1021 gcggcgcggg cttcgaagac ctggattttt tgctgcgcgt cgccgtttca agcatccgcc
     1081 aattctacgc aagatcgaca aatacgcgca aaattttttt gatggcggat cgtgagaaca
     1141 atctttttct ggggagcgca ggctgccagc ctgtcctttt cggcagcttg cggaaaagct
     1201 atttgaacct ttgacccgag tggttcatgg aacgccgatg tcgtcggcaa gctgccgacg
     1261 actacaggct ggcagcctgt gctccccgga atttaatcac gcgagaagtg cgattttacc
     1321 gcgcggcaaa gctggagttc gcttcggtac cgccgttccc ggccatggcg aattcccgtt
     1381 gcgaagaaac tgctttgcgc gtagtcgccg tctgctcacc ggcgggcggc gtcgttaatt
     1441 tcattccaac cagtttgctc acgccgcgct cttccattgt gacaccgtag agcacatccg
     1501 cttttgcaat cgtgcgtttg ttgtgggtca tgatgatgaa ctggctgtgc tttacgaatc
     1561 gctccaggac gccgataaag cggttgatat tgccttcatc gagcggcgca tccacttcgt
     1621 cgagaatgca gaatgggctc ggtcgcacca tgtaaattgc gaacagcaac gcgacggctg
     1681 tcatggcgcg ttcgccgccg ctcaacaacg agacgctttg tagttgtttg ccgggcggtt
     1741 ttgcgctgat ctcaatccca cagttgagcg gatcattctc atcgagcaac gacaagtcgg
     1801 cgcgacctcc gccgaagagc tcggcaaaca tttcgcggaa gttcacgcgc acttttgcga
     1861 acgtttcagc gaacaacttg cgcgtagttg agttgatttg cgcaatcaca tcgagcaatt
     1921 cgcggcgcga attggtcaaa tcgttgtttt gagtttcgag aaagcgatat cgcccttcga
     1981 gctcatcgta ctcgtgcacg gcatcgagat tgaccgggcc catgttgtcg agccgtcggt
     2041 tgagctcggc aatgagtttc tccacgtctg gaggggcgag ctcccgcgag cccggcatgg
     2101 caggagctcc gtcttccagt ttttcgttgc gtttaatttg tgcgctcaag gttttctcga
     2161 acgcgacctg atccggcgca aactcgcgaa gatttagctg gtaacgccgg gagatatgct
     2221 cgaccagatt gtcgctccgc atctgcaact gcgactcgcg aacctgctgt ttcgaacgca
     2281 aatcatgcag ctcattcaac gaatttcgcc ttgcgcgaag gtccgaatct attccgttca
     2341 ttacgcgtga gctttccgca cgctgatcgg tcaatgtggc aagagtggcc tccagtttcg
     2401 cgcattctgc tttctctttc tcgatggcgg cttcagcgct ctttgattcc tccgattggg
     2461 cgaccaatcg cttttcgaaa ttggcgatct ccgcctgtcg aaccgcgatt gtttcggcga
     2521 gttccgcctc gcgagcagtc atcggctggc gctgtgcaac gaggttctcg tagcgctctc
     2581 gctcggtagc gaacgcaagg cgcagttcat tgagctgttc cctcgcctcg tcttcactct
     2641 ttgccgcttt ctcgcgtgca ccctgaagtt tgagctgctc attttgctgc gcctccaaag
     2701 cggcgcgctc tgagcccagt tcttccccta attttgcaat ccgctcgtcg gcggtttgga
     2761 tttgctgcgc gaggactgtc tgctccgagc taagttgctc gagcttctgt tcacctccct
     2821 gcgattcctg ttccaggaag agaattcgat tctggcaggc ggattgatcc cggtcggcag
     2881 acgcaaattg acggcgagct tcttcgaact cgcctgccgc tttttctaat attgcatttg
     2941 tctcatcgcg tttttgcagc aatgcatcgc gctgcctgcg tacctccgcg tattctgcgt
     3001 tcagaaccga tatccgcgct ttgcgttcga gcaaagaatc ggcagacgcg tcccggctgc
     3061 cgccaaaaac tattccttcc gctgaaatga attctcccgc gagcgtcgct gcggcgaaat
     3121 cgcttccttg ttttttgaaa gagagtgccg cgtccagatc gcaaaaaatt gcgacattgt
     3181 gcagtaaccg cgccatgagc ggcgcaagag gattcggcgc tttcactttt tcgcgcgccc
     3241 aggcgagggc tccttgcgga agggctgcgt gagtttcagg cgaagaattc gacaagctcg
     3301 ggagaaccag tgccgcctgc ccgattttct tttcctttaa tttggcgatg atttccggtg
     3361 ccagctgcga gtttttcaga acaatcgctt gtagatttcg acccagggca gcttcgattg
     3421 ccgcgataaa ttctggatcg acatccaggt tcgaaaccaa agctcccgcc agaccgggtt
     3481 ggatccgtga cggattatca aggcctttga gcacagattg cgaaccttga gcg
//
LOCUS       DASQUQ010000054         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_99103, whole genome shotgun sequence.
ACCESSION   DASQUQ010000054 DASQUQ010000000
VERSION     DASQUQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99103"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..157
                     /locus_tag="VE486_01435"
     tRNA            76..157
                     /locus_tag="VE486_01435"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:110..112,aa:Leu,seq:cag)
     gene            727..1419
                     /gene="ubiE"
                     /locus_tag="VE486_01440"
     CDS             727..1419
                     /gene="ubiE"
                     /locus_tag="VE486_01440"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HZA37782.1"
                     /translation="MTKPSIETQPERVREMFGSIAARYDLANHLLSCGIDFYWRRRAA
                     EIVANWRPDKIVDLASGTGDLALALQKRLPDAEIVGVDFLPEMLELAKRKGVRQTVLA
                     DAMKLPFANASFDCVTVAFGLRNMEDWGEALGEMSRVLKGNGHLLVLEFSLPTMPILR
                     AVYRFYLHRCLPLLGSFLTRKKSAYGYLGDSIEDFPSGNAMCQLMAATGFHDANAQPL
                     SGGIVTIYTAEK"
     gene            complement(1481..2719)
                     /locus_tag="VE486_01445"
     CDS             complement(1481..2719)
                     /locus_tag="VE486_01445"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37783.1"
                     /translation="MPTANVVIFVPHAKKSPMPKAKTKAASGNVHAVVGSDEGEVKRV
                     AAELAANLTPPDAGDFGLETIDGVAENAEQAAARIRSAIEALQTLPFFGSSKVVWLKN
                     ANFLGDTQIGRAAGVQAALEELSDLIENGIGSDVIFLINATEVDKRRSFYRSLAKRAE
                     LQVFDRLDSSRSGWEEEAIEVIRRRAKKRQLQFDEDALDLFVLLTGGDTRQIENELEK
                     IDIFLESGTGSLPVGQAGLSPARLEPAARMAVPRVTVDLVRELVPLSRAGVIFELSNA
                     LATRDLELALKLVRRLLDQGENAVGILLVAILPTVRNLLLTKDLMEHHRLARPHSPFQ
                     FISAINRLPAEATDHLPRKKDGSINAYPLGIAAQHAHRFETKQLVRAMQACLEANLQL
                     VTTQLDHELILTEAVVKSLG"
     gene            complement(2766..>3449)
                     /locus_tag="VE486_01450"
     CDS             complement(2766..>3449)
                     /locus_tag="VE486_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZA37784.1"
                     /translation="KRTAEDLADYLRDVGLKVRYLHSDIDTIERVEILRGLRAADFDI
                     LVGINLLREGLDLPEVSLVCILDADKEGFLRSQTSLIQTAGRAARHIHGEVVLFADTV
                     TQSMEALMSISDYRRTKQMEYNEKHGITPQTVRRAVQESLHTILRGREIAASVIQEAG
                     GDFNVTELLRELEDDMQRASANLEFERAALLRDQIMEVKSGAGIDKIEPKRRPVKYSR
                     NAGRRRSRV"
BASE COUNT          709 a          968 c          950 g          822 t
ORIGIN      
        1 gcccttacaa cccaccgcta taacgcactt gcgcgtctaa gggcccgtgc gcattttatg
       61 acccgctttc tgattgctca cgtggcggaa ttggcagacg cgtacggctc aggaccgtat
      121 ggggcaaccc gtggaggttc gagtcctctc gcgagcaccg tctggcttct atcgcgctgg
      181 tccggttaac aagcctctta gcaaaggccc aaggaaggcg ggataccagg tcaatcggcc
      241 ggtttacttg cttgcggctt ttcttcttgc cgcccaccgg gccttcatca actgggaaag
      301 tcgtctgcgc ccggcagcgg taattccacc ttttttcctg gcgggcgttg tttttgtttg
      361 tccttttatc cttgcccagc gcgctctggc cgcggccgca agtttggcac gagtcgcggc
      421 cggcatccgg cgacggcccg cgccgccgct tacagccctc gcaggcgaac ctgacaggat
      481 tgaggaaaga cgtttttgta gagtatcgat ttgccggcga atcgagacgg cttcttgtag
      541 agcttcaaca gatatgtcca tgccttgtca catagccgta gttggctgga tggcaatcaa
      601 cttttttaac aaaccagata ttcgaagttc ttctccttac tccagcctgc tattaccttt
      661 tgcgataatg aaggaaagga gcaagcggtc acgaagttgt gagttgattt ttgaagctat
      721 ttcaaaatga ctaagcccag tatcgaaaca caacctgagc gagtccggga aatgttcgga
      781 tccatcgccg cgcgctatga tctggcaaat catctgctca gttgcgggat cgatttttat
      841 tggcgaagac gcgccgctga aattgtcgca aactggcgac cggataagat tgtcgatctc
      901 gcttccggca ccggagattt ggcgctcgca ttgcaaaaga gattgccgga tgcggaaatt
      961 gtcggggtcg atttcctgcc ggagatgctg gaactggcga aacgaaaagg tgtgcgccag
     1021 accgttcttg ccgatgcgat gaagcttcca ttcgccaatg cttcattcga ttgtgttacg
     1081 gtggcattcg gtttgcgtaa catggaggac tggggagagg cgctcggcga gatgtcacgc
     1141 gtgcttaagg gcaatggcca cttgcttgtc cttgagttct cactgccgac gatgcccatc
     1201 ttgcgcgcgg tctatcgatt ctatttgcat cgctgcctgc cattgctcgg atcctttctc
     1261 acaaggaaga agagcgctta cggttatctg ggtgactcga ttgaagactt cccgagcggc
     1321 aatgcgatgt gccaactgat ggcagcaacc ggcttccatg atgctaacgc tcaaccgctc
     1381 agcggcggca tcgttacaat ctatacggcg gagaaatagt agtggcggtc tatgtcgtcg
     1441 ctcaaacaaa tgcgatgctt acagagcgcc ggcacagcga tcagccaagc gacttcacta
     1501 cggcttcggt taagatcagc tcgtgatcca gttgcgtcgt gacaagctga aggtttgcct
     1561 caagacacgc ttgcatcgcc ctgaccaatt gcttggtttc gaaccggtgc gcatgctgcg
     1621 cggcgattcc cagagggtag gcgttaatcg agccatcctt ttttcgcggt aaatggtcag
     1681 ttgcctcggc gggcaaacga ttgatcgccg agataaattg gaacggcgaa tgcgggcgcg
     1741 cgaggcggtg atgttccatc aagtctttgg tgagtaacaa attccggact gttggcagaa
     1801 ttgcgaccag caaaatacca acggcgtttt cgccttgatc caataaccgg cgcacgagct
     1861 taagagcgag ctccagatcg cgtgtcgcca gtgcgttgct caactcgaaa atgacgcccg
     1921 cgcgcgaaag aggcaccagc tctcgaacaa gatcgacagt gacccgtggc acagccatcc
     1981 tggctgccgg ttcgagcctg gcaggcgaga ggcccgcttg ccccacaggc aggctgcctg
     2041 tgccactttc cagaaagatg tctatcttct ccagttcgtt ctcgatctgg cgggtgtcgc
     2101 caccggtaag tagaacaaac agatcgagcg catcctcgtc gaattgcagt tgtctctttt
     2161 tcgcgcgacg ccgaatcact tcgatcgctt cctcttccca gccgctccga ctcgaatcga
     2221 gccgatcgaa aacctgcagc tcggcgcgtt tggcgagcga tctgtaaaac gaacgacgct
     2281 tgtccacttc cgtcgcgttg atcaaaaaga tgacgtcaga accgattcca ttctcgatta
     2341 ggtctgataa ttcctcgagc gcagcttgaa cgccggcggc gcgtccgatc tgggtgtcac
     2401 cgaggaagtt cgcgttcttt aaccacacga ctttggagct gccaaagaaa ggcaaagttt
     2461 gcagcgcctc gattgccgat cttatccgcg ctgccgcttg ttccgcgttt tcggccacgc
     2521 catctattgt ttccaatccg aagtcgccag cgtccggtgg tgtcagattg gccgcgagct
     2581 cggcagccac acgtttcact tccccttcat cggacccgac caccgcgtgg acgttgccgc
     2641 ttgccgcttt tgttttcgcc tttggcattg gagatttctt cgcatgcggg acgaagatta
     2701 caacattggc cgttggcatg ctgatccggg gagcaacagg ctgccagcct gtgctcccca
     2761 gcaatttaaa cgcgtgagcg gcggcgaccg gcgttgcgcg agtatttcac cggtctgcgt
     2821 ttcggttcga tcttgtcgat accggcaccg cttttcactt ccatgatttg atcccgcaaa
     2881 agcgccgcgc gctcgaattc gagattggcg gaagcgcgct gcatatcgtc ttcgagctcg
     2941 cgcaacaact cggtcacgtt gaaatcgccg ccggcttcct gaatgaccga ggcagcgatt
     3001 tcgcgtccgc gcagaattgt atgcaaactt tcctgcacgg cccgccggac ggtttgtggc
     3061 gtgatgccat gcttttcgtt gtattccatt tgtttggtcc ggcggtagtc cgagatcgac
     3121 atgagcgctt ccatgctttg ggtgacggta tcggcgaaga gcacaacttc gccatgtata
     3181 tgccgcgccg cgcggccggc ggtttggata agggaagtct gactgcgcaa aaatccttcc
     3241 ttgtccgcgt ccagaatgca gaccagcgaa acttcgggaa gatcgagacc ttcgcgcaac
     3301 agattgatcc cgaccagaat gtcaaaatcg gcggcgcgca atccgcgcaa aatttctacg
     3361 cgctcgatcg tatcgatatc gctatgcaaa taccgcacct tcagtcccac atcgcgcaag
     3421 taatcagcaa ggtcttcggc ggtgcgttt
//
LOCUS       DASQUQ010000055        21119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_279730, whole genome shotgun sequence.
ACCESSION   DASQUQ010000055 DASQUQ010000000
VERSION     DASQUQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 21119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21119
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279730"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VE486_01455"
     CDS             complement(<1..287)
                     /locus_tag="VE486_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37785.1"
                     /translation="MQGSLGHIIWTICYLSVLAGLSVYGIHRYFIIYLYLKNRRRPPV
                     PAGKFEQLPKVTVQLPIFNEVYVAERLLRSVSELDYPQHLLQIQILDDSTD"
     gene            549..3053
                     /locus_tag="VE486_01460"
     CDS             549..3053
                     /locus_tag="VE486_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZA37786.1"
                     /translation="MQLRDTFKKRRRDRFERLKIILPILLAGWIPSILTLIVSYTILR
                     DTLETRILRDRQTLIQLVGHLVGHDLSRSSSVIEYYQTLPAVAEVLSAPIPEPFAQQW
                     LNAAFYSHPQIDGMFITASDGRLIGSIPTDPEIIGKDFDSNQWRDQAAASNEAFVSAV
                     HPRLQDKRLATDIVGAVRAPDRTILGYVGVSVLVERMGRRLSTIEFTDRLLFGVLDQN
                     GNSLFARDFKPNPNAISPESGILLKETRQSTSGHFEREGNLYSFSPIESTGWVAVVEQ
                     PRSLAYKPVHDLLGRMTILAVWLILLTAVFAWLGTRFYQRQMESTRRIEREVVFNEKI
                     LANMPSGIAFIDPKSHHFLQANDAFAHMAQRFGDLPPGRDITEATCDDVKIAPAGAIE
                     RVLSFGTPFQLIEYPLKDKTGMTHFLDINLLRLQGSHETIQGVLYLVEDKTRDVKLRQ
                     ELIDANAAKDQFLALLSHELRNPLSPVIAMVGELEATIPDSPTVREALEVIRRNVELE
                     ARLIDDLLDVTRIAKGKLQLSFETVCVNQILQRAYEICREDIAAKHLEVEFRLRATDT
                     HVEGDPARLQQVFWNLIKNSVKFTPEKGRITIETINPAPDKIDIRIADTGMGIEQERI
                     DKIFNAFEQGQTSITRRFGGLGLGLSISKALIDAHGGKIRVESSGKDQGSTFSLELKT
                     VLSPVSGDGDGARRDRRAALEKDADLPRHRRVLVVDDHQDTCTGMKKMLERRGYQITV
                     AHTAQQAVEKVRTQEFDLLISDIGLPDRSGYELMREVQSTKALPGIALSGFGTEQDVN
                     EALAAGFSGHLTKPINFERLEEAISNLLEPEPTCHS"
     gene            3106..3885
                     /locus_tag="VE486_01465"
     CDS             3106..3885
                     /locus_tag="VE486_01465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZA37787.1"
                     /translation="MPTKNASAYQPLDWDFGFLAENAAVRSKRFNMTAEAKSVRYPIR
                     LLRYWFAYHLLRIESARRAAPIDLVEIGVQKAQFLQFVRSAPVQPNVGRWTVVDAVIH
                     TEKFRKVGIKKEDVLQANLESPDFDLPHAYDAAVLLHVLEHLFEPEAALRKIVRRIRQ
                     GGVLIGGFPVLPHFLVAPHQKRERKTAQPMGHVSVFSPQRVIRMGEAAGCTVEFNSGA
                     FFMRSSGSPLENSAAWLRFNLWWGSRFPSWPGEIYWLMRKR"
     gene            4094..6976
                     /locus_tag="VE486_01470"
     CDS             4094..6976
                     /locus_tag="VE486_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZA37788.1"
                     /translation="MTSAQERKIKQTGCDARFDNLTRQLYSTDASIYQIEPVGAAFPK
                     SAREASAVIHAAADTGLSVIPRGAGTSLVGNAIGEGLIVDFSRYNRQIAELDVEKRSV
                     RVSAGVVLDQLNDFLRPHGFCFGPDVATSSRATLGGMIANNSSGARCPIYGTTADHVI
                     SLEIVMADGRVEKIGPTHESLRGELVKIENLIRARSAEMAERWPPGLIKRWPGYGIER
                     FLRAPNDLTSILAGSEGTLAAIFSAELKIAPLPREKGLGLIFFASVGEAMQATVELLD
                     LQPAAIEHIDRPLLDQTKGQLHFQAARDLLELDSKPCESILIVEFYDDVTDRLSLLQA
                     RKLGLRTKILTDPVQMNLVWSVRKSGLSLLTGCVGPAKPVAFIEDAAVRPAQLPDYVR
                     GLQSIMKPLGLEASYYGHAASGLLHVRPVLDLHSAADLKKFRQVADQTSALVRQFKGS
                     LSAEHGVGIARTEYMRDQLGDELLEVMREIKNAFDPKNIFNPGKIFEVESARCADSRA
                     AGQRSVPTKIDNHLRENFTRPLELPFEPVLAFAFKDRSFVGNLEQCNGCGGCLKHTGI
                     MCPTFMATGDEVMSTRGRANIIRAALELRVNGHDPLKCEEIDAALSNCLSCKGCTPEC
                     PSNVNLALLKAELMYARYRRDGLPLRERIFSNVDLLGRIGCAVPSLANLILDLKPLRA
                     LMEKTVGLSAKRSLPHYANERFDRWFEKRIRRLDGVSPHRQRGNVILWDDTFVRYHEP
                     HIGIAAVKVLEALGFEVALVKNRRCCGRPAFSQGNLGAAAKLAKHNLDLLSSLQNSNT
                     PILFLEPSCWSMFVEDYRELKIQKAEDVAARCFLFEEFVDDLLAQEPDALRFSERPAK
                     VAIHPHCHAKSIMNPAFMKTLAERLGKQATVLDTACCGMAGAFGALAEKYDLSVQVAQ
                     RLLDKINGQPPGTEIIASGTSCRHQIVDLTNLHPKHMAELLAEAIA"
     gene            7171..7611
                     /locus_tag="VE486_01475"
     CDS             7171..7611
                     /locus_tag="VE486_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZA37789.1"
                     /translation="MASPLIRWEPLELEDVQNRLSKLFGRRPNGRDEITLADWAPLAD
                     ITEDDREYVIKTELPDVKKEDVKITVENGVLTISGERKFEKEEKKKKYHRVERAYGSF
                     MRSFSLPDQADASKVKAEFKNGMLTVHLPKSEKAKPKQIEVNVA"
     gene            7727..8158
                     /locus_tag="VE486_01480"
     CDS             7727..8158
                     /locus_tag="VE486_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZA37790.1"
                     /translation="MRASASREGIAEVPSQAIQLFEPPEDAIYTIDATAHLVGVPRRT
                     ILVYCKHQLLSPVINRTDGGYCFDRDGVHALRRIEALRAVCGDELSGIKIILDLTKEL
                     ERLRSVVRFLSQSKWQRQMMPGGKYETPPLGRKSRPHRGRK"
     gene            complement(8344..9078)
                     /locus_tag="VE486_01485"
                     /pseudo
     CDS             complement(8344..9078)
                     /locus_tag="VE486_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
     gene            complement(9235..10026)
                     /locus_tag="VE486_01490"
     CDS             complement(9235..10026)
                     /locus_tag="VE486_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZA37791.1"
                     /translation="MNEETPKPTPPKAFGVGQGMDYGEQADVQQVHAAVQREKREPRV
                     GAEPLSIWLIAVYGLAVFFGGAYLGRYSGNFTGEALDPYGGEPRTMKKITGPPGAEQI
                     AELSPRDRGKKIFAANCQTCHQANGLGVAGQYPPLAGSEFTIGGSRRPAMIVLKGLQG
                     PVKVKGQQYGAAVMQPWEKTLTDQKIADVLTYERSEWGNNASPVTAEQIAALRKELAG
                     RGESFVEHDILAVPDEDLPGGAPAGGESPKPGEAAKPGEPPKPQG"
     gene            complement(10034..11077)
                     /locus_tag="VE486_01495"
     CDS             complement(10034..11077)
                     /locus_tag="VE486_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HZA37792.1"
                     /translation="MKGLAPLFLGIFGTFAFSWVGLTVIPNWQIGYLNPQSDEEGTDI
                     YPMPQSGMVQRGARVYAANGCIYCHSQQVRADYSAADIERKWGDRRSAPRDYIFERPV
                     FLGKMRMGQDLANIGARAPAEEESAQPSGAASPAASPVPQEGAPASSPAPAGPSPSPP
                     GGASPSPGGPPAQAAGSPPPASSPPAAATSPAPNAAPAPETTASPPPAGSPPAQGAAV
                     SSPPSSPSPSAPWPEVTTGEPPMYSAAWHHVHLYSPRSINVDSNMPSYRFLYQRRRIS
                     DAPSAEALHLTGSDAPPEGWEIVPTFDAKCLVAYLMSLNQSHPLKEVRAAAPAAAAGS
                     PSPAAAGSPSPAK"
     gene            complement(11142..12623)
                     /locus_tag="VE486_01500"
     CDS             complement(11142..12623)
                     /locus_tag="VE486_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZA37793.1"
                     /translation="MNATGIPLKEPSVSAAAGDAEQLERALIDASTRVPVLMFYTSAI
                     AWLILGTLLAGFVSFKLHSPDLLSGISFLTWGRVRPAHMNVMVYGWASMAGMGTAIWL
                     MARLCRTVLRFPLLLVAGAGFWNLGVLLGVGGIVLGDSTGYQWLEFPHYAAIILFVAY
                     TLVVSWAVLMFRYRRGEQIYITQWYLLGAFLWFPWLYAAGQLMLFVVPVQGVLQSAVG
                     WWYANNLLFLWFGAIGLGTAYYMIPKVIGRPVYSYHLAAIGFWSYALFSSWTGMQRLV
                     DGPFPAWMITASIAATILTIIPVATVGLNHHMTMQGYFPLMRYSPTLRFTVFGAMAYT
                     VFSVVGVLISLRSVARYVNFSQASIAYSHLGLYAFFTMVIFGSMYYIVPRLIGREWRY
                     ASLIKLHFWASAYGIGLMTLMLLAGGFLQGANMENPALVFSESTETVLPYLRGRSLSG
                     ILLTASHFIFAYHFGLMLLGLGRKSTVPTFLNPVEAEGSEAHL"
     gene            complement(12620..13126)
                     /locus_tag="VE486_01505"
     CDS             complement(12620..13126)
                     /locus_tag="VE486_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37794.1"
                     /translation="MADTESLRQVVHSRAPLSTWLGIVLLFMLFGVIVLAVIGPGPRG
                     SDYEETRAKKRMENLKTAREEAAKALTTYGWIDKNKGVARIPIERAMELTVAELAQQK
                     PAPGGPIATPEQQAPAAQASPAPAGSPQPSATQPAASPSQQAPVSPSPQAQQLPPGSP
                     SPEPAKTP"
     gene            complement(13128..14330)
                     /locus_tag="VE486_01510"
     CDS             complement(13128..14330)
                     /locus_tag="VE486_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37795.1"
                     /translation="MSERSQTVPTPEGEYFESGRFAGLSFVLGLIAVVALALCIVGAF
                     LNPHQFSYSWLFAFAFFFTLCAGCFFWTIVHHATDAEWSVVVRRQLENLGALLVVLAL
                     LFVPILLLRHHLYAWMNIPPGHEALLDSKRAYLNWGFFLGRAVLFLGFFAAAALALRW
                     FSVRQDKDGNPRFTIGMRKAAFISLPMFALCLTFGAFDWLMSLNYTWFSTMFGVYIFA
                     GAAGSSMSLLVLVITALRQAGYLKDVVTREHYHIMGKWMLAFCIFWAYIGFGQYMLIW
                     YANIPEETQFFITRNTESWWALSMLLVIGRFFIPFGVLLLRSIKKEPRRLCIVAGWIV
                     CMQMLDMYIIVLPALHGGGVQVSIWDLVSLIAIGATLGFVYLRLVPRTSLFPVRDPRL
                     IESLELTN"
     gene            complement(14327..15016)
                     /locus_tag="VE486_01515"
     CDS             complement(14327..15016)
                     /locus_tag="VE486_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZA37796.1"
                     /translation="MLRGFLVIFLLCTIAIIAVFGFRGQTSTGPPLELFPDMVRQMKV
                     RAQAPLNLFADGRGPRLPVAGTVPIGYEMPKPGTIEASGPMIGPWSHPHASFSAGTDY
                     YNTGKMGDHWGTGIPLEVTSELMERGRQRFSITCAMCHGAAAEGNGITKQYGLATVVT
                     LQDDRIRKMSDGEIFNTITHGKNTMMAYGSNIIVADRWAIIAYLRALQRSQRAGVADV
                     PEDHRAELEKQ"
     gene            complement(15020..15574)
                     /locus_tag="VE486_01520"
     CDS             complement(15020..15574)
                     /locus_tag="VE486_01520"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HZA37797.1"
                     /translation="MKTPGGHEVYAMAAEYPSAAALYEAAKRVRDAGFKRWDVYSPFP
                     IHGMDEAMGLGKSWLSGWVLFGGVSGLLTAAVVQFGPSWFLYPLDVHGKPWNFFTVPA
                     FFPIMFELTVLFGAFAAFFAMFGMNGLPRWYHPMFNWERFSRVTNDGFFLAIEARDPR
                     FTENGVRELLEQSGGQHITIVHED"
     gene            complement(15571..17019)
                     /gene="nrfD"
                     /locus_tag="VE486_01525"
     CDS             complement(15571..17019)
                     /gene="nrfD"
                     /locus_tag="VE486_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZA37798.1"
                     /translation="MDAAPTAPEMIKETERPLAHVPLVLNRRNFSWLTERISGVVEQP
                     APRWWWVAFTITASVATFGLFCLGFQISTGVGTWGNNIPVGWAWDITNFVFWIGIGHA
                     GTLISAILFLLRQKWRTSINRSAEAMTLFAVICAAIFPGVHVGRVWMAWYLAPVPNNW
                     GIWPNFRSALVWDVFAVSTYFTVSVLFWYTGLIPDLATLRDRATTKIRKFLFGIFACG
                     WRGSNRNWRHYEMAYLLLAGLSTPLVLSVHSVVSFDFATSILPTWHTTIFPPYFVAGA
                     IFGGFAMVLTLLLPARAMFKLQDVITPQHIDKMAKIILLTGSFVAYAYMMEFFVSWYS
                     GNSYERFASWNRVFGPYWWYWWVGMLFCNCLSPQLFWFRWCRRNIFVVYFVCMCVNAG
                     MWFERFIIIVGGLHRDFLPSSWGMFHATWVDIWTYIGTLGIFTSLFLLFVRFLPMIAM
                     SEVKTAVPAADPHYVSPTEQHPAAAGGKERTR"
     gene            complement(17092..20394)
                     /locus_tag="VE486_01530"
     CDS             complement(17092..20394)
                     /locus_tag="VE486_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HZA37799.1"
                     /translation="MKRVFHHPPEPSTGKRYWRSLGELSDTPEFREWLEHEFPAGAAE
                     LKGDEWSRRSFLKLMGASMALAGFGLNGCRRPEAHLVPFTKNVEWTIPGKFLYYATAM
                     ARRTGAIPLIATTVDGRPIKLEGNPLHPASGGATDTFVQASILDLYDPSRSQRFVHKG
                     KKSDRAAFDEYIGKLRNALSTRQGDGLAFLVTETHSPTRERLRAELEKIFPSVRWCVY
                     EPLLSQERSSPVQGLFGDNVRVVPKFDQADVILALDSDFLDCGQGDLASVRAFTSRRR
                     VSAPKDNMNRLYVVENRFTLTGAMADHRLRCPASQIAAFARALAGKILARTNDTSLGS
                     VVANVQLPPGAAKFDEQWLSEAANDLMAKPGASLVLAGPGQPSEVHILAYAINAALKN
                     LGQTLVVRQVPRNPRTIDISQLAADINDGRIKQLFVFEGDPVYNAPRGLVEDNETGQP
                     LDWSDLQKKVPEIVRLGYHEDSTSALSQWHIPAAHYLESWGDALTAEGEYLSIQPMIL
                     PLFGGLSEIELLNALLGGPKTEGPELVQDTFRATNPPGDFDTAWSRLLHDGFASHVQI
                     QDQPPSFNGNAAGLLAQSSALSPPAPSAESPEIVLVGSYAMGDGRYANNGWLQELPDP
                     ITKLTWDNAALMSPGFAKRLSVQTGDLVKIAINEKSARGGRIKRELVIAALVSPGHAD
                     NSITIPLGYARKMPEFSALPYAGGGLKERPGIAEQSGFNGYFLRTAANPYFATADGQA
                     IESVKVTKVGRTYPLSITQEQFSIEGRGLIREATLERYRADNDFAKKGPGQEELHHRL
                     PTIYSHPPLDAPQQWGMTVDLNVCTGCSACMIACQSENNIPIVGKLQVAHGRVMHWIR
                     IDRYYASRKPFNQDRGDWPEDPEIVHEPMMCQHCENAPCETVCPVNATLHSEDGLNVM
                     AYNRCIGTRYCSNNCPFKVRRFNYFDYNQRPVGKRKIAGALSIYEEYFAPLTTKGAPD
                     IIKMQKNPNVTVRMRGVMEKCTFCVQRIEEAKIAAQARAGASPDTLIPRDSFTTACAQ
                     ACPTEAIVFGNIKDPESRVSKMKGQDRNYRLLEYLNVNTRTSYLARIRNPNPKMPDAA
                     NIGVASLEEKPG"
     gene            complement(20546..>21119)
                     /locus_tag="VE486_01535"
     CDS             complement(20546..>21119)
                     /locus_tag="VE486_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZA37800.1"
                     /translation="FPHDVHVSQLGMDCRYCHSFVDMAAHSNLPNTQTCMNCHTQVQK
                     DNPKLEPVRASWKTGNPIEWVQIHRTPDYVFYNHSAHVNRGISCFSCHGPVNHMPVVY
                     HAKPHSMAWCLECHRHPENFLRPDDQVFNLDWKPDDVKPAEFVAKYGQPQDAREDLSK
                     KKKLSQTEIGQTLKERWNINPPTNCQGCHR"
BASE COUNT         4929 a         5954 c         5909 g         4327 t
ORIGIN      
        1 tcgtcggtcg aatcatccag tatctggatc tggagaaggt gctgcggata gtccaattca
       61 ctgactgagc gaagcaaacg ctcggccacg taaacctcat taaagatcgg taactgcacg
      121 gtcaccttcg gcaattgttc aaatttgccg gcaggaacag gtgggcgcct cctgttcttc
      181 agatataagt agatgatgaa ataacggtga atgccgtaga ccgaaagtcc ggcgagcaca
      241 ctcagatagc agatggtcca gataatgtgt ccgaggcttc cttgcatggg agtttccgcg
      301 gccccgaaag ttttcggggc aagggcgtca tgatgccgtt tccaagcggt gcgtcaatgt
      361 aaaaatcgtg cgtttgtctt tgaaaatccc ggtcatataa tattcggtcg cgcgcaagag
      421 cgagagttaa acctcgcgaa tttcgtcagg ccgatcaagc ccaagcaccg ggatgcagcc
      481 gacattcgtc ttgcctgagg cggcgaaccg gtgaacggtg gtaagccaat tttcgcaacc
      541 gagagggcat gcaattgcga gacacattta agaaacgcag gcgggaccgt ttcgagcgtt
      601 tgaagatcat ccttccaatc cttctggcgg gatggatccc gtcgatctta accctcatcg
      661 tttcctacac gatcctgcgc gacactctcg aaacccgaat cctgcgcgac cggcaaaccc
      721 tcatccaact cgtgggccac ctcgttggcc atgatcttag ccggagcagc agtgtcattg
      781 agtattatca gacgctgccg gcagtcgccg aagttttaag cgcgccgatt cccgagccgt
      841 tcgcgcaaca atggctcaac gcagcatttt attcgcatcc gcaaatcgac ggaatgttca
      901 tcaccgcgtc agacggccgc ctgattggat cgatcccaac ggatccggag ataatcggaa
      961 aggattttga ttccaatcaa tggcgcgacc aggcagcggc gtccaatgag gcttttgttt
     1021 cggcggtcca tcccagattg caggacaagc ggctggccac cgatatcgtc ggcgccgtgc
     1081 gggcgccaga tcggacaatc ctcggttatg tgggcgtttc cgttctagta gaaagaatgg
     1141 gccggcgttt gtccacgatt gagtttaccg accgactcct tttcggggtg ctcgatcaaa
     1201 acggcaactc acttttcgcc agggatttca agccgaatcc caacgcgatt tcgccggaga
     1261 gtggcatttt gctcaaggag acacgccaaa gcacgagcgg gcatttcgag cgggagggca
     1321 atctttattc tttcagtccc atcgaatcca ccggctgggt cgcggtagtc gagcagccga
     1381 ggagtctggc ctacaaaccg gttcatgatc ttctcggacg aatgaccatc ctggccgtct
     1441 ggctgattct tctcacagcc gtcttcgcgt ggctcggcac cagattttat cagcggcaga
     1501 tggaatcgac gcgccgcatc gaacgtgaag ttgtctttaa cgaaaagatc ctggcgaaca
     1561 tgcccagcgg cattgccttt atcgatccga agtctcacca ttttctccag gcgaacgacg
     1621 cttttgcgca tatggcgcag cgtttcggag atttgccgcc cggtcgtgac atcacggaag
     1681 ccacctgcga cgacgtgaaa attgctccgg ctggagcgat cgagagagtg ctttcctttg
     1741 gcacaccatt tcagctcatc gagtacccgt tgaaagacaa aacgggtatg acgcattttt
     1801 tggatattaa cctcctgcgg ctgcagggtt cgcacgaaac cattcaaggc gtgctttatc
     1861 ttgtcgaaga caagacgcgc gacgtcaaac tgcgccagga gctcattgac gcaaatgcgg
     1921 caaaggacca attcctggcg ctgctttcgc acgaattacg caatccgctg tcaccagtga
     1981 tcgcgatggt tggagagctg gaagcgacca tcccggactc gccgacggtg cgggaggcgc
     2041 tcgaagtcat tcggcgcaac gtcgagctgg aagcgcgctt gattgacgat ctgctcgacg
     2101 tcacgcggat cgcaaaaggc aaactacagt tgagctttga gactgtttgc gtgaatcaaa
     2161 tcctgcaacg cgcttacgaa atttgccgcg aagacattgc ggccaaacat ctcgaagtgg
     2221 aatttcgtct tcgcgccacc gatacgcacg tcgaaggaga tcccgcgcgt ttacaacaag
     2281 tcttctggaa cttgatcaag aacagcgtga aattcactcc cgaaaaaggc cgcatcacaa
     2341 tcgaaaccat caatcccgcg cccgacaaaa ttgacattcg gattgccgat accgggatgg
     2401 gcatcgagca ggaaaggatc gacaaaatct tcaatgcctt cgagcaggga cagacttcga
     2461 tcacccgtcg atttggcgga ctgggcctcg gcctctcgat ttcgaaggcg ctgatcgacg
     2521 cgcatggcgg aaagatccgc gtagagagtt ccggaaagga tcaaggctcg actttttcgc
     2581 tcgaactgaa gacggtgcta agtccggtga gcggcgacgg cgatggggct aggagagatc
     2641 gacgcgctgc tttggaaaaa gacgcggacc tgccgagaca tcggcgagtc ctggtggtgg
     2701 acgatcacca ggatacctgc accggaatga agaagatgct ggagcgtcgc ggttaccaaa
     2761 ttacagtggc ccataccgct cagcaggccg tggaaaaggt gcgcacacag gagttcgatc
     2821 ttctcattag cgacatcgga ctgccggacc gcagcggcta tgagttgatg cgagaagtgc
     2881 aaagcactaa agcgcttccc ggaattgcgc tcagtggctt tggaacggaa caagacgtaa
     2941 atgaagccct ggctgcgggt ttctcagggc atctcacgaa accgatcaat tttgagcgac
     3001 tcgaggaagc catctccaat ttgcttgagc cggagccgac ttgccattca taagcgcacc
     3061 actggggcgc ggcactcctc tcgatcccgt ctttagcaaa acgttgtgcc aacaaagaac
     3121 gcgtcagcct atcaacctct ggattgggat ttcgggtttc tcgcagaaaa cgccgccgtt
     3181 cgctcaaaac ggttcaacat gacggccgag gcgaagagcg tccgttatcc catccggttg
     3241 ttgcgctact ggtttgccta tcatttgttg cgcatcgaat cagcccgtcg cgccgcaccg
     3301 atcgatctgg tcgagatcgg cgtacaaaag gcacaattcc ttcaattcgt tcgttcggcg
     3361 cccgttcagc caaatgttgg cagatggacg gtggtagatg cagtgatcca taccgaaaaa
     3421 tttcgcaaag tcgggatcaa gaaagaagat gttctgcagg cgaatctcga gagccccgat
     3481 ttcgatctgc cccacgcgta tgacgcggcg gtgttgttgc acgtgctcga gcatttattc
     3541 gaaccggagg cggctttgcg gaaaatcgtc cgccgaatac ggcaaggtgg cgtgttaatc
     3601 ggcggctttc cagtcctgcc tcattttctc gtcgctcctc accagaagcg ggagcgcaaa
     3661 actgcacagc caatgggcca tgtaagcgta ttttctccac agcgggtcat tcggatgggt
     3721 gaagcggcgg gttgcacggt tgagtttaat agcggcgcct tcttcatgcg gagcagcgga
     3781 agcccgctgg aaaattcagc tgcatggctt cgattcaact tatggtgggg cagcagattt
     3841 ccctcctggc caggagaaat ctattggctc atgcgcaaac gctgagcgtc gacgttccac
     3901 ccacttattc agccttccgt ctcctcgccg gcaaatcgga ttttcagcct catcataagc
     3961 tgcctacacc ccgacatctt tgctgtggat gcggtggcca atgtgtcagc cgcaatcgcc
     4021 ggattccgcg cggggggaca tgcgcctccg cgctttttaa tccaagtcat gcgagtaatg
     4081 ttataaaatc gaaatgactt ccgctcagga aaggaaaatc aagcagaccg gttgcgatgc
     4141 tcggtttgat aacctgacgc gacagcttta ctcgacggac gcgtcgattt atcagatcga
     4201 accggtcggc gcggcctttc cgaaaagcgc gcgggaagcg agcgcggtga ttcatgccgc
     4261 cgccgatacc ggcctgtccg ttattccgcg aggggctgga acgagtttgg ttggaaacgc
     4321 aattggcgaa ggattgattg tcgatttctc gcgctacaat cgccagatcg ccgaactcga
     4381 tgtcgaaaaa cgcagcgtcc gcgtcagtgc tggagtcgtg ctggatcagc tcaatgattt
     4441 tctcagacca cacgggtttt gttttggccc ggatgttgcc accagctcgc gcgcgacgct
     4501 cggcgggatg atcgcgaata attcctcagg cgcgcgttgc ccaatctacg gaacgaccgc
     4561 cgatcacgtg atctcacttg aaatcgtgat ggccgacggg cgcgtcgaga agatcggccc
     4621 aacgcacgaa tcgttgcgcg gcgagctcgt gaagatcgag aatcttattc gtgcgcgaag
     4681 cgctgaaatg gccgaacgct ggccaccggg cttgataaag cgctggcccg gctatggcat
     4741 cgaaaggttt ctccgtgctc cgaatgatct caccagcatt ctcgcaggca gcgaaggcac
     4801 gctcgccgcg atattttctg cggaactgaa aatcgcgcca ctaccgcggg agaaaggatt
     4861 gggcctgatc tttttcgcat cggtgggaga agcgatgcaa gccacggtcg aacttctcga
     4921 tctccaaccg gctgccatcg aacacatcga tcgcccgtta ctggatcaaa ccaaaggcca
     4981 gcttcatttt caggcggcgc gcgatttgct cgaactcgat tcgaagccct gcgaatcgat
     5041 tttgatcgtt gaattctatg acgacgtcac ggaccggttg tcgcttttgc aagcgagaaa
     5101 actcgggttg cgcacgaaaa tcctcaccga tccggtgcag atgaatttgg tctggtcggt
     5161 gcgcaaatcc ggtctctcgc ttttaacggg ctgcgtgggg ccagcaaagc cggtcgcgtt
     5221 cattgaagac gctgccgttc gcccggcgca gttgcccgat tacgtgcgcg gattgcaatc
     5281 aattatgaaa ccgctcggac tggaggcgag ctattatggt cacgccgcca gcggattgct
     5341 ccatgttcgt ccggttctcg atctgcacag cgcggccgat ttgaaaaaat tccggcaagt
     5401 cgccgatcaa acctccgcgc tggtgcggca attcaaaggt tcgctctccg ccgagcacgg
     5461 agtcggcatc gcgcgcaccg aatacatgcg cgaccaactc ggcgatgaac tgctcgaagt
     5521 gatgcgcgag atcaaaaatg cgttcgatcc gaaaaacatc ttcaatcccg gaaaaatttt
     5581 cgaggtagag tcggcgcgct gcgccgactc ccgcgctgcg ggacagcgca gcgtccctac
     5641 caagatcgac aatcatttac gcgaaaattt cacacgaccc ctagagcttc cattcgaacc
     5701 agtgctcgcc tttgcattca aagaccgctc gttcgtcggc aatctcgagc aatgcaacgg
     5761 ttgcggcggc tgcctgaaac acaccggcat catgtgcccg acgttcatgg caaccgggga
     5821 tgaagttatg tccacccgcg gccgcgccaa tatcatccgc gccgcgctcg agctgcgagt
     5881 gaatggacac gatccgctga aatgcgaaga gatcgatgct gccctgagca attgtctctc
     5941 ctgcaaagga tgcacgccgg agtgtccgtc gaacgtgaat ctcgccttgc tgaaagcgga
     6001 gctgatgtac gcgcgatatc ggcgcgacgg gttgccgttg cgagagcgaa tattcagtaa
     6061 cgtcgatttg ctcgggcgaa tcggctgcgc ggtgccgtcg cttgcgaatc tcattctcga
     6121 tttaaagccg ctgcgcgccc tgatggaaaa gacggttggt ctctcggcga aacgatcatt
     6181 gccacattat gcaaacgaaa gattcgatag gtggttcgaa aaacggattc gtcggctcga
     6241 cggagtctcg ccccaccggc aaagggggaa cgttatcctg tgggatgaca cctttgtgcg
     6301 ttaccacgaa ccgcacattg gaattgcggc ggtgaaagtg ctggaagcgc tcgggtttga
     6361 agtggccctc gtaaaaaacc ggcgctgttg cggacgacca gcatttagtc agggaaatct
     6421 cggtgccgct gcaaagctcg caaaacacaa cctcgatctt ctctcatcac tccaaaactc
     6481 caacactcca atccttttcc ttgagccgtc gtgctggtcg atgttcgtgg aagattatcg
     6541 cgagctaaag atccaaaagg ccgaggatgt ggcggcgcgg tgtttcctgt tcgaagaatt
     6601 cgtggacgat ttgctcgctc aggaaccgga cgcgcttcgt ttcagcgagc gtccggcgaa
     6661 ggttgccatc cacccgcact gccacgcgaa gtcaatcatg aatccggcat tcatgaaaac
     6721 actcgcggaa cgattaggga aacaagcaac ggtgctcgac acggcgtgct gcggaatggc
     6781 cggcgcattc ggcgcgctgg cagagaaata cgatctgtcg gtgcaagtcg ctcaacgctt
     6841 gctcgacaaa atcaatggcc aaccaccggg caccgaaatt atcgcttcag gcacaagttg
     6901 ccgccatcaa attgtcgatc tgacaaattt gcatccgaag catatggccg aattgctcgc
     6961 cgaagcgatc gcttaaagtt aattcggatt aatctggatt tcggaacgcg aaaactcgat
     7021 tcgaaaaagg caacttaagt tacccggctt ttccacgata ttgcatcgaa acttaaaggg
     7081 agaagatcgg tcagattctt gctgtgctcc cggtaagcgg cgaaacggga cgcagttcat
     7141 aaccgaatcg ccgctaaagg aaaggaagtt atggcaagcc cacttatccg ctgggaaccg
     7201 ctggaattgg aagatgtgca aaatcgattg tcgaagctct ttgggcgccg tccgaacggc
     7261 agagatgaaa tcacactcgc tgactgggcg ccgctcgccg acatcaccga agacgacagg
     7321 gaatatgtga tcaaaaccga gttaccggat gtaaaaaaag aagatgtaaa gattacggtc
     7381 gagaatggcg ttcttactat ctccggcgaa cgcaagttcg agaaggaaga gaagaaaaag
     7441 aaataccatc gagtggaacg cgcttacgga agctttatgc gcagcttcag tcttccggat
     7501 caagccgacg caagcaaagt taaagccgag ttcaagaatg gcatgctcac ggttcatctg
     7561 ccaaagagcg agaaggccaa accaaaacaa atcgaagtca acgtcgcctg acaaatcgtt
     7621 gcgggagacc gcggtgcatt ctgagacaat aggtcggtag gtcggcagat ccgtaccgtg
     7681 ctcgtctacg acacagtcga cgatcggttt gcgacataat aatccaatga gagcatcggc
     7741 atcgagggag ggaattgcag aagttccgtc gcaagcgatc cagttgttcg agccgccaga
     7801 ggacgccatt tacacgattg atgcaacagc gcatctcgtg ggcgtcccgc ggcggactat
     7861 tctcgtttac tgcaaacatc agcttctttc gccggtgatt aatcgcacgg acggaggtta
     7921 ttgtttcgat cgcgatggcg tccacgcgct gcgtcgcatc gaagcgctcc gagctgtctg
     7981 tggtgacgaa ctcagcggca tcaaaattat cctcgattta acgaaagagc tggaacgttt
     8041 gcgttcggtt gtgcgtttcc tgtcgcaaag caaatggcaa cgccagatga tgcccggtgg
     8101 aaaatatgaa acaccgccgc tcgggagaaa atcgagaccc catcgaggga ggaagtagtc
     8161 cactggatca aaattgctga ctaccgaccg cggccgccgg agcgatacgg gacgggaacg
     8221 tactccactg aaggctgccg tcgcatgggc tgtggataga gttccatcaa acgcagcatg
     8281 ttttccggca tctcggttcg tactggcaag ccgagttcgc gtgcccgctc gtaagtgatt
     8341 ggttcagcgg agccgcggct cccacgccca ttcgaggccg tactcatccg gttgcgattg
     8401 tctgcggcgg cggacaaaca gccagcacaa aagcattccg ctaaggaatc cacccacgtg
     8461 cgcccaccat gcgatcccgc cgacttgctg tggtccggct agggcaagcg ccccgctgaa
     8521 caattggctc aagaaccaga atcccagata cagcaccgcc ggcagctcaa agaagaaggg
     8581 gaaaaacaaa attggaaaca gcaccaccag ccgcgccgtc gggaaaaaga gcaggtacgc
     8641 gccaaggaca ccggcaatcg cgcccgaggc accaactgcc ggaaccgtcg aatccggatt
     8701 tgttagcaac tgcgtcagtc cggcgattac accgcagagc agatagaaaa tcagaaaccg
     8761 caacggcccc atccggtcct caacgttgtc gccgaagatt cagagcgtcc acatgttgaa
     8821 aatgatgtgc agccagccgc cgtgtaaaaa ttgataggtc aaaagcgtcc agtaactctc
     8881 gccgggaaaa ccgacgtagg cggcccaatc cggatgagta aaccgcgccg gaacgaagcc
     8941 gaaaatataa aacacttcgt cgatcgattg ctggccaagc atcaattcaa agaagaacac
     9001 gatcacgttg acaaaaatca gcgcgcgtgt gactaccggc accgaacggc tcggcaccga
     9061 atctcgaatt ggaaacatgt tgcctgcgtt aagttatcga ggtgaccgag cgcagcggtc
     9121 gctctgtgag cgataaaagc cggccgacgt atgcctattt caacaatcaa ccctgcgggg
     9181 ttcgactggc caccccgagt ttgcgaaaaa tagcgtgact cgcctggggg tgcgctagcc
     9241 ttgcggtttc ggcggctcgc ccggctttgc ggcttcgccg ggtttcggcg actcaccgcc
     9301 agcgggtgcg ccgccgggaa ggtcttcatc cggaactgcg agtatatcgt gttcgacgaa
     9361 tgactcgcca cggcctgcca attctttacg caaagcggca atttgctcag ccgtgaccgg
     9421 gctggcgtta ttgccccact cgctacgctc gtaggtgagg acatcggcaa ttttttggtc
     9481 cgtcaatgtt ttctcccaag gttgcatcac tgcagcgcca tattgctggc ctttcacctt
     9541 gacgggacct tgcagacctt taaggacaat catggcaggc cggcgcgagc cgccaatcgt
     9601 aaactcagag cccgccagcg gcggatattg accggcaacg ccaagaccat ttgcttgatg
     9661 gcaggtctga caattggccg cgaaaatctt cttgccgcga tcgcgcggcg atagttcggc
     9721 aatctgttct gcaccaggcg gtccggtaat ttttttcatc gtgcgcggct cgccgccgta
     9781 aggatccaac gcttcaccgg taaagtttcc cgagtaacgg ccgagatacg cgccaccaaa
     9841 aaataccgcc aagccgtaaa ctgcgattaa ccaaatcgag agcggttcgg cgccgacacg
     9901 cggctcgcgt ttttcccgct gaacagcggc gtgcacctgt tgcacgtcgg cttgttcgcc
     9961 gtagtccatt ccctggccaa ccccgaacgc tttcggaggt gtcggttttg gagtttcctc
    10021 gttcattgcg ttctcatttt gccggcgatg gcgatcccgc agctgccgga ctgggtgatc
    10081 cggctgctgc cgcgggcgcg gccgctctga cttctttcag gggatgtgat tgattgagcg
    10141 acattaaata ggcgacgaga cattttgcat caaacgtggg aactatctcc cagccttcgg
    10201 gtggcgcatc ggacccggtc aggtgtaaag cctcagcgga aggcgcatca ctgatgcggc
    10261 gcctttgata aagaaagcga taggagggca tgttggaatc gacgttgata ctgcgcggcg
    10321 agtaaagatg tacatgatgc caggccgcgg aatacattgg cggttcgcca gtcgtaactt
    10381 caggccacgg cgctgaagga cttggtgaag acggcggact ggagaccgcc gctccttgcg
    10441 ctggaggaga acccgccggt ggaggcgacg cggttgtctc cggagctggt gccgcgtttg
    10501 gcgctggcga tgtggcagct gccggaggag aacttgccgg cggaggcgac ccagctgcct
    10561 gcgcaggtgg tccacctggc gatggcgagg cgcctcctgg aggacttgga ctcgggccgg
    10621 caggagccgg actcgatgca ggcgctcctt cctgcggcac aggcgaggct gcgggacttg
    10681 ctgctccgct gggttgagcg ctttcctcct cagccggcgc gcgcgcgccg atgttcgcaa
    10741 gatcctggcc catccgcatt ttcccgagaa acactggccg ctcgaaaatg taatcgcgcg
    10801 gcgcactgcg gcggtcgccc cattttcgct cgatatcggc cgccgaataa tcggcgcgca
    10861 cttgctggct gtgacaatag atgcagccgt tcgcggcgta aacgcgcgcg cctcgctgga
    10921 ccattcccga ttgcggcatc ggataaatat ctgtgccttc ctcgtcggat tgcggattaa
    10981 gatacccgat ttgccaattc ggaatgacag tcagtcccac ccatgagaag gcgaacgtgc
    11041 cgaaaatccc caggaaaaga ggagcgagtc ctttcatggc gcttaccttc ggcggactgg
    11101 agaccgccac tccttggaac gagcaaaagc aacgagttcc ttcataagtg cgcttcggaa
    11161 ccttccgcct ccaccgggtt gagaaatgtg ggaacagtcg atttccgacc caaccccaaa
    11221 agcattagtc caaagtgata cgcgaaaata aaatgtgacg cagtgagcaa aataccggac
    11281 agactacgtc cgcgcagata aggaaggacc gtttcagtgc tctcgctgaa aacgagcgcg
    11341 gggttttcca tgttcgcccc ctggagaaac ccgccggcga gcagcatgag cgtcataaga
    11401 ccgatgccat aagctgaagc ccagaaatgc agcttaatca gtgaagcgta acgccattct
    11461 ctcccaatca gtcgcggcac gatgtagtac atcgacccga aaatcaccat cgtgaaaaac
    11521 gcatataagc caagatgcga ataagcgatg ctcgcctgag agaagttcac gtagcgcgcc
    11581 accgagcgca gcgagatcaa aacgccgaca acgctaaaaa ccgtgtacgc catcgcgcca
    11641 aataccgtga accgcagcgt ggggctgtag cgcatgagcg gaaaatagcc ctgcatggtc
    11701 atgtgatgat tcaggccgac ggtggcgacc ggaatgatcg tgaggatagt cgccgcgatg
    11761 ctggcggtaa tcatccacgc cgggaatggg ccatcgacca gccgctgcat tccggtccag
    11821 ctggagaaaa gcgcgtagct ccaaaaaccg atcgccgcca gatgataact gtaaaccggg
    11881 cgaccgatca ctttgggaat catgtaatag gcggtaccaa gcccgatcgc gccgaaccag
    11941 agaaagagca gattattcgc gtaccaccag cccaccgccg attgcaagac tccctgcacc
    12001 ggtacgacga agagcatcaa ctggccagcc gcgtagagcc acggaaacca aagaaacgcg
    12061 ccgagcaaat accattgcgt aatgtaaatt tgttctccac gccgataccg aaacatcagc
    12121 actgcccatg agacgacgag cgtgtaagcg acaaaaagaa tgatcgctgc gtagtgcggg
    12181 aactcgagcc attgatagcc ggtgctgtcg ccgagaacga ttccgcccac acccagcagg
    12241 actcccaaat tccaaaatcc agcgcccgca accaggagta gcggaaagcg cagaaccgtc
    12301 cggcacaatc gggccatcag ccaaatcgcc gtgcccattc cggccatcga cgcccagcca
    12361 taaaccatca cgttcatgtg ggctggccgc actcgtcccc acgtgaggaa actaattccg
    12421 gaaagcagat cgggcgagtg cagcttgaac gaaacaaaac cggccagcag cgtaccaagg
    12481 attagccacg caatcgccga cgtgtagaac atcaacaccg gcacgcgcgt tgacgcatcg
    12541 atcaaggcgc gctccagttg ctcggcgtcg cccgcggccg cggaaacgga cggttctttt
    12601 aaaggaattc ccgtggcgtt catggagtct tggctggctc gggcgacgga gatcctggtg
    12661 gtaattgttg agcttgcggt gaaggcgaca ctggagcttg ttgcgagggc gatgcagccg
    12721 gttgcgtggc gctcggttgc ggagaaccgg ccggcgcagg actcgcctga gcggctgggg
    12781 cctgttgttc cggtgtagcg atcggaccgc ctggcgctgg tttttgctgc gccagctcgg
    12841 caacagtcaa ttccatcgcc cgctctatcg ggatgcgagc gacgcctttg ttcttgtcga
    12901 tccagccata agtagtcaac gctttggcgg cctcctcgcg cgcggtcttc aagttttcca
    12961 tgcgtttttt ggcccgcgtt tcctcgtaat cgcttccgcg cggcccagga ccgatcacgg
    13021 cgagcacgat cacaccgaaa agcatgaaaa gaagcacaat ccccaaccac gtggacaacg
    13081 gggcccgcga gtggacaacc tggcgaagag attcggtgtc ggccatatca gttggttaat
    13141 tccaaagatt caatcaagcg cggatcgcgg actggaaaga gtgaggtcct gggcacgagt
    13201 cgcagataaa cgaaaccgag cgttgcgccg atggcgatga gcgaaaccag atcccagatg
    13261 ctcacctgca caccgccgcc atgtaacgcg ggcaatacga tgatgtacat gtcgagcatc
    13321 tgcatgcaga caatccatcc agctacgatg caaagccggc gcggctcctt tttgatcgag
    13381 cgcaggagca gaaccccaaa aggtatgaag aaacgcccaa tgacgaggag catgctcagc
    13441 gcccaccacg attcggtgtt tcgggtaatg aaaaattgcg tctcctccgg gatgttcgca
    13501 taccagatga gcatgtactg gccgaaaccg atgtaggccc agaaaataca gaaggccagc
    13561 atccatttcc ccatgatgtg ataatgctcg cgcgtgacca catccttcag ataaccagcc
    13621 tggcgcagag ccgtgatcac cagcaccagc aaggacatcg agctgccggc cgcgcccgcg
    13681 aagatgtaaa cgccaaacat cgttgaaaac caggtatagt tcaggctcat cagccaatcg
    13741 aacgcgccga aggtcagaca aagcgcaaac atcggaaggc tgataaacgc ggccttgcgc
    13801 atcccaattg tgaagcgcgg attaccgtcc ttgtcctgtc gcacggagaa ccagcgcaaa
    13861 gcgagcgctg ctgcggcaaa aaagccgaga aacaaaacgg cgcgtccgag aaagaaaccc
    13921 cagttaagat aagcgcgctt ggaatctaat aacgcctcgt gtccgggcgg aatgttcatc
    13981 catgcgtaaa gatgatgccg aaggagaaga attggcacga aaagaagcgc caggactaca
    14041 agcaatgccc cgaggttttc caactggcga cgcaccacca ccgaccattc agcatcggtg
    14101 gcatgatgaa cgatcgtcca gaaaaagcag cccgcgcaca gcgtgaagaa aaatgcaaag
    14161 gcaaagagcc atgaataact gaattgatgc ggattcagga atgcgccgac gatgcagagc
    14221 gccagcgcca ccacggcgat taatccgaga acgaacgaaa ggcccgcgaa gcggccgctt
    14281 tcgaaatatt cgccctccgg cgttggtacg gtttgggaac gttcgctcat tgtttttcaa
    14341 gttcagcgcg atgatcctcg ggaacatccg caactcccgc ccgttggctg cgctgcaagg
    14401 cgcgcaggta agcgatgatc gcccagcgat cggcgacaat gatgttcgac ccgtaagcca
    14461 tcatggtgtt cttgccgtgg gtgatcgtgt tgaaaatttc gccgtcggac atcttgcgga
    14521 ttcggtcgtc ctgaagagtc accacggttg caagaccgta ttgtttcgtg attccgttac
    14581 cctcagccgc ggcgccatga cacatcgcac aagtgatgct aaaacgctgc cggccgcgct
    14641 ccatcaactc ggaggtgact tccagcggaa taccggtccc ccagtgatcg cccatcttgc
    14701 ctgtgttgta ataatcggtg ccggcactaa agctggcatg tggatgcgac cagggaccga
    14761 tcattggccc cgatgcctca attgtccccg gtttgggcat ctcgtagcca atcggaactg
    14821 tgccagcaac cggcaaacgc ggaccgcgtc catcggcaaa caaattgagc ggcgcttgcg
    14881 cgcgcacttt catctgacgg accatgtcgg gaaaaagctc gagcggcggg ccggtgctgg
    14941 tttgtccgcg aaacccaaac acggcaatga tcgcgatcgt gcacagaaga aaaatcacca
    15001 gaaatccgcg caacatattt caatcctcgt ggacgatcgt gatgtgctgc ccgccgcttt
    15061 gttccagtaa ctcgcgcacg ccattttcgg taaagcgcgg atcgcgcgct tcaatcgcca
    15121 ggaaaaatcc gtcattggtc acgcggctga accgttccca gttaaacatg ggatgatacc
    15181 agcgcggcaa tccgttcatg ccgaacatcg cgaaaaacgc ggcgaatgct ccgaaaagca
    15241 cggtcaattc gaacatgatc gggaaaaacg ccggaacggt gaaaaaattc cacggtttgc
    15301 catggacatc gagcgggtag agaaaccacg acgggccgaa ttggacgacg gcagcggtca
    15361 gcaaacctga gactccgcca aacaaaaccc atccgctcaa ccaggatttt cccagcccca
    15421 tcgcttcatc cattccgtgg atggggaagg gagaatagac gtcccaacgt ttgaagcccg
    15481 cgtcccggac acgcttggcc gcttcgtaaa gcgcagccgc gctcggatat tccgcagcca
    15541 tcgcatacac ttcatgaccg cctggagtct tcatcgtgtg cgctcctttc cgccagcggc
    15601 tgccggatgc tgctcagtgg gagagacata atgcgggtcc gctgccggca cagcggtctt
    15661 cacttccgac atcgcgatca taggcagaaa acgaacgaag agcagaaaga gcgaagtgaa
    15721 aattcccagc gtgccgatgt acgtccagat gtccacccag gtcgcatgaa acattcccca
    15781 tgacgatggc aggaagtcgc gatgcaatcc gccgacaata atgatgaagc gctcgaacca
    15841 catgccggcg ttcacacaca tgcagacgaa gtaaacgacg aaaatatttc gccgacacca
    15901 tcggaaccag aagagttgcg gtgagagaca attgcagaaa agcattccca cccaccagta
    15961 ccaccagtag ggaccgaaga cgcgattcca agacgcgaac cgttcgtaag agttaccgct
    16021 gtaccatgaa acgaaaaatt ccatcatgta ggcgtaggca acgaaggaac cggtgagcaa
    16081 aataatcttt gccatcttgt cgatgtgctg tggggtgatg acgtcctgta gcttgaacat
    16141 ggcgcgcgcg ggcagcagca aggtcaaaac catggcaaaa ccaccgaaaa tcgcgccggc
    16201 cacgaagtag ggcgggaaaa ttgtggtgtg ccacgttggc aggatcgacg tggcgaagtc
    16261 gaacgatacc acgctgtgca ccgaaagaac gagcggtgtg gaaaggccgg ccaacaggag
    16321 atacgccatc tcgtaatgac gccagttgcg attcgagccg cgccagccgc acgcgaaaat
    16381 tccgaaaaga aactttctaa ttttcgtcgt ggcgcgatcc cgcaacgtgg ccaaatcggg
    16441 gatcaaaccg gtgtaccaaa acaaaaccga gaccgtaaaa taggtcgaaa ccgcaaacac
    16501 gtcccatacg agggcgctcc ggaagtttgg ccagatcccc cagttgttag gcaccggcgc
    16561 caggtaccat gccatccaga cacgccccac gtgcacgcca gggaaaattg cagcgcaaat
    16621 aaccgcaaaa agcgtcatcg cttccgcaga ccggttaatg gaagtacgcc atttttggcg
    16681 gagcaagaac aagatggcgg agatgagcgt gccagcgtgc ccgataccga tccagaaaac
    16741 gaagttcgtg atgtcccagg cccaaccaac cgggatgtta tttccccagg tgccaacgcc
    16801 ggtagaaatc tggaacccca ggcaaaaaag accgaatgtc gcgacacttg cggtgatggt
    16861 aaaagctacc caccaccagc gtggcgcggg ttgctccacg acgccggaga ttcgttccgt
    16921 gagccaactg aagttgcgcc tgttcaagac cagcggaaca tgcgcgagcg gtcgctcggt
    16981 ctctttaatc atttccggtg ctgtcggggc tgcgtccatt tcaaattgcc aattgccaat
    17041 tgccaattgc cgattgccaa aagcgagccg ccgcgccgaa tcgaaaattc atcatcctgg
    17101 tttctcctcc aggcttgcaa cgccgatgtt tgccgcgtcc ggcattttcg gattcgggtt
    17161 gcgaatgcgc gcaagataac tcgttcgcgt attcacgttc agatactcga gcagccgata
    17221 attgcggtcc tgtcctttca ttttcgaaac gcgactttcc ggatccttta tgttgccaaa
    17281 aacaatcgct tccgttggac aggcttgcgc gcacgccgtg gtaaaggaat cgcgcgggat
    17341 aagtgtatcg ggtgaagcgc cggctcgcgc ttgggccgcg atttttgctt cctcaatccg
    17401 ttgcacgcaa aacgtgcatt tctccattac cccgcgcatc cgcacggtga cgttcggatt
    17461 tttttgcatc ttgataatgt cgggcgcacc tttggtggtg agcggcgcga aatattcttc
    17521 atagatgctc agggcaccgg cgatcttcct cttgccgact ggtcgctgat tgtaatcgaa
    17581 gtaattgaat cggcgcactt tgaatgggca gttgttcgag caatatcgcg tcccaatgca
    17641 gcggttgtaa gccattacgt tgagaccatc ctcgctgtgg agggtagcgt taacgggaca
    17701 aaccgtttcg catggcgcgt tctcacagtg ctggcacatc atcggttcgt gcacgatctc
    17761 cgggtcttcc ggccaatcgc cgcgatcctg gttgaatggt tttctgctcg cataatagcg
    17821 gtcgatgcga atccagtgca tcacccggcc atgagccacc tgtaacttgc cgactatcgg
    17881 aatgttgttc tcgctttggc aggcgatcat gcacgcgctg catcccgtgc agacattcag
    17941 atccactgtc atcccccatt gctgcggtgc gtcgagcggc gggtgactgt agatggttgg
    18001 caacctgtgg tgcagttctt cctggccagg ccctttcttc gcgaaatcgt tgtcggcccg
    18061 gtagcgttca agcgtcgctt cccgaataag accgcggcct tcaatgctga attgttcttg
    18121 cgtgatagag agcggataag tgcgtcctac cttcgtcact ttcacgctct caatcgcctg
    18181 gccatcagcg gtggcgaaat atggatttgc cgctgtgcgc aggaagtagc cgttgaaacc
    18241 cgactgttca gcgatccccg gtctctcttt taaccctccg ccggcatacg gtagtgcact
    18301 gaactctggc atttttcgcg cgtaaccgag cgggatcgtg atcgaattat ccgcatgacc
    18361 aggcgacacc agcgcggcga taaccagctc gcgtttgatc cgccctccgc gagcgctctt
    18421 ttcattaatc gcgatcttca ccaggtcgcc ggtttggacg ctgagccttt tcgcgaaacc
    18481 ggggctcatc agcgcggcgt tgtcccaggt cagtttcgtg attgggtcgg gcaattcctg
    18541 gagccagccg ttattggcgt agcggccatc gcccatggca taactgccca cgaggacgat
    18601 ctccggcgat tctgctgaag gcgccggtgg cgacaacgcg gaactttgag cgagaaggcc
    18661 tgccgcgttt ccattaaacg agggcggctg atcctggatt tgaacgtggg acgcaaatcc
    18721 atcgtggaga aggcgggacc aggcggtgtc gaaatctccc ggtggattcg tcgcacgaaa
    18781 agtatcctgc accagctcgg gtccctctgt tttcggtcca ccgaggagtg cgttcagcag
    18841 ctcgatttcc gataagccgc caaaaagcgg cagaatcatt ggttggatcg acaggtattc
    18901 gccctcggct gtgagcgcgt caccccagga ttccagataa tgcgcagcag gaatatgcca
    18961 ttggctcagc gccgaagtgg aatcctcgtg ataaccgagc cgcacaatct ccggcacctt
    19021 tttttgcaga tcactccaat caagcggctg ccctgtctca ttgtcctcta ccagcccacg
    19081 cggcgcgtta tagaccgggt ccccttcgaa aacgaacaat tgcttgatcc gcccgtcatt
    19141 tatgtccgcc gccagttggg aaatgtcgat cgttcgggga ttccgcggga cctggcggac
    19201 gaccaatgtc tggccaagat ttttgagcgc agcgttaatc gcataagcga gaatatgcac
    19261 ctcgctcggc tgacctgggc ctgccagcac cagactcgcg cctggcttcg ccatgagatc
    19321 attcgcagcc tcgctgagcc attgctcatc gaatttggcg gcgcctggcg gcagctgcac
    19381 attcgcaacc acagaaccga gactcgtgtc gttcgttcgc gccaggatct tcccggccag
    19441 cgcgcgcgcg aacgccgcaa tctggctggc cgggcagcgc agacgatggt cggccatcgc
    19501 gccggtgagg gtgaagcggt tctcgactac ataaagccgg ttcatgttat ctttcggcgc
    19561 gctcactcgg cgtcgtgaag tgaatgcgcg gaccgacgcg agatcgccct ggccgcaatc
    19621 gagaaaatcg ctgtcgagcg cgagaatcac atcggcctga tcgaatttcg ggaccacgcg
    19681 cacgttgtca ccgaagagac cctggacggg ggagcttcgt tcctggctca gaagcggctc
    19741 gtaaacacac cagcgcacgc tcgggaaaat cttttccagc tccgcgcgca aacgttcgcg
    19801 agtgggggaa tgcgtttcgg taaccagaaa cgcaaggccg tcgccctgcc gcgtgctcaa
    19861 tgcgttgcga agcttgccaa tatattcgtc aaacgccgcg cgatcgctct ttttcccttt
    19921 gtgaacgaac cgttgcgagc gcgatggatc atagagatca agaatcgatg cctggacaaa
    19981 ggtgtcggtc gcgccgccgc ttgccggatg caatggattg ccttcgagct tgatcgggcg
    20041 tccgtccaca gtcgttgcga tgagcggaat cgcgccggtg cgccgggcca tggcggtcgc
    20101 gtaatacaga aattttccgg gaattgtcca ctcgacattc ttcgtgaatg gaaccagatg
    20161 ggcttccggt cgccggcaac catttaaacc aaaaccagcc agcgccatcg atgcgcccat
    20221 caatttcaaa aaactccgtc gcgaccattc gtcacccttg agttcggctg cgcccgcggg
    20281 aaattcgtgt tccagccact cgcgaaattc gggtgtatcg ctcaactcgc cgagactgcg
    20341 ccagtaacgt tttcccgttg atggctccgg tggatgatgg aatacgcgtt tcatgggagg
    20401 ataggcatct tgcctgtctc ggcaggcaag cttccagctt gcttcggaat aattgccgat
    20461 gttcgccgcg ctggaagcgt ggcggccgag tcaggcttga agcctaactt ccgataccgg
    20521 cgcattaact tagtcattgg cgttttcatc ggtggcaccc ctgacaatta gtcggcggat
    20581 taatgttcca gcgttccttt aacgtttggc cgatctctgt ttgagagagc tttttcttct
    20641 tcgataaatc ttctctcgca tcctgcggct gtccgtattt cgccacaaat tctgccggct
    20701 tcacgtcgtc cggtttccaa tcgagattaa aaacctggtc atctggccgc aaaaaatttt
    20761 ccggatgccg atgacattcc aggcaccacg ccatgctatg cggttttgca tgataaacca
    20821 ccggcatgtg gttgaccgga ccgtgacagc tgaaacaact gatgccgcga ttgacgtgcg
    20881 cgctgtgatt ataaaaaacg tagtcgggcg tgcgatggat ctgcacccac tcgattgggt
    20941 tgcctgtttt ccagctcgcg cgcaccggct cgagcttcgg attatctttc tgcacctggg
    21001 tatggcagtt catgcacgtc tgggtattgg gcagattcga gtgcgccgcc atatcgacaa
    21061 agctgtgaca gtaacggcaa tccattccca gttgcgatac gtgcacgtcg tgcggaaaa
//
LOCUS       DASQUQ010000056         8642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1004942, whole genome shotgun sequence.
ACCESSION   DASQUQ010000056 DASQUQ010000000
VERSION     DASQUQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004942"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VE486_01540"
     CDS             <1..1140
                     /locus_tag="VE486_01540"
                     /inference="COORDINATES: protein motif:HMM:NF044853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HZA37801.1"
                     /translation="VEPSLGTLADFDWLQKQIRKRGMEIALDFAINCSPDHPYVKEHP
                     DWFYKRPDGTIKYAENPPKKYEDIYPLNFRCENWRELWAEMKSIVLFWAQRGVRIFRV
                     DNPHTKPVAFWDYLIQGVREKYPDTVFLAEAFTKPKMMKALAKAGFSQSYTYFTWRNS
                     KRELIEYFTELTQTEMSEYFRPNLWPNTPDILPFVLQDGGRPAFMIRVALAATLSTLY
                     GIYSGYELCENEALPGREEYLDSEKYQFKERDWNAPGNIKDWIARLNKIRKENRALQL
                     YDNLRFYPAENEAILFYGKMTAARDNIILVVVNLDPYRKQHSYVDVPVDQFGQMETDA
                     YEVHDLLNEARYIWRGRRNYVELDPNIQPAHIFRVRRWASGDRFV"
     gene            1228..1998
                     /locus_tag="VE486_01545"
     CDS             1228..1998
                     /locus_tag="VE486_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZA37802.1"
                     /translation="MKSSLLLFATLAGAPLLLAQEATPPLVPAVPHSQTIMDTLINAG
                     PVMIPLFLLSIFFVALVIVYLMTIRRGAVVSSGYMATADALLRKRDYLGLLAVSNRHG
                     EAIARVVQRMLDFTTKNPNADFQQVREIAETEGTRVAASLNNRVIYLADIGMIAPLLG
                     LLGTVFGIIHSFGALGADIGSARYVALSRGISQALVNTAAGLAIGIPAMMFYAYFRGK
                     AQKLISELEAASTHVLALLSLHYSRRAQRTPVLIEDEL"
     gene            2050..2457
                     /locus_tag="VE486_01550"
     CDS             2050..2457
                     /locus_tag="VE486_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZA37803.1"
                     /translation="MNLRGHAPMHHPGIQLAPLVDVLLLLLIFFLLTWNAARTENELD
                     VRVPKASAAKEKSAPIGDVIVNVKADGNVVVNRRTLSGPELTELLKSLVQLNPEQAVV
                     IRGDEAGAYKNIIGVLNVCTEAGITNVAFATAK"
     gene            2454..4916
                     /locus_tag="VE486_01555"
     CDS             2454..4916
                     /locus_tag="VE486_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA37804.1"
                     /translation="MNTVRTAIGPLGLAILFSMAGVALAQSPPEVRKARPVDEPPVAR
                     ALPVEQAPPIMPPAPAEDFSGPPATESEAPDRRQLDYANALFTRKLYDLAIPEYQKYL
                     DDYPGNSGRANAYFSLGECHRNLNRPSSARTNFKKVLSDYGNSEFAGPAAYALAEMAF
                     KEKDYAAALPLFHRSAAKSKESAVALSARYFEARCLENLGRKEEASDIYLDVAEAGNP
                     NPYREDARWTAASILIGRGRKIDALKQYEALSNETQKPAVKAESAVRGGVIALELVQA
                     DKGKIDKAMVERATTLLQRGRNLSEGKFRAIAQVGLRRLQYQTGQYAQLLADYKKEQE
                     NLPEAAQAEVLLLAANSQRQLGHAKEAEQLYRQIIAKFPDREEAKDAAYQRLINVYNS
                     DPSALLIEAEQFLATNPTGERADQAKLLKAEALYKQQKYSEAASIYGELRSSQLSSKL
                     RAESAYKLGLCYVPAKNTSAVIEAFTYYVQTFPDNLQVPAALAQRALAYEQDKNYDAA
                     LSDLNTILSKYPDAQEREATLQLKALILGQQENTKGMVDTFRQLLKEFPKSSVAAQAQ
                     YYIGKAAFEAKDYKTAMKALNAARQLNKDQYYNLASLRLILCQFYLKDRPALTKEVND
                     FMANSPNVNVPPEVLEWLGIEYYNDKNFEAATKYLSALGKIDNRGSVKPDFLFYLGDA
                     ATKLKNLPDAEDAFGKYLQVAKDPAGKAKVLLALGAVKIGVHKPDDAQKIAEEIMVLQ
                     PEGRVNAEARLLAGEVQLERGNFDDAGKAFVGVALLYDDPAITPRALDKAASAYQRAG
                     RSEEADRLGRELRERYPNYAGG"
     gene            5017..5679
                     /locus_tag="VE486_01560"
     CDS             5017..5679
                     /locus_tag="VE486_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HZA37805.1"
                     /translation="MTKFRPLLAEFIGTFALIFVGIGAIKTAGHDVLAVALAHGLTIA
                     AFVSATLHISGGQLNPAVTFGLVCGRHMKWTNALPYWIAQCLGGISAALICRGLFGRD
                     VVITGTPQLAINLAPWQGILVEAILTFFLVFVVCGVAVDERGPRRIAGLVIGATITLD
                     ILFGGTLTGAAMNPARVFGPAVAANFWHDHYVYWIGPLVGAALGGFAYRVLIERRPAT
                     GT"
     gene            complement(5773..6300)
                     /locus_tag="VE486_01565"
     CDS             complement(5773..6300)
                     /locus_tag="VE486_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37806.1"
                     /translation="MFAAIYLPNFYLQAAIRHQPELRAQPVALLEEEENKPVIIQLNE
                     AAEQAGIRKGMSPSQALARYLQVTIKARARPQEKSIQEMLLHYAFTLSPFVEATKPGI
                     CTIQFTDNRDLSANVSRVIEQLAQCEITAQAGIAPTPDTSLLIAHLARPVLQVENPKE
                     FLAPLPIETLAMIGT"
     gene            complement(6300..6974)
                     /locus_tag="VE486_01570"
     CDS             complement(6300..6974)
                     /locus_tag="VE486_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37807.1"
                     /translation="MPFFLMATCGKIIDLRNLLANRFPSTPPQAGTRLITGLSFLDQT
                     IGGGLPKGAITELISPRRSAGSASLIHALLHAAQRGRYFLALIDGRDSFDPQLPGGNA
                     CLRHLLWVRCSKALDAIKAADLLLRDGNFPLVIVDLVLNPPPDLSEIPQTTWYRLQRL
                     VERAPAACLVMTRQGMVSSAQLKIVLENSWTLETFEDESAISRLQIRVQRSQLHRQAT
                     QIGGAS"
     gene            complement(7033..7647)
                     /gene="lexA"
                     /locus_tag="VE486_01575"
     CDS             complement(7033..7647)
                     /gene="lexA"
                     /locus_tag="VE486_01575"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZA37808.1"
                     /translation="MLTERQKAVLDFIQREQREKGITPSTREIQDHFGFASQTSVMQY
                     IGALERKGFLDRHARKARALITPAMKVRIADIPIYGQIPAGMSSLTEQIIEGHVSLDR
                     RSANVSKNRGTFALRVRGDSMIGAHIVDGDIVILEESKEAHNGDIVAALLDGETTLKR
                     YVVEHGRPYLKAENPRYPDLIPARELKIQGVMVSLIRKQERRKR"
     gene            complement(7901..8437)
                     /locus_tag="VE486_01580"
     CDS             complement(7901..8437)
                     /locus_tag="VE486_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZA37809.1"
                     /translation="MIYATRIHGGKLRKKTRIPYIGHLLGVTAIALEYGANETEAIAA
                     LLHDAVEDCGGVKRLRDIERKFGRKVARIVKGCTDTDQTPKPPWRKRKENYIAHLKQA
                     SASTRLVSAADKLHNARAILHHLRQEQDSLWPRFNGGKEGALWYYRSLVSAFREHGSN
                     ALIEELDRVVTEIEELAK"
BASE COUNT         1904 a         2375 c         2379 g         1984 t
ORIGIN      
        1 gtcgagcctt cactcgggac actggccgat ttcgactggc ttcaaaaaca gattcgaaag
       61 cgcggaatgg agatcgcgct cgacttcgcg atcaattgct cacccgatca tccttacgtc
      121 aaagaacatc ccgactggtt ctacaagcgc ccggacggca cgattaagta cgcggagaat
      181 ccgcctaaaa aatacgagga catttatccg ctgaactttc gatgcgaaaa ctggcgcgag
      241 ctatgggcgg agatgaagag tatcgttctt ttctgggccc agcgcggtgt ccgcatcttt
      301 cgcgtggata atccgcatac caaacccgtc gccttttggg attatttgat ccagggcgtc
      361 cgcgaaaaat accccgatac cgtctttctc gcggaagcat tcaccaagcc gaaaatgatg
      421 aaagcgctgg ctaaagctgg cttcagtcag agttacacct atttcacatg gcgaaattcc
      481 aagcgcgagc tcatcgaata tttcacggag ctgacgcaaa ccgagatgag tgagtatttt
      541 cgacctaatc tttggccgaa cacgcctgat atcctgccgt ttgtgcttca ggacggcggg
      601 cggcccgcgt ttatgattcg tgtggctctg gccgcaacgc tttcaaccct ctatggcatt
      661 tatagcggat acgagttgtg cgagaacgag gctttgccag gtcgcgaaga gtacctcgac
      721 tccgaaaaat atcaattcaa ggagcgcgat tggaatgcgc cggggaacat caaggactgg
      781 atcgcgcggc taaacaaaat ccgaaaggag aaccgcgccc ttcagcttta cgataacctg
      841 cgattctacc ccgccgagaa cgaagcaatc ctgttttatg gcaaaatgac tgccgcgcgc
      901 gacaacatca ttctcgttgt tgtgaatctc gatccatatc gcaaacagca ttcatatgtc
      961 gatgtgccgg ttgatcagtt cggccaaatg gagaccgacg catatgaagt acacgatctg
     1021 ctgaacgaag cccgctacat ttggcggggc cgccgaaact acgtggagct cgatccgaac
     1081 attcaaccgg cgcatatttt ccgggtgcga cgctgggcca gtggagatcg attcgtttag
     1141 caacctggcg aactgttctg tgtttgatat gtcagtcgtg catttgcatc ccttcatcgc
     1201 tccgcatatc attacgaaat tcctggcatg aagtcttcgt tgctgttgtt tgccacgctg
     1261 gcaggcgcgc ctctcttgct tgcacaagag gctacgccac ccttggtgcc ggctgtcccg
     1321 cactcgcaga ccatcatgga tacgctcatc aacgccggcc cggtgatgat tccgctgttt
     1381 cttctgtcga tctttttcgt ggcgcttgta atcgtttatc tgatgaccat ccggcgcggc
     1441 gctgtggttt cgagcggtta catggctaca gcggatgcgc tcttgcgcaa acgcgactat
     1501 ctgggattgc tcgccgtttc caatcggcac ggagaagcca tcgcgcgggt cgtgcaaaga
     1561 atgctcgatt tcacgaccaa gaacccgaac gcagattttc agcaggtccg tgaaatcgcc
     1621 gaaacagaag gaacgcgagt cgcggccagt ctgaataatc gtgttattta tctcgccgat
     1681 attggcatga tcgcgccgct cctcggtctg cttggcacgg tctttgggat tatccattcc
     1741 ttcggcgccc ttggagccga tattgggtcg gctcgttacg tggccctctc gcgagggatc
     1801 agtcaggcac tggtcaacac ggccgcaggc ctggccatcg ggattccggc gatgatgttt
     1861 tacgcttatt tccgcggcaa agcccaaaag ttaatttccg aattggaagc cgcttcgacg
     1921 catgttttgg ctttgctttc gttgcactat agccggcggg cccagcgaac ccctgtgctg
     1981 atcgaggatg aattgtaagc cgcccagctc gccaacaatc cgccagagcc atatcgtctc
     2041 cttcgttata tgaatttgcg tggacacgca cccatgcatc accccggcat tcaactggcg
     2101 ccgctggtcg atgtgctgtt gctgttgttg attttttttc tgctcacatg gaacgcggct
     2161 cgcaccgaga atgaactcga cgtcagagtg ccaaaagctt ctgcggcgaa ggaaaagagc
     2221 gcgcccatcg gggatgttat tgtcaatgtg aaagcggacg gcaacgtggt tgtgaatcgt
     2281 cgcactctga gcggtcctga attgaccgaa ttgctgaaaa gtctggttca actcaatccc
     2341 gagcaggctg tagtgatccg cggagatgag gccggcgctt acaagaacat cattggagtc
     2401 ctgaatgttt gcaccgaagc tgggatcacc aatgtggctt tcgcgacagc gaaatgaata
     2461 cagtgagaac cgcaatcggt cctttgggac tggcaatact gttttcgatg gcgggagtgg
     2521 ctcttgctca atcgccgccc gaagtgcgca aggcgcggcc agtggatgag ccgccggtcg
     2581 cgcgcgcgct tcccgtggaa caagctcctc caataatgcc tcctgccccg gcggaagatt
     2641 tttccggacc gccagcaaca gaaagcgaag cgcccgatcg ccgccagctc gattacgcaa
     2701 acgcgctttt cactcgcaaa ctttatgatc tggcgatccc ggaatatcaa aaatatctcg
     2761 acgattatcc cgggaattcc gggcgggcaa atgcttattt ttcgctgggc gaatgccatc
     2821 gaaatctcaa tcgcccatcg agcgctcgca caaacttcaa gaaagtgctg agcgattatg
     2881 gcaatagcga atttgccggc cccgccgcat atgctctcgc ggagatggcc ttcaaggaga
     2941 aggattatgc cgctgccctt ccgctctttc atcgttccgc cgccaaatca aaggaatcag
     3001 ccgtggcgct gtccgcgcgt tattttgaag cgcgttgcct ggagaatctc ggccgcaaag
     3061 aggaagcttc tgatatttat ctggacgtcg ccgaggcggg aaacccgaat ccctatcgcg
     3121 aggatgcgcg atggaccgca gcatccatcc tcattgggcg gggaagaaaa atcgatgcgc
     3181 tcaagcaata cgaagcgctt tccaacgaaa cacagaagcc ggctgtaaag gccgagtcgg
     3241 ccgtgcgcgg cggcgtgatc gcactcgaac ttgtgcaggc tgacaagggc aagatcgaca
     3301 aggcaatggt cgaacgggcc acaacgttat tgcagagggg ccgtaatctg tctgaaggaa
     3361 aattccgggc gatcgcgcaa gtaggtctgc gccgcttgca atatcaaacc ggccagtacg
     3421 cgcagttgct cgctgattac aagaaggagc aggagaacct tccggaggcg gcgcaagctg
     3481 aagtgttgtt gctcgcagcc aacagccagc ggcaacttgg tcacgcaaag gaagcggaac
     3541 aactttatcg ccagattatt gcgaaatttc cggatcggga ggaagccaag gacgctgcct
     3601 atcagcggct gatcaacgtc tacaactccg atccatctgc actcctcatc gaggcggagc
     3661 agttccttgc cactaatcct acgggcgagc gcgccgacca ggcgaagctc ttaaaagccg
     3721 aagccctata caaacaacag aaatacagtg aggcggcctc gatttatggg gagctgcgtt
     3781 cctctcagct ttcttcaaag ttacgcgccg agtcggctta caagcttggc ctctgttacg
     3841 tgccggcgaa aaacacttcc gcggttatcg aagctttcac ctattatgtg cagacatttc
     3901 cggacaacct gcaggtgccc gccgctttgg cccagcgcgc tttggcttac gaacaggaca
     3961 agaactacga tgccgcgctt tccgacctga acactattct tagcaagtat ccggacgcgc
     4021 aggaacggga agcgacgctg cagttaaagg ctttgattct cggacaacag gaaaacacaa
     4081 aaggcatggt ggatacattt cgccagctct tgaaagaatt ccccaagagt tccgtcgcag
     4141 cgcaggcgca atactatatc ggcaaggctg cgtttgaggc gaaggactat aaaacggcga
     4201 tgaaagccct caatgccgcg cggcaactga ataaggacca gtattacaat ctcgcctcgc
     4261 tccggctgat tttgtgccag ttctatttaa aagatcggcc tgcgctcacc aaagaggtaa
     4321 acgatttcat ggccaacagt ccgaacgtga atgtgccgcc agaagttctc gagtggcttg
     4381 gcatcgaata ttacaatgac aaaaacttcg aagccgccac aaaatatctg agcgcgcttg
     4441 gaaagatcga caatcgtggc agcgttaagc ccgacttttt gttttatctg ggagacgcgg
     4501 cgacgaagct gaaaaacctg cccgatgcgg aggacgcttt cggcaagtat ttgcaggtcg
     4561 ccaaagaccc agcgggaaag gcgaaagtgt tgctcgcgct cggcgcggtc aagatcggcg
     4621 tccacaaacc cgacgatgcc cagaaaattg ccgaggagat catggtgttg cagccggagg
     4681 ggcgcgtaaa tgctgaggcg cgcctgctgg cgggtgaggt ccaactggag cgcggaaatt
     4741 tcgatgacgc gggcaaggct ttcgtggggg ttgctctgct ctatgatgat ccagcaatta
     4801 cccctcgcgc gttggacaaa gcggcatcgg cctaccaacg cgccgggcga agcgaggaag
     4861 cagatcggct gggccgggag ttgcgcgaga gatatcctaa ttacgccggc ggttaacggc
     4921 gcggccgtca gacgggctca gcggcgcgct tcgcaatagt gaagcggcca tagaaacaga
     4981 attgccagcg cgccgctgat ttgattgcat tcacgcatga ccaaatttcg tccgctcctc
     5041 gcagaattta tcggaacatt cgccctgatt tttgtcggca tcggcgcgat caagactgcc
     5101 gggcacgatg tgctcgccgt cgcgcttgct catggcctta cgattgcggc ttttgtctct
     5161 gccacgctgc acatctcggg aggccagtta aatccggcgg tcacctttgg tttggtttgc
     5221 ggccgtcata tgaaatggac gaacgcgctt ccctattgga tcgcgcaatg tctcggggga
     5281 atctcggccg ctttgatttg tcgcggtctt ttcggacgcg atgtcgtgat tactggcact
     5341 ccacagctcg ccattaatct cgccccgtgg cagggcatac tggtcgaagc gattctcacg
     5401 ttctttctcg tgtttgtggt ttgtggagtg gctgtcgatg agcgggggcc acggcgcatc
     5461 gctggtctcg tcattggagc aaccatcacg ctcgatatcc tgttcggggg gacccttacc
     5521 ggcgcagcca tgaatcccgc gcgcgtgttc ggtccggctg tagccgcaaa tttttggcac
     5581 gaccattatg tttattggat cggaccgttg gtgggcgcgg cgctgggcgg ctttgcttat
     5641 cgggtcttga tcgaacgacg acctgcgact ggaacataac gctgcgcctt gtgcgacaag
     5701 cggacatgct gttcgcagtt gtttggattt aacctcgaaa gctttcgggg gcttgccgca
     5761 cagactaaaa gtctatgttc cgatcattgc gagcgtctcg attggcagcg gcgcgagaaa
     5821 ttcttttgga ttctcaactt gcaaaaccgg acgcgccaga tgcgcaataa gcagacttgt
     5881 atccggggta ggggcaatcc cggcttgggc agtgatttca cattgggcta attgctcaat
     5941 tacgcgcgag acgttcgcgc tcagatcgcg attgtccgta aattgaatgg tgcaaatgcc
     6001 gggcttggtg gcttctacaa acgggctgag ggtaaaggcg taatgaagta acatttcctg
     6061 gatcgatttt tcctgggggc gcgcgcgtgc cttgatcgtg acctgcaaat agcgcgccag
     6121 ggcctgactt gggctcattc ctttacgaat gccggcctgt tccgcagcct cgttcagttg
     6181 aatgattacc ggtttgtttt cctcttcctc aagcaacgcc accggttgtg cgcgcagttc
     6241 cggttgatgg cggattgctg cctgcaaata gaaattcggc aaataaatgg ctgcaaacat
     6301 taactcgctc ctccgatttg agtagcctgc cggtggagct gcgatcgttg cacacgaatt
     6361 tgcagccgcg aaatcgcgct ctcgtcttca aaggtttcca gcgtccacga attttcgaga
     6421 acaattttga gttgcgcact gctgaccatc ccttgccgtg tcatgaccag acatgcggca
     6481 ggggcccgct cgacgaggcg ttgcaatcga taccaggttg tctgtgggat ctcgctcaag
     6541 tctggcggcg gattgagcac cagatcgaca attaccaatg ggaaatttcc gtcgcgcaaa
     6601 agcagatcag ccgctttgat cgcatcgaga gctttactgc aacgcaccca cagcagatga
     6661 cgcaaacacg cgttgccgcc gggtagctgc ggatcgaatg aatcccgccc atcaatcaga
     6721 gcgaggaaat accgaccgcg ctgcgcagcg tgcaacagcg catgaatgag cgatgcgctg
     6781 cccgcgcttc ttcgtgggct gatcaattcg gtgatcgcgc cttttggcaa tccgccgcca
     6841 atcgtttgat cgaggaaaga taaaccggtg attagacgcg ttcccgcttg gggaggcgta
     6901 cttgggaaac gattcgcaag aaggttgcgc agatcaataa tcttgccgca cgtagccatc
     6961 aagaaaaatg gcatgggcgg gagacatgtt caagcgaaca ttaaagttga actaacgcac
     7021 ccacccgtct cattacctct tccgccgttc ttgtttgcgg atgagcgaga ccatcacgcc
     7081 ctggattttt aactcgcgcg ccgggatcag gtctggataa cgcgggtttt ctgctttcag
     7141 atacggccgg ccatgctcta ctacgtaacg cttcagcgtg gtttcaccgt cgagcaatgc
     7201 tgccacaata tcgccgttgt gagcttcttt gctctcctcc aaaattacga tgtcgccatc
     7261 gacaatatga gcgccgatca tcgagtcacc acggacgcgc aaagcgaatg tgccgcgatt
     7321 tttggaaacg ttagccgacc gcctatcgag agacacgtgg ccttcgatga tctgctctgt
     7381 gagcgacgac atgcccgccg ggatctgccc ataaattgga atatcggcga tcctgacttt
     7441 catcgctgga gtaatcaacg cccgggcttt gcgcgcgtgc cggtcaagaa aacctttgcg
     7501 ctccagcgcg ccgatgtatt gcatcaccga agtctgactg gcgaacccga aatgatcctg
     7561 gatttcacgc gtgctgggcg taatcccttt ttctcgttgc tcgcgctgga taaagtcgag
     7621 gacagctttt tgtcgctccg tcagcatacg ttcaggatcg cgcaccattc gggcaatgca
     7681 agttcaagtg aacagcatct gtttccttca acctactata gcttcgcggc gatctcctcg
     7741 gaaagccggc actgacgacc agtttcttcg gatctggaac tttacgcagg tcggggcgcc
     7801 gatcgcgggc gcggtcgccg tgacgataag gaatactctg ccgaggcagg ctcgccacgg
     7861 cgccggacgc aggcatgtcg ccggcgctcg acgcatcttt tcatttggct aattcctcga
     7921 tttctgtcac gacgcggtcg agttcttcaa tcaacgcgtt gctgccgtgt tcgcggaacg
     7981 ccgatacaag tgagcgataa taccagagcg ctccttcctt gccgccgttg aaccgcggcc
     8041 aaaggctgtc ttgctcctga cgcagatgat gcaggattgc gcgggcgttg tggagcttgt
     8101 cggctgccga tacgagccgc gtcgatgctg aagcttgctt cagatgcgcg atgtaatttt
     8161 cttttcgttt tcgccatggc ggcttcggcg tttgatcggt gtcggtgcag cctttgacta
     8221 ttctcgccac ttttcttccg aactttcgct cgatatcgcg cagccgtttc accccgccgc
     8281 aatcttccac cgcatcatgc agcagcgccg cgatcgcttc cgtctcgttg gcaccgtatt
     8341 ccagcgcgat tgcggtcacg ccgaggagat gcccgatata gggaatccga gttttcttcc
     8401 gaagtttgcc gccgtgaatt cgcgtggcgt atatcaacgc tttttcaaac tgaggcgata
     8461 gtcgcacgtt tcactttagc cactcgcgtt caaaagaaaa tcagggcgct ttgaagttag
     8521 tcggatacta aagatcaatt cccatcgtga ccgcgccaga aatctcttcg aagccagagc
     8581 gcttccaaaa ttcacggccg atcggattat cctcagccac catgataagg acgcgcttga
     8641 cc
//
LOCUS       DASQUQ010000057         5913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_918086, whole genome shotgun sequence.
ACCESSION   DASQUQ010000057 DASQUQ010000000
VERSION     DASQUQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918086"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /gene="lepA"
                     /locus_tag="VE486_01585"
     CDS             complement(<1..1143)
                     /gene="lepA"
                     /locus_tag="VE486_01585"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZA37810.1"
                     /translation="MAIELTRNFCIIAHIDHGKTTLSDRLLERTGTIHEREKQDQLLD
                     SMDLERERGITIKAHPVMMHYTSKSGERYRLNLLDTPGHVDFAYEVSRSLAACEGALL
                     VVDAAQGVEAQTVANVHLAHKQGLTIIPVINKIDLPNADLASVHRQLEEILAIPSEEA
                     IEASAKMGIGIEEILEAIVKRIPAPQKPLDATLRALVFDSVFDVYRGVVGYVRVVSGT
                     MEANHAIMLMSNNARYEIKEVGVFTPKMLVQPKLNPGDVGYFIANIKTTADIKIGDTI
                     TDQRHPARGPLPGFQEIHPMVFSGIYPINTGDFEHLKTAIGKLSLNDAAFVYQPESSV
                     ALGFGFRCGFLGLLHMEIIQERLRREYNMDIIATSPSVVYEVVTTRG"
     gene            1318..1809
                     /locus_tag="VE486_01590"
     CDS             1318..1809
                     /locus_tag="VE486_01590"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZA37811.1"
                     /translation="MPRLIISGTAHELAEDLITVGRSPDNMIVINDPSVSTHHAQLQR
                     AGETYRLKDLDSTNGTRVNDLPAAETSLRFGDRIRFGAAEARYEPDMSGSQPLPQPEK
                     IIARSAELSAVPADFINASPFPRQREENDSFRTAISIALAVLALIFLGSMIAVLTMHA
                     PSL"
     gene            1870..2562
                     /locus_tag="VE486_01595"
     CDS             1870..2562
                     /locus_tag="VE486_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZA37812.1"
                     /translation="MTKIKKRLLLFDIDGTLIHSGGAGVHALKSAFTERFGIVDDLHD
                     IEIAGMTDSGIVVSILNKHKIPATPENVSAFLDSYVHFLSLELPRRVGKLLPGVLELL
                     EKLKSRPQLVLALLTGNVSRGAQLKLEHYGVWHFFEFGAFADDHHDRNRLGTVARARA
                     KEKHGRQFSTSEIDVIGDTPRDIACGKAFGARTIAVATGTWKRNQLAKYEPDFLIDDL
                     SNVQRIIDTLGW"
     gene            2585..2935
                     /locus_tag="VE486_01600"
     CDS             2585..2935
                     /locus_tag="VE486_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZA37813.1"
                     /translation="MKKPVPSPVPMPRKDHPNVLPLEGEIDLHVSPAVTAAINTVIEK
                     KPKQLVIDLSRVTYIDSAGLATFIEGMQKIEAYGGKFALAGLQETVRSIFEISRLDQV
                     FQIFPDVDTALAAA"
     gene            complement(2893..4020)
                     /locus_tag="VE486_01605"
     CDS             complement(2893..4020)
                     /locus_tag="VE486_01605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZA37814.1"
                     /translation="MSNAKLSIAFVRRGYSQSGGAEAYLKRLANGVVEGGHDAQLITT
                     KAWPDSEWPFGVIRRLPSRSVIEFANQLEQMRPQIGCDVLMSLERIWSCDVYRAGDGV
                     HRAWLARRRKFELPLERFVRGLNSKHRHILQLEEALLANRSAARVIVNSQMVKTEIVD
                     LYGYPVDKMDIVRNSIPVGQFRFDPNLREESRDALKVKPDEIALLFAGSGWERKGLRF
                     AIAAMALCHNRKMRLLVAGRGNQRYYKSKRVNFLGEVPDPVRLYSAADIFILPTIYDP
                     FSNACLEALACGLPVITTRSNGFSEIIEDGLHGSVVDLASNVTGLRDAIQFWSQAARR
                     AAVRSTNMQHAAQFDISVNVARTLEILVQAAASAVSTSGKI"
     gene            4244..5404
                     /locus_tag="VE486_01610"
     CDS             4244..5404
                     /locus_tag="VE486_01610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZA37815.1"
                     /translation="MPRHLLYSIFARIGGSGLDTDAFETLRASYRGGFLGKAIAYDNR
                     QREIPASLIHSLRWHPVRLISFLDRPYYYAAKKRYLDWIASRHLATGRYDLFHSWSGD
                     CLRSLRVAKQRRIPSIVEIPTWHRDGGKAKIGRPQIAESTSNAWAFQRWKADLLLERK
                     RFTEEYELADLLLVLSQTAADTFRMRGFAEEKLFYLPRGVDVKRFKPGPRPAKFRAVF
                     AGALIKRKGIQHLLEAWHHLNLPDAELWLVGSVHDEAKPYLKQFWRDNIRIIGFVRDP
                     ETYLGQSTVHVFPSQWEGSAKVTYEAAACALPQITTREAGDVVRDDVEGIIVKPRDVD
                     AIAAALDHLYRHPDIVERMGNAARRRVVENFTWDHFRTRLLDAYEIAMRMAR"
     gene            5488..>5913
                     /locus_tag="VE486_01615"
     CDS             5488..>5913
                     /locus_tag="VE486_01615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZA37816.1"
                     /translation="MKILLLQLKRIGDLILTIPAIATLRENFPTAQMTLAISKECADL
                     LPAIPNVDRILLVRRNLRDLALFLALAREKFDYCVDFTRTDRSALLAFLSGARKRIVY
                     RRRDRFQKRARVYSDYVKVRKLAMHTIDYNLALVGPLGVR"
BASE COUNT         1279 a         1692 c         1608 g         1334 t
ORIGIN      
        1 accacgcgtg gtcacaactt cataaacgac gcttgggctg gtggcgatga tatccatgtt
       61 gtactcgcgc cggagccgtt cctggatgat ctccatgtga agcaaaccaa gaaatccgca
      121 gcgaaatccg aatccgagcg cgacggaact ttccggttga tagacgaacg cggcgtcgtt
      181 caggctcagc ttcccgatgg cagtcttcaa atgttcgaag tcgccggtat taatcggata
      241 gatcccgcta aacaccatcg ggtgaatttc ttgaaacccc ggcagcggcc cgcgtgcggg
      301 atgacgctgg tcggtgatgg tatcgccgat ctttatgtcg gcagtcgttt tgatattggc
      361 gatgaaataa ccgacgtccc cgggattcag ctttggctgg acgagcattt tcggagtaaa
      421 cacgcccact tccttgatct cgtaacgagc gttgttactc atcagcatga tcgcgtgatt
      481 cgcctccatt gttccggaaa ccacgcgcac gtagccgact accccgcgat agacatcaaa
      541 gacggaatca aaaacgagcg cccgcaaggt ggcatcgagt ggtttttgcg gcgccgggat
      601 tcgtttcaca atcgcctcga gaatttcctc aataccaatc cccatctttg cgctcgcctc
      661 gatagcttcc tcggacggaa tcgcgaggat ttcttccagt tgccggtgca ccgaagccag
      721 atcggcgttt ggcagatcga tcttgttgat aaccgggata atcgtgagcc cctgtttgtg
      781 cgccaggtga acattggcca ccgtttgcgc ttccacgccc tgggccgcgt ccaccactaa
      841 tagcgcccct tcacatgctg ctaggctgcg cgaaacttcg taggcgaaat cgacatgccc
      901 gggcgtatcg agcaagttca gccgatacct ttcgcctgac ttgctcgtgt aatgcatcat
      961 cactggatgc gcttttatcg tgattccccg ctcgcgctcc aagtccatgg aatcgagcag
     1021 ctggtcctgc ttctccctct cgtgaattgt gcccgtgcgc tcaagcaatc gatccgacaa
     1081 agtcgttttg ccgtgatcga tgtgcgcgat gatgcaaaaa ttgcgtgtta attcgatcgc
     1141 catcccgaaa actttcggga ctatgcgggc gcggccatgc gtggtcaatc gcggggagcg
     1201 cacgcatctt gcgtgcagcg ttcggcatgt tgccgaacga atagacttcg cagcaagatg
     1261 ccgcgaagtg cacgcaagat gcgtgcgctt ccagcaagaa tcgattatgt tgataatatg
     1321 ccgaggctaa tcatcagcgg caccgcgcac gaactcgccg aagatctaat cacggtcggg
     1381 cgtagcccgg acaatatgat cgtaataaac gatccatcgg tctcgactca tcacgcccag
     1441 ttgcagcgcg ccggcgaaac ttaccggtta aaagatctgg attcgaccaa tggcacgcgc
     1501 gtcaatgatc tcccggccgc ggaaacgtcg ctgcgcttcg gcgatcggat tcgatttggc
     1561 gcggccgaag cccgttacga accggacatg agtgggtcgc aaccgctgcc tcagcctgag
     1621 aagattatcg cgaggtcggc ggaactgagc gcagtcccgg ccgatttcat aaacgcgtcc
     1681 ccattcccgc gtcagagaga agagaatgac tcatttcgca ccgcaatttc tatcgcgctc
     1741 gccgttttgg cgctgatatt tcttgggagc atgatcgccg tgcttacgat gcacgctccc
     1801 agcttgtagc ggcggtctat gaccgtccgt ccaatgaatg cgacgttcac agacttcgtt
     1861 ccaggagaca tgacaaaaat caaaaagcgc ctgctccttt tcgacattga tggaaccctt
     1921 atccattcgg gcggcgccgg tgtgcatgcg ctcaaatcgg cgttcacgga gcgcttcggc
     1981 attgtcgatg atctgcacga catcgaaatc gcgggcatga ccgattctgg aatcgttgtc
     2041 agcatcctga acaaacataa aattccagca acgcccgaaa acgtcagtgc ctttctggat
     2101 agctatgtgc attttctctc gctcgaattg ccgcgccggg tgggcaagct gcttccgggc
     2161 gtgctggagt tgctcgaaaa actgaagtcg cggccgcagc tggtgctggc gcttctcacc
     2221 gggaatgtct cacgtggggc gcaactgaag ctcgaacact atggcgtctg gcactttttc
     2281 gaattcggcg cctttgccga cgaccatcac gaccgcaacc ggcttggaac cgttgctcgc
     2341 gcccgggcaa aggaaaagca tggccgccaa ttctccacat ccgagatcga cgtaatcggc
     2401 gacacgccgc gcgacatcgc ttgcggcaaa gccttcggcg cgcgtactat tgccgtcgca
     2461 accggcacat ggaaacgtaa ccagcttgca aaatatgagc cagatttttt gatcgatgat
     2521 ctttcgaatg tccagcgtat aatcgatacg ctcggatggt aagcccgcaa cgattgatgc
     2581 ttgaatgaaa aagcccgttc cttccccggt tccgatgccg cgtaaggacc atccaaatgt
     2641 tctgccgctc gaaggcgaaa tcgatctgca tgtctcgcct gccgtcaccg cggcaataaa
     2701 tacagtgatc gagaaaaagc cgaagcagct ggtaatcgat ctgtcccgtg tgacctacat
     2761 cgacagcgca ggactggcca cgtttatcga gggaatgcag aagatcgaag cctacggcgg
     2821 caagtttgcc ctggccggcc tgcaggaaac ggtgcgctcg attttcgaaa tctcgcggct
     2881 tgatcaggtg tttcagattt ttccggacgt ggacaccgcg ctcgccgctg cctgaaccag
     2941 aatctccagg gtccgcgcga cgttcactga aatatcgaac tgcgccgcgt gttgcatgtt
     3001 tgttgatctc accgcggcac gccgggccgc ctgcgaccaa aattgaatcg catcgcgcaa
     3061 tcctgttacg ttgctggcca agtcgacgac ggagccgtga agtccgtcct caataatttc
     3121 actgaagcca ttgctacgcg tcgtgatcac cggcagcccg caagcgagtg cttccaggca
     3181 ggcgtttgaa aacggatcgt aaatcgtagg cagaatgaaa atgtccgccg ccgagtaaag
     3241 ccggaccgga tccggaacct cgccaagaaa attgacgcgt tttgatttgt aatagcgctg
     3301 gtttccccga ccggcgacga gcagacgcat tttgcggttg tgacagagcg ccatcgctgc
     3361 gatcgcaaat cgcaaaccct tgcgttccca acccgagccg gcgaagagca gggcgatttc
     3421 atccggcttc acctttaacg catcccggga ttcttcccgc aggttcggat cgaaacgaaa
     3481 ttgcccgacg ggaatactgt tgcgaacgat gtccatcttg tccaccggat agccgtaaag
     3541 atcaacgatt tcggttttga ccatttgcga attgacgatc acgcgagcgg cgctgcgatt
     3601 tgccaggaga gcttcctcga gctgcaagat gtggcggtgc ttgctgttga gacctcgaac
     3661 gaatcgttcc aacggcagtt caaattttct tctgcgtgcc aaccaggcgc gatggactcc
     3721 gtcgccggca cggtaaacgt cacaactcca gatgcgctca aggctcatca agacatcgca
     3781 gccgatctgc ggccgcattt gctctaactg gttggcaaac tcgattaccg atctggacgg
     3841 caagcggcgg attacaccga agggccattc actgtccggc caagccttcg tcgtaataag
     3901 ctgcgcatcg tgtccgcctt cgactactcc gttcgcgagc cgctttaagt aggcttcggc
     3961 tccaccgctc tgcgaatagc cgcgccggac aaaagcgatg cttagcttgg cgttcgacat
     4021 tgtgatcctt taccaatctt gtcattccga gcgaagtcga ggaatctctc actgctaacc
     4081 tctttggtgc taacgataag aaatgtctcc gctcgatagg gcgatagggc acaagacgcc
     4141 actttacatg ctccccgttc gccagaatag ttctaacccg atgccgggtg aaactttaaa
     4201 cgctccgcct ccagttggtt ctgccttgcc agcggctcgt aatttgccga gacatttgct
     4261 gtactcgatt ttcgcgcgca tcggcggttc cggtctggac accgatgctt ttgaaacgtt
     4321 gcgcgcgtcg tatcggggcg gatttctcgg aaaagcaatc gcttacgata accggcagcg
     4381 ggaaattccc gcgtcgctga tccattctct gcgctggcat ccggtgcgac tcatctcgtt
     4441 cctggatcga ccttactatt atgccgcgaa gaaaagatat cttgattgga tcgcatcccg
     4501 gcatctggcg actggacgct acgatctgtt tcacagctgg tcgggcgatt gtttgcgctc
     4561 gctgcgagta gcaaagcaac gccgcatccc atcgattgtc gagattccaa cctggcaccg
     4621 cgatggcgga aaggcgaaaa ttggccgccc gcaaatcgct gaatcgacca gcaacgcctg
     4681 ggcgtttcaa agatggaaag ccgacttgct gctcgagcgg aagcgtttta ccgaagagta
     4741 cgagctcgcc gatttgctgc tagttttatc acagacggcc gccgacacct ttcggatgcg
     4801 cgggttcgcg gaggaaaagc tattttacct gccgcgcggg gtggatgtga aacgcttcaa
     4861 gccggggccg cgtccggcga aatttcgcgc ggtttttgcc ggagcattaa ttaagcgcaa
     4921 gggcattcaa catttgctcg aagcatggca tcacctcaat cttccagacg ccgagttatg
     4981 gctggtcggt tcggtgcatg acgaagcgaa gccgtatctg aaacagttct ggcgcgataa
     5041 tattcgcatc atcggttttg tgcgcgatcc ggaaacttat ctcggccaaa gcacggtcca
     5101 cgtctttccg tcgcagtggg agggtagtgc caaagtcacc tacgaggcgg ccgcctgcgc
     5161 actgccgcaa atcaccaccc gggaggcagg cgatgtggtg cgcgatgatg tggagggaat
     5221 catcgtaaaa ccgcgagacg ttgacgcgat tgcggcggca cttgaccatc tctaccggca
     5281 tcccgacatc gtcgagcgaa tgggtaacgc agccaggcgc cgcgtagttg aaaatttcac
     5341 ctgggaccat tttcgaacgc gattgctgga cgcttacgag atcgccatgc gaatggcgcg
     5401 ataatttttg gtagcggccc cgaatgcttt cggggtcgca ttaatccttc cggcggtcag
     5461 aggccgcccc tacagttatt tcggcccgtg aaaattctgc tgcttcaact caaacggatc
     5521 ggggacctca ttttgaccat cccggccatc gcgactttgc gcgaaaattt tcccactgcg
     5581 cagatgacgc tggccatttc caaagaatgc gccgacttgc taccggccat cccaaacgtc
     5641 gatcgaattt tactggtacg gcgcaatttg cgcgaccttg ccctctttct tgcgctcgcc
     5701 cgggagaaat ttgattactg cgttgatttc acgaggaccg atcgctcagc gttgctcgcg
     5761 tttttgtccg gagcccggaa acgcattgtc tatcgccggc gggatcggtt ccagaaacgg
     5821 gctcgggttt acagcgacta cgtaaaggtc cgcaagctgg ccatgcacac gatagattat
     5881 aatctcgctt tggtcgggcc gctcggcgtt cgc
//
LOCUS       DASQUQ010000058         7558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1005692, whole genome shotgun sequence.
ACCESSION   DASQUQ010000058 DASQUQ010000000
VERSION     DASQUQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005692"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VE486_01620"
     CDS             complement(<1..380)
                     /locus_tag="VE486_01620"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HZA37817.1"
                     /translation="MKSKRTERSFPFLRINERERKPRKHGLTEIRGPYYSVVGRRYLE
                     DLFETMGSYVDSLKFAGGSFTLMPEKAVEEIVDLCHKYDVLVSTGGFIERVLAQGSEA
                     VRQYVAECKKLGFDIVEISAGFIS"
     gene            complement(480..740)
                     /locus_tag="VE486_01625"
     CDS             complement(480..740)
                     /locus_tag="VE486_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37818.1"
                     /translation="MKKPKIDDKLTLLADFGKTEAICVEVLDNPATEEGILLKVMARG
                     PFEQGQQVWIVDRDGSKIGATVENVFKQTIDSEVTLSTVLPA"
     gene            831..2051
                     /locus_tag="VE486_01630"
     CDS             831..2051
                     /locus_tag="VE486_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA37819.1"
                     /translation="MARYRITQIEDYEQFIGREQVQRIRENAQRFKGTRVANFNSTYY
                     GGGVAETLSSLTLLMNSLGLRTEWRVIEGTPDFFSITKKMHNALQGGEIDLSSIKKEI
                     FEQVIYENSVRNFLEHDFVIVHDPQPVPLIEHYEKKCPWIWRCHLDLSRPNAETWKYL
                     LGWIDNYDAVIESCPEYNQEMKPPQRVFMPAIDPFTIKNRQLSDQEIDERLAHYEIPT
                     DLPLVAQISRFDPWKDPEGVVKAFKLARKQVDARLVLLGNFATDDPEGARIFETLRAC
                     QDESILILTSGDDTALVNALQTRAAVVLQKSLREGFGLTVAEAMWKGTPVIGGNVGGI
                     RYQIENGVNGFLVSSIDEAAERIVRLLKDEKLREEMGRKARKSVRDKFLLTRYLEEYL
                     DLFGEFDKDFRLRE"
     gene            complement(2396..2947)
                     /locus_tag="VE486_01635"
     CDS             complement(2396..2947)
                     /locus_tag="VE486_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HZA37820.1"
                     /translation="MNSFPSWLVALILLSLTTVTARAQTPGEKSSAPLKAVAMLHPTG
                     GNKVSGKVIFTEEADGVQIKAEITGLTPGNHGFHVHEFGDCSAADATSAGAHFNPTKQ
                     PHAGPDASARHVGDMGNIEADASGNAKLDYVDHHISLTNDQRSVIGRSVIVHEKADDL
                     KSQPTGDAGGRVACGVIGIAKGQ"
     gene            complement(3025..3780)
                     /locus_tag="VE486_01640"
     CDS             complement(3025..3780)
                     /locus_tag="VE486_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZA37821.1"
                     /translation="MLASRTDSSCPVKLTFLGTRGEIEARTRRHRMHTSLLVSHRGAD
                     VMIDCGLDWLGKFERLNPCAIVLTHAHPDHAWGLKDGAPCPVHAPQKTWQTLQGCSVE
                     DRRVIKERMPAKICGITFEALPVEHSILAPAVGYRVSAGRSRIFYAPDLIFIRDRAAA
                     LKGVQIYIGDGATLTRSFIRKRGKHLIGHAPVRTQLTWCAREGVPRAIITHCGSEIVS
                     GDERKILAKVHAMAAERGVEASIACDGMKLILR"
     gene            3913..5325
                     /locus_tag="VE486_01645"
     CDS             3913..5325
                     /locus_tag="VE486_01645"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HZA37822.1"
                     /translation="MLTTLLICAGAFSAVAQEPARTSQGAIASFAPIIEKVAPTVVTV
                     FTTQSVSQTVLPFPFSDDALREFFGGQLPQRQGKQTLEGLGSGVIVSADGYILTANHV
                     VAKADEIMVGLGAELRRYKANKVGTDPGTDLALLKIDEKNLPAITFADSEKARAGDVV
                     LALGNPFGLRQTVTMGIISAVGRGGMGIVDYENFIQTDAAINMGNSGGALVDTEGRLL
                     GINTAIFTRTGGSQGVGFAIPSNLARDVMQSLREKGRVVRGYIGTSVQTLTPELADAF
                     KLKELSGALVGEVSPKSPAEKAGIKSGDVITSVNGKKIGDARELRLMIGSMAPGTKVK
                     IEVNREGQKKTFGVDLAEMPAAAAEPAPEASPEQGAEPEKSTVFGGIVVADVTADVRE
                     ALTLPKEIQGAVIVAIDTASPAAQAGLRMGDVIQEVNKQPVKNAKDLVALSNKLKPTE
                     KILMRVYSQGRSGYVALEPK"
     gene            complement(5354..5785)
                     /locus_tag="VE486_01650"
     CDS             complement(5354..5785)
                     /locus_tag="VE486_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HZA37823.1"
                     /translation="MPYRYLEEIGTADIAFEATGRGLPELFVAAADATMNVMIENLDA
                     IEPRETRRIELKNDAIDMLLFDFLQELIFFKDAEQLLLRVREAKIDENDGQYSLKTTV
                     AGEKLDPGRHEQRADVKAVTLHQFRVEKTDDQWKASVILDI"
     gene            complement(5879..6811)
                     /locus_tag="VE486_01655"
     CDS             complement(5879..6811)
                     /locus_tag="VE486_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZA37824.1"
                     /translation="MLRTPITLVTGPLGSGKTTLLRHILATRPAKIAIVMNEFGEIAI
                     DTKVVEGKNVRIAELGGGCVCCSLLGEFEAAVNEIIAKVAPEMIIVETTGLAEPEALV
                     FNIQEALPQCRLDGVVSVIDADMLVRFPELGHTTRLQIEGADILLLNKIDLVEPKQID
                     PLEAKLREINSTAAIVRTERCRIDPGLLFGVGREKKVAPPEHKHQSEFESFTFESDKT
                     FDRDCFERFADALPKSVHRAKGFVRFPDGAQLFNFVVGRWELEPFESAETALIFIGKN
                     VLSEKEAILRALNECTVKNDEARMTKDEGMSKHE"
     gene            complement(6899..>7558)
                     /locus_tag="VE486_01660"
     CDS             complement(6899..>7558)
                     /locus_tag="VE486_01660"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZA37825.1"
                     /translation="IKVLDRELACAPFDSPEGRDYFAAMKCGLNMSFANRQVILHRIR
                     EVFSEILGRSAEDLEMHMVYDVAHNTAKLEKHTVDGKEKTLLVHRKGATRAFGPGHHE
                     VPVRYRDIGQPVIIGGSMETGSYLLVGAPGGDQTFFSTAHGSGRTMSRTKARKQWRGQ
                     KLLHEMEARGIYVRTTSMRGLAEEAGDAYKDIDEVIAATELAGISKPVARFTPIGNVK
                     G"
BASE COUNT         1635 a         2125 c         2160 g         1638 t
ORIGIN      
        1 attgagatga aaccggccga aatttctacg atgtcgaaac cgagcttttt gcattcggcg
       61 acgtattgcc gcacggcctc gctgccttgg gcgagcacgc gctcgataaa tccacccgtc
      121 gaaacgagga cgtcatattt gtgacaaaga tcgacaatct cttccacagc cttctccggc
      181 atgagcgtga acgagccgcc ggcaaacttc agtgaatcca cgtagctgcc catcgtttcg
      241 aacaagtctt ccaggtaacg ccggccgact accgagtaat agggcccgcg gatttctgtt
      301 aagccgtgct tccgcggctt acgttcgcgc tcgttgattc ggagaaacgg aaaactgcgc
      361 tcggtacgtt tcgatttcat ctgtccttaa ataaggaacg gaggccgaag cgggaacggc
      421 cgtctgggaa aaggtcgtcg cggcgacttg gttgcggtgt ctcaccgcaa agattttatt
      481 tatgccggga gaacggttga gagcgtgact tcgctgtcga tggtttgctt aaacacgttc
      541 tcgacagtcg cgccgatttt cgatccgtcg cgatcgacga tccagacctg ctgaccttgt
      601 tcgaacggac cgcgcgccat cactttgagt aggatgccct cctcggtggc cggattatcg
      661 agcacctcga cgcagattgc ttcggtcttc ccgaaatccg ccagtaacgt gagtttatcg
      721 tctattttcg gttttttcat tgcgagccag catcgacgtg tggcgtaaaa ctaatcgccg
      781 ctcgcgcgct tggcgagcgt gtccgcgctg ataaacgaat catttaacaa atggcgcgtt
      841 atcgcattac tcagatcgaa gattacgagc agtttatcgg acgggagcag gttcagcgca
      901 ttcgcgagaa cgcccaaagg ttcaaaggca cgcgcgtggc gaacttcaat tccacttatt
      961 acggcggcgg ggtggcggag acactctcat cgctcaccct gctcatgaac agcctcggtc
     1021 ttcgcaccga gtggcgcgtg atcgagggta cgcccgattt tttctcgatt accaagaaga
     1081 tgcacaacgc gctccaaggc ggcgaaatcg acctgtccag catcaaaaag gagatattcg
     1141 agcaggtgat ttatgagaac agcgtgcgca attttctcga gcacgatttc gtgatcgtgc
     1201 acgacccgca gccggttccg ctgattgagc attatgaaaa aaaatgtccg tggatctggc
     1261 gctgccatct cgacctttcc cggccaaacg ccgagacgtg gaaatatctg ctgggttgga
     1321 tcgacaatta cgacgcggtg attgagagct gtcccgaata caaccaggag atgaaaccgc
     1381 cgcagcgcgt tttcatgccg gcgatcgatc cgttcacgat caagaatcgg caactcagcg
     1441 accaggaaat cgacgaacgg ctcgcgcact acgagattcc gaccgacttg ccgttggtcg
     1501 cacaaatttc gcgcttcgat ccgtggaaag atccggaagg tgtggtgaaa gcgttcaagc
     1561 tggcgcgtaa gcaggtcgat gccaggctgg tattgcttgg caacttcgca acggacgacc
     1621 ccgaaggcgc aagaattttc gaaacgctgc gcgcatgcca ggacgagagc atcctgattt
     1681 taaccagcgg cgatgacacc gccctggtga acgcgctgca aacgcgggcg gcggtcgttt
     1741 tgcagaaatc gttacgggag ggattcggtc tcaccgtcgc tgaagcaatg tggaaaggga
     1801 ccccggtaat cggcggcaac gtcggcggca tccgttatca gattgagaat ggcgtcaacg
     1861 gttttctcgt ttcatcgatc gacgaggccg ccgaaagaat tgtgcggctg ctcaaggatg
     1921 agaagttgcg ggaagaaatg ggacgcaagg cgcgcaaaag cgtgcgcgat aaatttctgc
     1981 tcacccgcta tttggaggag tacctcgatc tcttcggcga attcgataaa gatttccgct
     2041 tgcgagaata aaattgtcgc cgagatcggc tatgtatttt cgcgtcgaag gtgcagcctc
     2101 aatatcagcc tggggcaagg ccccaggaaa taaaatagac attgagcgag cgctgaaggc
     2161 gcgtctcaac aacttcggtg gtcgtaacaa tcgggcggcg tggtaccgtt cgggaccgcg
     2221 ggcgtgactc gcggtttcag cacttgtggt tatttgtatt cccatttctg gggcgttgcc
     2281 ccaggctgaa caatgagacc gcgccactgg cgctaaacag ggtacgggat cggtaatccc
     2341 gcctcgattc caagaacact tcaacgccgc tgggagcagc gatcccggct acaaactact
     2401 gaccctttgc aatcccgatt acgccacatg ccacccggcc tcccgcgtca cctgttggtt
     2461 gggatttcag atcatcggct ttttcgtgaa cgataacgga acgcccgatg accgaccgct
     2521 ggtcatttgt cagcgagata tggtgatcca catagtcgag ctttgcatta cctgacgcgt
     2581 cggcttcgat attgcccatg tcacccacat gccgcgcact cgcatcgggg ccagcgtgcg
     2641 gttgcttggt gggattgaaa tgggcgccag cggaggtcgc atccgccgcg ctgcaatcgc
     2701 caaattcatg gacatgaaaa ccatggttgc ccggagtcag ccccgtgatt tccgccttga
     2761 tctgcactcc atcggcttcc tcggtgaaaa tgactttgcc gctcaccttg ttgcccccgg
     2821 taggatgaag catggccaca gctttcagcg gggcagacga tttttctccc ggcgtctgcg
     2881 cccgcgccgt gacggtcgta agtgacaaaa gtatgagggc gactaaccag cttggaaacg
     2941 aattcatatt aatagttcta ttagttttat ttcgtagcgc gggggcagtc cagccgcatg
     3001 atctcgctaa ttcttattgg ctccttatcg aagaatcagc ttcatcccgt cgcaggcaat
     3061 ggacgcttcg acgccgcgct cggctgccat tgcatgaact ttggccaaaa tcttgcgctc
     3121 gtcgcccgag acaatctccg agccgcaatg agtgataatt gcccgcggca cgccctctct
     3181 cgcgcaccac gtcagttggg tgcggaccgg tgcgtgtccg atcagatgtt ttccgcgctt
     3241 gcgaatgaac gagcgggtga gcgttgcgcc atctcctatg taaatttgca cgcctttcag
     3301 cgcggctgcc cggtcgcgga tgaagatgag atcgggcgcg tagaaaatgc gggagcgacc
     3361 tgcgctcaca cgataaccga ccgccggcgc cagaatcgag tgttcgaccg gcaacgcctc
     3421 gaacgtgatg ccacagattt ttgcgggcat acgctctttg atcacacgtc gatcttcgac
     3481 gctacaacct tgcagtgttt gccaggtttt ctgcggcgca tgcaccgggc agggtgcgcc
     3541 atccttcaaa ccccaggcgt ggtccggatg cgcatgcgta agcacgatcg cgcagggatt
     3601 cagccgctca aatttcccca gccaatcaag gccgcagtca atcatcacgt ccgcgccacg
     3661 atgggaaacg agcagcgaag tgtgcatgcg atgccgccgc gtccgcgctt cgatctcgcc
     3721 gcgcgtgccc aggaaggtga gtttcaccgg acaagatgaa tcagtgcgtg acgcaagcaa
     3781 tcctgtctgc gagaccaatg cgcgctcgct ctcctcgtta aaagggaacc ggcgtaggtc
     3841 gagcgtgcga ttccttcaaa aggaaaaggt gcggaaggtc ttatgcgacg tcgaaaattt
     3901 ttatcacacg tattgctgac aacgctgttg atttgcgcgg gcgcattttc ggctgtggcg
     3961 caggaacctg cgcgcacgtc gcaaggagcg atcgcgagtt tcgccccgat catcgagaag
     4021 gtcgcgccga ccgttgtaac agtctttacc actcaaagcg tgtcgcagac cgttttgccg
     4081 tttccgtttt cggacgatgc gttgcgtgag tttttcggcg ggcaacttcc gcagaggcaa
     4141 ggcaaacaga cgctcgaagg actcggctcg ggtgtgatcg tgagcgcgga tggctacatc
     4201 ctcaccgcca accacgtcgt tgcaaaagcc gacgaaatca tggtgggact cggcgccgaa
     4261 ctccgcagat acaaggcgaa caaagtcgga accgatccag gcaccgatct cgcattgttg
     4321 aaaatcgacg agaaaaatct gccggcgatc acgtttgccg acagcgaaaa agcacgcgcg
     4381 ggcgatgtcg tgctggcgct cggcaatcca tttggcctgc ggcaaacggt caccatgggg
     4441 atcatcagcg ccgtcggccg cggcggcatg ggcatcgtcg attacgaaaa tttcatccag
     4501 accgacgcgg ccattaacat gggcaactcc ggcggcgcgc tggtcgatac cgagggccgg
     4561 ctcctcggca tcaacaccgc aatttttacc cgcactggtg gaagccaggg cgttggcttc
     4621 gccattccgt cgaatctcgc gcgcgacgtc atgcagagcc tgcgcgaaaa gggccgcgtc
     4681 gtgcgcggct acatcggcac atcggtccag acgctcaccc cggagctggc cgacgcgttc
     4741 aagctcaaag aactgagtgg cgcgctggtg ggcgaggtca gcccgaaaag tcctgcggag
     4801 aaagcaggga tcaaaagcgg cgacgtgatc acatcggtta acgggaagaa aatcggcgat
     4861 gcccgcgagt tgcgtttgat gattggctcg atggcgccgg gaacaaaggt gaaaatcgag
     4921 gtgaatcgtg aaggtcagaa gaaaacattc ggggtcgatc ttgccgagat gccggctgcc
     4981 gcggccgagc cggcgcccga agcctcacct gaacaaggcg cggagcctga aaaatctacc
     5041 gttttcggcg gtatcgtggt tgccgacgtc accgccgatg tgcgagaggc gttgactttg
     5101 ccgaaggaga ttcagggcgc cgtcattgtg gcgatcgaca ccgcatcgcc tgctgcacaa
     5161 gccggcctgc gcatgggcga cgtgatccag gaagtgaata aacaacctgt taaaaacgcg
     5221 aaagacctcg tcgcgctcag caataaattg aagccgacag aaaaaattct gatgcgcgtc
     5281 tacagccagg gccgcagcgg ctacgttgcg ctggaaccga agtaatgcag gcaagattgc
     5341 ctgcgtcacg cgctcaaata tcgagtatca cgctcgcctt ccattggtcg tcagttttct
     5401 cgacacgaaa ttgatgaagc gtgactgctt ttacgtcggc acgctgctcg tgacggccgg
     5461 gatccaactt ctcgcccgcg actgtggtct tcaaagaata ctgaccgtca ttctcgtcga
     5521 tcttcgcttc tcggacgcgc agcaagagct gctctgcatc tttgaagaag atcagctcct
     5581 gcaaaaaatc gaatagcagc atatcgatcg cgtcattctt caattcgatg cgccgcgtct
     5641 cgcgcggctc gatcgcgtcg aggttttcga tcatcacgtt catcgtcgcg tcggcggcgg
     5701 cgacaaacaa ttcaggcaga ccgcgcccgg tcgcttcaaa cgcgatgtca gcggttccga
     5761 tttcttcgag gtagcgataa ggcatgtgcg gataatgacg aaatccgaat gacgaatgac
     5821 gaatcaagtc cgaaatgacg aaatccaaac aatgtggtga tcgcaccgca cttcttcgtc
     5881 attcatgctt cgacattcct tcgtccttcg tcattcgtgc ttcgtcattt ttaacggtgc
     5941 attcattgag cgcgcggagg atcgcttcct tttcggaaag aacgttcttg ccgataaaaa
     6001 tgagcgcggt ctcggccgac tcaaacggct ccagttccca gcgtcccacg acgaaattaa
     6061 aaagctgcgc tccatcggga aagcgcacga aacctttcgc gcgatgcacg ctcttgggca
     6121 gagcatccgc gaagcgttcg aagcaatcgc gatcaaaagt tttatcggac tcgaacgtga
     6181 atgactcgaa ctccgactga tgcttgtgct cgggtggtgc gacctttttc tcgcgtccga
     6241 ccccgaacaa caatccggga tcgatccggc agcgctcagt gcgaacaatc gccgcagtcg
     6301 aattgatttc gcgcagtttc gcttcgagcg ggtcgatctg cttcggctcg acgagatcga
     6361 ttttgttcaa aagcaaaatg tctgcgccct cgatttgcag ccgcgtggta tgtccgagct
     6421 cggggaagcg aaccagcatg tcggcgtcga tcaccgaaac gacgccgtca agccggcatt
     6481 gcggcagcgc ctcctggatg ttgaacacga gcgcttccgg ttcggcgagg ccggtggtct
     6541 cgacgataat catctctggc gcgactttcg cgatgatttc gttcacagca gcctcgaatt
     6601 cgccgagcaa tgagcagcaa acgcagccgc cgccgagctc ggcgatacgc acatttttcc
     6661 cctcgaccac tttcgtgtcg atggcgattt cgccgaattc gttcatcacg atcgcgatct
     6721 tggccggacg tgtcgcgagg atgtgccgaa gcagcgtggt cttaccgctg ccgagcggac
     6781 cggtgacgag agtgatggga gtgcggagca tgttaaatga cgaaatccga atgacgaagc
     6841 tatcaaccta gatgctttcc gattttcgtc attcgtcatt cgtgcttcgt cattcaattc
     6901 atcctttcac atttccgatc ggcgtaaatc gcgcgacggg tttgctgatc cccgccagct
     6961 cggtcgcggc gatcacttcg tcgatgtctt tgtaagcgtc gccggcttcc tcggcgagac
     7021 cgcgcatgga tgtcgtgcgc acgtagatgc cgcgcgcttc catttcatgc agcaactttt
     7081 ggccgcgcca ttgtttgcgc gctttggtgc ggctcatggt gcggccgctg ccgtgcgcgg
     7141 tgctgaaaaa agtttggtcg ccccctggag cgccaaccaa caggtatgag ccggtctcca
     7201 tgctgccgcc gataatcacc ggctggccga tgtcgcggta gcgcacaggc acttcgtggt
     7261 ggcccggccc gaaagcgcga gtcgcgcctt tgcgatggac gagcagcgtt ttttcttttc
     7321 catcgacagt gtgtttttcc agcttcgcgg tgttgtgcgc gacgtcgtaa accatgtgca
     7381 tttcgagatc ttcggcggag cgtccgagga tttctgaaaa aacttcgcgg atgcggtgca
     7441 ggatcacctg gcggttcgcg aaggacatgt tcagtccgca cttcatcgca gcaaaataat
     7501 cgcgaccttc cggcgaatcg aacggcgcgc aggccagctc gcggtcgagg actttgat
//
LOCUS       DASQUQ010000059         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_644718, whole genome shotgun sequence.
ACCESSION   DASQUQ010000059 DASQUQ010000000
VERSION     DASQUQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644718"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..>2521
                     /locus_tag="VE486_01665"
     CDS             246..>2521
                     /locus_tag="VE486_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HZA37826.1"
                     /translation="MATVDQANPPQEYPDIIGARPTNPLAANFKPFIPSSVKLPELTW
                     PHLITGTFLGMIFGASSLYLVLKVGLTVSASIPVAVISITIFRLVSKFGFRDANVLQN
                     NIVQTAGSAGESIAFGLGVTMPAIMILGFDLEIARVMLVAILGGLLGILMMIPLRRAL
                     IVAEHGILKYPEGTACAEVLKAAASKESLAVADDTHTTADARIINEETASGGKVIFSG
                     FGIGFLYQAAMGAFKMWKDTPEKIFSAPFKAGSIAAEISPTLLGVGYIIGPRIASIMC
                     GGGVLAYLVLIPAIKFFGEHTTVPIAPGTIPISEMTPNDIRGAYVLYIGAGAVAAGGI
                     ISLFRSIPTIWHGLRGGLADLLGRHGPREHAPRTDRDLSMRAVIIGCLAIIAAIMAVP
                     QLHLQGNFLGAFLIVLFGFLFVTVSSRLTGEIGSSSNPISGMTVATLLLTCLVFLILG
                     WTGPPYFVTALSIGGIVCIAASNGGTTSQDLKTGFLVGSTPKFQQIAILFGALASAIV
                     LGPILLKLNDAATVYVPVAEEAARLRAPAAELGRAEKLIGPQGVSDPNTYRAWQRTDP
                     EGGPAQRYLVNEEGVPIYLVDPGINGTHKVRPDGSTVTKFDAPKATLMSYIIKGILSR
                     KLPWALVLLGVMIAIVLEMSGIPSLAFAVGVYLPLSSSSPIFIGGMVRWLVDRYIRGK
                     FRHKNLSEMEMVAEGDKSPGVLLASGYIAGGALAGIVIAFMAGVPRLVDMSTGIEEWS
                     KASNPFFSGPNANLLALIP"
BASE COUNT          499 a          696 c          717 g          609 t
ORIGIN      
        1 cacgcgttct ctcgcggatt tgttcgttgg cgttcacgat ccggtttccg attttatatt
       61 atatgatcca gggcctgccc cgacccaagc gagcggagaa tagtgtcgaa tgaccaaacg
      121 ggtcaagacg gtctgattca atccagcgta atggacggcg ccattatgca cttcgacacg
      181 ccaaagcgac tgtgtcgcat aaaccgcgct tgcgctttcg gcgggttttc tgaaagctct
      241 tcgaaatggc gactgtcgat caagcgaatc cgccgcagga atatcccgac atcatcggcg
      301 cgcgaccgac caatccgctg gcagctaact tcaagccgtt cattccgtcg tcggttaaat
      361 tgccggagct gacctggccg cacttaatca ccggcacatt tcttggaatg attttcggcg
      421 cttcgtcgct ctatctcgtc ctcaaagtgg gcttgactgt gagcgcgtcg attccggtcg
      481 ctgtgatttc catcacgatc tttcgtctgg tttcgaagtt cggcttccgg gacgcgaacg
      541 ttttgcaaaa taacatcgtg caaaccgccg gttcggcggg tgaatcgatc gcatttggcc
      601 tcggcgtcac catgcctgcg attatgattc tcgggttcga tctcgaaatc gctcgagtga
      661 tgctcgtagc cattctaggc gggttgcttg ggatattgat gatgattccc ctgcggcgcg
      721 cgctcatcgt tgctgaacac ggaattctga aatatccgga aggcacagcc tgcgccgaag
      781 ttctcaaagc ggctgcgtcc aaggaatcgc tcgcagtggc cgacgacact cacacgaccg
      841 cagacgctcg gataattaac gaagaaaccg ccagcggcgg caaggtgatc ttcagtggat
      901 tcggcatcgg gttcctgtat caagccgcga tgggcgcctt caagatgtgg aaggacacac
      961 cggagaaaat tttctccgca ccattcaaag cgggttcaat cgcggcggag atttcaccta
     1021 cgctgcttgg ggtaggctat atcatcgggc cgcgcatcgc gtcgatcatg tgtggcggcg
     1081 gggtgctcgc atacctcgtt ttgattccgg caattaaatt ctttggcgaa cacaccacgg
     1141 taccaatcgc tcctggaaca attccgatca gtgagatgac tccgaacgat atccgtggcg
     1201 catacgttct ttacatcggg gcaggtgcgg tcgccgccgg aggaatcatc agcctgtttc
     1261 gctcaatacc cacgatctgg catggattgc gcggcgggct ggcggatttg cttggacgtc
     1321 atggaccgcg cgaacacgcg ccgcgcaccg atcgcgattt gtcgatgcgc gccgtcatca
     1381 ttggatgcct ggcgatcatt gccgcgatca tggcagttcc ccaattgcat ctgcagggca
     1441 atttcctcgg cgcgtttttg attgtgctat ttggtttttt gttcgtcacg gtctcgtcgc
     1501 ggttgaccgg cgagatcggg tcgtcttcga acccgatttc cggaatgacg gttgccacgt
     1561 tgttgctcac ttgtttggtt tttttgatct tgggttggac cggtccgccc tatttcgtca
     1621 cggcgctctc aattggcggc attgtctgca tcgccgcgtc taacggcgga acgacgtcgc
     1681 aagatttgaa aacgggtttt ctcgtaggat cgaccccgaa atttcaacag atcgcgattc
     1741 tcttcggggc gcttgcttcc gcgatcgtcc tcgggccgat cctgttgaaa ttgaatgatg
     1801 ctgccacggt ttacgtccca gtggccgagg aggcggcgag attgcgagct ccggcggcgg
     1861 agctgggccg cgccgaaaaa ctaatcgggc cgcagggcgt ctcagatcca aacacatatc
     1921 gcgcctggca acgaaccgat cctgaaggcg gtcccgccca gcgctatttg gtaaatgagg
     1981 aaggcgttcc gatttacctt gtcgatcccg gcattaacgg cacccacaaa gtccgacccg
     2041 acggatcgac tgtgacgaaa ttcgacgcgc cgaaagcgac gctgatgtcg tacatcatca
     2101 aaggaattct gagccgcaaa ctcccgtggg cgctggtgtt gctcggcgtg atgatagcga
     2161 ttgtcttgga gatgagcggc attccatcgc ttgcctttgc agtcggcgtt tatctcccgc
     2221 tctcatcttc cagcccgatt tttatcggcg gcatggtccg gtggctggtc gatcgttaca
     2281 tacgcggcaa attcagacat aaaaatttga gcgagatgga aatggttgcg gaaggcgata
     2341 aaagccccgg agtcctgctg gcttccggtt acatcgccgg cggcgcactt gccggcatcg
     2401 tgattgcgtt catggccggt gtcccgaggc tggtggatat gagcacgggc attgaagagt
     2461 ggtccaaagc gagcaatcca ttcttcagcg ggccaaacgc caatttgctc gcgctgattc
     2521 c
//
LOCUS       DASQUQ010000060         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_99938, whole genome shotgun sequence.
ACCESSION   DASQUQ010000060 DASQUQ010000000
VERSION     DASQUQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99938"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VE486_01670"
     CDS             <1..717
                     /locus_tag="VE486_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZA37827.1"
                     /translation="TLFESELFGHVKGAFTDAKTDRAGRFELADESTLFMDEIANIPL
                     AQQAKLLRVIETGDFERVGSSKTLHANVRIISATNSNLDDEVTAGRFRQDLLFRLNTI
                     QIALPPLRDRREDIMLLANSFLRQHAQRYRKQMSGFDETARERLLQHRFPGNVRELDH
                     VIERAVLMAQDRQIRAADLGLTTDGGDSRNLEQMSLEEVEQFLIKKALARNDGNARKA
                     AEALGLSRSAFYRRLQQYGL"
     gene            889..2223
                     /locus_tag="VE486_01675"
     CDS             889..2223
                     /locus_tag="VE486_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZA37828.1"
                     /translation="MNRPPKSRYRPSHEGKLTWLTFGAAAPAVVVALALLWFGDYSAK
                     VQWTLTILVVACFLLFISSARDHIVHPLQTMSNLLAALREGDYSIRARGASGDNALGE
                     VLLEINSLGETLRRQRLGAFEATALLRTIMGEIDVAVFTFDPERRLRLINRAGETLLG
                     QPIDKLLGKGAKELGLDAFYEVDQDEPLTLSFPGGAGRWGIRRSTFREQGLPHDLLVL
                     TDLSRTLREEERRAWQRLVRVLGHEMNNSLAPIKSLAASLESLLRRDPPPPDWRDDAR
                     SGLSSIASRADSLSRFLQAYTRLTKLPPPQKRDVEIGELVQRVADLEPRLSVKILPGP
                     KTRIRADAAQIEQLLINLVHNAVDAALETHGDVAIGWREAGDSVEILVQDEGPGIMNP
                     ANLFVPFFTTKPDGSGIGLPLSRQIAEAHGGSLVLMNRPAGTGAEALLRLPR"
     gene            2450..2522
                     /locus_tag="VE486_01680"
     tRNA            2450..2522
                     /locus_tag="VE486_01680"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2483..2485,aa:Phe,seq:gaa)
     gene            2548..>3253
                     /locus_tag="VE486_01685"
     CDS             2548..>3253
                     /locus_tag="VE486_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZA37829.1"
                     /translation="MKDWTDGGEEWSEPWGNSAAQWAGVIFPRIRDWLPAGTILEIAP
                     GFGRWTHYLKKHCDNLWLVEKADQCFEACCRRFGNEPRIKVYHNDGHSLSMLPDSSID
                     FVFSFDSLVHDRRGFVESYLRQLGSKLKVGGKGFFHHSNLGAYADSVRERRPTRVVKL
                     LEKARILDLQHHRTPTMSADLFRALCEQNGLHCVRQELINWRARRLIDCFSFFVRDRS
                     QQTKPTEIIRNPNFMRE"
BASE COUNT          692 a          878 c          964 g          719 t
ORIGIN      
        1 accctgtttg agagcgaact ctttggacat gtaaaaggcg cattcaccga tgcgaagacg
       61 gatcgggccg ggcgatttga gcttgcggac gagagcacgc tcttcatgga cgagatcgca
      121 aacatcccgc tcgcccagca ggcgaaactg ttgcgtgtga tcgagaccgg cgattttgag
      181 cgggtcggct catcgaaaac gctgcatgca aatgtgcgca tcatttccgc tacaaactca
      241 aatctggatg atgaagtcac ggccggacgt tttcggcagg accttctctt tcgcttgaat
      301 acaatccaga ttgcgttacc gccgttgcgc gaccggcgcg aggacatcat gctgctggca
      361 aacagctttc ttcgacagca cgcgcaacga tatcggaaac agatgagtgg tttcgatgag
      421 acggcgcgcg aacgtttgct gcaacaccgc tttcctggaa acgtgcgcga gctcgatcat
      481 gtgatcgaac gggccgtgct catggcgcaa gatcggcaaa tcagagcagc cgatctgggt
      541 ctgacaacag acggagggga ttcgcgcaat cttgagcaaa tgagtctgga ggaagtggag
      601 caatttctaa ttaagaaggc gctggcgcga aatgatggca atgcgcggaa agcagcggaa
      661 gcgctcggat tgagccgaag cgcattttat cgccggttgc agcagtatgg actctgaccg
      721 ggtagcgcac gcgtctcacg tgctggcgat tgcgtcctcg cgatcgcgaa cttccctttc
      781 actcctatct ttcagcgcaa tcgctgatca aagtgaagtt cgttttggcg agacgcccaa
      841 accagcacgc gagacgcgtg cgctacccgg acaggaaggc gagcgtccat gaatcggccg
      901 ccgaagagtc gttaccggcc ttcgcacgaa ggaaaactga cctggctcac ctttggggcg
      961 gctgctcccg cggtcgttgt tgcgctcgcg cttctttggt tcggcgatta cagcgccaaa
     1021 gtgcagtgga ctctaacgat cctggtcgtt gcttgtttcc tccttttcat ttccagcgcg
     1081 cgcgaccaca tcgtgcatcc tctccaaacc atgtccaatc tgctcgccgc attgcgcgag
     1141 ggcgattact cgatccgggc gcggggcgcc agcggagaca acgcattggg cgaggtcctt
     1201 ttggagatca actcgctcgg tgaaacgttg cgccggcagc ggctgggcgc ctttgaagca
     1261 acggcgttgc tgcgcacgat catgggggaa atcgatgtcg cggttttcac tttcgatccc
     1321 gagcgccgtt tgcgtctgat taatcgcgcc ggcgaaacct tgctcggcca accgatcgac
     1381 aaactgctcg gaaaaggcgc aaaagagctg ggtctcgacg ccttttatga ggttgaccag
     1441 gacgagcctc ttacgctcag ttttcctgga ggcgccgggc gttggggaat tcggcgcagc
     1501 acttttcgcg aacagggtct cccgcacgac ttgcttgtgc tgacggatct cagtcgcacc
     1561 ctgcgcgaag aggaacggcg cgcctggcag cgattggttc gcgtgctggg tcacgagatg
     1621 aataactcgc tggcgccgat caaatcgctt gcggccagtt tggaatcgct cctgcgacgc
     1681 gacccgcctc cgccggattg gcgggacgat gcgcgcagtg gattgagctc aatcgcgtcg
     1741 cgggcggatt cgctcagtcg cttcctgcaa gcctatacgc gtctcacgaa acttccgccg
     1801 ccacaaaaac gagatgtaga gatcggtgaa cttgtccagc gcgtggccga tttggaacca
     1861 aggctgagcg tgaaaatcct acctggcccg aaaacaagaa tccgtgcaga cgcagcgcag
     1921 atcgaacagt tgttaatcaa tttggtgcac aatgcggttg atgccgcgct cgagacgcac
     1981 ggagatgttg caatcggatg gcgcgaggca ggcgattcgg ttgaaattct tgttcaggac
     2041 gaagggccgg gaattatgaa tccggcgaat cttttcgttc cgttttttac aacgaagccc
     2101 gacggctctg gaattggtct tccgctgagc cgtcaaattg ccgaagcgca cggaggttca
     2161 ttggtgctaa tgaaccggcc cgccggcacc ggcgcagaag cgttgctgcg actcccgcga
     2221 tagaggccga tttttggaat gcggcgacat gtcgccgctt taaaagcgcg gaaggcgtct
     2281 gttctgcttt gcggaagctg ggagtgggca ctgcctgacc tgtgcgaagc cgaatgagcg
     2341 tcctagcgtc cctttggcaa tttcgaaatg acaaacgttg agggttacgc gttttctgct
     2401 ttactgacaa cccgcagcgc gccttgcacg cgagatcgac atctgcaacg gctgggtagc
     2461 tcagttggca gagcagtgga ctgaaaatcc acgtgtcggc ggttcgattc cgcccccagc
     2521 cagatttttc agacacgttc aaatttcatg aaggattgga cagacggcgg ggaggaatgg
     2581 tcggaaccat ggggaaattc cgcagcgcaa tgggccggtg tgattttccc gcggattcgc
     2641 gattggctcc ctgccggcac aattctcgaa atcgcccccg gttttggccg gtggacgcat
     2701 taccttaaga aacattgcga caatttgtgg cttgtggaga aagccgacca atgtttcgaa
     2761 gcgtgctgcc gccgtttcgg taacgagccg cgcatcaagg tttatcacaa cgatgggcac
     2821 tcgctgtcga tgttaccgga cagctcgatt gacttcgttt ttagttttga ctcgctggtc
     2881 catgacaggc gcggtttcgt cgaatcgtat ctacgccaac tgggatcgaa attgaaagtc
     2941 ggcggaaaag gtttcttcca tcattcgaac cttggcgcgt atgcggattc agttcgcgag
     3001 cgccgcccaa cgcgcgtcgt taagttgttg gagaaggcca gaattctcga cttgcaacac
     3061 catcgcacgc caaccatgtc tgccgatttg tttcgtgctt tgtgcgagca aaacgggctg
     3121 cattgtgtga ggcaggagct gatcaactgg cgtgcgcgtc gattgatcga ttgcttttcc
     3181 ttcttcgtgc gggaccgatc tcaacaaaca aaacccactg agataatccg aaatccgaac
     3241 tttatgcggg aag
//
LOCUS       DASQUQ010000061         5392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_645241, whole genome shotgun sequence.
ACCESSION   DASQUQ010000061 DASQUQ010000000
VERSION     DASQUQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645241"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..1384
                     /locus_tag="VE486_01690"
     CDS             530..1384
                     /locus_tag="VE486_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZA37830.1"
                     /translation="MATNPTWKLGGDLIVNRLGFGAMRITGEGIWGWPTDRNNALKIL
                     RRAVELGVNLIDTADSYGPETSELLIAEALHPYPAGLVIATKGGLTRPGPGQWVPNGR
                     PEYLKRCVEGSLKRLRLERIDLYQLHTIDPKVPTEESLGALKEMQNAGKIRHLGLSNV
                     STAEIERARKIVPIMSVQNRYNIEDRESEKVLVYCEKENLGFLPWFPIGGGNGLPAEN
                     ALKTAARDHGMSAIQIALAWLLERSPVMLPIPGTSSIAHLKENVAAAKIKLTSAEWKA
                     IDALAGQG"
     gene            1501..2649
                     /locus_tag="VE486_01695"
     CDS             1501..2649
                     /locus_tag="VE486_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZA37831.1"
                     /translation="MNGVGLNIRSVTLPGDLIALSSISEFVFYTFAFILGAAVGSFLN
                     VCIYRWPVDLSINRPRRSFCPVCKAPIPWHQNLPLISWLFLRGRCANCGSKIAFRYFA
                     VELLTALLFLAIWQSFPWEMAIAYWVFVSLLIVGTFIDFEHFIIPDQVTIGGIIAGVL
                     ASVTVPALMDTNSRLAAGVRSILAAALGYVILLIVLEAGKIAFGRKRIRFDSPTPFTW
                     TKRDDDADLVVGSEKSLWSDHFAREKDRLLLDCDEAKIDNHTYGGVTLEFYYDRVTVE
                     GNVLELDHVTQISGVARGLLIPREAMGRGDLKFLAAIGAFLGWRAVLFSLFAGSLLGS
                     VIGLITLIAGKRVWSAKLPFGPYLAFGAVSWMFFGDLFLRWYAGLINP"
     gene            2758..3138
                     /locus_tag="VE486_01700"
     CDS             2758..3138
                     /locus_tag="VE486_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZA37832.1"
                     /translation="MHPSLDQASLEFITWTIPLLERLSASTSVRNQLDRASTSVPLNI
                     AEGNGKFTSPDRCRFFDNARGSALESAACLDMMVAKKFFSAAEIRAGKAMLVKIVSML
                     FGLIRANSDVRVFEQPASYLAEKE"
     gene            complement(3196..4830)
                     /locus_tag="VE486_01705"
     CDS             complement(3196..4830)
                     /locus_tag="VE486_01705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZA37833.1"
                     /translation="MRWEVDVISGGRSPLELPLGTGESIISVAMNFEYDVVIIGGAFS
                     GAATALMLKRKRPEARVLIIEKTAEFDRKVGESTTEVSSCYMTRILGLTHYLGHHQLA
                     KQGLRLWFSNRPDQLFEDCVEIGTRYQSRLPGFQVDRAKLDSHLLDLAVEAGCDLWRP
                     AKVTKCELNGANGQAVSAVVDHTERSVTCRWVVDATGRATMLARKLGHFRPNTEHPIN
                     AVWARFTGVKQWDSYEWRERFPDYANHCRTGREWATNHLFGRGWWVWIIPLRGGDVSA
                     GIVYDNRIFKLPEGPNLAQRLHAHIAGHPVGREIFGAAKAIEGDVHALSMLPYHSEKV
                     CDDGWAAVGDATGFIDPLYSPGLDFCSYTSYYVADLLARSLAGEDVEERLRNYNRQYP
                     VTYRYWFETLYKDKYYYMGDADLMSAALLLDVSSYYIGLVGAVYRDPEREFLNLPFSG
                     FGGRLARNTMSFYNRRLVALAKRRWATGYYGKRNSGWRELYDGFVPDIRVRKRIWLGL
                     GRWWKCELINLGLMLRRQTENSASQASTTPAEACEV"
     gene            4988..>5392
                     /locus_tag="VE486_01710"
     CDS             4988..>5392
                     /locus_tag="VE486_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37834.1"
                     /translation="MNVEFSGTFRRLDGVSPYQHMILTNARLIFPDGIRDGLEVVVEG
                     GRITAIRPPSPGYGAAGEQTRTTGKDLIDLGGNYLAPGFVDLHVHGAVGRDTMEASPE
                     AFGVICDYHASGGTTSLLLTTATAPMNSIVDVL"
BASE COUNT         1178 a         1506 c         1489 g         1219 t
ORIGIN      
        1 aagctgtaga ggcagccgtg ccgcctgcag ttgtctaatg ttgcgcccgg aaaaagtgat
       61 agctagggac tgtgttggtc agcgccgaat tcgtgatgat tctgcttttg aatggtccgg
      121 caaaggcaaa ccctgtggca accaggagaa acaatgtagc ccaacatcga ccgatcagga
      181 ttttcattgt ttgagatata gcgatcaacc tgcgggccat gaagctaaat cttagaccgc
      241 taaatcagat tgaacgggcg tcatgcgtcc gttacaaaag tgcgcagcca ttctttgcag
      301 ccggttcact ctcaacggcg agagcgacag cgcacaagag cgcgcttgag tcggcagcag
      361 ttaacgtaaa cgtacgtgga agctatggcc agcacatctc gcgaatcgga gcaaacgccg
      421 ggggctgacg cgccgaatgt ttcttggcgc gactctggct ggcggagcgg cctttttggc
      481 gggaggatcc gatgtaatct tcggagctca cgcttcacca tcaccgacta tggccacgaa
      541 tcctacctgg aagttgggcg gcgatctcat tgtcaatcgc ctcggttttg gtgcgatgcg
      601 catcaccggg gaaggcattt ggggttggcc gaccgatcgc aacaacgcgc tcaaaattct
      661 gagacgcgcc gtcgagctcg gtgtgaactt gatcgatact gcagactcgt acgggccgga
      721 aacaagcgag ctgctgatcg ccgaagcgct tcacccttat cctgccggcc tcgttatcgc
      781 gaccaagggc ggtctgaccc ggccgggacc cggccagtgg gtgccaaacg ggcgacctga
      841 atatctcaaa cgctgtgtcg aaggtagtct gaagcgactg cggctcgagc ggatcgatct
      901 ctatcaattg cacaccatcg atccgaaagt gccgacggag gaatcgctcg gcgcgcttaa
      961 ggaaatgcaa aacgccggaa agatccggca cctgggatta tcaaacgtga gcacggcgga
     1021 aatcgagcgg gcgcgcaaga tcgttccgat catgagtgtc cagaaccgct acaacattga
     1081 ggaccgcgaa tccgaaaaag ttctggtcta ttgcgagaag gagaaccttg gcttcctgcc
     1141 gtggttccca attggcggag gcaatggttt gccagctgag aacgcgctga aaacggcggc
     1201 gagggatcat ggcatgagcg ctatccagat cgcgctcgcc tggcttctgg agcgatcgcc
     1261 tgtgatgctg ccgattccgg gcacatcctc catcgcgcat ctcaaggaaa acgtcgcagc
     1321 cgcgaagatc aaattaactt cagcagaatg gaaagcgatt gatgccttag cgggtcaggg
     1381 ttgacactcc tggccggaaa gcaaggcgac ccgccaccga tgttaccttc gcgcagatca
     1441 gccgctgcgc ttgcgaggat ttgaaatcgc atccgcgctt ggtaaaggaa gctacttttc
     1501 atgaacggcg ttggactcaa catacgcagc gttacgctgc ccggagattt gattgcgtta
     1561 tcatccatct ccgagttcgt cttctacacg tttgctttta ttctcggcgc cgcggtcggc
     1621 tcgtttttaa acgtctgcat ttaccgttgg cccgtcgatc tctcgattaa caggccgcgc
     1681 cgctcttttt gcccggtttg caaggcgccg atcccatggc atcaaaatct gccattaatc
     1741 agctggcttt ttctccgcgg ccgctgcgcc aattgcggga gcaagatcgc gtttcgctat
     1801 tttgctgtgg aattgcttac cgcgcttctc ttcctcgcga tctggcaaag cttcccgtgg
     1861 gagatggcga tcgcctactg ggttttcgtt tccctgctta tcgttgggac attcatcgac
     1921 ttcgagcatt tcatcattcc cgaccaggtg acaatcggcg ggatcatcgc gggagtcctc
     1981 gccagcgtga cggtgccagc tttgatggac acgaattcgc gcttggctgc gggtgtccgc
     2041 tccatcctgg ccgctgccct aggctacgtg atccttttaa tcgttctgga agcgggcaaa
     2101 atcgcttttg gaagaaaaag aattcgtttc gattctccca ccccgtttac ctggacaaag
     2161 cgtgacgacg atgccgatct cgtggtcggt agcgaaaaaa gtttgtggag cgatcatttc
     2221 gcgcgcgaga aagatcggct gttgctcgat tgcgatgagg cgaagatcga caaccacact
     2281 tatgggggtg tgacgttgga attctattac gaccgcgtca cagtggaagg caacgtcctg
     2341 gagctcgatc acgtaacgca gatatcgggt gtagcgcgtg gcctgctgat cccacgcgaa
     2401 gcgatgggac gcggcgacct gaagtttctc gccgctattg gcgcattcct cggatggcgc
     2461 gcggtcttgt tcagtctttt tgccggatcg cttctcggtt cggtcatcgg cttgattacg
     2521 ctcatcgcgg ggaagcgtgt ctggtcggca aagctgccgt tcggacctta tctcgcgttc
     2581 ggcgcggtaa gctggatgtt ttttggcgat ctttttttgc gctggtacgc cggattgatt
     2641 aatccttagc ggcgaagaag gaacgagcga gattccgaat aggaatggcg gataaaacgt
     2701 gaccaagcag ccgttaaaat cttaatctta atcgtattcc tattcctaac tcttccaatg
     2761 cacccgtccc tcgatcaagc ctccctggag ttcatcacat ggacgatccc tctcttggag
     2821 cgattgtcgg cgagtacatc cgttcgcaat caactcgatc gcgcttcgac gtctgttcct
     2881 ttgaacatcg cggaaggaaa cggaaaattc acttcgcccg accgctgccg attcttcgat
     2941 aacgcccgtg gttccgcatt ggagtcagct gcatgtcttg acatgatggt tgccaaaaaa
     3001 ttcttcagcg cggccgaaat ccgagcgggc aaagcgatgc tcgtaaagat cgtgtcgatg
     3061 ctgtttggac tgatccgcgc aaattctgac gtgcgggttt tcgaacagcc agcgagttat
     3121 ctggcggaga aagaatagga gtaagaatag gaatacgaga taagacagaa ttcgtccgct
     3181 gatatcggga tttgcttata cttcgcacgc ttccgccggc gtagtcgaag cctggctcgc
     3241 ggagttctcc gtttgtcggc gtagcattaa tcccagattg atcagctcgc atttccacca
     3301 acgccccagt cccagccaaa ttcgtttccg aacccggata tctgggacaa atccgtcgta
     3361 caattcgcgc cagccggaat ttcttttgcc gtaatagcca gtcgcccaac gccgcttggc
     3421 caacgcaaca aggcggcgat tataaaaact catcgtgttc cgggccagtc gcccaccgaa
     3481 accgctgaag ggcagattga gaaattcgcg ctccggatcg cgataaaccg ccccaaccaa
     3541 gccgatataa tagctgctca cgtccagcag gagcgcggct gacatcagat cggcgtctcc
     3601 catgtagtag tatttgtcct tatacaaagt ttcgaaccag taacggtaag tgaccggata
     3661 ttgccggttg taattacgaa gccgctcctc cacgtcttcg cccgctaaac tacgcgccag
     3721 cagatcggcg acgtagtagg aagtgtaaga acaaaagtcg agacctggac tgtaaagcgg
     3781 atcgataaag ccagttgcat caccaactgc ggcccagcca tcatcgcaaa ctttttcgct
     3841 gtgatatggc agcatcgaca aggcgtgcac atcgccttca atggcttttg ccgcgccaaa
     3901 gatttcgcgg ccaaccgggt gaccggcaat gtgcgcatgc aaacgctggg cgagattagg
     3961 cccttccggc agtttgaaaa tccggttgtc atacacaatg ccggcactga cgtcacctcc
     4021 gcgcaatgga atgatccata cccaccagcc gcggccaaac agatgattgg tggcccattc
     4081 acggccggtg cgacaatgat tggcgtagtc cggaaagcgt tcccgccatt catagctgtc
     4141 ccattgcttg acgccggtga agcgtgccca cacagcgttg atgggatgtt cggtgttcgg
     4201 ccgaaaatgt cccagtttcc gggcgagcat ggtcgcgcga cctgtggcat cgactaccca
     4261 acggcaagtc acgctgcgtt cggtgtgatc cacaacagca cttaccgcct gcccgtttgc
     4321 gccgttgagt tcgcattttg tgaccttggc cggccgccag agatcgcaac cggcttccac
     4381 cgcgaggtcg agcaggtgcg aatcgagttt tgcccgatca acttgaaacc ccggcaaccg
     4441 gctctgatat cgcgtgccga tctcgacgca atcttcaaag agctgatcgg gccgattcga
     4501 aaaccacagg cgcaatccct gttttgccag ttgatgatga ccgagataat gggtcaggcc
     4561 caaaatccgc gtcatgtaac aactgctcac ttcggtggtc gattccccga ccttacggtc
     4621 gaactcagcc gttttttcga tgatgagaac gcgcgcctca ggtcgtttgc gtttgagcat
     4681 gagcgccgtc gcggcgccag aaaatgcgcc gccgataatt acgacatcgt actcgaaatt
     4741 catcgcgact gagataatgg actcaccggt gcccaaaggc aattccagcg gagaacgacc
     4801 accagaaatc acatcgactt cccagcgcac tccgcttccg ggggcactgg ccgctggcct
     4861 gtagtttcgc ggcagcttgc cgcgacctgc cttttaccgc ttcacgtgtt tatgatgcca
     4921 ggcattcggc cagcggcctg cgctccccag aagcgcattg aaagtgttcc gcgccttgtg
     4981 atgcgggttg aacgttgaat tttctggcac atttcgtcgg ctcgacggag tctcgcccta
     5041 ccagcacatg atcctcacaa atgcgcgttt gatttttcca gatgggattc gcgatggcct
     5101 cgaagtcgtc gtcgaaggag gaagaattac ggcaatacgc ccgccttcgc caggctacgg
     5161 cgcggcaggc gagcaaaccc gaaccaccgg caaagacctt atcgatcttg gcggaaacta
     5221 tctcgcacct ggatttgttg atcttcacgt ccacggcgcc gtcgggcgcg acacgatgga
     5281 ggcatcgccg gaagcgtttg gcgtcatctg cgattaccat gcgagtggcg gcacgacttc
     5341 acttttgctt acgacggcga cggcgccgat gaacagtatc gtcgatgttc ta
//
LOCUS       DASQUQ010000062         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_280935, whole genome shotgun sequence.
ACCESSION   DASQUQ010000062 DASQUQ010000000
VERSION     DASQUQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280935"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..687
                     /locus_tag="VE486_01715"
     CDS             268..687
                     /locus_tag="VE486_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZA37835.1"
                     /translation="MVEETVWRGTSSQWKNFGVYALCALTCWLIVPIFIALARYLQTK
                     CKIYELTTERLKITQGVFSKVTSTLELYRVKDIEMHQPFLARLVGVENVQMNTSDASS
                     PFVFIDAVPSSIGLADKLRNQVEIIRQHKRVREIDIE"
     gene            complement(699..935)
                     /locus_tag="VE486_01720"
     CDS             complement(699..935)
                     /locus_tag="VE486_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37836.1"
                     /translation="MHEPGTNDLGPMRIRALAIGAESVGAFALGAVALGVLALGAVAI
                     GRLVIGRARIKRLEIDELVVNKLQVRHSLETPAD"
     gene            complement(913..1317)
                     /locus_tag="VE486_01725"
     CDS             complement(913..1317)
                     /locus_tag="VE486_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZA37837.1"
                     /translation="MAADVFHGGCLCGGVRYEASGELYNITHCHCEDCRKSAGAPFVT
                     WASFRRNNFRFTHGAPHEIAWAGRIRSFCANCGTALTFMSGPDTDEVDVTVSSFDQPD
                     TTLPQDHVWTEDRLPWVKLADNLPQYARARNK"
     gene            complement(1341..2333)
                     /locus_tag="VE486_01730"
     CDS             complement(1341..2333)
                     /locus_tag="VE486_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HZA37838.1"
                     /translation="MSMLASDLFPSARRALSILLAALLLAGPALGANPPARPDRRSKP
                     PQFEALPLVRSRQNHLLVRAFINGKPAWLTVDSGAPVSAIALHRRDYFRLTPITAKSN
                     LPARVQINGAFNNVAIARKLRIGALTLVDEPVVVVDLGKPSRAAQLRHEPQIDGIIGA
                     DILFPTKAVLDCQRQLLILKIDPDVIGSAPGFDRSGWQAVPIQVSDGFNLYVNGSVNG
                     KRARLMVDTGSFATLLHRSFVRQMRIRTEETAFSSSAVNQKDRGVRVARIRKLSVGSV
                     DIVGRQVGVIDLEGVIHGGLLEGSPPVAGLLGAEILKRHHGIIDFGTRTLYLKK"
     gene            complement(2526..>3582)
                     /locus_tag="VE486_01735"
     CDS             complement(2526..>3582)
                     /locus_tag="VE486_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37839.1"
                     /translation="VYRGLKGGIEKFCQFALPAMAILAMIVLIRVLTLGTPNLARPDQ
                     NVVNGLGYLWNPDFSKLADFDTWLAAAGQIFFSLSVGFGVILNYASYLKKNDDIALSG
                     LTASATNELFEVGFGGLITVTAAFVFLGASGVAGGFGLGFQTLPVVFHQMGFIGRWIG
                     FAWFFLLFLAAITSSISMLQPAKAFFEEALAIPSSKAITLVSIICGLGSLWVIWFSKN
                     TVALDTMDFWVGTFCIFVLATVQIVCFGWIWGVRNGATELDQGALMKIPRFFLFVMKY
                     IAPAYLLVVLIGFSYSNFGAALSKTANDPVALWTLLIIVAVIALLVGVLVVGERRWRK
                     AGLDIDGRMPLKEAPKQ"
BASE COUNT          904 a         1041 c          951 g          686 t
ORIGIN      
        1 cgattaaatg atcgagcgca aaagtgatga gcggtttttg aaagatcggc acgaggggtt
       61 tgggcaattc gtccgtcaac ggccgcaaac gtttaccaag tccggcgccc aagacgaatg
      121 cctgtgtaat ttcgttcatg gggaaaagaa tgaaagattc ctcgacttcg ctcggaatga
      181 caaaggaagc ggccgcaggc gtttgccgag tccggcgcca agaacgaaag cttgcgtgat
      241 actcttcatc gcgagaaaag tgcagtcgtg gtcgaagaaa ctgtttggcg tggcacctca
      301 tcgcaatgga aaaattttgg cgtgtacgct ctgtgcgcgc tcacctgctg gctcatcgtg
      361 ccaatcttta tcgcgctggc gcgctatctc caaacaaaat gcaagattta cgaattaacg
      421 acggaacgac tgaaaattac acaaggcgtt tttagcaaag tcaccagcac gctcgagttg
      481 taccgcgtga aagacatcga aatgcaccag ccgtttcttg cccgattggt cggggtcgaa
      541 aacgtgcaaa tgaatacgtc ggacgcgtca tcaccgttcg ttttcatcga cgcggtgcca
      601 tccagcattg gtctcgccga taagctgcgc aatcaagtcg aaatcattcg gcaacacaag
      661 cgcgtacgcg agatcgacat cgagtagcag cgattgattc aatcggcggg agtttccagc
      721 gaatgtctca cttgcagctt gttcacaacg agctcgtcga tctcaagcct tttgattctc
      781 gcccgcccaa taacaagccg accaatcgca accgcgccta aagcaagtac gccaagcgca
      841 accgcgccga gcgcgaaagc tccaacgctt tccgctccaa ttgccagcgc ccgaatgcgc
      901 atcgggccga gatcatttgt tcctggctcg tgcatactgc ggcaaattat cggcaagctt
      961 cacccacgga agccggtctt cggtccagac gtggtcctga ggtagagtcg tatcgggctg
     1021 atcgaaactg gaaacggtta catccacttc gtcggtgtcg ggtccgctca tgaaggtcaa
     1081 cgccgtaccg cagtttgcgc aaaatgatcg aatgcgcccg gcccacgcga tttcatgcgg
     1141 cgcaccgtga gtaaatcgaa aattgtttcg tcgaaacgaa gcccacgtca cgaagggcgc
     1201 gccggcgctc ttgcgacaat cttcgcaatg gcaatgcgta atattgtaaa gctcgccgct
     1261 tgcttcatag cggaccccgc cgcacagaca acctccgtga aaaacgtctg ccgccatctc
     1321 aaatacttcg ccctgcgcac ctactttttc aaataaagcg tgcgcgtgcc gaaatcgatg
     1381 atgccatggt gccgtttgag aatttctgcg cccaacaacc cggccacagg cggcgaacct
     1441 tccagcaacc cgccgtgaat cactccctcc agatcgatta cgcccacttg tctgccaacg
     1501 atatcgactg agccgaccga taacttgcga atccgcgcca cccgcacacc acgatccttc
     1561 tgatttacag cagaagagct aaacgcagtc tcctccgtcc gaatccgcat ttgccgaacg
     1621 aaggagcgat gcagcaacgt ggcgaaagaa ccggtatcga ccatgagcct ggcgcgcttg
     1681 ccgttgacag agccattgac atataaattg aaaccatcgc tgacctgaat cggcacagcc
     1741 tgccatccgc tgcggtcgaa accgggagcg ctgccaataa cgtccggatc gatttttaag
     1801 atgaggagtt gcctctgaca atccagcact gctttggtgg ggaagagaat atcggccccg
     1861 ataattccgt cgatttgtgg ctcgtgccgc agttgagcag cgcgactcgg cttcccaaga
     1921 tcgacaacaa ccaccggctc atccaccagg gttagcgcac cgatccgcaa cttacgcgcg
     1981 atcgccacgt tattgaacgc gccattaatc tgaacccgcg ccggcagatt cgatttggcc
     2041 gtaatcggcg tgagccggaa ataatcgcgg cgatgcaaag cgatcgcgct caccggcgcg
     2101 ccgctatcga cggttaacca tgccggcttg ccattgataa aagcgcgcac cagcaaatgg
     2161 ttttgtcgcg agcgcacgag cggcaacgct tcaaattgcg gtggcttgga gcgtcgatcg
     2221 gggcgagcag gcggattagc gccaagggcg ggtccggcga gcagaagagc cgcgagcaag
     2281 atcgacaaag ctcgccgggc cgatggaaaa aggtccgaag cgagcatgga catgggcgcc
     2341 gacgatagca cgctttaaca aaacgcaagt agattcggag cgatgaagtc cgaaggtgat
     2401 cggggatcag ggctctcgat cgcgcggctt ttcaggcggc gaaccaagca gacgagagta
     2461 agcccaaatc gtcacgcccc agacgagcgc gagggaacag gacatgaata gccaacccca
     2521 actgttcatt gcttaggcgc ttcttttaat ggcatccgcc catcgatgtc cagcccagcc
     2581 ttgcgccaac ggcgttcgcc gacaacaaga accccgacga gcaatgcgat caccgccacg
     2641 atgatcaaca gtgtccagag cgctaccgga tcatttgcag tcttcgacag tgccgcgccg
     2701 aaattcgaat aactgaatcc gatcagcacg acgagcaggt aagccggcgc gatgtacttc
     2761 atcacgaaca aaaagaatcg cgggattttc atgagcgctc cctggtcgag ctcggtcgcg
     2821 ccatttcgca ctccccatat ccagccgaaa cagacgatct gcacggtcgc gagaacgaaa
     2881 atgcaaaatg tgcctaccca gaaatccatg gtgtccagag caaccgtgtt tttgctgaac
     2941 cagatcaccc agagggaacc gagaccgcaa atgatcgaaa caagcgtgat cgccttgctc
     3001 gatggaatag cgagcgcttc ctcgaagaat gctttcgccg gttgcaacat cgaaatcgag
     3061 ctggtgatcg cggctagaaa cagaagaaaa aaccaggcga acccgatcca gcggccaata
     3121 aatcccattt gatggaagac cactggcagt gtttgaaatc caagaccgaa gccgccggcg
     3181 acaccactcg cgccgagaaa cacaaaggct gcggtgaccg tgatcaaccc gccgaaaccg
     3241 acctcgaaca actcatttgt cgcgctcgcc gttagcccgg agagggcgat atcgtcgttc
     3301 tttttcaagt acgaggcata attcagaatc acgccgaaac cgaccgacag gctgaaaaag
     3361 atttggcccg cggcggcgag ccacgtgtcg aagtcggcga gcttcgaaaa atcggggttc
     3421 cacaggtagc ccagaccgtt cacaacgttc tggtccggcc gcgccagatt tggtgtgcct
     3481 agcgttagca cccgaatcag cacgatcatt gccagtatcg ccatcgctgg cagcgcgaac
     3541 tggcaaaatt tctcgatgcc tcctttcagc ccgcgataga cg
//
LOCUS       DASQUQ010000063        12741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1280840, whole genome shotgun sequence.
ACCESSION   DASQUQ010000063 DASQUQ010000000
VERSION     DASQUQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280840"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1062)
                     /locus_tag="VE486_01740"
     CDS             complement(292..1062)
                     /locus_tag="VE486_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZA37840.1"
                     /translation="MSTAKKSSPSTREMERINWIEFREWVPAKIRTVLLPLGTLEPHG
                     VAPNGTDILAPVAMAHEIAPRVNALVAPAIPYGFTGVLDAYPGSFTIPEAAFRDYVHA
                     VLAGLAKNQFKNIILLNGHGGGQTAVLSALAQEVGRETVTRILVLNWWSYCSDVTLEV
                     FGEHGGHGAENETAYIMAIDPALIQKKHYKPEMVTALPQPNTWSAYPFPSTIMLYKEG
                     QGYPKFDLVKAKIYFTKVNDKIAGLIHETIRKWDMAGV"
     gene            complement(1136..2392)
                     /locus_tag="VE486_01745"
     CDS             complement(1136..2392)
                     /locus_tag="VE486_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZA37841.1"
                     /translation="MIIRARIVVTMDGPPIENGAIAISKNQIIDVGKFPEISARHSGL
                     EVVDLGERALLPGLINAHCHLDYTCLRGKIRPQKSFTDWIRAINTEKSKLSRQDYLVS
                     IAEGFAEAKRFGTTTVANLTAFPELISEIQTPIRTWWFAELIDVRSPERANEIVDRAI
                     ESLEPAENRGLAPHAPFTASSNLYRLCEEVARRQSILLTTHLAESREELEMFRNQSGP
                     LYEFMKSIGRPMDDCGQETPLAFFANIAGAPPSTSLRAGSGRALPNEWIVAHLNELAE
                     SGFKLLKESTNKFHLVHCPRSHDYFGHSRFRFEKLRGLGFNICLGTDSLASNEDLSLF
                     AEMRAFQRSESGISPDKILELVTVNAARSLHQENALGRIRPGFRADLIAIPYGGHADL
                     FEQIVAFAGSIDWMTVNGRMKMTKLQ"
     gene            complement(2474..3136)
                     /locus_tag="VE486_01750"
     CDS             complement(2474..3136)
                     /locus_tag="VE486_01750"
                     /inference="COORDINATES: protein motif:HMM:NF036541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HZA37842.1"
                     /translation="MPGFLERALKGAANTSFSFGQILIQEANGGFVVFHREDETRKDL
                     QTCRHAEDAVEIAKYDDAGNYRPLKTAPNLRRGWRLTLKTLEELERALDYFYPGRLAV
                     FAAWKTSQLRTTSLRETLERQSGMYRVAGKISDHQIDGLVGDFCRSDGGCLRTILWKR
                     DRDGAIASTKLPKEKFDPVHDQAATGPRSATAATVPLLCQEPCNLLVAECRKVVTGAR
                     PR"
     gene            complement(3167..3625)
                     /locus_tag="VE486_01755"
     CDS             complement(3167..3625)
                     /locus_tag="VE486_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZA37843.1"
                     /translation="MKQATCKKLQEIGAFALAGGCTRKRMRQLVEMIRAARDYRWIAV
                     YKIVKDEFVILAGTGDDPPAYPRFPVTQGLCGAALDSGKSIIVGDVHNDPRYLPTFHT
                     TRSEIVVPMRNEHRHILGMLDAESDKLNAFGEEDRQFLERAGGLIAHCLH"
     gene            complement(3728..4633)
                     /locus_tag="VE486_01760"
     CDS             complement(3728..4633)
                     /locus_tag="VE486_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HZA37844.1"
                     /translation="MISKGQSGLLIVAHGSTVNPDSSAPTLAHAAEIRRRNLFSDVEC
                     AFWKEEPSLRDALFLFDPKVIREVCVVPNFISEGYFTQTVIPRELELNGSITKRSSGQ
                     VWKYCEPVGNHPLMTELLLRRAKEIAPDIDLAETSLLIVAHGTDLNENSAVAAKREAE
                     KIGALGKYAAVLNVYMEEPPLVSDWRKLTKTSNVIVVPFFISDGLHSYEDIPRLLGIV
                     NSNPESFDFAQDRLRKAESKDLAAKLSDNFTVSLDSTRDGAKMTMRPVFRRNPYKIGG
                     RSLFYAPSVGTDAGIADIIIEQAGN"
     gene            4780..5820
                     /locus_tag="VE486_01765"
     CDS             4780..5820
                     /locus_tag="VE486_01765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZA37845.1"
                     /translation="MRFRTYKNTDLTVSEVGFGLWTISTGWWGNFTEGEAIALMRNAF
                     DLGITLFDAADTYGNGLSEELIAKAFPEQRDEIVIATKVGYDFVHYGEARGRGQREIP
                     QDFSPDAIARATDAALKRLRTDRIDLLQLHNIRMEQVYDDAVWTALEQLKTSGKIRYY
                     GIALGPAIGWLYEGINCIRERDVTSVQHIYNMLEQHPGRAFHDAASEAAKDTMFLIRV
                     THSSGMLEGKYTEETTFPPTDHRSHRPRSWLLNGIKKVDKLRFLENSARTLGQAALQW
                     LLTDNRVASTLPNIYNEEQLIEFANAPECPPLGSDDIATIDHLYSENFGIEEEPPKFK
                     GTMELPKEPAAA"
     gene            complement(5941..7824)
                     /gene="mrdA"
                     /locus_tag="VE486_01770"
     CDS             complement(5941..7824)
                     /gene="mrdA"
                     /locus_tag="VE486_01770"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZA37846.1"
                     /translation="MVATQAGLPAQTFAPPSASSPVPALSASPSPPEETIIPTFETQK
                     LARTYILDVPGPRGQITDRNGVPLAQNRLSYNLAINFPTPLDFSDAQTLSFVRGKIDK
                     TAKLISRTLKIPDQAILRHYRNRGIMPLEIAQNLSPADYNELKDNLPSGVIVRPVYVR
                     VYPKGKVAGQIVGYTGKTGRNPDGIVDNHETLWPETEGREGLEQTFNQMLTGKHGEYK
                     LTFDKDGRKTSERLVTPPEPGYNVVTTLDVRLQELAEKALEAKAKRGAIVILDPNNGD
                     VLALASWPTYDPNLFVPSISAEQLKALQDDPNIPLLPRAYRSSYPPGSTFKIAVGIAA
                     IESGAVRSDDQFNCVPAIQIGNVTFHNWKKGDRGALNFVQALTESCDTWFYQVGIKTG
                     AQPIIDWALKLGFGAKCGIPLRGEAEGRVPNDEYMKATHGRKLLNGDIANLSIGQGDL
                     QVTPLQMAQSMGIVANGGTFYQTRLVQQVQTFNNEIVTAYQVRAKRTLDLLSETRDQL
                     RIGMVDAVNGRGGTGHQASLENVEVAGKTGTAQWGPKHKERTAAWFAGFLPADQPQYA
                     FAALYEGDVGSNVHGGSSAAPMIADVFKEIYKGQQMAGRQRREQEPEIRRAEPVEEED
                     ESD"
     gene            complement(8037..9674)
                     /locus_tag="VE486_01775"
     CDS             complement(8037..9674)
                     /locus_tag="VE486_01775"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZA37847.1"
                     /translation="MPLRKEKPDLQTKVHNVPHKPGVYLMRDRFNRVIYVGKARDLRK
                     RVSSYFLPSKIAQADLKTRAMLDAVWDFETHIVRSDAESVLLEGKLIKEYRPRYNVSF
                     RDDKRFLLVKVDMSEEWPRFRLARFKKDDGARYFGPYAHSGALRQTLNFMRKKFGVLT
                     FGRGSPTERELKSATYQVPMHLSEINAGQYRERVVQASEFLEGHSREMIATLEEEMRK
                     TAEKMDFEKAAELRDMIDDLRSTTKPMRRFTRGSLPSAIDPMADVQALADALQLPLAP
                     HVMECFDISNISTTHVVASMVCFRNGVSDKDNYRRYRVRTVEGQDDFASMAEVVRRRY
                     SRVLLEAREANPDAVEFSQENPTETMARFASYPERSRGTPPQNAEVTPRDSSTPLRPA
                     RNDESFVAVRLPDLIIVDGGKGQLSAACRELQRLGLHDLPIIGLAKEREEIYRPGRAL
                     PLQLPMDSGALRLLQRIRDEAHRFANAYHQLLMKKRIGESILDDCPGVSQNRKNLLLR
                     KFGSVKRLRKATVEEIASTEGIGRKLAEEVHRFLQRH"
     gene            complement(9788..10930)
                     /locus_tag="VE486_01780"
     CDS             complement(9788..10930)
                     /locus_tag="VE486_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZA37848.1"
                     /translation="MSMSPIQDWRAIQYRSRLIRMGWSLPIFRIAGIQLRIHVTFLLL
                     IAWLAFGYYAEGGSAVAASRVIFILLLFVCVVLHEFGHAFAAKAFGINTPDITLLPIG
                     GVARLERMPEEPVQELIIAVAGPMVNVVIALGLFVAGGSQALVNPSAVEGGGFIAQLM
                     TINILLVLFNLLPAFPMDGGRVLRALLAIRMSYARATQVAATIGQGFAFVFGFIGLLW
                     NPFLIFIALFVYIGASQEAALAQMKDVSRRFPVSSAMVREFRTLPENATLQEAVDALL
                     ATSQHDFPVVNETGNVAGVLTRHDLIAALRKNDPAIRVGDVMRRDIPTVTTGTRFEEA
                     FRIMQESNCPAVPVLDSMKRLVGLLTPENVTELMMIQSAMPRRRVT"
     gene            10975..12123
                     /locus_tag="VE486_01785"
     CDS             10975..12123
                     /locus_tag="VE486_01785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZA37849.1"
                     /translation="MLITDHRSLVDWTMETFDVAIVGAGPAGSSCAAFCALGGLRALV
                     LEREKFPREKVCGDCLNPSSWTILRRLGIADHVRQLPHAVLNTVEFIAISGQKVIVDI
                     ASGQESLIAIKRSLLDALVMSRARELGAQIHEETTVMELTQTTKGSDWKIKTSYGRTL
                     AARVLVGADGRNSTVARLCNLLPRPARERVALQAHIPLPAGFGNRVVLQFLPEGYSGQ
                     APVNDGELNLCLVSKPSNISALRRWAEQRFTLQRDHSWHTITPLTRVALSPAHDKLFM
                     VGDAARVVEPFTGEGIYYALRSGELAAQAIVTAVRANYDGDPVNDYVQAHTAMYRGRL
                     WVNRLARAAVLSPRVASFLLRFAPLSPAIMRLLTAKIALSTMTMSNVE"
     gene            complement(12202..>12741)
                     /locus_tag="VE486_01790"
     CDS             complement(12202..>12741)
                     /locus_tag="VE486_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HZA37850.1"
                     /translation="VDGFHPENVAKLVIGDPSGFVPCTPLGVQRLLLESKIDIDGAHV
                     VIVGRSMIVGKPLALLLMQKNKNANATVTVVHSRSRDLEKITRLADILIAAIGQANFV
                     KAAHVRDGATVIDVGINRVEDAASERGYRLTGDVAFDEVSKKASAITPVPGGVGPMTI
                     AMLMWNTVKAARQSLSRLQ"
BASE COUNT         2697 a         3667 c         3536 g         2841 t
ORIGIN      
        1 ggggcgtttg gaggatgagc aggctttgtt gaagctgcgg cgcaagaatt tgttggaggg
       61 taaggctctg cgactggtgc attccttgtc cggccatacg ccgagtttac cggtgcagcc
      121 ggcgacaagc aagcaattca gagcgcgaga aacgctgtag ccgcctcgct atgtcgaggc
      181 gctcatcgcg ttatcaccgg cacggcgaca cagcgccgtg gctacaagcg ccgccgcgca
      241 tgaatgccat ggctacagga atgcagtatc cagcccagca tccagaatcc ctcacactcc
      301 cgccatgtcc catttcctga tcgtttcgtg aatcagaccc gcgatcttgt cgttcacctt
      361 cgtgaaatag atcttcgctt ttaccagatc gaatttcggg taaccctggc cttccttgta
      421 gagcatgatt gtcgacggga agggatatgc gctccacgtg ttcggctggg gaagcgcagt
      481 caccatctca ggtttgtaat gctttttctg aatgagagcg ggatcgatcg ccatgatgta
      541 tgcggtctca ttttctgctc cgtgaccgcc gtgttcgcca aacacctcca gcgtcacatc
      601 gctgcaatag gaccaccagt tcaaaaccag aattctcgtg accgtttcgc gtcccacttc
      661 ctgtgcgagc gcactcagga ccgcagtctg gccgccgcca tggccgttga gcaggataat
      721 gtttttgaat tggttcttcg ccagaccggc gagaacggcg tgcacgtaat cgcgaaaagc
      781 tgcctctgga atcgtgaagc tacccggata tgcatccagg actccagtga atccgtaggg
      841 aatcgctggc gcgaccaatg cattgacgcg cggagcgatc tcgtgcgcca ttgccaccgg
      901 cgcaagaatg tctgtgccgt tgggtgcgac gccgtgcggc tcgagtgtcc ccagcggcag
      961 caagaccgtc cggattttcg ccgggaccca ttcgcgaaat tcgatccagt tgatgcgctc
     1021 catctcgcgc gtgctcggcg aacttttctt agcggtactc attggggcag attagcagca
     1081 gcgcgcgcat cctgctgtcg ttttcctaaa tccggtctcc ttccttcgtc atttgtcatt
     1141 ggagcttcgt cattttcatt cttccattca ctgtcatcca atcgattgac ccagcgaatg
     1201 ccacgatctg ctcgaaaaga tccgcatgtc caccatacgg aatagctatg agatcggctc
     1261 gaaatccggg ccgaatccgt ccaagggcgt tctcttgatg caacgatcga gccgcattca
     1321 ccgtcaccag ctcaagaatt ttatctggtg aaatgccgga ctcgctgcgc tgaaacgcgc
     1381 gcatttcggc gaacaaactc aggtcctcgt tgctggcaag actatcggtg cccaggcaaa
     1441 tgttaaaccc aagcccgcgc aatttttcga agcgaaagcg gctatgccca aaataatcgt
     1501 gacttcgcgg gcaatgcacc agatgaaact tgtttgttga ttctttgagc aatttgaagc
     1561 cgctttcggc gagttcgttt aaatgagcga cgatccattc atttggtagg gcgcgaccgg
     1621 agcctgccct gagcgaagtc gaagggggcg cgccggcaat attggcaaaa aatgccaacg
     1681 gcgtctcttg cccgcagtca tccatcggtc gaccgatgct tttcataaat tcatagagcg
     1741 gccccgactg attgcgaaac atctccagct cctcgcgcga ttcggccaga tgcgttgtta
     1801 acaaaatgct ctggcgtcgg gccacctcct cgcaaagccg atataaattt gatgaagcag
     1861 tgaacggtgc atgcggtgcc agtccccggt tctccgccgg ctctagcgat tcaattgcgc
     1921 gatcgacaat ttcattcgca cgttccggcg aacgcacgtc gatcaactct gcaaaccacc
     1981 atgtccgaat cggcgtctgg atctcggaaa tcagctcggg aaatgctgtg aggttcgcga
     2041 ccgtcgtcgt tccgaatctt tttgcttccg cgaacccttc cgcgatcgac acgagataat
     2101 cctgccgcga aagcttcgac ttctccgtgt tgatcgcgcg aatccaatcg gtaaaggatt
     2161 tctgcggccg gattttgccg cgcaagcacg tgtaatccag atggcaatgc gcgttgatga
     2221 gtccaggaag cagtgcccgc tcgccaagat cgacaacttc caggcctgaa tgccgagcgg
     2281 aaatctcagg aaactttcca acatcgataa tttgattttt ggagatcgcg atcgcgccgt
     2341 tctcaattgg cggcccatcc atcgtgacca cgatgcgcgc gcggatgatc atttttctgc
     2401 tgtagcggcg gtctatgacc gtcgcaaata tcgggacaaa ataatgcgac gctcatagag
     2461 cgccgctaca gattcagcgc ggtctcgcgc ctgtcacaac ttttcggcat tctgccacga
     2521 gcagattgca cggttcctgg caaagcaggg gtaccgtagc cgcggtcgcc gaccgcggtc
     2581 cggtcgcagc ttggtcgtga acgggatcaa acttctcttt cggcaacttt gtcgatgcga
     2641 tcgcaccgtc ccggtcgcgt ttccacaaga tggttcgcag acacccgccg tcggaccggc
     2701 aaaaatcgcc cacgagacca tcgatttgat ggtccgaaat tttccctgca acacggtaca
     2761 tgcctgattg tcgctccaat gtttcacgca gactggtcgt gcgcagttgg ctagttttcc
     2821 aagcggcgaa gacggccagc ctcccgggat aaaaataatc gagcgcgcgc tctaattcct
     2881 cgagcgtttt gagtgtcaat cgccagccgc gccgcaagtt tggcgcggtt ttgagcggac
     2941 gataatttcc ggcatcgtca tacttcgcga tctctactgc atcttctgcg tgacgacacg
     3001 tttgtagatc tttacgagtt tcatcctccc gatggaacac gacaaaaccg ccgtttgcct
     3061 cctggatcaa gatttggccg aaagagaacg aagtattcgc tgcgcccttg agagctcgct
     3121 cgagaaaacc tggcattggt agggcgcgac cgccgggcgc gccgtgctaa tgcaggcaat
     3181 gggcaatcaa accacctgcg cgctccagaa attgtcgatc ttcctcgcca aaggcgttca
     3241 atttatcgct ctcggcatcc agcatgccga gaatgtgccg gtgttcattt cgcattggga
     3301 caacaatctc tgagcgcgta gtatgaaatg tgggcagata gcgcgggtcg ttgtgcacgt
     3361 cgccgacaat gatcgatttg cccgaatcga gcgctgcccc gcacagcccc tgcgtaacgg
     3421 gaaagcgcgg atacgccggt gggtcgtcgc cagtgccggc gagaattacg aattcatctt
     3481 tgacgatttt gtaaaccgcg atccaccgat aatcgcgcgc cgcgcgaatc atctccacga
     3541 gttgtctcat tcgcttgcgg gtgcatccgc cagccagcgc gaatgcgccg atctcctgaa
     3601 gcttcttgca cgtcgcttgt ttcattcgcg gggagagcaa tctcgcgcaa aagaaaacaa
     3661 gcgcgagcga gatttcaact ttttcgcgat ctaattttcc tcaaccgagg aaagacggca
     3721 ccagcggcta atttcctgcc tgttcgatga tgatatcggc gatgcccgca tcggtgccga
     3781 cggacggcgc atagaataag gaacgaccac cgatcttgta gggattgcgg cgaaaaacag
     3841 ggcgcatcgt cattttcgcg ccatcccgag tggagtcgag ggagaccgtg aagttatcgg
     3901 agagtttcgc tgcgagatct ttcgactccg ctttgcggag cctgtcctga gcgaagtcga
     3961 aggactcagg attactgttg acgatgccaa gcagcctcgg aatgtcttcg tagctgtgca
     4021 agccatctga aatgaaaaac ggtaccacga tcacgttcga agtcttcgtc aatttccgcc
     4081 aatcggaaac cagcggcggt tcttccatgt aaacgttgag gacggccgca tattttccga
     4141 gtgcaccgat tttttccgct tcgcgcttcg ccgcaactgc gctgttttca ttcaaatccg
     4201 tcccgtgtgc gacaatgagc aagctggtct cagcgagatc gatatcaggt gcgatttctt
     4261 tcgcccgtcg caaaagcagc tccgtcatca gaggatggtt tcccactggc tcgcaatatt
     4321 tccagacttg gccgctcgat cgttttgtga tgctcccatt cagctcaagt tcgcgcggga
     4381 tcaccgtctg cgtgaaataa ccttcgctga taaaattcgg cacgacgcaa acttcgcgaa
     4441 tcacttttgg atcgaacaaa aacaacgcgt cgcgcaagct cggctcttct ttccaaaaag
     4501 cgcactccac atcgctaaaa agatttcggc gccgaatctc cgccgcgtgc gccagcgtcg
     4561 gcgcgctcga atccgggttc accgtcgagc cgtgggcaac gatgagaagc ccgctttgac
     4621 ctttcgaaat catgttacct cggaaaaacg tcgaacatcg aacatccaac gtccaatgcc
     4681 gaatttaagg catgccggcg tggtgttttg ctgacttcaa tgttcggcgt tcgatgttgg
     4741 atgttggacg ttttattctt cattcgctaa actgaagcca tgcgtttccg cacgtacaag
     4801 aacaccgacc tgacggtgag cgaagttggc tttgggctct ggacgatttc taccggttgg
     4861 tgggggaact ttaccgaggg cgaagccatc gcgctcatgc gcaacgcgtt cgatctcggg
     4921 atcacgttgt tcgatgcggc cgacacctac gggaatggat tgagcgagga attgatcgcg
     4981 aaggcttttc cggaacagcg ggacgagatc gtgatcgcga ccaaggtcgg ctacgatttc
     5041 gtccattacg gcgaggcgcg tgggcgcggt cagcgcgaaa ttccgcagga cttttcgccg
     5101 gatgcgatcg cgcgcgcaac ggatgccgcg ctgaagcgat taagaacaga ccggatcgat
     5161 ctgcttcaac tgcacaatat ccggatggag caggtatatg atgacgcggt ctggacggca
     5221 ctcgagcaat taaaaacctc tggcaaaatc cgctattatg gcatcgcgct gggaccggcg
     5281 attggctggc tttacgaggg aataaactgc attcgggagc gcgacgtgac gagcgtgcaa
     5341 cacatttaca acatgctcga acaacatccc ggacgcgcct ttcacgatgc cgcaagcgag
     5401 gcggcgaagg acacgatgtt tctcattcgc gtgacgcact cgtctggaat gctggaggga
     5461 aaatataccg aggagacgac gttcccgccg accgatcatc gcagccatcg gccgcgcagt
     5521 tggctgctca acgggatcaa aaaagtcgac aagttgcgct ttctcgaaaa ctccgcccgc
     5581 acgctggggc aggcagcttt gcaatggttg cttaccgaca atcgcgtggc ctcgacgctg
     5641 cccaacattt acaacgaaga acagctcatt gaattcgcca atgcgcccga atgcccgccg
     5701 ctcggcagcg atgacatcgc cacgatcgac catctctatt ccgaaaattt tggaattgaa
     5761 gaagaaccgc cgaaatttaa aggaacgatg gagttgccaa aggaaccggc ggccgcgtag
     5821 acacgaattc agacgaaggg cattgtagcg gcgctctgtg agcgccgaaa ccttcgcacg
     5881 caagatccga cggcggtcat agacccgccg ctacagcgaa aattcgtgta attcgtgtat
     5941 ctaatcggac tcgtcttctt cctcgaccgg ctcagcgcgg cggatttctg gttcttgttc
     6001 gcgtcgctgg cgtccagcca tctgttgtcc cttgtaaatt tctttgaaga catcggcgat
     6061 catcggcgct gcgctagagc cgccgtgcac gttgctgccg acatcgcctt cgtagagggc
     6121 ggcgaacgca tattgtggct gatcggccgg caggaatccc gcgaaccagg ccgcggtgcg
     6181 ttctttgtgt tttggtcccc attgcgcagt gccggttttt cccgcgactt ccacattctc
     6241 gagacttgcc tgatgtccgg tgccgccccg gccattcacc gcgtcgacca tcccgatgcg
     6301 caattgatcc cgcgtctcgg ataacaaatc gagtgttctc ttggcgcgca cctgatacgc
     6361 agtcacgatt tcgttattga atgtctgcac ctgttggacg agccgggttt gataaaatgt
     6421 gccgccattg gcgacaatcc ccatcgattg ggccatttgc agcggagtga cttgcaggtc
     6481 gccttggccg atcgacaggt tcgcgatgtc gccgttgagc aatttgcggc cgtgggttgc
     6541 cttcatgtat tcgtcattcg gcacgcggcc ttccgcctca ccgcgcaacg gaatgccgca
     6601 tttggcgcca aagcccagct ttaacgccca atcaatgatc ggctgggcgc cagtcttgat
     6661 tccaacctga taaaaccagg tgtcgcacga ttcggtcagc gcctggacga aattcaacgc
     6721 gccgcgatcg ccctttttcc aattgtgaaa cgtgacgttg ccaatttgaa ttgccgggac
     6781 gcagttaaat tgatcatccg agcggactgc gccgctttcg atcgcggcga tgccaaccgc
     6841 gatcttgaat gtcgatcctg gcggataaga cgagcgatat gcgcgcggca acagcggaat
     6901 gttgggatca tcctgcaagg ctttgagttg ctcggctgag atcgagggaa caaacagatt
     6961 cgggtcgtag gtcggccatg aagcaagcgc aagaacatcg ccattgtttg gatcgagaat
     7021 cacgatcgcg ccgcgttttg ctttcgcttc cagcgccttt tccgccagtt cctggagacg
     7081 aacatccaga gtggtaacga cattgtaacc aggctcaggc ggggtgacca gccgttcgga
     7141 ggttttccgt ccatccttgt cgaaggtgag tttgtattcg ccatgttttc ccgtgagcat
     7201 ttggttgaac gtttgctcga gcccctcgcg cccctcggtt tcgggccaga gtgtttcgtg
     7261 gttatccacg atcccatcgg gatttcgacc ggtttttccg gtgtagccga cgatttggcc
     7321 ggcaactttc cctttcgggt aaacgcgcac ataaacgggc cgcacgatta ctcccgacgg
     7381 caagttgtct ttgagttcat tgtaatccgc cgggctgaga ttctgcgcga tctccagcgg
     7441 catgattccc cggttgcgat agtggcgaag gatcgcttga tcgggaatct tgagtgtgcg
     7501 gctgattagt ttcgcagttt tgtcgatctt tccccggacg aagctgagcg tctgggcgtc
     7561 ggaaaaatca agcggcgtcg gaaaattgat cgcgagattg taactgagtc gattctgcgc
     7621 gaggggcaca ccattgcggt ctgtgatctg gccgcgggga cctggaacat cgaggatgta
     7681 ggtgcgcgca agtttttgcg tctcaaaagt tgggatgatt gtttcttcgg gcggacttgg
     7741 actggccgaa agtgccggga ccggactgga cgcagatggt ggcgcgaacg tctgggctgg
     7801 caagccagct tgtgtcgcca ccatcaccaa tagaaaggca gggatggctc tcgctccagc
     7861 atgagtggca caggcgtcgc gtctgtgagg cgagcaggcg tccccgcctg cgagtcttga
     7921 cggaacggca ggcgagaggc ccgcgggacc cacagccagg atggccgtgc cacgcgcgcc
     7981 ggagcaaccc ttccctgctt ggcaacccgg aagcatccgc ccgaatctgc gcagtctcaa
     8041 tgccgctgca agaagcgatg cacttcttcc gcaagctttc ggccaatgcc ttccgtggag
     8101 gcaatttcct ctaccgtcgc tttgcgcaga cgtttcacgg agccgaactt tcgcagcagc
     8161 aaatttttcc ggttttggct cacccccggg caatcatcga gaatgctctc gccgatgcgc
     8221 tttttcatga gcagttgatg atacgcgttg gcaaaccgat gggcttcgtc acggattcgc
     8281 tggagcaagc gcaaagcgcc ggaatccatt ggaagttgca gcggcagcgc ccggcctggc
     8341 cgataaatct cctcccgttc cttggccagg ccgatgatcg gtagatcatg caaacccaat
     8401 cgctgcagct cgcggcacgc ggcggaaagc tggccttttc cgccatcgac aatgatcaag
     8461 tccggaaggc gaaccgcgac aaaagactcg tcattccgag cggggcgtag cggagtcgag
     8521 gaatcccgcg gagttacctc tgcgttttgc ggcggggtcc ctcgacttcg ctcgggataa
     8581 ctggcgaatc gcgccatcgt ctctgttgga ttctcttgcg aaaactctac cgcgtccgga
     8641 ttcgcttcac gcgcttccag cagcacccgc gaataacgcc gccgtaccac ctcggccatg
     8701 ctggcaaaat catcctgtcc ttccaccgtg cgcactcgat aacgccggta attgtccttg
     8761 tccgacacgc cgtttcggaa acaaaccatc gaggcaacta catgcgtcgt agaaatattt
     8821 gagatatcga agcactccat gacgtgcggc gccaggggaa gttgcaacgc atccgccagc
     8881 gcctgaacat ccgccattgg atcgatcgcg ctcggcagac tgccgcgggt gaaacgccgc
     8941 atcggctttg tcgtactgcg caaatcgtcg atcatgtccc gaagctcagc cgccttctcg
     9001 aagtccatct tctccgcggt cttgcgcatt tcctcttcca acgtggcgat catctcccgc
     9061 gaatgtccct cgaggaactc actcgcttga acgacgcgct cgcgatattg cccggcgttg
     9121 atttcgctca agtgcatcgg cacctgatag gtggccgatt tcaattcgcg ctcggtcggc
     9181 gagccccggc caaacgtgag cacaccgaat tttttgcgca tgaaattaag cgtctgtcgc
     9241 agcgcgcccg aatgcgcata cggcccgaag tagcgggcgc cgtcgtcctt tttgaatcga
     9301 gccagccgga aacgcggcca ttcctccgac atgtcaactt tgacaaggag aaaccgttta
     9361 tcgtcgcgga aggaaacgtt gtaacgcggc cggtattcct tgatcaattt gccttccagc
     9421 aaaacggatt ccgcgtcgct gcggacgatg tgcgtttcga aatcccaaac ggcatcgagc
     9481 atggcgcgcg tcttcaaatc ggcttgcgcg atcttcgagg gcagaaagta tgagctgaca
     9541 cgcttgcgca ggtctcgcgc tttaccgacg taaatcacgc gattaaagcg gtcccgcatc
     9601 aggtacacac ccggtttgtg cggcacattg tgcacctttg tttgcagatc cggtttttct
     9661 ttccttagcg gcatcgcctt ttcggaagat ggacaatcgc ttcgccagaa ttgtagggca
     9721 agcgcgccgc ttgccgattt gggccgttgg caggcggagc acccgcccta cagacgtgca
     9781 aattgaccta cgtaacccgc cggcgcggca ttgccgactg aatcatcatc aattcggtga
     9841 cgttttcggg agtaagcaaa ccgaccaggc gtttcatgct gtcaagcact ggcacagccg
     9901 ggcagttgct ttcctgcata atgcgaaacg cttcttcgaa acgcgttccc gtcgtgacgg
     9961 tgggaatatc gcgccgcatt acgtcgccga ctcggatcgc gggatcattc ttccgcagcg
    10021 ccgcgatcag atcgtgacgc gtgagcacac cagcgacgtt tcccgtttca ttcaccaccg
    10081 gaaaatcgtg ctgtgaggtc gcgagcaatg catccacggc ttcctgcagg gtcgcatttt
    10141 ctggaagggt gcggaactcg cgcaccatcg cgctggaaac gggaaatctc cgggagacat
    10201 ctttcatctg cgccagggcc gcctcctgcg acgcgccgat gtaaacgaaa agggcgatga
    10261 agatcaggaa cgggttccag aggagcccaa tgaagccaaa gacgaacgca aacccctggc
    10321 cgatggttgc cgcaacttga gtcgcgcgcg cgtaactcat ccggatcgcc agcagcgcgc
    10381 gtagcacccg accgccatcc attggaaatg cgggcagcaa attaaaaagg accagcagaa
    10441 tgttaatggt catcaactgc gcaatgaacc cgccgccttc cactgctgat ggattaacaa
    10501 gcgcctgtga cccgcccgct acgaacaaac caagcgcgat cactacattt accattggtc
    10561 cggccacggc gatgatcaat tcctgcaccg gttcctccgg catccgttcc agtcgcgcga
    10621 ctccaccgat ggggagcaac gtgatgtcgg gcgtgttaat cccgaaggcc ttcgccgcga
    10681 atgcgtgccc gaattcgtga agcaccacac agacgaatag gagcagaata aatattacgc
    10741 ggctcgccgc caccgccgaa ccgccttccg cataataacc gaaggcaagc cacgcgatca
    10801 gcagcaaaaa cgtcacgtga atccgcaatt gaatgccggc gatgcggaag attgggagag
    10861 accagcccat gcgaatcaat ctgctccggt attgaatcgc gcgccagtcc tgaataggcg
    10921 acatggacat cggggcgatg catgtgatca gtggtccgtg atcggtgaac ggacatgctg
    10981 atcactgatc accgttcact ggttgattgg acaatggaaa ccttcgatgt cgcaattgtc
    11041 ggcgccggtc cggcaggatc gagttgtgcg gcgttttgcg cgcttggcgg attgcgcgcg
    11101 ctcgttctcg aacgcgagaa atttccccgc gaaaaagtct gcggcgattg tctgaatcca
    11161 tcctcctgga caatcctgcg acgactggga attgcggacc atgtccggca actgccgcac
    11221 gccgttctta ataccgtgga gttcatcgcg atcagcggac agaaagtcat tgtcgatatc
    11281 gcctccggcc aggaaagctt gatcgcgatc aagcgcagct tgttggacgc gctggtgatg
    11341 tcgcgagcgc gggagctggg tgcgcagatt catgaagaga cgaccgtcat ggagttgacc
    11401 caaacgacga aaggttcgga ttggaaaatt aagaccagct acgggcgaac ccttgccgcg
    11461 cgagtcctag tcggcgcgga cggtcgtaac tcgacagtgg cgcgcctttg caatttactc
    11521 ccacgacctg cccgtgaacg cgtcgcgcta caagcgcaca ttccgctgcc ggcgggtttc
    11581 gggaaccgcg tcgtgctcca atttctgcct gaaggatatt cgggccaggc gccggtcaat
    11641 gacggtgaat tgaatctgtg tctcgtgagc aaaccttcca acatttctgc gctccgccgc
    11701 tgggcggagc agcgctttac ccttcaacga gatcattcgt ggcatacgat cactccgctc
    11761 acccgggtcg cgctttctcc ggcgcacgac aagctgttta tggtcggtga cgcagcgcgg
    11821 gtggttgagc cgtttacggg tgaggggatc tattatgcgc ttcgctcagg cgagctggcg
    11881 gcgcaggcga tcgtgacggc tgtccgcgca aattatgatg gcgatccagt gaatgattac
    11941 gtgcaggcgc acacagccat gtatcgcggc agattatggg ttaaccgcct ggcgcgcgcg
    12001 gcagtcttgt cgccgcgcgt cgcgtctttt cttctgcggt ttgcgccgtt aagtccagcg
    12061 atcatgcgat tgctcacggc aaagatcgcc ttgtccacaa tgacaatgtc gaatgtcgaa
    12121 tgacgaagga atgacgaaaa ccgaagcagg gaatcgtgtt cggactttgt cattgtctcg
    12181 ggattcgtca ttcgtgcttc gtcattgcaa tcgcgaaagc gattgccggg ctgctttcac
    12241 cgtgttccac atcagcattg cgatcgtcat cgggcccaca ccaccgggca ccggcgtgat
    12301 cgcgctcgcc ttttttgata cttcgtcaaa ggccacatcc cctgtgagtc gataaccacg
    12361 ttcgctcgcc gcgtcttcta cacgattgat accgacatcg ataacggtcg cgccatcgcg
    12421 cacgtgtgcc gctttcacaa agttcgcttg accaatcgcc gcgatcaaaa tgtccgctaa
    12481 ccgggtaatc ttttcgagat cgcggctgcg cgaatgcacg accgtcactg tcgcattcgc
    12541 gtttttgttt ttctgcatca gaagcagggc cagcggtttg cccacgatca tactgcgtcc
    12601 aacaatcacg acgtgcgcgc cgtcgatgtc gatcttgctt tcaagcagca ggcgctggac
    12661 cccgagtggg gtgcatggaa caaatccgct gggatcgccg attacgagct tcgccacatt
    12721 ctccgggtgg aaaccatcga c
//
LOCUS       DASQUQ010000064         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_468490, whole genome shotgun sequence.
ACCESSION   DASQUQ010000064 DASQUQ010000000
VERSION     DASQUQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468490"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..710
                     /locus_tag="VE486_01795"
     CDS             120..710
                     /locus_tag="VE486_01795"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZA37851.1"
                     /translation="MNISAVLLAGGQSRRMGRDKATILFRGKPLWQIQLGLLRKLQPA
                     EIFVSARTDPAWRPPDVTFVPDEPPSRGPLSGLTATLARIQSSHLLALAIDMPLMNEE
                     HLRFLCSEIQRGCGVLPMIGDLAEPLAAIYPAEACDTFSAALSGNDFSLQALTNQLVK
                     TGKLRAVRVPEEEQRCYWNFNEPADMYDQPGSRIAF"
     gene            765..1073
                     /locus_tag="VE486_01800"
     CDS             765..1073
                     /locus_tag="VE486_01800"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HZA37852.1"
                     /translation="MNRLRNLWNLENIKIVRRIIVSVVGATVLLIGIALLVLPGPAFI
                     VIPIGLAILATEYAWARRWLNRARQIASGVVSGRKDATQDRQMQDAVPNGATDRVERD
                     "
     gene            1120..1953
                     /locus_tag="VE486_01805"
     CDS             1120..1953
                     /locus_tag="VE486_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZA37853.1"
                     /translation="MATEPLMEKKPDSAASVLLTPPERADEIFLEGPRSRFAEFFTLL
                     RVMRDFLRGFRTLHFVGPCVTVFGSSRIKRDDPHYELARKMGAAIARLGFTVMTGGGP
                     GIMEAANRGAKEVAGRSVGCTIELPSEQPANAYLDRCVRMHYFFVRKALLVKYSYAFV
                     VMPGGAGTLDELFEAVTLIQTGKIKNFPIVIMGTDYWKELIGFIDKMAHHGMIAAVDL
                     KLIYATDSVEEAIAHIRRKAIEPFGLKSRRRKTRRSFPWLGERGLQTPVESAPRSDMQ
                     P"
     gene            2000..>2692
                     /locus_tag="VE486_01810"
     CDS             2000..>2692
                     /locus_tag="VE486_01810"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZA37854.1"
                     /translation="MAKTAPAALTCQICKGPKSPHDGMMAELIRPSLAEFIKKRVPEL
                     DDKGFVCLDDLGEFRKDYVKEVLEDEIGELSVLDQEVVESLQEHEILSSDIEKQFERK
                     LTFGERLSDRIAEFGGSWKFLISFGGVIFVWVAANGVLLVTRAFDPYPFILLNLILSC
                     LAAVQAPVIMMSQNRAEARDRLRAENDYKVNLKAELEIRHLHEKIDHLLRKQYNRLFE
                     IQQIQIELLEEIG"
BASE COUNT          594 a          740 c          749 g          609 t
ORIGIN      
        1 atgaatcagg ctattgcccc gggcgcggaa gaatttctca aaggacgcgg ggggttgcgc
       61 gccaaaattc tcattgatgg cgagctgcgc tcgacggcac tcgtccccgc cgcaatggga
      121 tgaacatcag cgcggtgttg ctcgccggcg gtcaatcgcg tcgaatgggc cgcgataaag
      181 ccaccatcct ttttcgcgga aaaccgcttt ggcaaattca actcgggctc ctgcgcaaat
      241 tgcagccggc agaaatcttc gtttcagcgc gaactgatcc ggcctggcga ccgccggatg
      301 tgacgtttgt gccggacgag cctccatcgc gcgggccgct tagcgggttg accgcgacat
      361 tggctcgaat ccaaagcagt catctgctcg ctctcgcgat cgatatgcca ttgatgaacg
      421 aagaacactt gcgttttctt tgcagcgaga tccagcgagg ttgcggcgtc ttgccaatga
      481 tcggcgatct ggctgaaccg ctggccgcaa tttatccagc tgaggcctgc gacacatttt
      541 ccgcggccct ttcgggaaat gatttttcgt tgcaggcgtt aacgaatcaa ctcgtgaaaa
      601 caggcaagct gcgggccgtt cgagtgcccg aggaagagca acgatgttac tggaacttca
      661 acgaacctgc tgacatgtac gaccagcctg gttcgcgaat tgcgttctaa atcgaattaa
      721 tcgagcgttg ttgcatgtag gcaaataacg cccgcatgaa ttggatgaat cgtcttcgca
      781 acctttggaa tcttgagaat atcaagatcg tcaggcggat tatcgtctcc gttgttggag
      841 ccacggtgtt actgatcggc attgcgctcc tggttttgcc gggcccggcg ttcatcgtta
      901 taccgattgg actcgctatt cttgctacgg aatacgcatg ggcgcgccgt tggttaaaca
      961 gagcccgtca gatcgcgagc ggcgtcgttt ccggccgcaa agacgcaacc caggatcgcc
     1021 aaatgcagga tgccgttccc aacggggcga cggatcgcgt cgagcgggat tgagattgtg
     1081 cggcaggtcg ccaatagcaa tcatcgccgt tgaatcggca tggccaccga acctctgatg
     1141 gaaaaaaagc cggattcagc ggcttccgtt ctcctcacac cgccggagcg cgcggacgag
     1201 atttttctcg aagggcctcg ctcgcgtttt gccgagttct ttaccctttt gcgagtgatg
     1261 cgcgatttcc tacgcggatt tcgcaccctg catttcgtcg gtccatgcgt aacggttttt
     1321 ggctcgtccc gcatcaaacg cgacgatccg cattatgaac tcgcccgaaa aatgggcgcg
     1381 gccattgccc ggcttgggtt tactgtcatg accgggggtg gccccgggat tatggaagca
     1441 gcgaaccgcg gcgctaaaga agtcgctggg cgttctgtcg gttgcacgat cgagcttccc
     1501 tctgagcaac cggcaaatgc ttatctcgac cgctgtgtgc gaatgcatta cttcttcgtc
     1561 cgtaaagcgc tcctagtgaa gtattcctat gcttttgtag tcatgcccgg cggcgcaggc
     1621 acgctcgacg agttattcga agcagtcacc ctgattcaga ccgggaaaat caaaaacttt
     1681 ccaatcgtga tcatgggcac cgattattgg aaggaactga tcggtttcat cgacaagatg
     1741 gcgcaccacg gaatgattgc cgccgtggat ctaaaattga tctacgccac cgattcggtg
     1801 gaagaagcca tcgcccacat tcgcaggaaa gcgattgagc cgttcggact caaatcccgc
     1861 aggcgcaaga cgcgacgttc cttcccctgg ctcggcgagc gcggcctcca gacaccggtt
     1921 gaatcagcgc cgcgatcaga tatgcagcct taaattcgac aaatcggctg tagctgcgct
     1981 actctccaga tcacatccga tggccaaaac cgcgcccgcc gcactcacgt gtcagatctg
     2041 caagggacca aagtcgccac atgacgggat gatggccgaa ttgatccggc cgtcgctcgc
     2101 tgaattcatc aagaaacgcg tgcccgagtt ggacgataaa ggttttgtct gcctcgacga
     2161 tctgggcgaa ttccgcaaag attatgtgaa agaagtcttg gaggatgaga tcggcgaact
     2221 ttccgtactc gatcaagaag tcgtcgaaag cttgcaggag cacgagattc tctccagcga
     2281 tatcgagaag caattcgaaa ggaaactcac ctttggggag cgcctttcgg accgcatcgc
     2341 cgagttcggc ggcagttgga agtttctcat tagctttggc ggcgttatct tcgtgtgggt
     2401 tgctgcgaac ggcgtgctgc tcgttaccag agcgttcgat ccgtatccat ttattttgct
     2461 gaacctcatc ctctcttgtc tcgccgccgt ccaggcgccg gtcatcatga tgagtcaaaa
     2521 ccgggccgaa gcgcgcgacc gcttgcgcgc agaaaatgac tataaagtta atctaaaggc
     2581 tgagctggag attcgtcacc tccacgaaaa aatcgaccac ttgttgcgga agcaatacaa
     2641 tcggctgttc gagattcagc agatccagat cgagttgctc gaagaaatcg gc
//
LOCUS       DASQUQ010000065         6949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_734952, whole genome shotgun sequence.
ACCESSION   DASQUQ010000065 DASQUQ010000000
VERSION     DASQUQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734952"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VE486_01815"
     CDS             complement(<1..277)
                     /locus_tag="VE486_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37855.1"
                     /translation="MRLQDADANTLEACTPGATRRLYHRFFKHLRFWPLFRHSTFGFR
                     IFDLMALRDIFKFRELPESETTKPFLEHLEDLRWTVVKMAITLVIAMI"
     gene            complement(322..912)
                     /locus_tag="VE486_01820"
     CDS             complement(322..912)
                     /locus_tag="VE486_01820"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZA37856.1"
                     /translation="MERSNLVRDGKGWETISSERHFANPHVEVVTDQVRTPSRSHPQA
                     WTIVHRKAAVIIAPMTRDGKIVLIRQERIPVRTAIWEMPSGQIDAVAAETIEQVALRE
                     LREEAGYQLAKDGKLIALGDYLTSPGFTDERGYFFLARPVEPIANYARDETESILDCR
                     AFSVPELRRMIAENEIRDANTLSMWTRLWARDLLPG"
     gene            complement(1048..2151)
                     /gene="argH"
                     /locus_tag="VE486_01825"
     CDS             complement(1048..2151)
                     /gene="argH"
                     /locus_tag="VE486_01825"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZA37857.1"
                     /translation="MRKGRFSKPAAKLAQRYTESVSFDWRLYRYDIAGSIAHAVALAR
                     RGILTADERQKIENELRAIEREIESGKFQWDQSLEDVHMNIEAALTKRIGTVGGKLHT
                     ARSRNDQIALDLRLYVKAEIVEVSNRLRNLQTALLNLAESRNGAIDPVMPGYTHLQRA
                     QPVTFAHYLLGQIEAFERDAERLRDCLTRADVLPLGSGALAGSTIVLDRERIARELGF
                     ARVSQNSVDAVSDRDFVCEFLFCLAMIGLHLSRLSEDLIIWSTSEFGFLEFSEAFSTG
                     SSLMPLSGEERNAFSRGARNRGQTRRVLRREPHFARSNSTRENERALTPFRYGCSDGF
                     RCSTFPFAATTIGAPSPENIAAQIKRWRKLLRE"
     gene            complement(2238..2618)
                     /gene="folB"
                     /locus_tag="VE486_01830"
     CDS             complement(2238..2618)
                     /gene="folB"
                     /locus_tag="VE486_01830"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZA37858.1"
                     /translation="MPNELNPFSDCIHIEQLEVSTRIGVPDKERATPQRLTVSISFWP
                     DHEAGDLADKIENAVNYSAIAEEAKNFARDQSVNLIETLADRLAMHLLKHFRMQKVTI
                     ELRKFPFEDAKYVSVTVTRTASAA"
     gene            2779..4620
                     /locus_tag="VE486_01835"
     CDS             2779..4620
                     /locus_tag="VE486_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA37859.1"
                     /translation="MKTVWRVFAYLKRYPWMAAGTLTCAILGTLMVIVFPSVTKWIID
                     DVVRANHPEKLFPLVLLAAVAFLLQHVFNAVRIILNNTFEQKVIFDLRSDLYSHIQML
                     PLRWFDNRATGDLMTRVIEDVNSVERVLIDGIEQGVVAVLQIVIVLAVMFYLNTTLAL
                     LALAPFPLLIAGALTYTLTAHRRYRLQRRSASAMNALLHDNLSGVRQIKSFVREQEEH
                     GRFNRVSDQLRRATLVVMRVWAIYSPSMSMFEAIGAVLVLGFGSHAVLTGAMQIGDLV
                     AFLMLTAFLYDPISRLHQLNQLVQAGRAAGERVFEILDEPAEPGFGVRRHVAGFKAQT
                     SLHTPNKRIAGDIRYEDVSFSYAEGLPALKQVSFHAPPGATIALVGATGAGKSTLVNL
                     LVRFYEFSGGQIYVDGRPIQEYALPRLREAIGVVTQESFLFNGSIRENLLMGKPDASD
                     AELWRAVDAANARGFIERLPAGLESIVGERGVKLSVGEKQRLSIARALLKDPPILILD
                     EATASVDTTTERLIQEALERLMFHRTSIVIAHRLSTIVHADQILVLDHGRIIERGTHD
                     ELVALGGKYARLCEQSLLEVSSSHAELESPAEIAPLPVPAPEEQLPV"
     gene            complement(4732..5211)
                     /locus_tag="VE486_01840"
     CDS             complement(4732..5211)
                     /locus_tag="VE486_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZA37860.1"
                     /translation="MKLAAIFRATFFSSALLLSAASAQTPSPEPVKTSDAAQLEALTK
                     KIDEQNAKIDALSQEILKLEQQISHIRPGVMIGESTPSASTGAAPAESPSHPMSGNTH
                     TVARGETLTSIAKMYKVSVDELQKANNIEDGRKLQAGQTIVIPGSPSPVPSSSPQTD"
     gene            5309..5485
                     /locus_tag="VE486_01845"
     CDS             5309..5485
                     /locus_tag="VE486_01845"
                     /inference="COORDINATES: protein motif:HMM:NF033388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HZA37861.1"
                     /translation="MPERIQKPVEPAPWGPKQGDGGGPRSPDVSRPDTKDLLKKMRKV
                     DPNQSKRYRQRTGQ"
     gene            5485..6318
                     /locus_tag="VE486_01850"
     CDS             5485..6318
                     /locus_tag="VE486_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37862.1"
                     /translation="MEPFLVAGDQNFSGGAIGDFPALLRRKGLLAPAAVVSSTPKAGT
                     PHEPSWQPTQATTILAFKFSGGVLVAGDRRATAGNTVVYDRADKVLEIDRNSIMAIAG
                     VPATAWEMARVLEHSFQYFRRTQLQEMSVDGKVRALSKLLRDNFGFVMQGVGVVVPIF
                     ATYDSHPKPEARLYFYDAMGAQFEATDYAATGSGSPAVRGILYYENNWGAKPLQKLNT
                     DEAVSIALRALDTAAESDTATGSVDRGGKIYPLIKIVSREGIVTLPEKKIAAVFRETV
                     A"
     gene            6315..>6949
                     /locus_tag="VE486_01855"
     CDS             6315..>6949
                     /locus_tag="VE486_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37863.1"
                     /translation="MIEEPYRWVEAIANRREYIEAQLASGSPITALGYRDGILFFTLG
                     QRRQKIFEIYDRIAMGAIGHPGDIERLRMAAIELASTEGFTRSATDVSLRRLVHYSLS
                     PLMKGAFEQVYGAPYLGRMLFAEVGEQPAEDLFLRVEYDGEIATNGATFAQARQDFAV
                     LSGTGQSRELMETFLKTEHRADASFETVLRTALDAWTIGHMTLQTNDAKELP"
BASE COUNT         1508 a         1856 c         2022 g         1563 t
ORIGIN      
        1 tgatcatggc gataacgagc gtgattgcca tcttcacgac ggtccatcgc aagtcttcga
       61 gatgctccag aaacggcttg gttgtctcgc tttcaggcaa ctcacgaaat ttgaaaatgt
      121 ctcgcaacgc catcagatca aaaattcgaa atccgaatgt cgaatgtcga aacaaaggcc
      181 aaaagcgcaa gtgtttgaaa aagcgatggt agagacggcg cgttgcgccg ggagtgcacg
      241 cttccagcgt gttcgcgtcg gcatcctgca gacgcatcgg tgttcggcaa gatgccgagc
      301 gtcgcacgca agatgcgtgc cctaccccgg aagaaggtca cgcgcccaga gccgcgtcca
      361 catgcttaaa gtattggcgt cgcgaatttc gttctccgca atcatgcggc gaagttccgg
      421 aacgctgaat gcgcggcaat ccagaatcga ttccgtttca tcgcgcgcat aattcgcgat
      481 tggttcaacc ggccgcgcca ggaaaaaata gccgcgttcg tcggtgaatc cgggggaggt
      541 aaggtaatcg ccgagcgcga tgagtttgcc atctttggcc agctggtaac cggcttcttc
      601 gcgcagttcc cgcaacgcga cttgttcgat tgtttccgcg gctacagcgt cgatctgacc
      661 cgatggcatt tcccagatgg cggtgcggac cgggatgcgc tcctgacgaa tgagaacaat
      721 tttaccgtcg cgcgtcatgg gtgcgataat gacggccgct tttcggtgca cgattgtcca
      781 cgcctgcggg tgggaacgcg acggtgttcg aacctgatcg gtcacgacct cgacatgtgg
      841 gttggcaaaa tgccgttcac tcgaaatggt ctcccaacct ttgccgtccc gcacgagatt
      901 gctccgctcc acgccggaag attatgaaac cagaaaggaa aagcgaatgc ttaggtggat
      961 cggccactcc gcggacgatg gtaaaataat gcggcttcgc cgccactatt ggcatcgagc
     1021 aagggactgc tcgatccacc tggcgactta ttcgcgaagc agtttgcgcc accgcttgat
     1081 ctgtgccgcg atgttttccg gtgacggagc gccgatcgtg gtcgctgcga aagggaaagt
     1141 cgaacatcga aaaccgtcac tacatccgta tcgaaaaggg gtgagagctc tttcattttc
     1201 gcgagtggaa tttgatctag cgaaatgtgg ttcgcgacgg agtacgcgac gagtttgccc
     1261 acgatttcgt gcgcctcgcg aaaaggcatt cctttcttca ccagatagcg gcatcaagct
     1321 ggaaccggtt gaaaatgctt cgctgaattc gagaaacccg aattcgctgg tgctccagat
     1381 gatcaagtcc tcgctcaaac gtgacagatg caaaccgatc attgcgagac aaaaaagaaa
     1441 ttcgcagaca aaatcccgat cgctcacggc atcgacgcta ttttgactga cgcgagcgaa
     1501 accgagctcg cgcgcgatgc gttcgcgatc gagaacgatt gtcgatccag cgagggcgcc
     1561 ggaaccaagc gggagaacat ctgctcgcgt caggcaatca cgaaggcgct ctgcgtcgcg
     1621 ttcaaaagct tcaatttgcc cgagcaagta atgagcgaac gtgaccggct gggcgcgttg
     1681 caaatgcgtg taaccgggca tcaccggatc gatcgcgcca ttgcgggact cggcaagatt
     1741 gagcagcgca gtctgcaaat ttcgcaaacg gttcgacact tccacaatct cggcttttac
     1801 ataaagccga agatcgagcg caatttgatc gttccggctg cgcgcggtgt gaagcttccc
     1861 gccaacggtg ccaattcgtt tcgtgagcgc ggcttcgatg ttcatgtgga catcttcgag
     1921 cgattgatcc cactggaact tacccgattc gatttccctt tcgattgcgc gcagttcgtt
     1981 ctcgatcttt tgccgctcat cggcggttag gattcctctc cgagcaagcg caacggcgtg
     2041 cgcaatcgat cctgcgatat cgtatcggta tagcctccaa tcgaaggaaa ccgattcggt
     2101 gtagcgctgc gctaatttcg cagccggctt gctgaatctt cctttccgca ttgttttttg
     2161 aatggcgacg atgccgggat caacgacccc ggctacaact tcctcttggc attgttaatt
     2221 cggacaagcg ctcatagtca cgcagcggaa gcagtgcgtg ttacggtcac ggagacgtac
     2281 ttcgcgtcct caaacgggaa ttttcgcaac tcgatggtga ccttctgcat tcggaaatgt
     2341 ttcagtaagt gcattgcaag ccgatcggcc aatgtctcga tcagatttac tgactgatcg
     2401 cgcgcgaaat tcttcgcttc ttcggcaatg gccgaataat tgacggcgtt ctcaatcttg
     2461 tctgccaggt ctcctgcttc gtgatctggc cagaaggaaa tactgaccgt cagtcgctgc
     2521 ggcgtggcgc gctctttatc aggaactccg atgcgcgtgg aaacctcgag ttgctcgatg
     2581 tgaatgcaat ccgagaacgg atttagttcg ttgggcaccc gcaaatctta accacgaata
     2641 gacacgaata aacacgaata ggagcaagaa agatccgccg caaataacgc tcgcgacgcg
     2701 aacatgttgt tttgatcgga atttcttttt cccgtgcatt cgcgtgattc gcgggcctct
     2761 ggtaacgttt tgtcctaaat gaaaacggtc tggcgtgttt ttgcctattt gaagcgttat
     2821 ccgtggatgg cggcggggac gctgacatgc gcgattctcg ggacgctgat ggtgatcgtt
     2881 tttccgtcgg tgacgaaatg gatcatcgat gacgtggtgc gggcgaacca cccggagaaa
     2941 ttgttcccgc tcgttttgct cgcggcggtg gcgtttctgc ttcaacacgt cttcaacgcg
     3001 gtccgcatta ttttgaacaa cacgttcgag caaaaagtga tcttcgatct gcggagcgat
     3061 ctttattcgc acattcaaat gttgccgctc cgctggttcg acaatcgcgc tacgggcgat
     3121 ttgatgacgc gcgtgatcga ggatgtgaac tcggtcgagc gtgtgctgat tgatggaatc
     3181 gagcagggtg tggtcgcggt tttgcagatt gtaatcgtgc tggcagtgat gttttatttg
     3241 aacacgacgc tcgcgctgct cgcactagcg ccgtttccgt tgctgatcgc cggggcgctc
     3301 acttacaccc tgaccgcaca tcgccgctat cgtttgcaac gccggtcggc ctcggccatg
     3361 aatgcgcttc tgcacgacaa tctttccggg gttcggcaga tcaaaagttt tgtgcgcgaa
     3421 caagaagaac acgggcgctt taatcgggta agcgatcagc tgcgacgcgc cacgctggtc
     3481 gtgatgcggg tctgggcgat ctacagcccg tcaatgtcga tgtttgaggc aatcggcgcg
     3541 gtgctcgtgc ttgggttcgg cagtcacgcc gttttgacag gcgcaatgca aatcggcgat
     3601 ctggtcgcat tccttatgct cacggcattt ctctacgatc caatcagccg gttgcatcag
     3661 cttaatcaac ttgtgcaagc cggacgcgcc gctggcgaac gggtgtttga aattctggat
     3721 gaaccagcgg agccaggatt tggagtgcgg cgacatgtcg ccggtttcaa agcgcagaca
     3781 tctctgcaca ctccaaataa gcgaatcgcg ggcgacattc gctacgaaga cgtgagcttc
     3841 agttacgcgg aaggattgcc cgcgctcaaa caggtttctt ttcatgcgcc gccgggcgcc
     3901 acgattgcgc tggtgggggc aaccggcgcg ggcaaatcga ccctggtgaa tttacttgtt
     3961 cgattttacg aattcagcgg tggacaaatt tatgtcgatg gaaggccgat ccaggaatac
     4021 gcgctgccga ggctgcgtga agcgatcggc gtggtgacac aggaaagttt tttgttcaac
     4081 gggtcgattc gagagaacct tttgatggga aaaccggatg cgagcgatgc ggaattgtgg
     4141 cgcgccgtgg acgccgctaa tgcgcgggga ttcatcgagc gattgccggc tggcttggaa
     4201 agcatcgtcg gcgagcgcgg cgtgaagttg agcgtcggcg agaaacagcg actgtcgatc
     4261 gcccgcgcgc tgctgaagga tccaccaatc ctgatcctcg atgaagcaac ggcgagcgtg
     4321 gacacgacga cagaacgact aatccaagaa gcgctcgagc gcctgatgtt tcaccggacg
     4381 tcaatcgtga tcgcgcaccg gctttcgaca attgtccacg ccgaccaaat tctcgtgctc
     4441 gatcatggcc gaattatcga acgcggcacg catgatgaat tggtcgcgct gggtggaaaa
     4501 tatgcgcggc tctgcgagca gagcttgctc gaagtctcgt cgtcccatgc tgaattggaa
     4561 tcgccagcgg agatcgcccc tttgccggtt cccgctcctg aggagcaatt gccggtctag
     4621 ttccggagct cagaaaaccc gccaatcaaa atttggacgg gcctcccgcc ttcgatcgcc
     4681 gcggcgacct acggcgtgac aaacagggcc cgcgctacaa atgctcctgc gtcaatcggt
     4741 ttgcggggaa cttgaaggca ccggcgaggg cgagcctgga atcacgatgg tttgtccggc
     4801 ctgcaattta cggccatctt cgatattatt tgccttttgc aattcgtcca cgctcacctt
     4861 gtacatcttc gcgatggagg tgagtgtctc cccgcgcgcg accgtatgag tgttcccgct
     4921 catcgggtgc gacggagatt ccgccggcgc agcaccggtg gatgctgacg gcgtgctttc
     4981 gccaatcatt acacctgggc ggatatgcga gatctgctgc tctaacttga ggatttcctg
     5041 cgagagagcg tcaatcttcg cgttctgctc gtcgattttc tttgtcagtg cttcgagttg
     5101 agcggcgtca gatgttttaa cgggctcggg cgaaggcgtc tgtgccgaag ctgccgaaag
     5161 caacaaggcg ctggaaaaaa aggttgcacg gaaaatagca gccagcttca tggtcccaaa
     5221 cgttggcatt agcggctttg tctggcaagc agcgaggcgg atacagatgc gcagcttgcc
     5281 tttccaacat ttgctcttaa atttttttat gcctgaacgc atccaaaaac cggtcgagcc
     5341 agctccgtgg ggccccaagc aaggcgacgg tggcggcccg cgttcaccgg atgtctcgcg
     5401 accggacacg aaggatctat taaaaaaaat gcgcaaggtc gaccccaacc aatccaagcg
     5461 ctatcgccag cgcaccggtc aataatggag ccattcctgg tggcgggtga ccagaatttt
     5521 tcgggcggcg caatcggcga cttccctgcg ctgctgcgac gcaaaggttt gctcgctccg
     5581 gcggcggttg ttagttcaac accaaaggct ggaacgccgc acgagccttc gtggcagccg
     5641 acccaggcga cgacaattct cgctttcaaa ttctcgggtg gcgtgctggt ggcgggcgat
     5701 cggcgcgcga ctgccggcaa cactgttgtt tacgatcgtg ccgacaaagt tttggagatc
     5761 gatcgcaatt cgattatggc catcgccggc gtccccgcta cggcctggga aatggcgcgc
     5821 gtgctcgaac attcatttca atactttcgg cgaacccagc tccaggaaat gagcgttgat
     5881 ggaaaagtgc gcgcgctgtc caagctcttg cgcgacaatt tcggatttgt gatgcaggga
     5941 gtcggagtcg tggtgcctat tttcgcaact tacgattccc atccaaagcc cgaggcgcgg
     6001 ctttattttt acgatgccat gggagcgcaa tttgaggcga ccgactacgc cgcaaccgga
     6061 tcagggtccc ctgccgtgcg cggcattctc tattacgaaa ataattgggg cgcgaaaccg
     6121 ttgcaaaagc tgaatacaga cgaggcggtt tcgattgcat tgcgcgcgct ggatacggct
     6181 gcggaatccg acacagcgac gggcagcgtg gatcgcggcg gcaagattta tccgctcatc
     6241 aagattgtat cgcgcgaggg catcgtgacc ttgccggaga aaaaaatcgc cgccgtcttc
     6301 agggaaacgg ttgcatgatc gaagagccgt atcgttgggt tgaggcgatc gccaatcggc
     6361 gggagtacat cgaagcgcag ctcgcttcgg gcagtccgat cacggcactt ggttatcgcg
     6421 acggtatttt atttttcacg ctcggccaga ggcgacagaa aatattcgag atttacgatc
     6481 ggattgcgat gggtgcgatc gggcatccgg gagatattga gcgattgcgc atggcagcca
     6541 ttgaacttgc cagcaccgag ggtttcacgc gttcggcgac agatgtctca ctgcggcggc
     6601 tggtgcatta ttcgctcagt ccgctgatga agggcgcatt cgaacaagtg tacggcgcac
     6661 cctatctggg gcggatgctt tttgctgagg tgggcgagca accggcggaa gatttgtttc
     6721 tgcgggtgga atacgatggc gagatcgcaa ccaacggcgc aacctttgca caagcgcggc
     6781 aggatttcgc cgtcctcagc ggaacagggc aatcgcggga gctgatggag acatttctca
     6841 aaaccgaaca tcgagcggac gccagtttcg aaacggtgct gagaaccgcg ctcgacgcat
     6901 ggactatcgg tcatatgaca ttacagacga acgatgcgaa ggaactgcc
//
LOCUS       DASQUQ010000066         9483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_553526, whole genome shotgun sequence.
ACCESSION   DASQUQ010000066 DASQUQ010000000
VERSION     DASQUQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553526"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..2150
                     /locus_tag="VE486_01860"
     CDS             297..2150
                     /locus_tag="VE486_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HZA37864.1"
                     /translation="MSREFYYRWEWPLRSSPDALWPLVADTNRFDRDVGFPSVERLPK
                     AGGDDRKPGHRVRTKIGGIRIEWDERPFEWVRPQQFEVVREYLSGPLKNLRVLVKLMP
                     GTAGGTQLVYQIWLQPRGLLGALLIPLQARIAKRRFGKTFRHYDNLALVGRTPADSPM
                     AAPAACKPYIAPQGEKRLEAAARRLVEQGLPSQLTEKLATFILTADEMDLTHIRPYAL
                     ADQWRVPRRDVLNLCLHATRSGVLDLQWDVLCPLCRGAQESNSTLRQIHPQTHCESCN
                     VDFTVNFERSVELTFRPNPAVREVVARQYCVGSPNVTPHILAQQVLAPGTAREMSLAL
                     EPGRYRLRATSLKGGRSFVVAAKGADKIEFRASADAWPDDEPQIMLRPTLRLVNATGL
                     EQPFLIERMAWTEQAATAAEVIALQVFRDLFSTEALRPGEQISVGTLAVVFTDLREST
                     RLYRQIGDAPAFGHVMNHFDILRRAIDAEGGALVKTIGDAVMAVFRRPLTALKAVLRA
                     QKELDELGAPPLMLKAGIHTGPCIAVTLNERLDYFGSTVNLTARIQRFSEGGDVVISA
                     AMRWDPEVSEFLEKSCSVRVEPFASPLKGFDKDRFELWRVKPVSSTSSSNR"
     gene            2412..3410
                     /gene="hprK"
                     /locus_tag="VE486_01865"
     CDS             2412..3410
                     /gene="hprK"
                     /locus_tag="VE486_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HZA37865.1"
                     /translation="MPEQRGTQTITSTSDQQKSVPVVTVEGFYDSHAEKLQMKLEGPR
                     VGFHRKIREPTINRPGLALSGFYTYFAEKRVQVLGAAEHSYLKSLAPRARVQRFRALC
                     ARKIPCLVVSRGFHLDPSLLAVAAEEKIAVFRTPMITMKFINAATIALEVDFSPTVTE
                     FGSMVDILGVGVLIRGASGIGKSECVLGLIERGYSLVADDVTRITSLEGRELMATAPD
                     LTRNHMEVRGIGIINVASVFGIGSIRVEKRLDLVATLKDWQEVEAVDRIGLDRAFYEI
                     LGLQVPHVTIPVRPGRDMARLIEVAAMDQKLKDLGENSAVAFNTKLLNLMDQKGPM"
     gene            3410..3721
                     /locus_tag="VE486_01870"
     CDS             3410..3721
                     /locus_tag="VE486_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZA37866.1"
                     /translation="MGLLSRRANRAASGQKVEKEITIVNRLGLHARPAAMFVRIASRY
                     RSEIWVSKEGEEVNGKSIMGLMMLAAGQGSKLRIRCEGPDAQKAMEELKELMNARFNE
                     D"
     gene            3821..5587
                     /gene="ptsP"
                     /locus_tag="VE486_01875"
     CDS             3821..5587
                     /gene="ptsP"
                     /locus_tag="VE486_01875"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZA37867.1"
                     /translation="MSDTARQEIRFEGAGVSPGIAHGKVHVVRDDLDDVPRYRIAPSQ
                     IADEIGRFEAALIQTRMQILEMQQRIAESIGAKDAAIFDAHLLVVEDRTLIDEVLRKL
                     ETDLCNVEWIFQEVATRYAETLNKIDDPYLRERAVDIQDVTKRVIRNLQGKAPKKFLG
                     LTEPHILIAHDLTPSDTASMKREHVLGIATDLGSRTSHTAIMARSLGIPAIVGLHDIT
                     TKLETSQEVLLDGTDGLLILDPTPETLAHYADVESKRARVVAQLKELRETSSTTRDGR
                     HIVLSANIELPEDVEAVAANGAEGIGLYRTEFLYLNRTTLPTEEEQYEIYRKVAERVR
                     PDPLIIRTFDLGGDKLAPGTVDITDELNPFLGWRAIRLCLENIDIFKTQLRAILRASV
                     VGNIKIMFPMISGLDELRGAIAVLAECKDELCSSGIDIGEEVEVGAMIEIPSAAISAN
                     VLAREADFFSIGTNDLIQYALAVDRVNEKIAHLYEPAHPAILRLLKMIADAAHANDIW
                     VGVCGEMAGDVALIPLLLGLGMDELSVSATLVPRVKRAVQSLAIPECRELVEVALNLD
                     TASEILARCLELADKRYGDLLG"
     gene            complement(5686..6162)
                     /locus_tag="VE486_01880"
     CDS             complement(5686..6162)
                     /locus_tag="VE486_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HZA37868.1"
                     /translation="MNKAVIEVQVRAVLPTSGGCAVFIGNNDKVFIIYVDQTVGSAIT
                     MFMRQITKERPLTHDLMGHLMTALGAKVDRVIINDLKNATYYARVIIRVENELQQKKI
                     IELDGRPSDCIAMATQQKAPIYVSQDVWDEVDDMSDVLRKMEEEGLKPDPETEAEE"
     gene            6357..8312
                     /gene="speA"
                     /locus_tag="VE486_01885"
     CDS             6357..8312
                     /gene="speA"
                     /locus_tag="VE486_01885"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HZA37869.1"
                     /translation="MKTEWDLESAIATYNVEGWGNGYFTVNSSGNVEAKPLQDNGGSI
                     DLLEVVNEARARHLGFPLVIRFQDLLRHRVESINRAFQTAITEFGYRNEYRGVFPIKV
                     NQLREVIEEIVDAGQPFHFGLEAGSKPELVAALAMHQDPESLIICNGYKDPAFIRIAL
                     LGRKLGKSVVIVVEKLEELEQTIRISNEVGVEPFIGIRVRLHSKGSGKWSPSGGENAK
                     FGLDTTNLVAASQMLKEAGLAHCFKLVHFHVGSQVPDISTIKRAVREAARYYAKLCKL
                     GHELSYLDVGGGLGVDYDGSRSDFDSSTNYSLQEYANDVVWNIMDVCDSEGVTHPAIV
                     SESGRAIVAHHSVLVMEAFSSIERTAPKLKVEATEKDHRLVHDILEVKQRLKRGNRIE
                     SLHDSQHIKEESQQMFDLGLLDLESKAKIETVCWQLAQQIVTMHRGLRFVPEEVKQLE
                     TSLGDQYICNFSVFQSLLDHWALGQLFPIMPIHRLDTPPDRNGMIVDITCDSDGKISK
                     FIDLQDVKETLPLHRIPPGEMYYIGVFMVGAYQDIMGDLHNLFGRVTEVHVFLDPDEE
                     SGWYIEEVIEGSTIGEVLAMTQWDKVELMRLLKSQVDDAIKTDRLKPSDAMKLLSDYE
                     RLLQEYTYLSFNGAKVQSQSGNWLPLS"
     gene            8314..9156
                     /locus_tag="VE486_01890"
     CDS             8314..9156
                     /locus_tag="VE486_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37870.1"
                     /translation="MPGETCRAFKALLLIVVCQASFVARLFAEPLAKGIQDNSFFVEE
                     AYNQEPGIVQHIINLPVFFMDGEKEITPSFTQEWPIFSQTHQFSYTIPYTFTEHENGF
                     EDIRLNYRLQALMEGERTPAFAPRFSLVTPAGDTNKGFGHGRLGYEINLPFSKIVSNR
                     VTLNFNAGGSLFPDVNDRDLWNYNLGSSAIYAVSRDFNLMLEAVAVWEEDVDSTRRID
                     RTVTALLSPGARYAFNCPNDLQIVVGAATPVGLTSDSPDWGLFFYVSFEHPFVRLQPA
                     DGKD"
     gene            complement(9180..9251)
                     /locus_tag="VE486_01895"
     tRNA            complement(9180..9251)
                     /locus_tag="VE486_01895"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9216..9218),aa:Phe,seq:gaa)
BASE COUNT         2127 a         2563 c         2672 g         2121 t
ORIGIN      
        1 gtcttgagcg tggaggatgt gggagagaac ggtgatgcga aaaatgagtg accggacaaa
       61 cgcgagcggc tttaatgcac tgcacaatgc gttcactgaa ttcgctgatg tgcagcgcgc
      121 gcaatttgaa gccgcgccgc ctgcgagctc gcccgatggc gcgacgatcg aggatgacct
      181 gcgaatcttg cgaacgggca caggaaatac gttttgatat ttggagagta attaagacgc
      241 ccggtttcgg ccatgggcaa tagcaaggct tgccacttca tagtgcgatc actgggatga
      301 gccgggaatt ttactaccgc tgggaatggc cgctccgctc cagcccagat gcgctttggc
      361 ctttggtggc cgacacgaac cgctttgatc gagatgtggg ttttccgtcg gtcgagcgct
      421 tgcccaaggc cggcggggac gatcgcaaac ccggtcatcg cgtgcgcacc aagatcggcg
      481 gtatccgcat agaatgggat gaaaggccgt ttgaatgggt gagaccgcag cagttcgaag
      541 tcgtccgcga atatctctcc ggtccgctca aaaatctgcg tgtgctggtc aaactaatgc
      601 cgggaacggc tgggggcaca cagctcgttt atcagatatg gttgcagcca cgtggccttc
      661 tcggcgcgct ccttattccc cttcaggctc gaattgcgaa gagacgcttc ggcaaaacat
      721 tccgccacta tgacaatctt gccttggtgg gccgcacgcc tgctgattcg ccaatggccg
      781 cgccagcagc atgcaagcct tacattgctc cccagggaga aaagcggctg gaggcggcgg
      841 cccgcaggct ggtcgagcaa ggcttgccgt cacagctaac cgagaaactc gcaacattca
      901 tcctgaccgc tgatgagatg gatttaacgc acatccggcc gtacgcgttg gccgatcagt
      961 ggagggtgcc gcggcgcgat gtgctcaatc tttgcctcca cgccacgcgc tctggcgtgc
     1021 tcgatttgca atgggacgtg ctctgcccgc tttgccgcgg cgcgcaggaa agcaactcta
     1081 cgcttcgaca gattcatccc cagacgcatt gcgaaagttg taacgttgac ttcaccgtga
     1141 atttcgagcg ctcggtggaa ttgacgtttc gcccgaatcc ggccgtgcgc gaagtggtgg
     1201 cgcgccaata ttgcgtgggc agtccgaacg tgacgccaca catcctggcg caacaagtgt
     1261 tggcgccggg aaccgcacgc gaaatgtcgc tggcattgga accgggacgc taccgattgc
     1321 gcgccacatc gctgaaagga ggacgatctt tcgtcgtcgc ggcgaaaggt gcggacaaaa
     1381 ttgagtttcg cgcgagcgct gatgcctggc cggatgacga accacagatc atgctccgcc
     1441 cgacgttgcg attggtaaat gccaccggct tggaacagcc ttttttgatc gaacgaatgg
     1501 cgtggaccga acaggccgcg accgcggcgg aagtaatcgc attgcaagtt ttccgcgatc
     1561 ttttctctac cgaagcgttg cggccgggtg aacaaatttc tgtcggcact ctcgccgtcg
     1621 ttttcactga tctgcgtgaa tcgacgcggc tttatcggca gatcggcgat gcccccgcat
     1681 ttggtcacgt catgaatcac ttcgatatat tacgtcgagc gattgacgcc gaaggtggcg
     1741 ccctcgtgaa aacaatcggc gacgccgtca tggccgtgtt tcgccgtccc cttactgcgc
     1801 tcaaagccgt cttgcgcgcc caaaaagaac tcgacgagtt gggagcgcca ccactcatgt
     1861 tgaaagccgg cattcacacc ggcccctgta ttgccgtaac gctaaacgaa cgtctcgatt
     1921 acttcggttc gactgtgaac ctcactgcgc gcatccaacg tttttccgaa ggcggagacg
     1981 tagtgatttc cgctgcaatg cgttgggatc ctgaggtttc ggagtttttg gaaaaatcat
     2041 gctccgtgcg cgttgagcct ttcgcctcgc cgctgaaagg tttcgataag gaccgcttcg
     2101 aactctggcg tgtgaaaccc gtgtcatcga catcgagctc gaaccgatga tcgaaagcgc
     2161 aattcccatt cgcaatggga agtttatgcg gagcgaagaa ccgctcagta tcgagatgtc
     2221 gactgcacgc gagagagaac gtcgaaaaac ctttccagga gctgtcctac aacagcaatg
     2281 acaaccgggc gaaatcatcc cattgaatgt agcttttcac gctgcagagt aggcgcttcg
     2341 cctgtcaaac ttgcgtcaca acatgctgca gattggaaat tgcgcgttat tcacgctaga
     2401 agttaatcct gatgccggaa caacgcggga cccagacgat tacatcgaca agcgaccaac
     2461 aaaaaagcgt tccggttgtc acagtcgaag gcttttacga ttcgcacgcc gaaaagcttc
     2521 aaatgaaact ggaaggtccg cgagtgggat tccatcgcaa aattcgcgag cccaccatca
     2581 accgccccgg tctggcgctt tcggggttct acacctattt cgcggagaaa cgggtgcagg
     2641 tgctcggtgc ggctgagcac tcgtatttaa aaagcctcgc tccgcgagcg cgcgtgcagc
     2701 ggtttcgcgc cttgtgtgcg cgtaaaattc cgtgtctcgt cgtctcgcgc ggattccatc
     2761 tcgacccttc actgctcgcc gttgccgcgg aagagaaaat cgccgtgttt cgcacaccga
     2821 tgatcacgat gaagttcatc aatgccgcga ccatcgcgct ggaagtcgat ttctcgccta
     2881 ccgtaaccga gttcggcagc atggtggaca tcctgggcgt cggcgttttg attcgtggcg
     2941 cgagtggcat cggcaagagc gaatgcgtcc tcgggctgat cgagcgcggc tatagcctgg
     3001 tcgctgacga cgtgacacga atcacttcgc ttgaagggcg tgagctcatg gcgacagcgc
     3061 cggacctgac ccgcaatcac atggaagttc gcggaattgg cataattaac gtggcgtcag
     3121 ttttcggcat tggcagtatc cgagtcgaga agcgcctcga tctagtcgct acgctgaagg
     3181 attggcagga agtcgaggcg gtcgatcgga tcggcctcga tcgggcgttt tacgaaatcc
     3241 ttgggcttca ggtgccgcac gtgacaatcc cggtgagacc gggtcgcgac atggcgcgtc
     3301 tgatcgaagt tgccgcgatg gatcagaaat taaaggattt gggcgaaaat agcgcggtcg
     3361 cgttcaatac gaagctgctc aatctaatgg atcagaaggg accaatgtaa tgggcttgtt
     3421 gtctcgaaga gccaaccgcg cagcgtccgg ccagaaggtc gagaaggaga ttacaatcgt
     3481 gaatcggctt ggtctccatg cgcggccggc ggccatgttt gtgcgcatcg cgagccgcta
     3541 tcgttcggaa atttgggtgt cgaaggaagg ggaagaagta aacgggaaaa gcatcatggg
     3601 cctgatgatg ctggccgcgg gacagggatc aaaactgcgc attcgctgcg agggcccgga
     3661 cgcgcagaag gcgatggagg agctcaagga gctgatgaac gcgcgcttca acgaagattg
     3721 aggcatccca ttggacgtat atgacccatg ggacgtatgc gccgcgttct gtcaggcaga
     3781 ctgttcctga gttgacatcg ccattactct gagaattgga atgagcgaca cggcacggca
     3841 ggaaattcgg tttgaaggag cgggggtttc gccgggaatt gctcatggca aggtccatgt
     3901 cgttcgagat gacctggatg acgtgccgcg ttatcggatc gcgccttccc agatcgcgga
     3961 cgaaatcgga cgcttcgaag cggcgctcat ccagacgcgg atgcagatcc tagagatgca
     4021 gcagcgcatc gcggaatcaa tcggagcaaa agatgccgcc attttcgacg cccatctgct
     4081 cgtagtcgag gatcgcactt tgatcgacga agtgttgcgc aaattggaga ccgacctttg
     4141 caatgtcgag tggatatttc aggaagtcgc tacgcgttat gctgagaccc tgaacaaaat
     4201 tgacgatccg tatttgcgtg agcgcgccgt tgatattcag gacgtaacca agcgagtaat
     4261 ccgtaatctc cagggcaaag ctcccaagaa atttctcggt ttaactgagc cacacattct
     4321 catcgcccac gatcttactc cttccgacac tgccagcatg aagcgggagc acgtgctcgg
     4381 gattgcgacg gatcttggca gccgcacgtc gcatacagcg attatggcgc gctcactcgg
     4441 tattccggcg atagtcggct tgcacgacat cacgacaaaa ctcgagacta gtcaggaagt
     4501 gctcctggat gggacggatg gtttgctcat tctcgatccg acgccggaaa cgcttgcgca
     4561 ctacgcggac gtcgaatcaa aaagggccag agtcgtagcg caactcaaag agctgcgcga
     4621 gacgagttcg accacgcgcg atggccggca cattgtcctg tctgcaaata tcgagcttcc
     4681 ggaggacgtt gaagcagtcg cagccaatgg cgcggaggga atcggacttt atcgcacgga
     4741 gttcctttat ttgaatcgca ccactttgcc gaccgaggag gaacaatacg aaatctatcg
     4801 aaaagtggcg gagcgggtgc ggcctgatcc gctgattatt cgaacatttg atcttggggg
     4861 cgacaagctg gctcccggca ctgtcgatat taccgatgag ctaaacccat ttctgggctg
     4921 gcgcgcgatc cgtttatgcc tcgagaacat cgatatattc aaaacgcagt tgcgcgccat
     4981 tctgcgcgcc agtgttgtcg gtaatataaa aatcatgttt ccgatgatct ccggcctgga
     5041 tgaattgcgc ggcgccatcg ctgtgctcgc cgagtgtaaa gatgagctgt gcagctcggg
     5101 gattgacatc ggcgaagaag tcgaggtcgg cgcgatgatc gaaatcccaa gtgccgccat
     5161 atcggccaat gtgcttgctc gcgaggccga ttttttcagt atcggaacaa acgatctgat
     5221 tcagtacgcg ctggcagtcg atcgcgtgaa tgagaagatc gcgcatcttt acgaaccggc
     5281 tcatccggcc atcctgcgtt tgctcaaaat gatcgccgat gcggcgcatg cgaacgatat
     5341 ctgggtcggc gtttgcggag agatggctgg ggatgtcgcg ctcattcccc ttttgctggg
     5401 gcttgggatg gacgaattga gcgtaagcgc aacgctcgtt ccgcgagtca agcgcgcggt
     5461 ccaaagtctg gccattcccg aatgccggga attggtcgag gtggcgctca acctcgatac
     5521 tgcgtcggag attctggcgc gttgtctgga attagcggac aagcgctacg gggacttgct
     5581 cggctgaagc ggcgttaaaa acattacgac gttacaaaag ctaccagagg aaaatttctg
     5641 atgaagtttt cacgatgtaa ccttgtaacg cttttaacgg attacttact cttccgcttc
     5701 cgtttccgga tcgggtttta gcccttcttc ttccatcttg cgcaagacat cgctcatatc
     5761 gtccacttcg tcccagacat cctgactcac ataaatagga gccttttgct gagtggccat
     5821 tgcgatgcaa tcgctcggcc ggccatccaa ctcgatgatc tttttctgct gcaattcgtt
     5881 ttcgacccga atgatgaccc gggcgtagta agttgcattt ttcagatcgt tgataatcac
     5941 ccggtcgact tttgcgccga gagctgtcat caagtgcccc atcagatcgt gggtgagggg
     6001 acgttccttg gtaatctgac gcatgaacat ggtgatcgcg ctgcccaccg tttgatcgac
     6061 ataaatgatg aacaccttgt cgttgttacc gataaacacc gcgcaccccc cactcgtcgg
     6121 caataccgcc ctcacttgca cctcgatcac cgctttgttc atcacgcgaa tggtacccgt
     6181 gcgagcatga aagacaagcg agggccatgc ccgcttcgtt gcggcgaagc cgacatcgtc
     6241 gctctttcgc gctcacacga atagtttttt agtgaaaatg ttttgaagcg ggcttgcatg
     6301 ttggacgcgc tgattgaata tgcgctttgc ggttcgcagc cgcgtgatga atcgaaatga
     6361 aaaccgaatg ggacctggag tccgccatcg ccacctacaa cgtggaagga tggggcaatg
     6421 gctactttac ggtcaatagt tcaggcaacg tcgaggcaaa gccgttgcag gataacggcg
     6481 gttccataga cctgctcgag gttgtaaatg aagcgcgcgc gcgccacctc ggttttccgc
     6541 tcgtaatccg atttcaggat ttgctccggc accgggtcga atcaattaac cgggcctttc
     6601 aaacggcgat tacggagttc ggttatcgca acgaatatcg gggcgttttt ccgatcaagg
     6661 tcaaccagtt gcgcgaagtg attgaggaaa tcgtcgatgc aggccaaccg tttcactttg
     6721 gcctcgaagc gggcagcaaa ccggaactgg tcgcagcgct cgcaatgcac caggaccccg
     6781 agagcttgat catctgtaat ggctacaaag atcctgcttt tattcgcatt gcccttctcg
     6841 ggcgtaagct tggcaaatcg gtcgtcattg ttgttgaaaa gctagaagag ctcgagcaga
     6901 ccatccgcat ttcgaacgaa gttggtgtcg agccgttcat cggcattcgt gtgcgcttgc
     6961 atagcaaagg gtctggcaaa tggtctccca gcgggggcga gaacgcgaaa ttcggtctcg
     7021 ataccaccaa cctcgtagcg gccagccaga tgttgaaaga ggccggtttg gcgcactgtt
     7081 tcaaacttgt tcatttccat gttggatcgc aggtgccaga catttcgacg atcaagcgcg
     7141 cagttcgcga agcagcgcgc tattacgcga agctgtgcaa gcttggacac gagctcagct
     7201 atctcgatgt gggcggcggt ctcggggtgg attacgacgg gtcacgcagc gatttcgaca
     7261 gctctaccaa ttattcgctc caggaatatg ccaacgacgt tgtctggaac atcatggacg
     7321 tgtgcgattc agagggcgtc acgcatccgg ccattgttag cgagagcggg cgcgccatcg
     7381 tcgcgcacca ttccgttctg gtaatggaag ctttcagctc gatcgaacgg accgcgccca
     7441 agctcaaagt cgaggctacc gaaaaagatc acaggctggt tcatgatatt ctcgaggtga
     7501 aacaacggct caaacgtggc aaccgcatcg agagcctgca tgacagccag cacatcaagg
     7561 aagaatcgca gcagatgttc gatctcgggc tgctcgatct cgaatccaag gcgaagatcg
     7621 aaaccgtttg ctggcagctc gcgcagcaga tcgtgactat gcatcgcggt ctgcgctttg
     7681 tgcccgagga agtaaagcag cttgaaactt cactgggcga tcaatacatc tgcaactttt
     7741 ctgttttcca atccctgctt gaccattggg cgctcggcca gcttttcccg atcatgccga
     7801 tccatcggct cgacactccg ccagatcgca acggcatgat cgtcgatatc acctgcgatt
     7861 ctgacggaaa gatctcgaaa tttatcgacc ttcaggacgt aaaggaaaca ttgccgctcc
     7921 atcgcatccc gcccggcgaa atgtattaca tcggcgtttt catggtaggc gcttatcagg
     7981 acatcatggg cgaccttcac aatttgtttg gccgcgtcac ggaggtgcat gttttcctcg
     8041 atcccgacga agagtcgggt tggtacattg aggaggtaat cgagggcagc accatcggcg
     8101 aggtgcttgc catgacccag tgggataaag ttgaactgat gcggctgctc aagtcgcaag
     8161 tagatgacgc gatcaaaacc gatcgactca agccaagcga cgcgatgaaa cttctctccg
     8221 actacgagcg tcttctccag gaatatactt atctcagttt taacggggcc aaagtgcaat
     8281 cgcaatctgg caactggttg ccgctgagct aacgtgccgg gagaaacctg ccgggctttt
     8341 aaggcccttc ttctgatcgt cgtctgtcag gcgtcattcg tcgcccggct cttcgccgag
     8401 ccgttagcaa aaggaattca agacaactct ttcttcgtcg aggaggccta caaccaggaa
     8461 cccgggattg ttcagcacat catcaatctc ccggtattct tcatggacgg cgaaaaggaa
     8521 attacaccga gtttcaccca ggaatggcca atcttcagtc agacccacca gttctcctac
     8581 acgattccgt acacgttcac tgaacatgag aacggtttcg aagacatccg cctcaattac
     8641 cgattgcaag cgctgatgga aggtgaacgc acgcccgcat tcgcgccccg cttttctctt
     8701 gtcacgccgg ccggcgacac caacaaaggt ttcggacacg gccggctcgg ctacgaaatc
     8761 aatttaccat tcagcaaaat tgtcagtaac cgcgtaacgt taaattttaa cgccggtggt
     8821 tcgcttttcc ccgacgtaaa cgatcgagac ttatggaatt acaatctcgg cagcagcgcg
     8881 atctacgcgg tgagcaggga tttcaacctg atgctcgaag cagtcgctgt ttgggaggaa
     8941 gacgtcgatt ccacaagaag gatcgatcgc accgtcacgg cgcttctttc tcccggtgcg
     9001 cgttacgcct tcaattgccc gaatgatttg cagatcgtcg tcggtgccgc cacgccggtc
     9061 ggtctcacgt ccgattcacc agattggggg ctattctttt atgtctcctt cgagcatcca
     9121 tttgtgcggc tgcaaccggc cgatggcaaa gattgattcg ctcaccgtta agaaaaattt
     9181 ggctggggtg gaatcgagcc gcctacacgt ggattttcgg tccactgctc tgccaagtga
     9241 gctatccagt cgtcgctgat gccgatcttg tgtccgcgtc gcgcggccta cgtgaacccg
     9301 atcatggctc tgcggacgtg atagcacggc gaattcgcgc cgcttcccgc ataaagttcg
     9361 gatttcggat tatctcagtg ggttttgttt gttgagatcg gtcccgcacg aagaaggaaa
     9421 agcaatcgat caatcgacgc gcacgccagt tgatcagctc ctgcctcaca caatgcagcc
     9481 cgt
//
LOCUS       DASQUQ010000067         5333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_646601, whole genome shotgun sequence.
ACCESSION   DASQUQ010000067 DASQUQ010000000
VERSION     DASQUQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646601"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VE486_01900"
     CDS             complement(<1..180)
                     /locus_tag="VE486_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37871.1"
                     /translation="MKKDDFDYAIENTQVILAPEQQIATFGSTSFHFYLISELMDRVD
                     QVRIRNGKIHAERPQI"
     gene            complement(260..1246)
                     /locus_tag="VE486_01905"
     CDS             complement(260..1246)
                     /locus_tag="VE486_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZA37872.1"
                     /translation="MPPAFLEIQDLQTHFPVEAGLIFKRRLGMVRAVDSVSLSINEGE
                     VLGVVGESGCGKSTLGRTIVQLIPPTSGTVVLEGRNLNQLRGRELRRARSDFQIIFQD
                     PYASLNPRMNVFDTLAEAMRTHKKVPRRELRQRVAALMRKVGLLPRTMRKYPHEFSGG
                     QRQRIAIARALAVEPKLIIADEPVSALDVSIQAQIINLLAQLRHEMRLTLLFISHDLS
                     VVKHISDRIAVMYLGKIVELGEARAVLEKPLHPYTKALISAVPIVDPTREHARKRIVL
                     PGDPPSPMNPPVGCRFHPRCPYMIEACKAQVPPLVDWGEKHFAACIRVPEIN"
     gene            complement(1328..1945)
                     /gene="msrB"
                     /locus_tag="VE486_01910"
     CDS             complement(1328..1945)
                     /gene="msrB"
                     /locus_tag="VE486_01910"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZA37873.1"
                     /translation="MYGRNLQYKHGSHKTLWIALISAVVVVVGSILFGYAQGQKKQAE
                     KMNPMKEIPSDAELRRHLSKDQYRVTREGGTETPFQNAYWDNHQPGLYVDIITGEPLF
                     SSLDKFDSGTGWPSFTKPISEDKVVEKRDSSHGMERTEVRSAKSDAHLGHVFDDGPPP
                     TGERYCLNSAALRFIPAEKLKEEGYGQYLSLFQSQPGGEQQRQQQ"
     gene            complement(1996..3018)
                     /locus_tag="VE486_01915"
     CDS             complement(1996..3018)
                     /locus_tag="VE486_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA37874.1"
                     /translation="MAELLRVENLRTYFHTRAGIVRAVDGVSFSVNPGETLGIVGESG
                     SGKSVACLSLLRLIPSPPGRIESGRADFNGIDLLHCDDEQLRKIRGHRISMIFQDPMS
                     SLNPYMRIGNQVMEPLLIHERLSRAAARQRALAALREVGIPEPERTMRSYPHEFSGGM
                     RQRVMIAAALITKPAVLIADEPTTALDVTVQAQILELIRKMQSELGTAVIFITHDVGI
                     VSGFCDRVLVMYAGRIVESASTARIFYEPKHPYNQALQKAIPALHEKGEQLYTIPGAP
                     PDLSKPIPGCPFAPRCEFAQEKCVTSTIALKEVAPDHLSACLRIQLKEIDLTPASQPE
                     KLSRTM"
     gene            complement(3242..4144)
                     /locus_tag="VE486_01920"
     CDS             complement(3242..4144)
                     /locus_tag="VE486_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZA37875.1"
                     /translation="MNAVGETIGTEQALEEAERGTSLWRDAWHRLGKNKMAVISGCIL
                     ILITLAAFIGPLFLMQSYETQNLQLGAIPPGARHWLGTDTLGRDLFVRILYGGRVSMS
                     VGFCATAVALVIGVLYGAISGFLGGKIDILMMRVVDIIFALPFTVFVILLMVFFGRKF
                     VLLFAAIGAVQWLTMARIVRGQVLALRHQEFIEAAEALGLSKPRIIVRHMIPNTLGPI
                     IVYATLTVPAVMLLEAFLSFLGLGVQPPMSSWGVLIKEGAEVMEEFPWLLIYPGLILA
                     ITLFSLNFLGDGLRDALDPRASKD"
     gene            complement(4141..5058)
                     /locus_tag="VE486_01925"
     CDS             complement(4141..5058)
                     /locus_tag="VE486_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZA37876.1"
                     /translation="MLRFIARRLLQMVPVLFVIATLTFFMIRLAPGGPFDAERNTTPE
                     IQKSLDRYYGFNLPVYQQYFRWLGNALHGDLGPSFRYANRTVNELIGESFPISAELAF
                     WSLLIALALGLTAGVIASLRPNSPTDYLTSSFAMVGISIPNFVLGPLLVLVFALKLNW
                     LHVSGWEYPSDRILPALTLGALYAAYIARLTRGGMLEILSQDFIRTARAKGASNVRVI
                     LKHALRGGLLPVVNYLGPAAAALVTGSFAVETIFQIPGLGRYFVNAAFNRDYTLVLGA
                     VLFYASFIVVFNLIVDVVLVWMDPKQKFA"
     gene            complement(5140..>5333)
                     /locus_tag="VE486_01930"
     CDS             complement(5140..>5333)
                     /locus_tag="VE486_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37877.1"
                     /translation="LIVSVGAILGAFLWKLGPALNFLGAAALGVAGTIFYVKTVRQNR
                     QSFLDDLKKELAMRQRAPK"
BASE COUNT         1269 a         1452 c         1468 g         1144 t
ORIGIN      
        1 gatttgtgga cgttcggcgt gaatttttcc attgcgaatt cggacttgat cgacgcggtc
       61 catcaactca gagatgagat aaaaatgaaa actcgtgctc ccaaatgtcg cgatctgttg
      121 ctcgggcgcg agaatcacct gggtgttttc aattgcgtaa tcgaaatcgt ccttcttcat
      181 cgatctgata atttaagata gatgccgtct atcgcaaagc agaccggtac gggcgattta
      241 cgcaatcgcc caggcggttc taattaattt ccgggacgcg aatgcaggcg gcgaaatgtt
      301 tttctcccca atcaaccagc ggcggcacct gggctttgca ggcttcgatc atgtatgggc
      361 accgcggatg aaatcggcag ccgaccggcg ggttcatcgg cgaaggcgga tcacccggaa
      421 ggacaatgcg cttgcgcgca tgctcgcgtg ttggatcgac aatcggcacc gcgctgatga
      481 gggcctttgt atagggatgc aacggctttt ctaaaaccgc gcgagcttcg cccagttcaa
      541 caatcttgcc gagatacatc acggcaatcc ggtcggatat gtgtttcacg accgagagat
      601 catgcgagat gaagaggagc gtcagcctca tctcgtgccg gagttgggcg agtaaattga
      661 tgatctgcgc ctggatggag acatcgagcg cggaaaccgg ctcgtccgca ataatcaatt
      721 ttggttctac tgcgagggcg cgcgcgatcg cgatccgctg ccgctgtcca ccggaaaatt
      781 cgtgtggata ttttctcatc gtgcgcggca gcaaaccgac tttgcgcatt agcgcggcga
      841 cccgctgccg cagttcgcgg cgcgggactt tcttatgagt gcgcatcgct tccgccagcg
      901 tatcgaaaac gttcatccgc ggattgagcg acgcgtatgg atcctggaaa atgatttgaa
      961 aatcgctgcg cgcccggcgt agctcccgac cgcggagttg attcaaattg cgtccctcta
     1021 aaacgaccgt cccggaggtc ggcggaatca actgaacgat cgtgcgacca agcgtggatt
     1081 tgccgcagcc agattcgccc accaccccga gcacttcgcc ttcgttgatg gacagggaga
     1141 cgctgtcgac ggcccgcacc attccaagac ggcgcttaaa aatcagcccg gcttcgacag
     1201 ggaaatgcgt ctgcaggtcc tggatctcaa gaaacgcggg cggcatttgg cgagagacag
     1261 atgtactggc ggctgtccct ggtcgcaatt cttttgtcgg tgcgtgaagc cgcgcctggt
     1321 cgatccgcta ttgctgttgc cgctgctgct ctccgcccgg ctgggactga aaaagcgaca
     1381 gatattggcc gtaaccttct tcttttaatt tctcggcggg aataaaacgc aaggcagcgg
     1441 agttgagaca gtagcgttcg ccagtgggtg gcggaccgtc atcgaagaca tgaccgaggt
     1501 gcgcgtcgct cttggctgaa cggacctcgg tgcgctccat tccatgggaa gaatccctct
     1561 tttccaccac tttatcttcc gaaattggct tggtgaaact gggccagccg gtgccgctgt
     1621 cgaatttatc cagcgagctg aaaagcggct cgccggtgat aatatcgacg tagagtccgg
     1681 gctgatgatt atcccaatag gcattttgaa atggggtttc cgtgccgcct tcgcgcgtca
     1741 cgcgatactg gtctttgctt agatgccttc gcaattcggc atcactagga atttccttca
     1801 tcggattcat tttctcggct tgttttttct gcccttgagc atagccaaac aaaattgagc
     1861 cgacaaccac aacaacagcg gaaattaaag caatccacag agtcttatgc gaaccgtgct
     1921 tatactgtaa gtttctgccg tacattctct atctccagcg gctggatatt tagactaatc
     1981 cccgcgctct gccgttcaca ttgttcggga aagcttctcg ggctgagagg caggcgtcag
     2041 atcaatctcc tttaattgga ttcgtaaaca agccgacaaa tggtcgggcg cgacttcttt
     2101 caaggcgatt gtcgatgtca cgcatttttc ctgcgcaaat tcgcagcgcg gagcgaacgg
     2161 acaacctgga atcggcttcg agagatcagg cggagcaccc ggaatcgtgt aaagctgctc
     2221 tcctttttcg tggagcgcag gaatcgcttt ttgcagcgcc tgattataag gatgcttcgg
     2281 ctcgtaaaaa atccgggcgg tggaggcgct ctccacaatt cgtcctgcat acatgacaag
     2341 aacacgatcg caaaaaccag aaacgatgcc gacgtcgtgc gtgatgaaaa tcacagcggt
     2401 gccgagctcg ctttgcatct tgcgaatcag ttcgagaatt tgcgcctgca ccgtaacgtc
     2461 gagcgcagtc gtgggttcat cggcgatcaa gaccgccggc ttggtgatca aagctgccgc
     2521 gatcatcacg cgctgacgca tgccgccgga aaactcatgt ggataggagc gcattgtccg
     2581 ctcgggctct ggaattccga cttctcgcag ggccgcgagg gcgcgttggc gggcagcggc
     2641 gcgcgaaagt ctctcgtgaa tgaggagcgg ctccattacc tgattcccga ttcgcatgta
     2701 agggttgagc gaactcatcg ggtcctgaaa aatcatcgag atgcgatgcc cgcgaatttt
     2761 tcgcaattgt tcatcgtcgc aatgaagcag gtcgattccg ttgaaatcgg cgcgaccgct
     2821 ctcgattcga ccaggaggcg aggggattag acgcaacagc gaaaggcacg cgacggattt
     2881 gcccgacccg gattcgccca cgatgccaag cgtttcgcca gggttcacag aaaatgaaac
     2941 gccatcgacc gcccgcacaa ttcccgcgcg cgtatgaaaa taggtccgta aattttcaac
     3001 ccgaagcagc tctgccacga agtgaacagt gatcagggat ctgtgatcag taaagcattt
     3061 caggggcgca tactgttcac tgatcaccgt tcaccgatta ctcgttcgca gggcgcggat
     3121 cgagcgcgtc acgcaatccg ttacattgtc atcccgagcg gagtcgaagg atctctcaat
     3181 tgtcctttga tctttcgaga gatgtttcgc tcggctcaac acgacacgca ttaccgtttt
     3241 cctaatcttt tgacgcgcgc ggatcgagcg cgtcacggag gccgtcgccg agaaaattca
     3301 gcgagaaaag agtgattgcg agaattaatc caggatagat cagcagccac ggaaattctt
     3361 ccatcacttc agcgccttct ttgatgagca cgccccacga gctcatcgga ggttgcacgc
     3421 cgaggccgag gaagcttagg aacgcttcga gcagcatcac tgcaggaacc gtgagcgtgg
     3481 cgtaaacgat gattggccca agcgtgtttg gaatcatgtg ccgaacgatg atccgcggct
     3541 tggaaagtcc cagcgcttcc gcggcttcaa taaactcctg atgccgcagc gccagcacct
     3601 ggccacgaac aattcgggcc atcgtcagcc attgcaccgc accgattgcg gcgaaaagga
     3661 gcacaaattt gcgaccgaag aaaaccatca gcaggatgac aaacaccgta aaaggcagcg
     3721 cgaaaatgat atcgacgacg cgcatcatca gtatgtcgat cttgccgcca aggaatcccg
     3781 agattgctcc ataaagaacg ccgatgacca gggcgactgc ggttgcacaa aaaccgaccg
     3841 acatcgaaac ccggccgcca tacaaaattc tcacaaacaa atcccgcccg agcgtgtctg
     3901 tcccaagcca atgacgcgca ccaggtggta tcgcgccgag ctgaagattt tgtgtctcgt
     3961 aggattgcat cagaaaaagc gggccgatga atgccgcgag cgtaatcagg ataaggatgc
     4021 agccgctgat aactgccatt ttgtttttcc caaggcgatg ccacgcgtcg cgccagaggg
     4081 aggttccgcg ctcggcttct tcgagcgcct gctcggtccc gattgtttcc ccgacagcgt
     4141 tcatgcaaat ttctgtttcg ggtccatcca aacgagcaca acatcgacaa tcaaattaaa
     4201 gacgacgatg aagcttgcgt agaagagcac ggcgcccagc accagcgtgt aatcgcgatt
     4261 gaacgctgca ttcacgaaat accgtccgag tcccggaatt tggaaaattg tttcgactgc
     4321 aaacgatccc gtgacgagcg cggctgctgc cggccccagg taattcacca cgggaagcaa
     4381 tccgccccgc agcgcatgct ttaggatgac gcgcacattg gaggcgcctt tcgcgcgagc
     4441 cgtgcggata aaatcctgcg acagaatttc gagcatcccg ccgcgagtca gccgcgcaat
     4501 gtaagcggca tataaagctc ccaacgtgag cgccggcaga attcgatcgc ttggatattc
     4561 ccaaccggaa acatgcagcc aattcagctt gagggcgaag acgagaacga gaagcggccc
     4621 gagcacaaaa ttgggaatag agattcccac catggcaaac gagctcgtaa ggtagtcggt
     4681 cggactgttt ggccggaggg aagcaatcac gccggcggtg agtccgagcg ccaaagcaat
     4741 caataacgac caaaacgcca gctcggcgga aattggaaac gattctccaa tcaattcgtt
     4801 aaccgtgcgg tttgcgtaac ggaacgacgg ccccagatcg ccatggagtg cgttgccgag
     4861 ccaacggaaa tactgctgat aaaccggcag attaaaaccg tagtagcgat cgaggctctt
     4921 ctggatttca ggcgtcgtgt ttctttccgc atcgaacggc ccgcccggcg cgagacgaat
     4981 catgaagaag gtcagcgttg cgatgacgaa cagcaccggt accatttgga ggaggcgacg
     5041 tgcgatgaag cggagcatgt cttcgttaaa tcgttacaaa agttacaatg ttacatggga
     5101 gaactttgta acagttgtaa ccatgtaact ttgccgctgt catttcgggg ccctttggcg
     5161 catcgccaac tcttttttta gatcgtccag gaatgattgc cggttctggc ggaccgtttt
     5221 cacatagaaa atcgttccgg cgactccgag agctgccgca ccgagaaaat taagcgccgg
     5281 tccaagcttc caaaggaatg cgccgaggat cgcgccgacg ctcacaatca gat
//
LOCUS       DASQUQ010000068         9339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_921127, whole genome shotgun sequence.
ACCESSION   DASQUQ010000068 DASQUQ010000000
VERSION     DASQUQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921127"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VE486_01935"
     CDS             <1..1318
                     /locus_tag="VE486_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZA37878.1"
                     /translation="NGDGGDFAHYLLGPDGTWKQITQFSDQVKAARLGRDKALYLLSR
                     ADAARGKILRLPLDTPELKNAVEIVPAGQAVIQRIVPAADALYVGDLLGGPSHIRRFG
                     LDGKGEAIVPVLKISAVQEMLALEDNSLLFRDVSYTEPAAWFHCAQGKTEPVKTALRS
                     TSPVSFADIEVRREFATSKDGARIPLNIVFRKGMKTDGQNPTLLYGYGGYGVSVAPNF
                     DFTRRLWFDRGGVYVVANIRGGGEFGEEWHKAGNLTKKQNVFDDFAAAAEYLIKEKYT
                     RPEKLAIQGGSNGGLLMGAMITQHPDLMRAVVSSVGIYDMLRVELAPNGAFNVTEFGT
                     VKDPEQFKALYAYSPYHHVVDETKYPAILMMTGANDGRVAPYHSRKMTARLEEANKSG
                     NPILLRTSSSAGHGQGTALSERIKQSADIYAFLFAQLGMSRMTKSE"
     gene            1402..2742
                     /locus_tag="VE486_01940"
     CDS             1402..2742
                     /locus_tag="VE486_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37879.1"
                     /translation="MRKKLLIYGGAIAIFLLIAAAGGVAIFYSPLFTHYIEGNAFRAA
                     METETAKGLHFPRSRYAPIRRTSAFTAQSESFEASNGEKAMKSLTAHNIIARFNPWGV
                     FVRQWRFNDVHVQSGDVAIQIYEANPEEEKPKPWFAIFLPNRVYVERIESEQANVTWR
                     LRGERAGFFGTRLLITPNGWDFEYVATGGKLKMALLPDLELRRAHLLITKRLLTIYDV
                     DLASDAGSDESIHAQANAGIGKDKSVDFKANFNAVPIRSWLPAEWKGRLNGSAFGDLH
                     WAGKDPKLESSSGEGSLRIRNGRIDDVPLLEKLAELSRKKAFERLELNDCSLTFAWHY
                     PQIDIKDIMIEEKGKFRIEGEITVERRRLRGTIRLGLTREYLDWLPNPEEVFSRHESG
                     YLWTTVHLSGTIDEPAQDLSPRIIELFKESPGAYLGLLFRQFEGWLKKAFGGVH"
     gene            complement(2736..3977)
                     /locus_tag="VE486_01945"
     CDS             complement(2736..3977)
                     /locus_tag="VE486_01945"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZA37880.1"
                     /translation="MKAGFLEKLIERLGRIGPEEVQNYFLRLAQEKGFLETVFNAIQE
                     GIIVTDSKGHITYLNDAACELFGLRAIDAIGKRLDERVRGLDWESLTQSSGPVSRDME
                     IFYPANRFINFYIVPLMIEHRETGAVTGVADSRHRDGPGYSEQVGHAMILRDITESRR
                     SAQQTIESERLNALTLLAAGVAHEIGNPLNSLHIHLQLMERQAHRLDRAAREELQESI
                     DIARSEVNRLDSIVTQFLRAIRPSKPELHPEGVNTIVEETLRFLTPEIQDRDIAIEQE
                     LRSDLPMLQIDRDQMKQAFYNVIKNSLEAMKRGGTLRIHTDRDDTHVLISFVDTGSGM
                     SAENLGRVFEPYFTTKPSGSGLGLLIVRRIVREHGGELSIESSEGKGLTLTIRLPYID
                     KRVRMLEAGEARNERPEESGQ"
     gene            complement(4102..5112)
                     /locus_tag="VE486_01950"
     CDS             complement(4102..5112)
                     /locus_tag="VE486_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZA37881.1"
                     /translation="MSTSTRVSYGVLAFTIILAGFLHLGGPLLAAFFSFLALRKLRLL
                     TKRKWLASILFGLVVAGLAFGAVYFTRAAVHALPEVAEHSIPSASAWAQARQIELPFT
                     DVDTLRDFLINSVKEQARYLQNVAHFAGRTTTYLLFTIIAIVAASSLFLKSGFNPYEE
                     THAVKNNLYSVYSDEVSARFGDFYHSFSTVMGAQIMISSINTVLTGVFVLAVQLPYAP
                     LAIAITFLCGLVPIVGNLVSNTIIVFLALTVSLKLAIGALVFLVLIHKLEYFLNSKII
                     GSRIRNPVWLTLIALIIGERLMGIPGLILAPVVLNYLRVEMLKIEVPPAPERSIVEPL
                     NR"
     gene            complement(5211..7361)
                     /gene="pnp"
                     /locus_tag="VE486_01955"
     CDS             complement(5211..7361)
                     /gene="pnp"
                     /locus_tag="VE486_01955"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZA37882.1"
                     /translation="MQQKVTAQIGANQISIETGRMARLADGAVVVACGDTMVLASAVS
                     ATTIKEGQDYFPLTVDYREKAAAVGKFPGGYFKREGRPTEKETLTSRMIDRPLRPLFP
                     QGYFYDTQIISILLSADGENDPDILALNGASAALCVSDIPFAGPIGAVRVGRVNGQFV
                     ANPTHAEREQSDLDLVYVGTENDVIMIEGSANELPEAEFVKALEFAHGQAREMIRIQK
                     ELAASVGKSKRDVPLLQVKQDLLDIAYRVAGDRIEAALYTEGKVARAKAVDALREEVK
                     TSILEKYPEADPFSISQAFDYVQKKAFRISILDKQKRVDGRGYQDLRQISCEVGVLPR
                     AHGSAIFQRGETQALTLATLAPIEEAQMLDAYGGGEQSKRFILHYNFPPFSVGETGRT
                     GGPGRREIGHGALAERSLEPVVPSENDFRYAIRISSEIMESNGSTSMASVCGGMLALM
                     DAGVPVKTPIAGISVGLVTEFGDDRQLKRYELLTDIIGSEDHFGDMDFKLCGTDAGVT
                     GFQLDLKLPGVSHKIMAEAIQRAKEARTKILGIMRGALDKPRPELSKYAPRIETIKIN
                     PEKIGALIGPGGKTIKGIVAETGAEINIEDDGSVHIYATSGESMARAKEIIGGMTREI
                     EIGQTYQGRVVSTKEFGAFVEVFPGRDGLVHISELADFRVKRTEDVAKVGDIIWVKCI
                     GIDDKGRVKLSRKAALKERAEAETGQPAPAEHEH"
     gene            complement(7556..7819)
                     /gene="rpsO"
                     /locus_tag="VE486_01960"
     CDS             complement(7556..7819)
                     /gene="rpsO"
                     /locus_tag="VE486_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZA37883.1"
                     /translation="MAESTETLSLKEFQLHEKDTGSADVQVALLTRRIEHLTEHLKTN
                     RKDHSSRRGLLKMVAQRRSLLDYLSKTKADRYKQLIDKLNLRK"
     gene            complement(8004..8510)
                     /locus_tag="VE486_01965"
     CDS             complement(8004..8510)
                     /locus_tag="VE486_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37884.1"
                     /translation="MKKLISLLVICSVCGFTAAFAANKANLNQAIASLDARAQTNADK
                     KLVLKAVSQQTNLPVKTLQSQMNATHLSYGELLTADSIVEGSGKNLNTVLAMRKGKDW
                     ASLSKELRVDPDSIVSRLRSAEDTVRATQAATRAQNTQRAQGFKRGSSYGPQAGGPMG
                     NTNNRGPR"
     gene            complement(8606..>9339)
                     /locus_tag="VE486_01970"
     CDS             complement(8606..>9339)
                     /locus_tag="VE486_01970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA37885.1"
                     /translation="GRSQAQEEMISFIIPAYNEELELSSTIGAIRAAASGAAQPYEII
                     VVDDASIDATAKIATKAGARVISINRRQIAAARNAGARSAQGEYLLFVDADTRINQAH
                     IAEPIAALEAGYAGGSARVAMNGVIPLWGRVFVRLFCTVYFGLNLGAGAFLFTTRRNF
                     DAIGGFDEQYFVGEEVYFSLALRKLGRFKVLREPILTSGRKLRMYSARQVLTRSFSII
                     IGGPSSARSRDKLDVWYNGKRETGS"
BASE COUNT         2066 a         2563 c         2593 g         2117 t
ORIGIN      
        1 gaatggcgat ggcggcgatt tcgcccatta tttgctcggc ccggacggga cgtggaagca
       61 gatcacgcaa ttcagcgatc aggtcaaagc tgcgcgcctg ggacgcgaca aggcactcta
      121 tcttctttca cgcgcggatg cggcgcgcgg caaaattctg cgcctgccgc tcgacacacc
      181 agaattaaag aacgcggttg agatcgttcc ggctggccaa gcggtcatcc agcgaatcgt
      241 tcccgctgcc gatgcgctct atgttggcga cttgctcggc ggcccgtctc atatccggcg
      301 ctttgggttg gacggcaaag gcgaagcaat cgtcccagtt ctaaaaatat cggctgttca
      361 ggagatgctc gccctggaag acaactcgct cctgtttcga gatgtgagtt acaccgagcc
      421 ggcggcatgg ttccattgcg cccagggaaa aaccgagcca gtgaagacgg ccttgcgcag
      481 cacatcaccg gtctcatttg ccgacatcga agtgaggcgc gagttcgcaa cttcgaagga
      541 tggcgccagg attccgctca acatcgtttt ccggaaagga atgaagacag acgggcaaaa
      601 tccgacgctg ctttacggct atggcggcta tggagtcagc gtggcgccta atttcgattt
      661 tacgcggcgg ctctggttcg atcgcggcgg cgtgtatgtc gtggcaaaca ttcgaggcgg
      721 cggagaattc ggcgaggagt ggcacaaggc cggcaatctc accaaaaaac aaaacgtgtt
      781 cgatgatttc gccgccgctg ccgaatattt gatcaaagag aagtacacgc gcccggaaaa
      841 actcgcgatc cagggcggaa gcaacggcgg tctcttgatg ggcgcgatga ttacgcaaca
      901 tcccgatttg atgcgggcgg tcgtttcctc agtcgggatt tacgacatgc tgcgggtcga
      961 gctcgcgccg aatggcgcct tcaatgtgac cgaattcggc acggtaaaag atccggagca
     1021 attcaaagcg ctttacgcct actcgcccta tcatcacgtt gttgatgaaa cgaaatatcc
     1081 cgcgatcctg atgatgaccg gcgctaacga cgggcgggtc gcgccctatc actcgcggaa
     1141 aatgacagcg cgcctcgaag aagcgaacaa atccggcaat ccgattctat tgcggaccag
     1201 ctcgagcgcg ggccatggcc agggcaccgc cttgagcgag cgcatcaagc aatcggcgga
     1261 tatttacgcg tttctttttg cgcaattggg aatgagcaga atgacgaaat ccgaatgaca
     1321 agaagccacg ctttgagctt cgccattcct cattgattcg gatttcgtca ttcgtcattt
     1381 gtcataattc agttcccgac cttgcgaaag aaattgctaa tttacggcgg agcgattgcg
     1441 atttttcttt tgatcgcagc ggcgggtggc gtggccattt tctacagtcc gctgttcact
     1501 cattacatcg agggcaacgc ctttcgcgca gccatggaaa ctgaaaccgc gaaaggtttg
     1561 catttccctc gtagccgcta cgcgccgatt cggcgcacca gcgcattcac cgcgcaaagc
     1621 gaaagtttcg aagcgagtaa tggcgagaaa gcgatgaagt cactcactgc tcacaacatc
     1681 atcgccaggt tcaatccatg gggcgtattt gtgcggcaat ggaggttcaa cgacgtgcac
     1741 gttcaatccg gcgacgtcgc tattcagatt tacgaggcaa atcctgaaga agagaagcca
     1801 aagccatggt tcgcaatttt tttaccgaac agagtttacg tggagaggat tgaatcggag
     1861 caggccaacg tgacctggcg attgcgcggc gagcgggccg gcttttttgg cactcggctg
     1921 ctcattacgc cgaacggctg ggatttcgag tatgtcgcca ccggcgggaa actgaagatg
     1981 gctttgcttc ctgatcttga gctgcgccgc gcgcatcttc tcatcaccaa gaggctgctg
     2041 acgatttacg atgtcgatct cgcatcggac gcaggtagcg acgaaagcat tcatgcccag
     2101 gcgaacgctg gcatcgggaa ggataagagc gtcgatttca aggcgaactt caatgcggtc
     2161 ccaattcgtt cgtggttgcc agcggaatgg aaagggcgtt tgaacggcag cgctttcggc
     2221 gatcttcact gggcgggaaa ggatccgaag ctggaaagct catccggcga agggtcgttg
     2281 cgcatccgta acgggcgaat cgacgatgtt cctcttctgg aaaagctcgc ggagctctcc
     2341 cggaagaagg cattcgaacg tttggagctg aacgattgtt cgctcacttt cgcgtggcac
     2401 tatccgcaaa tcgacatcaa ggacatcatg atcgaagaga aaggcaagtt ccggatcgag
     2461 ggcgagatca ctgttgagcg gcgtcgtttg cgcggcacga ttaggcttgg gcttacccgt
     2521 gaatatctgg attggttgcc gaatcctgaa gaagtcttta gccggcacga gagtggttat
     2581 ctatggacca ctgtgcatct gtccgggaca attgatgaac ccgcccaaga cttgagtccg
     2641 cgcattatcg aacttttcaa ggaatcgccc ggcgcgtatc tcggtcttct ctttcgtcag
     2701 ttcgaaggct ggttgaaaaa agcattcggc ggcgttcact gaccactctc ctccggcctc
     2761 tcattcctgg cttcgcccgc ttccagcatc cgaacgcgct tgtcgatata tggcaatcga
     2821 atggtgagag tgagaccttt gccttcgctg ctttcgatgg aaagttctcc gccgtgctcg
     2881 cgcacaattc ggcgaacgat cagcaaccca agaccggagc ctgacggttt ggtcgtgaaa
     2941 taaggctcaa aaacgcggcc caaattctcg gccgacatac cgctgcctgt atcgacgaaa
     3001 ctgataagga cgtgggtgtc gtctctatcg gtgtggatgc gcagcgtgcc accgcgcttc
     3061 atggcttcaa ggctgttctt gattacgttg taaaaagctt gcttcatctg atcacgatcg
     3121 atctgaagca tggggagatc cgaacgcagt tcctgttcga tggcaatgtc acggtcctga
     3181 atttcggggg taagaaaacg cagtgtttcc tcgacgatcg tattcacgcc ttccgggtgc
     3241 agttctggtt tggaaggtcg aatcgcgcgc aaaaactgag tcacaatcga atcgagccgg
     3301 ttgacttccg aacgggcgat gtcgatcgat tcctggagtt cctcccgcgc ggcgcgatcc
     3361 aatctatgcg cctgccgctc catcaactgc aaatggatgt gcagcgagtt gagcggattg
     3421 ccgatctcgt gcgcaacgcc cgcggcgagc agggtaagcg cattcaatcg ttccgactca
     3481 atagtctgct gcgcagagcg ccggctctcg gtgatatcgc gcaggatcat ggcgtggcca
     3541 acctgttcgc tgtagccggg accatctcga tgtctggagt cagcgactcc agtcaccgcc
     3601 cccgtctcgc ggtgttcgat catcaagggc acgatgtaaa aattaatgaa acgatttgcc
     3661 ggataaaaaa tttccatgtc gcgactgact ggcccactgc tctgagtaag agattcccaa
     3721 tcgagaccgc ggacgcgctc gtcgagccgt tttccgatgg cgtcgatggc gcgaagccca
     3781 aacaactcgc acgccgcatc attaagataa gtgatgtggc cttttgagtc ggtcacgatg
     3841 atgccttcct ggatggcatt gaaaacggtc tcgaggaaac ctttctcctg cgccaggcgc
     3901 aaaaaataat tctgcacctc ttccggtccg atgcgtccga ggcgctcgat taatttttcg
     3961 agaaaacccg ctttcacggt gtgattacat ctcctttaaa atgggagatt ccttgactcc
     4021 gctcggaatg acaaaaggaa accagctttc acggcgagcg ttagatcgtt accccgttaa
     4081 atcgttacaa cgcaagatgc ttcaccgatt taacggttca acgattgacc tttccggcgc
     4141 cggtgggact tctattttca gcatttccac ccgcagataa ttcagtacca ccggcgcaag
     4201 aataaggccg gggataccca tgagtctttc gccgatgatc agcgcgatca acgtcagcca
     4261 gacggggttg cgaatccggc tgccgatgat tttgctattt aagaaatatt cgagcttgtg
     4321 aatcaaaaca agaaacacca gagccccaat ggccagcttc aacgaaaccg ttaacgcaag
     4381 gaaaacaatg atcgtattgc tcaccaggtt gcccacgatg ggcacgagcc cgcagagaaa
     4441 tgtgatcgcg atggcgagcg gcgcatacgg caattgaacc gccaggacga aaacgccggt
     4501 cagaaccgta ttgatcgacg agatcataat ctgtgcgccc atgacggtgg aaaagctgtg
     4561 gtagaaatcg ccgaatcggg ccgagacctc atcgctgtaa acggagtaaa gattgttctt
     4621 aactgcatgc gtctcctcat agggattgaa gccgctcttt aaaaacagac tcgaggcagc
     4681 gacaatcgct atgatcgtga agagcaggta ggtggtcgtc ctgccggcga agtgggccac
     4741 gttttgcagg taacgcgcct gctccttcac ggaattgatt aagaaatccc tgagagtgtc
     4801 gacatctgtg aagggcaatt caatctgccg cgcctgggcc cacgcgctgg cagatggaat
     4861 cgaatgctcg gccacctccg gcagcgcgtg gacagccgcc cgggtaaaat aaacggcgcc
     4921 aaaggcgagt ccggccacca ccaacccgaa caggatcgag gcgagccatt ttcgttttgt
     4981 tagcaagcgg agtttgcgca atgcgagaaa ggagaaaaac gcggccagca aaggccctcc
     5041 aagatgcagg aaccctgcga gaataattgt gaaggcgagc acgccgtagg agactcgggt
     5101 ggatgtgctc atggatcagc gtttcggcat ccgtttaact tcacccgcag ccgtgcttgt
     5161 caacctcact caaatcgacc gggatcaccg gcactgacgg caagctctga tcagtgttcg
     5221 tgttctgccg gagcgggctg acccgtttcg gcttcggcgc gctctttcaa cgcggctttg
     5281 cgagaaagtt ttacgcgccc tttgtcatcg atcccgatgc acttgaccca aatgatgtcg
     5341 ccaaccttgg caacgtcttc ggttcgtttc acacgaaaat cagcgagctc ggaaatgtgc
     5401 acgagcccat ccctgcccgg gaatacctcg acaaacgcgc cgaactcctt cgtcgagaca
     5461 acgcgaccct gatacgtctg gccgatctcg atctcacggg tcatgccgcc gataatttct
     5521 ttcgcgcgcg ccatgctttc gccgctcgtc gcgtagatgt gcaccgagcc gtcgtcctcg
     5581 atgttaatct cagcgccggt ctccgcgaca atgcccttga tggtcttgcc gccgggtcca
     5641 atcagcgcgc caatcttttc cggattgatc ttaattgttt caatacgcgg cgcgtatttc
     5701 gataattcgg gccgcggctt gtccagcgcg ccgcgcatga tgccgagaat tttcgtgcgc
     5761 gcttccttcg cgcgttggat cgcttcggcc atgatcttgt gactgacccc tggaagtttc
     5821 agatcgagct gaaaaccagt gacgccagcg tccgttccgc aaagtttgaa atccatgtcg
     5881 ccgaaatgat cttccgagcc aatgatgtcc gttagcaatt catagcgctt caattgccgg
     5941 tcatcgccaa actcggtaac caggccgacc gaaattccgg cgatcggcgt tttcaccggc
     6001 acgcccgcgt ccatcaacgc cagcataccg ccgcacacgc tggccattga tgtcgatcca
     6061 ttcgattcca tgatttcgct tgatatgcgg atggcgtaac gaaaatcgtt ttcgctcggg
     6121 acaaccggtt cgagggaacg ctcggccagc gcaccgtgac caatctcgcg tcgcccaggc
     6181 ccgcctgttc gtcccgtttc gcccacgctg aagggtggaa aattgtagtg caaaatgaag
     6241 cgcttcgatt gctcaccgcc gccgtaagca tcgagcatct gcgcttcttc gatgggtgcg
     6301 agtgtggcga gcgtgagcgc ctgtgtttcg ccgcgctgga aaattgcgga cccatgcgcg
     6361 cgcggcagga cgccgacttc gcacgaaatt tgacgcagat cctgatagcc gcggccatcc
     6421 acccgttttt gtttgtccag aatgctgatg cgaaatgcct tcttctggac gtaatcgaat
     6481 gcctgcgata tcgaaaaagg atcggcctct ggatattttt ccaggatcga agtcttcact
     6541 tcctcgcgca atgcatcgac cgctttggcg cgtgcgacct tgccttcggt gtaaagcgcc
     6601 gcttcaatcc ggtcgcccgc gacgcggtaa gcgatatcga gcagatcctg cttgacctgc
     6661 aacaacggca cgtcgcgctt cgatttgcca acgctcgcgg ccagttcttt ttgaatgcga
     6721 atcatctcgc gcgcctgtcc atgcgcgaac tctagcgctt tcacaaactc ggcttcgggc
     6781 agctcgttcg ccgagccttc gatcatgatt acgtcgtttt cggtgccgac ataaacaaga
     6841 tcgagatcgc tctgttcacg ttcggcgtgc gttgggttcg caacgaactg gccattcact
     6901 cgaccaaccc ggacggcgcc gattgggccg gcgaatggaa tgtccgacac gcaaagagcg
     6961 gcggaggccc cattgagcgc cagaatgtcg ggatcatttt cgccgtcggc gctgagcagg
     7021 atcgagatga tctgggtgtc ataaaagtat ccttgtggaa agagcggccg aagcggccga
     7081 tcgatcatgc gcgaagtaag cgtttccttt tccgtcggtc gcccttcacg cttgaaatag
     7141 ccgccgggaa atttccctac ggctgcggcc ttttcgcgat aatcgactgt gagcggaaaa
     7201 taatcctgac cttctttgat tgttgtagcc gatacggcgc tggcgagcac catggtgtcg
     7261 ccgcaggcga cgaccactgc gccatcggcg agtctggcca ttctgccggt ttcgatagag
     7321 atttgattgg ccccgatttg ggccgtgacc ttctgttgca ttgtactttt cctggatgtg
     7381 cgagttccag gccggtcacc ctgactgtag gttttaaccc cgcgcccacg ggactaaaac
     7441 ctaaaatcca aggttactgc ggcctggact gctggtttgt gttgtgtttt ctaatctact
     7501 tctggcttcg agctgccaaa ccgttggacg cgaccttccc gaaaaatcgg ggcgattact
     7561 tgcgcaggtt cagcttgtcg atcagctgtt tataccgatc ggctttcgtc ttgctcaaat
     7621 aatcgagcaa actccggcgc tgtgcgacca tcttaaggag gccgcgacgc gaggagtgat
     7681 ctttgcggtt ggtcttgagg tgttcggtca aatgctcgat ccggcgggtg agaagtgcga
     7741 cctgcacatc cgcactgccg gtatcttttt catgcagttg gaactccttg aggctcaacg
     7801 tttccgtgct ctctgccata acgaagtgcc tattctaaat gaagaatgtg ctttcgcaac
     7861 tgcaggttat gaacctctgc aggatgccac gcttaaaaga gtggcgaaat ccagtcgggc
     7921 gtgggcttaa aggataagtt tacacccctc gcgtgcgagg gcagtcatgt cgatcgtaaa
     7981 cgattgaact gaaggaaaac cgtttaccga gggccacggt tattcgtatt tcccataggg
     8041 ccaccggctt gcggaccgta ggaggatccc cgtttaaaac cttgagcgcg ctgagtgttc
     8101 tgagcacgtg tcgcagcctg agtggcccgc acggtgtcct ctgcgctgcg aaggcgactc
     8161 acaatactgt cgggatccac cctgagttct ttcgagaggc tggcccagtc tttccctttt
     8221 ctcatggcga gcactgtatt caagttcttg ccactgcctt cgacgatcga gtcggccgtg
     8281 agcaactcac cataactgag atgtgttgcg ttcatttgcg attgcagcgt ttttactggc
     8341 aagttggttt gctgcgagac ggccttcaac acgagttttt tatctgcatt ggtctgggct
     8401 cgggcgtcca gggacgcaat tgcttgattc aaattcgctt tatttgcagc aaacgcggcc
     8461 gtgaagccgc aaaccgagca gatgacgagc agggagatta actttttcat gatttagatt
     8521 atgccattgc ggacgcgcta cgaaaagcta aaacgagcgg cagcagccga cagcaaagct
     8581 ttcctggcgg aatgcttcga cggagtcacg atccagtctc gcgtttgccg ttgtaccaaa
     8641 catcaagttt gtcacgtgag cgtgcggagc tcggcccgcc gatgataatc gaaaacgaac
     8701 gggtgagaac ttgccgtgcg gagtacatgc gcaatttccg gcctgacgtc agaatcggtt
     8761 cccgcagcac tttgaagcgg cctaattttc ggagggcaag gctgaaatag acttcttcgc
     8821 cgacgaaata ttgttcatcg aagccaccaa tcgcatcgaa attgcgccgg gttgtgaaaa
     8881 gaaacgcgcc cgccccgaga ttgaggccaa aataaacagt gcaaaaaagg cgcacaaaaa
     8941 cgcgccccca cagcggaatg actccattca tcgctacccg tgcgctgcca cccgcataac
     9001 cggcttcgag agcggcgatg ggttcggcga tatgcgcctg gttgatgcgg gtatcggcat
     9061 ccacgaacaa aagatattcg ccttgtgcgg accgcgcccc ggcgttacgc gccgccgcga
     9121 tttggcgtcg attgatcgat atcacgcggg cgcctgcctt cgtggcaatt ttggctgtag
     9181 catcaatcga tgcatcatcg acgacaatga tttcatacgg ctgggccgcg ccggatgccg
     9241 cagcacgaat ggcgccgatg gtcgaggaga gctccaactc ttcgttgtat gccggaatga
     9301 taaaggaaat catttcttcc tgggcttggg atcgaccgg
//
LOCUS       DASQUQ010000069         6345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_281601, whole genome shotgun sequence.
ACCESSION   DASQUQ010000069 DASQUQ010000000
VERSION     DASQUQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281601"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VE486_01975"
     CDS             complement(<1..323)
                     /locus_tag="VE486_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37886.1"
                     /translation="MPSSSLWQRFQQYFLYYRDLDFSLDISRMKFPDDFFEKMQPKIE
                     NAFAAMRELEAGAIANPTEKRMVGHYWLRNPALAPNAEIRAEIEATIKDIKSFAADVH
                     AGKIAA"
     gene            complement(365..2767)
                     /locus_tag="VE486_01980"
     CDS             complement(365..2767)
                     /locus_tag="VE486_01980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZA37887.1"
                     /translation="MTNVETMTNDKMTKPYRSCLSDFVLRHSFVIRHSCFVIFLALGV
                     GPLFAEKRAITEKDLFDFVWIGDPQVSPDGMRVAFVHVTVNEKKKGYNTSIWSVPVAG
                     GEEPHQLTKGDHDSTPRWSPDGKFLLFLRATEKEGKPEPPQLSILPMAGGDSFVFTDL
                     PKGASNPVWSPDGKMIAFTSETNADDLAKQEKKKRKEEELKKAVSASSPSGSVEPSKK
                     PAEKSEKNGGDKAAKKAESESEYESDVRVITRAVYREDNDGYADPKHPQHIWVVTAPH
                     TADEKVKPKQLTFGRFNEGSVVWSNDAAQIYFTSLRVDEPYYELPRTDLYSIRASGGE
                     PAKLNSIDMEIDNLSLSPDGKQVAFVASAAQPINSYTQPDLWIVDLTPNAKPRNLTAS
                     FDFDVGSGVFGDNAAPRAGGTNIPLWTPDGRSLIEVCGKEGKTILASFDTQTGAETDL
                     THGNQAVVRFRASADRSEIVYTLSTPTRINDLFVIDRSGPAVAARQLTRVNDALFSTL
                     NLTEPEEIRYKTFDGKTIQAWLQKPPDFNPAKKYPLILNIHGGPHSAYGYIFAHEFQW
                     MAAKGYVVLYPNPRGSTTYGQEFGNVIQYHYPGDDYKDLMAGVDDVVRRGYIDEKKLG
                     VTGGSGGGLLTNWVVGQTDRFAAAVSQRDIASWGDWWYSADFTLFQPNWFKAPPFEDE
                     QDFKARSPIDYIRNVKTPMMFILGEADYRTPPGAGGEQMFRALKFRKIPTVMVRFPDE
                     SHELSRSGQPWHRIERLQHIIGWFDHWLLGIRKPEYEVPAEEEVPTKKRNVPGKQPAP
                     PR"
     gene            complement(2807..4117)
                     /gene="icd"
                     /locus_tag="VE486_01985"
     CDS             complement(2807..4117)
                     /gene="icd"
                     /locus_tag="VE486_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZA37888.1"
                     /translation="MPYKDVAPPPGEKITRTDKLNVPDRPVIPFIRGDGTGPDIWAAS
                     VRVFDAAVEKAFGGRKKIAWFEVFAGQAAKDKFDEWLPDDTVHAFREFLVGIKGPLTT
                     PVGGGIRSLNVALRQLLDLFVCLRPVQYFKGVPSPVKHPEKVNMVIFRENTEDIYAGI
                     EYAAGTPEAQKILNFFQKEFPKEFDKIRFGTKEKAAEFWKRVGAPESDEVCVGIGIKP
                     VSRSGSVRLIHSAISYAIQNQRKSVTLVHKGNIMKFTEGAFRDWGYEIAKQYFGAEEI
                     EGGPWCKIPMGNPGAGIVIKDAIADITLQQVLTRPEDFDVIATLNLNGDYLSDALAAQ
                     VGGIGIAPGGNINYITGHAVFEATHGTAPKYANQDKVNPGSVILSGEMMFRYMGWTEA
                     ADLILKGLEGAIASKRVTYDFARLMEGATEIKCSEFGDNVIEHM"
     gene            complement(4325..5494)
                     /locus_tag="VE486_01990"
     CDS             complement(4325..5494)
                     /locus_tag="VE486_01990"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HZA37889.1"
                     /translation="MEWNIKEIMNIRPRAILTIPPLVFAFAICLPSAPAAEDLKEAKV
                     TQVIQDVKVLPSNAAPRPAVVNDNVRQGTGVQTGVQSRSELTFKDKTITRLGEKTIFN
                     VGEGARTIELSSGQFLLYVPKKSGGAKVKMGPVTAAITGTTVLGNVDPSGIASFIVLE
                     GSACIRLDSVGQSLQVHAGQKLTFDPVTNRLEDPVDVDLNQVLTSPLIKDFRRLPSAP
                     LIEQQIERQHGAQDGVSSNPDLTRAIRVAGASSPGTATPDQFMRALNSLFARSSSQEV
                     CGYASAAVRARPDLAGRIVTAALGASQSAGPESADCIIRGAIAAHPAATREIVSAAVA
                     AAPSLRDSIVAAANIAQPCQEANAFVQPAIPTTINAANFSSAVVSPEQPTQSSDE"
     gene            5791..>6345
                     /locus_tag="VE486_01995"
     CDS             5791..>6345
                     /locus_tag="VE486_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZA37890.1"
                     /translation="MKEPFLPLSRRLDYRSPIIRLRLATARRADYHPLLPRERASAFT
                     LLELLVVIGIIAILMVLLVPAFTSIKGGTDVTNAAYTIKGVLDTARTYAKANNTYTWV
                     GFYEEDVSQPSTNPPTPGTGRLVMSIVASKDGTTVYNPNSTLNPDPIDPTKLTQVNKL
                     VKIDNVHLPLFAVCTANCSGDTFDT"
BASE COUNT         1301 a         1854 c         1686 g         1504 t
ORIGIN      
        1 gcggcgattt ttcccgcgtg aacatccgct gcaaagcttt tgatgtcttt gatcgttgct
       61 tcgatctctg cgcgaatttc cgcgtttggg gcaagcgcgg ggttgcgcag ccagtaatga
      121 ccgaccatgc gtttctcggt gggatttgcg attgctcccg cctctagctc gcgcatggcc
      181 gcaaacgcgt tctcgatctt cggctgcatt ttctcgaaga aatcgtccgg aaatttcatc
      241 cggctgatgt cgagcgaaaa atcgagatcg cggtagtaca gaaaatattg ctgaaaacgc
      301 tgccagagcg aagaggaagg catcgactca gctaacctca acggacgatt gaggtcaatc
      361 gcccctacct tggtggcgcc ggttgtttgc ccggaacgtt acgctttttg gtcggcactt
      421 cttcttccgc tgggacttcg tattctggtt tgcgaattcc gagcagccag tggtcgaacc
      481 aaccgatgat gtgttgcagc cgctcgatgc gatgccacgg ctggccagag cgggaaagct
      541 cgtgagattc gtcggggaaa cgcaccatca ccgtgggaat tttgcgaaac ttgagcgcgc
      601 gaaacatttg ctcgccgcca gcgccgggag gggtacgata gtctgcttcg cccaggataa
      661 acatcatcgg cgtttttaca ttcctgatgt aatcgatcgg cgatcgcgcc ttgaaatcct
      721 gttcgtcctc gaacggcgga gccttgaacc agttcggctg aaacagggta aaatcggccg
      781 agtaccacca atctccccag ctcgcaatgt cacgctgaga aaccgccgcg gcaaaccggt
      841 cggtttgtcc gacaacccag ttcgtcagca atccgccgcc actcccgccg gttacaccga
      901 gttttttttc gtcgatgtag ccacggcgga ccacatcatc cacccctgcc attaaatcct
      961 tataatcatc gccgggataa tgatactgaa tgacgttgcc gaactcctgc ccgtaggtcg
     1021 tgctgcctcg cggatttgga taaagcacca cgtaaccttt cgctgccatc cactggaact
     1081 cgtgcgcgaa gatgtagccg taagccgagt gcggtccgcc gtgaatgttc agaatgagcg
     1141 gatacttttt cgcgggattg aagtccggcg gtttttgcag ccatgcctgg atagttttac
     1201 catcgaaagt tttgtaacga atctcttccg gctcggtgag attaagcgtt gagaaaagcg
     1261 cgtcattgac gcgggttaac tggcgtgccg cgactgcggg accgctgcga tcgatcacga
     1321 agagatcgtt tatccgcgtc ggcgtcgaca gcgtgtaaac aatttccgat cgatctgccg
     1381 acgcccggaa acgaaccacc gcctggttgc cgtgcgtcag gtcggtctct gcgccggttt
     1441 gggtatcgaa cgaggcgaga atcgttttac cttcttttcc gcacacctcg atcaaactgc
     1501 gtccgtcggg cgtccacagc gggatattcg tgccgccggc gcgcggagcc gcattgtcgc
     1561 cgaaaacgcc gctgccgaca tcgaaatcga aacttgcggt tagatttcgc ggcttggcat
     1621 ttggcgtgag atcgacaatc cacaagtccg gctgcgtgta ggaattgatc ggctgcgccg
     1681 ccgacgcgac aaacgcgacc tgtttgccat cagggctgag tgacaaatta tcaatctcca
     1741 tgtcgatcga attaagtttc gccggttcgc cgccgctggc gcggatcgaa tagagatcgg
     1801 tccgcggcag ttcgtaatac ggctcgtcca cacgcagcga cgtgaaatag atctgcgctg
     1861 catcattcga ccaaacgacg ctgccttcat tgaatcggcc gaacgtcagc tgtttcggct
     1921 tcactttttc atccgcagtg tgtggcgcag tcacgaccca gatatgctgc ggatgcttcg
     1981 ggtcggcata gccatcattg tcctcccgat agactgcgcg cgtgatcacg cgaacgtcgc
     2041 tttcatattc agactctgac tcggcttttt tggccgcttt gtctccgccg tttttctcgc
     2101 ttttttccgc cggtttcttc gaaggttcaa ccgaacctga tggcgagctt gcgctgaccg
     2161 ctttctttaa ttcttcttct ttgcgctttt tcttttcctg tttggcgaga tcgtctgcat
     2221 ttgtctcgct cgtaaatgcg atcattttcc cgtccggcga ccacactgga ttgctcgcgc
     2281 ccttcggcag atcggtaaaa acaaatgaat cgccgcccgc catcggcagg atcgacaact
     2341 gcggcggctc cggtttgccc tccttttccg tggcgcgcag gaagagcagg aattttccat
     2401 ccggcgacca gcgcggggtg gaatcgtgat cgccttttgt cagttgatgg ggttcctcgc
     2461 caccagcgac aggcacggac cagatggaag tgttatagcc ctttttcttc tcgttaactg
     2521 ttacgtgcac gaaagcgacg cgcatcccat ccggcgaaac ctgtggatcg ccgatccaga
     2581 caaaatcgaa caggtccttt tccgtaatgg cacgtttctc ggcgaaaagg ggaccgaccc
     2641 cgagagcgag aaaaatgacg aagcacgaat gacgaatgac gaaggaatga cgaagcacga
     2701 agtccgaaag gcaagatcga tacggttttg tcattttatc atttgtcatt gtttcgacat
     2761 tcgtcattcg gatttcgcct gccacgccga aggcgggtca ttcgcatcac atgtgttcga
     2821 tgacattatc cccaaactcg ctgcacttga tttcggtcgc gccttccatc aaacgggcga
     2881 aatcgtaggt gacgcgcttg ctcgcgatcg cgccctctaa tcccttgaga atcagatcgg
     2941 ctgcttccgt ccagcccatg taacggaaca tcatttcgcc cgaaagaatc accgaaccgg
     3001 gattgacctt gtcctgatta gcatacttcg gcgctgtccc atgggtcgct tcgaaaacgg
     3061 catgaccggt gatgtaattg atgttgccac cgggcgcgat gccaatgccg cccacttgcg
     3121 cagcgagtgc gtcgctcagg taatcgccgt tcaggttcag cgttgcgatt acatcaaaat
     3181 cttcgggccg cgttagcacc tgttggagcg tgatgtctgc aatggcgtcc ttgatcacaa
     3241 tcccggcacc agggttgccc atcggaattt tgcaccacgg gccaccctcg atttcctccg
     3301 ccccgaaata ttgcttcgcg atttcatatc cccagtcgcg gaatgcgcct tcggtgaatt
     3361 tcataatgtt gcccttgtgc acgagcgtca cgctcttgcg ctggttctgg atcgcgtagg
     3421 aaatcgcact gtgaatcaag cgcacgctgc ccgatcggct gaccggtttg atcccgatcc
     3481 ccacgcaaac ttcgtcgctt tccggcgcgc cgaccctttt ccagaattca gccgcttttt
     3541 ccttcgtgcc gaaacgaatc ttatcgaact cttttggaaa ttctttttgg aaaaaattga
     3601 ggatcttttg cgcttcgggc gttccggcgg cgtattcgat gcccgcataa atatcttccg
     3661 tgttttcccg gaagatcacc atgttcactt tctccgggtg cttgacgggc gaggggaccc
     3721 ctttgaagta ctgcaccggc cgcaagcaaa cgaaaagatc gagaagctgc cgcagcgcca
     3781 cgttgagcga gcggatcccg ccaccgaccg gcgtggtcaa tggccctttg atgccgacca
     3841 gaaattcacg gaacgcgtgg actgtatcgt cgggcagcca ctcatcgaat ttgtcttttg
     3901 ccgcttgacc agcgaagact tcaaaccacg cgatcttttt cctgccgcca aaagcttttt
     3961 ctaccgcggc atcgaacacg cgcacactcg cggcccagat gtccggcccc gtgccatctc
     4021 cacgaatgaa aggaataacc gggcggtccg gaacattcag tttgtcggtt ctggtaatct
     4081 tttcacctgg aggcggagca acatctttgt aaggcataag gggcaaactg tagaggcggc
     4141 tctcctcagc cgcaatcccg ctgtaaagac ggttgtcctc cgccgttaga aatttacgcg
     4201 agggtgatct cttacccgcc ggcaatatga ctactggctt taggccagtc gatccatcgg
     4261 caattcgccc gggctcagcg cattactcgt gtccggctaa aaactttccg agccgccccg
     4321 agcgctattc gtcgcttgat tgcgtaggct gttcggggga aacaaccgca gaggaaaagt
     4381 tagctgcatt aatcgtggtg gggatcgccg gttgaacgaa agcgttcgcc tcttgacagg
     4441 gctgagcgat gttcgctgcc gccacaatgg aatcgcgcag tgaaggcgcg gccgctactg
     4501 cagcgctgac aatttctctg gtcgcggcgg gatgtgcggc gatcgctcct cgaataatac
     4561 agtcagctga ctcggggccg gccgattggc tggcgcctag cgcggcggtg acaattctcc
     4621 ccgcaagatc aggccgagct cttactgccg ccgatgcata accgcatacc tcttgtgaac
     4681 tggagcgggc gaagagcgaa ttgagcgcgc gcatgaattg gtcaggcgtt gctgtgcccg
     4741 ggctactggc cccggccacc cttatcgctc gcgttaaatc aggattactg cttaccccgt
     4801 cctgagcccc atgctgcctt tcaatctgct gctcgattaa aggcgcgctt ggcaagcgcc
     4861 ggaaatcttt gatgagcggc gaagtgagca cctgattaag atcgacatcg accgggtctt
     4921 ctaaccggtt ggtgaccgga tcaaaagtca gtttttggcc ggcgtgaacc tgaagcgatt
     4981 gccccacgct atccagacgg atgcacgcgc tgccttcaag cacgatgaat gaggcgattc
     5041 cgctgggatc aacgttgccc agcaccgttg tgcctgtgat cgctgccgtg accggcccca
     5101 ttttgacttt tgcgccgcca gatttctttg ggacatacag caaaaattgg ccgctgctca
     5161 gctcaatggt tcgcgcgcct tcgcccacgt taaagatcgt cttttcgccc agacgcgtga
     5221 tcgttttatc cttgaacgtc agttcgctcc gggactggac accggtttgc acccccgtgc
     5281 cctggcgaac attgtcgttg actacagctg gacgcggcgc ggcgtttgaa gggagcactt
     5341 taacgtcctg gatgacctga gtcaccttgg cctccttgag atcctctgcg gctggcgcac
     5401 ttgggagaca gatcgcaaac gcaaacacaa gcggcgggat cgtcaaaatg gcccggggtc
     5461 ggatattcat gatctccttt atattccatt ccacatcgct tgttaagctt ttttttcggc
     5521 ttttcagcca taccgcgatt accggcgact cggctgccga atgcagcggg tcgccgcggc
     5581 gactgcgccc ccgaactcgg attctttcga ccccaaacat aaagcttttc cagcgttaaa
     5641 cttctgctag actctgcacc cccaacagtg tttaccccgg tcgtcagtcg tcagcctcct
     5701 gcgcgccaag cgtcccggta ccgcagccaa aaatcccgcg ggaccgtatt ctcgagcgaa
     5761 cttccctcgc cagtttccgt agaacgaact gtgaaggagc cttttctccc gctttctcgc
     5821 cgacttgatt accgatcacc gatcatccgc cttcgcttag ctacggcgag gcgggctgat
     5881 taccatccac tcctgccacg tgagcgcgcc agcgcgttta cgttgctcga actattggtc
     5941 gtcatcggca tcatcgcaat tttgatggtg ctgctggttc cggccttcac gagcatcaaa
     6001 ggcggcaccg acgtcactaa cgctgcgtac accatcaaag gcgtactcga cactgcccgc
     6061 acgtacgcga aagcaaacaa tacctacacg tgggtcggct tttacgagga agacgtctcg
     6121 caaccgtcaa ccaatccacc gacgcccgga acgggacggc ttgtcatgtc aatagtcgcg
     6181 tcaaaggatg gaacgaccgt gtataatccg aacagcactt tgaatccgga tcccatcgat
     6241 ccgacgaagc tgacgcaggt gaacaaatta gtgaagatcg acaatgtcca tcttccgctt
     6301 ttcgccgtct gcacggccaa ctgctcggga gacacttttg ataca
//
LOCUS       DASQUQ010000070         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_101240, whole genome shotgun sequence.
ACCESSION   DASQUQ010000070 DASQUQ010000000
VERSION     DASQUQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101240"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VE486_02000"
     CDS             complement(<1..971)
                     /locus_tag="VE486_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37891.1"
                     /translation="MRANCFLFLISALTGLTLIDLSAIEAAPQTPKKPLSDEYHGVTV
                     QDPYQWLENDEDPEVKTWSDAQSQRTRAYLDKLPDRAALEKQLTEWYAKTSPSYSSLV
                     SRPGILFAIKFQPPKQQPLLVTLVSADDLKSEKIVLDPNVLDAKGTTAIDWFVPSLDG
                     RHVAISLSKGGSEDGTLHFYETATGKALPDTIAHVQYPTAGGSAAWNADSTGIYYTRF
                     PRKGERPEADLNFYQQVYFHKLGTPDTEDAYSIGKDFPRIAEIVLEAARDGKYILATV
                     ANGDGGDFAHYLLGPDGTWKQITQFSDQVKAARLGRDKALYLLSRADA"
     gene            complement(1016..1651)
                     /locus_tag="VE486_02005"
     CDS             complement(1016..1651)
                     /locus_tag="VE486_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HZA37892.1"
                     /translation="MVFVFGGTIGLASELPTSVDSKDGWKLASENKDVSIYSRAHAGS
                     RLKEFRAIGPIDAPTSAVHAVIDDFENYPNFMPYTLECRLLKRESDSIITYQRLSPKI
                     AEDRDYTLRVWKKSWPGPDGFTYLDQWKTANELGPAEKKGVVRIEICNGGWLLEPDGP
                     SKTRATYSVYTDTGGLIPAFLANYASRTGISRLFEAVRKQVKNPRYGVASR"
     gene            1835..>2482
                     /locus_tag="VE486_02010"
     CDS             1835..>2482
                     /locus_tag="VE486_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZA37893.1"
                     /translation="MSPQPTILIVDDEKHTRDGLRRLLEDDYDVYVAADIAGAVSVLE
                     REQIDLLLTDLRLGSEDGMQLIDRALKMPRPPICIMMTAYGSIDVAVEAMKRGAYDFV
                     TKPLNLDKVELLIARAVQSRKLEQENRALRQQVDERYGLENIWGDSAALHEVLDTIRQ
                     VAPSSANVLIEGESGTGKELAAHAIHNLSRRNKAKFVAVHCAALSPQLLESELFGH"
BASE COUNT          546 a          691 c          680 g          565 t
ORIGIN      
        1 gcatccgcgc gtgaaagaag atagagtgcc ttgtcgcgtc ccaggcgcgc agctttgacc
       61 tgatcgctga attgcgtgat ctgcttccac gtcccgtccg ggccgagcaa ataatgggcg
      121 aaatcgccgc catcgccatt cgccactgtt gcgagaatgt attttccgtc acgcgccgct
      181 tcgagcacga tctccgcgat tcgcggaaag tctttgccaa tcgaataagc gtcttcggtg
      241 tcaggcgtgc cgagtttgtg aaaataaacc tgctgataaa aattcagatc cgcttcgggc
      301 cgttcgcctt ttcgtgggaa acgagtgtaa taaattccgg tgctgtccgc attccaggcc
      361 gcgctaccgc ctgccgtggg atattgcacg tgtgcgatgg tgtcgggcag cgcttttccc
      421 gtcgcagttt cgtagaagtg aagcgtgccg tcttcgctgc cgccttttga aagcgagatc
      481 gcgacgtgcc tgccatccag tgatggaaca aaccaatcga tcgccgtcgt gcctttggca
      541 tccagcacgt tcgggtcgag cacgatcttt tccgacttga gatcgtcggc cgaaacaagc
      601 gtcaccagga ggggctgctg ctttggcggc tggaatttta tcgcaaacaa gattccgggc
      661 cgcgagacga gcgaagaata actcggcgaa gtttttgcgt accactccgt taattgcttc
      721 tcgagcgcag cgcgatccgg caacttgtcg agataagcgc gagtccgctg gctctgcgcg
      781 tccgaccacg ttttcacctc cgggtcctcg tcgttttcca gccattgata cggatcctgc
      841 acagtcacgc cgtggtattc gtcggaaaga ggtttctttg gagtctgcgg cgcggcctcg
      901 atcgcggaca agtcgatcag cgtcagtccg gttagcgcac tgatcaaaaa caggaaacaa
      961 ttcgctctca ttaacaaagt ttagacaaag gacgaaatcc ggggccgagg cggcttcaac
     1021 gactcgccac gccgtatctc ggatttttga cctgctttcg aaccgcttca aacagcctgc
     1081 tgattcccgt ccggcttgcg tagttagcga gaaacgccgg gataagtccg cctgtgtcgg
     1141 tgtaaacgga gtaagttgcg cgtgttttgc tcgggccatc tggctcgagc agccaacccc
     1201 cgttgcaaat ctctattcgg acaacccctt tcttttcagc gggtcccaac tcgttggctg
     1261 ttttccattg atcgagataa gtaaatccat ctggaccggg ccatgacttt ttccaaacgc
     1321 gcagagtata atcgcggtcc tcagcaatct tcggcgacag ccgctgataa gtgatgattg
     1381 aatcgctttc gcgtttcaag agacggcact ccaaggtata aggcatgaag tttggataat
     1441 tctcgaagtc atcgatcact gcatgcaccg cagatgtcgg agcgtcaatc gggccgattg
     1501 cgcgaaactc cttcagtcgt gagccagcat gcgcacgact gtagatggag acatccttgt
     1561 tttcgctggc tagcttccaa ccgtctttgg aatcgaccga agtcggaagt tcggaagcga
     1621 ggccgattgt tccaccaaag acaaagacca tcgcgaatcc cgctagaatt cgcccctgca
     1681 tcacccgacc atacccgatt atagcggtcg cgccattttt ctgtagtcgt cgtcaaaggc
     1741 gttgagagat tcagtgattg agtgattaag tgattgaagg aaagtaatgc tcactcgcta
     1801 actcgttaaa tcgccgaatc gtcaaaatcg caaaatgagt ccacagccta ccattctcat
     1861 cgtcgatgac gaaaagcata cgcgcgacgg tttgcggcgt ttgctcgagg acgactacga
     1921 cgtgtatgtc gcggcggata ttgcgggtgc agtgagcgtc ctcgagcgcg agcagatcga
     1981 tctgcttctc accgatctgc gcctgggcag tgaagatgga atgcaattga tcgatcgcgc
     2041 tttgaaaatg ccgcgacctc ccatctgcat catgatgacc gcatacggct ccatcgacgt
     2101 cgcggtcgaa gcgatgaaac gcggcgccta cgattttgtc accaagccgc tcaacctcga
     2161 caaagtcgaa ttgttaattg cgcgcgcggt ccaaagccgc aaactggagc aggaaaatcg
     2221 tgcgctccgc cagcaagtgg acgagcgcta cggccttgag aacatttggg gcgactcggc
     2281 ggcgttgcac gaggtgctcg acaccatccg gcaagtcgca ccctcctcgg ccaatgtgct
     2341 catcgaaggc gagagcggca ccggaaagga attggccgcg catgccattc ataatttgag
     2401 ccgccgcaac aaagcgaagt ttgtggccgt gcattgcgcg gcgctctcgc cgcaattgct
     2461 cgagagcgaa cttttcgggc ac
//
LOCUS       DASQUQ010000071         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_828937, whole genome shotgun sequence.
ACCESSION   DASQUQ010000071 DASQUQ010000000
VERSION     DASQUQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828937"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VE486_02015"
     CDS             complement(<1..603)
                     /locus_tag="VE486_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZA37894.1"
                     /translation="MKSSKSDAPKPRRQLGLFDAIMIVMGGIVGAGIFANPSEVAHRV
                     HTPFLILGVWVLGGFIAMCGAFIWAELATRLPGTGGQYLYLREAYHPSVAFVYGWGLL
                     LVTQTGGMAAVAVIFSSYFRALTGVVWNDSAIAAVVLLILTGINCLGARAGSNVQSAL
                     MLLKIGALAALVTLGFALGGGSLKSGPLLAEPVSFGLVKSI"
     gene            733..1344
                     /locus_tag="VE486_02020"
     CDS             733..1344
                     /locus_tag="VE486_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37895.1"
                     /translation="MKTLPLAEAQTSRSPHRRAAVSSSSRGLKVCATIMLALLLIFVA
                     PLHAQDEPKLVPDAVDYPKLLPVLPDAPEGWTADKAEGSTDDVGGFRITNVHRDYRKG
                     EGDKAPIAAISILDSAANPDYVSATIEAWHNASETSEGYSKSVTIDGNPGFETFENES
                     KHATLWVMIANRYFLQIELQEQEPKQLQEWVKRVDLKKLAAIK"
     gene            complement(1427..2146)
                     /locus_tag="VE486_02025"
     CDS             complement(1427..2146)
                     /locus_tag="VE486_02025"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZA37896.1"
                     /translation="MARPLQFLIIAVIAAGGCGQKPTAASDFTGERYQMVQQQLKARG
                     VSDPRVLAAMNKVRRDEFVPPNLKGSSYADQPLPIGYNQTISQPYIVAFMTEQLRLSP
                     KDRVLEIGTGSGYQAAILAELAAEVYTIEIIEPLAKTAEATLQRLGYKNVHVKAGDGY
                     KGWPEYAPFDAVIVTAAPDHVPQPLIDQLKEGGRMIIPVGSGLGFQQLHLLEKKNGKL
                     QRRAVLPVRFVPMTGEAQKPK"
     gene            2279..>2921
                     /locus_tag="VE486_02030"
     CDS             2279..>2921
                     /locus_tag="VE486_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37897.1"
                     /translation="MLEERKTLEQREQMTDIERLRHSASHVLATAILKIWPEAQFAAG
                     PPVENGFYYDVDLPHRISPDDFEKIEAEMKKEIKANHPFERMEVSRDEALEMGKKGRL
                     AALSERPEPSKYKIDIIENIPPDEKISLYRNGDFIDLCAGPHVMRTGNVGAFKLMNVA
                     SAYYKGDERNPQLQRVYGTAFKTKKELDDYFAMIEEAKKRDHRKLGKELELFVF"
BASE COUNT          718 a          847 c          769 g          587 t
ORIGIN      
        1 aatgcttttc acgagtccga acgaaaccgg ctctgcaagc aacggccccg acttgaggga
       61 tccaccgcca agcgcaaatc ccagtgtcac gagtgccgca agcgccccga tctttaacaa
      121 catgagcgcg ctttgcacgt tgctgccggc gcgcgctccc agacagttaa tcccagtcaa
      181 tataagcaga accacagctg cgattgcgct gtcgttccaa actactcccg tgagcgcgcg
      241 aaagtaagat gagaaaataa ccgccacagc ggccatcccg cccgtctgtg tgacgagcag
      301 caggccccat ccatacacga acgcaacgga aggatgataa gcctcgcgca agtaaaggta
      361 ctggccgcct gttcccggca aacgcgtcgc cagctcggcc cagataaacg cgccacacat
      421 cgcgatgaag ccgcccagca cccagacgcc gagaatcaaa aacggcgtgt gcacgcgatg
      481 cgcgacctcg gagggattcg cgaaaattcc cgcacccacg atgccgccca tcacaatcat
      541 gatggcgtcg aataatccaa gctgacggcg aggcttcgga gcgtccgatt ttgagctttt
      601 cattttggcg ctatctcgtt cggcgcgccc tcgccacggc gagcaggctg tggtcgcgcc
      661 ctaccaatgc gcatcatgca accgggtctt gacaaacagc cccgctaaag tcaatcgagt
      721 acgtgggccg aaatgaagac gctacctctt gctgaagcac agacatcccg ttcgccgcac
      781 aggcgcgccg cagtgagttc atcctctcgc ggactgaaag tttgtgccac aatcatgctc
      841 gcgttgctgc tcatcttcgt agctccgttg catgcccagg acgagccgaa gctcgtgcct
      901 gacgccgtcg attaccctaa attgcttccc gttttgccag acgcacccga aggatggacc
      961 gctgataagg cggaaggctc aacggacgac gttggcggct tcagaatcac gaacgtgcat
     1021 cgcgattatc gcaaaggcga aggcgacaag gctccaattg cggcgatcag catactcgac
     1081 tcagcggcga atcccgatta cgtcagtgcc accattgagg cgtggcataa cgccagcgaa
     1141 accagtgaag gttacagcaa atcagtaacg atcgatggta atcccggatt tgagacgttc
     1201 gaaaatgaaa gcaaacatgc caccctgtgg gtgatgattg ccaatcgcta ttttcttcag
     1261 atcgaattgc aggaacaaga gcccaagcaa ttgcaggaat gggtcaagcg tgtcgatctg
     1321 aaaaaactcg ccgcgataaa gtagtgatcg gtgaacagtg atcagttatc gggcgatggt
     1381 cagcacccag cgagggcaca ctgttcacca gatcactaat cactgatcac ttcggtttct
     1441 gcgcctcccc cgtcatcggc acaaagcgaa caggcaaaac agcgcgccgc tgtagcttgc
     1501 catttttctt ctcgagaaga tgcagctgct gaaatccaag accgctcccg actggaatga
     1561 tcatgcgacc gccttctttc agttgatcga tcagcggttg tgggacgtga tctggcgccg
     1621 cagtgacgat aactgcatcg aaaggcgcat actctggcca gcctttgtaa ccgtcgcctg
     1681 cttttacgtg cacatttttg tagccaagac gctggagcgt cgcctccgcc gttttcgcca
     1741 gcggctcaat aatttcaatc gtgtaaacct ccgccgccag ttcggccagg attgctgcct
     1801 gataaccaga gccagtgcca atttcgagaa cgcgatcctt cggcgacagt cgcaattgct
     1861 cggtcatgaa cgcgacgata tagggttgcg agattgtttg gttgtagccg atcggcagcg
     1921 gctgatcggc gtaacttgat cccttcagat tgggcggcac aaactcgtcg cgtcgtacct
     1981 tgttcatcgc agccaacacg cgtgggtcgc tgacaccacg cgctttcaac tgctgctgaa
     2041 ccatctggta gcgctcgcct gtaaaatcgg aggctgccgt tggtttttgc ccgcaaccgc
     2101 ccgcagctat caccgcaata atcagaaatt gcaaaggtcg tgccatggca aaaaatacat
     2161 cgcaccgcac gacgcgaaag gcggactttt ttaaccatca agtttcctcg agaattcgga
     2221 tcgacaaatt cttcgtgtcc ttcgtgccct tcgtggttaa tttcacccac tttcaaccat
     2281 gcttgaagag cgcaaaacat tagagcaacg cgagcagatg acggatatcg aacgtctccg
     2341 tcattcggcg tcgcacgtac ttgcgacggc aatcttgaaa atttggcccg aggcgcagtt
     2401 cgcggccggg ccgccggtcg agaacggctt ctattacgat gtcgatcttc cgcaccgcat
     2461 ttcgcctgat gatttcgaaa agatcgaggc cgagatgaag aaggagatca aagcgaacca
     2521 tcccttcgag cggatggaag tttcgcgcga cgaggcgctc gagatgggaa aaaaaggccg
     2581 gctcgctgcg ctaagcgagc gacccgagcc gagcaaatac aaaatcgaca tcatcgaaaa
     2641 cattccgccg gacgagaaaa tctcgctcta tcgcaacggt gacttcatcg atctctgtgc
     2701 cggtccgcac gtcatgcgca cgggaaacgt tggcgcattc aagctcatga acgtggcgag
     2761 cgcttactac aaaggtgacg aaagaaatcc gcagctccag cgcgtttacg gcaccgcctt
     2821 caagacgaag aaggagctag atgattactt cgcgatgatc gaggaagcga agaagcgcga
     2881 ccatcgcaag ctcggcaaag aattggagct attcgttttc g
//
LOCUS       DASQUQ010000072         5025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_281945, whole genome shotgun sequence.
ACCESSION   DASQUQ010000072 DASQUQ010000000
VERSION     DASQUQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281945"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1228)
                     /locus_tag="VE486_02035"
     CDS             complement(185..1228)
                     /locus_tag="VE486_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZA37898.1"
                     /translation="MAQPQAEGFPVVTARRGKQDEPANGRDGPPGRPKRWRENRGAPG
                     GHALPTPMIGEGTQSTNQRRHNFVQEHVEVGFFTDPTVCIGCKACEVACKEWNHVPDD
                     GFVWSGNSYDNTGHLGASTWRHVMFVEQDRRKGNQITGLMGLVNQKSEIRDQQFDDPF
                     RWVFLSDVCKHCEEAGCLEACPTGAIVRTEVGSVLVQNDVCNGCGYCVVSCPFGVIDR
                     RPQSLSEAGGAFKCTFCYDRQKSGLVPACAKVCPTESIVFGRRDDLRARGAERVQQLQ
                     QIGYNDAQLYDPQETSVRGIHAFFLILGEPEAYGLPPKPQIPTIYLKSAWTSAFATAI
                     ASLIVTLLAFAFL"
     gene            complement(1305..4421)
                     /gene="fdnG"
                     /locus_tag="VE486_02040"
                     /pseudo
     CDS             complement(1305..4421)
                     /gene="fdnG"
                     /locus_tag="VE486_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_271413141.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
     gene            complement(4648..>5025)
                     /locus_tag="VE486_02045"
     CDS             complement(4648..>5025)
                     /locus_tag="VE486_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37899.1"
                     /translation="IDSDIVDTVAELQKLGGARVILATAPSAKAISEVVDGLSVNGNL
                     LVPAAPAEPLTVSVMPLLMGRRSVSGWYSGTAQDSQDALEFSALTGVHPMIEKYSLSR
                     VAEAYEQMHSGKVRFRVVLTMGK"
BASE COUNT         1035 a         1465 c         1381 g         1144 t
ORIGIN      
        1 gatctgcatg cgttttctgg gtagcggtcg ccgcggcgac ctggcgaatg cgttctgcat
       61 tcgcgaacct tcttttggat tctcgtcatt tctgatagcg gaatccaaag aaaagtttgt
      121 gaaggcaaaa tgccttcacc agcacgcaga atgcgtgcgc tccccagaat tgtcgatcgc
      181 cgcctcataa aaatgcgaat gccagcagtg tcacaatgag cgaggcgata gcggtcgcga
      241 acgccgaggt ccatgccgac ttcagataaa ttgttggaat ctgcggcttc gggggaagcc
      301 cataggcttc cggttcaccg agaataagaa agaatgcgtg aatcccgcgc acggatgttt
      361 cctgcggatc gtaaagttgc gcgtcattgt aaccaatctg ttgtaattgc tgcacgcgct
      421 ccgctccacg cgcgcgcaaa tcatcgcgcc ggccgaacac gatcgattcg gttgggcaaa
      481 cttttgcgca cgccggaacg agcccgcttt tttggcgatc gtagcagaag gtgcatttga
      541 acgcgccgcc ggcctcagac agcgattgcg gtcggcggtc gatcacgccg aacggacacg
      601 agacgacgca gtagccgcat ccgttgcaga catcattctg tacaagaacg gaaccaactt
      661 cggtgcgcac aatggcgccc gtggggcatg cttcgaggca accggcttcc tcgcagtgtt
      721 tgcaaacatc ggaaaggaaa acccagcgaa acgggtcgtc gaattgctga tctctgattt
      781 ctgatttctg attaacgaga cccatgagtc ccgtgatctg gtttcccttc cgtcgatcct
      841 gttcaacaaa catgacgtgc cgccacgtcg acgcgccgag gtggccagta ttatcgtaag
      901 aatttcccga ccaaacgaag ccgtcgtccg gcacgtggtt ccactccttg cacgcgactt
      961 cgcacgcttt gcaaccgatg cacactgttg ggtcggtaaa aaaaccgacc tcaacgtgct
     1021 cctgcacgaa attgtgccgg cgttggttcg tgctctgtgt gccttcgccg atcatgggag
     1081 ttggtagggc gtgaccgccg ggcgcgccgc ggttctcgcg ccaacgtttc ggacggcccg
     1141 gcggtccgtc cctaccattt gcaggctcat cctgttttcc cctccttgcc gtgactacgg
     1201 gaaaaccttc cgcctgtggc tgcgccaaga ggcggttctt cgggatgcag aatcggcgac
     1261 cctgtttgcg gcgcgtactt gttgaggaac tcggcgaagc cgcttccccg cggcaaacga
     1321 ccgggcacga tgttgcaggt gagcgccttg gcttccatga tcgtgacgtt cggttcgcca
     1381 gaaattgaga gaagatcatt ggctgcgccg ccgcggacca agccggctgt cccgtagtga
     1441 tatggcaatg cgatctggtg cagtctcttc ccgtttatgc gcagcggccg aatccggcga
     1501 ctaacgaggg cccgcgcttc aatggccgcg cgtaaagtaa caatgcaaat atgatcgcca
     1561 ttattgatct tcagctcgcg cgcgagctcg ggcgacatct ccgcgaacat ctccggctgc
     1621 aattccgcga gatgcgacag gtagcgggac attccgccgg cggtatggtg ctccgttagc
     1681 cgataagtcg ttaacacgaa cggaaaccgc gggtcgccgg gcggcgcgaa ccgattttcg
     1741 ccacgcggaa accaatgcac cgcggggttc gtgtcgtgcg agtaaagttc atttgagaca
     1801 ggcgactcga gcggctcgta atgagtcggc aacggcccgt cctgcaaacc ttttggcaca
     1861 tagagccagc ctagtccgtc ttcgtgcagg ataaacggtg catcaccggg aatcgcatcc
     1921 agtccttccg cgccttcctc tggttgatag tccggagctt ttcctttcac aaaaccggca
     1981 acgtctattc cggtccattt ttccgagtcc caccacacca atttttttcg ttcgctccag
     2041 ggactgccgt ccggccgggc actggcgcga ttgtaaatga ttcggcgatc gctcggccat
     2101 gcgaagcccc agccgtgacc gagataacct ctggacttgc gtgagtttgc cttgttcact
     2161 ccgtcgcgac caaaaacgcc ggagtaaatc cagcagccgg atgcagtcga gccgtctgct
     2221 ttcaattccg cgaaaccatt tacctgttgc ccgtgatgca cttggccgtc tcgatcaacg
     2281 ccgaatacaa cgcccgccgc gctgccgggg tcaccgaccc cggctacagg tttggtttgc
     2341 cagccgttga tctcggcgag cacggcttcc attttcggat cgcccagttg gtcttccgga
     2401 taccaccagt ccagagcgcg cagcggttca tcgttcgggt catttgacgc ttttgccttc
     2461 gcgatcaagc gcaacgcgag ttgatgcata aaccaggcat ccgagcgaca atcaccgggc
     2521 ggatcgacgg ctttttcgcg ccattgcaac agtcgctgcg tgttagtaaa cgcgcctgct
     2581 ttctcggcat gcccggcagc ggggaaaaag aaaacttcag tctcgatgtc atcggctttc
     2641 aactcgccgc gctcgatctc gggcgaatcg caccagaaat ttgccgattc gatttccacc
     2701 atgtcgcgca ccactaacca tttcagtttc gacaacgctt ttcgctgaag ccgcgagttg
     2761 ggcgcgccga cggctggatt ctgtcccatc aaaaacatgc cctccatttt tccgtcgagc
     2821 atgtcgtaaa gaaattcgaa gaaggaatgg ttgccggtaa gtttaggaag ccaacagtag
     2881 ccaaaatcgt tttcggcagt cgcgtgcttt ccgtagtagg ccttgagcgt gctcaccaag
     2941 tatttcggat agtccgccca aagtccggtc tttttcgtgt agtgatcgag gtactgctga
     3001 agagtttcgt cacctttgtg cggcattgtc agatagccgg gtagaatgtc gtaaagcgtg
     3061 ggaatatcag ttgagccctg gatcgaagcg tgaccgcgta acgcgagaat tccaccgcca
     3121 ggccgtccga tatttcccag cagcaactgt aagatcgcag ccgtgcgaat gatctgcacg
     3181 cccttggaat gctgcgtcca gccgaccgcg tagcaaaccg cagcggtttt gtccggtccg
     3241 gatgcggcaa cgagcgcgtc agcgaccttg tgaaagagtt cgggcgagat gccgcagact
     3301 ttctccacta tttcaggcgt gtaccgagaa aagtgtcgtt ttaatttttg aaaaacacat
     3361 tgtggatctt gcaatgtgag gtcgcgctgt ggacggctca gatcgccgcc tttgtagagc
     3421 caactgtcgc cttggtaggc gcgcttcgcc tcgtcccaac cggagaaata gccgtcgccg
     3481 ttatcttcag ggtcgcggta ctcatcggac aaaatgcagg aggcattcgt gtaatggagg
     3541 acgtactcgc gaaagaagag ttcgttttcg accaggtgtc gaatgagacc accgagaaac
     3601 gcgatgtcgc ttccggcgcg gatcggcacc caaatgtcag ccagcgcgct ggtgcgcgaa
     3661 aatcgtggat cgacatgaat gatggttgcg ccgcgttctt tcgctttcat cacccagcgg
     3721 aaaccgaccg ggtgcgcctc ggccattgag gagccttcga ttaagataca atcggcgttg
     3781 gcgagatctt gttgcgcggt tgtagctccg ccccggccaa agctggtgcc cagaccgggc
     3841 accgtgctgc tatgtcatat ccgggcctgg ttggatacgc agaccatgcc caggcctcct
     3901 gtgaaaagct ttttgatcag ataattttct tcgttatcga gcgtggcgcc gccgagatgg
     3961 cagatcgcag tcgtatgatt gatggtcgcg ccattttgtt tgtgcacaaa cgtacgcttg
     4021 cgtgattccc agacgcgatc cgctaccaaa tccatcgctt ggtctaacga aatctgtgac
     4081 cactctttcg cgcgcggcgc gcgatatttc attttcatct cgcgccgatc atgcgtaagc
     4141 agctgatatg atgccgcgcc tttcgggcaa agattcccct ggctgatcgg actctccgga
     4201 tcgccctcga tcgaaatcag tttcccctcc ttgtgataaa tcagttggcc gcaaccaacg
     4261 ccgcagtacg gacaaatcga gcgggccacc tgcgcatcgt cgatccgtgc gtgcattgca
     4321 cgggtcttat cggacatcgc ttccaatcct gtcccgtcag cccgctcacg aatttgacga
     4381 atgagcggcc atttcgagag caacgcggtg agcgacgtca tatcgtttag tcctcgctta
     4441 agcggccatt ttccctgcta tttgttttat cggcagcggt caatcataaa agagatccgc
     4501 gcccgacggg aagggaagtt tcctccgtca ttcatcgtct tttttatttg ggtggtgttt
     4561 ggcaattccg accgcaaaca cccccggaga ggtgagtgaa aggctcaaat cagtgctcgg
     4621 tccgcattta gtgctacgac cgccctgtca tttgcccatt gtgaggacga cgcggaagcg
     4681 cactttgccg ctgtgcatct gttcgtaagc ttccgccacg cggctcagcg aatatttctc
     4741 aatcatcggg tgcacgccgg ttagcgcgct gaactccaaa gcgtcctgcg aatcttgcgc
     4801 tgtgcctgaa taccaaccgg acacggaccg ccttcccatg agtaacggca tgacactcac
     4861 cgtcagcggc tccgccggcg cagccgggac taacaaattg ccgttcaccg ataacccatc
     4921 cacgacttcg gaaatcgctt ttgcgctcgg cgccgtcgcc aggatcacac gcgcgccgcc
     4981 gagcttttgc aattccgcta cggtatcgac aatatctgaa tcaat
//
LOCUS       DASQUQ010000073         4332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_830900, whole genome shotgun sequence.
ACCESSION   DASQUQ010000073 DASQUQ010000000
VERSION     DASQUQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830900"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VE486_02050"
     CDS             <1..818
                     /locus_tag="VE486_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZA37900.1"
                     /translation="KTVHRVLDPNKPASIPGNLCALAVMTKAPRAGEVKTRLVPPLSH
                     DEAAQLNRAFLRDICAVISAACWRGPESTRDRIRVARGVGVYTPLGTESAYENVLSDD
                     FFLIPQRGDHFGDRLNFSAEDLFKVGFDSVCLINSDSPTVPAEVFAQAVELLGLRGDR
                     IVLGPSDDGGYYLIGLKEPHPEMFERIDWSTERVFDQTLARATELGVEVKLLPTGYDV
                     DDHATLQQLCSELLGENSRDGVAPNTRKVLREIIEREGRAGIWPGAASRSGGF"
     gene            920..2074
                     /locus_tag="VE486_02055"
     CDS             920..2074
                     /locus_tag="VE486_02055"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZA37901.1"
                     /translation="MPMLRSGNKMKRALVTGGAGLIGSHVTDLLLAEGWKVRVLDNLE
                     PNTHKRGKPAWINPAAEFIEGDLRDRDTITAALDKIDTVFHQAAYGGYMPEIAKYVHV
                     NSLGTAQMLEVIRENKLPIRKIVVASSQAVYSEGAGECPEHGLVFPPVRPVEQLRRGD
                     WQVHCPICSAITKSVPTPENAPVGGETVYGLTKVDQERLVLLWGKQIGIPTVALRYSC
                     TYGPRQSIFNPYTGVIAIFCTRLLNNLPPVLYEDGEQTRDFSFVEDIARANLLAAETD
                     RLDGLPVNVGSGRGVSIREIAETISNALEVNIAPEVNGEFRPGEMRHLTSDTTRIRAL
                     GYEAQVDLADGIGRYIDWIKAQSDVRDYFSEAAHILRGKGIVHRVEMVND"
     gene            2067..3083
                     /locus_tag="VE486_02060"
     CDS             2067..3083
                     /locus_tag="VE486_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZA37902.1"
                     /translation="MTEAVGIPDFDSPVIEHARKDFPLLDADMTVGEALERIRREGVG
                     ERVIYFYAVDAEKRLVGVLPTRRLLTAPLENSLRDIMVRRVIAIPAKATVLDACEFFV
                     LYKFLAFPVIDEQRGVVGVIDVSLFAEEMLQAGESEDRHRPAAPVHDDIFEALGLHLA
                     QIRGASPWRVFRFRFPWLLVTVTAGTACALLTGAFEATLARSLVLAFFLTMVLGLNES
                     VSAQSMSVTIQALRSATVTWQWFATAFRREIQTAGLLGLACGTVVGVIVWLWRSDPHA
                     AFVIGGSIALSLVTACLFGLGVSSLLHRFKLDLKIAAGPLTLALADIFALLIYFTSAW
                     LVLR"
     gene            3080..3793
                     /locus_tag="VE486_02065"
     CDS             3080..3793
                     /locus_tag="VE486_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZA37903.1"
                     /translation="MSAVSVIIPALNEEEPIADVVREVTATNIPAEIIVVDNGSTDRT
                     AERARDAGARVVSEPIPGYGRACMAGIRALAPQCDIVVFLDGDGSDCPEFMNQLVDPI
                     ASGTHDFVIGSRTRGEREPGSMNFQQIVAGRMAGLILRLLYGVRFTDMCPFRAIRHDA
                     LEKLGMREQTYGWNLEMQMKSARVGLRILEIPVNHRRRAGGESKVSGTLRGTFIAGAR
                     IIATLLRIATERKPRMNTD"
     gene            3880..>4332
                     /locus_tag="VE486_02070"
     CDS             3880..>4332
                     /locus_tag="VE486_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37904.1"
                     /translation="MTLPDELSRGKAIATGLLAGLVAAIAMTVMMLLLACFGVATPLV
                     IIGDRLSVFIPPGPFLALMGKVGGYNHLKQLGVGSTIAGQLIVGAIGGAIFGFCMRRN
                     PGKPATLATIAVFIALPLIGLVIALWPVLGTSYVGLPIAAARLVTVVGF"
BASE COUNT          841 a         1213 c         1301 g          977 t
ORIGIN      
        1 tgaaaacagt tcatcgcgtt ctcgatccga ataagccggc atcgatcccc ggcaacctct
       61 gtgcgctggc ggtaatgacc aaagcgccac gcgccggaga agtgaaaacg cggcttgttc
      121 cgccgctctc acacgatgaa gccgcgcaac ttaacagagc gttcctgcgc gacatctgcg
      181 ccgtcatttc cgcagcctgc tggaggggac ccgaaagcac tcgggatcgt attagagtcg
      241 ctcgaggtgt tggcgtttac actccgttgg gcaccgaatc cgcttacgaa aacgttttat
      301 ctgacgactt ttttctcatc ccacagcgcg gcgaccattt cggcgatcgt ttaaattttt
      361 cagcagagga cctgttcaaa gtcgggttcg attctgtttg tctgattaac tccgatagtc
      421 cgacggttcc ggctgaggtt ttcgcacaag ccgtggaact gctgggtttg cggggagatc
      481 gcattgttct cggtccttcc gatgacggcg gctattactt gattgggctg aaggagccgc
      541 atcccgaaat gttcgaaaga atcgactgga gcaccgagcg agtgttcgac cagacactgg
      601 cgcgcgcgac tgagctcggt gtcgaagtga aattactgcc aaccggctac gatgttgatg
      661 accacgcaac gttgcagcag ttgtgcagcg aattgctcgg tgaaaactct cgcgatggcg
      721 tcgctccgaa tacacgaaaa gtcctccgcg aaatcatcga gcgcgaaggc cgcgccggga
      781 tctggccggg agcggcatca aggagcggcg gtttctagtc cgccgtccct ttccttgagt
      841 acgtccgctt gcgcgcgagg gcgcgcgaat ttggccgtgg cgtcggcttc cagccgatgt
      901 tcgggcttca tcggcacgga tgccgatgct acggtcaggc aacaaaatga aacgcgcgct
      961 tgttaccggt ggcgcgggcc tgatcggctc gcatgtcacc gatctgcttc tggccgaggg
     1021 ctggaaagtt cgcgtgctcg ataatctcga gccgaacacg cacaagcgag gcaaaccggc
     1081 atggatcaat ccggccgccg agttcatcga aggcgatttg cgtgatcgcg acactattac
     1141 ggccgcgctc gacaagattg acaccgtctt tcaccaggct gcctatggcg gttacatgcc
     1201 cgagatcgca aaatacgtgc acgtgaattc gctcgggacg gcgcaaatgc tggaggttat
     1261 tcgcgaaaac aagctaccaa taaggaaaat cgtcgtggcg agctcgcagg ccgtttacag
     1321 cgaaggcgct ggcgaatgtc cggagcacgg gttggttttt cctcctgtcc ggccggtgga
     1381 acaactgcgc aggggcgatt ggcaagtgca ttgtccgatc tgcagcgcga ttaccaaaag
     1441 cgttcccacc ccagaaaacg cgccggtcgg cggcgaaacc gtttacggtc tgaccaaagt
     1501 cgatcaagag agactcgtcc tgctttgggg aaagcagatt ggtattccga ccgtggcgct
     1561 acgttactcc tgcacctacg gcccgcgaca atcgatcttc aatccatata cgggcgtcat
     1621 cgcgattttc tgcacgcgtt tgctgaataa tttaccgccg gtgctttatg aagatggcga
     1681 gcaaacacgc gacttttcgt tcgtcgaaga cattgcacgc gcgaacctgc tggcggcgga
     1741 aacggataga ctcgatggat tgccagtgaa tgttggcagc ggccgcggcg tgtcgattcg
     1801 ggagatcgcc gaaacaattt cgaatgcgct tgaagtcaat attgcgccgg aagtaaacgg
     1861 cgaatttcga ccgggcgaaa tgcgtcatct caccagcgac acgacgcgca ttcgggccct
     1921 cggctacgag gcgcaagtcg atctcgccga cggcataggc cgctacatcg attggatcaa
     1981 ggcgcaaagc gacgtgcgcg attatttcag cgaagccgcg cacattttgc gcggcaaagg
     2041 cattgttcat cgagtagaaa tggtgaatga ctgaggcggt cggcattccc gatttcgatt
     2101 cgccggttat tgagcacgcc cgcaaggatt ttccgctcct cgatgcagac atgacggtgg
     2161 gcgaagcgct cgagcggata cggcgcgaag gtgtgggtga gcgcgtaatt tacttctacg
     2221 cggtcgatgc tgagaagcga ctggtgggag ttcttcccac ccgacggctg ctgactgcgc
     2281 cgcttgagaa ttcactccga gatatcatgg tccggcgcgt gattgccatt cccgcgaagg
     2341 ccaccgtcct cgatgcctgc gaatttttcg tgctctacaa attcctcgcc tttccggtga
     2401 ttgacgaaca acgcggcgtc gtcggcgtca ttgacgtgag ccttttcgct gaggagatgc
     2461 tccaagctgg cgaaagcgaa gatcgacatc gtcccgccgc gccggtgcac gacgacattt
     2521 tcgaagcgct cggtttgcat ttggcgcaaa tccgcggcgc gtcgccctgg cgcgtattcc
     2581 gatttcgttt tccgtggttg ctcgtcacgg taacggccgg caccgcctgc gcgcttctga
     2641 ccggtgcgtt tgaagccacc ctcgcgcgaa gtctcgttct cgcgtttttc cttacgatgg
     2701 tgctgggtct caacgaaagc gtcagcgcgc aatcgatgag cgtgacgatc caggcgctcc
     2761 gttccgccac ggttacgtgg cagtggtttg ccaccgcatt ccgtcgcgag atacagactg
     2821 ccggattgct cgggctcgcc tgcggcacgg tggtgggcgt gattgtttgg ttgtggcgat
     2881 cggacccgca cgctgcgttc gtcatcggtg gaagcatcgc gctgtcgctc gtgacggcgt
     2941 gcctcttcgg tctcggtgtg tcgtcgctcc tgcaccgctt taagcttgac ctgaaaatcg
     3001 ccgccggtcc cctgactctc gcgcttgccg atatttttgc gctcctcatt tatttcacca
     3061 gcgcctggtt ggttctgcga tgagtgccgt ttcggtcatc attccggcgc tcaacgagga
     3121 ggaaccgatt gctgatgtcg ttcgcgaagt cacggcaaca aacattcctg ccgagatcat
     3181 cgtggtggac aacggcagca ccgatcgaac ggccgaacgc gcgcgagacg cgggcgcgcg
     3241 cgtggtgagc gaaccgatcc ccggttacgg tcgcgcgtgc atggccggaa tacgcgccct
     3301 ggcgccacaa tgcgacattg tcgtttttct cgacggcgat ggaagtgatt gccccgagtt
     3361 catgaatcaa cttgttgatc ccatcgcgag cggcacgcac gatttcgtga tcggctcgcg
     3421 cacgcgcggg gagcgcgaac cggggagcat gaatttccag cagatcgttg ccggacgaat
     3481 ggctggcctg attctgcgat tgctctatgg agttcgtttt accgacatgt gcccgtttcg
     3541 cgcgatccgg cacgatgcgc ttgaaaagct cggtatgcgc gagcaaacct atggctggaa
     3601 tctcgagatg cagatgaaat ccgcgcgcgt tgggctgcga atcctcgaaa ttcctgtaaa
     3661 ccatcgtcga cgcgctggtg gggaatcgaa agtttcgggc acattgcgcg ggacatttat
     3721 cgccggcgcg cgcattatcg cgacgctctt gcgcatcgcg acagaaagga agccacggat
     3781 gaacactgat tagcacggat gtttgaagag atgacacgcg agagactctt cttctgatcc
     3841 gtgtttcatc tgtgttaatc tgtggccgaa taataattca tgacattgcc tgacgaactt
     3901 tcgcgcggga aggcaatcgc caccggtttg cttgccggcc tcgtggcggc aatcgccatg
     3961 accgtcatga tgttgttgct cgcgtgtttc ggtgtagcga caccgctggt gatcattggc
     4021 gatcgccttt cggttttcat tccgccgggg ccatttcttg cgctcatggg aaaggtcggc
     4081 ggctacaatc atctgaaaca gctcggggtt ggatcgacga tcgcgggcca gcttattgtt
     4141 ggcgcgattg gcggagcgat ttttggattt tgcatgcggc gcaaccctgg aaagccagcg
     4201 acgctggcaa cgatcgccgt ctttattgcg ctgccgctca tcgggctcgt gatcgcactt
     4261 tggccggtgc ttggaacgag ctatgttgga ttgcccattg ctgccgcgcg cctggttaca
     4321 gtcgtcggct tc
//
LOCUS       DASQUQ010000074        13583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_15420, whole genome shotgun sequence.
ACCESSION   DASQUQ010000074 DASQUQ010000000
VERSION     DASQUQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15420"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /gene="purS"
                     /locus_tag="VE486_02075"
     CDS             complement(<1..224)
                     /gene="purS"
                     /locus_tag="VE486_02075"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HZA37905.1"
                     /translation="MKVKVIVMPKAAVLDPQGNAVRDAMQHLGMPEVRSVRIGKYMEI
                     DVDGQNGDLEARLHGLCRDLLSNPVIEDYEL"
     gene            complement(290..2026)
                     /gene="ligD"
                     /locus_tag="VE486_02080"
     CDS             complement(290..2026)
                     /gene="ligD"
                     /locus_tag="VE486_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZA37906.1"
                     /translation="MGLADYKAKRDFRKTAEPKGGKPLPKAVRGASRFVIQKHDARRL
                     HYDFRLEMEGVLKSWAIPKGLPWKKGEKHLAVEVEDHPIDYEAFEGVIPEGNYGGGTV
                     MVWDSGAYYVYGEQPAKSLREGKLHLILDGKKAKGEWTLVRIRGRDGEKNQWLILKTG
                     ADAKLISKKLEDQSVKTGRTMKQIAEARDAEWESNREEKDGSAASQFKARIRTAIKKK
                     EKGGAKDEIVGQVHRLPNTKPGNRGDRLAIRSLSDLPSAKARFIEPMKARLVENPPTT
                     GDWIYELKFDGIRLIVIKTGKKVSLLSRNENELGGRFPEIVEAVKNLPARECVIDGEV
                     VALDEEGRSSFQLLQAREMEGMKSLVYFYAFDLLQLDGKSLISFPLEARKSVLKQLCA
                     GAGDPIRYSSNIGGDAKVLLDEVKRRGLEGIIGKQRNSIYEPGRRSGAWIKLKCVREQ
                     EFVIGGYTPPQGSRKHFGAILVGYYKDKTLVFAGKVGTGFTAKSLSMFYKKFRVQERS
                     DCPFVDLPSKQNGQWVLGITPSMMRKMHWVNPVFVCEIKFAEWTRDKKLRAPVFLGLR
                     EDKKPSDVIREV"
     gene            complement(2100..2705)
                     /gene="tsf"
                     /locus_tag="VE486_02085"
     CDS             complement(2100..2705)
                     /gene="tsf"
                     /locus_tag="VE486_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZA37907.1"
                     /translation="MEIGPQLVKQLREKTNAGFMDCKRALAESGGDLEKAEAILRMKG
                     IAGATKKASRATKEGIVASYIHLQGKVGVLVEVNCETDFVARNENFREFVKDITLHIA
                     AAHPLYVGRADVPGNLIEAEREIYKAQVKGKPENVMSKIVEGKLDKFFSTVCLLEQAF
                     IKNPDQTIEDLVKSKITELGENIVIRRFTRYLMGEPLPAEA"
     gene            complement(2759..3490)
                     /gene="rpsB"
                     /locus_tag="VE486_02090"
     CDS             complement(2759..3490)
                     /gene="rpsB"
                     /locus_tag="VE486_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZA37908.1"
                     /translation="MAETTELVPELLEAGVHFGHQTKRWNPKMKPFIFEQRNQIYIIN
                     LDETVVQLRRATEFLQEVIRRGGKILFVGCKKQAQEAIKEAALACGQFYVNQRWLGGT
                     LTNLSTIRKSIGRLKYIEQIEQSPEYKKMGKQELAALNREAAKLRRNLDGILEMSQLP
                     DALVVIDTIREQNAVNEARRLGIPVVAIVDTNADPDLIDYPIAGNDDAIRAIRVILQK
                     LVDATVSAGGEARIREQVEMAGVSA"
     gene            complement(3578..4120)
                     /locus_tag="VE486_02095"
     CDS             complement(3578..4120)
                     /locus_tag="VE486_02095"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZA37909.1"
                     /translation="MSIVTKTGDKGETSLMYGRRVSKADPRVDAYGCVDELTAALGLA
                     RSISNEKFLSDQILAAQRDLIVVMGELATTPQDRERYIKDDFHLTSAAMVDRITAVIV
                     DLEKDKSLYPKDWVIPGETTTSAALDFARATCRRAERHIAGFTGDDKDFNPEILRYLN
                     RLSDLLWVLARYAERRSRAK"
     gene            complement(4216..8340)
                     /gene="rpoC"
                     /locus_tag="VE486_02100"
     CDS             complement(4216..8340)
                     /gene="rpoC"
                     /locus_tag="VE486_02100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZA37910.1"
                     /translation="MNEHSWRELVGEERPVGFDQVAIGVASSDTMRSWSKGEVKNPET
                     INYRTFKPEKGGLFCERIFGPTRDWECSCGKYKRIKHKGVICDRCGVEVTLARVRRER
                     MGHIELAVPVSHIWFYKCMPSRIGLMLDMSARQLERVIYYEDYIVIDPGKTPLQKAQL
                     LNEVEYREAQEQYGDDFVAGMGAEAVKKLLAEIDLNKLNKELEKAMGATKSKQIRKKL
                     AKRLKLVQGFQNSHARPEWMILDVLPVIPPDLRPLVPLEGGRFATSDLNDLYRRVINR
                     NNRLKNLLLLKTPDVIIRNEKRMLQEAVDALFDNGRHGRAVTGAGNRPLKSLSDMLKG
                     KGGRFRQNLLGKRVDYSGRSVIVIGPELKLHQCGLPKKMALVLFEPFIIRRLKDLGYV
                     HTVRSAKKMIERQTPEVWDILDEVTKGHPVLLNRAPTLHRLSIQAFEPQLIEGEAIRI
                     HPLVCTAYNADFDGDQMAVHVPLSVEAQMEARMLMLAPNNIFSPSSGKPITTPSQDIT
                     LGCYYLTQNPRRAAGSDGDGRLPLFSDPAEIEFAMAEGSVRTHDRIRIKNPDFGNQTI
                     YGNAEAKTIETTAGRVIFNQIWPKQLGFFNKAAGKKQLSDIIWRCYQIAGPAETVATL
                     DKLKELGFAEATKAGISIGISDMIIPKEKETELENAYKQIRQVEQQYRKGIITDGERY
                     NKIIDIWTHAGDEISSVMFRTLEHNEGRKELNPVYLMVDSGSRGNRQQVRQLAGMRGL
                     MAKPSGEIIERPITSNFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRKLVDAA
                     QDVIINEEDCGTVNGIVVRSIYEGDEEVVDLGQRIIGRVSCETIADPVEKKKKIVKAN
                     QLIDEKIAAELQRIGVESLKIRSVLTCECGRGVCAMCYGRNLATGQFVKLGEAVGIIA
                     AQSIGEPGTQLTMRTFHIGGTASQTFKQPIIKAKNDGTVQFNELRTVQALDGSWIVLS
                     KNGSISVHNAEGRELERYNVVIGSMISKDDGASVKKGETFVQWDPYNVPILTDKSGKI
                     EFRDMIGGVTIKRDVDEATGLMGTVIIEHKEDLHPQILIVGDKKELLASYSIPAGAHV
                     IVEEGQKVRAGALLAKTPRKVAKTKDITGGLPRVAELFEARRPKDAAEIAKIDGVVEM
                     GGTARGKRRLILKDPDTGTEEEHLIPLTKHIIVFKGDFVKKGQQLTEGPVVPHEILEV
                     CGPQDLQEHLVNEVQEVYRLQGVEINDKHIEIIVRQMLRKVKITDPGDTSLLWGDQVD
                     RIDFEAENQRVVEQGGKPAEAVPVLLGITKASLETDSFISAASFQDTTRVLTEAATLG
                     KVDRLRGFKENVIMGHLIPAGTGFPQHREIKLVELGEQIGAPVMEEAEPQPAIG"
     gene            complement(8370..12167)
                     /gene="rpoB"
                     /locus_tag="VE486_02105"
     CDS             complement(8370..12167)
                     /gene="rpoB"
                     /locus_tag="VE486_02105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZA37911.1"
                     /translation="MSERIYFGSIKEGIEPPNLIEVQLNSYIDFLQKDVPFSKRKNQG
                     LQAVFKEVFPIESYDEKAVLDFSHYEIGEPKLSALEAQREGQTYSAPLHVTFQLREEK
                     GTKEEKVYMGEIPLMTPQGTFVINGAERVVVSQLHRSPGIAFESTVHVNGKVLYSFRI
                     IPDRGSWIEVQFDTADLLYIYLDRRKRRRKFLATTFLRALGYGSDEEVIKLFYNIENL
                     KLSEKLDEEQLATKVLTAEVRDGEVTVARAFEPLTLATVRQIMALKIHEVKVIDISND
                     DTVVKALRKDPAHDQEEALKDIYRRLRPGDPPTVANARSLLKRLFFDPKKYDLGRVGR
                     YKINQKLGINVDSSERILTDKDFIAAIKYLINLRRGEGTLDDIDHLGSRRVRTVGELL
                     ANQCRIGLARTERLVKERMTLFDINVDGMTPQKLINPKALSAVIRDFFGRSQLSQFMD
                     QTNPLAELTHKRRLSALGPGGLSRERAGFEVRDVHPSHYGRICPIETPEGPNIGLISS
                     MSTFARINEFGFIETPYRKVENGRVTDQIDYLTGDREENFLVAQANAPIDGRGRFTGE
                     KVSVRYRGDFLEVDPSKVHYMDVSPKQLVSVAAGLIPFLEHDDANRALMGSNMQRQGV
                     PLIVSEAPLVGTGLEEKVARDSHAVVLATESGKVASVTADQIIVTKDGHLPEGKKKLK
                     TDPEEGIHIYELRKFMRSNAGTCVNQKPIVRKGQHVKRGQIIADGPNTDHGELALGRN
                     VLVAFMPWNGYNFEDAIMISEKVVKEDIYTSIHIDEFEIGARDTKLGPEEITRDIPNV
                     SEEALRNLGPDGVVRVGAEVKPGDILVGKITPKSETELAPEERLLRAIFGEKAADVKD
                     TSLTVPSGTYGIVMDVKVSSRREISREKLTPAETKRQLKTIGEEHRRKREELTEQLTD
                     SLSNILLGEKIPLDVVNAETGEIIIPANRKITKTLLRKLAIVHDHIEIDPSPIRNKIR
                     EIIGSYEHKFAELELERERAVDRVESGDDIDPGIIKQVKVYISSKRKLSVGDKMAGRH
                     GNKGVVARIVPEEDMPFLADGTPVEIVLNPLGVPSRMNVGQVLETHLGVAAKALGFKV
                     ATPVFDGIKEKQIREYLQDAKKKEGFSWVQANGKARLFDGRTGDPFDQEVVVGHIYMM
                     KLGHLVADKIHARAVGPYSLVTQQPLGGKAQYGGQRFGEMEVWALEAYGAAYTLQELL
                     TVKSDDVQGRTRIYESIVKGDNSLEAGTPESFNVLIKEMQSLGLDVKVGGQAPSSFLE
                     NVA"
     gene            complement(12474..12866)
                     /gene="rplL"
                     /locus_tag="VE486_02110"
     CDS             complement(12474..12866)
                     /gene="rplL"
                     /locus_tag="VE486_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZA37912.1"
                     /translation="MADTNKLVEQLSELSVLEIAGLVKQLEEKWGVSAAAPVAVAAGP
                     AAGPGAGAAAAPAEEKSTFDVILKEMGSNKIAVIKEVRGAVPGLGLADAKALVEGAPK
                     TIKEGVPKEEAEEIKKKIEAAGAKVEIK"
     gene            complement(12980..13513)
                     /gene="rplJ"
                     /locus_tag="VE486_02115"
     CDS             complement(12980..13513)
                     /gene="rplJ"
                     /locus_tag="VE486_02115"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZA37913.1"
                     /translation="MRPEKTSIVSDLSEKLKRSPFVLVTDYQHMKVGDFGELRNRLAP
                     TGAEVHVVKNNFLKRAMTNSGFPDVGDKLVGQTAVVTGEKDVAPVAKIFKSFATEFKI
                     AALKIGFVDRAVLSTAELETLADLPPREVLQAQLLGFLLAPATMLVRLLNEPASSLAR
                     LLKAKSEKEGASAQAAA"
BASE COUNT         2752 a         3933 c         3487 g         3411 t
ORIGIN      
        1 agctcatagt cttcaatcac cggattcgac aacaaatcgc ggcaaagccc gtgcaatcgc
       61 gcttcaagat cgccattctg cccatcgaca tcgatctcca tgtatttgcc gatcctgacg
      121 ctgcgcacct ccggcatgcc caggtgctgc atcgcgtcac ggaccgcgtt accctgcgga
      181 tcgagcactg ccgccttcgg catcacgatg accttcactt tcatcgcctc accatatttg
      241 cagcttagac gcgaacaagt gcaatccggg aatctttctg tcagtggcgc tacacctctc
      301 gaataacgtc gctgggcttt ttatcttcgc gtaatccgag gaagaccggc gcgcgcaatt
      361 tcttatctcg agtccattcg gcgaacttga tttcgcagac gaataccgga tttacccaat
      421 gcatcttgcg catcatggaa ggcgtgattc ccagtaccca ttgcccattt tgcttcgatg
      481 gtaaatcgac aaatgggcag tcgctacgtt cttggacccg gaatttcttg tagaacatcg
      541 ataatgattt cgcagtgaag ccggttccca ctttgccggc aaaaacgagc gttttgtcct
      601 tgtaataacc aaccaggatt gcaccaaaat gtttgcgcga tccttgcggc ggtgtgtatc
      661 cgccgatgac aaactcctgt tcgcgcacgc atttgagctt gatccatgcg ccgcttctac
      721 gcccaggctc gtaaattgaa ttgcgctgtt tgccgatgat cccttccagc ccgcgacgtt
      781 tcacttcgtc cagcaatacc tttgcgtctc cgccgatatt gctcgagtaa cggatcgggt
      841 cgccagcgcc ggcgcaaagt tgtttcaaaa cactttttcg agcctccaac gggaacgaaa
      901 tcaagctttt gccgtcaagt tgaagcaagt cgaacgcgta gaagtaaacg agagatttca
      961 ttccctccat ctcacgcgct tgcaggagct gaaacgagga gcggccttcc tcgtcgagcg
     1021 caaccacttc gccgtcgatc acgcattcgc gtgccggcaa attctttacc gcctcgacaa
     1081 tctcagggaa gcgtccgcct aattcgttct cattacgcga cagcaacgac acttttttgc
     1141 cggtcttgat cacgatcaga cgaataccat cgaacttgag ttcgtaaatc caatcgcccg
     1201 tagtcggcgg gttctccacc agccttgctt tcattggttc gatgaagcgc gctttggctg
     1261 aaggcaaatc ggacaacgac ctgattgcaa gacgatcgcc ccggttgccg ggttttgtgt
     1321 tcggcaggcg atgaacctgt cctacaattt catcttttgc gccgcctttt tctttttttt
     1381 taatcgccgt tctgatgcgc gctttaaatt gcgacgccgc actcccatct ttttcctcgc
     1441 gattggattc ccattccgca tcgcgagctt ccgcgatttg tttcatcgtg cgtccggtct
     1501 tgaccgactg gtcttcgagt tttttcgaaa tcagtttggc atccgcgccg gttttcaaga
     1561 tcagccactg attcttttcg ccgtcgcggc cacggatgcg cacgagcgtc cattcgcctt
     1621 ttgccttttt tccgtcgagg atgagatgaa gtttgccttc ccgcaacgac ttcgcaggtt
     1681 gctctccgta aacgtaatag gcgccgctgt cccaaaccat caccgtcccg ccaccgtagt
     1741 tcccttcggg aatgacgcct tcgaaggctt cgtaatcgat tgggtgatct tccacttcca
     1801 ctgccagatg tttttcgcct ttcttccaag ggagcccttt cgggatcgcc caggatttca
     1861 aaacgccttc catctctagc cggaaatcgt aatgcagccg gcgggcatcg tgtttctgga
     1921 tgacaaagcg ggatgcaccc cgcaccgctt taggcaaagg tttgccgccc ttcggttcgg
     1981 ccgtttttcg aaaatcgcgc ttcgccttgt aatcggccag acccattggc gttcaattta
     2041 gcgacggtca ttgaccgccg ctacaggaaa gcgccccaac ggaggcaatt attttattcc
     2101 tacgcttcgg caggcagcgg ctcgcccatg aggtaacggg tgaagcgccg gatcacgatg
     2161 ttctcgccca gttcagtgat cttcgatttc actaaatcct caatcgtctg gtctgggttc
     2221 ttaatgaaag cttgctccag caggcagacg gtgctgaaaa atttgtcgag cttgccttcg
     2281 acaattttgc tcatgacgtt ttccggtttg cctttgacct gcgctttgta aatctcgcgc
     2341 tcggcttcga tgagattgcc cggaacgtct gcgcggccta cgtaaagcgg atgcgcggca
     2401 gcgatgtgca gggtgatgtc cttcacgaac tcgcgaaaat tttcattcct ggccacaaag
     2461 tccgtctcgc aattcacttc cacaaggacg ccgaccttgc cttgcaggtg gatgtaagaa
     2521 gccacgatac cctccttggt agcgcgcgat gcctttttgg ttgcgccggc gatgcctttc
     2581 attcgtaaaa tggcctcggc tttttcaaga tcaccgccgc tctccgcaag cgcgcgcttg
     2641 caatccatga agccagcgtt ggtcttttcg cggagttgct tgactagttg tgggccgatt
     2701 tccatggatt cgtaaatcgt tgaaccgtta aagcggattc cggttcgatc gactaatgtc
     2761 aggcggacac gcccgccatt tcgacttgct cacggattcg agcttcgccg cctgccgaaa
     2821 cagtggcgtc cacgagcttt tgtaaaatca cccgaatagc ccgaatggca tcatcatttc
     2881 cggcgattgg atagtcgatc agatccggat ctgcatttgt atcgacaatg gcgacaaccg
     2941 gaatgccgag acggcgcgcc tcattaaccg cgttttgctc gcgaatcgtg tcgatgacca
     3001 ccagcgcatc cggcaactgc gacatttcga gaatcccatc gagattgcgc cggagctttg
     3061 ccgcttctcg attaagcgcg gccagttctt gtttgcccat ctttttgtat tcgggcgatt
     3121 gctcgatctg ctcgatgtat ttcaaacggc cgatgctctt gcgaatggtc gaaagattcg
     3181 tcagcgttcc gccgagccag cgctggttga cgtaaaactg gccgcaagcc agggccgctt
     3241 cctttatcgc ttcctgggcc tgtttcttgc agcccacgaa caaaatcttc cctccgcggc
     3301 ggatcacttc ctgaagaaac tccgtggcgc gccgaagctg gacgacggtc tcatccaaat
     3361 tgatgatgta aatttgattc cgctgctcga aaatgaacgg tttcatcttc gggttccagc
     3421 gcttggtctg gtgcccaaaa tggacacccg cttccaggag ttccggcact agttctgttg
     3481 tctcagccat aaaaaaatca cgccggccga ggggcacggc gagtggcgaa tatgctacga
     3541 cgcctcgcag atcgcaaatt caatttccac ctggtcgtca tttcgctcgc gacctgcgct
     3601 ctgcatatcg cgccagaacc cagaggagat cggagaggcg gttcagatac cgcaaaatct
     3661 ccgggttaaa atctttatca tcgccggtga atccagcgat atgccgttcg gcgcgacggc
     3721 aggttgcccg ggcgaaatcg agagcggctg aagtggttgt ttccccgggg atgacccaat
     3781 ctttcggata aagcgattta tccttctcaa gatcgactat taccgccgtg atccggtcga
     3841 ccatcgcagc gctagtgagg tgaaaatcat ctttgatgta ccgttcccga tcttgcggcg
     3901 tcgttgccaa ttcgcccatc acgacgatca aatctctttg cgcggccagg atttggtcgg
     3961 aaagaaactt ctcgtttgaa atggatcggg caagaccaag cgctgcggtc aactcatcga
     4021 cgcatccata ggcgtcaacg cgcggatcgg cttttgatac gcgccgcccg tacattaacg
     4081 aggtctcgcc cttgtcgccg gtcttggtta cgatcgacat cgcttctact ggtagggcga
     4141 cgctctgcgg agccgacgaa tatttcccag aacggccatc acggctcgac agagtctcgc
     4201 cctaccaaga aatcatcacc cgatggctgg ctgtggctcg gcttcttcca ttacaggagc
     4261 gccgatctgc tcaccgagtt caactaattt gatttcgcgg tgctgaggga atcccgtgcc
     4321 cgctggaatc agatgaccca tgatgacgtt ctctttgaat ccacggagcc gatcgacttt
     4381 gccaagcgtg gctgcttccg taagcacgcg ggttgtatcc tggaacgacg cggccgagat
     4441 aaagctgtcc gtttcgagcg aagctttggt gatgccgagc aataccggca cggcttcggc
     4501 tggcttaccg ccttgctcga ccacgcgctg attctccgct tcaaagtcga tccgatcgac
     4561 ttgatcgccc cagagcaacg aagtgtcgcc gggatcggtg attttcactt tgcgcaacat
     4621 ttggcgcacg atgatttcga tgtgcttatc gttgatctcc acgccttgca gccgataaac
     4681 ctcctggact tcgttcacca aatgttcctg gagatcctga ggtccgcaca cttctaggat
     4741 ttcgtgagga acaacagggc cttccgtaag ttgctggcct ttcttcacga aatcgccttt
     4801 gaagacgatg atgtgtttgg tgagcgggat cagatgctct tcctcggtcc cggtatcggg
     4861 atccttcaaa atcaggcgtc gctttccacg ggcagttcca cccatctcaa cgaccccatc
     4921 aatcttcgca atctctgccg cgtccttcgg ccggcgagct tcgaataatt cggccacgcg
     4981 cggcagaccg ccggtgatgt ccttggtttt tgcgacttta cgcggtgttt ttgccagaag
     5041 ggcgcctgcg cgcaccttct gcccttcttc gacgatgacg tgcgcgccag cggggatcga
     5101 gtaactcgcg agcagttcct tcttgtcacc cacaatgaga atctgcggat gcaaatcctc
     5161 cttgtgctcg ataatcacgg tgcccatgag gccggtcgct tcatcgacgt cacgtttgat
     5221 cgtcacaccg ccgatcatgt cgcggaattc gatcttgcca ctcttatcgg tgagaatcgg
     5281 gacgttatag ggatcccatt gcacgaaggt ctcacctttc ttgactgagg cgccgtcgtc
     5341 tttagagatc attgaaccga tcaccacgtt gtaacgctcc agttcgcggc cctccgcgtt
     5401 atgcacgctg atcgagccgt tcttactcaa aacgatccag ctgccgtcga gcgcctgcac
     5461 cgttcgcagc tcgttgaact gaaccgtgcc atcgttcttc gccttgatga tcggttgctt
     5521 gaaagtctga ctggcagtgc cgccgatatg gaatgtccgc atcgtcaact gcgttcccgg
     5581 ctcgccgatc gattgcgctg caataatgcc gacggcttcg cccagcttca cgaattggcc
     5641 cgtcgcgaga ttgcggccat agcacattgc gcaaacgcca cgcccacatt cgcacgtaag
     5701 caccgaacga atctttaagc tctcgacgcc aattcgctga agctccgccg cgattttctc
     5761 atcgatgagc tggtttgcct tgacgatttt cttcttcttc tcgaccgggt ctgcgattgt
     5821 ctcacaactg acgcgaccaa taatgcgctg gccgagatcg acaacctcct catcgccctc
     5881 gtaaatcgag cgcacgacga ttccatttac cgtgccgcaa tcttcttcgt tgatgatgac
     5941 gtcctgcgcc gcgtctacca atttgcgggt aagataaccg gagtccgcgg ttttgagcgc
     6001 agtgtccgcg agacccttac gcgctccgtg cgtggaaatg aagtattcga gcacggtcag
     6061 cccctcgcgg aagttggacg tgatcgggcg ctcgataatt tcgccgcttg gtttggccat
     6121 caaaccgcgc atgccagcga gctgccgtac ctgctgacgg ttgccgcgtg agcctgaatc
     6181 gaccatcagg tagacaggat tcagttcttt gcggccctcg ttgtgttcga gggtgcggaa
     6241 catcacgctg gaaatttcgt ctccggcgtg cgtccagatg tcgatgatct tgttgtaccg
     6301 ttcaccatcg gtaatgatgc ctttgcgata ttgctgctcg acctgccgga tttgcttgta
     6361 agcattttcg agctcggttt ccttctcctt cggaatgatc atgtccgaga ttccaatcga
     6421 gatgccggct ttggttgctt cggcaaaacc gagctccttg agtttgtcga gggttgcgac
     6481 cgtttcagcc ggtcccgcga tctgataaca gcgccaaatg atgtcgctca gttgtttctt
     6541 gcccgccgct ttgttgaaaa atccgagctg cttcggccaa atctggttga aaatgacacg
     6601 gcccgccgtg gtctcgatcg tcttagcctc cgcgtttcca taaattgttt ggttgccaaa
     6661 atcggggttc ttaatcctga tgcggtcgtg cgtccgaaca ctcccttcgg ccatggcaaa
     6721 ctcgatctca gccggatcgg aaaatagcgg gagacgcccg tccccatcac ttccggcggc
     6781 tcgccgcgga ttttgggtga ggtaataaca gccgagtgtg atgtcctggg agggagtcgt
     6841 aatcggctta ccactggaag gagagaagat gttgttcggg gccaacatca gcatgcgcgc
     6901 ttccatttgc gcctccaccg aaagcggcac atgcacggcc atctggtcgc cgtcaaagtc
     6961 ggcattgtaa gcggtacaaa ccagcggatg gatgcgaatc gcttcgcctt cgatcaattg
     7021 cggctcaaaa gcctggatcg acaatcgatg caaggtcggc gcgcgattga gcaataccgg
     7081 gtgacctttc gttacctcgt ccaagatgtc ccacacttcc ggcgtctgcc gctcgatcat
     7141 ctttttcgcg ctgcgcacgg tatggacata accaagatct ttcagccggc ggatgatgaa
     7201 cggctcgaaa agaacgagcg ccatcttctt aggcagaccg cattgatgaa gtttcagttc
     7261 gggaccgatg acaattaccg agcggcctga ataatcgacg cgcttgccga gcaggttctg
     7321 gcggaaacgt ccacctttgc ctttgagcat gtcgctcagc gacttgagcg gccgatttcc
     7381 cgcgccggtg accgcgcgac catgccgtcc attgtcgaac aacgcgtcca cggcttcctg
     7441 tagcattcgc ttttcgttgc gaatgatgac gtccggggtt ttgagcagaa gcaggttttt
     7501 gagccggttg ttgcgattaa taacgcgacg atacagatcg ttcaggtccg aagttgcgaa
     7561 acgcccgcct tcgagcggaa cgagcggccg caagtccggc gggatcaccg gtagcacatc
     7621 gaggatcatc cattcgggtc gcgcgtgtga attctggaag ccttgaacca gtttcagccg
     7681 tttcgcgagc ttcttacgaa tctgtttgct cttggtggcg cccatcgctt tttcgagctc
     7741 tttgttgagt ttgttgaggt cgatctcggc gagaagcttt ttgaccgctt ccgcgcccat
     7801 gccggcgacg aaatcgtcac catactgctc ctgcgcttca cggtattcga cttcgttaag
     7861 cagttgtgct ttttgcaaag gggttttgcc cggatcgatg acaatgtagt cctcgtaata
     7921 aatgacgcgc tcaagctgac gcgcgctcat atcgagcatc agcccgatgc gcgagggcat
     7981 gcatttgtag aaccagatgt gtgaaactgg gacggccaac tcgatatgac ccatgcgttc
     8041 gcgccggacg cgtgccaacg tcacttcaac cccgcaccgg tcgcagatga cgcccttgtg
     8101 tttaatgcgt ttgtattttc cgcaggagca ttcccaatct cttgtcggac caaaaattcg
     8161 ttcgcagaaa aggccgccct tctccggttt aaaagtgcgg tagttgattg tttccggatt
     8221 tttgacttcc cctttgctcc acgagcgcat cgtgtcgctg gacgcgacac cgatcgcgac
     8281 ctgatcaaaa cccacggggc gttcttcgcc cactaattca cgccaggaat gttcgttcat
     8341 gattattgaa gtaatttgtc gatgtaaatt tacgcgacgt tctccagaaa actgctgggc
     8401 gcctggccgc cgactttcac gtcgagaccg aggctctgca tttccttgat aagcacgttg
     8461 aaagattcag gggtacccgc ttcgagtgag ttgtcgccct tgacgatcga ttcatagatg
     8521 cgggtccggc cctgcacgtc gtccgacttt acggtaagca gctcctgcaa cgtgtaggcg
     8581 gcaccgtatg cctccagggc ccacacttcc atttcgccga agcgttgtcc gccgtattgc
     8641 gccttgcccc cgagcggctg ttgcgtgacg agcgagtacg gtcccactgc gcgcgcgtga
     8701 attttgtcag ccaccaggtg gccgagcttc atcatataaa tatggccaac cacgacttcc
     8761 tgatcgaaag gatcgccggt tcgtccatca aaaagccgcg ctttaccgtt ggcctgcacc
     8821 caggagaaac cctccttctt ctttgcgtcc tgcagatatt cacggatctg tttttctttg
     8881 attccgtcga agaccggggt cgccactttg aatccgagcg ccttcgcggc aactcccaga
     8941 tgggtttcca agacctggcc gacgttcatg cggctcggca cgcccagcgg attgagtacg
     9001 atctcaaccg gcgtcccatc cgccaggaag ggcatgtctt cttcaggaac gatgcgagca
     9061 acgacgccct tgttcccgtg acggccagcc atcttatcgc cgacgctcaa cttgcgcttg
     9121 ctcgaaatat aaaccttaac ctgcttgatg atccccggat cgatatcatc gccgctttct
     9181 actctgtcta cggcgcgctc gcgttcgagc tccagttccg cgaacttgtg ttcatacgaa
     9241 ccgatgatct cgcggatttt gttacggatt gggctgggat caatctcgat gtgatcgtgc
     9301 acgatcgcga gctttcgcaa taatgttttg gtaatcttgc gattcgccgg aatgatgatt
     9361 tcgccggtct cggcatttac gacgtcgaga gggatttttt cgccgagcag gatattcgaa
     9421 agcgaatcgg tgagttgttc agtcaactcc tctctcttgc gcctgtgctc ttcaccgatc
     9481 gttttgagct gtcgtttggt ttctgcgggg gtgagttttt cgcgcgagat ttcgcgacgg
     9541 gacgagactt ttacgtccat cacgattcca taagtgcccg aaggaacggt aagcgaagtg
     9601 tccttcacgt cggctgcttt ttcgccaaaa atagcgcgga gcaatcgttc ttccggcgcc
     9661 aattcggtct cgctcttggg cgtgattttg cccaccagga tgtcgcccgg cttcacttcc
     9721 gcaccgactc gcactacacc atcgggaccc agattgcgta aggcttcttc actgacattt
     9781 ggaatgtcgc gcgtgatttc ttctggtcca agcttcgtat cgcgggcacc gatctcgaat
     9841 tcatcgatgt ggatggaggt ataaatatcc tccttcacca ctttctcaga gatcataatg
     9901 gcatcttcaa agttgtaccc gttccaaggc atgaacgcga cgagcacatt gcggcccagg
     9961 gcgagttcgc catgatcagt gtttggaccg tcggcgatga tttgaccgcg ttttacatgc
    10021 tggcctttac gcacaatcgg tttctgattt acgcatgtgc cagcatttga gcgcataaat
    10081 ttgcgcaact cgtaaatgtg aattccctct tcggggtccg tcttcagctt ttttttgcct
    10141 tcgggcagat gcccgtcctt cgtaacaatg atctgatcag ccgttaccga ggccaccttg
    10201 ccactttcgg tcgcgaggac aacggcatgg gaatcgcgcg ctactttctc ctccaagcca
    10261 gtgccaacaa gcggcgcctc ggaaacaatg agcggaacgc cctggcgctg catgttcgaa
    10321 cccatcagcg ctcggtttgc gtcgtcgtgt tcgagaaatg gaatcaggcc cgctgccacc
    10381 gataccagtt gcttgggcga cacatccatg tagtgcacct tcgagggatc gacctcaaga
    10441 aagtcgccgc gatatcgcac cgatactttt tctccagtga aacgcccgcg tccgtcaatt
    10501 ggcgcgttcg cctgcgcgac gaggaagttt tcttcgcggt cgccggtaag atagtcaatt
    10561 tgatccgtca cccggccgtt ttctaccttg cgatacggcg tctcgataaa gccgaattca
    10621 ttgatgcgcg cgaacgtgct cattgaactg atcaggccga tgtttggacc ttcaggcgtc
    10681 tcaatcgggc aaatgcggcc gtaatgcgac ggatgaacgt cgcgcacctc gaatccggcg
    10741 cgttcacggc tcaagcctcc aggcccaagc gccgacaaac gccgcttgtg cgttaactcc
    10801 gccaacggat tggtttggtc catgaattgt gaaagctgcg agcgaccaaa gaaatcgcgg
    10861 atgactgcgc tgagcgcctt cggattgatc agcttttgcg gtgtcatccc atccacattg
    10921 atgtcgaaca gcgtcatgcg ctctttaacc aggcgttcgg tgcgagcaag accaatacgg
    10981 cactgattcg ccagcagttc gccgaccgtg cggacgcggc ggctgcccag gtgatcgata
    11041 tcgtcgagtg tcccctcgcc ccggcgcaag ttgatcaaat acttgattgc cgcgatgaaa
    11101 tccttatccg tcaaaatacg ctcactggaa tcgacgttga tcccgagttt ctgattgatt
    11161 ttatagcggc cgacacgtcc cagatcgtat ttctttggat cgaaaaagag ccgttttagt
    11221 aaagagcgcg catttgccac ggtgggggga tcgcccgggc gcaaacgccg gtaaatgtct
    11281 ttgagcgctt cctcctggtc gtgcgccgga tccttcctca gagccttgac gactgtgtcg
    11341 tcgttcgaaa tgtcgatcac ctttacctcg tgaattttaa gcgccatgat ctggcgaacg
    11401 gtcgccagag tgagaggctc gaacgcgcgc gccaccgtta cttcaccatc acggacttcg
    11461 gctgtgagaa ctttggtggc cagctgctct tcgtcgagct tttcgctcag cttcagattt
    11521 tcgatattgt aaaagagctt gatgacttct tcgtccgagc cgtaaccgag cgcgcgcaga
    11581 aacgttgtgg cgagaaactt gcggcggcgc ttgcgtcgat cgagataaat gtaaagaaga
    11641 tcggccgtat cgaattgcac ctcgatccag gaaccgcggt ccggaatgat gcggaagctg
    11701 tagaggacct tgccgttgac gtgaacggtg gactcaaaag cgattcctgg cgaacggtgt
    11761 aactggctca cgaccacacg ctcggcgcca ttaatgacga atgtgccttg gggagtcatg
    11821 agcgggattt cgcccatgta aactttctct tccttggtcc ctttttcctc gcgtagttgg
    11881 aaggtgacgt gcaacggcgc gctgtaagtc tggccttcgc gctgcgcctc cagcgcggac
    11941 aatttcggct cgccgatttc gtagtgactg aagtcgagca ccgctttttc gtcgtagctc
    12001 tcgatcggaa agacctcctt gaacacagcc tggagcccct gattcttccg cttggaaaac
    12061 gggacgtctt tctggagaaa atcgatgtat gaattgagct gaacctcaat cagattcggt
    12121 ggttcgatgc cttccttaat gcttccgaaa taaatgcgtt ccgacataat cactagacca
    12181 agcgttgacc tcgcaacggc cgcaccaaat cgcgtccgac gcgggtgaga ttttacggga
    12241 tcagcgacga gcgagaggcg caaaaatggc accaaaacag cggcctgtca aattcaccac
    12301 cgttttgctg ccacttttgt gttttgccgt cgaacgccgg gttaaaaata cctctgccta
    12361 cttaacagat tcctacccta gatgaaaaca tccgccgcgc aagtaaaatg tttcggcgcc
    12421 ctcgccgaag ccaccaagca cgcgagggcg cgcgcgttcc cccatagcgc gctttattta
    12481 atttcaacct tcgcgcctgc ggcctcgatc ttcttcttaa tctcttccgc ttcttccttt
    12541 ggcacgcctt ccttaatggt cttgggcgct ccttcaacga gcgctttggc atccgccaga
    12601 cccagtccag gcacagcgcc gcgaacttct ttgatcacag caatcttgtt tgagcccatt
    12661 tcctttaaga tcacatcgaa tgtgcttttt tcttcagccg gcgccgctgc cgcgcctgcc
    12721 ccgggtccag ccgcgggacc agctgcaacc gcgaccggag cggctgcgct cacaccccac
    12781 ttttcttcga gttgcttgac caagcctgct atctcgagca cagagagctc gctgagttgt
    12841 tccactaact tatttgtatc tgccattttt atttcctcca attgccgtct ccgaaagcct
    12901 tcgaaaaatt aaggccgata gccatcagcc cggacagttt tgtttcgctt cccgtcccgc
    12961 cattgcggga ggaaaatctt tacgccgccg cttgcgccga agcgccctcc ttttctgact
    13021 tcgctttcag cagccgcgcc agcgacgaag ccggctcgtt gagcagccgc acgagcatcg
    13081 tcgcaggtgc aagcaaaaat ccgagcaatt gcgcctgcaa aacttcgcgt ggcggcaagt
    13141 ccgccagggt ctccaattcg gccgtcgaaa gaacggcgcg atcgacaaaa ccaatcttga
    13201 gtgcggcgat cttaaactcg gttgcaaatg acttaaaaat tttcgcgact ggcgccacgt
    13261 ctttttcccc cgtaacaact gccgtttggc cgaccagctt gtcgccaaca tctggaaaac
    13321 ctgaattcgt catagcgcgc ttgaggaaat tatttttcac cacgtgcact tccgcgcccg
    13381 ttggcgcaag ccgattccgc aattcgccaa aatcgccaac tttcatgtgc tggtaatcgg
    13441 tcacaagcac aaaaggcgag cgtttcaatt tctcggaaag atctgagacg atgctggttt
    13501 tttctggtcg catattagcg gtctaattat tgtcgatcta aagctcagac actcaaatac
    13561 ggcgctggat cgacgcggac tcc
//
LOCUS       DASQUQ010000075         3122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_283293, whole genome shotgun sequence.
ACCESSION   DASQUQ010000075 DASQUQ010000000
VERSION     DASQUQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283293"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1915)
                     /locus_tag="VE486_02120"
     CDS             complement(<1..1915)
                     /locus_tag="VE486_02120"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZA37914.1"
                     /translation="MPAVATGPMIRREIGDDHICLLTFDRPESGANIFDAATLDELNE
                     HLDFVQNDSSLRGLIIASAKKSIFIAGADLKTLLQQAQTGDMRAFIAEGQRVFNRLAE
                     LEIPTVAAIHGACAGGGYEVTLACDYRVASDDPATRIGLPETTLGLIPAWGGCTRLPR
                     LIGAEKAGDVIAQGKLYSAQEASKLGLVDEIASRDQLVDRARQKLRDGKRKPSTAPKI
                     EQEIPAPRDHNPAPARALEVIKKGLFASTQQSLAFELEAIVELGKTKSTQNLIRNFFL
                     AEKYKKGTSKAAVSKVVHAAVIGAGVMGSGIAQWLSSRGVTVILRDIAREQIDRGLAN
                     IEKTYADAVKRGLMTEEKAKEGRARIVASTAPMELRDVQFVIEAASEKIEIKKEIFRE
                     LAMQAGPKTIVATNTSALPVSQLADATVSPEHVIGLHFFNPVSRMKLVEVVVAKQTSD
                     DTRERTLAFARQIGKLPVLVRDSPGFLVNRVLFPYLLDAAELFESGIEADKIDNALVQ
                     WGMPMGPLRLIDEIGVDITIDIGNTLEKAYGRRDHVPAVLLWLRDGEMLGRKTGAGFY
                     KYEGKTQTPSDSLAQWRRALHGEPEGAEGPNIPPEMHRDPRLTLGEDQLTHRLTLLMV
                     NEAARCVEEKIVDS"
     gene            2098..>3122
                     /gene="mgtE"
                     /locus_tag="VE486_02125"
     CDS             2098..>3122
                     /gene="mgtE"
                     /locus_tag="VE486_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZA37915.1"
                     /translation="MVGKILEPEIKSMIDARNFGALREVFRDWAPADVAEVILDMPED
                     EQVIIFRVLPHALAADVFEYLDFDAQQKLLRAMAHEQVVGILNEMAPDDRTELLQELP
                     SAAARQLIRSLTPQERQIAQALLGYPEGSVGRLMTPDFIAVREDWTVQQVLDYIREHG
                     QDSETLNVIYVLNERGKLIDDIRMREFLLKPLSSAVRDLMQKTFVALNVTDSQQDALN
                     VFRKYDRTALPVIDSSGVLLGIVTIDDMLDVAEAEATEDIQKFGGVTALDEPYMRLSL
                     TGMVRKRAPWLVILLLGEMLTASAMAYYEEELAKALVLAVFLPLIIASGGNSGSQAST
                     LMIRAMAL"
BASE COUNT          657 a          915 c          870 g          680 t
ORIGIN      
        1 gcgaatcgac tatcttctct tccacgcaac gcgcggcttc attgaccata aggagggtta
       61 atcggtgcgt aagctgatct tcgcccaacg ttaatcgcgg atcgcgatgc atttccggcg
      121 gaatgttcgg tccctcggca ccttcgggct cgccatgcaa cgcgcgccgc cattgcgcga
      181 gcgagtcgct tggcgtctgc gttttaccct cgtatttgta aaaacccgcg ccggttttgc
      241 ggccaagcat ctcgccgtcg cgcaaccaaa gcaggacagc tggaacatga tcgcgccgcc
      301 cgtaggcttt ctcgagcgta ttgccaatat cgatcgtgat atcgacgcca atttcatcga
      361 tcaatcgcag cggccccatc ggcatgcccc attgcacaag cgcattgtcg atcttgtccg
      421 cctcgatgcc gctctcaaaa agctcagccg catcgagcaa ataaggaaac aagacgcggt
      481 tgacgagaaa acccgggctg tcgcgcacga gaacgggcag cttcccgatt tgccgtgcga
      541 aggccagtgt ccgctcgcgc gtgtcatccg aagtttgttt ggcaacgaca acttccacca
      601 gcttcatccg gctcacagga ttgaaaaaat ggagaccgat gacgtgctcg ggcgatacgg
      661 tcgcatcggc cagctggcta acgggaagag ccgacgtatt cgtggcgacg atcgtcttcg
      721 gaccagcctg catcgctagc tcgcggaaga tttctttttt gatctcgatc ttctccgacg
      781 cggcttcgat tacaaactgc acatcgcgca attccatcgg cgccgtcgat gccacaatcc
      841 gggcgcgccc ttctttcgct ttttcctcag tcatcaaacc gcgcttcaca gcgtcggcgt
      901 aagtcttttc gatattcgcg aggccgcggt cgatttgttc ccgcgcgata tcgcgcagga
      961 tcaccgtaac gccacgcgag ctgagccatt gcgcaatgcc ggaacccatc acgccggcgc
     1021 caatcaccgc ggcatgcacg accttggaca ctgccgcttt ggaagttcct ttcttgtatt
     1081 tctcggccag aaaaaaattg cggatcaggt tttgggtcga tttggtcttg ccaagctcga
     1141 caatcgcctc cagctcaaag gcgagcgatt gctgagttga agcaaaaaga cctttcttaa
     1201 tcacttcgag agcgcgggcc ggcgccgggt tatgatcacg cggcgcggga atttcctgct
     1261 caatttttgg cgccgtcgat ggcttgcgtt tcccgtcccg caacttttgc cgcgcgcgat
     1321 cgacaagttg atcccgcgac gcgatttcat ccaccagacc gagtttcgag gcctcctgcg
     1381 cagaataaag ttttccttgt gcaatcacgt caccggcttt ctccgctcca atcaggcgcg
     1441 ggagccgcgt gcaaccgccc cacgcgggaa tgaggcccag cgtcgtttcg gggagtccga
     1501 tccgtgtcgc cggatcatcc gaggcgaccc gataatcaca cgccagcgtg acctcatagc
     1561 ccccgcccgc gcaagcgcca tggattgccg caaccgtggg gatctccaat tcggcaagcc
     1621 gattgaagac ccgctgtcct tccgcgatga acgcgcgcat gtcgccggtt tgcgcttgtt
     1681 gcaacagcgt cttcaaatcc gccccggcga tgaagatcga ttttttcgcc gatgcgatga
     1741 tcaatcctcg cagcgaggaa tcgttctgca caaagtcgag atgttcattt aattcgtcga
     1801 gagtcgcggc gtcgaaaatg ttcgcgcccg attcggggcg atcgaaggtg agcaggcaaa
     1861 tgtgatcgtc gccgatctca cggcgtatca ttggccctgt agcaactgca ggcatggcaa
     1921 ttaccttggc tctttttcgc gcaaattcca agcggaacag cactggaata gtggagtaat
     1981 ggagtgagga gacaaatgcg acagatggaa cggacaacat tattccatta ctccagcgct
     2041 ccagtcttcc actgctgctc cgttgctttg ggcgagcccg agtgttaaaa acgcgacgtg
     2101 gtcggcaaaa ttttagaacc ggaaataaag agcatgatcg acgcccgtaa cttcggtgcg
     2161 ttgcgcgaag tgtttcgaga ctgggcgccg gccgatgtcg cggaagtcat cctcgatatg
     2221 ccggaagacg aacaggtcat catctttcgg gtcctgccgc atgcgttggc cgcggacgtt
     2281 tttgaatatc tcgatttcga cgcgcagcag aaactgctgc gcgccatggc gcacgagcag
     2341 gtcgtcggaa ttctgaacga aatggcgccg gacgatcgca cggagctgct ccaggaattg
     2401 ccgagcgccg cggcgcgcca gttgatcaga tcgctaacgc cgcaggaacg ccaaatcgca
     2461 caggcgttgc tcggctatcc ggagggcagc gttggccggt taatgacgcc ggacttcatt
     2521 gcggtgcgcg aagattggac agtccaacaa gtgctcgatt atatccgcga acacggtcag
     2581 gacagcgaga cgctcaacgt gatctatgtg ctcaatgagc ggggcaaatt gatcgacgat
     2641 atccgcatgc gcgagttttt gctcaagccg ctcagctccg ctgtccgcga tttgatgcaa
     2701 aaaacgttcg tcgcgttgaa tgtgaccgat tcgcagcagg acgcactgaa cgtttttcgc
     2761 aaatacgatc gcaccgcgct gccggtgatt gactcgagcg gcgtcctttt gggaatcgtg
     2821 acgatcgacg acatgctcga cgtggccgag gcagaagcaa ccgaggacat ccaaaaattc
     2881 ggtggcgtga ccgcgctgga cgagccttac atgcggctgt ccctcacggg aatggtacgc
     2941 aagcgcgcac cctggctagt tattcttctg ctcggcgaaa tgctcactgc ctcggccatg
     3001 gcatattacg aggaagaact agccaaggcg ttagtgctgg cggtgttttt gcccctcatt
     3061 atcgccagcg gtggcaactc cggttcgcaa gcatctactc tgatgattcg agccatggca
     3121 ct
//
LOCUS       DASQUQ010000076        18052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_192422, whole genome shotgun sequence.
ACCESSION   DASQUQ010000076 DASQUQ010000000
VERSION     DASQUQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 18052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18052
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192422"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VE486_02130"
     CDS             complement(<1..306)
                     /locus_tag="VE486_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37916.1"
                     /translation="MLESTLRAAAPPDVALPVDETGSELKRGAFVNTIAMLASNFRGI
                     FTFLVARLLGPAALGTYLVAWATIELLSKIGLFGLDNTIVAFIARSEAADDRARSHAL
                     "
     gene            complement(826..1545)
                     /locus_tag="VE486_02135"
     CDS             complement(826..1545)
                     /locus_tag="VE486_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA37917.1"
                     /translation="MNAEICLSCRDIERYLGEEEERVHALRGVSLDLEPGSIHAVVGA
                     SGCGKSTLLYILGLLDPPDAGQVAIESEQVSHLSDDALAEKRSRFIGFIFQFHFLMED
                     FTAQENVMIPMRKLGQLSDGEMRERAAGLLAAVGLGDKLRRPSRHLSGGEQQRVAIAR
                     ALANDPRVILADEPTGNLDTANSERAFELLQGIVQQGGKALFLATHNPVIAEACDWIH
                     EMKDGRIIASHPRGTQISIFK"
     gene            complement(1542..2777)
                     /locus_tag="VE486_02140"
     CDS             complement(1542..2777)
                     /locus_tag="VE486_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA37918.1"
                     /translation="MTPPLYIALRFLTHRKRALLLSLSGVVFGVAIFICTQAQTQGFA
                     QHFIDSTIGSNGALVIKSRFRPRYEPLMVSSKSSNGKAPRRLYFEGITDANEIMRVSR
                     RFSNVVSCSPVLRGTVSARAGFENATVDLYGIEPALHAQTTDLLHQLVEGSFDDFRNN
                     TSAIIVGSRLAEILQAGIGDTVQLLSPNGEYWRFTVAAIARAGVGSIDATRIYSHARI
                     AQSLLQRPFGASMIIYKLRDPDRAPALARHFETLFQHGASSWQEREQSTLQLFLTLRM
                     SAAITVSLIILLAGFGIFNVLTMSVLAKIKEIAILRSMGYRRMDISAIFLWQGALIAV
                     AGSIVGCLLGALMTWGVSHIPIRIRGLLFSDYFLVTWDWRHYVFATLLAMLAVFIASY
                     VPARRAAQLAPVATIRGAR"
     gene            complement(2774..3925)
                     /locus_tag="VE486_02145"
     CDS             complement(2774..3925)
                     /locus_tag="VE486_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZA37919.1"
                     /translation="MPESKRVLWGGAALLALVFAGAYYFLMPVAEVATVRRGTAISAV
                     YGTARIEPAFVVHVRAQNSGFIQLAEPFSAGRGAIGKSVEKGQLLATIADETTARQLK
                     QARADLDAAVKRAELSLPSSDLLKAAEDNLQRLEKVVSAGSVPVVEYEKAKSEVNRLR
                     SAVETERIERDRNLNALGEAAKKLEAQMKSAQVQSPIDGLLTNVQTIDGELVSAGNEL
                     FTVSSRKNYVRGEVNEEDVGEVKPGMKAKLQLYAYRNRTFTARVSSVQPAADPTTQRY
                     TIALEMENPPDNLMAGMTGEMNIITGTHENVLLVPTRALIVDQALIVRRGIVRSRTVQ
                     VGFRTLDFAEAISGLSEGNRVVVANQDKLRPGQPVRQRMVSMPPLPKEP"
     gene            complement(4124..5599)
                     /locus_tag="VE486_02150"
     CDS             complement(4124..5599)
                     /locus_tag="VE486_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZA37920.1"
                     /translation="MKQRILFLGSTGRWPNAQGGKLAKWRKLFAASCREQQAGSLCSP
                     EHCVRRLGRLAAPKLLITILMACLAHTTGAVTLETVLQTTLEKNPTIQAAKSGVEQAA
                     GQRLVLRSMIWPEVKMAVPAGVQGGHRAGEDDTKVFAFARGFFTQTLFNAAVPPSLRR
                     GDVDFLIAQQQLNVAVVDQLHRARLAFYTALYNRSLQSIREEQRRTLEENFATQKNRY
                     EAGLTDRTALTSATVQTRELVSEIESAHRAYLEAQLILADAMSNDPEKTALPEPEGDL
                     KIAPVFLNVDSEAAVALERRADLKLARLLVRAANEDQRIIEAGYYPAVTGEITGDYIP
                     ITGIHREGSTSRTQDFISSEIREKAGYTWRVVDNGKVRGAALRARATREINELTCRKL
                     ETDVRRELAHIANSLKGIESRERSLATASAAAQESATAVQKNLASGLGSPLEYRVTQN
                     AFLETKSGLLNAVYQYNLAVAEWDRATGRYFQFSDDPARSR"
     gene            complement(5596..6987)
                     /locus_tag="VE486_02155"
     CDS             complement(5596..6987)
                     /locus_tag="VE486_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZA37921.1"
                     /translation="MRLEPGPVVLLASLVLAVPMRATPTYTIEQTVALAQAQNPEIAI
                     ARKTVQAARGGLIEARSGFLPWVASTGFYDKRQQQKETRLRDEDYNATLRVEQNLYTG
                     GAVPSQVAIARLNIEKQGYEFQEVADRVTMQVRIAFNELLLNRAKIQVREASVRVLEE
                     ELKSQRERLGAGIVGALNVQRAEVALANERPELIDAQTQLKNSYLRLAELLGSDFRPG
                     SERPPFEIAGELQYRSPHPDLNECLARAATNRPLIKSRQIDVQIQDHQYTLDRSELRP
                     RVRAFSGYEVYSERDPAVGQEFNNGYVVGINATWNVFDGFATKGRMQATRARREAAVQ
                     ALAAARRAVASEVRSAFFDLEQADRVLETETKNVQTADEALEMAKGNLAAGLGTQLDI
                     LQAASDVTRTRTTRLGAIYLHNVALARLARACASSAEALDFGSNIKKARTENPNAAQA
                     TDVARPPAKLSQR"
     gene            7210..8307
                     /locus_tag="VE486_02160"
     CDS             7210..8307
                     /locus_tag="VE486_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZA37922.1"
                     /translation="MRKIRIAIVGVGNCASSLVQGINFYGRSSGDGAGLMHRQVGSYR
                     PSDIEVVAAFDIDRRKVGLDVSKAIFSQPNCTKVFCEKIALTGAIVKMGCVFDGYPPH
                     MRDLDPLRTFLPLEKESSREQIIAELKNSSAELMVNYLPVGSEEATRFYAGCALEAGL
                     GFVNNIPVFIASDPTWAKRFADKNLPLIGDDIKAQMGATILHRMIVDLFRKRGVKLDR
                     TYQLNTGGNTDFLNMLNRTRLASKKTSKTEAVQSVIGERLADENIHIGPSDYVPWQLD
                     NKIAFIRTEGRLFGDVPMEMDVKLSVEDSPNSAGVAIDAIRCCKLALDRGIGGVLHSA
                     SAYFSKHPPVQMTDDEAYRCVEQFIRGERDR"
     gene            complement(8304..11453)
                     /locus_tag="VE486_02165"
     CDS             complement(8304..11453)
                     /locus_tag="VE486_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZA37923.1"
                     /translation="MTPIADETARNRFARELDRNFSVIASAGSGKTRAITNRIVEVAK
                     NRQRALEWLPQLVVVTYTNRAADEMQQRTRQRILEAGLPLETVEAFGRAFFGTIHAFC
                     MKLLATHGHHLGLPARLETITDDDDLWNEFVQQQTEIGRSLGTENRRLLLRLMQARQL
                     MDLARRQDHDFTAPAPLGLCPECDFSKVEAALESAQAKNIPKAKAQLRDWLRRWRETD
                     DFAPWPPCATTAREFTARWREAFRPLREWASACASSVAADVQRDYRQFRLARGAITYP
                     DQVALAMELLRLPNLAQRIRGKNYRVVLDEAQDTDPQQFLVLVEVARPVEARGEWLQD
                     RQSPPRSGHFSMVGDFQQSIYRDPADLAHYRQLHRALVETDAAKELKFSVTFRLDRAQ
                     LDFINDTFGNILNNTEGQVEFVEMNPRPEILAGQVIRFDLGEVVDLSLPETQRARIEA
                     ERLAEWLRDRGLPKLRARCWREVAILCPRKAWLRALRDALIEQQIPVEVQSESDRQAE
                     HPAYAWLTALLAIMVDPNSGYEIVGVLREVFGISDDELARFAQGDGSKFQIERKTAGR
                     GVVVDALNLLTRLRGKLAHQPLFSAAREIVRMTQLRERLRKLPREEFGDSLNELDKLL
                     SSTAAAESRQESLADFALVLRRNFDKIRETEPAENDAVQLITAHKAKGSEWDAVIVPF
                     LAREARLGGNIRYPMVLPGEQPFIAFDRSDRADLEEELKRIQRQEMERLLYVALTRAR
                     HTLVFALDVDFFRGKRGVHSDTQLRWLQADANEPNAKAISAVPEEPTSCEETATRHSE
                     REPEVVADTLSALRLETGWIDIARQRAASIIETFTPSQFAPEEETTETPSTEEWIEIE
                     PELRPPRIDNPATRYGVWWHDFAQQIRWRSEPAGWQSIFDHNVTASPDVARSKREWQL
                     LQKQIESSGELARHLRGASLVRKEMPFFWKINGSSWNGEKCLEGIVDLALFNQKANEV
                     FILDWKTNRIAADKIDILKDIYRPQMAAYWQAMSGLTNALVSAAIYSTATGEFLVYER
                     DELIREWERLRDLAQNGLA"
     gene            complement(11450..14518)
                     /locus_tag="VE486_02170"
     CDS             complement(11450..14518)
                     /locus_tag="VE486_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZA37924.1"
                     /translation="MPQKTSKGRFPRVSLLLTTSPEAAWENVVRPWFEKIATRGLREE
                     QPAVVVTASRSQAYFFRSRLLAEGKSLLGVKFLSPPQLRELLLRGPDPKLPLREHLRL
                     FLATAAEELASGNGNNEAAFVAKSIARDPDHFLRAVDQLRAAGWSFDEIDSPPLRAIA
                     AQFEKQVRECGFTFVYDADRDAAQSAPESSPLFSNLLLFGFDAAHWPLWPLLRTATLV
                     STSATAVLNDPRDEARDLDETWVGTWEENFGPAEILPPPSAASRLPLPDHEAKGRLDL
                     LTQSLPVDIHFLIGRDTTQQARAIVALTAKFLSDPQCERLGILFSRPSALARLVATFL
                     QSAKIPHNDGIAHLAPSAFDDDAWRAWLELQEAPRLKLLFRFLRATSAEIFEKISILQ
                     VEETLRRAYNDILIDDIELLGDYCAGSPDLRHGVAVARGLEKIQFLPETALFVEFLSQ
                     TRKIFWQLGWKEHWHEVERLSRNWSERLSNKVSKSSYLRWLRELLGAASLERDDFGAH
                     PYARVHLLPYADAQGQPWSHLILAGLNDETWPAFDEELAFVRDERVVELNRLNRTLNR
                     RASKRGRQGEGHWSVCEGKTLLLGPNEQRQLRRRQLLNLVESVSNAIGASANLYSEAT
                     PSRIANPSEFFSWLYFNACGRGVSQRTLQTLEQQTRGWLKDWSPVDAQKVDSISVGRT
                     RYAFDQRRRLRAAGEYEFALRNPSERDISLRVTQWEQALRWPAIVWMEIFLGVEPDDD
                     NGDTWAVATGQWVHRWLADSVPNSHERKFVDVNQADEIRERVRTHARGFREQVSKLCA
                     GRSRALPDWWRSGWSNALYIADCLAAKISDLRDWSEMAVEWALDEPSLIPVSKNETLR
                     VRGRIDLILARRKSEQAKIGFSDLWVVDYKTGRQRGFRLKELRRKDMPERKFRRQLIE
                     GRGVQLALYALAVHALGAEEVQLTLLSPAEEFEAQFHLADVLAQKDFWLELHWMQETG
                     VFGMLGPVHADFGYVRKYPLATLPIDPDLLEEKWGLTHRAFSVEDEEEAK"
     gene            complement(14546..16459)
                     /gene="selB"
                     /locus_tag="VE486_02175"
     CDS             complement(14546..16459)
                     /gene="selB"
                     /locus_tag="VE486_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZA37925.1"
                     /translation="MIETHFILATAGHVDHGKSALVKALTGIDPDRLPEEKARQITID
                     LGFAQLILEGPGEQRFQIGIVDVPGHEDFVRNMIAGVGSIELALFVVAADDGWMPQTE
                     EHLQILTYLGVQRAVIALTKSDLGDVETTEQEINNQLRNTAFAQSPIIPTSVRTGKGI
                     ENLKSALASEFATTQPQRDFGKPRLFIDRAFTLRGIGTVVTGTLTGGQLHRGQSVVVE
                     PQGFEARIRSIQSHRSELESAQPGMRTAINLPDIAIGDGLMQVKRGDVITAVGIGRAS
                     STLVARIEKSGRLNRENPAARPLKNGSSVYVHHGTSRFPAKIHLLDSDALEPGKQALA
                     KLKLGSPIFAFAGDRFVLRDASEQHTIAGGVVLDPDGDRGKSGNAAQHKLLAARTVAP
                     DDIDPCVRSEIALRGFVRTETVLRKSHFSSAEIADALQRLQRDNEIVIHGGIAAAPET
                     WKTLRSRASFLIDDAHKKNPALAGLDLNELRTALRDQPADIFEGLIADLCADNFVRKG
                     STIARIGHRPALPAELQPIEGRIREALAKKPFDPPPRREIETDLLSQRVLRFLIEMGE
                     VIEIASDVVLLRENFERMKSAVTDFITKNGPATVSELRQALESSRRTMVPFLERLDRE
                     GFTRRMGDKRSLR"
     gene            complement(16544..17935)
                     /gene="selA"
                     /locus_tag="VE486_02180"
     CDS             complement(16544..17935)
                     /gene="selA"
                     /locus_tag="VE486_02180"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZA37926.1"
                     /translation="MHDRIKTRSRREIPAVSNLLDALGHYDFPRPVIVDLIRRELATI
                     RAGAEIPEFESIVDSVRCSLANLRATRLQSVINGTGIVIHTNLGRAPLAPEAVRALQE
                     IGPAYSNLEYDLVACGRGRRGSYIENALALLCQAESATVINNCAAALTLIVHHFTKGK
                     ARAPGAPEVVISRGELVQIGGGFRIGEIIEAAGAKVREVGATNKTTLPDYAKAVGQNT
                     ALILKVHRSNFFMDGFVESPSTAEIAGIARKKRIPFLEDLGSGAIVATEQFGIAEHEP
                     TPAEALKNGADLVCFSGDKLFGGPQAGIIAGKKRFVAALKREPVFRALRCDKLVFAAL
                     QATIDLHLDQSTGTIPAIALLQVSEDELRNRAAAIVGRLEGLPLRIAIGCGTVKVGGG
                     TLPKSTMSSVTIDIVPKNCSLADFAARLHTSNPPVIGYIANARFKLDLRTIFPQQDDL
                     VVDAIRSVSRSAQ"
BASE COUNT         3745 a         5304 c         5018 g         3985 t
ORIGIN      
        1 tagggcgtgg ctgcgagcgc ggtcgtcagc ggcttcggat cgcgcgataa aagcgacgat
       61 cgtgttatcg agcccgaaca aaccgatctt gctcagcagc tcaatcgttg cccacgcaac
      121 gagatacgtg ccgagagcag ctggcccaag gagtcgcgcg acgagaaaag taaagatgcc
      181 gcgaaagttc gatgccagca tcgcgatcgt gttgacgaac gcgccgcgtt ttagctctga
      241 acccgtctcg tcaacgggta gcgctacatc gggcggcgcc gcggcgcgca gcgtgctctc
      301 aagcatgctc actgacttgg gctgctgcca ttctcgggtg aagtggtgcg acgccattga
      361 gttgatccaa tcggtctgtc ctgcatctcg cgaatccagt tacaggcttg cgcgatttct
      421 atggttatat gtcatgagaa atagcgcctt gactcgtcct cggccgaaat ttgttcgaag
      481 tagcaggagc ctaaactaac attcgcgctc atatcgcctc gacgctacaa tcgagtgctc
      541 gggccaccaa aattttttcg caaagtgttc tggtcacgca tatctaagcg ttcgggcaac
      601 gcacgtccgg ctcgtattcg ccggttttta ctgcgatggg cgcctggttc catcgctcct
      661 ccaggcacag gccaactccg gtcttaccgg ctcagatgca ccctcgattt tgcgccgctt
      721 gcgatccaca tctttcgcgc tcttcagatt cgctgcacgt tccccaacaa aattgccaca
      781 gcgatgtggt aacacaagct gggtgtgcaa ttgtcgattc taacgttact taaaaatcga
      841 aatctgcgtg ccacggggat ggctggcaat aatgcggcca tccttcattt cgtggatcca
      901 atcgcaggct tcagcgatga caggattgtg cgtggcaagg aacagagctt tcccgccttg
      961 ctggacgatg ccttgcagca gttcaaaggc acgctccgaa tttgccgtgt cgagattgcc
     1021 ggtgggctca tccgccagga tcactcgcgg atcgttggcc aatgcacgcg cgatagcgac
     1081 acgctgttgc tcgccccccg agagatgacg gctgggtctg cgcagtttgt cgcctaaccc
     1141 caccgctgcc agcaatcctg cagcgcgctc gcgcatctcg ccatcggaaa gctgtcccag
     1201 cttgcgcatc gggatcatca cgttctcctg cgcggtgaag tcttccatca ggaagtggaa
     1261 ttgaaaaatg aaaccgatga acctgcttcg tttctctgcc agcgcatcat cgctcaggtg
     1321 cgacacctgc tccgattcga tggcgacctg cccggcatcg ggcggatcga gcagaccgag
     1381 aatatagagc aatgtgctct tgccacagcc ggaagcgccg acgacggcgt gaatgctgcc
     1441 cggctcgaga tcaagcgaaa ccccgcggag cgcatgcaca cgctcctcct cttcaccgag
     1501 gtagcgttcg atatcgcggc aactgaggca gatttccgcg ttcatctggc gcctcgaatc
     1561 gtagctactg gcgccagctg ggcggcgcgc cgcgcgggaa catagctcgc gatgaaaacg
     1621 gcgagcatcg cgagcaatgt cgcaaaaacg tagtgccgcc aatcccaggt caccaggaag
     1681 taatcggaga aaagcaaacc gcgaatccga atgggaatat gcgacacgcc ccaggtcatt
     1741 agcgcgccga gcaaacagcc gacaatcgac ccggccactg cgattagcgc accctgccac
     1801 aggaaaatcg cgctgatgtc catgcgccgg taacccatcg agcgcaaaat tgcgatctcc
     1861 tttattttgg cgagcacaga catcgtcagt acattaaaaa tgccgaaacc ggccagcagg
     1921 atgataagag acacagtaat ggcagcggac attctcaagg tcagaaaaag ttgcagcgtg
     1981 ctctgttcgc gttcctgcca gctcgatgcg ccgtgctgaa acagcgtttc gaaatgcctg
     2041 gctagagccg gcgcgcggtc tggatcgcgt agcttgtaga tgatcatcga cgccccaaag
     2101 ggtctttgca ggaggctttg ggcgatcctt gcatgtgagt aaatccgggt ggcatcgatt
     2161 gatccgactc cggcgcgcgc gatcgccgct accgtgaaac gccagtactc gccattgggc
     2221 gaaagcaatt gcaccgtgtc gcctatcccc gcttgaagta tttcggccaa cctggaaccg
     2281 acgatgatcg cgctcgtatt atttcggaag tcatcgaagc tgccttcaac caactgatgg
     2341 agaagatccg tcgtttgcgc gtgcagcgcc ggttcgatcc cgtaaagatc gacagtcgcg
     2401 ttctcaaatc ctgcgcgtgc gctgactgta ccgcgcagaa ccggcgaaca ggaaacgaca
     2461 ttggaaaatc gccggctgac ccgcatgatc tcgttcgcat cggtgatccc ctcaaagtag
     2521 agccgtcgtg gcgctttacc gtttgagctt ttggaggaga ccatgagagg ctcgtagcgc
     2581 ggacggaagc gcgacttgat aacgagagcg ccgttgctgc cgatagttga gtcaataaaa
     2641 tgctgcgcga agccctgtgt ttgtgcctgg gtgcagatga agatggcgac gccgaaaacg
     2701 acgccactca ggctgaggag caaagcgcgt ttgcggtgcg tcaagaagcg cagggcgatg
     2761 taaaggggcg gcgtcatggt tccttcggca gcggcggcat gctcaccatt cgttgccgca
     2821 ccggttgccc aggccgcagc ttgtcctggt tggcaacaac cacgcgattg ccttcggaga
     2881 gaccgcttat cgcttctgca aaatcgagag tccgaaatcc aacctggacg gtgcgagaac
     2941 gcacaatgcc tcgcctgacg atcaatgcct gatcgacaat gagggcgcgg gtcggcacga
     3001 gcagaacgtt ttcatgtgtg ccggtgataa tgttcatttc gccagtcatt ccggccatca
     3061 gattgtcggg aggattttcc atctccaacg cgatggtata gcgctgcgta gtcgggtcgg
     3121 ccgcgggctg gacggatgaa acgcgcgcgg tgaaagtgcg gtttcgatag gcatacaatt
     3181 gcagtttcgc cttcatcccg ggtttcacct cgcctacgtc ttcctcgttc acttcgccgc
     3241 gaacataatt cttgcgcgag gagaccgtga aaagctcatt gcctgctgaa accagctcgc
     3301 catcgatcgt ctgaacattc gtcagcagtc cgtcgatggg cgactggacc tgtgcgctct
     3361 tcatctgtgc ctcaagcttt ttggcggctt ctcccaaagc attgagattt cgatcgcgct
     3421 cgatccgctc cgtctccacg gcgctgcgca gccgatttac ttcgctcttc gccttctcgt
     3481 attcgaccac cggcacgctc cctgccgaaa ccactttttc gagtcgctgg agattgtctt
     3541 ccgccgcttt gagcaaatcc gaagagggaa gagaaagctc agcgcgcttt acggcagcat
     3601 ccagatccgc gcgcgcttgc tttagctgcc gcgcggtcgt ctcgtcggcg atcgttgcca
     3661 gcaattgccc tttctccacg ctcttcccga tcgcgccacg cccggccgag aacggttccg
     3721 ctaactggat aaatccggaa ttttgagccc gaacatgcac cacaaacgcc ggctcgattc
     3781 gcgccgtccc ataaactgcg gagatggccg tgccgcgacg gaccgtagcc acttccgcca
     3841 ccggcatcag gaagtagtat gccccggcga aaacaagcgc gagcagcgct gccccgcccc
     3901 acaagaccct ttttgattcc ggcaatttca atttggaaaa ctagtgtggt gtccgtaaca
     3961 cctctttgca ataaactggt gcccgagcgt ttttaccctc atctttgccg tttggcagat
     4021 gctcgagaac gttattccca agacaccgca gcagaaacgc ccgttcgatc atcgaagggc
     4081 aattaagcgc cagacggtgg gcgcgaccgc ccggccgccg catctaccgc gatctcgcgg
     4141 ggtcgtcaga aaattgaaaa taacgtccgg ttgctcgatc ccattccgcg accgccagat
     4201 tgtactgata cacggcgttc agcagtccac ttttggtttc cagaaaagcg ttttgcgtga
     4261 ctcggtactc gagcggcgac ccgagaccgc tggccagatt tttctgcacc gcggttgcgc
     4321 tttcctgcgc ggccgctgac gcggtggcca gcgatctttc gcggctctcg attcccttaa
     4381 gactgttggc aatgtgcgcc agctcacgcc ggacatccgt ttccaacttt cggcaggtca
     4441 gttcgtttat ctcgcgggtt gcgcgcgccc tcagcgccgc cccgcgcact ttgccattgt
     4501 cgacaacgcg ccatgtatag ccagccttct cacgaatttc ggatgaaata aagtcctgtg
     4561 tcctgctcgt tgaaccttcg cgatgaatcc ctgtaatcgg gatataatcg cctgttattt
     4621 ctccggttac agcgggatag taaccggcct cgatgatgcg ctgatcttca ttggcggcac
     4681 gaaccagtaa ccgcgccagt tttaaatcag ctcgacgctc gagcgcaacc gcggcttccg
     4741 aatcaacatt aagaaacacc ggcgcaattt tcaggtcacc ctccggctcg ggcagcgccg
     4801 tcttttcagg atcgtttgac atcgcgtcgg ccaatatcag ttgcgcttcc aaatacgccc
     4861 gatgcgcgct ctcgatttct gagacaagct cgcgagtttg cactgttgcg cttgtcaacg
     4921 cggtgcgatc ggtcagcccc gcttcatacc gatttttttg agtagcgaaa ttctcctcca
     4981 gtgttcgacg ttgttcttcc cggatcgatt ggagcgatcg attgtagagc gccgtataaa
     5041 aggccaggcg ggcccgatga agctggtcca ctacggcgac attaagctgc tgttgggcga
     5101 tcaaaaagtc cacatcgcca cgccggaggg aaggcggcac ggcggcgtta aacaaagtct
     5161 gggtgaagaa accacgtgcg aaggcaaaca ctttggtgtc atcctcaccg gcccggtgac
     5221 cgccttggac gccggccggc actgccattt tcacttcggg ccatatcatg gaacggagaa
     5281 caagacgctg cccagccgct tgttcgacgc ctgatttcgc cgcttgaatg gtgggatttt
     5341 tttcgagggt cgtttgaagg acagtttcca gggtgacggc cccggtcgtg tgcgctaaac
     5401 aagccatcaa aatcgtgatt aagagttttg gcgctgcaag ccgcccgagg cgccgcacgc
     5461 agtgttccgg ggagcacagg ctgccagcct gttgttcgcg gcagcttgcc gcgaatagct
     5521 ttcgccactt cgcgagttta cctccctggg cattcggcca gcggcctgtg ctccccagaa
     5581 agagaatacg ttgtttcatc gctggcttag ttttgcaggc gggcgcgcta catccgtggc
     5641 ttgcgccgcg ttggggtttt cagtcctggc ttttttgatg tttgatccga aatcgagcgc
     5701 ttctgccgaa ctggcgcatg cgcgtgccag acgcgccaga gccacgttgt gcaaataaat
     5761 tgcgcccagg cgtgtcgtgc gcgttcgcgt tacatctgat gcagcttgca agatgtcgag
     5821 ctgcgttccc aagccggcgg cgagattgcc cttggccatt tctagagctt catctgcggt
     5881 ctgcacgttt tttgtttccg tttccaagac gcggtcagcc tgttcgaggt caaaaaatgc
     5941 gctgcgaact tctgaggcaa ccgcacgccg ggctgctgcg agcgcctgca cggccgcttc
     6001 acgccgggcc cgcgtagcct gcatccgccc tttagtggcg aacccatcga acacattcca
     6061 cgtcgcgttt atgcccacca cgtatccgtt attgaattcc tgaccgacag ccggatcacg
     6121 ttcgctgtag acttcatacc cggaaaaagc tcggacccgc gggcgcaatt cgctgcgatc
     6181 cagggtgtat tggtggtctt ggatttgcac gtcgatctgg cgcgacttga ttagcggccg
     6241 gttcgtcgca gctcgtgcga gacattcgtt cagatccgga tgaggggagc gatattgcaa
     6301 ttctcccgct atttcaaagg gcggccgctc ggaaccgggt cgaaaatcgc tccccaaaag
     6361 ctcggccagc cgcaggtagg aattttttaa ttgcgtctgc gcgtcaatca gctccggtcg
     6421 ctcattggcc agagccacct cggcccgctg aacattcaac gcgccaacga tgccggcccc
     6481 caacctttcc cgctggctct tcaattcctc ctccaggacc cgcacggaag cttcgcgcac
     6541 ctgaatcttc gcccgattca ggagcagttc gttaaacgcg atccgcacct gcatcgtcac
     6601 gcgatcggcc acttcctgaa actcgtagcc ctgcttctcg atattcagac gcgcgattgc
     6661 cacctggctg gggactgccc ctccagtgta aaggttttgc tctacgcgga gcgtcgcatt
     6721 gtagtcttcg tcccgaagcc gcgtctcctt ctgctgctgc cgcttgtcgt agaatccggt
     6781 cgaggcaacc cacggaagaa aaccggagcg ggcctcaatc aagccgcccc gggcggcctg
     6841 cactgtctta cgcgcgattg cgatttccgg gttctgcgcc tgcgcaagag cgaccgtttg
     6901 ttcgatggta tacgtaggcg tcgcgcgcat tggaacggcc aggaccaggc tggccagcaa
     6961 cacaacggga ccgggctcca aacgcatttc gtctatctcc ttgccagggc ctttcttgca
     7021 acaagaatgg ctgtggcttg tatccttata tcagtccttg caagaccggg cgatctcgga
     7081 caaattcgcc gcttgcaata gttggagccg ctacggacgg gcatgacgca ccttaattga
     7141 cgcggcctaa tttgtatctt gctccgattg atgcaatgcg aatagccttt tagccttatc
     7201 agtctagaaa tgagaaaaat aagaatcgcc atcgtcggag ttggaaactg cgccagctca
     7261 cttgttcaag gaataaattt ttatggccgc tcctccggtg acggcgccgg gctgatgcac
     7321 cggcaggtcg gctcataccg gccgagcgat atcgaagttg ttgccgcgtt cgacattgac
     7381 cggcgcaaag tcggtcttga tgtgagcaag gccattttct cgcagcctaa ttgcacaaaa
     7441 gtcttctgcg aaaagatcgc ccttaccggt gccatcgtga agatgggttg cgttttcgac
     7501 ggctatccgc ctcacatgcg cgatctcgac ccgctccgca ccttcctgcc gctggaaaag
     7561 gaatccagcc gcgaacagat catcgccgaa ttaaagaact cctccgcaga attgatggtt
     7621 aattatctcc ccgttggctc cgaagaagcg acccgctttt acgccggttg cgccctggag
     7681 gcggggcttg gtttcgtcaa taacatcccc gtcttcatcg ccagcgaccc gacgtgggca
     7741 aagcggtttg ccgacaaaaa tctgcccctc atcggcgacg acatcaaggc gcagatgggg
     7801 gccacgattt tacatcgaat gattgtcgat cttttccgca agcgcggcgt aaagctcgat
     7861 cgcacgtatc agctgaacac cggtggcaac accgatttcc tcaatatgct gaatcgcacc
     7921 cgccttgcct ccaagaaaac atccaagacc gaagccgtgc aatcggtgat cggcgagcgc
     7981 ctggcggacg aaaatattca cattggcccg agcgattacg tgccgtggca gctcgacaac
     8041 aaaattgcct ttatccgcac cgaaggccgt ctcttcggcg atgtgccgat ggagatggac
     8101 gtgaaacttt cagtcgaaga ttcgccgaac tcggccggcg tggctatcga cgcgatccgc
     8161 tgctgtaaac tggcgctcga tcgtgggatc ggcggcgtgt tgcattccgc ttccgcttat
     8221 ttctcgaagc atccgcccgt gcaaatgacc gacgacgaag cgtatcgctg cgtcgagcaa
     8281 ttcatccgcg gcgagcgcga tcgctaggct agcccgtttt gcgctagatc gcgcagccgc
     8341 tcccattcgc ggatgagttc atcacgctcg tatacaagga attccccggt cgcggtggaa
     8401 taaattgcag cgctgacgag cgcgttcgtg aggccggaca tcgcctgcca gtatgccgcc
     8461 atctgcggcc ggtaaatgtc cttgaggatg tcgatcttgt cggccgcgat ccggttcgtt
     8521 ttccaatcga gaataaaaac ttcgttcgct ttctggttga acaacgcaag gtcgacaatc
     8581 ccttcgagac atttttcgcc attccagctc gaaccgttga ttttccagaa gaaaggcatc
     8641 tctttccgaa ccaaactggc gccgcgtaaa tggcgggcca gttcaccaga cgattcgatc
     8701 tgtttttgca agagctgcca ctcccgtttc gaccgcgcga catctggcga cgcggtgacg
     8761 ttgtgatcga agatcgattg ccaccccgcc ggctccgaac gccacctgat ctgctgggcg
     8821 aaatcgtgcc accacactcc ataccgcgtc gccggattat cgatccgcgg gggtcgtaac
     8881 tctggctcga tctcaatcca ttcctcggtg ctcggcgttt cggtcgtttc ttcttcggga
     8941 gcgaactggc tgggggtgaa tgtctcgata atagaagccg ctctttgccg tgcgatgtca
     9001 atccaaccgg tctctaatcg aagcgcgctt agggtatcag cgacaacttc cggttcgcgc
     9061 tcgctgtggc gcgtggcggt ctcctcgcaa gatgtcggtt cctctggaac tgcggaaata
     9121 gctttcgcgt tcggctcgtt cgcgtcggct tggagccatc ttaattgtgt gtcgctgtgc
     9181 acgccgcgtt tgccgcgaaa aaaatcgacg tccagtgcga agacgagcgt gtgacgcgca
     9241 cgggtgagag caacgtaaag caaccgttcc atttcctggc gctgaatccg tttcaactcc
     9301 tcttcaaggt cagcgcgatc gctccgatcg aaggcaatga acggctgttc gccgggcaac
     9361 accatcggat aacgtatgtt cccgccgagt cgcgcttcgc gcgcgagaaa cggaacgatc
     9421 acggcgtccc actccgatcc ttttgccttg tgcgcggtga tcagttgaac tgcatcattt
     9481 tccgccggtt cggtttcgcg gatcttgtca aagttgcgtc gaagcacgag cgcaaaatca
     9541 gccaacgatt cttgccgcga ctccgccgcc gcggtgctgc tgagcaattt atcgagttcg
     9601 ttgagcgaat cgccaaattc ttcgcggggc aatttacgca gccgctcgcg caattgcgtc
     9661 attcgtacaa tctcgcgcgc ggcgctaaag agcggctgat gcgccagctt tccgcgaagc
     9721 cgagtgagca gattcaatgc gtcaacgact acgccgcgac cggcagtctt gcgttcgatc
     9781 tgaaatttgg atccgtcgcc ttgagcaaac cgtgccagtt cgtcgtccga gattccgaat
     9841 acctcgcgca gaacaccgac gatttcgtag cctgaattcg gatcgaccat gatggcgagc
     9901 agcgcggtta gccaggcata ggctggatgt tcggcttgtc gatcgctttc cgattggacc
     9961 tcgacgggaa tttgttgctc gatgagcgca tcgcgcaggg cgcgcaacca ggccttgcgc
    10021 ggacagagga tcgcaacttc gcgccagcat cttgcgcgca atttcggcag gcccctgtcg
    10081 cgcagccatt ccgccagtcg ttcggcttca atccgcgcgc gttgggtttc cggcaacgaa
    10141 aggtcgacaa cctcgccgag gtcgaagcgg atcacttgcc cggctaaaat ttcgggacgc
    10201 ggattcattt cgacgaattc aacctggccc tcggtgttgt tgagaatgtt tccaaatgtg
    10261 tcgttgataa aatcgagctg cgctcggtcg agtcgaaacg tgaccgaaaa cttcagctct
    10321 ttggccgcat cggtttcaac aagcgcgcga tgaagttgcc ggtaatgcgc gagatccgcc
    10381 gggtctcggt aaatcgattg ctggaaatcg ccgaccattg agaaatggcc ggatcgcgga
    10441 ggcgattgtc gatcctgcaa ccactcaccg cgcgcctcga cgggacgcgc gacttcgacg
    10501 agcacaagaa actgctgcgg gtcggtgtcc tgagcttcgt cgagcacgac acgataattc
    10561 tttccgcgaa ttcgttgcgc gaggtttggc agtcgcaaca actccattgc gagcgcgact
    10621 tgatcgggat aagtaatcgc gccgcgagca agacggaact ggcggtaatc gcgctgcacg
    10681 tctgccgcga cggacgacgc gcacgcgctt gcccattccc gtaaaggccg aaacgcttcc
    10741 cgccagcgcg cggtaaattc tctcgcagtt gtggcgcacg gtggccaagg cgcgaagtcg
    10801 tctgtttcgc gccagcggcg cagccagtcc cgtagctgcg cttttgcttt cggaatattt
    10861 ttcgcctgcg cgctttcaag ggcagcttcg actttgctga aatcgcattc tggacagagt
    10921 ccgagaggcg cgggcgcagt gaaatcgtga tcctgcctgc gcgccaggtc catcaactgc
    10981 cgcgcctgca tcaatcgcaa aagaagacgc cgattctctg tgcctaacga gcgtccgatc
    11041 tccgtctgtt gttggacaaa ctcattccac aaatcgtcat cgtcggtgat cgtttcgagt
    11101 cgcgcgggca gtccaagatg gtgtccatgc gttgcgagga gcttcatgca aaacgcgtgg
    11161 atcgttccaa aaaacgcgcg accgaacgct tccacggttt cgagcggaag gccggcttcg
    11221 agaatccgct gccgcgtgcg ttgctgcatt tcatcagcgg cgcggttcgt gtaagtgaca
    11281 accacaagtt gcggcagcca ttcaagcgcg cgttgtcgat ttttcgcaac ctcaacgatg
    11341 cggttggtga tcgcgcgcgt tttgccggac ccggcggaag cgatcactga gaaattgcga
    11401 tccaattcgc gagcaaatcg gttccgcgcg gtttcgtcgg caataggcgt cacttcgctt
    11461 cctcctcgtc ctcgaccgaa aaagcccgat gcgtcagccc ccacttctcc tcgagcagat
    11521 cggggtcaat cggcagcgtc gccagcggat acttccggac gtatccgaaa tcagcgtgga
    11581 ctgggccgag catcccgaag acgccggtct cctgcatcca gtgcagctcc agccaaaaat
    11641 ctttctgggc gagcacatca gccaaatgaa actgcgcctc gaactcttcg gcgggcgaaa
    11701 gcaatgttaa ctgtacctcc tcggcgccga gcgcgtgaac cgcgagcgcg tagagcgcga
    11761 gctgaacgcc gcgaccttcg attaattgcc tccggaactt tctttcgggc atgtccttgc
    11821 gccgcagctc tttcaaacga aaaccgcgtt gccgtccggt cttgtaatcg acaacccaga
    11881 gatcgctaaa accaattttc gcctgctcag attttcgccg cgcaagaatc aaatcgatcc
    11941 gaccgcgaac gcgcagtgtc tcgtttttgc ttacgggaat caaactgggt tcatcgaggg
    12001 cccattcgac cgccatttca gaccaatccc ggagatcgga tatcttcgct gcaaggcaat
    12061 ccgcaatgta aagcgcgttg ctccaaccgg agcgccacca atcaggcaga gctctgctgc
    12121 ggccggcgca aagtttgctg acttgttcgc gaaatcctcg cgcgtgtgtc cggacgcgct
    12181 cgcggatttc gtctgcctgg tttacatcga caaattttcg ttcgtggcta ttgggaacgc
    12241 tatcggcgag ccagcgatgg acccattgtc cagtggcaac agcccacgtg tcgccgttgt
    12301 catcgtcagg ttcaacgccg agaaaaatct ccatccaaac gatcgccggc cagcgcaacg
    12361 cctgttccca ttgggtaact cgcaggctga tgtcacgctc ggacggattt cggagggcga
    12421 attcatattc gccggcagct cggagtcgcc ggcgttgatc gaacgcgtat cgcgtgcggc
    12481 cgacgctaat cgaatcaacc ttctgggcgt cgaccggcga ccaatccttc aaccagccgc
    12541 gcgtttgctg ttcgagcgtt tgcaacgttc gttgtgaaac gccgcgtcca cacgcattga
    12601 agtaaagcca ggagaaaaat tcgctcgggt ttgcaattct gctcggcgtt gcttcggaat
    12661 aaagatttgc gctcgcgccg atcgcgttgc taacggactc aaccaggttc aggagctgtc
    12721 gtcgacgaag ctggcgttgc tcattgggac caagaaggag ggttttgcct tcgcacacgc
    12781 tccaatggcc ttcaccctgc cgcccgcgct tcgaagcgcg tcggttcaat gttctgttaa
    12841 ggcgattcag ctcaaccacc cgctcatctc gaacaaacgc aagctcttcg tcgaacgccg
    12901 gccaggtctc gtcgtttaac cccgcaagaa tcagatgcga ccatggctgt ccctgcgcgt
    12961 ctgcgtaggg gagcaggtgc acccgggcat atggatgcgc tccgaaatca tcacgctcca
    13021 atgatgccgc gcccaggagt tcgcgcagcc accgcaagta ggagcttttc gaaactttgt
    13081 ttgataaccg ctcactccag ttgcggctga gtcgctcgac ttcgtgccaa tgttctttcc
    13141 agccgagctg ccaaaaaatt ttgcgtgtct gcgaaagaaa ttcaacaaac agcgccgttt
    13201 ccggcaggaa ctggattttc tccaagcccc gcgcgaccgc gacgccatgc cgtagatcag
    13261 ggctgcccgc acagtaatcg cccagcaact cgatatcatc gatgagaatg tcgttgtaag
    13321 cgcgcctcaa agtttcttcg acctgcaaga tcgatatctt ctcaaaaatc tcggcgctcg
    13381 ttgcgcgcag aaaccggaac agcagtttta gccgcggcgc ttcctgcaat tccagccagg
    13441 cgcgccaggc atcgtcgtcg aacgcgctcg gcgcgagatg cgcgatgccg tcattgtgtg
    13501 ggattttggc cgattgcaga aacgtcgcca ccaatcgcgc aagcgcgctc ggccttgaaa
    13561 ataaaatacc gaggcgttcg cactgcgggt cgcttaggaa ttttgcagtg agcgctacga
    13621 tcgcccgcgc ttgctgagtt gtgtcgcgac caatgaggaa atgaatgtcg actggcagag
    13681 attgagtaag taaatcgagc cggcccttgg cttcatggtc agggagagga agccgcgaag
    13741 cggcggatgg gggtggaaga atttccgccg ggccgaagtt ttcctcccaa gtcccaaccc
    13801 aggtttcatc gagatcgcgc gcttcatcgc gcggatcgtt caatacagct gtggcactgg
    13861 ttgaaacgag cgtcgccgtt cgaagcagcg gccagagcgg ccaatgcgcc gcgtcgaatc
    13921 cgaacagcaa caagttacta aaaagaggtg acgattccgg agcggattgg gccgcgtcgc
    13981 gatcggcgtc gtaaacaaaa gtgaagccac actcgcgcac ttgtttctcg aattgcgcag
    14041 cgattgcacg caacggcggc gagtcaattt cgtcaaaact ccagcccgcg gcacggagtt
    14101 gatcgactgc gcgcaaaaaa tggtccggat cgcgcgcgat cgactttgcg acaaacgcgg
    14161 cttcgttatt gccgttcccg ctcgcgagtt cttcggccgc ggtcgcaagg aaaagtcgaa
    14221 gatgttcccg caggggcagc ttggggtcgg ggccgcgaag caaaagttcg cgcagctgcg
    14281 gaggggaaag aaatttcacc ccgagcagcg atttgccctc agcaagcagc cgactgcgaa
    14341 agaaataggc ctggctgcgt gacgcagtta cgaccacggc tggttgttcc tctcgcaagc
    14401 cacgagtagc gattttctcg aaccagggcc ggacgacatt ctcccacgcc gcttctggcg
    14461 aagtggtgag aaggagagaa acgcgcggaa aacgcccttt gctcgttttc tggggcatac
    14521 ctcccatttg tcccatacgt cgggtctatc gcaagctgcg cttatcgccc atacgtcgcg
    14581 tgaacccttc gcgatcgagt ctttcgagaa atggaaccat agttcgccgc gagctctcca
    14641 gcgcctgtcg cagttcgctc accgtggccg gtccgttttt ggtgatgaaa tcagtgactg
    14701 cacttttcat gcgttcgaaa ttctcacgca gcagcactac gtccgaagca atttcgatca
    14761 cttctcccat ttcgatcagg aatcggagca ctcgctgcga tagcaaatct gtttcgattt
    14821 ctctgcgcgg tggcgggtcg aacggttttt ttgcgagtgc ttcgcgaatt ctgccctcga
    14881 tcggctgaag ttcggccggc aatgccggtc gatggccaat tcgcgcgatg gttgatcctt
    14941 tgcggacaaa attgtccgcg cacagatccg caatcagccc ttcgaagata tctgccggtt
    15001 gatcgcgcag tgccgtccga agctcattta gatcgagacc tgcgagcgca ggattttttt
    15061 tatgcgcgtc atcgatcaag aaactggcgc gactacgcag cgtcttccaa gtttcaggcg
    15121 cggctgcgat ccctccatga atgacaatct cattatcgcg ttgcaaacgc tgcaatgcgt
    15181 cagcgatctc ggcggaactg aagtgcgact tgcgaagaac ggtttctgtt cgcacgaatc
    15241 cgcgcagcgc aatttcggag cgaacacaag gatcaatatc gtctggtgcg acggtgcgcg
    15301 cggccaataa tttgtgttgc gccgcgttgc ccgatttccc ccggtcacca tctggatcaa
    15361 gaacaactcc gcctgcgatg gtgtgttgtt ccgaggcatc gcgaagaacg aatcgatcac
    15421 cggcgaatgc aaagatcggc gatcccagtt tcagttttgc aagcgcttgt ttgccaggtt
    15481 cgagcgcgtc actatcaagc agatggattt tcgctgggaa tcgcgacgtg ccgtgatgaa
    15541 cgtagacgga gcttccattc ttaagcgggc gagcggccgg attttcgcgg ttaagccggc
    15601 ccgatttttc aatgcgagcg acaagggtgg agcttgccct gccaataccg acagcggtaa
    15661 tcacgtcgcc gcgtttgacc tgcattaggc cgtcgccgat ggcgatatcc gggagattga
    15721 tcgctgtgcg catccccggc tgcgcggact ctaattcgga acgatgactt tgaatcgatc
    15781 ggatgcgcgc ttcaaaacct tgcggttcga caacgacgct ctggccacga tgcagttgcc
    15841 cgccagttaa tgtgccggtt acgaccgtgc cgattccacg aagtgtaaac gcacgatcga
    15901 taaacaaacg cggtttcccg aaatcgcgtt gcggttgcgt ggttgcgaat tcggaggcga
    15961 gcgcactctt caaattctcg attcccttac cggtccggac cgacgttgga ataattggag
    16021 attgtgcgaa ggcggtattt cgcaattgat tgttgatctc ttgttctgtc gtttcaacat
    16081 cgcccagatc gctcttcgtt agcgcgatca cggcccgttg cacgccgaga taagtcagga
    16141 tctgtagatg ttcttctgtt tgcggcatcc agccgtcgtc ggcggcgaca acaaaaagcg
    16201 cgagctcgat tgaaccgacg ccagcaatca tgttccgaac gaaatcttcg tggccaggta
    16261 catcgacgat gccgatctgg aatcgctgct cccctggtcc ttcgagaatc aattgtgcga
    16321 agccgagatc aatcgtgatt tgtctcgctt tttcttcagg caaacgatca ggatcaatcc
    16381 cggtgagcgc tttgaccagg gcactcttgc cgtgatcgac gtgaccggcg gtcgcgagaa
    16441 tgaaatgtgt ttcgatcata ccactttttg aaaccacgaa ggacacaaac agcacgaagc
    16501 ccgattggat tcgtcttcgt gtccttcgtg atcttcgtgg tggttattgc gcacttctgg
    16561 agacactgcg aatggcatcc actacgaggt cgtcctgttg cggaaaaatg gtccgcaaat
    16621 cgagtttgaa tcgggcgttt gcgatatagc cgatgaccgg tggattcgag gtgtgaagac
    16681 gcgcggcgaa atcggccagc gaacaattct tcggaacaat gtcgattgta accgacgaca
    16741 tggttgattt tggcaaggtt ccgccaccga ccttgaccgt accgcaacca atcgcgattc
    16801 gcagcggcaa cccctcgagt cggccaacaa tcgctgcagc tcgatttcgc aattcatctt
    16861 cagagacttg cagaagggcg atcgcaggga tggtgccggt cgattgatcg agatgaagat
    16921 cgatcgtggc ttgcaacgcg gcaaaaacca atttgtcgca gcgcaaggca cgaaataccg
    16981 gttcgcgttt caacgcggcg acgaaccgct tctttcccgc aataattccc gcttgcgggc
    17041 cgccaaacaa tttatcgccg ctgaagcaaa caaggtcggc cccattcttc aacgcttcag
    17101 caggtgtcgg ctcgtgttcg gcaattccaa attgttccgt cgcaacaatc gctccgcttc
    17161 ctaaatcttc gagaaatgga atgcgtttct tgcgcgcgat gccggcgatc tccgcggtgg
    17221 acggcgattc aacaaaaccg tccataaaaa aattactgcg atgaactttt agaatgagcg
    17281 ccgtgttttg cccaactgcc tttgcgtaat cagggagcgt cgttttattg gtcgcgccca
    17341 cctcgcgcac cttcgctccg gccgcctcga taatttcgcc aatgcgaaag ccgcctccga
    17401 tctgaacaag ttcgccgcga gagataacca cctccggcgc gcccggcgct cgcgccttac
    17461 ctttggtgaa atggtgaacg atcagcgtca gcgcggccgc gcaattgttg atcacggtgg
    17521 ccgattccgc ctggcatagc agcgcgagcg cattctcgat gtatgaaccg cgccggccgc
    17581 gcccgcaggc caccagatcg tattcaagat tggaataggc cgggccgatc tcctgcaggg
    17641 cgcgcaccgc ttccggagcc agcggggccc gtccaaggtt cgtatgaatg acgatcccgg
    17701 ttccgttgat gaccgactga agccgggtgg cgcgaagatt cgcaagcgaa cagcggacgg
    17761 aatcgacaat cgattcaaat tccgggatct ccgctcccgc gcgaattgtc gcaagctcgc
    17821 gccggatcaa atcgacaatc actgggcgcg gaaaatcgta atgaccgagc gcatcaagca
    17881 agttgctaac cgctggaatc tcccggcggg agcgagtttt tatccggtcg tgcatatcaa
    17941 tggcccgcgc cgccgtcgcg ccacgatttt tccgatcaca gctgcgctcg gcgtgcgtgc
    18001 ttttcgcagg aagttgagca ctttctccag gttcgcttca gcgacacaca ga
//
LOCUS       DASQUQ010000077         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_923471, whole genome shotgun sequence.
ACCESSION   DASQUQ010000077 DASQUQ010000000
VERSION     DASQUQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923471"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..2675
                     /locus_tag="VE486_02185"
     CDS             114..2675
                     /locus_tag="VE486_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZA37927.1"
                     /translation="MKSEITAAEQTLAVKENLRGGSLDPRAQPIGNSQSAIRNPKEPR
                     PATEDDESAAKYLFQLSRDVILSLDRTGNILCINQRGVQLSGYSETELRGANILERLL
                     LPEDHPAISQILHDLAQGKAREYEVRWRTKAGIVIHFDGVSVPRLSESGEFLSTLCTL
                     RDVTERKCAEEKLRKSEEKYRDLIEISPDAIYVVDANGICLLGNRAGAELAGIPQDEL
                     VGTPVTETYLPEERHLFRERLKKLEGKSALRFERKFVRKNGEIVPVEVSVSAIRGGYF
                     QAILRDISERKQGEALLTGEKRLLEMIATGVVLKEILNALCLIIEEQQSGTLASVLLL
                     NPDGVHLDSVAGPSLPKEWMQQMENVPIGPCAGSCGTAAYRAAPVIVSDIATDPLWDV
                     PEHRASALKHGLRASWSNPVLSSKGKVLGTFCMYYRETRTPNSQGLELLELATHLVQV
                     AIERDRADEALRASEQVARGQVEALTYSLDVLATAPAPDKFLGQMLSTIGRLLNAQSV
                     VLWLLDEPGGPLVLRAWAEGGNFAKTDPEHPFIKHPLSWKEDPGLQEMFFAGAPVACE
                     DVERDPNVSSALREYLLSGGTRKFLRIPTLVGGQVKGFIGIRHGARPPYRPEEIELAQ
                     ALAHQAMLAIQLNEFAEQSRQAAVLEERNRMARDIHDTLAQGFTGVIVQLEAAEDAIA
                     CCRGKEANAHLQRAGELARRSLNEARRSVHALRPEALEEANFWNALKGLIRNTTAGTA
                     LHTTFELRGKLRDLPLAWQENLLHIGQEALTNALKYAHSRNFETRLICNTKELRLELR
                     DDGDGFKVKDRHDGFGLAGMRERVEQIGGKLKITSARGKGTKVVVTLPYNREPML"
     gene            2672..3394
                     /locus_tag="VE486_02190"
     CDS             2672..3394
                     /locus_tag="VE486_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZA37928.1"
                     /translation="MKSKKKTVGTAKTSTKSPTSRATKAPKVKDVPRKKPRIAVLIAD
                     DHSVVREGLVSLIGRKPDMTVVGEASNGREAVDLWKQHHPDVMLLDLRMPELDGVSAI
                     KEIRADNENARIIVLTTFDGDEDIYRAIQAGAKGYLLKDVPREALMDSIRRVHAGETC
                     VPVHLAAKLAERVSGETLSAREIEVLKLMAEGKSNKEIGSALFISEGTVKSHVKAIFA
                     KMNVISRTEAVASATRRGLIQL"
     gene            3414..3833
                     /locus_tag="VE486_02195"
     CDS             3414..3833
                     /locus_tag="VE486_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37929.1"
                     /translation="MIYLKAFIAGFASTLVFHQGLLWLLYAGGVSARAPWNMTPVLPL
                     NVPGVISLAFWGGVWGIVLWALIRALRGGAYWAAALVIGALGPSLVAWFVVMPIKGMG
                     FAGGWDPKIIVGGLLLNGGWGLGVAVLMRLLNRVGPL"
     gene            4510..4716
                     /locus_tag="VE486_02200"
     CDS             4510..4716
                     /locus_tag="VE486_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37930.1"
                     /translation="MSTSANNSGEIRPYVELQQQIHDDLRVQHPEWVEPNGDCPTCES
                     YESRLAELLGLSSPSEFALGDPAV"
     gene            4746..5507
                     /locus_tag="VE486_02205"
     CDS             4746..5507
                     /locus_tag="VE486_02205"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZA37931.1"
                     /translation="MNTNTKKLNEKVAVVTGASKGIGAEIAKHFAAEGAAVVINYASS
                     KTDADKVVDEITKRGGKAIAVKANVAKKAEVEQLFAAAKKAFGKLDILVNNAGVYQFG
                     PLESATEGEFHRHFDTNVLGLILATQEALKHFGTEGGSVINVSSVASSATPPASVIYS
                     ASKAAVDAITGVLAKELGPKKIRVNAINPGPIETEGFHAQGFSGSDFERQAVAQTPLG
                     RLGQPRDVAPAAVFLASPDSAWITGETIRVTGGLH"
     gene            5547..>5732
                     /locus_tag="VE486_02210"
     CDS             5547..>5732
                     /locus_tag="VE486_02210"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZA37932.1"
                     /translation="MRAAQISKPGGDWELVERDIPEPGPGQVRVKVEACGICHSDMLV
                     KEGLWPGLQYPRVPGHEI"
BASE COUNT         1334 a         1522 c         1703 g         1173 t
ORIGIN      
        1 atgcttgcgt tttggagtgc ggagacatgt cgccgctttt aaaagcgcga acgtgtctcc
       61 atgcactcca acatccgctt gggagacata tttgcaccgg caattaactt attatgaaat
      121 cagaaatcac tgccgcggaa cagaccctgg ccgtcaagga gaatctacgc ggtggtagtc
      181 tcgatccgcg agcacagcca attggaaatt cacaatcggc aatccgcaat ccgaaagaac
      241 ctcgtcccgc gactgaggac gatgaaagcg cagcaaagta cctgtttcag ttatcccggg
      301 atgtgatcct ttccctggat cgcaccggga acatcctctg catcaaccag cgcggcgtcc
      361 aactcagcgg ctattccgaa acggaattgc gcggagcgaa cattttagag cggctgcttc
      421 ttcccgaaga tcatccagcg atcagtcaaa ttctgcatga ccttgcgcag ggcaaagcgc
      481 gtgaatatga ggttcgctgg agaacaaaag ctggcatcgt cattcatttt gatggcgtgt
      541 cggttccacg cctctcggaa agcggcgaat ttttgtccac cctttgcacg ctgcgggatg
      601 tgaccgagcg caaatgcgcg gaagaaaaac tgcgcaagag cgaagagaaa tatcgcgacc
      661 tgatcgagat ttcacccgac gctatttatg tcgttgacgc caatggcatc tgccttctgg
      721 ggaaccgcgc gggcgccgaa ctcgctggga tccctcagga tgagctggtt ggcacgcctg
      781 tcaccgagac ctatctgccg gaagaacgcc acctgtttcg agagcgactg aaaaaattgg
      841 agggcaaaag cgctctccgc ttcgagagaa aattcgtgcg caaaaacggg gagatcgttc
      901 ccgtggaagt ctcggtctcc gcaatacgcg gcggatattt ccaagcaatc ctgcgcgata
      961 tcagcgagcg caagcaaggc gaagcgttgc tgaccggaga gaaacggctg ctcgaaatga
     1021 tcgcgaccgg cgtggtcctt aaagagatcc taaatgcatt gtgtctgatc atcgaggagc
     1081 agcagagcgg tacgctcgct tcggtcttgt tgctgaatcc tgatggtgtt catctggatt
     1141 ccgtcgcagg accgagtctc cccaaagagt ggatgcagca gatggaaaac gtgccgattg
     1201 gaccgtgtgc cggctcttgc ggcaccgccg catatcgggc agcaccggtc atagtctccg
     1261 acatcgcgac ggacccgctg tgggacgtgc ccgagcatcg cgcttcagcg ctgaagcatg
     1321 ggttgcgagc ctcctggtcg aatccggtcc tctcgtcgaa aggaaaagtc ctgggaacct
     1381 tctgcatgta ctatcgcgaa acgcgaaccc caaactcgca aggcctcgag ttgctcgagt
     1441 tagcgacgca ccttgttcag gttgcaatcg aacgtgatcg cgctgacgaa gctctgcgcg
     1501 cttccgaaca agtcgcgcgc gggcaagtcg aagcattgac ttacagcctg gatgttctcg
     1561 cgactgcacc tgcgccggac aagtttcttg gacaaatgct aagcacgatc ggccggcttt
     1621 tgaacgcgca aagcgtcgtc ttgtggttgc tggatgagcc gggtgggccg ctcgtcttac
     1681 gcgcatgggc ggagggcgga aacttcgcga agaccgaccc ggagcatcca tttattaagc
     1741 atcctttatc gtggaaggag gaccctgggc ttcaggagat gttctttgcc ggcgcgcctg
     1801 ttgcctgcga agacgtagaa cgcgatccga atgtttccag cgctcttcgc gaatatttgc
     1861 tgtctggggg aaccaggaaa ttcctaagga ttccaacgct ggtgggcggc caggttaagg
     1921 gattcatcgg cattcgtcac ggagctcggc caccttatcg acccgaggaa atcgaactag
     1981 cgcaagcgct tgcccaccag gcgatgcttg caattcaact caatgaattc gccgagcaaa
     2041 gccggcaggc ggctgtgtta gaagaacgaa acaggatggc tcgcgatatt cacgatacgc
     2101 tcgcgcaagg gttcactggc gtgattgtgc aattggaagc ggccgaggat gcaattgcgt
     2161 gctgccgggg caaggaagcg aacgcgcact tgcaacgggc cggcgaatta gcgcggcgca
     2221 gtttgaacga agcgcgcaga tcagtccatg cgctgcggcc ggaggcattg gaagaagcta
     2281 acttttggaa cgctctgaaa ggattgatca gaaacacaac tgccggcacc gcgcttcaca
     2341 ctacttttga actacgaggc aaactgcgcg atctcccgct ggcttggcag gaaaatcttt
     2401 tgcacatagg gcaggaagct ttgaccaacg cgctcaagta cgcgcactcg cgcaattttg
     2461 aaacgcgatt gatctgcaac acaaaagaac ttcgcctgga acttcgcgac gatggcgacg
     2521 gatttaaagt caaagatcga catgatggtt tcggtctggc cggaatgcgc gagcgcgtcg
     2581 aacaaatcgg cggtaaactg aagatcacca gcgcgcgcgg caaagggacg aaggtggtgg
     2641 ttacgttgcc gtataaccgc gaaccgatgc tgtgaaatcg aaaaagaaaa cggtcggaac
     2701 ggctaagacg tccacaaaga gcccgacatc gcgagcaacg aaagcgccga aagttaaaga
     2761 cgtaccgaga aaaaaaccac gcattgcagt tctcatcgct gatgatcaca gcgtcgtgcg
     2821 ggaaggatta gtatcactga ttgggcgcaa accggatatg acagtcgtcg gtgaagcaag
     2881 caacggacgc gaagcggtcg atctttggaa acagcatcat cccgatgtga tgctactcga
     2941 tctgcgcatg cctgagctcg atggcgtcag tgcgattaaa gaaatccgcg ctgacaatga
     3001 gaacgcgcgc atcatcgtgc tcacgacgtt cgacggcgat gaagatatct accgcgccat
     3061 tcaagccggc gcgaaaggct atcttctcaa ggacgtgcct cgcgaggcat tgatggatag
     3121 catccgccgc gtgcacgccg gcgaaacctg tgtcccggtg catcttgcag ccaagttggc
     3181 agagcgcgtc agcggcgaaa cactgagcgc gcgagagatt gaagtgctca aactgatggc
     3241 tgagggaaaa agcaacaaag agatcggctc ggccctcttt attagtgagg gcactgtcaa
     3301 gtcgcacgtc aaagccatct tcgccaagat gaacgtcatc agtcgcaccg aagccgtggc
     3361 cagcgccact cggcgcggtc tgattcagct gtaggtcttc tcccttcgcg gccatgatct
     3421 atttgaaagc attcatcgcc gggtttgctt ccacgctcgt gtttcaccag gggttgctgt
     3481 ggctgctgta cgcaggcgga gtttcggcgc gagcgccttg gaacatgacg ccggtcctgc
     3541 cgctcaatgt acctggagtg atttccctgg cattctgggg tggcgtctgg ggcattgtgt
     3601 tgtgggcttt gatccgcgct ttgcgtggcg gggcatattg ggccgcggcg ctcgtaatcg
     3661 gtgcgctagg cccgagtctg gtcgcgtggt tcgtagtaat gccgataaag ggcatgggct
     3721 tcgcgggggg atgggatccg aaaatcatcg tcggcggatt gctcctgaac ggcggttggg
     3781 gactcggcgt tgcggtgctg atgcggctgc tgaaccgagt cggtccccta tgaaggaaaa
     3841 attgcactcg ttactggtgg caatagcggc attggccttg ccgatacacg tcatcaatcg
     3901 gagtgaggct gtggccagcg ctacgcggcg cgggctgatt cagttgtagt gcttgtcccc
     3961 tcgcgctacc atcattccat ctgcaaaggg acttctcttt agtcctcacc tttaaagatg
     4021 aattcgcctg agaattcagt ggcgtttggt gcgcctggta tcgagccacg ctggagttcg
     4081 agtgcgaagg aaggcgtcgg tacagcttac cacaccagct gccggatggt tcagtgctta
     4141 catttacatt gttttggaag cgcgatcagc gctgggaagg caggaactgg caggtgagcg
     4201 ttggaagata gccttccgag gctgtcatgg cagacgggct tcccagcctg tcgaatatct
     4261 gaacgtgcgt cggcgacaat cctcggcagg ccagcggccc gcctgccatg acaggcaaga
     4321 tgcctgtctt ccgatcactt ccggactcga cacttcagcg cgcgaaaatg cagctgagcg
     4381 tccgtcatga acaggccttc gttctctccc aaacgaggga gtgcagatca cctcgcgagt
     4441 ggtactggaa aatccttcgc acgcgcaatg gccggcgtct catcgtcgcc gatatatttg
     4501 aggatgaaaa tgagcacgtc tgcaaacaac tctggcgaaa tccgccctta cgttgaactc
     4561 cagcaacaga tacacgatga cttgcgtgtg caacatcctg aatgggtcga gcccaatggt
     4621 gattgcccta cgtgtgagtc gtacgagtcg cggctcgcgg aactactcgg tctttcttcg
     4681 ccgagcgaat tcgccctggg cgacccggct gtctaatcaa ctctgccaaa gaacgacatg
     4741 aaaatatgaa tacgaataca aagaaactta atgaaaaagt tgcggtggtg accggagcct
     4801 caaaaggcat cggcgccgaa atcgccaagc atttcgccgc agaaggcgcg gctgtcgtga
     4861 ttaactacgc gtcgtcaaag acagacgcgg acaaagtcgt ggatgaaatc acgaaacgcg
     4921 gcgggaaagc tattgcggta aaggcaaacg tggccaagaa ggcggaagtc gagcaactct
     4981 ttgccgcggc gaagaaggcg ttcggcaagc tcgacatcct ggttaacaac gccggcgtct
     5041 atcagttcgg acccctcgaa tcggcaaccg agggagagtt tcaccgccac ttcgatacca
     5101 atgttctcgg tctgattttg gctacccagg aagcgctaaa gcacttcggt acagagggcg
     5161 gcagcgtaat caatgtcagt tccgtggcca gcagcgcgac accgccggcc tcggttatct
     5221 atagtgcaag caaggctgcg gtggatgcaa tcacaggggt cctggcgaag gagcttggac
     5281 caaaaaagat ccgagtgaat gcgatcaatc ccggcccgat tgagaccgaa ggttttcatg
     5341 cgcagggatt ctccggaagc gattttgaga gacaggcagt cgcgcagacg ccgctaggac
     5401 ggctcgggca gccacgcgat gtggccccgg cggccgtctt cctcgcctcg cccgacagcg
     5461 cctggatcac cggcgaaacg attcgtgtca cgggcggact tcattgatta cggggctgga
     5521 taacgaaggc aattatgagc gctaaaatga gggcggctca aattagcaag cccggtggcg
     5581 attgggaatt ggtggaacgc gacatcccgg agccaggccc tgggcaagtt cgagtgaaag
     5641 tagaggcgtg cggcatttgc catagcgata tgcttgtcaa agagggtttg tggcccgggc
     5701 ttcagtatcc tcgtgttcca ggtcatgaaa tc
//
LOCUS       DASQUQ010000078         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_472948, whole genome shotgun sequence.
ACCESSION   DASQUQ010000078 DASQUQ010000000
VERSION     DASQUQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472948"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VE486_02215"
     CDS             <1..235
                     /locus_tag="VE486_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3 C-terminal
                     domain-containing protein"
                     /protein_id="HZA37933.1"
                     /translation="LQFRGREMAHQELGMQLMEKVRDDLSSMSQVEMEPKITGRNVTM
                     TLSPLPANRRKRRFTSPSKAADEGEPAVLPDGT"
     gene            complement(362..997)
                     /locus_tag="VE486_02220"
     CDS             complement(362..997)
                     /locus_tag="VE486_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA37934.1"
                     /translation="MKKVCAFTLVTALALCASQVCQAQASKDAKEANKLAREGAEASR
                     DQEWDKAVDLLREATALDRKYADELAAVYQQRGYAAATNQQFNDAISDYSEAIKIKPE
                     DARIYEQRAAVEMKTNDYDKALADYSEAIKLKPNETRYYLYRGYIYETKGDIKSSMAD
                     TEQALKLDPGNADAKSRKERLEKRQSINTPLPLSPIPAASPKASPTPAKKP"
     gene            complement(1071..1925)
                     /locus_tag="VE486_02225"
     CDS             complement(1071..1925)
                     /locus_tag="VE486_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HZA37935.1"
                     /translation="MLTVLFLGDVVGEPGRKGVIARLPELKEKHALDFVIVNGENAAG
                     GRGITGKITIELLRAGVSVVTTGDHIWDQKDIVPFLALEPRLLRPLNYPDGAPGSGSI
                     VLETAKGKIGVINVQARTFMQPILENPFHAVEAAVAKMRRETVNIIVDAHGETTSEKI
                     ALGRFLDGKVSAVIGTHTHVQTADEQIFPGGTAFLCDAGMCGPVNSILGRAIEPIMNR
                     FVSNLPASFPVATGEVRLRGALIEIDETTGRAVRITRVDKPGPGEAAVPEPEPKIDIE
                     QVPSPEQA"
     gene            1962..>2935
                     /locus_tag="VE486_02230"
     CDS             1962..>2935
                     /locus_tag="VE486_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37936.1"
                     /translation="MFHFTKVAQAARRCLANDEARSIAARHSSFVIPSSFAIRASSFL
                     LSIFPFALQAADNISILGSKPKWQVLEHYQETITHDEFSRLIQDVYCTHGFAPDLIEI
                     NEKTARVLMNREKQKFFTLRFAEDAASRDPVPRLWRPAESLPPAKPEKPLSGLRIALD
                     PGHLGGKWAKMEERWFQVGSSAPVQEGDLTLRVARLLAPRLRKLGARVFFIRNSNEPV
                     TAKRPDDFRELARKILIRNGVPQPLPDALDPNDPEKEQTIRWQSEILFYRYSEIRRRA
                     ARMNFKLHPDLVLCLHFNAEGWGDPNSPMLLDANHLHLLVNGSYLQDEL"
BASE COUNT          624 a          827 c          791 g          693 t
ORIGIN      
        1 actgcaattt cgcggccggg aaatggcaca ccaggagctt ggcatgcaac tgatggaaaa
       61 agtgcgcgac gatctctcga gcatgtctca agtggaaatg gaaccaaaga tcaccggccg
      121 gaacgtaaca atgacgctct ccccgttacc ggcaaatcgc cgaaaaaggc gattcacttc
      181 gccgtcgaaa gcagccgacg aaggcgaacc tgctgtcttg ccggacggca cgtaattaca
      241 cgttggcgct ctgcgcgcac aagcggtgcc ggaacgtagg ccttttgcct gcgctggcag
      301 cggaatggaa atctgctgcg gcgcgcagac cacgagtcta ttccctgcaa aaagacgcaa
      361 gtcagggctt ctttgcaggt gtcggcgacg ctttcggcga agcggctggt atcggcgaaa
      421 ggggcagggg agtgtttatg gattgccgct tttccaatcg ctcttttcgg ctctttgcgt
      481 ctgcgttgcc cgggtccagc ttcagagctt gctccgtgtc ggccatggaa ctttttatgt
      541 cgcctttcgt ttcgtagatg taaccgcggt aaagatagta tcgagtttcg ttcggtttga
      601 gcttgatggc ttccgagtaa tcagcaagcg ccttatcata atcgtttgtt ttcatctcga
      661 ccgctgcccg ctgttcgtaa atccgcgcat cttctggctt gattttaatc gcttcgctgt
      721 agtcgctgat ggcatcgtta aattgctggt tagtcgcagc agcgtatcca cgttgttgat
      781 aaacagccgc gagttcatcc gcgtacttgc ggtcgagagc ggtggcttcg cgaagcaggt
      841 cgacggcctt gtcccactct tgatccctgg aggcttccgc gccttctcgg gcgagcttat
      901 ttgcttcctt ggcatccttg ctggcttgcg cctggcaaac ctgtgaggca caaagcgcca
      961 gagccgtgac tagagtaaaa gcacaaactt ttttcatgat tctcattaga cgttggtttt
     1021 tctacacaat tcgaaactca ggccgcgaat agctggccgc aagggcaact ctacgcctgt
     1081 tccggagaag gcacctgctc gatgtcgatc tttggttcgg gctccggcac cgccgcttcc
     1141 cccggcccag gcttatccac tcgcgtaatc cgcacagcac gcccggtcgt ttcgtcaatt
     1201 tcgatcaatg cgcccctcag ccgaacttcg ccagtagcga ccggaaatga tgctgggaga
     1261 tttgaaacga accggttcat gatcggttcg atggcccgcc cgagaatcga atttaccggg
     1321 ccgcacattc ccgcatcgca caaaaatgcg gtgcctccgg gaaagatctg ttcgtccgct
     1381 gtctgaacat gggtgtgagt cccgatcaca gcggatacct tgccatcgag aaatcgtccg
     1441 agtgcgattt tctcgctcgt ggtctcgcca tgcgcgtcca cgataatgtt tacagtttcc
     1501 cggcgcatct ttgccaccgc cgcttcgacc gcgtggaatg gattttccaa aatcggctgc
     1561 atgaatgtgc gtgcctgcac attgatcact ccgatttttc ccttggccgt ttccagaaca
     1621 atcgatccgc tgccaggcgc gccgtcagga taattcaatg ggcgaagcag acgcggttcc
     1681 aaagcgagga atgggacgat atctttttgg tcccaaatat ggtccccggt cgttacaacc
     1741 gaaacgcctg cgcgcaagag ctcgattgtg atttttcccg ttatgcctcg accgccggct
     1801 gcgttctcgc cgttaacgat aacgaaatcc aatgcatgct tttccttcag ttcgggcagc
     1861 cgggcgatga cgcctttacg ccctggctcg cctacgacat cgccgagaaa aagaaccgtt
     1921 agcattctgc gagcatcgta ttagagaggt ttcaaacgtc gatgtttcat ttcacaaaag
     1981 ttgcgcaagc agctcgccga tgtctggcaa atgacgaagc tcgaagcatt gcggctcgtc
     2041 attcgagctt cgtcattcct tcgtcattcg ccattcgagc ttcgtcattt ctcctgtcga
     2101 tcttcccgtt cgccctgcaa gcggccgata acatcagcat tctcggaagc aaaccgaaat
     2161 ggcaagtgct cgaacactat caggaaacaa ttacccatga tgagtttagt cgattgattc
     2221 aggacgttta ttgcacccac gggttcgctc cggatttaat cgaaataaac gagaagacgg
     2281 cgcgggtcct gatgaaccgc gagaagcaaa agtttttcac gttgcgcttc gcagaggacg
     2341 ccgcctcgcg cgacccggtg ccgcggcttt ggcgaccggc cgaatctctc ccgcccgcaa
     2401 aaccggaaaa gccgctctcc ggtttgcgga tcgcgctcga ccccggccac ctcggcggga
     2461 aatgggcaaa gatggaggag cgctggttcc aggtcgggag cagcgccccc gtgcaggagg
     2521 gcgatctcac tttgcgagtc gcgcgattgt tagcgccgcg attacgaaaa ttgggtgcgc
     2581 gagttttctt catccggaac agcaacgaac cggtcacggc aaaacgcccg gacgattttc
     2641 gggagctggc caggaaaatt ctgatcagaa acggtgtgcc tcaaccgctg cccgatgctc
     2701 ttgatccaaa cgatcccgaa aaagaacaaa cgattcgctg gcagagcgaa atcttgttct
     2761 accgttacag cgagattcga cgccgggcgg cgcgcatgaa cttcaaatta catcccgatc
     2821 tggtgctttg tttgcatttc aacgccgaag gctggggtga tccgaatagc ccaatgttgc
     2881 tcgacgcgaa tcatctgcat ctgctggtta acggttcgta tttgcaggac gagct
//
LOCUS       DASQUQ010000079         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_283963, whole genome shotgun sequence.
ACCESSION   DASQUQ010000079 DASQUQ010000000
VERSION     DASQUQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283963"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..>2524
                     /gene="purL"
                     /locus_tag="VE486_02235"
     CDS             346..>2524
                     /gene="purL"
                     /locus_tag="VE486_02235"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZA37937.1"
                     /translation="MTSQKPTIDPVVTPELVKQHGVTPDEFERIKKILGREPNFTELG
                     IFSVMWSEHCSYKNSRKELKKLPTTGPNILVKAGEENAGVVDIGDGWAIAFKIESHNH
                     PSAIEPFQGAATGVGGIIRDIFTMGARPEFCLNSLRFGPITKPVGRDSVEPTKSQSQV
                     ATNRRLFTGVVSGIAHYGNCIGVPTIGGEIYFDESFEGNPLVNVFCLGVLRHEQLARG
                     TAKGVGNPVFYVGAETGRDGLAGAAFASRELTEQSREDRPAVQVGDPFKEKLLLEACL
                     ELLARDAVTGIQDMGAAGLTCSTCETASRGGTGIEIDLARVPKREPGMTPYEILLSES
                     QERMLIIAKHGKENLVREIFAKWDVPCAEIGRVTDDGVMRVRDDGAIAAEIPAKPLAD
                     EAPLYSRESKPPSSVATVYDRRTNDLPEIDNHDALYQLLRDPTIASKNWVYRQYDHTV
                     RTGTMVKPGSDAAVFFVRYADKILAATTDCNSFYSALDPREGGKIAVAEAARNLTCSG
                     ARPLAVTDNLNFGNPYKPENFWQLREAVEGIAEACRAFGTPVTGGNVSLYNESPAGVV
                     DPTPTIAMVGLIDDEKHITTQWFEREDDAVILVGEIGNDLGGSQFLKICHGKKIGPSP
                     HVDLELEIKVQNAVRKLIQEGLAKSAHDCSEGGLAVALAECCFNPEKLLGAHIDLNAG
                     DTPATTALFNEAQSRIIISVASEDAERTMSILRERGVPFQQLGK"
BASE COUNT          576 a          703 c          712 g          533 t
ORIGIN      
        1 ctcataggtt gtaaccgtgc ccgcagaata cgtcgttgac gttgtcgtcg tcgtagcggg
       61 gtcggtcgtg gttgtttctt gggtcgttgt cgtgcttgtt tgcgcaaacg ccagcggcgc
      121 gatgagcgcg caggcgagag cgataattgt cttcttcact atattgcctc cctatttgtg
      181 tttgttaatg acttacccca aaccttcgca gctcggggac tgaacggacg cctcccagag
      241 tcgcccgtct gtcacatctt tgactaactc cgcgacgccg ctattcgaac taatttccga
      301 ttgtgtgatt gagcgcgcct ccggattcgt taacgtgacc tgctgatgac cagccaaaag
      361 ccgactatcg atccggtcgt tacgccggag cttgtcaaac agcacggtgt cactccggac
      421 gagtttgagc gcatcaaaaa aatcctcggc cgagagccga atttcaccga gctaggcatc
      481 ttctcggtga tgtggagcga gcactgctcc tataagaatt cgagaaaaga actgaaaaaa
      541 cttccgacca ccgggccaaa tattctggtc aaagccggcg aagagaacgc cggcgtcgtg
      601 gatatcggcg acgggtgggc gattgcgttc aagatcgaga gccataacca tccaagcgcc
      661 atcgagccgt tccaaggcgc agccaccggt gtcgggggaa tcattcgcga tatcttcacg
      721 atgggagcgc ggccggaatt ctgcctcaac tcgctgcggt tcgggccgat taccaagccg
      781 gtagggcgag actctgtcga gccgacgaaa tcccaatcgc aagtcgcaac taatcgccgt
      841 ctcttcaccg gcgtcgtctc cggcattgcg cattacggaa actgcattgg tgtgcccacc
      901 atcggcggcg aaatctattt cgacgaaagt ttcgagggta atcccctggt aaatgttttc
      961 tgcctcggtg tgttgcgcca tgaacagctc gcgcgcggca cggcgaaagg ggtgggcaat
     1021 ccagtttttt atgttggcgc cgaaaccggc cgcgacggtc ttgcgggcgc ggcgttcgca
     1081 tcgcgcgaat tgaccgaaca atcgcgcgag gatcgccccg ctgtgcaggt aggcgaccca
     1141 ttcaaggaaa aattgctcct cgaggcgtgc ctcgaacttc tcgcgcgcga tgcggttacc
     1201 ggcattcaag acatgggcgc ggcgggttta acctgttcga cctgcgagac ggcaagccgc
     1261 ggcggaaccg gtatcgagat cgatttagcc agagtgccga agcgcgaacc cggcatgacg
     1321 ccatacgaaa ttctgctcag cgaatcgcag gagcgaatgc tgatcatcgc caaacacgga
     1381 aaagaaaatc tggtccgtga aatttttgca aaatgggatg tgccctgcgc ggaaatcgga
     1441 cgcgtgaccg acgatggcgt gatgcgggtg cgcgacgatg gcgcaatcgc cgccgaaatt
     1501 ccggcaaaac cgcttgctga tgaagcgcca ctctattcgc gcgaatcaaa acctccttcg
     1561 tctgtcgcga cggtctatga ccgtcgcact aatgacctac cggagatcga caatcacgat
     1621 gcgctttacc aattactacg cgatccaacc atcgcctcaa agaactgggt ttatcgccaa
     1681 tacgatcaca ctgtgcgcac cggtacgatg gtaaagccgg ggtcggacgc cgccgtattt
     1741 ttcgttcgtt acgcggacaa aattctggcc gctactacgg attgcaattc attctattcc
     1801 gcgcttgatc cgcgcgaggg tggtaaaatc gccgttgccg aagccgcgag aaatctcacc
     1861 tgctccgggg caagacccct ggctgtgact gacaacctaa atttcggcaa tccctataag
     1921 cctgagaatt tttggcaatt gcgcgaagcg gtggaaggaa tcgcagaagc ctgccgcgca
     1981 ttcggcacgc ccgtaactgg cgggaatgtc tctttatata atgagagtcc cgcgggggtt
     2041 gtcgatccga cgccgaccat cgcaatggtc ggtctcattg acgacgaaaa acacatcacc
     2101 acgcaatggt tcgaacgaga ggacgacgcc gtcatcctgg ttggtgaaat cggaaacgat
     2161 cttggcggat cgcagtttct aaaaatctgc cacggaaaga aaatcgggcc gtcgccacat
     2221 gttgatctcg agcttgaaat caaggtgcaa aacgccgtgc gcaaattgat ccaggaaggc
     2281 ctggcaaaaa gcgcgcacga ttgcagcgaa ggcgggctcg cggtcgcgct tgccgaatgc
     2341 tgctttaatc ctgaaaaact tctcggtgca catatcgatc taaatgcagg cgacacgcct
     2401 gccactacag cgctgttcaa cgaggcgcag tcccgcatta tcatttccgt cgcttcggaa
     2461 gacgcagaga ggaccatgtc gatcttgcgc gagcgcgggg ttccctttca acagctgggg
     2521 aaag
//
LOCUS       DASQUQ010000080         7006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1012209, whole genome shotgun sequence.
ACCESSION   DASQUQ010000080 DASQUQ010000000
VERSION     DASQUQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012209"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VE486_02240"
     CDS             complement(<1..601)
                     /locus_tag="VE486_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37938.1"
                     /translation="MAKNSSSQSGSFNLRVVITVLLCAFSASLGWLSFAASPSGGTIT
                     PSGPTLTWTGTGNGVPPAAGGESDCEQGANCDAFKLTISGTPADWVAAGKQVKVRIVW
                     LLNSSDYDLYVHKGSLDGPVIASSGAGGTTFEEVTLNPNSSNIGTGDFFVNVVYFAAT
                     QADQYNGSAAVVAAGAPPIPAPTPASGVAPRYQNHTPPAA"
     gene            763..1413
                     /locus_tag="VE486_02245"
     CDS             763..1413
                     /locus_tag="VE486_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZA37939.1"
                     /translation="MVAELQEAHLHPQTRDALSSRERSREAAKSLFGQKGYESTSVSE
                     IARLAGTSESQVIHHFGGKAALLRAIFEDAWEQINRAIRLAIAKSRSPLDKLTLCFNI
                     VLAALEKDAGLGTLLLLEGPRLRRGQHMIVLTQGYQEFIEILDGILKEIADRGELAEQ
                     IKPEAFRSGIIGLVEGMLRHQLLARTSGFPASFSEADIWTLVSTLLSACLSSERRK"
     gene            complement(1728..2297)
                     /locus_tag="VE486_02250"
     CDS             complement(1728..2297)
                     /locus_tag="VE486_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37940.1"
                     /translation="MRSLSFVCLSVSLSLILSGCRERGSTIATRDACSLLSKAEVESV
                     QEAPIKETKSSKRSDGLFLVCQCFYTATEFSKSVNLALVQKDSNQRNERSPKDFWKEK
                     FGPYNTNDKERKGQENSKRGEEKEKGIPPKKISGLGDDAYWISNRFGGVLYVLKGDAF
                     VSISLGGPDNEEAKVNKSKALAEKALQRL"
     gene            2431..5166
                     /gene="ileS"
                     /locus_tag="VE486_02255"
     CDS             2431..5166
                     /gene="ileS"
                     /locus_tag="VE486_02255"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZA37941.1"
                     /translation="MSQNYKDTLNLPRTDFPMKANLAGREPEMLKMWEETRIYEQIQK
                     AREDRELFVLHDGPPFANGDVHMGTALNKILKDFVVKSQTMLGKRAPYVPGWDCHGLP
                     IEYKVVKESRGLSPLEVRKKSEAFARKYVNIQREQFKRLGVFGDWENPYLTMDPKYEA
                     EILRAFAVFVEKGLVYQSKKPVFWSTGAQTALAEAEVEYQERDDTAVYVKFPIVSGEW
                     KGKASIAIWTTTPWTLPANLAIAVDPKENYVVQQFQRDGASETLLLAEKLVPQFHANT
                     KFEPVGEPLESFPGSKIEKVTARHPFLDRDSTVFTASFVTMDTGTGCVHIAPGHGEDD
                     YLLGREKGLPILSPVDDHGRFTDEAGLPNLVGKYVFDANKDIVELLRERGMLLGEQKF
                     HHSYPYCWRSKTPIIFRNVEQFFIRIDELRSKALHAVKTVKWIPSWGENRISGTIESR
                     PDWVISRQRSWGVPLPVFYSKDGKAILDAKTVRKLADLVAERGSNIWFELNDADLASA
                     LGLPEGTTHRNDTIDVWIDSGVSHKAVCALHPELRDPADMYLEATDQHRGWFQSSLMT
                     SVALNNRAPYKICVTHGFVVDLDGKKISKSGTYDKPMAADHFVGRHGADLVRLWASSI
                     DYTDDVPFSEEMFTRLGDTYRRIRNTLRILLGNLYDFPVAGVADPGPRSTPAAATLID
                     RWILERLDDVIATCRTAYEAYEFHKVYHTLNQFCAVDLSSLYIDITKDRMYCDGPDAS
                     RRRATQAAMHTIFDALCRLLAPILTFTAEEAWRYSEAGGSVHLQEFPEPHDWHDKASA
                     QVAELLRLRGVIGQAIERARQEKLIGNALEAAVVLHSDSDVTKKIGKEELEEFFILSD
                     LTIHQTKDASASVTKTQYKKCARCWRHRPTVGASRVHPDLCDRCEDVVNKIAKSE"
     gene            5298..5783
                     /gene="lspA"
                     /locus_tag="VE486_02260"
     CDS             5298..5783
                     /gene="lspA"
                     /locus_tag="VE486_02260"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZA37942.1"
                     /translation="MKFIVFLSLPLYALDQWTKQLVLQFISPDESRVVISNFFSLVNV
                     TNTGAAFGSFRNNNTFFIVISSVALLVVLVLLLRRRPVDVWRDVSLALLLAGILGNLT
                     DRLLYGHVIDFLLFDLHVPYAHPWPAFNVADSCISIAVVLFIIHSFWADKRPAARREE
                     L"
     gene            5856..>7006
                     /locus_tag="VE486_02265"
     CDS             5856..>7006
                     /locus_tag="VE486_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZA37943.1"
                     /translation="MKERALSPMEKTAREIAARLRENGHIAYFAGGCVRDIVRGETPK
                     DFDIATDAKPETVQKLFPRTYAVGAHFGVIIVLENGFQFEVATFRSDEAYIDGRHPSA
                     VHFSSPEEDAKRRDFTINGMFYDPVADEVIDFVGGRADIEAKLVRAIGDPDQRFAEDR
                     LRMLRAVRFATVLDYTIDKQTWDALIANAISINQISAERIRDELVRIFLSPNRVRGWD
                     LLDASGLMRAILPELNRMKGVLQPEQFHPEGDVFKHTRLMLQFLPGKVSVPLVFAVLL
                     HDVAKPVTATVDETGRIRFSGHDRAGAEMAHEIMRRLRFSGAEIEATVEMVRQHMVFK
                     DVPKMREAKLKRFMARPTFDEELELHRVDCQGSHRMLDNYEFLLRKREE"
BASE COUNT         1553 a         1902 c         1921 g         1630 t
ORIGIN      
        1 ccgccgctgg aggcgtgtgg ttctgatatc ggggagcaac tcctgacgcc ggcgtcggtg
       61 caggaatggg cggcgcgcca gccgcaacca ccgcggcgct gccgttgtac tgatccgctt
      121 gtgtggccgc gaaatagacg acgttaacga agaagtcgcc cgtgccgatg ttggaactgt
      181 ttgggttgag ggttacttct tcaaaggtgg tgccgccagc gccggaggag gcgatcaccg
      241 ggccatcgag cgagccttta tgcacgtaca agtcataatc gctcgaattc agcagccaca
      301 cgatccgtac cttgacctgc ttgcccgccg ccacccagtc cgccggcgtg ccggaaatgg
      361 tcagcttgaa ggcgtcgcag ttggcccctt gttcgcaatc tgactctccg cccgcggcag
      421 gcggaacacc attacccgtg ccggtccatg taagagtcgg gccggaaggc gtaatggtgc
      481 cgccggaggg cgacgctgcg aagctcagcc agccaagcga tgcgctaaac gcgcagagaa
      541 gcacagtgat tacaacacga agattaaacg aaccggattg tgacgaagaa ttttttgcca
      601 taaagaactt tgctgccccc cgctgcacct tgggccttgg taatgttgct atgagaaata
      661 gtcgtagtgc tcctgttgtc aactcgaata ttactgaaaa atccgtgcgc ggtgtggaaa
      721 atattagttg acttgcgtcg gttcgatcta acctagtttt gtatggtagc agaactccaa
      781 gaggcccatt tgcacccaca gacgcgcgac gctctttcaa gccgcgagcg ttcgcgagag
      841 gcggcgaaaa gcctattcgg tcagaaagga tatgaatcaa caagcgtatc tgaaatagcc
      901 cggctcgccg gcacaagcga gtcccaagtc attcaccact ttggcggtaa agcagccttg
      961 ctgcgagcta tcttcgaaga cgcctgggaa caaatcaacc gggccattcg cctggcgatt
     1021 gcgaaaagcc gttccccact cgataagttg acgctctgct tcaacatcgt cctcgccgct
     1081 ttggaaaaag atgctggact cggtactctt ctcctacttg aaggccctcg gctccgccgc
     1141 ggtcagcaca tgatcgtgtt aactcagggc tatcaggagt ttatcgaaat cctcgacgga
     1201 atccttaagg aaatagccga tcgcggcgag ttggcggagc aaatcaagcc agaggcattt
     1261 cgttcaggaa tcattgggct ggttgagggc atgttgcgcc accaattgct ggctcgtaca
     1321 agcggttttc ctgcttcctt ctctgaagcg gacatttgga ctttggtttc tactctcctc
     1381 tcggcttgtc tgagcagtga acgccgaaag tgatttgctt ttcgcctcgg gccagtgcaa
     1441 gtctggtcag tcgcgactcc gctagtgtcg actctctaat ctcggtcgac cgtgtaacat
     1501 taatcgaaag ccatcgaatt tctttatgga aaaaaactcc gttcctgtaa ctgctcgtgt
     1561 cactatcggc gagctaggaa aacgagaact gctttttagg ccgcagggcc caagtccagt
     1621 tcgactattg ccagtctttg agtctctggt ccgtcctagg tccgggatag caaggactat
     1681 cggcacgcga ccagcaacca ggaggcacgc gactcccacc aaacgcgcta tagacgctgc
     1741 agggctttct cggccagtgc cttcgatttg ttgaccttgg cctcttcgtt atcgggtccg
     1801 ccgaggctga ttgagacgaa agcatcacct tttagaacat agagcacgcc tccgaatcga
     1861 ttgctgatcc aataggcatc gtccccgaga ccgctgattt tttttggcgg aattcccttc
     1921 tctttttcct cgccacgttt tgagttctct tgccctttgc gctccttgtc gtttgtgttg
     1981 tagggaccaa acttttcttt ccagaaatct tttggactgc gctcattccg ttgattggag
     2041 tccttctgga cgagcgccag atttactgat ttgctgaatt ccgttgctgt gtagaaacat
     2101 tggcaaacca ggaaaagtcc atcggaacgt ttgctgctct tcgtttcttt gataggcgct
     2161 tcctgaaccg attcgacttc cgctttggat aggaggctgc aggcgtcgcg cgtggctatc
     2221 gtgcttccgc gttccctgca cccgctgaga atcaatgaaa gggataccga gaggcagacg
     2281 aatgatagac tccgcatgaa agaactttcg gctaaccaac gccatcggac aacatgtctg
     2341 gtgacgatct cccgagagta tgtcgaccct aacatgggtg gtagttacgt tgcttcgcca
     2401 gttgacggat gttagcgtcg cctgccagcg atgagccaga actataaaga tactctgaat
     2461 ctgccgcgga cagattttcc gatgaaggcg aacctcgctg ggcgcgagcc ggaaatgctc
     2521 aaaatgtggg aagaaacgcg catctatgag caaatccaga aagcgagaga ggatcgagag
     2581 ctattcgtgc ttcatgatgg tccgccgttc gcgaatggcg acgttcatat gggcacggcg
     2641 ctgaacaaaa ttctcaagga ttttgtggtg aaatcgcaaa ccatgttggg gaagcgcgca
     2701 ccttacgtcc ccggctggga ctgccatggt ctgccgatcg aatacaaggt ggtcaaggaa
     2761 tcccgcggcc tttctccgct cgaagtccgc aaaaagtccg aagcgttcgc gcgcaagtac
     2821 gtgaatatcc agcgcgagca attcaagcga ctcggcgtct ttggcgattg ggaaaatcca
     2881 tatttaacga tggatcccaa atacgaagcc gaaattttgc gcgccttcgc cgtattcgtt
     2941 gaaaaaggtc tcgtctatca atcgaagaaa ccggtctttt ggagtaccgg cgcccagacc
     3001 gcattggctg aagccgaagt ggaatatcag gagcgggacg acaccgccgt ttacgtcaag
     3061 tttccaatcg taagcggcga gtggaaaggg aaggcaagca tcgccatttg gacaacgacg
     3121 ccctggacct tgccggcgaa cctggcgatc gcagtcgatc ccaaagaaaa ttacgttgtc
     3181 cagcagtttc agcgtgatgg cgcttccgag actctgttac tcgcagaaaa gctcgttccg
     3241 caatttcacg cgaacacgaa attcgagccg gtgggcgagc cgctggaatc attccctggc
     3301 agcaagatcg agaaggtaac tgcgcgacat ccctttcttg atcgcgattc cactgtcttc
     3361 acggccagct ttgtcacgat ggataccggc actggctgtg tccacatcgc gccgggccat
     3421 ggtgaagatg attacttgtt gggcagagaa aagggcttgc cgatcttgtc gccggtcgat
     3481 gatcacggcc gtttcaccga cgaagccggt ttgccaaatc ttgttggcaa atacgtcttc
     3541 gacgccaata aggacatcgt tgagttgctg cgcgagcgcg gtatgctgct cggcgaacag
     3601 aaattccatc attcgtatcc ctactgctgg cgttcgaaga cgccgatcat cttccgcaat
     3661 gtcgaacagt tcttcattcg catcgatgaa ctacgcagca aagctctcca tgccgtcaag
     3721 acagtgaagt ggattccgtc ctggggagaa aatcgcatca gcggaacaat tgaatcgcgt
     3781 cccgactggg tgatttcgcg tcaacggagc tggggcgtgc cgctcccggt cttctattca
     3841 aaggatggca aagcgattct cgacgcgaaa actgtccgca agctcgccga tctggtcgcg
     3901 gagcgcggct caaacatctg gtttgagttg aacgatgccg atcttgccag cgcgctcggt
     3961 ttaccggagg gcacaaccca tcgcaacgat acgatcgacg tttggatcga tagcggcgtt
     4021 tcacacaaag ccgtttgcgc gctgcaccct gagttgcgcg atccggctga catgtatctc
     4081 gaagcgaccg atcagcatcg cggctggttc caatcgtcgc tcatgaccag cgtcgctctc
     4141 aacaatcgcg ccccctacaa aatctgcgtg acgcacggct tcgttgtcga tcttgatgga
     4201 aagaagattt cgaaatcagg aacgtacgat aaaccaatgg ccgccgatca tttcgtcggt
     4261 cgtcatggcg ccgatctcgt tcggctctgg gcaagcagca tcgattacac ggacgacgtt
     4321 cccttttctg aagaaatgtt cacgcgcctc ggcgacacat accggcgaat cagaaacacg
     4381 ctgcgtattt tgctggggaa tctctacgat ttccctgtag ccggggtcgc tgaccccggc
     4441 ccgcggtcaa cgcccgcggc tgcaacgctg atcgatcgct ggattctgga gcggctcgat
     4501 gacgtcatcg caacatgtcg aactgcctac gaagcctacg agttccacaa ggtttatcac
     4561 acgctcaatc aattttgcgc ggtcgatttg agcagcctct acatcgacat caccaaagat
     4621 cggatgtatt gcgacgggcc ggacgcatca cgtcgccgcg cgacacaagc cgcgatgcac
     4681 acaattttcg atgcgctctg ccggttgctc gcgccgattc tcactttcac cgccgaggaa
     4741 gcgtggcggt attccgaagc gggtggctcg gttcacctgc aagaatttcc ggaaccgcac
     4801 gactggcatg ataaagcgag cgctcaggtt gcggagcttc tgagattgcg cggcgtgatc
     4861 gggcaggcga tcgagcgcgc gcgccaggaa aagctgatcg gaaatgctct ggaggcggcg
     4921 gtggttttgc attcggattc cgatgttacg aaaaaaatcg gtaaggagga attggaagaa
     4981 tttttcattc tgagcgatct gacaattcac cagacaaaag atgcgagcgc gtcggtcacg
     5041 aaaacgcagt ataaaaagtg cgcgcgttgc tggcgtcatc ggccgactgt tggtgcaagc
     5101 agggtgcatc cggacctatg cgatcgctgt gaggacgtag tgaacaaaat agcgaaatcc
     5161 gaatgacgaa agacgaaaga atgtcgaatg gcgagtaatc aagtcacagg cgtgagcgtc
     5221 atcgccattc gtgcttcgtc attgattcgt cattcgtcat ttgtcattcg tcattcccca
     5281 gggtcatagg atcgacaatg aagttcatcg tctttctctc gctcccgctc tacgcgctcg
     5341 atcaatggac aaagcagctg gtcctgcagt tcattagtcc cgacgaatct cgcgtggtga
     5401 tctcgaactt ttttagcctg gtcaacgtaa cgaacaccgg tgcagcattt ggttccttcc
     5461 ggaacaacaa cactttcttc atcgtaattt cgtctgtcgc gcttctcgtt gttcttgttc
     5521 ttctcctccg gcggcgacca gtagatgtct ggcgcgatgt gtcccttgcg ctcttgctgg
     5581 ctggcatcct gggaaatctt accgatcgcc ttctttatgg acatgtgatc gattttctcc
     5641 tcttcgattt gcacgttccc tatgcgcatc cctggcccgc gttcaatgtc gctgattcct
     5701 gtatctcgat tgccgtcgtc ttgtttatta ttcattcctt ctgggccgat aaaagacctg
     5761 ccgcgcgacg cgaggaattg tagggcaggc gcttcgtctg ccgtgtctgc gttattaagt
     5821 atgcggcggt cacagaccgc cgctacaata gatacatgaa agagagagct ctttcgccga
     5881 tggaaaagac ggcacgcgag attgccgcac gtttgaggga gaacggccat atcgcatact
     5941 tcgccggcgg ttgcgtgcgc gacatcgttc gtggcgaaac gccaaaggat ttcgacatcg
     6001 ccaccgacgc gaagccagaa actgtgcaga aactttttcc gcgcacctac gcggtcggtg
     6061 cgcattttgg cgtcatcatc gtgttggaaa acggtttcca gtttgaagtg gcgacgtttc
     6121 gttccgacga ggcgtatatc gacggaaggc accccagcgc agtgcatttt tcatctcctg
     6181 aagaagacgc gaaacgccgc gatttcacaa tcaacggcat gttttacgac ccggtcgctg
     6241 acgaagtaat cgatttcgtc ggcggccgtg ccgatatcga ggcaaagttg gtgcgcgcga
     6301 ttggcgatcc ggaccagcgt ttcgccgagg accggttgcg aatgctgcgg gctgttcgtt
     6361 ttgccaccgt gcttgattac acgatcgaca agcaaacatg ggacgcgctc atcgcgaacg
     6421 cgatctcaat caatcagatc agcgcggaac gaatccgtga cgagctggtg cgcatttttc
     6481 tctcgccaaa ccgggtccgc ggctgggatt tgctcgatgc gagcgggcta atgcgcgcga
     6541 ttttgcccga gctcaatcgg atgaaaggcg tcttgcagcc ggagcaattc catcccgagg
     6601 gcgacgtctt taaacacacg cgcttgatgc tgcaatttct gccgggaaaa gtttccgtgc
     6661 cgctcgtttt cgctgtgttg cttcatgacg tcgccaaacc tgttaccgcc accgtcgatg
     6721 aaacggggcg aatccgtttt agcggccatg atcgagccgg agcggaaatg gcgcacgaaa
     6781 tcatgcgccg tctgcggttt tccggcgccg agatcgaagc cacggtcgaa atggtgcgcc
     6841 agcatatggt tttcaaagac gtgcccaaaa tgcgggaggc aaaattaaag cgtttcatgg
     6901 cacgacccac tttcgatgag gagctcgagt tgcatcgggt cgattgccag ggcagccatc
     6961 gcatgcttga taattacgaa ttcctcctcc gcaaacgcga ggaatt
//
LOCUS       DASQUQ010000081         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_473860, whole genome shotgun sequence.
ACCESSION   DASQUQ010000081 DASQUQ010000000
VERSION     DASQUQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473860"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..460)
                     /locus_tag="VE486_02270"
     CDS             complement(77..460)
                     /locus_tag="VE486_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37944.1"
                     /translation="MKQSTEPVGESEVFWHLALRTDHLEKSLEAAINAGCEVTVPIRP
                     LDLMNNVTTAPWSVRVAFCFFDRRSLNRFDLLACNQPANIDLGLIRFPVGARDRLRRV
                     TFLTPRRNYEQRTPQQQDQNIPTHQ"
     gene            586..1191
                     /locus_tag="VE486_02275"
     CDS             586..1191
                     /locus_tag="VE486_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZA37945.1"
                     /translation="MADLAIGLPARRPTVVRQAGSLAAESAKLANIRFCIPCGKLTIN
                     EHYSTEEGSTVALRIFVLADTHNRLPEKVKEIAKDADEIWHLGDVCAETILDELRAIG
                     PRVTVVRGNCDSNFEWPLVIDLVRGGLKFRLQHVAPEKPPDDVDVLLHGHTHVPRNER
                     RGNVLFLNPGCVTRPNRGSPPSVAWLEIADGKIDWKMVPLR"
     gene            1300..2487
                     /gene="nuoH"
                     /locus_tag="VE486_02280"
     CDS             1300..2487
                     /gene="nuoH"
                     /locus_tag="VE486_02280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZA37946.1"
                     /translation="MSEAIDQIFVRIKQGLLAPLANAPDWLVQIVSSLINIFALLGVF
                     LTLFALMSVLERKILGRIQNRYGPNRVGPFGLFQPIADGIKMLIKEDIVPARADKIVH
                     FLAPVLIAATAILALGIIPYGRNMTPFTIDGGILFFFAVGSTTELAVFMAGWGSNNKF
                     SMLGAMRAIAQMISYELPLIITVLPVVMIVGSLNPDTIVAAQDGYAIGVVPRWFVFTP
                     WGAVAFILFFVSGLVESNRTPFDVPEGESEIVAGHMTEYSGFKYATFFMAEYIGMFAI
                     TGLATTLFLGGWHTPVRALEFVPSYLWFFGKLSALLFVYIWLRGTLPRTRVDQIMNFA
                     WKFMLPMAFTCIIAAAVWHYAGRGLRGWLWSLVVIAVAYTVLSILLDTRKKFAPRTYR
                     FAE"
     gene            2484..2963
                     /locus_tag="VE486_02285"
     CDS             2484..2963
                     /locus_tag="VE486_02285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZA37947.1"
                     /translation="MSASFLIISIFTLAAALAAATLPKLMHAALSFAVAFVGLAAFFF
                     LLGAEFVGLVQIFVYIGAVAVLIVFTILLTRRDVEKDRGFNWSGVVVAIAVFGGLIWA
                     IFKTQSLSLAAPVMPALTVKHIGEVLMTGYVWPLLCVGLLLTAALIGALILVMEEKQ"
     gene            3145..3453
                     /gene="nuoK"
                     /locus_tag="VE486_02290"
     CDS             3145..3453
                     /gene="nuoK"
                     /locus_tag="VE486_02290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZA37948.1"
                     /translation="MTLQLILIVSAAIFCIGLLAALSRRHAIFILIGIEMMLAAANLN
                     FVAFWRFSPDPHQPTGVMVAIFSIAIAAAEAAVGLALVISVYRHYRTTNVDELDKLRG
                     "
     gene            3428..>3772
                     /locus_tag="VE486_02295"
     CDS             3428..>3772
                     /locus_tag="VE486_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37949.1"
                     /translation="MNSTNSEDESGGDLNNRDRDHGSRIQRVNSWIVTNLWMIPAAPI
                     AISLVILSLAKSRRKSAAALAVTGQLMALTFSIFAFLPTLQTAGFRVVENFTWFTFGE
                     TALRIGWVLDPLA"
BASE COUNT          743 a         1070 c         1064 g          895 t
ORIGIN      
        1 ttcagggcaa caaaacccga aagtaacgtg aatcgtgaat tgccctcgaa tctcgcaggg
       61 ctctttgggg aatttattac tgatgcgttg gaatgttttg gtcctgctgt tgtggtgttc
      121 tctgttcata gtttctcctg ggtgtaagaa acgtgacacg cctcagccgg tcacgagcgc
      181 cgaccgggaa tcgaatcagg cccaaatcga tgtttgcggg ttgattacaa gccaggagat
      241 cgaagcgatt cagggatcgc cgatcaaaga aacaaaacgc aacgcgcacc gaccagggcg
      301 cagtcgtcac gttgttcatg agatccaatg gccggatcgg cacagtaact tcacagccgg
      361 cgttaattgc ggcctccagc gatttctcga gatgatcggt gcgcagggcc agatgccaga
      421 aaacctcgct ttcgccgact ggttccgtcg actgcttcat ttcgaccagc tcgatgtaca
      481 cgtcattccc ccgtttccag catcgcgaaa caaagcccat cgagcaggaa agggggcgac
      541 aaaaaccgtg cgtagcgaat gcaggataat cggatgtcag actgcttggc tgacttggcg
      601 atcgggctgc cagcccgccg tcctacggtt gttcgccagg ctggcagcct ggcggccgag
      661 tcagccaagc tggctaacat ccgattctgc attccttgcg gcaaactaac aataaacgaa
      721 cactactcca cggaggaagg atcgacagtg gcattgcgga tattcgtttt ggcggacaca
      781 cacaatcgtt tgccggaaaa ggtgaaggaa atcgccaaag acgcagatga aatctggcat
      841 cttggagatg tctgcgcgga aactattctc gatgagcttc gcgcgatcgg accacgagtc
      901 actgtcgttc gcggaaattg cgatagcaat ttcgaatggc cgctggttat cgatctcgtg
      961 cgcggtggac taaaattccg gctgcaacac gtcgcacctg aaaaaccgcc ggacgatgtg
     1021 gatgttctct tgcacgggca cacgcacgtc ccgcgaaatg agagacgcgg gaatgttctg
     1081 tttctcaatc ccggatgcgt gacccggcca aaccgcggct ctccgccaag tgtggcgtgg
     1141 ctggaaatcg cggatgggaa aatcgattgg aagatggtcc cgcttcgctg acggatgcgt
     1201 tgggggtcgc acgagctcgc ctctccgcaa cgcggcggcg gaaattggag aaataatctg
     1261 ccttgattaa acaacccggc atcgcttttc ttcatcaaag tgagcgaggc catcgatcag
     1321 atcttcgtcc ggataaagca ggggctgctc gcgccgcttg ccaatgcccc cgactggctt
     1381 gtccagatcg tatcgagtct gatcaacatt tttgcgctcc tcggcgtgtt tctcacgctc
     1441 tttgcgctca tgtccgtgct ggagcggaaa atcctcggac ggatacagaa tcgctatggc
     1501 ccgaatcggg tcgggccatt cggccttttt caaccaatcg ctgacggcat caagatgctg
     1561 atcaaggaag acattgtccc ggcccgcgcc gataagatcg tgcattttct cgcgccagtc
     1621 ctgattgccg ctaccgcgat tctcgccctc ggcatcattc cctatggccg gaacatgacg
     1681 ccgttcacga tcgatggcgg gatcctcttt ttctttgccg ttggctcaac aacggaactc
     1741 gccgtgttca tggccggctg gggcagtaac aacaagtttt cgatgttggg cgccatgcgg
     1801 gcgatcgcgc agatgatcag ctacgagctg ccgctgatta tcacagtgct gccggtggtg
     1861 atgatcgtcg ggtcgctcaa tccggacacg attgttgcgg cgcaagacgg ctacgcaatt
     1921 ggcgttgtgc cgcgctggtt cgttttcacg ccgtggggcg cggtcgcctt catcttattt
     1981 tttgtctccg gtctggtgga atcgaaccgc actccattcg acgtgcccga aggcgaatcg
     2041 gaaattgtcg ccggacacat gaccgaatat tccggtttca agtacgccac gtttttcatg
     2101 gcggaataca ttgggatgtt cgccatcact gggttggcga cgacgttatt tctcggcggc
     2161 tggcatacgc cggtccgcgc gctcgaattc gttccctcgt acctctggtt cttcgggaaa
     2221 cttagcgcgc tgctcttcgt ttacatctgg cttcgcggaa cgttgcctcg aacgcgcgtc
     2281 gatcagatca tgaacttcgc gtggaaattc atgctgccga tggcgttcac gtgcatcatc
     2341 gccgccgccg tttggcatta tgccggtcga ggtctccgcg gatggctttg gtcgctggta
     2401 gtaattgcag ttgcctacac cgtcctctcg atcctcctcg acacgcgaaa gaagtttgcg
     2461 ccgcgcactt atcgctttgc tgaatgagcg cctcattcct gatcatctcg atcttcacgc
     2521 tcgccgccgc gcttgccgct gcgaccttgc caaagctgat gcacgccgcg ctcagtttcg
     2581 ccgtcgcatt cgtcggcctg gccgctttct tctttttgct cggcgcggag ttcgtcggcc
     2641 tggtgcaaat cttcgtttac atcggcgcgg tcgcagtcct gatcgtgttc acgattctac
     2701 taacgcggcg tgatgtcgaa aaagatcgcg gcttcaactg gagcggtgtt gtcgtcgcga
     2761 tcgccgtatt cggcgggttg atctgggcga ttttcaagac gcaatccctc tctctcgctg
     2821 cgccggtaat gccggcgtta actgttaagc atatcggtga agtgttgatg accggttacg
     2881 tctggccttt gctgtgcgtc gggttgctcc ttactgccgc gctgatcggc gctctcattc
     2941 tcgtgatgga ggaaaagcag tgaagcgcga aggtctttgt ctggggaaca gaggctgcca
     3001 gcctgtagca ttcggcggcc tcgccgaatg cgtctttaca caacgtcgag ggtccgaaca
     3061 acgcgtggga gaggttcgcg gcaagctgct gcgaactaca ggccagcggc ctgtgctccc
     3121 cggaaacgga acggacgccg cgcaatgact ctccaactca ttctcatcgt atcggcagcg
     3181 atcttctgca tcggcttgct cgccgcgctc agccggcggc atgcgatttt cattttgatc
     3241 gggatcgaaa tgatgctcgc agcagcaaac ctgaatttcg ttgcgttctg gcgcttcagc
     3301 cccgacccgc atcaacccac cggggtaatg gtcgcaattt tttcgatcgc cattgccgct
     3361 gccgaagccg ccgtcgggct tgcgttggtc atctcggttt accggcacta tcgaacgact
     3421 aacgtcgatg aactcgacaa actcagagga tgagtccggc ggcgatctta acaaccggga
     3481 ccgggaccac ggatcccgaa tacaacgagt gaatagctgg atcgtcacta atctctggat
     3541 gattccggca gcgccgatcg ccatttcgct tgtcatattg agtttggcta aatcgcgccg
     3601 caaaagcgcg gcggcgcttg cagtcactgg ccagctaatg gcgctgacat tctcgatttt
     3661 cgcatttctg ccgacactgc aaacggcagg cttccgcgtc gttgagaatt tcacctggtt
     3721 tacgttcggc gagaccgcac ttcgcattgg ttgggtgctc gatccgctcg cc
//
LOCUS       DASQUQ010000082         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_651565, whole genome shotgun sequence.
ACCESSION   DASQUQ010000082 DASQUQ010000000
VERSION     DASQUQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651565"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VE486_02300"
     CDS             complement(<1..260)
                     /locus_tag="VE486_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37950.1"
                     /translation="MGRLVIASYCSSFLKSEMLHIYRQVKSLRGVDTFVVTKEVQNAQ
                     RFPFPDIEVIPKRRSNLLVHGWLKFVERRPPIVYRGEYQTLDS"
     gene            complement(305..1414)
                     /gene="obgE"
                     /locus_tag="VE486_02305"
     CDS             complement(305..1414)
                     /gene="obgE"
                     /locus_tag="VE486_02305"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZA37951.1"
                     /translation="MFVDRIKVFAQAGNGGRGCVSFRREKFVPKGGPDGGDGGRGGDV
                     VLRADQHVDNLSNLFYEPLIKAKNGGHGQGKKMHGRSAPQKIVKVPVGTIVWEAEEGK
                     SPALNAQRSTFSEEKDTIENQPIVDLTRDGQEFVLCHGGAGGKGNVHFKSSHNRAPRQ
                     YTEGEEGKQGHFLLELRTIADAALVGYPNAGKSTLLRKISAARPKVAAYPFTTLHPII
                     GVVEFPGYRRATIADIPGLIEGAHRGVGLGHEFLRHITRCQILLFVVDVAGSEGRNPT
                     EDLQNLRREIDLYDPILSKRPWYIIANKMDLPAARENLEALQNRFAQVEIAAVSAHTG
                     EGMDDLKVKLEKWLANASQRQPIDHAKINRTSSGN"
     gene            complement(1699..2904)
                     /gene="glgA"
                     /locus_tag="VE486_02310"
     CDS             complement(1699..2904)
                     /gene="glgA"
                     /locus_tag="VE486_02310"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HZA37952.1"
                     /translation="MMKVLFLTNEYPPHIYGGAGVHVGYLSRELAKSMPVEMRCFGDQ
                     RIEEGNLKVTGFELDTSDFTCPERLKSAFGAVRRCTDFNTTNIDADIVHCHTWYSHFG
                     GILAKKNYGIPLVITVHSLEPLRPWKREQLGGGYDFSLWVEKTALEMADAIIAVSGET
                     KRDIERLFDVGPERVHVIHNGIDPEEYCKVSSTDALKRHGIDPGKPYLLFVGRITRQK
                     GIIYLVRAIPYMHPDFQIVLCAGAPDTPDIAQEMKDAVESATRERSDVIWIEEMVDRN
                     AVIELYSHAAVFCCPSIYEPFGIINLEAMACETAVVASAVGGIKEVVVDGETGFLVPL
                     EQMKESPFEPLDPDKFSRDLAAKINQLMADPKLREKFGKAGRKRAEEKFSWSAIAEKT
                     KALYETLKH"
BASE COUNT          691 a          909 c          781 g          763 t
ORIGIN      
        1 gaatcgagcg tttgatactc accccgataa acaatcggcg gacgccgctc cacgaatttc
       61 agccaaccgt gcaccagcag attgctgcgc cgcttgggaa taacttcgat atcgggaaag
      121 ggaaatcgct gggcattctg gacctccttg gtcaccacga aggtgtcgac cccgcgaagc
      181 gactttactt gccggtaaat atgcaacatc tccgacttga gaaagctgga gcaatagctc
      241 gctatgacca atctacccat gacagagcgc ccagtttcgc tagggattcc gggcttgtcc
      301 acctttaatt gcccgaggaa gtcctgttga tcttggcatg gtcgatgggt tgccgttgcg
      361 acgcgtttgc caaccatttc tccaatttca cttttaaatc atccatgcct tcacctgtat
      421 gcgccgaaac cgcggcgatt tcgacctggg caaatcgatt ttgcagcgct tcaaggtttt
      481 ctcgcgccgc gggtaaatcc attttgtttg cgataatata ccatgggcgc tttgacaata
      541 ttggatcgta tagatcgatt tcgcgccgga ggttttgcag atcctcagtc ggattccgcc
      601 cttcgcttcc ggcaacatcg acgacgaaca acagaatttg gcagcgcgta atgtggcgca
      661 aaaattcgtg cccgagtcca acaccgcggt gcgcgccttc gatcaaaccg ggaatgtcgg
      721 cgatcgtcgc ccggcgatag ccggggaact ctaccacgcc gataatcgga tgcagcgtgg
      781 taaacggata cgctgccacc tttggacgcg ccgccgaaat tttgcggagc aacgtcgatt
      841 tgcccgcgtt cgggtatcca acgagtgctg catccgcgat cgtgcgcagt tccaggagaa
      901 aatgaccctg cttgccttct tcgccctcag tatattgccg aggcgcgcgg ttatgcgaac
      961 ttttaaaatg cacgttgccc ttcccgccgg cgccgccatg gcaaagaacg aattcctggc
     1021 catcccgcgt tagatcgacg atgggttgat tttcgattgt gtccttctct tcgctgaacg
     1081 ttgagcgttg agcgttgagc gctggggatt ttccctcttc cgcctcccat acaatcgttc
     1141 ctaccggcac cttcacgatc ttttgcggcg cggagcgccc atgcattttc ttcccttgcc
     1201 cgtggccgcc gttcttcgct ttgatcagcg gctcgtaaaa aagattcgat agattatcga
     1261 catgctgatc cgctcgcaga accacgtcgc caccgcgccc gccatcaccg ccatccggtc
     1321 cgccttttgg cacaaatttc tcccgccgga aacttacgca tccacgtcct ccattgcccg
     1381 cttgcgcgaa cactttgatt cgatcgacaa acatatcgcg ctaaaattgc cactggcacg
     1441 aacgcatgtc atcccgagtc gcgcagacga cgagggacct cacaatcgaa ccggccgtta
     1501 cgctggcaat tcagcgtaat caatcagcct tttgtgagat cattcgctcc gctcaggacg
     1561 accccgcgcc gaaaactgac gcgaccgcgc ctaccgtttc ctgcttgcgc aaacactttg
     1621 atttgatcgg caaacatggg gatgcggaag ttaaatcgtt aaaacgctta agcggcaaga
     1681 agcaccgacc cagacgcctt aatgcttcaa agtttcataa agcgccttcg ttttctccgc
     1741 gattgcggac cagctaaatt tttcttcggc gcgtttgcgc ccggctttgc caaatttctc
     1801 acgcagcttt ggatcagcca tgagttggtt aatttttgcg gctagatcgc gcgaaaattt
     1861 gtctggatcg agcggttcga atgggctctc tttcatttgc tccagcggaa ccaggaaacc
     1921 cgtctcaccg tccacaacga cttccttaat tccgccgact gcgctcgcca ccacggccgt
     1981 ttcgcaagcc atcgcttcaa gattgatgat gccgaacggc tcgtaaatcg aggggcaaca
     2041 aaacacagcg gcgtgcgaat aaagttctat caccgcattc cggtcgacca tctcttcgat
     2101 ccagatcacg tcagagcgct ctctcgtcgc gctctcgacg gcatctttca tttcctgggc
     2161 aatgtcaggc gtgtccggcg cgcctgcgca aagcacaatt tgaaaatctg ggtgcatata
     2221 tggaatggcg cggacaagat agataatccc tttttggcgc gtaattcggc caacgaagag
     2281 caagtacggt ttgccggggt caattccgtg gcgcttgagt gcgtcagttg aactgacttt
     2341 gcagtattcc tccggatcga tgccgttatg aatcacgtgc acacgctctg ggccgacatc
     2401 gaacaagcgt tcgatgtcgc gcttggtttc acccgaaacg gcgatgatgg catcggccat
     2461 ttccagagca gtcttctcaa cccatagcga aaaatcgtat ccaccaccga gctgctcgcg
     2521 tttccatggt cgaagcggtt cgagcgaatg cactgtgatc acgagtggaa ttccgtaatt
     2581 ctttttcgcc agaattccgc cgaaatggct gtaccacgta tggcaatgca cgatatcggc
     2641 atcgatattt gtcgtgttaa aatcggtgca acggcgcacc gcgccgaaag ctgacttcaa
     2701 tcgttccggg caggtgaaat cactcgtgtc caactcgaag ccggtaactt ttagatttcc
     2761 ttcctcgatc cgctgatcgc caaaacaccg catttcgacc ggcatcgatt tagccagctc
     2821 acgtgagaga taaccaacat gcacgccggc gcctccgtag atgtgcggcg gatattcgtt
     2881 ggtgagaaag aggaccttca tcattggagt gatggagtag tggaataatg gagtcttggt
     2941 caaacccgtt tgctcaacat cgactgatca ttactccatc actccagtac tccatcaatt
     3001 cttcactttc agcagcaaca caggcacatc gacttcgtgg cgcactttat cggccgtcgc
     3061 gccgtagaga atgtcgctga tgaaccggtg gccgtgcgtg gtcatggcaa tcagatcaac
     3121 ttctttctcg cgcgcgagtt tgat
//
LOCUS       DASQUQ010000083         8774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1093979, whole genome shotgun sequence.
ACCESSION   DASQUQ010000083 DASQUQ010000000
VERSION     DASQUQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093979"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VE486_02315"
     CDS             complement(<1..294)
                     /locus_tag="VE486_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZA37953.1"
                     /translation="MDVSGTIRAILNRKSGEIFSISPDATVFQAIEMMDKKNVGALLV
                     MEQDQLKGIISERDYTRKVILRGKRSRETKVAEIMSTDLTITHPREPIEKCLRL"
     gene            627..1580
                     /locus_tag="VE486_02320"
     CDS             627..1580
                     /locus_tag="VE486_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZA37954.1"
                     /translation="MQLAWPFKARAGNGRHEEEDRSPTGDAWPDFLCVGAQKGGTSWL
                     YRQCEPHADFWMPPLKELHYFNKLTKAKRVNPPRCRDDRDACFLESVNRLSALPYIDI
                     ENYARLFAYKGSRLSGDITPAYSMLNDEMIERVVSYFPNLKVIFLARDPVERAWSQLS
                     MGVRLGRIDRFDATDADAVIQKVLLPHVLSLSYPSKIVARWRRHVHPDLFRLYFFDDL
                     ERSPTELRRSIFQFLGADPDKPSGRVSADDNRDAGRQKLRLTDKVRSSLAQFFKDELR
                     ACAKELGGPAREWPARYGFSLLCFFIGLIDDDLLLWCDWLV"
     gene            1641..2618
                     /locus_tag="VE486_02325"
     CDS             1641..2618
                     /locus_tag="VE486_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZA37955.1"
                     /translation="MVRLAWPLKARVGDDGETEENGSSTGTSQPDFLCVGAQKGGTSW
                     LYRQLALHPDFWMPPIKEIHYFDKLSRNKRLSAPRCRDERDVCFMESLKSLSNRPSID
                     LERYGRLFMHKGSLLSGDISPGYSILSDEIIQRAVSYFPNLKVIFLARDPVERAWSQL
                     SMGVRLGRISRFDVTDIDEVIRNLRSPGVSSRSYPSKIVGRWRRHVHPDLFRLYFFDD
                     LERSPSELRRSIIHFLGADPNKPSEGLRADHNSSAGKEKLRLTDTVRSHVAQFFKDEL
                     RACAGELGGPAREWPARYGFSLLWFFIELADDIDLLVWCDWLVCAVANR"
     gene            complement(2623..3000)
                     /locus_tag="VE486_02330"
     CDS             complement(2623..3000)
                     /locus_tag="VE486_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZA37956.1"
                     /translation="MGTSSSGGSVMDAQCPSRLVLDRIADKWTALIIQVLANDTKRYA
                     ELQREIGGISQKMLTQTLRSLERDGLVQRKVHPVVPPKVEYSLTRLGRTLIEPLHALC
                     RWSEKHLAELQANRARAKANARE"
     gene            3123..4190
                     /locus_tag="VE486_02335"
     CDS             3123..4190
                     /locus_tag="VE486_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZA37957.1"
                     /translation="MKKNFVVFEEDEPAAVRLLNESPQPGNGSGFAGRNDIPSDYSLL
                     DEYSRTVVGAVERVAPAVANIEIQQPSKSHQRQIGGSGSGFVITPDGFILTNSHVVHG
                     AARIVVNLSDGRDYPAQLIGDDPETDLAVIRIDAPHLVHVRLADSESLRVGQIAIAIG
                     NPLGFQASVTSGVISALGRSMYSQSGRLIDNIIQTDAALNPGNSGGPLVNSAGEVIGV
                     NTAMIRPAQGICFAIASNTARLVAGWLIRDGRIRRSYIGVAGQNVPLHRRIIRFYNLP
                     LETGVLVVSVEKHSPAQGAGLREGDIIVGFDERPIGTIHDLHKMLVSEQIGVPAKMLV
                     IRHTEKLQLSVLPAESPVRAR"
     gene            4258..5547
                     /gene="purD"
                     /locus_tag="VE486_02340"
     CDS             4258..5547
                     /gene="purD"
                     /locus_tag="VE486_02340"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZA37958.1"
                     /translation="MKILIVGSGGREHALAWKLKQSPNAERIFCAPGNAGTAEIGENV
                     AISASDLASLVQFAKENRADLTVVGPDDPLAAGIVDLFTEEGLRIFGPTKTAARLESS
                     KIFAKQLMHEHGIPTAQAGTFEHSDDALRFCDRLQFPVVIKADGLALGKGVIIASDST
                     AARETIDEMMNQGRFGEAGRRIVVEEFLRGTECSLHALVDGANYRLLQTARDHKRALD
                     GDAGPNTGGMGAFSPANNWTTELEDQFHKKIMRPLLRGLQEKKITFRGLLYPGLMISA
                     DGARVLEFNCRFGDPETQAILPRMKSDLLPLLEATIDGKIDTSTIERDQRPAVTVVLA
                     SGGYPGKYETGKPISGLGEAAKIDGVQIFHAGTKRVNDEIVTAGGRVLAVTALGSTIE
                     AARARAYEALSHIDFENCHYRRDIALNAVRDNVGKLP"
     gene            5724..6689
                     /locus_tag="VE486_02345"
     CDS             5724..6689
                     /locus_tag="VE486_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZA37959.1"
                     /translation="MVDSLAPADLQAVITLLKSNFTNPDAITDTELNRATVEGLMARL
                     PRGVVLLPNKEGAPPEAPSAFYSEVLDGHIGYLRLGSLNGANLQAMDKSLGNFAAKKV
                     DALIVDLRSSQGTNDFAMAEDFARRFCPKGKPLFTLRKPAARQDRVFSSDRDPTFQGL
                     IMVLADGDTSGAAEAVASALRFYDKALVIGQPTAGRAAEYSDLPLPGGKILRVAVAEA
                     VSPEGRSLFPDGIRPDLPVEMQAANKRQIFQLSAEKGMGPFLYEAGRPHMNEAALLAG
                     TNPEVEAWGAAQQRRGRGPEKQPAHDLVLQRALDVVTSLEVYQKR"
     gene            6788..7489
                     /gene="rnc"
                     /locus_tag="VE486_02350"
     CDS             6788..7489
                     /gene="rnc"
                     /locus_tag="VE486_02350"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZA37960.1"
                     /translation="MSPIEERIGYKFRNSLLLAEALTHPSLAHETQRYHFDNQRLEFL
                     GDAVLQLVISEYLFRHFGGEAEGQLTKLRSRLVSRETLRMHAAALDLGRYLLMGRGEE
                     ASGGRERTSTLADAFEALIGAIYLDGGLDTAKEFILAQARSDLAQLAEEPIEINPKGH
                     LQELLQSISPRSPVYELISQSGPEHEKTFVVQAVWEGMVLGQGSGRSKKQAETAAALE
                     AMKLKHWEKNAEPDA"
     gene            complement(7668..8615)
                     /gene="thiD"
                     /locus_tag="VE486_02355"
     CDS             complement(7668..8615)
                     /gene="thiD"
                     /locus_tag="VE486_02355"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZA37961.1"
                     /translation="MSINNRRPRRKKRSAKKRWKVVRSKRSAATARKSLNRRIVKSLN
                     HHNQPPVALTIAGSDSSAGAGIQADLKTFSALGVYGLTAVTCVVAESPRRVSWIEAVS
                     AKVVRKQIEMLAESFPIAAIKTGLLYSPELVETVARALADLSGKSRKRLPLVIDPIIA
                     ATSGDRLLQPRAIKLYESKLFPLAMLITPNLDEAGKLLGQKIQDQQSMEKAARALARK
                     YRTSILLKGGHLAGDDAVDLLFSGGKIREFSSPFVRGVATHGTGCTYSAAITAGLASG
                     LLLEDAIGRAKKFVTKSIRDHFQWTSRSRGNLEALNHSP"
     gene            complement(8518..>8774)
                     /locus_tag="VE486_02360"
     CDS             complement(8518..>8774)
                     /locus_tag="VE486_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZA37962.1"
                     /translation="FTFTGKTMADAANKYAENVPGKFYVDDQCIDCDLCRETAPANFK
                     RNDDGGHSYVYKQPETPEEEALCKEAMEGCPVEAIGSDGA"
BASE COUNT         1900 a         2360 c         2532 g         1982 t
ORIGIN      
        1 gaggcgcaaa catttttcga tcggctcacg cggatgcgtg atggtcaaat cggtggacat
       61 gatctcggcg actttcgttt cgcgcgagcg tttgccgcgc aagatcactt tacgggtgta
      121 atcgcgttcc gaaatgatcc ctttcaattg gtcctgttcc atgaccagca gcgcgccaac
      181 gttcttctta tccatcattt cgattgcttg aaaaaccgtg gcgtccggcg agatggaaaa
      241 tatttcccca cttttgcgat tcaggatcgc gcggatggtg ccactgacgt ccatatgccc
      301 ggaagattta gctgccgaaa ctggcagttt cctagaatag gccgataccg ccctgcaatg
      361 caaaacaatt tgagggggtt tgatcaattg tcagatggga gcgcgggaga attggaaatc
      421 agcctaccag cctgactcgg ccggcgggca gcttgtctgc cgtgttcgct aggctggcag
      481 cctggcggcc gggtcagcca ggctggctga catccaactc cgcctccctt gaggcaaact
      541 aacaatcaac aaacactacc gtttggaggc gatttattcc ttttctgtcc ttgcttttcg
      601 ggcttcgagg ggaaaataaa gcagagatgc aattggcctg gccatttaaa gctcgcgccg
      661 gcaacggtcg ccatgaagaa gaggaccgct cgcccacggg cgatgcgtgg cccgacttct
      721 tgtgtgtggg agctcagaag ggcgggactt cgtggcttta tcgacaatgc gagccacatg
      781 ccgatttctg gatgcctccc ctgaaggaac tgcattattt taataaatta accaaggcta
      841 aacgcgtaaa cccaccgcgc tgcagagacg atcgcgacgc ttgctttctg gagagcgtaa
      901 atcgcctgag cgccctgccc tatatcgaca tcgaaaatta tgcgcgcttg ttcgcgtaca
      961 aagggtcgcg tctttctggc gacatcacgc cagcctattc gatgctgaat gatgagatga
     1021 ttgagcgagt cgtcagttat ttcccgaact tgaaagtgat ctttcttgct cgtgatccgg
     1081 ttgagagagc ctggtcgcaa ttgtccatgg gggtgcgtct cggaaggatt gatcgatttg
     1141 acgcgacaga cgccgacgca gtgattcaga aggtgttgct cccgcatgtc ctctcactct
     1201 cttatcccag caagatcgtc gcgcgatgga gacgccatgt tcatccagat ctgtttcgcc
     1261 tttatttttt cgatgatctg gagaggagtc ccactgagct gcgacgctcc atttttcagt
     1321 tcctgggcgc cgatccggat aagccaagcg gtcgagtcag cgccgatgac aaccgtgacg
     1381 caggcaggca aaagcttcga ttgacggaca aagtgcgctc cagtctggct cagtttttca
     1441 aggatgaact gcgggcttgt gcaaaggaac tgggcggtcc ggccagagaa tggccggcgc
     1501 gatatggctt ttcactgctc tgtttcttca taggactgat cgacgatgac ctgcttcttt
     1561 ggtgcgattg gctcgtctaa gccgcgaacg gtaattggct cgcgtccttg ctttttggag
     1621 ccaggaaaca aaataatgta atggtgcgtt tagcctggcc acttaaagct cgcgtcggcg
     1681 acgacggcga gacggaggag aatggatctt ctacgggcac gtctcaaccc gatttcctat
     1741 gtgtaggcgc ccagaaaggt ggaacgtcgt ggctttatcg ccaactcgcg ctgcatcccg
     1801 atttctggat gcctcccata aaggaaatac attacttcga taaattgagc aggaataaac
     1861 gattatctgc tccgcgttgc agagacgagc gcgatgtttg cttcatggaa agcctgaaaa
     1921 gcttgagcaa ccggccttct atcgacttgg agcgttacgg gcggttgttc atgcacaaag
     1981 ggtcgctcct ttccggtgat atttcgccag gctattcgat actgagcgac gagatcattc
     2041 agcgagccgt cagttatttc ccaaacttaa aggtgatttt tctggcgcgt gacccggttg
     2101 agagggcctg gtcgcagttg tccatgggag tacgcctcgg aagaattagt cggtttgacg
     2161 taacggatat cgacgaagtg attcggaatt tgcggagtcc aggggtctcc tcgcgctctt
     2221 atcccagcaa gatcgtcggg cgatggagac gccacgttca tccagatctg tttcgtcttt
     2281 attttttcga tgatctggag aggagtccca gtgaactccg gcgctccatc atccatttcc
     2341 tgggcgccga cccgaacaag ccaagcgagg gccttagggc tgatcacaac agcagcgcag
     2401 gcaaagaaaa gcttcgactg accgacacag tgcgctccca tgtggcgcag tttttcaagg
     2461 atgaactgcg ggcttgtgcg ggggaactgg gcggtcccgc cagggaatgg ccagcccgct
     2521 atggcttctc actgctttgg ttcttcatcg aactggccga cgatattgat ctgcttgttt
     2581 ggtgcgactg gctggtctgc gcggtggcca acaggtaatc catcactccc tggcattcgc
     2641 cttcgcccgc gcgcgatttg cctgaagttc ggccaaatgt ttttccgacc agcgacaaag
     2701 cgcgtggagc ggctcgatca aagtccgccc cagccttgtg agagaatact ccactttggg
     2761 cggcacgacc gggtgcacct ttcgttgcac aaggccgtcg cgctcaagac tgcgcagagt
     2821 ttgcgttagc attttttgcg aaatcccgcc gatctcgcgc tgaagctcgg cgtaacgctt
     2881 cgtatcgttg gccagcacct ggatgatgag cgcagtccat ttgtccgcga tgcggtcgag
     2941 cacaagccgg gacgggcact gcgcatccat gacggaaccg ccggaggaag atgttcccat
     3001 gtcctctttt taaagcgttc cacttccacg cgcaagaaga gagtggcagc ccaattaaac
     3061 tgtatagaag taaatcgctg tagttgacgc cgagtgacca cgtgtcgatc ttttagcggg
     3121 ttatgaagaa aaattttgtt gtctttgagg aagatgagcc ggctgccgtg cgcttgttga
     3181 atgagtcgcc gcagccggga aatggctctg ggtttgcagg cagaaacgat attccgagcg
     3241 actattcatt gctcgacgag tattcgcgca cggtggtggg cgcggtcgaa cgggtcgcgc
     3301 ccgcggtcgc gaacatcgag atccaacagc caagcaaaag ccaccagcgc caaatcgggg
     3361 gaagcggatc cggatttgtg attacgcctg atggcttcat tttgaccaac agccatgtcg
     3421 tccacggcgc tgcccgaatt gtggttaatc tctcggacgg ccgcgactat ccagcacagc
     3481 ttattggtga tgatcccgaa acggatctcg cggtgattcg gatcgatgcg ccccatcttg
     3541 ttcatgtgcg cctcgccgat tcggaaagtt tgcgcgtcgg acaaatcgca attgcaattg
     3601 gcaatccgct gggattccag gcgagcgtga cgtcgggggt aatcagcgcg cttggtcgct
     3661 caatgtattc gcaatcgggc cgtttgatcg acaatatcat tcagaccgac gcggcgctga
     3721 atccgggcaa ctccggcgga ccgcttgtca attcagccgg ggaggtcatc ggcgtaaaca
     3781 cggcgatgat tcgaccggcc cagggcatct gctttgcgat cgcgagcaac accgccaggc
     3841 ttgtcgccgg atggctgatt cgcgacggaa gaattcggcg cagttatatc ggagtcgccg
     3901 gtcagaatgt gccgctccat cgccgcatca tccgatttta taatttgccg ctggaaaccg
     3961 gggtgctggt tgtgtcggtg gaaaagcata gccccgcgca gggggccggt ctgcgcgagg
     4021 gcgacatcat tgttggtttc gatgagcggc cgatcggcac gattcacgat ctgcacaaaa
     4081 tgttggtcag cgaacaaatc ggcgttcccg cgaaaatgct ggtcattcgg cacaccgaaa
     4141 aattgcaatt gtcggtcttg ccagctgaat cacctgtccg cgcgcgataa ctgtagtgcg
     4201 gaaatccaaa agtttctcgc agccggcacg gctgtctcta cagtcaaccc ggttgctatg
     4261 aaaattctta tcgttggcag cggtggccgc gaacacgcgc tcgcatggaa attgaagcaa
     4321 tcgccgaacg cagagcgcat tttttgtgcg ccgggaaacg ccggcaccgc tgaaatcgga
     4381 gagaacgtgg cgatttcagc aagcgatctc gcatcactcg ttcagttcgc taaagaaaat
     4441 cgtgcggatc taacagtggt cggtccggac gaccctctcg ccgccgggat tgtcgatctg
     4501 tttactgagg aaggcttgcg catttttggg ccgaccaaaa cggccgcaag actcgagtcc
     4561 tccaaaattt tcgcgaaaca actgatgcat gagcacggga ttccaacggc acaggccggc
     4621 acatttgagc atagcgacga cgcgctccgc ttttgtgacc ggctccaatt ccccgttgtc
     4681 attaaagctg acggccttgc gctcggtaag ggagtaatca ttgcttccga ttctactgcc
     4741 gccagagaga caatcgacga gatgatgaat cagggccgtt ttggtgaggc gggccggcgc
     4801 attgtggtcg aggaattcct gcgcggcacg gaatgttcac tgcatgccct ggtcgatggc
     4861 gcgaactatc gcttgctcca aacggcgcgc gatcacaagc gcgcgctgga cggcgacgcc
     4921 gggcctaaca ccggcggaat gggcgcgttt agtccggcaa ataattggac caccgagctg
     4981 gaggatcaat ttcacaaaaa aatcatgcga ccgttgctgc gcggtttaca ggaaaagaag
     5041 attacattcc gcggcttgct ctatcctggg ttgatgatca gtgcggatgg cgcgcgggtg
     5101 ctcgagttca actgccgctt tggcgatccg gagacgcaag cgattttgcc gcgaatgaaa
     5161 tccgatttgt tgccgttgtt ggaagcgacg atcgacggca agatcgacac gtccacaatc
     5221 gagcgggatc aacggccggc agtcaccgtt gtgctcgcct cgggcggcta ccccggcaaa
     5281 tacgaaaccg gcaaacccat ctccggcctg ggcgaagcgg cgaagatcga tggggtgcaa
     5341 attttccacg ccggaacgaa gcgcgtgaat gatgaaattg tgacggccgg cggtcgcgtg
     5401 ctcgcggtca ccgcactggg atcaaccatc gaagccgcgc gggcgcgcgc ttacgaggct
     5461 ctctcccaca ttgatttcga gaattgccat taccggcgcg atattgctct gaacgccgtt
     5521 agggataacg tcggaaaatt accgtgatat ctcaattctc gctgacgact cttgcgatcg
     5581 cgatcttgag cgcgaatctc ttcgctcagt cgccgactcc ggcatcgccc gcgccaacgc
     5641 aatcggtgag acctccgcct gttgcatcga gcccgactgc ggctccatcg ccttcgccgg
     5701 caagaaagcc gacgacaaag caattggttg attcattggc accggcagat ttgcaggcag
     5761 taatcaccct cttgaaaagc aatttcacaa atcccgacgc aattaccgac acggaattga
     5821 accgggcgac ggtagagggt ctgatggctc ggttgccgcg tggcgtggtg cttttgccca
     5881 acaaggaggg tgcgccaccg gaagccccga gtgcctttta cagcgaggtt ttggacggtc
     5941 acatcggcta tctccgcctc gggtcgctga acggcgctaa tcttcaggcg atggataaaa
     6001 gccttgggaa ctttgccgcg aaaaaggtag atgcactgat tgtcgatctt cgctccagcc
     6061 agggaacgaa tgattttgca atggcggagg actttgcgag gcgattttgc ccaaaaggca
     6121 agccactctt cacactgcgc aaaccggccg cgcgtcagga ccgcgtgttt agttctgacc
     6181 gcgatccgac gtttcaaggg ctgatcatgg tgcttgccga tggcgacact tccggcgcag
     6241 ccgaagcagt cgcttccgcc ctgcgatttt atgataaggc cctcgtgatc ggccaaccga
     6301 ctgccggtcg cgcggccgaa tactccgatt tgcccttgcc tggcggcaaa attctacggg
     6361 tggcggtggc cgaagcagtt tcgccggagg ggcgttcatt atttccagat ggaataagac
     6421 cagatttgcc cgtcgagatg caggcggcaa acaagcggca gatttttcaa ttgagcgccg
     6481 agaaaggaat gggtccattc ctttacgaag caggacgtcc gcacatgaat gaggcggctc
     6541 tgttggcggg aacaaatcct gaggtcgagg cgtggggagc agcgcagcaa cgccgcggac
     6601 gtgggcccga gaaacaaccg gcccacgatc tggtcttgca gcgcgcactc gatgtcgtca
     6661 cctcgctcga ggtttatcag aagcgctagc actcgcgact gggctaacgg tcgcttcgca
     6721 ttctgttcga ttcctgcgcg tctggaaggt tgagagattc ttcgacttcg ctcagaatga
     6781 caggcacatg agtccgatcg aggaacgcat tggttataaa ttccggaatt cgcttttgct
     6841 ggcggaggcc ctcacgcatc ccagtctggc ccacgagacg caacgctatc attttgataa
     6901 tcagcgtttg gaatttctcg gggacgcggt ccttcagctt gtgattagcg aatatctctt
     6961 tcgccatttt ggcggcgagg cggagggaca actgacaaaa ttgcgttcac ggctcgtttc
     7021 ccgcgaaaca ttgaggatgc acgccgctgc tctggatttg gggcgttatc ttttgatggg
     7081 acgcggcgag gaagccagtg gcggtcgcga gcgaacgtcc acattggcgg acgcgtttga
     7141 agcgctcatt ggggctattt atctggatgg cggccttgat acggccaaag aattcatttt
     7201 agcgcaggcc cgctctgatc tcgcgcaact ggcggaagag ccgatcgaga tcaatccaaa
     7261 aggtcatttg caggaattac tgcaaagcat ttcgcctcgc agcccagtct atgaattaat
     7321 ttcgcaaagc gggcctgagc acgaaaagac atttgtcgtg caagccgttt gggaaggaat
     7381 ggttcttggc cagggcagcg gtcgcagcaa aaagcaggca gaaaccgccg ctgccctcga
     7441 agcgatgaag ctgaagcatt gggagaaaaa tgcggaaccc gatgcgtgag atcgaaagtc
     7501 cgcgcaattt cgctaagagc gcgaccgttg agtaacacag gcatcctgcc tgtttggtcg
     7561 agcggacttc cggcctgcgt tgccacctgc gggcaggatg cccgctcgac gaacaaccag
     7621 gatggttatg ttacgtctgc tgcggcgcgg ttcgcatagc gaaatggcta cggcgaatga
     7681 tttagcgcct caagatttcc gcgagaacgc gacgtccatt ggaaatgatc gcgaatcgac
     7741 ttcgtcacaa acttcttcgc gcgaccgatt gcatcttcca gcaacaggcc agaggcaagg
     7801 cccgcggtga ttgcggctga ataggtgcaa cctgtgccgt gggtggcgac accacgaaca
     7861 aacggcgacg aaaactcgcg aatcttccca ccactaaaga gtagatcgac tgcgtcgtcg
     7921 ccggccaggt gtccaccctt aagcaagatc gacgtccgat atttcctggc cagggcgcgc
     7981 gccgcttttt ccatcgattg ttgatcctgt attttttgac cgagcaattt tccggcttca
     8041 tccagattcg gcgtaatcaa cattgccagt ggaaacaatt tcgattcgta gagcttgatc
     8101 gccctgggtt gcaggagtcg atcaccgctg gtcgcggcga taatcggatc gatgacgagc
     8161 ggaaggcgtt tcctcgattt gcctgaaaga tcggcaaggg cgcgcgcaac cgtctcaacg
     8221 agctcgggag aataaagcag accagtctta atcgccgcga ttggaaaact ctccgccagc
     8281 atttcgattt gcttgcgcac aactttggcg ctgaccgcct caatccacga aactctgcgc
     8341 ggactttcgg caacgacgca ggtgaccgcg gtcaatccat aaacaccgag ggccgaaaac
     8401 gttttcagat cggcctgaat gccggcgcca gcgctgctgt ccgaccccgc gatggtcagc
     8461 gcgacgggcg gctgattatg atgatttagc gatttaacga ttcgacgatt taacgattta
     8521 cgcgccgtcg ctgccgatcg cttcgaccgg acaaccttcc atcgcttctt tgcagagcgc
     8581 ttcttcctcc ggggtctccg gttgtttata gacataggaa tggccgccgt cgtcgttgcg
     8641 tttgaaattg gcaggcgcgg tctcgcggca caaatcgcag tcgatgcatt ggtcatcgac
     8701 gtagaatttg cccggtacgt tttcggcgta tttgtttgcg gcgtctgcca tagtttttcc
     8761 ggtaaaagtg aagt
//
LOCUS       DASQUQ010000084         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_475891, whole genome shotgun sequence.
ACCESSION   DASQUQ010000084 DASQUQ010000000
VERSION     DASQUQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475891"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..927
                     /locus_tag="VE486_02365"
     CDS             145..927
                     /locus_tag="VE486_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZA37963.1"
                     /translation="MPLLEALPKLISWAIPLIILAVIILPQAIRILREYERGVIFRLG
                     KLLGAKGPGLIFLIPIVDRMVRMDLRVVTIDVARQEVMTRDNVPVSVDAVLYFRVVDP
                     QAAVVKVENFLKATSLIAQTTLRSVLGQAELDELLAHREKINQTLQEIIDRQTDPWGI
                     KVTAVEVKDVALPDTMKRAMAKQAEAERERRAKVVNAEGEFQAAEKMVQAAAMMSKEP
                     IALQLRFLQTMREISSEHNTTTFLPVPIDLFAPFKKSEPPKS"
     gene            1041..2672
                     /locus_tag="VE486_02370"
     CDS             1041..2672
                     /locus_tag="VE486_02370"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZA37964.1"
                     /translation="MTALDGELQKLIAFLDSPQSYPHRPAEVRVIQTHISWVFIASPF
                     VFKVKKPVNLGFLDFSTLEKRRYFCEREVELNRRLCPEIYLGVVPIYETDSGFSFKPL
                     GNIVEYAVKMKELPPGWFLSELLDKNLVSEKEIDRVISALHRFYQSETPTPEIEQWGT
                     REKLKISTDENFAQVKPFVDQTISSAAFETIRYFTNLFYAANEKLFCNRIQQHRILDC
                     HGDLHLDHIHLAPEATTIFDCIEFNDRFRFIDIANDLAFLAMDFDFEGRRDLGSLLLR
                     NAAREFGDPGMLKIANFYKCYRAFVRGKVESIQAAEPSSGIVEQALRLPTAEEHTTKA
                     TRYFRLALQYAVSGSEPLVLVIMGRVGAGKTTIAKQLATELGWPLFSSDQIRKTLAGV
                     PLTERTAAELRDKMYSEQMTEQTYKKLLDNGLASLATHNGVVLDATFSSPANRKSLRD
                     ACARAGVHLQAVELDVDPGEIKRRLKARDKSPSEISDARLEDFEKLSAAYEPPSELAP
                     ELFKISANGTVADTAKAVLLRLAEKQGGALSLGLP"
BASE COUNT          641 a          757 c          754 g          571 t
ORIGIN      
        1 gtgaaggtgc ggttctgatt tgcgcctcca gatctggcgg atagggctcg atccacggga
       61 tcggcgtcgg cgtcggaaaa caccaacggc actggcatac ccggcaatcc cgggtgaaac
      121 taagaaacaa aggagaatac agtcatgcca ttgctcgaag cacttccgaa attaattagt
      181 tgggccatac ccctcatcat tcttgcggtc attatcctgc cgcaggccat ccgcatcctg
      241 cgcgaatacg agcgcggcgt gatttttcgt ctcggaaagt tgctcggcgc gaaaggacca
      301 ggattgattt tcctgatccc gattgtcgat cgaatggtgc gaatggattt gcgcgtcgtg
      361 accattgatg tggctcggca ggaagtgatg acgcgcgata acgtgccagt cagcgtggat
      421 gcagtgctct actttcgcgt ggtcgatccg caggcggcgg tagtgaaggt ggagaatttt
      481 ctcaaagcca catcgcttat cgcccagacc acgttgcgta gcgtgctcgg tcaggcagag
      541 ctcgacgaac tactggctca ccgcgaaaaa attaatcaga cgctgcagga gatcattgac
      601 cgccagaccg acccgtgggg aatcaaggtc accgccgtcg aagtcaaaga cgttgcgctg
      661 cccgacacga tgaagcgcgc catggcgaaa caggccgagg ccgagcgcga gcgccgcgcc
      721 aaagtcgtca acgcagaagg cgaattccag gccgcggaaa aaatggtgca ggccgctgcg
      781 atgatgagca aggagccgat tgcgctgcaa ctacgcttcc tgcaaaccat gcgcgagatt
      841 tccagtgagc ataacaccac gacatttctc ccggtcccga tcgatttgtt cgcgccattt
      901 aagaagagcg aaccacccaa atcgtaggag ccggggcgct cacgtatgaa tctgtcatgt
      961 cgagcgaagt cgagacatcc cgttgtgcga gcgatcggta atttcgcggg attcctccac
     1021 tccgctgcac tccggtcgga atgacggccc tggatggcga attgcagaag ctgatcgctt
     1081 ttctcgactc ccctcaatcg tatccacatc ggccggcgga agtgcgcgta attcaaaccc
     1141 acatttcgtg ggtgttcatt gcgtcgcctt ttgtcttcaa agtgaagaag ccggtgaatc
     1201 tcggctttct cgatttcagc accctggaaa aacgccgcta tttttgcgaa cgcgaggttg
     1261 aattgaatcg gcggctctgt ccggaaattt atctcggcgt ggttccaatc tacgaaactg
     1321 attccggttt ttcattcaaa ccactgggaa acatcgttga gtacgccgtc aaaatgaagg
     1381 aactgccacc aggctggttc ttgagcgaat tgctcgataa gaatttggtc agtgaaaagg
     1441 agatcgatcg ggtgatctcc gctctccatc gattctacca gtctgaaact cccacgcctg
     1501 agatcgaaca atggggaacg cgagagaaac taaagatcag cacggacgag aatttcgcgc
     1561 aggtgaaacc gttcgtggac caaacgattt cgtcggccgc tttcgaaacg attcgctact
     1621 tcacgaacct gttctacgcc gccaacgaaa aattattttg caatcggatt cagcagcacc
     1681 ggattctgga ttgccacggc gacttgcacc tggaccacat ccaccttgcc ccggaagcga
     1741 cgactatctt cgattgcatc gagtttaacg accggtttcg gttcatcgac atcgccaacg
     1801 atctggcgtt tctggcgatg gatttcgatt tcgaaggaag acgcgacctc ggaagcctgc
     1861 ttctccgaaa tgccgcgcgc gaatttggcg accccgggat gttgaagatc gcgaactttt
     1921 acaaatgcta ccgggctttt gtgcgcggca aagtcgaaag catccaggcc gccgaaccct
     1981 catcaggaat tgtagagcag gcgctccgcc tgccgaccgc tgaggagcac acaacaaaag
     2041 cgacccgcta ttttcgcctc gcgctgcaat acgcggtgag cggctcggaa ccgctcgtcc
     2101 tcgtgataat gggccgggtc ggcgccggca aaacgacgat cgcgaagcag ctggccactg
     2161 aactgggctg gccgctcttt tcctccgacc aaattcgcaa aacactggcc ggcgtgccgc
     2221 taaccgagcg gaccgcagcg gagctgcgcg acaagatgta ttcggagcag atgaccgaac
     2281 aaacctacaa gaaacttttg gataacggac tcgcctcact cgcaacccac aatggtgttg
     2341 tgcttgatgc aaccttttca agcccggcaa accgcaaatc tttgcgcgat gcatgtgcta
     2401 gggccggcgt tcatttgcag gccgtcgaac tcgatgtcga tcctggcgag atcaagcgca
     2461 ggctgaaagc gcgagacaag tctcccagcg aaatctcaga cgcgcgtctt gaggactttg
     2521 agaaactgag cgcggcttac gagccgccat cagaattagc gcctgaattg tttaagattt
     2581 cagcaaacgg cacagttgcc gacactgcga aagcagtgct gttgcgtttg gccgagaagc
     2641 aaggaggggc gctttccctt ggcttgccgt agctcggcaa aggcggccaa gcccactcag
     2701 tcccagcggc gagcgtttct tga
//
LOCUS       DASQUQ010000085         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_925223, whole genome shotgun sequence.
ACCESSION   DASQUQ010000085 DASQUQ010000000
VERSION     DASQUQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925223"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VE486_02375"
     CDS             complement(<1..461)
                     /locus_tag="VE486_02375"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37965.1"
                     /translation="MDRSFCILMLVLASAVGVWAQGTPRRPVAQPAKPAAAPAATAPP
                     ATPQTVRLQFPNSDVVDVLHLYEQLTGNKLVMDNFVQGKVNIFIAKDVSREEAIKIIE
                     MSMGLNGISLVPAGVGIVEVVGAGQNPRKAAVPIVSELTDIPPGNPVISFL"
     gene            700..1221
                     /locus_tag="VE486_02380"
     CDS             700..1221
                     /locus_tag="VE486_02380"
                     /inference="COORDINATES: protein motif:HMM:NF022968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtzH-like domain-containing protein"
                     /protein_id="HZA37966.1"
                     /translation="MPPGDWRGRQSQHARSRALPGITRPRRVIAITFCILIAMTIFAA
                     PKQCAGGAIAEIRDVLQSQQDAWNNGDIDGFMNGYARSASTVFISEGTVRRGWETVRD
                     RYREKYSDRAKMGILTFSDLEITPLSPDSAVALGRWKLKRTQDQPHGRFTLIFRRFPE
                     GWRIVHDHTSAAK"
     gene            complement(1389..2780)
                     /gene="mpl"
                     /locus_tag="VE486_02385"
     CDS             complement(1389..2780)
                     /gene="mpl"
                     /locus_tag="VE486_02385"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZA37967.1"
                     /translation="MKEAPKKFHFIGICGTAMGSVAAALQERGFTVTGSDENIYPPMS
                     IFLEKKGIALKEGYRAENVPPDADVVVIGNAMKRGNPEVEAVLRRKLLYLSLPEVLRN
                     YFLRGRHNLVVTGTHGKTTTTALLVWIMEKAGRKPGYLIGGLPKNLGQGARLNDSKYF
                     VIEGDEYDSAFFDKRSKFIHYLPELLIVNNIDFDHADIFNNLDEIKLSFRRLLNIVPQ
                     NGMVLLNGDDPSCVEVARDCPAPMIEVGFSKNCAQRIREVAYSARGSRFKLGEDVFEV
                     PLIGDFNVRNAAMAAIAARFYDVPKAKIDNAFKSFSGIARRQEVRGEVRGVKVIDDFG
                     HHPTAIAHTLQALRQRYRGHRLWAVFEPRSNTTRRAVFQDQLPDALKLADGVLMAQVA
                     KLEQIPEKERLNPEAVVDAIAKAGHPAFYEKNAGAIVDRLVPLLKAQDVVVVFSNGGF
                     DNIHEKLLARLSS"
     gene            complement(2959..>4667)
                     /locus_tag="VE486_02390"
     CDS             complement(2959..>4667)
                     /locus_tag="VE486_02390"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZA37968.1"
                     /translation="FHAALDRYRDKVVLGANFDMENAAQAVTPNDALIPPPQLLDDRV
                     GFANFWSDPIDGKVRAVAYRVTDRQLAGLPPHASQEIYESLSARALTKIGHGNDVPRD
                     FRGHMIRFTGLDAFEPRPLYEVFDPKFWHANYADGAFFKDKIVMVGPSAQILHDFVDT
                     PMSPMTSGPSLNLQAMAAALGHEFLRPTPPKTGLALVGAAGLLAWFIIAFLRRPLLCL
                     GALIAIAVVYLGAARLFYDRSGLLLLTVPVLSALLLSGLFSLGFEYALERLEKLRTRR
                     TLERYVSKNLVKEVLENPGSYYSTLRGARVPVTILFSDLIGFTTLAEKADPEALVAQL
                     NEYLSRMTSVVFNNGGTLDKFIGDAIMAVWGNVRSLGVTQDAKNCARAGLGMRRELQQ
                     LNEKWREEGRMGLGMGVGINQGEVIVGNIGSQERMDPTVIGDSVNLASRLEGLTRIYG
                     VDILISATVAELVRDEFYLRSVARVQVKGKTKPVDVLTFIGARNEEVDPEFLKWLESY
                     EEGLEKFRSRDFTDAKILFSRFLEFYPDDVLAKMYLDRALEYERQPPNEAWDAVEVFK
                     KK"
BASE COUNT         1075 a         1353 c         1233 g         1006 t
ORIGIN      
        1 aacaagaaac tgataaccgg attgcccggc ggaatgtccg ttaactcaga aacaatcggc
       61 accgctgcct tgcgcgggtt ttgaccggct ccaactactt cgacaattcc gactcctgcc
      121 ggaaccaaag agattccgtt caagcccatg ctcatctcga tgattttgat tgcctcttcg
      181 cgagacacat ctttggcgat gaaaatgttg actttgccct gcacaaaatt gtccatcacc
      241 agtttgttgc cggtaagctg ctcgtaaagg tgcagcacat cgacgacatc gctgtttgga
      301 aattggagcc ggaccgtctg aggagtggcc ggaggcgcag ttgcagctgg tgctgcggcg
      361 ggcttcgctg gctgagcaac agggcgccga ggcgtccctt gcgcccatac gcccaccgcg
      421 ctcgcgagca cgagcatcag gatacaaaaa gatcgatcca cggtattccc tgtatgggcg
      481 aggatgctaa taaatttcgc tcgcgggtca aacgatttcg cgcgctcgga agtaactgac
      541 gtgtgccggg actggacgtg gcgagtgata ttctctaggt aattgttcat gttgcaaacg
      601 acaggctctt caacttcggg gagcgcacgc gccctcgcgt gcccttcgca gcgccctcgc
      661 cgcgaaccct cacatcgcaa agcggccccg atggcttcga tgcctcccgg cgattggcga
      721 gggcgccaat cgcagcacgc gaggtcgcgt gcgctccccg gaatcacgcg accccggcgc
      781 gtaatcgcga tcaccttttg tatcctgatc gcgatgacga ttttcgccgc gccaaaacaa
      841 tgcgccggtg gcgcaatcgc ggaaattcgc gacgtgcttc aatcgcaaca ggatgcctgg
      901 aacaatggcg acattgacgg attcatgaac ggatacgcgc gttcagcatc gaccgtcttt
      961 atttcagaag gcacggtgag acgcggatgg gaaacggtgc gcgatcgtta tcgagaaaaa
     1021 tattccgacc gcgcgaagat ggggatcctg actttttccg atctcgagat cacgccgctg
     1081 tcgccagatt cggccgtggc gcttggacgc tggaaattaa aacgcacgca agatcagccc
     1141 cacggaagat tcaccctcat cttcaggcgt tttcctgaag gatggcggat tgtgcacgat
     1201 cacacatcgg cggcgaagta accgcacact ttctcaacag cacccacgcg cgtcatcccg
     1261 agcgaagcga gggacctccc gcaacatgct gcgttacaca agcgaaactc gagtaacgca
     1321 tcacctaatg tgaggtcctt cgccgtcgga gcctgccccg agcgaacgta gtgagccaaa
     1381 cgggcggctc aggatgacaa gcgcgccagc aacttctcgt gaatgttatc gaacccgccg
     1441 ttgctgaaaa ccaccaccac gtcttgcgct ttcagtagcg gaacaagtcg atcgacaatc
     1501 gcaccggcgt tcttttcgta gaacgcagga tgacccgctt tggcgatcgc atccacaacg
     1561 gcctccggat tcaatcgctc cttttccggg atctgctcca gcttggcgac ttgcgccatc
     1621 aaaactccat cggccaattt tagcgcgtca ggaagctggt cttgaaaaac ggcgcgtcgt
     1681 gtcgtgttcg agcggggctc gaagacggcc cacaaccgat gaccacgata acgctgacga
     1741 agcgcttgta gggtgtgagc gattgccgtt ggatgatggc cgaaatcgtc gatcactttt
     1801 acgccgcgca cttcaccacg gacctcctgc cgccgggcga taccggaaaa acttttgaac
     1861 gcgttatcga tcttcgcttt tggcacatcg taaaaccgcg ccgcaatcgc tgccatcgcc
     1921 gcgttgcgca cattgaaatc gccgatcagc ggaacttcga aaacatcctc gcccagcttg
     1981 aaccttgagc ctcgagccga ataggcgacc tctcgaattc gctgggcgca gttctttgag
     2041 aagccgactt caatcattgg cgccgggcaa tctcttgcga cttccacgca actgggatcg
     2101 tcgccattga gcaagaccat gccattttga ggaacgatat taagcaaacg ccggaaactg
     2161 agcttgattt catcgaggtt gttgaagatg tcggcgtgat cgaagtcgat gttatttacg
     2221 atcaacaatt cggggagata atgaatgaac ttcgagcgct tatcgaaaaa agcggagtcg
     2281 tactcgtcgc cttcgatcac aaaatatttc gaatcgttca gacgcgcgcc ttgtccgaga
     2341 tttttcggga gtccgccaat gagataacca ggtttgcgtc cggccttttc catgatccag
     2401 acgagcagcg ccgtagtggt tgttttgcca tgcgtcccgg tgacaaccag attgtgccgg
     2461 ccgcgcagaa aataatttct caatacttcc ggcaacgaaa ggtaaagcag ttttcgcctg
     2521 agcaccgcct ccacttcggg attgccgcgc ttcatggcgt tgccgatcac cacgacgtca
     2581 gcatctggtg gaacattttc ggcgcgatag ccttccttca acgcgatccc cttcttctcc
     2641 aggaaaatcg acatcggcgg ataaatattt tcatccgaac cggtgaccgt gaagccgcgc
     2701 tcttgcaacg ctgccgcgac cgatcccatg gccgttccgc aaatcccgat gaaatgaaat
     2761 ttcttcggcg cttccttcac gcgactcttc tacgccagtc ggcagaaaaa gaaaccgcgg
     2821 attacacgga tttcacggat aggaagcgcc ctttgtcatt ccgaccaaag tggaggaatc
     2881 tcttattgtt taaggaagac catttagaga tgtctcgact ccgctcgaca tgacagaata
     2941 gcgaagcttg gattctcttt actttttctt aaacacttcc accgcgtccc atgcttcgtt
     3001 cggcggttgg cgctcgtatt ccagcgcgcg gtcaagatac attttggcca ggacatcgtc
     3061 cggataaaat tcgaggaaac gcgagaataa aatcttcgca tcagtgaaat cgcggctccg
     3121 aaacttctcc aaaccctctt cataggattc gagccacttt agaaattctg gatcgacctc
     3181 ttcatttcgg gcgccgatga aggtaagaac gtccaccggt ttggttttgc ctttcacttg
     3241 cacccgcgca accgaacgca gataaaattc gtcccgcacc aactcggcca cggtcgcact
     3301 aattaaaata tcgacgccat agattcgggt gagcccctcg agccgcgagg cgagattgac
     3361 cgagtcgcca atgacagtgg gatccatgcg ttcttgcgag ccgatattgc cgacaataac
     3421 ttcgccctga ttgatgccta cgcccatccc gagtcccatt cggccttctt cgcgccattt
     3481 ttcattgagc tgctgcaatt cccgtcgcat ccccagcccg gcgcgagcgc aatttttggc
     3541 atcctgcgtc accccaaggc tgcgcacgtt cccccagacg gccatgatcg cgtccccaat
     3601 gaatttatcg agcgtgccgc cgttgttgaa cacgaccgaa gtcatgcggc tgaggtattc
     3661 attaagttgt gcgaccaatg cttcgggatc ggctttctcg gctaatgtgg tgaagccgat
     3721 aagatccgag aatagaattg tcaccggcac gcgcgcacca cggagcgtgc tgtagtagct
     3781 gcccggattt tccagaactt ctttgaccag atttttcgac acgtagcgtt cgagcgtgcg
     3841 ccgggtgcgc aatttctcca accgctcgag ggcgtattcg aaaccgagcg aaaataaccc
     3901 gcttaataat agagcggaga ggaccgggac tgtcagaagc agcagccccg atctgtcata
     3961 aaacaatcgc gccgcgccaa gatacacgac ggcaattgca atgagggcgc ccaggcaaag
     4021 taacggccgg cgcagaaagg cgatgatgaa ccacgcgagc agccccgctg cgccaacaag
     4081 cgccaggcca gtcttcggtg gtgtgggtcg caaaaattcg tgcccaagcg ccgcggccat
     4141 tgcctgcaaa ttgagcgatg gccccgatgt cattgggctc atcggcgtat caacaaagtc
     4201 atgcaaaatc tgtgcagacg gtcccaccat cacgatcttg tctttaaaaa atgcgccatc
     4261 ggcataattg gcatgccaaa atttcggatc gaaaacttcg tagagcgggc gcggctcaaa
     4321 cgcgtcgagc cccgtaaagc gaatcatatg gccgcgaaaa tctcgcggga catcattccc
     4381 atggcctatt ttcgtcagag cgcgcgccga aagagactcg taaatttcct ggctcgcgtg
     4441 tggcggcaaa ccggccagct gccgatcggt gacgcgatat gcgaccgcgc gcacttttcc
     4501 gtcgatcggg tccgaccaaa aattcgcgaa cccgacgcga tcgtccagca gttgcggcgg
     4561 cggaatgagc gcatcgtttg gcgtaacggc ctgggcggca ttttccatat cgaaattggc
     4621 gccgagcacg actttgtcgc gataacgatc gagcgcggcg tgaaatg
//
LOCUS       DASQUQ010000086         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_834805, whole genome shotgun sequence.
ACCESSION   DASQUQ010000086 DASQUQ010000000
VERSION     DASQUQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834805"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..767
                     /locus_tag="VE486_02395"
     CDS             192..767
                     /locus_tag="VE486_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZA37969.1"
                     /translation="MIEKFFLAFIPIFVAIDPIGLVAVFMGLGTSASREQRKRQAFLG
                     ILTALCVAIGFIFLGKLIFSALGITVADFQVAGGLILLGLAGRELLGIGPHDRGGSDE
                     FGVVPLGLPLIAGPALLTALLILVDTVGLVFTLVSLLVNLALVAIAFYNADRFTRWMG
                     RQGLRGVSKIIALLLAAIAVSLIRRGWHAVG"
     gene            928..2217
                     /gene="hpnH"
                     /locus_tag="VE486_02400"
     CDS             928..2217
                     /gene="hpnH"
                     /locus_tag="VE486_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HZA37970.1"
                     /translation="MRFPLPLTAKIAAYMIGHKLRGTKKFATVLQLEPLHTCNLTCTG
                     CGRIREYSTSLKDMMSLEDCLGAAQECDAPMVSICGGEPLIYPHIEALVKGLLEQKRI
                     VYICTNAMFMRKKMREWLASQLAIQPEFVEAKIAALRGAGLISEKDAADIRKGPKDAA
                     KPTIGASDWMYWNVHLDGLERTHDLIVEREGVFKECILAIKMAKLLGYQVATNTTVYK
                     ETDMQEIEQMFDYLSDLGVDGHTITPGYEYDAAKKDMVKRLNLRPENFFLTRAMTVQK
                     FQKIEEWMNRYTFFGTPVYFEFLGGKRDLTCSAWAIPTRNIRGWKGPCYLMTDAHYSS
                     YAELLDKTEWERYGVVNGVARDSRCENCMVHCGYEPTATLGLQAQRGDTWKTVKFNFG
                     SKPKPTGRGSEVLAYNGVSSGNGHLTGKNAEASAKAG"
     gene            2248..2619
                     /locus_tag="VE486_02405"
     CDS             2248..2619
                     /locus_tag="VE486_02405"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZA37971.1"
                     /translation="MRTRHVYSFEKLRVWQEARRLVRNVYAVTKSFPRAELYGSTSQI
                     NRAAVSVAANLAEGSSRTSRRDQAHFSQIAYGSLMELACLLIVAVDLEFVSSEDKSKL
                     RDQIESISRQLNALRKIQLVP"
     gene            2659..>3009
                     /locus_tag="VE486_02410"
     CDS             2659..>3009
                     /locus_tag="VE486_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZA37972.1"
                     /translation="MNKSDVISLPSARPDLAQSYSASDAAISQAVSRAQENLLCQQKP
                     DGHWCGELIVDSTLCSDYVLFMHWCGEVDGQLQRRCVQHILKRQLLDGGWNIYHGGPS
                     EINASVKAYFALKLA"
BASE COUNT          680 a          757 c          853 g          719 t
ORIGIN      
        1 gccgcagcgg cgcgaaaatc atcgacggat tttagttcag cggcggaaag atcggcaatc
       61 agagtaaaag caagagtcag agagaggatc gacaaggaga agtatttcat gcggaaaggt
      121 ttggttgatg gagagagcga acattgtgta gaacgtttcc caggtaaagc gaaaacccgg
      181 gacataaatg catgattgag aaatttttcc tcgccttcat tccaatcttt gtcgccatcg
      241 atccgattgg gctggtggct gttttcatgg gcctgggtac cagcgcttca cgcgaacagc
      301 gaaaacgaca ggcgtttcta ggaatcttga ccgccctatg tgtagcgatc ggttttattt
      361 ttctggggaa acttattttt tcagcgcttg gaattaccgt ggccgatttt caggtcgcgg
      421 gtggtctgat tttgctcggt ctggctggtc gcgaattgct cggtatcggg ccacacgacc
      481 gcggcggcag cgacgaattc ggtgtggttc cgcttggcct gccgctcatc gcgggcccgg
      541 cgctgttgac ggctttgttg attttggtcg atacagtcgg ccttgtcttt acccttgttt
      601 cgcttcttgt gaatctggcg cttgtggcta tcgcgtttta caacgcggac cggtttaccc
      661 gctggatggg acgacagggt ttacgcggtg tgtctaaaat aatcgcgcta cttctcgcgg
      721 cgattgccgt cagtttgatc cggcgcggct ggcatgccgt gggttgattc gactagcgac
      781 cctgatctac tctgccttcg gccggcgatt ctaatttgtg gcaggctaaa tccaaacagc
      841 aggccagcgc cgattcaatc tagcaaatcg ggagcccaaa ctcggggcat tgacactctg
      901 gcaacgcttg ttactatcgt cggttttatg agatttcccc tcccgctcac agccaaaatc
      961 gcggcctaca tgatcgggca taagttgcgt ggcacgaaga agttcgccac ggtgcttcaa
     1021 ctcgagccgt tgcacacttg caacctcacc tgtaccggtt gtggacggat tcgcgaatac
     1081 tccacgtcgc tgaaggatat gatgagtctg gaggattgcc tcggggcggc gcaagagtgc
     1141 gatgcgccaa tggtctcgat ctgtggcggc gaaccgctga tttatccgca tatcgaagcc
     1201 cttgtgaaag gattgctcga gcaaaagcgg atcgtttaca tctgcacaaa cgccatgttc
     1261 atgcgcaaga aaatgcgcga atggcttgcg tcgcaactgg cgattcagcc ggagtttgtc
     1321 gaagcaaaaa tcgcggcatt gcgcggcgcg ggtttgatca gcgagaaaga tgcagcggac
     1381 attcgcaagg gcccaaagga tgccgccaag ccgacgatcg gagcgtcaga ctggatgtat
     1441 tggaacgtgc atctggacgg cctcgagcga acacacgatc tcattgtgga gcgcgaaggc
     1501 gtttttaagg agtgcatcct tgcaatcaaa atggccaagt tgctcggtta tcaggtcgcg
     1561 accaacacaa cggtttacaa ggaaaccgac atgcaggaga tcgagcagat gttcgattac
     1621 ctttccgatc ttggcgtgga tggccatacc attactcccg gatacgagta cgacgctgcc
     1681 aagaaagaca tggtcaagcg gctcaatttg cgtccggaga atttctttct cactcgcgcg
     1741 atgactgttc agaagttcca aaagatcgag gaatggatga atcgatacac attttttggg
     1801 acgccagttt attttgaatt tctcggcggc aagcgcgatt tgacgtgttc agcctgggca
     1861 attccgacac gaaacattcg cggctggaaa ggcccctgct atctcatgac cgacgcgcat
     1921 tattcgagct atgccgaact gctcgacaaa acggagtggg agcgatacgg ggtcgtgaac
     1981 ggggtggcgc gtgatagccg ctgcgagaac tgcatggtgc attgcggtta cgagccgact
     2041 gcaactttgg gtctgcaagc gcagcggggc gatacttgga aaacggtcaa attcaatttt
     2101 ggatcgaaac cgaaaccgac cggccgtggc agcgaagttc tcgcgtataa cggcgtgagc
     2161 tccggaaacg gacatctcac gggaaagaat gccgaggctt cagcgaaagc tggttgagcg
     2221 gttgagtggt taagaggatt ggcgattgtg agaacgcgac atgtctattc cttcgagaag
     2281 ctgcgagttt ggcaagaggc gcggcgactg gttagaaacg tttacgctgt aacgaagtct
     2341 tttcctcgcg ccgagcttta cgggtcaaca tctcaaatca atcgggcggc agtttctgtc
     2401 gccgccaatc ttgcggaagg atcttcgcgc acgagccggc gcgatcaggc ccatttttct
     2461 cagattgcct acggctcatt gatggaactt gcctgcttgt taattgttgc cgtcgatctc
     2521 gagttcgttt ccagcgaaga caaatcgaaa cttcgcgacc agatcgaatc catttcgcgc
     2581 caacttaatg ccctgcgaaa aatccagctg gtcccctgat ttctcttctc aactacttaa
     2641 ctactcaacc tttcaaccgt gaacaagtct gacgtaattt ctttaccaag cgcacggcct
     2701 gatctcgcgc agtcatattc cgccagcgac gccgcgattt cgcaggccgt cagtcgcgcc
     2761 caggaaaatc ttctgtgcca acagaagccc gacggtcatt ggtgcggcga gctgatcgtc
     2821 gatagtacat tgtgctccga ttacgtgcta ttcatgcact ggtgcggcga agtcgatggg
     2881 caattgcagc ggcgttgcgt ccaacacatt ttgaaacggc aattgctgga cggaggctgg
     2941 aacatctatc acggcggccc gagcgagatt aacgcttccg tgaaggcata tttcgcgttg
     3001 aaactcgct
//
LOCUS       DASQUQ010000087         4808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1185372, whole genome shotgun sequence.
ACCESSION   DASQUQ010000087 DASQUQ010000000
VERSION     DASQUQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185372"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..2061
                     /locus_tag="VE486_02415"
     CDS             328..2061
                     /locus_tag="VE486_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZA37973.1"
                     /translation="MHELIRDITYCTVAAWLLGLGAQFARQPLLLAYLAGGFALGPMG
                     FGFIQSAESISAISELGLIFLLFMIGLEIDLKKIASAGRSITLTALVQILGGIALGVI
                     FFRLCGFALGGGKWDALYLAVAAALSSTVIVVKMLYDKEELDTLTGRITLGVLVLQDL
                     AVILFLAIQPNLGNLAPGLLLLSLVRVTVLVVATFAVSRYVLPAIFRGVARVPELMQV
                     GAIAWCFLIGEFGQQLHLSREMGALVAGVALSTFPYALDVTAKVISLRDFFVTLFFVA
                     IGMQIPLPTGSLIGWVLTFAAFVVITRVATVFTPLYLMRQGLRTSFIPALNLCQISEF
                     SLVVLTLGAQAAHLASSTTHGIVALAFTLLAVVSTFSMSKSDSLARRLIPWLKKFGFR
                     DLDHVPADVYAAGAAAHDHSARIVFVGFFRTASSLLEELQRRYPELVAEVAVVDFSPV
                     ARDGLLKRQVPVIYGDVSHRDTLVHARVDKAEVLISTVPDSLLKGTTNARLVHLLREL
                     NPTAKIIATADVLAQARELLDAGADYVNVPRLRQAGDLLEAVRAARDGLLDEKRAALR
                     EILLDRCEVLA"
     gene            complement(2371..3183)
                     /locus_tag="VE486_02420"
     CDS             complement(2371..3183)
                     /locus_tag="VE486_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZA37974.1"
                     /translation="MASEAPIDSAPGSAVTAVVAPPERADEIFLEGPRSRFAEFCTLL
                     RVMRDFLRGYRALHFVGPCVTVFGSARIKPDDPYYEQALKMGAAIARLGFTVMTGGGP
                     GIMEAANRGAKEVGGRSVGCNIELPFEQSSNVYLDRCVRLHYFFVRKALLIKYSYAFV
                     VMPGGAGTLDELFEAVTLIQNGKIKNFPIVIMGTDYWKQLISFIEQMAKRGKISSSDL
                     NLIFATDSVEEAIAHIRTKAIERFGLKRVAHMRRPFPWLGERGFPGKGPEIC"
     gene            complement(3212..4645)
                     /gene="rpoN"
                     /locus_tag="VE486_02425"
     CDS             complement(3212..4645)
                     /gene="rpoN"
                     /locus_tag="VE486_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZA37975.1"
                     /translation="MPALGMQQRQSLALQQVLSPQLQQSLLILQTPLLELRNLVQQEM
                     ETNPVLEELPEDSGANERSEAEASADEKFKDEFEKLASLDEEWRDYMAQSASSNFDGR
                     RGSKEADEKRQFLFDSIPVQETLQQNLIAQLNQTVLSADDRKAAELIVGNIDDDGFLQ
                     STPEEMALNSGIPQDDFERGLGVIQAFYPAGVGARDLRECLLIQLQRQGKEHSLEHRI
                     VSEHMDDLGRHRFLEIARRMAIGVEDVQKAADNIARLNPRPGQVFAAAPQNYVLPDVI
                     VEKVDGEYQIAFNNEQIPHLRISNLYKDIISSGDSQSSDVKDYIRDKIRSGKFLIRSI
                     HQRQQTIMNIAQQIVSRQRDFLEHGPSHLKPTTMAEVAEAVGVHETTVSRAVSGKYMS
                     TPQGVFEMKYFFTGGYQTATGESLSNTSVKQAILDLVKHESGSAPLSDHEIVEILSER
                     GIPIARRTVAKYRSELNILPSHMRRKY"
BASE COUNT          947 a         1360 c         1329 g         1172 t
ORIGIN      
        1 ataaaaatga agagctttag tgcggacatt acggattcag aggccactcg gaaatgtctc
       61 ttcaccttct ccggcctctc attctgttgt cggctggcgc aaacaattcc tgcagccttt
      121 atacgaaatc cacccggtct aatccgatca taaggcggaa ttaataataa cgggggaaag
      181 atttccggac ggtgcgattt ccttgtgttt gcatcgcgca tcggcagact acgcaccata
      241 tgccaagtcg cgacgaccga atgtgaaagc ggtgggaatt ccgaatccgg tcgctttctg
      301 gccacgggaa tagcgggcga aatcaatatg cacgagctaa ttcgcgatat cacttattgc
      361 accgtggccg cgtggctgct tgggctcggt gcgcaatttg cccggcaacc attgcttctc
      421 gcttatctgg ccggcggctt tgcacttggt cccatgggat tcggctttat ccagtcggcc
      481 gaatcgatct cggcgatttc agagttggga ctgatttttc tcctattcat gattgggctg
      541 gagatcgatc tgaagaaaat cgccagtgcc ggccgctcca ttactctcac cgcgctggtg
      601 cagatcctcg gtggaatcgc gctcggcgta atttttttcc gtctgtgcgg gtttgctctc
      661 ggcggcggga aatgggacgc cctttatctg gccgttgcag ctgcgctcag cagcaccgtg
      721 atcgtagtca aaatgctcta cgacaaggag gaactcgaca cgctaaccgg acggatcacg
      781 cttggtgtac tcgtgcttca ggacctggcg gtcattctct ttctcgcgat ccagccaaat
      841 ctcggaaacc tggccccggg acttctgctt ctctcgttag tacgcgtgac cgttctggta
      901 gtcgcgacgt ttgcggtaag ccgatatgtg ttgccggcta tttttcgtgg cgtcgcgcgc
      961 gtgccggaac tgatgcaggt gggcgcgatc gcctggtgtt ttctcattgg cgaatttggc
     1021 cagcagttgc atctctcccg tgaaatgggc gcgctagtgg cgggggttgc gctctccacg
     1081 ttcccgtatg ccctcgacgt gaccgcaaag gtgatcagtc tgcgcgactt tttcgttact
     1141 ctctttttcg tggcgatcgg gatgcaaatt ccgttgccga ctggcagtct catcggctgg
     1201 gtgctgacgt tcgctgcctt tgttgtcata actcgagtgg cgactgtttt cacgccgctc
     1261 tacctgatgc gccagggctt gcggacgagc ttcatccctg cgttgaacct ttgccagatc
     1321 agcgaattct cgctggtggt gctcactctt ggcgcgcagg cagctcacct tgccagttcg
     1381 actacccacg gcatcgtcgc gctcgcattc actctccttg cagttgtttc cacctttagt
     1441 atgtcgaaat ccgacagtct ggcgcggagg ctcattccgt ggttgaaaaa atttggtttc
     1501 cgtgatctcg atcacgtgcc cgctgatgtc tatgcagccg gcgccgccgc ccacgaccac
     1561 agcgcccgca ttgtcttcgt tgggtttttt cggactgcca gttcgctgtt ggaagagcta
     1621 cagcgtcgtt accctgaatt ggtcgccgag gtcgcggtgg tggacttcag ccccgtggca
     1681 cgagacggcc ttctgaagcg acaggtgcct gtaatttatg gtgacgtctc gcaccgggat
     1741 acgcttgtcc atgctcgtgt ggataaagct gaggtgctta tttcgactgt tccagattcg
     1801 ctcctcaagg ggacaacaaa tgcgcggctg gttcaccttc tgcgcgaact gaaccccacc
     1861 gcaaagatca tcgccactgc cgacgtgctt gcacaagcga gggagctcct ggacgcaggc
     1921 gctgattacg taaacgttcc gcgtctgcgc caggctggcg atctgcttga agccgtgcgc
     1981 gctgccagag acgggttgct tgatgaaaag cgcgcggctc tacgcgaaat cctgctcgat
     2041 cgatgcgaag tgctcgcgta aacgcgtcat tccatgtgga atggccaatc cccgattcta
     2101 agatcagagc gatgcgtgcg cttgccatcc actttttatt cggcgtcagg gccgcagatg
     2161 tcctgtttga gccgatagag agaaaggttt tcgatcaaac agaagctttg cgagttcgcc
     2221 cgcgttgtta atacactacc ggtataaccg gttccagatc accgcgccga caagaccaaa
     2281 gatatatccg atcgcgctcg tggcctcttg cggaatcgcc gcatttttga ggcctacaag
     2341 tagcggcgat ctccagtcgg ccgttgggca ttaacaaatt tccggaccct tgccgggaaa
     2401 gccacgctcc ccgagccatg ggaaggggcg gcgcatgtgt gctacgcgtt taagcccaaa
     2461 tcgctcaatt gccttagtgc gaatgtgcgc gatggcttct tcgacagaat ccgtcgcgaa
     2521 aatcaggttt aaatccgacg agctgatttt cccgcgcttc gccatctgtt cgatgaaact
     2581 gattagttgc ttccagtaat cggttcccat gatcacaatc ggaaaatttt tgattttccc
     2641 gttctgaatc agcgttaccg cctcgaacaa ctcatcgagc gtgcctgcgc cgccgggcat
     2701 aacaacgaag gcgtaggaat attttatcag cagcgctttg cgaacgaaaa aataatgcag
     2761 ccggacacaa cgatcgagat acacgttgga tgattgctcg aatggcagct cgatgttgca
     2821 tccaaccgag cggccgccga cttcttttgc gccgcggttg gccgcctcca taatgcccgg
     2881 gccgccgccg gtcatcaccg tgaatccgag ccgggcaatc gccgcgccca tcttcagggc
     2941 ctgttcgtaa tatggatcgt caggtttgat tcgggctgag ccaaacacgg tcacgcaagg
     3001 accaacgaaa tgaagagcgc gatatccgcg caggaaatcc cgcatcactc gcagcaacgt
     3061 gcagaactct gcgaaccgtg agcgcgggcc ttccaggaat atttcgtcgg ctcgctccgg
     3121 cggagcgacc accgctgtca ctgccgaacc cggcgcggaa tcgatcggag cttcacttgc
     3181 cattaacgct taacggccgc tccacagcac ttcaatattt ccgccgcata tgactgggca
     3241 gaatgtttag ctcgctgcgg tatttggcga cggtgcgtcg cgcaatggga atgccgcgct
     3301 cactcaaaat ctcgacgatc tcgtgatcgc tcaacggcgc gctgccgctc tcatgcttca
     3361 cgaggtcaag aatagcctgt ttcacgctgg tgttgctcag cgattcgccg gtcgcagttt
     3421 gatagccgcc tgtgaaaaaa tatttcatct caaaaacgcc ctggggcgtg gacatgtatt
     3481 tgccggacac cgcgcggcta accgtggtct catgcacgcc gacggcttca gcgacttctg
     3541 ccatggtcgt cggtttcaaa tgtgatgggc cgtgctcgag gaaatcgcgc tgtcttgaaa
     3601 cgatttgctg cgcgatgttc atgattgttt gctgccgttg atggatcgag cggatcaaga
     3661 atttgccgct gcggattttg tcccggatgt aatctttgac gtcgctgctc tgcgagtcgc
     3721 cagacgaaat gatgtccttg tacaggttgc tgatccgcag atggggaatc tgttcgttgt
     3781 tgaaggcgat ctgatattcg ccatccacct tttccacgat cacgtccggc aacacatagt
     3841 tctgcggcgc ggcagcgaaa acctggccgg ggcgaggatt caatcgcgcg atgttgtcag
     3901 cggccttttg cacatcctcg acaccgatcg ccattcggcg cgcgatctcc agaaagcgat
     3961 gcctgcccag gtcgtccatg tgctcggaca cgatccggtg ctcgaggctg tgttcttttc
     4021 cctgtcgctg caattggatc aacaaacact cgcgtaaatc acgggcgcca actcccgcag
     4081 gataaaaagc ttggattact ccaagccccc tttcgaaatc gtcctgtgga attccagagt
     4141 tcagtgccat ttcctccggg gtgctttgga gaaatccgtc atcatcgata ttaccgacga
     4201 tcaactcggc agctttccgg tcatcggcgc tgagcacggt ctggtttagt tgcgcgatga
     4261 gattttgctg aagtgtttcc tggactggaa tggaatcgaa caggaactgg cgcttctcgt
     4321 ccgcttcctt cgaaccgcgt cggccgtcga agttggagct ggccgactgc gccatgtaat
     4381 cgcgccattc ctcatccagg ctcgccaatt tttcgaattc gtccttaaat ttctcgtcag
     4441 ctgaggcttc agcttcgcta cgttcattag cgcctgaatc ctccggcaac tcttccagca
     4501 ctggatttgt ttccatctcc tgttgaacca gattgcgcag ctcgagcagc ggagtctgga
     4561 gaatgagcaa gctctgttgc agctgcggcg atagaacctg ctgcaaggcg aggctttgcc
     4621 tctgctgcat tcccaatgcg ggcatgcgcc gagtttaccg gcgtcgtcag cagcaggcaa
     4681 gcaactccgc agtcatcccc atggcgaaac tgtagggcgt ctgtgtcaga cgtcgcagcg
     4741 tttcatacaa cggagccgcg gggacgcgag cgacatagac ggggcaggcc aatggctgag
     4801 tgagcggg
//
LOCUS       DASQUQ010000088         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_925766, whole genome shotgun sequence.
ACCESSION   DASQUQ010000088 DASQUQ010000000
VERSION     DASQUQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925766"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VE486_02430"
     CDS             complement(<1..731)
                     /locus_tag="VE486_02430"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HZA37976.1"
                     /translation="MPRVFPSAVIENLQPLIDGGRHPIKRIVGEDLVVEADIFKDGHD
                     VVAAVLKWRVLGKRPWRETPMTFVDNDRWGAVCTLYDNAIHEYTVEAWADTFRSWQRE
                     FTAKFEGGISALRSEAMEGAALVRSAARRAREPADAARLLEFAEQISTAANSEINAIA
                     QSGELEVLMATYPDRSNAAQYAPAPRVVVERSAALIGAWYEFFPRSAEGRGDRGSTFR
                     QCLPRVDDARAMAFDVIYFPPIHPIG"
     gene            complement(806..1447)
                     /locus_tag="VE486_02435"
     CDS             complement(806..1447)
                     /locus_tag="VE486_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZA37977.1"
                     /translation="MNQRLIAEIGRTQRLQIINSLKRTRGLSVNELVEKMGMSYMGIK
                     QHCLTLQRDGYLDTWRRPQRMGRPEMVYRLTRRSHDLFQADSHQFTLDLLKSVQEIYG
                     PNAPEKLLYNIFEKKTAALKAKAKGETVAERAKWLAHFRDGEGYMSQFTNGDEGGPRI
                     VECHSPIMNLIDRYPIIGRFEQDMFEAVLGTRVRRQMIRNSGLYECAFYFAVK"
     gene            complement(1602..>2276)
                     /locus_tag="VE486_02440"
     CDS             complement(1602..>2276)
                     /locus_tag="VE486_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZA37978.1"
                     /translation="REALPARLERYVIADDVQIEDVSDRFSIFHVLSRTAPSLSDCRI
                     VSVRRLDEPGWDIWTDTARHDDVSQQLSSTFGFVDSATAEIMRIEQGIPRWGCELTDQ
                     IIPIEANLEARAVDYEKGCYIGQEVISRIKMSGQTNKRLCGLISLDDIPLQAGMRLAS
                     TAEKGKQAGWITSATRSEKIGKEIALGFVKRGFTNPGVRMDALAPEESSAVAATSIEV
                     VSLPFR"
BASE COUNT          523 a          655 c          608 g          490 t
ORIGIN      
        1 ccaatgggat gaatcggcgg aaaataaatg acatcgaacg ccatcgccct tgcatcatcg
       61 acccgtggca aacactggcg gaaagtcgag ccgcgatccc ctcgcccttc cgctgagcgc
      121 gggaaaaatt cgtaccatgc gccaatcaac gccgcggaac gctcgacaac aacgcgcggg
      181 gctggagcgt attgtgccgc gttggagcgg tccggataag tcgccatcag cacctcgagt
      241 tcgccggatt gcgcgatggc gttaatctct gaattggcag cggtggagat ttgttcggca
      301 aattccagca accgtgctgc atccgccggt tcccgcgctc gtcgggcggc ggacctgacc
      361 aaagcggcgc cctccattgc ttcgctccgc agcgcagaaa taccgccctc aaattttgcc
      421 gtgaactcgc gctgccaact gcggaaggta tcagcccacg cttccaccgt gtattcgtga
      481 atcgcgttat cgtaaagagt gcagacagca ccccagcggt cgttatcaac gaaggtcatg
      541 ggcgtttccc gccatgggcg cttgccgagg acgcgccatt tcagcacggc cgccaccaca
      601 tcgtgtccat ccttaaaaat atccgcttcg acgacgagat cctcgccaac gatgcgttta
      661 atcggatgac gcccgccgtc gatcaacggc tgcaagttct cgatgaccgc ggaaggaaag
      721 acccgaggca tgggccatca atcaatcgag attcgctgcc tgcgaaagca aaaatctcgg
      781 tagtgccgga gaagaaaact agcagctact tcaccgcgaa ataaaatgca cactcgtata
      841 ggcccgaatt gcgaatcatt tggcgacgga cacgagtgcc gaggacggct tcgaacatgt
      901 cctgctcgaa ccggccaatg atcggataac gatcgattaa attcatgatc ggcgaatgac
      961 actctacgat ccggggtccg ccttcgtccc cgttcgtaaa ctgggacatg tagccctccc
     1021 catcacggaa atgggcgagc catttggcgc gttccgccac tgtttccccc ttggctttgg
     1081 ctttgagtgc cgcagtcttt ttttcgaaga tgttgtaaag gagcttctcc ggcgcgttgg
     1141 gcccgtaaat ttcctggacc gatttcaaca gatcgagcgt gaactgatgg ctatcggcct
     1201 gaaaaagatc atggctgcgg cgtgtaagcc ggtaaaccat ttccgggcga cccattcttt
     1261 gcggccggcg ccaggtatcc aggtagccat cgcgctgaag ggtgaggcaa tgctgcttga
     1321 tgcccatata gctcatgccc attttttcca ccagctcgtt aacagacaac ccacgagtgc
     1381 gcttgagaga gttaatgatt tggagccgct gggtgcggcc aatttctgcg attaaacgtt
     1441 gattcataaa gtgacggttg ggtgacaaac acgcgctgaa aaagcgctga aagactgact
     1501 ttacacgccg tgatctttaa aaagcaagtt gaaagtgcac ccaactgccg aaaacaaaac
     1561 aaattacaga aagcagcgcg gctacgcgcg tgtgtaacaa tttagcgaaa cggaagcgaa
     1621 acgacttcga ttgacgttgc cgccaccgcg ctggattctt ccggtgccag cgcatccatc
     1681 ctgacgccgg gattagtgaa tccgcgtttc acaaaaccga gtgcgatttc ctttccaatt
     1741 ttctcgctgc gcgtcgcgct cgtgatccag cccgcttgtt tacccttctc tgccgtggag
     1801 gcgagtctca tgccagcctg aagcgggata tcatccaacg aaattagccc acatagtcgc
     1861 ttattggtct gacccgacat tttaatgcgt gagatcactt cctggccaat gtagcaacct
     1921 ttttcataat cgacggcgcg cgcttccaga ttcgcctcga ttggaataat ctggtctgtc
     1981 agctcgcagc cccaacgggg gatcccctgc tcgattcgca tgatctccgc ggtggcagaa
     2041 tcgacgaagc cgaatgttga ggacaactgt tgtgagacat catcatgcct cgctgtatcg
     2101 gtccagatat cccaccccgg ctcgtcgagg cggcgcacgg aaacaattct gcagtcgctt
     2161 aagctcggcg cagtgcgcga caggacgtga aagatcgaga atcggtcgga gacgtcttca
     2221 atctgaacgt cgtcggcgat cacataccgc tccagtctgg ccgggagtgc ttcgcg
//
LOCUS       DASQUQ010000089         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_835802, whole genome shotgun sequence.
ACCESSION   DASQUQ010000089 DASQUQ010000000
VERSION     DASQUQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835802"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VE486_02445"
     CDS             complement(<1..1268)
                     /locus_tag="VE486_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZA37979.1"
                     /translation="MATNGIHPLEALMRERIVVLDGAMGTMIQQCKLSESDYRGERLR
                     DWQGNDLKGSLELLNLTQPQVIAKIHSDYLKAGADIIETNTFSGTTIGLHDFLFQGEP
                     GSGRKDQEFFQRVIDDARLRALVYEINSEAARIARHAADHATNETGQRRFVGGSIGPL
                     PVAGSISPDVNDPAFRAVSFDQLRQTYFDQAKALLKGDVDLLIVETIFDTLNGKAALF
                     AITDAFAETGRTVPLMISGTVIDKSGRNLSGQTVEAFLVSIAHAQPLILGLNCSLGPD
                     EMEPFIEELSRIAPFYVGAYPNAGLPDPLSPTGFPETAESFAPKLAAWVANGWLNIVG
                     GCCGTTPEHIRKIAEAVRGVPPRRAEPRNSGSARASRAGFGAFAETSTNALYGKRRLP
                     HFERPWGKYAVTFSTRNHRQLAPHERDIVF"
     gene            complement(1726..2484)
                     /locus_tag="VE486_02450"
     CDS             complement(1726..2484)
                     /locus_tag="VE486_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37980.1"
                     /translation="MKSTITIIAIALFGFLPVTFVHGQTSPKPAAGAKQAATAGAGKR
                     TDVYHVHFAKAALGKALQLGDWLKTPDPTNPMPDHFIVLRHQDGDAWDYVVITHLGPK
                     ATVEAAGTAVPPDKRDLGAWHTDTFVNGPSWEEFTKAMGIDADSKSKSAGSVYSVSYY
                     RPAPGHREQLEKILGELPSAANDTTAGNVLMQHLEGADWTFLAIARYNSWDDFATGEK
                     NSVGQQTSKKDSPWARMRDHSDFHTDTLTDRIAP"
BASE COUNT          605 a          735 c          791 g          640 t
ORIGIN      
        1 tgaaaaacaa tgtcgcgttc gtggggggcg agttggcgat gatttcgggt cgaaaatgtt
       61 accgcgtatt taccccaagg gcgctcaaag tggggcagcc tacgtttgcc gtacaaagcg
      121 ttcgttgaag tttcggcgaa ggcgccgaaa ccagcacgcg aggcgcgtgc gctccccgaa
      181 ttgcgcggtt ccgcgcgccg aggtggaact ccgcgcacgg cctccgcgat tttgcggatg
      241 tgctccgggg tagtcccgca gcaaccgccc acaatattca gccaaccgtt tgccacccaa
      301 gcggcaagtt tgggtgcaaa cgattctgca gtttccggaa atccggtcgg cgaaagcgga
      361 tcgggcaatc ctgcatttgg gtatgcgccg acgtaaaacg gcgcgatccg tgaaagttct
      421 tcgatgaatg gttccatctc atccggcccg agcgaacagt tcaaacccag aataagaggc
      481 tgcgcgtgcg cgattgaaac aaggaacgcc tccacggttt ggccgctcaa gttccttccc
      541 gatttgtcga tcaccgtgcc cgagatcatc aacggcacag tgcggccagt ttctgcaaat
      601 gcatcggtaa tggcgaacaa cgcagccttg ccgttgagcg tatcgaaaat ggtttcgacg
      661 atgagaagat cgacatcacc tttgagcagc gccttcgctt gatcgaaata agtttggcgt
      721 agctgatcga acgagaccgc gcgaaatgcc ggatcgttta cgtcaggtga aatggagccc
      781 gcgactggaa gcggtcctat cgatccacca acgaatcgtc gctggccagt ctcattcgta
      841 gcgtggtccg cggcgtgacg cgcgatccgc gctgcttcgg aattaatttc ataaactagc
      901 gcgcgaagcc gtgcatcgtc gatcacgcgc tgaaagaatt cctgatcttt gcggccactt
      961 ccaggttcgc cttggaaaag aaaatcgtgc aaaccgatgg ttgttccgct gaaggtgttc
     1021 gtttcgatga tgtccgcgcc ggctttgagg taatcggaat gaattttcgc aatcacctgc
     1081 ggctgcgtca ggtttaatag ctcgaggctg cctttgagat cattgccttg ccaatcgcgg
     1141 agccgctcgc cgcggtagtc cgattctgac aatttacatt gctggatcat agttcccatc
     1201 gcgccatcga gcacgacgat ccgctcgcgc attaacgctt ccagaggatg aatcccgttt
     1261 gtggccataa tgtccggcaa ctaaacggca tttcgtgggg ggatcaagaa acaaatctac
     1321 gcatcatgat gcgtagatgc gttaaacccc tggtcgtgct cctaatcgta atccaaaaac
     1381 gatcgcaata aataaccgct gccgcgttaa caatgccgga cgccgaatgc cgaaggaacg
     1441 tcaaatgccc gacattttag gtagggggcg tcactcttgt gccgaggctt ggcgggcaga
     1501 ggaccgccag ccctacctgc gccacgtgca ccccgtgtcg ttgtaaaggc ggttgtgtca
     1561 accgccttgg gcaggcgaat tttgtagggc gcctgtatca gacgccaatc ttcatttggg
     1621 tcggcgtttc acagaatgga atcgcggggt tgcgagcgac atagacgggc aggccgggag
     1681 gccgagtgag cgggagcgaa tgagtcaaac gccctacaaa aactcttatg gggctatgcg
     1741 atccgtgagc gtgtcggtgt gaaaatcgga gtgatcacgc atacgagccc aagggctgtc
     1801 ctttttgctt gtctgctggc cgacgctgtt tttctcgcct gtggcaaaat cgtcccacga
     1861 attgtagcga gcgatcgcca gaaacgtcca atcggcacct tcgagatgct gcattaatac
     1921 atttccggcg gtggtgtcgt ttgccgcgct tggcaattcg ccgagtattt tttccagttg
     1981 ttcgcggtga ccgggagcag ggcgatagta agagacagaa taaaccgatc ccgcactctt
     2041 ggatttcgaa tcggcgtcga tacccatggc tttcgtgaac tcttcccacg aaggaccgtt
     2101 cacaaacgta tcagtgtgcc acgcacctaa atcgcgtttg tccggaggca cagccgttcc
     2161 cgcagcttca acggtcgctt ttgggccgag atgcgtgatc accacataat cccatgcgtc
     2221 gccgtcttga tggcgaagaa caatgaaatg atcaggcatc gggttagtgg gatcaggcgt
     2281 ctttagccaa tcgcctagtt gcagggcttt gcccagggcg gcctttgcga aatggacatg
     2341 atagacgtcg gttcgtttgc cggctccggc agtcgccgcc tgctttgcgc ctgcggcagg
     2401 ttttggtgac gtctgtccgt gaacaaatgt aaccggtaag aagccaaaca atgcgatggc
     2461 gatgatggta atggttgatt tcatgatggt gttgtctctt tctgtttttg gttaaaccgg
     2521 agcccttggg gatgcgcgcc gtcagcggag cgagcgacgt taaagcgtcc ttcgctgcgt
     2581 tgtttcgtta ggtatgctgg ccgacggctt cggtgtcaat gcttatttcg agctccggaa
     2641 acgaccgact attcgcgacg actacgaatc gattattagc ttttttcagc ttggcggaac
     2701 aaaaagcacg caatcaccgc tcaatattcg gcaacggtct cgtcgggata gcagtacaac
     2761 caatgctcgc c
//
LOCUS       DASQUQ010000090         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_558383, whole genome shotgun sequence.
ACCESSION   DASQUQ010000090 DASQUQ010000000
VERSION     DASQUQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558383"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1637
                     /locus_tag="VE486_02455"
     CDS             276..1637
                     /locus_tag="VE486_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZA37981.1"
                     /translation="MHQTLVTPPTAAATAKKRRSRRRRYIIFGSIGLLVLWIVVSIIL
                     GKREKPIPVTTEKALRKTILQTVSATGKIQPEIEVKISPEVAGEIIELPVEDGMRVKK
                     GDLLVKIKPDSYKALLEQQEAAISTAKATNLQQKATMMKTEQDFKRAEDLFNQKLISA
                     QEYNAAEAAYDVAKNTYESSLHEIERAQAGSSQARDQLSKTTVYSPIDGTVTILNSKL
                     GERIVATGQFAGTEVMRVADLGHMEARVDVNENDVVNVKVGDNANVKIDAYGDRKFRG
                     TVYQIANTGKTTGAGTQEEVTNFEVKIRIDDHDVTLKPGLSCTADIETDMVKDVVAVP
                     MQSVTIRTGDSNLSPEEIEKQKQKAAARDKGDNNAEFVNERLERQAQKEGREKLAKVV
                     FLKNGNKAQMVKVTTGISDDTYMEIKSGIKPDDEVISGSYGAISRKLKDGAKVAIDKE
                     ATK"
     gene            1680..2468
                     /locus_tag="VE486_02460"
     CDS             1680..2468
                     /locus_tag="VE486_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA37982.1"
                     /translation="MKPTLQHPNTPTLGMTNGDTTRSPGPIMIDIDNITKDYVMGEEV
                     VHALRGVSLRIHRNEYIAIMGPSGSGKSTLMNMLGCLDTPTSGQYEFNGRNVSAMDDD
                     ELAAIRNREIGFVFQTFNLLPRANSLRNVELPLIYAGIDPETREERAAQVLQDVGLGD
                     RLHHKPNELSGGQRQRVAIARALVNDPSIILADEPTGNLDSKTGEEIMTLFEDLYQRG
                     HTIILVTHEADIARHARRTVRLRDGLIESDDAMVAPEFEGAVAS"
     gene            2651..3901
                     /locus_tag="VE486_02465"
     CDS             2651..3901
                     /locus_tag="VE486_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA37983.1"
                     /translation="MKRFLYELSESWKIAAAQMRANKTRSTLTALGVIIGIVAVTLMG
                     TAISGIQIGFDRSMAVLGEDVLYVGQWPWKPVDDWWNYRDRKKIKTEYAETLNRMIAA
                     TPNSNLLVAVPTSSLLRSIKYGGSEVQNVFVLGTTSDFIVTSTVDCKEGRFFNELESR
                     SGASVCIIGYDVADALFPSESPIDKVVLINGQNFKVVGVNARQGTFLGLFSWDSMVAM
                     PLGAFNKYFSTKSESDIRVKVKDKTKMADAKDELTGLMRRVRGLPPEKKDDFSINEQQ
                     AFKSTLDPVKNSIAIAGLFITGLSLFVGAIGIMNITFVSVKERTKEIGTRKALGARRR
                     TILLQFLIESTALCLLGGLIGLTFAYVMCFGIGKAFPSFPIHFSFGLVFASMIVSILT
                     GLFSGFAPAWSASRLDPVTALRYE"
     gene            4022..>4684
                     /locus_tag="VE486_02470"
     CDS             4022..>4684
                     /locus_tag="VE486_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA37984.1"
                     /translation="MSSLGTNKLRAALTMLGITIGVFSVISVMTAVGALQNSIEGGLS
                     FLGSNIFQFAKYPANVSAGGDVKEKYQNRRNISYRQALRYYELMEGYASEICLKTFAY
                     EVKGQAVYNGVKTTPNLLIVGTNRGFLAANAHTVEYGRNLNDEDVAFSRRVVVIGKII
                     EKRLFPQETPIGKVIRVSGHTYTVIGVLAEKGSAFGQSRDDLCLMPITRFFEDYGEVN
                     RTV"
BASE COUNT         1178 a         1200 c         1338 g          968 t
ORIGIN      
        1 tgcggaacgt ttgcgtggcg ttgggtaacg taggcgatcc gaacgatttg ccagcgctcg
       61 aacgagcggc gtcggatccc gagtcgttga tcgcggagca tgctgcgtgg gccatcgagc
      121 gaatcgggaa acgcttcgcg attgagacga ccaaaattaa ttgaatccaa aggcaacacg
      181 cacgtatcca aattatgtcc agtgaacgcg acttgctttg caaaagtcta aagtgccatt
      241 atttgtgacg cccccgtccg tgtaagtccg atcccatgca tcaaacccta gtgacacctc
      301 ccactgcagc cgcaacagcc aagaagcgcc gctcccggcg caggcgctac atcattttcg
      361 gttccatcgg tctcttggtc ctgtggattg tcgtttccat catcctgggc aaacgcgaaa
      421 aaccgatccc ggtaacaacg gagaaagcct tgcgcaagac tattctgcaa actgtctccg
      481 cgacgggaaa gatccagccc gaaatcgaag tcaaaatttc gccggaagta gctggtgaaa
      541 ttattgaatt gccggtcgag gacggaatgc gcgttaaaaa gggcgatctg ctcgtgaaaa
      601 tcaaacccga ttcctacaaa gctctgctcg agcagcagga agcggcgatc agcacggcga
      661 aggcaacgaa tttgcagcag aaagcgacga tgatgaagac cgagcaggac ttcaaacgag
      721 ccgaagattt atttaaccag aagctgattt cagcccagga atacaacgcc gcagaagctg
      781 cttacgacgt ggccaagaac acctacgaga gttcgctgca cgagattgag cgcgcgcaag
      841 cgggctcgag tcaggcacgc gatcaacttt caaagacaac cgtttattcc ccaatcgatg
      901 ggacggtgac aattttgaac agcaaactgg gcgagcgcat cgtagcgacg gggcaatttg
      961 cgggaacgga agtgatgcgc gtggccgatc tcggccatat ggaagcgcgg gtggatgtga
     1021 acgagaacga cgtcgtaaac gtaaaggttg gcgacaacgc taacgtgaag atcgacgcct
     1081 acggtgaccg gaaatttcgc ggcaccgttt accagattgc gaacacagga aagaccaccg
     1141 gcgcaggaac gcaggaggaa gtaaccaact ttgaggtaaa aattcgtatc gacgatcacg
     1201 atgtgacatt gaaaccaggg ctaagttgca cggccgatat cgaaacggac atggtgaagg
     1261 acgtcgtcgc ggtgccgatg cagagtgtga cgatccgtac cggcgacagc aatttgagcc
     1321 ccgaagagat tgagaagcag aaacaaaagg ccgcggcgcg tgacaagggc gacaacaacg
     1381 cggaattcgt caacgagcgc ctggagcgac aggcgcagaa agaagggcgt gagaagcttg
     1441 ccaaagtcgt tttcctcaaa aacggcaaca aggcgcagat ggtcaaagtg acgacgggga
     1501 tttccgatga cacttacatg gaaatcaaga gcgggattaa gccggacgat gaagtgatct
     1561 ccggtagtta cggcgcaatc agccgaaagc taaaggacgg tgccaaggtt gccatcgaca
     1621 aggaagcaac gaaatagcgg agtgatggag tagtggacta atggagtcat gaattcactt
     1681 tgaaaccaac cctccagcac cccaatactc caacactcgg catgaccaac ggtgatacaa
     1741 cgcgatcgcc cggcccgatc atgatcgaca ttgacaacat cacgaaagac tacgtgatgg
     1801 gcgaagaggt tgtgcacgct ttgcgcggcg tatccctgcg tattcatcgc aacgaataca
     1861 tcgccatcat ggggccgagc ggaagcggga aatcgactct tatgaacatg ctcggctgcc
     1921 tcgatacgcc cacttccggc caatacgaat tcaatggtcg gaacgtttcg gccatggacg
     1981 acgacgaact ggccgccatt cgtaatcgcg agattggatt cgtctttcag acattcaatc
     2041 tgcttccacg cgcgaacagt ttgcggaatg tcgagctgcc gctcatctac gccgggatcg
     2101 acccggagac gcgcgaagag cgcgccgcgc aggtcttgca ggacgtcgga ctcggcgacc
     2161 gtctgcatca caaaccgaat gaactctccg gcgggcaacg ccagcgtgtg gcgattgcgc
     2221 gcgccctcgt gaatgatccc tccattattt tggccgatga gccgaccggc aacctcgatt
     2281 ccaaaaccgg tgaagagatc atgacgctct tcgaggatct ttatcaacgc ggccatacaa
     2341 ttattctcgt cacacacgaa gctgatatcg cccgccacgc ccggcgaacg gtgcgtctgc
     2401 gtgatggttt gatcgaaagc gacgacgcaa tggtggcgcc cgaattcgaa ggggctgtgg
     2461 cgagctaaaa tcgaggaatg cggatgagct cgcgagacgc aactcttgct ctgggtagca
     2521 caggttgcca gcctgctcca ttcggcagta ctgccgaatg catctttttg ccaaaggcgg
     2581 gaccaaatct cgcatctgtt cgcggcaagc tgccgcgaac tacaggctgg cagcctgtgc
     2641 tccccaaaac atgaaacgct ttctctacga gctcagcgaa agctggaaga tcgccgcggc
     2701 gcaaatgcgc gccaacaaaa cccgctccac gctcaccgcg cttggggtca tcattgggat
     2761 tgtcgcggtg acgctaatgg ggacggcgat cagcggaatt caaatcggtt tcgacaggag
     2821 catggctgtg ctgggcgaag acgtgctcta tgtggggcag tggccgtgga agccggtgga
     2881 cgattggtgg aattatcgcg atcgaaaaaa gatcaaaacc gaatacgcgg aaacacttaa
     2941 ccgcatgatc gcggcaacgc ctaattcgaa tctgctcgtg gcggtgccga cgtcgagttt
     3001 gcttcgttcg atcaaatacg gaggcagcga agtccaaaat gtgttcgttc ttggaacgac
     3061 ttcagatttc atcgtaactt cgaccgtcga ttgcaaagaa gggcgtttct ttaacgaatt
     3121 agaatcacgc agcggcgcca gcgtttgcat catcggttac gatgtggccg atgcgttgtt
     3181 cccgtcggaa agccctatcg acaaagtcgt cctgatcaac ggacagaatt tcaaggtcgt
     3241 gggcgtgaac gcgcgccagg gcacttttct cggtttgttc agctgggatt cgatggtggc
     3301 gatgccgctc ggcgccttca ataaatactt cagcacgaaa agcgaatcgg acattcgggt
     3361 gaaggtgaaa gacaaaacga aaatggccga tgccaaagac gaactgaccg gtctgatgcg
     3421 gcgcgttcgc ggtttgccgc cggagaagaa agacgatttc tccattaacg agcagcaggc
     3481 gtttaagagc acgctcgatc cggtaaaaaa ctcgatcgca atcgccgggc ttttcatcac
     3541 agggctgtcg cttttcgtcg gggcgatcgg gattatgaac atcacgtttg tcagcgtgaa
     3601 agagcgaacg aaggaaatcg gaacccgcaa agcgcttggg gcgcggagac ggaccatcct
     3661 tcttcaattt ctcatcgaat ccactgcgct ctgtctgctg ggcggactca tcggattaac
     3721 gtttgcttat gtgatgtgtt tcggaatcgg gaaagcgttc ccgtcgtttc cgattcattt
     3781 ttccttcggg ctggttttcg cgagcatgat cgtgtcgatt ttgaccggtc tcttctcagg
     3841 atttgctcca gcctggtccg cgtcgcgcct cgatccggtg acggcgctgc gctatgagta
     3901 ggggtaatgg agtaatggaa cgctggagta atgaattggt tccggcgtca acgttccatt
     3961 actcacctac tccattactc cagttctgag tatgttgttt cgtgagatca tagcaatggc
     4021 gttgagttcg ttggggacga acaagttgcg cgctgcgctg acgatgttgg gcattaccat
     4081 cggcgttttc tcggtcatct cggtgatgac ggcagtcggc gcgctccaga attctattga
     4141 gggcggcctg agttttctcg ggtcgaacat tttccaattc gccaaatacc cggcgaacgt
     4201 gagcgccggg ggcgatgtga aagaaaaata ccaaaaccgg cgcaacatca gttaccggca
     4261 agcgttgcgt tactacgaac ttatggaggg atatgcatcc gagatttgtc tcaagacttt
     4321 cgcttatgag gtaaaagggc aggcggttta caacggcgta aaaacgacgc caaatctcct
     4381 gatcgttggg acgaatcgag gttttcttgc agcgaatgct cacacagttg aatacggccg
     4441 gaatctgaac gatgaagatg tcgccttttc ccgccgcgtg gtcgtaatcg gaaagataat
     4501 tgagaagcga ttatttccgc aggagacgcc catcggcaaa gtcatccggg taagcggcca
     4561 cacgtacaca gtgatcggtg ttctggcgga gaaaggcagc gcgttcggtc aaagccggga
     4621 cgacctttgt ctcatgccga ttacgcgatt ctttgaggat tacggcgaag taaaccgcac
     4681 cgtc
//
LOCUS       DASQUQ010000091         4670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_836433, whole genome shotgun sequence.
ACCESSION   DASQUQ010000091 DASQUQ010000000
VERSION     DASQUQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836433"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1260
                     /gene="eno"
                     /locus_tag="VE486_02475"
     CDS             1..1260
                     /gene="eno"
                     /locus_tag="VE486_02475"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZA37985.1"
                     /translation="MIGREILDSRGNPTIEVDVRLESGALGRAAIPSGASTGEHEAWE
                     LRDGDKARFSGKGVAKAVANVNDKIAPALKGWDARDQRKIDHKLIELDGTSNKRNLGA
                     NALLGVSLAVAHAAAAAENLFLFQYLGGLKARVLPVPMMNILNGGAHSDAPIDFQEFM
                     IVPRGAPTFSEALRYGVEIFHALKSVLKDRHLSTAIGDEGGFAPQLDSAEDALEAISS
                     SVKKAGYKLGEQIFIALDPAASEFYDSENNVYVFKKSDGSKRTSDELIDYYAGLCARF
                     PIISIEDGCAENDWEGWKKLTAKLGEKIQLVGDDLFVTNVAFLRKGIAEQVANSILIK
                     VNQIGTLTETLATIDLAKENNYSTVISHRSGETEDATIADIAVATNAGQIKTGSLSRS
                     DRIAKYNQLLRIEEELGDKAEYGGKIR"
     gene            1261..1626
                     /locus_tag="VE486_02480"
     CDS             1261..1626
                     /locus_tag="VE486_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZA37986.1"
                     /translation="MDQTYADFRARREATVWQRLNRILRVLLVLAIWLVIVSLFVPPY
                     KKLMQSRAEIDELQQQVNGQQSLLSRQTREVNLLKTDVTYLETIARDRLDLMKEGETI
                     FRLEPAPTAKPKPIDSARR"
     gene            1916..2992
                     /gene="prfB"
                     /locus_tag="VE486_02485"
                     /pseudo
     CDS             1916..2992
                     /gene="prfB"
                     /locus_tag="VE486_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            3119..3418
                     /locus_tag="VE486_02490"
     CDS             3119..3418
                     /locus_tag="VE486_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37987.1"
                     /translation="MRATAGQTCHCAFDPTHARKIAPATWTPVKITPSTFADWEWEIM
                     TALLVANSEIAASLQPFNTMRQNPAPAGTKLREDVGQLMSQSSLDFRGMMNELWI"
     gene            complement(3678..>4670)
                     /locus_tag="VE486_02495"
     CDS             complement(3678..>4670)
                     /locus_tag="VE486_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZA37988.1"
                     /translation="MLRVKPHEIIFTSGGTESCNLAVLGLARCLNSSGGHVISNKGEH
                     HSVLNALEYLEKRENFEVTWLNVSEQGIVDLDQLAESIRPETRLVSIMAVNNETGVIQ
                     PIHEISQICRERGVLLHSDMVQAFGKIETDLSLVDASSFASHKFYGPKGAGFLFLRSG
                     LSIQPIMFGGAHENERRPGTENVAAIAGMAAAAEWTLRDRQIEQKREAQLRDELWTHI
                     AGAFPAAKQNGDSADRLANTLNVSFTGFDSETMLMALDLEGVCASSGSACMVGSVVAS
                     HVLLAMGLPVERARSAVRFSLGKWTTAEEISGAGEAIERIARRLNKADSAYAVA"
BASE COUNT         1114 a         1285 c         1311 g          960 t
ORIGIN      
        1 attattgggc gcgaaatcct ggattcgcgc ggcaatccaa ctatcgaagt cgatgtgcga
       61 ttggaaagcg gcgcgcttgg ccgggcggcg attccaagcg gagcaagcac cggcgagcat
      121 gaagcgtggg aattgcgcga tggcgacaag gcccggttca gcggcaaagg cgttgcgaaa
      181 gctgtcgcca atgttaacga caagattgcg ccggctctca aaggctggga cgcgcgcgac
      241 caacgaaaga tcgaccataa gctcatcgag ctcgacggca cgtcgaacaa aaggaacctc
      301 ggagcaaacg cgctactcgg cgtttccctg gcggtcgcgc atgccgctgc tgcggcggaa
      361 aacctttttc tctttcaata tcttggcgga ctcaaggcta gagtgttgcc cgtgccgatg
      421 atgaacatcc tgaacggcgg cgcgcactcg gacgcaccga tcgattttca ggaattcatg
      481 atcgtgccgc gtggtgcgcc tacgttttcc gaagcgctgc ggtacggcgt ggaaatcttc
      541 cacgcgctga agtccgtgtt gaaagaccgg catctttcca ctgcgattgg cgatgaaggc
      601 ggctttgcgc cgcagctcga ctccgccgag gacgccttgg aagcgatttc gagtagcgtt
      661 aaaaaagccg gctacaagct cggcgaacaa atctttatcg cgctcgatcc agctgcctct
      721 gaattctacg acagcgagaa taacgtttac gtcttcaaga aatcggacgg ctcaaaaaga
      781 acttccgacg aactaattga ttactatgcc ggtctttgcg cccgttttcc gatcatttcg
      841 atcgaggacg ggtgcgcgga aaatgattgg gagggctgga aaaaactgac cgcaaagctt
      901 ggtgaaaaaa ttcaactggt cggtgacgat ctctttgtca ccaacgtcgc atttctgcgc
      961 aaaggaatcg ccgagcaggt ggcgaattcg attttgatca aggtgaatca gatcggcacg
     1021 cttaccgaaa cgctggcaac gattgatctg gcaaaggaaa acaattactc gaccgtgatc
     1081 agccatcgct ctggcgaaac ggaggacgca acgattgccg atatcgcggt cgcgacgaac
     1141 gccggccaaa tcaagaccgg gtcacttagc cgcagcgacc gcatcgcgaa atacaatcag
     1201 ctcctgcgaa tcgaagaaga acttggtgac aaagccgagt acggcggtaa aatccgctaa
     1261 gtggatcaga cctacgccga ttttcgcgcg cgtcgcgaag ccaccgtctg gcagcgactt
     1321 aaccgaattc ttcgcgttct gctggtcctc gcgatctggc tcgtgatcgt gagcttgttt
     1381 gttccaccct acaagaaatt gatgcagagc cgcgccgaaa tcgacgagct ccagcaacag
     1441 gtaaacgggc aacaaagttt gctctcgcgt cagactcgcg aggtcaacct cctcaagact
     1501 gacgtgactt atctggaaac catcgcgcgc gatcgtctcg atttgatgaa ggaaggcgaa
     1561 acaatttttc gcctggaacc ggcgccaact gcaaaaccaa aacccatcga ttccgcgcgg
     1621 cggtagcgta cgcacttctg gggagcacag gctgctagcc tgtcgttttc ggtagcctgc
     1681 cgaaaagctc tttgaacatt cgataagaga gattcttgca acgcgtcgga gtcgtcggct
     1741 agctgccgac gacaacaggc tggcagcctg tgctcctcgc agatcagaga tgcgcaggcg
     1801 gagaatgccc ccgctttgca tgcaacgccg cttgaattat cttcctccat gccaacggaa
     1861 atctccatcc ctgatgccga agatttgaaa tcgcgcgtcc gcgaactgcg gaggtttctt
     1921 tgacgtcgaa aagctccaaa caaatctagt aaccatcgag gggcgcatgg ctgcgcccga
     1981 tttctggtcg aatcgtgagc gtgctcaggc cgatatcgag gaggtttccc gattacgctc
     2041 gctggttaat ccgtttcaag agctggaacg cgagattgcg gattttagcg cgctgcagca
     2101 gctggctgcg gaagagaccg attccactgc tcgcgctcac gcagagaaag aagtcgcgac
     2161 cgaacatgac cgcttaattc acaagttgga agaatttgag ctgcgccaat ttctttccag
     2221 cgaaaacgat cgcgccaatg cgtttctcac cattcactcc ggcgcgggcg gtactgagtc
     2281 gtgtgattgg gccgacatgt tgctgcggat gtatcagcgc tggattgagc gaagcggctt
     2341 caaatcacag accgtggaca tccaacaggg cgaagaagtt ggcatcaaat cggtgaccct
     2401 gctcgtgagt ggcgattatg cctacggtca tcttcagacc gagcgaggcg tccaccggct
     2461 cgtgcgtatt tcacccttcg atgcaaacaa acggcgccac acatcgttcg ctagtgtgga
     2521 tgttgtccct gaaatcaccg agactgctcc gatcgaaatt aaccctgccg acctggagat
     2581 cgacacattc cgaagcggcg gaaaaggcgg ccagaacgtc aacaaagtcg aaaccgctgt
     2641 gcgcattctg cataagccga gcggcatcgt ggtcgcctgc caggctgaac gcagccaggg
     2701 acgcaatcgt gaactggcga tgaaaatgct gaaagcgaag ttgtacgaaa tcgaacagga
     2761 caaaaagcgc gccgagatcg atcggcaata tagcgagaag ggcgagatcg cgtggggaaa
     2821 tcaaattcgc tcctatgttt ttcagcccta ccagatggtg aaagaccacc gcactggagc
     2881 ggaaacgagc aacgtgcagt atgtcatgga tggcaatatc gacatgttca tccaggcaaa
     2941 gctgaggggg gagaaagcgg gtaagagcga ggaagagggc gccgaaattt agatagggac
     3001 ggatttccga gccgtccgac ttattcaggg attcgacgga cgtcccggag gtgcgtccct
     3061 acctaactag ggttgagacg aggcgcagcc ggaaccgcgc atcgaatttt ccgctgctat
     3121 gcgcgcgaca gcaggccaga catgccactg cgcgtttgat ccgacgcacg cgcggaaaat
     3181 cgcgccggca acctggacac ctgtaaagat aacgccgagc acgttcgctg attgggaatg
     3241 ggagattatg acagcgcttc tcgtcgctaa ttccgagatc gcggcaagcc tgcagccatt
     3301 caataccatg cggcaaaatc cggcgccggc cggcacgaaa ttgcgcgagg acgtgggcca
     3361 gctcatgtcg caaagttcgc tcgatttccg agggatgatg aacgagttgt ggatttaacg
     3421 agattaatct ggatcgacaa tcggcccggc cagcacaagt tttgaggcgt gcgttccact
     3481 cgacgcgaag ctcgatcgcg attcgatctg cacccagggc gcgcagtagg tggcgagcca
     3541 agcgcagtag cccggccgtc tcggctgtga ggccggcggg catcctgcct gccggttcaa
     3601 ttatggattc gccaagcgag acgcccggct tccccacagg caggattttt gtgccaggaa
     3661 acggaaattc gagttgccta agcaacggca tatgcgctat cggctttgtt caatcgtctt
     3721 gcaatccgct cgattgcttc cccagcacca ctgatttctt ccgcagtcgt ccatttgccc
     3781 agcgaaaagc gaacagccga gcgggctcgt tccacaggca aacccatcgc gagcaaaaca
     3841 tgagacgcaa cgacagatcc gaccatgcaa gctgatccgc tcgaggcgca gacgccttcc
     3901 aagtcgagcg ccatcagcat ggtctccgaa tcgaaaccgg taaaactcac attgagcgtg
     3961 ttagcgaggc gatctgctga atcgccgttt tgcttcgctg caggaaaagc gccggcgatg
     4021 tgcgtccata actcgtctcg caattgtgct tcgcgctttt gctcaatctg gcgatcgcgc
     4081 aatgtccact ccgcggctgc tgccattccg gcgattgcag cgacgttttc agtgcctggt
     4141 cgccgctcat tttcgtgggc gccgccgaac atgattggct ggatcgacaa tccgctgcga
     4201 agaaagagaa atcctgcgcc ttttggccca taaaatttgt gcgaggcaaa actggaggca
     4261 tcgaccaggg agaggtcggt ctcgatcttt ccaaacgcct gcaccatgtc gctgtgtaac
     4321 aggacaccgc gttcccggca gatttgcgaa atttcgtgga ttggctgaat cacgcctgtt
     4381 tcgttattga cggccatgat cgacacgagt ctcgtctcgg ggcgaatcga ctctgcgagt
     4441 tgatcaagat cgacaattcc ttgttccgaa acattgagcc acgtcacttc gaaattctcg
     4501 cgtttctcca aatattcgag cgcattcaga accgaatgat gctcgccctt atttgagatc
     4561 acgtgaccac cgctcgaatt caggcagcgg gccagcccca gaacagcgag attacacgat
     4621 tccgttccac cgcttgtgaa aatgatttca tgcggtttga cccgcagcat
//
LOCUS       DASQUQ010000092         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_558733, whole genome shotgun sequence.
ACCESSION   DASQUQ010000092 DASQUQ010000000
VERSION     DASQUQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558733"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..2829
                     /locus_tag="VE486_02500"
     CDS             187..2829
                     /locus_tag="VE486_02500"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZA37989.1"
                     /translation="MYRLPVAKFFSAVLLFTMVNLKGAPSEPSSTITFPPSSAQKWVL
                     PNGLTIIVQEDRTAPVASMQAWCATGSIDEDAHLGAGLSHILEHMLFKGTKTRSTNEI
                     AQKIQDVGGYINAYTSFDRTVFWIDVPKDGVAVGLDVLADAMMNSTLPPEEYQKEQEV
                     IRREFAMGMDDPDRMAGLLLFATAYQRHPYRFPVIGEIEIYNQLTQEQVMQYYKTRYV
                     PNNLTFIVVGDVDGEKVRQQLTDLFKPYPEKSLKPVFIPAEPPQLGRRELNKEFPTEL
                     THLSLAWHIPEVTNPDVPALDLLSTILGDGRSSRLYRRVREEAGLAYGIAAFSYTPGD
                     PGLFGIDATLDPKKRQAAEQLVLRIINEVKQTGVTSEELAKAKKMSLSHHLEALTTMR
                     GQASDIGSNWLLTRNLNFSRDYLDAVQKVSLDDIKRVAANYLTDSNLTVISLNPKGSL
                     AAKPEGTKAAAAGEIQKFELSNGLRILVREDRRLPLVAMGAVFRSGLLAETAQTNGIT
                     RLMAKVLLKGTKTRTAEQIADEIERVGGSIGSDAGNNSFSVSLDVMKPDLKLGVELFA
                     DVLLNATMPEKAIAREKEVQIAAIQQEEEQLTAVARNILRQALFPQHPYALRANGLVE
                     SVQRLNQKDLLEFRDRYLVAKNGVVFVFGDVKAAEVKQLFEQALGGMKPGALALTDAH
                     PAAPLSKIETVESRKDKAQGVIMVGYRGADLFSPDRYALELIDEASSDLGSRFFVRIR
                     EQMGLAYYVGASQMQGLVPGLFAFYLGTDPQKIEPVKTALLDEIHKLASDGLTSEELA
                     RAKKKLIGQQEIANQSNAAFGYHCALDELYGLGFNYYKQLEHDVNAVTLDEIKRVAAK
                     YFRDQPYVLATVRPPEGAAAAKQK"
     gene            2833..3273
                     /locus_tag="VE486_02505"
     CDS             2833..3273
                     /locus_tag="VE486_02505"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HZA37990.1"
                     /translation="MAEVQKTTLSGELSQRFIEFVMMHAQNAALFLGQIPNPKTGEGE
                     VNLELAKMFIDQLAMIQEKTRGNLTSEESTVLRNALSNLQMVYVEVAREAPKGAAQPE
                     QPAPSPKPAEQTSPSTPTPEPSAPISSTESETESKKKFTKSYGP"
BASE COUNT          899 a          986 c         1030 g          787 t
ORIGIN      
        1 tcggctggct cgagccagtg aacggtgagg cgatacaatg acgaaatccg aatgacgaat
       61 gacgaattaa tgtcgaagct caaatgacga aaagacaggc ggcggcgtcc gtccttattc
      121 gggcttcgtc atttgaattt cctccatcat tcgacattcg tgcttcgtca ttgcagatat
      181 gaagcaatgt atcgcctacc agtagcgaag tttttctcag cggttctatt atttaccatg
      241 gttaatttga agggtgcgcc gagtgaacct tcgtccacga tcacgtttcc gccgagttcg
      301 gcgcaaaaat gggtattgcc caatgggctg acgatcatcg tgcaggaaga tcgcactgcg
      361 ccggtggcaa gtatgcaggc gtggtgcgct accgggagca tcgatgagga tgcgcatctc
      421 ggcgcgggct tgtcccacat tctcgagcac atgctcttta aggggacgaa aacgcgatcg
      481 accaacgaga tcgcgcagaa gatacaagac gtgggcggtt acataaacgc gtatacatct
      541 tttgatcgca cggttttttg gatcgacgtg ccgaaagacg gtgtggctgt cgggctcgac
      601 gtcctggccg atgcaatgat gaattcaact ttgccgccgg aagaatatca gaaagagcag
      661 gaagtcatcc ggcgcgagtt tgccatgggc atggatgatc ccgatcgcat ggccgggctg
      721 cttctttttg cgaccgccta tcagcgacat ccttaccgct tcccggtgat cggcgagatt
      781 gaaatctaca atcagctcac ccaagagcag gtgatgcagt attacaaaac acgctacgtc
      841 ccgaataatc ttacctttat tgtggtgggc gacgtcgatg gcgagaaagt gcggcaacag
      901 ctcactgatc tgttcaagcc ttatccggag aaatcactca agccggtctt catccccgcg
      961 gaaccacccc agctcggccg ccgcgaattg aacaaggaat ttcccactga gctgacgcat
     1021 ctgtcgcttg catggcatat cccggaggtc acgaatccgg atgtgcctgc gctcgatctt
     1081 ctctcgacca ttcttggcga cggacgcagc tcgcgcctct atcggcgcgt gcgcgaagag
     1141 gccggtctgg catatggcat agcggcgttt tcgtacacac ccggcgatcc aggcttattt
     1201 ggcattgatg ccacgcttga tcccaaaaaa cgccaggcag ccgagcaatt ggtcctgcgc
     1261 atcattaacg aggtgaaaca aaccggcgtt acatcggagg aattggcgaa agccaaaaaa
     1321 atgtccctta gccaccatct ggaggccctt accacgatgc gcgggcaggc ttcggacatc
     1381 ggctcgaatt ggttgctcac gcgcaatctc aatttcagcc gggattatct cgatgcggtc
     1441 cagaaagtgt cgctcgatga catcaagcga gtcgccgcaa attacctgac ggacagcaac
     1501 ctgacggtca tatcgctgaa tccaaagggt tcgctggccg ccaaacctga gggcacgaag
     1561 gcggccgcgg cgggtgagat tcaaaaattt gagctctcga atggcttgcg gattctggtt
     1621 cgtgaagatc ggcgtttgcc gctggtcgcg atgggcgccg ttttccgaag cggcctcctc
     1681 gcggagacgg cgcaaaccaa cggtatcacg cgcttaatgg cgaaggtgct gttgaaagga
     1741 accaaaacgc gcaccgcgga gcagatcgca gacgagatcg aaagagtcgg tggctccatc
     1801 gggagtgacg cgggtaataa cagtttcagc gtctcgctcg acgtgatgaa gcccgatctg
     1861 aaactgggcg tggagttgtt cgcggacgtt ttgctcaacg cgaccatgcc ggaaaaagcg
     1921 atcgcacgcg aaaaggaagt ccagatcgcc gccattcaac aagaggaaga gcagttaaca
     1981 gccgtggcgc gcaacatttt gcggcaggcg ctcttccctc aacatcccta tgccctgcgc
     2041 gcaaatggtt tagtcgagtc ggttcagcga ttgaatcaga aggatcttct tgaatttcgc
     2101 gatcgttacc ttgtcgcaaa aaacggcgtc gttttcgtgt ttggcgatgt aaaggcggcg
     2161 gaggtgaagc agctttttga gcaagcgctc ggcggaatga aaccaggcgc gctcgctctg
     2221 acggacgcgc atcccgccgc gccgctcagc aaaatagaaa ccgtcgaaag ccgcaaagat
     2281 aaagcacagg gcgtgatcat ggtcggatat cgcggagcgg atcttttcag tccggaccga
     2341 tacgctctgg agttaatcga cgaggccagc agcgacctcg gctcgcgctt ttttgttcgt
     2401 attcgcgagc aaatggggct ggcctattac gtaggcgcaa gccagatgca gggtctagtg
     2461 ccgggtcttt tcgccttcta tcttggcacc gatccgcaaa aaattgagcc ggtgaaaacc
     2521 gcgttgctcg atgaaatcca caaactcgcc agcgatggcc ttaccagtga ggaactggcg
     2581 cgagcgaaaa agaagctgat cggccagcaa gagatcgcga atcaaagcaa tgctgctttc
     2641 ggttatcatt gcgcgctcga cgagctttac gggcttggtt ttaattatta caagcaactc
     2701 gagcacgacg tgaacgcagt gacgcttgac gaaatcaaac gggtggccgc gaaatatttc
     2761 cgggatcaac cgtatgtttt ggcgacggta cgaccgccag aaggcgccgc agccgcgaaa
     2821 cagaagtgag ccatggctga agtccaaaaa accacgctaa gcggcgagct ttcacaacgc
     2881 ttcattgagt tcgtgatgat gcacgcgcaa aatgccgctt tgtttctcgg tcaaatccca
     2941 aatccaaaaa ccggcgaagg cgaagtgaac ttggagctgg ccaaaatgtt catcgatcag
     3001 ctggccatga ttcaggaaaa aacgcggggc aacctgacaa gcgaagaatc caccgtgttg
     3061 cgcaatgctc tttccaattt gcagatggtt tatgtggaag ttgcgcggga agcgccgaaa
     3121 ggcgctgcgc agccggagca acccgcaccg tcaccaaaac cagcggaaca aacatcgcca
     3181 agcacgccga cgcctgagcc atcagcgccg attagctcga ccgaatccga gacggaatcg
     3241 aaaaagaaat ttaccaagag ctacggcccg taaacgctaa tccacttgcg cgtcatcccg
     3301 agcgcagcga gggacctccc caatctcact tgggacatgc aaattaaaca gcgtgctcaa
     3361 ccgactagtg cgagatcctt cactccgttc aggatgaccg cggccctcca gttttcctga
     3421 ttggatttca acccagcttt ggcaaagtga ttcatggcgt atttcgccgc gattttttgg
     3481 tctataatag aattagaccc aaaaattcgg tataaacctt gttagatgta ttccgctcgc
     3541 ctggtcgcta ttgtctttcc actggcggcg tttgctgcgt gcgtcagtaa tccgcgagtg
     3601 gagcgttcac ctgcaggcat tgcggtcgat ccagagctgt tgtatgcggc cagagaggcg
     3661 catttcgatt acatcaagca gctcgcacgt gcagaagcgg gc
//
LOCUS       DASQUQ010000093        10603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_836800, whole genome shotgun sequence.
ACCESSION   DASQUQ010000093 DASQUQ010000000
VERSION     DASQUQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836800"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VE486_02510"
     CDS             <1..1261
                     /locus_tag="VE486_02510"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZA37991.1"
                     /translation="PMKRKRVVILGATGSIGESALKVARDIPDRMEIVGLAANSNAQK
                     LAAAANEIRPESVCLVDETKIDTLRKALNYEPRIFTGEEGLREIACLTSADMVLVAIV
                     GTGGLRPALAAVEAGKDLAVASKEILVMAGEIVMREARDGGVNVLPVDSEHNAIFQCL
                     EGKRSTPNAQRPTSNAEAPSSTRSSTLSVGRWTLDVSDVRRIILTASGGPFRETPQKD
                     FDSITVEQALKHPTWNMGPKITIDSATLFNKGLEMIEAHWLFGVEMKRVEVIIHPQSI
                     VHSMVEFADGSTLAQLSYSDMCFPIQYAVTWPDRVPNTLPPLDFSKLSKLEFFTPRYD
                     DFPALNLARRAGETGGTLPAVMNAANEVAVAAFLDRKVRFPSIWQIVEEVMNRHASVA
                     HADLDAILQADQWARTEAQGCLKSSHR"
     gene            1326..2741
                     /gene="rseP"
                     /locus_tag="VE486_02515"
     CDS             1326..2741
                     /gene="rseP"
                     /locus_tag="VE486_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZA37992.1"
                     /translation="MLLDIGRVVLILLEVLILFNLLIVVHELGHFLAARWRGLYIEKF
                     GVWFGKPLWKKTINGVQYSLGSLPFGGFVALPQLAPMDIIEGKADLDRAKLPRISALD
                     KIIVAFAGPLFSLLLALFFAGIVWVVGHPVGEADSTTVIGYVLPDSPAQKVGLRPGDK
                     ILEVDGKPVRRFLGMNDSVSWNVVRSEGDKIAVKFQRAGSVHTVWVEPYKAETRGWRR
                     KSVRQLLIYPAETALIDSVEPNTPAAAAGLRKGDIIRGFDQTPIYNPIALLEYISKHP
                     KDQLVLHVERDGKRFDVPVKPTLLPTEGEMKPRIGIRWDTAGIMSISHPNPVEQVYNS
                     VTSTLKTIGAVASPKSDVKLQHLSGPVGIGRIYYLLFQSERGWQLALWFSVILNVNLA
                     ILNMLPIPVLDGGHIVLAIIESVRRKPVNMRVLELVQTSCAVLIIGYMLYITFFDVQD
                     LPFIRNKLDQLEAPAKNTPAP"
     gene            2795..4681
                     /gene="ispG"
                     /locus_tag="VE486_02520"
     CDS             2795..4681
                     /gene="ispG"
                     /locus_tag="VE486_02520"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HZA37993.1"
                     /translation="MNYCADPFHYQRRLTREVKVGDVGIGGDNPIRVQSMITCDTMDT
                     EASIQQTMELAKVGCEIVRITAPTVKDARNLEHIAKGLRERGCNVPIVADIHFKPEAA
                     MEAAKWVDKVRINPGNYADSKKFVIREYTDEQYSAELNRIRERFSPLVDLCRNRGIAM
                     RIGTNHGSLSDRILNRYGDTPLGMVESALEFARIARDLDYHAFVFSMKASNPKIMIAA
                     YRLLVARLNEEGPDWNYPIHLGVTEAGEGEDARIKSAIGIGSLLADGIGDTIRVSLTE
                     DSPHEIPVARALVEMVQNETPDAQYPTFNAQLSFDPFSYQRRATEKMVVAGVDGSGRY
                     VKLGGDELIRVVVRQSNFDKIAHKIDKMGDYKPEIVYEKAQIKELDPRDETAFAQLNQ
                     SSTTQFVTVKDGVDLPVIAAFRLLAARLHPRHPILLKDTLCRVAPGNDDIRMTNDERS
                     PNDRITEGGSGFVIASSFLETLLTASTNIGSLLCDGVGDAILVQGEEAPGQALRLSYN
                     ILQAAGSRIFKTDYVACPSCGRTLFNLQTTTAKIKAATCHLKGVKIAIMGCIVNGPGE
                     MADADFGYVGGAPGKVNLYVGKTAVKFNIPEAEAVDRLQDLIREHGKWVEPARRTTTL
                     EVVT"
     gene            4847..6661
                     /locus_tag="VE486_02525"
     CDS             4847..6661
                     /locus_tag="VE486_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37994.1"
                     /translation="MKTLRRISRNRAFAHVRILVAILLVLAALAIAFMAVSPPAFAQR
                     PARSQPLTPKFSTAVAFDTTRPLRDMAPAPKRFAPSATVLEVRPERGPAIQSKGYSGD
                     GALQPFSPGVAIPGVAIPAPLLTFEGLSNQDNFDVFGFRVNPPDPNGEVGPNHYVEMI
                     NLVFAVYDKNGKKLLGPIDTGSLWAGFAIPDCTDPSGDPVVVYDQFEDRWLLTQFTTR
                     GFNPAGNFNNKPFYNCVAISQTGDPTGAYFRYAFITSGAVDLHGSKSEPFFPDYPKYG
                     VWSNSYLLTSRDFGRKNAYGISVYALEKERMVAGDPNARMVHFFLDSAVVPLSLIGDG
                     LLPADIDGTAQPTEDAPAPIVGTQDDNASYGATFDALNIWELSVQWNANPEASIVLKT
                     QLPVASFDSIFPCAPTDRDCLPQPGITNPAQFLDILSYRQRPTFRLAYRNFGTFEALV
                     TNQSVEALPGVAGARWYEIRRTGGSYSVFQQGTFAPGDGVHRWMGSVAMDKSGDIALG
                     YSVVNGTTVFPGIRYTGRLAGDALGQMTLGEGTIINGSGVQTTTNSRWGDYTDITVDP
                     TDDCTFWYVNQYYTLAGQMSSPAGWQTRIGSFKLPGCE"
     gene            6813..8621
                     /gene="typA"
                     /locus_tag="VE486_02530"
     CDS             6813..8621
                     /gene="typA"
                     /locus_tag="VE486_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZA37995.1"
                     /translation="MDKIRNIAIIAHVDHGKTTLVDQLLRQSGTFRSNQKIEERVMDS
                     MDLEREKGITIRAKNAAFQWNGYRINIVDTPGHADFGGEVERIMKMVDGVLLVVDAHD
                     GPQAQTRFVLRKAMENKLKPVVVINKIDRENARPHRVVDMVFDLFVELKATDEQLDFP
                     IIYASAKNGFAMRELHENNEDMTPLFQAIVQHVPPPKVSTEPFFRMLVSNLDYSDYLG
                     RIALGRIVSGRVAVGDSIVCIHRDGRRERATVTALFTYSGLEQVETKHATAGDIVGLA
                     GFEEVYIGETLTDREERAPLQFVDIDPPTIRMHILVNDSPFAGREGKFVTARHLRERL
                     IRETRGNVSLQVNDTETAGVFEINARGEMQIAILVEQMRREGYELMVSRPEVIFHRAE
                     DGKQLEPLENLYVDLPNENLGDILQSIANRKGEILGMDHHASRVSIEAIIPTRGLIGF
                     ETDLMNLTRGEGLMSHLFREYALFKGEIGGRGRGVMVSMEYGVSTAYALNNIQARGRL
                     FIGPQEDVYEGMIVGENARPEDLPVNPCKAKHLTNMRSQGEGKGIQLEAPLRMTLERA
                     IEYIDIDEYVEATSKSLRLRKRILDATARKRAAAAA"
     gene            8806..10422
                     /locus_tag="VE486_02535"
     CDS             8806..10422
                     /locus_tag="VE486_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZA37996.1"
                     /translation="MKIHHPTSVLSRARLAALGALFVVGASMAAFAAGQGGGASQREH
                     LFVGFTKKPGAAEHALVERYGGKVRFSFPSVNALAIDLDSAKIGVFAREGGISYVEKD
                     PVRTPLGLADAQLTPSLSNGLYGLITTHALEAQAAGFTGAGVKACVADTGLDTTHPDI
                     AANFAAGNDIFGGTNSVDVFKLGVAATETHATHVSGILLGVNNNVGILGVAPQAQLYE
                     ARVLGTQQDGSVSGETSQVMAGVQWLADQGCKVINMSLGGGDRSRAEAALYEQLIANG
                     HMIVASSGNDGSSRPSYPAGYDVVVSVGAVDRNNAHASFSNTGGTVDLSGPGVDVLSS
                     VPKGQGFEASVTTTQTFEAFGLEFAGTTRSRGITKTLVSCGLAVSPSDCPSTVKGNLA
                     LIQRGSVTFAQKVASATTAGAVGAIIYNNAPGNFHGTLGTATNNGKAWIPAVSVSQAD
                     GQTLLSQLPTSATLVNAASSWDFFNGTSMAAPHVSGVAALVFGKNPTLTPFQVESILE
                     STATDLPPAGYDPTFGWGLVNADAALKATP"
BASE COUNT         2293 a         2986 c         3111 g         2213 t
ORIGIN      
        1 ccccatgaag cgtaagcgtg tcgttattct gggtgcgacg ggatcgatcg gcgaaagcgc
       61 gctgaaggtc gcgcgcgata ttccggaccg gatggagatt gtgggactcg ctgcgaacag
      121 caacgcgcag aaactagccg cggctgcgaa tgagattcgg ccggagtcgg tttgtctggt
      181 ggatgaaaca aagatcgaca ccctgcggaa ggcgctcaat tacgagccgc ggatttttac
      241 gggcgaagaa ggtctccgcg agatcgcctg cctgacgagt gccgacatgg tcctggtcgc
      301 aatcgtcggc actggtggat tacggccggc gctcgccgcg gttgaagccg ggaaagatct
      361 cgcggtggcg agcaaagaga ttctggtaat ggcgggcgag atcgtgatgc gcgaagcgcg
      421 cgatggcggc gtgaacgtgc tgccagtgga ttccgagcac aacgcgatct tccaatgcct
      481 cgaaggcaaa cgctcaacgc ccaacgctca acgcccaaca tccaatgcag aggcgcccag
      541 ttcgactcga agttcaacgt tgagcgttgg acgttggacg ttggacgttt ctgatgtccg
      601 gcgcatcatt cttaccgcat ccggcggacc gtttcgcgaa acgccgcaaa aagattttga
      661 ttccatcacg gtggaacagg cgctcaagca tccgacctgg aacatgggac cgaagatcac
      721 aatcgattcc gcgacgctgt tcaacaaagg gctggagatg atcgaggcgc actggctgtt
      781 cggagtcgag atgaagcgcg tcgaagtcat tatccatccg cagagcatcg tgcattcaat
      841 ggtcgaattc gcggacggct cgacgctcgc ccaattgagt tactcggaca tgtgctttcc
      901 gattcaatac gcggtgacgt ggcccgaccg cgtgccgaat actttgccac cgctcgattt
      961 ttcgaaactg tcgaagttag aatttttcac gcctcgttat gacgactttc cggcgctgaa
     1021 tctcgcgcgc cgcgcgggcg agacgggcgg cacgttacct gccgtgatga acgcagccaa
     1081 cgaagtggcg gtcgcagcgt tcctggatcg aaaagtgcgt ttccccagca tctggcagat
     1141 tgtcgaagaa gtgatgaatc ggcatgcgag cgttgcgcat gccgatctcg atgcaatatt
     1201 gcaggccgat cagtgggcgc gcacggaagc gcagggatgc cttaaatcgt cacatcgtta
     1261 gaaaagagtt acaacggcgg agacgatggc gtgtccaatt agctgatcac tagtcactaa
     1321 tcactatgct tcttgatatc ggtcgcgtcg tcctcattct tctcgaggtc ctcattcttt
     1381 ttaatttgct catcgtggtg catgagctcg ggcattttct ggcagcgcgc tggcgcgggc
     1441 tttacataga aaaattcggt gtctggttcg gcaaaccgct ctggaaaaaa acgatcaatg
     1501 gcgtccaata ctcgctcggt tcgcttccct ttgggggatt cgtcgcgctg ccgcaactgg
     1561 cgccgatgga cattatcgaa ggtaaggccg atctggatcg cgcaaagctg ccgaggattt
     1621 ccgcgctcga taaaatcatc gttgcctttg ccggacccct gttcagtctg ctcctcgcgc
     1681 ttttctttgc cggcatcgtc tgggtggttg gccacccggt aggcgaagcg gattcaacta
     1741 cggtcattgg ctatgtcctg ccggattctc cggcccagaa ggttgggctg cgaccgggcg
     1801 ataaaattct cgaagtggac ggcaaaccgg tcaggcgttt tctcggaatg aatgacagcg
     1861 tgagctggaa tgtggtgcga agcgaaggcg acaaaattgc tgtgaaattc cagcgagctg
     1921 gcagcgtgca caccgtttgg gttgagccct acaaagccga gacgcgcgga tggcgcagaa
     1981 aaagtgtgcg gcaacttctg atctacccgg cagagacggc actgatcgac agcgtggagc
     2041 cgaacacgcc cgcggcagcg gccgggctac gcaaaggcga catcattcgc ggattcgacc
     2101 agacgccgat ctacaacccg atcgcgttgc tcgaatacat cagcaaacat ccgaaggacc
     2161 agcttgttct ccatgtggag cgagatggaa agaggtttga cgttccggta aagcctaccc
     2221 ttctgcccac cgagggcgag atgaagccgc gaattggaat tcgatgggac acggccggga
     2281 tcatgtcgat ctcgcatccc aatcccgtgg agcaggttta caatagcgtg acgagcacat
     2341 tgaagacgat cggagccgtt gcgtcgccga aatcggacgt aaagttgcag catttgagcg
     2401 gtccggtcgg catcggtcgt atctattatt tgctcttcca aagcgaacgt ggctggcagc
     2461 tcgctctctg gttcagtgtg attctgaacg tgaacctcgc gattctgaac atgctgccga
     2521 ttccggtgct cgacggcggc cacattgtgc tggcgatcat cgaatcagta cgtcgcaagc
     2581 cggtaaacat gcgcgtcctg gagctagttc agacgagctg tgccgtattg attattggct
     2641 acatgctcta tatcacgttc ttcgacgtgc aggatttgcc gttcatcaga aacaagcttg
     2701 atcagcttga agcgcccgcg aaaaataccc cggcgccgta actaaacccc gactcgttgc
     2761 gcgcctggcc acggttccgt aaattaggcg tttcgtgaat tactgcgctg atccttttca
     2821 ttatcaacgt cgccttaccc gcgaggtgaa ggtgggcgat gtcggcatcg gcggcgacaa
     2881 cccgattcgt gttcagtcga tgatcacctg cgacacgatg gacacggaag cgtcgatcca
     2941 gcagacaatg gagctggcaa aagtgggttg tgaaattgta cgcatcactg cgcctacggt
     3001 gaaggacgcg cgcaatctcg aacacatcgc caagggtctg cgcgagcgcg gctgcaatgt
     3061 gccgattgtc gcggacatcc atttcaaacc ggaagcggcg atggaagcgg caaaatgggt
     3121 ggacaaggtg cggattaacc cgggcaatta cgccgactcg aagaagttcg tcatccgcga
     3181 atacaccgac gagcagtact cggcggagct gaatcggatt cgagagcggt tttcgccact
     3241 tgtggatctt tgcaggaacc gcgggattgc catgcgcata gggacaaatc acggttcgct
     3301 ttcggatcgc atcctgaatc gctacggcga cacgcccctt ggaatggtgg aaagcgcgct
     3361 cgagtttgcc cggattgcgc gcgatctcga ttatcacgct ttcgttttct cgatgaaagc
     3421 gagcaatccg aaaatcatga tcgcagcgta tcgcttgctc gtggcccggc tgaatgaaga
     3481 agggccggat tggaattatc cgattcatct cggcgtgact gaagcgggcg aaggcgaaga
     3541 cgcgcgaatt aaaagcgcga ttggaattgg ctcgctgctg gcggatggga tcggcgatac
     3601 aatccgggtt tcgcttacag aggatagccc tcacgaaatt ccagtggcgc gggcgttagt
     3661 ggaaatggtt caaaatgaaa cgcccgacgc tcaatatcca acgttcaacg ctcaactttc
     3721 gtttgatcca ttttcgtatc agcggcgcgc aaccgaaaaa atggttgtag ctggggtcga
     3781 tggctccggc cggtacgtga aactcggcgg agacgaattg attcgcgtgg tcgttcggca
     3841 atcgaacttc gacaaaatcg cgcacaagat cgacaagatg ggtgattaca agccggagat
     3901 tgtgtacgag aaggcgcaga taaaagaact cgatccgcgc gatgaaaccg cgtttgcgca
     3961 gctaaatcaa agttcgacga cgcaattcgt gacggtgaag gacggtgtcg atcttccagt
     4021 gattgctgcc ttccgtttgc tggcggcccg cttgcacccg aggcatccga tcttgttgaa
     4081 agacacgctc tgtcgcgttg ctcctgggaa tgacgacatc cgaatgacga atgacgaaag
     4141 aagcccaaat gaccgaatta ccgaaggcgg ttcgggtttc gtcattgctt cgtcattcct
     4201 cgaaacgctt ctcaccgcct cgacgaacat cggttcgctg ctgtgcgatg gagttggcga
     4261 cgcgatcctc gtgcagggcg aggaagcacc cgggcaggcg ttgcgcttgt cctacaatat
     4321 tctacaggct gccggatcgc gcattttcaa aaccgattac gtggcgtgtc catcgtgtgg
     4381 gcgcacttta tttaacctgc aaaccaccac ggcgaagatc aaagctgcga catgccatct
     4441 caaaggcgtg aagattgcca tcatgggctg cattgtgaat ggacctggcg aaatggcgga
     4501 cgcggatttt ggttacgtgg gcggcgcgcc cggcaaggtg aatctctacg tcggcaagac
     4561 agcggtgaag ttcaatattc ccgaagcgga agcggtggat cgcttgcagg atttgatccg
     4621 tgaacacggc aaatgggtcg agcctgcacg gcggacgact actctcgaag ttgtcacgtg
     4681 agcccgctcc ttaaagacac ctcctcggtg tcttttcatt tgagtcaatc cgctcaatca
     4741 aaaaaagaaa aaaatcttga cgtctttttc gtgattctgg caaactgcgc ttctcttgtg
     4801 tcggggaagt aaatccaaac ttcaacttct aacccaaata aaaaatatga aaactctccg
     4861 tcgaatcagt agaaatcgtg cgtttgcaca tgtacgtatt ttggttgcga ttcttcttgt
     4921 gttggccgcg ttagcaatcg ctttcatggc cgtgtcgcca cccgcatttg ctcagcggcc
     4981 tgctcgatcg cagccgctaa ctccaaaatt ctccaccgct gtggcttttg atacgacacg
     5041 cccgctgcgt gatatggcgc cagcgcctaa gcggtttgcc ccctcagcca cagtactgga
     5101 agtgcggccg gagcgaggac cagcgatcca gtccaagggc tacagtggcg atggagcact
     5161 gcagccgttc agccccggcg tggccatccc cggggtagcc atccccgctc cgttgctgac
     5221 tttcgaagga ctgtcgaatc aggacaattt cgacgtcttt ggtttccggg ttaatccgcc
     5281 cgaccccaac ggagaggtcg gcccgaacca ctacgttgag atgattaacc tcgtcttcgc
     5341 cgtgtacgac aagaacggca agaagcttct cggaccgata gataccggtt cgctctgggc
     5401 gggctttgcg attcctgatt gcaccgatcc ttccggcgat ccggtggtgg tgtatgatca
     5461 atttgaggat cgctggctcc tgacccagtt cacaactcgc gggttcaatc cagccggcaa
     5521 tttcaacaat aaacctttct ataactgcgt cgcgatctcg cagaccggag atcccaccgg
     5581 cgcgtacttc cgctacgcgt tcatcacctc cggcgctgtt gacctgcacg gtagtaagtc
     5641 cgagccgttc ttccccgact acccaaagta cggagtttgg agcaactcgt atctgctcac
     5701 gtcgcgcgat ttcggacgca agaatgcata tggcattagc gtttacgcgc ttgaaaaaga
     5761 aaggatggtc gcaggagatc ccaacgcgcg catggtgcac ttcttcctcg attcggcggt
     5821 ggtgccgctt tccctgatcg gcgatggcct gctcccggcg gacattgacg gcacagccca
     5881 gcccacagag gacgctccag ccccgatcgt cggtacgcag gacgacaacg cctcttacgg
     5941 cgcgacattc gacgcactca acatctggga actcagtgtg cagtggaatg ccaatcccga
     6001 ggcatccatc gtcctcaaga cgcaacttcc tgttgccagc ttcgactcga tcttcccgtg
     6061 cgccccgaca gaccgcgact gcctgccaca accgggaatc accaaccctg cacagttcct
     6121 cgacatcctt tcctaccggc agcggccgac gttccgcttg gcttatcgca attttggcac
     6181 gttcgaggca ctcgtgacca accagtccgt agaggcactg ccgggcgttg ccggcgcgcg
     6241 ctggtacgag atccggcgga cgggcggcag ctactcggtc ttccagcagg gaacgtttgc
     6301 acccggtgac ggagtgcacc gttggatggg cagcgtcgcc atggataaga gcggcgacat
     6361 cgctttgggc tatagcgtag tgaatgggac gaccgtgttt ccaggcattc ggtacaccgg
     6421 ccggctcgct ggcgatgcgt taggccagat gacgctgggc gaaggcacga tcatcaacgg
     6481 ctcaggcgtg cagacgacca cgaactcgcg ttggggtgac tacacggata taacagtcga
     6541 tcccaccgac gactgcactt tctggtacgt gaatcagtac tacacgctgg cggggcagat
     6601 gtcctcccct gcagggtggc agacgcgaat cggcagtttc aaactccctg gttgcgagta
     6661 gcagtaatca cacgacggcc tgcagcgagc gtcatgtctg ctgaagacat tccgcgctcg
     6721 atttccggca atatttgtga cgccgaggtt tgactcgggc gtcgtacctc gtaagtttcg
     6781 cggctcttcc gctcgggaga gattctcttt ccatggataa aatccgtaat atcgctatca
     6841 ttgcgcacgt cgatcacggc aaaacgacgc tggtggatca attgctgcgg caatcgggga
     6901 cgtttcgctc caatcaaaag attgaggagc gggtgatgga ttcgatggat ctcgagcgtg
     6961 agaaaggaat cacgattcgc gcgaagaacg cggcgttcca atggaacggt tatcgaatta
     7021 acattgtcga cacgcccggt cacgctgatt tcggcggtga agtcgagcga atcatgaaaa
     7081 tggtggacgg cgtgttgctc gttgtcgatg cgcacgacgg gccgcaggcg cagacgcgct
     7141 ttgttttgcg caaggcgatg gagaacaaac tgaagcccgt cgttgtcatc aacaaaatcg
     7201 acagagaaaa cgcgcgcccg catcgagtcg tcgacatggt tttcgatctt ttcgttgaat
     7261 tgaaggcaac cgacgagcaa ctcgatttcc caatcatcta tgcgagcgcg aagaatggtt
     7321 tcgccatgcg cgagctacat gaaaataacg aagacatgac gccgcttttt caagcaatcg
     7381 tgcaacatgt gccgccgcca aaggtttcga ccgaaccgtt cttccggatg ctcgtgtcga
     7441 acctggatta cagcgattat cttggacgga tcgcgctcgg gcgcatcgtg agcggtcgcg
     7501 ttgcggttgg cgattcgatc gtctgtattc accgtgatgg ccgccgggaa cgagccacgg
     7561 tcacggcgct tttcacctac tcgggattgg agcaggtcga aacaaaacat gcgaccgccg
     7621 gtgacatcgt ggggctcgca ggtttcgagg aagtttatat tggtgaaacg ctgacggacc
     7681 gcgaagaacg cgcgccgctt cagttcgtgg atatcgatcc gccgacgatt cgcatgcaca
     7741 ttctggtcaa cgattcgccg tttgccggtc gcgaaggaaa atttgttacc gcgcggcacc
     7801 ttcgcgaacg gcttattcgc gagacgcgcg ggaatgtttc gctccaggtg aacgacacgg
     7861 aaaccgcagg cgtatttgag attaacgcac gtggcgagat gcaaatcgcg atcctggtcg
     7921 agcagatgcg acgggaaggt tacgaactaa tggtctcgcg tccggaggtg atttttcacc
     7981 gcgcagagga tggcaagcag ctcgagccac tcgaaaatct ctatgtcgat ctaccgaacg
     8041 aaaatctcgg cgacattttg cagtctatcg cgaatcgcaa aggcgagatt ctcggcatgg
     8101 atcatcacgc gtcgcgcgtt tcgattgaag cgatcatccc cacgcgcggc ctgatcggat
     8161 tcgaaaccga tctgatgaac ctgacgcgcg gcgaaggctt gatgagtcat ctttttcgcg
     8221 aatatgccct cttcaaaggc gagattggcg gacgcggtcg cggcgtgatg gtgtcgatgg
     8281 aatacggagt cagcactgcg tacgcgctca acaatatcca ggcacgcggc cggctcttta
     8341 tcgggccgca ggaagacgtt tacgaaggaa tgatcgtggg cgagaacgcg cgcccggaag
     8401 atttgccggt gaatccctgc aaggcgaaac atctaaccaa catgcgttcg caaggtgaag
     8461 gcaaaggcat tcaactggaa gcgccgctcc gcatgactct cgagcgcgcc atcgagtata
     8521 tcgacatcga cgaatacgtc gaagcgacat cgaaatcttt gcgtttgcgc aagcgcatct
     8581 tggacgcaac cgcccgtaag cgcgctgccg ctgcggcgta gcttgttcca ggcgaaagcg
     8641 ggcgcgccga agggcaatcg ccttgcctgg tgttaacaag tcagactctg acctaaggaa
     8701 tttaaaaaaa aaaacgacca aggcgcttga cgtatccgtt tcttattcga gtctcgtatt
     8761 ctctacattc ggcaaacttt aacctctaac tcgtagctaa aaaaaatgaa aatccatcat
     8821 ccaacctctg ttctatcacg cgcgcgcctg gcggcactgg gcgcgctttt cgttgtcggt
     8881 gcttcgatgg ctgcctttgc tgctggccaa ggcggcgggg cgtcgcagcg ggagcacctg
     8941 ttcgtcggct tcaccaaaaa accgggggcc gccgagcatg cgctcgtcga gcggtacggc
     9001 ggtaaagttc gtttctcatt cccatcggtt aatgcgcttg cgatcgatct cgatagcgcg
     9061 aagatcggcg ttttcgctcg cgagggcggg atttcgtacg tcgagaaaga tcctgtccgg
     9121 acgcccctcg gcctggcgga tgcgcagctg acgccgtcgc tctcgaacgg gctctacggg
     9181 ctgatcacga cccacgccct cgaggcgcag gcggcaggct tcaccggcgc aggcgtcaag
     9241 gcctgcgtcg ctgacaccgg tctcgacacg acgcacccgg acatcgccgc caatttcgcg
     9301 gcgggcaacg atatcttcgg cggcaccaac agcgtggatg tgttcaagct tggcgtggcc
     9361 gcgacggaga cgcacgcgac ccacgtctcc ggtatcttac tcggcgttaa caacaatgtc
     9421 gggatcttgg gtgtcgcacc ccaggcgcaa ctttatgaag ctcgcgtgct cgggacgcag
     9481 caggacggga gcgtcagcgg agagacgagc caggtcatgg ccggcgtgca gtggctcgcc
     9541 gaccagggct gcaaggtgat taacatgagc cttggcggcg gcgaccgctc gcgcgccgag
     9601 gcggcacttt acgagcagtt gatcgcgaac ggccacatga tcgtcgcctc ctccgggaac
     9661 gacggctcga gccgcccgag ctatccggcc ggctacgatg tggtcgtgtc ggtaggggcg
     9721 gtcgaccgga ataacgccca tgcgagcttc tcgaacacgg gcggcaccgt cgacctgtcc
     9781 gggcctggcg tcgacgtgct ctcctccgtc ccgaagggcc agggcttcga ggcatcggtg
     9841 acgacgacgc agacgttcga ggcgttcggt ctcgagttcg caggcacgac gcgctcgcgc
     9901 ggcatcacga agacgctcgt cagctgcggc ctcgcggtgt cgccatctga ctgcccgtcc
     9961 accgtgaagg gcaacctcgc gctcatccag cgcggctcgg tcaccttcgc ccagaaggtg
    10021 gcgagcgcaa cgaccgccgg cgcagtcggc gcgatcatct acaacaacgc gcccggtaac
    10081 ttccacggca cgctcggtac cgccaccaac aacggcaagg cgtggatccc ggccgtttcg
    10141 gtatcgcagg ccgacggcca gacgctgctg tcgcagctcc cgaccagcgc gacactggtg
    10201 aacgccgcgt cctcgtggga cttcttcaac ggcacgtcga tggcagcgcc gcatgtctcc
    10261 ggcgtagcgg cgctcgtgtt cggcaagaac ccgaccctca cgccattcca agtcgaatcc
    10321 atcctcgaga gcacggccac cgacctcccc cccgccgggt acgacccgac ctttggctgg
    10381 gggctcgtga atgcggacgc cgctctcaag gcgacgccgt aggaattaaa taatcgcaaa
    10441 tcaattctag gagacaggga cgcggataaa accgcgtccc tgtttttttg taaggacggc
    10501 gcgctgcggt ccgccgcggc ggtacccgtc gcgcagaact tttccattgg cggggttcaa
    10561 acctagaatc gaacggcctc acaattcacg actcaacatc ggg
//
LOCUS       DASQUQ010000094         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_558912, whole genome shotgun sequence.
ACCESSION   DASQUQ010000094 DASQUQ010000000
VERSION     DASQUQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558912"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VE486_02540"
     CDS             complement(<1..1065)
                     /locus_tag="VE486_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZA37997.1"
                     /translation="MGVILQAFYWDCPKAENREHQWWVLLKSKLPSIAQAGFTALWLP
                     PANKAAGWKSMGYDPYDYYDLGEFDQKGGIPTWFGSKAQLLDLIQSAHSAGLQVYADL
                     VFNHNSGADAQESNPIDGQWRWTKFAPKSGKFSRDWTCFHPNRYETWDGTTFGDMPDL
                     CHRAPAVYTELISYARWLLEEIGFDGFRYDMVKGYGGWMVRSIQELRALRGGTSFKPY
                     AVGECWDSERTIGDWLDEANAWSDNPVGAFDFPLRWRLRDLCDSYGFSLRNLAEREVL
                     MWNRPDQAVTFVENHDVVRDSPIINDKLLAYAFILTHGGYPCVFWQDYFNWDLAQPDN
                     QSGIDALIKVHEQNAAGPVQV"
     gene            1298..2122
                     /locus_tag="VE486_02545"
     CDS             1298..2122
                     /locus_tag="VE486_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZA37998.1"
                     /translation="MISGAKPGEHAEDETTEPAGVTFAPAPDVGGLWAEPANSLPERA
                     ILYLFGGGYMLGSPASRRKTAGHLSVAAKARVLVPNYRLAPEHPFPAAVDDAVRAYEW
                     LLAHGGEPSKTVVAGDSSGGGLAVSTVIAARDRGLRMCAGVIALSPWADLTCSGESTT
                     SRAATDLECTRAGLLEMAGWYLHGADPRQPLASPVFADLTGLPPLLCLVGGDEILLDD
                     TVRLVRGAGMAGADATGFIAAGMQHVFPIWAGVFPEANAAVALIGNWVRARTEHSA"
     gene            2228..3637
                     /locus_tag="VE486_02550"
     CDS             2228..3637
                     /locus_tag="VE486_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA37999.1"
                     /translation="MKEETIDTHTKALKINLDPRWYGTFAEIGAGQEVVRWFFRVGGA
                     AGTIAKSMSAYDMKVSDAVYGRAERYVSRGRLQAMLDREFDLDVERLAKERGDNTSFF
                     AFADTVVARSYRGTNECHGWMGIKFQSRVNDQPSQIVMHVRMLDAEASLQQEALGIVG
                     VNLCYGAFFLSHVPEELVESLLDKLTTGRVEIDMIELSGIEFRNVDNRIMALKLVQLG
                     LSGAAMFGPNREVLQPSDVLHKKAVLVERGSFRPMTNVNLDMLQCALAKFKEDPAVAD
                     KPILPLMELTMRNLLAGGTVVDRRDFLSRAELLAACGMTVLISDYFEYYRLAAYLAWR
                     TNERIGIVMGLPSVSELFDEKYYAQLPGGTLESFGRLFKNDLKVYVYPLQRDEGDELQ
                     TIHTLKVEPDLQPLYDYLVGRGSFVQLDNFNPKYLPIFSRDVLKRIASGDESWDEMVP
                     VKVAEIIRQRGFFGYKKKE"
     gene            3742..>4022
                     /locus_tag="VE486_02555"
     CDS             3742..>4022
                     /locus_tag="VE486_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38000.1"
                     /translation="MNDCDGYAGPVRALAGSAGIQFDELQAEVYRLRDQLGPDHKKAY
                     EESIRRLARGGAISDTEQRALLELGKRGFAVGSDAGFEKFRHYAQALHKR"
BASE COUNT          857 a         1107 c         1203 g          855 t
ORIGIN      
        1 cacctgtacc gggccggcgg cattttgttc gtgaacttta atgagcgcat caatcccgct
       61 ttgattgtcg ggctgagcga gatcccagtt gaagtaatcc tgccagaaaa cacaagggta
      121 acctccgtgt gtgaggatga aggcgtaggc tagcagcttg tcgttaatga tggggctgtc
      181 acgcaccacg tcgtggttct cgacgaacgt gacggcctgg tcggggcggt tccacatcaa
      241 aacttcgcgc tcggcgaggt tgcgcaaact gaaaccgtag ctgtcgcaca aatcacgcag
      301 gcgccagcga agcgggaaat caaacgcgcc gacgggattg tccgaccacg cgttcgcttc
      361 atcgagccaa tcaccaatcg tgcgttcgct gtcccagcat tcgccgaccg catacggctt
      421 gaaactggta ccaccgcgca atgcgcgcaa ttcctgaatc gaacggacca tccagccacc
      481 gtaacccttg accatgtcgt agcggaatcc atcgaaacca atttcttcca ggagccagcg
      541 ggcataactg atgagctcgg tataaactgc gggagcgcga tgacaaagat ccggcatgtc
      601 accgaacgtc gtgccgtccc aggtctcgta ccggttcgga tgaaagcagg tccagtcacg
      661 cgagaatttg ccactcttcg gtgcaaactt ggtccatcgc cattggccgt cgatcgggtt
      721 cgactcctgg gcgtcagcgc cactgttatg attgaatacc aggtcagcat acacttgcaa
      781 tcccgctgag tgagcggact ggatcaggtc gagtaattgg gctttggaac cgaaccacgt
      841 ggggatgccg cctttctgat cgaactcgcc gaggtcgtaa taatcgtaag gatcgtagcc
      901 catcgatttc catccggccg ccttattcgc gggcggtagc cacagggccg tgaaacctgc
      961 ctgcgcaatc gaaggcaatt tggatttgag aagtacccac cactggtgct cgcgattttc
     1021 cgctttcgga cagtcccaat aaaacgcttg cagtatgacg cccatgattg cgctccgttt
     1081 ctgtgctaag aagcgtcgcc acgttacacc gcgcgaacat tgactaacaa gcagcaatca
     1141 gaatcgccgc tgaaaaattc aaagatatga ttgcgccaaa gttttacgcg aaagcatagc
     1201 tcgataagct gcgaaagtgg agacgatgtg atcagcaagc aggcgcagga attctggagc
     1261 ctcctcaaaa gcgcgcccaa acaaatcgac cttccgattg atcagcggcg cgaagcctgg
     1321 cgaacacgcg gaagacgaaa ctacagaacc ggctggagtc actttcgcgc ctgcacccga
     1381 tgttggcggt ctttgggctg aacctgccaa ttcactgcct gaacgggcga tcctctattt
     1441 atttggtgga ggatatatgc tcggctctcc agcttcgcga cgaaagaccg ccggtcatct
     1501 ctccgtcgcg gcaaaagcgc gcgtactcgt gccgaattac cgcctcgcgc ccgaacatcc
     1561 atttccggct gcggtcgatg atgcggtaag agcttatgaa tggctcctcg ctcacggtgg
     1621 agaaccatcg aaaactgtgg ttgccggcga ctcgtctgga ggcggtcttg cggtttcaac
     1681 agtcattgcc gcgcgtgatc gtggcctgcg aatgtgcgcg ggagtgatcg cactctcgcc
     1741 ctgggccgat ctcacatgta gtggcgagag cacgacgagc cgcgctgcta ccgacttgga
     1801 atgcacacgc gcgggtctct tggagatggc gggatggtac ctgcacggcg ccgatccgcg
     1861 tcaacctctc gcctcgccgg tgttcgccga tcttaccggg cttccgccgc ttctctgttt
     1921 ggttggcggc gatgagatac tcctcgatga tacggttcgc ctggtgcgag gcgcggggat
     1981 ggcgggcgcg gacgcaaccg gttttatcgc ggcgggaatg caacacgtat tcccaatctg
     2041 ggccggcgtc ttcccagagg cgaacgccgc ggtcgcattg attggcaatt gggtgcgagc
     2101 gcgcactgag cacagcgcct aacgagtctg tggatcactg cggcggtcat gccgcgcttt
     2161 gcgttgcgga gattgatgca ctatctaaat cttcaaggct cgggcgacgg cctgactctg
     2221 ctgcgacgtg aaagaggaaa cgattgatac gcatacgaag gcgctgaaga tcaatctgga
     2281 tccgcgctgg tacggaacgt tcgcggaaat cggcgccggg caggaggtgg tgcgctggtt
     2341 cttccgcgtc ggcggcgcgg cgggaacgat cgcgaagagc atgtccgctt atgacatgaa
     2401 agtgagcgac gccgtctatg ggcgcgcgga gcgttatgtc tcgcgcgggc ggctccaagc
     2461 gatgctcgac cgggagtttg atcttgatgt ggagcggctc gcgaaagagc gcggcgataa
     2521 cacttcgttc ttcgcatttg ccgatacggt ggtggcgcga agctaccgcg gcactaacga
     2581 atgtcacggg tggatgggaa ttaaattcca gagccgcgtc aacgatcaac cgagtcagat
     2641 cgtgatgcac gtgcggatgc tcgacgcgga ggcgtcgctg caacaggagg cgctcggcat
     2701 cgtgggagtc aatctttgct acggcgcgtt ttttttgagc cacgtgccgg aggagttagt
     2761 cgagtcgctg cttgacaagc tgaccaccgg ccgcgtcgag atcgacatga tcgaactcag
     2821 cgggatcgaa tttcgcaacg tggacaatcg catcatggcg ctgaagctcg tgcagctggg
     2881 gctgagcggc gcggccatgt ttggcccgaa tcgcgaggtg ctgcagccgt cagacgtgct
     2941 ccataaaaag gcggtcctgg tcgagcgcgg gagttttcgg ccgatgacga atgtcaatct
     3001 cgacatgctg cagtgcgcgt tggcgaagtt caaagaagac cctgcggtag ctgacaaacc
     3061 aatcctgcca ctcatggaac tcacgatgcg taacctgctg gcgggcggca cggtggtgga
     3121 ccgccgcgat tttctctcgc gcgcagagct gctcgcggcc tgtgggatga ctgtgctgat
     3181 ttccgactac ttcgaatact atcggctcgc tgcctatctc gcctggcgga caaacgagcg
     3241 catcggcatt gtcatgggtt taccgagcgt ttccgagtta tttgacgaaa agtattatgc
     3301 gcaactgccc ggcggtacgc tggagagttt tggccggctg tttaagaatg acttgaaagt
     3361 ctatgtctat cccctgcagc gtgatgaagg ggacgagtta caaaccatcc acacgctcaa
     3421 ggtggaaccc gacttgcagc cgctctatga ctacctggtg ggccgcggca gcttcgtgca
     3481 gctggataat ttcaacccga aatatttgcc gattttttcg cgtgacgtac tcaagcgcat
     3541 cgccagcggc gacgagagct gggatgagat ggtgcccgtg aaagtagctg agatcatccg
     3601 ccagcgcggc tttttcggct acaaaaaaaa ggaatgatct tgtctttggt cggaaaggaa
     3661 taacggggca tttgattttt ttcattgttc cccatgtcac tggatcgtta tccttcggtt
     3721 caccgtgaaa agcaaactcg tatgaatgat tgtgatggat atgcaggtcc cgttcgagcg
     3781 ttagcaggct cagccggaat tcagttcgat gaacttcagg ccgaagtgta tcgactacgc
     3841 gatcagcttg gccccgacca caagaaagcc tacgaggaat ccattcgccg actggcccgc
     3901 ggcggagcta tctcggacac cgaacagcgc gcgctgctcg aactggggaa gcgcggcttc
     3961 gccgtcggct ccgacgccgg gttcgaaaag tttcgccatt acgcgcaggc cttgcataaa
     4021 cg
//
LOCUS       DASQUQ010000095         7230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_106384, whole genome shotgun sequence.
ACCESSION   DASQUQ010000095 DASQUQ010000000
VERSION     DASQUQ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106384"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /gene="trmD"
                     /locus_tag="VE486_02560"
     CDS             <1..487
                     /gene="trmD"
                     /locus_tag="VE486_02560"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZA38001.1"
                     /translation="KVVLMSPAGRRFDQQMAAELSGESHLIIICGHYEGVDHRVIEHL
                     VDAEISVGDYVLTNGAIAAVVLVDAIVRLLPGVLGHEQSAVDDSFSRGLLEAPQYTRP
                     AEFRGWKVPDVLLSGNHAEIEMWRKKQALNRTRENRPDLLDNFDNKRGHSKRSEGPLT
                     G"
     gene            complement(606..1853)
                     /gene="fabF"
                     /locus_tag="VE486_02565"
     CDS             complement(606..1853)
                     /gene="fabF"
                     /locus_tag="VE486_02565"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:TIGR03150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZA38002.1"
                     /translation="MSRRRAVITGIGPITCIGHGREDFWNGILAKKSGINTITSFDPG
                     AFRVRCAGEIRDWDPEKFFPAHRLKRLDRYAQFAVASAQLALDDAHFNYSRETPQHRV
                     GVSFGTALGGVCKAEDQYIRFLKKGARGIQPTLAVQVFGGSAHSNIAIDFGFRGVGTT
                     NSNSCASGTVSLGEALRYIRDDFADVIIAGGAEAPLTTITIGAFDIIKTMSRWSGDPA
                     LACRPFDLLRDGFVMGEGAASLIIEELEHARARGARIYAEVLGYSLNNDAFHMTSPLP
                     SGESCVRAMRDALADARLAPEQIDYINAHASSTQLNDSAETMSIKQVFGEHAKRIPVS
                     GTKGYYAHPLGATGAIEAALCALALDRQWIPPTINYENPDPACDLDVVPNHGREAKVN
                     YVMSNSFGFGGINACVVLGRVRD"
     gene            complement(1850..2305)
                     /locus_tag="VE486_02570"
     CDS             complement(1850..2305)
                     /locus_tag="VE486_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZA38003.1"
                     /translation="MHKTNSIIMRAPKMSIFETAANLELWLKILPHYRYIRYLERSPD
                     RNVVVMAATRSGIPISWTSEQIIDREKIEVRFHHLKAFTKGMRVVWTFKETPEGVLVE
                     IAHDLRFRIHFLAPIADKIIGDFFIHHIANKTLRCMKAHVEAQAKPVTA"
     gene            complement(2463..2684)
                     /locus_tag="VE486_02575"
     CDS             complement(2463..2684)
                     /locus_tag="VE486_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38004.1"
                     /translation="MEGNELWVGNFGDGQINNYDPVTGAFVEALVTADGTPLEFEGLW
                     DLLPLGEGVFFTAGIADEEHGRFGVITED"
     gene            complement(2986..4158)
                     /locus_tag="VE486_02580"
     CDS             complement(2986..4158)
                     /locus_tag="VE486_02580"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZA38005.1"
                     /translation="MKTHNSIANNFTESQNLPLEDNLEERKSPISRRSFLGKSLAVGA
                     GTIGAGLLANVPTAEARGGLTRGDAALLRFAAAAEILEADFWIQYNELGGIQDDEVPG
                     GSGNPDYTEALEVIEDEMGDYIHDNTDDEITHHQFLNAYLVSKGAAPVNLDPFRTLMG
                     STATGVNPALIGRRLTNLTQLTIDTSWWTRYRDDSHNPDLDPNFTFPQAVPTLGVNRH
                     TAIPRTDADTSDPNFLQAIANTAAFHFPTIEQGGNSLYPAMALRATRKEVLRILISIG
                     PTETMHFQTWSDVAGNAPPLTAVDPVTGVSVTFPDLEVEDELFDKALIMPEPCPFLDR
                     SLPVCSIIRPTKTRGVAIGTLQFLTEMGLFIGQSPAFFALMIQLAQDADNARHGRR"
     gene            complement(4323..5018)
                     /locus_tag="VE486_02585"
     CDS             complement(4323..5018)
                     /locus_tag="VE486_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZA38006.1"
                     /translation="MRILVVEDEVQLTRQIASALIQAGHDPVIVHDGERALRETTDTR
                     FDLIVLDIGLPRMDGFEVLRHLHSRHVPTRVLMLTARGEVKDRVTGLQLGADDYLPKP
                     FAMLELVARVNALGRRYPEEPTLNLRVGDLTFDLANHEVYRGTQRIELSARELLLLKV
                     MMREPGRVFTRTELCERVWEHEHEYDTKLVEVFIGRLRKKIGDPPLIHTVRYVGYTIR
                     EPGKSHLAHETDE"
     gene            complement(5041..6261)
                     /locus_tag="VE486_02590"
     CDS             complement(5041..6261)
                     /locus_tag="VE486_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38007.1"
                     /translation="MRLLRALSFCLFACTVRAFDIDDFLDRLDSTLTVSAFQDNLRTR
                     LSGTLDLEIYHFEQPAPGLINSDIDTLFNPRLTLFLDAQLGSQFYFFAQSRLDRGFDP
                     SDRGAQVRLDEYALRFTPWDDGRFNLQVGTFATVVGNWVPRHLSWENPFINAPLIYEN
                     VTPIQDKSAPFSPVDFIYGLYNYEKYEFNPVIWGPSYASGISVSGRIGRFDYAAEMKN
                     ASLSSRPESWHVTAIGFENPTFNARLGFRPNEAWNFGLSASEGAYFRPEAERTLPRGR
                     GIDDYREFVLGQDVSFAWHHLQLWAEFYEARFEVPRVGEVDTFAYYLEIKYKISPQLF
                     GALRWNQQFFNSINDGAGGHLRWSEDLARIDVAAGYRFTSHTQLKLQYSFQHETTGPR
                     NDNHLLGAQLTVRF"
     gene            complement(6267..>7230)
                     /locus_tag="VE486_02595"
     CDS             complement(6267..>7230)
                     /locus_tag="VE486_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZA38008.1"
                     /translation="FYKRLNPDSLFPPAYEICIYPLTDSMAQLNRLRWQIGGAGVLLL
                     LGGFVASHLIAGRLARPVEKLALDSEENRAERKRAEAALASTTEELERSTRYSADASH
                     QLKSPVTVLRLGLEGLLHRDGFKSEIYEELSALLHHTHRLTGVIDDLLLLSRVDAGHL
                     ELESNPVNLSQIIEEWLDDLGALPDSPEMKIEKQFPADLCVAGEKRYTSLIVQNLLEN
                     ARKYNRPGGLIRVTALTSKGEVVLTVGNTGHPIAHDAQEHIFERFHRGGTGSHISGHG
                     LGLNLARELARLHNGDLRLVRSEDDWTEFEARFLIAAQTSDGAT"
BASE COUNT         1644 a         1983 c         1968 g         1635 t
ORIGIN      
        1 caaggttgtt ctcatgtcgc ccgcaggacg ccgttttgat caacagatgg cggcagaact
       61 ttcgggcgaa tctcatctta tcatcatctg tggtcattac gaaggagtcg atcaccgcgt
      121 gatcgaacac ctcgtggacg cagaaatctc cgttggcgat tatgtcctca ccaacggcgc
      181 gattgcggct gtggtcctgg ttgatgcaat cgtgcggttg ctgcccgggg ttctaggcca
      241 cgagcaatcg gcggtggacg atagtttcag tcgcggatta ctggaagcgc cgcagtacac
      301 gcgcccggcg gagtttcgcg gctggaaagt cccagacgtt ttgctctctg gaaatcatgc
      361 cgagatcgaa atgtggcgaa agaagcaggc gctcaaccga acgcgggaaa atcgcccgga
      421 tttactcgac aacttcgaca ataaaagggg tcattctaag cgaagcgaag gacctctcac
      481 agggtaattg cgccttcaaa ttactgcgcg catcgctcgc ttcgattgtg aggtctctcg
      541 tcgtcggagc ctgtcctgag cgagcgcagc gagtcgaacg ggcgactcgg gatgaccgcg
      601 gtttattagt cgcgcacgcg ccccaacacc acgcaggcgt tgattccgcc gaatccgaat
      661 gaattgctca tgacataatt caccttcgct tcgcgcccgt gatttggaac aacatcgagg
      721 tcacacgccg gatcgggatt ctcgtaatta atcgtcggcg gaatccattg gcgatccagc
      781 gccagcgcgc aaagtgccgc ctcaatcgcg ccggtcgcgc cgagcggatg ggcgtaataa
      841 ccttttgttc cactaaccgg aatccgcttt gcatgttcgc cgaacacttg tttgatcgac
      901 atggtttcgg cgctgtcatt cagctgcgtc gagctggcgt gcgcgttgat ataatcgatt
      961 tgctcgggcg caagccgagc atcggccagc gcgtcgcgca tggcgcggac gcacgattcg
     1021 ccgctcggca gaggcgatgt catgtggaaa gcatcattgt tgagactata gccgagcact
     1081 tctgcataga tgcgggcgcc acgcgctcgg gcatgctcga gctcttcgat gatgagcgag
     1141 gccgcgcctt cgcccatgac gaatccatcg cgcaacagat cgaacggacg acaagccaga
     1201 gccggatcgc ccgaccaacg gctcattgtt ttaatgatat cgaatgcccc gatcgtaata
     1261 gtcgtgagag gcgcttcggc tcctccggcg ataataacgt cggcaaaatc atctcgaata
     1321 taacggagcg cttcacccag cgataccgtg ccgctcgcac aactgtttga attcgttgtg
     1381 cccacgcctc gaaaaccaaa gtcaatcgcg atgttcgaat gagctgagcc accaaaaacc
     1441 tgcacggcaa gcgtgggttg aattccacgt gcgccttttt ttaggaaccg gatgtattga
     1501 tcttcggcct tgcagacccc gccaagagct gtgccaaaac ttacgcccac ccgatgttgc
     1561 ggcgtttcgc gggaataatt gaagtgcgcg tcatcgagcg ccagctgcgc cgatgccaca
     1621 gcgaattgcg catagcggtc gagtcgtttt aaacggtgcg ccggaaagaa tttctctgga
     1681 tcccaatcgc gaatctcgcc ggcgcagcgt acgcggaagg cgccgggatc aaagcttgtg
     1741 atggtgttta tgccgctctt cttggcgaga atcccgttcc aaaaatcttc ccgcccatgt
     1801 ccgatgcagg tgatgggccc aatcccggtg atgacggcgc gtctgcggct catgcggtca
     1861 ccggtttggc ttgggcttcg acatgtgcct tcatacagcg caacgtcttg tttgcaatgt
     1921 ggtgaatgaa gaaatcgcca atgattttgt ccgcgattgg cgcgaggaaa tgtattcgaa
     1981 atcgcaaatc gtgcgcgatc tccacgagaa cgccttccgg agtttcctta aatgtccaaa
     2041 ccacgcgcat gccttttgtg aatgccttca agtgatgaaa acgcacttcg attttttcgc
     2101 ggtcgatgat ttgctccgat gtccaggaaa tgggaatgcc ggaacgcgtc gccgccatca
     2161 cgactacatt gcgatccggg ctgcgctcca aataacgaat gtagcgataa tgtggcaaaa
     2221 tcttcagcca cagttccagg ttcgccgccg tctcgaagat cgacatcttc ggcgcgcgca
     2281 taatgatgga attggtttta tgcattcagg ttgctgcgga atctacacgc gcggatgggg
     2341 agcgcacgtt tcgagcgtgt ccgccgtagt atccatccgc gaagatcggc gtccggcagg
     2401 atgccgaacg cagcatccaa gatgcatgcg ctcccaaacg gtggcttcgc gcgatatcga
     2461 tcttaatctt cagtaatgac gccgaaacga ccgtgctctt catcggcgat tcccgcagtg
     2521 aaaaagactc cttctccgag aggcagaaga tcccataacc cttcgaactc gagcggcgtt
     2581 ccatctgccg tgacaagcgc ttccacaaag gcgccggtaa ccggatcgta attattgatt
     2641 tgcccgtctc caaaattgcc aacccagagc tcgttcccct ccacgacggc aagtccccac
     2701 ggggaattca gatgtctgga tccacatgca ctgccactca gctgtaagta tcggctcgcg
     2761 caaaggcgtt cgtcacaacc gtccctgcta acacaagcag tgcggttttt attgaagcgt
     2821 gaattgtttt cataggttta atgattgttt tttaaaccta aaatcgcagt gcactgtgcc
     2881 ggatagcgca tcagaaaagt aacgccgttg ttacttttgg ggcaccttaa ggatgggccg
     2941 cgttggcgac ctttgttgtc tagtatgacc gtgcagctac atttcttaac gacgaccgtg
     3001 tctggcgttg tctgcgtctt gtgccagctg gatcatgagc gcaaagaacg ccggggattg
     3061 gccgatgaag agccccatct ccgttaggaa ctgcagagtg ccaatggcaa cacctcttgt
     3121 tttagtcggt cgaataatcg aacaaacagg caggcttcga tccaagaacg ggcacggctc
     3181 gggcatgatc agggccttgt cgaagagctc gtcctccacc tccagatccg gaaaagtcac
     3241 cgaaacacca gtcacgggat caacagccgt caaaggaggc gcattacccg ccacatccga
     3301 ccaagtttga aaatgcatgg tctcggtagg cccgatgctg atcaggattc gcaggacttc
     3361 cttccgagtc gccctcagtg ccatcgcggg gtagaggctg ttgccacctt gttcaattgt
     3421 tggaaagtgg aaagccgccg tattcgcaat cgcctgcagg aaattgggat cgcttgtgtc
     3481 ggcatcggtc cggggaattg ccgtgtgcct gttcacccct aaagtcggca cggcctgcgg
     3541 aaaggtgaag tttggatcaa ggtccgggtt atgactgtcg tcgcgataac gcgtccacca
     3601 actggtgtcg atggtaagtt gcgtcaggtt ggtcagtcgt cttccgatta acgccggatt
     3661 gactcccgtc gcggtactgc ccatcagagt gcgaaacgga tcgagattta caggagccgc
     3721 tcccttagag actaggtagg cattgaggaa ctggtggtga gtgatttcat catcagtatt
     3781 gtcatggatg tagtctccca tttcatcctc aataacctcc aaggcctcag tatagtctgg
     3841 attgccactg ccacctggga cttcgtcatc ttggatgccg ccaagttcgt tgtattggat
     3901 ccaaaagtcg gcctccagta tttctgccgc cgctgcgaac cggagaaggg ctgcgtctcc
     3961 gcgcgttaga ccaccacgtg cttccgccgt aggaacattg gctaacaatc ccgcgccgat
     4021 agttcccgct cccacggcaa gcgatttgcc taaaaaggaa cggcgactga taggactttt
     4081 tctctcttcc aagttgtcct ccagaggaag attctggctc tcagtaaaat tattcgctat
     4141 agagttgtgg gttttcatac ccgtagaatc gcagcgattc acactatacg ccgcattgga
     4201 aaagtaacgc ggttgttacc tttggaagcg agccgagcgg cggcgtctcg catggcgaag
     4261 cggccacagc gagtgcgttg accgaatttc aaaagtcagt ggcgagaact cagtgtttgg
     4321 acctactcat ccgtttcgtg ggccaggtga cttttccctg gttcgcgaat tgtgtagccg
     4381 acgtagcgca ccgtatgaat cagcggcgga tcgccgattt tttttcgcaa tcggccgatg
     4441 aaaacttcca ccagcttcgt gtcgtactcg tgctcgtgct cccagacgcg ctcgcacaat
     4501 tcagtgcggg taaagacgcg tcctggttcc cgcatcatca cctttagtag cagcagctcg
     4561 cgtgcggata attcgattcg ctgcgtgccg cgatatacct catgattcgc caaatcgaag
     4621 gtgagatcgc ccactcgcag gttaagcgtc ggctcttccg gataacgtcg cccaagcgcg
     4681 tttacgcgtg ccaccaattc cagcatggca aatggcttgg gcaaataatc atccgcgcca
     4741 agttgcaatc cagtaacgcg atccttgacc tcgccacgag cggtgagcat cagaacacgg
     4801 gtcggcacat gccgtgaatg cagatggcgc aagacctcga agccgtccat ccgaggcaga
     4861 ccaatatcga gcacgatcaa atcgaaccgc gtatccgtcg tttcacgtaa agctctttca
     4921 ccgtcatgga cgataaccgg atcatggcct gcctgtatca gcgccgaggc aatctgccgc
     4981 gtcaattgca cttcatcttc aaccacaagg atgcgcatga gcttgagtca ggtgagatgc
     5041 ctaaaagcgc acggtcaatt gcgctccgag caggtggttg tcattgcggg ggccggtggt
     5101 ttcgtgttga aagctatact ggagcttcaa ctgcgtgtgc gaggtaaaac ggtagcctgc
     5161 cgccacgtcg atccgtgcga gatcctcgct ccagcgaaga tgacctcccg cgccgtcatt
     5221 aatgctgttg aagaattgct ggttccaacg gagcgcaccg aagagttgtg ggctgatttt
     5281 atatttgatc tctaaatagt aagcgaacgt atccacctca cccacgcgcg gaacttcgaa
     5341 gcgcgcctcg taaaattccg cccacaattg cagatgatgc caggcgaagc tgacgtcctg
     5401 ccctagaaca aactctcggt aatcgtcgat accgcgtccg cgcggcaatg tccgctccgc
     5461 ttccggtcga aaatatgcgc cttcgcttgc ggacagacca aagttccatg cctcattcgg
     5521 ccgaaaccca aggcgcgcat tgaaggttgg gttctcgaaa ccgatcgctg tgacgtgcca
     5581 tgattctgga cgcgacgaga gggatgcgtt cttcatttcg gctgcataat caaatcgacc
     5641 tatccggccg gatacagaaa tgccacttgc ataactgggt ccccaaatta ccggattgaa
     5701 ctcgtacttt tcgtagttgt acaacccata gatgaaatcc actggtgaaa acggagcgga
     5761 tttgtcctga attggcgtga cattttcata gattagcggt gcgttgatga atggattttc
     5821 ccacgataaa tgccgtggga cccaattgcc caccacggtt gcaaacgtcc ccacttgcag
     5881 attgaagcgg ccatcatccc acggcgtaaa gcgaagcgca tattcatcga ggcgaacctg
     5941 agctccgcga tcgctggggt cgaacccacg atccagccgc gattgagcaa agaaataaaa
     6001 ctgagatcca agttgtgcat cgagaaacag cgtcagacgc ggattgaaga gagtgtcaat
     6061 gtcgctgttg atgaggccgg gcgcaggctg ttcgaagtga tagatctcca aatcgagcgt
     6121 accgctcaat cgtgtgcgca gattatcctg aaatgcagag accgtgagcg tgctatcgag
     6181 ccgatctaaa aaatcatcga tatcaaacgc gcgtactgtg caggcgaaca gacaaaagct
     6241 cagcgctcgc aataatctca tgtggtttaa gtggcgccgt cggaagtttg cgccgcgatt
     6301 agaaagcgcg cttcgaattc ggtccaatca tcctcggagc gcaccaatcg caaatcgcca
     6361 ttatgcaatc gcgcgagttc ccgcgccaaa ttcaagccga gcccgtgacc ggaaatgtgt
     6421 gaaccagtgc cgccacgatg aaagcgctcg aaaatatgtt cctgcgcgtc gtgtgcgatc
     6481 ggatggccgg tgttgccgac tgttagcaca acttcaccct tgctcgtaag ggcggtaact
     6541 cggataaggc cgcccggccg attgtacttt cgcgcgtttt cgagaaggtt ttgcacgatg
     6601 agcgaggtat atcgtttttc gccggcgaca cataaatcgg ccggaaactg tttctcgatc
     6661 ttcatctcgg gtgaatcggg gagcgcgcca agatcatcaa gccattcctc gatgatctga
     6721 ctgagattga ctggattgga ctcgagttcg agatgacccg cgtccacgcg cgagaggaga
     6781 agcaagtcgt cgattacgcc ggtcaaccgg tgggtgtgat gcaggagcgc ggagagttcc
     6841 tcgtaaattt cagatttgaa accgtcgcgg tgtagtaatc cttcgagtcc caggcgaaga
     6901 acagtcacag gacttttcaa ctgatgggag gcatccgccg agtatcttgt cgatctttcc
     6961 aattcctcag tagtcgaggc gagcgctgct tcggcgcgtt ttcgttccgc gcgattttct
     7021 tccgaatcca gcgccaactt ctcaaccggc cttgcgagcc ggcctgcgat caagtggctg
     7081 gcgacgaatc cgccgagcaa aagcaacacg cccgcgccgc caatttgcca gcggagccga
     7141 ttcaattgag ccatcgaatc agtaagcgga taaatgcaga tttcgtacgc cggcgggaag
     7201 agcgaatccg gattgagccg tttataaaac
//
LOCUS       DASQUQ010000096         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_479779, whole genome shotgun sequence.
ACCESSION   DASQUQ010000096 DASQUQ010000000
VERSION     DASQUQ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479779"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VE486_02600"
     CDS             <1..205
                     /locus_tag="VE486_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZA38009.1"
                     /translation="LPDWAAQIDCKSWAQFFLKWIVANPVVTCAIPATSKPRHMEDNM
                     QACHGRLPDEKMRQRMAELVTTF"
     gene            407..1042
                     /locus_tag="VE486_02605"
     CDS             407..1042
                     /locus_tag="VE486_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZA38010.1"
                     /translation="MKISFRLIHICATATLCAVVTTAPAQTTEKEIAREQAELEKKRA
                     ELERKSAELQQREAEIQRAREELQNQQSGRSLSMNLSGDVLFDYDKATLKPAAEEALK
                     KVAVVLSQFPESMVTVEGYTDSRGTKAFNMQLSQERANAVKDWLVKNGNLPAPNITAR
                     GFGEQNPVAPNTNPNGSDNPAGRALNRRVSIVVEKPASAAQLVTPTATATP"
     gene            1095..1448
                     /locus_tag="VE486_02610"
     CDS             1095..1448
                     /locus_tag="VE486_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38011.1"
                     /translation="MKARLVKFGEIEVEGKRYTHDVVIDGGKVRKRKKGPSKQFREKF
                     GHTPLSAGEEIPWGGKQLIVGTGAHGALPVMEEVLAEAKRRGIEVIAAPTLEVCQLLE
                     EVKKGQAYAILHCTC"
     gene            1503..1745
                     /locus_tag="VE486_02615"
     CDS             1503..1745
                     /locus_tag="VE486_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38012.1"
                     /translation="MFPSRCQFIIVNANHSIMCATKKLRVANCILDCIRLAIPQPTEW
                     QHIGNQINAALIFARADFVNAQGMHDAPASDSRANP"
     gene            1867..2187
                     /locus_tag="VE486_02620"
     CDS             1867..2187
                     /locus_tag="VE486_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38013.1"
                     /translation="MFRHNVYVILLGDAVAKHPSVLRLNPKRDPLKPCVYVGMTGIPV
                     DHRFENHKNGYKSAWVVRKYGVRLIPELYEHLNPMPFEAAVQMEMELAEDLRGAGYTV
                     TGGH"
     gene            complement(2470..3072)
                     /locus_tag="VE486_02625"
     CDS             complement(2470..3072)
                     /locus_tag="VE486_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38014.1"
                     /translation="MQIENLLFILLIAIAVLFKVLASKIGEAKKRSQDPDQSSTTSPP
                     PVEPIRRAPVESDAERIRRFLEALGQPTGSRPPPPVAPRTDIPPRPLAPVQPPPGPIP
                     TARNVLTRRKRQIVEAAKPPAPPVFEPRQALPARAELPPSIEPPLEAYAIDAESKTKV
                     ARTGTDIATLLRSPAGQRNAIILREIFGPPRGLQTRELGT"
     gene            complement(3172..>3711)
                     /locus_tag="VE486_02630"
     CDS             complement(3172..>3711)
                     /locus_tag="VE486_02630"
                     /inference="COORDINATES: protein motif:HMM:NF023549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HZA38015.1"
                     /translation="VKARVTVRSHLDRFVGGATEETIIARVGEGIVSAIGSAHSYKDV
                     LENPDRISKTVLAKGLDSNTAFEILSIDIADVDVGDNIGAKLQTEQADADKRVAQAKA
                     EVRRAAAVAVEQEMRARTQEMRAKVVEAEALVPQAMADAFRSGNLGIMDYVRMKNVQA
                     DTSMRESIAGGGKTRGEGT"
BASE COUNT          840 a         1021 c         1078 g          772 t
ORIGIN      
        1 gttgccggat tgggcggcgc aaattgattg caaatcctgg gcgcaatttt tcctgaaatg
       61 gatcgtggcg aatccggtgg tgacctgcgc gatcccagcc acgagcaagc cgcgccatat
      121 ggaggacaac atgcaggcct gtcacggccg cttgccggat gaaaagatgc ggcagcgaat
      181 ggccgaactt gtaacgacct tttaggtgga ccgcgcgcgc aacgcaaatg gcgcgctcct
      241 cgcatcaaac gacaactggc agagcgacgc aagcgaatcc agacgttcgt ctgattcgtg
      301 cgctaacctt ttcttcgccg ggcgccgata cccccgttgc gcgatcgcct gccagcgcta
      361 tattgttttc cctcaatttc ggggggcgta ctggcttcga caaggaatga aaatatcctt
      421 ccgtctcatc catatttgcg ccacggccac actttgcgcg gtagtgacca ctgctcctgc
      481 gcaaacaacc gagaaagaga tcgcgcggga acaagcagag ttggaaaaga agcgggccga
      541 gctggaacga aaaagtgccg agttacagca gcgagaagcg gagatccagc gtgccaggga
      601 ggagctgcaa aaccagcaga gcggccgctc gctcagcatg aatttgtcgg gcgacgtgct
      661 gttcgattac gacaaagcga cgctcaagcc agcagcagaa gaggcgttga agaaagtggc
      721 cgtcgtgctg tcgcaattcc ccgaaagcat ggtgacagtg gaaggctata ctgattccag
      781 aggcaccaaa gcgttcaaca tgcagctctc gcaggagcgg gcgaacgcgg tgaaagattg
      841 gctggtgaaa aacggcaacc tgcctgctcc taacatcacg gcgaggggtt tcggcgagca
      901 gaacccagtc gcgccaaaca cgaatccgaa tggcagcgat aatccagcgg ggcgtgcgct
      961 gaaccggcgc gtttcaatcg tggtggagaa accggcgtcc gcagcccaac tggtgacgcc
     1021 tacggcgacc gcaacgcctt aagttagaat ccctgcctct tgccttggag ctggagccag
     1081 cggaacattg agatatgaaa gcgcgactgg tcaaatttgg cgagatcgag gtcgagggaa
     1141 agcgctacac gcatgatgtg gtgatcgacg gcggcaaagt gcggaaacgg aagaaagggc
     1201 cttccaaaca attccgcgaa aagttcgggc acacgccgct ttcggccggg gaagaaattc
     1261 cctggggagg caagcagctc attgtcggga ccggcgccca tggcgcgctt ccggtaatgg
     1321 aagaagtcct agcggaagcg aaacggcgcg gtatcgaggt cattgcggcg cccacattgg
     1381 aagtctgtca gctgctggag gaagtgaaga aaggccaggc atacgccatc ctgcattgca
     1441 cgtgttgacc ggggatttgg gagcaaaaag tcccggctct ttgaactcgc gctcctgctc
     1501 gtgtgtttcc atcacgttgc cagttcatca tcgtaaacgc gaatcacagc atcatgtgcg
     1561 cgactaaaaa acttcgcgta gccaattgca ttctggactg catccggctc gccataccac
     1621 agccgaccga atggcagcac atcggaaatc aaatcaacgc cgcgttaatc tttgcgcggg
     1681 cggacttcgt aaacgcgcag ggaatgcatg acgcgccggc cagcgattcg cgggcaaatc
     1741 catgatgaaa tgccagcaag aatcgctgca tgatcatccg ttcggaaaat tgattgaact
     1801 aatctgtccg cattattatt tctctgcggt ttgaaaagga agagaaaaac gcttcagaag
     1861 gaacaaatgt ttcgccataa cgtttacgtt attttgctgg gcgacgcggt cgcaaagcat
     1921 ccttccgttt tgcgcctgaa tcccaaacgt gatccactga agccctgtgt ctatgtgggg
     1981 atgacaggaa tcccagtcga tcaccgattc gaaaatcaca aaaacggcta caaatcggct
     2041 tgggtcgtca ggaaatatgg cgtgcgacta ataccagagc tttacgaaca tctgaacccg
     2101 atgccgtttg aagcagccgt ccagatggaa atggagttgg ctgaagattt gagaggtgcc
     2161 ggatacacag ttacaggcgg acactgacaa acgatcgact cgcgcatgca ggttcaatcg
     2221 acagagcggc tgcggcccgg ctggctgggt gccagcgatc tttatccacc ttcagaaaac
     2281 agtcaccgat gatttttgcg cgggcgcact tcgtaaacgt gctcaagcgc ttcccggaac
     2341 acagacatct tgtctgtgcg gcaaacaggc atcttgcctg ttgggggttg gcggcaacag
     2401 tgggctgaaa gccgcgctgg gggcgcacag gctcgccacg gcgagtccgt cctgcggcgg
     2461 acttcaagcc tatgttccaa gctcgcgcgt ttgtaacccg cgcggcggcc cgaaaatttc
     2521 gcgaaggatg attgcgttgc gttgccctgc cggtgatcgg agcagcgtcg cgatgtccgt
     2581 gccggtgcgc gcgacttttg ttttcgactc agcgtctatg gcgtacgctt ccagcggtgg
     2641 ttcgattgaa ggcggtaact cagctcgtgc tggcagcgct tggcgtggct caaaaaccgg
     2701 cggcgctggg ggtttcgccg cttcgacaat ttgcctcttt ctgcgagtaa gaacattacg
     2761 cgcggtcggg ataggcccag gcggaggttg caccggagcg aggggtcgcg gcggaatgtc
     2821 tgtgcggggc gcaactggag gcggcggtct cgaccctgtc ggttgaccaa gcgcttcgag
     2881 aaatctgcga atacgttcgg catccgattc aaccggcgcg cgcctgatcg gctcaaccgg
     2941 aggagggctg gtcgtcgagc tttgatccgg gtcctgcgat cttttctttg cttcgccgat
     3001 tttgcttgcg agcactttga acaacacagc tatcgcgatc agcagaatga aaaggagatt
     3061 ctcgatttgc atagtgagat cgttaaagag ttaaagcgtt gacaaattga cgcggcagca
     3121 gttgaacact tttaaccctt catcgcttca acgcttcatc gttccaacga atcacgttcc
     3181 ttctccgcgg gtcttccccc cgccggcgat tgattcccgc atggaagtgt ccgcctgaac
     3241 atttttcatt cgcacataat ccattatccc cagattcccg gagcgaaagg catccgccat
     3301 tgcttgcgga acaagcgctt cggcctcgac cacctttgct cgcatttcct gcgttcgggc
     3361 gcgcatctcc tgctctacag ccacggcggc tgcgcggcgc acttcggctt tcgcctgggc
     3421 aacgcgtttg tccgcgtcgg cttgctccgt ctgcaacttt gcgccgatgt tatcgccgac
     3481 atccacgtcg gcgatgtcga tggagagaat ttcaaacgcc gtattgctgt cgagcccttt
     3541 ggccagcacc gtcttggaaa tacgatctgg attttccagc acgtctttat aggaatgggc
     3601 agagccaatt gcactgacaa tgccttcgcc aacacgcgcg atgatcgttt cttcagtcgc
     3661 gccaccgacg aatcggtcga gatgcgaacg cactgtaaca cgcgccttca c
//
LOCUS       DASQUQ010000097         9964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_196876, whole genome shotgun sequence.
ACCESSION   DASQUQ010000097 DASQUQ010000000
VERSION     DASQUQ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196876"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VE486_02635"
     CDS             complement(<1..1026)
                     /locus_tag="VE486_02635"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZA38016.1"
                     /translation="MCKKRESASPKPQPASPSPAAAEAAAGVSPVAAASPAKISKPVV
                     DQTAQTIIFCYHRLVNKVRYPGTEITPADFEAQMKQLKDSGITVIGLQDLLAWKRGEK
                     NIPPRCAVITFDDGWKSQYEVGWPILKKFGYPFTMFIYTEGVRGGGLGGGEAITWEQL
                     ADLRDNGVDIEAHSATHQDLREGHNVMLISSGGKRTKTKLTGPEYEQWVQNEVVGSKQ
                     LLEQRLGIKVNCFAVPFGNYNEHVKELARNAGYEAMFTVYGQPITFTSSLDSLGRYAI
                     EANKPKVFADAVKMIGASTSGASAAAAVGTENLSTQPADGETVRTALPLIKANISSLG
                     AIEPGSVQ"
     gene            1208..1930
                     /locus_tag="VE486_02640"
     CDS             1208..1930
                     /locus_tag="VE486_02640"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZA38017.1"
                     /translation="MPSLISRTLAIAFLASISACTTMHTGRVKDTTKTFTTVVVDAGH
                     GGKDSGTYRRYGPAEKIVALDVAQRLERKLRESQLKTVMTRSSDVFIPLDQRVAIENS
                     QKNAIFVSIHFNDSRKRGIRGFETYYHSGPASELAHRIQSKLMTIPRSGDRGVRTANF
                     RVLRMANYPAVLVECGFLSNRSDGKQARDWEYRELLADRIAEAIVEQRYGVGVYQPTG
                     RLALQLQSASSGPGLAPDTLRQ"
     gene            complement(2097..3329)
                     /locus_tag="VE486_02645"
     CDS             complement(2097..3329)
                     /locus_tag="VE486_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZA38018.1"
                     /translation="MEAIRLFKSLNHVQRNTFVACFLGWALDAFDFFLLTFVLVPVGH
                     EFGRTIPEVAFGITLTLMMRPVGAFVFGLLGDKFGRRIPLMADIIFYSVIELLTAFAP
                     NFTVFLILRALFGIGMGGEWGLGASLAMESLPTATRGLFSGILQQGYAVGYLLAAVVY
                     WIVFPFFGWRGLFVAGALPAFLVIYIRARVPESPVWQRNKERRRHLPRDIRGFIRQHG
                     TLFIHAILLMTAFNYMSHGTQDLYATFLERQRGFGVSEKSMIAIIYAIGAICGGTVIG
                     YFSQRWGRRRAIISCAVCGMLLIPAWIFAPGTALLIVGGFLMQFMVQGAWGVVPVHLN
                     EVSPPDLRGTFPGLAYQLGNFAAAYAAQQQAWLAERFRTAAGTPNYALTMALVECVVF
                     IAIIVLAATGREERGKEF"
     gene            complement(3455..3760)
                     /locus_tag="VE486_02650"
     CDS             complement(3455..3760)
                     /locus_tag="VE486_02650"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HZA38019.1"
                     /translation="MAFEKSKELARSFKRELIVYQRVLRDVRTPVSAKLFLALAIGYL
                     CMPFDLIPDFIPVIGHLDDAIVIPALVLAALRLVPREIVAEYREQVIREEAAISAGR"
     gene            4205..8179
                     /gene="metH"
                     /locus_tag="VE486_02655"
     CDS             4205..8179
                     /gene="metH"
                     /locus_tag="VE486_02655"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZA38020.1"
                     /translation="MSSDLHPLETLLRERIVVLDGAMGTMIQRYKLSETDYRGKRFRD
                     WQGKDLKGSLELLLLTKPGVVEEIHAQYLEAGADIIETNTFSATTIGLHDFLFRGEPA
                     GGRKDQEFFQRVVDDPDLRALVHEMNLVAAKIARRAVERVANGTGTRRFVAGSMGPLP
                     VTASISPDVNDPAFRAVTFDQLKQTYFDQAKALVEGDVDLLIVETIFDTLNAKAALFA
                     IAEVFEETGKKVPLMISGTVTDRSGRTLSGQTVEAFLISIAHAHPLIVGLNCALGPDE
                     MDPFIEEIARISPYFMSAYPNAGLPDPLSETGFPETAKTFTPKVVKWVENGWLNLVGG
                     CCGTTPEHIRAIANAVRDCKPREASGARASALPSSRDHARDNALHLSGLEPVNITPEF
                     GFAVIGERTNITGSPRFSKLILADDFEGALAVARQQVQGGANLLDVNMDEGMIDSEAA
                     MTRFLNLIGSEPEIARIPVVIDSSKWSVIEAGLKCVQGKAVVNSISLKNGEEEFLRQA
                     RLIRRYGAAVIVMAFDERGQADNFERKIEICARAYKLLTEQAGFAASDIIFDPNILTV
                     ATGLEEHRNYAVDFIEATRWIKQNLPGARVSGGVSNISFSFRGNNAVREAMHAAFLFH
                     AIRAGLDMGIVNAGQLAVYEEIEPELQERVEDVLLNRRDDATERLVDFAERVKTKGKT
                     PVQDAAWRAETVEERLKHALVKGITDYIDIDTEEARQKCRRPLEVIEGPLMAGMSVVG
                     DLFGSGKMFLPQVVKSARVMKKAVAYLMPFMEAEKSAGAKPQGRIVMATVKGDVHDIG
                     KNIVGVVLQCNNYEVIDLGVMVPAAKILETAREKKADVIGLSGLITPSLDEMVHVAEE
                     MERERFELPLLIGGATTSRAHTAVKIAPNYHASIVHVLDASRAVGVVNSLLNEQLKSE
                     FDAKTRDDYARLREEHSAKTREKKLLTLEDARANRTPIDWSVYVPPKPEFLGVHVYTS
                     NPGSARVFPGSARGSRAVSGGPPETSNGDQRARGAIESSETSRQAAETNRLAACAPRR
                     FSLRTLIEFIDWSPFFHVWELRGRYPAILDDPVIGKQARELFDDAQELLEQIAAKNLF
                     VARGVYAFWPANSIGDDVDLYVDEECTEKLATFYFLRQQMQKPAGQFNHCLADYVAPS
                     SDGRRPPLQDYLGGFAVSIHGADELAKKFTATHDDYSAILAKALADRLAEAFAEYLHQ
                     QARIAWEFGRDEQLSHVDLVREKYRGIRPAAGYPACPDHAEKRTLFDLLDAERNAGIK
                     LTESFAMHPGASVSGLYFSHLDAKYFGVGQIARDQVVDYAARRGEPTATIEKRLAPNL
                     GYEA"
     gene            complement(8176..9063)
                     /locus_tag="VE486_02660"
     CDS             complement(8176..9063)
                     /locus_tag="VE486_02660"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HZA38021.1"
                     /translation="MFKHEKLNLLIFSVLVALQLLGVIGLLTRFHPFYLFIIIPLFFW
                     GGISTTLYLHRYLTHRGFEMPEWLKFIFATGSAVTLAGDPVTWVGDHRYHHLKSDTDE
                     DIHSPAHGFPYAHMMWLIRKPVQFRERSKRYAADVSKIWYCRLFERSWVYVLPHLFVA
                     ITLYLTLGLAGMLWCLYVPMLVIYNVTWSVNSICHMPRLGYRSFDTSDHSRNNLWIGV
                     LGFGEGYHNNHHAQPRCAAHGLRWWEFDLTRYVIWGLEKCGLAWKVVWPAKEAKDSDA
                     PLTEGATLVLASAKAENPL"
     gene            complement(9395..9468)
                     /locus_tag="VE486_02665"
     tRNA            complement(9395..9468)
                     /locus_tag="VE486_02665"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9432..9434),aa:Pro,seq:tgg)
     gene            9547..>9964
                     /locus_tag="VE486_02670"
     CDS             9547..>9964
                     /locus_tag="VE486_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38022.1"
                     /translation="MTVKGLIFSALLGGITLTLRGQDAAPPAVDLYGTEKQPEVTATP
                     FPEPNGPDLPELSQLDEAFKRRPLSKEVDQRRLHIEWRQLKNQVVNDPEVRAAKAAAQ
                     ATRTDLEKRNRLRDYYNVYYERMSALASSAEMKLALQ"
BASE COUNT         2304 a         2729 c         2869 g         2062 t
ORIGIN      
        1 ttgcacactg ccgggctcga tcgcccccag gctgctgatg ttggctttaa tgagtggaag
       61 cgccgtgcgc acggtttcgc catccgctgg ttgggtcgag agattttccg ttcccaccgc
      121 ggctgccgcg cttgcgccgc tggtagaggc gccgatcatc ttgacggcgt cggcaaatac
      181 tttcggcttg ttcgcttcta tcgcataacg accgagtgaa tcgagcgagg aggtaaaagt
      241 gatgggctga ccgtaaacgg taaacatcgc ttcgtaaccg gcatttcgcg caagttcttt
      301 gacgtgctcg ttataattgc cgaagggcac ggcgaagcaa ttcactttga tgccgagccg
      361 ctgctcaagc agttgtttgg agccaacgac ttcgttttgc acccattgct cgtattcggg
      421 cccggttaat ttcgttttag tcctcttccc gccagaggaa atcagcatta cgttatgccc
      481 ttcgcgcaga tcttgatgcg tcgcggagtg cgcttcgata tcgactccgt tgtcgcggag
      541 gtcggcgagc tgttcccacg tgatcgcctc cccgccgccc aggccacctc cgcgcacgcc
      601 ttccgtgtaa atgaacatcg taaaggggta tccaaacttt ttcaggatcg gccacccgac
      661 ttcgtactga gatttccagc catcgtcgaa tgtaatgacc gcgcatcgcg gcggtatgtt
      721 tttttcgcct cgtttccacg cgagcaaatc ctgcaggccg atcacggtga taccgctatc
      781 cttgagttgt ttcatctgcg cttcgaaatc ggccggcgtg atctcggtgc ccggatagcg
      841 aaccttgtta accagacgat gataacaaaa gatgattgtt tgcgccgtct gatcgactac
      901 tggcttggag atcttcgcgg gtgaagccgc cgcgacaggg ctgacgccag cagccgcttc
      961 cgccgctgcc ggcgatggag aagccggctg gggttttgga gaggcacttt cgcgcttctt
     1021 acacattgaa aggttcagtg ccagaaacag agggagaatc gctaatcgga tcatcataaa
     1081 aagaggtaca ggctttatgt gcggctgccc aaaaaacaag tgctttttcc atgtaaccgc
     1141 cgaatcacgc gatatgaacc cgcaaaaaat ctttggtaga agacgcattt tttgctatta
     1201 agtcagaatg ccgagcctga tcagtcgcac tctcgccatc gctttcctgg cctcaattag
     1261 cgcatgcacg accatgcaca cgggcagagt caaagatacg accaaaacat ttacgaccgt
     1321 ggtagtggac gctggccacg gcggcaaaga cagcggcacg tatcgtcgct acgggccggc
     1381 agagaaaata gttgcgctcg atgtggccca gcgcctggag cgcaagctgc gcgaatcgca
     1441 attgaaaacc gtcatgacgc gatcctctga tgtgtttatt ccactggacc agcgcgtcgc
     1501 cattgagaat tcgcaaaaaa acgccatctt cgtcagcatc catttcaacg attctcggaa
     1561 acgcggcatt cgcggctttg agacctatta tcattccggc cctgcgtcgg aactggccca
     1621 tcggatccag tccaagctga tgaccatccc ccgctccggt gatcgtggcg tccgcacggc
     1681 aaatttccgc gtcctgcgga tggcgaatta cccggcagtg ctggtggagt gcggattctt
     1741 aagtaatcgc tccgatggca agcaggcgcg cgattgggaa tatcgcgaac tactcgcgga
     1801 tagaattgcc gaagcgatcg tcgagcaacg ttacggagtc ggcgtttacc agccaacggg
     1861 ccgactcgca ttgcagttgc agtctgcgag cagcggcccg gggctcgcgc ccgacacttt
     1921 gcgtcagtaa gattcgttgc tgctctgttc gtgccacgca atcatttggg tagcgcacgc
     1981 gtctcgcgtg ttgtttcggt gtcgcgccga aacaatcttt tcttacgccg caattggttt
     2041 cgaaaaacgt tgccctataa aaagttggcg agcgcgagga cgcgtgcgct agccttctaa
     2101 aattctttgc cgcgctcttc gcggccggtt gcggccagca cgatgatggc gatgaaaaca
     2161 acgcattcca ccagcgccat ggtcagcgcg taattcgggg tcccggctgc ggtccggaat
     2221 ctttccgcca gccaggcttg ctgttgcgcg gcgtaggcgg cagcgaaatt tcccagctga
     2281 tatgccagac ccggaaacgt cccgcggagg tcgggtggcg aaacttcgtt caaatgcact
     2341 ggcacaacgc cccacgcgcc ttgcaccatg aattgcatca gaaaccctcc gacgataaga
     2401 agcgctgttc ccggcgcgaa aatccacgcc ggaatcagga gcattccaca cactgcgcaa
     2461 gaaatgatcg cacgccggcg tccccagcgt tgtgagaaat aaccgatgac ggtcccgcca
     2521 cagatcgcgc caatagcgta gataatcgcg atcattgatt tttcactgac gccgaatcca
     2581 cgctgtcttt cgaggaatgt cgcgtaaaga tcctgggtgc cgtgcgacat gtaattgaac
     2641 gccgtcatca gcaggatggc gtggatgaag agcgtgccgt gttgcctgat gaatccgcgg
     2701 atgtcgcgcg gcaagtgccg tctgcgctcc ttgttgcgtt gccaaaccgg cgattccggc
     2761 acgcgtgccc ggatgtaaat gaccaaaaat gcaggcaacg ctccggccac aaacaatccg
     2821 cgccagccaa aaaatgggaa tacgatccag taaacgaccg cggcgaggag ataaccaacg
     2881 gcatagcctt gttgcaaaat tccggagaac agaccgcgtg ttgccgtggg cagtgattcc
     2941 atcgccagcg aagcgcccag tccccattcg ccgcccattc ctattccgaa gagcgcccgc
     3001 aagatcagaa agaccgtaaa atttggggcg aacgcagtga gcagttcaat gacggaataa
     3061 aaaataatgt ctgccatcag cgggatacgc cggccaaatt tgtcacctag cagaccaaag
     3121 acgaacgcgc ccaccgggcg catcattaac gtcagcgtaa ttccaaatgc gacttccgga
     3181 atcgtccgcc caaactcatg ccccaccggc acgagcacga aagtgagcag aaaaaaatcg
     3241 aacgcatcca gcgcccagcc gagaaagcag gcaacgaacg tgttgcgttg aacgtgatta
     3301 agcgacttga aaagccggat cgcttccacc gctttggttt aattgcccat tttacaattg
     3361 ggaagcgcgt tgttgtagcc gaggcgctca gctcgttgcc aggttcgggg ggagcgcacc
     3421 cgcctcagat gctggcgatc gcgcccccgc gaatttacct cccagcgctg atggcagcct
     3481 cttcgcgaat gacctgctcc cgatactctg caacgatttc acgcggcacc aggcgaagcg
     3541 cggcaagaac taatgccggt ataacgatcg catcgtccag gtggccgatt accgggatga
     3601 agtccggaat caaatcgaac ggcatacaca aatagccgat ggccaaagcg agaaataact
     3661 tcgcagaaac cggggtgcga acatcccgca aaactcgctg atacacgatc agttcacgct
     3721 tgaacgagcg agcaagttct ttgctcttct caaaggccat acgatttaat cgggcaacgc
     3781 gaccacttcg tccagtgacc ggaagaatcc gaacgcatat agcgcggcga ggaagtccgg
     3841 ggagcgcagg gccctgccct gagcgaagtc gaacgggctg ccagtctgcg gttgccggca
     3901 gtctctgatt gcggccaata cgcgcggggc gcgattcgat ccgacgcgtc gtctcggcaa
     3961 gctgcctaga cgaacaggct ggtcccgtcg ctgcggatgc tccccaatcc aacgaaacgc
     4021 gcgagcttcg tgctttgtca ttcgtcattc gtcatttgga tcttgtcatt gattgcaaga
     4081 atctgattag gaccaggatt aagagcaaga ataggactag gcccctcaac acatctacgc
     4141 atcatgatgc gtcaatttgt tgcttgatcc ctcggctaaa cgccgtttag ttccccggtc
     4201 aatcatgtcc tctgatcttc atcctctgga aacgctcctg cgcgaacgga tcgtcgtgct
     4261 cgacggcgcc atgggtacaa tgatccagcg ttacaagctt tccgaaacgg attatcgcgg
     4321 gaaacggttt cgcgattggc aggggaaaga tctcaaaggc agcctcgagt tgcttctcct
     4381 caccaagccg ggagtcgttg aggaaattca cgcgcaatac cttgaagccg gcgccgacat
     4441 tatcgaaacg aacactttca gcgccaccac catcggattg cacgattttc tctttcgcgg
     4501 ggaaccagcc ggcggccgca aagatcagga gttttttcaa cgcgtggtgg acgatcccga
     4561 tcttcgggcg ctggtgcacg agatgaatct ggtggcggcc aagattgcgc gccgcgcagt
     4621 agagcgcgtt gcgaatggga ccggaactcg ccggttcgtt gcgggatcga tgggtccgct
     4681 ccccgtaaca gcgtcgattt cgcccgacgt aaacgatccc gctttccgtg cggtcacgtt
     4741 cgatcagctt aaacaaactt atttcgacca ggcaaaggcg ctggtcgaag gagatgtcga
     4801 tcttttgatt gtcgaaacca tcttcgacac gctcaacgcc aaagccgcac tcttcgccat
     4861 tgccgaagtt tttgaagaaa cggggaagaa agtcccgttg atgatttccg gaacagtaac
     4921 cgatcgctcg gggcgaaccc tgagtggtca aaccgtggaa gcgtttctca tttcgatcgc
     4981 gcatgcgcac ccgcttatcg ttggcttgaa ttgcgcgctg ggcccggacg agatggaccc
     5041 gtttatcgag gagatcgcac gcatctcgcc atattttatg agcgcgtacc cgaatgcggg
     5101 tttgcccgat ccgctctcgg aaacggggtt tcccgaaacg gcgaagacgt tcacgcccaa
     5161 agtcgtcaag tgggtagaga acggctggtt gaatctggtg ggcggctgct gtggtacgac
     5221 gcccgaacac attcgcgcaa tcgcaaatgc cgtgcgcgat tgcaaaccgc gcgaagcttc
     5281 gggcgcgcgg gcgtccgcgc tccccagcag ccgcgatcac gctcgcgata acgccctcca
     5341 tctctctggt cttgagccgg tgaatatcac gccggagttc ggcttcgcgg tgattggtga
     5401 acgaacaaat attaccggct cgcccagatt ttcgaaattg atcttggcag acgatttcga
     5461 aggcgcgttg gcagtggccc gtcaacaggt ccagggcggc gcgaaccttc tcgacgtgaa
     5521 tatggacgag ggaatgatcg attccgaggc ggccatgacg cgtttcctga acctgatcgg
     5581 cagcgagccg gaaatcgcgc gcattccggt ggtgatcgac agctcgaagt ggagcgtgat
     5641 tgaagcgggc ctaaaatgcg tgcaaggcaa agcggtcgtc aattccatca gcttgaagaa
     5701 cggtgaggag gaatttctcc gccaggcgcg attgatccgg cgttatggcg cagcggtcat
     5761 cgtgatggcg ttcgatgagc gtggccaggc cgacaacttc gagcggaaga tcgagatctg
     5821 tgcgcgcgcc tacaaattat tgactgagca agccggcttt gccgcaagcg atatcatttt
     5881 tgatcccaat attctgaccg ttgccactgg tcttgaagaa catcgcaatt acgcggtcga
     5941 tttcatcgaa gcaacgcgct ggataaagca gaaccttccc ggagcccggg tgagcggcgg
     6001 cgtgagcaat atttcgtttt cgtttcgcgg caacaacgcg gtgcgcgaag caatgcacgc
     6061 cgcgtttctt ttccacgcca ttcgcgccgg tctcgacatg ggaatcgtca acgccgggca
     6121 gctcgcagtt tacgaagaga tcgagccgga gctccaggag cgcgtggaag atgtgttgtt
     6181 aaaccggcgg gacgacgcga ccgaacggct ggtcgatttc gcggaacgag taaagacaaa
     6241 gggcaaaacg ccggtgcagg atgcagcgtg gcgtgcggag actgtggagg agcgcctgaa
     6301 gcatgcgctg gtaaaaggca tcaccgatta catcgacatc gacacggaag aggcgcgcca
     6361 aaaatgcagg cggccgctcg aagtgatcga gggaccgctc atggccggga tgagtgtggt
     6421 gggagatctg tttggctcag gcaaaatgtt tctgccgcaa gtagtgaaaa gcgctcgcgt
     6481 gatgaagaaa gcggtcgcct atctgatgcc gttcatggag gcggagaaat cagcgggcgc
     6541 aaaaccgcag ggccgcatcg tgatggcgac ggtgaaaggc gacgtccacg atatcggcaa
     6601 gaacatcgtt ggtgtcgtgc tgcaatgtaa caattacgaa gtgatcgatc tgggggtgat
     6661 ggtgccggcc gcgaaaatcc tggaaacggc gcgggagaag aaggcggacg tgatcgggct
     6721 gagcggtctg atcacaccgt ccctggatga aatggtgcat gtggcggagg aaatggagcg
     6781 ggagcggttt gagctgccat tattgatcgg cggcgcaacg accagccgcg cgcacacggc
     6841 ggtaaaaatt gcgcccaatt atcatgccag catcgtgcac gtgctcgatg cctcgcgcgc
     6901 ggtgggcgtg gtcaattcgc tgctgaatga gcaattgaaa tcggagttcg atgcgaaaac
     6961 acgtgatgat tatgcgcgat tgcgagagga acattcggcc aaaacgcgcg agaaaaagtt
     7021 gctcaccctg gaagacgcgc gggcaaatcg aacaccgatt gattggagcg tatacgtccc
     7081 gccgaagccg gagtttttag gagtgcatgt atacacaagt aatccgggta gcgcacgcgt
     7141 atttccgggc agcgcacgcg gctcgcgtgc tgtttcaggc ggcccgccgg aaacttctaa
     7201 tggcgatcaa cgagctcggg gtgcgattga atccagtgag acctctcggc aagctgccga
     7261 gacgaacagg ctggcagcgt gtgctccccg gaggttctcg ttgcgcacgc ttatcgagtt
     7321 catcgattgg tcgccgttct ttcatgtctg ggagctccgt ggacgctacc ctgcgatcct
     7381 ggatgatccg gtgatcggca aacaggcacg cgagcttttt gatgacgcgc aggaactgct
     7441 cgaacagatc gcggcgaaga acttgttcgt cgcgcgcggt gtttacgctt tttggccggc
     7501 gaactcaatt ggtgatgatg tcgatctcta cgtcgatgag gagtgcacgg aaaaacttgc
     7561 cacgttttat tttttgcgtc agcaaatgca aaaacccgcc ggacaattta accattgtct
     7621 cgctgattat gttgccccgt cgagcgacgg tcgcagaccg ccgctacagg attatctcgg
     7681 tggctttgca gtctcgattc acggcgcgga tgaactggcg aaaaagttta cggcgacgca
     7741 tgacgattac agcgcgattt tagcaaaagc cctagccgac aggttagctg aggcgttcgc
     7801 tgaatatctg caccaacagg cccgcatcgc gtgggaattc gggcgcgacg aacaattgtc
     7861 gcacgtcgat cttgtccgcg aaaagtatcg cggtatccgg ccggcggccg gttatccagc
     7921 ctgccctgat cacgcggaaa agcgaacgct attcgatttg ctcgatgcgg agaggaacgc
     7981 ggggatcaaa ctgacggaat catttgcgat gcatcccggc gcaagtgtaa gtggacttta
     8041 cttttcgcat ctggacgcaa aatattttgg cgtcggccaa atcgcccgcg atcaggttgt
     8101 cgattatgcg gcgcgacgag gcgagccgac cgcgacaatc gagaagcgat tggcgccgaa
     8161 tttaggctac gaggcttaga gcgggttctc tgccttggcg ctcgctaata ccagagtggc
     8221 gccttcagtc aaaggagcgt ccgaatcctt ggcttctttc gcaggccaga ccactttcca
     8281 ggcgagacca cacttctcaa gtccccagat cacatagcgc gtgagatcaa actcccacca
     8341 gcgcagtccg tgagcggcgc aacgcggctg ggcatgatga ttattatgat agccctcgcc
     8401 gaacccaagg acgccgatcc aaagattgtt tcggctatga tcggacgtat cgaagctgcg
     8461 atacccgagt ctgggcatat ggcagatcga attgaccgac caagtaacat tatagatcac
     8521 cagcatcggc acgtagaggc accagagcat cccggccaaa ccaagagtta aataaagtgt
     8581 gatcgcgaca aaaagatgag gcagcacgta gacccatgaa cgttcgaaca accgacaata
     8641 ccaaatttta ctgacatcgg cggcgtaacg cttcgagcgc tcacggaatt gaaccggctt
     8701 tcgaatcagc cacatcatgt gggcataggg aaagccgtgc gcgggactgt gaatgtcttc
     8761 gtcggtatcg gatttgagat gatgataacg atggtccccc acccaggtga ccggatcgcc
     8821 ggccagcgtc acagcggatc cggtcgcgaa tatgaacttg agccattctg gcatttcaaa
     8881 gccacgatgg gtcaggtaac gatgcagata gagtgtcgta ctaattccgc cccagaaaaa
     8941 gagcgggatg atgatgaaca gataaaaagg atggaagcgc gtgagaagac caataacgcc
     9001 cagcaattgc aacgcgacca ggacgctgaa aatgagaaga ttgagttttt cgtgcttgaa
     9061 catgcggcgc aggggcaaga agcagccaac ctgggccact caactccaac gcgtcaacac
     9121 tccgatgcaa tgtgtcgatt ggcaaatggt tctttctgga acgggtagcg tctacctttc
     9181 gtgtgttgtt ttcagcacgt cgtaaaaacg gaaattctag ggagtgcacg cgcctcgcgt
     9241 gcacgtttcg gccccctcgc cgaaacgttc ttcttcagaa gcccgaagcg aactcgtttt
     9301 gctcgcagaa gctggcgatc gcgagggcgc gatcgcagca cgcgaggcac ctgcgctccc
     9361 cagaaaccgg gatgcggcat agtctgagtt cacgtcgggg cgagaggatt tgaacctccg
     9421 acctcgtggt cccaaaccac gcgctctacc aagctaagct acgccccgag ttccgacgtg
     9481 attctttatc atggataccg ccttcccgca tccgcaaatt cggttacatt gtggcgaaaa
     9541 cggatgatga cggtaaaagg gttaattttc tcagcgttgc tgggcggcat aacgttgaca
     9601 ttgcgcggac aggatgccgc gccaccggct gtcgatcttt acggaacgga gaagcagccg
     9661 gaggttactg ccacgccttt tccggaaccc aacggaccgg atcttccgga gttgtcgcaa
     9721 cttgacgagg cttttaaacg gaggccgctg agcaaagaag ttgatcaacg ccggctgcac
     9781 atcgaatggc gacaattgaa aaaccaggtg gtgaacgacc cggaagtgcg cgcggcaaag
     9841 gccgccgctc aagctacccg gacagatctg gagaaacgca atcgactgcg cgattactac
     9901 aacgtctatt acgagcgaat gtcggctttg gcttcgagcg ccgaaatgaa gttagcgttg
     9961 caag
//
LOCUS       DASQUQ010000098        11584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_291402, whole genome shotgun sequence.
ACCESSION   DASQUQ010000098 DASQUQ010000000
VERSION     DASQUQ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291402"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /gene="acnA"
                     /locus_tag="VE486_02675"
     CDS             complement(<1..1772)
                     /gene="acnA"
                     /locus_tag="VE486_02675"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZA38023.1"
                     /translation="MKDEFKTLRKFDAGNGREGFLYSLPALEDQGIGTISRLPVSIRI
                     VLESVLRNCDGKKVRRKDVETLANWNAKSPANEEIPFIVARIVLQDFTGVPLVVDLAA
                     MRSAVKRLGGDPKIIEPLVSVDLVVDHSVQVDFFGSARALQLNLEMEFKRNRERYQFL
                     KWGQQAFKTFQLIPPGIGIVHQVNLEYLAKGVLSQKSEVEGQKSEVFYPDTLVGTDSH
                     TTMINGLGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVHMTGQIREGVTATDLVLHIT
                     QLLRAQKVVGKFVEFYGEGAASLPVPDRATIGNMSPEYGATMGYFPIDQESLDYLRAT
                     GRSDEQCRAFENYFRAQKMFGMPRKGEIDYSVDVELDLAEVQPSVAGPKRPQDRISLP
                     ELGKTFRELLEKPVSDGGYGKKHVDLREKHLVELNGSAPRNGNMASSDTKEDQGINPG
                     DELNKIEMVANRPTPDSGREIEAESSEVFARGHSRIGHGSVLIAAITSCTNTSNPSVM
                     IAAGLLAKKAVERGLHVDPAVKTSLAPGSRVVSDYLDKTGLQKYLDELGFNLVGYGCT
                     TCIGNSGPLHPKIEKAIHEFDLVAA"
     gene            complement(1908..2402)
                     /locus_tag="VE486_02680"
     CDS             complement(1908..2402)
                     /locus_tag="VE486_02680"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZA38024.1"
                     /translation="MPLAVGTKAPDFTLSTKTADGPKQIKLSDDFGKKNILLLFFPMA
                     FTGTCTTEMCEVSQGLNAYTNLNAAVYGISGDNPFAQEAWAQKEKITVPLLSDYEHKV
                     AKAYDVMYDSFLPQMNLGMGGVAKRSTFIIDKSGVIQFAESNDDARQLPSFDKIKAKL
                     VELK"
     gene            2559..3047
                     /locus_tag="VE486_02685"
     CDS             2559..3047
                     /locus_tag="VE486_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HZA38025.1"
                     /translation="MELFWWLFTVVLFAVGLIGTVVPVLPGTIIVLAGAIIHRIMLGA
                     EKSIGWKTIVVLVMLTLLSYAFDFLGSYFGAKYFGATRWGAFGAIVGALIGLFFGLIG
                     LFVGPVIGALAGEFIAGKRMIDAGRAGWGSLLGNLGAMIGKLVIALAMIAIFFMTVPP
                     PF"
     gene            3145..3553
                     /gene="rnpB"
                     /locus_tag="VE486_02690"
     ncRNA           3145..3553
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VE486_02690"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3595..3966)
                     /locus_tag="VE486_02695"
     CDS             complement(3595..3966)
                     /locus_tag="VE486_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZA38026.1"
                     /translation="MSIGERDYMRRNDKPPQIRWEYALMVVVGAILLIAFSVQHCSRT
                     TDRHLSKGSLVVNVNEATAKELQTLLGVGAARAELIIQNRPYQSVDELFEKRALPKTV
                     VDANRSRLKTTGKNERTARRD"
     gene            complement(4087..6036)
                     /gene="dxs"
                     /locus_tag="VE486_02700"
     CDS             complement(4087..6036)
                     /gene="dxs"
                     /locus_tag="VE486_02700"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZA38027.1"
                     /translation="MTTKSNSSESSATSEATANTARLLDGIRSPADIKKLHEQDLPQL
                     AQEVRDELIKVLSQTGGHLGPNLGVVELTIALHRVFDTPRDRFVMDVSHQGYVHKMFT
                     GRLDRFSTMRQFGGLNGFLLRTESEHDCYGAGHAGTALSAGLGMAVGRDLRGGDENIV
                     VVAGDAAFTCGISYEALNNVKTHTKRFIVVLNDNEWSIAKNVGAIANYLNSIVTNPTY
                     AHLHDKARDFIQAILGKTGVQLAHKVEESVKGLLVPSVIFEELGLRYYGPIDGHDIPL
                     LIRTFEFLKTQEEPVLLHILTKKGKGYEPAIKQPDKFHGLGKYKIESGETAPTPTPTY
                     SEIIGKTLAKFAEANQKVVTITAAMPSGTGLSHFAKAHPSRYFDVGIAEEHAALFACG
                     LATQGLTPFLTIYSTFFQRAYDMAIHDMAIQKLNVRLCMDRAGLSGDDGPTHHGLFDI
                     GYLRHIPNWVHMQPKNEDEFVDMLWTMAHYNSGPIAVRYPRGPGTGAQIKPEPKLLEI
                     GKAEVVQHGRDVAIFGLGNMFEVAEEAARKLEEKGISVALINPRWIKPMDTGTLEFFA
                     RSVEVVCTIEDHVLYNGFGCAVMEHLHSQLIATPVVRIGWPDQFIEHGAVPILRQKHG
                     ITAEALVEKVLPLLRKKSKLKPSVA"
     gene            complement(6011..6328)
                     /gene="xseB"
                     /locus_tag="VE486_02705"
     CDS             complement(6011..6328)
                     /gene="xseB"
                     /locus_tag="VE486_02705"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZA38028.1"
                     /translation="MSSKSKQREAELNFEGAMDRLEKIVEQMESGKLPLEDLIVRYEE
                     GMNLVKICQERLANAEQKIEIIARNSAGKTVVKDFEPTQEPETPASAIEPQNINDDEI
                     KLF"
     gene            complement(6937..7323)
                     /gene="gcvH"
                     /locus_tag="VE486_02710"
     CDS             complement(6937..7323)
                     /gene="gcvH"
                     /locus_tag="VE486_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZA38029.1"
                     /translation="MNVPDDLLYTESHEWIKREGENVRVGITDHAQSELTDVVYVELP
                     NMDRQANAKEAIAVVESVKAASDIYAPVKGTVVEVNKALEADPGLINREPYGQGWIFV
                     LKIDNADDLKQLNDAEAYRKQIGKGS"
     gene            complement(7320..8420)
                     /gene="gcvT"
                     /locus_tag="VE486_02715"
     CDS             complement(7320..8420)
                     /gene="gcvT"
                     /locus_tag="VE486_02715"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZA38030.1"
                     /translation="MSEAAQKHTPLYDEHVRLGAKIVPFAGWLMPVQYTSILEEHQAV
                     RNNVGIFDISHMGQLIVEGSDAREWLNTMLTNNIDKLDVGTGQYTFLLKEDGGIIDDL
                     IVYRVDDEKFLLVVNASCTDEDFAWLDQHRPKDVDLNNRSADFGGVAIQGPRVTELFH
                     ALFGDDVDLPPRNHIIDLRLDGTTVSAARTGYTGEDGVEVFFRANEAVTFWNAALERG
                     KAFGIKPCGLGARDTLRLEMCYPLNGSDLSPERNPIEAGLGFFVDLTKSNFIGRDALL
                     QTKESGPREKLVSFRMKEKGPPPRPHYAVFENGDRIGGVTSGTLSPSLNWGVGMAYVS
                     ASHAKIGTQIDIEIREKKFPATIEKKPLYKKS"
     gene            8580..9377
                     /locus_tag="VE486_02720"
     CDS             8580..9377
                     /locus_tag="VE486_02720"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZA38031.1"
                     /translation="MTWLDKLERRFGFLGIPGLTRIVVGLTALVWGLVFLNPDFRFAL
                     DLNPARILHGEVWRLITYIFLPQTFSLWVILALWFLWFIGEGLERAWGAFRLTLYFLV
                     GVIGTTAAAFFFGATFSNGMLIVSLFFAFARFYPDEVIYILFVLPMKIKWLAWIMGAF
                     LLFGFVVNGASYRMALVAAFANYLIFFGPEIIHEARHRTEVSARRKRFESDARGEAEP
                     LHKCAFCGATELTDPNLDFRVARDGEEYCMAHLPKAAGVVVDERPSG"
     gene            9592..10413
                     /gene="hemQ"
                     /locus_tag="VE486_02725"
     CDS             9592..10413
                     /gene="hemQ"
                     /locus_tag="VE486_02725"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: protein motif:HMM:NF008913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HZA38032.1"
                     /translation="MMDKNAALVAEEGWHCLHLFYRIEYGQWQLLSREEQNAAKTNLS
                     SLVQEVRAMESTQLLTLSIVTPKADLGFMLITPDLHSANKIEKQLSLSLGPDVMIPVY
                     SYLSLTEESEYITTEEDYAETLTREQNIKPDSEKFAEAMHAFRDRMKHYRQERVYPTL
                     PDWPVVCFYNMSKRRGEQRNWYALPYEERRKLMKGHAAVGREFAGKIKQLITGSTGLD
                     DAEWGVTLFARDTFQIKAIVYKMRFDPVSAEYADFGEFYIGIQLPLDELFRRLQL"
     gene            10428..10515
                     /locus_tag="VE486_02730"
     tRNA            10428..10515
                     /locus_tag="VE486_02730"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10462..10464,aa:Ser,seq:gga)
     gene            complement(10560..11249)
                     /locus_tag="VE486_02735"
     CDS             complement(10560..11249)
                     /locus_tag="VE486_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38033.1"
                     /translation="MFAPADFLDLEHTAHPKLFENQKYVWDALKQIASYLQFRLKPAV
                     LGDLMGKPFISNHVFVGRSTVVEQGAVLKGPAWIGENCQIRSGCYVRENVIVGNGVVM
                     GNSCEFKNCILFDEVQAPHFNYVGDSILGHHAHLGAGVILSNVKLDHGEIAVVTADGN
                     IATGLTKFGAIVGDRTEIGCNAVINPGSVLGRDCMIYPGVNFRGVMPQGSMVKLRQDL
                     QVLEKRDWPAT"
BASE COUNT         2401 a         3225 c         3101 g         2857 t
ORIGIN      
        1 gcggcgacga gatcgaattc gtggatcgcc ttttcgattt tcggatgcag tggtccggag
       61 ttgccaatgc atgtcgtgca accatagcca acgagattaa atccgagctc gtcgagatat
      121 ttttgcaaac cggttttgtc cagataatcg gaaacaacgc gcgagccggg tgcgagcgaa
      181 gttttcaccg caggatcgac atgcagcccg cgctcgaccg ctttttttgc aagcaaaccg
      241 gcagcgatca tcacactcgg attgctcgta ttggtgcagc tcgtgatcgc tgcgatcagt
      301 acgctgccat gaccaatccg actatgcccg cgtgcgaaca cttcgctgga ttcggcttca
      361 atttctctcc cagaatcggg cgtcggccgg ttcgcgacca tctcgatctt attgagctcg
      421 tcgcccgggt taattccctg atcttctttt gtatcggacg acgccatgtt gccattgcgt
      481 ggcgcgctgc cattcagttc cacgagatgt ttctcgcgaa gatcgacatg ctttttgccg
      541 tagccgccat cgctcaccgg tttttcgagc aactcgcgaa atgtcttccc aagttccggc
      601 aaactgatcc ggtcctgcgg cctctttggt ccagcgacgc tgggttgcac ctctgccagg
      661 tcaagttcaa catcgacgct gtagtcgatt tcgcctttcc gcggcatccc aaacattttt
      721 tgtgcgcgaa aataattttc gaacgcgcgg cattgctcgt cgctgcgtcc cgtagcacgc
      781 aaataatcca gcgactcctg atcaatcgga aaatagccca tggtcgcgcc gtactcgggc
      841 gacatgtttc caatcgtcgc gcggtcggga accggcagcg atgctgcgcc ttccccataa
      901 aactcgacaa acttaccgac cactttttgc gcgcgcagca attgggtgat gtgcaagaca
      961 agatccgtcg ccgttacacc ttcgcgtatc tgaccagtca tgtgcacgcc caccacgtcc
     1021 ggcgttagaa aataaaccgg ctgcccgagc atgccggcct ccgcttcgat cccgccgacg
     1081 ccccagccca ccacgccaag accgttaatc atcgtcgtgt gcgagtccgt tcccacgagc
     1141 gtgtccggat agaagacttc tgacttctga ccttcgacct ctgacttctg actcaaaacg
     1201 cctttcgcga gatattcgag gttcacctgg tgaacgatgc cgattccggg cggaatgagc
     1261 tggaaagtct tgaacgcctg ctgtccccat ttcaaaaact gataacgctc ccgattgcgc
     1321 ttgaactcca tctccaaatt gagttgaagc gctcgcgctg aaccgaagaa atccacttgc
     1381 accgagtgat cgaccaccag atcgacagac acgagcggct caatgatttt cggatcgcca
     1441 ccgagccgtt tcaccgcgct gcgcatcgcc gcaagatcga ctacgagcgg cacgccggtg
     1501 aaatcctgca atacgatgcg tgcgacgatg aaaggaatct cttcattcgc cggtgatttt
     1561 gcattccaat tcgccagtgt ctcgacatcc ttgcgccgga cctttttgcc gtcgcaattg
     1621 cgcaaaaccg attcgagcac gatgcgaatg cttactggca gccgcgaaat tgtgccgatc
     1681 ccttggtctt ccaaagcagg cagcgaatag agaaagcctt ctcggccatt gcccgcgtcg
     1741 aatttccgca gcgttttaaa ttcgtccttc attttcaatt cggttatcct gagcgaagcg
     1801 aaggacctca cacaaactcg aaacggcaca catagtaatt tgagtgatcg aaagcacgat
     1861 tgtgggtccc tcgccgtctg ctcgggatga cagcgcggtg tgcgggttca tttaagctcc
     1921 accagcttgg ctttaatctt gtcaaagcta ggcagctgtc tcgcgtcgtc gttgctctcg
     1981 gcaaattgga tcaccccgct cttatcgatg atgaaagtgg atcgtttggc gacgcctccc
     2041 attccgagat tcatttgcgg cagaaacgag tcgtacatca cgtcataagc ttttgcgact
     2101 ttgtgttcgt agtcgctcaa aagcggcacg gtgatcttct ccttttgcgc ccacgcttcc
     2161 tgagcaaacg gattgtcgcc gctgattccg taaacggcgg cgttcaagtt cgtgtaagcg
     2221 ttcaggccct gactgacttc acacatttca gtcgtgcatg tgcctgtgaa cgccattggg
     2281 aaaaagagaa gcagaatgtt tttcttcccg aaatcatcgc tcagcttaat ctgctttggc
     2341 ccgtcggcgg tcttggtcga taacgtgaaa tctggggctt tggttccaac tgctagcggc
     2401 ataatggatt ctccggtttc gagttaaatg tgacacttcc agtataggat cgacatctag
     2461 ccggtcaaag agagaacgtg attcgttaaa ccgttagatc gtgaatcgtc atatcggcat
     2521 ttgccttccg attcactaat cacgattcac gttttcccat ggaattattc tggtggcttt
     2581 tcacggttgt gctgttcgcc gtcggcctga tcggcacggt cgttccagtt cttccgggca
     2641 cgatcatcgt tctggccggt gcaatcattc accgcatcat gctcggcgcc gagaagagca
     2701 tcggttggaa aacgattgtc gttctcgtaa tgctcacact cctttcctac gcgttcgatt
     2761 ttctcggcag ttatttcggc gcgaaatatt tcggtgcgac aagatggggc gctttcggcg
     2821 cgatcgtcgg cgccctcatc gggctcttct tcggtctcat cggtttgttc gtgggcccgg
     2881 tgatcggtgc cttagcgggc gaattcatcg ccggcaaaag aatgatcgac gccggccggg
     2941 caggctgggg gagcttgttg gggaatctcg gcgcaatgat cggcaaacta gttatcgcgc
     3001 tcgccatgat tgcgattttc ttcatgaccg tgccgccgcc gttttgagaa tgaaattgta
     3061 gggcaaccgc tccggttgcc gaaaccagct ggcatgcggt gcgcgtgctc tacaattgct
     3121 tccgccgatt aaggggctag attagcgcga tggcagatga tcactggtaa gccactttgg
     3181 cttgctagag gaaagtccga acaccatacg gcagcatgcc gcgtaaaaaa cgcgggcgcc
     3241 gcagttgcaa aattgcggtg acggagagtg tcacagaaaa gacaccgccg gttctttggc
     3301 aacaaaggca caggcaaggg tgaaaaggcg aggtaagagc tcaccgctcg cggagtaatt
     3361 cgcgaggcag gaaaaacccc ctgtggtgca agacaaaaca gagggagggc agccggtccg
     3421 tttaatcctc gggtatcgtc gcatcgcgat gcgcgcaagc gcatcgggcg ctcggagaaa
     3481 ttcgagtgag acaaatgatc atccgcgtga gcaatcacgc tgacagaatt cggcttatcg
     3541 ccatcaaaca tatgaagggc gctttcggaa acgaaggcgc ccttcgcttt ttgatcaatc
     3601 ccggcgcgcg gttcgctcgt tcttgccggt tgtcttcaac cgggagcggt ttgcatcaac
     3661 gacggttttt ggcaacgcgc gcttttcgaa aagctcatcg acggattggt atggccgatt
     3721 ctgaataatg agttcagcgc gtgcggcacc aacgcccagc aacgtctgca attccttagc
     3781 ggtcgcctcg ttaacattta cgaccagaga gcctttgcta aggtgacgat ctgtcgtcct
     3841 tgaacaatgt tgtacactga atgcgatcag gaggattgcg ccgacgacaa ccattaacgc
     3901 atactcccac ctgatttgcg gcggtttgtc gtttcgccgc atgtaatcac gttcgccaat
     3961 gctcacggtt cactccgatt gctgcgaatc ggcaagctaa cggttttttc ttgcctgatt
     4021 aaacggccat ctatggcagc tggcggcgac tgtttcccgc cctccttgtc tattaacacc
     4081 gctaattcag gctacagatg gcttgagctt cgattttttg cgcagaagcg gcagcacttt
     4141 ttccacaagc gcttcggcgg tgataccgtg tttctggcgc aggattggaa ctgcgccgtg
     4201 ctcgatgaat tgatcgggcc agccgatgcg cacgaccggc gtggcaatca attgcgaatg
     4261 cagatgttcc atcaccgcgc agccaaaacc gttgtacaga acatgatctt cgatcgtgca
     4321 gacgacttcg acgctgcggg cgaagaattc gagagtgccg gtgtccatcg gcttgatcca
     4381 gcgcggattg atcagcgcaa cggaaattcc tttctcttca agtttccgcg cggcttcttc
     4441 cgcaacctca aacatgttgc ctaatccaaa gatcgccacg tcgcggccgt gttgcacgac
     4501 ctccgccttg ccgatctcga gaagctttgg ttctggtttg atttgcgcgc ctgtgcccgg
     4561 acctcgcggg taacgaacgg cgatcggacc ggaattgtag tgcgccattg tccagagcat
     4621 atcgacaaat tcgtcctcgt tcttcggctg catgtgcacc caattcggga tgtggcgcag
     4681 ataaccgatg tcgaacaaac catgatgtgt cgggccatcg tcgccgctta acccagcgcg
     4741 atccatgcag agtctgacgt ttagtttttg gatcgccatg tcgtgaatcg ccatgtcgta
     4801 ggcgcgctga aaaaacgtgg aatagatcgt gagaaaggga gtcagacctt gcgtcgccaa
     4861 cccacaggcg aaaagcgcgg cgtgctcttc cgcaatgccg acgtcgaagt agcgactggg
     4921 atgcgccttg gcgaaatgcg aaaggcccgt tccgctcggc atggcggcgg tgatggtgac
     4981 gaccttctga ttggcttccg cgaatttcgc cagtgtcttg ccaatgatct cggaataggt
     5041 cggagtcggc gtgggagcag tctcgccgct ctcgatcttg tatttgccga gaccgtgaaa
     5101 tttgtccggc tgtttaatag ccggttcgta ccctttgcct ttcttggtga gaatgtgcag
     5161 gagcaccggt tcctcctggg ttttcaaaaa ctcgaatgtc cggatcaata acgggatatc
     5221 gtgtccatcg atcgggccgt aatagcgcag gccgagctct tcaaaaatca cgctcggcac
     5281 gagcaaacct ttgacgcttt cttccacttt gtgtgcaagc tgcacgcccg tttttccaag
     5341 aatcgcctgg atgaaatcgc gcgccttatc gtgcagatgc gcgtaagtgg gattcgtcac
     5401 gatgctattc agataattag caatcgcgcc aacgtttttt gcgatcgacc actcattgtc
     5461 gttgagcaca acgatgaagc gcttggtatg cgttttgaca ttgttgagcg cttcgtagct
     5521 gattccgcag gtgaatgcag catcgcccgc caccactacg atattctcat cgcccccgcg
     5581 caaatcgcgt cccacagcca tgccgagacc agccgaaagc gccgtgccgg catgaccggc
     5641 gccgtagcaa tcgtgttcgc tctccgtgcg taaaagaaat ccattgagac cgccaaattg
     5701 ccgcatcgtg gaaaaacgat cgagccgccc tgtaaacatc ttgtggacat agccctggtg
     5761 actgacgtcc atgacaaagc gatcgcgcgg cgtatcgaac acgcgatgca gcgcaatggt
     5821 caattcgacc acgccgagat ttggacctag gtgtcccccg gtttgggaaa gaactttgat
     5881 cagctcgtcg cgtacttcct gtgcgagctg gggaaggtcc tgttcgtgca gttttttgat
     5941 gtccgcgggc gaacggatcc cgtcgagtaa tcgcgcggtg ttggccgtcg cttcagacgt
     6001 tgcggaggat tcagaagagt ttgatttcgt cgtcattgat gttttggggt tcgatcgcac
     6061 tggccggcgt ttccggctcc tgggttggct cgaaatcttt aactacggtt ttgcccgcgc
     6121 tgttgcgggc aatgatctcg atcttttgct ccgcgttggc aagccgttcc tggcaaattt
     6181 tgaccagatt cattccttct tcatagcgca caatcaggtc ttccagaggc agtttgcccg
     6241 actccatttg ctcgacaatt ttttcgaggc gatccattgc gccctcaaag ttgagctctg
     6301 cttcgcgctg cttagatttg ctgctcacgg ggaggaggtg gaattaagca caggttggtc
     6361 aagaggaaag cgcgacttgt tcagtctttc gaccgctgta gatggtactt atctagcaaa
     6421 gaatcataca atttattcgg atctgaagtt gtcgtaattt cccgagggcc gttaaaattg
     6481 tatgtgtgta tccgaaagca ctcgaagcag atcgatacgt cgaattgcct tgagttcgtc
     6541 atcacgcgta atccgtgtcg tggattaaag cataacgcaa ccgtcccgtc gctcttctca
     6601 attcccttca cgagcgaatc aattaatgcg accctatcag tcgcgttttt gacctcaact
     6661 tcgcctagta ttggaaaccc atggaagact gttcctgtgt tcgtcgcata acccttggga
     6721 ttatttggat ccaaggaata gagaaagatg cgcgcttttt cattagtgat ggttcgcaag
     6781 gctgctgcgg tctgcttgtt cctctccaag agtctttgct gcgacttctc aaaccacttg
     6841 ttcgccggtg actcggttgc ggtttgaccg tttaacagtg ctacgtcaat caagaacatc
     6901 gttatccaca ggacaacgag aaggcgtttc atctttttaa gatcccttcc cgatctgctt
     6961 ccgatacgcc tcagcgtcat tgagttgttt aagatcgtcg gcgttgtcga tcttgaggac
     7021 gaaaatccag ccctggccgt agggttcgcg gttgattaag ccggggtccg cttcgagcgc
     7081 tttgtttact tcgacgactg ttcccttgac cggtgcgtaa atgtcgctcg cggctttcac
     7141 cgattcgacg accgcgattg cttccttcgc gttcgcttgc cgatccatgt tcggcagctc
     7201 gacgtaaact acgtcggtca attcagattg cgcgtgatcg gtgatcccga cacgaacatt
     7261 ctcgccttcg cgtttaatcc attcgtggct ctcggtgtaa agcaaatcgt cggggacatt
     7321 catgattttt tgtagagtgg ctttttttcg atggtcgcgg gaaatttctt ttctctaatt
     7381 tcgatgtcga tctgggttcc gatcttcgcg tgcgaggccg agacatatgc catcccgacg
     7441 ccccagttca agctcggaga aagtgtgcca ctggtcactc ccccaatgcg atcaccgttc
     7501 tcgaagaccg cgtagtgcgg ccgcggcgga gggcctttct ctttcatccg gaacgaaacg
     7561 agcttttctc gcggaccgct ttctttcgtt tgcagcaaag cgtcccgccc aatgaaattc
     7621 gatttggtaa gatcgacaaa aaatccgagt ccggcctcga taggatttcg ttccggggac
     7681 agatcggagc cgttgagcgg ataacacatt tccaagcgca atgtgtcgcg cgcgcccagt
     7741 ccgcacggtt taattccgaa ggctttgcct ctttcgagcg ccgcattcca aaacgtcact
     7801 gcctcgttcg cgcgaaagaa aacttcaacg ccgtcctctc cggtataacc ggtccgtgcg
     7861 gctgaaacag ttgttccgtc caagcgaaga tcgataatat gatttcgtgg cggtagatcg
     7921 acatcatcgc cgaagagcgc atgaaataat tccgtgacgc gtggcccttg aatggcgact
     7981 ccgccaaaat ccgcgctgcg attatttaga tcgacatcct ttggtcgatg ttggtcaagc
     8041 caggcaaaat cctcgtccgt acacgatgcg ttcacgacga gcagaaattt ttcatcgtcg
     8101 acacgataaa cgatcagatc gtcgatgatg ccgccgtctt ctttcagcag aaaggtatat
     8161 tgacccgtgc cgacgtcgag cttgtcgatg ttatttgtga gcatggtgtt gagccactcg
     8221 cgcgcgtcgc ttccttcgac gattaattgg cccatgtgcg agatgtcgaa aatgccaacg
     8281 ttatttcgca cagcctgatg ctcctccagg atacttgtgt attggaccgg catcagccac
     8341 ccagcgaacg gcacaatttt cgcgccaagc cgcacgtgtt cgtcgtaaag tggagtgtgt
     8401 ttctgtgcgg cttcgctcac gcgcagacct tagcacaggt aggggcgatt gacctcaatc
     8461 gccctgcata cgtgttaacg aaacgtgtgg gtcgcttgcc agacacagag ccaggcggtt
     8521 cgggtgaacc gcccctacct tgaaaacaaa acgcgtgccc cttacgctcc cgcaactaaa
     8581 tgacctggct ggataaactc gaacgccgtt ttggttttct cggtattccc ggactgaccc
     8641 gcatcgttgt cggcctcacg gcgctcgttt gggggctggt cttcctgaat ccggatttcc
     8701 gttttgcgct cgatctcaat ccggcgcgta tcctccatgg cgaagtctgg cggctgataa
     8761 cttacatttt tctcccgcag accttcagtc tttgggtcat ccttgccctc tggtttcttt
     8821 ggtttattgg cgaaggcctc gagcgtgcgt ggggcgcgtt tcggcttacc ctctattttc
     8881 ttgttggcgt gatcggcacg acggcggccg catttttctt cggcgcaaca ttttcgaacg
     8941 gcatgctgat cgtgtcgttg ttttttgcct tcgcccggtt ttatcccgac gaagtcattt
     9001 acatcctttt cgttctgccg atgaagatca agtggctggc gtggatcatg ggcgcgtttt
     9061 tgctttttgg gttcgtcgtc aatggggcct cgtatcgcat ggcgctggtg gccgcgtttg
     9121 ccaattatct gatcttcttc ggcccggaaa ttattcacga agcacgccac cgcacggaag
     9181 tctcggcccg gcgcaagcgt tttgaatcgg acgcacgggg cgaagccgag ccacttcaca
     9241 aatgcgcatt ctgcggcgcg accgagttga ccgatccgaa tctggacttc cgtgtcgcgc
     9301 gcgacggcga ggaatattgc atggcgcatt tgccgaaagc ggccggagtc gtcgttgacg
     9361 agcgcccgtc tgggtagcgc acgcgtctcg cgtcgtttgg ggagcacagg ctgccagcct
     9421 gtagttgccg gcagccctgc cggcaacatc tgtgcacgcg cgattccgag ggacgccaat
     9481 tcccggtctc ggcaagctgc cgagacctgc gggctggcag cctgcgctcc ccggcacgca
     9541 tgccactcgc gctacccttg accgcgactc cgcacggatg agactagcac gatgatggac
     9601 aagaacgcgg cgctcgtcgc cgaagaaggc tggcattgtc tgcatctgtt ttaccggatc
     9661 gagtacggac aatggcagtt gctcagtcgg gaagaacaaa atgccgccaa gaccaatctc
     9721 tcttcgctcg tgcaggaagt gcgcgcgatg gaatcgaccc agctgttaac ccttagcatc
     9781 gtgacgccga aagcggacct cgggttcatg ctgatcacgc cggatttgca cagcgcgaat
     9841 aaaatcgaga agcaactctc cctctcgttg ggccccgacg taatgatacc ggtttacagt
     9901 tatctctcgc tcactgagga aagtgaatac atcacgacgg aagaagatta cgcggagacg
     9961 ctgaccagag aacaaaacat caagccggac tcggagaaat tcgccgaagc gatgcatgcg
    10021 tttcgcgacc gaatgaaaca ttaccggcag gagcgggttt acccgacgtt gcccgattgg
    10081 ccggtggttt gcttctataa tatgagcaaa cgccgcgggg agcagcgcaa ttggtacgcg
    10141 cttccctatg aagagcgccg caaattgatg aaaggacacg ccgcggtcgg ccgtgaattc
    10201 gccgggaaaa taaaacagct catcactggt tcgaccggtc tcgacgatgc cgagtggggc
    10261 gtgacactct ttgcgcggga cacatttcag atcaaagcga tcgtttacaa aatgcgtttc
    10321 gatccggtga gcgcggagta cgcggacttc ggcgaatttt acatcggcat tcaattgccg
    10381 ctcgacgaat tgttccggcg attgcagctt tgattgttgc gcttacagga tgggtgacag
    10441 agcggtctat tgtgcacgcc tggaaagcgt gtgtgccgaa aggcaccggg ggttcgaatc
    10501 cccccccatc cgccacttct cttcttctta ctctttactc ctgacgctgc tagccccgtt
    10561 taagttgcgg gccagtcgcg tttctccagg acctgaagat cctggcgcaa tttaaccatg
    10621 ctaccctgcg gcattacgcc gcgaaaattt acgcccggat aaatcataca atcgcgcccc
    10681 aggaccgagc ccgggttaat gacggcgttg catccaatct ccgtccggtc gccgacgatc
    10741 gcgccgaatt ttgtcagacc agttgcgata ttgccatcgg cggtcacgac cgcgatttcg
    10801 ccgtgatcga gtttcacgtt cgagagaatc acgcctgcgc caagatgagc gtggtgaccg
    10861 aggatggaat cgccgacgta attgaaatgc ggcgcctgca cttcgtcgaa cagaatgcaa
    10921 ttcttgaatt cgcatgagtt tcccatgacc acgccattcc cgacgatcac gttctcgcgc
    10981 acgtaacagc cgcttcggat ctggcaattt tctccgatcc atgccggccc tttcagcacg
    11041 gcgccttgct ccacgacggt gcttctgcca acaaaaacgt gattgctgat gaatggtttg
    11101 cccatcaggt cgcccaaaac cgcgggcttc aagcgaaatt gcaggtaact cgcgatctgt
    11161 ttgagcgcgt cccagacgta cttctgattt tcgaacagct tcggatgagc cgtgtgttcg
    11221 aggtcaagaa aatcggcagg cgcaaacatt ttcagcggtc aagatgacga atcgctccgc
    11281 gacttgcgag cggaaattca ggggagtgcg cgccgccggc ttgctccgtc ccggccgctg
    11341 gccggacggt tccagaggca ccgcggtttc gctgttgatt gttccacgcg atacaggtca
    11401 tcagcatttt gggaggtttt cggccagcgg ccgaaaacgg cacgccagcg gcgtgcgctc
    11461 cctagattac gctgcgccgc gcggcgccag tttttggcca tcttttgtgt cgcgcacttg
    11521 ccagccgagg gcggaaatct cctcgcgtaa ttcatcgctc tgtttccaat ttttttcgct
    11581 cctg
//
LOCUS       DASQUQ010000099         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_481070, whole genome shotgun sequence.
ACCESSION   DASQUQ010000099 DASQUQ010000000
VERSION     DASQUQ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481070"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VE486_02740"
     CDS             <1..387
                     /locus_tag="VE486_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38034.1"
                     /translation="SGRFSINQRHHANRFVFLTEIFLAFIAVSLIFQTESLRGKTPPS
                     RRFKAGAIRGFLAHGCIRRIHVAHVIIDGVIDKPGVRKTRKPSDKSPLLEPEAIADSY
                     WQLIEQDRSAWSLELDLRPHNEAFFE"
     gene            complement(426..1043)
                     /locus_tag="VE486_02745"
     CDS             complement(426..1043)
                     /locus_tag="VE486_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZA38035.1"
                     /translation="MWRERLLESTRIRVLALLQKKDRTVNELADDLRLTDNAVRAHLT
                     SLERDGLVRRTGREKGVRRPHVTYGITAEAEHIFARAYGLLLNHFVAVAARRLGPRAL
                     RACMRQVGRRIASEQPAKLQNQSKTQRINATLALLKEMGGAASFSVRERKHFIVGNGC
                     PFTAVTTDHPEACLIAESLLSEIIGVPVKEHCIHAPTPSCRFEIS"
     gene            1168..1563
                     /locus_tag="VE486_02750"
     CDS             1168..1563
                     /locus_tag="VE486_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38036.1"
                     /translation="MANSTDTPSARNQYANQAFQILRFGFTVAPILAGLDKFFHLLVD
                     WDKYLSPMANNVLAGHGHEFMLLVGVIEIVAGIGVFLKPRIFAYIVAAWLLLIILNLL
                     SIPGYYDIALRDLGLMLGALALGRLSQNR"
     gene            complement(1810..2982)
                     /locus_tag="VE486_02755"
     CDS             complement(1810..2982)
                     /locus_tag="VE486_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38037.1"
                     /translation="MNRKSYARHALAAFCAAALTLTAPAFAGFNADAPNAIRRIFTQL
                     ALDDGRLVSYHVLEPGPAGVEAFPTGSSTGKLFRFPSCPNLRPVLDDSAQPSHTNQIV
                     LDNAMREVFNVTLSGCDIQPTSASDVLRTARSMQSIGFVNAPSVPAPIGAPVPSNEQQ
                     SDDELWGPVPNIGVVDVFAGGNLSPQHIQGTTLSGLPIANVQQPDIRRPRISAYAAGQ
                     VVYFITYETKNLSRSGLVPRAVENQWSGSGFPGERDLFFCAYGRAPLPPNAALPAGSL
                     DSNGVPNDNQAVLNIVKGAPFRNVGDYSPLWKMHCVNGDISPAIGPGFPCGSTRFYQI
                     GQPHSLDELTATGIRIEGGIFRDINCPILATDVNDDGNFANTRASKEVVQFADVAW"
BASE COUNT          718 a          959 c          906 g          768 t
ORIGIN      
        1 agcggccggt tttccatcaa ccagcgacat cacgcgaatc gcttcgtttt tctcaccgag
       61 atatttctgg ccttcatcgc cgtctccctg atttttcaga cggaatccct taggggaaag
      121 acgccgccct ccaggcggtt caaagccgga gcaattcgag ggttcctggc gcacggctgc
      181 attcggcgca tccatgttgc gcacgtcatc atcgatggcg tgatcgacaa accaggggtg
      241 cgcaaaacgc ggaagccctc cgataaaagt cccctcctcg agcctgaagc gatcgccgat
      301 tcatactggc aacttatcga acaagaccgc agcgcatgga gtttggaact tgatttgcga
      361 ccgcataatg aagcgttttt cgagtgatct gcaggccctt tcagactgcg ataccacgtg
      421 gcgtctcaag aaatctcgaa tctgcaagac ggcgtcggag catggatgca gtgctccttt
      481 accgggacac cgatgatctc actgagcaac gactcggcaa tcaagcatgc ttccgggtga
      541 tccgtggtca ccgcagtgaa tgggcagcca ttcccgacga tgaagtgttt tctttctctc
      601 acagaaaagg atgccgcgcc ccccatttct ttaagcagag cgagagtcgc gttaattctt
      661 tgggtcttgc tttggttctg gagtttcgct ggttgttcgc tggcaattct ccttccgact
      721 tgacgcatgc aggcgcggag agcgcgggga ccgagccgcc gagccgcgac ggccacgaaa
      781 tggttcagga gtagcccata agctcttgca aaaatgtgtt ctgcctccgc ggtgataccg
      841 taggtaacgt gcggacgtcg cacgcccttt tccctgcctg ttcggcggac aagaccatct
      901 cgttcgaggc tggtcaggtg cgcccggacg gcgttgtcgg tcaaccgcag atcatccgcg
      961 agttcgttga ccgtccggtc tttcttctgg agcaaggcca gaacgcggat gcgcgtgctc
     1021 tcgagcagtc gttcccgcca catagaggtc ttcacgtcac agagtaaccc tcgcgttaaa
     1081 attagtcaaa ataatcattg actatttatg gatcagcttc aggatttcga atcgttcgaa
     1141 accctcaact ccaacccaac aaatcctatg gcaaattcaa ccgacacacc ttccgctcga
     1201 aatcaatatg ccaaccaggc gttccagatt ctgcgttttg gatttaccgt ggcgcccatc
     1261 ctggccggcc tcgataaatt ctttcatctc ctcgttgact gggacaaata cctgtccccc
     1321 atggcaaata atgtccttgc cggccacggc catgaattca tgctccttgt cggagttatc
     1381 gaaatcgtcg ccggaattgg cgtctttctg aagccgcgga ttttcgctta cattgtcgcg
     1441 gcttggctac tgcttattat tcttaatctt ctaagcattc ccggttacta cgacatcgcg
     1501 ctgcgcgatc tcggcctgat gctgggggct ctggctctcg gccgattaag ccaaaataga
     1561 taacgtcggc cgtcaacgcg cccgcggatc ccctcctcca gcccgaaacg atcgctgatt
     1621 tctactggcg tctcatcagg cagcagcgca tggagtttgg aaatgcaccc gccctgataa
     1681 tgagacgttc ttcgaatgat tgcttccatt gcgcgagcgg aaagcaaaga ttaactaatt
     1741 agcgcgatcg cagaaaaagc ttacttcagc gtcgagcttg aatctgcgat tccactgtca
     1801 ggctggccat taccaggcta catcggcgaa ttgcactact tcctttgagg cacgtgtgtt
     1861 cgcgaaattg ccatcgtcat tgacgtcagt cgcaagaatc ggacaattga tgtcgcggaa
     1921 aatcccgccc tcgatccgaa tcccagtggc cgtgagttca tccagactgt gcggctgacc
     1981 gatctggtaa aaacgggtgc tgccacaagg gaacccgggt ccgatagccg gcgaaatatc
     2041 gccattgacg cagtgcatct tccagagcgg gctgtaatcg cccacatttc ggaaaggcgc
     2101 gcctttgaca atgttgagca ccgcctggtt atcgttaggc accccattgc tgtccagcga
     2161 gccagcgggc aatgcggcat taggtggcag cggtgcccgg ccataagcgc agaaaaacaa
     2221 atcgcgctct ccaggaaaac cactgccgct ccactgattt tcgacggctc tggggacgag
     2281 tccgctgcgc gaaagatttt tcgtttcgta ggtaatgaaa taaacaacct gccccgcggc
     2341 gtaggcgcta atgcgggggc ggcggatatc aggctgctgc acgtttgcga tgggcaaacc
     2401 actgagcgtt gttccctgga tgtgctgcgg cgaaaggttg ccgccggcga aaacatcgac
     2461 aacgccgata ttcggcaccg ggccccacag ctcatcgtcg ctctgctgct cgtttgaggg
     2521 cactggcgcg ccgatcggtg cgggcacact cggggcatta acgaaaccga ttgactgcat
     2581 cgatctggcc gtcctcagaa cgtcggaagc cgatgtcggc tggatgtcac aacccgaaag
     2641 ggtcacgttg aaaacttcgc gcatcgcatt atcgagcacg atctggttcg tatgcgacgg
     2701 ctgcgcgctg tcatccagca cgggtcgcag atttgggcaa ctcggaaagc ggaacagctt
     2761 gcccgtgctg ctgccggttg gaaacgcctc caccccggct ggcccgggct ccagaacgtg
     2821 atagctaacc agccgtccgt catcaagagc gagctgggtg aatatacgcc ggattgcgtt
     2881 cggtgcgtcg gcgttgaacc cggcaaacgc tggcgctgtc agagttagcg cggcggcgca
     2941 aaacgcggcc agcgcatggc gtgcataaga ttttctattc atagtgccag cacgctatgc
     3001 ccaggcgatc tccccaacaa cgaatcaatg gttgcagagt tgttactttt gcgacgcact
     3061 gttgccacaa gcgaatcgtt gctatcactg ggcaatccga gcatcagttt catgctgcgc
     3121 ctccggcgcg atcattttgc tggagcggct tgctgcgttc gcacaacgaa gcgtttttcc
     3181 aatgacttct cttatttaaa gatgttgtcg aagagtagtt gcgcccggct gtagttgcgg
     3241 ccgtgcgggc cctgatcgtt caagccccag ttgtacggaa aatatttggt ctcgaatttg
     3301 ccactggcca caaattcgcg caccgattgc ggggcgtata ggcccagaat t
//
LOCUS       DASQUQ010000100         4918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1294062, whole genome shotgun sequence.
ACCESSION   DASQUQ010000100 DASQUQ010000000
VERSION     DASQUQ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294062"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VE486_02760"
     CDS             complement(<1..236)
                     /locus_tag="VE486_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38038.1"
                     /translation="MVSVTEDKLEAIRAAFPKEGLFAEKDWLLSPDAFSIEERFIAEL
                     EQLGHRLFVFQRACNQLYQLSVKGKQPAWVKRYL"
     gene            complement(382..993)
                     /gene="ruvA"
                     /locus_tag="VE486_02765"
     CDS             complement(382..993)
                     /gene="ruvA"
                     /locus_tag="VE486_02765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZA38039.1"
                     /translation="MITFLDGKLVNALPTQAIVDVGGVGYEVFIPLSSYDKLPSAGQP
                     IRILTHLAVREDAHVLYGFMTAAERDLFRLLVNNVSGIGPKLALAVLSGMSVNNFKAA
                     VVNSDVASLSKISGLGKKTAERIILELKDKLGVAAAWEAASAMHAPTAEQEQANEAVL
                     ALIALGYKQIDAHKAVRDLQEKGEATSAEELVKLALKRMAAGR"
     gene            complement(1172..2749)
                     /locus_tag="VE486_02770"
     CDS             complement(1172..2749)
                     /locus_tag="VE486_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZA38040.1"
                     /translation="MNPATPINHKPATIAGIRNYALLFVGCVLFHIAGTWNLPLIDRD
                     EPRFAEASREMIERGDYVVPYFNNHLRLDKPPLTYWAQIASYKIFGENDFAARFPSVI
                     AAALTALSIFAWGSRLRLPWPPSAAQARNGGARVGWWAAIVFTLSLQTFLHAKAAVAD
                     MWLVLFVTLAHWAGYEMLRDRLSPPADQTSNIKHQTSYWWWLFYLSLAFAFLAKGPIG
                     WVPLLTVGLTMIFLREMPSARRFAFGRGLLLMFAVIAAWGIPALIQTHGQFLTVGIGR
                     HVIGRSLATMEGHGANSFGMYLLLLPFYFVTILLSFFPWSIKLPWLIRKLWRRQAASV
                     GGPGDNARDKIDSYLIAGVAIIFIIFTLVKTKLPHYTLPAFPLLALLLARRLTAENAT
                     RFFRNCALASASAYLAIALIVPPFVSRLFPAYALFQESRSYLQPDMQFASVDFEEPSL
                     VWYFRSQVHGFLVPLNNRRAADFMQSSGSRFVVLPTSIAGTLFAEHPEDWKMFSIHGF
                     NIAKGKRVDLTLVLKPE"
     gene            2797..3444
                     /locus_tag="VE486_02775"
     CDS             2797..3444
                     /locus_tag="VE486_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZA38041.1"
                     /translation="MKRSSVFYWLLGIMVVAIVVTAGFYFDNAVHDFIAQHRNREVFN
                     FMYNVTRFGDWPAHVAVGLILAGVAWRLGNKKWTRIFLSMLIALALAGVIGTIIKRGI
                     PRARPSVKSELRWGGPRFSTHHHAFPSGHVAASTAFFGVLFFARRRRIALACLPIPVL
                     IGFSRMYVAAHYLSDVVCAAILGILCALLVTRVLLPRNEHRTSNIEHQTSNSRRG"
     gene            complement(3433..3517)
                     /locus_tag="VE486_02780"
     tRNA            complement(3433..3517)
                     /locus_tag="VE486_02780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3481..3483),aa:Ser,seq:tga)
     gene            3670..4797
                     /locus_tag="VE486_02785"
     CDS             3670..4797
                     /locus_tag="VE486_02785"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HZA38042.1"
                     /translation="MAKEATRKSRKKFVPVIEEAPQSREPIHGRGASWSPANRFEKLH
                     VDVNEFDVVDVDPDENERPRRDTQFFRDGTKTIITHNNSPDVGFETSLNPYRGCEHGC
                     IYCYARPTHEYLGFSAGLDFESKIMVKTNAPELLRAELERPGWKPQVLVMSGVTDPYQ
                     PVEKKLRITRGCLEVLAKFRNPVAIITKNRLVTRDADLLRELAKYHAVAANISVTSLD
                     PKLQRVLEPRTSSPRARLDTISQLRAAGIPVGVMVAPVIPGLTDHEVPKILEACAKAG
                     AQFAGYTIVRLPWAVAPLFEHWLEEHFPDRKEKVLGRIRHLRGDRLNNSQWGTRMTGE
                     GIFTEQIASLFEVGCRRAGIGGRPKLSTAAFRRTREQLTLL"
BASE COUNT         1160 a         1335 c         1368 g         1055 t
ORIGIN      
        1 tcgaggtaac gcttcaccca cgcaggctgt tttcctttga cgctcaattg ataaagctga
       61 ttgcaagcgc gttggaaaac gaacaaccga tgtccaagct gctcgagctc ggcaataaat
      121 ctctcttcga tcgaaaaggc gtcgggtgaa agcagccaat ccttctccgc aaagagaccc
      181 tccttaggaa aagcagcgcg gattgcttct aatttatcct cggttacgga gaccatcttt
      241 gcggactaac gtccaacgtc caacgtccaa cgtccaaccg gaagataggc ttcgagcctg
      301 tctcggcaga catgcatctt gcgtgtcgaa tccgccgcgc cgacaagctc ggaagcctgt
      361 cggccgagac agccaggatg gctatcttcc cgccgccatg cgtttcagcg ccagtttcac
      421 cagttcttcc gccgacgttg cttcgccttt ttcctgcaag tctcgaaccg ccttgtgcgc
      481 gtcgatttgt ttgtagccga gcgcgatcaa cgcgagcact gcttcattcg cctgctcctg
      541 ctcagctgta ggcgcatgca tcgcgcttgc cgcttcccag gccgcagcca cacccagctt
      601 gtctttcaat tccaatataa ttcgctcggc cgtctttttt cccagcccgc tgattttcga
      661 tagcgacgcg acatcagaat taacgactgc agccttgaag ttgttcacgc tcattccact
      721 caacaccgcg agcgcgagct tcggcccgat cccgctcacg ttatttacga gcaatcggaa
      781 aaggtcgcgc tccgccgcgg tcataaagcc atagagcaca tgcgcgtctt cgcgaacggc
      841 caaatgagta agaatacgaa tgggttgacc ggccgatgga agcttatcgt aactcgagag
      901 aggaatgaac acttcgtagc caacgccgcc gacatcgaca atggcctggg tgggcaaagc
      961 gttgacgagt ttgccgtcaa gaaaagtgat catgggatgt taaaaaggtt gaagcgttaa
     1021 agcggggata cgttatttgt cattctgagc gaagcgaagg atctcattat ttctgggcgc
     1081 ccatccgccg caagccgata gtcaaagatg tttcgctccg ctcaacatga cagaccgcgg
     1141 aaccgcttca acgctttagc gttccaacga gtcactccgg tttcagaacg agcgtaagat
     1201 cgacgcgttt acccttggcg atgttgaagc cgtggattga aaacattttc cagtcctcag
     1261 gatgctccgc gaaaagcgtt ccagcgatcg aagttggcag aaccacaaag cgcgaccccg
     1321 agctctgcat gaagtcggca gcgcggcgat tgtttaatgg cacgagaaaa ccgtgcacct
     1381 ggctgcgaaa ataccagacc aggcttggct cctcgaaatc gaccgaggca aattgcatgt
     1441 ccggttgcag atagcttcgc gattcctgga aaagtgcata ggccggaaaa agccgcgaga
     1501 cgaacggcgg aacgataaga gcaattgcaa gataggccga ggcggatgca agagcgcaat
     1561 ttctgaagaa acgtgtcgcg ttttccgcgg tcaacctgcg agcaagcaaa agagcaagca
     1621 acggaaacgc cggcaatgtg taatgcggca gcttggtttt gacgagcgtg aagatgatga
     1681 aaataatcgc gacaccggcg ataaggtagc tgtcgatctt atcgcgtgcg ttgtcgccgg
     1741 ggccgccgac actggccgct tgccttcgcc acaactttcg gatcaaccac ggcagtttga
     1801 tcgaccaagg aaaaaaactt agcaagatcg ttacgaaata aaacggtagc agcaggagat
     1861 acatcccaaa agagttcgca ccatgacctt ccatcgtggc gagcgagcga ccgataacat
     1921 gccggccgat tcccaccgta agaaattggc catgcgtctg aattagtgca ggaattcccc
     1981 aggctgctat gaccgcgaac atcagcagca gcccgcggcc aaaagcgaat cgacgagcac
     2041 tcggcatttc gcgcagaaag atcattgtca atccaacggt caacagcgga acccatccga
     2101 ttggtccctt ggcaagaaat gcgaacgcca gcgacaagta gaaaagccac caccaataag
     2161 atgtttgatg tttgatgttt gatgtttgat ctgctggtgg tgacaaccga tcgcggagca
     2221 tctcgtatcc ggcccagtgt gccagggtga caaacaaaac gagccacatg tctgccacgg
     2281 cggctttcgc gtggagaaat gtttgcagcg agagggtgaa aacgattgcc gcccaccatc
     2341 caactctggc cccgccattc cgcgcctgcg ccgccgaagg tggccacggc aaccgaagac
     2401 gcgagcccca ggcgaagatg gacaatgcag tcagggcggc ggcgattacc gatggaaaac
     2461 gagctgcgaa atcgttctcg ccaaaaattt tgtaactggc gatctgcgcc cagtaggtga
     2521 ggggaggctt atcaagccgg agatgattgt tgaagtaggg aacgacataa tcgccgcgct
     2581 caatcatctc gcgcgatgct tcagcaaaac gcggctcatc acgatcaatc agcggcaaat
     2641 tccatgtgcc ggcgatgtga aaaagcacac agcccacgaa caggagcgcg taattgcgaa
     2701 taccggcaat cgttgccggc ttgtgattta tcggagttgc agggttcaca cggcgccctg
     2761 aaaatagcca ccggcaaatc tgacgggaac ttatttgtga agcgcagctc tgttttttat
     2821 tggctcctgg gcatcatggt tgtggcgatc gtcgtcacgg ccgggtttta cttcgataac
     2881 gccgtgcatg atttcatcgc gcagcatcgg aaccgagaag tcttcaattt catgtataac
     2941 gtgacccgat tcggcgattg gcctgctcac gtcgccgtcg gtctcatcct tgccggagtc
     3001 gcctggcggc tgggcaacaa aaaatggaca cgcatctttt tatcgatgct aattgcgctg
     3061 gcgcttgcgg gtgtaatcgg gaccatcatt aaacgcggga ttccgcgcgc ccgcccctcg
     3121 gtgaaatcgg agcttcgttg gggtggcccg cgtttcagta cgcatcatca cgcatttcca
     3181 tccggccacg tggctgcatc gacagcgttt ttcggggtcc tatttttcgc aaggcggcgg
     3241 cgaatcgctc tggcctgttt accaatcccg gtcctgatcg gtttctcgcg gatgtatgtc
     3301 gcggcgcatt atttgtccga tgttgtttgc gccgcgattc tcggaattct ttgtgctctg
     3361 ctggtgacgc gggtgctact cccgcggaac gaacatcgaa cctcaaacat cgaacatcaa
     3421 acgtcgaact cgcggagggg gtgagattcg aactcacgag gcctttcggc cttccggttt
     3481 tcaagaccgg cgcaatcaac cgctctgcca cccctcctgg gaaaaagtga tcagtggatc
     3541 ggccgttatt atagcagcat tactccatca ctccattcct ccaagtgagc gaggaattgg
     3601 ggcagcggag cggagtgttg aaaagcaatt cggctcgaca cacggctatg ttcatgcgaa
     3661 cgtgtggcca tggcaaagga ggcgacgcgc aaaagccgga agaagttcgt ccccgtgatt
     3721 gaggaagcgc cgcagagccg cgagccgatc catggacggg gagcttcgtg gagtccggcc
     3781 aaccgattcg agaaactgca tgtcgatgtt aatgagttcg acgtcgttga tgtcgatcct
     3841 gatgaaaacg agcggccgcg gcgcgacacg caattctttc gcgacggcac caagacaatc
     3901 atcacgcaca acaacagtcc ggacgtaggc tttgaaacga gtctgaatcc ctaccggggt
     3961 tgcgagcacg gttgcattta ttgttacgcg cgaccgactc acgaatatct gggtttttcc
     4021 gccgggctcg atttcgagag caaaattatg gtgaaaacaa atgcgccgga attattgcga
     4081 gccgaattgg aacgaccagg atggaaaccg caggtgctgg tgatgagcgg cgtgactgat
     4141 ccttaccagc cggttgagaa aaagctgcgg attacgcgcg gttgtctcga ggtgctagcc
     4201 aagtttcgta acccggtggc gattatcacc aagaaccggc tcgttacgcg agatgccgat
     4261 ctgctgcgcg agcttgcgaa ataccatgct gttgcggcga acatttccgt gacgtcgctc
     4321 gatccgaaat tgcaacgtgt gcttgagcca cgcacgtcgt cgccccgggc gcgcctcgat
     4381 acaatcagcc aacttcgagc cgcaggaata ccagttggcg tgatggttgc gccggtcatt
     4441 cccgggctca cggatcacga ggttccgaag attctcgagg cctgcgcgaa agcgggcgcg
     4501 caatttgccg gttacacaat cgtgcgattg ccatgggcgg tcgcgcccct tttcgaacat
     4561 tggctcgaag aacatttccc tgatcgcaaa gagaaagtgc tcgggcgcat ccggcatttg
     4621 cgcggagatc ggctcaataa ttcgcagtgg ggcacccgca tgactggtga aggcattttt
     4681 accgagcaga ttgcttcgct tttcgaagtt ggttgtcgtc gtgccgggat cggtgggcgg
     4741 cccaagcttt cgacggccgc attccggcgg acgagagagc aactgacgtt actttagttt
     4801 aatcggaagc ggaaccgata tctcaaaatg gagcttgcct ccgtgtaagc cgagttttaa
     4861 aacgaacgaa tgatgctcga aactttaaaa cgtcattggt gggttccggt gctgcggg
//
LOCUS       DASQUQ010000101         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_197355, whole genome shotgun sequence.
ACCESSION   DASQUQ010000101 DASQUQ010000000
VERSION     DASQUQ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197355"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..596
                     /gene="thpR"
                     /locus_tag="VE486_02790"
     CDS             48..596
                     /gene="thpR"
                     /locus_tag="VE486_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZA38043.1"
                     /translation="MKRLFVAIDLPESTRQFLADLDPHIRGVRWTETEQMHLTLGFFG
                     DVPEDVDLALREELSAIDFGAFFLPITGAGTFPPKGPPKIIWIGVGRGHPHLFQVHKR
                     VQEAALAVGIEADLRPWHPHITIARCRDVAPQSIRKFLQSNVDRDLGMIRVDSFHLYS
                     SKLTPAGSIHTRELTVNCSRER"
     gene            complement(658..2112)
                     /locus_tag="VE486_02795"
     CDS             complement(658..2112)
                     /locus_tag="VE486_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZA38044.1"
                     /translation="MADGETIESYVPARLDRMPWSRWHWLIVVSLGATWILDGLEVTL
                     AGALGGILTRHETLGLTDTQVGASATCYLAGAVLGALLFGYGTDRFGRKKLFFITVAV
                     YLIGTALSGFSWNFASFAIFRALTGAGIGGEYAAINSAIDELIPARVRGRVDLIINGS
                     YWIGAAIGAAATLVLLDPRWLPIWLGWRCAFGIGALLGLIVIFFRRWIPESPRWLMIH
                     GRNHEAEQIVSEVERKIAGTTPAAVIPTSRDYSDVPPTRIRTRTHTPWPEIWNAIVHE
                     HRRRSFLGFVLMLTQAFFYNAIFFTYALVLMRFYGVPEQSVGRYLLPFALGNALGPLL
                     LGHLFDTIGRRQMIAATYGLAGILLALTGWLFHAGLLTAQTQTLAWTIIFFIASAAAS
                     SAYLTVSEIFPLEVRALAIAIFFAIGTLAGGVGAPVLFGWIIGTGSVTALFIGYLLAA
                     GLMIFGAVVEVWIGVPAERQPLEHVAAPLSSKGL"
     gene            complement(2160..2519)
                     /locus_tag="VE486_02800"
     CDS             complement(2160..2519)
                     /locus_tag="VE486_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38045.1"
                     /translation="MYLSILLAFYDIGPHIMGGERVTREQWLHIAAPLVAVIGVLMAC
                     ISYGFAKEKAWSRHLVIAMFALIVVYASAFGALNLMHRPIMWRAIINGTLFGGAAVWY
                     FYFKPNVADYFQQLANR"
     gene            complement(2597..3403)
                     /locus_tag="VE486_02805"
     CDS             complement(2597..3403)
                     /locus_tag="VE486_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38046.1"
                     /translation="MDNFICIQCGTQFDETAQRPSSCPICEDERQFVRHAGQEWTKLD
                     QLARDHHNRFELEAPQLLGIGTEPEFAIAQRTLLLQSPGGNLLWDCISLLDDETVAEV
                     SARGGIRAIAISHPHFYSSMVDWAERFDAQIFLHAADREWVMRKSPRIQFWEGSAHVL
                     WDDLTLIHCGGHFEGGTVLHWPEGANGKGALLTGDIITVAQDRRYVSFMRSYPNLIPL
                     GPAAIRRIVERIETFSFEQIYGGWWQANVLADAKAAMARSAERYLRAISH"
     gene            complement(3534..4181)
                     /locus_tag="VE486_02810"
     CDS             complement(3534..4181)
                     /locus_tag="VE486_02810"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HZA38047.1"
                     /translation="MKKTAVVVLAACVVLATGCRWVGIRGNGNIKSDERTIGAFANID
                     AGGAFQIEWQNGEPALRITTDENLLPYIENQISGDTLRLRTREQIWPTHGVKIVISSH
                     TRNGARISGAVKLTANQLSSPKFAFEATGASQVKLDGTIDQLLADMTGASELNASGLQ
                     TRTAEISTTGAADAEIAVSETLKVAITGAGKVTYSGNPKTIEKHITGAGSIRHKD"
BASE COUNT         1026 a         1307 c         1198 g          916 t
ORIGIN      
        1 cgtctggctg atcgcgcgac cattcgcaac aacgaagagc gccggaagtg aaacggcttt
       61 tcgttgccat cgatcttccg gaatcaacgc ggcaatttct tgccgatctc gatccgcaca
      121 ttcgcggagt gcgctggacc gagaccgaac agatgcatct gacgctcggc ttcttcggcg
      181 acgttccaga agatgtcgat cttgcgctgc gtgaagaact cagcgcaatc gactttggag
      241 cattttttct acccatcacc ggcgccggga catttccgcc gaagggcccg ccgaaaataa
      301 tttggatcgg cgttggtcgc ggacatccgc atcttttcca agtgcacaag cgcgtgcaag
      361 aagccgccct ggccgtcggg atcgaagccg atctccgccc ctggcatccg cacattacga
      421 ttgcgcgttg ccgagatgtc gccccgcaat cgattcggaa gtttttgcaa tcaaatgtgg
      481 atcgtgacct gggaatgatt cgcgtcgatt cgtttcatct ttattcgagc aaactgacgc
      541 ccgccgggtc gattcacacg cgcgagctta cggttaactg tagccgcgag cgatgaccgc
      601 ggcccccccc gcaggtccga gccggactgg cgaatacggc agcggctaca acctccgcta
      661 caaacccttg ctcgagagtg gcgcggccac gtgctcaagt ggctgccgtt cggccggcac
      721 gccaatccag acctcaacca ctgcgccaaa aatcataagc ccggctgcga gcagatatcc
      781 tatgaacaac gccgtaaccg atcccgtgcc gatgatccat ccgaacaaca cgggtgcgcc
      841 cacgccgccc gccagtgttc caattgcgaa gaaaattgcg atcgccagag cgcggacttc
      901 caacggaaaa atttcgctta ccgtcaaata cgccgagctg gcagcagccg acgcgatgaa
      961 aaaaataatc gtccaggcca acgtttgtgt ttgcgcggtg agcaagccgg cgtgaaaaag
     1021 ccagcctgtc agcgcgagca aaatgccggc gagcccgtag gtcgcagcga tcatttgtct
     1081 gcgtccgatc gtgtcaaaca aatgcccgag caggagcggc ccgagcgcat ttcccagcgc
     1141 gaacggcagg agatagcgac cgacactttg ctcaggcacg ccataaaacc gcatcaacac
     1201 gagcgcgtaa gtaaaaaaga tcgcgttgta gaagaaggcc tgtgtcagca tcaacacaaa
     1261 accaagaaat gaacgccgcc gatgttcgtg cacgatggca ttccagattt ccggccaggg
     1321 cgtatgcgtg cgcgttcgaa tccgcgttgg cggcacatcg ctgtagtccc gactcgtcgg
     1381 gatgaccgcg gccggagttg tgcccgcgat ctttcgctcg acttcgctaa caatctgttc
     1441 cgcctcgtga ttccttccat ggatcataag ccagcgcggg ctttctggaa tccatcggcg
     1501 aaagaaaatc acaatgagac ccagcagggc gccaatcccg aatgcgcacc gccatcccag
     1561 ccagattggc agccagcgcg gatcaagcaa cacaagcgtc gcggccgcgc cgatggcggc
     1621 gccaatccag tacgagccgt taattatcaa atcgacccgg ccgcggacac gcgccggaat
     1681 caactcgtcg atcgccgaat tgatcgccgc atattctccg ccaatcccgg cgccggtcag
     1741 cgcacgaaaa atagcaaagc tcgcgaagtt ccaggaaaac ccggacaacg ccgtgccgat
     1801 caggtaaacg gcgaccgtta taaagaacaa tttcttccgg ccaaaccggt ccgttccgta
     1861 gccaaagagc aatgcgccca gcactgcgcc cgcgagatag caggtcgcgc tcgctccgac
     1921 ctgcgtgtcg gtcaatccga gcgtttcatg gcgcgttaaa atcccgccta acgcaccggc
     1981 cagcgtgact tccaaaccat cgagaatcca ggtcgcgccg agtgagacaa caatcagcca
     2041 atgccaccgg ctccagggca tccggtccag ccgcgccggg acgtatgatt cgatggtttc
     2101 tccgtccgcc atatctttcc atcaagtaag ttgcagccac ggctctgcga gccgcgagat
     2161 catcggtttg cgagttgctg aaaatagtcc gccacgtttg gcttaaaata gaagtaccaa
     2221 accgccgcgc cgccaaacaa agtgccgttg ataatcgcgc gccacataat cgggcgatgc
     2281 atcagattga gcgcgccgaa ggccgacgcg taaacaacga tcagggcaaa catcgcgatc
     2341 acaagatgac gcgaccacgc tttttccttc gcaaacccat aggagatgca tgccatcaga
     2401 acgccaatca cagcgacaag cggcgcggcg atatgcaacc attgttcacg cgtgacgcgt
     2461 tcgccaccca tgatgtgtgg tccgatatcg taaaacgcca gcaagatcga caaatacatg
     2521 ccggctgaag caagaccagc aaaccacctc aaaatccgaa tcactaatgg ttgcctggaa
     2581 tttggcacga caataactag tggctgatcg cccgcagata gcgttccgcc gagcgcgcca
     2641 ttgcggcttt agcgtcggcc aagacgttcg cctgccacca gccgccgtag atttgctcga
     2701 atgagaatgt ttcgattctt tcgactatgc ggcggattgc cgcgggcccg agtggaatca
     2761 ggttcggata acttcgcata aaactgacgt aacggcggtc ctgcgccacg gtaatgatat
     2821 cgccggtaag caacgcgcct ttgccatttg cgccctctgg ccagtgcagc accgtgcctc
     2881 cttcaaaatg accgccgcaa tgaatcagcg tgagatcgtc ccagagcacg tgggcgcttc
     2941 cttcccaaaa ctggatgcgc ggactcttgc gcatgaccca ttcgcgatcg gcggcatgca
     3001 aaaaaatttg ggcatcgaaa cgctcggccc agtcaaccat cgaagaataa aaatgcggat
     3061 gcgaaatagc aatggcacgg atgccgccgc gggcgctcac ttcggcaacg gtttcgtcgt
     3121 ccagcaagga gatgcaatcc cagagcagat ttccgccggg cgactgcaaa agcaatgtgc
     3181 gttgcgcgat ggcaaattcc ggctcggtgc cgatcccgag aagttgtggc gcttcgagtt
     3241 caaagcggtt gtgatgatca cgcgcgagct gatcaagctt ggtccattcc tgaccggcgt
     3301 gacgaacgaa ttgccgttcg tcctcgcaaa tagggcatga ggatggccgt tgtgcagtct
     3361 cgtcaaactg cgttccgcac tggatgcaga tgaagttatc cacggcgtga tgagcctaac
     3421 ccgataatcg ccgctgggaa aaatcgacgt agaggtgctc gccacgtcca gcacctgact
     3481 aattggtttc gacgttcgcc gcggcgaacc cccgctagaa gcatttctct cagctaatcc
     3541 ttgtgccgaa tcgagccggc accggtgatg tgtttctcga ttgtttttgg attgccggag
     3601 taagtgactt ttcccgctcc cgtgatagcg accttcaatg tctcgctgac cgcgatctcc
     3661 gcgtcggctg cgccggtggt tgagatttcg gcggttctgg tttgcagacc gctggcattt
     3721 aactcacttg cgccggtcat atcagccagg agttggtcga tggtgccatc gagtttaact
     3781 tgcgatgcgc cggtcgcttc aaaagcgaat tttgggctgg agagttggtt cgctgtgagc
     3841 ttcacagcgc cgctgatcct cgccccgttt cgcgtatggc ttgaaatcac gatcttgacc
     3901 ccgtgcgtcg gccaaatctg ctcgcgcgtg cgcaagcgaa gcgtgtctcc ggagatctga
     3961 ttctcgatgt agggaagaag attttcgtct gtcgtgatac gcaaggcagg ttctccattt
     4021 tgccattcga tttgaaatgc gccaccggca tcgatgttgg caaaggcgcc aatggtgcgt
     4081 tcatcggatt tgatattgcc gttgcctcga attcctaccc accggcatcc ggtagccaag
     4141 acgacacatg cggccaaaac tacgactgcg gtcttcttca tggaaatatg cctcctgttg
     4201 ttaggatgca gggagcgttt gctttggatt caagaaattc tgcgtcgcaa cgcttttgtc
     4261 atgtcgagcg aagtcgagac acctctcact gttaatgttt ttgaatagta agagattcct
     4321 ccagccttcg cataaagcta cggcttggca ggcaacttcg ctcggaatga caaagagcgg
     4381 gttaaagctt ttcaacagag acccaaagct tctacattga atgaccgatt atttgttagt
     4441 cgatatc
//
LOCUS       DASQUQ010000102        10410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_747720, whole genome shotgun sequence.
ACCESSION   DASQUQ010000102 DASQUQ010000000
VERSION     DASQUQ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747720"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VE486_02815"
     CDS             <1..543
                     /locus_tag="VE486_02815"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38048.1"
                     /translation="YILMENANGLVLVDQHAAHERILFEELRRKMEEQGVPTQKLLLP
                     QTFDLPPRDADWIERNLSTLEKMGIGIESFGPNTFKIDSLPAFLNVLDPAQFMSKVID
                     DLRSASNSSSAMRMGEEMIAKTVCRHAVKANDPLGYLEVEKLIRDLLDCDLPYCCPHG
                     RPTMIQISLAELEKKFGRKI"
     gene            914..1912
                     /gene="gmd"
                     /locus_tag="VE486_02820"
     CDS             914..1912
                     /gene="gmd"
                     /locus_tag="VE486_02820"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZA38049.1"
                     /translation="MKDSEKIAFITGITGQDGSYLSELLLEKDYVVHGIVRRTSNLVR
                     SRIEHLRRDDRIYGQRLFLHYGDLGDSTTLRRIVSDVRPDELYHLAGQSHVGLSFEIP
                     ESTCEEAGMATLRLLEIARDQPQPVRFYNASSSEIYGNASETPQTEETPLRPTSPYGC
                     AKAFATQLARIYRQSYGLFVCNGILYNHESPRRGENFVTRKIARAAARIARNLDEEVA
                     LGNLESQRDWGRAQDYVQAMWLMLQHETADDYVVATGEIHSVHDFVEAAFAVVDLPWE
                     KYVKHDPSFDRPTEPTRLVGHAEKIRKTLGWKPTGDFGQLVREMVEAELAAIDSRQ"
     gene            2030..2368
                     /locus_tag="VE486_02825"
     CDS             2030..2368
                     /locus_tag="VE486_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZA38050.1"
                     /translation="MKKIEAIIKPFKTEPVKEALLAVGVEGMSLSEVKGFGRQKGHSE
                     IYRGTEYTVDFLPKVKFEMVVSDDQAQRAVEAILGAAKTGKIGDGKIFVVEVEEAVRV
                     RTGERGPEAL"
     gene            2433..3290
                     /locus_tag="VE486_02830"
     CDS             2433..3290
                     /locus_tag="VE486_02830"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZA38051.1"
                     /translation="MNDYLLSILLGIIEGLTEFLPVSSTAHLRICEALLHIDLKDGFW
                     KMYTIVIQLGAILALPVYFWHRIVQFVRTFPKGERGNRTILTHPLSLTLIAFVFTAVP
                     AWALSKQIGKNLENLWVMALALLIGGIIMWIVDTVFTRPRTMDMEEMNLPQAVWIGTA
                     QTLSAIFPGTSRSMCTIAAGQVAGMSRPAALEFSFFLSMPTMFMATGYDFVKTVVPHH
                     SSNIAPLTMSGHQWIVLGIGFVVSFLSALGVVAWFMHWVRHRGFVPFAIYRIVLGITL
                     LILLMRGLM"
     gene            3424..4692
                     /locus_tag="VE486_02835"
     CDS             3424..4692
                     /locus_tag="VE486_02835"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZA38052.1"
                     /translation="MANTILIGAQWGDEGKGKIIDVLTGKADIVVRSQGGNNAGHTVI
                     HRGVKYVLHLIPSGILRRSKICVIGNGVVIDPSALVTEINGLRNLGITIGKNLLISDC
                     AHLVLPYHRLIDEQRELRQGRARIGTTKRGIGPAYGDKAARTGLRMSDLMQPIVFSKK
                     LQAKVLENNKILQALGAKPISFREVNESYLKAGENLRPFVSNTVVYLHRALERGKEIL
                     FEGAQGTFLDIDHGTYPYVTSSNTTAGGACTGTGVPPHRMDRVVGVMKAYTTRVGEGA
                     LPTEDLQLAETLHSLGREFGATTGRKRRCGWFDAVATHYATMLNGIDELAITNLDGLD
                     DVDPIRICVAYRLNGKRLDVPPCDAAQLANCEPVYEEVSGWKRSTHSARKFSQLPSAA
                     RKYIKYISKLTGAKLSIVSVGPARGQTIVL"
     gene            4708..5412
                     /locus_tag="VE486_02840"
     CDS             4708..5412
                     /locus_tag="VE486_02840"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HZA38053.1"
                     /translation="MKTVPRHIAIIMDGNGRWARARGLPRIKGHEAGAQAVRECVEGC
                     GELKVEYLTLYAFSAENWQRPRGEVHSLMRLLEKFLKEKTPELIEKNVRLQAIGRLTD
                     LPESSQKQLHKTIEQTAGNNGLTLILALSYGGRLEIIDGIKSLLRAVELGHIDKGMID
                     VEMFSKHLYTRYYPDPDLLVRTSGEMRLSNFLLWQLSYTEIYVTPKLWPDFTKKDLLA
                     AVEEYGRRQRRYGAVE"
     gene            complement(5477..6670)
                     /gene="hemW"
                     /locus_tag="VE486_02845"
     CDS             complement(5477..6670)
                     /gene="hemW"
                     /locus_tag="VE486_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZA38054.1"
                     /translation="MSDGVIHNSSAVVEAVSAAFPSPAGDTSANTEPIRYIYVHIPFC
                     ARICPYCAFYKDLLDRSQTQRFCEALLGELDGQRAIFAISPKTIYFGGGTPTALSTSQ
                     LEFLLGGFRERLDLSALEEWTIEANPGSVSARKAALLCKLGVNRISLGVQSWDNTLLK
                     ILGREHNAEQAEQSFHILRAAGFSNINIDLMFGLPGQTVHQWQQTLEKTIWLRPDHIS
                     AYCLTYEEDTEFFLRQSRGELKANPDTDADFFEVGMSILEEAGYEHYEISNYARPGFT
                     SVHNRAYWSGEDYLGVGPSAFSTVGMRRWQNLADYRGYADRILLGQSPIGATENLTTA
                     MKRAERIALSLRTRDGISASELRRFARETNEFIALGLLRQSSGKFILTQKGKSLADSV
                     AEALV"
     gene            6703..7743
                     /locus_tag="VE486_02850"
     CDS             6703..7743
                     /locus_tag="VE486_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZA38055.1"
                     /translation="MRNVALVLFCAIASGFGQGAQPAATPSIVFAVRDPDAIKGYKTN
                     PRVVRAMVNRLVLAATGQPDVARAWGSLVSPSDKIGIKISAAGGELFTTHHDVVNAIV
                     DGLAAAGHPRGSIIVWDRSLGGMKGAGYRPDIGGYQLKAIAPHTGYDPKAVLSAPLIG
                     KLIWGDLDYRSYEGKMPLLSDSENTSNVSHFSKIISTEVTKIINVPVMSSSEMNGIAG
                     CIYNVTIPNIDNWRRFAQGSRFGAESLAEIYGDPVIAKKVVFNLMDGLVAQYAGGPQS
                     QPNYAVHHATLYASKDPVALDAVALRRLEEWRARASLPPIGRTAAYIDFATQLGLGNS
                     APNRIEVRNIGR"
     gene            7766..8779
                     /locus_tag="VE486_02855"
     CDS             7766..8779
                     /locus_tag="VE486_02855"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HZA38056.1"
                     /translation="MSAQSRHSVQELQTAILRSVSRSFYLSIRFLPAQLREPVAVAYL
                     LARTTDTVADTAQIPGTVRTETLKMLSNGIQGKASREVVIDLIASFVPLQENASERRL
                     LESLPDCLEWLEHVEAADRDDIRAVLEKIIHGQMLDLQRFDNPTEIRALQSAADLDEY
                     TYFVAGSVGEFWTRLCFRHVRDFASLNEDQMLALGKRYGMALQLINVLRDAGSDLRAG
                     RCYFPEYELGAVHLSADEIFSEQERFQPIYRRWADKARDGLKCGMQYSRAIKNRRVRA
                     ATALPALIGARTLALLHDAGATALQRTVKIPRWEVRRMISSLAVSLASRKRIDAMFEQ
                     LSK"
     gene            complement(8885..9580)
                     /gene="ispD"
                     /locus_tag="VE486_02860"
     CDS             complement(8885..9580)
                     /gene="ispD"
                     /locus_tag="VE486_02860"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZA38057.1"
                     /translation="MLTAIIVAAGSSQRMGFDKLFAPIAGKPVIAHTLQAFERASCVN
                     EIIVVAREDRQDEIRKISHVGNFKKIRSIITGGERRQDSVRIGLGRLSADAKYVAVHD
                     AARPLVTSEQIERVFEKCRDYGAATLAEPIRDTVKRADVDLIVTASVDRHQLYVMQTP
                     QVFERGLIEEAYRAAYTENVSLTDEVSAVERLGRKVVLVLNNDFNFKITYPRDLELAE
                     FVLKQRAHPGRKL"
     gene            complement(9611..9907)
                     /locus_tag="VE486_02865"
     CDS             complement(9611..9907)
                     /locus_tag="VE486_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZA38058.1"
                     /translation="MNLNKLMKQAQKMQEQMAKTQAELEKKTVEVNAGGGKVKVIANG
                     AGDIVAIKIDKEVVDPNDVEFLEEIVLSAVKQAAEQGKALAQEEMTKLTGGLQL"
     gene            complement(10036..>10410)
                     /locus_tag="VE486_02870"
     CDS             complement(10036..>10410)
                     /locus_tag="VE486_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38059.1"
                     /translation="ADIDPAQVWEQLNTKIPSLKAFLRNSAAAAHVLGIEGRNFQLGF
                     SPDQKPLMEILGTQANRKVLETLLYEITGKDLTVKLAVNDDLPSKQTITPAGVEASRS
                     EDFKDDPLIQEALEIFNAKIKS"
BASE COUNT         2344 a         2730 c         2926 g         2410 t
ORIGIN      
        1 tatatcctga tggaaaacgc aaacgggctt gttctggtcg atcagcacgc cgcgcacgag
       61 cgcattcttt tcgaagagtt gcggcgaaaa atggaagagc agggagttcc aactcaaaag
      121 ctgctgcttc ctcagacttt cgatctgccg ccgcgtgatg cggattggat cgagcgcaac
      181 ctgtcgacct tggaaaaaat ggggatcgga attgagagct ttgggccaaa tacatttaag
      241 atcgacagtc tcccggcctt tttaaacgtg ttagatccgg cccagtttat gagcaaagtg
      301 atcgatgatc tcagaagcgc cagcaacagc agctcggcga tgcgaatggg ggaagagatg
      361 atcgccaaaa ccgtttgccg gcatgccgtc aaagccaatg atccgctggg ttatctcgaa
      421 gttgagaaac taattcggga tctgctcgat tgcgacctgc cttattgctg tccgcacggg
      481 cggccgacga tgattcagat ttcgctggcg gagctggaga aaaaattcgg gcgcaaaatc
      541 tgattggacc catctcacaa atagggaacg cgtgcgttgc tggagatttg acgctgcagc
      601 aaggcgcgag gagggagaat atccttcatg gatctgtgac cgacgagcaa cgccgctgca
      661 gcggcaaaga tccgcaaccc tgtgggctaa gctctggcgg gccgctggcg gcgttgctcc
      721 tcagtcacag agcgcatatt tggctatgct cctttgtcgc gccttaccag ccgcccgcga
      781 gcgcttagcg cactcattct ctaattgtga gatgggttcc aatacgtgtg ctggcagtga
      841 gtggcgaaat agatcttctt attttctttt cttgcgatta tgaagctcac aagacatggg
      901 tttccgatct taaatgaaag attccgagaa gatcgcgttc attacgggaa ttactggaca
      961 agacggctcc tatttatctg agctgctcct ggaaaaggat tatgtcgtgc acggaatcgt
     1021 gcgacgcacg agcaatctgg tgcgatcgcg catcgagcat ctgcggcgcg atgacagaat
     1081 ctacggtcaa cgattattcc ttcactatgg cgatctcggc gacagcacga ccttgcgtcg
     1141 cattgtcagc gatgttcgtc cagacgagtt gtatcatctc gccggacaaa gccacgttgg
     1201 cctgagtttc gaaattcccg aatcgacctg cgaggaagcg ggaatggcca cgctgcgctt
     1261 gcttgaaatc gcgcgcgatc aaccgcaacc ggtgagattt tataacgcct cgagctccga
     1321 aatttacgga aacgcgtccg aaacgccgca aacggaggag accccgctgc gaccgacgag
     1381 tccctatggt tgcgctaaag cgttcgcgac tcaattggca cggatctatc ggcaatcgta
     1441 cgggctcttc gtttgtaatg gcattctcta caatcacgag tcgccgcggc gcggtgaaaa
     1501 ttttgtgacg cgcaaaattg cgcgcgcggc tgcgcggatt gcgcgtaatc tcgatgagga
     1561 agttgccctg ggtaacctgg agagccaaag ggattgggga agggcccagg attatgtgca
     1621 ggcgatgtgg ttgatgctcc aacacgaaac ggccgatgat tacgtggtcg cgactggaga
     1681 aatccattcc gtccacgact ttgtcgaagc ggcgttcgcg gttgtcgatc ttccctggga
     1741 aaaatacgta aagcacgatc cttcatttga tcgtccgacc gagcccacac gtctggtcgg
     1801 tcatgcggag aagattcgca aaacgcttgg ttggaagccg acgggagatt ttggacagct
     1861 tgtgcgcgag atggtggagg cagagctcgc ggcgatcgat tcacgtcagt gaaggcgcga
     1921 accgcgggcg cattgatttc ctaaactcaa ccctggcagg ctgaattgac cctgctcgtc
     1981 cgtctacgtg ttgcttgcca tttggggcgc ggtctctagt cttggcgcgg tgaaaaaaat
     2041 cgaggcgatc atcaagccct ttaaaaccga gccggtgaaa gaggcgctgc tggcggtcgg
     2101 cgtggaagga atgagcctta gcgaggtgaa aggctttggc cgccaaaaag ggcatagcga
     2161 aatttatcgc ggcaccgaat acacggtcga ttttcttccg aaagtaaaat tcgagatggt
     2221 tgtttccgac gatcaggcgc aacgcgccgt cgaagccatt ttgggtgcgg ccaagaccgg
     2281 caagattggc gatggaaaga ttttcgttgt ggaggtcgaa gaagcggtgc gagtccgaac
     2341 gggcgagcgc ggcccggagg cgctgtgaat cagaggtcag aggcggaggt cggaggtcag
     2401 agatcagaag taacgcctaa agacgacgac gcatgaacga ttatctcttg tcgatcttac
     2461 tcggaattat cgaaggcctc acggaatttc ttcccgtgag ttcaaccgcg catctgcgca
     2521 tctgcgaagc gttactgcac atcgatctca aggacggttt ctggaagatg tacacgattg
     2581 ttattcagct cggggcgatc ctggcgttgc cggtatattt ttggcaccgc atcgtgcagt
     2641 tcgtccgcac gtttccaaaa ggcgaacgcg gaaatcgcac gatcctaacg catccattaa
     2701 gtctgacatt gattgcgttt gtgtttacag cggtcccggc ctgggcctta tcgaaacaga
     2761 tcggcaaaaa tctggaaaac ctttgggtca tggcgctggc gctcctcatt ggtggaatca
     2821 tcatgtggat cgttgacacg gttttcacgc ggccgcgcac catggacatg gaggaaatga
     2881 atctgccgca agcggtctgg attggtacag cacagactct ttccgctatt ttccccggca
     2941 cttcccgttc gatgtgtacc atcgctgctg gtcaggtggc gggcatgtca cgaccagccg
     3001 cgcttgaatt ttcgtttttc ctgtcaatgc ctaccatgtt catggccaca ggctacgatt
     3061 ttgtaaaaac cgtagtgcca catcattcat cgaatatcgc gccgctgaca atgagtgggc
     3121 atcagtggat tgtgctcggc attggctttg tggtttcatt tttgtcagcg ctcggcgtgg
     3181 tagcctggtt catgcattgg gtgcgccaca ggggattcgt cccgtttgcg atttatcgga
     3241 ttgtgctcgg gatcacattg ttgattctgc tgatgcgcgg actgatgtga gaaaagcggg
     3301 aaacctggac agtaatggaa atccccttgc agcggttccc gcccatcata tgattcgtcg
     3361 gcaagtcccg agtgtgtccc accggatgca tcgggaaaat tttttgccca ttttctgatt
     3421 tcgatggcca acacaattct aattggggcg caatggggcg atgagggaaa aggcaagatc
     3481 atcgacgtgc tcacgggtaa agccgacatt gtggtccgca gccagggcgg aaacaacgcc
     3541 gggcacaccg ttattcatcg gggcgtcaaa tacgtcctgc acctcatccc gtcaggaata
     3601 ctccggcgct cgaaaatttg tgtgatcggc aacggtgttg ttatcgatcc gagcgccctt
     3661 gtcaccgaga ttaatggcct gcgaaacctg ggcatcacaa tcggcaaaaa tctgctcatc
     3721 agcgattgcg ctcatttggt tctcccttat caccgcctga tcgacgagca acgcgaactg
     3781 cgtcagggcc gggccagaat tggaacgacg aaacgcggga tcggcccggc ttatggagac
     3841 aaagctgcgc gcactggcct gcggatgtcc gatttgatgc aaccgattgt tttctcaaaa
     3901 aagttgcagg ccaaggtgct cgagaacaat aaaattcttc aggcactcgg cgcgaaaccg
     3961 atcagcttcc gcgaggtcaa cgaaagttat cttaaagctg gagaaaattt gcgccccttc
     4021 gtcagcaaca cggttgtgta ccttcaccgt gctctcgagc gcggtaagga aatcctcttt
     4081 gaaggtgcgc aaggcacatt tctcgatatt gatcacggca cctatccgta cgtgacctcc
     4141 tcgaacacca cggccggcgg cgcatgcacg ggaaccggtg tgccgccgca tcggatggac
     4201 cgggtggttg gcgtgatgaa ggcatatacg acgcgtgtag gcgagggggc gttacctacc
     4261 gaagacttgc aactggccga aacgcttcac agcttgggac gcgagtttgg ggcgacgacg
     4321 ggacgcaagc ggcgctgcgg ttggtttgat gcagtggcaa cgcattatgc aacgatgctc
     4381 aacgggatcg acgagctcgc cattacaaat cttgatgggc tcgatgatgt cgatcccatt
     4441 cgtatctgcg tcgcctaccg gctaaatggg aagcgcctgg atgttccgcc gtgtgatgcc
     4501 gcacagctag ctaattgcga accggtctat gaagaggtga gcggctggaa gagatcgacc
     4561 cattcggcgc gaaaattttc gcagcttccc tcagcagcaa ggaaatacat caaatacatt
     4621 tcgaagctaa cgggagcaaa gctgtctatt gttagtgtcg gcccagcacg cggtcagacc
     4681 attgtcctgt agccaatatt cgcaaacgtg aaaaccgttc cgcgacatat cgcgattatt
     4741 atggatggca atggccgatg ggccagggcg cgcggcctgc cccgaatcaa aggtcacgaa
     4801 gccggcgctc aagccgttcg cgaatgcgtc gagggctgcg gcgaattgaa agtcgaatat
     4861 ctgacgctct acgctttctc cgctgaaaac tggcagcgtc ctagaggtga agtccactcg
     4921 cttatgcgtc tgctggagaa atttttgaag gagaagacgc cggaactaat cgaaaaaaat
     4981 gttcgcctcc aagcgatcgg ccggttgacc gatctccctg agagttcgca gaaacaactg
     5041 cataagacaa tcgagcaaac tgcggggaat aacggattga ccctgatcct ggcgctcagt
     5101 tacggcgggc gactcgagat catcgatggg atcaaaagtt tgcttcgggc ggttgagctg
     5161 gggcacatcg acaagggaat gatcgatgtc gagatgttca gcaaacatct ttacacgcgg
     5221 tattatcccg atccagacct cctcgttcgc acttctggcg aaatgcgttt gtcgaatttt
     5281 cttctctggc agctttcata cacggaaata tacgtaacgc caaaactctg gccggacttc
     5341 acaaagaagg atttgctggc cgcggtagag gaatatggac gcaggcagcg ccgctatggc
     5401 gcggtagagt gacgcggaca atcctgtctg cgtcgttgta catgctcgca ggcacaatga
     5461 tcgcctgcgt cccgacttac actaacgctt ccgcaacgga atcagccagc gatttgcctt
     5521 tctgcgtgag gatgaatttg ccactcgatt gtcgaagcaa gccgagcgca ataaactcat
     5581 ttgtttcccg cgcgaaacgt ctgagttccg aggccgaaat cccgtcgcgt gtgcgcaggg
     5641 aaagggcgat tctctctgcg cgtttcattg cggtcgtcag attttctgtg gctccaatcg
     5701 gcgattgtcc cagcaaaatg cgatccgcat atccgcggta atcagcaaga ttctgccagc
     5761 gacgcattcc cacggtcgaa aacgcgcttg gacccacccc gaggtaatct tcacccgacc
     5821 aataggcgcg attatgaacc gacgtaaaac cgggtcgcgc atagtttgag atctcataat
     5881 gctcatagcc ggcctcctct aggatcgaca tccccacctc gaagaaatcc gcatcggtat
     5941 cgggattcgc cttgagttcg ccgcgtgact ggcgcagaaa aaattccgtg tcctcctcgt
     6001 aggtaaggca gtacgcggaa atgtggtcag ggcgcagcca aatagttttc tcaagggtct
     6061 gttgccattg gtgaactgtc tgacccggga ggccgaacat aaggtcgata ttgatattcg
     6121 agaatccggc cgcacgcaga atatgaaatg attgctcggc ctgctcggcg ttgtgttcgc
     6181 gcccgaggat tttgagcagg gtattgtccc aggactgaac cccgagacta attcgattca
     6241 ctcctagctt gcataatagg gccgccttac gcgccgaaac gctccctgga ttcgcttcga
     6301 tggtccattc ttccaaggcg gagagatcaa gccgctcgcg aaaacctccg agaagaaatt
     6361 ccaattgcga agtactgagc gccgtgggtg tgccaccgcc gaagtaaatt gttttgggcg
     6421 atatcgcgaa aatggcgcgc tgcccgtcaa gttcgcccaa caaagcctca caaaaccgct
     6481 gcgtttggga acgatcgagc agatctttgt agaacgcgca atacgggcag atccgcgcgc
     6541 aaaaggggat atgcacgtag atgtagcgaa ttggttctgt attggcagac gtgtcgcctg
     6601 cgggagaggg aaatgcagcc gacacggctt ccactacagc cgaagagttg tgaatcacgc
     6661 catcgctcat aaaatccggt gccagcttaa ggcaccttgt taatgcgaaa cgtagctctt
     6721 gttctcttct gcgccattgc ttccgggttt gggcaaggag cgcagcctgc ggccacgccc
     6781 tcgatcgtgt ttgcggtgcg cgatcccgat gcaatcaaag gctacaagac gaatccgcgc
     6841 gttgtgcgtg cgatggtcaa ccggcttgtt ctcgctgcca ctggccagcc cgatgtagcc
     6901 agggcatggg gatcgctggt ttctccgagc gacaaaattg gaatcaagat ctccgcggcc
     6961 ggcggcgaac ttttcactac gcatcatgac gtggtaaacg cgattgtcga cgggctcgcc
     7021 gccgctggtc acccacgcgg tagcattatc gtttgggatc gatctcttgg tggaatgaaa
     7081 ggggccggtt atcgaccgga tattggtggc taccagctca aggcgatcgc gccgcacacc
     7141 ggctatgatc cgaaagcggt tctctctgct cccttgattg gcaaactgat ttggggcgat
     7201 ctcgattatc ggagctacga aggcaaaatg ccgctccttt ccgactccga gaatacgagc
     7261 aacgtcagtc atttttcgaa gatcatttcg actgaagtga cgaagatcat caatgttccg
     7321 gtaatgagca gcagcgaaat gaatggcatc gccggttgca tctacaacgt gaccataccg
     7381 aatatcgaca actggcgtcg ctttgctcaa ggctctcggt ttggagccga aagtctggcc
     7441 gagatttacg gcgatcccgt catcgcgaag aaagtcgttt ttaacttgat ggatggcctg
     7501 gtcgcccaat atgccggcgg tccgcagtcg cagccgaact atgccgtcca tcacgccacg
     7561 ctctatgcga gcaaggatcc agtggcgttg gacgccgtgg cgctgagacg tttagaggaa
     7621 tggcgcgcac gcgcgagttt gcctcctatc gggcgcacgg ctgcttatat cgactttgcc
     7681 acgcagctcg gcctgggtaa ctcggcgcca aatcggatcg aggtgagaaa tatcggccga
     7741 tgaaattccg ccgaaacaag aaaccatgag tgcgcaatca cgtcactctg ttcaggaact
     7801 gcaaacagcg attctgcgct cggtctcgcg ctcgttttat ctttccatcc ggtttttgcc
     7861 ggcgcaatta cgcgaaccgg tcgcagtcgc gtacttgctc gcgcggacga cagacacggt
     7921 tgccgatacg gcacaaatcc ctggaacggt ccgaacggaa actctgaaga tgctctcgaa
     7981 cggaattcag ggcaaagctt cgcgcgaggt ggttatcgat cttatcgcgt catttgtgcc
     8041 cctacaggag aacgcgtccg aacgacggct cctcgaatcc ctgccagatt gtttagaatg
     8101 gctcgagcat gtggaggctg cggatcgcga tgacattcgc gccgtgctcg agaaaatcat
     8161 tcacggccag atgctcgatc tgcagcgctt cgacaatccg acagaaattc gcgcgctcca
     8221 aagtgccgcc gacctggatg aatacaccta tttcgtagcg ggttctgtcg gcgaattctg
     8281 gacgcgcctc tgcttccggc acgtccgaga ttttgccagc ctcaacgaag accagatgct
     8341 tgccctcggg aaacgctatg gcatggcgct tcagttgatt aacgttctgc gcgatgctgg
     8401 ctccgatctc cgggcgggca gatgttattt cccagaatac gaattgggcg cggtccattt
     8461 gagtgctgac gaaatttttt ccgaacagga gcgtttccag ccgatttaca gaagatgggc
     8521 tgataaggcg agagatggac tgaaatgcgg gatgcaatat tcgcgtgcga tcaaaaaccg
     8581 tcgggtgcgc gccgcgacgg ctttgccagc gctgattggg gcacgcacgc ttgcgctgtt
     8641 gcatgacgct ggcgcaacgg ctttgcaacg cacggtgaaa attccgcgct gggaagtgcg
     8701 gcggatgatt tcgtcgctcg ccgttagttt ggcatcgcgc aagcgcatcg acgcaatgtt
     8761 cgaacaatta tccaaatagg gcggttggcc ccgttcagct atcgctcagg gcaagctctc
     8821 aatggctcgc accgcgcgat cccccaaacg ccgaacgata tttctcgagc aggcaggccg
     8881 caccctataa tttgcggccc gggtgcgcgc gttgcttcag aacaaattca gccagctcca
     8941 aatctcgcgg ataagtgatt ttgaaattga agtcgttgtt caggacaaga acaactttgc
     9001 gaccgaggcg ctcgactgcc gaaacttcgt cggtcaatga aacattctcc gtgtaagcgg
     9061 cgcgataagc ttcttcgatc aatccacgct cgaagacttg tggtgtttgc atcacgtaaa
     9121 gttgatgtcg atccaccgag gctgtgacaa taagatctac atcggcgcgt ttgacggtgt
     9181 cgcggattgg ttccgccagt gtagctgccc catagtcgcg gcatttttcg aatacgcgct
     9241 cgatttgttc tgacgtgacg agcggacggg cggcgtcatg cacagcaacg tatttcgcat
     9301 ccgcgctcag acgacccaaa ccgatgcgaa cggaatcctg acgacgttcg ccgcccgtaa
     9361 tgatcgaccg gatctttttg aagtttccaa cgtggctgat ctttctgatc tcgtcctgtc
     9421 gatcttcgcg cgcaacgacg ataatttcgt tgacgcaact ggcgcgctcg aacgcttgga
     9481 gcgtatgcgc gatcactggt ttcccggcga ttggtgcaaa cagtttgtcg aatcccattc
     9541 gttggctgct gccggccgcg acaatgatgg cggttagcat ctgtgggctc aagagggtag
     9601 agaagtcttg ctacagctgc aatccgccgg tcagctttgt catctcctct tgggcgaggg
     9661 ccttgccttg ttcggcggct tgcttgacgg cgctcagcac aatttcctca aggaactcga
     9721 catcattcgg atcgacgact tctttgtcga tcttgatcgc gacgatgtca ccggcgccat
     9781 tggcgattac cttcacttta ccgccgcccg cgttcacttc aacggtcttc ttctcgagct
     9841 cagcctgcgt tttggccatt tgctcctgca tcttctgggc ctgcttcatg agcttgttga
     9901 ggttcataag atttggatca ccctctttgt cattccgagc ggagtcgagg aatctctcat
     9961 tttaaattga gcagtgagag atgtttcgac ttcgcttcgc tggctcaaca tgacaagcaa
    10021 ttcatcctat taggatcagg atttgatttt ggcattgaag atttcaaggg cttcctggat
    10081 gagcggatca tctttgaaat cctcagatcg agaagcctcg acgcctgcag gagtaattgt
    10141 ctgctttgaa ggaaggtcgt catttacggc aagcttcacc gtcaaatctt ttccagtgat
    10201 ttcatacaag agcgtctcga gaactttgcg attcgcctgt gtgcccaaaa tctccatcag
    10261 tggtttctga tccggcgaaa aaccgagctg gaaattgcgt ccttcaattc cgagtacgtg
    10321 tgctgccgct gcagaatttc ggaggaaagc ttttagcgac ggaattttcg tgttgagctg
    10381 ttcccacact tgcgcaggat cgatatcggc
//
LOCUS       DASQUQ010000103         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_292053, whole genome shotgun sequence.
ACCESSION   DASQUQ010000103 DASQUQ010000000
VERSION     DASQUQ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292053"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VE486_02875"
     CDS             complement(<1..218)
                     /locus_tag="VE486_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZA38060.1"
                     /translation="MQSFRFHPVVARWFDQTFGSPTEPQARGWPAIQSGRHVLISAPT
                     GSGKTLAAFLASLDILFREGMQAELPDET"
     gene            complement(331..1128)
                     /locus_tag="VE486_02880"
     CDS             complement(331..1128)
                     /locus_tag="VE486_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HZA38061.1"
                     /translation="MPQAKAVGPDTDGNIAGSNNGEGIGVLVSRTTGVWNTGTGFEAL
                     NHLTSGNQNTATGLPALFSDTNGGFNTATGVFSLYSNTSGFYNNAVGAYALSSNIIGS
                     NNTATGYAALYRNTANNNTDGQLGTASSSRRFKKEIKPIDSASEAILALKPVTFHYKT
                     DKSGTPQFGLIAEEVAEVNPDLVVRDEKGDIYTVRYDAVNAMLLNEFLKEHRKVEDLK
                     KDFQATVAQQQKEIQSLAAQLKAQAAQIPKVSAQLELAKSIPQTVAK"
     gene            complement(1271..2014)
                     /locus_tag="VE486_02885"
     CDS             complement(1271..2014)
                     /locus_tag="VE486_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HZA38062.1"
                     /translation="MLPPFSIVIPCFNEAARIGKTLSATLDYLAAAAPESELIVVNDG
                     STDATATIAREVLSNTKIATRLLENFPNRGKGAAVRSGLLAAQKPIGLFSDADLSTPL
                     EETPKLIEPITDDQIDVAFGSRALDRSLIGIRQPWRREHAGRVFNLLVRVATGLPFWD
                     TQCGFKAFRLDICRPIIEAVRIDGFAFDVELLYLAQRAGLRIREIPVRWNHAEGSKVR
                     FIHDSLRMLREVIALRLQAVAAGADRDVP"
     gene            complement(2169..3257)
                     /locus_tag="VE486_02890"
     CDS             complement(2169..3257)
                     /locus_tag="VE486_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HZA38063.1"
                     /translation="MIENVKLPQAINCFFALILFSAAAPGLAQEPAESVTGSPGKAPE
                     DQLKMQTKKFGLVVHGGAGTIERSKMTPEIEREYRDGLERALTAGYEILKRGGSSLDA
                     TEAAVRVLEDDPHFNAGKGAVFTSAGVNEMDAAIMDGKTLKAGAVASLKHVKNPITLA
                     RLVMDNSPHVMLDCAGAEAFARENGIELVDEKYFFTQQRWDALQKIKEAEKHGGVGGK
                     KFIISEEDRHGTVGAVALDQEGNLAAATSTGGIANKWPGRIGDTPVIGAGTYANNQTC
                     AVSCTGDGEYFIRAAVAHEVSALMEYRGMKLQEAAQTALDAAKKIGGSGGLIAIDKNG
                     EIALPFNTNGMYRGYVDPTGKFVTEIYK"
     gene            3449..>3939
                     /locus_tag="VE486_02895"
     CDS             3449..>3939
                     /locus_tag="VE486_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA38064.1"
                     /translation="MANAHPPVVIAQGIGQIGRNFLREVGSVFWFIAHTFEETFERIQ
                     QGRVPFRAASFFRHTARAGVDSVPLVGLVSFFLGLTMALLTGYQLQRFGTERLIPGLV
                     AISFTRELGPLITGIMLAARIGAAFTAELGTMQVSEEVEAIESMGIGPLRFLAAPRML
                     ALI"
BASE COUNT          799 a         1124 c         1110 g          906 t
ORIGIN      
        1 gtttcatccg gtaattcggc ctgcatgcct tcgcggaaca ggatgtcgag cgacgcaaga
       61 aacgccgcca gcgttttgcc cgaacctgtc ggagcagaaa tcagcacatg ccggccggat
      121 tggattgccg gccagccgcg ggcttgcggt tctgtcggtg agccgaaggt ttggtcgaac
      181 caccgcgcaa caacgggatg aaatcgaaag gactgcatga atacgagaaa acggagtgtc
      241 taatttaggc gacactgcga gaaaaaaaag tcgccgccta cgaatcccga atgcctcgta
      301 gggactcacc gctcacgctt gcgtcaacgg ctatttcgca accgtttgcg ggatagattt
      361 ggctagttcc agctgtgcgc tgaccttcgg gatttgggct gcctgcgctt tgagctgcgc
      421 cgccagggat tgtatttctt tctgctgctg cgcgacggta gcctggaagt ctttctttaa
      481 gtcttccacc ttgcgatgtt ctttgaggaa ctcgttgagc aacatcgcgt ttaccgcatc
      541 gtagcgcacg gtgtagatgt cgcccttctc gtcgcgcacg accaggtcgg ggtttacctc
      601 agccacttcc tcggcgatca agccaaattg cggcgtgccg ctcttgtcag ttttgtagtg
      661 gaaggtcacc ggcttaagcg ccagaatggc ttcgctggcg ctgtctatcg gtttgatttc
      721 tttcttgaaa cgacgcgaag agctggccgt gccgagctgg ccgtccgtat tgttgttggc
      781 cgtgttgcgg taaagcgctg cgtaaccggt ggccgtgtta ttactgccga tgatgttgct
      841 ggacagcgca taagcgccga cggcattgtt ataaaatcca ctggtgttgc tatagagcga
      901 aaaaacgccc gtggcagtgt tgaagccgcc gttggtatca ctaaacagag cgggaagacc
      961 ggtcgccgta ttttggttgc cactggtgag gtggttaagc gcctcgaaac cagtgccggt
     1021 gttccagact ccagtggtgc ggctgacgag cacgccaatg ccttccccgt tgttggagcc
     1081 ggctatgttg ccgtccgtgt cgggacccac cgctttcgcc tgaggcataa agccaaagca
     1141 gaccagcaga aacgggacga gaaatggcgg agttgtcgtt ccaaacttaa tttatgcatt
     1201 catgatggtg tcagtctgga gaaaatctcg ccagcttcgc aagatcaaaa cgacaggcga
     1261 aaatcgatcg tcagggaacg tcgcgatcag cgcccgcggc tacagcctgt aatcgcagcg
     1321 caattacttc ccgcagcatc cgtaagctgt cgtgaatgaa acggaccttg ctcccctccg
     1381 cgtggttcca gcgcactgga atttcacgga tgcgcaatcc tgcgcgctgc gccagataaa
     1441 gcagctcgac atcaaaagcg aaaccgtcga tgcggacggc ttcgataatc gggcggcaaa
     1501 tatcgaggcg gaatgctttg aaaccacatt gcgtgtccca aaaagggaga ccggtggcga
     1561 cgcggaccag caagttgaaa acccggcccg cgtgctcgcg tcgccagggt tgacggatac
     1621 caatcaagct gcgatccaac gcgcgcgaac caaacgcgac gtcgatctga tcatcggtaa
     1681 tcggctcgat taatttcggc gtctcctcca aaggcgtcga gagatcggca tcggaaaaca
     1741 ggccgatcgg tttttgcgct gccagcaatc cagaacgcac ggcggcgcct ttgccgcgat
     1801 ttgggaaatt ctccagaaga cgcgtcgcga tctttgtatt cgacaaaact tcgcgtgcga
     1861 tcgtggcagt cgcatccgtc gatccgtcat tcacgacgat caactcgctc tccggagccg
     1921 cggcggcgag ataatcgagc gtggcactga gcgtcttacc gatgcgcgcc gcttcgttga
     1981 agcaaggaat gacaatggag aacggcggaa gcatgagcaa aaatttgtag aggcgcgtgt
     2041 ccccgcacgc aatcctttgt cattccgagc gaagccgacg aatctcttct tgctaacagt
     2101 aatcccgcat ccgcactagc gggagactaa cgctcgacat gacattcaat acagccgcct
     2161 gtgcacctct atttgtaaat ttcggtaaca aacttgccgg ttggatcgac gtagccgcgg
     2221 tacatcccgt ttgtattaaa tggcaacgct atctcgccat ttttgtcgat tgcgatcaag
     2281 ccgccgctgc caccaatctt tttagctgcg tccagagccg tctgcgccgc ttcctgtaat
     2341 ttcatcccgc gatattccat cagcgctgag acttcatgcg ccacggcggc acgaataaaa
     2401 tactcgccat cgccggtgca cgaaacggcg caggtttgat tgttggcgta agtgccggcc
     2461 ccgatgaccg gcgtatcgcc gattcgccca ggccatttgt ttgctatccc acctgtggaa
     2521 gttgctgcgg ccaaattccc ctcctgatcg agcgcgacgg cgccgaccgt gccatgtcga
     2581 tcctcctcgg agataataaa tttctttccg cccacgccgc catgcttttc ggcctctttg
     2641 attttctgta aagcatccca gcgttgctgg gtaaaaaaat acttctcatc gacaagctcg
     2701 attccgtttt ccctggcaaa tgcttctgcg cccgcgcaat cgagcatgac atgcggcgag
     2761 ttgtccatca ccagtcgcgc cagagtgatg ggatttttca cgtgcttcaa actggcgacg
     2821 gctccagctt tcagcgtctt accgtccatg atcgctgcgt ccatttcgtt cacgccggcg
     2881 cttgtaaaga cagcgccttt tcccgcgtta aaatgcggat catcctcgag cacgcgcacc
     2941 gcagcttcag tggcatcgag gctggatcca ccgcgcttca ggatttcgta acccgccgtc
     3001 agcgcgcgct ccaggccgtc gcgatattcg cgctctattt ctggcgtcat cttgctgcgc
     3061 tcaattgttc cagcgccgcc gtgaaccacg agaccgaatt tctttgtttg catttttaac
     3121 tgatcctccg gggctttccc cggtgagccc gtaacggact ccgccggttc ctgagcgagg
     3181 ccgggtgccg ctgcactaaa gagtatcaag gcaaagaagc aattgatcgc ctgcggcagt
     3241 tttacattct caatcatgag ttggcgaccc tagcggccgg aaggcggcgc cggttgctgc
     3301 gcaggaggag gcgaaggcag cgcgcggttt cacagaggcg aatgtaaacg gttcgtccgg
     3361 gtgatgtcga aggctttcat gcaggcgtaa tgtgttgctc aactttgtcc ttttccgcat
     3421 cgcgaagtga ggcaacttcc ccctcgacat ggcaaacgct catccgcccg ttgtgatcgc
     3481 ccagggaatt ggtcagattg gacgcaattt tctccgcgaa gtgggcagcg tgttctggtt
     3541 catcgcccat acgttcgagg aaacgttcga gcggattcag caagggcgtg tgccatttcg
     3601 cgccgccagt tttttccgtc acacggcgcg cgctggtgtc gattccgtgc cgctggtggg
     3661 actggtttcg tttttcctcg gtctgaccat ggcgctcctg accggctacc agttacagcg
     3721 gttcggaacg gagcggttga tccccggcct ggtcgcaatc agcttcaccc gcgagcttgg
     3781 cccgctgatc accggcatca tgctggcagc acggatcggt gcggcgttta cggcggaatt
     3841 gggcaccatg caggtttccg aagaggtcga agcgatcgag tcgatgggca tcggaccgtt
     3901 gcgtttcctg gcggcgccgc gaatgctcgc gcttatttt
//
LOCUS       DASQUQ010000104         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_293105, whole genome shotgun sequence.
ACCESSION   DASQUQ010000104 DASQUQ010000000
VERSION     DASQUQ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293105"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VE486_02900"
     CDS             complement(<1..1061)
                     /locus_tag="VE486_02900"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZA38065.1"
                     /translation="MTATSDSQRMEKVVSLCKRRGFIFQSSEIYGGLNGAWDYGPLGV
                     ELKRNLKNYWWHVMVHERDDVVGMDGSILMNRAVWKASGHEDTFSDPMVDCRTCKARL
                     RADQMIEKKGVRRCPNCGGKDLTEARPFNLMFKTYVGATEDESSVTYLRPETAQAIFV
                     QFKNVLEVSRKKLPFGIAQIGKAFRNEINPRNFTFRSREFEQMELEYFCRAEEGMKLL
                     DYWLEERLKFYESIGILREKLHARNVPDEERAFYSKGTYDIEYDFPFGRQELEGVAYR
                     TDYDLQQHIQASGKSLDYFDEETKHRFVPHVVEPSAGVDRTVLALICEAYSEDEAPDE
                     KGKMETRIVLRFHPRMAPI"
     gene            complement(1183..1635)
                     /locus_tag="VE486_02905"
     CDS             complement(1183..1635)
                     /locus_tag="VE486_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZA38066.1"
                     /translation="MSEHKVTLSWQRGDKPFEYQKYSRDHTWKFDGGHEMQASAAPAY
                     LGNPKLVDPEEAFVASLSSCQMLTFLAVAAKKKFVLDEYIDEAVGHMEKNVEGRLAIT
                     RVKLRPRLKFSGDNQPTSEEIKEMSHIAHEQCFIANSVKTEITVEPQS"
     gene            1801..1872
                     /locus_tag="VE486_02910"
     tRNA            1801..1872
                     /locus_tag="VE486_02910"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1833..1835,aa:Val,seq:cac)
     gene            1986..>2279
                     /locus_tag="VE486_02915"
     CDS             1986..>2279
                     /locus_tag="VE486_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38067.1"
                     /translation="MISNRRRSGWGECKGAAATLFILLLTPVLARAESIGAWAKTRIE
                     IKAADKAAKALVNPNVTAKDRLHVEQIYYQLVRDNPQSVPAQNAFAAFLWKSGK"
BASE COUNT          488 a          624 c          598 g          569 t
ORIGIN      
        1 ttgatcggcg ccatgcgtgg atggaagcgc aagacaattc gcgtctccat tttccctttt
       61 tcgtccggcg cttcgtcttc cgaatatgct tcgcagatta gtgccaaaac tgttcgatct
      121 acacccgcgc tcggctcgac cacgtgaggg acaaagcgat gtttcgtttc ctcatcgaaa
      181 taatcgagcg atttgccgct ggcttggatg tgttgttgca aatcgtagtc ggttcgataa
      241 gcgacccctt ccagctcctg ccggccgaac ggaaaatcgt attcgatgtc gtaggtgcct
      301 tttgaataaa aagcgcgttc ttcgtcagga acgtttcgtg cgtgcaactt ttcgcgcagg
      361 atgccgatgc tctcatagaa cttgagccgc tcttccaacc agtaatcgag cagcttcatt
      421 ccctcctctg cccggcaaaa atattcgagc tccatctgct cgaattcccg ggagcgaaag
      481 gtgaaattgc gcgggttgat ttcgttgcga aatgctttgc caatctgggc gatgccaaac
      541 ggcaatttct tgcgcgagac ttcgagcacg tttttgaatt gaacaaaaat ggcctgtgcg
      601 gtctcagggc gaagataggt gacgctggat tcgtcttccg tcgcgcccac gtaggtcttg
      661 aacatcagat tgaacgggcg cgcttcggtt agatctttac cgccgcagtt tggacatcgc
      721 cggacgcctt ttttctcgat catttggtcg gcgcgtaaac gcgctttgca cgttcgacaa
      781 tcgaccatcg ggtcactgaa cgtgtcttca tggccgctcg ccttccaaac cgcgcggttc
      841 atcaagatcg agccgtccat accgacgacg tcgtcgcgct cgtgcaccat tacatgccac
      901 caataattct tgagattccg ctttagttcg acgccaagcg ggccgtaatc ccaggcgccg
      961 ttcaagccgc cgtagatttc gctcgactga aaaatgaaac cgcgccgctt gcataagctg
     1021 acgacttttt ccatgcgctg ggaatcggat gttgcggtca tcggaacgga gttaaccgcg
     1081 gattgcgcgg atttcacgga tggaaataaa gcagtatttt cttgggtcca acgggcggca
     1141 cgctcgcctc cacagcatct gcgtcatccg cgttgtccgt ggttaacttt gcggctcgac
     1201 tgtgatctcg gtcttgactg aattggcgat gaaacattgc tcgtgcgcga tgtgactcat
     1261 ttccttgatc tcttccgacg ttggctgatt atcgccggag aatttgagcc ggggtcttaa
     1321 tttcacgcgg gtgatggcca gcctgccttc cacattcttt tccatatggc cgacggcctc
     1381 atcgatatac tcgtcgagca cgaacttctt tttggcggcc accgcaagga aggtgagcat
     1441 ttggcagctt gagagcgatg caacgaatgc ttcctctgga tcaacaagtt tcggattccc
     1501 tagataggca ggagcagctg atgcctgcat ttcgtgtccg ccatcgaatt tccaggtgtg
     1561 atcgcgcgaa tatttctgat actcgaacgg cttgtcgccg cgttgccagc tgagagtgac
     1621 tttgtgttcg gacatccttc tgtataaaat gcgatgcgtt gcagggcaag cgctcccgct
     1681 atcctcagcg gaacattgga caagcaatcc gccctgcgcc cactgcgctg agacagcgca
     1741 cgctacagct ccgcttgcgt caggtctctc gcgctctatt ttgtcggctt ctgaaaactt
     1801 gcgcgattag ctcagtggta gagcgcttgc ttcacacgca agaagtcgca ggttcgaacc
     1861 ctgcatcgcg cacgatctgc ccgttctgga ctttttggga tagaggcgaa ctttgatgga
     1921 ctaaaagttt catttccagc gcttaataaa gtgaagatga tctctccagg aaagcgacag
     1981 caacgatgat cagcaaccga aggcggagcg gctggggtga atgcaagggg gctgccgcca
     2041 cgctctttat tctgctcctt acacctgtgc tcgcgcgtgc ggaatccatc ggtgcatggg
     2101 caaaaacgcg gatagaaatc aaagcagccg ataaggcagc caaggcgctc gtcaatccca
     2161 acgtaacggc aaaagatcgt ctgcatgtgg agcaaatcta ctaccaactc gtccgggaca
     2221 atccgcaatc tgtccccgcc caaaatgcct ttgccgcatt tctctggaaa agcgggaag
//
LOCUS       DASQUQ010000105         7729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1099897, whole genome shotgun sequence.
ACCESSION   DASQUQ010000105 DASQUQ010000000
VERSION     DASQUQ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099897"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VE486_02920"
     CDS             <1..437
                     /locus_tag="VE486_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38068.1"
                     /translation="GDYGKQPPSMRTAAKMLWLMSSNPVLRQKDAPTLAKTDEVLDKF
                     VDGIVKADDANDVLYALEASGDYDPGPNLEKIRAPLLAINSADDLINPPELGILEREI
                     KRVPHGRAIVIPPSDQTRGHGSHTIAALWKTKLEALLKEPAK"
     gene            520..897
                     /locus_tag="VE486_02925"
     CDS             520..897
                     /locus_tag="VE486_02925"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZA38069.1"
                     /translation="MDPHRNEEPAFLFKTLADPTRLRLLNLLACGETCVCELTDTLRV
                     VQPKVSRHLALLKRAGLVEAHRDGKWMHYRWARHGDPLVRHVLAGLHQWMAKDDRMNG
                     ERRRRNKVCCEISRRKTKRTKTK"
     gene            894..1364
                     /locus_tag="VE486_02930"
     CDS             894..1364
                     /locus_tag="VE486_02930"
                     /inference="COORDINATES: protein motif:HMM:NF040413.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HZA38070.1"
                     /translation="MKTNEFISGLRVAPGNQLIFVDLAGHAVHGGYHLTELKAALFET
                     VDCGGQTNRWPETIVQLWVPDDPDDEYVTAAKFLKIFDKVRGMIPLNFDAEIRVEYGD
                     EHFFPSTYQVRSVTHDREATRVLLEPPETTCKARDRRTAAAHSSSPSDSCCLAA"
     gene            1540..3240
                     /locus_tag="VE486_02935"
     CDS             1540..3240
                     /locus_tag="VE486_02935"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZA38071.1"
                     /translation="MRRNFFCFGFFLTIVFAVPAQNRSPSPTVTDFDIIIKGGTVYDG
                     SGVEPKRVDVAIRGDRIAGVGDFKTVTAKTVIDANGLAVAPGFINMLSWSTVSLIQDG
                     RSQSEIRQGVTTEIMGEGESMGPVNERVREHMLRNQGDIKYDIKWNTLAEYLQYLEKR
                     GVSCNVASFIGATTIREYVIGFENKAPTPEQLDQMRELVRKEMEAGALGIGTSLIYPP
                     AFYAKTEELIELCKVAAKYQGKYISHMRSEGNRLLEALDELIRIGREAKIPAEVYHIK
                     AAGQQNWPKIDDLLSRIEGAQKEGLKITADMYAYTAAGTGLDACLPPWTEDGGYPALF
                     KRLRDPATREKIAAEVSKDSDEWENLYIGAGSPDRILLVGFKSEKLKPLTGKSLAEVA
                     KMRGKDPITTIMDLIAEDQSRIESIYFLMSEENVKKELAKPWISFGSDEASQAPEGVF
                     LKSNPHPRAYGNFARVLGKYVRDEKVIPLKEAIRRLSGLPATNLGLDHRGFIKERMFA
                     DVVVFDPATISDRATFEKPHQYAVGVKHVFVNGVQVLKDGDHTGAKPGRALWGPGKVH
                     "
     gene            3337..3744
                     /locus_tag="VE486_02940"
     CDS             3337..3744
                     /locus_tag="VE486_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZA38072.1"
                     /translation="MDPNRSIKLRWLHGTLHDVTSELARLQFSRFASQTWQPAINAYR
                     CENCIRVCVDLAGVERSRIDLTVEPERLIVRGVRDVPEPTHAEGRAVQLLAMEIDYGP
                     FEREVRLPALVDIEKVHAEQRNGLLWISLPLKS"
     gene            3741..6188
                     /gene="lon"
                     /locus_tag="VE486_02945"
     CDS             3741..6188
                     /gene="lon"
                     /locus_tag="VE486_02945"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZA38073.1"
                     /translation="MNEEPLVALQEGSDGKPAPGNLGGMGNVADTSAPQVPDVLSILP
                     LRSFVVFPGTILPLTVGRAASIKLLDETLPQTKIIGLLTQRDETKDDPEPQDLYPVGT
                     AAIVLKLLRQSDNHVIIVVQGLRRFALRKVVATDPYLRAEVELLNSKAPPRTKEWEAT
                     FRNLRDSAAKLLELTPDVPEQARAAVLSIEDPEQLADFLAPNLNVETAQKQTILEELD
                     VEKRLAAVQTSISAQLEIAQIQQKLQQDVQSQFSDAQRRAYLREQMKAIQRELGEEAG
                     ATDQIERLRKRLEEAKPPEDVLAQADRELKRLEVIPSASPEYSVIVSYVETIAELPWS
                     KLSQDNLDLDQAQKILDRDHYDLEKVKRRLIEYLAVRKLNPTGHGPILCFLGPPGVGK
                     TSLGQSIADALGRKFVRMSLGGIRDEAEIRGHRRTYIASMPGRIIQELRRAGTRNPVM
                     MLDEIDKIGTDFRGDPASALLEVLDPRQNNTFSDRYLDVPFDLSQVLFIATANYMDGV
                     PEPLRDRMEVISLPGYTEREKLEIAKRYLVRRQLEENGLKPEQCEWQEEAFRRIINDY
                     THEAGVRELERQIGAVCRAVAAQVARGKAERVTITPERVAELLGPAKYVRETKLKTSQ
                     PGVATGLAYTPAGGEVLHIEATRYPGKGSVTLTGHIGEVMKESVQAALSLVRSRNNQI
                     GAKPEDFRNIDVHVHVPAGAVPKDGPSAGIAMFTALASLFSNTPVRPDVAMTGEITLR
                     GLVLPIGGLKEKSLAAMRAGISTVIIPKLNEKDLVDVPEEAKQKLKFVPVENVDEALS
                     IALEKDAAKPTSESKVA"
     gene            complement(6229..>7729)
                     /locus_tag="VE486_02950"
     CDS             complement(6229..>7729)
                     /locus_tag="VE486_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38074.1"
                     /translation="WKSFNLSQLTLSQAPSVIIRPTWIAIVGSPYVGPLGLAGALVAG
                     IYFRRLDPFLRVLAIAFGVISLYGLLSGFGTNLGFAYVNFHLPLINRIREAGRHLVLF
                     VIGISFLSALGYSLLARSLEQYKQSRNARLLILPAALMLIFGGIVLWELFQNGGSPIQ
                     TGFWVLALAPILFVLGRFWRLSGYKNLVSAAVLVSLAAVVIPVRGFSLSQSDFNKPMN
                     LLSHKVIQSFADKIDASGYRVDFRDNDTFPNRFWAMNASYYGIKSFYNQLTPQPYDQF
                     RFSLMGNVPHLREMMGARYVLCGPDNSPSDEGARQILETEGYRLYENPNPMGRLTLLH
                     RIAGVVESEKEFLRKIRSGFDYLSEAYVDGDRFEEVRQSLGSSQMVPSAQDRIEKIVD
                     QPNRSYSAIESGSASLLILNEWFTPAWKVRVNSKNQPVLRVNEWQTGVLLPAGKNRVE
                     FEYRPTLFRVLMALNRIAIVLLLLFGIYAVVRKARDRSRKGQSPDQVCS"
BASE COUNT         1872 a         2144 c         2182 g         1531 t
ORIGIN      
        1 gcggcgatta tggaaagcag ccacccagca tgcgcaccgc agcgaaaatg ctttggctca
       61 tgagcagcaa tccggttctg cgacagaaag atgcgccaac actggcgaaa acggacgagg
      121 tgctcgacaa attcgtcgat ggaatcgtga aagcggacga cgcaaatgac gtgctttacg
      181 cgctggaagc gtcaggcgac tacgatccag ggccgaactt ggaaaaaatc cgcgcaccgc
      241 ttctcgcgat taattccgcc gacgatctga tcaatcctcc cgaacttgga attctcgaac
      301 gcgagatcaa acgtgtccca cacggccggg cgatcgtcat tccgccgagc gaccaaacgc
      361 ggggtcacgg cagtcacacc atcgcggcgt tgtggaaaac taaactcgag gcgctgttaa
      421 aagagccggc taaataactc caaaaattaa tctggcggtg acaaagaatc tcggtgacgg
      481 ctattgacac atatatgttt atccgtatat gtgtgccaaa tggacccgca cagaaacgag
      541 gagccggcat ttctcttcaa aacgttggct gatccaacgc ggttgcgttt gctcaatttg
      601 ctcgcgtgcg gagagacgtg cgtttgcgaa ttaacggaca cgcttcgcgt ggtccagccg
      661 aaagtgtccc ggcacctcgc tctcctgaag cgcgcaggcc tggtggaagc gcaccgcgat
      721 gggaaatgga tgcattatcg ctgggcgcgt catggtgatc cgctggtccg ccacgtccta
      781 gccggcttgc accaatggat ggcgaaagac gaccgcatga atggcgagcg tcgcagacgc
      841 aacaaagtct gctgcgagat cagccgaaga aaaaccaaga gaacaaaaac caaatgaaaa
      901 caaacgaatt catctccgga ttgcgcgtcg cgccgggtaa tcagttgatc tttgtcgacc
      961 tcgccggtca cgccgtgcac ggcggttatc atcttacgga attaaaagcg gcgttgttcg
     1021 aaacggttga ttgcggcggg caaacgaacc gctggccgga aacgatcgtg caactctggg
     1081 tgccggacga tccggacgat gaatacgtga cagccgccaa attcctgaag attttcgata
     1141 aagtgcgcgg catgattcca ctgaatttcg acgccgaaat ccgcgttgag tacggggatg
     1201 aacatttctt tccgtccaca tatcaggttc gttcggtgac gcatgatcgt gaggcaacgc
     1261 gagttttgct ggagccgccc gagaccacgt gcaaggcacg cgaccgtcgc acggcggctg
     1321 cacattcgtc gagcccgagc gattcatgct gcctggccgc ataatcctgc tgctaaagac
     1381 gcagccccag tgctgtagcg tgcgctgtcc tcagtgcact tggtcttttg gcggcacagc
     1441 gggatctgct tttggagtgc ggcgacgtgt cgccgcactc caaactggtg tcggcttttt
     1501 gcatcgacat cgtggctcta cacttcttga atcgacgcca tgaggcggaa tttcttttgc
     1561 ttcggctttt tcctgacaat cgtgtttgca gtgccggcgc agaatcgatc tccatcgcca
     1621 accgtcacgg acttcgacat catcatcaaa ggcggcacgg tttatgacgg cagcggcgtc
     1681 gagccgaaac gggtcgatgt tgcgatccgg ggcgatcgga tcgccggcgt cggcgatttc
     1741 aagacggtga cggccaaaac cgttatcgat gcaaacgggc tcgcggtcgc gccgggattc
     1801 atcaacatgc tttcgtggtc aacggtgtcg ttgattcagg acggccgttc gcagagcgaa
     1861 atccggcagg gcgtcacgac cgagatcatg ggcgaaggcg aatcgatggg cccggtgaac
     1921 gagcgggtgc gcgaacacat gctgcgcaac cagggagaca tcaaatacga catcaaatgg
     1981 aatacgcttg cggagtattt gcagtatctc gaaaaacgcg gcgtgtcgtg caatgtggcc
     2041 tcgttcatcg gcgcgaccac gatccgtgag tacgtgatcg gctttgaaaa caaagccccg
     2101 actccggaac agctcgatca gatgcgcgaa ctcgtgcgca aagagatgga agcgggcgcg
     2161 ctcgggattg gcacgtcgct gatttatccg ccggcgtttt atgcgaagac ggaagagttg
     2221 atcgagctgt gcaaagtcgc cgcgaaatac cagggcaaat atatttcgca catgcgcagc
     2281 gaggggaacc ggctgctcga agcgctggat gaattgatcc ggatcggccg ggaagcgaaa
     2341 attcccgctg aggtttacca catcaaagca gctgggcagc agaattggcc gaagatcgac
     2401 gatttgcttt cacggattga gggcgcgcaa aaagagggac tgaagatcac cgcggacatg
     2461 tacgcgtaca ccgctgcggg aaccgggctc gacgcttgtt tgccgccctg gaccgaggac
     2521 ggcggttatc ccgccttgtt caagcgcctg cgcgacccgg cgacgcgaga gaaaatcgcg
     2581 gcggaagtca gcaaagacag cgacgagtgg gaaaatcttt atattggtgc aggttcgccc
     2641 gacagaatct tgctcgtcgg attcaaatcc gaaaaactga aaccgctcac aggaaaatcg
     2701 ctcgccgagg tggcaaagat gcggggcaag gatccgatta cgaccatcat ggacctgatc
     2761 gcagaagatc aatcccggat tgagtcgatt tatttcctga tgtcggagga gaacgtcaaa
     2821 aaagaattgg cgaagccatg gatttccttc ggctctgatg aagcgtcgca agctccggag
     2881 ggcgtgttcc tcaaatcgaa cccgcatccg cgcgcatacg gaaatttcgc acgcgtcctc
     2941 ggcaaatacg tgcgggatga aaaagtcatc ccgctgaaag aagccattcg caggttgagc
     3001 ggattgcctg cgacaaatct cggcctggat caccgcggtt tcattaaaga acgaatgttc
     3061 gccgatgtcg ttgtgttcga cccggcgacg atttccgatc gcgccacatt cgagaaaccg
     3121 catcaatacg ccgttggtgt aaagcacgtt ttcgttaacg gcgtacaggt gctcaaagat
     3181 ggcgaccaca ccggcgccaa gccgggtcgc gccttgtggg gcccgggcaa agtccactaa
     3241 tcgactcgcg ccgcgtcagc gcgccggtga tcagtaagaa tccgtaattg cagttccggt
     3301 tgaaaacgga gcgcaccggc tgaggtttac cttgagatgg atccaaaccg cagcattaaa
     3361 ctgcgctggc tgcacggcac gctccacgat gtgacgtctg agctggcgcg tttgcagttt
     3421 tcgcgctttg cctcgcaaac gtggcaaccg gcgatcaacg cctatcgctg cgagaattgt
     3481 attcgtgttt gtgtcgatct tgccggagtt gaacggtcgc gcatcgacct cacggtagag
     3541 ccggaacgcc tcattgttcg tggcgttcgc gatgtccccg agccgacgca tgcggaaggc
     3601 cgcgctgtac agttgctcgc catggagatt gattatgggc cgttcgaacg cgaggtgcgt
     3661 ttaccggcgt tagtggatat tgaaaaagta catgctgagc agcgcaacgg gctcctctgg
     3721 atttcgctgc cattgaaatc atgaacgaag aaccgcttgt cgccttgcaa gaaggcagtg
     3781 atggaaaacc cgctccggga aatttaggcg gaatgggaaa cgtcgccgac acttcggcgc
     3841 cacaggttcc cgacgtgctt tccattctgc cgttgcgcag ctttgttgtt ttcccgggga
     3901 cgatcctccc gctaacggtt ggccgcgccg catcaatcaa gctgctcgac gaaacgctgc
     3961 cgcaaaccaa gatcatcggt ttactgacgc aacgcgatga aactaaagat gatccggagc
     4021 cacaagatct ctacccagtc ggcaccgcgg cgatcgttct aaaacttttg cgccaatccg
     4081 acaatcacgt gatcattgtg gtgcaagggc tccgccggtt tgcgctgcga aaggtcgtcg
     4141 ccaccgatcc gtacctgcgg gctgaagtcg agctgctcaa ttccaaggcg ccaccgcgta
     4201 ccaaggaatg ggaagcgact ttccggaatt tgcgcgattc ggccgcgaaa cttctcgagc
     4261 tcacccccga tgtgcccgaa caagcgcgcg cagccgttct cagcatcgag gaccccgaac
     4321 aactggccga tttcctcgcg ccgaatttga atgtcgagac cgcgcaaaaa cagacgattc
     4381 tcgaggagct cgatgtcgag aagcgactgg ccgccgtaca aacgagtatc tcagcgcaac
     4441 tggagatcgc acaaattcaa cagaagctgc aacaagatgt gcagtcgcaa ttttccgatg
     4501 cgcaacggcg cgcttatttg cgcgagcaaa tgaaagccat ccagcgcgag ctgggagaag
     4561 aagccggggc gaccgaccag atcgagcgac tgcgcaagcg cttggaagag gcaaaaccgc
     4621 ctgaggatgt cctggcacaa gccgatcggg aattgaagcg tctcgaggtc attccatccg
     4681 ccagccccga gtattccgtg atcgtgagct atgtggagac gatcgcggag ctgccgtggt
     4741 cgaaactcag ccaggacaat ctcgatctcg atcaggcaca aaaaattttg gatcgcgacc
     4801 attacgattt ggaaaaagta aagcgacgac tgatcgaata tcttgccgtg cgcaaattga
     4861 acccaacggg acacggtccc attctttgct ttcttggccc tcccggtgtt ggcaagacca
     4921 gcctcggtca atcgatcgcc gacgcgcttg gtcgaaaatt tgtgcggatg agtctgggtg
     4981 gtatccggga cgaggcggag attcgcgggc atcgccgcac gtatattgct tcgatgccgg
     5041 gccgcatcat tcaagagctg cgccgggcgg ggacgcgcaa tcccgtaatg atgctcgacg
     5101 agatcgataa gatcggcacc gattttcgcg gtgatccggc cagcgcgctg ctcgaagtgc
     5161 tcgatccccg ccagaacaat acattttccg atcgctacct cgatgtgcct ttcgatctgt
     5221 cgcaggtgct cttcatcgcg accgcgaatt acatggatgg tgtgccggaa ccgttgcgcg
     5281 accgcatgga agtgatttcg ctgcccggat acaccgagcg agagaaactg gagattgcaa
     5341 aacgatatct ggttcggcgg cagctcgagg aaaacggtct caaaccagag caatgcgagt
     5401 ggcaggagga agccttccgc cggattatca acgattacac gcatgaggcc ggcgttcgcg
     5461 aattggaacg ccaaatcgga gcggtctgtc gtgcagtggc agcccaggtt gcccgcggaa
     5521 aagctgaacg cgtcacgatt acgccggaac gggtggccga attactgggg ccagccaagt
     5581 acgtgcgcga aacgaaactc aaaaccagtc aaccaggcgt cgccaccggt ctggcctata
     5641 cgcctgccgg gggcgaagtg ctacatatcg aagcaacccg atatccgggg aagggcagtg
     5701 tcacgctcac cggacacatc ggcgaagtga tgaaagaatc ggtccaggcc gcgctcagtc
     5761 tcgtgcgcag ccgcaacaat cagatcggcg ccaaaccgga ggatttccgc aacatcgatg
     5821 tgcacgtgca cgttccagcc ggcgcggtgc caaaagacgg cccgtcagcg ggcatcgcca
     5881 tgttcaccgc gctcgcttcg ctcttcagca acacgccggt ccgccccgac gtcgccatga
     5941 ccggtgaaat cacattgcgc gggctcgtgc tgccgatcgg cgggctgaag gaaaaaagtc
     6001 tggccgccat gcgcgctggc atttccacag tgatcatccc gaagctcaac gaaaaagatc
     6061 ttgtcgatgt tcccgaggaa gcgaaacaaa aactcaaatt cgttccagtc gaaaatgttg
     6121 atgaggcctt gagcatcgcg ctggagaagg acgccgccaa gcccacatcg gaatccaagg
     6181 tcgcgtagtt aactcgtctc aaacttttcg gaaggtcggt agcgcggatc agctgcaaac
     6241 ctgatcggga gattgtccct tcctactcct atcccgcgcc tttcggacaa ccgcataaat
     6301 cccaaaaagc agcagcagta cgatggcgat tcgattcaat gccatcagca cgcggaacag
     6361 cgtgggacga tactcaaact cgacacgatt ttttccggcc ggcaggagca cgccagtttg
     6421 ccactcgttt acacgtagaa ccggctgatt tttgctattc acccggacct tccaggcggg
     6481 cgtaaaccat tcgttcaaaa tgaggagcga cgccgaacca gactcgatcg cggaataact
     6541 acggttcggt tgatcaacga tcttttcgat gcggtcctga gcagacggaa ccatctgcga
     6601 actgcccaaa gactgacgaa cctcctcgaa ccggtcaccg tccacgtagg cttcggagag
     6661 ataatcaaag ccgctcctaa tcttccttag gaattctttt tcgctctcga cgacaccggc
     6721 aatgcgatgc agcaaagtca accggcccat cggattcgga ttttcgtaca ggcggtaacc
     6781 ttccgtttcc agtatctgcc ttgcgccctc gtcagaaggc gaattgtcag ggccgcataa
     6841 cacataacgc gcgcccatca tctcgcgaag atgcggaaca ttccccatca ggctgaaccg
     6901 aaactgatca tacggttgtg gagtcagctg attgtaaaaa ctctttatcc cataataact
     6961 tgcgttcatc gcccaaaatc gattgggaaa cgtgtcatta tcgcggaaat ccacccgata
     7021 gccgctggca tcgatcttat ccgcgaagct ctggattact ttgtgactaa gtaaattcat
     7081 gggcttgttg aaatctgact gcgaaagtga aaaccctctt accggaataa ccacagccgc
     7141 cagcgaaacg agcacggcgg ctgatacaag gtttttgtag cccgataatc tccagaaacg
     7201 acccaggacg aaaaggatcg gcgctaacgc cagaacccag aatccagtct ggattgggct
     7261 tccgccgttc tgaaaaagct cccatagaac aattccgcca aaaattagca tcaacgctgc
     7321 cgggagaata agcagacgcg cattacggct ctgtttgtat tgctcgaggc ttcgcgccag
     7381 aaggctgtag ccaagcgcgc ttaggaagga gataccgatt acgaacaaga caaggtgccg
     7441 cccagcttcc cgaatccgat taattaacgg gaggtgaaag ttgacatagg cgaatcccag
     7501 gttggttcca aaaccagaga gcagtccata taggctaatc actccaaagg cgatcgccag
     7561 cacgcgcaaa aacgggtcaa gccggcggaa gtagatccca gccaccagcg ctccggccag
     7621 accaagcggg ccgacgtacg gacttccgac gatggcaatc caagtcggtc tgatgataac
     7681 actcggtgcc tggctcaatg taagttgaga aaggttgaag cttttccat
//
LOCUS       DASQUQ010000106         9070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_561122, whole genome shotgun sequence.
ACCESSION   DASQUQ010000106 DASQUQ010000000
VERSION     DASQUQ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561122"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2167
                     /locus_tag="VE486_02955"
     CDS             <1..2167
                     /locus_tag="VE486_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZA38075.1"
                     /translation="QTPDGIRDLQSIAVPLLLNLEPPAFEETISVLQPQKLRERFHRF
                     REQIEAGSPRAQFQLEFDPLGLIGPALKPFAESNQIEEEQPLASSDRTMRVFLVVTNQ
                     PSISPFECQRVMRQVDAFRAHARDGWEGGPLDVLVTGRSAYVAEISLSMRHDIIATVI
                     GSVVLVGIIFFAGFGRWMPLLGMGFSLLLSCLVALALGLLIFGRLNMVTIGFCAILVG
                     LGVDFAILIFGRYQQARTDGEPHPQAISTAIQKLGRAVFFGALTTAAGFLALILSGSI
                     GFSQLGVLIAIGILFAGLFMCTILFLFVGEGQPAARRDWIFEAVKKYVARSVRRPAPI
                     LILATVILGTLTVFGFSPKPPLPFDASGRSLQPKKSHAGHALAAIMSNMPRRWEPVLA
                     IMRAPDAQRLHDDWKKIGAHWSELQAAGKIKSFSTPAALAPSPRSIQHNRERLRKIDF
                     QSVRRTLGETLDAEGFSPDSFSSAYKLLDDLQSVADPNAALPNWRDKLPKSSSWWFLV
                     DRYFAQDPLLTVGFVTSNQPVKDHAQSVALSRELPVPGVAMILSGWSYTLANLLPWAE
                     RQLLIVTGAMAVFEGLLLAVLYRDWRLWVIQEITLVFGLGAMLATMKLFNLPLNLLNV
                     LAFRLVIAIGVDYGIYVLFAWQKAREIEHDIAGVVKPILLAGLTALAGFGSLVLAKNS
                     SLSGLGVACAIGIFWNLVATIFFTLPAAAAARPKTWREE"
     gene            2232..2870
                     /locus_tag="VE486_02960"
     CDS             2232..2870
                     /locus_tag="VE486_02960"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HZA38076.1"
                     /translation="MAALFLFIIASMARAEALSEAELKNLLTRIRENRITQADFQEQR
                     IIRLMQKPIVNSGQVWFQPPNKFRREAKGNSPSLTVSNGQQLWIYYPNFRSAERYSLG
                     KRTPLDSIIASINAALNLENIENTFHITATRAGNEYELELTPRTAAMKRAFQKLNLRI
                     NDKLQAERTDMLQPNGDRIVTIYSNQSREPVPPSTFEFTPPPGTDVTSPLGK"
     gene            complement(3256..4614)
                     /locus_tag="VE486_02965"
     CDS             complement(3256..4614)
                     /locus_tag="VE486_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZA38077.1"
                     /translation="MEWLYIIAASLFIFGLKMLSSPQTARRGNVVSAVGMLLAIVATL
                     SKGGLSFAWIAAGIVIGSAIGAVSAYRVKMTAMPEMVAIFNGFGGVASLLVGWAEYHM
                     HRDAPTFTIVAIYLAVLIGGITFTGSVLAFAKLAELVTGRPVMFRGQHIVNGLLLAAA
                     VLSGITFTLAPEASYAILILGVVLALVIGIFAVLPIGGADMPVVICLLNSYSGLAAAA
                     AGFVLANNMLIVAGSLVGASGIILTRIMCKAMNRSLANVLFSGFGAAAGTIAGAGAKG
                     KAKSISPHDAYFALEAARSVVFVPGYGMAVAQAQHVVKELADLLEANGTEVKYAIHPV
                     AGRMPGHMNVLLAEADVPYDQLVEMDAINPLMPTIDVALVIGANDVVNPAAREDKSSP
                     IYGMPIINVDQAQNVFALKRSMASGFAGVENPLFFKDNTTMIFGDAKKTLQEIVTEFK
                     AA"
     gene            complement(4621..4899)
                     /locus_tag="VE486_02970"
     CDS             complement(4621..4899)
                     /locus_tag="VE486_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZA38078.1"
                     /translation="MIPVLVFIFIIAAFLGFELISKVPSQLHTPLMSGSNAISGITIV
                     GAIVATGVAHGEFATALGFLAVIFAMINVVGGYLVTDRMLAMFRKKKK"
     gene            complement(4896..6032)
                     /locus_tag="VE486_02975"
     CDS             complement(4896..6032)
                     /locus_tag="VE486_02975"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZA38079.1"
                     /translation="MRIFVPKETAPGEERAPVTPETAKKLVALGAEVAVETGIGASIH
                     RSDTEYKSTGASVLDDRMAGLREADIVLRLRKPPIEEAAALREGCIHISFLDPFNERD
                     LIEQFAAAKVSAISMEMIPRTSIAQKMDALSSQANLAGYLAVVLAAEELDRIFPMMTT
                     PAGTIQPARVFVIGVGVAGLQAIATAKRLGARVEAFDTRPAVEEQVRSLGARFVKVDL
                     GKTGETEQGYAKPLTEEQLAAQREVMAKHCASSDVVITTAQVFGRKAPVIVTTAMVQR
                     MRPGSVVVDLAIETGGNVECAEFDKVVEVNGVRVFGYANMPSRAAIPASEMYSNNIGS
                     LVEHLWNKERSGIVIDPADEFLRGCLITHEGQVVNQIIKKHHNL"
     gene            6154..7065
                     /locus_tag="VE486_02980"
     CDS             6154..7065
                     /locus_tag="VE486_02980"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZA38080.1"
                     /translation="MTDYVDAFTKQICEVIVGKNQAVKLAVACLLARGHLLIEDIPGV
                     GKTTLSQALARSLALAFQRIQFTSDLLPADILGNSIFDRDEQRFVFHRGPLFSQVVLI
                     DEVNRATAKTQSALLEAMEEHQISIDGVTYELPEPFFVIATQNPQHQVGTFPLPESQL
                     DRFLMRIELGVPDRASERAMLLGMERRQMLKELKPVFNPQTLLEIQDSVRKVHASSAL
                     LDYLQDLLDASRQRHSTGLSPRAGLALLHASQAWALMNGRDMVLPEDIQAVGIAVMAH
                     RLGHDIEQPGQSGRTLADNLLHSVPVP"
     gene            7062..7949
                     /locus_tag="VE486_02985"
     CDS             7062..7949
                     /locus_tag="VE486_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZA38081.1"
                     /translation="MKERKFPSVYPTISFGGLLFVLAAMWYAASSQNSAAAYLLLFAL
                     TAVFLVSIPHTLINLAGMTITLESVKPAFAGQEISLPIEIMNGSRATRHGISVAPSGS
                     RRERKRVDRISAGKAARVTLRFPAPQRGEHKIGTLCLTSVYPLGFVRVLKQFATSQSY
                     VVYPKPAGDLQLPLSRASSLHGGSQADIGEGDDFAGVRAYVPGEPQRHIDWKAVAREQ
                     PLMTKQFTAEAQGVVYLDFSAMRFTDVEARLSQLALWVIEAERARRPYGLRLPGTEIP
                     PAVGESHFHQCLRVLSRFQ"
     gene            7946..>9070
                     /locus_tag="VE486_02990"
     CDS             7946..>9070
                     /locus_tag="VE486_02990"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HZA38082.1"
                     /translation="MKTPTDTSIPRQPLLWLAAALLFTLPPMFASLATWVPSFFFLTL
                     AMKFWMEPKGYRLRSPIWKLVLAAVALAAIFFSYGSLKGIESGVSVLVVLMSLKILEA
                     HTAREFQVMVMMGWVLCLCGFFLSQDFAVALCLLAAFALLLVALVQFHSGPSIGSFWS
                     PVGATCKLLAQAAPLVVLLFVLFPRINTGLRFEIDLHSAATGFSDRLSPGSIAALTNS
                     PDIAFRAEFPDRKTRPPGPMYWRGAVMWHCEGMEWRAPYAPAPSSVSSTSSPGDKAIR
                     QRITIAPHRGRWMFALDRPFEVPPKTMLARGNYLWSVQPIRKARRYEVVSSPKLTENE
                     LTPRERKEALEVPASVTPAVRELAESWTAQNSNPRATVNSA"
BASE COUNT         1901 a         2661 c         2480 g         2028 t
ORIGIN      
        1 gcagacgccg gatggtattc gcgatctgca atcaatcgca gtgcctctgc tcttgaatct
       61 cgagccgcct gcattcgaag aaacgatatc ggtcctgcag ccgcaaaaac ttcgcgagcg
      121 attccaccga ttccgcgagc aaatcgaagc gggctcaccg cgagcgcagt tccagctcga
      181 gtttgacccg ctgggcctaa tcgggcctgc gctcaaacct ttcgccgaga gcaatcagat
      241 cgaggaggaa cagcccctgg cttcatcgga tcgtacgatg cgcgtttttc tcgtcgtcac
      301 gaaccagcct tccatcagcc cgtttgagtg ccagcgggtg atgcgccagg tagatgcgtt
      361 tcgcgcgcac gcgcgagatg gctgggaggg cggacctttg gatgtgctcg tcacggggcg
      421 ctcggcctac gtcgccgaaa tttcgttgag catgcgccat gacattatcg cgaccgtgat
      481 cgggtcggtc gtgctggtcg gcatcatttt tttcgccggc ttcggtcgct ggatgccgct
      541 gcttggaatg ggattttcgc ttttgttgtc gtgtctcgtc gcgctcgcgc tcggcctgct
      601 gattttcggc cggctcaaca tggtgacgat tggattttgc gccatcctcg tcgggctcgg
      661 agtcgatttc gcaattctga tctttggccg ctaccagcag gcgcgcacgg acggcgaacc
      721 gcatcctcag gcaatctcga ccgcgattca aaagttgggg cgcgccgttt tcttcggcgc
      781 cctcacaacg gcagcgggct ttctcgccct gattctgagc ggctcgatcg gcttttctca
      841 actcggcgtg ctcattgcca tcggcattct ctttgccggt ctattcatgt gcacgattct
      901 gtttttgttc gtgggcgaag gacagccggc agctcgccgc gattggatct tcgaagccgt
      961 gaaaaaatac gtggcccgaa gcgtgcgacg tcccgcaccg atcttgattc tggcgacggt
     1021 aatcttaggg accttgacgg tctttggttt ctcgccgaaa ccgccgcttc cttttgatgc
     1081 gagcggacgt tcgctccaac cgaaaaagag ccatgccggt cacgcgctgg ccgcgattat
     1141 gagcaatatg ccaaggcgct gggaaccggt gctggccatc atgcgcgcac ctgacgcgca
     1201 gcggctccac gacgattgga aaaaaatcgg ggcgcattgg agcgagctgc aagcggcggg
     1261 caagatcaaa agtttctcaa cgcccgcggc tctggcacct tccccaaggt cgatccaaca
     1321 caatcgagaa cgattgcgca agatcgactt ccaaagcgtc cgccgaacat tgggcgaaac
     1381 gctcgatgcc gaaggattca gtcccgattc gttttccagc gcatacaagt tgctcgacga
     1441 tttacagtcg gtggccgacc caaacgcggc gttaccgaac tggcgagaca aacttccaaa
     1501 atcttcgagc tggtggttcc tggtcgatcg ctacttcgcg caagatccct tgctcaccgt
     1561 cggttttgtt accagtaacc aaccagtcaa agaccacgcg cagtccgttg cgcttagccg
     1621 cgaattgccg gtgccgggcg tggcgatgat cctttcagga tggagctata cgctcgcgaa
     1681 tttgctgcct tgggcggaac gtcagttgct catcgttacc ggcgcaatgg ctgtgttcga
     1741 ggggctgctg ctggcggttt tgtatcgcga ttggcggctt tgggtcatcc aggagatcac
     1801 gctggtcttc ggcctcggcg cgatgcttgc gacgatgaaa ctattcaacc tgcccttgaa
     1861 tttgttaaat gttctggcct ttcggctggt gatcgcgatc ggggtcgatt acggcattta
     1921 cgtgctgttc gcgtggcaaa aggcgcgaga gatcgagcac gacatcgccg gtgtcgtgaa
     1981 gccaatcttg ctcgcgggtc taaccgccct tgccggattt ggatcgttgg tgctcgccaa
     2041 aaattcttcg ctcagcggcc tcggcgtggc ctgtgcaatc ggcattttct ggaacctggt
     2101 cgcgacgatt ttcttcaccc ttccagcggc ggcggccgcc agaccaaaga cttggcgcga
     2161 agagtaacgt ggataagatc ggcaatcaga tgaaccagat gatccaacaa atagcgcgcc
     2221 gacaaatttg tttggctgcc ctctttcttt tcattatcgc atccatggcc agggcggagg
     2281 cgctttccga ggccgagctc aagaatctcc tcacccgaat tcgcgagaac cggattaccc
     2341 aggccgattt tcaggagcaa cggattattc gtctgatgca aaaaccgatc gtgaattcag
     2401 ggcaggtctg gttccagccg ccaaacaaat tccggcgtga agcgaaaggg aactcgccca
     2461 gccttaccgt gagcaatggt cagcagcttt ggatttacta tcccaatttc aggtcggccg
     2521 aacgttattc gttgggaaaa cgcaccccgc tcgattcaat cattgcctca atcaacgctg
     2581 ccttgaacct ggagaatatc gaaaatactt tccacatcac tgcgacacgt gccgggaacg
     2641 aatatgaatt ggagctgacg ccgcgaacag ccgcgatgaa acgggcgttt caaaaactca
     2701 atctgcgaat taatgacaaa ttgcaggctg aacgcacgga catgttgcag ccaaacgggg
     2761 atcgaattgt aaccatctac tcgaaccagt ctcgcgagcc ggttcctcca tcaacgttcg
     2821 aatttacacc gccgcccggc accgatgtca ccagcccgct gggaaaataa cggccgataa
     2881 cctctgagaa ttgaaaaggc gctggcgttc caaattcgat caggccaggc agagattccg
     2941 cctcactccc acggaaaagc gagtagtggt tttcattctc gcggcgttcg ttctcggcct
     3001 gggcacaaag tgttaccggg acgcccatcc gtcgccttcc aaatcagctg cgcatcacga
     3061 aaaagtcgga ccatagattt gtagtggctt gttgaaaagg tttccgatcg tggcacagcc
     3121 atcttggctg tggggccgac ggcgcatctt gtccgccagg cgaacggact accccctggc
     3181 gagcctgtcg gctttaaact ggcaggcagg gatctctgtg ccaccggtta ccggcaccaa
     3241 aaccaacaca aaaaactacg ccgccttaaa ttcggtgacg atttcttgca gggtcttctt
     3301 cgcgtcgccg aagatcatgg tcgtgttgtc tttgaaaaag agcgggtttt ctacgccggc
     3361 aaaaccggac gccatggagc gcttgagcgc gaagacgttc tgcgcttgat cgacattaat
     3421 gatgggcatg ccgtaaatcg ggcttgattt gtcttcgcgc gcggcgggat tgaccacgtc
     3481 atttgcgccg atcacgagcg ctacgtcaat tgtcggcatc aaaggattga tcgcgtccat
     3541 ctcgacgagt tgatcgtaag gaacgtccgc ttcggcgagc aagacgttca tgtgcccggg
     3601 cattcggccg gctaccgggt gtatcgcata ttttacttcg gtgccgtttg cttcgaggag
     3661 atcagccagt tccttaacca catgctgggc ttgggcgacg gccattccgt aaccgggcac
     3721 aaacacaact gaacgcgccg cctcgagcgc aaaataagca tcatgcggcg agatcgattt
     3781 ggctttgcct tttgcgcccg cgccggcaat ggtcccggca gctgctccga aaccgctgaa
     3841 gagaacattt gcaagtgagc ggttcatcgc tttgcacatg attcgggtaa ggatgattcc
     3901 gctcgccccg acgagtgacc ccgcgacgat gagcatgttg ttggcaagaa caaaaccagc
     3961 agcagcggcc gcgagaccgg aataactgtt cagcaaacaa atgacgactg gcatgtccgc
     4021 gccaccgatc gggaggacag cgaaaatgcc aatcaccagc gcgaggacga ctccgaggat
     4081 caagatcgcg tagctcgcct cgggcgcgag cgtaaaggtg atgccggaca aaacagcagc
     4141 cgcaagcaac agtccgttaa cgatgtgttg cccgcgaaac atgaccggcc ttcccgtaac
     4201 cagctcggcg agtttcgcga acgccagaac acttcccgtg aacgtgattc cgccgattaa
     4261 gacagcgaga taaatcgcga caatcgtaaa cgtcggcgcg tcgcggtgca tgtggtattc
     4321 cgcccagccg accagcagac tcgccacgcc gccgaaacca ttgaagatcg ccaccatctc
     4381 cggcatggcc gtcatcttca ctcgataagc cgagacggcg ccgatcgcag aaccgattac
     4441 aatccccgcc gcaatccatg caaaacttag cccccctttc gacagcgtcg cgacaatcgc
     4501 gagcagcatt cctactgccg aaacgacatt gccgcgtcgc gcggtctgcg gcgagctgag
     4561 cattttcagc ccgaagataa aaagtgacgc cgcgatgatg tagagccact ccataccgtt
     4621 ttactttttc ttcttcctga acattgccag catccggtcg gtcaccagat aaccgccgac
     4681 cacgttgatc atcgcgaaga tcacggcgag aaatccgagc gcggtggcga actcaccgtg
     4741 ggcgacgccc gtcgccacaa ttgccccgac gatggtgatc ccagagatcg cattggatcc
     4801 cgacatcagc ggcgtatgca actgcgacgg cactttcgag atcaattcga atccaaggaa
     4861 cgcggcaatg ataaaaataa agacgaggac agggatcata agttgtggtg ttttttaatg
     4921 atttgattca cgacttgacc ctcatgggtg atgaggcagc cgcggagaaa ttcgtccgcc
     4981 ggatcaataa ctatgccgct gcgttctttg ttccagaggt gttcgaccag actgccgatg
     5041 ttgttcgaat acatttcgct cgccggtatc gccgctcgtg aaggcatgtt ggcgtagcca
     5101 aacacgcgaa ccccgtttac ctccacgacc ttgtcgaact cggcgcattc gacgtttccg
     5161 cccgtttcga ttgcaagatc gacaacgacg ctgccgggtc tcatccgttg caccatcgct
     5221 gtggtcacaa tgaccggtgc ctttcggcca aaaacctggg ccgttgtaat gacgacatcg
     5281 gacgaggcgc aatgttttgc catcacctca cgttgcgctg ccagttgctc ctcggttagc
     5341 ggtttggcgt agccctgctc ggtctccccc gtctttccaa gatcgacctt cacgaaccgc
     5401 gcaccgagcg agcgcacttg ctcctccacg gctgggcgcg tatcgaacgc ttctacgcgt
     5461 gcgccaagtc gttttgcggt cgcaattgcc tgcaacccgg caacgcccac gcctatcaca
     5521 aaaacacgtg ccggctggat cgtgccggcc ggcgtcgtca tcatcggaaa aatccgatcc
     5581 aactcttccg cggcgaggac cacagcgaga taaccggcga gattcgcctg agaactgagc
     5641 gcgtccatct tttgcgcgat gctcgtccgc ggaatcatct ccatgctaat cgcgctcacc
     5701 ttcgccgcag cgaactgctc gatcagatcg cgctcgttga aaggatcgag aaaactgatg
     5761 tgaatacagc cctcccgcag agctgccgct tcctcgatcg gcggctttcg caaacgcagc
     5821 acaatgtctg cttcgcgcag cccagccatc cgatcatcaa gcacagaagc tcccgtgctt
     5881 ttgtattccg tatcgctgcg gtgaattgac gcgccgatgc cggtctcaac tgcgacttca
     5941 gctccgagcg ccaccagttt tttcgccgtc tctggcgtaa ctggcgcgcg ctcttcgccc
     6001 ggagccgttt ctttaggaac aaaaatgcgc atcgcctatt tcttggcaat gcgcgccaac
     6061 aaaagcaagt aacagattcg agaccggggt ggccgcgcgc gtttccgctt atcccggttt
     6121 gcgaagtaca tcgttcccac agatgatccc ggagtgaccg attacgttga cgccttcacg
     6181 aagcagattt gtgaagttat tgtaggcaag aaccaggcgg taaaactcgc agtcgcctgt
     6241 ctgctggccc gcggccacct cttgattgaa gatattccgg gcgtaggcaa gacaaccctc
     6301 tcccaggcat tggcgcgctc gctcgcgctc gcctttcagc gcatccaatt tacgagcgac
     6361 ctgcttccgg ctgatattct cggcaattcc atctttgatc gggatgagca gcgttttgtg
     6421 tttcatcgcg gaccgctctt ctcgcaagtt gtgctgatcg acgaagtcaa tcgcgcgacc
     6481 gccaagacgc aaagcgcgct gcttgaagca atggaggaac accagatctc gattgatggc
     6541 gtgacctacg agttacccga accgtttttt gtaatcgcaa cccagaatcc gcaacatcaa
     6601 gtcggtactt ttccgctgcc cgaatctcag ctcgatcgct ttctgatgcg gatcgaactg
     6661 ggcgtgccgg atcgcgccag cgaacgggcc atgcttctgg gaatggagcg ccgccagatg
     6721 ctcaaggaat tgaagccggt gtttaatcca caaacattgc tcgaaattca agatagcgtc
     6781 cggaaggtac acgcttcgtc tgcgctgctc gattacctgc aggatttgct cgatgcctcg
     6841 cggcaacgtc acagtaccgg cttgtccccg cgtgcgggtc tggctttgtt gcatgcatcc
     6901 caggcttggg cgctgatgaa cggacgcgac atggtcctgc ccgaagatat tcaagccgtg
     6961 ggcatcgcag tcatggcaca tcggctggga catgacatcg agcagcccgg acaaagtggg
     7021 cgtacactcg ctgacaatct gctacacagc gtgccggtcc cgtgaaagaa agaaagttcc
     7081 cttccgtcta tccgaccata agttttggcg gtctgctgtt tgttctggcc gcgatgtggt
     7141 acgcggcctc aagtcaaaat agtgcagcag cgtacctgct cttgttcgcg ctgacggcgg
     7201 tctttttagt ttcgattcca cacaccttga tcaacctcgc aggcatgaca ataacgctcg
     7261 aatccgtgaa accggcgttc gcaggtcagg agatttcact gccgatagag atcatgaacg
     7321 gatcccgcgc gacacggcat ggaatcagcg tggctccatc gggctcaagg cgagagcgca
     7381 aacgtgtaga tcgcatttca gccggcaaag ccgcgcgtgt gacgcttcga tttcccgcac
     7441 cacagcgggg cgaacataag ataggaacgt tgtgtctaac gagtgtttat ccgctcggtt
     7501 ttgtccgcgt cttaaaacaa tttgcgacct cccaaagcta tgtggtgtat cccaagcctg
     7561 cgggagatct gcagctcccg ctaagccgcg cgagttcact ccatggcggg tcacaagcgg
     7621 acattggaga aggcgatgac tttgccggag tgcgcgccta tgtgccgggc gagccgcaac
     7681 gtcacattga ttggaaagcc gtggcacgcg agcagccgct tatgacaaaa cagtttacag
     7741 ccgaagcgca gggggtggtg tatctcgatt tttctgccat gcgttttacc gatgtggaag
     7801 ccaggctatc gcaactcgcg ctttgggtaa tcgaagccga acgcgcccgg cgcccatacg
     7861 gccttcgcct gccaggtacg gagattccgc cggcagttgg cgaatcacat tttcaccagt
     7921 gcctgcgcgt actcagccgg ttccaatgaa gacgccgacg gatacaagta ttccccgaca
     7981 gccgctgctt tggctggcgg ccgcattgct ctttacgctg ccgccaatgt tcgcctctct
     8041 cgcgacctgg gttccaagtt tctttttcct cacactcgca atgaaatttt ggatggaacc
     8101 aaaagggtat cggcttcgtt ctccaatttg gaaactggtt ctggcggcag tcgcgctcgc
     8161 cgccatcttc tttagctacg gttccctcaa gggcattgag tcgggcgtgt ccgttctcgt
     8221 cgtcctgatg tcactcaaga ttctggaagc gcacaccgcg cgtgagttcc aagtaatggt
     8281 catgatggga tgggtgcttt gtctttgcgg gtttttcctt tcccaggact tcgcagtcgc
     8341 actttgtctc ctggcggcat tcgcattgct gcttgtcgcg ctcgtccagt ttcacagcgg
     8401 accgtcaatc gggtctttct ggtcgccggt aggtgcgact tgcaaactcc tcgctcaggc
     8461 ggcgcccctc gtcgtcttac tgtttgtttt gtttccccga ataaataccg gactccggtt
     8521 tgaaatcgat ctccactcgg ccgctaccgg tttttccgat cgcctttctc cgggcagcat
     8581 tgcagctctt actaattctc cggacatcgc tttccgcgcg gagtttcctg accgcaaaac
     8641 tcgaccgcct gggcccatgt attggcgcgg agcagtcatg tggcattgcg aagggatgga
     8701 atggcgcgct ccatatgctc ctgcaccaag ctctgtttcc tctacgtcat cgccgggcga
     8761 caaggcaatt cgacagcgga ttacaatcgc accgcatcgt ggccgctgga tgttcgcgct
     8821 cgatcgaccg tttgaagttc ctcctaagac gatgcttgca cgtggcaatt acctgtggag
     8881 cgttcagccg attcgaaaag cgcgccggta tgaggtcgtt tcatctccga aactcacaga
     8941 aaacgagctc acaccgcgtg aacgtaagga agcgctcgag gtgccggcat cggttacgcc
     9001 ggctgtgcgc gaactagcag aatcctggac cgcgcaaaat tccaatccgc gcgctacagt
     9061 aaacagcgcg
//
LOCUS       DASQUQ010000107        13641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1023271, whole genome shotgun sequence.
ACCESSION   DASQUQ010000107 DASQUQ010000000
VERSION     DASQUQ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 13641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13641
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023271"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VE486_02995"
     CDS             complement(<1..815)
                     /locus_tag="VE486_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZA38083.1"
                     /translation="MNARTDVDPVALEVELRKNIRGEVRFGDGDRALYSTDSSNYRQI
                     PIGVVIPHDRSDVITTVAICRKFGAPITCRGGGTSLAGQCCNVAVIIDFTKYMNRVIE
                     IDAAQKLARVEPGLILDELQKKLNKHGLIFGPDPATHNYCAIGGMLGNNSCGVHSVMA
                     EFYGGGARTSDNVRELEVLLYDGTVLRVGKTGDGELDGILREGGRRSEIYRKLVDLRE
                     RYGELVRQRFPKIPRRVSGYNLDDLLPENGFNVGRALVGTEGTCAIVLEATLE"
     gene            complement(848..1450)
                     /locus_tag="VE486_03000"
     CDS             complement(848..1450)
                     /locus_tag="VE486_03000"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HZA38084.1"
                     /translation="MKQPRSRHRQDGADFSIEKYGEKTQEKEECDEQLLAEYAVMLAL
                     YLASVAALTGVALERGKLPRKFGMLDLAALGTATHKLSRIVAKDRITSILRAPFVNYI
                     RSAGAGEVEEEPRGRGIQRGIGQLISCPYCMAPWCATALGFGLVLAPRVTRFFTAILG
                     SVAISDFLHRAYLKTKEEGEQRKSSPGASLPGRDRDRTER"
     gene            complement(1455..2627)
                     /locus_tag="VE486_03005"
     CDS             complement(1455..2627)
                     /locus_tag="VE486_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZA38085.1"
                     /translation="MERTIEGQTKKLAAHSLSDGLPQLRDPYQFGKINELQVSAFTIP
                     TETPEADGTYEWDSTTIVTVEVTGGGKRGLGYSYADTATAKLIEEKLKDVVVGCDAMS
                     VTSAWMSMVHSIRNLGRPGICSMAVSAVDTALWDLKARLLELPLVTLFGQVRDAMPIY
                     GSGGFTNYSNDHLQKQLRGWVEQGIQRVKIKIGRDAAIDRQRVAIARAAIGEDVELFV
                     DANGAYSRKQALAQAQAFAEFNVTWFEEPVSSDDLEGLRLLRDRAPAGMEIAAGEYGY
                     DIDYFRRMIEAGAVDVLQADATRCGGFTGFLQAATLCDAHHLALSSHCAPALHLHIGC
                     ATPALRYAEYFHDHVRIERMIFDCVVEPTDGTLRPDLGQPGLGLTLKRADAQKFAI"
     gene            complement(2711..4501)
                     /locus_tag="VE486_03010"
     CDS             complement(2711..4501)
                     /locus_tag="VE486_03010"
                     /inference="COORDINATES: protein motif:HMM:NF006129.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HZA38086.1"
                     /translation="MADTASDFLIKRITEWGLKRIYGYPGDGINGIIGAIDRADGAIE
                     YVQVRHEEMAAFMACAHAKFTGEVGICLATSGPGAIHLLNGLYDAKMDHAPVVAIVGQ
                     QARAAIGGDYQQEVDLVTLFKDVAHEYVHMASTPAAIRHLIDRAIRIAKAERTVTCII
                     IPNDVQEMPAVETPPRKHGTIHSGADYASPRVVPRDVDLRRAAEVLNAGQKVAMLVGA
                     GALQATDEIIETADILGAGVAKALLGKAVIPDDLPFCTGPIGLLGSKPSWDMMEECDT
                     LLMVGSSFPYSEFLPKEGQARGVQIDIDPKLVSVRYPMEVNLVGDSAETLRALIPLLE
                     RKQERQWREHIENNVAEWWQILEARAMNEAKPINPQRVFWELSSRLPDNCIIVADSGS
                     AANWFARDLKVRRGMMASLSGNLATMGPGVPYAIAAKFAFPDRVVIATVGDGAMLMNG
                     INEVVTIAENWKQWRDPRLVILVLANRDLNQVTWEQRVMAGDPKYQVSQTVPQFEFAR
                     YAEMLGLSGIRIDKLEQIAPAWEQALSASRPVILEAITDPEVPTLPPHITFEQAKKFT
                     ESMIKGEPHLGHMIKQTFKEAIEGFLPHKK"
     gene            4723..5160
                     /locus_tag="VE486_03015"
     CDS             4723..5160
                     /locus_tag="VE486_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38087.1"
                     /translation="MGDPRNIAHAKKVVQEKHHKKKDSPKARAAKPQPNREQQTDDKQ
                     SAGGESSAPKAEAAKPDQPPDSTQLEKSLAVLSTLKDLEFHSRANIEKLAELSLTIEE
                     ELKQKAFAETIGAVYSAQDAFQSKILKLIEDYEAECTRLKPAG"
     gene            complement(5210..6547)
                     /gene="chrA"
                     /locus_tag="VE486_03020"
     CDS             complement(5210..6547)
                     /gene="chrA"
                     /locus_tag="VE486_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HZA38088.1"
                     /translation="MKLTPLEQTVARQDRHLPPSFAEAFRFWLKLGFISFGGPSGQIA
                     MMHAELVEKKKWIDEARFLHALNFCMLLPGPEAQQLAIYIGWLLHKTRGGIIAGVLFV
                     IPSIFILWALSYIYIAYGSIPWIAAILYGLKPAVLAIVAAAVIRIGQRALRNVIMWLV
                     AAAAFVAIYFFRVPFPMIIISAGVLGFIAGKISPVLIPAIVKTNAGSTIDREGALPIQ
                     GRPTLGRCLRICAISLLLWWLPIALAGIFLGTDHAIFREGIFFSKAALVTFGGAYAVL
                     PYVSQQAVENYHWLGAGQMLDGLGLAETTPGPLIMVLQFVGFLGGWNHPGGLSRFLAA
                     TLGALITTWATFVPCFLWILLGAPYVERLRGIRSLSSALSTVTAAVVGVILNLAIWFG
                     LHVIFPATGHVDWFAVMICGIAFFGMLRWKWNIVPVVLGSGLLGLIYTLAGFR"
     gene            complement(6544..7479)
                     /locus_tag="VE486_03025"
     CDS             complement(6544..7479)
                     /locus_tag="VE486_03025"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HZA38089.1"
                     /translation="MRSAVKKKLTWLLLLFRLPATQKAERVAMWRKLKKSGAIQITTS
                     TYLLPDEPARYECFQWLTKEIRDAGGDATLVRAREIEGLPNDKLVDLFNAARDKEYGD
                     LKKAVQSFIARQKKSDAELVMAEIARLTRQFRELREIDFFDSARGHEVAMLLRRAEGP
                     RRSGKLQVLDARQYRGKTWLTRPRPEIDRVGSAWLISKFIDPKARFVFAPTAQAVPEA
                     IPFDMLDAEFSHHGNCCTFETLTKRFAISDRSVAKIGEMIHDADLDDGRFQRVEGVGI
                     DRVFKGWAKEGVPDDEILRRGFECFAALYAFLQRR"
     gene            7522..8247
                     /gene="pepE"
                     /locus_tag="VE486_03030"
     CDS             7522..8247
                     /gene="pepE"
                     /locus_tag="VE486_03030"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HZA38090.1"
                     /translation="MKSGPRILLISNSTVYGRGYLDHVEAQIKTFLNDVRNVLFFPFA
                     LYGRDDYAAKAKARFASMGYSVESAHGMRDPHKALEQADAIFIGGGNTFRLLKALEDL
                     DLIEPIRRKVSSGAPYIGSSAGSNVAGPTIKTTKDMPIVQPRSFDSLGLVPFQISPHY
                     QDPDPASTHMGETQEERILQFLEENETPVVGIRESAWLVCERGAVTLKGETGARIFRR
                     GEAPAEAKPGRNIVDLVGGPDGH"
     gene            8237..9244
                     /locus_tag="VE486_03035"
     CDS             8237..9244
                     /locus_tag="VE486_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZA38091.1"
                     /translation="MDTESTLLLSRGDVDQLLNLSDCIDAVEKVFRQQHDLALPGLLG
                     IKTQTGGLHVKTAMLSAERHYIVAKLNTNFPGNREHFGLPTIQGLLLLYDADSGRVLA
                     VIDSIDITIKRTAAATAVAAKYLARKDSSVATICGCGQQSRAQLRALLLILPLTTICA
                     FDIDERAAIAFAAELSRELKIDIELMRNLPEAIRNSDVCVTCTTAPKFFVRQEDVASG
                     TFIAAVGADDVHKQEIDPALIASAKVVADSLQQCCAIGDTHHAIEKALMRRENVYAEL
                     SEIVSGAKPGRINEDEIIIFDSTGVAIEDAAAATLVYEKARSTAIRNQFDFAAFAANA
                     R"
     gene            9263..10408
                     /locus_tag="VE486_03040"
     CDS             9263..10408
                     /locus_tag="VE486_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZA38092.1"
                     /translation="MRTKPLTRSSVLDVLRQLIAVPSVNPTLAPHEGQGEGNIATFVN
                     NWFASKGVRSWSDEVAPKRFNAVAQLGEGQGAALVFCAHLDTVSTDGMSIPPFEARCE
                     GNRVYGRGSYDMKGGAAAIMSAMAALALDPPRGKVFAALVADEEYASIGAQHFVAHYK
                     ADACVLTEPSEGSLILCHKGFVWAEIETRGRAAHGSRWDLGVSAIGKMGKIVTALEDF
                     DRQQLRRRVHPQVGPASQHSSLIAGGSGLSTYAESCTLKVERRTLPGETPEQVIRELH
                     EVVSSTGEKAKIRLLLDRPPLTCSRDANIASCLRTAVTSTLGKPPPEAGVSYWMDAAL
                     FAAAGMDTIDYGPTGGGAHEAVEWVDLDSVVNCAAILTETAGLFCNE"
     gene            10401..11294
                     /locus_tag="VE486_03045"
     CDS             10401..11294
                     /locus_tag="VE486_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZA38093.1"
                     /translation="MNKCPHPATRSWIAGAQFSARVTEAQSFLSAYFAPTPLIAARRI
                     SAATGCDVYLKLESSLPTGSFKPRGALFAIYSQLKEKPIREVVTASTGNHGAAVAYAA
                     RQFGLSATIFLPFNSNRQKRSKIVKFGAAIREAGKDLAEAAVAAAAYAATEDAYLLDD
                     ASDPAVPIAAATIACEIFDQLPTVQKIYVPVGDSALIRGIASVEKRRPLPPQLIGVQA
                     ERAPSYYDSWKAAAVRPSDLCDTIADGLATRRPLASNVAAMCKLVDDFVLVSEEALIN
                     SFGTRRKSLASQQAQHPQQRC"
     gene            11404..11904
                     /locus_tag="VE486_03050"
     CDS             11404..11904
                     /locus_tag="VE486_03050"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HZA38094.1"
                     /translation="MKWITRENIKVDRVACPWLIRHFVDADAEFVFLPADTDWSKIDN
                     GAVFNVPKCELGHHGEDVSFNSILEKYRLGDPAMALLGEIVRAADSHPTNPHPAGEGL
                     RWIAHGFSTADFTDHEILERKFVVYDALYRESKRQITKCAKAAEQAPRTKTALPSLFE
                     IQSTVR"
     gene            11931..12557
                     /locus_tag="VE486_03055"
     CDS             11931..12557
                     /locus_tag="VE486_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZA38095.1"
                     /translation="MFGIHDFGLFLAAGILLNLTPGPDTAYILGRSIVQGRDAGIASA
                     LGICVGSNFHTCAAALGLSAILATSAVAFSTIKLLGGAYLIFLGIKMLLHLRKELSLP
                     SNFRLRTTAAAFRQGVFTNVLNPKVGLFFLAFLPQFIDPASNTKVLAFLALGLTFVTT
                     GTIWCLILAWFASAFSERLRANETIGQWLDRAAGALFIFLGLRLATAK"
     gene            12684..12998
                     /locus_tag="VE486_03060"
     CDS             12684..12998
                     /locus_tag="VE486_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38096.1"
                     /translation="MNTPKLFTIAVVSAGLAFGVVAQAGEKKHEEQEISSSDVPAAVQ
                     QAADSHAKGGKIVRWEKEGADYEAVIEKQGKEWGFKFDANGKMLGKHDESKEKGEKGE
                     KY"
     gene            13310..>13641
                     /locus_tag="VE486_03065"
     CDS             13310..>13641
                     /locus_tag="VE486_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HZA38097.1"
                     /translation="MTTRTRWPLIACVALATIASSPAADLDTAKIDQITGLKGKLNEK
                     EGVYKVTFPRNDVKIVVDGWTMPPFMGLGTWAAFTKGAHSEAMVMGDTVLFEDEVNAT
                     MTAALDNGL"
BASE COUNT         3140 a         3876 c         3735 g         2890 t
ORIGIN      
        1 aattccaacg tcgcctcaag aacgattgcg caggtgcctt ccgttccgac gagagcgcgc
       61 ccgacattga agccgttttc gggcaggaga tcgtcgagat tatatccgga aacgcgacgc
      121 ggaatcttgg gaaaccgctg ccggacgagc tcgccgtatc tttctcgaag atcgacgagt
      181 ttcctgtaaa tttcgctgcg ccgcccgcct tcgcgtagaa tcccatcaag ctcgccatcg
      241 cccgttttcc caacacgcaa aaccgttccg tcatagagca gcacttccaa ctcgcggaca
      301 ttgtcggacg ttcgcgcgcc gcccccgtaa aattcagcca taacggaatg cacgccgcac
      361 gagttattac cgagcatccc accgatcgcg cagtaattgt gcgtagctgg gtccggacca
      421 aagatcagcc cgtgtttgtt caattttttc tgtagctcat cgaggatcaa tcccggttcg
      481 acacgtgcca gtttctgcgc tgcgtcgatc tcgatgacgc gattcatgta tttcgtgaaa
      541 tcgataatca cagcaacgtt gcagcattga ccggccaggc tcgtcccgcc gccacgacaa
      601 gtgatcggtg ctccaaattt tctacagata gcgactgtgg ttatcacgtc gctgcgatca
      661 tggggaatca ctacgccgat cggaatctgg cggtaattgg agctatccgt ggaataaagc
      721 gcgcggtctc cgtcgccaaa gcgcacttct ccgcgaatat tctttcgcaa ctccacctcc
      781 aacgccacag gatcgacatc tgtccgcgcg ttcattccgc tcgagaggtg ccgtgacctt
      841 aacctgctca gcgctccgtc cggtcgcgat ctcggccagg gagcgaagcg ccaggtgagg
      901 atttcctctg ttctccttcc tccttcgtct tgaggtaagc gcgatgtaaa aaatccgaga
      961 tggccacgct tcccaagatt gcagtgaaga atcgcgtcac gcgcggcgcc aaaaccagac
     1021 caaatccgag cgcggtcgcg caccacggcg ccatgcaata cgggcaggaa atcaattgcc
     1081 cgatgccgcg ctgaatacct cgtcctcgtg gttcctcttc cacttcaccc gcgccagcgc
     1141 ttcgtatgta attcacaaat ggcgcgcgca aaatgctcgt gatcctatct ttcgcgacga
     1201 tacggctcaa tttatgggta gcggtcccga gcgctgcgag atcgagcata ccgaatttcc
     1261 ttggtaattt accccgctcc agggcaactc ctgtcaatgc ggcaacgctg gccaggtaaa
     1321 gggccagcat caccgcatat tcggcgagca gctgctcgtc acattcttct ttctcttgcg
     1381 ttttctcgcc gtatttctca atggaaaagt cagcaccgtc ctgacggtgc cgggatcgtg
     1441 gctgcttcac ataattaaat cgcaaacttc tgcgcatctg cgcgctttag agtgaggcca
     1501 aggcccggct gacccaaatc tggcctcaac gttccgtcgg tcggttcgac gacgcagtcg
     1561 aagatcattc gctcgatacg cacgtggtcg tgaaaatatt cggcataacg caatgccggc
     1621 gtcgcgcaac caatgtgaag atgcaatgcg ggcgcgcaat gtgacgaaag cgcgaggtga
     1681 tgcgcgtcgc aaagcgttgc tgcctgcaaa aatccagtga aacctccgca gcgggttgcg
     1741 tccgcctgca aaacatcgac agcgccagcc tcgatcatcc tacggaaata atcgatatcg
     1801 tatccgtatt cgcccgcggc gatctccatt ccggcggggg cgcgatcgcg aagtaaccgc
     1861 aagccttcca gatcgtctga tgaaactggc tcttcaaacc aggtgacgtt gaattccgca
     1921 aaggcttgcg cctgggccag tgcctgcttc cgcgagtagg cgccgtttgc atcgacgaaa
     1981 agttcgacat cttcaccaat tgccgcgcgc gcgatcgcga ctcgttgcct gtcgattgct
     2041 gcatcgcgtc caattttaat tttgacgcgc tggatcccct gctctaccca gccgcgaagt
     2101 tgtttctgca aatgatcgtt ggagtaattt gtgaatccgc cgcttccgta gatcggcatc
     2161 gcgtcgcgca cctgaccgaa cagcgtgacg agcggcaact cgagtaatcg agctttcaaa
     2221 tcccaaagcg ccgtgtcgac agctgagacg gccatcgaac aaatgcccgg tcgcccaaga
     2281 ttgcgaatgg aatgcaccat cgacatccat gcactggtca ccgacatcgc atcacaacca
     2341 acgacaacat ctttcagctt ttcctcaatt agctttgccg ttgcggtgtc ggcgtaggaa
     2401 tagccgagtc cgcgtttccc gccccccgtt acctcgacag tcacgatggt ggtgctgtcc
     2461 cattcgtaag ttccatccgc ttccggcgtt tcagtaggaa tcgtaaaggc ggaaacttgg
     2521 agctcgttta tctttccaaa ctggtagggg tcccgcagct gcgggaggcc gtccgataag
     2581 gaatgcgccg ccaacttctt cgtttgtcct tcgattgtgc gttccatttg tgatccgcat
     2641 ccggcgcgcc cggcagcttg tcctgagctt gtcgaatggg cgccctaccg tttctgtgcg
     2701 tcgcgcgcgc tcatttcttg tgaggcagga aaccttcgat cgcttccttg aaagtctgct
     2761 tgatcatgtg gccgagatgc ggctcgcctt taatcatcga ttcggtgaat ttcttcgcct
     2821 gctcaaacgt gatgtgcggt ggaagcgtgg gcacttccgg atcggttatc gcttcaagga
     2881 tgaccgggcg cgaggcgctt aaggcttgtt cccaggcggg cgcgatctgt tccagcttat
     2941 cgatgcgaat gccgctgagc ccgagcatct cggcgtaacg ggcaaattca aactgcggca
     3001 ccgtttgcga tacctgatac ttggggtcac cagccataac gcgttgttcc caggtgacct
     3061 gattgaggtc gcgatttgct agaaccagga tgacgaggcg cggatcgcgc cattgtttcc
     3121 aattctccgc gatcgtgacc acttcattga ttccgttcat cagcatcgcg ccgtcaccga
     3181 cggttgcgat cacgacgcgg tccgggaatg cgaacttcgc ggcgatcgca tatggcacgc
     3241 ctggtcccat cgtagccaga tttcccgaga gcgatgccat cataccgcgg cggactttta
     3301 gatcgcgcgc aaaccagttt gcagccgatc ccgaatcggc gacgataatg caattgtccg
     3361 gcaaccgcga cgacagttcc caaaaaacgc gttgcggatt aatcggtttg gcttcgttca
     3421 tcgcgcgtgc ttccaagatc tgccaccatt cggcgacgtt gttctcgatg tgctcgcgcc
     3481 attggcgttc ctgcttgcgc tcgagcagtg ggattaacgc gcgcaaggtt tctgcgctgt
     3541 cgcctaccag attaacttcc ataggatagc gcacgctgac gagctttggg tcgatgtcga
     3601 tttgcacgcc gcgcgcctgg ccttcctttg gtagaaattc cgaatacgga aaactcgagc
     3661 cgaccatcag cagcgtgtcg cattcttcca tcatgtccca gctcggcttg gagccgagca
     3721 ggccgatggg accggtgcaa aatggcagat catcaggaat aaccgccttg cctaacaagg
     3781 ccttggcaac gcctgctcca aggatatctg ccgtctcgat gatttcatcg gtcgcctgca
     3841 gggcgcccgc gccgaccagc atggcaactt tctgaccggc attcagaact tcggccgcac
     3901 ggcgaagatc gacatctctc ggcaccaccc gtggcgaagc ataatcggcg ccggagtgaa
     3961 tcgtgccatg tttacgcggc ggagtttcaa ctgcgggcat ttcctggacg tcattaggaa
     4021 taatgatgca agtgaccgtc cgctccgcct tcgcgatccg aattgcgcgg tcgatcaaat
     4081 gccgtatcgc tgcgggtgta gacgccatgt gcacgtactc gtgcgcaaca tccttgaaca
     4141 acgtaacgag atcgacttcc tgttgataat cgccaccgat cgctgcgcgc gcctgttggc
     4201 cgacaatcgc caccaccggc gcgtgatcca ttttcgcgtc gtaaaggccg tttagaagat
     4261 gaatcgcgcc cggccccgaa gtcgcgaggc aaattccgac ctcgccagta aactttgcgt
     4321 gcgcgcacgc catgaacgca gccatctctt catgacgcac ttgcacgtat tcaatggcgc
     4381 catccgcccg atcgatcgct ccaatgatcc cattaatgcc gtcgccggga tacccgtaaa
     4441 tgcgcttcag tccccactcg gtgattcgct tgatcaaaaa atcgctcgca gtgtcagcca
     4501 tgggatgaat tcaaagttga tggttgagtc ctgatagtca gtcgcgatgt caccaatcct
     4561 gaatcgcagc ggcaaggaaa tgcggacgct gataatatcc gcgttattcg cccgtcccta
     4621 tccgatccag acggaatcgt gtggcggcct gtttatcgaa cgtatgagca atccccatgc
     4681 gagatacaac cggcctgcac ctgagaatcg tctataaatt tgatgggaga cccacgaaac
     4741 atcgcgcacg ccaagaaagt cgtccaagag aagcatcata agaaaaagga ctctccgaag
     4801 gctcgtgccg ctaagcccca gccgaacagg gagcaacaaa ccgacgacaa acaatctgca
     4861 ggtggcgaga gttcagctcc caaggctgag gctgcgaagc cggatcaacc accagactca
     4921 acccagttgg agaaatcgct cgccgttcta tccactctaa aggatctgga gtttcattcg
     4981 cgggcgaata tcgaaaagtt agctgaactc agtctgacga ttgaggaaga gctcaagcaa
     5041 aaggcgtttg ctgagactat tggcgctgtt tattcggcgc aggacgcctt tcaatccaag
     5101 atcctgaaat tgattgaaga ctacgaagcc gagtgcacgc gcttgaaacc ggctgggtga
     5161 aatttcggtg tcaaccgtgg acgctttggc gcttgagcct ccgaattttt taacggaaac
     5221 cagccagcgt ataaatcagc cccagcaaac cgctgcccaa aaccacggga acaatattcc
     5281 acttccatcg cagcattccg aagaatgcga tcccacagat catcacagca aaccaatcaa
     5341 cgtgtccggt cgcgggaaaa atgacatgca agccaaacca aatagccaga ttcaagatca
     5401 cgcccacgac cgcagcagtg acagtcgaga gggcggatga gagggacctg atcccgcgta
     5461 atctctcaac atagggtgcg cccagtagaa tccagaggaa gcacggcaca aatgttgccc
     5521 aggtggtgat aagcgcaccg agtgtggcgg cgagaaagcg cgagagtcca ccgggatgat
     5581 tccaaccgcc gaggaaaccg acaaattgca gcaccatgat gagcggcccg ggcgttgtct
     5641 ctgccaggcc aagcccgtcg agcatctggc ccgcgccgag ccagtgataa ttttccaccg
     5701 cctgttgcga gacgtaggga agcaccgcgt atgcgccgcc gaaggtgacg agcgcggctt
     5761 tgctgaagaa gatgccttcg cgaaaaattg cgtgatccgt gccaagaaag attccagcga
     5821 gcgctatggg caaccaccaa agcaagagcg atattgcgca aattcgcagg catcgcccga
     5881 gtgtaggccg gccttggatc ggcagcgcac cctcacgatc gattgtcgat cctgcatttg
     5941 tcttaacgat ggccgggatt aacacgggtg agatttttcc cgcgataaat ccaaggacac
     6001 ccgcgctgat gatgatcatc ggaaacggca cgcggaaaaa atagattgca acgaatgccg
     6061 cggcagcgac gagccacatg atcacattac ggagcgcgcg ttgaccaatc cgaatgactg
     6121 cagcggcgac gatcgcgaga acggctggtt ttaatccgta aaggatcgca gcgatccagg
     6181 gaatgctccc atacgcaatg taaatgtaac tcagcgccca gaggataaag atcgagggga
     6241 tgacaaaaag cacgccagcg ataatgccgc cgcgcgtttt gtgcagcagc cagccgatat
     6301 agatcgccag ttgttgcgcc tctggtcctg gcaggagcat gcaaaaattc agggcatgca
     6361 ggaaacgcgc ttcgtcgatc cattttttct tctcgaccaa ctcggcatgc atcatcgcga
     6421 tctgaccgct cggtccgcca aaagaaatga agccaagctt taaccaaaag cggaatgcct
     6481 cagcaaacga cggcggaaga tgtcgatctt gtctggcaac tgtctgctct aacggggtca
     6541 gtttcatcgc cgctgcaaaa aggcgtaaag cgcggcaaag cactcaaaac cgcgacgcaa
     6601 gatctcatca tcaggcactc cctctttggc ccatcccttg aaaacacgat cgattcccac
     6661 gccctccact cgctgaaatc tgccatcgtc caagtcggcg tcatggatca tttcaccaat
     6721 tttcgcgact gatctatccg aaatagcaaa acgcttggtg agcgtttcaa atgtgcagca
     6781 attgccgtga tgtgagaatt cggcgtccag catgtcgaac ggaatcgctt ccggaacggc
     6841 ttgagccgtt ggcgcaaaga caaatcgtgc tttcggatcg atgaacttgg agatcaacca
     6901 cgctgatcct acccggtcga tctccggccg cggacgagtc agccacgtct tcccgcgata
     6961 ttgccgcgca tcgagcacct gaagttttcc cgagcgcctc ggtccttcag cgcggcgaag
     7021 cagcatcgcc acttcgtgtc cgcgcgcgct gtcgaagaaa tcgatctcac gaagttcgcg
     7081 aaactgtcga gtcagacgcg cgatttccgc catcacaagt tctgcatccg attttttttg
     7141 gcgcgcgatg aagctctgca ccgctttttt tagatcgcca tattctttgt cgcgcgcggc
     7201 attgaagaga tcgaccagtt tgtcgttggg taaaccttcg atctcgcgcg ctcgcaccag
     7261 tgtggcgtca ccacctgcgt cgcggatttc cttggttaac cactggaaac attcgtagcg
     7321 cgccggctcg tccggcaaga ggtaggtgga agtggttatc tgaattgccc cggatttttt
     7381 gagttttcgc cacatcgcca cgcgctcagc cttctgggtg gctggcaatc gaaaaagaag
     7441 caaaagccaa gtcagttttt tctttacagc agatcgcatg gttgatatat acctatacta
     7501 ctgagttaca catctaacaa tatgaaatcg ggtccgcgca ttttgctcat cagtaactca
     7561 accgtttatg gccggggcta tctcgatcat gttgaagcgc agatcaaaac tttcctcaac
     7621 gatgtcagaa acgtcctgtt ttttccattc gctttgtatg gccgcgacga ttacgcagcc
     7681 aaggcaaaag cgcgattcgc ttcgatgggt tactcggtgg aatcggcgca cgggatgcgc
     7741 gacccgcaca aagcattgga gcaagccgat gcgattttta tcggcggagg caacaccttt
     7801 cgactgctca aggctcttga ggatcttgat ctcatcgaac cgattcgccg gaaagtaagt
     7861 agcggagcac cctacatcgg cagcagcgcc ggctcgaacg ttgccgggcc aacgataaaa
     7921 acaacgaagg acatgccgat tgtccagccg cgatcattcg actcattagg cctggttccg
     7981 ttccaaatca gtccacatta tcaagatccg gatccagcct ccacgcacat gggcgaaacg
     8041 caggaggagc ggattctcca atttctcgag gaaaacgaaa cgccggtcgt cggcattcgc
     8101 gaaagcgcct ggctcgtgtg cgagagaggc gccgtgacat tgaagggcga aacgggcgca
     8161 cgaatcttca ggcgcggcga agctccagcg gaagcgaaac caggccggaa cattgtcgat
     8221 cttgtcggag gaccggatgg acactgagtc cacacttttg ctaagtcgcg gcgacgttga
     8281 ccagctgctc aacctcagcg actgcatcga tgcggtggaa aaagtctttc gccagcaaca
     8341 cgaccttgcg ttgcctggac ttctcggcat aaaaacacaa accggcggcc tgcacgtgaa
     8401 aacggcaatg ctgtcggccg agcgtcacta catcgtcgcc aaactgaaca ccaactttcc
     8461 gggaaaccgc gagcacttcg gactgccgac gattcagggt ctgcttctac tgtatgacgc
     8521 cgacagtggt cgagtgctcg ccgttatcga ttccatcgac atcacgatta agcgcaccgc
     8581 agccgcgacc gctgtggcgg caaaatatct ggcgcgaaag gattcatccg tcgcgacaat
     8641 ttgcgggtgc ggacaacaga gccgggcgca acttcgggcg cttctgttga ttctgccgct
     8701 aacgacaatc tgcgcgttcg atatcgacga gcgcgcggca attgcgttcg cagccgaact
     8761 ctcgcgcgaa ctcaagatcg acatcgaatt gatgcgcaac cttccggagg cgattcgcaa
     8821 cagcgatgtg tgcgtcactt gcacaacggc gccaaaattt ttcgtccgcc aggaagatgt
     8881 cgcgtccggc acgttcatcg cggcggttgg cgcagatgac gtccacaagc aagaaatcga
     8941 tccagctttg atcgcctcgg ccaaggtggt cgccgacagt ctgcaacaat gctgcgcgat
     9001 cggcgacacg catcacgcga tcgagaaagc gttgatgcga cgcgaaaacg tttacgccga
     9061 gttatcggaa attgtctccg gcgcaaaacc gggccgcata aacgaggacg aaatcatcat
     9121 cttcgatagc accggagtcg cgattgaaga cgctgcggcg gcgacgcttg tttacgagaa
     9181 agcgcgcagc acggcaatcc ggaaccagtt cgacttcgcg gcgttcgcag cgaatgccag
     9241 ataaaagcca atcagaaaag aaatgaggac gaaaccgctc acacgctcat ctgtgctgga
     9301 cgtcctacga cagctaatag cagtgccatc ggtcaatccc acgctcgcgc cgcacgaagg
     9361 tcagggtgaa ggaaacattg ccacgtttgt aaacaattgg ttcgcttcca aaggcgtcag
     9421 gagttggtcg gatgaagtcg cgccgaaaag gttcaatgcc gttgcacagc tcggggaagg
     9481 tcaaggtgct gcgctcgttt tttgtgccca cctggacacg gttagcacag acgggatgtc
     9541 gatcccgccg ttcgaggcgc gctgcgaagg gaatcgcgtc tatgggcgcg gcagctacga
     9601 catgaaaggc ggagccgcgg ccatcatgag cgcgatggca gcgctggctc tggatccgcc
     9661 acgcgggaaa gtatttgccg ccctcgttgc ggatgaggag tatgcgagca taggcgcgca
     9721 acacttcgtt gctcattaca aagcggacgc gtgcgtgctc acggagccga gtgaaggcag
     9781 tctaattctt tgtcacaaag ggttcgtgtg ggccgagatc gaaactcgcg gtcgcgctgc
     9841 tcatggcagc cgatgggatc tcggcgtgag cgcaattgga aagatgggca aaattgttac
     9901 cgctctggag gatttcgaca ggcagcaact ccgccggcgg gttcatccgc aagtagggcc
     9961 ggcatcccag cattcttcgc tcattgcggg cggaagcggc ctatcgacct acgcggaatc
    10021 ttgcacgctc aaagtcgaac ggaggactct gccaggcgag acgccagagc aagtgattcg
    10081 cgaattgcac gaggttgttt cgtccaccgg cgagaaagcg aaaatccgtc tgctgctcga
    10141 ccggccgccg ctgacttgct cgcgagacgc aaacatcgcg tcatgcctgc gcactgctgt
    10201 cacttcgacg ctcggcaaac cgccgccgga agcgggcgtg agttattgga tggatgcggc
    10261 gctatttgcg gcagcaggga tggacacaat cgactatgga ccgaccggcg gaggagcgca
    10321 tgaagcggtc gaatgggtag acctcgactc cgtggtgaat tgtgccgcga ttcttactga
    10381 aacagcaggt ctcttttgca atgaataaat gcccgcatcc agcgactcga tcatggattg
    10441 ccggcgcgca gttttctgcg cgagtcacgg aggcgcagag ttttttaagc gcctactttg
    10501 ctcccactcc tttgatcgct gctcgcagaa ttagcgcagc caccggatgt gatgtttacc
    10561 tgaagctgga atcatcgttg ccaaccggct cgtttaaacc gcgcggtgca ttgtttgcga
    10621 tctactcaca gctcaaagag aagccgattc gggaggtcgt caccgcgagc acaggtaatc
    10681 acggcgcagc tgtggcctat gctgcccggc aattcgggct ttcggcgaca atatttcttc
    10741 cgttcaattc gaaccgccag aaacgcagca agatcgttaa gttcggcgcc gccatccgag
    10801 aagctggcaa ggacttggcc gaggcagctg tggctgcggc ggcatatgca gcgaccgagg
    10861 acgcttatct actagatgac gcgagcgatc cggccgttcc aatcgcggcg gctacgattg
    10921 catgcgagat attcgatcaa cttccgaccg ttcagaaaat ttacgtgccg gttggcgaca
    10981 gtgcactgat ccggggaatt gcgtcggttg aaaagcggcg gccgctgccg ccccaactta
    11041 tcggggttca ggcagagcgt gcgccgtcat actatgattc gtggaaagcg gccgcggtgc
    11101 gcccatcgga cttgtgtgac accattgccg acggactcgc tacgcggcgt cctctggcga
    11161 gcaacgtcgc tgcgatgtgc aaactagtcg atgactttgt cctggtctca gaagaagcgc
    11221 taatcaattc ctttggaacg aggagaaaat ccttagcgag ccagcaggcg cagcatccac
    11281 agcagcgctg ctgaactcga aggcacaagc ctgcggcgat gtggtctgca tcgtcagtgg
    11341 ttgcaacgtc gccgacacgc taaagcagca agttctaacg gattctcgtt aacgcggccg
    11401 attatgaaat ggatcacacg cgaaaacatc aaagtggatc gcgtcgcgtg tccctggctg
    11461 attcgtcact ttgttgatgc cgacgccgaa tttgtttttc tcccggcaga cactgactgg
    11521 tcgaagatcg acaacggagc tgtgttcaat gtcccgaagt gcgagcttgg tcatcatggc
    11581 gaagacgttt cattcaattc gatcctggaa aaataccgcc tcggcgaccc ggccatggct
    11641 ttactgggcg aaatcgtgcg cgcagctgac tcgcatccga ccaatccgca tccagccggt
    11701 gaaggtttgc gctggatcgc gcacggcttc agcacagccg attttaccga tcacgaaatt
    11761 ctcgagcgga aatttgtcgt ttacgacgcg ctttatcgag agtccaaacg gcagatcaca
    11821 aaatgcgcga aggcggcgga acaagcgcct cgcacgaaaa ccgcgttgcc ttctttattt
    11881 gaaattcaga gcaccgttcg ctgactaagg ctaacagagg aaccagccct atgtttggta
    11941 ttcatgattt cgggttattt ttggcggcgg gcattttgct caacctgaca cctgggccgg
    12001 acacggctta cattctggga cgcagcattg tccagggccg cgatgccggc atcgcatcag
    12061 cgctgggaat ttgtgtcggc agtaattttc atacttgtgc agcggcgctc ggactttcgg
    12121 cgattcttgc gacctcggcc gtcgctttta gtacgatcaa attgctcggt ggcgcttacc
    12181 tgatttttct tggaatcaaa atgcttctcc atctgcgcaa agaattaagt ttgccctcca
    12241 attttcgtct gcgcacgacc gccgcggctt tccggcaagg cgtttttacc aatgtgctga
    12301 accccaaagt ggggctgttc tttcttgctt ttcttccgca gttcatcgat cccgcatcca
    12361 acacgaaggt cttggcgttt ctcgcgctcg ggctcacgtt tgtgacgacg ggcacaatct
    12421 ggtgccttat cctggcctgg ttcgcctccg ctttcagcga acgattgcgc gcgaacgaga
    12481 ccatcgggca gtggctcgat cgcgcagccg gtgcgctttt tattttcctg ggtttgcggc
    12541 tcgcgacagc aaagtgacca tctttgtcgg gcgatgccct gcgttcgtcg taattacttt
    12601 cctgcgccca aacgactctc cattttcgaa caagagttga gattaattgg tcaaaaataa
    12661 gtaactatag aaaataaata actatgaata caccaaaact attcacaatc gcagtggtta
    12721 gcgccgggct cgccttcggc gtggtcgcgc aggcaggcga aaagaaacac gaagagcaag
    12781 agatcagtag ctctgatgtg cccgcggcag tgcaacaagc tgccgacagc cacgccaaag
    12841 gcggaaaaat tgtccgctgg gaaaaagagg gcgcggacta cgaagccgtc atcgaaaaac
    12901 agggcaagga atggggcttc aagttcgatg cgaacggcaa aatgctaggc aagcacgacg
    12961 agagcaaaga aaagggcgaa aagggagaga aatactaacc ggaaggttgc ggttccggcc
    13021 gcaaagtatt ggggcggaac cagcaacgct cacagaaaat agccgctcct cggattggcg
    13081 ctctgctgcg cgaacattcg tcttatgttc gcgacgctcg attcgatcga gggcctcagg
    13141 tatgagtgag ccaccggcgg tcgggccggt acggtaccgc caaagaggca gcgaggatca
    13201 cctcttagct ggcagtgcaa aatcccgcgg ccggccaata gacgatgtct aatgatgcgc
    13261 cgatatcttg agtgacaaac aaaaaaaaga gccacacatg aaaacaaaaa tgacaacacg
    13321 aacacgctgg ccgctgattg cctgcgtcgc cctcgccaca atcgcgagct cgcccgcggc
    13381 ggatctggac acggcgaaga tcgatcagat cacgggcctc aaaggcaaac tgaacgagaa
    13441 ggaaggcgtt tataaagtca cctttccgcg caatgacgtg aagatcgttg tcgatggatg
    13501 gacgatgccg ccgttcatgg gactgggcac ctgggctgcg tttacgaaag gcgcgcacag
    13561 cgaagcgatg gtgatgggtg acaccgttct ctttgaagac gaagtgaatg ccacaatgac
    13621 cgccgcgctg gacaacgggc t
//
LOCUS       DASQUQ010000108         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1102347, whole genome shotgun sequence.
ACCESSION   DASQUQ010000108 DASQUQ010000000
VERSION     DASQUQ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102347"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VE486_03070"
     CDS             complement(<1..849)
                     /locus_tag="VE486_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZA38098.1"
                     /translation="MKLKLMQRDPSTALRSARDDGGGRAQTTAEILKKIRTLEIKTRG
                     LVETAFAGDYHSVFKGRGMNFEDVREYQPGDEIRAIDWNVTARMGTAFVKKFTEEREL
                     TVMLVVDVSASGNFGSVSQSKRELAAEVACLLAFSAIRNNDKVGLILFTDRVELFIPP
                     KKGRSHTLRLIREILFFEPQGRGTEPALALDYLNKIVTRRAVVFFISDFQAPDFSRAL
                     AISGRRHDFVAIHIHDEREKALPNIGIITLEDAETGDQIEINTSDRATRKRFSDLVNE
                     QGTELSR"
     gene            complement(1210..2196)
                     /locus_tag="VE486_03075"
     CDS             complement(1210..2196)
                     /locus_tag="VE486_03075"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZA38099.1"
                     /translation="MKSTQEITQEVRELGQWIPPLREQVGHVVVGQKYLVDRLLLGLL
                     ANGHILLEGVPGLAKTLTVKTMAACIQTGFQRLQFTPDLLPADLIGTLIYNPRTGEFT
                     TKLGPIFSNLILADEINRAPAKVQSALLEAMQERQVTIGEQTYPLSDPFLVLATQNPI
                     EQEGTYQLPEAQLDRFMFKLNIGYPEKAEERTILDLMATSAPDLRVNPVIEPKQIIAA
                     RNVVNSIYIDDRVKDYIVDVVWATRQPATYNLRLEGLIRYGASPRATIYLALGARAHA
                     FLNGRGYVTPQDVKSIGPDVLRHRIIVSYEAEAEAVTSETIIDRIFAGLPVP"
     gene            complement(2249..>2516)
                     /locus_tag="VE486_03080"
     CDS             complement(2249..>2516)
                     /locus_tag="VE486_03080"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38100.1"
                     /translation="VLNRLLRAAFAANPSPMISGRRLKLLYAAQASGKQERPLQPPKF
                     VLFVNEPRLLSETYRRYLEARIRKAQPYPGLPIILTLRPRSQKS"
BASE COUNT          540 a          682 c          692 g          602 t
ORIGIN      
        1 gcgcgaaagc tctgttccct gttcattgac cagatccgag aaacgcttcc gcgtcgcgcg
       61 atccgatgtg ttgatctcga tttggtcgcc cgtttcggca tcttccagag taatgatgcc
      121 gatattcggc aacgcctttt cacgttcgtc gtggatgtgg atcgccacga aatcatgtcg
      181 ccgcccgctt atcgccagcg cccgcgaaaa atccggtgct tgaaagtccg aaatgaaaaa
      241 gaccaccgcg cgtcgcgtga cgattttatt cagataatcg agcgccagtg ccggttctgt
      301 cccgcgtccc tgcggttcaa aaaacagaat ctcgcggatc agccgcaggg tgtgcgaccg
      361 gcctttcttt gggggaataa aaagttcaac gcgatctgtg aagagaatga ggccaacttt
      421 gtcgttattc cggatcgcgc tgaaggcgag caaacaggcg acttccgcgg ccagctcgcg
      481 cttggattgg gatacgcttc cgaaatttcc cgaggcgctc acatcgacaa caagcatgac
      541 agtaagctca cgttcctcgg taaatttttt tacaaacgcg gtgcccatcc gtgctgtcac
      601 gttccaatcg atcgcgcgta tttcatcgcc cggttgatac tcgcgcacat cttcaaaatt
      661 catcccacgc cctttaaaca cgctgtggta gtcaccggcg aaggcggtct cgaccagacc
      721 gcgtgttttg atttccagcg tccgaatttt cttgagaatc tcggcggtcg tctgcgcgcg
      781 cccacctccg tcatctcgag cggagcgaag cgcagtcgag ggatctcgtt gcattagctt
      841 cagtttcatt tgacggccca catttcggtg atcaagtttc ggcattcggg atttgatctc
      901 gcgattagcg cctccctttt gcttcgtcgc cagcgcttca attgtttctc acgcgcaatt
      961 gcacttctcg gatcaggaaa cgcttcgaag tggatcaatt tatccgtgtt gtattgccgt
     1021 gcgaagtggc tgcgtcgact cgcgccgtgc tcccaaagac gccgcaccaa atcatttgtg
     1081 attccgatgt acagaaccgt gtgtctttga ttggtgagaa tgtaaacgaa ataattttga
     1141 tgccgcattg ctaacttttc ggattcatca cgggatccct cgacttcgct cgggatgact
     1201 agaagatcat cagggcaccg gtagtcctgc aaaaatccgg tcaatgatgg tctcgctggt
     1261 gactgcttcc gcttccgcct cgtagctgac gatgatgcga tgccgcaaca cgtcagggcc
     1321 gatgctcttt acgtcctgcg gcgtaacgta accgcgaccg tttagaaacg cgtgagcgcg
     1381 ggcgcccagt gcgaggtaga tcgtcgcgcg cggcgaagcg ccgtaacgga tcagtccctc
     1441 gagccgcaag ttgtacgtcg cgggctgacg ggtcgcccaa acaacatcga caatgtaatc
     1501 tttcacgcgg tcatcgatat aaatactgtt aaccacgttg cgcgccgcga tgatttgctt
     1561 tggctcaatc acgggattca ctcgcaaatc gggcgccgaa gtcgccatta aatcgaggat
     1621 ggttcgctcc tcggctttct ccggatagcc gatgttcaat ttgaacatga aacgatcgag
     1681 ctgcgcttcg ggcaattgat aagttccttc ctgttcgatc gggttttggg tcgcgagaac
     1741 gagaaacgga tcggagaggg gataagtttg ttcgccgatg gtgacctgcc gctcctgcat
     1801 cgcttcgagc agggcgctct gcacttttgc cggcgcgcga ttgatctcgt cggccaggat
     1861 aagattggaa aaaattggac cgagcttggt cgtaaattca ccggtgcgcg gattataaat
     1921 gagcgtcccg atcaaatcgg cgggcaaaag atcgggggta aattgcaatc tttggaaacc
     1981 ggtttgaatg cacgccgcca tcgtttttac cgtgagcgtt ttggccagac ctggaacgcc
     2041 ctcgagcaga atgtgaccat tggcgagcaa accgagcaaa aggcgatcca ccaaatattt
     2101 ctggccaacg acaacgtgcc cgacctgttc gcgcaatggt ggaatccatt ggcccagctc
     2161 ccgaacctcc tgcgtaatct cctgcgtcga tttcatacgg tctggtgaga cacgtatgac
     2221 aaggagaatg ttcgatcaaa cgtggccgct acgacttttg gctgcgggga cgcagcgtga
     2281 ggatgatcgg caatccggga tacggttgcg ctttgcggat gcgcgcttcg agatagcgac
     2341 gataggtttc gcttaacaac cgaggctcgt tcacgaaaag gacgaacttg gggggttgca
     2401 gcggccgctc ctgttttccg ctcgcttgtg cagcgtataa caatttgagt cgccgtccgg
     2461 aaatcatcgg cgacgggttg gcggcgaagg ccgctcgcag caatcgattc agaacg
//
LOCUS       DASQUQ010000109         4553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_930195, whole genome shotgun sequence.
ACCESSION   DASQUQ010000109 DASQUQ010000000
VERSION     DASQUQ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930195"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..1350
                     /gene="trxB"
                     /locus_tag="VE486_03085"
     CDS             412..1350
                     /gene="trxB"
                     /locus_tag="VE486_03085"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZA38101.1"
                     /translation="MLDLFMEKVIIVGSGCAGLTAAIYAARANLSPLVLEGRQPGGQL
                     STTTLVENYPGFPDGIDGPQLIMNMHKQAERFGARFSYAEATDFEPRNSHVRIKADDE
                     WLETRALIIASGASARYLGLENEEKLIGHGLTSCATCDGAFYKNVPVCVVGGGDSAAE
                     ESLFLTRFASKVYLIHRRDKMRASKIMADRVFANKKIEPIWDTVVTKYIPDEKGEMRA
                     VRLRNVKTNEERELEVACVFVAIGHDPNTKAYAGKLETDPDGYLIAKNLAESKHPGVF
                     IAGDVADRVYKQAVTAAGSGCIAAMEAEKYLSELES"
     gene            1386..1778
                     /locus_tag="VE486_03090"
     CDS             1386..1778
                     /locus_tag="VE486_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZA38102.1"
                     /translation="MTERDLIERTKQFALRVIKLVGVLPRTIEGRAIASQLMRSGTSV
                     AANYRAACRARSKAEFIAKLGTVEEEADESAFWLELIIDAGLLSEPKTRSLLKEASEI
                     VAIMASSKKTAVRSGSQSQIANRKPQIR"
     gene            1775..2956
                     /locus_tag="VE486_03095"
     CDS             1775..2956
                     /locus_tag="VE486_03095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA38103.1"
                     /translation="MKICDLTQFYSPLSGGVKRYVHEKITYIDKHSPDDEHVLVVPGP
                     KTEVTTNARSRIYSIYSPLLSRTSRYRALLNLRAIEEILERERPDIIESSDPYQIGWK
                     AIAVGRSLKIPVVGFYHSHFPEAYLRGSTKFLGRRGTDRAMNLTRAYVRKLYNRFQAT
                     LVPSENLAGVLSEWGVCNVRTVRLGVNIDIFKPAGHDWDSTRKSLRMDRDQKLLLYVG
                     RLAKEKNTQTLFGAFELLQERRPNKFHLLVIGDGPQRTELRKLQSRSCGTDLSWIQYC
                     TDSADLARYYRAADLFVHPGVQETFGLVALESQACGTPVLGIRGSYMDQIICHDQDSW
                     ARANSAEALADAIVGASTKKLSALGKSAARAAENLYGWPRVFEELFCIYRETCANYRR
                     S"
     gene            2957..3652
                     /locus_tag="VE486_03100"
     CDS             2957..3652
                     /locus_tag="VE486_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38104.1"
                     /translation="MKSDDHKPFIIRSYRRSDREAIRKLCCDTGFLGDPIDPVYEDRE
                     LFADFLTTYYTDHEPESCFLLEVDGGIRGYLLGSRKPLQNQLYALYQNVWLFFRALTR
                     YLRYHERSRRFIRWTLVHGWHEVPARPRRVPHFHINLLPDARKVSTTRALMSAYLSYL
                     YRSGEKRVYGQIVTFESRRGEKMFERYGFKVLNRAEITKYKAFYPESVYLSTVIKNLE
                     TAEPLTAYSRTRT"
     gene            complement(3665..4024)
                     /locus_tag="VE486_03105"
     CDS             complement(3665..4024)
                     /locus_tag="VE486_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38105.1"
                     /translation="MAINVPVEIFVIKRLSVLKLVVISVVQPGQGTIVFVRMPKDPAF
                     LLQELAQSSIVSGVKGIVALLTVAGALTIAACSFFEPGEAVREESGRTLYRAAEENPT
                     DATEVTPVREKVPGESR"
     gene            4045..4518
                     /locus_tag="VE486_03110"
     CDS             4045..4518
                     /locus_tag="VE486_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HZA38106.1"
                     /translation="MKIGTPNSREIERKYLLKRLPEKLKRSRRYVIAQGYLATEPAGR
                     QVRLRKKGKTASLTFKVGRGRSREEREIKLSPKQFAALWPATAGRRLYKLRYEIPWRN
                     LMIEIDIYRRRHSGLVVAEVEFPDRATCGKFKPPSWFGREVTGEKRYSNVRLANE"
BASE COUNT         1049 a         1236 c         1304 g          964 t
ORIGIN      
        1 ggtcatgttt gttcgggtga aaggtcccgg attcgacgcg aatcctcccg gtcatgccga
       61 gatggatccc gagccagtta tcgctcgaga atctgaagag catccgtttg ccgcgcgccg
      121 ccgagttccg aaatttttcg ccgaccaatc ttcgacaaag ttcgcggctg cctattccgc
      181 gaaaaccctg tttcccgcat gtaaccgaac atcgacaatg tttttgccaa ggccggcgtt
      241 ctattgcttg cgaaaccatt cgacctcagc caactcgggc attgacggaa catagccgtc
      301 cacggctgtg cggccagcgg cgttgaactc cgctgagtca tcaagcggcg gtcaaactgt
      361 ccgccggccg cacaggcaac atgcctgtgt tccttggcgc gcttgcgcgg catgctagat
      421 ttattcatgg agaaagtgat cattgttgga agcggctgcg ccggcttgac ggcagccatt
      481 tacgccgctc gcgcgaattt gagtccgctg gtcctggaag gccggcaacc gggcggacag
      541 ctttctacca cgacgttagt ggagaactat ccgggatttc ccgacgggat tgatggtccg
      601 caattgatca tgaacatgca caaacaagcg gagagatttg gcgcccgctt ctcttacgct
      661 gaagcgaccg acttcgaacc gcgcaatagt cacgttcgca tcaaagccga tgacgaatgg
      721 ctggagacac gggcgctgat catcgcgagc ggggcttcgg cccggtattt aggactcgaa
      781 aacgaagaga aactgatcgg tcacggttta acttcatgcg caacatgcga cggcgcattt
      841 tataaaaatg ttcccgtatg cgtagtcggt ggcggggatt ccgcggccga agaatcgctt
      901 tttctcaccc gctttgcctc gaaagtttat ttgattcatc gacgcgacaa gatgcgtgcc
      961 tcgaagatca tggccgatcg cgtgttcgcg aacaaaaaga tcgagccgat ctgggacacg
     1021 gtcgtgacga aatatattcc cgatgaaaaa ggcgagatgc gggcggtgcg actgcgcaac
     1081 gtgaaaacga acgaggagcg cgaattggaa gtcgcgtgcg tgtttgtcgc cattggtcac
     1141 gatccaaaca cgaaagctta tgccggcaaa ctcgagaccg accccgatgg atatttgatc
     1201 gcgaaaaacc tggcggagag caaacatccc ggcgtattca tcgccggcga cgtggcagat
     1261 cgcgtttaca aacaagcggt cacggcggcg ggatcggggt gcatcgcggc catggaggcg
     1321 gagaaatatt taagcgagct ggagtcttga ttcgcaattt gcgagttgcg aattgcgatt
     1381 tgcaaatgac tgaacgggat ctaatcgagc ggacgaagca gtttgccttg cgagtgataa
     1441 agcttgtggg cgtgctcccg cgcacgattg aagggagggc gatcgcgagt cagttgatgc
     1501 ggagcggaac atcagtcgcg gcgaattacc gtgccgcgtg tcgcgcccga tcgaaggcag
     1561 agttcattgc gaagcttggc acagtggagg aagaagcgga cgaaagcgcc ttctggctgg
     1621 agttgatcat cgacgcgggc ctgcttagcg agcctaaaac tagatcactc ctcaaggagg
     1681 cgagcgagat tgttgcgatc atggcgtcgt cgaaaaagac cgctgtgcgc tccgggtcgc
     1741 aatcgcaaat tgcaaatcgc aaaccgcaaa tccggtgaaa atctgcgacc tcactcagtt
     1801 ctactcgccg ctcagcggcg gcgtgaagcg ctatgtgcac gagaagatca cctacatcga
     1861 caagcactcg cccgacgatg agcacgttct cgtcgttccg ggaccgaaaa cggaagtcac
     1921 tacaaatgcg aggtcgcgca tttattcgat ttactcacct ttgctctcgc gcacatcgcg
     1981 ttatcgggcg ctactaaacc tacgcgcaat cgaggagatc ctggaacgtg aacggcctga
     2041 catcattgaa tcgagcgacc cttaccaaat cgggtggaag gcaattgcgg tcggtcgctc
     2101 gctgaaaatt ccggtcgttg gtttttacca ctcgcatttc ccggaagcgt atctgcgcgg
     2161 tagcacaaaa ttcctgggca gacgcgggac ggatcgggcc atgaacttga cccgcgcata
     2221 cgtacgcaag ctttacaatc gctttcaggc cacgcttgtg ccctccgaaa atctggccgg
     2281 cgttctttct gagtggggtg tgtgcaatgt gcgcacggtg cggctcggag tgaacatcga
     2341 catcttcaag ccggcgggcc acgactggga ctcaacgcgg aagtccttac gcatggaccg
     2401 cgaccagaaa ttgcttcttt acgtgggccg tttggcgaaa gaaaagaaca cgcaaacact
     2461 ctttggagcc ttcgagcttt tgcaggagcg ccgcccaaat aaatttcatc tgttagtcat
     2521 cggcgacggg ccgcagcgca ctgaacttcg aaagttgcag tcccgcagct gcgggacaga
     2581 cctctcctgg attcagtatt gcaccgactc cgcggatctc gcgcgttatt atcgagcggc
     2641 ggatctattc gtccacccgg gcgttcagga aacgttcggg cttgtggcgc tggaaagtca
     2701 ggcctgtggc acgccggtgc taggaattcg cggcagctac atggaccaga ttatttgcca
     2761 cgaccaggac tcctgggcgc gtgcaaacag cgccgaggca ttggccgatg cgattgtagg
     2821 agcgagcacg aaaaagttat cggccctggg caagagtgcc gcgcgtgcgg cggaaaatct
     2881 gtatggctgg ccgcgcgtgt tcgaagaact gttttgcatt tatcgcgaga cttgcgcaaa
     2941 ctaccgccgt tcttaaatga agagcgacga tcataagccg ttcattattc gcagctaccg
     3001 ccgatcggat cgcgaagcga tccgaaagtt gtgctgcgat acaggatttc tcggcgaccc
     3061 gatcgatccg gtttacgagg accgggaatt gttcgcagat tttctaacaa cctattacac
     3121 cgatcacgaa ccggaatcat gctttcttct ggaagtggac ggcggaattc gcggttacct
     3181 cctcggctcg cgcaagccgt tacagaatca actctatgcg ctttatcaaa acgtctggct
     3241 cttttttcgc gcgctcactc gctacctccg ctaccatgag cgctcgcgtc gttttatccg
     3301 ctggacgctg gtgcacgggt ggcacgaagt gcccgcgcgt ccccgccggg tcccgcattt
     3361 tcacatcaac ctgctccccg atgcccgaaa ggtatccacc acccgcgccc tgatgagcgc
     3421 atacttgagt tatctgtatc gctcgggcga gaaacgtgtg tatgggcaga tcgtgacctt
     3481 cgagagccgg cggggggaga aaatgttcga acgctacgga ttcaaagtat tgaatcgggc
     3541 ggagatcacc aagtacaagg cattctatcc cgagtcggtc tatctcagca cggtgatcaa
     3601 gaatctggag acagccgagc cgctcacagc gtattcgcga acgcgcacct gaaagcgccg
     3661 gaaatcagcg ggattctccc ggcacttttt cccgcactgg cgtcacctcc gtggcgtcgg
     3721 ttggattttc ctccgcagcc cgatagagag ttcttcccga ctcctctcga actgcctcgc
     3781 ccggttcaaa gaagctgcaa gcggcaatgg tgagcgcgcc tgcgacggtg aggagagcga
     3841 caattccttt cactccgcta acaattgagg attgcgccaa ttcttgcaac agaaacgcgg
     3901 gatcttttgg catccggacg aaaacgatag tcccttgccc aggttgtacg acggaaatga
     3961 ccactaactt caagacgctg agccttttga tgacgaaaat ctcgaccggg acattgatcg
     4021 ccattagcgt cctgaccttg ccgaatgaag ataggaacgc caaacagtcg cgagatcgaa
     4081 cgcaaatatt tactcaagcg gctgcctgaa aaactcaagc ggtcgcgccg ttatgtcatc
     4141 gcacaaggtt atctggcgac agagcccgca ggcaggcagg tgcgcctccg caaaaaaggc
     4201 aaaaccgcgt cactgacttt caaagtgggc cgtggcagat cgcgggaaga gcgcgagatc
     4261 aaactcagtc ccaagcagtt cgcggctctt tggccagcga ccgccggcag gagattgtac
     4321 aagttgcgtt atgaaattcc gtggcgaaat ctaatgattg agattgatat ttatcggcgc
     4381 agacactccg gtttggtggt ggcggaagtg gaattccccg accgtgcaac gtgcggcaag
     4441 ttcaagccac cttcctggtt tggccgggag gtcacgggtg aaaaacgtta cagcaacgtg
     4501 agattggcca acgagtaagc agacaatatg ggcttccgtt tgaaattgcg cga
//
LOCUS       DASQUQ010000110        13477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1190556, whole genome shotgun sequence.
ACCESSION   DASQUQ010000110 DASQUQ010000000
VERSION     DASQUQ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 13477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13477
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190556"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VE486_03115"
     CDS             <1..222
                     /locus_tag="VE486_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38107.1"
                     /translation="WMIPKNQTDLSDQGTFFSLVIIYLMNLLLLSVMLVLASPEITFA
                     DFGTDLLTNLSHFAQWVVELFDRVSRGAQ"
     gene            complement(363..3221)
                     /gene="treY"
                     /locus_tag="VE486_03120"
     CDS             complement(363..3221)
                     /gene="treY"
                     /locus_tag="VE486_03120"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HZA38108.1"
                     /translation="MTEKSHPRIPSSTYRLQFNRWFTFAQARAIVPYLHALGVTDAYA
                     SPYFQAGAESLHGYDITDHNKLNPVIGSREEYDAWVAELHAHSMGQVLDFVSNHVGIA
                     EPLNQWWMDVLENGPSSRFAPYFDIDWHPLKSDLRDKVLLPILSDQYGRVLERGELQV
                     RFEEGTFYLLCGERRLPIAPGTYRYVLNIALQNLAEYRDEDFYAELQSILTALEYLPK
                     RTETDPKRVAERVREKEIIKRRLERRCAEAPQVQHAIEKALAQINGEPGDPRSFDTLD
                     ELLNAQSYRLAYWRVAAEEINYRRFFDVNDLAAIRVELAKVFDAVHRLVLDLVRTGAV
                     AGLRIDHPDGLYLPREYFEKLQQRCAKALAIPLPKDGRAIYMVAEKILTGPETLRRDW
                     LVHGTTGYDFANQVAQLLVDSSAETPITKTFHRFIGHSLPFGHLLYAKKLQVMKLSLA
                     NDVDVLGNMVDRLSEQNRWYRDFTLEALARAVRETIACFPVYRTYLAPGRPVSEEDRQ
                     IVERAVAAAKRRNPAIEESIFNFLRDVLVFRFPENLDAEARAAHTHFVLKFQQTTGPI
                     MAKGLEDTVFYIYNRLVGLNEVGGEPQQFGSGVEAFHERNLDRQNNWPATMLATSTHD
                     TKRSEDVRARIVAISEIPELWHRSLQRWRVANRRWKRMINDAEAPDANEEYLLYQTLL
                     GTWPIQDSGEPEGTANAEYIERIQAYMAKALHEAKLNTSWIQPNEEWDAAMRDFVAKI
                     LDSSPRNKFLPAFLPMTQEIARLGAINSLTQTALKLTSPGVPDIYQGNEIWDYSLVDP
                     DNRRPVDYQHRREMLAALPAAAPDELMRNWADGRIKMFLTQRLLQFRRQHADLFQRGE
                     YLPLKTNGTFAKCCISFARQLADKWIVVIAPRLSSHIGFPPVGERWRDTAIELPEKLS
                     LENAHDLFTCRAIRHEGQKVSMSDAMSILPFAVITNLP"
     gene            complement(3419..5167)
                     /gene="argS"
                     /locus_tag="VE486_03125"
     CDS             complement(3419..5167)
                     /gene="argS"
                     /locus_tag="VE486_03125"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZA38109.1"
                     /translation="MTRRSDYSLWLLEIMETFQSLLAKKLSDALAKAGLPNAGELTPA
                     TDPRFGDYQTNAALILGKQRGENPRTLAEKILAQLDVGDLCEPPTVAGAGFINLTLRA
                     DVIARKAGEVLRNERLGVPKAISTRRIVIDFGSPNVAKPMHAGHIRSIVLGDALARIA
                     QFLGYEVIRDNHVGDWGTQFGMVIYGWKNLLDRQALQRDPLAEIVRIYKETNESAAKD
                     PQVRETCRQELVKLQAGDREDIDIWNECVALSMQDFDHVYKLLDIHYDIQCGESFYND
                     RLPGVVERLLKSGIAEISEGAVVVFFRDIPELADKPCIIRKRDGGFNYATTDLATVDY
                     RVNDLKADAIWYVVGAPQILHFKQIFKIARREGYKADFRHIAFGSILGEDRKLMKTRS
                     GENVPLRDLLEEACRRARRIIEEKNPDLSEADKMEIAQKIGIGAVKYADLSQYRMTDY
                     VFSWEKMLSLQGNTAPYLQNAYVRVRSIFRKAGESAPKVDELVLGDAAETNVAKRLCQ
                     FAEIVPQVLNDFRPNILANYLFELANSFHTFYEACPVLKSEEPVRSSRLALCELTGRV
                     LQRGLDLLGIKVPEKM"
     gene            complement(5264..5725)
                     /locus_tag="VE486_03130"
     CDS             complement(5264..5725)
                     /locus_tag="VE486_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZA38110.1"
                     /translation="MSLASLLSAEQIIPEMKATDRWPAIVELIDLLVSLGKIKSADRD
                     SILESLKQREETMSTGIGFGIAIPHCSSDRLDEVVAAFGRSSGGIEFDALDNAPVKFV
                     VLFIVPKNQFQTHLRTLASIAKFLNDRSVRESLAEAKSADEILSVFRDRSK"
     gene            complement(5826..6539)
                     /locus_tag="VE486_03135"
     CDS             complement(5826..6539)
                     /locus_tag="VE486_03135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZA38111.1"
                     /translation="MTEVADVPAVSVVVPLFNEEENVPILQSELRAALSGLDHEIVFV
                     DDGSVDRTVERIETGPNIRVIRFEKNTGQSAAIYAGLQAARGVTAVLIDGDLQNDPAD
                     IPRLLAEIARGADLVCGHRQERKDTLAKRLTSWIANAVRSRFTKDGVRDTGCTLKAMR
                     RECVSALIPFKGMHRFIPALIKGAGYRLVEIPVNHRPRRFGQSKYGLGNRAFRATIDM
                     FGVRWLLARRLNYKVRERM"
     gene            complement(6536..8113)
                     /locus_tag="VE486_03140"
     CDS             complement(6536..8113)
                     /locus_tag="VE486_03140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZA38112.1"
                     /translation="MSITRAVWFFVLALTAIRLSMLATMDLEFDEAHYWMWSERLAPA
                     YFSKGPGVAFAMRASTAVFGANEFGVRFFSPLLAAGTSLLLFYFARRLFSASAALWAV
                     IALNVTPIFNIGAFLMTIDALSIFFWLAAMFTFWLAVEKSPRFSWHWPLTGLLIGLGF
                     LSKYTNAFELVSILLVLALAPRLRQEFARPGLYSLLGMFALCTVPPIVWNAQHAWITL
                     VHLRSRGGVEEGFGFHPGELLAFLIQHFLAYSPFLFLALAWGVIGSWRRVNQQFKVLF
                     LMWFGLPVFAFYLLLSINKTAAPNWDGLAFFGFGLLAIYFWWKRLEASALLRVCANVA
                     LLVGLAMSVIALNTDLLRTAGYELPRNDPSDRMRGWKSATGAVEKIRNDLEAKLGEKL
                     FLIADARDRASEMSFYLRDKRPEGPGHPPVYITESQDLVNQFSFWPRYDEFVEVKPGT
                     PRPRDEVYTEESGINPFVGRSALFIRQGEKEHPPHNIRAAFQSTEPVGTIEVRRHDKV
                     IRRWQVFLCRNYRTLPL"
     gene            complement(8110..8814)
                     /locus_tag="VE486_03145"
     CDS             complement(8110..8814)
                     /locus_tag="VE486_03145"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZA38113.1"
                     /translation="MDQTLFHLVNERWTSPALDLFMAALSNSEIWKPLFIGIGLSALL
                     FGGFKGRAFVVCLVLSLLIAVLFTDILKPAVDRHRPKHVESVRMVEMQRTHVKFMTLF
                     KKTTIRFSDQSDRNRSGPSFPSGHMTNNTVIAVYCTLFFRRRGWLYWIVAAAVGYSRI
                     YLGAHWPSDILASLFLAAAEALLTLGLFEWIWRTVAPKWAPNVYARHPSLIGDSNGRE
                     RPEPAPRGGGAPHHTL"
     gene            8925..9653
                     /locus_tag="VE486_03150"
     CDS             8925..9653
                     /locus_tag="VE486_03150"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HZA38114.1"
                     /translation="MTKVRSGAFFLSLGFRHSFVIRHSSFVICSGLALSIHAQPVPSA
                     QDILASVRMMEAQQQLDLQGQLRQNDVIIPFRLTQNGPLIRYSFTNPDQVLQLRLGEK
                     SSRLDLVTGAGTEKFAAPKLNQKIRGTSVSYEDLAFKFLYWPAARVLGEENVRTRNCW
                     KLQLRAPSRESQYSNVLLWVDKASGALMKMEGYDWNAQLAKRFEVVSGQKIGSRWFLK
                     QMRIEELQPGTTHALSRAYLEIKK"
     gene            complement(9728..10492)
                     /gene="tpiA"
                     /locus_tag="VE486_03155"
     CDS             complement(9728..10492)
                     /gene="tpiA"
                     /locus_tag="VE486_03155"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002144919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZA38115.1"
                     /translation="MRKKIVAANWKMNMTQAESARFVESLLLDLGDITDIEVVIVPPF
                     TAIARVMEALDKAQNIKVGAQNMHWERNGAFTGEISAALLRDLFVHYVVLGHSERRIL
                     FGETDEIVNLKVRAAHEATLRPIVCIGETLDQRDKGNVEKILAIQLRGSLKDLSEKEL
                     QETVIAYEPVWAIGTGRNATPEQAQEAHMYIRETLRDMANDATAERVRIQYGGSVKPE
                     NAHELMSQPDIDGALVGGASLDPRSFAQIVKAARPD"
     gene            complement(10558..11766)
                     /locus_tag="VE486_03160"
     CDS             complement(10558..11766)
                     /locus_tag="VE486_03160"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZA38116.1"
                     /translation="MKLTLRDLDVRGRRVLVRVDFNVPTDTRGGKVHITDDTRIRESL
                     PTINYLREHGAKTILMSHFGRPKGKRVQKYSLRPIGDYLHSLIHQPVIFSHDTIGDVP
                     EKIINHMENGDVALLENLRFQPEEEANDPKFAEALAKLGDLYVNDAFGAAHRAHASTV
                     GITKFVAKSAMGFLMEKELKHLRDGLDKPTKPFVVILGGAKVSDKIGVLKALMEKADT
                     ILIGGAMANTFLKAQGIPVGASRVESDKVDVAREILDLAKKRGVKFLLPIDVVEAEEI
                     HTGAAIQNTSRLSSQHGITDGWQAVDIGAATIALYQEEIAKGETILWNGPVGIFEIPD
                     FSEGTIAIAEALAQSGATTIIGGGDSVTAVKQAGLADKMTFISTGGGASLELLEGKEL
                     PGVAALSEKE"
     gene            complement(11854..12855)
                     /gene="gap"
                     /locus_tag="VE486_03165"
     CDS             complement(11854..12855)
                     /gene="gap"
                     /locus_tag="VE486_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZA38117.1"
                     /translation="MPNYAINGFGRIGRNVLRAMTQKQVEQVRAINDLTDTQTLAHLL
                     KWDSVHGKFDGEIGYDEENIIVRGHKIRVLKERDPANLPWKKLDIDVVLESTGLFTSR
                     DKAELHLSKAGAKRVLISAPAKDPDVTICMGVNDQLYDPAKHHIVSNASCTTNCLAPL
                     AKVLNDKFGIAHGFMSTIHSYTNDQRILDFPHKDLRRARAAAINIIPSTTGAAKAIGE
                     VIPELKGKLNGGAFRVPTPDGSVTDFTAVLEKDATVDTVNAAFKEAASDKSYKGVVEY
                     SDEPLVSQDIVENPHSCIFDSKLTLMLGNRFVKVVGWYDNEWGYSTRCVELMELLGR"
     gene            12915..>13477
                     /locus_tag="VE486_03170"
     CDS             12915..>13477
                     /locus_tag="VE486_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HZA38118.1"
                     /translation="MKLGFAVIKSGLILVCLALPIVFAEFASTSPIDAGDMKRILVLG
                     DSLSDGFGLKRSEAYPALLADKLRAAGLNFQVTNASQTGGTTDGGLERLPAHLKPKID
                     IFILELGINDAFRGMPVDQIQNNLQEIIDKVKARNPNVRVVIAGMQLPNYGADDYVSA
                     FGKLFAELAAKNGAALVPYLLDGVAGDP"
BASE COUNT         2946 a         3855 c         3658 g         3018 t
ORIGIN      
        1 tggatgatcc cgaaaaacca gaccgacctc agcgatcaag gcacattttt ttcgctcgtg
       61 atcatctatt taatgaacct gctgctgctc agcgtaatgc tggtgcttgc atcgccggaa
      121 attacgtttg cagatttcgg cacggatcta ctgacgaatc tcagccactt cgcgcagtgg
      181 gttgtcgagt tgttcgaccg cgtcagccgg ggcgcccagt agattggcgt ttcacagaat
      241 ggagccagcg ggcgctgagc gacatagacg ggcaggcctg aggccgagcg agcggggcga
      301 gcgagtcaaa cgccctccaa ttcgtgacgc aattttgtag ggcgtctgtg tgcagacgcc
      361 tgttaaggaa ggttcgtgat caccgcaaag ggcaaaatgg acattgcgtc gctcatcgaa
      421 actttttggc cctcgtgacg aatcgcgcgg caggtaaaga gatcatgcgc gttctcgagc
      481 gaaagttttt cgggcaactc gattgccgta tctctccacc gttcgccaac gggaggaaag
      541 ccaatgtgcg aagagagccg cggcgcgatc acaacaatcc atttgtccgc cagctgccgg
      601 gcaaagctga tacagcactt cgcaaacgtg ccattcgttt ttagtggcag atattctccg
      661 cgctggaaaa gatcggcatg ttggcggcga aattgcagca gtcgctgggt caaaaacatt
      721 ttgattcggc catcagccca attgcgcatc agctcgtcgg gagctgctgc cggcaatgcc
      781 gcgagcattt cacggcggtg ttggtaatcg accgggcgac gattgtcagg atcgaccaga
      841 ctgtaatccc aaatctcgtt gccttgataa atgtcgggga ctcccggcga ggtcagcttt
      901 agcgccgttt gggtgagtga attgatcgct ccgaggcgcg caatttcctg tgtcataggc
      961 aggaacgctg ggagaaattt atttcgcggc gacgagtcca ggattttcgc gacgaaatcc
     1021 cgcattgcgg cgtcccattc ttcgtttggc tggatccagc ttgtgttcag cttcgcctcg
     1081 tgcagcgcct tggccatgta agcctgaatc cgctcgatgt actcggcgtt tgccgtccct
     1141 tcgggttcgc ccgagtcctg gatcggccag gttccaagca aagtctgata gagcaggtat
     1201 tcctcgttcg catccggcgc ttccgcgtca ttaatcatcc gcttccagcg gcgattggcg
     1261 acgcgccacc gttgcaggga gcggtgccaa agctccggaa tttcggaaat ggcaacgatc
     1321 cgcgcgcgca cgtcttcgct gcgcttggtg tcatgcgttg aggtcgcgag cattgtcgcg
     1381 ggccaattgt tttgccgatc gagattgcgt tcgtgaaagg cttcaacgcc tgatccgaat
     1441 tgttgcggct cgccgcccac ttcattcagc ccgaccagcc gattataaat gtaaaacaca
     1501 gtgtcttcca ggcccttggc catgatcggt ccagtggttt gctgaaactt gagcacgaaa
     1561 tgcgtgtggg cggcgcgcgc ttcggcatca agattttccg gaaaccggaa gaccaggacg
     1621 tcgcggagaa aattgaaaat cgattcttcg atcgccgggt tccgccgttt cgccgcagcg
     1681 accgcgcgtt cgacgatttg tcgatcttct tcgctcaccg gccgcccggg agcgagataa
     1741 gtcctgtata ccggaaaaca ggcgatcgtc tcgcgcacag cgcgggccag cgcttcgaga
     1801 gtgaaatccc gataccatcg gttttgttcg gagagtcgat cgaccatgtt gcccagaaca
     1861 tcgacatcgt tggccagtga caatttcatg acttgcagct ttttcgcata caagagatgc
     1921 ccaaaaggca gggaatggcc gatgaaacga tgaaatgttt ttgtgatcgg tgtctcggct
     1981 gaagaatcga ccagcaactg cgcgacttga ttcgcaaaat catagcccgt cgtgccgtgc
     2041 accagccaat cccgtcgcaa cgtttccggg ccggtgagga ttttctccgc gaccatgtaa
     2101 atggcgcgcc catccttggg gagcggaatc gccagcgcct ttgcgcagcg ctgctgaagt
     2161 ttttcgaaat attcgcgcgg caggtaaaga ccgtccggat ggtcgatgcg gaggccagcc
     2221 accgcacccg tgcgcaccag atcgagaacc agccggtgaa cggcgtcgaa aacctttgcc
     2281 aattcaaccc ggatcgctgc cagatcatta acatcgaaaa agcggcgata attaatctct
     2341 tccgctgcga cccgccaata ggccagccga taagactgcg cgttgagtaa ctcatccagc
     2401 gtatcgaagc tgcgcgggtc gcctggctct ccgttgatct gggccaatgc cttttcgatt
     2461 gcgtgctgca cttgtggcgc ttccgcgcaa cggcgttcga gacgtcgctt gatgatttcc
     2521 ttctcgcgaa cgcgctccgc gacacgcttc ggatcggttt cagtgcgctt tggcaaatat
     2581 tcgagcgcgg tcagaatgct ttgcagctcc gcgtagaaat cttcgtccct gtattcggca
     2641 agattttgta acgcaatatt caggacatag cgatacgtgc ccggcgcgat cggcagtctg
     2701 cgctcgccgc agagcagata aaaggtgcct tcttcgaacc gcacttgcag ctcgccgcgc
     2761 tcgaggacgc gcccgtattg atcgctcagg atcggcaaca ggactttgtc gcgcagatcc
     2821 gatttcaacg gatgccagtc aatatcgaaa tagggcgcaa acctggagct tggcccgttt
     2881 tcgagcacgt ccatccacca ttgatttaac ggctccgcga tcccgacgtg gttcgagaca
     2941 aaatccagca cctgacccat cgaatgcgcg tggagttccg cgacccaggc gtcgtactcc
     3001 tcccgtgagc cgatcaccgg gttgagcttg ttatggtcgg taatgtcgta gccgtgcagg
     3061 ctttctgcgc ctgcctggaa atagggcgat gcataagcat ccgtcacgcc cagcgcgtgc
     3121 agatacggca cgatcgcgcg cgcctgggcg aacgtgaacc agcgattgaa ttgcaatcgg
     3181 taggtcgaac tgggaatacg tgggtggctc ttctctgtca tttcctttgt cattccgagc
     3241 gaagtcgagg aacgatttca aatgacgaaa tccgaatgac ccgccttcgc gtagcttcgg
     3301 cgtggcaggc gaatgacgaa ccaaatccga atgacaaaac ccgaaacggc gcccacatcg
     3361 cggcccagtc tttcgttatt tggacttcgt cattcattcg gcattcgtca tttcgccatc
     3421 acatcttctc gggaactttg attccaagaa gatccagacc ccgctgcaag acgcgcccgg
     3481 taagctcgca caatgccaga cgcgagctgc gcaccggctc ttccgacttc aaaaccgggc
     3541 acgcttcata aaatgtgtga aagctgttgg ccagctcgaa gagataattt gccagaatgt
     3601 ttggacgaaa atcgttcaat acttgcggca cgatttcggc gaactgacac aggcgtttcg
     3661 ccacattagt ttcggcggca tcgccgagga ccagctcatc gaccttgggc gccgattcgc
     3721 cagctttacg aaaaatcgag cggacacgga catacgcgtt ctgcaaatat ggcgccgtgt
     3781 ttccttgcag cgacagcatc ttctcccatg aaaaaacgta atccgtcatc cggtactgcg
     3841 agagatcggc atatttgacc gcgccgattc cgatcttctg cgcgatctcc atcttgtccg
     3901 cctcgctcaa atcgggattc ttctcctcga taatcctgcg tgcgcgcctg catgcttctt
     3961 cgagaagatc tcgcaacggg acgttctctc ccgagcgcgt tttcattagt ttgcggtcct
     4021 cacccagaat gctgccaaac gcgatgtggc gaaaatcggc tttgtaacct tcccgccgcg
     4081 cgatcttgaa aatctgtttg aaatgcaaaa tctgtggcgc gccgaccacg taccaaatcg
     4141 catcggcttt caaatcgttg acgcgataat caacggtcgc gagatctgtg gttgcgtaat
     4201 tgaatccgcc gtcgcgcttg cggatgatgc atggtttatc ggccagctcg ggaatatcgc
     4261 ggaagaaaac gactaccgcg ccttcgctga tttcagcgat gccggatttg agcaggcgct
     4321 cgaccacgcc gggcaggcga tcgttgtaaa aactttcgcc gcactggatg tcgtaatgga
     4381 tatcgagcag tttgtaaacg tggtcaaaat cctgcattga gagcgcgacg cattcattcc
     4441 aaatatcgat gtcttccctg tcgcccgcct ggagcttcac cagctcctgg cgacaggttt
     4501 cgcgaacctg cggatctttt gccgcgcttt catttgtctc cttgtaaatg cgaacgatct
     4561 cggcgagcgg atcgcgctga agcgcttgtc gatccagcag atttttccac ccgtaaatga
     4621 ccatgccaaa ctgtgtcccc caatcgccta cgtgattgtc gcgaatgact tcatatccga
     4681 ggaactgcgc gatccgcgcc agcgcgtcac cgagcacgat gctgcggatg tgaccggcgt
     4741 gcatcggttt ggctacgtta ggcgatccaa aatcaatgac gatccttctc gtcgagatcg
     4801 cttttgggac accgagccgt tcgttccgca agacttcgcc cgcttttctt gcgatgacat
     4861 cggcgcgcaa tgtgagattg atgaagcctg cgccagcaac ggtgggcggt tcgcaaagat
     4921 cgccaacatc aagctgggcg agaattttct cggcgagtgt tctcggattt tcgcccctct
     4981 gtttcccgag aatcagcgcg gcgttggtct ggtaatcgcc gaaacgcgga tcggtcgccg
     5041 gcgtcagttc gcccgcattc ggaaggcccg cctttgccag cgcatcggaa agcttctttg
     5101 cgagaagtga ttggaaggtt tccatgattt caaggagcca cagactgtag tcgcttcgcc
     5161 gtgtcacgcg agaaggtgga tcgagcagtc ccttgctcga tgctaatcag gcggcggagc
     5221 cgcatttatt taacatcgcc cgacggagcg gtcgatccac caactatttc gagcggtcac
     5281 gaaagaccga taaaatttcg tcggccgatt tggcttcggc caggctttca cgaacgctgc
     5341 gatcattcaa aaatttggca atcgatgcaa gcgtgcgcag gtgcgtctga aattgatttt
     5401 tcggcacaat gaaaagaacg acgaatttca cgggcgcgtt gtcgagggca tcgaactcaa
     5461 ttccgccgga cgatcgtcca aatgcggcca cgacttcatc aagccgatcg gaggagcagt
     5521 gggggatggc gatcccgaat ccgatgccag tgctcatggt ttcctctcgt tgtttgagcg
     5581 actccagaat gctgtcgcga tccgcggatt tgattttgcc cagactaacc agaagatcga
     5641 tcagctcgac aatggccggc caacggtcgg tcgccttcat ctcggggatg atttgctcgg
     5701 cagaaagtaa gctggccagc gacatgcaga ttccccttga gtacgggagg gcaagcttag
     5761 cgggagcgcc taagttgaca agcgcgattt ggtacgctgg ccatatgccg catccgtctc
     5821 atctatcaca tcctctcgcg gactttgtaa ttcaatcgcc gcgcaagcaa ccagcgcaca
     5881 ccgaacatgt cgatggtggc ccggaaggcg cgattgccca ggccgtattt gctttggccg
     5941 aaacgacgtg gccgatggtt caccggaatt tccacaaggc gatagcctgc gcccttgata
     6001 agcgccggga taaagcggtg catgccttta aacggaatca gcgcgctcac gcattcgcgc
     6061 cgcatcgctt tcagcgtgca ccccgtgtcg cgcacgccgt ctttcgtgaa acggctgcga
     6121 acggcgttgg cgatccaact ggtcagccgc ttcgccagtg tgtccttgcg ctcctgccga
     6181 tgaccacaca ccagatcggc accgcgcgcg atctcggcga gaagtcgcgg aatgtccgcg
     6241 ggatcattct gtaaatcccc gtcgataaga actgccgtca cgccccgcgc agcctggagg
     6301 ccggcataaa tggcggcgct ttgtccggta tttttttcga agcgaatcac tcgaatattc
     6361 ggccccgttt cgattcgctc gacggtccga tcgactgatc cgtcatcaac aaaaacgatt
     6421 tcgtgatcga gcccgctcag cgctgcgcgc agctcggatt gcaggatcgg aacgttctcc
     6481 tcctcgttaa agagaggcac caccacggaa accgccggca cgtctgccac ctctgtcata
     6541 gcgggagcgt gcgatagttt cggcaaagga acacctgcca cctgcgtatt accttgtcgt
     6601 ggcgccgcac ctcaatggtc cccaccggtt cggtcgattg gaaggcggct cgaatattat
     6661 gaggcggatg ttctttttcc ccctggcgaa tgaagagcgc gctgcgtccg acgaaagggt
     6721 tgatgccgct ctcctcggtg taaacttcgt ctcgcggacg cggcgtgccc ggttttacct
     6781 cgacaaattc atcgtagcgc ggccaaaagg aaaactggtt taccaggtcc tgcgattcgg
     6841 tgatgtaaac gggcggatgc cccgggccct ctggtcgctt gtcgcgcaaa taaaacgaca
     6901 tttccgatgc gcgatcgcgg gcgtcggcaa tgagaaacaa tttctctccc agtttcgctt
     6961 cgagatcgtt ccggattttt tcgacggcgc ccgttgcact tttccatccg cgcattcgat
     7021 cgctcgggtc gttgcgcggc aattcgtagc ctgcggtgcg cagcaggtct gtgttcaacg
     7081 cgatgacgct catggccaat cccaccagca atgccacgtt cgcgcacact cgcagcaggg
     7141 cgcttgcttc gaggcgtttc caccagaaat agatggccag caaaccgaac ccgaagaagg
     7201 cgagtccgtc ccaatttggc gcggcggttt tgttaatcga caggagaagg taaaaagcga
     7261 agacgggcaa tccaaaccac atcaggaaca acaccttgaa ttgctgattt acgcggcgcc
     7321 agctcccaat gacgccccac gccagcgcga gaaagagaaa tggcgaatag gccagaaaat
     7381 gctggatgag aaacgcaaga agttcgccgg gatggaaccc aaacccctcc tcgacgccgc
     7441 cgcgtgatcg cagatgcact agcgtaatcc aggcgtgctg cgcattccac acaatcggcg
     7501 ggacagtgca aagggcgaac atcccgagca atgaatacaa accgggccgc gcaaattcct
     7561 gtctgagcct gggcgccagg gcgagcacga gcagaatcga aacaagctca aatgcgttgg
     7621 tgtatttgga gagaaatcca agcccgatca gcagccccgt gagcggccaa tgccaagaga
     7681 aacgtggact cttttccacc gccagccaga acgtgaacat cgctgccagc cagaaaaaaa
     7741 tcgaaagtgc atcgatggtc atcaggaacg cgccgatatt aaagataggt gtgacattca
     7801 aggcgatgac tgcccacaaa gcggcgctgg cgctgaacaa tcgccgcgca aagtaaaata
     7861 agagcaggct cgtgcccgca gccagcagcg ggctgaaaaa gcgcacgcca aattcattcg
     7921 cgccgaacac agctgtgctg gcgcgcatgg caaaagcgac gccaggtcct ttactgaaat
     7981 acgcaggagc gagccgctcc gaccacatcc agtaatgggc ctcgtcgaat tcgaggtcca
     8041 tggtggccaa catggacagt cgaatcgcgg ttagcgcgag aacgaaaaac cagaccgcgc
     8101 gcgtgatgct cataaagtgt ggtgcggcgc gcccccgcca cggggggcag gctctggtcg
     8161 ctccctacca ttcgaatcac caatcaggct cgggtggcgt gcgtaaacgt tcggcgccca
     8221 tttcggcgcc accgttcgcc aaatccactc gaacaatccc agcgtgagca aggcttcagc
     8281 ggcggccaga aacagcgacg cgaggatgtc gctcggccaa tgcgcgccga gataaattcg
     8341 cgagtaaccg acggctgcgg cgacgatcca gtagagccag ccgcgccggc gaaaaaacag
     8401 cgtgcaatat acggcaatca cagtgttgtt cgtcatatgt ccggatggaa atgatggccc
     8461 cgatcggttc cgatcggatt gatcggaaaa acggatagta gtttttttaa ataaagtcat
     8521 gaatttcacg tgcgttcttt gcatttcgac catccgcaca ctttccacgt gcttgggccg
     8581 atgccgatcg actgccggct tcaatatgtc tgtaaacaac accgcaatca gcaaggacag
     8641 aaccaggcat accacgaaag cccggccctt aaatccgcca aacaagagtg cgctcagccc
     8701 gatcccaatg aacaacggct tccaaatctc gctgttgctg agtgccgcca tgaacaagtc
     8761 gagcgccgga ctcgtccatc gttcgttgac aagatgaaag agggtctggt ccatgtgatg
     8821 gaaatgaagc ggcggtttaa gctttaatcg ctaccttcca atagacaaat gcggtaaatg
     8881 acgaagcacg aatgacgaat gccgaaacaa gctcgaatgc tcaaatgacg aaagtgcgat
     8941 ccggggcctt ctttttgtca ctcgggtttc gtcattcctt cgtcattcgg cattcgagct
     9001 tcgtcatttg ttcgggccta gcgttgtcga tccacgccca gcccgtgcct tccgcgcagg
     9061 acattctcgc ttcggttcga atgatggagg cgcaacagca actcgatctg cagggtcagc
     9121 ttcgccaaaa tgatgtcatt atcccctttc gcctgaccca aaacgggcca ctgattcgct
     9181 attcattcac taacccagac caggttttgc aattgcgcct tggggaaaaa agctcgcgcc
     9241 ttgatcttgt gacgggcgca ggaacggaga aatttgcggc tccgaagctc aaccagaaaa
     9301 tccgcggtac gagcgttagc tacgaagatc tcgcattcaa atttctctac tggccagcgg
     9361 cgcgtgtttt gggcgaagaa aatgtccgga ctcgcaattg ctggaagctg caattgcgtg
     9421 ccccttcgcg tgagtcgcag tattccaatg ttctcctatg ggtcgacaag gcgagcggcg
     9481 cccttatgaa gatggagggc tacgactgga atgcccagct tgcgaaacgc tttgaagtcg
     9541 tctccggaca aaagatcgga agtcgctggt tcctgaaaca aatgcgcatc gaagaacttc
     9601 agcccggcac cactcacgca ctgtcgcgcg cgtatttgga aattaagaaa tgatctgtag
     9661 ccaccgccct atgggcggtt aaaaccgaaa cggccacaga tcaacggccc acagggccgt
     9721 ggctacatca atccggccgc gcggccttca cgatctgcgc gaagctacgc ggatcgaggc
     9781 tggccccgcc aaccaaggcg ccgtcgatgt cgggctggct cattagctcg tgcgcgttct
     9841 ccggtttcac gctgccgcca tactgaatgc gcacgcgctc ggcggtagcg tcgttggcca
     9901 tgtcgcgcag ggtctcacga atatacatgt gcgcttcctg ggcctgctct ggtgtagcgt
     9961 tgcggccggt gccgatcgcc cacaccggtt cgtaggcaat taccgtttcc tgaagttcct
    10021 tttcactcag atccttgaga ctgccgcgta gttggatcgc cagtatcttt tcgacattcc
    10081 ccttatcgcg ctgatcgagc gtttccccga tgcaaacgat cgggcgcagt gttgcttcat
    10141 gcgcggcgcg cactttgagg ttcacaatct catccgtttc accaaagagt atacgacgtt
    10201 cgctgtgccc gagcacgacg tagtgcacaa ataaatcgcg caacagcgct gcgctgatct
    10261 cgcccgtaaa agcaccgttc cgctcccaat gcatgttttg cgcgccgacc ttgatatttt
    10321 gcgctttgtc gagggcttcc atcactctgg caatcgcggt gaacggcggg acgatcacca
    10381 cctcgatatc ggtgatgtcg cctagatcca gcagcagcga ctcaacaaat cgcgccgact
    10441 cggcctgcgt catgttcatc ttccagttcg cagcgacgat tttttttctc ataggcgatc
    10501 aattgaatgg gagcatatgc gatcaattct ccatcactcc attactccac ctccgcgtca
    10561 ctccttctct gaaagcgctg cgacgcccgg cagttccttg ccttcgagta attccagcga
    10621 cgcgccgcca ccggtggaga tgaatgtcat tttatcggca agtcccgcct gtttcaccgc
    10681 ggtcacggaa tcgccgccgc caatgatcgt ggtcgcgcct gattgcgcca gcgcttccgc
    10741 gatcgcaatt gttccctccg aaaaatccgg aatttcaaaa atgccaacag ggccattcca
    10801 cagaatcgtt tcgccttttg cgatttcctc ctggtaaagc gcgatcgtcg ccgcgccgat
    10861 atcgaccgcc tgccatccgt cggtgatccc gtgctgcgac gagagccggc tggtgttttg
    10921 aatcgccgcg ccagtgtgaa tttcctccgc ctccacaacg tcaatcggaa gcaaaaattt
    10981 cacgccccgt tttttagcca ggtcgagaat ttcacgcgcc acatcgacct tgtcggattc
    11041 gacccgactc gcgccgaccg ggattccctg cgctttcaga aaagtattgg ccatggcgcc
    11101 gccgatcagg atcgtgtcgg ctttctccat tagcgctttt aaaacgccga tcttgtcgga
    11161 aacctttgcg ccgcccaaaa tgacgacaaa cggcttggtt ggtttatcga gcccgtcgcg
    11221 caaatgtttc agctcttttt ccatcaggaa acccatcgcc gatttcgcca cgaattttgt
    11281 gatccccact gtcgaggcat gagcgcggtg cgccgcgcca aacgcgtcgt tcacataaag
    11341 atcgccgagt ttagccagcg cttccgcaaa tttcggatcg ttcgcttctt cctccggctg
    11401 gaagcggagg ttctcaagca aggcgacatc gccattctcc atatggttaa tgattttttc
    11461 cggcacgtcc ccaatggtgt cgtggctaaa aatgaccggt tgatggataa gcgagtgcaa
    11521 ataatcgccg atcgggcgaa gtgaatattt ttggacacgc ttgcctttgg gccggccaaa
    11581 atgcgacatc aaaatggtct tcgcgccatg ctcgcggagg taattgatgg tcggcagact
    11641 ctcgcgaatg cgcgtgtcgt cggtaatgtg gacctttcca cccctcgtat cggttggcac
    11701 gttgaaatcc acccgcacca gcacgcgtct accgcgcaca tccagatcgc gaagggtaag
    11761 cttcattgcc gctggagtaa tggagtgtgg gagtgctgga gtggtgtcac gaaattcatt
    11821 actccactac tccatcactc cagttctccc atttcatctg ccgagcagct ccatcaattc
    11881 tacgcaacgg gtgctgtaac cccattcgtt gtcgtaccag ccgaccactt ttacgaagcg
    11941 attgccgagc ataagagtca atttgctgtc aaaaatgcag gagtgcggat tttccacaat
    12001 gtcctgggaa acgagtggct cgtcgctgta ttcgaccacg cctttgtaac ttttgtctga
    12061 cgcagcttct ttgaaggcgg cgttaaccgt atcaactgtc gcatcttttt ccaggacggc
    12121 ggtgaaatca gtaacggaac catccggggt cggcacgcga aacgcgccac catttaattt
    12181 ccctttcaat tccggaatga cttcgccgat cgctttggcc gcgcccgtgg tggatggaat
    12241 aatgttgatc gccgccgccc gtgcgcgcct caaatcttta tgcggaaaat cgaggatgcg
    12301 ctggtcgttg gtgtaactgt gaatggtgct catgaacccg tgcgcaatgc cgaatttatc
    12361 gttcaggacc ttcgccaacg gcgcgagaca attggttgtg caacttgcgt tcgaaacgat
    12421 gtgatgcttt gccggatcat aaagttgatc gtttacgccc atgcaaatcg ttacgtccgg
    12481 atcttttgcc ggtgcgctaa tcagcactcg cttcgcgcct gccttgctca aatgcagctc
    12541 ggctttatcg cggctggtga acaagccggt cgattcgagc acgacgtcga tgtccagttt
    12601 tttccacggc aggttcgccg ggtcgcgctc cttcaaaacc ctgatcttgt ggccccggac
    12661 gatgatgttt tcctcatcgt aaccaatctc gccgtcgaat ttgccgtgga ccgaatccca
    12721 tttgagcaga tgggccagcg tctgcgtgtc agtaagatcg ttaatcgcgc gaacctgctc
    12781 gacttgcttt tgcgtcatgg cgcggagcac gttgcgcccg atgcgaccga aaccgttgat
    12841 agcgtaattt ggcataggaa aattgaatag ttgaagagtt gaatcgttaa accgttaaac
    12901 gtcgaacatc aaacatgaaa ctcggatttg cagtgatcaa gagcggtctc attctggttt
    12961 gcctcgcatt accgatcgtg tttgccgagt tcgcctcgac ctcgccaatc gatgccggag
    13021 acatgaaaag gatcctcgtg ctgggcgaca gcctttcgga cggatttggc ctcaaacgga
    13081 gcgaagccta tccagctttg ctggcggaca aattgcgcgc ggccggcctg aactttcaag
    13141 tgacgaacgc aagccagact ggcggcacca ccgacggcgg cttggaacgg ctcccggccc
    13201 atttgaaacc caagatcgac atcttcattc tagagctggg aatcaacgat gcctttcgcg
    13261 gcatgccggt cgatcaaatc caaaacaatc tccaggagat catcgataag gtgaaagcgc
    13321 gaaatccgaa cgtgcgcgtg gtgattgcgg ggatgcagct gcctaattac ggagcggacg
    13381 actatgtttc tgctttcggc aaattgttcg ctgaactcgc ggccaaaaac ggtgcggctc
    13441 tggttccgta cctccttgac ggcgtggcag gcgatcc
//
LOCUS       DASQUQ010000111         5832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_298052, whole genome shotgun sequence.
ACCESSION   DASQUQ010000111 DASQUQ010000000
VERSION     DASQUQ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298052"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VE486_03175"
     CDS             <1..777
                     /locus_tag="VE486_03175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA38119.1"
                     /translation="ISIVISSRNDPESLERCIESLASKTSYPSYEIVIVEDDSQSPNA
                     RHSPSRLPHRLLRFSGAPGISARRNFAIKQTAAPWLLFLDYNVEVVEPDWLTIMAEHI
                     QRPEVGAVGAQLLNPDGTIAHAGIVLGGNRIARPASGGFPAEHPGANRQLQITRNCSA
                     VSGACMLTRREVFEELGGFDESLPESLADIDLCLKMRRAGYLIVYTPFAKLYWQARQD
                     DRIDPRGDAIMRERWPEVLQRDPYYNPNLSRERADFSLGK"
     gene            796..1542
                     /locus_tag="VE486_03180"
     CDS             796..1542
                     /locus_tag="VE486_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38120.1"
                     /translation="MSKRGKDLWHGIDADAQNALSTRNYRSASFAQELSERGVDASEL
                     AAADRSQREIKEAWIPGAEIFLRKIHPQRYRGFFGEFVRRDEGILAQIGFWPKQWSAA
                     RMFAGTAKGFHVHPPSIPADATAAEWLRRLFVDEPENYSLRRYDDEQWDVMFLLQGRA
                     EMILCDIRAGFPKRIMRFFIDGDNHRSNNNVGVVIPPGVAHAIRAEGSEDVIMVYGTS
                     TIFHPEFEGRIGSEVETAELPQGWEKFLSL"
     gene            1606..2295
                     /locus_tag="VE486_03185"
     CDS             1606..2295
                     /locus_tag="VE486_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZA38121.1"
                     /translation="MKRQFDPAVLEMMDRSQPVSDELERDLKRIRQLNRWFGSYRLIG
                     HFVRRWIKPGDQMRIVDLATGSGDIPRLIVDYARKIGAKVEIDALDRQSATLEIARKL
                     SAAYPEISYHETNILDWSPSEACDIVLCTLVLHHFSDGDAVNVLRRCRESSRDFVLVS
                     DLRRGFLATVGVYLLTALVFREPMTRYDARLSAERAFSFSEMRKLAVRAGWQDFGHRR
                     FRVARQAIWLE"
     gene            2405..3799
                     /locus_tag="VE486_03190"
     CDS             2405..3799
                     /locus_tag="VE486_03190"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HZA38122.1"
                     /translation="MPLLKPDPTFYPSPRLAMQAPPEKHAFVAALSPPGSKLNDALLV
                     VDVDSGSRTYGQRIGEVKMPEFGDELHHFGWNACSSALCPYAPHPHVERRYLVVPGLR
                     SSRIHIIDTKPNPRKPKVVKVIEPEEVFAHSNYSRPHTIHCGPEGIYVSALGAPNGDG
                     PGGIFMLDCETFELLGQWEMNRGPQFLHYDFWWHLGFDTLLTSEWGTPNMIENGVQPD
                     LLLGGKYGHQMHIWDLRRRRHLQSLDLGKEQQMVLEMRPAHNPTKAYGFVGVVVSLKD
                     LSASVWLWHRNNGAWDLKKVIEIPAEPADPAKLPPLLKDFKAVPPLVTDINLSLDDKF
                     LYVSCFGTGEFLQYDVSDPFSPKKTGSVRIGGIANRTPHPKNPEQPLAGGPQMVEVSR
                     DGKRVYFTNSLYAAWDEQFYPDGVGSWMVKLNAGQEGGLSFDENFFVESTDYRVHQVR
                     LEGGDSSSDSFCFP"
     gene            3892..4467
                     /locus_tag="VE486_03195"
     CDS             3892..4467
                     /locus_tag="VE486_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38123.1"
                     /translation="MQEQKRAAVLRALPPIVLGHALSIGIVIAAVLVARISLPPLPLK
                     IGSAAVLFAFGLYRLFRSRHPNWVGMRVGFADLTLWSFVMASAHGAGLMLVPLFIKTP
                     SQTPAPHGGGAMALHTHATHGFEFANLSTPALLTSSIAIHTLGYLLVTGLVAIVVYER
                     LGVGILRRAWFNVDVFWMLALMIAGLFIFFL"
     gene            4518..5354
                     /gene="panC"
                     /locus_tag="VE486_03200"
     CDS             4518..5354
                     /gene="panC"
                     /locus_tag="VE486_03200"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZA38124.1"
                     /translation="MKIVTTIAKVHSRQRSKPSVLVPTMGALHKAHLELIRVARERVG
                     KDGEVAVSIFVNPLQFEPGSDYERYPRPEKADEEFCRNARVDLLFRPPAEELYLKDRS
                     TYVEETSLSSVLEGESRPGHFRGVCTVVAKLFNILAPDAAIFGEKDFQQLAIVRRMVR
                     DLNFKIDIIAVPTVREEDGLACSSRNQYLTAEERKQAMILRKALVKGADMARAGEKSA
                     ARIVSVVRETIGQAPLARIDHVDLVDADRFQPLEFVRPNSLLALAVFFGQTRLIDNIL
                     LV"
     gene            5351..>5832
                     /locus_tag="VE486_03205"
     CDS             5351..>5832
                     /locus_tag="VE486_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZA38125.1"
                     /translation="MKEYDFVVIGSGIAGLSFALEAATHGSVAVITKRKGADTNTAWA
                     QGGIACVTSDEDSFELHVRDTIEAGAGLCDEAVVRTMVTEGPERIGDLIELGLHFDER
                     EVSGHLELDLGKEGGHSKRRVLHVRDVTGKEIEETLLREIGRRPEIELLENHMAVDLI
                     T"
BASE COUNT         1225 a         1524 c         1706 g         1377 t
ORIGIN      
        1 atttcaatcg tcatttcgag ccgcaacgat ccggagtcac tcgagcggtg tattgaaagc
       61 ctggcaagca agacgagtta cccgagctac gaaatcgtca tcgtcgaaga tgacagccaa
      121 tcccctaacg cgcgccattc cccctcgcgg ttgccacatc gtttgttgcg cttctcgggc
      181 gcacccggta tctccgcgcg caggaatttc gcaatcaaac agaccgctgc tccatggctt
      241 ctctttctgg actacaatgt cgaagtcgtc gagccggatt ggctaacgat catggctgag
      301 catatccagc gtccggaagt tggcgcagtg ggcgcgcaat tgctgaatcc agacggcacg
      361 attgctcatg ccgggattgt tctgggcggc aatcgaatcg cgcggccggc ttccgggggt
      421 tttccggcag aacaccctgg agcgaatcgc cagttgcaga ttacgcgtaa ttgcagcgcc
      481 gtgtcgggcg cgtgcatgtt gacgcgccgc gaagtatttg aggaattggg cgggttcgat
      541 gaaagcctgc cggaatcgct tgccgatatc gatctttgcc tcaaaatgcg ccgggcgggt
      601 tatctgattg tttacacccc atttgcaaaa ctctattggc aggcacggca agatgacagg
      661 atcgacccgc gcggcgacgc aatcatgcgc gagcgctggc ccgaggtttt gcagcgcgat
      721 ccctattaca atccaaatct ttctcgcgag cgcgctgatt tctcgttagg caaataaatc
      781 ccaaattgaa ttaaaatgag caagcgcgga aaggatctct ggcacggcat tgacgctgat
      841 gcacaaaacg cactaagcac tcgtaattat cgctcggcgt catttgcgca ggaactgtcg
      901 gagcgaggcg tggatgcgag cgaactcgcc gcagcggacc ggagccagcg cgaaattaag
      961 gaggcttgga ttcctggggc ggaaattttt ctacgaaaaa ttcatccgca gcgctaccgc
     1021 ggattctttg gcgaatttgt ccggcgcgac gagggcattc tcgcgcagat cggtttctgg
     1081 ccgaagcaat ggtcggcggc gcggatgttt gcggggaccg ccaaaggctt tcatgtgcat
     1141 ccgcccagca ttccagcgga cgcgactgca gcggagtggt tgcgccggtt gtttgtcgat
     1201 gaaccggaga attattcgtt gcgccgttac gacgacgagc aatgggacgt gatgtttttg
     1261 cttcagggtc gcgcagaaat gatcctgtgc gatatccgcg ccggatttcc caaacgcatc
     1321 atgcgctttt ttatcgatgg cgacaatcat cgcagcaaca acaacgtcgg cgtcgtcatt
     1381 ccgcccgggg tagcgcatgc cattcgcgcg gaaggttccg aagacgtgat catggtttac
     1441 ggcacgagca cgattttcca tccggaattc gaggggcgga ttggaagcga agtcgagacc
     1501 gcagagttac cacaagggtg ggaaaaattc ctctctttgt agcgggcgct ctgttagcgt
     1561 cagacgaatt aatgcggcgg tcatggaccg ccgctacagt aacgaatgaa gcggcaattc
     1621 gatccggctg ttttagaaat gatggatcgg tcgcagccgg tttctgacga gctagaacgc
     1681 gatctgaaac ggattcgcca gctcaatcgc tggtttggca gctatcgttt gattggccat
     1741 tttgtgcgcc gttggatcaa gcccggtgac cagatgcgca ttgtcgatct tgccactgga
     1801 tcgggcgaca ttccgcgatt gattgtcgat tatgcacgaa aaattggcgc gaaagttgag
     1861 atcgatgcgt tggatcgaca atccgctacc ctagagatcg cgagaaaact aagcgccgct
     1921 tatcctgaga tttcgtacca cgaaacaaac attctcgatt ggagtccgtc ggaagcctgt
     1981 gacatcgtgc tttgcacgct cgtgctccat catttcagcg acggcgacgc cgtgaatgtt
     2041 ctgcgacggt gccgcgagtc atctcgcgat tttgttctgg tttccgattt gcggcgcgga
     2101 tttttggcca cggttggagt gtatcttttg accgcactcg tttttcgcga gccaatgacg
     2161 cgctacgatg cgcggctttc agccgaacgc gcattttcat tttcagaaat gcgcaaactc
     2221 gccgtgcgcg ccggctggca agatttcgga cacaggagat ttcgggtcgc gcgccaggcc
     2281 atttggctcg aatgaaatta gggttgcatt gaccttgtct gcggtttgga atgagtgaga
     2341 acgatatctt gaagtttccc gtgtgaagac gtaatgaagt gaaccataga acgggcagga
     2401 atttatgccg ctcttgaagc cagacccgac gttttacccg tcgccgcgat tggcgatgca
     2461 agctccgccg gagaagcatg catttgtggc ggcgctaagt cctcccggca gcaagctcaa
     2521 cgacgcgctg ttagttgtcg atgttgattc cgggtcgcgc acctacggtc agcgcatcgg
     2581 cgaggtcaaa atgccggagt ttggcgacga gctacatcac tttggctgga acgcatgcag
     2641 ttcggccctt tgtccgtatg caccacatcc gcacgtcgaa cgccgttacc tggttgttcc
     2701 tggcctgcgc tcatctcgta ttcacattat cgacaccaag cctaatccgc gaaaaccgaa
     2761 ggtggtcaaa gtgatcgagc cggaagaagt ttttgcgcac tctaattatt cgcgtccgca
     2821 cacgattcac tgtgggccgg aaggcattta tgttagcgcg cttggtgcgc ccaatggtga
     2881 cggcccgggc ggcattttca tgctcgattg cgaaacgttc gaactcctgg gtcaatggga
     2941 gatgaaccga ggtccacaat tcttgcatta tgatttttgg tggcatttgg ggttcgacac
     3001 gctgctgacc agcgaatggg gtacgccgaa catgatcgaa aacggagtgc aaccagacct
     3061 gctccttggc ggtaaatacg gccatcaaat gcacatttgg gacctgcgcc gccgccgcca
     3121 tttgcagtct ctcgatttgg gcaaggagca acaaatggtt ctcgagatgc gccccgcaca
     3181 caatccgacg aaagcgtacg gtttcgtcgg cgtcgtcgtt tcgctgaagg atttgtccgc
     3241 gtctgtctgg ctctggcatc gcaataacgg cgcgtgggac ttaaagaaag taatcgaaat
     3301 tccagctgaa cccgccgatc cggcgaagtt accaccgttg ctaaaggatt ttaaagcagt
     3361 gccgccgcta gtgacagaca tcaatttatc gctcgacgac aaattcctct acgtttcctg
     3421 cttcggaaca ggggagttcc tgcagtacga cgtgtccgac ccattcagcc caaaaaagac
     3481 tggctccgtg cggattggcg ggattgctaa ccgaacgcct cacccaaaga atccggagca
     3541 accgctcgcc ggcggaccgc aaatggtgga agtaagccgc gatggcaaac gggtctattt
     3601 cacgaattca ctttacgctg cgtgggacga acaattctat ccggacggcg tcggcagttg
     3661 gatggtgaaa cttaacgccg gccaggaagg cggcctttcc ttcgacgaaa actttttcgt
     3721 cgagagcacc gattatcggg ttcatcaagt ccgcctcgaa ggcggcgatt cctcaagcga
     3781 ttctttctgt tttccgtgag cgggctctgg ccgtggctga cgctgttcgg gctcggcgct
     3841 tttcacggga tcaatccagc aatgggatgg ttgttcgctg ttggactggg gttgcaggaa
     3901 cagaagcgtg cagccgtcct tcgtgcgctg ccgccgattg ttctcgggca cgctctttcg
     3961 attgggattg ttatcgcggc ggttctcgtg gctcgcatta gtctgccgcc cttgcctcta
     4021 aagattggct ctgcagcggt cctattcgct tttggattgt atcgcctttt tcgctcacgt
     4081 cacccgaact gggtaggcat gcgcgtgggt tttgccgatt tgactctttg gtcttttgtg
     4141 atggcgtcgg cccatggcgc gggtttgatg ctggttcctt tatttattaa aacaccttca
     4201 cagacgcctg caccacacgg ggggggtgca atggccctgc acacgcacgc cacccacggc
     4261 ttcgaattcg ctaacttgag cacgcctgct ttgctgacca gttcaattgc aatccatacc
     4321 ttgggctatc ttctggtcac cggcctggtc gcaatcgtgg tttacgaaag actcggggtc
     4381 ggcatcttgc gccgagcctg gttcaacgtc gacgtgttct ggatgctcgc cctgatgatc
     4441 gctggcttat ttatcttttt cctttgaatt gaagccggag attcgcgcgc tcgatgcgat
     4501 tgagtgataa tcggtgcgtg aaaattgtga ccacgatcgc gaaggtccat tcgcgacagc
     4561 gctcaaaacc gagcgtgctc gtgcccacga tgggagcgct gcacaaggcg catctggaat
     4621 tgattcgggt tgcgcgtgag cgagtcggaa aagatggcga agtggcggtg agcatctttg
     4681 tgaacccact tcagtttgag ccgggcagcg attacgaacg ctatcctcgt cccgaaaaag
     4741 ccgatgaaga attttgccga aacgcaaggg ttgatctttt gtttcgaccc ccggccgagg
     4801 aactgtattt aaaagaccgt tcgacctacg tagaggaaac ttcgttgtcg agtgttctcg
     4861 agggcgaatc gcggccgggg catttccgcg gcgtgtgcac agtggtggca aagcttttta
     4921 atattcttgc cccggacgcg gcgatctttg gggaaaagga ttttcagcag ctcgcaatcg
     4981 ttcggcggat ggtgcgcgat ctcaatttca agatcgacat tattgctgtg ccaactgttc
     5041 gcgaagagga cgggctggcg tgtagttcgc gcaatcagta tctcaccgcc gaggagcgaa
     5101 aacaggcaat gattttgcga aaggctttgg tgaagggagc cgacatggct cgtgctgggg
     5161 aaaagtcggc tgcgcgaatt gtctcggtag ttcgcgagac gattgggcag gcgcccctcg
     5221 ctcgaattga tcacgtcgat cttgtcgacg cggatagatt ccaaccgctc gaattcgtca
     5281 ggcccaattc gttgctcgcg cttgccgttt tcttcggcca aactcgtttg atcgacaaca
     5341 ttctgcttgt gtgaaggaat acgatttcgt tgtgatcggc agcggcatag ccggtcttag
     5401 ctttgcactg gaagcggcaa cacatggttc ggtggccgta atcacgaaac gcaaaggcgc
     5461 cgacacaaat acggcctggg ctcagggtgg gatcgcgtgc gtgaccagcg acgaagattc
     5521 attcgagttg cacgtgcgcg acaccatcga ggcaggcgcc ggactttgcg atgaggcggt
     5581 ggtccgcacc atggttactg aaggtcccga gcgtatcggc gacctgatcg aactgggatt
     5641 gcacttcgat gagcgcgaag tctccgggca tcttgagctt gatctgggca aagagggcgg
     5701 tcactcgaag cgtcgggtcc tgcatgtgcg ggacgtgacc ggaaaggaga tcgaggagac
     5761 tttgctgcgc gagatcggcc ggcgaccgga gatcgagctt ttagaaaatc acatggcggt
     5821 tgatttgatc ac
//
LOCUS       DASQUQ010000112         4548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_110823, whole genome shotgun sequence.
ACCESSION   DASQUQ010000112 DASQUQ010000000
VERSION     DASQUQ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110823"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="VE486_03210"
     CDS             complement(<1..1480)
                     /locus_tag="VE486_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZA38126.1"
                     /translation="MAKANTKIEDYAFLSDTQTGALVSRDGCVDWLCFPRFDSPACFA
                     SLLGGKKNGHWRFFPEEKIEQTRRQYRDETLILETEIETKNGAVRLIDFMPPRGENPD
                     IIRIVEGLRGEVRMQMELIIRFDYGQIVPWVRKCHGGLEAIAGPDGLILRTPIETRGE
                     DLTTVAEFTVAKGDRVPFVLTWFLSHRDPPRKVNAEHALRDTEKYWREWSNQCVIKGE
                     WRDAIVRSLVTLKGLTYAPTGGLVAALTTSLPEEIGGVRNWDYRYCWLRDAAFTLLTL
                     VGAGYLEEAKSWREWLLRAIAGSPAQMQAIYGVRGERRLDEYEIPWLSGYEDSKPVRI
                     GNAASNQFQLDVYGEILAAMWLADRAGIKMEEPDWALMVQLMKFLESNWQKPDEGMWE
                     VRGGRKQFTHSKMMAWVAFDRAVKLVEECGCAADEHLERWQKIRDQIHAEVCERGYNI
                     EKKAFTQHFGSDAMDASLLMMPLVGFLPITDERVRSTIEAIER"
     gene            complement(1495..2010)
                     /locus_tag="VE486_03215"
     CDS             complement(1495..2010)
                     /locus_tag="VE486_03215"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZA38127.1"
                     /translation="MKTATDFEIRPARVEDVPIILELIRDLATYERAPNEVTATEEQL
                     VDVLFGTRPAAEVLLAFEGKSPVGFAVFFHNFSTWLGRPGLYLEDLFVKPDKRGKGYG
                     RALLVDLAKIARDRGCGRMEWAVLDWNEPAIKFYRALGAKPMDEWTVFRLTRDGIARL
                     AQAKKADQEHE"
     gene            complement(2123..3211)
                     /locus_tag="VE486_03220"
     CDS             complement(2123..3211)
                     /locus_tag="VE486_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZA38128.1"
                     /translation="MRHKHSDYMHWSKTQSRARFNLATSGVASFPLRELPVNLETLEI
                     NGDNSYGHAPLQRAIATHHGVDPDCVVESAGTSMANHLAMAAILEPGDEVLIEHPTYG
                     PILDVAQYLHANVKRFPRIKETGWTIDVGAILHNITPKTRLIVITNLHNPTSVLTPDS
                     VLREIGDIARSIGAFVLVDEVYLDAVYQRTPRTSFHLGPEFVVTSSLTKVYGVSGLRC
                     GWILARPDLAWKMRRLNDLYSATPVHPGELLSVAAFKHLNLLRDRARRIVEADRKLLH
                     EFLKKQEEVSAVATDWGTTSFVRLREGNVDTFLDRLRSDFDTSAVPGRFFEMPDHFRI
                     GMGVNTEMFAEGLNRIGRALVLATTGKP"
     gene            complement(3208..>4548)
                     /locus_tag="VE486_03225"
     CDS             complement(3208..>4548)
                     /locus_tag="VE486_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZA38129.1"
                     /translation="FADMTVLDNSKPRRQLRLFDATMIVMGGIVGAGIFANPSEVAHR
                     VHTPFLILGVWVLGGFIAMCGAFIWAELATRLPGTGGQYLYLREAYHPSVAFVYGWGL
                     LLVTQTGGMAAVAVIFSSYFRALTGVVWNDSAIAAVVLLILTGINCLGARAGSNVQST
                     LMLLKIAALAALVILGFAFGGGSLKSGPLLAQPISLGLLKDIGSAMVPIAFAYGGWQT
                     ATFVAGEMRDARRDLSRGLLMGVAAVVGLYLAVNLACLCVLGPAGLDATTTPASDVMR
                     IALGERGAQWIAAGIAVSTLGFLSQSMLTAPRVYYAMARDGLFFRSVGKLSRHSRAPV
                     VAIILQGVAATVIACSGTYGEILNFEVTVDFIFFGMTAASLFILRRRQIGSDAVIYRV
                     PGHPFTTILFVLSCAGIVGSAIVASPANSAIALGIMLAGLPVYYYWSRFRRARS"
BASE COUNT         1025 a         1373 c         1206 g          944 t
ORIGIN      
        1 cgcgttcaat cgcctcaatt gtgctacgca cccgttcgtc agtaattgga aggaatccga
       61 ccagcggcat cataaggaga ctggcgtcca tcgcatctga cccgaagtgc tgggtaaatg
      121 ctttcttctc gatattgtag ccgcgctcgc aaacttccgc gtgaatttga tcgcggattt
      181 tttgccagcg ctcgagatgt tcgtcagccg cgcagccaca ttcctcaaca agcttaacgg
      241 cgcgatcaaa cgccacccag gccatcattt tggaatgggt aaactgctta cgtccgccgc
      301 gcacttccca cattccttcg tccggtttct gccagtttga ttccaggaat ttcatcagct
      361 ggaccatcaa agcccaatcc ggctcttcca ttttaattcc cgcacgatcg gccagccaca
      421 tcgcagccaa tatttcgccg taaacatcga gctgaaattg gttcgatgca gcgttgccga
      481 tacggaccgg cttcgagtct tcgtagccgg acagccatgg gatttcgtac tcgtcgagtc
      541 tgcgttcgcc gcggacgcca tagatcgcct gcatttgtgc cgggctcccg gcaatggcgc
      601 gcagaagcca ttcgcgccac gatttggcct cctcgagata gcctgcgccc accagggtaa
      661 gcagggtaaa tgcggcatct ctcagccaac agtagcgata atcccagttg cgtacgcccc
      721 ctatttcctc cggcaacgag gtggtgagcg cagcaaccag gcccccggtc ggcgcgtagg
      781 tcaatccctt aagcgtgaca agcgagcgca caatcgcgtc gcgccattct cctttgatta
      841 cacattgatt cgaccattct cgccaatatt tctcggtgtc acgcagtgcg tgctcagcat
      901 tgacttttct gggcgggtcc ctgtgcgaca agaaccaggt aagcacaaat gggacgcgat
      961 ctcctttcgc caccgtaaac tccgcgacag tagttaaatc ttcgccgcgt gtttcgatcg
     1021 gcgtccgcaa aatcaaacca tcaggtccgg cgatcgcttc cagtccgccg tgacatttgc
     1081 gcacccatgg aacgatctgc ccataatcaa accgaatgat cagctccatc tgcatgcgta
     1141 cttcgccgcg caagccttca acgatccgaa tgatgtcggg attctcaccg cgcggaggca
     1201 tgaaatcgat caggcgcacg gcgccgtttt tcgtttcaat ctccgtttcc agaatgagcg
     1261 tctcgtctcg atactggcga cgcgtctgct cgatcttttc ttcggggaaa aatcgccagt
     1321 ggccgttttt cttgccaccg agcagcgagg caaaacatgc tggcgaatcg aaccgcggaa
     1381 aacaaagcca atcgacacac ccgtcgcgac tcaccagcgc acccgtctgc gtgtcactga
     1441 gaaacgcgta gtcctcgatc tttgtattcg ccttcgccac tttgattccc cctcttactc
     1501 gtgctcctga tcggctttct ttgcctgtgc gagcctggcg attccatcgc gcgtcagtcg
     1561 gaacacggtc cattcgtcca tcggttttgc cccaagggcg cggtagaatt tgatggcggg
     1621 ctcgttccaa tcgagcaccg cccattccat ccggccgcag ccgcgatcgc gcgcaatctt
     1681 cgcaagatcg acaagtaatg cgcggccata acctttgccg cgcttatccg gcttcacgaa
     1741 caggtcctcg agataaagcc caggtcgccc aagccatgtc gaaaaattat gaaaaaacac
     1801 agcaaagccg accggcgatt ttccttcaaa cgcgagcaga acttccgcgg cgggtcgcgt
     1861 cccgaataaa acatcgacca gttgttcttc cgtcgcggtg acttcgtttg gcgcgcgctc
     1921 gtaggtcgcc aaatcacgaa ttaattccag aatgatcggt acgtcttcca cacgcgccgg
     1981 acgaatctcg aaatcggtgg ccgttttcat tagctgctga ctatcgggtt cccggagaaa
     2041 cgttcaacgc ccaacgtcta acgtctacgt cgaatggaga aagtttggca ttggagcgtt
     2101 cgatgttcca acctttgcgg catcacggtt tgcctgtcgt cgcgagtaca agcgcgcggc
     2161 cgatgcggtt caggccttca gcgaacattt cggtgttcac acccatgccg atccggaagt
     2221 gatcgggcat ctcgaagaac cggcctggga ccgccgaagt atcgaaatcg gatcgcaacc
     2281 gatccaggaa agtatcgacg ttgccctctc gcaatcgcac gaatgaagtt gtgccccaat
     2341 cggttgctac ggcggagacc tcctcctgct ttttgagaaa ctcgtggagc aatttgcgat
     2401 ccgcttccac gatccggcgc gctctgtcgc gaagaagatt cagatgcttg aaggcggcga
     2461 cgctgagaag ctcgcctgga tgaaccggtg tcgcgctata gagatcgttc aggcgtcgca
     2521 tcttccaggc gagatccgga cgcgccagga tccagccgca gcgcagtccg ctgacgccgt
     2581 aaaccttcgt caaactgctg gtcaccacaa actctggccc gagatgaaac gacgttcgcg
     2641 gggtccgctg gtaaaccgca tccagataaa cttcgtccac cagcacgaat gctccaatgc
     2701 tgcgcgcgat gtcgccaatt tcgcgtagaa ccgaatccgg cgtaagcacg ctcgttgggt
     2761 tgtgcagatt tgtgatcacg atgagtcgtg tcttcggcgt gatgttatga agaattgctc
     2821 ccacatcgat ggtccagccg gtttccttga tgcgcggaaa ccgtttcacg ttcgcgtgga
     2881 gatattgggc aacatctaga atcggtccgt acgttggatg ctcgattaaa acctcatcgc
     2941 ccggttcgag gatcgcggcc atggcgagat gatttgccat cgacgtgccc gcggattcca
     3001 caacgcaatc ggggtcaacg ccgtggtgcg tcgcgatagc tctttgtaag ggtgcgtggc
     3061 cgtagctatt gtcaccattg atttcaagtg tctccaaatt gactggcaac tcgcggagtg
     3121 ggaacgacgc caccccactt gtggcgagat tgaaccgggc gcggctctgc gtcttggacc
     3181 aatgcatgta gtcggaatgc ttgtgtctca tgagcgggct cgtctgaatc tgctccaata
     3241 atagtaaacc gggagtccgg ccagcatgat gcccagcgcg atcgcgctat tggcaggcga
     3301 agcaacgatg gcgcttccca caattccggc acaggacaag acaaacagga tcgtggtgaa
     3361 tgggtgtccc ggcacgcggt aaataactgc gtccgatccg atctggcgac gccgcaaaat
     3421 aaagagcgat gccgccgtca ttccgaaaaa gatgaaatca acggttactt caaagtttaa
     3481 gatctcgcca tacgtcccgg agcaggcgat gaccgtcgcc gcgacaccct ggagaatgat
     3541 cgcaaccacc ggtgctctgg aatgtcgcga gagcttgccg acacttcgaa agaaaagccc
     3601 gtcccgcgcc atcgcgtagt acacccgcgg agccgtcaac atgctttggc tgagaaatcc
     3661 gagggtggaa actgcgatgc cagcggcaat ccattgcgct ccgcgttcgc ccagggcgat
     3721 ccgcatcaca tcggaagccg gagtcgttgt tgcatcgagg cctgccggac cgagcacgca
     3781 caggcacgcg agattgactg ccagataaag cccgacgacg gcggccacgc ccatcagcag
     3841 cccgcgcgat aaatcgcggc gcgcgtcgcg catttcaccc gcaacaaaag tggcggtttg
     3901 ccatccgcca taggcaaaag cgatgggaac catggcggat ccaatgtcct tcaagagccc
     3961 gagcgaaatc ggctgtgcaa gcaatggccc tgatttgaga gatccaccgc caaacgcaaa
     4021 tccaagtatt acgagcgccg cgagcgccgc aatctttagc aacataaggg tgctttgcac
     4081 attgctgccg gcgcgcgctc caaggcagtt aatcccagtc aggataagca gaaccacagc
     4141 tgcgattgcg ctgtcgttcc aaaccactcc cgtgagcgcg cgaaaataag atgagaaaat
     4201 aaccgccaca gcggccatcc cgccggtttg tgtgacgagc agcaagcccc atccgtacac
     4261 gaacgcaacg gaaggatgat acgcctcgcg caaatataga tactgaccgc ctgttccggg
     4321 caaacgcgtg gccaactcgg cccagataaa cgcgccacac atcgcgataa agccgcccag
     4381 cacccagacg ccgaggatca agaacggcgt gtgaacacga tgcgcgacct cggagggatt
     4441 cgcgaaaatt cccgcgccca cgatgccgcc catcacgatc atggtcgcgt caaacaatct
     4501 aagctgacgc cgcggtttcg aattatccaa cactgtcatg tcggcgaa
//
LOCUS       DASQUQ010000113         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1301623, whole genome shotgun sequence.
ACCESSION   DASQUQ010000113 DASQUQ010000000
VERSION     DASQUQ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301623"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1021)
                     /gene="mutY"
                     /locus_tag="VE486_03230"
     CDS             complement(35..1021)
                     /gene="mutY"
                     /locus_tag="VE486_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZA38130.1"
                     /translation="MTSPETFRVFRRSLLRWFAHHGRDLPWRHTRDPYAILISEFMLQ
                     QTQVSTVIPYYNEWIRRFPDFATLARASESDVLHAWQGLGYYARARNLHGAAKTVHAR
                     HGGRFPRDLAAIRALPGIGRYTANAVATFAFGQPIPIVETNTARVLARLFDFHSPIDR
                     AAGREELWKRARTLVPKYSACIYNSALLDLGALICSPRQPKCGICPVKKFCRAKEPET
                     LPKRKARLPTLRLTEHHAFIFRQKAILLEQASHRWRGMWILPPLQRRSKNQRPVYRSI
                     FPFTNHRVVLKVYAHRQRKIDKRSQRWIRIDSLDSIPIPSPHRRALQILLHA"
     gene            1118..2065
                     /gene="rnhC"
                     /locus_tag="VE486_03235"
     CDS             1118..2065
                     /gene="rnhC"
                     /locus_tag="VE486_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HZA38131.1"
                     /translation="MNSYTHALTHEQVTRLRVLLEELGFKFSPKEYTLFFAQKNRLSV
                     AVYEKGPKVLVQGRGVEEFVQFELEPKILGEAKLGYEEVHLPEMFEPHFGVDESGKGD
                     FLGPLVIAGVYVDHGIARKLLDSGVQDSKRIGSDARIRALAQTIRKSANGLVETVLIG
                     PQRYNELYEKFGNLNKLLGWGHARVIENLLAKKPDCPRSLSDQFADARVIEQSLLRHG
                     RKIDIDQRTKAESDIAVAAASILAREAFINWLERRGKELGFRLERGASANVKEIAKKL
                     VEMKGPDALREVAKVHFRTAHEVAPNHYAAPVPRENWRK"
     gene            2216..2572
                     /locus_tag="VE486_03240"
     CDS             2216..2572
                     /locus_tag="VE486_03240"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZA38132.1"
                     /translation="MAKLTKRDIVVAISNQTGMVQHQVFDVVQRTLDKITDSLANNIT
                     VELRNFGVFQPRLTKPRVGRNPNQPGSSFVIPPRVTVKFKAGKVMRQRVEKLSRQLKE
                     TAEGREKPPLSGPDSG"
     gene            complement(2810..>3272)
                     /locus_tag="VE486_03245"
     CDS             complement(2810..>3272)
                     /locus_tag="VE486_03245"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38133.1"
                     /translation="VNTCGELQRFSRPELVDLFGKFGVELYDLCRGIDHRPVEPDRPR
                     KSLSTEETFPVDLTTVEQCEEKLEELFQDLMADLAQKESTREIRKIFVKLKFNDFTRT
                     TAERAGLLPTLQDFRSLLAAAFSRTGKPVRLIGLGVRFAETTPESAQMSLL"
BASE COUNT          772 a          803 c          982 g          715 t
ORIGIN      
        1 tagattttgg attttggatt ttggattgcg atggtcaagc atgaaggagg atctgaagcg
       61 cccttcgatg tggcgaagga atcggaattg aatcgagcga gtcaattctg atccagcgct
      121 gcgagcgctt gtcgatcttt cgctggcgat gcgcataaac tttcaacacg acgcgatggt
      181 ttgtgaacgg aaagatcgat ctgtacaccg gacgttgatt tttcgatctc ctttgcaacg
      241 gtggcaagat ccacatgccg cgccagcggt gactggcttg ctcaagtaag atcgcctttt
      301 gcctgaaaat aaacgcgtga tgttccgtta gccgtaatgt cgggagccgc gcttttcttt
      361 tcggaagagt ttccggttcc tttgcgcgac agaatttttt aaccggacaa atcccgcatt
      421 ttggctggcg tggagaacaa atcaacgcgc cgagatcgag cagcgccgaa ttgtaaatgc
      481 aggcggaata ttttggaacc agcgtgcggg cgcgcttcca gagttcttcc cggccagcgg
      541 ctcggtcgat cgggctgtga aaatcaaaca ggcgcgccag gacgcgagca gtgttggtct
      601 cgacaatcgg aattggctgg ccaaaggcga acgtggcgac ggcattggca gtgtaacgcc
      661 cgatcccggg caatgcgcga attgcggcga gatcgcgcgg aaatctgccg ccgtgtcgag
      721 catgcacggt tttagcggct ccatgtagat tgcgcgcccg cgcatagtag ccgagccctt
      781 gccacgcgtg gaggacgtca ctttctgaag cccgagccag cgtggcaaaa tcagggaagc
      841 ggcggatcca ctcattataa taagggatga cagtggacac ctgggtctgc tggagcatga
      901 actcggagat gaggatggcg tagggatcgc gcgtgtgacg ccacggaaga tcgcggccat
      961 ggtgcgcgaa ccagcggaga agagagcggc ggaacacgcg aaaagtttct ggcgatgtca
     1021 cgatttgaaa gcaaacgtcc aatgtccaac gctcaacgtc cacctctcaa ttcagaagat
     1081 gattgaacat tgagcgttga gcgttgagcg ttgggcgttg aattcttaca cccacgcgct
     1141 gacgcacgaa caggtcacaa ggctgcgggt cttgctcgaa gagctcgggt tcaaattttc
     1201 tccaaaggaa tacacgcttt tttttgcgca gaagaacaga ctgagcgtcg cggtttatga
     1261 gaagggaccg aaggtgctcg tgcaaggcag aggcgtggag gagttcgtgc agttcgagtt
     1321 ggagccgaag attctcggtg aagcaaagtt gggttacgaa gaagtccatt tgccggaaat
     1381 gtttgagccg catttcggag tcgatgagag tggaaagggc gattttcttg gccctttggt
     1441 gattgcgggc gtttatgtcg accatggaat tgcccgcaaa cttctcgatt ccggggtaca
     1501 ggacagcaag cgaattggtt cggatgcgcg gatccgcgcg ctcgcccaaa ccattcgcaa
     1561 gagtgcgaac ggtttggtcg aaacagtcct gattggtccg caaagataca atgagcttta
     1621 cgagaagttc ggtaatttga acaaactgct cggttgggga catgcgcggg tgatcgagaa
     1681 tctgctggca aaaaaaccgg attgcccgcg gtcgctttcg gaccagtttg ccgatgcgcg
     1741 ggtgatcgag caatcgctgt tgcggcacgg ccgcaagatc gatatcgacc agcggaccaa
     1801 agcggaatca gatatcgcag tcgcggcggc atcgattctg gcgcgggaag ctttcattaa
     1861 ctggctggag cgccgaggta aagagctggg gttccgactt gagcgggggg catctgcgaa
     1921 cgtaaaagag atcgcgaaaa agctcgtcga aatgaaggga ccggacgcgt tgcgtgaggt
     1981 agcgaaggtg catttccgaa ccgcgcacga ggtcgcgccg aatcattatg cggccccagt
     2041 gccacgcgag aattggcgaa aataggggcg gaacgcctcc acgcaggact gggaaaggtg
     2101 gcaaatcgcc atcgcgtttc gccttcttat ccaagaggtt agcccggcta tttttttgct
     2161 tttgtgctta atctgaccac cgctacgact acatgaagcg attactagat tagatatggc
     2221 aaagctaacc aaacgcgaca tcgttgtcgc catcagcaac caaaccggta tggttcagca
     2281 tcaagtcttt gatgtggtcc agcgtacgct cgataaaatc accgatagtt tggcaaacaa
     2341 tatcaccgtt gaactgcgca acttcggcgt ttttcagccg cgattgacca agccgcgcgt
     2401 cgggcgaaat ccgaatcaac ccggcagcag tttcgtgatt ccgccacgag tgacggtgaa
     2461 atttaaagct ggcaaggtca tgcgtcagcg tgtcgagaaa ctttcgcgcc aactcaagga
     2521 aaccgccgaa ggacgcgaga agcctcccct atccggccct gatagcgggt agccgaaaat
     2581 tctgggctaa aggtttccgc attcgctgaa actatggcgt ggcaagggac agcgaatctg
     2641 tattgcgaca agtagccgat gcgttatttt tttcttcccg caagtgagtg tgcgcaggtg
     2701 caaagttccg tgacaattga tattggcttc cagagcgtct atcactggtt agttggcgct
     2761 gcaaagaccc caatcaacca tgattgcggc tggcaaaggc ggactacaac taaagcaaag
     2821 acatctgagc gctctccggc gtggtttcgg cgaagcggac gccgaggccg atgagacgga
     2881 cgggttttcc ggtgcgggag aatgctgcgg cgagcagtga ccggaaatct tgtaaagtcg
     2941 gcaggagccc ggcgcgctcg gccgttgtgc gagtgaaatc gttgaactta agtttcacga
     3001 aaatcttcct gatctcgcgc gttgactctt tttgcgcgag atcggccatc aaatcctgga
     3061 acaactcttc cagtttctct tcgcattgct cgacggtggt cagatcgacc gggaacgttt
     3121 cctctgtgct gagtgatttg cgcggacgat ccggctcgac tggccggtga tcgatcccgc
     3181 ggcaaagatc atagagctcg acgccaaact tgccgaaaag atcgacaagt tccggacgcg
     3241 aaaagcgctg cagttcgccg caggtgttaa ct
//
LOCUS       DASQUQ010000114         5920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1301938, whole genome shotgun sequence.
ACCESSION   DASQUQ010000114 DASQUQ010000000
VERSION     DASQUQ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301938"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..128
                     /locus_tag="VE486_03250"
     CDS             6..128
                     /locus_tag="VE486_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38134.1"
                     /translation="MNDDNAVDADKMFTILMGDVVEPRRQFIEDNALNVRNLDV"
     gene            137..916
                     /locus_tag="VE486_03255"
     CDS             137..916
                     /locus_tag="VE486_03255"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HZA38135.1"
                     /translation="MGGLFHKLRHPRRCYVVCAIPRCGSNLLTDGLHATRRAGVPKQF
                     FLAKFESRYGADHGLDPVSDYAGYVHGIVNAKTTSNEVFGFKLMSWYLDDFLTRLRGA
                     RVFGDAETSDLDLLQNAFPRLRFVHVIRRHKLRQALSTARALQTGLWKVQKGKTALRE
                     PQFDPELIEQCLKEAERQENLWQSFFQRIGVDPFHVEYEKLCDNYEATVRAVLDFLQI
                     SLPRNVRIGPARTVRQADEISRNWEERFLTERPASSPPAQA"
     gene            922..3519
                     /gene="gyrA"
                     /locus_tag="VE486_03260"
     CDS             922..3519
                     /gene="gyrA"
                     /locus_tag="VE486_03260"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZA38136.1"
                     /translation="MYNANEKVEPINVADEVSRSFLDYSMSVIISRALPDARDGLKPS
                     QRRILYAMHDLSLFPNRQHRKCAKICGDTSGNYHPHGEAVIYPTLVHMAQPWAMRERL
                     IDGQGNFGSVEGDPPAAMRYTEARMTHLGASLMTDMEKDTVDFVANYDETRTEPTVFP
                     AAFPNLLVNGGTGIAVGMATNIPPHNLREVVEGICAQVDDSDITLEELMKHVKGPDFP
                     TGCTIYGDAGIKQYLETGRGSMKVRGKAGVEEIKGGKEQIVITEIPYNVNRATLVERI
                     AQLVNEKTITDITAVRDESDENTRVVIELKRDANPKVVINNLYKHTALESSFAVIMLA
                     IDHGRPKLLSLKDANAAYIEHRREVVLRRTRFELRKAEERAETLEGYLIALANLDEFI
                     RIIRESANRDEARVKLLAFEFTRKQVEKVGVLIRSEARLVEARYAFSEHQANQILDLR
                     LYQLTGMERDKIEAEYKDLIKTIQDLHDILAKEKRVLTIIKKELREIADKHGSDRLTD
                     FARDEGEINMEDLIVNEGCIISITHAGFIKRTAVSAFRAQRRGGKGVIGMSTREGATE
                     EDEGDFVEHLFTATTHDYLMFFTQSGRAYVEKVYEIPEMGRAAKGRSIANILELRPDE
                     KIAATIRVQSKKSGTGATAVDQTWDENLHIVFATRSGIVKKSNLSDYANVRKGGIIAI
                     QIEEDDCLIAAKLTNGNNELVLITKQGMSLRFHEEQLRDQGRNTVGVWGIRPDKKDHV
                     VAIAIVDPNAMLLVAGENGIGKRTPFDDYRRQSRGGKGIITMKTGEKTGDVVGALTVT
                     DKDELMLITTKGQLVRTRVKEIRVVGRNTMGVKLMDLRGGEKLQAIAPVVSQAEEEAQ
                     IAEVPVASA"
     gene            complement(3965..4351)
                     /locus_tag="VE486_03265"
     CDS             complement(3965..4351)
                     /locus_tag="VE486_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HZA38137.1"
                     /translation="MKKDSVVERMARLGIRDADLEETFARSSGPGGQNVNKVATAVTL
                     RHRPSGISVTVQDSRSQAQNRKLARERLLDTIEQAQAKRRGADIAEREKARRRKSPRP
                     AALKRRILESKRRRSELKRQRARLEG"
     gene            complement(4422..4997)
                     /locus_tag="VE486_03270"
     CDS             complement(4422..4997)
                     /locus_tag="VE486_03270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HZA38138.1"
                     /translation="MPTKSERPLFIPIILGTARQGRQSENVARFVFEETKKRADVETE
                     LIDVRDLSMKLDDAGEQMKDPRFAAAIERCDGLIIVTPEYNHGYPGLLKHALDMNLKE
                     YIHKAVGICGVSAGALGGARVIEGLLPVMRELGLVTIFWDVNFGNVEKIFDEHGKLLD
                     QRYIGRLDKFLNELIWMARVLRHGREKVPPA"
     gene            complement(5072..5158)
                     /locus_tag="VE486_03275"
     tRNA            complement(5072..5158)
                     /locus_tag="VE486_03275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5123..5125),aa:Leu,seq:taa)
     gene            5188..5823
                     /locus_tag="VE486_03280"
     CDS             5188..5823
                     /locus_tag="VE486_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38139.1"
                     /translation="MKTASFAIFIVSSFVGEKYGKLLENVAMDSNEASRSAATTAKSL
                     RDALRVLNVSGWPEQPQIAGVADDAIDAAGDQCMPGLDGDQPAEPMPKDKDGPKPERT
                     TGNEERDTNPASCVAVDCPKLLSVRAGWQIRVQQAEQRKGDEDPAVATILAYTRAQIS
                     AHEERCPSHYEDSNRERDQSRVGEKGSKPTPAKHGIPKIGSGGYNDERQSS"
BASE COUNT         1407 a         1665 c         1596 g         1252 t
ORIGIN      
        1 tggacctgaa cgacgataac gccgtcgatg ccgataaaat gttcacgatt ctgatgggcg
       61 acgtcgtcga acctcgccgc caattcatcg aagacaacgc gctcaacgtg cgaaacctcg
      121 atgtgtaaaa ttcacgatgg gaggtctgtt tcacaaactg cgccatccgc gtcgctgcta
      181 tgtcgtctgc gccatcccgc gttgcggaag caatttgctg acggacggtt tgcacgccac
      241 tcgccgggca ggcgtgccga agcagttctt tctcgcaaag ttcgaatccc gctacggagc
      301 ggatcacggc ctcgatccgg tcagcgacta cgcgggatac gtgcacggca tcgtcaacgc
      361 gaaaacgacc tcgaacgaag tcttcggctt taaattgatg agctggtatc tggacgattt
      421 cctgacccgg ctgcgcggag cgcgtgtttt cggcgacgcg gaaacgagcg atctcgatct
      481 tttgcaaaat gcctttcctc gtctccgctt cgtgcatgtg atcaggcgtc acaaactgcg
      541 gcaggccttg tccacggcgc gcgcgcttca gaccggtctt tggaaagtgc agaagggaaa
      601 gactgcttta cgcgaaccgc agttcgaccc cgaattgatc gagcaatgtc ttaaagaggc
      661 ggagcgacag gaaaatctgt ggcagagctt ttttcagcgg atcggcgtcg atccgttcca
      721 tgtcgaatac gaaaaactgt gtgacaatta cgaggcgacc gtccgggcgg tcctggattt
      781 tttacaaatc tcgttgccgc gcaatgttcg cattggtcct gcccgaacgg tcaggcaagc
      841 cgatgaaatt tcacgcaact gggaagaacg ttttctcacg gagcgcccgg cctcgtctcc
      901 tcctgcgcaa gcataaccgt catgtataac gcgaacgaaa aggtagaacc aataaatgtg
      961 gccgatgaag tgtcgagatc gttcctcgat tactcgatgt cggtcattat ctcgcgtgcg
     1021 ttaccggatg cgcgtgacgg gctcaaacca tcgcaaagaa gaatcctcta cgcgatgcat
     1081 gatttgtcgc tgtttccaaa tcgacagcat cgcaaatgcg cgaaaatctg cggcgacacc
     1141 agcggtaatt atcatccgca cggcgaagcg gtgatctacc ccacgctggt ccacatggcg
     1201 caaccatggg cgatgcggga aaggctcatc gatggtcagg gcaacttcgg ctcggtcgaa
     1261 ggagatccgc cggccgccat gcgttacacc gaagcgcgca tgacccatct cggcgcctcg
     1321 ctcatgaccg acatggagaa ggacacggtc gactttgtcg ctaattacga cgagacgcgc
     1381 accgaaccga cggttttccc ggccgcgttt ccaaatctgc tcgttaatgg cggcaccggc
     1441 atcgcggttg gaatggcgac taatataccg ccccacaacc ttcgcgaagt tgtcgaaggc
     1501 atttgcgcac aggtcgacga ctccgacatc actctggaag agttgatgaa gcatgtgaag
     1561 gggccggatt tccccaccgg ttgcacgatc tacggcgacg cagggatcaa acagtatctc
     1621 gagaccggcc ggggcagcat gaaagtgcgc gggaaagctg gcgtcgagga aatcaaaggc
     1681 ggcaaggaac agatcgtgat cacggaaatt ccttataacg tgaatcgcgc cactctggtc
     1741 gagcgcatcg cccagctcgt aaacgagaag acgatcacgg acatcactgc cgtccgggat
     1801 gaatccgacg aaaacacgcg cgtcgtgatc gaattgaaac gcgacgctaa cccaaaggtt
     1861 gttatcaaca atctttacaa gcacaccgca ctggaaagct cgtttgccgt tatcatgctg
     1921 gcgatcgatc acggccggcc aaaacttctt tctctcaagg atgccaacgc tgcctacatc
     1981 gagcatcgcc gcgaagtcgt cctgcgccgg acccgctttg agctgcgcaa ggccgaggaa
     2041 cgggccgaga ccctcgaagg ctatctcatt gctctcgcta atctcgacga attcattcgc
     2101 atcatacgtg aatctgcaaa tcgcgacgaa gcgcgggtca agttgctcgc cttcgaattt
     2161 actcgaaagc aagtcgaaaa agtcggggtt cttatccgca gcgaagcacg tctggtagaa
     2221 gcccgctatg cgttcagcga acatcaggcc aatcagatcc tcgaccttcg cctctatcag
     2281 ctcaccggca tggagcgcga caagatcgaa gctgaataca aagatctcat taagaccatc
     2341 caggatctgc acgacatttt ggcgaaggag aagcgcgtcc ttaccatcat taagaaagag
     2401 cttcgcgaaa ttgccgataa gcacggatcg gatcgtttaa cggacttcgc ccgcgacgaa
     2461 ggcgagatca acatggagga tctcatcgtc aatgaaggtt gcatcatcag catcacgcac
     2521 gctggtttca tcaagcgcac cgctgtgagc gcatttcgcg cgcagcgccg cggcggcaaa
     2581 ggcgtgatcg gaatgtcaac gcgcgagggc gccaccgagg aggacgaagg tgattttgtc
     2641 gagcatctct tcaccgccac gacgcatgac tacctgatgt tttttacgca atcgggccga
     2701 gcctatgtcg aaaaagtcta cgagattcca gaaatgggac gcgcggccaa ggggcgttcc
     2761 atcgcaaata tcctggagct ccgtcccgac gaaaaaatcg ccgcgacgat ccgagttcaa
     2821 tccaagaaat cgggcaccgg ggcgaccgcc gttgatcaga cgtgggacga aaatcttcat
     2881 atcgttttcg cgacgcgctc aggcatcgtc aaaaaatcaa acctttctga ttacgctaat
     2941 gtccgcaaag gcggcatcat cgcgattcag atcgaggaag acgattgcct gatcgccgca
     3001 aaactcacaa acggtaacaa cgagctggtg ctgatcacga agcagggaat gagcctgcgt
     3061 ttccatgaag aacagttgcg cgatcaagga cgcaacacgg taggcgtgtg gggaattcgg
     3121 ccagacaaaa aggaccacgt agtcgcgatc gcaattgttg atccgaacgc gatgttgctc
     3181 gtggcgggcg agaacgggat cggcaagcga acaccatttg atgattaccg gcgtcaatct
     3241 cgcggcggca aaggcattat caccatgaaa accggtgaga aaactggcga cgttgtcggc
     3301 gctctgaccg tcaccgataa ggacgagctg atgcttatca cgaccaaagg ccagttggtg
     3361 cggacgcgcg tgaaggaaat ccgcgtcgtc ggccgaaata cgatgggcgt gaagctaatg
     3421 gacctgcgcg gtggcgagaa acttcaggcg attgcgccgg tcgtcagcca ggcggaagaa
     3481 gaagcgcaaa tcgctgaagt gcctgtcgcg tcggcgtaag ccgcctgctc aaaacgcgcg
     3541 gatgtagcca cggcgctgtg ccaccgtgcc agcaacaacg cactcagcgc ctcgacacag
     3601 cgaggcggct acaacgccaa tcgttttgcg ccggaaaaaa aaggaagact ccctgagtct
     3661 tcctttcgaa tcagcaccgc ccccagccta gtaaccgccc gtgcgatgcg tagtcgttgt
     3721 actggtcgct cccgtagcgg gctgcgttat ggcagtttca cgtgttgtcg tggtcgtagt
     3781 tgttgcggct tcttccgttg cgcaggagct caggagcatc atggcgagga ccgaaaacag
     3841 agaagtaaga atgagcgatt ttttcataac gattgtttcc tctttaatta gaattcgtca
     3901 caaatcgctt cgacagatgt attaaagcgg aaaaaagtcc aatttgtggc gatcgacact
     3961 aaatctagcc ttctaaacgg gcgcgctgcc tctttaactc gcttcgtctg cgtttcgatt
     4021 ccaaaatcct tcgctttaac gctgcaggcc gcggcgattt tcggcggcgc gctttttcgc
     4081 gctcggcgat gtcagccccg cgccgctttg cttgagcctg ctcgattgta tcaagcaagc
     4141 gctcacgcgc cagtttgcga ttttgcgcct gagatcgcga atcctgcacg gttacgctga
     4201 ttccactcgg tcgatgccgc agcgtgaccg cggtcgccac tttgtttaca ttctgaccac
     4261 ccggtccgga tgatcgtgca aaggtctctt caagatcggc atcgcgaatc ccaagccgcg
     4321 ccatgcgctc aacgacagag tcctttttca ttttggacag aatggcacag aaattactcc
     4381 gatacgattc tgttaacttt gtgaattctg tttacttccg ctcaggccgg cggaactttt
     4441 tcccggccgt ggcgaagcac tcgagccatc cagatcaact cattcagaaa tttgtccagt
     4501 cgtcctatat aacgttgatc gagcaatttt ccgtgctcat cgaaaatctt ttctacgttt
     4561 ccgaaattaa cgtcccaaaa aattgtgacc agaccgagct cgcgcatcac tggtaggagg
     4621 ccttcgatca cgcgtgcccc gccaagagct ccggcggaga caccgcaaat tccgacggct
     4681 ttgtgaatgt attctttcaa attcatgtcg agcgcgtgtt tcagtagccc gggatagcca
     4741 tgattgtatt ccggcgttac gatgatgagt ccgtcgcaac gctcgatcgc cgctgcgaac
     4801 cttggatcct tcatttgttc gcctgcgtcg tcgagtttca ttgaaaggtc gcgcacatcg
     4861 atgagctcgg tctccacatc cgcgcgtttc tttgtctcct caaaaacaaa acgcgcgaca
     4921 ttctcgctct gtctcccctg gcgagctgtt cccagaataa ttggaataaa aagcgggcgc
     4981 tccgatttgg ttggcatgtt gatctcgtct gataccgagc ttcagctttt tggcaatccg
     5041 cgatcggcaa tccgcattcc gcaatgaaat ttgccgatgg ccggactcga accggcacgg
     5101 gccttttaaa gcccaacgga ttttaagtcc gttgcgtctg ccatttcgcc acatcggcgt
     5161 tttcactctg aaacgccgtt tttacctatg aaaacagcgt cttttgcgat ctttattgta
     5221 tcatcattcg ttggcgaaaa atacgggaaa ttacttgaaa atgtggcaat ggatagcaat
     5281 gaggcttccc gttccgcagc tacgacagca aaatcactcc gggacgcgct gcgagtgctt
     5341 aatgtttcag gctggcccga gcaaccccag atagctgggg tggcggatga cgccatagat
     5401 gccgctggtg accagtgtat gcccgggctg gatggcgacc agcccgctga accgatgccc
     5461 aaggacaaag acgggccaaa gccggagcgc accaccggca acgaagagcg cgacaccaac
     5521 ccagcgagtt gtgtcgccgt cgattgtcca aaactccttt cggtccgtgc aggctggcag
     5581 atacgcgtcc aacaggccga gcagcgaaaa ggcgatgagg acccagcggt tgccacgatc
     5641 ctcgcgtaca cccgggctca gatttccgcc cacgaagaac gatgccccag ccattacgaa
     5701 gacagcaacc gcgagcgcga tcagagccgg gtgggagaaa aaggcagcaa gcccacccca
     5761 gccaagcacg gcatacctaa aataggcagc ggtggctaca acgatgagcg ccaatctagc
     5821 taagatcgac atcgacgccg cgattggttt ctctgccgat caatcatctc cacaccggct
     5881 gcacttgtag aagtgccgtt tctccggttc gcttaactcg
//
LOCUS       DASQUQ010000115         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_563944, whole genome shotgun sequence.
ACCESSION   DASQUQ010000115 DASQUQ010000000
VERSION     DASQUQ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563944"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2161)
                     /locus_tag="VE486_03285"
     CDS             complement(<1..2161)
                     /locus_tag="VE486_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA38140.1"
                     /translation="MRPLPYPDADRIMVLNESGDGQEFSVSLPNYEDWKRDNTVFEYL
                     AITRRESRNLSGIPGQEAERIPCAYVSEDFFKVIGLSPKLGRTFTEQEAKPGGPPAVV
                     ISDRLWQRLFARGSGVLGQTIKLHNQGFTIIGVMPPEMTSPQDTDVWFPFARRVTPIW
                     RDRMIHPMLYAWGRLKPSVTVDQARTQMKGIAARLEKEYPATNARASVTVTPLLENLI
                     GKYRTNLTLLLGAVGLVLLIACANIANLFAARGAARAREFAIRAAVGASRGEIIRQLL
                     VESFLIALLGGALGFFIAVWVRDALIALAPQGVSRFQDISFDLPVLCFSFAIASLTTI
                     FFGLWPAWQTSRANMQLALQAGSPSGGDAPAAKRTREWLIISEIALTLTLLVAAGLIL
                     KSFSRMQALKLGYEPHGLITAKMELPFRVYSNREKVMAFTNALLEKVRALPGVQSVGI
                     GSNSPLMGGWQIGFYREGISTPTPSEMPTADLEVVAGDYFTAFKVPLLRGRTFNAADT
                     ANSPRVIVIDQSLAEQIFPGEDPIGKRLVVDVGNDGEGYALAEIIGVVARMRFHAIDE
                     TVQFPVIFCSMAQAYRAGFGLFVRAGAGAASLEKPIRDAVSSIDPAQPVFDVRLMQDR
                     VAETWGTQRLLSFLFSIFAGLALALASIGLYGVLTYTTLKQLREIGIRMALGARPSQI
                     RALVLSKGMQLLLIGSCIGFVAILALSRVLQSVLFEVQ"
BASE COUNT          545 a          627 c          615 g          422 t
ORIGIN      
        1 cttgaacttc gaaaagaaca ctttgcaaca cccgggagag cgccaggatc gcaacgaacc
       61 cgatgcacga gccgatcaac agcaactgca ttcctttcga gaggacaagc gcgcgaatct
      121 gagacggacg agcgccgagc gccatgcgaa tgccaatttc gcgaagctgc ttgagtgtgg
      181 tgtaagtcag aactccgtac aacccgatgc tggcaagcgc gagagccaga ccggcgaaaa
      241 tggaaaagag aaagctgaga agacgttgcg tgccccacgt ttccgcgacg cgatcttgca
      301 tgagacgaac atcgaacacg ggctgagccg gatcgatgga acttacagca tcgcgaatcg
      361 gtttttcgag tgaagctgcg cctgctccgg cgcggacaaa taagccgaat cccgcgcgat
      421 acgcttgcgc cattgaacag aaaatcaccg ggaactggac cgtttcatca atggcgtgaa
      481 accgcatccg cgcgactact ccgatgattt cggcaagggc atagccttcg ccgtcgttac
      541 cgacatcaac cacgaggcgt ttgccaatcg gatcttcgcc gggaaaaatt tgctccgcca
      601 agctttgatc aatcacgatg acgcgcggcg aattcgctgt gtcggccgca ttgaaagtgc
      661 ggccacgcag cagcggcact ttgaacgccg tgaaatagtc gccggcgaca acttcaagat
      721 cggcagtggg catctcactt ggtgtgggag ttgaaatgcc ttcccgatag aaaccgattt
      781 gccaaccacc catgagcggc gagttcgaac cgatgccgac actttggact cccggcagcg
      841 cacgcacttt ttcgagcaaa gcattcgtaa acgccattac cttttcgcga ttcgaataaa
      901 cccggaaggg aagctccatc ttggcggtaa tcagaccgtg cggttcgtaa ccgagtttga
      961 gagcctgcat gcgcgagaaa ctttttagaa tgagcccagc cgcgactagt aacgtgagcg
     1021 tgagcgcgat ctcgctaatg atgagccatt cgcgggtgcg tttggcggca ggcgcgtcgc
     1081 cgccgctggg cgaaccagct tgaagagcaa gttgcatgtt tgcgcgtgat gtttgccacg
     1141 ccggccacaa tccgaagaaa attgtggtga gcgacgcgat ggcaaaactg aaacagagaa
     1201 ccggcaaatc gaacgagata tcttgaaatc gggaaacgcc ttgtggcgct aacgcaatca
     1261 acgcatcgcg aacccaaact gcgatgaaaa agccaagcgc accgccaagc aacgcgatca
     1321 gaaaactttc gaccaaaagt tggcgaatga tctctccgcg actcgcacca acggccgcgc
     1381 gaatggcaaa ctcgcgcgct cgcgcggcgc cacgagcggc gaaaagattc gcaatattcg
     1441 cacatgcgat gagcaggacc aatccgaccg cgccaagaag caaggtgaga ttagtccgat
     1501 atttgccgat cagattttcg agcaacggtg tgactgtcac gctggcccgc gcgttcgtgg
     1561 ccggatattc tttttccagc cgcgcagcga tgcctttcat ctgagtgcgc gcctgatcga
     1621 cagttacgct gggcttcagt cgcccccatg catacagcat cggatgaatc atgcggtcgc
     1681 gccaaatggg agtcacgcgg cgagcgaatg gaaaccagac gtccgtgtcc tgcggcgacg
     1741 tcatctccgg cggcatcacg ccgatgattg tgaaaccctg attgtgaagt tttatcgtct
     1801 gacccagaac acccgagccg cgcgcgaaaa ggcgctgcca aagtcgatcg ctgataacga
     1861 cggcaggcgg cccaccaggt tttgcttcct gttcagtgaa ggtccgtccc aatttgggcg
     1921 agagcccaat cactttgaaa aaatcttccg agacataagc gcaaggaatt cgctcggcct
     1981 cctgtcccgg gatcccgctc aggtttcgcg attcgcgccg cgtgatggcg aggtattcaa
     2041 agacagtgtt gtcgcgtttc caatcctcat aattcggcaa cgaaaccgaa aactcctgac
     2101 cgtcgcccga ttcgttcaat accatgatcc ggtctgcatc cgggtatggc aggggccgca
     2161 acaggacagc gttaacaatg ctaaagatgg ccgtgttcgc tccgatgcc
//
LOCUS       DASQUQ010000116         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_489847, whole genome shotgun sequence.
ACCESSION   DASQUQ010000116 DASQUQ010000000
VERSION     DASQUQ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489847"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /gene="epsC"
                     /locus_tag="VE486_03290"
     CDS             complement(<1..812)
                     /gene="epsC"
                     /locus_tag="VE486_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HZA38141.1"
                     /translation="MDSTNRDAVEELLQTYGETGGINYLAAAATLPSRLAIEAACAEL
                     MSLMFPGFRSEALVSSGDLADTTRTRIRNLHARLKTEICRSLGKIPPNEATEAKAEEF
                     LSVFLKELPSVRRVLWTDIDAAYEGDPAAKSYEEIILAYPALEAIAIYRLAHLLYDKV
                     PLIPRIMTEWAHSRTGIDIHPGAKIGSHFFIDHGTGVVIGETTEIGSHVKLYHGVTLG
                     ARSFQKDEHGKIKKGGKRHPKVEDDVTIYPNSTVLGGETVIGARSTIGGNVFL"
     gene            complement(1190..1888)
                     /locus_tag="VE486_03295"
     CDS             complement(1190..1888)
                     /locus_tag="VE486_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZA38142.1"
                     /translation="MKVARSTNKNPRCDPPDAKRIVIVDDHPLFRKGLEQLIHSDGAF
                     AVCGEAGNAAEAMEVIRKLNPDLAIVDLSLPGANGIELIKNIRAEFPKQPILVLSMHD
                     ESLYAVRALRAGAQGYVMKHEAMANVVQAIHEVFSGRPYLSRIMAAQVITKFAHGPSE
                     GEADPVDKLSDRELEILELIGKGKDVREIAKLLHLSPKTVETHRAHIKDKLDLKNARE
                     VARFAVQWLGARSG"
     gene            complement(2032..2913)
                     /locus_tag="VE486_03300"
     CDS             complement(2032..2913)
                     /locus_tag="VE486_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZA38143.1"
                     /translation="MNRTKIGRGSTRKLVRGRIDSLQKSKELLEKVVRERTRALHLSN
                     AELKNEIERRKGLEEEILAISDREQQRLGQELHDGLCQHLTAVAFMARSVALRLKNHR
                     VIEVGDIEKIAELVNNAAADMRNLSRALHRIDVDAAGLVAALQDLVDRKIWKVPCRLE
                     VKPSFHTEDDAAAAQLYRIAREAVINANKHAQAREIVIKLEQSPKGMVLRVTDDGVGF
                     SDEPKLKPGLGYHIMKYRAQLIGGRLEIGSPRRGGTCVSCYLPNNTRQPLKSHKKENA
                     EGRRLSSKIAKALATPI"
     gene            complement(3088..3234)
                     /locus_tag="VE486_03305"
     CDS             complement(3088..3234)
                     /locus_tag="VE486_03305"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HZA38144.1"
                     /translation="MRFILLLLLLLLVIAALPAWPYSAGWGYYPSGGLGLVLLIILLV
                     ILFR"
     gene            3370..3555
                     /locus_tag="VE486_03310"
     CDS             3370..3555
                     /locus_tag="VE486_03310"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZA38145.1"
                     /translation="MTNLQFKGSWNEIKGKLKQKYGQLTDDDLAFAEGKEDELLGRLQ
                     QKLGKTKEDLRKEIESL"
     gene            3633..3824
                     /locus_tag="VE486_03315"
     CDS             3633..3824
                     /locus_tag="VE486_03315"
                     /inference="COORDINATES: protein motif:HMM:NF022890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HZA38146.1"
                     /translation="MPAKSKKQQMAAGAALAAKRGERKKSSLKGASKGMYKSMSKAEL
                     HRMAKTKRKGKPTKKAKKR"
BASE COUNT          933 a         1089 c         1040 g          920 t
ORIGIN      
        1 agaaaaacat ttccgccgat ggtgctgcgc gcgccgatca ccgtttcgcc accgagcacg
       61 gtggaattcg ggtaaatggt gacgtcatcc tcaactttcg ggtgtcgttt gccgcccttt
      121 ttgattttgc cgtgctcgtc cttctggaaa ctgcgtgcac cgagagtgac gccatgataa
      181 agcttcacat gcgaaccgat ctcggttgtc tcgccgatta cgaccccggt cccgtgatcg
      241 atgaaaaaat gtgaaccgat ctttgcgccg ggatgaatat cgatcccggt gcggctgtga
      301 gcccattcgg tcatgatgcg tgggatcaag ggcaccttgt cgtatagaag atgcgccagt
      361 cgataaatcg caatcgcttc cagggcggga taagcgagaa tgatttcctc ataacttttc
      421 gcagccggat cgccctcgta agcggcatcg atgtccgtcc aaaggacgcg acgtactgac
      481 ggcaactcct ttaaaaacac agacaagaat tcttccgctt ttgcttcggt cgcttcgttg
      541 ggcgggattt tgccgaggct ccggcaaatt tctgttttca atcgagcgtg caaatttctt
      601 atacgggttc gagtggtgtc agccaaatcg ccagagctga ccagtgcctc gctccgaaag
      661 cccggaaaca tcaggctcat caactcagcg catgctgctt cgatggcgag acgcgagggc
      721 agcgtggcgg cggcggcgag ataatttatg ccgcctgttt cgccataagt ctgtagcagt
      781 tcctcaaccg catcccggtt cgtcgaatcc attgcaggaa atatgcgctc ggttccttct
      841 caacaaaaga gaaagtgtgg acgaaaggcc gcgtgggttt gttgaagcgt taaagcgctc
      901 aaagcaatgt gaagcgttcg ttacggattg ctgttcgacg gcacggtgaa tagcggcgca
      961 gagcgccgca ctccaaaacg tggccgtaat cgccagcgca cgtttttcgc ttgcgtcttg
     1021 gattgcggca gcgctctgcc gctttgaaac gcggtgtcaa acgctcaacc gccaacatcg
     1081 aacctgcaga attcgtagct tcgacgcttc ctgctctttc ctgaaaaggc ctcccattcc
     1141 ggcaaagaag agacccgcca accccacagt caagatggca gtgctacgac tagccgctgc
     1201 gcgcgcctag ccactgaacg gcgaaacgcg cgacttctcg cgcgtttttc aaatcaagtt
     1261 tgtctttgat gtgcgcccgg tgggtctcca ctgttttggg gctgagatgc aaaagcttcg
     1321 caatctcgcg cacgtccttg cccttgccga tcaattccag aatttcaagc tcacgatcgc
     1381 taagtttatc cacggggtcg gcctcgccct cagacgggcc atgtgcgaat tttgtgatga
     1441 cttgtgccgc cattatccgg ctgagatagg gtcgcccgct gaagacttcg tgaattgcct
     1501 ggacgacatt cgccatcgcc tcgtgcttca taacgtaacc ctgcgctccg gcacgaagcg
     1561 cgcgcacggc gtagagcgat tcatcgtgca tcgagagcac cagaattggc tgcttgggaa
     1621 attcagcgcg aatgttttta atcagctcaa tgccgttggc gcccggcaaa ctaagatcga
     1681 cgatcgccag atcgggattc agtttgcgaa tcacctccat cgcttccgcg gcgttgcctg
     1741 cttcaccgca aaccgcgaag gcgccatcgg aatgaatcaa ttgctcgagg cctttgcgaa
     1801 acagcggatg atcatcgacg atcacgattc gcttcgcgtc cggcggatcg catcgcgggt
     1861 tcttgtttgt tgaccgcgcg actttcattt aaggtgtgcc cgtcccgccg cctttcccaa
     1921 tttgccgcaa cggattgtat cccgctgcga ttgctgcgcg gcgagtcatc aatttccctt
     1981 caatcctcta ttaacaattc gcctcccggc aggctgtcag atggagaaaa tttagattgg
     2041 tgttgccagc gccttcgcga ttttcgagga gagccgtctt ccttcggcgt tttctttctt
     2101 gtgtgatttg agtggttgac gcgtgttgtt cggcaaataa caggagacac acgtgccgcc
     2161 ccgtcttgga gagccaattt ccaaacggcc gccgatcaat tgagcccggt acttcatgat
     2221 atgataaccg agccctggct tcaatttcgg ctcatcggaa aatccgacgc cgtcatcggt
     2281 cacacgcaaa accattccct ttggcgattg ctccagtttg atcacaattt cccgcgcctg
     2341 ggcgtgctta ttggcgttga tgactgcttc gcgtgcgatg cgatagagct gcgctgcggc
     2401 cgcgtcatcc tcagtatgaa acgatggctt gacttccagc cggcagggta ccttccagat
     2461 tttccggtca accaagtctt gcaatgcagc gaccaagccg gctgcgtcga catcgatccg
     2521 gtgcaaggcg cgggaaagat tacgcatatc ggccgcagcg ttgttcacta gctcggcgat
     2581 tttttcgata tcacctactt cgatgacgcg atggttcttc aaccgcaacg cgaccgagcg
     2641 cgccatgaat gccactgcag tgagatgctg acaaagcccg tcatgcaact cctgtcccag
     2701 ccgctgttgc tcccggtcgc tgattgccag gatttcttct tccaagccct tccgccgctc
     2761 gatctcattt ttcaattcgg cgtttgagag atgtaatgcc cttgtgcgct ctcggaccac
     2821 tttctccagc aattccttcg atttttgcag cgaatcaatg cgaccccgga cgagtttccg
     2881 cgtgctgcca cggccaattt tcgttctgtt catgtcctgg aacggaatct gaatcccaac
     2941 accgatctta atcgtaaagc gacagtgctt aaaggcaaaa gcattggatc gcgctggtcc
     3001 tctggggatg gtgaaacccc gcctcgagtt tggtttgtaa aaactacctc acgcgcactg
     3061 agaccgatgg cacgcgtatc gcaatctcta gcgaaacaaa atcaccagca atataatgag
     3121 cagaacgaga cccaaaccgc cgctgggata atatccccaa ccggcgctat atggccatgc
     3181 cggtaaagcg gctattacca gcagaagcag aaggaggagc aggatgaatc gcatggttcc
     3241 tttctttgtg tgggtatttg aattggatga ttctacaaag gaatttcgcg ccagacgagt
     3301 cgcatgcgcg tatcactttc atcgcgaaag gcaactgacc ccggtgtcgc gtgcgtattc
     3361 tttattacta tgacaaacct acaattcaaa ggcagctgga acgaaataaa aggcaaactc
     3421 aaacagaaat acggacagtt aaccgatgac gatctcgctt tcgctgaggg aaaggaggac
     3481 gagttgcttg gtcgccttca acaaaagctc ggcaaaacca aggaagatct ccgaaaagaa
     3541 atcgagagcc tgtaacgaga cggtcgaaaa tgagatgcgt ctcaccgcgt ccccttattc
     3601 gaactaacca aaacaaaagg aggaaaggtt ttatgccggc aaaatcaaag aaacagcaaa
     3661 tggctgcggg cgcagcgttg gccgccaaga gaggcgaacg caagaagtcc tctctcaagg
     3721 gcgcatccaa gggcatgtat aaatcaatga gcaaagctga actgcacaga atggcgaaga
     3781 cgaaacggaa aggcaagccg acgaagaaag cgaagaagcg ctgagtcagc cattcgcggg
     3841 gaaaagcgat cgaatttcaa tcagctacaa aaagctgatt gaatctgggc tgcgcgatta
     3901 cgagcacgat acactgagtt gcgacttttg gtcgcattcg tcgccggttt cagaaaagct
     3961 aaatattgcg catcgaaagc gc
//
LOCUS       DASQUQ010000117         4902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_491172, whole genome shotgun sequence.
ACCESSION   DASQUQ010000117 DASQUQ010000000
VERSION     DASQUQ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491172"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VE486_03320"
     CDS             complement(<1..651)
                     /locus_tag="VE486_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZA38147.1"
                     /translation="MHPSHLEDFYSFLRFPSVSTDTQFAHHVRDCAQWVAQKLKAVGL
                     ESEVVATKGHPIVWARNQHQPGRRTILIYGHYDVQPPDPLELWDSPPFEPVLKDGYVF
                     ARGATDNKGQILAHILGIQETIEQDGDLPVNLHLVIEGEEEIGSVHLGSFLEKNRDAL
                     KSHVAVISDTGMIAPRTPTLSYGLRGVTALEIKVTGPKMDLHSGIFGGAVANPITAL"
     gene            872..2188
                     /locus_tag="VE486_03325"
     CDS             872..2188
                     /locus_tag="VE486_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZA38148.1"
                     /translation="MHAPPAPKEEAGLIRAIGVPGLTANIVNSTVGAGIFALPALVAA
                     QLGAASPVAYIICALAMCLFVTSFAMAGSRVSLTGGLYAYVETAFGRYVGFLAGVLYF
                     LTAILAISGIVGLIASSVGNLIPSLATQLGRFVIILSTLLFLAAINIRGVKIGARAVE
                     TVTVIKLAPLIIFVIAGIFFLRPEALAWPGWPQTDALGRSVLLLLFAFVGVEVALVPS
                     GEIKQPARTVPRAIFTALGITTLLYIMIQLVALGVLGSELGEYGDTGLAEAAARFLGN
                     AGRTLMLAGLAISAFGWTTSDILSSPRIIFAFGRDGFLPKWFAHVHPRFHTPDVAIIT
                     YAATAFALSFSSTFQKLAVLSNMAVLLLYILCCLAALELNRRDTRSDPATAGFKFPGQ
                     SIVPIVAIVFIIWVLAHATKEEFVITAACLAAATLLFFIRKFAGAR"
     gene            2391..>4902
                     /gene="smc"
                     /locus_tag="VE486_03330"
     CDS             2391..>4902
                     /gene="smc"
                     /locus_tag="VE486_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZA38149.1"
                     /translation="MHLQSLELLGFKSFADKTTFNFHEGITAIVGPNGCGKSNVLDAV
                     RWVLGEQSAKSLRGDEMADVIFNGSDMRKPVGFAEVSVTFGNCAKELQVDWHDVRVTR
                     RVYRDGNSEYFLNKTPCRLRDIQNLFADTGVARAAYSMMEQGKIDMVLSSRPEDRRAV
                     FEEAAGITKYKTQKRDAMRKLEATEANLLRIGDIIKEVKRQIGSLQRQAGKARRYQAL
                     HADLRVLDTHHSHKQLDSLKAELVRCHAEIARLGESEQACRAKIETSENQIAEQRRAL
                     DEIDARIADGRSELQRLQSQIGTHHNQIQFNRQRAEELTELIERSRRDVAAAEAKRAQ
                     QDKELEAANALVEKTSQLLQAKEAELAKLTDLIAKFRSERDACDSKLEGLRSSASESE
                     ERMAELEDEISGLAIRRDATEETRRDLDAAISKARAAQDKVRKELATARAAAETGQKN
                     LNLLKTKSQADEKTLRQQQERLAKTEKSLTEIERSLAEKESRLEILRQLNEEGEGLAQ
                     GSQSVLKGLDNPSRIQPGLAGALVSNLDVDPEFIAAIEAAMGRNLQAIVLKNSQLAPE
                     IIAKLKEKKIGQAALVLPGLSNSSTETHAALPQQALAWAREKVKAPDSLAPLVARLLH
                     NVAIFRDLDAALSFKKQGSDFAAATLAGEFISAEGIVFGGSRDGSADSLLERKARISG
                     LNAEYAEIGRQRDALLQKRDDANAILEKTAGECEEARSQFASADRDQSACENRILFLE
                     QESQRSEQNLEQLRSEQTVLAQQIQTADEQIAKLEEELGSDRAASEAQQNEQLELQGA
                     REKAAESKDEATEQLNQLRLAFATERERYENLVAQRQPM"
BASE COUNT         1108 a         1367 c         1393 g         1034 t
ORIGIN      
        1 gagtgcggtg attggatttg caactgcgcc gccaaatatg ccggagtgca aatccatctt
       61 cggcccagtg actttgattt cgagggccgt cacgccgcgc agtccgtagc ttagggttgg
      121 cgttcggggc gcgatcattc cagtgtcaga aatcaccgcc acatggcttt tcagcgcatc
      181 acgatttttc tccaaaaaac tcccgagatg tacgctgccg atttcttctt cgccttcgat
      241 tacgaggtgc aggttcaccg gcagatcgcc atcttgttcg atcgtctctt gaataccgag
      301 aatgtgcgca agaatttgtc ccttgttgtc ggtagcgccg cgcgcaaaga cgtagccgtc
      361 cttcaaaaca ggctcgaacg gcggcgaatc ccaaagctca agagggtccg gcggttgcac
      421 atcataatgt ccgtaaatca aaatggtccg acgcccgggc tgatgctggt tgcgggccca
      481 aacaatcggg tggcccttcg tggcaactac ttcggactcg agtccgacgg ccttcagctt
      541 ttgcgcgacc cattgcgcgc aatcgcggac gtgatgcgca aactgagtgt cggttgatac
      601 gctcggaaac cgcaggaacg aataaaaatc ttcgagatga ctcgggtgca tgggacgaac
      661 cttaaccccg atttagggaa aacgtccaac tccgatgaat cgcgagcggg cgtcgaacgt
      721 tcagcaccct ccgagtgcaa cgaatttgta gggcgggcgc gtcgcctgcc cggcgcagac
      781 tcgccaagcg gaccgcttgc cctgcaactg cgctctcgcg atttggaagt tcggcgttga
      841 acgtcgagcg tttcttgacc aatctgcgcg catgcacgcg ccaccggctc cgaaagaaga
      901 ggccgggctg attcgcgcga tcggtgttcc cgggctcacc gctaacatcg tcaactcgac
      961 ggtcggcgct ggcatttttg ctttgccggc gctggtcgcg gctcagctcg gcgcagcatc
     1021 tccggtcgca tatatcattt gcgctcttgc catgtgtttg ttcgtgactt cgttcgccat
     1081 ggcgggcagc cgcgtctcgc ttacaggtgg tctctacgcc tatgtcgaaa ctgcctttgg
     1141 acgttacgtc ggttttctcg ctggcgtgct ctatttcctc accgccatac tcgcgatttc
     1201 cgggatcgtc ggtctcattg ccagttcagt gggaaacttg atcccgtcgc tcgcgacgca
     1261 gctcggtcgc ttcgtaatca ttttgtcaac cctgctgttt cttgctgcaa tcaatattcg
     1321 cggcgtaaag atcggcgcgc gtgcggtcga aacggtcacc gtgattaaac tagcgccgtt
     1381 aatcattttc gttatcgccg ggattttttt tctccggccg gaagcgctgg cgtggccagg
     1441 ctggccgcaa actgacgcgc tgggcagaag cgttctccta ttgctcttcg cgttcgtagg
     1501 cgtggaggtc gcgctggtgc cgagcggcga aatcaaacag cccgcacgaa cagtcccgcg
     1561 cgccattttc accgctctcg ggatcacgac tttgctttac atcatgatcc agcttgtcgc
     1621 gctgggtgtt ctcggcagcg aactggggga atacggagac accgggttgg ccgaggccgc
     1681 tgcgcgattt ttgggaaacg ccggaagaac gctgatgctc gccgggctgg ccatctcggc
     1741 ctttggctgg acgacgagcg atattctgag ttcgccccgg atcattttcg ccttcggacg
     1801 agatggtttt cttccaaagt ggtttgcgca cgtgcatccg cggttccaca cacctgacgt
     1861 cgcaatcatc acctacgccg caaccgcgtt tgctctttcc ttcagctcga cctttcaaaa
     1921 actcgcggtt ctttctaaca tggccgtgct gttgctctat attctgtgct gtctcgccgc
     1981 gctggaattg aatcgacgcg acacccgcag cgatcccgcc actgcgggat ttaaatttcc
     2041 tggccagagc attgtcccga ttgtcgcgat tgttttcatt atctgggtcc ttgcccacgc
     2101 gacgaaggaa gaattcgtaa tcacagccgc ttgtctggca gcggcgacat tacttttctt
     2161 catccgcaaa ttcgcgggag cacgatgaat agccagattc gaagctgaaa gctttggatt
     2221 ctgcaggtac gtttgaggtc gggaattcga ggtttcgagc cgcctggccg ggacacaaga
     2281 tcatgtgccg gtccacagag ctgaccactt caaatggtgc aaatttagct tgtaacccca
     2341 gcaacctctc tatattgaac ctccgagggc cccgaggttt cggcctgcgc atgcatttac
     2401 aatcgctcga gctactcggt tttaaatcgt ttgctgataa gacgaccttc aatttccacg
     2461 aaggtatcac ggccatagtc gggccgaacg gctgcggaaa atccaacgtg cttgatgccg
     2521 tccgttgggt gctgggggaa caatctgcca aatcgctccg aggcgatgag atggccgacg
     2581 tgatttttaa cggcagtgac atgcgcaagc cggtcggctt cgcagaggtc tctgtcacgt
     2641 tcggcaactg cgccaaagag ctgcaagtcg attggcatga cgtgcgcgta acgcgccgtg
     2701 tgtatcggga cggtaactcc gaatacttcc tcaacaaaac cccctgccgt cttcgcgaca
     2761 ttcaaaatct tttcgctgat accggcgtcg cccgggccgc ttattcgatg atggaacagg
     2821 gcaagatcga catggtcttg agttcgcgac cggaagatcg ccgcgccgtt tttgaagaag
     2881 ctgccgggat cacaaaatac aaaacgcaga aacgcgatgc gatgcgcaaa ttggaagcga
     2941 ccgaggcaaa tctgttgcgc atcggcgaca tcattaaaga agtgaaacgc cagatcggct
     3001 ctttgcagcg gcaggcgggg aaggcgcggc gctaccaggc attgcacgca gatctgcgcg
     3061 tgctcgacac gcatcattcc cacaaacagc tcgattcgct gaaggccgaa ctggtccgtt
     3121 gccacgccga gatcgcgcga ctcggcgaat ccgaacaggc gtgccgggcg aagatcgaga
     3181 cgagcgaaaa ccagatcgcc gagcagcgtc gcgcgctgga cgagattgat gcccggattg
     3241 cggatggtcg cagtgaattg cagcgcctgc aaagccagat cggcacgcat cacaatcaga
     3301 tccaattcaa tcggcaacgc gcagaggaat taacggagtt aatcgagcgt tccaggagag
     3361 acgtagctgc ggccgaggcg aagcgcgctc agcaagacaa agaattggag gcagccaatg
     3421 cgctggttga aaagaccagc cagcttcttc aagccaaaga agccgaactg gcaaagctca
     3481 ctgacttgat cgcgaaattt cgctctgagc gggatgcgtg cgactcgaaa cttgaagggc
     3541 tgcgatcgtc agcgtcagaa agtgaggagc gaatggccga actcgaagac gagatctccg
     3601 gacttgcgat tcgccgcgat gccacagagg aaactcggcg cgatctcgat gctgcgattt
     3661 ccaaggcgcg cgccgctcaa gacaaggtcc gcaaggagct cgctaccgcg cgcgcggcgg
     3721 cagagaccgg gcaaaaaaat ctaaatcttt tgaagacgaa atcgcaggcc gacgagaaga
     3781 cattgcgcca acaacaagag cgtctcgcca aaactgaaaa aagcctgacc gaaatcgaac
     3841 gctctttggc ggaaaaagaa tcacgcctgg aaattctgcg ccagcttaat gaagaaggcg
     3901 aaggtctcgc tcaaggttcg caatctgtgc tcaaaggcct tgataatccg tcacggatcc
     3961 aacccggtct ggcgggagct ttggtttcga acctggatgt cgatccagaa tttatcgcgg
     4021 caatcgaagc tgccatgggt cgaaatctac aagcgattgt tctgaaaaac tcgcagctgg
     4081 caccggaaat catcgccaaa ttaaaggaaa agaaaatcgg gcaggcggcg ctagttctcc
     4141 cgggcttgtc gaattcttcg actgaaactc acgcagccct tccgcagcaa gccctcgcct
     4201 gggcgcgtga aaaggtgaaa gcgccggatt ctcttgcgcc gctcgtggcg cggttactgc
     4261 ataatgtcgc aatttttcgc gatctggacg cggctctctc tttcaagaaa caaggcagcg
     4321 atttcgccgc ggcgacgctc gcgggagaat tcatttcagc ggaaggaatc gtttttggcg
     4381 gcagccggga cgggtctgcc gattctttgc tcgaacgcaa agcgcggata tcgggtctga
     4441 acgcagaata cgcagagatc ggcaggcagc gcgatgcatt gctgcaaaaa cgcgatgacg
     4501 caaatgcaat attagaaaaa acggcaggcg agtgcgaaga agcccgcagt caatttgcgt
     4561 ctgccgaccg ggatcaatcc gcctgcgaga atcgaattct cttcctggaa caggaatcgc
     4621 agagaagtga acagaatctc gagcaacttc gctcggagca gacagtcctc gcgcagcaaa
     4681 tccaaaccgc agacgagcaa attgcaaaat tagaggaaga actgggctca gatcgcgccg
     4741 cttcggaggc gcagcaaaat gagcagctcg aacttcaggg tgcacgcgag aaagcggcgg
     4801 agagtaaaga cgaggcgacg gaacagctca atcaactgcg tcttgcgttc gctaccgagc
     4861 gggagcgcta cgagaacctc gttgcacagc gccagccgat ga
//
LOCUS       DASQUQ010000118         3810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_668803, whole genome shotgun sequence.
ACCESSION   DASQUQ010000118 DASQUQ010000000
VERSION     DASQUQ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668803"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VE486_03335"
     CDS             <1..453
                     /locus_tag="VE486_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZA38150.1"
                     /translation="NGKFTQFSGTIDLDREHPERSSVSARIQVRSIDTRIKKRDDHLR
                     SEEFFNVAKYPEITFKSRSVKQTGPQSGEIIGDLTMHGVTKPVTLHVKLITPLTGEGS
                     LQRTRWEVTTAPLKRRDFGLMFGSTTEAVSGISQDVAIDIEIEAKRAE"
     gene            609..1331
                     /gene="pxpB"
                     /locus_tag="VE486_03340"
     CDS             609..1331
                     /gene="pxpB"
                     /locus_tag="VE486_03340"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HZA38151.1"
                     /translation="MEIVPLGDSALIVRVRDRFEDAPEETLDEVLRTFDRVRSAAIPG
                     VTEVTPAYTSVAVFFDPLQVAKASGQRDRAFDWLDEQVRRVVAAGADRGGPRKARRIT
                     RSVEIPACYDPEFAPDLDRVARHAEISPNEVVYLHSAAEYRVSCIGFTPGFPFLSGMP
                     KKLATPRLDVPRKEIPVGSVGIGGAQTGIYPMPSPGGWNVIGRTPLQLFDPEKDPPAL
                     LQAGDCVRFRTITREEFEAMRP"
     gene            1438..2553
                     /locus_tag="VE486_03345"
     CDS             1438..2553
                     /locus_tag="VE486_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HZA38152.1"
                     /translation="MSLKIVRSGFLTTVQDLGRVGFRQFGVSSGGALDLHAMRIANLL
                     VGNEESAAGLEITQGNVRLRFDDERIISWCGGDYEFRIASYAIPAGHSARISAGDELS
                     ICGPQRGCRAWIAISAGIGVPSVLGSRSTDLRGNFGGLHGRAVRDGDSLPLGKNSKRT
                     KIWMEDLRAERIARWSAPEPWSQTATAAPMLRVIRGADWNRFNPPSPGYGVAGDVSIQ
                     RFTSEAFTVSPDSDRMGVRLAGPELHRTNDTELISEAVAPGTIQVPPDGKPILLLGDC
                     QTIGGYPKIAHVITVDLSIAAQLRAGDQVRFSEVSLADAHGLLLERERDFERFRIGLS
                     LLASRDSSCSHGPAGRSGASNGRAGGRRATGPWLQGT"
     gene            2553..3308
                     /locus_tag="VE486_03350"
     CDS             2553..3308
                     /locus_tag="VE486_03350"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HZA38153.1"
                     /translation="MKLSVDINADLGEGAGHDAELFELISSASIATGFHAGDADTMHA
                     AVLEAKAHGVAVGAHPSFFDRENFGRKELKVSNQEVFDAVAYQLGIFQAIAVAAGLVP
                     NHVKPHGALYNMAVRDGKLAEAIARAIKSVDSKLILFAPDHTDLSRAGEALGLQIARE
                     VFADRNYLADGWLVPRTRADALLTDPAEAAKRVLRMLREGKVRSVEGDDVEVRVETIC
                     VHGDTPGAVEFARELRSQLESQGVRISAPHREL"
     gene            3305..>3810
                     /locus_tag="VE486_03355"
     CDS             3305..>3810
                     /locus_tag="VE486_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZA38154.1"
                     /translation="MSNDIELQKQSGLVRALGPLDATMIVMGSMIGSGIFITSAESAR
                     LIGAPGWLLMAWAVAGLMTITGALCCSELATMMPRAGGVYVFLREAYGPSVGFLFGWT
                     LFLVIQTGTIAAVAIAFAKFLGVFVPAVGPENYLVRPIPLGAHYAISLSTEQLIAIAL
                     IALLTWTNT"
BASE COUNT          773 a         1032 c         1143 g          862 t
ORIGIN      
        1 aacggcaagt tcactcaatt ctccggcacc atcgacctgg accgtgagca tccggagcgt
       61 tcatcagtct cggctcggat tcaggtgcgc agcatcgaca cgcgaattaa gaagcgcgac
      121 gatcacctgc gcagtgaaga attttttaac gtcgcaaaat atcccgagat cacattcaag
      181 agccgcagcg tgaaacagac tggcccgcaa agtggcgaga ttatcggcga tcttaccatg
      241 cacggggtga cgaaaccagt gaccctacac gtgaagctca tcactccttt aaccggcgaa
      301 ggttcactgc agcgaacccg ctgggaggtg accactgcgc ccctcaagcg tcgcgatttc
      361 ggattaatgt ttggcagcac aacggaagcc gtctcaggca tcagccagga tgtcgcgatc
      421 gatattgaga tcgaagcgaa gcgagcagag taagcgatcg tcgctgggcg ctctttatat
      481 tgtcattcag agcccttctg ctttgctcag ggcaggctcc gcgaagaatc tctgattatt
      541 ttcatttcaa ttacggcgag agaacaaaca gagatgtttc gcttcgctca acatgacagg
      601 ataacctgat ggaaattgtc cccctgggag attccgccct catcgtgcgc gtgcgcgacc
      661 gatttgaaga tgcgcccgag gaaacattgg acgaggtcct gcgcacattt gaccgggtcc
      721 gaagcgctgc tattccgggt gtcaccgaag tcacgccggc ttataccagt gtcgcagttt
      781 tcttcgaccc gctgcaggtt gcgaaggcaa gcggccaacg agatcgcgca ttcgattggt
      841 tagacgagca agttcgccgc gttgtagccg cgggcgctga ccgcggcgga cctcgcaaag
      901 ctagaagaat tacacgctca gtcgaaatcc cagcctgcta tgatccggag tttgcccccg
      961 atcttgatcg cgttgctcgc catgcagaaa tatcgccaaa cgaagtcgtc tatcttcaca
     1021 gcgcggctga atatcgggtg agctgtattg gatttacgcc gggttttccg tttctctcag
     1081 gtatgccgaa gaaactggcc acgccgcgcc tggacgtacc gcgaaaggaa attccggtcg
     1141 gctcagtcgg aataggcggc gcgcaaaccg gaatttatcc gatgccctcg cccggcgggt
     1201 ggaacgtaat cggccgcaca ccattgcaac tgttcgatcc ggaaaaggat cctccagcgc
     1261 ttctgcaggc cggcgattgt gtgcgttttc gcacgatcac acgagaagaa tttgaggcga
     1321 tgcgtccatg attggtaggg cgacgctctg cggagccgtg gaattttctc tgtggttgcg
     1381 cccgcatttg gtttcgtcgg ctcgactgag tctcgcccta ccagtgcgac tgaagcaatg
     1441 agcctgaaga ttgtccgctc tggtttccta acaaccgtcc aggatttggg tcgggtgggc
     1501 tttcgccaat tcggtgtttc cagtggtggc gcgcttgatc ttcatgcgat gcgaatcgct
     1561 aacttgctgg ttggaaatga ggagagcgcc gcgggattgg aaatcacgca aggaaatgtt
     1621 cgtttacgtt tcgacgatga gcgcatcatc agttggtgtg gcggcgatta tgaatttcga
     1681 atcgcgtcct atgcgattcc ggccggacat tcggcgcgca tttcagcagg ggatgaactg
     1741 tcgatctgcg gaccgcaacg cggttgccgt gcctggattg caatttccgc aggtattggt
     1801 gttccgagcg tcttggggag tcgctccacc gatttgcgcg gaaatttcgg cggcctgcac
     1861 ggccgcgcgg tgcgggatgg cgattcactt ccgcttggca agaattcgaa gcgcacgaaa
     1921 atttggatgg aagatctgcg agcggaacga atcgcccggt ggagcgcgcc tgaaccctgg
     1981 tcgcaaacgg cgacagctgc tcccatgctg cgcgttatcc gcggcgccga ttggaaccga
     2041 tttaacccgc cttcgcctgg ctacggcgtg gcaggcgatg tatcgattca acgttttacg
     2101 agtgaagcat tcaccgtctc tcccgattca gatcgcatgg gcgtacgcct ggccggccct
     2161 gaattgcatc gcactaacga taccgagctt atttccgagg cggtcgcgcc ggggacgatc
     2221 caagttccac ccgacgggaa gccgattctt cttttaggag attgccaaac gatcggcggg
     2281 tatccgaaaa tcgcacacgt gattacagtc gatctttcca ttgcagccca actccgtgcc
     2341 ggcgatcaag ttcgcttttc cgaagtctcg ctggccgatg ctcatggact gttgctggag
     2401 cgtgagcgtg atttcgagcg tttccgcatt ggtctcagtc tgctcgcgtc tcgtgattcg
     2461 agttgtagcc acggccctgc gggccgttcc ggcgcgagca acggcagagc gggaggcaga
     2521 cgggccacag gcccgtggct acagggcacg tgatgaaact atctgtcgat attaacgctg
     2581 atctcggcga aggcgcagga cacgatgccg agctgttcga gctcatcagc tcggccagca
     2641 ttgcaactgg ctttcacgcc ggcgatgccg atacgatgca cgccgcggtc ttggaggcga
     2701 aggcccacgg agtggctgtt ggagcgcatc ccagcttttt cgatcgggaa aatttcggac
     2761 gtaaggaact gaaagtaagt aatcaagaag tgttcgatgc cgtggcctat cagctcggaa
     2821 tttttcaggc gatcgctgtg gcggctggac tggtgccgaa ccacgtcaaa ccgcatggtg
     2881 cgctttacaa tatggcggtt cgcgatggga aactggcaga ggcgatcgcc cgggccatta
     2941 aatcggtcga ttcgaaatta attctgtttg ccccggacca taccgatctg tcccgtgccg
     3001 gcgaagcgct ggggttgcaa atcgcacgcg aagtttttgc cgaccgcaat tatctggcag
     3061 atggctggct tgttccgcgt actcgagcgg atgcgctgtt gaccgatccg gccgaagcgg
     3121 ccaaacgcgt tttgcggatg ctgcgcgaag gcaaagtccg gtctgtggaa ggcgatgacg
     3181 tcgaggtgcg cgtcgaaacg atttgcgttc acggcgacac gcccggcgca gtggagttcg
     3241 cgcgtgaatt gcgatcgcaa ctcgaatctc agggcgtcag gatcagcgcg ccgcacagag
     3301 agttgtgagc aacgacatcg agcttcaaaa acagtcaggg ctggttcggg ctcttgggcc
     3361 gctcgacgcg accatgatcg tgatgggctc gatgatcggc tcgggtattt tcattacctc
     3421 ggctgagtcg gcccggttga tcggcgctcc cggctggttg ttaatggcgt gggcagtggc
     3481 cggtctgatg acaatcaccg gcgcactctg ttgctctgaa ctggcgacga tgatgccgcg
     3541 cgcgggcggc gtttatgttt ttctccggga agcctatggg ccgtcggtcg gtttcttgtt
     3601 tggatggact ttgttcctcg tgattcaaac gggaacgatc gccgcagtgg caatcgcctt
     3661 tgcgaaattt ctcggtgtct ttgttccggc agttgggccg gagaattacc tggtgagacc
     3721 aattccgtta ggcgctcact acgccatcag tttatcgacc gagcaactga tcgcgatcgc
     3781 tttgatcgca ttgctgactt ggaccaatac
//
LOCUS       DASQUQ010000119         6737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_933477, whole genome shotgun sequence.
ACCESSION   DASQUQ010000119 DASQUQ010000000
VERSION     DASQUQ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933477"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..775
                     /locus_tag="VE486_03360"
     CDS             17..775
                     /locus_tag="VE486_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZA38155.1"
                     /translation="MISPLWQVVAAYAFALAGGSISTSLRLEHKQLCALISLAAGTLL
                     GVTLFAIAPESLDATNWWTALLAAATGYALFFFISKYVHHVCPACAASHFDADATRHF
                     SEIATALMIALAIHSTTDGLALGIQGKTPALDATKWSLFAALCIHKVPEGLALGSLLI
                     GAGLKRSGALGWVATVEATTLLGGVIGFFFLANISTFWLGLILAHVGGGFLYLAAHAV
                     LGEMLKHGKKLVLTSFLMGVALIAVLNVGLRMLG"
     gene            962..2335
                     /gene="lysA"
                     /locus_tag="VE486_03365"
     CDS             962..2335
                     /gene="lysA"
                     /locus_tag="VE486_03365"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZA38156.1"
                     /translation="MPLDSQVHFFHYRYGRLHCEEVDLERAAEKFGTPLYVYSAGTIL
                     DHYTRLDAALALLDHLICYAVKANSNRAILRLLADAGAGFDIVSAGELHRVLAAGGEP
                     AKCTFAGVGKSGEEIEYALDQRVYSFNVESEAELEYIDRIAGTKNLCAPVAMRVNPDI
                     DPRTHEYISTGSRENKFGIPLDRIHAVYERAAKMHNIDIVGVQMHIGSQITEAAPFAE
                     AIRKVAPIVRDLKSRYPIKFFSIGGGMGIIYRRALESGSGKWWHDHGGESSAFSIRDY
                     AEAIVQPLRELGIRILVEPGRFLVGNAGVLLTRIRYIKHSGEKKFAIVDAGMNDLIRP
                     ALYHSYHEIVPVEEPITRSVGEAVSFPSSKSKTEKIDIVGPVCESGDFFALDREMPEV
                     LEGDLLAIMSAGAYGFAMASNYNSRPLPAEALVHGDKVALIRKRQTWEDLVRDEVEPA
                     WSDGVME"
     gene            2431..3126
                     /locus_tag="VE486_03370"
     CDS             2431..3126
                     /locus_tag="VE486_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38157.1"
                     /translation="MKRLLIIFSLLALLQTSQARQIEGEWVIIVGGPSMHQWEKYKAY
                     PHDHWWANFVRAARIRTEQLRAERGADALITWLVYKQGYIDRAQQENQDLISHINSVR
                     DKFNLNLIYFHAGSEVINYLNYGRPRDQIKIAGLEFFGHSNRACFMFDYSNVLDSASK
                     SWLHEDELTKINRRDFARGAYVKSWGCHTGESMSKKWHAATGTRMIGAIGKTQYMMEE
                     LPILSSANAKWVN"
     gene            3226..4191
                     /gene="bioB"
                     /locus_tag="VE486_03375"
     CDS             3226..4191
                     /gene="bioB"
                     /locus_tag="VE486_03375"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HZA38158.1"
                     /translation="MNFAKLSTLYHQPLFDLISQSRAVHLQYWHGEEVQRCSLLSIKT
                     GGCSEDCAYCAQSSHYSTGVEREDLLSLENVLIAARRARSQGATRFCMGAAWRGVRDG
                     SEKFERVLEIVREVSQLDMEVCVTLGEIGPVEARKLKTAGVTAYNHNIDTSPEFYSQI
                     VSTHTFQNRLNTIAAVQESGMSVCCGGIIGMGESEADRLRMLEVLSNFDPPPESVPIN
                     CLMAMPGTPLADQPPVDIFELVRLIAVTRIALPKARVRLAAGRTRISREGQALCFFAG
                     ANSIFYGDKLLTAKNPAADVDVALLRELGMHVESMSNDECLITKE"
     gene            4444..4854
                     /locus_tag="VE486_03380"
     CDS             4444..4854
                     /locus_tag="VE486_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38159.1"
                     /translation="MNWKKLFIAFIAAFVFMFAFGFAWFGTLMHGAHQEVPVLFRTEP
                     DFPWLILGQIITAFFLAMLYAKFVPGGGAGAGATLGILVALVFAGNDLIAFAVHPLTT
                     KILCGWIVGDVIMFGIAGAIIGAIYKAAPAQTMP"
     gene            5124..6158
                     /locus_tag="VE486_03385"
     CDS             5124..6158
                     /locus_tag="VE486_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HZA38160.1"
                     /translation="MTAQTPAALGYRMPAEWEPHIATWLSWPRREGISFPDSFDGVVP
                     ALRAMVEALIESEQVCINVCNDAHEAEARQVLRGLPMKRISFHMIPTNEPWCRDHGPI
                     FLTREVDPKLAIVDWDYNAWGNKYPPFDLDEVVPTRVAQIFGLQVFNPGMILEGGAID
                     VNGAGALLTTESCLLNPNRNPNLTRDEIEQRLRDFLGVHEIFWLGSGIAGDDTDGHID
                     DLTRFVSERSVVTVVEGNRDDENYEPLQQNLGRLRQMKIEGSKIDLVTLPMPKKIVRE
                     GLRLPASYANFYIANSCVLVPTFADPADEAALSILQKCFPDRRVIGIDCRELIWGLGT
                     FHCLTQQQPA"
     gene            complement(6255..6545)
                     /locus_tag="VE486_03390"
     CDS             complement(6255..6545)
                     /locus_tag="VE486_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZA38161.1"
                     /translation="MDPARPNQPEPSDPFNPERADHAILETIEIATEGEDCDECVRKL
                     RPILMKIRGVQDVMVDLGHERVMVTFDARKTHAPDLHDAILKSGYKPGLNAD"
BASE COUNT         1417 a         1833 c         1918 g         1569 t
ORIGIN      
        1 gatcgcaagc tcctgaatga tcagtccact ctggcaggtc gttgcagctt atgcgttcgc
       61 tctggccggt ggctccatca gcacatcgct gcgtttggag cataagcagc tttgtgcgct
      121 gatcagtttg gcggctggaa cgcttctggg agtaacgctc tttgcgattg cgccggaaag
      181 tttagacgcc actaactggt ggacggcgct tctcgcggcg gccacaggct acgcgctttt
      241 cttctttatc agcaaatacg ttcatcacgt ttgtccggca tgcgcggcga gccatttcga
      301 tgcagatgcg acccgacatt ttagcgaaat cgcgacagcc ctaatgatcg cgctggcaat
      361 tcacagcacg accgacggtt tggctttggg aattcaaggg aaaacacctg ctctggacgc
      421 gacaaaatgg tcgttgttcg ctgctctgtg cattcacaaa gttccggaag gccttgcgct
      481 cggcagcctt ttaattggcg ctggattgaa acgcagcgga gcgctcggtt gggttgcgac
      541 agttgaagca accacgcttc tcggtggtgt gatcggcttt ttcttcctag cgaacatttc
      601 cacattttgg ctcgggctaa tcttggcgca tgtaggcggc ggatttctct atctggctgc
      661 gcacgccgtg cttggagaaa tgctgaagca tggaaagaaa cttgttctga caagtttcct
      721 gatgggagtg gcgctgatcg cagttctcaa tgtcggcttg aggatgcttg ggtagcgcgc
      781 gcgtcccgcg tgccagcgac ggtgtcccgc cgtcgcgaac tttttcttgc agcttggtca
      841 tggagcaaag gcacactcat ccctaattga gccgcctctt ttgaaagttc atttcggcga
      901 ggacgccgaa accagcacgc gagacgcatg cgctaccccg cgatttggaa atcggcgctc
      961 cttgccgcta gattcgcagg tgcatttttt ccattatcgc tacgggcgcc ttcactgcga
     1021 agaggtcgat cttgagcgcg cagccgagaa atttggcacg ccgctttacg tttacagcgc
     1081 ggggacaatt ctcgaccact atacacgcct cgatgccgcg ctcgcgctgc tcgatcatct
     1141 gatttgttac gcggtcaaag cgaactcgaa ccgcgctatc ttacggttgc ttgccgacgc
     1201 gggagccggc ttcgacatcg tatccgccgg cgaacttcat cgcgtcctcg ccgccggggg
     1261 cgagccggcc aagtgcacgt tcgccggcgt gggcaaatca ggtgaggaaa tcgaatatgc
     1321 cctcgatcag cgcgtttaca gtttcaacgt cgagagcgag gcggagctgg aatatatcga
     1381 tcgcatcgcg ggcacgaaaa atttgtgcgc gccggttgcc atgcgcgtca atcccgacat
     1441 cgatccacgc acgcacgaat acatctcgac cggctcgcgc gagaacaaat tcggcatccc
     1501 gctggatcgc atccacgcgg tttacgagcg cgccgcgaag atgcacaaca tcgacatcgt
     1561 cggcgtccag atgcacatcg gctcgcaaat cacagaggct gcgccattcg cggaggcgat
     1621 aagaaaagtc gcgccgatcg tgcgcgattt gaaatcacga tacccgatca agtttttcag
     1681 catcggcggc ggcatgggaa tcatttaccg gcgcgcactc gagagcggat caggcaaatg
     1741 gtggcacgac cacggcggcg agtcgtccgc gtttagcatc cgtgattacg ccgaggcaat
     1801 tgttcaaccg ttgcgcgagc ttggcattcg gattttagtc gagcctggtc gctttctggt
     1861 cggaaacgcc ggtgtcttgc tcacgcgcat tcgctacatc aagcacagcg gtgagaagaa
     1921 attcgcgatt gttgatgcgg gaatgaacga cctgattcgg ccagcgcttt accatagcta
     1981 tcatgagatc gtgccagtgg aagagccgat cacgcgctct gtaggggaag ctgtcagctt
     2041 cccgtcgagc aagagcaaaa cagaaaagat cgacatcgtc ggtccggttt gtgagagcgg
     2101 agattttttt gcgctcgacc gcgagatgcc cgaagtgctt gagggcgatc tgctcgccat
     2161 catgagcgcg ggcgcctatg gatttgcgat ggcttcaaat tacaattcgc gtccgctacc
     2221 ggcggaggcc ttggtacacg gcgacaaggt agcgctgatt cggaaacggc agacgtggga
     2281 ggatctggtg cgggatgagg tcgagcctgc ttggagtgat ggagtgatgg agtgatgctc
     2341 atttccatta ctccactgct ccattactcc aacacgcctg ccgcttgcag atttcgtctt
     2401 ccgcataata gtccttccac tgttaaatcg atgaaacgac tgctcatcat cttttcgttg
     2461 ctcgctctcc tccagacatc gcaagcccgc cagattgaag gcgaatgggt catcattgta
     2521 ggcggcccct caatgcatca gtgggagaaa tacaaagctt atccgcacga ccattggtgg
     2581 gcgaactttg ttcgcgccgc gcggatacgc actgagcaat tgcgcgccga gcgcggcgcc
     2641 gatgccttaa tcacctggct cgtttataag caaggctata tcgatcgcgc tcagcaggaa
     2701 aaccaggatt tgatcagcca catcaattcc gtgcgcgata aatttaacct gaatctgatt
     2761 tattttcacg ccggatcaga agtcatcaat tacctcaact acgggcggcc gcgcgatcag
     2821 ataaagattg ctggcctcga attttttggg cattcaaacc gagcttgttt catgttcgat
     2881 tacagcaatg tgctcgacag cgcatcgaaa tcgtggctgc acgaggacga gttgaccaag
     2941 atcaatcggc gcgatttcgc gcgtggcgcc tacgtcaaga gctggggctg ccatacgggc
     3001 gagagcatgt cgaagaaatg gcacgctgcg accggcacgc gcatgattgg cgccataggc
     3061 aaaacgcagt acatgatgga agaactgccc attctcagtt cggcaaacgc caagtgggtg
     3121 aattgaatgt ttgatgtttg actgagcaac ggcgctcttg tcatttcgag cgaagtcgag
     3181 gaacctctca ctgttattct aaacccgctg gtttgcctgc gcgctatgaa cttcgcgaag
     3241 ttatcgactc tctatcatca gcctctcttc gatttgattt cccagagccg ggcggttcat
     3301 ctccagtact ggcatggcga agaagtacag cgttgcagtt tgctcagcat caagacgggc
     3361 gggtgcagcg aagattgcgc ttattgcgcg cagtcctctc attattctac tggagtcgag
     3421 cgggaagact tgttgtcgct ggagaacgtt ctgatcgcag ccaggagagc gcgttcccag
     3481 ggcgcgaccc ggttttgcat gggcgcggcc tggcgcgggg tgcgcgatgg ctctgaaaaa
     3541 tttgagcgtg tgctggagat cgtgcgggaa gtaagccagc tcgacatgga agtatgcgtc
     3601 acgctcgggg aaatcggtcc ggtggaagcg cgtaagctga aaaccgctgg cgttaccgct
     3661 tacaatcata acattgacac atcgccggaa ttttattccc agattgtgag cacacacacg
     3721 ttccagaatc ggcttaacac gattgccgcc gtgcaagaga gcgggatgtc ggtttgttgc
     3781 ggtgggatca ttggcatggg cgaatcggag gccgatcgcc tgcgcatgct ggaagtattg
     3841 agcaatttcg atccacctcc ggaaagtgtg ccgatcaatt gtttgatggc gatgcccggt
     3901 acgccgctgg ccgatcagcc accggttgat atttttgagc tagtccgcct gattgccgtc
     3961 acgcggatcg cgttgcccaa ggcgcgggtt cgtttggcgg ccggccgcac aaggatctcg
     4021 cgcgaaggtc aggcgctttg cttctttgcc ggtgcgaatt ccatttttta cggcgacaaa
     4081 cttctgaccg cgaaaaatcc ggccgctgat gttgatgtcg cccttttgcg ggagcttgga
     4141 atgcatgtgg aatcaatgtc gaatgacgaa tgtctaatca cgaaggaatg acgaaatccg
     4201 aatgacgaaa taaacacgga tgccgtgtaa ttaggcttcg tacttcgtca tcaattcagc
     4261 attcgtcatt cgtgcttcgc catttccgcc tccatctttc gcctgtcacg ccactacggc
     4321 ccgaacgcga gcacgttatt ttgcgcagtc ccaccaaaat tttcggatcg acattggctg
     4381 gtcattttgc aatgaaaggc gtcgggactt ggggatcaac aaaaacctca atagaaagaa
     4441 attatgaact ggaaaaaact cttcatcgca ttcatcgcag ccttcgtttt catgttcgcg
     4501 tttggcttcg cttggttcgg gacgttgatg catggggcgc atcaggaagt gcccgtgctt
     4561 tttcgaaccg aacccgattt cccgtggctg attttgggtc agatcatcac ggcgtttttt
     4621 ctcgcgatgc tttatgcgaa gttcgttccg gggggcggcg ccggtgccgg cgcgacattg
     4681 ggcattctgg ttgcgctggt gtttgcaggg aacgacctga tcgcgttcgc ggtgcatccg
     4741 ctaacgacga agattctctg cggctggatt gtgggagacg tgatcatgtt cggcattgcc
     4801 ggcgcaatca ttggcgcgat ctacaaagct gccccggcgc aaacgatgcc ttagagctga
     4861 atctcgcctt cgcatacaca gcgccaggaa gagacggacc gccgggcagg gcgacccctt
     4921 ttgaccgggg agcgcaggct gccagcctgc agttgctggc agcttgccgg caacactctt
     4981 gcggaagtga accggcgtaa cacacatcgt ctcggcaaac tgccgagatg aacaggcttg
     5041 gagcctgcgc tccccagaat gtcatatcgt cgacgcaatg ccgcagagat tgcgatttta
     5101 gctgggcgtt ggacgtttgc tgcatgactg cacagactcc tgcagcgctg ggctatcgca
     5161 tgccggcgga atgggaaccg cacattgcaa cctggctttc atggccgagg cgcgaaggaa
     5221 taagcttccc cgattcattc gacggcgtcg tgccagcttt gcgggcaatg gtggaagcgc
     5281 tcatcgaatc agagcaagtt tgcatcaacg tttgtaacga cgcccacgaa gccgaggcgc
     5341 gtcaggtcct ccgcggatta ccgatgaaac gaatctcgtt tcatatgatc ccgacaaacg
     5401 agccgtggtg tcgcgatcac gggccgattt ttttgacgcg cgaggtcgat cccaaactcg
     5461 cgattgtcga ttgggattac aacgcctggg gcaacaaata tccgccattc gatctggacg
     5521 aagtcgtgcc gacgcgcgtc gcccagattt tcggcctcca ggtttttaat ccgggcatga
     5581 ttttagaagg cggcgcaatc gatgtgaatg gcgccggcgc gcttctcacg accgaatcgt
     5641 gtctgctcaa cccgaatcga aatccgaatt tgactcgcga tgaaatcgag cagcgcctcc
     5701 gggattttct cggcgtgcat gaaattttct ggctcggcag cggaatcgcc ggcgacgaca
     5761 ccgatggcca cattgacgat ctcacccgtt ttgtgtcgga gcgctccgtc gttacggtcg
     5821 tcgaaggaaa tcgtgacgat gaaaattacg agccgcttca gcaaaacctc gggcgtttac
     5881 gccagatgaa gatcgaaggg agtaagatcg acctcgtcac attgccaatg ccaaagaaga
     5941 tcgtccgcga agggctgcgc ttgcccgcaa gttacgccaa tttttatatc gcgaattcgt
     6001 gcgtcctcgt gccaaccttc gccgatcccg ccgacgaagc cgctttatcg atcttgcaaa
     6061 aatgtttccc ggaccgccgc gttatcggaa tcgattgccg cgaactgatc tggggactgg
     6121 gcacgtttca ctgtctcacc cagcagcagc cagcctaatt tctgtagcgg cgctctatga
     6181 gcgtcgaatc gcttaatttc ggcggtcatc cgcctgggtc agactacggc gcgacaagta
     6241 gaccggctac ggtattaatc cgcgttcagt ccgggcttgt agccgctctt caaaattgcg
     6301 tcgtgaagat cgggcgcgtg cgtcttgcgc gcatcgaatg tgaccatgac gcgttcgtgc
     6361 ccgagatcga ccatcacatc ctgcacgccc ctgattttca tcaggatcgg ccgcagcttt
     6421 cgcacgcatt cgtcgcaatc ttcgccttcg gtggcgattt cgatcgtttc caggatcgcg
     6481 tgatcggccc gttcgggatt aaacggatca ctcggttcag gctggtttgg tcgcgctggg
     6541 tccatcgttt ccttatctaa cgtgtgggaa tagcgctgcg gtcgtctcac acgcgtcgtc
     6601 atcctgaatc cgcctgaggg ggatgaagga tctcacaatg acacctgcat ctccgatggg
     6661 gctctcgaaa atcaatgatc caatgtgagg tccctcgcca tcttcgcagc tcgagatgac
     6721 atcacgccga tttcgcg
//
LOCUS       DASQUQ010000120        13189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1109229, whole genome shotgun sequence.
ACCESSION   DASQUQ010000120 DASQUQ010000000
VERSION     DASQUQ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109229"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VE486_03395"
     CDS             <1..667
                     /locus_tag="VE486_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZA38162.1"
                     /translation="WRTNVPVPITVRLPSGGTKGSGPFHSQSMESLYAHYPGLIVMTP
                     ATVEDAYTMLIEAVAIDDPVIYCEHKYLYYHLKADDLPTEGLPTGKARIAREGRDLSI
                     VTYSAMVHEALAVAEQLAGEGFEIEVVDLRSVKPLDTDTVLASVARTGRLLCVGESFP
                     WGGATAEIIARVTAEGFHLLDASPQRLNAKDTPIPYHPNLWSAHRPNAKEIARKARAL
                     LRE"
     gene            672..1895
                     /locus_tag="VE486_03400"
     CDS             672..1895
                     /locus_tag="VE486_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZA38163.1"
                     /translation="MPQVPIIMPQLGESIAEATIVDIKVKPGDEVVDDQEIIDVETNK
                     AVMGVTTPCKGKIDKIAVQLKETYPVGAVLGYVEASEADAARFTKQAPPSPQQEVAEE
                     IPARADQEVMPVRQQKVAARGRDGERAGGLPVPVAPKGASFMSPRVRARMAELQLTTA
                     DLAGIMGTGAGSRVTIKDLENFLHKMENQTAHEASAIRAGVADAMRRSWTRPLSTIGS
                     SVNLDPVLQDRQSRDPKPGPGLYALRALALALAENPNAAAKLIGNRVVQSTSIDVGIA
                     VEAEDGIMVPVIRAADNTSLADLAAKYKELVDLARQRRISPDMQAGGIATVSNFGTFG
                     MEWGTPIPLPDQTLLLGLGAGKKVPSWDEEKKQFVPKTEAQITLSFDHRSIDGGGAGR
                     LLKRVIELVEDPKKL"
     gene            2124..3155
                     /locus_tag="VE486_03405"
     CDS             2124..3155
                     /locus_tag="VE486_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38164.1"
                     /translation="MTRRIIIHAGFPKCGSSSIQAALFHNFARLQKDGISLFGKELRI
                     AHTSADLGVPLWFLEHAKEKSEPLAQRLSDEIASVGDGNGDRIGILSAENLSRPEMAQ
                     LFASLDSRLEVSLVFYLRPQREWIPSAWKQWDLKRGMSLSDFVSRCIRIRRPPFKLGI
                     ETWRSALPAAKIHVRFLIPELLIGGNPAADFFDLVGLSRDRYLIEEEPRNPSLDYSIL
                     HVLSKNPHLFSSIHDNKLMKGLMRALPAKFQSTNIQMLSREEEARIEESFRDENLWLL
                     NTYCSGIDADRIYRTYFTPQEAEARYSSATDLDLIYRCLGIILESIAASSEATAADDK
                     ISGTPKQES"
     gene            3326..3835
                     /locus_tag="VE486_03410"
     CDS             3326..3835
                     /locus_tag="VE486_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38165.1"
                     /translation="MNSRSSPPAAGAGERWRFLRDVVVFQLKLLLDNLRDLALVPISL
                     VAALIDLVYKGEREGALFYRVLRWGAHSEEVIDVYSAIEHHAPSSFKVNPAYTVDAVV
                     ARLEGVLVREYEKGGTAASIKAAMDRAIDQVHRETRDKGDPARDVVARAADKLRLKID
                     SLPTEKDEG"
     gene            complement(4038..4436)
                     /locus_tag="VE486_03415"
     CDS             complement(4038..4436)
                     /locus_tag="VE486_03415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZA38166.1"
                     /translation="MSSAVFEMTVPVLPNDIDEQNHVNNTVYLRWVQDVATAHWQAIA
                     SAEVQEAIGWVVLRHEIDYKMPATLGDEIVLRTWVGKATRLTFERFTEIRRKSDRQLL
                     SQARTLWCPINTQTGRPVRVPAEVRAQFSI"
     gene            complement(4567..4782)
                     /locus_tag="VE486_03420"
     CDS             complement(4567..4782)
                     /locus_tag="VE486_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38167.1"
                     /translation="MTKTEITVWVISALLAIGVGWSRYAKMKTRDKLVSELAAMDPER
                     REKVLSRLNPKLALEMREHLMKRFGIS"
     gene            4928..5731
                     /locus_tag="VE486_03425"
     CDS             4928..5731
                     /locus_tag="VE486_03425"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HZA38168.1"
                     /translation="MNEREQEIASGLPPDLDTLPWLGGFRLRGIEMTRLETFIDAAFA
                     FAITMLVIAAERIPDDIETLLAAFKNVPAFVASIIVLGIFWRGHWLWSRRYGLEDGVS
                     IFISWAMIVTILIYMYPLKAIFSSMWFLLSGGRVGHTLGAHSAWQVRVLFAVFAVGFT
                     AIALEVVLLNLRAWQLREPLQLNAKERLITFHEVTGWSVPVAVGIISFVLALSLPREQ
                     IAWSGWIYFSMVILVPLHSAYRKRRVEAAKPRRSDPPTREPTARQVGKR"
     gene            5808..6242
                     /locus_tag="VE486_03430"
     CDS             5808..6242
                     /locus_tag="VE486_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38169.1"
                     /translation="MKQFLGVVVTLLASLSPVWANLGDGYERLDDAYGNLVERHLLDD
                     GTVSVVYKKDRYFYFVIFDGGRSVLERYSHVKGTDLSEKEIARFLKAHAGGETWITTD
                     KPKERRFKRSDGKVEATYATVDGRPTLTVREVRAGRSQRSDQ"
     gene            complement(6324..6908)
                     /locus_tag="VE486_03435"
     CDS             complement(6324..6908)
                     /locus_tag="VE486_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38170.1"
                     /translation="MKILLAGILGGIAMFIWTSIAHMFLPLGEAGISEIPNESAVLSA
                     MQSNIGDQTGLYIFPGPGVGKNATRQEKNEAMKKMPEKMAANPSGILMYHAPGRPFTF
                     GKWLGVEFATELLEAILAVFLLAQTAIVSFAGRVGFVLVAGILAAIATNVSYANWYGF
                     PCVYTASYMSIQIIGFLVVGIVAALVLPKRSPAG"
     gene            complement(6971..7429)
                     /locus_tag="VE486_03440"
     CDS             complement(6971..7429)
                     /locus_tag="VE486_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HZA38171.1"
                     /translation="MKALSTSKAQKVLPPIEVRPSGVHGRGVFATQFIPRDTRIIEYT
                     GQRVPWESAPNDENNPHTFNFGLENGVVINPEIGGNDARWINHCCDPNCEAIEEDDRI
                     FIYAIRDLEPGEELFYDYALEIDEPITEESKKEFACHCGSPDCRGTMLSL"
     gene            complement(7451..7822)
                     /locus_tag="VE486_03445"
     CDS             complement(7451..7822)
                     /locus_tag="VE486_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZA38172.1"
                     /translation="MTTESPTINYKIGNERLVQVTEKAARKLASLLEQKGNPNGALRL
                     AVVGGGCSGLQYVMDLVDGPKERDILVPTSNVRLVVDPKSALFVSGSVLDYSEDLQKG
                     GFKVTNPNAVAHCSCGESFAV"
     gene            complement(7856..8365)
                     /locus_tag="VE486_03450"
     CDS             complement(7856..8365)
                     /locus_tag="VE486_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38173.1"
                     /translation="MNPLSDEWAIKHRADACAATHRPFAPGEYFYTLLFHDADGYRRE
                     DLSEEAWRNRNENIQPFSFWKSRYEPLPAAPPEPLPKENAEQLFRRLIASENPPANAC
                     YVLAAMLERKRILKQIKTEETANRRVLIYEHGGTGDVFIVPDPRLRLDELESVQNEVV
                     QLLHAEARD"
     gene            complement(8362..9501)
                     /locus_tag="VE486_03455"
     CDS             complement(8362..9501)
                     /locus_tag="VE486_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZA38174.1"
                     /translation="MRLLDRYVVRNFLQVYLYCIAGFISIWLIFDISDNISTFIEQHI
                     GLPVVAQYYATQVPQIFIILLPVSLLLALLFTLGRMSRANEIVSMLTAGISLPRVLLP
                     LMGIGLLTVGVSMALNYSLAPHAELARKALLSQARSRPEASIEGQIFRNRTDARTWFI
                     QNFRLGGNAFNNVQVLQQDANDNIVTNYVATRAFYRPETKTWDLENAKVVHYDHAGNI
                     VDEEIYPSLKVEHWSETPFRLGSANVRAEFLSLPELREYLHFNSDFPPTLLAPFRTHL
                     QYRLALPWTCLVVVCIAAPLGIGYSRRGVLSSVAAAVVLVFSMNFLVQLFLALGEGDR
                     VSPWIAAWTPNLLFTAIGLYLFYLRASNREAPGFHLFTARRIVAQ"
     gene            9710..11692
                     /locus_tag="VE486_03460"
     CDS             9710..11692
                     /locus_tag="VE486_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HZA38175.1"
                     /translation="MIALKDSLGTEFVESVRAFFSENGPLSKAKNFEFRLQQQEMAAA
                     VAKALEEERHLVVEAGTGVGKSLAYLTPAILFALEQHKKAIVSTHTINLQEQLLYKDI
                     PILKKILPVEFDAALMKGRQNYLCPRRLERALQQQNELFTGPEQNELTRLAEWARTTR
                     DGSLSDLPIEPDPKVWVQVCSEPHICTVKTCGQGPGCFYQQARKRLLSSDVIVINHTL
                     LFMLLGSPDQQEERESGYLFPNDFIIFDEAHTVERVASRQIGIGISQYGLRSTVQRLY
                     NARTRKGLFTVMRDAIGVRLAAEAVDELDKFFGAVESRSDFRKGREFRVRAPELVPDT
                     ITGRLTGLQARIAEVVRRADDEFLKAELQELGRRIGDARDGIAIFLEQSAREHVYWVE
                     RTGKTAQFLSLNAAPIDMAPVLRRMIFREGCSCVMTSATLSVGRPDLAYFRRRIGADE
                     VEPLLLGSPFDFRTQMKLFIVQKMPDPRDATYEKALEHWIAHFIEETDGRAFVLFTSY
                     RGMQQVADQMGEFFSRKKFNLLVQGGGAPRGRLLEQFKTTPRSVLFGTDSFWMGVDVP
                     GEALSNVIITRLPFAVPDHPLIEAKLELVEERGGDPFTEYSLPEAVLKLRQGVGRLIR
                     TKTDRGIIVILDNRIVSKPYGRAFLQALPKCPVKII"
     gene            11738..12814
                     /locus_tag="VE486_03465"
     CDS             11738..12814
                     /locus_tag="VE486_03465"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38176.1"
                     /translation="MKRSSGSNSAAAKNREAEVRQILAILHKWGIHTLGQFAALDKEQ
                     VNARLGLEAVRIWERANGQSDRPLKLVRPPESFEESFEFENEIETAEPLLFMLRRFLE
                     QLAVRLSAIYLVAKELTLRIIFGNKQTYERLFKIPQPTNDVDLLFRMLQTHLENFKSE
                     HPIVAVALSAQPIKPEKEQFGLFETTLLNPQQLSETLARLTALLGADRVGTPVLDETY
                     RPDAFHMEPFCSTGFQPVGQAGVLACERTGRQDAPRPHSQDGCATSKASLRRFRPAPS
                     ASVFLFENIPAHIRSADIRGKVVQQRGPYLSSGNWWDEKSWARAEWDLQIEDGELVRC
                     HESPPSSNYGAIGEGTWEMDGLYD"
     gene            12835..>13189
                     /locus_tag="VE486_03470"
     CDS             12835..>13189
                     /locus_tag="VE486_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZA38177.1"
                     /translation="MTMSYIELHACSAFSFLRAASFPEQLAEVGAELEMPALALLDRN
                     GVYGAQRFSVAAREHGVRPIIGAELSMEDGGILPVLVENRTGYKNLCELLTQAHLRSE
                     KGNCAIQWNELPQFAE"
BASE COUNT         2970 a         3471 c         3915 g         2833 t
ORIGIN      
        1 ttggcggacg aatgttccag ttcccatcac ggtgcgtttg ccctcgggcg gaacaaaagg
       61 cagcggacca tttcacagtc agtcgatgga atcgctttac gcgcattacc cgggactcat
      121 cgtgatgacc ccagcgacgg tagaagacgc ctataccatg ctgattgagg ctgtggcgat
      181 cgatgatccg gtcatctact gcgagcacaa atatctctac taccacctga aagccgacga
      241 tctgcccacc gaaggcttgc ccaccggcaa agcgcgcatt gcgcgggaag ggcgcgacct
      301 ctccattgtt acttatagcg cgatggtgca cgaagccctt gcggtcgcag agcaattggc
      361 gggcgaaggt ttcgaaattg aagtcgtcga tctccgctcg gtcaaaccac tcgataccga
      421 cacggttctt gcttcggtcg cgcgcaccgg gcgcttgctt tgcgtcgggg aatcatttcc
      481 gtggggcggc gcgacggctg aaatcatcgc gcgcgtgacg gcggaaggtt ttcatttgct
      541 ggatgcctcg ccacagcgcc tgaacgcgaa agacactccc atcccgtatc acccaaatct
      601 ctggtcggca caccggccga acgcaaaaga gatcgcgcga aaagctcgcg ccctgctccg
      661 agaataaatt tatgccgcaa gtcccaatca tcatgccgca gctgggcgaa tccatcgccg
      721 aagccaccat cgtcgatatc aaagtgaagc ccggcgacga ggtggtggac gatcaggaaa
      781 ttatcgatgt cgagacgaac aaagccgtca tgggcgtgac cacgccgtgc aaaggcaaga
      841 tcgacaagat cgccgtgcag ctaaaggaaa cctatccagt gggcgcggtg ctcggctacg
      901 ttgaagcgag tgaagcggat gccgccagat tcacgaaaca agcgccgcct tcgcctcaac
      961 aggaagtcgc agaggaaatt cccgcgcgcg cagatcagga agtcatgcct gtgcgccagc
     1021 aaaaagtcgc cgcccgcggc agggacggcg aacgcgctgg cggcttgccg gttccagtcg
     1081 caccaaaggg cgctagtttc atgtccccgc gggtccgcgc gcggatggcg gagttgcagt
     1141 taacgacagc tgatctcgcc gggatcatgg gaaccggcgc tggcagccgc gtcacgatca
     1201 aagatctgga aaatttcctg cacaagatgg agaaccagac cgcgcatgaa gcgtctgcca
     1261 ttcgcgccgg tgtcgcggat gcgatgcgcc ggagttggac ccgcccgctc tcaacgattg
     1321 gttcctcagt gaatctcgat cccgtcctgc aagatcggca atcgcgcgat ccaaaaccgg
     1381 gtcccggcct ttacgcgctg cgcgcgcttg cgctcgccct ggccgaaaat ccaaatgcag
     1441 cggcgaaatt aatcggcaac cgcgtcgtgc aatcgacgtc gatcgacgtg ggcatcgcgg
     1501 ttgaagctga ggacggaatc atggtgccgg tcattcgcgc tgccgacaat acgtctcttg
     1561 ccgatcttgc ggcgaaatac aaggagctgg ttgatcttgc gcggcagcgg cggatttcgc
     1621 cggacatgca agccggcggc atcgctaccg tttcgaattt cggaaccttc ggaatggaat
     1681 ggggcacgcc gattccgctg cccgatcaga cattgttgct cggtctcggc gcggggaaga
     1741 aagttccctc gtgggatgaa gagaaaaagc aatttgttcc caagaccgaa gcgcaaatca
     1801 cattgagttt cgatcatcgc agcatcgacg gcggcggcgc gggccgtttg ctcaaacgcg
     1861 taatcgagct ggtggaagat ccgaaaaaac tctgatctgg gtagcgcacg cgtctcgcgt
     1921 gttggcgatg gcgtcctcgc catcggaact tttccaaaac ccgacgctga ttcgatagcg
     1981 cagcataagg aaagattgct gcggcgagac accgcaacca gcacgcgaga gccagtgcgc
     2041 gatccaaaac ggtttgcgac cgcgagcggt ttacttcggc attgcatgcg aaggggtctg
     2101 gcgtcacgct tttattcggc aacatgacga gacggatcat aattcatgca ggattcccaa
     2161 aatgcggaag cagcagcatt caggcggcgc tttttcacaa ttttgccagg ttgcagaagg
     2221 atgggatttc tcttttcggc aaagagctga ggattgccca cacgtctgcc gatctcggtg
     2281 tgccactgtg gttcctcgaa catgctaagg agaaaagtga accgcttgcg caaaggctta
     2341 gtgacgagat cgcatctgtc ggggatggaa atggagatcg gatcgggatt ctgagcgccg
     2401 agaacctgtc gaggccggaa atggctcagt tgttcgccag cctcgacagt cggttggaag
     2461 tctctttggt cttctacctg cggccgcagc gtgaatggat tccatcggca tggaagcagt
     2521 gggacctgaa aagaggcatg tcgctgagcg actttgtttc tcgttgcatc cgcatacgca
     2581 gaccgccgtt caaactgggt atcgagactt ggagaagtgc gctacccgca gcgaaaatcc
     2641 atgttcgatt ccttatcccg gagctgctga tcggcgggaa tccggccgcg gattttttcg
     2701 acctcgttgg actttcccgg gacagatatc ttatcgaaga ggagcctcga aatcccagcc
     2761 tggattattc cattctccac gtgctctcga agaatccgca tcttttttcc agtattcatg
     2821 acaacaagtt gatgaaaggg ttgatgcgcg ccctgccagc gaagtttcag tcaacaaaca
     2881 ttcagatgct ctcacgcgaa gaagaagccc ggatcgagga atctttccgg gacgaaaatc
     2941 tctggctgtt gaacacgtat tgcagcggga tcgacgcgga tcgtatctac cgcacctatt
     3001 tcacgccaca ggaagctgag gccagatatt ccagcgcgac ggacctcgat ttgatctacc
     3061 gttgcctcgg gatcatcctg gaatcgatcg ctgccagtag cgaggcgact gcggcggacg
     3121 ataagatctc cggaactccc aagcaggaga gctgacctca cggtgacgta gctgccgttt
     3181 tcgatcacag cggtaagttt tggaagtttc atcacgaatt tcttatccat ccaccgggtg
     3241 ttggccaata acgccaaggg cggaaatcac gtcggacgcg cgagtgttcg tgacgcgtat
     3301 tcttttgcct ggtacaattg ccggcatgaa ttcgcgttcc tcaccaccag ctgcgggcgc
     3361 cggcgagcgt tggcggtttt tgcgggatgt cgtggttttt cagctgaagt tgttgctcga
     3421 taatttgcgc gatttggcgc tcgtcccgat ttcgctcgtc gcggcgctca tcgatttggt
     3481 ttacaaaggc gagcgcgaag gcgcgctctt ttacagggta ttgcggtggg gcgcgcatag
     3541 cgaagaagtg atcgacgttt acagcgcgat cgagcatcac gcgccgagca gtttcaaagt
     3601 gaatcccgcc tacacggttg atgccgtggt tgcgcgactg gaaggcgtac tagtgcgtga
     3661 gtacgaaaag ggcggaaccg ctgcgagcat caaggcggca atggatcgtg cgatcgatca
     3721 ggtgcaccgc gaaacgaggg ataaaggcga ccctgcccgg gatgtcgtag cacgcgcggc
     3781 agataagttg cgcctcaaga tcgacagctt gcccactgag aaggatgagg gatgaggaca
     3841 gaaatcgaag gacacccacc tacgtcccgc aaccgcggga gtacggcgag ccaaggaggg
     3901 caaatgtcag agatcgaaag tcagcggtca gcggatcagc cgccttcggt gctggcgctt
     3961 cggcgtgccg gggaggtgac gtcggcgtaa ctttaaccaa gattttccaa tgagaagcga
     4021 tttggcggcg gaccggctca gatggaaaat tgtgcgcgca cctcggccgg gacgcggacc
     4081 gggcggccgg tctgtgtgtt tatcggacac cacagggtgc gggcttgcga caggagttgg
     4141 cggtcgctct tgcgtcggat ctcggtgaag cgttcgaacg tgagccgggt ggctttgccg
     4201 acccaggtgc gcagcacgat ttcgtcgccg agcgtggcgg gcatcttgta atcgatttcg
     4261 tgtcgcaaca cgacccagcc gatcgcttcc tgaacttcgg cactcgcgat tgcctgccag
     4321 tgcgcagtgg cgacatcctg cacccagcgc aggtaaacgg tgttgttcac gtgattttgc
     4381 tcgtcaatat cgtttggcaa aaccggaacg gtcatttcga aaacggcgga ggacattgga
     4441 ggccagaggc cagaggtcac ccgcctaggt acgagccgcc ttcactagaa gttacggcga
     4501 gccatgggct atggcgagcc aaggaggtca gaggttggaa gtcagaggtc aggcgagcga
     4561 tggaagtcag cttattccaa agcgtttcat tagatgttcg cgcatttcga gtgcgagttt
     4621 cgggttgagg cggctaagca ctttttcgcg gcgttctggg tccatggccg caagttcgct
     4681 cacaagcttg tcgcgcgttt tcattttcgc gtaacgactc cagccgacgc cgatcgcgag
     4741 cagcgcggat attacccaaa cggtaatctc agttttggtc atgaaggaaa gaatgaagca
     4801 ataatcaaaa aggtgaaacg gaaaacagag gtcagaggtc agacgtcaga agccagaagt
     4861 tggagatcag cagcggcgaa aatactctac agactcgggc ccggattgca gtagcattgc
     4921 cagccagatg aacgagcgcg aacaagaaat cgcatccggc ttgccgccgg acttggatac
     4981 gttgccgtgg ctcggcggat ttcggttgcg cggtatcgag atgacccggc tggaaacctt
     5041 catcgatgca gctttcgcgt tcgcgatcac gatgctggtg atcgcggcgg agcggattcc
     5101 ggacgatatc gaaacgctcc tggccgcttt caagaatgtg cccgccttcg tcgcgagcat
     5161 catcgtgctc ggaatttttt ggcgcggaca ttggttgtgg agccggcgtt atggcctcga
     5221 agatggcgtt tcgatcttca ttagctgggc gatgattgtc acgatcctga tctatatgta
     5281 cccgttgaaa gcgattttta gttcgatgtg gtttctgctc agcggcggtc gagtgggcca
     5341 cacactgggg gctcattcgg catggcaggt gcgcgttctt tttgcggtct tcgcggttgg
     5401 gtttactgcc atcgcactgg aggtggtgct gttgaatctg cgtgcctggc aattacggga
     5461 gccgttacag ttgaatgcaa aggaaagatt gatcacgttc cacgaggtaa ccggatggag
     5521 cgtcccggtc gcggtcggaa tcatttcgtt tgtcctggct ctgagcctgc cgagggagca
     5581 gatcgcctgg agcggctgga tttacttttc gatggtcatt ctcgtgccgc tgcattcggc
     5641 ctaccgcaag cggagggtgg aggcggcgaa gccgcgaaga agtgatccgc ctacgcgcga
     5701 gcctacggcg cgacaagtcg gtaaacggtg atcagtcatc agtgatcggt ggaattgcga
     5761 aatgcgggtt tctgcgagat tgttgcaatc tcgcaagcac agctcttatg aagcaatttc
     5821 tcggcgttgt tgtgacactc cttgcgagct tgagtcccgt gtgggctaac ctgggtgatg
     5881 gctacgagag acttgacgat gcgtatggca acctggtcga gcggcattta ctcgatgatg
     5941 gaacggtcag cgtcgtttac aaaaaagatc gatactttta cttcgtcatt ttcgacgggg
     6001 gccggagcgt tttggaaaga tattcacacg tcaagggcac cgatctttcc gagaaagaaa
     6061 tcgcgagatt cctaaaggct catgctggcg gcgagacgtg gataacgacc gacaagccga
     6121 aagaacgtcg attcaagcgg agcgatggca aggtcgaggc aacgtatgcg accgtggatg
     6181 gccggccgac acttacggtg cgggaagttc gcgctggccg cagtcagcgt agtgatcagt
     6241 gatcggtgat cggaagtcag agggcggaag tcagaaatcg gattccggaa aagtttaagg
     6301 tgccaaaatg gtacctcaag atttcagcct gccgggctgc gctttggcaa caccagcgcc
     6361 gcaacgattc ccacgacaag aaatccgata atctgaatgg acatgtagct ggcggtgtaa
     6421 acacagggga agccatacca gttcgcatag gaaacgttcg tcgcgatggc ggcgagtatt
     6481 ccggcaacca aaacgaagcc gacccggccg gcgaaactca cgatggctgt ctgggcaagg
     6541 aggaatacgg ccaggatcgc ttccagcagt tcggtggcga attcgactcc gagccatttc
     6601 ccaaacgtga acgggcgccc gggcgcgtga tacatgagaa tgcccgaagg attcgccgcc
     6661 attttttcgg gcatcttttt catcgcttca ttcttttcct gccgggtcgc gttcttgcct
     6721 acgccgggtc ccggaaaaat gtaaagaccg gtctggtcgc cgatgttgct ttgcattgcg
     6781 ctgaggacgg ctgattcatt tggaatttcg ctgatgccgg cttcgccaag gggcaggaac
     6841 atgtgcgcga tcgacgtcca gataaacatg gcgatgccac cgagaatgcc agcgagaagg
     6901 attttcataa gcaggaacgc tgaaggataa aagaggaatt tagaaggaca aaatgcaggc
     6961 gcggctggcg ctacagcgaa agcattgtgc cgcggcagtc tggactcccg cagtggcacg
     7021 cgaattcctt ctttgattct tcggtgatgg gttcgtcgat ctccagcgca taatcgtaaa
     7081 agagttcctc accgggttcg agatcgcgta ttgcgtagat gaaaatgcgg tcgtcttcct
     7141 cgatcgcttc gcagttcgga tcgcagcaat gattgatcca gcgcgcgtcg ttgccgccga
     7201 tctcgggatt gattactacg ccgttttcga ggccaaaatt aaatgtgtgc ggattgttct
     7261 cgtcattggg agcgctctcc caggggacgc gttggccggt gtattcgatg atccgggtat
     7321 ccctcgggat gaactgcgtg gcgaaaacgc cgcggccgtg gacgccagaa ggccggacct
     7381 cgatgggcgg taagactttt tgagccttgg aagttgagag ggccttcaag cgacgaacgc
     7441 gcgaataagc tcaaacggcg aaactttcac cgcaggagca gtgggcgacg gcgtttgggt
     7501 tcgtcacttt gaagccgcct ttctgcaaat cttcgctgta atcaaggacc gagccgctga
     7561 cgaatagcgc gctctttgga tcgacaacca ggcgcacgtt ggacgtcggc acgaggatat
     7621 cgcgctcttt ggggccatcc accaaatcca tgacgtactg caggcccgag cagccgccgc
     7681 cgactaccgc caggcgtaac gcgccgtttg ggttgccctt ttgctccagg agcgatgcca
     7741 atttgcgcgc ggctttctcg gtcacctgga ccaggcgttc gttgccgatc ttgtaattga
     7801 ttgtcgggga ttcagtggtc attcaaaaat gataatcagg agtgcgtttg atgtttcaat
     7861 ctcgtgcctc tgcatggagc aattggacga cttcattttg cacgctttcc aattcgtcga
     7921 gccgcaatcg tgggtccggc acgatgaaaa catcgccggt tccgccgtgt tcgtaaatca
     7981 aaacacgccg gttcgcggtt tcctcggtct ttatctgttt cagtatccgc ttacgctcaa
     8041 gcatggcagc gaggacgtag cacgcattcg ccggaggatt ttccgatgca atcaaccggc
     8101 ggaagagctg ctcggcattt tcttttggaa gaggttcagg cggtgccgcc ggcaaaggct
     8161 catagcgcga tttccaaaat gaaaacggct gaatattctc gttccgattg cgccacgcct
     8221 cttcgctcaa atcttcgcgt cgataaccat cggcgtcgtg aaaaagcagg gtgtaaaaat
     8281 attctccggg cgcgaacggt cgatgggtcg cggcacaggc atccgcccgg tgcttgatcg
     8341 cccattcgtc tgacagcgga ttcattgcgc aactatacgt cgcgcggtga aaagatgaaa
     8401 gccaggcgct tcgcggttgg aagcgcgaag atagaacaga tagaggccaa tcgcagtgaa
     8461 aaggagattc ggcgtccagg ccgcgatcca gggagagacg cggtcgcctt cgccgagcgc
     8521 gagaaaaagc tggaccagga agttcatcga gaaaaccaga actaccgcag cggcaacact
     8581 tgatagcacg ccccggcgcg agtacccaat tccgagcggg gccgcgatac aaacgaccac
     8641 cagacacgtc cacggcaggg cgagacgata ttggagatga gtacggaacg gcgcgagcag
     8701 agtaggggga aaatccgaat tgaaatgcag atactcccgt agctccggca agcttaggaa
     8761 ttctgcgcgc acgtttgcgc tacccaaccg gaaaggggtc tcgctccaat gctcgacctt
     8821 gagcgaagga taaatctctt cgtcgacgat atttcccgca tgatcgtaat gaacgacttt
     8881 cgcattctcc agatcccagg tttttgtttc gggacgatag aaagcgcgcg ttgcgacgta
     8941 attcgtcacg atattgtcgt tcgcgtcctg ttggagcacc tggacgttgt tgaacgcgtt
     9001 gccgcccagg cgaaaatttt gaataaacca ggtgcgcgcg tcggttcggt tccggaaaat
     9061 ttgtccctcg atagaggctt ccggacggct acgcgcttgt gagaggagcg ctttgcgcgc
     9121 cagttccgca tgcggcgcaa gcgagtagtt tagcgccatg ctgactccca ccgtgagcag
     9181 cccgatgccc atcaatggaa ggagaacgcg cggtaaactt atgccggctg tcagcatgga
     9241 tacgatctcg ttcgcccgcg acattcgccc cagggtgaaa aggagcgcga gcaagagaga
     9301 aaccggcagg agaatgatga aaatctgtgg gacctgggtg gcgtaatact gggcaacgac
     9361 aggaaggccg atgtgttgct cgataaaggt cgagatgttg tcgctgatat cgaagatcaa
     9421 ccagatcgag ataaaaccag cgatgcagta gagatagacc tgcaagaaat tgcgaacgac
     9481 gtaacgatcg aggagtctca cgagattttc gaatttcgat ttgcgattgg cgaattacta
     9541 tagcatctcc gccggcggtc ggaacacagg catcctgcct gtgcggcgcg cgggcttccc
     9601 agcctgcgcg gatccattat ccaagaaaac aatttctacc atctctttcg ggacttcaaa
     9661 aaaccattat gatcacccag cgttaaaacg ctggcctatt tttttcgcaa tgatcgcgct
     9721 aaaagattct ctaggcaccg aatttgtcga aagcgttcgc gcattttttt ccgagaacgg
     9781 gccactctcc aaggcgaaga atttcgagtt ccgtttgcaa cagcaggaaa tggcggcagc
     9841 cgtggcgaaa gcgctggagg aagaacgcca tctggtggtt gaagcgggca ccggtgtggg
     9901 caaatcgctg gcctatctca ccccggcaat tctctttgcg cttgagcagc acaaaaaggc
     9961 gatcgtctcg acccacacaa ttaatcttca ggagcagctt ctttataaag atatcccgat
    10021 cttaaagaag attttgccgg tcgagttcga cgctgccctc atgaaagggc ggcaaaatta
    10081 tctttgcccg cggcgattgg aacgcgcgct ccagcagcag aacgagcttt ttactggccc
    10141 ggaacaaaac gagttaacga gacttgcgga atgggcgcgc acgactcgcg acggctcgct
    10201 gagcgactta ccgatcgagc ccgatccaaa agtttgggtg caagtttgca gcgaaccaca
    10261 catctgcacg gtgaaaacgt gcggccaggg ccctggctgt ttttatcagc aagcccgcaa
    10321 gcggcttctt tcctcggacg tgattgtgat caatcacacg ctgctcttca tgcttttggg
    10381 aagcccagat cagcaggaag aacgcgagag cggctatctt ttcccaaacg acttcatcat
    10441 tttcgatgag gcgcacacag tcgagcgagt cgcttcccga cagatcggga tcggcatttc
    10501 gcaatacggg ctaagatcga cagtccagcg cctctacaat gcccgaacgc gcaaggggct
    10561 gttcacggtt atgcgcgacg caatcggggt gcgattggcg gcggaagctg tcgatgagct
    10621 ggacaagttc ttcggcgccg ttgaatcgcg ctcggacttt cgaaagggcc gggaatttcg
    10681 ggtgcgcgcg ccggagcttg ttcccgacac gattactgga cgcctgactg ggttgcaggc
    10741 gcgtatcgcc gaagtcgtca ggcgcgccga tgacgaattt ctaaaagccg aattacagga
    10801 gctgggccgg cgtattggcg atgcccgcga cggcatcgcc atctttctgg aacaaagcgc
    10861 tcgcgaacac gtttactggg tcgagcgcac gggaaagaca gcgcaattcc tctcactaaa
    10921 tgcggcgcca atcgacatgg cgccggtgct gcgccgcatg atttttcggg aaggctgctc
    10981 ctgcgttatg acgagcgcaa cgctctcagt tggccgtcct gacctcgcct atttccggcg
    11041 gcgcatcggg gcagacgaag tcgaaccgtt gttgctcgga agtcccttcg atttccggac
    11101 gcagatgaaa ctgttcattg tgcagaaaat gcccgatccc cgcgatgcga cttacgagaa
    11161 agcgctcgag cattggatcg cgcattttat cgaggaaacg gacggacgcg cctttgttct
    11221 ttttacgagt tatcgcggca tgcagcaggt ggccgatcaa atgggggaat tcttttcgcg
    11281 aaagaaattc aatcttctgg tgcaaggcgg aggcgcgcca cggggccgtt tgctggagca
    11341 attcaaaacc accccacgca gcgtcctctt tggcaccgac agcttctgga tgggtgtcga
    11401 tgttccgggc gaggcacttt cgaatgtgat catcacccgg ctgccatttg cggtcccgga
    11461 ccatccgctg atcgaagcga aactggagct tgtggaagag cgcggaggcg atccgttcac
    11521 cgaatattcg ttgccagaag ctgtcctcaa acttcgacaa ggcgtcggcc gcttgattcg
    11581 gacaaaaacc gaccgcggca tcatcgtcat tctggacaat cggatcgtga gcaagccgta
    11641 cggacgcgcc tttctgcaag cgttgcccaa gtgcccggtg aaaataattt gaaatgttcg
    11701 cttgaacata tattgcgata aagcttaaat tgagcgcatg aagcgctctt caggatcgaa
    11761 ttccgcggcg gcgaaaaatc gagaagctga ggttcggcaa atcctggcga ttttgcacaa
    11821 gtgggggatt catacccttg gccaattcgc tgcccttgat aaggagcagg ttaacgcccg
    11881 gctcggtctt gaagcggttc gaatatggga acgcgcgaac gggcaatccg atcggccgct
    11941 caaactcgtt cgaccgcctg agtcgttcga ggagagcttt gaatttgaga acgaaattga
    12001 aactgccgag ccgctcctct ttatgctgcg tcgttttttg gagcaactcg cggtgcgctt
    12061 gagcgcgatt tatctcgtcg caaaggaact gacactgcga attatatttg gaaataaaca
    12121 aacgtacgaa cgccttttca aaattccgca gccgaccaat gatgtcgatc ttctctttcg
    12181 gatgctgcaa acgcacctgg aaaattttaa atccgagcat ccgattgttg ctgtcgcgct
    12241 gagtgcgcag ccgattaaac ccgagaaaga acaattcggc ttatttgaaa ccacattact
    12301 caatccacag caactatcgg aaacacttgc gcgcttgacc gctctgcttg gcgctgatcg
    12361 agttggcacg ccggttttgg acgaaacata ccggccggat gcatttcaca tggagccgtt
    12421 ttgtagcaca ggcttccagc ctgtggggca agcaggggtt ctcgcctgcg agagaaccgg
    12481 caggcaagat gccccccggc cccacagcca ggatggctgt gccacgtcga aggcatcgct
    12541 gcgccgattt cgaccagctc catctgcatc cgtttttctg ttcgaaaata ttccggctca
    12601 tattcgaagt gcagatatcc gcggcaaagt tgtgcagcag cgcggcccgt atttaagttc
    12661 aggaaattgg tgggacgaaa aatcgtgggc gcgcgccgag tgggacctcc agatcgagga
    12721 tggtgaactc gttcggtgtc acgaatctcc gccttcatcg aattacggcg cgatagggga
    12781 gggaacttgg gaaatggatg ggctttatga ttaacgatcc ggagtcatcc tggaatgacg
    12841 atgagctaca tcgagttaca tgcgtgcagc gcatttagct ttctccgcgc cgcttcgttt
    12901 ccggagcaat tagccgaagt cggggctgaa ttggaaatgc cggcgttggc gttgctcgat
    12961 cggaacggcg tttacggggc gcaacgcttt tcggtcgcag cgcgcgagca cggcgtgcga
    13021 ccaatcatcg gcgcggagct atcgatggaa gatggcggta ttctgcccgt gctggtcgaa
    13081 aaccgcacag gttacaaaaa tctttgcgag ctgctcacgc aggcgcattt gcggagtgag
    13141 aaagggaact gcgcgattca gtggaatgag ctgccgcaat ttgcggaag
//
LOCUS       DASQUQ010000121         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_389006, whole genome shotgun sequence.
ACCESSION   DASQUQ010000121 DASQUQ010000000
VERSION     DASQUQ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389006"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VE486_03475"
     CDS             complement(<1..966)
                     /locus_tag="VE486_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZA38178.1"
                     /translation="MANETSSANPSLPLHEVLEAEFTTLHGELPADYPTSTEPEVRLK
                     AIWATVHALKEKRAALCLSGGGIRSATFGLGVLQGLARCGLLDRFQYLSTVSGGGYIG
                     GWLSAWIHRAEGGLRSVATQLARPRDNSRPNPEPVEIQNLRSYSNYLSPRLGLFSADA
                     WTLFATYIRNLLLTSLVMIPLLAAALTVPWFYEAVLMKNPIPYTSIPLWIGVAGVAMG
                     VTYMGINLPCGGNKRGSQRSFLIFCLLPLLIAAFSMTTYWAWFVAYGRTTPKWSFLGL
                     TPPHQVVPFIYLGVLMHLISWVTSLLRAHGFRLKEFIAVIASGAIG"
     gene            1299..2120
                     /gene="dapF"
                     /locus_tag="VE486_03480"
     CDS             1299..2120
                     /gene="dapF"
                     /locus_tag="VE486_03480"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZA38179.1"
                     /translation="MLDETRMLRFTKMNGAGNDFVLIDNRAGEVRLDRSQIANLCDRH
                     RGIGADGVLLLEKPANRADFRMRYFNADGGEAEMCGNGARCFARFANKVAGAAGKISF
                     ETPAGVISAELIDDLVTLQMTEPTDLRLSVPLRIANQYEAIHFINTGVPHVVIPVPRI
                     DDIDVEREGAAIRQHEMFSPNGANVNFIEKRGANKIAVRTYERGVEDETLACGTGVVA
                     SALIFAATEDVNGPIGVFVRGGNELQVGFEKTGTEFKNVTLTGPAEFVFEGTIEV"
BASE COUNT          575 a          659 c          667 g          481 t
ORIGIN      
        1 cccgatcgcg cctgatgcga tcacggcgat gaactccttt aaccgaaacc cgtgcgcacg
       61 aagtagcgac gtcacccacg agataagatg catcagcacg cccagataga taaacggcac
      121 gacctgatgc ggaggagtga gtccaagaaa cgaccacttg ggcgtcgttc ggccataggc
      181 cacgaaccag gcccagtacg tcgtcatcga aaaagccgcg atcagcagcg gcaatagaca
      241 aaaaatcaaa aaactgcgct gactgccgcg cttgtttccg ccgcacggca aattaattcc
      301 catgtaggta acacccattg cgacgccggc cacgccgatc caaagaggga tcgacgtgta
      361 cggaattgga tttttcataa gcactgcttc gtaaaaccac ggaacggtca acgccgccgc
      421 cagcagcgga atcattacca gcgaagtcaa aagcagattg cgaatgtaag tggcgaacaa
      481 cgtccaggcg tcggcggaaa agaggcccag acgcgggctc aggtaattgc tgtagctgcg
      541 cagattctga atctcgaccg gctccggatt cggtctcgag ttgtcgcgtg gccgcgccaa
      601 ttgcgtcgcg acgcttctta ggccgccctc ggcccgatga atccaggcgc tcagccagcc
      661 gccgatatat ccgcccccgg aaacggtgga gagatactgg aacctgtcga ggaggccgca
      721 gcgtgccaac ccttgtaaaa ccccgagtcc aaacgttgcg ctgcgaatgc cgccgccgga
      781 caggcagagc gctgcccgct tttccttcaa cgcatgaaca gtcgcccaaa ttgcctttaa
      841 tcggacttcc ggttcggtcg aagtcggata gtctgcaggc agttctccgt gcaaagtcgt
      901 gaactcggcc tccagcactt catgcaatgg aagagaggga ttggccgatg aagtctcgtt
      961 cgccatgctg cgaaaatgcg gcggtccgca cgcgagcgtt tatcaacatg cggccgaagc
     1021 gtcaatcttg atcgagcgcg acgctctttc agacggtgct ttttcgcccg acagttatct
     1081 ggctggcgag agcaatttat ttgtctcgcc agcgtcaccg ctatttatcg gcaaattttc
     1141 caccgaacca cttcatgcga agggctggag caacctctta ctgttaatca tttacagatg
     1201 gtcccgatgc agcttgtcct gagcggagtc gaggggctcg acatgacaac tgaaaaagag
     1261 cgcgccacga cagcgccggt tggctttcgc tgcagactat gctagacgaa actcggatgc
     1321 tgcgtttcac caaaatgaac ggcgcgggaa acgatttcgt cctgatcgac aatcgcgccg
     1381 gcgaggttcg cctggatcgc agccaaatcg caaatctttg cgatcgccat aggggaatcg
     1441 gcgcagatgg cgttctgttg ctcgaaaaac cggcaaaccg cgcggatttt cggatgcgct
     1501 attttaatgc cgatggcggc gaagcggaga tgtgcggcaa cggggcgcgc tgcttcgccc
     1561 gttttgcaaa caaggttgct ggggcggctg gcaaaatttc ctttgaaact ccggcgggcg
     1621 tcatttcggc tgagctgatc gatgatctcg taacgctgca aatgactgag ccgactgatt
     1681 tacgattaag cgtcccgcta cgcatcgcta atcaatatga agccattcat ttcatcaaca
     1741 ccggcgtgcc gcacgtagta atcccggtcc cgcgaatcga cgacattgat gtcgagagag
     1801 aaggcgcagc catccggcag cacgaaatgt tttcaccaaa tggcgcgaac gtaaatttca
     1861 ttgaaaagcg cggagcgaac aaaattgcgg tccgaactta tgagcgcggc gtggaagatg
     1921 aaacactcgc gtgcggcacc ggcgtggtcg cgagcgcact catcttcgcc gcgaccgagg
     1981 acgtaaatgg cccaatcggc gtattcgtcc gcgggggaaa tgagcttcag gtgggttttg
     2041 aaaaaacggg aacagaattc aagaacgtga ccctgaccgg tccagcggaa tttgttttcg
     2101 aaggaacgat cgaagtctga gttgaagcat ggagtagtgg agtgatggag tattgttgga
     2161 atcagttcat tactccgttg ctccaacact ccatcactcc atcctgaaaa caatgtttcg
     2221 cggcacattc actgcgcttg tcaccccgtt tcgcgatggt gcaatcgata gttcggcttt
     2281 tgcgaaactg atcgaatcgc aaatcgccgc cgggattacc ggaatcgtcg cagtcgggac
     2341 gaccggcgaa tcgccgacgc tttcgcacga ggaacgcgaa ca
//
LOCUS       DASQUQ010000122         7625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1305754, whole genome shotgun sequence.
ACCESSION   DASQUQ010000122 DASQUQ010000000
VERSION     DASQUQ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305754"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /gene="trpA"
                     /locus_tag="VE486_03485"
     CDS             complement(<1..306)
                     /gene="trpA"
                     /locus_tag="VE486_03485"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZA38180.1"
                     /translation="MSRIRETFVELKRSGRCGFIPFIVAGDPDLATTQLLLMELATAG
                     ADIVELGVPFSDPVADGEVIQRASERALRNRVTLRDALTCVARAKQHINVPIVLFSYF
                     "
     gene            complement(303..1526)
                     /gene="trpB"
                     /locus_tag="VE486_03490"
     CDS             complement(303..1526)
                     /gene="trpB"
                     /locus_tag="VE486_03490"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZA38181.1"
                     /translation="MQKPPITRSEPDEHGHWGKFGGRYVPETLVAPLEELTKEFTRAR
                     DDAGFWHELDQFLRNYCGRPTPLFHARRLTDHAGGGQIYLKREDLLHTGAHKINNAMG
                     QALLARRMGKHRIIAETGAGQHGVATATVCALFGLRCIVYMGAEDVRRQALNVFRIRL
                     LGAEVVTVNAGTRTLKDAINEALRDWVTNVHDTYYLLGSALGPHPYPLMVREFQRVIG
                     REARKQILSATGKLPRALIACAGGGSNSIGLFHPFVADEEVRLIGVEAGGRGDLSGEH
                     AARFHSGAASGGGRVGVLQGTMSYVLQDPNGQIATTHSISAGLDYSAIGPEHAFLHDN
                     GRAEYIRATDAEALEGFRLCARLEGIIPALESSHAILPAIRIAHELPQDDAIILNLSG
                     RGDKDVQLVAELQTQ"
     gene            complement(1554..2186)
                     /locus_tag="VE486_03495"
     CDS             complement(1554..2186)
                     /locus_tag="VE486_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZA38182.1"
                     /translation="MTPVQIKICGVTTVKDARACGELGAHMIGLNFYPESPRFIELER
                     ARQILEAIPRGVQAVGVFVDSTAEEVRNVASTAGVQCVQLHGDFSPESCAELAREFRV
                     IRALCTDALFQPEQASLFPECDVLVDAHHPNLRGGTGLTCDWPAARATLSFARFVILS
                     GGLNVQNVAQAIATVTPHAVDVCSGVETAPGEKDYRAVENFIAAVRMTGH"
     gene            complement(2183..3019)
                     /gene="trpC"
                     /locus_tag="VE486_03500"
     CDS             complement(2183..3019)
                     /gene="trpC"
                     /locus_tag="VE486_03500"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZA38183.1"
                     /translation="MSTHFLSEILAQKRKLMAPRPSEASGFRERALELRRNVARHRLL
                     QALESESARLKIIAEFKRRSPSSGIIRDDLAADEVARRYERGGACAISVLTDEQYFGG
                     SIADIGAVRSSTDLPVLRKDFIIDPVQIYEAAIVGADAVLLIVAVLDDASLGRLRGLA
                     EDDLGLDALVEVHTSDELRRAVRAGAKIIGVNNRDLKTFRVSLNTSERLMAEAPADVI
                     MISESGLHGAEPLRHLRALGFRGFLIGQALMRASDPETALRDLIAAAEDRQVARTGAR
                     RS"
     gene            complement(3016..4158)
                     /gene="trpD"
                     /locus_tag="VE486_03505"
     CDS             complement(3016..4158)
                     /gene="trpD"
                     /locus_tag="VE486_03505"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZA38184.1"
                     /translation="MNPKENKRGVAQRGRLLPTPRRTDEIGREAATGIGDFELRQTTL
                     RLMRGENLGRAEAENFLGALLSPAATDAQVAAALALLAVKGETVEELAGMAEAMRNRA
                     ISVRCHHKRFIDTAGTGSSAAKTFNVSTAAAFVIAGAGLPVAKHGSRAATSYSGSADV
                     LEALGVNTAAPPAIVERCLNEHGICFMFAPLFHGATARVAHVRRELGVHTTFNLLGPL
                     TNPARAPFQILGVWHSALLEPVAAALSLLGTEKAWVVHGADGLDEITMTGQTFVAACS
                     KGSSVEKFTISPEGFGLKRRSSDGIPGAGPIENAQLVRAILKGKAEGSFASARDLVII
                     NAGAALHIAGLAPNLREAASLARESIDSGRAGSKLEALITETNRKE"
     gene            complement(4180..5670)
                     /gene="trpE"
                     /locus_tag="VE486_03510"
     CDS             complement(4180..5670)
                     /gene="trpE"
                     /locus_tag="VE486_03510"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZA38185.1"
                     /translation="MKPQITPTLDEFSQLAKHGNIIPVFAEFVADGETPVSAFKKLDH
                     GGYSFLFESTEKNDVSGRFSFAGIGPRAVIQSYGREIRIAEREKEWRFETATDPLDEV
                     RKLMARYQFVSRPDLPRFAGGAVGFLGYEGIRFFEPKVPLAHHDHLRLPETLFMLTNS
                     VLIFDHQLRSLKIAVSAFLDDGPLEAVYAQAAESIDAIVRQLGKSAHLPLIPTQDRQA
                     APARSNFRPDEFERAVERAMEYIRAGDIFQVVLSQRFESNFAGDPLDFYRCLRFINPS
                     PYMFCLKFCDEFALVGSSPEMHVRLTGDTVEIRPIAGTRPRGITPAQDERNAAELLAD
                     PKERAEHIMLVDLARNDVGRIADFGSVRVTEMMEIERYSHVMHIVSNIAGRLRAGCNA
                     FDLVKATFPAGTVSGAPKIRAMQIISDLEQTRRGCYAGTIGYFGFDGNFDSCIALRCA
                     VLKDGTAYFQAGAGIVADSDPRLEYAETVNKARAMAKALAMATEME"
     gene            complement(5671..6243)
                     /locus_tag="VE486_03515"
     CDS             complement(5671..6243)
                     /locus_tag="VE486_03515"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZA38186.1"
                     /translation="MLLLIDNYDSFTYNLAQYFGELGSEMLIKRNDEIALDEIAELRP
                     DHICISPGPCTPREAGISKEVILRFAPDIPILGVCLGHQCIAEAYGGKVIRASRLMHG
                     KASAVRHNGSALFSDLATPFEAGRYHSLSVERSSFPTSLEIMAESDDGEIMALRHREF
                     PVQGVQFHPESILTRDGKKILANFLSCPGH"
     gene            complement(6259..7260)
                     /gene="aroF"
                     /locus_tag="VE486_03520"
     CDS             complement(6259..7260)
                     /gene="aroF"
                     /locus_tag="VE486_03520"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZA38187.1"
                     /translation="MKSSATAHEIDAVVEIIQAIGLRAHPMPGATRTAIGITGNEGPI
                     DGRRFENLLGVAEVIRVTKPYKLITLDLRPEKTIVRVGDATIGGSELAIIAGPCAIEN
                     RDQAFAVAEAVQHSGARFFRGGVWKPRTSPYTFQGLGDKAWEIMADIRQTFGLKIVTE
                     AVDESNIDLIEQYGDIIQIGARSMQNFSLLKKAGRSKLPVLLKRGMAATLEEWLLAAE
                     YIMAEGNYNVILCERGVRTFTQHTRNTLDLASVPAIRRISHLPVIVDPSHGTGTAYMV
                     TPLARAGVAVGADGLMVEVHNHPELALSDSAQALTPSEYAQLIKEVRAIHKLTSSPS"
BASE COUNT         1619 a         2194 c         2157 g         1655 t
ORIGIN      
        1 gaaatagctg aaaaggacaa ttggcacgtt gatatgctgc tttgcacgcg cgacgcatgt
       61 cagcgcgtcg cggagggtaa cccggttgcg caaggcgcgt tcagatgcgc gttgtatcac
      121 ttcgccatcg gcgactggat cgctgaaagg gacgccgagc tcgacgatgt ctgctccggc
      181 ggtcgcaagt tccattagca gaagttgcgt ggttgccaga tcgggatcgc ccgccacgat
      241 gaacgggata aagccgcagc gtccggagcg ctttagctcg acgaacgtct cccggattcg
      301 gctcattgcg tttgtaattc cgcaacgagc tggacgtctt tatcgccgcg gccagacaaa
      361 ttcaggataa ttgcgtcatc ttgcggcagt tcatgcgcta tacgaatagc tggcaggatg
      421 gcgtgcgagc tttcgagcgc gggaatgatg ccttccagtc tggcgcaaag ccggaatcct
      481 tcgagcgctt cggcgtcagt tgcccggatg tattcggctc gtccgttatc atggagaaac
      541 gcgtgctctg gaccgatggc agagtaatca agcccagccg agatcgaatg cgtcgtcgca
      601 atctggccgt tcggatcctg aagcacatac gacatcgtgc cttgcaacac accgacccgt
      661 ccgccgccgc tcgccgcgcc actatgaaat ctggcggcat gttcccctga caaatcgccg
      721 cggcccccgg cttccacgcc gatcaatcgg acctcttcgt ctgcaacgaa aggatgaaac
      781 aaaccaatcg agtttgagcc gcccccggcg cacgcaatca gcgcgcgcgg taattttcca
      841 gtcgcggaca aaatctgttt gcgcgcttcg cgtccaatca cacgctgaaa ctcgcggacc
      901 atcagcgggt atggatgcgg accgagcgcg cttccgagca gatagtaggt gtcatgcaca
      961 tttgtcaccc agtcacgcag cgcctcatta atcgcatcct ttagcgtgcg cgtgccggca
     1021 ttgacggtca cgacttcggc gccgagcaat cgaattcgaa aaacgttgag cgcctgacgc
     1081 cgcacgtctt ctgcgcccat ataaacgatg cagcgcaatc caaagagcgc gcagactgtt
     1141 gccgtcgcaa ccccgtgctg gcctgcgcct gtctcggcga tgatgcgatg cttgcccatt
     1201 cgccgtgcga gcaaggcttg tcccatggca ttgttgatct tgtgcgcgcc ggtgtgcaaa
     1261 agatcttcgc gctttagata aatttgccct ccgcccgcat ggtcggtcag tcgcctggcg
     1321 tgaaacaacg gcgtcggccg gccgcaataa ttgcgcagga actggtcaag ttcgtgccag
     1381 aagcccgcat cgtcgcgcgc gcgcgtgaac tctttcgtca gctcttcaag tggcgccacc
     1441 aatgtttctg gaacatagcg gccgccaaac ttgccccaat gtccgtgctc gtcgggctcg
     1501 ctgcgtgtga tcggtggttt ttgcacgcga aattaaggcg agggtgcgct cagtcagtgc
     1561 ccggtcattc gtacggcagc aatgaaattt tcaacggcgc gataatcttt ttcgcccggc
     1621 gcagtctcaa caccgctgca gacgtcaacc gcgtgcgggg tgacggttgc gatggcctga
     1681 gcgacgttct gcacattcag accacccgat agaataacga atcgcgcgaa tgatagcgtt
     1741 gctcgcgcgg ccggccagtc acatgtcagg ccggtgccgc ctcggagatt cgggtgatgc
     1801 gcgtcaacca gcacatcaca ttcgggaaat aaagaagcct gttcgggctg gaacaaggca
     1861 tcggtgcaaa gagcccgaat gacccgaaat tcgcgagcca attcagcgca cgactcaggc
     1921 gaaaaatcgc cgtggagttg aacgcattga accccggctg tgcttgcgac gtttcgcact
     1981 tcctccgcgg ttgagtcaac gaaaacgcca actgcttgaa cgccgcgcgg tatcgcttca
     2041 aggatttgcc gggcgcgttc gagttcaatg aagcgcgggc tttcaggata aaagttcaga
     2101 ccaatcatgt gggcgcctag ttcaccgcaa gcccttgcat ctttcacggt ggtgacgccg
     2161 caaatcttga tctgcactgg agtcatgatc gccgcgctcc agttcgcgca acttgtcgat
     2221 cttcggcggc tgctattaaa tcacgaagag ctgtttcagg atcgctcgcc cgcatcagcg
     2281 cttggccgat aagaaaaccg cggaatccaa gtgcgcgaag gtgacgcaat ggttccgcgc
     2341 catgcaatcc gctttcgcta atcataataa catctgccgg tgcttcggcc atcagacgct
     2401 cgctagtgtt cagtgaaacc cgaaacgtct tcagatcgcg attattcact ccgatgattt
     2461 tcgcgcctgc tcgcaccgcg cggcgcagtt cgtcggatgt gtgcacttca acaagtgcat
     2521 cgagtccaag atcgtcttcg gccaaacctc gcagacgccc taacgaagcg tcatccagta
     2581 ctgccacgat tagaaggacg gcgtcggcac ccacaattgc ggcttcgtaa atctggaccg
     2641 gatcaatgat gaaatctttt cgcagaaccg gcaggtccgt gctggagcga accgcgccga
     2701 tgtccgcaat cgaaccaccg aaatattgct cgtcggtcag aacggaaatc gcgcaagcgc
     2761 cgccgcgctc atagcggcga gcaacctcat cggccgcaag gtcgtcgcga ataattccag
     2821 acgacggtga tctgcgtttg aattctgcga taattttcag tcgcgctgat tccgattcca
     2881 gtgcttgaag cagacgatgc cgtgcgacat ttctccggag ttccagcgcg cgttcgcgaa
     2941 atccagaagc ttcacttggc cgcggtgcca ttaatttccg cttctgcgcg agaatctccg
     3001 agagaaaatg tgtgctcatt cctttcgatt tgtttcggtg atgagcgcct ccagcttcga
     3061 ccccgcacgt ccgctgtcga tgctttcgcg cgcaaggctc gccgcttcgc gaaggttggg
     3121 tgcaagcccg gcaatgtgta gcgctgcgcc agcgtttata atcacgaggt cgcgtgcgct
     3181 agcgaagctt ccctctgctt tacccttcag aattgctcga actagctgcg cattctcaat
     3241 tggcccggcg ccgggaattc catcggacga tcggcgtttc agtccgaaac cttcaggaga
     3301 gattgtaaac ttttccacgc tggagccttt cgagcaggcg gcaacaaaag tttggccggt
     3361 cattgttatt tcatcgagac cgtcggcgcc atgcacaacc catgcctttt ctgtgccaag
     3421 cagagagaga gcagccgcga ctggctcgag taacgcagaa tgccagacgc caagaatctg
     3481 aaagggggcg cgggcgggat tcgtcagggg accgagcagg ttgaaggttg tatgcacgcc
     3541 gagttcacgg cgaacatgcg ccacgcgcgc tgtggcgcca tggaataggg gcgcaaacat
     3601 aaaacagatt ccatgctcgt tgaggcagcg ctcgactatt gccggcggag cagccgtgtt
     3661 tacgccgagc gcttccaata cgtcggcgct cccggaatac gatgtcgccg cacgcgaccc
     3721 atgtttcgcc accggcagac cggcgccggc gatgacgaaa gctgccgctg tcgagacgtt
     3781 gaaagttttt gccgcgctcg agcccgtgcc tgcggtgtca atgaagcgct tgtggtggca
     3841 ccgcactgaa atggcgcgat ttcgcatggc ttccgccatg ccggccaatt cttcgaccgt
     3901 ttcgccttta acggctaaca aagcaagcgc cgccgctacc tgcgcgtcgg ttgcggcagg
     3961 actcagcaac gccccgagaa agttttcggc ttcagcacgg ccgagattct cgccgcgcat
     4021 gagacgcaat gtcgtttggc gcagttcgaa atcgccaatg ccagtcgccg cctcgcgtcc
     4081 aatttcatcc gttcgccgtg gcgttggcag caaccgacca cgttgagcaa cgccacgctt
     4141 attttccttc ggattcattt gcgtaatcgc atcggcctct cattccatct ccgtcgccat
     4201 tgcaagagct ttcgccatcg cgcgcgcttt gttgaccgtc tcggcatatt ccaggcgtgg
     4261 gtccgagtcg gcgacaatgc cggcgccggc ttgaaagtac gctgtaccgt cttttagcac
     4321 ggcacagcgc agcgcgatgc aggaatcgaa attaccgtcg aaaccaaaat agccgatcgt
     4381 gccagcgtaa cagccgcgcc tggtttgttc caggtcgctg atgatctgca tcgcgcggat
     4441 tttcggcgcg cccgagacgg tgccggcagg aaatgttgct ttgaccaggt caaacgcgtt
     4501 gcaaccggcg cgcaaacggc cagctatatt ggaaacgata tgcatcacgt gactgtaacg
     4561 ctcgatctcc atcatctcgg tcacgcgcac tgaaccgaaa tccgcgatcc gccccacatc
     4621 attgcgggcg agatcgacca gcatgatgtg ttcggcacgt tccttgggat cggcgagcag
     4681 ctcggcggcg ttgcgctcat cttgcgcagg agtgataccg cgcggacgcg tccctgcaat
     4741 cgggcgaatc tcaactgtgt cgccggtcag ccggacatgc atctccggcg agctgccaac
     4801 cagcgcaaac tcgtcgcaga atttaagaca aaacatgtac ggggacggat tgatgaaacg
     4861 gagacaccga taaaaatcga gcggatcacc ggcaaaattg gattcgaatc gttgcgagag
     4921 gacaacctgg aagatgtcgc cggcgcgaat gtattccatg gcgcgttcga ccgcgcgctc
     4981 aaattcgtcg ggccggaaat tgctgcgagc gggcgccgct tgacggtcct gagttggaat
     5041 aagcggtaga tgagcagatt tccccagttg gcgcacgatg gcgtcgattg attctgccgc
     5101 ttgcgcgtac actgcttcca gcgggccgtc gtcgagaaat gcgctcacgg cgatcttcaa
     5161 gctgcgtagc tgatggtcaa aaatgagaac gctgttggta agcatgaaca gcgtctcggg
     5221 caggcgcaga tgatcatgat gtgccaacgg aacttttggt tcgaagaagc ggatcccttc
     5281 gtacccgaga aagccgacgg cgccacccgc gaaccgcggt aagtccgggc gtgagacgaa
     5341 ttgatagcgc gccattagtt tgcggacttc atcgagcggg tcggttgccg tttcgaagcg
     5401 ccattctttc tcacgttcgg caatccggat ttcgcgtccg tagctttgaa tcaccgcccg
     5461 cgggccgatg ccggcgaacg agaaccgtcc cgacacatca ttcttttcgg tcgattcaaa
     5521 taggaaactg taaccgccgt gatcaagttt cttgaaggct gaaaccggcg tttcaccgtc
     5581 agcgacgaac tctgcgaaaa cagggatgat gttcccgtgt ttcgcaagct gagaaaattc
     5641 gtccagcgtc ggagtgatct gcggtttcat ttaatgtccc ggacatgaaa ggaagttcgc
     5701 gagaattttt ttgccgtctc gcgtaagaat tgattcggga tggaattgca ctccctgcac
     5761 cgggaattcg cgatgtcgca gcgccatgat ctcgccatcg tcgctctcgg ccatgatctc
     5821 gagagatgtt gggaaggaac ttcgctcgac ggagagggaa tgatatcggc cggcttcgaa
     5881 cggtgtcgcc aaatcggaga agagcgcgct tccgttgtgc ctgaccgccg atgcttttcc
     5941 atgcataagc cgggaagcgc ggattacttt tccgccgtat gcttcggcga tgcactgatg
     6001 accgagacaa acgcccagga tgggaatgtc gggcgcgaag cgcagaatta cctctttgct
     6061 gatgcccgct tcgcgcggcg tgcatgggcc gggcgaaatg cagatgtgat ccggtcttag
     6121 ctccgcaatt tcatccagcg cgatctcatc gtttcgttta atgagcattt cagacccgag
     6181 ctcgccgaaa tactgcgcga ggttgtaggt gaaggagtcg tagttatcga tcaggagcag
     6241 catggctttg gaaagtagtc aggaaggcga tgacgtaagc ttgtggatcg cgcgaacttc
     6301 cttgatcagc tgcgcatatt cggagggtgt cagcgcttgc gcgctatcag aaagagcaag
     6361 ttccggatgg ttgtggactt cgaccatgag accatccgct cccactgcta cgccggcgcg
     6421 agcgagtggt gtgaccatgt aggcagtacc cgtgccgtgg gatggatcta cgattacggg
     6481 caaatgggaa attcgacgaa tcgctggtac agaagcgaga tccagcgtat tacgtgtatg
     6541 ctgcgtgaag gtgcgaacac ctcgctcgca taagataacg ttgtagttcc cctcggccat
     6601 gatgtactcg gcggcaagca gccattcctc gagtgttgca gccattcctc tcttcaacag
     6661 gacgggcaac ttcgaacggc ccgccttctt gagaagtgaa aagttctgca tgctgcgagc
     6721 gccaatctgg atgatatcgc catactgctc gataagatcg atattagact cgtccaccgc
     6781 ttccgtgacg atctttagac cgaaggtctg acggatatct gccataattt cccacgcctt
     6841 gtcgcccagt ccttggaatg tgtaaggcga cgttcgaggc ttccacacgc caccgcgaaa
     6901 gaaacgcgcg ccgctgtgct gcactgcctc cgccacagca aacgcctggt cacgattctc
     6961 gatcgcgcac ggtcctgcga taatcgcgag ttcgctcccg ccgatggtag catcgccgac
     7021 gcgcacgatc gttttctccg ggcgcagatc gagcgtgatc agcttgtacg gtttggtaac
     7081 gcgaatgact tctgcgacac cgagcaggtt ttcaaaacgc cggccgtcaa tgggaccttc
     7141 gttacctgtg atgccgattg cggtgcgtgt cgcgccaggc attggatggg cacggagtcc
     7201 gattgcctga ataatttcaa ccaccgcatc gatctcgtgc gcggtggcgc tggatttcat
     7261 cacaaccaac atattgttac aaagccttgg gttatttcat tgacgcgaag actagatctc
     7321 cctatgagag atagaaatca gcgtcccgtt gaataacggt cgctttttat cgccacacta
     7381 ccgacgtcga cgcaaagaga agcggctcgc tatctgtcaa gtctgtgcaa aaaaccatgc
     7441 cagccctcat tacccaaaga ttgtaacctc gatcttgcgg atgtcgatct cagatttcgt
     7501 gccgatgccc ttccgcgtcg tattcgtagg tcttgccgtc ttcctcgtcc ctcgtcgtct
     7561 tgtgtgagcc gtcacaaaac ggctggttcc tggaaaggcc gcacatgcaa atccagacgg
     7621 actct
//
LOCUS       DASQUQ010000123         3913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_389443, whole genome shotgun sequence.
ACCESSION   DASQUQ010000123 DASQUQ010000000
VERSION     DASQUQ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389443"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..321
                     /locus_tag="VE486_03525"
     CDS             148..321
                     /locus_tag="VE486_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38188.1"
                     /translation="MNPAIAHENQADQFTPDDPFYHWVIGLWLQALGMIELADKHFQR
                     AIQLEPGFQACPR"
     gene            494..970
                     /locus_tag="VE486_03530"
     CDS             494..970
                     /locus_tag="VE486_03530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZA38189.1"
                     /translation="MTAVKMPRIRTEVHVMFFDTDCGGVVSNIAYLRFIEIARTLLAE
                     ELGLALAEMAETQKYPVVVHTEIDYRRAAKLGDQLVVEGWLDRLERVRFWCAFRIVRP
                     KDNMLIAECRQSLALIEMPAGMLLRLPESWEKYREAKNDDFLAGEERRRTGDRRSL"
     gene            975..2768
                     /locus_tag="VE486_03535"
     CDS             975..2768
                     /locus_tag="VE486_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZA38190.1"
                     /translation="MTAGARTFTIEETLLEPAALPPPPTRHALFAILIALAALLHVAT
                     IGSGDLYSQTEGQYAGAAKEMVETNNWLLPTNNGIPRLQKPPLLYWLIIVSFKLFGVN
                     AAAARLPVALASVVLVALIFLTGEKLTDYWRGFVAGLIYLSFCGVVLLGRIVMPEPLV
                     SALIAGAIFCGLCGYQRRRYRSAWFAGFWICSALACLTKGFLGIIYPAAIFLLLSIFY
                     REARVRFRGLLRWQYFAMFCLIVAPWHIWAEWHFPGYFSYLVGSEWIGHLRGINDATH
                     DFKGVPVYQFLGIHLAWWFPWSIALLPGLIFAWRRVMRPREIEFSDALPLCWMGVIFV
                     PLLFLGQRQDYYSMSMWSAVALWAAVAWDRMPGRLRAAGAIAIGLIGIASLAVAFPIS
                     NAAHPVDGNWGAMDDRWTAWEAVQNMPASTWLSFRPMFLISGVSLASLSLVALYFVFK
                     QREKLAAVALSVAMIPPGLGMMESVARIAPYFSLADLGRFFNARLDEKGEVLFEGPLN
                     DGSSLIFYLNRKFSLVNQNPKKEAPLGLPSIDIFLDEDALLKKWSRTEAVYLIVEQGR
                     LGHWKPLLTDRFHVYHQVTTSGTYVVLSNQL"
     gene            2894..3049
                     /locus_tag="VE486_03540"
     CDS             2894..3049
                     /locus_tag="VE486_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HZA38191.1"
                     /translation="MSQHRSLKGASTIAAKRNVLKRFERVELLKKRGQWKEGGKVIGL
                     PKTKPDV"
     gene            3148..>3913
                     /locus_tag="VE486_03545"
     CDS             3148..>3913
                     /locus_tag="VE486_03545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZA38192.1"
                     /translation="MRLNRFLAAAGVGSRRYCDELIAAGRVTINGKVCTDFSAQPAPG
                     DHVKVDGKLVHAEPLLTIILHKPAGFVSTRKDVHARDTILDLLPKKFSHLFNIGRLDA
                     QTEGLILLTNDGNLAQRLTHPSYKIDKEYEVTLDRPWDRALASKLFEGIFLDGKRARI
                     HQLHSRGPKRLRVVLRQGINRQIRRMFHEIGYEVKRLVRIRIGNLRLGDLPRGHWRAL
                     TKREVESLRTSSIRGENKRSSAANRQRQQNPPMRRFV"
BASE COUNT          813 a         1086 c         1080 g          934 t
ORIGIN      
        1 tcgaaacgga aattatacat cgtgccccag cggatcgcgt tcgcgttctg attttgggca
       61 aaggtttccg tgctccaggt cagggcgccc cagccgcaga caaaacgccc gaagattaac
      121 gtcgcgagga tcgccagaga aaagaagatg aaccccgcga tcgctcacga aaatcaagcc
      181 gaccagttca ctcccgacga tccgttttac cattgggtga tcggcctctg gctgcaagcc
      241 cttggaatga tcgagcttgc tgacaaacat ttccaacgag cgatccaact cgagcctggg
      301 ttccaggcat gcccgcggta gagcgggact aagcttgccc tgagcgaagt cgatgggggc
      361 gcgcaaaaac acacgcaggc tggaggttct gcgcaacaaa tgtccgtaca atgggaggga
      421 ttcgcacttt cgccaaggat gccaaccaag gattggcatt gctttggaaa gcttttcgga
      481 atgattgttg attgtgaccg ccgtcaagat gccgcgtatc cgcacagagg tgcacgtgat
      541 gtttttcgat accgattgcg gtggcgtcgt gtcgaacatc gcttatctgc gcttcattga
      601 gatagctcgc acactactcg ctgaagaact cggtctggcc cttgcagaaa tggccgagac
      661 gcaaaaatat cccgtggtcg tccacaccga gatcgattac cggcgcgcgg caaaacttgg
      721 ggatcaactg gtcgttgaag gctggctcga tcgattggag cgggtacgat tttggtgcgc
      781 atttcgcatc gttcgcccga aggacaacat gttaattgcc gaatgccgcc aatcactcgc
      841 gctgatcgag atgccggccg gcatgttgct gcggttgccg gagagctggg aaaaatatcg
      901 agaagcaaag aatgacgatt tcctggccgg cgaggaaaga cggcggactg gagaccgccg
      961 ctcgttgtag aataatgacg gcaggcgccc gcaccttcac gattgaagaa acgctcctgg
     1021 agccggcggc gctcccgccg cctcccacgc gccatgctct ctttgctatt ctgattgcgc
     1081 tggcggcgct tttgcacgtg gcgacgattg gttcgggcga cctctacagc cagacggaag
     1141 gccagtacgc cggcgcggcc aaagagatgg tcgaaacgaa taactggctc ctgcccacga
     1201 acaacggtat tccgcgcttg caaaaaccgc cgttgctcta ctggctcatc attgtttcct
     1261 tcaaactttt cggggtcaat gcggccgccg cccgtctccc ggtggcgctc gcgagcgtcg
     1321 tcttggttgc gctgattttc ctgactgggg agaaactcac cgattactgg cgaggcttcg
     1381 tcgccggtct gatttacctc agcttctgcg gcgtcgttct cctcggacgc atcgtgatgc
     1441 ctgagcccct cgtcagtgcg ttaattgcag gcgccatttt ttgtggtctg tgcggttacc
     1501 agcgccggcg ctaccgctcg gcctggtttg caggattctg gatctgttca gcgctcgctt
     1561 gcctgacgaa aggctttctc ggcatcattt atccggctgc gatttttctt ttgctttcaa
     1621 ttttctatcg cgaagcccgc gttcgctttc gcgggctcct gcgctggcag tatttcgcaa
     1681 tgttctgttt gatcgtcgcg ccgtggcaca tttgggcgga atggcatttt ccggggtatt
     1741 ttagttacct ggtcggctcg gaatggatcg ggcacttgcg cgggattaac gatgcgaccc
     1801 atgattttaa aggcgtgcct gtttaccaat ttttggggat tcatttggcc tggtggtttc
     1861 cctggtcgat cgcgctgttg ccgggcctga ttttcgcctg gcggcgcgtg atgcggccac
     1921 gcgaaatcga attttccgat gctctgcccc tctgctggat gggcgtgatc tttgtcccgc
     1981 tcctctttct tggccagcgc caggattact actcgatgag catgtggagc gcggtcgccc
     2041 tgtgggcagc cgttgcctgg gaccggatgc cgggcagatt gcgcgcggct ggtgcgattg
     2101 ccattggttt aatcgggata gcgagcttgg cggttgcgtt tcccatttca aacgcggcgc
     2161 acccggtcga tggaaattgg ggagcaatgg acgaccgatg gacggcctgg gaggcagtgc
     2221 agaatatgcc agcgtccacg tggctttcgt ttcgccccat gttcctgatc agcggcgtct
     2281 ctctcgcctc tctctcgctg gtagcacttt attttgtctt caagcaacgc gagaaacttg
     2341 cggctgtcgc cttgagcgtc gcgatgatcc ccccggggct aggcatgatg gaaagtgtcg
     2401 ctcgaatcgc tccctatttt tctctcgccg acctgggccg ctttttcaac gcgaggttgg
     2461 acgaaaaggg cgaagtgctt tttgaaggac cgcttaacga cggcagcagc ctcatttttt
     2521 atctcaaccg aaaattttcg ctcgtgaatc aaaatccaaa aaaagaagct ccgctcggcc
     2581 tgccgtcgat cgacatcttc ctagatgaag acgcgctttt gaaaaaatgg agccggaccg
     2641 aagctgttta tctgattgtc gagcaagggc gcctcggtca ctggaaaccg ttattaaccg
     2701 accgctttca cgtttatcat caggtcacca cctcgggcac gtacgtcgtc ctgagcaacc
     2761 aattataggg cagcatttct ttggttgagc gttgggcact ctttctcaag ttgaaaaatt
     2821 ccgatcgacg cttaatgtgc cgactcgttt ctcggttttt taactttgta acgctttaac
     2881 ttttaccgaa cccatgtcgc aacatcgcag tttaaaaggg gccagcacca tcgccgccaa
     2941 gcgcaatgtg ctaaagcgct tcgagcgggt cgagcttctc aaaaagcgcg gccagtggaa
     3001 ggaaggcggc aaagtcatcg gccttccgaa aaccaaaccg gacgtgtgat gcgcttggcg
     3061 cgaaaagtga acccttaaga gagttgcaaa gttaaaattc ggcggccgtt ttaacgatgt
     3121 aactttttta accttctaac ctctgctttg cgcctcaacc gcttcctcgc cgctgccggg
     3181 gtcggttcac ggcgatactg tgatgagtta atcgccgcgg gtcgcgtcac catcaacggc
     3241 aaagtctgca ccgatttcag cgcgcagcca gccccgggcg atcacgtcaa agtggacggc
     3301 aaactggttc atgccgagcc actgctaacc atcatattgc acaagccggc aggcttcgtc
     3361 tcaacgcgaa aagatgtaca cgctcgcgac accatcttag atcttctgcc taaaaaattt
     3421 tctcacctct tcaacattgg ccgtctggat gcgcaaaccg aaggcctcat tcttctcacg
     3481 aacgatggca acctcgccca gcggcttact catccgagct acaaaattga taaggaatat
     3541 gaagtgacgc tggatcggcc atgggaccgg gcgctggcct ccaaattatt cgaaggaatt
     3601 tttctcgacg gtaaacgcgc ccgcattcac caacttcatt cacgcggccc gaaacgtttg
     3661 cgcgttgttt tgagacaagg catcaaccgg caaatccgtc gcatgtttca tgagatcggt
     3721 tacgaagtga aacgtctcgt ccgtatccgc atcggcaatc tccgtctcgg tgatttgccg
     3781 cgcggccact ggcgcgcgct gacgaagcgt gaagtggaat cgctgagaac gagcagcatc
     3841 cgtggggaga ataagcgctc ttcagccgca aaccgtcaac gtcagcaaaa tccgccaatg
     3901 cgtagatttg tta
//
LOCUS       DASQUQ010000124         7953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_113528, whole genome shotgun sequence.
ACCESSION   DASQUQ010000124 DASQUQ010000000
VERSION     DASQUQ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113528"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VE486_03550"
     CDS             <1..254
                     /locus_tag="VE486_03550"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZA38193.1"
                     /translation="TMRLFVQTMNFAEGAIGLGVDELRWPNVVRPGDVLTVETEIVDL
                     RPSRSKPNYGIIRLRNVTTNQRGEVVQTMLASAMVPRRG"
     gene            complement(343..1332)
                     /locus_tag="VE486_03555"
     CDS             complement(343..1332)
                     /locus_tag="VE486_03555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZA38194.1"
                     /translation="MKQRKLGRGGPIVSAIGLGCMGMSDFYGGRDEAESLATIDRALE
                     LGITFFDTADMYGPHTNEQLLGRALRGHREQVIIATKFGIVRDPARPEFRGISGKPDY
                     VHQACEGSLRRLGVDVIDLYYQHRVDPDTPIEETVGAMAELVRQGKVRFLGLSEAGAE
                     TIRRAHSVHPITAVQSEYSLWTRDPETEVLGVCRELGIGFVPYSPLGRGFLTGKIQKP
                     EDLPKDDYRHTTPRFQGENFQRNLDLVERVGEIAREKKCTPAQLALAWVLAQGDDIVP
                     IPGTKRRKYLQENAGALNVALTSADLARIEEVAPKEAFAGERYPDWAMQMVNR"
     gene            complement(1474..2409)
                     /locus_tag="VE486_03560"
     CDS             complement(1474..2409)
                     /locus_tag="VE486_03560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZA38195.1"
                     /translation="MRRSSTEKLTRIELSVPREDAKSRLDRFLAKHLPEYSRSRLQHL
                     IRAGFVRLNGVTTRPRQSVRIGDEIELTEPPLEKIANQPEPIPLEILFEDKDLIVINK
                     PAGLVVHPGAGHQQHTLVNALLSHCTTLSGIGGKERPGIVHRLDKETSGCLVVAKNDV
                     THRDLSRQFAERTVEKIYLALVAGKLRKQTGAIEEKIRRHPVYRQRMTVTSLRGRAAK
                     TEYRVVRSGGQASLIECRLHSGRTHQIRVHLHHIGHPVLGDKVYAARQAKDFPRQMLH
                     AWKLGFCHPPSGEWRNFEAPLPDDFATALKLTTES"
     gene            2547..4205
                     /locus_tag="VE486_03565"
     CDS             2547..4205
                     /locus_tag="VE486_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZA38196.1"
                     /translation="MAIEGGAQTCPACGAPVDTSDAEPLAYIACPNCGEKIRVERTFD
                     HFVVVETLGIGGMGAVYKARDTELDRLVALKLLRKDLSGEADHNARLQQEARVAASVS
                     HPNVIQVFDSGTDHGRFYVVMELVDRGSLDDLMERRNQLAEMHVLEIGIQVAKGLRAA
                     HRQGLIHRDVKPANILFVDEYEAKISDFGLAGVAGEESETAAEIWGTPYYVAPERLRK
                     EPEDFRGDIYSLGATLFHAVAGTAPIEGDTNSGAVLLDLKKRPLDLRDIAPEVSEATA
                     SIFQRMIAPDPAQRFSSYNELLSNLEEAQRALIGRTHIPVDHRRRPPRRLRRWLAVLL
                     ILLGATVAMLFALRGRQGNAPARPGPAPPVESDAAEKKIRSVEGDPWNRALANYRQQV
                     ALYNFPQAAAAIKSVKLSDASFKQAKETTEKKAQWLIDWKNELIYDLNRRHFSGAFTG
                     RNGTHYTGIASASDDSLSLKLRYGITRVGWTQLPPKALLKVSTSFIQPTAPDAADRQW
                     RCAVFASETGQPESARGLAEAAAKAKAEYRQQISQLFPDIPQTP"
     gene            4409..6001
                     /locus_tag="VE486_03570"
     CDS             4409..6001
                     /locus_tag="VE486_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZA38197.1"
                     /translation="MEQNPPPFPSPEELKTKITEFMKQNFGDRVSVATFAEPEPAAAV
                     EDEKPEKTEHPEFEFDFLPRDIKAHLDRFVIKQDEAKKVLAIAVCDHYNHANYLRRLE
                     KEDARRAEETEYIKQNVILVGPTGVGKTYLIKHIADLIKVPFVKADATKFSETGYVGG
                     DVEDLVRELVHKADGDVNLAQFGIIYIDEIDKIASAGNLIGRDVSGRGVQTTLLKLME
                     ETEVPVRSMNDLQAQLQAAFEFQRRGKAKRDTINTRHILFVVSGAFGKLKEQVARRVR
                     QGQIGFSASPVQVMDNELFQYVTTQDFIEYGFEPEFIGRLPVRVVCEDLDADDLFKIM
                     KYSEGSLLRQYERAFRAYGIQISFEDEALRLLAEAAAKEKTGARGLLTVFEKLFRDYK
                     YHLAGSGLSQLRVTAGLVREPKRVLDRLMAEGHKLEVQTLEGAAHQFAEKFKKEHGLE
                     IVFDETAVRRLVERAQTERMTMSDLCAHLFRDYQFGLSLIKKNTGRTQFVIDAGAVDA
                     PDKFLSELVVHSYYPAAMAQKA"
     gene            6008..7285
                     /locus_tag="VE486_03575"
     CDS             6008..7285
                     /locus_tag="VE486_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38198.1"
                     /translation="MKRSLFTTLIIGGAIAAIVSVLHATGLLLGLETAAGRLVSDYAG
                     ATRVVGEKWQYVFVSLLAFGVAWLSLGPAAAGPRGGTRLLIVFLIIELLGLAWVCSLY
                     RIFFQPVPSVFAVALAWVATEGWTAFLRWDRSHLMQAIFGKRLPKKEFRGVSVGTISF
                     DAQPETRDVSVVVCDIANKYGLAEDSGPAAFARTLGEFIRDTADRLVRAGAYLQAADG
                     EGVVAIFGFPVADSGHAEKSLRVVLDLIRGFRNRQQDNGEIAADCGIHAGVSSGAIIA
                     APLKDSGSPSLLISGEPLDLARKLCIANRRYGSKILMDTPTFDLAGNIVVARPIDFVN
                     GVNSQDRHEIYEPLWLATEAGPEQIARRDSFWSGVVLYREKRWAEAYAEFQKARGSEE
                     EEDPPLQFYLRRLELRALSSLIETPPESGSSGL"
     gene            7303..7908
                     /gene="recR"
                     /locus_tag="VE486_03580"
     CDS             7303..7908
                     /gene="recR"
                     /locus_tag="VE486_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZA38199.1"
                     /translation="MRKTEYPAAIRNLIAQLRQMPGVGPRSAERIALWIVRAQSDQPE
                     QISRAIADTRTAIHPCALCGFFTTDEICEICADTSRSLELLCVVEQPTDILPLEKTSA
                     FRGRYHSLGGKISPLDHVGPEDLRIKELLERLDREKISEIIFALAADVEGEATTNYLV
                     DLLKGRPVTLTRIARGLPAGGGLESADELTLSAALSGRTKL"
BASE COUNT         1737 a         2078 c         2353 g         1785 t
ORIGIN      
        1 tgacgatgcg tctctttgtg cagacaatga atttcgctga aggcgcaatc gggctgggcg
       61 tcgatgaact gcgctggccc aacgtggtgc gaccgggtga tgtgctaacg gttgaaacag
      121 agattgtcga tcttcggccg tcgcgctcga aaccgaacta cggaatcatt cggctgcgga
      181 acgtgacgac aaatcagcgc ggcgaagttg tgcaaacgat gctggcaagc gcaatggtgc
      241 cgcggcgagg gtagggaagt gagtggtgaa tggtgaatag tgaatattga cgcaggctcg
      301 gtagcccgcg cgccgcacag gcaagatgcc tatgttccga actcaccgat tgaccatctg
      361 catcgcccaa tcgggataac gttcgccggc gaaggcttct ttcggcgcca cttcctcgat
      421 gcgcgcgaga tcggcgctgg tcagagcgac atttagcgcg ccggcatttt cctgcagata
      481 tttgcgccgt ttcgtaccgg ggatcggcac gatgtcgtcg ccctgtgcca gcacccaggc
      541 aagcgccaat tgcgccggcg tacatttttt ctcgcgcgcg atctcgccaa ctcgctcgac
      601 caaatcgaga ttgcgctgaa aattttcacc ctgaaatcgt ggggtcgtgt gccggtaatc
      661 gtctttcggc aaatcctcag gcttttggat tttgccggtt aaaaacccgc ggccaagggg
      721 gctgtaaggc acaaacccga tcccgagctc gcggcagaca ccaagcactt ctgtttcggg
      781 gtcacgcgtc cagagcgaat attccgattg caccgcagtg atcggatgaa cggagtgcgc
      841 ccgccggata gtctccgcgc ctgcttcgga tagaccgagg aatcgcactt tgccctggcg
      901 aaccaattcc gccatcgcgc caactgtttc ttcaatcggc gtgtctggat caactcgatg
      961 ctgataataa agatcaatta catcgacacc gagccggcgc agacttcctt cacatgcctg
     1021 atggacataa tcgggtttgc cgctgatccc gcgaaattct ggcctggccg gatcccgaac
     1081 aatcccgaac tttgtcgcaa taatgacctg ttcgcggtgt cctcgaaggg cgcgtccaag
     1141 caattgctcg ttggtgtgag ggccgtacat atcggcggta tcgaagaatg taattccgag
     1201 ctcaagcgcg cgatcaatcg tagccagaga ttctgcttca tcgcgtccgc cgtaaaaatc
     1261 ggacattccc atgcaaccga gaccgattgc cgagacgatc ggcccgccgc gtccaagttt
     1321 tcgctgtttc ataaaggaag gaacgaacta gaataacagt gagcaagttt catttcagaa
     1381 acgacgcttc gcgtcgccat cggctggcag ccggggatcc aggctggcag cctgtgctcc
     1441 ccagcgttcc aagttttcgt cgtttcatac tctttaggat tcggttgtca gttttagcgc
     1501 ggtcgcgaaa tcatcaggca atggcgcttc aaagtttctc cattcgccgg aaggtggatg
     1561 gcaaaatcca agtttccaag cgtgcaacat ctgtcgcggg aagtcttttg cctggcgtgc
     1621 cgcataaact ttgtcgccca acactgggtg accaatatga tgaagatgca cccggatctg
     1681 gtgggtgcgt ccactgtgca accggcactc gatcaaactc gcctggcctc ccgagcgaac
     1741 aacgcgatac tcggttttgg ctgcgcgccc gcgtaatgag gtcacggtca ttcgttgccg
     1801 ataaaccgga tgccggcgga ttttttcttc gatagcgccc gtttgtttgc gcaattttcc
     1861 cgccacgagc gcgaggtaaa tcttctcgac tgtgcgctcg gcaaactgtc tggacaaatc
     1921 ccgatgagtc acatcatttt tcgcgacgac aaggcagccg cttgtttctt tgtcgaggcg
     1981 atgaacaata ccgggccgtt cttttccgcc gattcctgac agggtcgtgc aatggctgag
     2041 caatgcattg accagagtgt gttgctgatg gccagcgccc ggatgaacga caagcccagc
     2101 cggcttattg ataacgatga gatccttgtc ctcgaacaga atctcgagcg gaatcggctc
     2161 tggctgattc gcgatctttt cgagtggcgg ctcggttagt tcgatttcat cgccgatgcg
     2221 aacagattgg cgtggccgcg tggttacgcc gttaagccga acgaatccgg cgcggatcaa
     2281 atgttgaaga cgcgagcgcg agtattccgg aaggtgcttc gccagaaaac gatcgaggcg
     2341 cgattttgca tcttctcttg gaacagagag ttcgattctg gtgagctttt cggttgaaga
     2401 tcgcctcata catcgtgtgc tcgatggagc agggttctga gcctctcgca gcgaacatcg
     2461 ttttacaata ctcccgcgtc tcagcgttgg gaattgctgt cgcgatttcg ttgccgtgaa
     2521 agtttgggat gttacaatcg ggagtcatgg cgattgaggg aggcgcccag acatgcccgg
     2581 cctgtggcgc gcccgtcgat acgagcgatg cggagccgct ggcgtacatt gcgtgtccga
     2641 actgcgggga aaaaatccgc gtggagcgaa cgttcgatca tttcgttgtg gtcgagacgc
     2701 tcggtattgg cgggatgggc gccgtttata aagcgcgcga tacggagtta gaccggctcg
     2761 tagcgctgaa gctgttgcgc aaggatctca gcggcgaagc ggatcacaat gctcgattgc
     2821 aacaggaagc gcgcgttgct gcctccgtga gtcatcccaa cgtgatacag gtctttgatt
     2881 ctgggacaga tcacggccgt ttttatgtgg tgatggagtt ggtcgatcgt ggaagtctcg
     2941 acgatctcat ggagcggcga aatcagttgg cggaaatgca cgtgcttgag atcggtattc
     3001 aggtcgcaaa aggactgcgt gcggctcacc ggcagggtct gatccaccgt gacgtgaagc
     3061 cagccaacat tttgtttgtc gatgaatacg aagcaaagat tagcgacttt ggtctggctg
     3121 gcgtcgcagg ggaagagtcc gaaacggcgg ccgaaatttg ggggacgccg tactatgtcg
     3181 cgccagaacg attacgcaaa gaaccggagg atttccgggg cgacatttac agtctgggcg
     3241 caactctttt tcacgccgtc gcaggcacag caccgattga aggcgacaca aattctgggg
     3301 ctgtgctgct cgatttgaaa aaacggccgc tcgatttgcg tgacattgcg ccggaggtgt
     3361 ctgaagctac ggccagcatt tttcagcgaa tgatcgcgcc tgatccggcg cagcgttttt
     3421 cttcgtacaa cgaattgctc tcgaatctcg aggaggcgca gcgagctttg atcggcagaa
     3481 cccatattcc cgtcgatcac aggcgacgac caccgcgacg actgcggcgc tggcttgcgg
     3541 tcttgcttat cttgctgggc gcgacggttg cgatgctgtt tgctctgcgt ggacggcaag
     3601 gaaacgctcc tgccagacct ggtccagcgc cgccggttga atccgatgcg gccgaaaaga
     3661 aaattcgcag tgtcgagggc gatccgtgga atagagcgct tgccaattac agacagcagg
     3721 tcgcccttta taactttcct caggcggctg ctgcgattaa aagcgtaaag ctgagcgacg
     3781 cgtcgttcaa gcaggcaaag gaaacaacag aaaaaaaagc gcaatggctg atcgattgga
     3841 agaacgaatt gatctatgat ctcaaccgga ggcatttctc cggcgcattt acgggcagaa
     3901 acggcaccca ctacacgggt atagccagcg cgagcgatga tagccttagt ttgaagctcc
     3961 gatatggcat tacacgagtt gggtggacgc agcttccgcc aaaggcgttg ctcaaggttt
     4021 ccacctcgtt catccaacca acagcgcctg acgcggcaga tcgacaatgg cgttgtgccg
     4081 tctttgcgag cgagactggg caaccggaga gtgcccgggg attggcagag gcggctgcaa
     4141 aggccaaggc ggagtaccgc cagcagattt cacagttgtt tccggatatt ccccagactc
     4201 cttagtgcct gccttactcc tattcttact cttgctctta ctcatattcc gacacttggt
     4261 atgaacagca ccaagaataa gagcaggagc aaaagtaaga atagaaataa gagacagttc
     4321 gaggatgaac gaattcggcg caggagagtc agattttaga cgaatcgttg ccatcgagcg
     4381 acttcggcat gatgttttga ccctgagcat ggagcaaaac ccaccaccat ttccttcacc
     4441 ggaagaactg aagaccaaga tcacggagtt catgaaacag aacttcggcg atcgggtctc
     4501 agtggcgacg tttgccgagc cggagccggc tgcggcagtt gaggatgaga agccggagaa
     4561 gacggagcat cccgaatttg aattcgattt tctgccgcgc gatatcaagg cgcatcttga
     4621 ccgctttgtc attaagcagg acgaagccaa gaaggtgctg gcgatcgcgg tttgcgatca
     4681 ttacaaccac gccaactatc tgcgccggct cgagaaagaa gacgcccggc gcgccgagga
     4741 gaccgagtac atcaagcaaa atgtgatctt ggtcggcccc acgggtgtcg gcaaaacata
     4801 tctcatcaag cacatcgccg atctgatcaa ggtgccgttt gtcaaagcgg acgcgaccaa
     4861 gttcagcgag accggctacg tcggcggcga cgtcgaggat ctcgttcgcg aacttgtcca
     4921 caaagcagat ggcgatgtca atctggcgca gttcggcatt atttatatcg acgagatcga
     4981 caagatcgct tcggcaggaa atctgatcgg gcgcgatgtc agcggacgcg gcgtgcagac
     5041 cacgctgctc aagttgatgg aggaaacgga agtcccagtc cgaagtatga acgatctcca
     5101 ggcgcaattg caggccgcat tcgaatttca gaggcgcgga aaagcgaagc gggacacgat
     5161 caatacgcgg catattctct tcgtcgtgag cggcgcgttc gggaagttaa aagagcaggt
     5221 ggcccgacgc gtcagacaag gtcagatcgg atttagcgct tcgccggtgc aggtgatgga
     5281 taacgagctg tttcaatacg ttacgaccca ggattttatc gagtacggat tcgagccgga
     5341 attcattggg cgtttgcccg tgcgcgtggt gtgcgaggac ctcgatgcgg atgatctctt
     5401 caagatcatg aaatattccg agggaagttt gctacggcaa tacgagcgcg cctttcgcgc
     5461 ctacggcatt cagatcagtt tcgaagatga agcgttgcgc ttgctggccg aagccgccgc
     5521 gaaggaaaag accggcgcgc ggggattatt gacggtattc gaaaaattat tccgcgatta
     5581 caaatatcat ctcgccgggt cgggtttgag ccagttgcgc gttacagccg gcctcgtccg
     5641 cgagccaaaa cgggtgctcg accggttgat ggccgagggg cacaagctgg aagtgcaaac
     5701 cctcgaaggc gccgcccatc aattcgccga gaaattcaag aaggagcatg ggctggagat
     5761 tgtttttgat gagacagcag tgcgacgctt ggttgagcgc gcgcaaactg aacggatgac
     5821 gatgagcgat ctgtgcgccc acttgttcag ggattaccag tttggactga gcctgattaa
     5881 aaagaacacc ggccggacgc agtttgtcat tgatgccggg gccgttgacg ctccggacaa
     5941 attcctgagc gagctggtcg tccactccta ctacccggct gcgatggcac aaaaggcctg
     6001 attgttcatg aagcgatcac tgttcacgac gctgataatc ggaggtgcca ttgccgctat
     6061 cgtcagcgtc ttgcatgcaa cgggactgct tctcggtctt gagactgctg ccgggcggct
     6121 ggtatctgac tatgccggcg ccacgagagt cgtcggtgag aaatggcaat atgtttttgt
     6181 ttcgctgctg gctttcgggg tcgcctggct cagtttgggt ccggcagccg ccggaccacg
     6241 aggcggcacc cgcttgctca tcgttttctt gattatcgag ttgctcggcc ttgcgtgggt
     6301 ctgctcgctt taccgcatat ttttccagcc ggtgccgtcc gtttttgctg tggcgctcgc
     6361 atgggttgcg acggagggat ggacggcttt tttgcgatgg gatcgctcgc atttaatgca
     6421 agcgatcttt ggcaaacgct tgccgaagaa agaattccgt ggtgtcagcg tggggacaat
     6481 ttcgttcgat gctcagccgg aaacgcgcga cgtgagcgtg gtggtgtgtg atatcgctaa
     6541 caaatatgga cttgccgagg attccgggcc cgccgctttt gccaggaccc tgggagaatt
     6601 tattcgtgat acggccgatc gtctcgtgcg agcaggagcg tatttacaag ccgcggacgg
     6661 ggagggagtg gtcgccattt ttggctttcc cgttgccgac tccgggcatg ccgagaaatc
     6721 cttgcgcgtg gtcctcgatt tgattcgggg ttttcgcaat cggcagcagg ataacggtga
     6781 gattgctgca gactgcggca tccatgccgg agtcagttcc ggcgccatca ttgccgcccc
     6841 gttgaaggat agtggaagtc cgtccctttt aatcagcggc gaaccactcg atctggcgcg
     6901 caaactttgt atcgcgaatc gtcggtatgg atcgaaaatt ctaatggaca cgccaacttt
     6961 tgatctggcc ggcaatatag tcgtcgcccg gccgatcgat ttcgtgaacg gagtcaattc
     7021 gcaggatcgg catgaaattt acgaaccact ttggcttgca accgaagccg ggccggaaca
     7081 gatagcgcga cgcgattctt tctggagtgg agtcgtcctg tatcgagaga aacgctgggc
     7141 ggaagcgtat gcggagtttc agaaagcgcg cggttcggag gaagaagagg atccccccct
     7201 gcaattttat ttgcgccggt tggagctccg agcgctttcg tcgttaatcg aaacgccgcc
     7261 cgaatcaggg agcagcggtc tttagtccgc cgtttttctg ccttgagaaa gacggaatat
     7321 ccggcggcga tccggaattt gattgcgcaa ctgcgacaaa tgccgggagt tggaccgcgt
     7381 tcggcggagc ggattgcgct ctggattgtg cgtgcgcaga gcgatcaacc ggagcagatc
     7441 tcgcgtgcca tcgctgacac ccgaacggct attcatccgt gcgcgctctg cggatttttt
     7501 accactgacg aaatctgtga gatttgtgcg gatacttcgc gctcattgga attgctctgc
     7561 gttgtcgaac agcccacaga catccttcct ctggaaaaaa ccagcgcgtt ccgcggccgg
     7621 tatcattctc tcggagggaa aatttcgccg ctcgatcatg ttggcccgga agatttgcgg
     7681 atcaaggaat tgctcgaacg tctcgatcgc gaaaagattt ccgaaatcat tttcgcgctg
     7741 gccgccgatg tcgaaggcga agccacgaca aactatcttg tcgatcttct aaaaggcagg
     7801 cccgtgactt taacgcgcat tgcgcgcggc ctgcccgctg gcggcggcct tgaatcggct
     7861 gatgaattga cgctttctgc cgcgctgtct ggtcgaacaa agctctaacg catggggtgt
     7921 catcccgagg ccgcttagac ggcgagggac ctc
//
LOCUS       DASQUQ010000125         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1111573, whole genome shotgun sequence.
ACCESSION   DASQUQ010000125 DASQUQ010000000
VERSION     DASQUQ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111573"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VE486_03585"
     CDS             <1..431
                     /locus_tag="VE486_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38200.1"
                     /translation="PKAGESPSPSPNQEKQGDQQQKGEREESSPTPGPASTAPAQSRE
                     QSGKEQASPSPGEGENAGPSATPAESPQKKFAGEVKGAGDGKSEKPPDKDAQLGEAEL
                     EKEGQMSERQAEALLESMKDEEARVQLNERRATRRVYNDW"
     gene            477..2330
                     /locus_tag="VE486_03590"
     CDS             477..2330
                     /locus_tag="VE486_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HZA38201.1"
                     /translation="MYRSRICIAVLLGASIVAAGHSVAFADSPSVTAVLSNSEAVVGE
                     MVELQIKVSGSRDAEPPEEIVVNGLEIHATGTSRQFEMRNFTTTSSVTYNYRILPLQA
                     GRFTIPPQIIQVRGKSLQTPELTLNVADSPGGSSASRPSQGAQPGNASRLGFAELIVP
                     KKTAFVGEMVPVQIRLGFDPRAYPEFDVPEITGQGFTAQKLLQSGRNQETIDGRIYLV
                     WTFKTAIAAARAGKFEIGPVKAKAQVVVSRSRRSPRGRSPFDLFSMDDPFSDPFFANP
                     FGRFGERREVEVKSDPVPLEVKPLPPGAPADFSGAIGNFTMTTEAKPKSVQVGDPITV
                     TSAISGRGNFDRVGAPVIENERGWHKYPPSSKFAKDDEVGISGTKTFESVLSPNEKKE
                     SLPVMEFAYFDPVKEQYVTLRSDPIAISVQGGAVAAAVPPGSPATASPPGSPTPTTVQ
                     KPQDILYQLPERPKVAQSFTPLYARPIFWAGQLIPLLALLGLIGWKIRQSRIGNREAR
                     RIAALQHEAAELMRKLHRGDASPREYYAEASRVVRVKAALASGGRQIDPNTVDVETAA
                     DTFRLNGDSRERLRRLFEQSDELQYSGAHNGAEKISPEIRRDVLELIENLK"
     gene            2429..>2733
                     /locus_tag="VE486_03595"
     CDS             2429..>2733
                     /locus_tag="VE486_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA38202.1"
                     /translation="MTRNRTLAIFFCLTFGIASYTFAQADVRFAKANQDFAQGNFKEA
                     ISGFETLVRSGQWSANVFYNLGNAYFRAGDFGRAILNYERALALERHHPEATANLQIA
                     "
BASE COUNT          635 a          727 c          820 g          551 t
ORIGIN      
        1 aaccaaaggc gggtgaatct ccttcgccgt cgccgaacca ggaaaaacaa ggagatcagc
       61 agcaaaaagg cgagcgcgaa gagtcctcgc ctactcccgg gcccgcgtct acggccccgg
      121 ctcaatcgcg agagcaaagt ggcaaagaac aggcatcgcc gagcccggga gagggcgaaa
      181 acgcaggacc atccgctact ccagcggaat cgccgcagaa gaaatttgcg ggcgaagtga
      241 aaggcgccgg cgacggcaaa tcagaaaaac caccggacaa agatgctcaa ctgggggagg
      301 ccgaattgga aaaagaaggt cagatgagcg aacgtcaggc cgaggcgctt cttgaatcga
      361 tgaaagatga ggaggctcgg gttcagctta atgaacggag ggcaacgcgt cgtgtctata
      421 acgactggta gggcccgtgt ttgatgtttg agggttgatg tttgattgaa tattcgatgt
      481 atcgatcacg aatttgcatt gcagttttgc tcggcgcctc aatcgtggcg gcaggacaca
      541 gtgttgcatt tgctgattcg ccgagcgtca cggcagtctt gagtaactcg gaagccgttg
      601 ttggcgaaat ggtcgagttg caaatcaaag taagtggttc ccgggatgcg gaaccgccgg
      661 aagagatcgt agtcaacgga ttggagattc atgccaccgg gacatcgcgc cagttcgaaa
      721 tgcgcaattt cacaactaca tccagcgtga cctacaacta ccgaatcctg ccgctgcaag
      781 cgggccggtt tacaatcccg cctcagataa tccaggtgcg cggcaaatcg ctacagacgc
      841 cggagttgac gctcaacgtc gccgattcgc ccggcggttc ctctgcttct cgtccaagtc
      901 aaggcgcgca gccggggaac gcgagcagac tcggtttcgc cgaactgatt gtgcccaaga
      961 agaccgcctt tgtcggcgag atggtgcctg tccagattcg gctgggattt gacccgcgcg
     1021 cgtatccaga attcgacgtt cccgaaatca cgggtcaagg atttaccgcg caaaagctac
     1081 tacaatccgg aagaaaccag gagacgattg acggtagaat ctatcttgtc tggacattta
     1141 agaccgcaat cgctgcagcg cgcgcgggaa aatttgagat cggtccggtt aaagcaaagg
     1201 cgcaagtggt ggtctcgcga agccgccgct cgcctcgagg gcgttccccg ttcgacttgt
     1261 tcagcatgga cgatccattc tcagatccat tctttgccaa tccgtttggc cgatttggtg
     1321 agcggcgcga ggtcgaagtc aagagcgacc ctgtgccact cgaagtaaaa ccgttgccgc
     1381 caggcgcgcc ggcagacttc tcaggcgcga ttgggaattt tacgatgaca acggaggcga
     1441 agcccaagag tgtgcaggtt ggcgatccga ttactgtcac atcggccatt tcggggcgcg
     1501 gtaatttcga tcgtgtcggt gcgccggtga tcgagaacga gcgcggctgg cataagtatc
     1561 cgccatcttc caagtttgcg aaggatgatg aggttgggat aagcggcaca aaaacgtttg
     1621 agagcgtgct ttcgccgaac gagaaaaaag aaagcctgcc ggtgatggag tttgcctact
     1681 tcgatccggt gaaggagcaa tacgtgacgc tgcgcagcga cccgatcgcg atcagtgtac
     1741 agggtggcgc ggtcgcggcg gccgtcccgc cggggtcccc ggcgacggcg agtccgccgg
     1801 ggtcaccgac cccgactaca gttcagaagc cgcaggatat tttgtatcag ctgcccgaac
     1861 gacccaaagt cgcgcaatct ttcacgcccc tttacgcacg accgattttc tgggcggggc
     1921 agttgattcc gctgttggcg ttactggggt tgatcggttg gaaaattcgg caatccagga
     1981 tcggtaatcg cgaggcgcgc cggatcgctg cgcttcaaca tgaagccgcc gagctaatgc
     2041 gcaaattaca tcgcggcgac gcatcgcccc gcgaatacta tgccgaggct tcccgagttg
     2101 ttcgcgtaaa agctgctttg gcgtccggtg gccgccagat cgatccaaac acggtggacg
     2161 tcgagactgc ggcggacacg ttccgactca acggcgattc gcgcgagcgg ttgcgccgtt
     2221 tgttcgaaca gtctgacgaa ttgcaataca gcggagcgca taacggcgca gaaaagattt
     2281 ctcctgagat tcgccgggat gtgctggagt tgatcgagaa tttaaaatga actttttcgc
     2341 cgtcattccg agcaaagcga agcgaagtcg aggaatcccg cagagaagtc caacggtagt
     2401 tcaacagggt ccctcgactt cgctcgggat gacgcgcaat cgaacgctgg caatcttttt
     2461 ctgcctgact ttcggaatcg cttcatacac gtttgcacag gcggatgtca ggtttgcaaa
     2521 ggcgaaccag gatttcgcgc agggcaattt caaggaagcg atctccggtt tcgaaacgct
     2581 tgtccgctcc ggacaatgga gcgcgaacgt tttttacaat ctcggcaatg cttattttcg
     2641 cgcgggcgat tttgggcgcg cgatcctgaa ttatgagcga gccctcgcct tggaacgaca
     2701 tcatcccgaa gcgacggcca atttgcagat cgc
//
LOCUS       DASQUQ010000126         4851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1308432, whole genome shotgun sequence.
ACCESSION   DASQUQ010000126 DASQUQ010000000
VERSION     DASQUQ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308432"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VE486_03600"
     CDS             <1..537
                     /locus_tag="VE486_03600"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HZA38203.1"
                     /translation="NAFMLRWILSNYPTESVWQLKDSFSGKRNEIGGRQVSLDDIEHG
                     ALRPLIGYRAHSVLVCAARSCPPLQRFAYTGDKFEEQNDLAYRAWLAREDLNKFLPNE
                     KKAEISSVFKWFKDDFDKAGGVPKTLGLYAPQSVREFAASGKYEIKYISYNWGLNDQG
                     PHGRNYTRAQQLFDNVFK"
     gene            complement(637..1359)
                     /locus_tag="VE486_03605"
     CDS             complement(637..1359)
                     /locus_tag="VE486_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZA38204.1"
                     /translation="MTEKTHERVAIVAGVGPGLGAALVRKLAEEGCRVGMFARSPKFI
                     RELAAELGSKSLAVPTDISDPKQVASGFRKVRERLGPVEILIAHASGSLGGHLEKTSP
                     EQFERSWRTAAFGAFLCAREAVPDMLKRRTGAIIFTGATSSVRGRGGAVAFSSAKFAV
                     RGLAQSLAVEFWPKGIHVAHVIIDGVIDPGKRRKSYKPSAKDPLLQPKAIADAYWHLI
                     EQDRSAWSLEIDLRPHNEAFFE"
     gene            complement(1454..2314)
                     /locus_tag="VE486_03610"
     CDS             complement(1454..2314)
                     /locus_tag="VE486_03610"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZA38205.1"
                     /translation="MPKIPLMDYVGRPLLACLFLALLVLQWRFPLRRQHFSALHRLVR
                     NFVLSIPGFAIVRVAMLPIPFAVAMWTQDRHIGLLNWLALPGWAACIATFLLMDYAYW
                     WWHWANHMVPLFWRFHNVHHTDLDMDVSTAARFHFGEILFSVGFLSLAMVVFGIAPIM
                     LLVFFITFEAATLFHHSNWRLPIQLERVLNLVMVTPRMHGIHHSIVQRETNSNWGTIF
                     CWWDKLHRTLRRDVPQDAITIGVAAYREEHELTVGKLFALPFGKQREWRLPTGEQPER
                     EPQPAEKLAD"
     gene            complement(2514..3455)
                     /locus_tag="VE486_03615"
     CDS             complement(2514..3455)
                     /locus_tag="VE486_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZA38206.1"
                     /translation="MRFLTITAALLANTIAAHGAGEPGVIKGADQFEFVYRFKLPEIK
                     GDARVWLPLAKTDAFQTVTQEELSIPVKWDKVRDRDYANDICVLYPQPADSGKTIELK
                     YRVVRKEKTAYPADGDTTRYLRPETLVPVNDTFRTLALKAVAGKTDDLERAKALYDHV
                     IGRMRYDKSGTGWGRGDALYACDARTGNCSDFHAYFIALARSIGIPARFAIGATIPAD
                     KNEGTIDGYHCWAEFFADGRWVPVDISEGWKNRKLANYYFGHNPANRFELTKGRDLVV
                     DPEPASGPINFLAYPLLEMNGEVHKPETTFMFRRIGA"
     gene            complement(3497..4021)
                     /locus_tag="VE486_03620"
     CDS             complement(3497..4021)
                     /locus_tag="VE486_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38207.1"
                     /translation="MISRLIMAVAVALLFTPAALVCAQEHPEHPKKEKTSEHPKKGAE
                     HPKKGAEHPEKAGAEKEVSTADISAGIKKNIDTQSKKSSDGKFHVKYEGQDLALDLIK
                     VHDDRLQDLSGGKYFACVDMKATDGKTYDIDFFLTGKPGKMKVTDTDVHKIDGKPLYN
                     WKEEDGKWQKVPVS"
     gene            complement(4354..>4851)
                     /locus_tag="VE486_03625"
     CDS             complement(4354..>4851)
                     /locus_tag="VE486_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38208.1"
                     /translation="VLHQRHVGKSANFEEGSSPAEYAVIATSNSKQNPCVMSKAVRES
                     INKASRQANSEITANDIRIIHDARDLIQTLQWQLSIDMDKPKDLPSRGMRAGVHLFET
                     TGNTLDQPITKSGSEPSCPIGASPVRDNNLGFWRSPAEMLKKWSYQSRLVEYRDNDRD
                     LRPLL"
BASE COUNT         1028 a         1353 c         1358 g         1112 t
ORIGIN      
        1 aacgctttca tgctccgttg gattttgtcc aactatccca cggagagcgt ctggcagtta
       61 aaggattcgt tttccggcaa acgcaacgag atcggcggcc gccaagtttc gctggacgac
      121 attgagcacg gagcgctgcg accgttgatt ggttatcgcg cccattctgt gctcgtctgc
      181 gcggcgcgca gctgtccgcc actccagcgc ttcgcctaca cgggcgataa attcgaagag
      241 cagaatgacc ttgcatatcg tgcatggctc gcgcgcgagg atctcaataa atttttgccg
      301 aacgagaaga aggccgaaat ctccagcgtc ttcaaatggt tcaaagacga cttcgataag
      361 gcgggcggtg tccccaaaac tctgggccta tatgcgccgc aatcagtgcg tgaatttgcc
      421 gccagtggca aatacgagat taaatatatt tcctacaact ggggacttaa cgatcaggga
      481 ccgcatggcc gcaactacac ccgggcgcaa cagctcttcg acaatgtttt taaataggcc
      541 gcagcgtgag cgacggttcc ggttgcagcc gtggtagggt cgtcgcgctg cgacgaccgg
      601 acgcctcagc gggcgtccct gccagatata aacgcgtcat tcgaaaaacg cctcgttgtg
      661 cgggcgcaga tcgatttcca agctccaagc gctgcggtct tgctcgataa gatgccagta
      721 ggcatcggcg attgctttgg gttggaggag gggatctttc gcagaaggtt tgtagctctt
      781 tcgcctcttt ccggggtcga tcacgccgtc gataataacg tgcgcgacat ggatgccttt
      841 aggccagaac tcgaccgcca gtgattgagc cagaccgcgc acggcaaact ttgcgctgct
      901 gaatgcgacc gctccgccgc gcccgcggac agacgaagtc gcgcctgtga aaatgatcgc
      961 tcctgtccgt cgcttgagca tgtcaggcac cgcttcgcgc gcacacagaa atgcgccgaa
     1021 tgcagccgtg cgccaggaac gctcgaattg ctccggagaa gtcttttcca agtgcccgcc
     1081 gagcgaaccg cttgcatgcg cgatgagaat ctcaaccggg ccgagtcttt cgcgcacctt
     1141 gcgaaaaccg gatgcgacct gtttcgggtc cgagatatcc gtgggaaccg cgagcgactt
     1201 tgaccctaac tcagcggcga gttccctgat aaacttaggc gaacgagcga acatgcctac
     1261 gcggcagcct tcctccgcta gcttcctcac cagggcagct ccaagtccag gaccgactcc
     1321 ggcgacgatg gccactcgtt catgcgtctt ctcagtcata tccttttcac tcgcattcac
     1381 ggtgagattc atccttggca agcagtttcg gtgggctacg cggagcgcat gccctacaat
     1441 ggcgttcgcg aaattagtcg gcgagctttt ccgctggttg cggctcgcgt tcgggctgtt
     1501 caccagtcgg caaacgccat tcgcgctgtt tgccaaatgg tagcgcgaag agtttcccga
     1561 cggtgagctc gtgttcctcg cggtatgcag ccacgccgat ggtaatggcg tcttggggca
     1621 cgtcgcggcg cagcgtccga tgaagtttgt cccaccagca gaaaatcgtg ccccagttcg
     1681 agttggtctc tcgctggacg atggaatgat gaatgccgtg catccgcggc gtgaccataa
     1741 cgagattcaa aacgcgttcg agttgaatcg gcagccgcca attggaatga tgaaagagag
     1801 tagcggcttc gaatgtgatg aagaaaacga gaagcatgat cggagcgatg ccaaaaacaa
     1861 ccatggcgag cgacaaaaag ccgaccgaga aaaggatttc gccgaaatgg aatcgcgcgg
     1921 cggtactcac gtccatgtcg agatcagtgt gatgcacatt atgaaatcgc cagaagagcg
     1981 ggaccatatg gttcgcccaa tgccaccacc aataggcgta atccataagc agaaaggtgg
     2041 cgatgcaagc ggcccatccg ggcagagcga gccagttcaa aaggccgata tggcgatctt
     2101 gggtccacat cgccaccgcg aaggggattg gcagcatggc aacgcggacg atggcgaaac
     2161 cgggaattga gaggacaaag ttgcgcacga gccggtgcag cgcgctgaaa tgctggcgtc
     2221 gcagaggaaa gcgccattgc agcacgagca gcgcgaggaa taggcaagcc agcaatggtc
     2281 tgccgacgta atccatcaag ggaatttttg gcattggatt ggagacggac tttcgaacca
     2341 cctatcgagg tgcgcacaac atagtgtcgg gcgagggcga aagcgatgcg aagacgcatc
     2401 gcattccaaa agcacttcgt gcgaaattta tcgcaatacg cctgctcttt ttcgcgaagc
     2461 tttgggagtg cgcacgcgtc ctcgcgtcgc ttttcggggc agtcagcaac gccctaagcg
     2521 ccgatgcgcc ggaacatgaa tgtggtctcg ggcttatgga cctcgccatt catctcaagc
     2581 aacgggtagg cgagaaaatt gatcggccca ctggccggct cgggatcgac caccagatcg
     2641 cggcccttcg tcagctcgaa acgattagct gggttatggc caaagtaata attggcaagc
     2701 ttgcgattct tccatccttc gctgatgtcc acggggaccc agcgcccatc agcgaaaaat
     2761 tcggcccaac aatgatagcc atcgatggtg ccttcgtttt tatcggcggg gatcgtagcg
     2821 ccgatggcga aacgcgccgg tatgccgatg ctgcgcgcca acgcgataaa ataagcgtga
     2881 aaatcactgc aatttcctgt gcgcgcgtca catgcgtaga gcgcatcacc gcggccccag
     2941 ccggtgccgg atttatcgta gcgcatccgg ccgatcacgt gatcgtacaa tgctttcgct
     3001 cgctcaaggt cgtctgtttt tcccgccacc gctttgagcg caagcgtccg gaaagtgtca
     3061 ttcaccggca caagcgtttc cgggcgcaga tagcgcgtgg tatccccgtc cgcgggatac
     3121 gcggtcttct cttttcgcac gacgcgatat ttcaactcga tggtcttgcc gctgtctgcg
     3181 ggctgaggat agagcacgca aatatcgttg gcgtaatcgc ggtcccggac cttgtcccat
     3241 ttcaccggga tactcagctc ctcctgggtg acggtctgga aagcatccgt cttcgccagc
     3301 ggtagccaga cgcgcgcatc gcctttgatc tccggcagtt tgaaccggta aacaaactcg
     3361 aactggtcgg cgcctttgat aacgcccggc tcaccagcgc catgagctgc aatcgtattc
     3421 gcgagaagag cagccgtgat cgtcaggaag cgcatggaag tcctaattgg taatccgcat
     3481 tcccgaaagt ttcgggtcag ctcaccggca ccttttgcca ctttccatct tcttctttcc
     3541 agttgtagag cggcttgcca tcaatcttat gaacgtccgt gtctgtgact ttcattttgc
     3601 ccggctttcc ggtcagaaag aaatcgatgt cgtaagtctt accgtccgtc gccttcatgt
     3661 ccacgcacgc gaagtatttt ccgccgctga gatcctgcaa ccgatcatcg tgcactttta
     3721 tcaggtcgag cgccagatcc tgaccttcgt acttcacatg aaacttccca tcgctgctct
     3781 ttttgctttg agtatcgatg tttttcttga tgccggccga aatatcagcc gtgctgactt
     3841 ctttttctgc cccagctttt tcggggtgtt ctgcgccctt cttcgggtgt tccgcgccct
     3901 tctttggatg ctccgaggtt ttctctttct tcggatgttc tggatgctcc tgcgcacaga
     3961 ctagcgccgc tggtgtaaag agcagggcca ctgccactgc catgataagt cttgagatca
     4021 taatatgaat tcctccattt ttagcttcgt atgctcacag ttggttggat gtctcgatag
     4081 ttttctggtg ctctcactca ttagtgataa cgacggacgg cttgggttga cctcgctacg
     4141 gcgcgcccga cggtcacgtc ctaccaaatt gcctcgattt gttcatgaga gccgccactc
     4201 aggcgtttta ttcctgtttc actgcaaagt tctttgccaa agccggttct gggcgaaaat
     4261 tggtgagtga tcagccgccg atcggtattc gtagaacgca gcgatccagt ttcagcaaga
     4321 atacgatcgt ctgatcgcga cccgtcagcg gatttagaga agcggacgca ggtctcgatc
     4381 attgtcccgg tattcaacga ggcgggattg atacgaccat ttcttcagca tctccgcggg
     4441 cgagcgccag aagccgagat tattgtcgcg gacgggggaa gctccgatgg gacagctcgg
     4501 ctcgcttccg gattttgtga tcggctggtc gagagtgttc ccggtcgtct caaacaaatg
     4561 aacaccggcg cgcatgccgc gcgaggggag atcctttggt ttgtccatgt cgatgctcaa
     4621 ctgccattgc aatgtttgga tgagatcgcg cgcatcatgg atgatccgaa tgtcgttggc
     4681 ggttatttcc gaattcgcct gccgcgaggc cttgtttatc gactcacgga cagctttgct
     4741 cattacgcag ggattctgct tcgaattcga tgtggcgatc acggcatatt ctgccggcga
     4801 actgccttcc tcgaaattgg cggatttccc gacgtgccgt tgatggagga c
//
LOCUS       DASQUQ010000127         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_390363, whole genome shotgun sequence.
ACCESSION   DASQUQ010000127 DASQUQ010000000
VERSION     DASQUQ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390363"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1136)
                     /locus_tag="VE486_03630"
     CDS             complement(12..1136)
                     /locus_tag="VE486_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA38209.1"
                     /translation="MKHQPFRGLGAILFKEFIVVWRDPMTLFFMFFPPLIEMVAFGYA
                     LDNDVKHMATIILNEDRTVESRQLIDRFVNTQTFRVVGEVQSLEEMKSELRKGRAYVG
                     LQIPPTFTRDVRAGRSAQVQLLVDGSNSTTALQALNTGMGVALTQSVESLLRETGRQD
                     VPIDIRPQMLYNPAMRSPNFFVPGVIGVVLQIGTTVATAMALVRERERGTLEQLLVSP
                     LSRAGLMLGKLVPYLCIGMAMAVILFLIMRFVFDVPIVGNVVGMLFSTLVYVFALLSL
                     GLLIGTKAENQMQALQMSMVFLLPSVFFSGFIFPRETMPWIFYALGALFPTTYFIALM
                     RAIILRGAYFFEYWPNLAILILMSIVLFVLCALRFRKKIA"
     gene            complement(1260..2228)
                     /locus_tag="VE486_03635"
     CDS             complement(1260..2228)
                     /locus_tag="VE486_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA38210.1"
                     /translation="MAPEQQPMIECEGLTKRFGTFTAVDHVTFSVGKGSIFGFLGPNG
                     SGKSTVIRMLCGILEPSDGTARIGGHDIVREMEPIKEMIGYMSQQFSLYDELTVNENL
                     IFSGKLYGLRGRELNQRRDELIALTHLEPYIDRRAGLLSGGWRQRLAMACSLMHKPTV
                     LFLDEPTAGIDPVARRELWDLLFEFAGKGMTLFVTTHYMDEAERCDHVGYIYMSKLIV
                     CGEPDELKQMPEVNPPGTRRLDVTCDHVTTALHAMHQLRGVRSATVFGQSMHLLVDES
                     VKRAQIDEQLRSVGVGHSEIHEIGPSLEDVFVELSAKHATEQQKAA"
     gene            complement(2331..3206)
                     /locus_tag="VE486_03640"
     CDS             complement(2331..3206)
                     /locus_tag="VE486_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZA38211.1"
                     /translation="MNASFVRDDRRLMRAAGAFTILLLLAGCSNREKAVSGTIEVDEV
                     HVGPRSGGRVEKIFAWEGDRLHAGQPIVELDASELRARRDFAAAQIDTAIHDADAQQA
                     QLEFLRDDARRQQDLLKRKVVSPSDAERADSSAKAQEKNVAAAKMRVAQARAQLADID
                     AQLAEMQVVAPSDSILEVLSVKVGDVLPGNREVATLILPQHLWVRVYVPEPWLGHIKV
                     GDQVRVRVDSFPGKDFDGTVEQINRQAEFTPRNVQTVADRVQQVFGVKIRLPSQDERL
                     RAGMAADVYFPNVKR"
     gene            complement(3203..3829)
                     /locus_tag="VE486_03645"
     CDS             complement(3203..3829)
                     /locus_tag="VE486_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZA38212.1"
                     /translation="MKPANKPDSRRRKRDPTRQRIVDAARIHFFSHGFRSVTMDDLAE
                     ELGISKKTLYAHFPGKIDLLEAVLVDKFAGVEATLKKVTRAHPHDFPAALQDLLAGTH
                     RELDEIKPPFVRDMRQKAPDFFKIVERRRAALIQRYFGKFFGEGQRAGMVRKDVPVKL
                     IIEILLAMVQSIMNPPKMEELGMMPKEGFRGILKIILEGALTAKGRKA"
     gene            3986..4471
                     /locus_tag="VE486_03650"
     CDS             3986..4471
                     /locus_tag="VE486_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38213.1"
                     /translation="MNGYSGTPLAQKLGIKPAMTVTVINEPADYRQLLGEGADDVQFS
                     DRTRADSSFVHLFTTRCSELEKQLSRLRKKIPDTATVWVSWPKKSAGISTDVTEDVIR
                     AVALPLGFVDVKVCAVDETWSGLKLMVRREQRKSQPLISRFASSSSPGEEWGEGTGGK
                     K"
BASE COUNT         1047 a         1274 c         1167 g          984 t
ORIGIN      
        1 aaaaagcccg attacgcgat cttctttcga aatcgcagag cgcagagaac gaaaagcacg
       61 atcgacatca ggatgaggat ggccaggttc ggccagtatt cgaaaaaata tgcgccacgc
      121 agaatgatcg cgcgcataag cgcgatgaaa taagtggtcg ggaagagggc gccgagagcg
      181 taaaaaatcc agggcatggt ttcccgcgga aaaataaagc cggagaaaaa aacgctcggc
      241 aagaggaaca ccatcgacat ctgcaaagcc tgcatttggt tttcggcctt ggtcccgata
      301 agcagaccaa gactgagcag cgcgaaaaca taaaccagag ttgaaaacaa cattcccacg
      361 acattgccaa caatcggaac gtcgaacacg aagcgcatga tgagaaaaag aatcaccgcc
      421 atggccatgc cgatgcacaa atagggaaca agtttgccca gcatcagccc cgcgcggctc
      481 aatgggctta ccagcaattg ctcgagagtc ccacgttctc tttcgcggac aagcgccatg
      541 gcagtagcca cagttgtgcc aatttgcaga accacgccga tcacccctgg cacaaaaaaa
      601 ttcgggctgc gcatcgccgg gttgtaaagc atttgtgggc gaatatcgat gggaacatcc
      661 tgccggccgg tttcgcgcag aagcgattcc actgattggg tcagggcgac gcccattcct
      721 gtgttgagcg cctgcaatgc ggtggtggag ttcgatccat ccaccagcaa ttgcacttgg
      781 gcggatcggc cggcgcggac atcgcgggta aacgtcggcg gaatttgcaa gccgacgtag
      841 gcgcggccct tgcgcaattc gcttttcatt tcttcgaggc tctgcacctc tcccaccaca
      901 cgaaatgttt gcgtgtttac gaagcgatcg atcaactgac ggctctcgac cgtgcgatct
      961 tcgttcagga tgatggtcgc catgtgtttc acgtcgttgt cgagggcgta gccgaaggca
     1021 accatctcga tcaggggcgg aaaaaacatg aagaaaagcg tcatcggatc acgccagacg
     1081 acgatgaatt ccttgaagag aattgcgccg agcccgcgaa aaggttgatg cttcatttct
     1141 taaccccgaa aaaaattggc cacagattga cacagatttt cacagatttg agataaagca
     1201 gaaatgtacg cgctctgttt cttatttgtg tttcatctgt gttcatcggt gggtggtact
     1261 cacgcggcct tctgttgttc ggtcgcatgt ttggccgaaa gctcgacaaa cacatcttcg
     1321 agcgaaggcc cgatttcgtg aatctcggag tgaccgactc cgacgctacg caattgttcg
     1381 tcgatctgcg cgcgcttgac actttcgtcg accagcaaat gcatcgattg gccaaagacc
     1441 gtggcactgc gcacgcctcg aagctgatgc atggcatgaa gcgctgtcgt cacgtgatcg
     1501 caagtcacat ccaggcgccg ggttccgggc ggattcacct cgggcatttg ctttaattca
     1561 tccggctcgc cgcacacgat cagcttcgac atataaatgt agccgacgtg atcacagcgt
     1621 tccgcctcgt ccatgtaatg ggtggtaacg aatagggtca tccctttgcc ggcgaactcg
     1681 aagagcaaat cccaaagctc gcggcgcgcc accggatcga tccctgcggt gggttcatcg
     1741 agaaataaga cggtcggctt atgcatgagc gagcaggcca tggccagacg ctgacgccag
     1801 cctcctgaaa gcaacccggc acgcctgtcg atgtatggct cgagatgcgt gagcgcgatc
     1861 aattcatcgc gtcgctgatt cagttcccgg ccgcgcaggc cgtaaagttt gccggagaag
     1921 atgagatttt catttaccgt caattcatcg tagagcgaga actgttgcga catgtagccg
     1981 atcatttcct tgattggttc catttctcgc acaatgtcgt gcccaccaat gcgagcggtt
     2041 ccgtcacttg gctcaagaat cccgcaaagc atgcgaatga cagtcgattt gccggaaccg
     2101 ttcgggccga gaaagccgaa gatcgatccc tttccgacgg aaaacgtaac gtgatcaact
     2161 gccgtgaacg tcccgaagcg tttcgttaaa ccttcgcact caatcatcgg ctgctgttcc
     2221 ggtgccatat ttgcgtcacc acgaaggaca cgaagaacac gaagaaatcg agagggattt
     2281 cttcccaaat tcattccagc tctcgtgatc ttcgtggcct tcgtggttca tcaacgcttc
     2341 acgtttggaa aataaacgtc cgccgccatc ccggcccgca atcgctcatc ctggctgggc
     2401 aaacgaatct tgacgccgaa tacctgctga actcgatcgg ccaccgtttg gacgttgcgg
     2461 ggcgtgaatt cggcctggcg attaatttgc tcaaccgtgc cgtcaaaatc ctttcctgga
     2521 aacgaatcca cacgcactcg aacctgatcg cctactttaa tgtgccccag ccagggctcc
     2581 ggcacataga cccgcaccca caaatgctgc ggcagaatca gggttgcgac ttcgcgattt
     2641 cccggcaaaa catcgcctac tttcacgctg agcacctcca gaatggaatc gctcggcgcg
     2701 accacctgca tttccgctaa ttgcgcgtcg atatcggcca gctgtgcacg cgcctgcgca
     2761 acacgcatct tcgccgctgc gacatttttc tcctgtgctt tggctgagct gtctgcacgc
     2821 tccgcgtcac tcggagaaac gactttgcgc ttgagcaaat cctgctgacg ccgcgcgtcg
     2881 tcgcgcagga attccaactg cgcctgttgc gcatcagcgt cgtgaatggc ggtgtcaatt
     2941 tgtgctgcgg caaaatcgcg gcgtgctcgt agttcggaag cgtccagttc cacgatcggc
     3001 tgaccggcgt gcaatctgtc cccttcccac gcgaaaatct tttccacgcg cccgccgctg
     3061 cgcggtccca cgtgcacctc atccacttca atggttcccg aaaccgcctt ttcgcggttg
     3121 ctgcaaccgg cgagcaagag aaggatcgta aaagctcccg ctgcgcgcat caagcgtcga
     3181 tcgtccctaa cgaatgacgc attcacgctt tccgcccctt tgctgtcagc gcgccttcga
     3241 gaataatctt aagaatccct ctgaagcctt ctttcggcat catcccaagc tcctccatct
     3301 tcggcggatt catgatcgat tgaaccatcg ccagcaagat ttcgatgatc agtttgaccg
     3361 gaacatcctt gcgcaccatt cccgcccgtt gcccttcgcc gaaaaatttt ccgaagtagc
     3421 gctggattag cgcggcccga cggcgttcga cgattttgaa gaagtccggc gccttctggc
     3481 gcatgtcgcg cacgaacggc ggcttaatct catccaattc gcggtgcgtg ccggccaaca
     3541 agtcctgcaa agccgccggg aagtcgtgcg ggtgcgcgcg cgtaactttc ttcagcgtcg
     3601 cttcgacacc tgcgaattta tcgaccagca cagcctcgag gagatcgatc ttgcccggaa
     3661 aatgcgcgta gagcgttttc ttgctaatgc ccagctcctc cgccagatca tccatggtca
     3721 cactgcggaa cccgtgactg aaaaagtgga tccgcgcagc atccacaatt cgctgccggg
     3781 ttggatcgcg ttttcgtctt cgcgagtccg gtttgtttgc cggtttcata tccgtgatgg
     3841 ttttatcaca atgtggaaac taatccagta tatttagttt ccacagtcga ctgccgatat
     3901 tttgatatat cactttaccc tagacagccg gaaaactggc aaaatccttc gacaaagcca
     3961 tgtttgaatc gataacccac taacgatgaa cggttactct ggcacaccgc tggcgcaaaa
     4021 acttggtata aaaccggcga tgaccgtcac cgtcatcaac gaaccggccg attaccggca
     4081 attgctcggc gaaggtgccg acgacgtgca gttttccgat cggacgagag ccgattcgag
     4141 tttcgtccat cttttcacga cgcgctgcag cgaactggaa aaacaattga gccgattgcg
     4201 aaagaaaatt ccggacacgg ccacggtttg ggtttcgtgg ccgaaaaaat cggccggaat
     4261 atcaaccgat gtgaccgaag atgtgattcg cgcggtcgcg ctgccgcttg gcttcgtcga
     4321 tgtaaaagtg tgcgcagttg atgaaacctg gtccggattg aaattgatgg ttcggcggga
     4381 acagcgaaaa tctcagcccc tcatcagtcg cttcgcgtct tcctcttccc ccggagagga
     4441 atggggtgag ggcacgggag gaaaaaaatg aa
//
LOCUS       DASQUQ010000128         4167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1112701, whole genome shotgun sequence.
ACCESSION   DASQUQ010000128 DASQUQ010000000
VERSION     DASQUQ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112701"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="VE486_03655"
     CDS             complement(<1..1613)
                     /locus_tag="VE486_03655"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZA38214.1"
                     /translation="MSLNVEILSRAANEARGLCMDAVQASQSGHLGLPLGCAEMGAVL
                     FGYALKYNPDQPRWINRDIFVLSGGHGSMFLYAWLHMSGYDLPMSEIKRFRQLHSKTP
                     GHPEFGDTPGVECTTGPLGQGVGNSVGISVATKMAAARFNTDEHKIFDQHVICLCSDG
                     DMQEGVASEACALAGHWGLDNLIFIYDSNAVTLDAAAKETQSEDTRKRMEAYGFDVQE
                     IDGHDMQQFLDALNVAKTRDNGKPQFIIAHTLIGKGVPEVEGTYKAHGEAGAKYVDAA
                     RKALGLPEEHYFVSQDVYDYFAEHKKKLIADYDRWEKTYDEWRQKNPGLAKMLDDGIE
                     REVPADLLSKIPEFPKDAKLATRKAGGEVLQPIAQAMPLLMSGSADLYGSTMNYIKEG
                     GDFTRDTPGGRNIRFGIREHGMCAILNGISYHGLFRASGATFTVFTDYCRASIRLGAL
                     AKLPNLYIFTHDSIGVGEDGPTHQPVETVSSVRLMPNIDVIRPADPEETAGAFVAAMQ
                     RTDGPTFLALTRQTVPILNEIDVKLRREGV"
     gene            1834..3009
                     /locus_tag="VE486_03660"
     CDS             1834..3009
                     /locus_tag="VE486_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38215.1"
                     /translation="MNPPSLLQIVERLERLAGRLPSRIRKAVLSELTPLKQLFLQQRP
                     PRFLFAGSSVMPLGQVIAALFASNHPTGIHNVPVRTGHWHDISLPDRGTISILDARGA
                     DASAAIQIRDELKYQPADVIFFLDDGQIGSEPSLHELLSDPAWNKTPEPDAKIIEIIL
                     PSPTAGSEFAPANLVSFRLESVPGSDAAGTVRYLEVLRLVPGSHDRGVESTKGLEVEL
                     PDVESKRLMSVLARELPNQARMEMIRISRNREAQHEVAQLLVKSATAICAAIGAQPIP
                     LADLPILTSLQVMMISGIMYVSGRERSLRAATQFIGALGANVGAAMLLREGTRAILKF
                     FPGWGNVVCGLVAGSGTYAIGRAATVFFIDGVSLKDARRTYLASRKKRARLALPAHE"
     gene            3151..>4167
                     /gene="rsmA"
                     /locus_tag="VE486_03665"
     CDS             3151..>4167
                     /gene="rsmA"
                     /locus_tag="VE486_03665"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZA38216.1"
                     /translation="MKLSELRTTLQQIRVSPVKTLGQNFLHDQNLARWIVNQAEIIPD
                     DYVVEIGPGLGALTRLALEKGANVLAIEKDTRLANFLRERLNHPRLEVLNTDALKFDP
                     GVLFSHRRVKLLGNLPYNISSPLLLKFLDYPSPISLWLCMLQKEMARRLSASPCTHDY
                     GSLTLRVQLRYRVQYLRSIPATVFFPKPEIDSALVRILPRDPLDLPARDDELLTKLIR
                     SGFSQRRKQVRKLLGTQVINWERAASILNFDSKARAENLSLLQWIGLANYIAPPAPPD
                     ALAIEDERLPVVDKNDRILRCARRSAVHGNNLRHRAVHILIFDQAGEVYLQQRSRWKD
                     RHPLR"
BASE COUNT          905 a         1165 c         1111 g          986 t
ORIGIN      
        1 actccttcgc ggcgcagctt cacatcgatc tcattcaaaa tcggaacggt ctgacgtgtg
       61 agcgcgagga acgttgggcc atcggttcgt tgcatcgcgg cgacaaatgc gccggccgtt
      121 tcttcgggat cggccggacg aatcacgtcg atgttaggca tcaaccgaac ggaactgacc
      181 gtctccaccg gttgatgggt cgggccgtct tcaccaacgc cgattgaatc gtgggtaaaa
      241 atgtaaagat tgggaagctt ggccagcgcg ccgagccgaa tggaagcgcg acaataatca
      301 gtgaacaccg tgaaggttgc gcccgacgcg cggaacagtc cgtgataaga gatgccgttg
      361 aggattgcgc acatgccgtg ctcacgaatg ccaaagcgga tgttgcgccc cccaggagtg
      421 tcgcgcgtga aatcgccgcc ctccttgatg taattcattg tcgatccgta caaatccgcg
      481 ctaccgctca ttagcaaagg catcgcttgg gcgatcggct gcaatacttc cccgccggct
      541 tttcgtgtcg ccagtttcgc atccttcgga aactcgggga tcttggataa aagatcagcg
      601 ggcacttccc tctcaatccc gtcatcaagc attttcgcca ggccggggtt cttctggcgc
      661 cactcgtcat aagtcttttc ccagcgatcg taatcggcga tcagcttctt tttatgctcg
      721 gcaaaataat cgtacacgtc ctgcgacacg aaataatgtt cctctggcag cccgagtgcc
      781 tttcgcgccg catccacata tttcgcgcct gcctctccat gcgccttgta cgtcccttcg
      841 acttccggga cccctttgcc tatgagcgtg tgggcaatga tgaactgcgg tttcccgttg
      901 tcgcgcgtct tcgccacgtt caaggcgtcg agaaactgtt gcatgtcatg gccatcgatc
      961 tcctgaacgt cgaatccgta tgcctccatg cgtttgcggg tgtcttcgct ctgcgtctct
     1021 ttcgccgccg cgtcgagcgt gacggcgttt gaatcgtaga tgaagatcaa attatcgagg
     1081 ccccaatggc cggccagcgc acacgcttcg gaagcgacgc cttcctgcat gtcaccatcg
     1141 ctacataaac agatcacatg ctggtcgaaa attttgtgtt catcggtgtt gaaacgcgcc
     1201 gccgccattt ttgttgcaac ggaaatgccc accgaattgc caactccctg cccaagcggc
     1261 cccgtagtgc attccactcc gggcgtgtcg ccaaattcag ggtgtcccgg cgtcttggaa
     1321 tgcaattgcc gaaaacgttt gatttccgac attggcaaat cgtagccact catgtgcagc
     1381 cacgcgtaga ggaacatgct gccgtgaccg cccgagagga cgaagatatc gcgattgatc
     1441 cagcgcggtt gatccggatt gtatttgagc gcgtagccaa agagcactgc gcccatctcg
     1501 gcgcagccga gcggcagccc gaggtgtccc gattgcgaag cctgcacggc gtccatgcac
     1561 aaaccgcgtg cttcattggc cgcccgagag agaatttcga catttagact catgatcact
     1621 ttataggcag agggcggacg gaatggaaat gttgaaaatt ccaactcgtg ctttttggca
     1681 ggatcgccgt atgaatgacg aagcccgaat gccgaatgac aaaagaaact cgaatgacga
     1741 agttcgaagc gacgccagag ctgacaaatt acttgggtat tcgtgcttag tcattccttc
     1801 gtcattcggc attcgtgctt cgtcatttct caaatgaatc cgccatccct cctccaaatt
     1861 gtcgagcggc tcgaacgtct tgcgggcagg ttgccctcca ggattcggaa agccgttctt
     1921 agcgagctta caccgctcaa gcaattattc ctccagcagc ggccgccgcg gtttcttttt
     1981 gcaggctcga gcgtaatgcc gctggggcag gtcattgctg ccctttttgc ctcgaaccat
     2041 ccaacaggta tacacaatgt tcctgtccga accggtcatt ggcacgatat cagcctaccc
     2101 gaccggggaa cgatctccat cctcgacgct cggggagccg atgcttctgc tgccattcaa
     2161 attcgggatg aactcaaata tcaacctgcc gatgttatct tttttctcga cgatggccaa
     2221 atcggaagcg agcccagcct gcatgagttg ctgagcgatc cagcctggaa taaaacgccg
     2281 gagccagacg cgaagataat tgaaatcatt cttccgtcgc caactgcggg gagcgaattc
     2341 gctccggcta atctggtttc ctttcgactg gaatcggtgc ctggatcaga tgccgcggga
     2401 accgtgcggt acttggaagt tttgagattg gtccctggca gtcatgatcg gggagtcgaa
     2461 tccacgaaag gcctcgaagt cgaactgcct gatgtggaat ccaagcggct gatgtcggtc
     2521 ctggcacgtg agttaccgaa tcaggcacgg atggaaatga tcaggatatc gcgcaatcgc
     2581 gaagcgcagc atgaagtggc acagcttttg gtgaaatctg cgactgcgat ctgtgcggca
     2641 attggcgcac agcccatccc gctcgctgac ttgccgattc tgaccagttt gcaagtcatg
     2701 atgatctccg gaatcatgta tgtaagcgga cgtgagcgga gcctgcgcgc cgcgacccaa
     2761 ttcattggcg ctcttggtgc aaacgtgggc gccgcgatgc ttttgcgcga aggcacacgc
     2821 gcgatcctaa aattttttcc cggctgggga aacgtcgtat gcgggctggt ggcaggctcg
     2881 ggaacctatg cgattgggcg cgccgcgacc gtctttttca tcgatggcgt ttcattgaag
     2941 gacgcgcgcc gaacgtatct ggccagccgc aagaagcggg cgcgcctggc tctgcccgct
     3001 cacgagtgaa aaccgtgcgt ttatttcgaa ccggtcgtat gcgcgaataa cttgtgaata
     3061 agattgctct ttccagcata ttttgttcac acaattttgt gaacaaatgc gttagtttct
     3121 tcgggccagc gaggcattcg tagctgtctt atgaagctct ccgagttaag gacgacgctt
     3181 cagcagatac gtgtttcgcc ggtcaagact cttggtcaaa actttctgca tgaccaaaat
     3241 ctcgcgcgct ggatcgttaa tcaggcggag ataattccgg acgattatgt cgtagaaatt
     3301 ggaccgggcc tgggcgctct caccaggctg gcgcttgaaa aaggcgcgaa tgttctcgca
     3361 atcgagaaag acacgcgcct cgcgaatttt ttgcgcgaac gtctcaacca tccgcgactc
     3421 gaagtactca atacagacgc gctgaagttt gatccgggtg ttcttttttc gcatcgtcga
     3481 gtgaaactgc tcggcaatct tccatacaac atctccagtc cgttgctctt gaagtttctc
     3541 gattatccta gccccatctc actgtggttg tgcatgttac aaaaggagat ggcgcggcgt
     3601 ctttcagcgt cgccatgcac tcacgattac ggctccctga ctttgcgcgt gcagttgcgt
     3661 tatcgcgtcc aatatctacg ctcgattccg gcaaccgttt tctttccaaa accggaaatc
     3721 gattcggcac tcgtgcgaat cttacctcgc gacccgctcg acctccccgc gcgcgacgat
     3781 gaactgctta cgaagttgat tcgaagtggc ttttctcaga gaagaaagca ggtaagaaaa
     3841 ctactcggca cgcaagtcat caattgggaa cgtgccgcgt cgattctgaa cttcgattcc
     3901 aaggcacgtg ccgaaaactt atcgctgctt cagtggattg gcctggctaa ttacatcgcg
     3961 cctcccgcgc ctccagatgc attagcaata gaagacgagc gtctgccagt ggtcgataag
     4021 aacgaccgaa ttttacgatg tgcgcggcgc tcggcggtgc atgggaataa cttgcgccat
     4081 cgtgccgtcc acatcctgat tttcgaccag gcaggcgaag tctatttgca gcaacgctcg
     4141 cgttggaaag atcgacatcc tctaaga
//
LOCUS       DASQUQ010000129         4353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_303999, whole genome shotgun sequence.
ACCESSION   DASQUQ010000129 DASQUQ010000000
VERSION     DASQUQ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303999"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VE486_03670"
     CDS             <1..373
                     /locus_tag="VE486_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38217.1"
                     /translation="AMNKASFRFYVSQRQRQISFDRAGGRLNYMLRNTNELLGQNGID
                     GVKTGRTSRAGDCLILSANREPELMKQGKMITVFPRHLIVVLLGSTNRFGEGAALVSR
                     GWQLYDQWAASGRLADPKKVL"
     gene            666..1742
                     /locus_tag="VE486_03675"
     CDS             666..1742
                     /locus_tag="VE486_03675"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HZA38218.1"
                     /translation="MKPRIGVLTSGGDCPGLNAVLRGVVLAAEKRGWEVVGFRDGFEG
                     LLPPGNHVILDRASINGIMALGGTIIGTTNRGHFVAKVGAGDKTIVPPKIIAGAKKIL
                     DDLRVKALIIVGGDGSMVTAQQLQEAGINCIGVPKTIDNDLEATATTFGFDSAVDVVM
                     DALDRVHTTATSHRRVMVVEVMGRHAGWIALHGGIAGGADIILIPEIPFDYDKIASVV
                     KTREAAGYLGTVVVVAEGARPKHGEQIKRDVEGGEFRLGGVGDLVAREIAKRTGKETR
                     CCVLGHLQRGGAPTTLDRILGTRFGVKAVQLANEGHFGSMVSYQNYQVRQVPIAEAVN
                     RLKLVPPNGEVVQTARDVDISFGD"
     gene            1991..2929
                     /locus_tag="VE486_03680"
     CDS             1991..2929
                     /locus_tag="VE486_03680"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HZA38219.1"
                     /translation="MMQNAAHAQSTDSVLMIRPARFYPNPETAADNAFQRDANCGSDA
                     LATAARKEFDAAVRTLRAAGINVHVFEDTAEPEKPDAVFPNNWISAHHDGRIALFPMY
                     SALRRRERRQDIVEELRKHYRVTEVIDYSAFEGEGCCLEGTGSLVLDHLNKIAYVSLS
                     NRSNRKVIQRFAEDFSYEPVTFTSIGSNGQPIYHTNVMMCIGTVFAMVGLEIIRNKAE
                     RQQVLARLEKAGKEIVELSADQIANFAGNAIELHNNGGEKLLVLSTRAARALTEEQHE
                     RLTRYARLVPLELPTIELGGGSARCMIATIHLPTPS"
     gene            3397..3894
                     /locus_tag="VE486_03685"
     CDS             3397..3894
                     /locus_tag="VE486_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZA38220.1"
                     /translation="MSSEIKKEIQLEIAHVLFIDIVGYSKLSINEQRAAVDELTRIVR
                     ATEQFQRAEASERLIKIATGDGMALVFYSSPEAPVRCAMELSRALKDHPRLGLRMGIH
                     SGPVSGVVDVTGRTNLAGAGINMAQRVMDCGDLATSCSPNMLPKIWQNTTIGDRFCTT
                     SVPVK"
     gene            3837..>4353
                     /locus_tag="VE486_03690"
     CDS             3837..>4353
                     /locus_tag="VE486_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38221.1"
                     /translation="MAEYDDWRPLLHDLGTCEVKHGIKLDVVNLNNAEVGRAELPKKL
                     RMQRRRSARMRGLIGAAALVVIAALVTGIIMFGRNQSPLTQVAPDKSIAVLPFENLSE
                     KKQNAYFTDGVQDEILTNLAKIADLKVISHTSVMQYKGDIAHNLREIGQQLGVTHLLE
                     GSVQRAGNRVRV"
BASE COUNT         1000 a         1177 c         1268 g          908 t
ORIGIN      
        1 cgccatgaac aaggcgagct tccggtttta tgtatcgcaa agacagcgtc agatttcatt
       61 cgatcgagcc gggggccggc tcaattacat gcttcgcaac acgaacgagc ttctggggca
      121 aaatggaatc gacggagtta aaaccggccg cacctcgcga gcaggggact gcttgatttt
      181 gtccgcaaat cgggagccag agctgatgaa acagggtaag atgataactg ttttcccgcg
      241 tcatttgatc gtggtgctgc tcggcagcac gaatcgattt ggcgagggcg cggcacttgt
      301 gagccgtggc tggcagctct acgatcagtg ggctgcttcc ggccgccttg ccgaccccaa
      361 gaaagtgctt taggagcctg ttcaaatact cagccatgcc gcgttgctgc cgatttttcg
      421 tctctccaag gcgcgaggag ggcgcatacc cgaagcggtc tgaaaccgac gagcaacgcc
      481 ggagaggcga aaaagcggcg ccaacccggg aaggcgagag cgctttaggc tgtctggcgg
      541 agttgctcac tcctcacagg tcgctttgga catgctcgtc gctcgcgcct tgccatccaa
      601 cccaaagcgc tctcgcgcga catggctgag tatttcaaca ggctcctagt cgcgccgctc
      661 cgaaaatgaa gccacgaatt ggtgtattga ccagtggtgg cgattgcccg ggactgaacg
      721 ctgttctccg cggagtggtg ctggcagcgg aaaaacgcgg ttgggaggtc gttggttttc
      781 gcgacggatt cgaaggactc cttccgcccg gcaatcatgt catactcgat cgggcgagca
      841 ttaatggaat catggccctg ggcggcacga ttatcgggac aactaatcgc ggtcattttg
      901 tcgcgaaagt cggcgccgga gataagacga ttgtgcctcc gaaaatcatt gccggcgcca
      961 aaaagatcct ggacgacctt cgggtaaagg cgttgatcat tgtgggcggc gacgggtcga
     1021 tggtaacggc ccagcaattg caggaagccg gcatcaattg catcggagtt ccgaagacaa
     1081 tcgacaacga tctcgaagca acagcgacga cgttcggatt cgattccgcg gtcgatgtag
     1141 tgatggacgc gcttgatcgg gtgcacacca cggcgacgag tcatcggcga gtcatggttg
     1201 tcgaagtgat ggggcgccat gcgggctgga tcgcgctgca cggcggcatc gccggcggcg
     1261 ccgatattat cctgattccg gaaatcccat tcgactacga caaaatcgcg agcgtggtca
     1321 aaacgcgcga ggcggctggt tacctgggta ctgtcgtagt cgttgccgaa ggcgcgcggc
     1381 caaaacatgg cgaacaaata aaacgcgacg tcgaaggtgg cgaatttcgt ctcggcggag
     1441 tcggcgacct cgtggcgcgt gagatcgcca aacgcaccgg caaagaaacg cgctgctgcg
     1501 ttctcgggca tctgcaacgc ggcggagcgc ccacgacgct cgatcgaatt ttgggcacgc
     1561 gcttcggcgt gaaagccgtc caactagcca acgaaggaca ttttggatcg atggtcagct
     1621 atcagaatta tcaggtgcgt caggtgccca tcgccgaagc cgttaatcgg cttaagcttg
     1681 ttccgcccaa cggcgaggtg gtccaaaccg cgcgcgacgt ggatatttca ttcggcgatt
     1741 aggatcttgt ctcacacaac gaacacaacg gacacgaagg agattctttg attcgttggg
     1801 atccttcgtg atcctttgtg tccttagtgc gatgaaatga aaatcgtcct cgcatgtcct
     1861 gtagcggcgg tctatgacct ccgattccaa tgaagatcgt cctcgcatat tctggcggac
     1921 tcgacacatc gataagttct ctgctggctc atgcgacatc ccggcaagtg gggtagtatg
     1981 gagctgctca atgatgcaaa atgctgcgca cgcgcaaagc accgattcgg tgctgatgat
     2041 ccgcccagcc cggttttatc ccaatcccga aacggccgca gataatgcgt ttcaacgtga
     2101 tgccaattgc ggctcagatg cgttggcgac cgcggcgagg aaagaattcg acgcagccgt
     2161 gcggacgctg cgcgcggccg ggataaacgt gcatgtcttt gaagacacag ctgagccgga
     2221 aaagccagac gcggttttcc ccaataactg gatctcggct catcacgatg ggcgcatcgc
     2281 ccttttcccg atgtattcag ccctgcggcg acgggagcgt cggcaggata tcgtcgagga
     2341 actacgtaag cactatcgcg tcaccgaggt gatcgattac tcggcgttcg agggtgaagg
     2401 ttgttgcctg gaaggcacag gcagtcttgt gctcgatcac ctgaataaaa ttgcctacgt
     2461 ctcgctctca aatcgctcca atcggaaagt gatccaacgc tttgccgagg atttcagcta
     2521 cgagccggtc actttcacca gcatcggctc caatggtcag ccgatctatc acaccaacgt
     2581 gatgatgtgc attggcacgg ttttcgcgat ggttggtctg gagataattc ggaacaaagc
     2641 cgagcggcaa caggtgctag cgcgcttgga gaaggccgga aaggagatcg tcgaattatc
     2701 cgcagatcag atcgcgaact tcgccggtaa tgccattgaa ctacacaaca acggtggaga
     2761 gaaattgctg gtgctttcca cccgcgccgc tcgagcattg actgaggaac aacacgaaag
     2821 attgacccgc tacgcgcggc tcgtgccgct tgaactccct acgattgaac ttggtggcgg
     2881 aagcgcgcgc tgcatgatcg cgacgattca cctgccgacg ccgtcgtagc ggcgctctgt
     2941 gaacgcattc gaatcacgcc ttcattccaa aatatggcga gctcgtttac aacggctttt
     3001 ggttctcgcc cgaacgcgag gcccttcacg cgctcgtcac agaaacgcag cgcgacgtta
     3061 ccggcgtcat gcggctcaaa ttttacaagg caacattatc gtcgccggcc acaaaagccc
     3121 ccaaagcctg tacgacctgc acattgccac gatgcaaggg gatgcgtctg cttacaacca
     3181 aagcgatgct acgggattta tacgattgaa cgcactcaga ctgaaactgc gagcggcaag
     3241 gaacggcgat ttccccggct cgccgtagcc ttggcggagg cggctaaatc gccgctcctt
     3301 gtttcccgat aaaacatgcc gcctcacgag gcgtgttcgt tagatcttta tctgctcgac
     3361 ggggcagcct gaaatgtgaa tgaatcgcat cggaacatgt cgtcggagat caagaaggag
     3421 atccagcttg agatcgcgca tgttttgttc atcgacattg tcggttattc aaaactctcg
     3481 attaacgagc aacgcgcagc tgtcgatgag ctgacacgaa tcgttcgtgc gactgagcag
     3541 tttcagagag ccgaagcctc ggaacgtttg atcaagatcg ccaccggcga tggtatggcg
     3601 ttggtattct actccagccc ggaagcaccg gttcgttgcg cgatggaatt aagccgcgcg
     3661 ctcaaagatc atccgcgctt aggcctccgc atggggatcc atagcgggcc ggtcagcgga
     3721 gttgtcgatg ttactggccg cactaatctg gctggcgcgg gcatcaatat ggcacaacgc
     3781 gtaatggact gtggcgatct ggccacatcc tgctctccaa acatgttgcc gaagatttgg
     3841 cagaatacga cgattggcga ccgcttttgc acgacctcgg tacctgtgaa gtaaaacacg
     3901 ggatcaagtt ggacgtagtc aatctcaaca atgctgaagt aggccgcgcc gagttgccaa
     3961 agaagcttcg gatgcagcgg cgacgcagcg cccgaatgcg agggctaatt ggagcagctg
     4021 cgctggtggt gatcgcggct cttgtcacgg gaatcattat gtttgggcga aaccaatcac
     4081 ctttgactca ggttgcgccc gacaaaagca tcgccgtgct cccatttgaa aatctgagcg
     4141 agaagaagca aaacgcctat ttcactgatg gcgtgcagga tgaaattctg accaatctgg
     4201 caaagatcgc ggacctgaaa gtcatcagcc acacctcggt aatgcaatat aagggcgata
     4261 ttgcgcacaa tctccgcgag atcggtcaac aactgggagt aacgcatcta ctggaaggta
     4321 gcgttcagcg cgccggcaat cgcgtgcggg tca
//
LOCUS       DASQUQ010000130         5353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_390683, whole genome shotgun sequence.
ACCESSION   DASQUQ010000130 DASQUQ010000000
VERSION     DASQUQ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390683"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VE486_03695"
     CDS             complement(<1..252)
                     /locus_tag="VE486_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38222.1"
                     /translation="MADAPDQPAKIQPWTWRVLLLVVVALLTIGCLYFAYRFSRDAPV
                     VYADPEEHFKYGSTGGERESGIPYWIWKVLPKLFPEYLPD"
     gene            complement(267..710)
                     /locus_tag="VE486_03700"
     CDS             complement(267..710)
                     /locus_tag="VE486_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38223.1"
                     /translation="MTVTVAEMLKMSQAQLDDLFTQSPAGEIPSGEAKGTAIVAPGTT
                     YTQNIANFINHFAWQGKIFDPGKGVLRNKILPFGINAIIAKVYKGPSWLDNKECIVLD
                     YSDTSLLAHWIRDEIREVAPRVYLGKVYWSKKRLIDFALEFPTPG"
     gene            complement(736..2889)
                     /locus_tag="VE486_03705"
     CDS             complement(736..2889)
                     /locus_tag="VE486_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38224.1"
                     /translation="MLPARGDILRLTRVGSCLGACALLTLTSCKKNQPIDEAQAAGLT
                     TADFPQITADIFKPMDGGIDLSPEEIMGRNTWNLWSGGNEHFWNQAAQDSFGLMDLLK
                     MLDNRKYPRGERFKILGAVNEPGFRAASKPDEYGLWLDEQTEPEPAGIDEKIYGKPSG
                     VLGYRLFPNPDFNDEARKKWDGNRFMNDPTYYNDNKLVRPYRVGVACGACHIAPFPNN
                     PPADPENPRWENLASAIGNQYINEGKVFACNVEKGGFFYEMLAAQPRGTSDTSRIATD
                     HINNPNAINAIFLLAERERIATPEKMAGGTLALPGEKEEMPVPHILKDGADSIGVAGA
                     TIRVYVNIGMFSEYWLTLHQRLIGLKPQKPFQIPYARDHSVFWLATEERLANVAAFFR
                     RLKPFHLADAPGGQAYVTTDEAVMTRGKEVFAESCAACHSSKRPPPNIDPRSGEGKAW
                     FRAAVMAPDFLESNFLSDDRRYPLTKIETNSARAFATNAKSGHVWDNFSSQTYKEISP
                     VDELDFFNPFDETRPIKFKPKDKNVGPGYYRTPSLVSLWSSAPFLHNNALGKFTGDPS
                     VAGRMEAFNDAVEKLLWPAKRLNKASIWRTQNECSLHLRKEFVPKWLRRFAGSDGYIR
                     IGPIPKGTPVNLIGNLPPDFGQAALGAKLLKALVKIHARNLSSEQATAELIKSVPELL
                     AANKCPDFIEDKGHYFGTDLADNDKRALIEYLKTL"
     gene            complement(3017..>5353)
                     /locus_tag="VE486_03710"
     CDS             complement(3017..>5353)
                     /locus_tag="VE486_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38225.1"
                     /translation="NLRSYSNYLSPRLGLFSADAWTLFATYIRNLLLTSLVMIPLLAA
                     ALTVPWFYEAVLMKNPIPYTSVPLWVGVAGVAMGVTYMGINLPCGGNKRGSQCSFLIF
                     CLLPLLIAAFSMTTYWAWFVAHGRTTPKWSFLGLTPPHQVVPFIYLGVLMHLLSWVTS
                     LLRAHGFRLKEFIAVIASGAIGGYLLWLGATKIFPQPLAVAELYGVFAVPLFLTLFFL
                     AVLTFAGISSRWTNDEDREWWGRAAGWFLAVVIGWMLISGLVIFGPLLLSRTISLLSA
                     GGIAGLITILAGRSSMLPANQEERDKAGPLALILSKAVTIAAFIFIVVLMILITRAMT
                     WTMLQIGEQLHFYWNLDAVSHVVGAKVEYLNVLLYTPISTLTWFALALLGFGVAMAHT
                     IDPNRFSLHAVYRDRLIRAYLGASNKQRDPNPFTGFDEKDNIRLRELWIEEKFGRRLL
                     PVINIALNLVGGSKLAWQERKAESFTASPLYCGSYELGYRKTDAPPGKRYGGEHGISL
                     GTATTISGAAASPNMGYHSSPLVTFILTLLNVRLGAWLGNPGKAGNATFHLGYPNFSV
                     GPIIAEAFGLTNNTSRYVYLSDGGHFENLGLYEMVLRRCHYIVASDAGEDPECSFADL
                     GEAVRKIRIDFGIPIEFGPMSIYPRSQIDALEGPGHNCAIGRIRYSVVDGPAAPDGII
                     VYVKPACYGDEPRDIYEYFKRSDTFPHESTADQFFSESQFESYRMLGAHTVEKFCTDC
                     GGDFRCFVRDILKTHLKPEKTPDWLAKLLDASENENKV"
BASE COUNT         1243 a         1478 c         1444 g         1188 t
ORIGIN      
        1 atccggcaga tattccggaa acagtttcgg cagcaccttc caaatccaat agggaatgcc
       61 cgattctcgc tcgccgccgg ttgagccgta tttgaaatgc tcttccgggt ccgcgtagac
      121 gacgggagcg tcccgactaa aacggtaggc aaaatataaa cagccaatgg tgaggagcgc
      181 gacgaccacc agcaatagaa cacgccacgt ccacggctgg atttttgcag gttgatctgg
      241 agcgtcggcc atggccgaga gggcgattat cccggcgttg ggaactcgag cgcgaaatcg
      301 atcaaccgtt ttttgctcca ataaactttt ccgagataaa cgcgcggcgc gacttcgcgg
      361 atttcatccc gaatccaatg ggccagcagc gaagtgtccg aataatcgag cacgatgcac
      421 tccttgttat cgagccagct gggaccttta tagactttcg caatgatcgc gttgatcccg
      481 aatgggagaa tcttgttgcg caaaacgcct ttgcccggat cgaaaatttt tccctgccac
      541 gcaaaatgat ttatgaaatt ggctatgttc tgcgtgtaag ttgttccggg tgcgacaatc
      601 gccgtacctt tggcctcgcc gctcgggatt tcgccggccg ggctctgcgt gaaaagatca
      661 tcgagctgcg cttgcgacat ttttaacatt tctgcaactg tgaccgtcat gttttctcct
      721 ttcgttggat gcgagctaga gcgttttcag atattcgatc agcgcacgtt tatcgttatc
      781 agccaaatcg gtgccgaaat agtgaccttt gtcctcgata aaatcggggc acttatttgc
      841 cgcaagaagt tctggaaccg atttgatcaa ttcggctgtg gcttgttccg atgaaaggtt
      901 cctcgcgtga attttcacca gggccttcag gagttttgcc cccaaggcag cttgtccgaa
      961 atcaggtggg aggttaccga ttaagttaac tggcgtgccc tttgggatcg gcccgatcct
     1021 gatatagcca tcgctaccgg cgaatctgcg aagccacttc gggacaaatt ctttccggag
     1081 gtgcaacgaa cactcgtttt gcgtccgcca aattgaagct ttgttgagcc gttttgccgg
     1141 ccagagcaat ttctccaccg catcgttgaa tgcttccatg cgcccggcca ctgagggatc
     1201 gccggtgaat tttccgagcg cgttattatg cagaaatggc gctgaactcc agaggctgac
     1261 cagagatgga gtccgataat aaccgggccc gacattttta tctttcggct tgaatttgat
     1321 cggccgcgtc tcatcgaacg gattgaaaaa atccagttcg tctaccggcg agatttcctt
     1381 gtaagtttgg gaggaaaagt tgtcccagac gtgaccggac ttcgcattgg tcgcaaacgc
     1441 gcgcgcggaa ttcgtttcga ttttggttaa tggataacgc cggtcgtcgg ataaaaaatt
     1501 gctctccaaa aaatcgggcg ccatcacggc cgcgcggaac caggcttttc cctccccgga
     1561 gcgcggatcg atattcggcg gcggtcgttt gctcgagtga catgccgcgc agctttcggc
     1621 gaaaacttct tttccgcgcg tcatgaccgc ctcatcggtg gtgacgtaag cctgcccgcc
     1681 gggagcgtcg gcgagatgaa acggttttag cctgcgaaaa aacgcggcga cgtttgcgag
     1741 acgctcctcg gtcgccagcc agaaaaccga atgatcgcgc gcatacggaa tctgaaacgg
     1801 cttttgtggt ttgagcccga tcagccgctg gtgaagcgtc agccaatact ctgaaaacat
     1861 gccaatgttg acataaacac ggatggttgc tccggctacg ccgatggaat ccgcgccatc
     1921 cttcaaaatg tgcggcaccg gcatctcctc tttttcgccg ggcaaggcca gcgttccgcc
     1981 cgccattttt tccggcgtcg cgattcgttc ccgctcagca agcaaaaaga tcgcattgat
     2041 cgcgtttgga ttgttgatgt gatcggtcgc gatccgcgaa gtgtcggaag ttccgcgggg
     2101 ttgcgcggcc agcatttcgt aaaagaatcc gcccttctcg acgttgcagg cgaagacttt
     2161 tccctcattg atgtactgat tgccgatcgc ggaggcgaga ttctcccagc gcgggttttc
     2221 cggatcggct ggcggattat tcggaaatgg cgcgatgtga caggcgccgc aagccacgcc
     2281 gacgcgatac ggacgaacga gtttgttatc gttgtaatag gtcggatcat tcatgaaacg
     2341 attgccgtcc cacttcttgc gcgcttcgtc gttaaaatcc ggatttggga acaagcggta
     2401 gccgaggaca ccggagggtt tgccataaat tttctcgtca atcccggctg gctcgggttc
     2461 ggtttgctca tcgagccaca aaccatattc gtccggtttg ctcgcagccc gaaacccagg
     2521 ttcgttcacc gcgcccaata tcttgaaacg ttcgcctctc ggatatttgc ggttatcgag
     2581 cattttcaaa aggtccatca acccgaagct atcttgcgcc gcctgattcc agaaatgctc
     2641 gttgcctccg ctccaaagat tccaggtgtt gcggcccatg atttcctcgg gcgaaagatc
     2701 gatgccgcca tccatcggct tgaaaatgtc tgccgtgatc tgcggaaaat cggcagttgt
     2761 taggccagct gcctgcgctt cgtcgattgg ctggtttttc ttgcagctcg tgagagtgag
     2821 cagcgcacat gcacccagac aacttccgac tcgagtgaga cgcagaatat cacctctggc
     2881 tggcagcaat cccttcaccg agccattatt gatatgtccc tcgtcagcct tgtggcaaga
     2941 aaattcctag gggttcaagg cagcgatcgc ggcgcgtccg gcagtcgcgc cctacgagaa
     3001 tcgatcactg gaagcatcag accttgtttt cattttccga cgcgtcgagc aatttcgcca
     3061 accaatcagg tgtcttctcc ggcttcagat gtgtcttcaa aatatcgcga acgaaacacc
     3121 gaaaatcgcc gccgcaatct gtgcaaaatt tctccacggt atgcgcaccg agcatgcgat
     3181 agctctcaaa ctgcgattcg gaaaaaaact ggtcggcggt gctctcgtgc gggaaggtgt
     3241 cgctcctctt aaagtattcg tagatgtcgc ggggttcatc gccataacac gccggtttga
     3301 cgtaaacgat aatgccatcg ggagcggcgg gaccgtccac cactgaatag cggatgcgac
     3361 cgatggcgca gttgtggccc gggccttcca gcgcgtcgat ctggctgcgc ggataaatac
     3421 tcatcggacc gaactcgatc gggattccaa aatcgatccg gattttgcgc acggcctcac
     3481 ccagatcggc gaaagaacat tccgggtcct cgcccgcgtc gctcgccacg atgtagtgac
     3541 atcgacggag caccatctcg taaaggccaa gattttcgaa atgcccgcca tcggacaaat
     3601 aaacgtaacg actggtgttg ttggttaagc caaacgcttc ggcaatgatc ggaccgacac
     3661 tgaagtttgg ataacccaga tgaaatgttg cgtttccggc ctttcccggg ttccctagcc
     3721 atgcgccgag gcggacattc aacaacgtga ggatgaacgt gaccagcggc gatgaatggt
     3781 agcccatgtt cggacttgct gcggcgcctg agatagtggt ggccgtccct aacgagatcc
     3841 cgtgctcgcc gccatatcgt tttcctggcg gcgcatccgt tttgcgataa ccaagctcgt
     3901 aggagccgca atagagtggc gacgccgtaa agctctccgc ctttcgctcc tgccaggcga
     3961 gtttgctacc tccgaccaga ttcagcgcga tatttataac cggtaataac cgccggccga
     4021 atttttcttc gatccaaagc tcacgtagcc ggatgttgtc cttttcgtcg aaaccggtga
     4081 acggattcgg atcacgttgt ttgtttgacg cgcccagata tgcgcgaatc aggcgatcgc
     4141 ggtaaacggc gtggagcgaa aaccggttcg gatcgatcgt atgagccatg gcgactccga
     4201 atccgaggag cgcgagcgcg aaccaagtca gggtcgagat cggcgtatac aaaaggacgt
     4261 tcagatattc caccttcgcg ccgacgacgt gagagacagc gtcaagattc cagtagaaat
     4321 gcagttgctc gccgatttgc aacatcgtcc aggtcatcgc gcgagtgatg agaatcatca
     4381 gcacgacaat gaaaatgaac gctgcgattg taacggcttt ggagagaatc aacgcaagcg
     4441 gtccggcttt gtcgcgctcc tcctgattcg ccggaagcat gctgctccgg ccggcaagaa
     4501 tcgtgatgag accggcgatc ccgccagcgc tcagaaggga aatcgttctg gaaagaagca
     4561 acggcccgaa aatgacgagt ccgctgatga gcatccatcc aatgacaact gccagaaacc
     4621 agcccgccgc gcgtccccac cattcgcgat cttcatcgtt cgtccagcga ctcgagattc
     4681 ccgcaaatgt caaaaccgcg aggaaaaaca gcgtcaaaaa aagtggcacc gcgaagacgc
     4741 catacaattc cgcaacggcc agaggttgcg gaaaaatttt cgttgcgccg agccaaagca
     4801 aataacctcc gatcgcgcct gatgcgatca cggcgatgaa ttcctttaac cgaaacccgt
     4861 gcgcgcgaag gagcgacgtc acccacgaga gaagatgcat cagcacgccc agatagataa
     4921 acggcacgac ctgatgcgga ggagtcagtc caagaaacga ccacttgggc gtcgttcggc
     4981 catgggccac gaaccaggcc cagtacgtcg tcatcgaaaa agccgcgatc agcagcggca
     5041 atagacaaaa aatcaaaaaa ctgcactgac tgccgcgctt gtttccgccg cacggcaaat
     5101 taattcccat gtaagtaacg cccattgcga cgccggccac gccgacccag agagggaccg
     5161 acgtgtacgg aattggattt ttcataagca ctgcttcgta aaaccacgga accgttaacg
     5221 ccgccgccag cagcggaatc attaccagcg aagtcaaaag cagattgcga atgtaagtgg
     5281 cgaacaacgt ccaggcgtcg gcggaaaaga ggcccagacg cgggctcagg taattgctgt
     5341 agctgcgcag att
//
LOCUS       DASQUQ010000131         7144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_567296, whole genome shotgun sequence.
ACCESSION   DASQUQ010000131 DASQUQ010000000
VERSION     DASQUQ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567296"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VE486_03715"
     CDS             complement(<1..348)
                     /locus_tag="VE486_03715"
                     /inference="COORDINATES: protein motif:HMM:NF021621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HZA38226.1"
                     /translation="MTPTSLIIVTDRGSLKAYRVNETPTRGPSLQLVQAFDLTDAHGR
                     LIDKVTDLAGRFPVTNGAGGHHGAASIAERTQLETETDRRIHKELADQIVKIVSSNGK
                     EGWSFAAPASIHAA"
     gene            complement(456..2684)
                     /gene="glgX"
                     /locus_tag="VE486_03720"
     CDS             complement(456..2684)
                     /gene="glgX"
                     /locus_tag="VE486_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZA38227.1"
                     /translation="MTSVKTWLGYPYPLGATWLGNGVNFALFSETATSVDLCLFDSID
                     ARQENVRIPVTEHTDQVWHVFLPEVRPGQLYGYRVSGPYDPEHGLRFNSSKLLLDPYA
                     KAIAGEVTWADEMFGYVVGGEDEDLTQDFRDDAWGVPKSVVIDNAFDWSGDKKLGVPL
                     HRSVIYEVHVKGFSKLWHDVPEELRGTYAALGTPAAIDYFNKLGVTALELLPVHAHID
                     DKTLIDRGLTNYWGYNTIGFFSPHAPYSSSGQFGEQVIEFKSMVRNLHAAGIEVILDV
                     VYNHTAEGNHLGPTLCFRGIDNPAYYRLQPDNPRFYLDFTGTGNTFNLLNSRALQLVM
                     DSLRYWVLEMHVDGFRFDLASTLARDHEGVNKLHAFFEIIHQDPVLSQVKLIAEPWDV
                     GEGGYQVGNFPVLWAEWNGKYRDTMRSFWKGDEGHIGELAYRLTGSPDLYQHSGRRPY
                     ASINFVTAHDGFTLTDLVSYNEKHNELNGDGNRDGDNNNQSWNCGVEGPTDDPKINAL
                     RERQRRNFLTTLFLSQGVPMLSGGDEWGRTKKGNNNTYCQDNDISWFNWERDEKQNQF
                     MEFTRKLIQFRRDHPAFRRPKFFQGRRIRGSEIRDVMWFNPGGSEMSEEDWTSPFVRC
                     LGMLLSGDTMDVFNFQGEPIPDNTFLLLINAHYEPIPFVLPGQEQIEWQLILDTMDPN
                     GFLMEPRKFASGDDVDLGGRAACLLQLVSGAQAQAREESWKKRRVEFPALSAEEERAR
                     GK"
     gene            2876..3400
                     /locus_tag="VE486_03725"
     CDS             2876..3400
                     /locus_tag="VE486_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZA38228.1"
                     /translation="MIRKITLLFVATSALALAVYFYAHHNWHPLPSGTTVDRIVVEKA
                     ARKLSIFREGKRLKTYRVALGRSPIGPKETEGDMKTPEGIYKIDSRNAQSSFHLALHI
                     SYPSADDNLRAAERGVSAGFDIMIHGIRNGIGWMGAFHRWKDWTAGCIALTDEEIEEL
                     WRLTPDGTTIEILP"
     gene            3604..4167
                     /locus_tag="VE486_03730"
     CDS             3604..4167
                     /locus_tag="VE486_03730"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZA38229.1"
                     /translation="MRLPNPLLLGAIYGLSELYLAFTRHSRTQAVSQDRRSLFVLWTV
                     IAVSLWLGIQMIWLFSRATLPYPRGLYFLGFVLFLAGLVLRWYSIGYLGRYFTIDVSI
                     SAGHKLVDSGPYRYVRHPTYAGALLAFVGLGFCFGNWLTVLFLTVPIIGAFLWRIRIE
                     EQALIEALGEDYRSYMRRTKRLIPGVY"
     gene            complement(4649..5068)
                     /locus_tag="VE486_03735"
     CDS             complement(4649..5068)
                     /locus_tag="VE486_03735"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZA38230.1"
                     /translation="MNPHPRLSIETATQLAVFLANRPGALARVCEALAKAEINIHALA
                     TSDTVDHSVVRMVVSDPTKALMLLGEAGVLALETEVLIIETASEPGVLAKIAERLAEA
                     EVNIEYAYLAGGRGAEKGLIVLRPSDVEKAQKVLRDL"
     gene            5205..6575
                     /locus_tag="VE486_03740"
     CDS             5205..6575
                     /locus_tag="VE486_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZA38231.1"
                     /translation="MHSNATVDPTMTNVAEARSLKASQPRRTIGFGTACSIVIANIIG
                     TGIFTSLGFQLADIQSGFALLMLWVVGGMAALCGALCYGELSAALPRSGGEYHFLSRI
                     YHPSLGFMAGFVSATVGFAAPIALAAMAFGKYFVGVFGVGSPVVMSFVIVWLVAAFHF
                     GNLKVGSAFQNLFTLVKLLLIGTLITAGFFVPAKQPINFLPTPGDAMSIFSAPFAIAL
                     VYVMYSYSGWNAAAYITGEIKQPEKNVPRSLLAGTGIVIVIYVLLNAIFLATTPAEEL
                     KGQLEVALVAGKHIFGSAGGRVAGAVICLGLISSISAMTWIGPRVSMSMGEDHWLLRL
                     LGRKNPHGIPTNAIILQLLIVNLLLLTRSFELVLIYTQFSLLICSLLAVLGVIVLRFT
                     HPKMMRPYRVWAYPLPPILFSIITIWMMFYLLRWHSRESLAGLGTALFGLLLYFCARK
                     RHGHST"
     gene            6710..>7144
                     /locus_tag="VE486_03745"
     CDS             6710..>7144
                     /locus_tag="VE486_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38232.1"
                     /translation="MITALIGAFGVSAQENPEIAPRALPVSAGPNDIARFLAGMPVPP
                     DSPLAPLTQDPAWQAHSAYFEKEFSKLSVRQLQKLHAWEDLYLPEATQPTPVAFYMFS
                     GPDFLYVDQFFPRASVYVLCGKESMGPPPDPLRIPNLSGALGN"
BASE COUNT         1464 a         2035 c         1941 g         1704 t
ORIGIN      
        1 cgccgcgtgg attgacgccg gcgcggcgaa cgaccagcct tcctttccgt tgctcgagac
       61 aattttcacg atttgatcgg cgagctcttt atgaatgcgg cggtcggtct cggtttcgag
      121 ttgcgtccgt tcagcgatgg aagccgcgcc gtggtgaccg cccgcaccat ttgttaccgg
      181 gaaacgaccg gccaggtccg taaccttatc gatcagccgc ccgtgggcat cggttaaatc
      241 aaacgcttgc accagttgca agctcggccc gcgagtgggg gtttcattca cccggtatgc
      301 tttcaaactg cctcgatcag tgacgatgat gagagatgtc ggtgtcatga ctaatcactg
      361 accaaaatag attggtcgcg cgccaaaagg cgcacatcac actctacaac tgattcgcat
      421 gtgacagcaa gagtgcgttg ccgaaatttt agttcctatt tcccgcgagc acgctcttct
      481 tctgcgctga gcgcgggaaa ctcgacgcga cgttttttcc acgattcctc gcgcgcctgg
      541 gcttgcgccc cacttacgag ctgaagcaaa caagcagcgc ggccgccaag atcgacatcg
      601 tcgccggagg cgaatttcct cggctccatc aaaaacccgt tgggatccat tgtgtcgaga
      661 atgagttgcc attcaatttg ttcctgtccc gggagaacga acgggatcgg ctcataatgc
      721 gcgttgatca gcaacaggaa tgtgttgtcg ggaattggtt caccctggaa attaaatacg
      781 tccatggtgt cgccgctcag cagcatgcca aggcaacgaa cgaacggcga tgtccagtct
      841 tcctcgctca tctcgctgcc gccgggatta aaccacatca catctcggat ttccgaacct
      901 cgaattcgcc ggccctggaa gaatttcggc cgccgaaaag caggatgatc ccttcggaac
      961 tgaatcagct ttcgcgtgaa ctccatgaat tgattctgtt tttcgtcgcg ttcccaattg
     1021 aaccaactga tgtcgttgtc ctggcagtaa gtgttgttgt tgcctttttt cgtccggccc
     1081 cattcgtcgc ccccggatag catgggaaca ccttgggaaa gaaaaagcgt cgtcagaaaa
     1141 ttccggcgct ggcgttcgcg cagcgcgttg atcttcggat cgtccgttgg tccctctacg
     1201 ccgcaattcc aggattgatt gttgttatcg ccgtcgcgat tcccgtcgcc attcagttca
     1261 ttgtgctttt cgttatagct gaccagatca gtcaacgtaa acccatcgtg ggcagttacg
     1321 aaattgatgc tcgcgtatgg gcggcgtccg ctgtgttgat aaagatcggg gctgccggtg
     1381 agccggtaag ccagttcgcc aatgtggccc tcgtccccct tccaaaagct ccgcatggta
     1441 tcgcgatatt tgccgttcca ttcggcccag agcacgggaa aatttccaac ctgatagccg
     1501 ccttcgccca cgtcccacgg ttccgcgatc aatttcacct gcgagagcac cgggtcctga
     1561 tgaatgattt caaagaaggc atgcagcttg ttcacccctt cgtgatcgcg tgccagggtg
     1621 gaagccagat caaaccgaaa tccgtccacg tgcatttcaa ggacccaata gcgcaagctg
     1681 tccatcacga gttggagcgc gcgggaattc agcaggttaa acgtgttgcc ggtgccggtg
     1741 aaatcgaggt agaaacgcgg attatccggc tgcaaccggt agtaagcggg attgtcgatc
     1801 ccgcggaagc agagtgtggg ccccagatga tttccctcgg cggtatgatt ataaacgacg
     1861 tcgagaatta cctcgatgcc cgctgcgtga aggttgcgca ccatcgactt gaactcgatg
     1921 acttgttcgc cgaattgccc gctgctcgag tacggcgcgt gcggggaaaa gaatccgatc
     1981 gtgttgtatc cccaataatt cgtcagaccg cgatcgatca acgttttgtc gtcgatgtgc
     2041 gcatgcacgg gcaacagctc gagtgcggtc acgccgagct tgttgaaata atcgatcgct
     2101 gccggcgtcc ccagggcggc gtaggtgccg cgcaattctt cgggcacatc atgccaaagt
     2161 ttgctgaagc ctttcacgtg cacctcgtag atcaccgagc ggtgcaatgg gacaccgagc
     2221 ttcttgtcgc cgctccaatc gaacgcgtta tcgattacga ccgatttagg caccccccac
     2281 gcgtcatcgc ggaaatcctg agtcaaatct tcgtcttcgc ccccgacgac ataaccgaac
     2341 atctcatccg cccaggtcac ttcccctgcg atggctttcg cgtagggatc gagaagaagt
     2401 ttcgaactgt taaatcgcaa tccgtgctcc ggatcgtacg ggccggaaac gcgatagccg
     2461 tacaattgcc ctggccgcac ttcgggcaaa aacacatgcc agacctgatc ggtgtgctcg
     2521 gtgacgggga tgcgcacgtt ttcctggcgc gcgtcgatac tatcgaacaa gcaaagatca
     2581 acggatgtgg ccgtttccga aaagagggca aagtttacgc cgttgcccag ccacgtcgct
     2641 cccagcggat agggataacc gagccaggtt tttaccgaag tcataagcgc gccgcgttcc
     2701 tatcgaacac gaaacgcgca ttggtaatcg cgaacgtgaa atcgcgcaag cagaacaagg
     2761 agcggcggat tcctaacgcc gcgccaccgg tggtttgcgc caccggctaa ttcccgagcg
     2821 acccttcggg tctggacact tgcgtttttg accaagccgt cgaaacctca ccgatgtgat
     2881 tcgcaaaatc actctgctgt ttgtcgccac ctctgcgctg gcgctggctg tttacttcta
     2941 cgcgcaccac aattggcatc ccctgccctc tggaacgacg gtcgaccgca ttgtcgtgga
     3001 aaaagcggca cgcaaattat caatctttcg cgagggcaaa cggctcaaga cctaccgcgt
     3061 tgcgctgggc cgcagcccaa tcggaccgaa ggaaacggaa ggcgacatga aaacgccgga
     3121 gggaatttac aagatcgaca gccggaatgc gcaaagcagc tttcatctcg cgctgcacat
     3181 ttcctatccg tcggcggatg acaatctgcg cgccgcagag cgcggcgtct ccgctggttt
     3241 tgacatcatg atccacggaa ttcgaaatgg aattggctgg atgggcgcgt ttcatcgttg
     3301 gaaggactgg accgcaggct gcatcgcact taccgacgaa gaaatcgaag aactctggcg
     3361 cctgacgccg gacggcacga cgattgagat tctcccataa gctcaaggag cggtgcgccc
     3421 tgtcgccacg ggcctgtggc ccgtctgcat ttcgcgcgga cccacccaga aacggcccac
     3481 agggccgtgg ctacaaaagc ctgcaaacgc tttagacgtc gcgtcgttgt ttacgcttgt
     3541 cgatgcaccg cccgtgagcg cagaatcctg ccgatgggac acgcgattcg gaattaattt
     3601 ctcatgcgac tgcctaatcc attgttgctc ggcgcgatct atggtctttc tgagctttat
     3661 cttgcgttca cccggcactc gcggacgcag gctgtgtccc aggatcgccg ctcacttttt
     3721 gtgctctgga cggttatcgc tgtgagcctt tggctcggga ttcaaatgat ctggcttttc
     3781 tcgagagcga cactcccata tccacgcgga ctttattttt tgggcttcgt cctgtttttg
     3841 gcagggctgg ttctgcgttg gtactcgatc ggttatctcg gccgctattt tacgattgat
     3901 gtgtcgatct cggccggaca caagctggtc gattccgggc cgtatcgcta tgttcggcat
     3961 ccaacctacg ctggtgcgct tctcgctttt gtcggattgg gattctgttt tggcaactgg
     4021 ctgacggttt tgtttctgac cgtgccgatc atcggcgcgt tcctctggcg cattcgaatc
     4081 gaggaacagg cgctcatcga agctctgggt gaagattacc ggtcctacat gcggcggacg
     4141 aagcggctta ttcccggagt gtattaggag gttcagtcga aacgacgaga agcggcaggc
     4201 gcttgccgct cggttggttt atcacctcgc gatccgtccc tttgtcattt cgagcgaagt
     4261 cgagaaatct cttcctgtta attccggacg atgccgcgtg attacaactt ctgggtttat
     4321 atcgtcacga atcgcaatca ctcggttctt tacattggcg tgacaaacag actttcccgg
     4381 cgagcctggg agcaccgcga aggaacggga gctgggtttg ccgccgatta tcgatgcaaa
     4441 aagctaattt actatgaaca ctatcgcgac gttcgcgatg taatcgctcg ggaatcgcag
     4501 ctaagagatg gtcgcgagcc aagaaggtcc agctaatcaa tcgattgaac ccaagttggc
     4561 ttgatcttgg cgccgacgtt ttgcaagaca gataaacagt aagagatttc tcgactccgc
     4621 ctgcgcctcg ctcgaaatga caagaagcct ataaatcgcg cagcactttc tgcgcttttt
     4681 cgacatcgga agggcgcaga acgatcagcc ctttttcagc tccccggcca ccagccagat
     4741 aagcatattc gatattaact tcggcctcgg cgaggcgctc ggcgattttc gcaagcacac
     4801 cgggctcgct tgcggtttcg attatgagca cttcggtttc cagcgcgagc accccggctt
     4861 cacccaaaag catgagcgct ttggtcggat cgcttacaac catgcgcacc accgaatgat
     4921 cgaccgtatc ggaggtcgcg agggcgtgga tgttaatttc ggctttagcc agcgcttcac
     4981 acacgcgcgc gagcgccccg ggacgatttg caagaaagac tgctagttgg gttgccgttt
     5041 cgatggagag cctgggatgt gggttcatgc cgctttcaaa gtgatgccgg gaatcgcttc
     5101 tgacaactca cgaaaatttg aacatgtgac tcggtgcccg tcttagatca ttaacgaact
     5161 gccagacgca gaaggccata ccggcgtgag ccaattcgct aatcatgcac tcgaatgcaa
     5221 ctgtcgatcc aacaatgacg aatgtcgccg aggctcgttc cctgaaagcg tcgcaaccgc
     5281 ggcgcacgat cggtttcggc acggcgtgtt ccatcgttat cgccaacatc atcggcaccg
     5341 gcattttcac gagtctcggt ttccagttgg cggacattca atccgggttc gcgctactca
     5401 tgctctgggt ggtcggcgga atggcggcgc tctgcggcgc gctttgctac ggcgaactga
     5461 gcgccgcgct gccgcgttcc ggaggcgaat atcattttct ctcgcggatt tatcatccct
     5521 ctctcgggtt catggcaggt tttgtttccg ctacggttgg tttcgccgcg cccatcgcac
     5581 tcgcggccat ggcttttgga aaatatttcg tcggagtctt cggcgtcggt tcaccggtcg
     5641 ttatgtcgtt tgtcattgtg tggctggttg cggcgtttca tttcgggaat ttgaaagtcg
     5701 gcagcgcgtt ccaaaatctt ttcacactcg tgaagttgct tctgatcggc acgctcatca
     5761 ccgctgggtt cttcgttccg gcgaagcagc caatcaactt tttgcctaca cccggcgatg
     5821 caatgtcgat ttttagcgcg ccattcgcaa tcgcgctggt ttacgtgatg tactcctact
     5881 cgggctggaa tgcggcggcc tatatcacgg gcgaaatcaa gcaaccggaa aaaaatgttc
     5941 cgcgctcgct gctggccggg acgggcattg tgattgtgat ctacgtcctg ctcaacgcca
     6001 tctttctcgc caccacgccg gccgaagaac tgaaaggtca actcgaagtg gcgttagtcg
     6061 cgggcaaaca cattttcggc tcggccggag ggcgcgtcgc cggcgccgtg atttgtctcg
     6121 gcctgatttc ttcaatcagc gcaatgacct ggatcggacc gcgggtcagc atgtcgatgg
     6181 gtgaagatca ttggctgctt cgattgcttg gccgcaagaa tcctcacggc attcccacga
     6241 atgccattat tctgcagttg ctgattgtaa acttgctctt gctgacacga agttttgagc
     6301 tggtgcttat ttacactcaa tttagtttgt tgatctgctc gctgctggct gtgctcggcg
     6361 tgatcgtgct ccgcttcacg catccgaaaa tgatgcggcc gtatcgagtc tgggcttatc
     6421 cgctgccgcc gattctcttc tcgataatca caatttggat gatgttttat ctgttgcgct
     6481 ggcatagcag agagtccctg gctggtctcg ggacggcgct tttcggtttg ctcctctatt
     6541 tttgcgcccg aaaacgccac ggtcactcca catgaaagca cattggaaca taggcctgtg
     6601 gcctgtgcgg ccagcgggtt tgaaacccgc tggcccggag ccagcggaca acatgtccgc
     6661 tgggcacaca ggcagaatgc ctatgttccg atctcacgcc atattgttgg tgatcacagc
     6721 cctgatagga gcattcggcg tttccgccca ggagaatccc gagattgcgc ctcgcgcact
     6781 cccggtaagc gctggaccca atgacattgc acgcttcctc gccggaatgc cggtgccgcc
     6841 agattctcca ctcgcgccgc tcactcagga tccagcctgg caggcgcatt cggcctactt
     6901 tgaaaaagag ttctcaaaac tcagtgtgcg ccaactccaa aaactgcatg cctgggaaga
     6961 cctctatctt ccggaagcga cacaaccaac tccggtcgcg ttttatatgt tcagcggtcc
     7021 cgatttcctt tatgtggatc aatttttccc gagggcctcg gtatatgttc tttgcgggaa
     7081 agaatcgatg gggccgccgc ccgatccgct ccgcatccca aatctctccg gcgcgctcgg
     7141 gaac
//
LOCUS       DASQUQ010000132         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_115553, whole genome shotgun sequence.
ACCESSION   DASQUQ010000132 DASQUQ010000000
VERSION     DASQUQ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115553"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VE486_03750"
     CDS             <1..691
                     /locus_tag="VE486_03750"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF023246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HZA38233.1"
                     /translation="QKFYRVAGGSTQLHGVASDIVLPSLSDLPEFGESALKRPLPYDG
                     VPKAKYTKWSDTHSLFIDQLKIRSEERIKNDPEFHYVMEDIDRLRHKLDDNRISLNED
                     FRKKEVADDKLRKELRSKERLARNQEEPRIYRLTLDTVDKPNLQLIMYPGKAAEAKKN
                     GVAPKVDPESAAPDADTDLIAGAGGDGTKEPAIDPARDEALSILADLVDLSRGPKTAS
                     ANVDKSPAQRP"
     gene            complement(704..1312)
                     /locus_tag="VE486_03755"
     CDS             complement(704..1312)
                     /locus_tag="VE486_03755"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZA38234.1"
                     /translation="MRIVFFLPVFICAAGSILAETSVEIDLEEQTAYLLKNGWPVLAS
                     PISSGRSGHLTKTGVFKIIEKERTHYSSMYGKIVDARGNTIVGDADADMQVPRGGKFV
                     PAPMHYFMRFHGADGLHAGYLPGYPASHGCVRMPQQYAIAFFNSVSVGTPVTVFGRTS
                     VGRYLSQSESRFPRGRNRLAGPRYDSRFDPRFAPPPPPFWWR"
     gene            complement(1414..2208)
                     /locus_tag="VE486_03760"
     CDS             complement(1414..2208)
                     /locus_tag="VE486_03760"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZA38235.1"
                     /translation="MRQYHDLLRLVLERGRPRSDRTGTGTLSIFGAQTRFDLREKFPL
                     LTTKKLHIKSIIYELLWFLRGDTNVRYLNEHGVTIWDEWADEHGDLGRVYGAQWRDWR
                     GVNDARVDQIENVIAQIKSNPQSRRLIVSAWNPVEIDKMALPACHVLFQFYVQEGELS
                     CQLYQRSADLFLGVPFNIASYSLLTLMIAQVCDLKPGEFVHTFGDLHLYRNHLEQARE
                     QLSRDCLPLPRVKLNSAIKDLCDFKFEDFELIGYDPHPSIKAPIAV"
     gene            complement(2309..>3096)
                     /locus_tag="VE486_03765"
     CDS             complement(2309..>3096)
                     /locus_tag="VE486_03765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA38236.1"
                     /translation="PGGTGGHGLDVVLRENAHRLRAILHGADYTRWNPASDVLLPAQY
                     DARRLRGKQACRNALLKEMKLAPAPHGPVFGMVTRVVEEKGFEVLMPLLDRLLWDDVR
                     LIILGEGDTAYETALAIAARKFPDKFAYQKDYDEKLAHLIEAGMDISLIPSRFEPAGL
                     RAMYDLRYGALPAARATGGIQEIIEDYDPTTERGYGFLCYEYSSEAFWDTIKRARETF
                     RNRETWVKLMKRAMARNFSWNASARRYEELYRELVGATDEVAA"
BASE COUNT          734 a          882 c          824 g          656 t
ORIGIN      
        1 tcagaagttc tatcgcgtcg ctggcggctc gacccagctt cacggggtgg cctccgatat
       61 tgtgctgccg agcttgagtg acctgcccga atttggtgag agcgcgttga aaaggccgct
      121 gccctacgac ggagtgccaa aagctaaata tacgaagtgg tccgacacgc attccctgtt
      181 tatcgatcaa ttgaagatac gctcagaaga gcgcattaag aacgatcctg aatttcacta
      241 cgtgatggag gacatcgacc gcttgcgtca caagctggac gataatcgca tttcactgaa
      301 cgaagatttc cggaagaagg aagttgcgga cgataagctg cgcaaagaat tgcgttcgaa
      361 agagcgtctg gcccggaacc aggaagaacc gcgcatctat cgccttaccc tcgacacggt
      421 ggacaagccg aatctccaat tgatcatgta tcccggaaaa gcggccgagg cgaagaagaa
      481 cggagttgcg ccaaaggttg accctgaatc cgccgcgccg gatgcagaca cggatttgat
      541 cgctggtgca ggcggagacg gcacgaaaga accagccatc gatcccgctc gggatgaggc
      601 attgagcatc ctggccgatc ttgtcgatct ttcgcgcgga cccaagacgg ccagcgcgaa
      661 cgtcgataag tcgcccgccc agcgaccgta atatctaatt aattcatcgc caccaaaatg
      721 gtggcggcgg cggcgcaaag cgcggatcaa aacgtgaatc gtagcggggg cctgccaatc
      781 gattgcgacc tcttggaaac ctggattccg attgactcaa ataacgcccg actgatgttc
      841 tgccaaaaac agtgactggg gttccgacac tgacggaatt gaaaaacgca attgcgtatt
      901 gctggggcat gcggacacag ccgtgcgaag cagggtagcc cggcagatat ccggcatgca
      961 gcccgtccgc tccgtggaat cgcataaagt aatgcatcgg cgccgggaca aattttcctc
     1021 cccgagggac ctgcatgtcc gcatccgcgt cacccacgat ggtgtttccg cgcgcatcga
     1081 caattttgcc atacatgctg gagtaatgag ttcgttcctt ctcgatgatc ttgaacacac
     1141 cggttttagt gagatgccct gaacgtccgc tcgagatcgg cgatgccagc accggccacc
     1201 cgtttttgag aagataagcg gtctgttcct ccaaatcgat ttcgactgat gtttcggcca
     1261 gaattgaccc ggctgcgcag ataaaaaccg gcagaaaaaa gacgattctc acagaaagtt
     1321 caacccacgc ggcgcgcgaa agtcgcgagg cgctgggtct gctgttccgg ggagcacagg
     1381 ctggcagctt gtgctcccca gcactcccag cgtttacact gcaatgggag ccttaatcga
     1441 tggatgcggg tcgtagccga tgagttcgaa atcttcaaat ttgaagtcgc aaaggtcttt
     1501 gatcgcagag ttcagtttca cccgcggcaa cggtagacag tcgcgagaaa gttgttcgcg
     1561 cgcttgctcg agatggttcc ggtacagatg caaatcgccg aatgtgtgaa cgaattcccc
     1621 aggctttaaa tcgcatacct gagcgatcat taatgtgagg agcgaatatg aggcgatgtt
     1681 aaatgggacg ccgagaaaga gatccgcgct ccgctgataa agctggcaac tcaattcgcc
     1741 ctcttggaca taaaattgaa agagcacatg acaggccggc aacgccattt tgtcgatctc
     1801 aaccggattc catgcgctca cgatcaggcg ccggctctgc ggattcgatt taatttgcgc
     1861 gatcacgttt tcgatctgat ccacacgcgc atcgttcacg ccgcgccaat cgcgccattg
     1921 cgctccgtaa acccggccca gatcgccatg ttcgtcagcc cattcgtccc aaatagtgac
     1981 gccgtgttcg ttgagataac gaacattggt gtcgccgcgt aaaaaccaaa gcagctcgta
     2041 aataatcgat ttgatgtgga gctttttcgt cgtcaataac ggaaatttct ctcgcaaatc
     2101 gaaccgcgtc tgtgcgccga agattgataa tgtgccggtg ccggtgcgat cgctgcgcgg
     2161 ccggcctcgc tccagaacaa gccggaggag atcatgatat tggcgcacgc ttgaaattat
     2221 cccgcccttg gcgggatgcc attcgcaaaa acaaaaaaaa cttttggatc gcctcgcgct
     2281 gctgccgaaa cgcttacact cgccgcagct acgccgccac ttcgtcggtt gccccaacga
     2341 gttctcgata aagctcttcg tagcgccgtg ccgacgcatt ccacgaaaaa ttgcgcgcca
     2401 ttgcccgttt catcagtttc acccacgttt cccgattgcg aaaagtctct cgtgcgcgtt
     2461 tgatcgtatc ccagaaagcc tcgcttgaat attcataaca gagaaaacca taaccccgct
     2521 ccgtggtagg atcgtaatcc tcgatgattt cctggatgcc gccggtagct cgcgccgccg
     2581 ggagagcgcc atatcgcagg tcgtacatgg ccctcaaacc agccggttcg aagcgcgacg
     2641 gaatcaggct gatatccatg cctgcctcaa tcagatgcgc gagtttttcg tcgtaatcct
     2701 tttgatacgc gaacttgtcc ggaaattttc tcgcggcgat ggcgagcgcg gtttcatagg
     2761 ccgtgtcgcc ttcgcccaga ataatcagac ggacgtcatc ccacagtagc cgatcgagaa
     2821 gcggcataag aacctcgaag cccttttcct caaccacccg agtcaccatg ccgaaaactg
     2881 ggccgtgcgg cgcaggcgcg agtttcattt ctttcaacaa ggcgttacga caggcctgtt
     2941 ttccccgcag ccttctcgcg tcatactgcg ccgggagcaa aacatcggag gccgggttcc
     3001 atcgtgtgta atccgccccg tgcaagattg ccctcaaccg atgcgcattt tcgcgcagca
     3061 ccacgtcaag accgtgaccg cccgtcccgc cagggc
//
LOCUS       DASQUQ010000133         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_673616, whole genome shotgun sequence.
ACCESSION   DASQUQ010000133 DASQUQ010000000
VERSION     DASQUQ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673616"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /gene="gatB"
                     /locus_tag="VE486_03770"
     CDS             <1..919
                     /gene="gatB"
                     /locus_tag="VE486_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZA38237.1"
                     /translation="SLKQILIYGAVSDADMEKGQLRCDCNVSVRPEKQAELGAKIEIK
                     NMNSISGVRRALDYEIKRQIGILESGGKLEQETRGWDDAAAETFLMRTKEFAHDYRYF
                     PDPDLVPVKTEVLVADVRPRLPELPKAKRARFIDQYQVSPYDAGVLANDLDLARYFET
                     AAKGAKKPKNVANWILNDLQNALSNAGKTIDDCPIPPEALDELVNLIDTGKISGKQAK
                     EVFAEMFASGKGAAAIVKEKGIEQLSDASAIEALCEQVIAANPKPAADYKAGNVASLN
                     FLKGQVMKLSKGKANPQLAGEILERKLRS"
     gene            1019..2128
                     /locus_tag="VE486_03775"
     CDS             1019..2128
                     /locus_tag="VE486_03775"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZA38238.1"
                     /translation="MKILIAPDKFKGALSARETAESISLGLRDVLRDAKIDILPMADG
                     GEGTAEVICNALGGSWLKCNAHDPLGREIDARYAWIAKRKLAAMEMSEAAGMRRLSES
                     ERDPLRASTFGVGEMILEAAQREARQIIIGLGGSATNDGGFGMARALGFRFFEQEQET
                     ELCGAVSELVKLHRIEKPKDLALPKIIAAVDVRNPLLGANGATRVFGPQKGATDEKIN
                     ILERALAKLADVVSKEFGVDYRNEPGTGAAGGLGFGLTSFCGAKIQPGFDVVAEAADL
                     ESKMKDVDVVITGEGSLDRQTLEGKTPAGVARLARKLRKRVVAMVGRASQDRDVGELF
                     DEVYELAQPKMSEKENMARAAELLRERAEELAKTL"
     gene            complement(2219..2713)
                     /locus_tag="VE486_03780"
     CDS             complement(2219..2713)
                     /locus_tag="VE486_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZA38239.1"
                     /translation="MKTILGFAIAMLLAVVFPQVRLSAEDTQSADMRLYPPTTIEWKE
                     GPASLPPGAKMALLEGDPTKEGPFVMRLQFPDGYHVPAHTHPKTERVTIISGTGYLAA
                     RDTLDRSSAKSLPAGSFGYWPAGMKHAAWFEGETVIQLHGVGPWQINYVNPADDPRKA
                     KKSP"
     gene            complement(2754..>2973)
                     /locus_tag="VE486_03785"
     CDS             complement(2754..>2973)
                     /locus_tag="VE486_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZA38240.1"
                     /translation="MGAIILDDVEVGARSIVGANALVTIGMKIPPGSLVLGSPAKIRR
                     QLTLDEQKDIARWAWSYVETAKQFRELC"
BASE COUNT          706 a          775 c          905 g          587 t
ORIGIN      
        1 ttcgctcaaa caaatcctga tctatggagc cgtgagcgat gccgatatgg agaaagggca
       61 gctccgatgc gactgcaatg tttccgtccg acctgaaaaa caagccgaac tcggagccaa
      121 gatcgagatc aaaaacatga attcgatcag cggcgtccgg cgcgcgcttg attacgagat
      181 taagcggcaa atcgggattc tggaaagcgg cgggaaactc gaacaggaaa ctcgcggctg
      241 ggatgatgca gccgccgaaa cctttctgat gcgaacaaaa gaattcgcgc acgattatcg
      301 ctattttccc gatcccgatc tcgtcccggt gaaaaccgag gtgctggtcg ccgatgtgcg
      361 cccgcgcctt ccggaattac caaaagcaaa gcgcgcgcgc ttcatcgacc aatatcaagt
      421 gagcccgtac gacgcaggcg tgctggcgaa cgaccttgat cttgcgcgat atttcgagac
      481 ggctgcgaaa ggcgcgaaga agccgaagaa tgtcgccaac tggattctga acgatttgca
      541 gaacgcgtta agcaacgccg gcaaaaccat cgatgattgt ccgattccac cggaagcgct
      601 cgatgagctg gtcaatttaa tcgacaccgg aaaaatcagc ggtaaacagg caaaagaagt
      661 cttcgcggaa atgttcgcga gcggcaaagg cgcggctgcg atcgtgaagg aaaagggaat
      721 cgaacaactg agcgacgcga gcgcgattga agcgctctgc gaacaagtaa tcgccgccaa
      781 tcctaagccc gccgctgatt ataaggcggg caatgttgcc tcacttaatt ttctcaaagg
      841 ccaggtaatg aaattatcca aggggaaggc gaatccgcaa ctagctggtg aaattttgga
      901 acggaagttg agatcgtagc acagaggtcg tcgtctgtgg gaccggcggg cttccagcct
      961 gcttgatccg gctggcgaga cgcccaccgg ccccacaagc aagatgcact gtgccactat
     1021 gaaaatatta atcgcgcccg ataaattcaa aggggcgctc agcgctcgcg aaacggcaga
     1081 gagcatctcg ctcggactgc gcgacgtttt gcgtgacgcg aagatcgaca tcctgccgat
     1141 ggcggatggc ggcgaaggaa ctgcggaagt tatttgcaat gcgcttggcg gttcatggtt
     1201 gaaatgcaac gcgcacgatc cactcggccg cgagatcgat gcgcgttacg cctggatcgc
     1261 caagcgaaaa cttgccgcta tggaaatgag tgaagcggcg ggaatgcggc gactttccga
     1321 aagtgaacgg gacccgcttc gcgcgagcac attcggcgtc ggtgaaatga ttctggaggc
     1381 ggcgcagcgc gaggcgcgcc agatcattat cgggcttggc ggcagcgcaa caaatgacgg
     1441 cgggtttgga atggcgcgcg ctcttggctt ccgattcttt gagcaggagc aggagactga
     1501 attatgtggg gcggtcagtg agctggttaa gctgcaccga attgagaagc cgaaggattt
     1561 ggcgctgccg aaaataattg cagccgtcga tgtgcggaat cctttgctcg gtgcaaatgg
     1621 cgcgacgcga gtgttcggtc cgcagaaggg cgcgactgac gagaagatca acatcctgga
     1681 acgcgcgctc gccaaactcg ctgacgttgt ttcaaaagaa ttcggcgttg attatcgtaa
     1741 cgaacctgga acaggcgccg ccggcggact cggattcggt ctcacgagtt tttgcggcgc
     1801 gaaaattcag ccgggcttcg acgtcgtggc ggaagcagcc gatctagaat cgaaaatgaa
     1861 agacgtcgat gttgtcatca ctggcgaagg gagtttggat cgacagacgc tcgaggggaa
     1921 aacgcccgcc ggcgtggcgc ggctcgcacg aaaacttcgg aagcgcgttg tcgcaatggt
     1981 cggacgcgcc agccaagatc gcgacgttgg tgagttgttc gatgaagttt acgaactggc
     2041 gcagccgaaa atgagcgaaa aggaaaacat ggcgcgcgca gcagaattat tgcgcgaacg
     2101 cgcagaggaa ttagcgaaga cgctgtagcg gcggtctatg accgtcgcgc attagtccaa
     2161 atctatcggt ctatcggcaa aaagtgcggc ggttaccgac gccgctacag ccggacggtc
     2221 aaggtgactt cttcgccttt cgcgggtcgt cggcgggatt gacgtaattg atctgccacg
     2281 ggcccacacc gtggagctga atcaccgtct ctccttcgaa ccaggcagcg tgcttcatgc
     2341 cggcaggcca gtaaccgaac gagccagcag gaaggctttt cgcactgctg cgatcgagcg
     2401 tgtcgcgagc cgcgaggtaa cccgtccccg aaatgatagt tacacgctcc gtttttggat
     2461 gggtatgtgc cggcacgtga tagccgtcag gaaactgaag gcgcatcacg aacgggcctt
     2521 ctttcgttgg atcgccttcc agtagggcca tcttcgcccc aggtggcaaa gacgccggtc
     2581 cctccttcca ttcgatagtt gtcggagggt aaaggcgcat gtctgcggat tgcgtgtctt
     2641 cggcggataa ccgcacttgg ggaaagacca cggctaaaag catagctatt gcgaaaccaa
     2701 gaatagtttt catgcttcga gtattgtcgc ccgatttcgc ggtggcaagt cgtttagcag
     2761 agttcgcgaa actgctttgc tgtttcgacg tagctccagg cccatcgcgc gatgtctttc
     2821 tgttcgtcga gggtaagttg ccgccggatt ttggcgggtg accccagcac gagcgagccc
     2881 ggaggaattt tcatcccgat cgtgaccagc gcgtttgccc cgacgatgga tcgtgcgcct
     2941 acctctacgt cgtcgagaat gatagcgccc atc
//
LOCUS       DASQUQ010000134         7594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_205720, whole genome shotgun sequence.
ACCESSION   DASQUQ010000134 DASQUQ010000000
VERSION     DASQUQ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205720"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..453)
                     /locus_tag="VE486_03790"
     CDS             complement(97..453)
                     /locus_tag="VE486_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38241.1"
                     /translation="MQREDDQQLWDLLGRTVEPKLSPFFARNVLREVRQEASPFGRAQ
                     NWFSLRRLGAASAVAAVVIGMAIATHAPRWSQKSPEREPDVVAKIDPQDFDVVADLDE
                     LLAWDEDSLWNEKPTL"
     gene            complement(526..2157)
                     /gene="rpoD"
                     /locus_tag="VE486_03795"
     CDS             complement(526..2157)
                     /gene="rpoD"
                     /locus_tag="VE486_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZA38242.1"
                     /translation="MARLHSPIEIQSRIRELIKLAKEQGYLTFDDLNETLPEGITDAD
                     ELDLILTRLRRMEIDIIEASEVDRYKDGKKDAEEEEEEEEKTGPKLDILDDPVRMYLR
                     QMGQVPLLTREQEVEISKRIEEAENMVQKHINRFGFIARAHLDLARKLAEGKERFDRV
                     ILDKKIESRERYMKKLPHLCQQVERLSASISEQYCQLSRNSSQKQSKKLKSLQRAVAS
                     LQKIYPSFYFKQKVTEEFAHLTDEACHTALYLQTEMAKRPSDRKKRLQLREKIRELES
                     SLWLSLPEYAGHYQALKGWMRQALLAKTEMVEANLRLVISIAKKYTNRGLSFLDLIQE
                     GNMGLMKAVEKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLMR
                     VQKQLVQEYGREPTPDEVAEEVLLPVDRVRAVLKMAQQPISLQSPVGESEETNFGDFI
                     EDRGAENPSDMTAIVLLKEKIKDVLETLTDRERQVLEQRFGLVDGYSRTLEEVGRQFQ
                     VTRERIRQIEAKALRKMRHPTRIRQLEGFLEASGA"
     gene            complement(2432..4258)
                     /gene="dnaG"
                     /locus_tag="VE486_03800"
     CDS             complement(2432..4258)
                     /gene="dnaG"
                     /locus_tag="VE486_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZA38243.1"
                     /translation="MAIPSQTIEQVAAANDIVEVIGSYFPLKRAGANFKALCPFHQEK
                     TPSFHVSPQRQTFHCFGCGAGGSVFRFVMDYEHIDFPSAVRKLAARVGIPVIEERGPG
                     DAENRQHEARRTLLKVHAEAAEWFHENLLKKKFAEPAREYLKQRGITAEIAKRWRLGY
                     APDEWDAFGSWARGQGFGPRELLESGLVKTKDKADQTSKIEHQTSYDRFRDRIMFPIC
                     NDLGEVIAFSGRVLKEKEGAAKYLNSPETPLFRKGSVLFGLDKTKRALIEANCVIVCE
                     GQLDLISLFEAGITNVVAPQGTAFTETQARTLKRLVDEVVLCFDSDAAGAKAAERSLD
                     ALLENDLIVRVAEIPPGDDPDSLVRREGKEEFEKRIAAARDFFDYWIEHEVAEVDLAS
                     LGAKMQLARSLAETVSRVHDPLMRGEVASKVSARLGVPVQDFQTLFPKQSRSSGIRAD
                     ESRPATSGTPPRHDMAMLCLLALRDEAARDFLLQQNWQQVLAQVPDAEILVRILESDL
                     RPNDAASLNAFMATLSAPDEGLVSSWLLQKVPPNAGTMVEKWWLGIRQGALRRQLDVA
                     KNRIRLPQLSTGELVNLQKEILDLVKELRELPKLSSASRIDG"
     gene            4397..5809
                     /locus_tag="VE486_03805"
     CDS             4397..5809
                     /locus_tag="VE486_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38244.1"
                     /translation="MSKIFVSALTIWAALFLLVPASVHARDAINKGDVVVAPLRGEVG
                     PSLLAFLRRAVKTAESNEAAAIIFEMNTHGGRLDAAADIVSALNQTKIPTYTFINTNA
                     GSAGAIIAIATQHIFMAPVSAIGAAAPILPTGEDLPTTAREKTISYWSALIRGSAIKN
                     GHNPDVAEAFMNKDKEVKIGDRVIHRPGAVLTLNAQEATERINGKPLLAEGMADSIAD
                     LVKKAGLKGNVVAIEPTGFEQIALWITALAPLLLLLGILGAYLEFKVPGVGWAGIISA
                     ICFALFFLGHYVAGLAGWEVVALFVLGIVLVLIEILFFAHSTIVFGVLGVFLMIGSLL
                     WTMIDRYPQQPFLPTGEMLAIPLLNMFIAIVGSLIVIALLARYLPRTSLYRRFALIDS
                     NPPGPSLAGAPRQFATALSLTPGMQGTAVTVLRPSGKARFADHVVDVVTEGEFIAPET
                     PVTVLQTDGMRVVVKSASQI"
     gene            complement(5941..6246)
                     /locus_tag="VE486_03810"
     CDS             complement(5941..6246)
                     /locus_tag="VE486_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38245.1"
                     /translation="MVKRTFRDHRAALILIAINTLIVVEEFARQHLHWGKYPGLGWYW
                     TAMLSLPSSLALYLLRWPWSSEILSLVALLFLGAFQWGIVGGTLDRRSRMQQSSSNP"
     gene            complement(6765..7124)
                     /locus_tag="VE486_03815"
     CDS             complement(6765..7124)
                     /locus_tag="VE486_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38246.1"
                     /translation="MLDDYIDWYRAGMAIAYVCIDIYSVGLWWFAVRRTHLPFFRLWL
                     VVGIAYLLVSVLHVIITLSEEKLKWDVFGAHAYANFAHTEYVVRPFISVFGLIAATLM
                     VRWAIRVNAREHEATKV"
     gene            complement(7188..7388)
                     /gene="thiS"
                     /locus_tag="VE486_03820"
     CDS             complement(7188..7388)
                     /gene="thiS"
                     /locus_tag="VE486_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZA38247.1"
                     /translation="MTISLNGETVDARGAETIAQLVERHQLPPQSILVEHNGLAVHRH
                     EWPTRPLAEGDRVEFIRVVAGG"
BASE COUNT         1624 a         2175 c         2056 g         1739 t
ORIGIN      
        1 gggggggcgg ccccccgtgc cgcccgttag accgctgcgg ctgcgcctgt gccaaggcac
       61 agaacgaagc gaaaatcgac aaggtggtga ttacttttac aaagttggct tttcgttcca
      121 caggctatct tcatcccagg cgagcaattc atccaggtcg gcgacgacat cgaagtcttg
      181 cggatcgatc ttcgccacca catcgggttc ccgctcagga gatttttgcg accacctggg
      241 ggcgtgcgtg gcgattgcca ttcctatcac cacagctgcc acggcagatg ctgcgccgag
      301 tctccgcaag ctgaaccagt tttgcgctcg cccaaaggga ctcgcttcct gacggacctc
      361 tctcaataca tttcgcgcga agaacggcga aagcttgggt tcgacggtcc ggccgagcaa
      421 gtcccataat tgttgatcat cttcgcgttg catagtctcc gttgaattga actacttgca
      481 ccgttggaag ttgcgctcag acagaacgct aaagaatcga tttttttacg ctccggaagc
      541 ctcgaggaat ccttcgagct gccgaatacg ggtggggtgg cgcatcttgc gcaaagcctt
      601 ggcttcgatc tgacgaatgc gctcacgagt cacctggaac tgccggccta cttcttcaag
      661 cgttcggctg tagccatcga ccagcccaaa acgctgctcg agcacctgcc gttcgcgatc
      721 cgtcagggtt tcgaggacat ctttgatttt ctccttcaac aggacaatgg cagtcatgtc
      781 gctggggttt tcggcgcctc tatcctcgat aaagtcgcca aaattcgttt cttcactttc
      841 gccgacgggc gattgaagcg agatcggctg ctgagccatt ttcaagaccg cgcgaacgcg
      901 atcgaccggc aacagcacct cttccgccac ctcatccggc gtaggttcgc ggccgtattc
      961 ctgcacgagt tgtttttgca cgcgcatgag cttgttgatc gtctcgatca tgtgcacagg
     1021 aattcgaata gtgcgcgcct ggtcggcgat cgagcgggta atcgcctgcc gaatccacca
     1081 cgttgcgtaa gtcgaaaact tgtagccgcg ccgatattcg aacttctcga ccgccttcat
     1141 tagccccatg tttccttcct gaatcaggtc gagaaaagac agaccgcgat ttgtgtattt
     1201 ctttgcgatc gagatgacca ggcgcaggtt cgcttccacc atttcggtct tggccagtaa
     1261 tgcctggcgc atccaacctt tcagggcctg gtagtgtccg gcgtattccg gaagcgaaag
     1321 ccacaggctg ctttccaatt cgcggatttt ctcccggagc tgcaaacgtt tttttcggtc
     1381 actgggacgt tttgccatct ccgtctgaag atagagcgcc gtatggcagg cctcgtctgt
     1441 cagatgcgca aattcctccg tgactttctg cttgaagtaa aaagagggat aaatcttttg
     1501 cagcgaagct accgctcttt gcagagactt gagtttcttc gattgttttt gtgaagaatt
     1561 gcgcgaaagc tggcaatact gctcgctgat cgaggcgctc aatcgctcca cctgttggca
     1621 caggtgtgga agctttttca tatatcgttc ccggctttcg atttttttgt cgagaatcac
     1681 gcggtcgaac cgctcttttc cctcggcaag tttcctggcc aaatcgagat gggcccgggc
     1741 aataaagccg aagcggttaa tgtgcttctg gaccatgttt tccgcttcct caatccgttt
     1801 ggaaatttct acttcctgct cccgggtcag aagcggaacc tggcccatct gcctgagata
     1861 catccgaacc gggtcgtcca aaatatcgag cttgggtccg gttttctctt cctcttcttc
     1921 ctcttcttcg gcgtcctttt tcccgtcctt gtagcggtcc acctccgacg cttcaataat
     1981 gtcgatctcc atgcgacgga gtcgggtgag aatgaggtcg agctcatcgg cgtcggtgat
     2041 cccttcgggc aacgtctcgt ttagatcgtc aaaggtgaga tagccttgtt cttttgccag
     2101 tttgatcagt tcgcgaatgc ggctctgtat ttcaatcggg gaatgcagac gcgccattcc
     2161 ttcgctccgc ggctttggcg agagcgcgcc attgccagac tgtcctcgcg cgcgctcaat
     2221 gcgtttgggc ttggacgtgg cgagtcccgg ttgtctcttc agatttcgcc gaagagtcga
     2281 tgacttgctt gccagaccag ggcgaaggtg gcccttttta agccgtcgcg gcgacgactg
     2341 ccggctgcct gcctttcgtt tcgaatgcct ggccaagggt tgcggttaag gatttgactg
     2401 agacgcgcaa tcactgcctc ggatcaaccg tttagccgtc gatcctgctg gcagacgaaa
     2461 gcttcggaag ctcgcggagc tccttcacga ggtcaagaat ttctttctgc aaattcacca
     2521 gctcgccagt actcagctgc gggagcctga ttcggttctt cgccacatca agctggcggc
     2581 gcaacgcccc ctggcgaatg ccgagccacc atttttccac cattgttccg gcgttgggcg
     2641 gcactttttg gagcagccag gacgaaacca gcccctcatc tggcgctgaa agcgtagcca
     2701 tgaaggcgtt gagtgaggcc gcatcattcg gacgcaagtc gctttcgaga atgcgaacga
     2761 gaatttccgc gtccggcact tgggcgagaa cctgctgcca gttttgctga aggagaaaat
     2821 cccgggctgc ctcgtctcgc aaagccagga gacagagcat cgccatatcg tgccggggcg
     2881 gagtgccgga agtggcggga cgcgattcgt ccgcacgaat cccgctgctg cgggattgct
     2941 ttggaaagag cgtctggaaa tcctgaactg ggacgccgag ccgcgcgctc actttgcttg
     3001 ccacttcccc gcgcatgagc ggatcgtgca cacgggaaac ggtttccgcc aggcttcgcg
     3061 ctaactgcat ctttgcccct aacgaagcaa gatcgacttc ggcgacctca tgctcgatcc
     3121 agtaatcgaa aaaatcgcgc gcggccgcga tgcgcttctc aaattcttct tttccttccc
     3181 ggcgaaccag ggaatcggga tcgtcgcccg gggggatctc cgctacgcgc acgattaagt
     3241 cgttttcaag aagcgcgtcg agagagcgtt cggctgcttt cgcccctgcc gcatccgaat
     3301 cgaagcagag aaccacttca tccaccagtc gctttaacgt ccgtgcctga gtctccgtga
     3361 acgccgtccc ctggggcgca accacgtttg taattcccgc ctcaaacaga ctgatcaagt
     3421 cgagctggcc ttcgcacacg atcacacagt tcgcctcgat taaggcacgc ttggtcttgt
     3481 ccagaccaaa aagaacgctg cctttccgaa aaagcggcgt ttccggcgaa ttcaaatatt
     3541 tcgcggctcc ttctttctct ttgagaacac ggccactgaa cgcgatcacc tcgccaaggt
     3601 cgttgcagat cggaaacata atccggtcgc gaaagcgatc gtatgaggtt tgatgttcga
     3661 tttttgatgt ttgatctgct ttgtctttcg ttttgaccag accactttcg agcagctcac
     3721 ggggaccgaa tccttgccct cgcgcccaac tcccaaacgc gtcccattcg tccggggcat
     3781 aaccgagccg ccatcgtttc gcgatttcgg ctgtgattcc gcgttgcttc aaatattcgc
     3841 gagcaggttc ggcgaatttc ttctttagca ggttctcgtg aaaccattcc gcggcttctg
     3901 catgcacctt gagcagggta cgccgggcct catgttgccg attttcggcg tcccccgggc
     3961 cgcgctcttc gatcaccggg atcccaacgc gcgccgccag cttgcgcacg gcggaaggaa
     4021 aatcgatatg ctcgtaatcc atcacaaagc ggaaaacgct tccgcctgcc ccgcatccaa
     4081 agcagtggaa agtttgtcgc tgtgggctga catggaacga cggcgttttt tcctgatgaa
     4141 atgggcaaag cgccttgaaa tttgcacctg cgcgtttgag cggaaaatac gagccgatca
     4201 cttcgacgat gtcgtttgcg gccgcgacct gctcgatggt ttgtgatgga attgccacag
     4261 gcagatggca aacttactca gtgctgtgct gtttccaatg gagtattgga gtgcaggagt
     4321 aatggagtaa tgcgatgaat ccgccctttc actactccac tactccacta ctccattact
     4381 ccgctactcc ggttcgatga gcaaaatttt cgtgagcgcg ctgacgattt gggcagcgct
     4441 ctttctgctc gtgccggcaa gcgttcacgc gcgcgatgcc atcaataaag gcgacgtggt
     4501 ggtcgcgcca ttacgaggcg aagttgggcc ttctctcctg gcatttttgc gtcgggcggt
     4561 gaaaacagct gagagcaacg aagccgctgc gatcattttc gagatgaaca cacacggcgg
     4621 acggctcgat gcggcggccg acatcgtgag cgcgctcaat caaacaaaga tccccactta
     4681 cacattcata aacacgaatg ccggatccgc cggtgcgatt atcgccatcg ccacgcaaca
     4741 catttttatg gcgcccgtca gcgcgatcgg cgccgccgcc ccgatcctac ccacgggcga
     4801 agacctcccc acgaccgccc gagagaaaac gatttcgtat tggtctgccc tcatccgtgg
     4861 ttccgcgatc aaaaatggac acaatcccga cgtcgccgag gcgttcatga acaaggacaa
     4921 ggaggtaaaa atcggcgacc gcgttattca tcgcccggga gcagtgctta ctctcaatgc
     4981 ccaggaagca accgaacgca tcaatggcaa accgctgctc gcggaaggca tggccgattc
     5041 gattgccgat ctggtaaaaa aagccggact caaaggcaac gtcgtcgcga tcgaaccaac
     5101 cggcttcgag caaatcgccc tttggatcac tgcactcgcg cctttgctgc tgctgctcgg
     5161 aatccttggt gcttatctcg aattcaaagt ccctggagta ggctgggccg ggattatctc
     5221 cgcgatctgc tttgcacttt ttttcctcgg gcactacgtg gccggccttg ccggctggga
     5281 agtcgttgcg ctgtttgttc tcgggatcgt tctcgtcctg atcgaaatcc tttttttcgc
     5341 tcactcgaca atcgttttcg gcgtcctcgg cgttttcctc atgatcgggt ccctgctttg
     5401 gacaatgatc gatcgttacc cgcagcaacc gtttcttccg acaggcgaga tgctcgccat
     5461 tccattgctt aacatgttta tcgcgattgt gggatcgttg attgtgatcg ccctgctcgc
     5521 ccgttatctg ccgcgaacca gcctttaccg ccggtttgcg ctgatcgatt ccaacccacc
     5581 cggcccatcg ctggccgggg cgcctcgcca gtttgcgacc gcactttcgc tgacgcccgg
     5641 aatgcaaggc accgcggtca ccgtattgcg gccaagcggc aaagcgcgtt tcgccgatca
     5701 cgttgtcgat gttgtcaccg aaggcgagtt catcgccccg gaaacgccgg tcaccgtgct
     5761 tcagaccgat ggaatgcgag tggtcgtgaa aagcgcatct cagatctaac gagaacaaga
     5821 tgagctacgg cgagcgaagc gcgcggtgct acggattgaa aggtcgaagt catgcaaaga
     5881 tgggtaacac ggcggctcgc ggtcagctcg atcgctttgt tagatggtac cgtagcgctc
     5941 tcacggattg ctagaagatt gttgcatacg ggacctccga tccagcgtac cgccgacgat
     6001 cccccactga aatgcaccga ggaaaagtaa ggcaaccaag gagagaattt cagaagacca
     6061 tggccagcga agtaaataca aagcgagcga cgaaggaagg ctaagcatag ctgtccagta
     6121 ccaaccaagt ccgggatatt tcccccaatg caggtgctgg cgagcaaact cctcgaccac
     6181 aatcagcgtg ttgatagcaa tgaggatcaa tgcggctcga tgatctctaa aggttcgctt
     6241 taccatctaa cgggacggag ctcagccacc gctctgggag cgagactgcg caacagttga
     6301 ggatactcta atcaagatcg acatcgacgc gcaacgccaa agagcggttg gctggagcgc
     6361 tttgttagat gaccgacctg aaaaacacgt tgagttcatc gcgagcggct gcggacaaac
     6421 cgaacaagta accatacagc tacgactgca caaaacgccg aaagaaaata cgcttgcccg
     6481 ggccacatcc acgtatcacc gactccaccg agcttgtccg gcagaaacac gagcgcgcgg
     6541 tcatgcgcag cgatccacgc gcgagtgaac gctccaatcg agagcagaag gaacagcact
     6601 gcaaaggcga ggtacgaacg acgtgctttg ttctgctgcg ccatgtcatc taacgagaac
     6661 gaagtgagct accgccaccg agggcgagca atgctcgaag tgaggaggtt ttagtcatct
     6721 taaatgcgaa cgcgcagcgg gtggcggtta gctccagtga ctggttagac ctttgtggct
     6781 tcatgctcac gggcatttac tcgaatcgcc catcggacca tcagcgtggc ggcaatgaga
     6841 ccgaagacgc tgatgaaagg ccgcacaaca tattcggtat gagcgaagtt ggcataggcg
     6901 tgggctccga aaacgtccca cttaagcttt tcctcggata aagtgatgat cacgtgcagg
     6961 acgctgacga gcaaatatgc gataccgacg accagccaaa ggcgaaagaa tggtagatgc
     7021 gtgcgtctta ccgcgaacca ccagaggccg acagagtaaa tgtcgatgca gacgtatgcg
     7081 atggccatgc cagcgcgata ccagtcaatg taatcatcga gcatgaggtc taacgagaat
     7141 tatacgaaaa agtttcgtct aacaaggtcg agcaaattaa tcgtttttta gccgccggcg
     7201 acgacgcgga tgaattcgac gcggtcgcct tcggctaatg ggcgcgttgg ccattcgtgc
     7261 cgatggacgg cgaggccatt gtgctctaca aggatagatt gcggcggcaa ttgatggcgt
     7321 tccacgagct gcgcgattgt ttcagcgcca cgcgcatcca cggtttcgcc gttcagtgag
     7381 attgtcattc ggcaatacct tgaattcggc ggtcacagac cgccgctaca ggttaaatcg
     7441 taatgcgccg cagagagcct ggtcccaatc cttccatacc ggctcgaaac cgcgatgccg
     7501 aagaaccgct gcgatttcgg cgggagaacg ttcatcgctg atctcgaatt gaccagtcgc
     7561 gagttcgtct tctccatctt ggaactctgg ggcg
//
LOCUS       DASQUQ010000135         8487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_568325, whole genome shotgun sequence.
ACCESSION   DASQUQ010000135 DASQUQ010000000
VERSION     DASQUQ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568325"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /gene="queG"
                     /locus_tag="VE486_03825"
     CDS             complement(<1..1075)
                     /gene="queG"
                     /locus_tag="VE486_03825"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZA38248.1"
                     /translation="MPRISGVELKKELVAFARGLGFDSCRVAACAPPPHATEFRNWLR
                     EGAAGEMHYMERGEEKRCDPQKILPGARSIVVVALNYFQGPATPRSPASATLRRGKQT
                     AATGRIARYAWGDDYHNLIAARLDTIDNFLREFGGHQKCYVDTGPILERDHAAEAGIG
                     WHGKSTMLIDRRLGTWFFLGEMFTTLELPPDSPQPDRCGKCTRCIDACPTGAITAPRR
                     LDARRCISYLTIELKGAIPLEFRPLIGNRIFGCDDCLDACPWNHFAEISRESAFAARK
                     STPGMSLRDYLALGEAEFRVMFRDSPIKRIKRRGFLRNVCVALGNVGDPNDLPALERA
                     ASDPESLIAEHAAWAIERIGKRFA"
     gene            complement(1099..1629)
                     /locus_tag="VE486_03830"
     CDS             complement(1099..1629)
                     /locus_tag="VE486_03830"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZA38249.1"
                     /translation="MKLARSIALIGMMATGKSVVGKALEQKTGLPRFDTDEIISSKLK
                     VPIKEIFSTHGEEYFRNLETETLRSMSLEEPAIIVTGGGIVLRGENIDLLRQLATVVW
                     LDTDETTLRARIHNLRDRPLLQTANPGASLSELLAAREPLYRRAADLRVDVAQRSPED
                     IAELILENIKNFPVGE"
     gene            1731..2222
                     /gene="purE"
                     /locus_tag="VE486_03835"
     CDS             1731..2222
                     /gene="purE"
                     /locus_tag="VE486_03835"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZA38250.1"
                     /translation="MSKSKSLVAVLMGSKSDWETMHHCIDMLNELGIANEHHVLSAHR
                     TPDATADFARNAEERGLRVIITGAGSAAHLAGVVAGLTQLPVLGVPIPSSALHGLDSL
                     LSIAQMPGGIPVGTLAIGSAGAKNAALLAAAILALSDDKIRNNLQAFRKKQTDAVLAA
                     KLP"
     gene            2219..3289
                     /locus_tag="VE486_03840"
     CDS             2219..3289
                     /locus_tag="VE486_03840"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZA38251.1"
                     /translation="MKTETVAASHDRRSNRPPQTAAAIASVVALLQKGDPVALPTETV
                     YGLAANAINPLAVAKIFEAKERPRFDPLIVHLPDRDWLDRVAEICGEDRELVEKLINR
                     FWPGPLTIVLPKREIIPDIVTAGLDTVAVRVSAHPVFAQIIDAFGRPLAAPSANRFGR
                     VSPTTAQHVCDELDGRIPLIVDGGPTVHGIESTIVAVLDSKISILRRGPVTTEQLSEF
                     GKIEIVDPVGRVFAPGQLPTHYAPRTPLRLIEDAGAFSPEENQRVGLLAWNPVSGKGA
                     FQGASVTAASVHFAAIRHLSEKQDLREAAANVFRYLRELDQSGLDLIVAERVPLRGLG
                     AAIMDRLQRGAHGPLDTQDRPE"
     gene            complement(3183..4856)
                     /locus_tag="VE486_03845"
     CDS             complement(3183..4856)
                     /locus_tag="VE486_03845"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZA38252.1"
                     /translation="MKYVFITGGVVSSLGKGLAAASLGTLLEQRGLRVVFQKFDPYLN
                     VDPGTMNPFQHGEVYVLNDGAETDLDLGHYERFTNCILSRHNNLTSGQVYESVILKER
                     RGDYLGKTVQVIPHVTDVIKDRIRELSDESKYDVVITEIGGTTGDIEGLPFLEAIRQF
                     ILEVGAQNVVNMHVTLVPYIKAAHELKTKPTQQSVAKLREIGLQPQVLICRTEKPLDR
                     DVRQKLSMFCSVSEKAVIDARDVKNTIYEYPLMLHTEGLDQLACDLLQLKTPEPDLTD
                     WRKFVERVVSPKKRIKIAVVGKYIDLRDAYKSIYESLTHAAASQDCGIDLKLIDAESL
                     EQGVDGQLNDVAGVLIPGGFGVRGVEGKVNAARFAREHKLPYLGLCLGMQVATIEFAR
                     NVCGIKGANSTEFDKNTTEPVISLLEEQRGVRTKGGSMRLGTWPTQIVDGTLARKIYG
                     RGEVMERHRHRYEFNMRYRDRMSEKGFTVSGTSPDGTLAELIELRDHPYFLACQYHPE
                     FQSKPNKPHPLFRGFIQACLAYPADHAPRAAADPLSPPRVREEELVPQR"
     gene            complement(4853..5602)
                     /gene="kdsB"
                     /locus_tag="VE486_03850"
     CDS             complement(4853..5602)
                     /gene="kdsB"
                     /locus_tag="VE486_03850"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZA38253.1"
                     /translation="MNKAVGIIPARWSSTRFPGKPLHLIAGKPLLRRVWEQSTRAKNL
                     DAVIIATDDMRIAEAAFDWGAEVALTSPRHGSGTDRAAEVAKKAKNFACIINIQGDEP
                     LVDPRLIDRIVEKLRSDQNIDMVTAAHSFSNSTDASSPHQVKVVVDLCGNALYFSRAA
                     VPFPRDPSQAKYFRHQGIYGFRRETLLQFVKWKPTPLERAESLEQLRALENGVTVHVL
                     VTAKGSPGVDTLEDADALEQTLLRTKRKAVS"
     gene            complement(5599..6126)
                     /locus_tag="VE486_03855"
     CDS             complement(5599..6126)
                     /locus_tag="VE486_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZA38254.1"
                     /translation="MKSPAVFVDRDGTIMKDADYCSDPTQVQIFPGVAEALRRLKSNR
                     FKLIIITNQSGIGRGLFTIEQYRAVEAEVLRELGDGLIDATYFCPDVPGQHSSCRKPA
                     PGMIVQATREHRIDLSRSFFVGDKEIDAECGRNAGVRTIRVQTGFDRDTTGTIADWVA
                     PDFAGAAAIILDSSE"
     gene            complement(6199..7209)
                     /locus_tag="VE486_03860"
     CDS             complement(6199..7209)
                     /locus_tag="VE486_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZA38255.1"
                     /translation="MDLTRLQRTLDRASSKRIMVIGDLMLDEFVWGKVGRISPEAPVP
                     VVEVTGESFYPGGAANVARNLRELVDHVAIIGLLGKDRCGQQLRELLAERKIDISNTV
                     EDETFRTIVKTRIIARHQQVVRVDRERSAGLSAAQIKQVVAAVRKNIPETDAIIFEDY
                     GKGFLTTELVSQIAHEACTAGKIVAADPNPRHSVDWRGATVVKPNRAEAFLAAGIPWR
                     DPGDAPAEDVDLRRAGETLLEKWETSYVLITLGEHGMMLFQQKEAPHYIPTKARQVFD
                     VSGAGDTAIALFTLGLACEATPIEAAEIANHGSAVVVSKLGTATVTRDELIASFKNDA
                     KD"
     gene            7366..8166
                     /locus_tag="VE486_03865"
     CDS             7366..8166
                     /locus_tag="VE486_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38256.1"
                     /translation="MIPFIKCLKVSAIALSGLTLCAQGAQVPQHIDIKQLSKTVEDVV
                     VPLPNEIFGALNKLGAVNWKEHVRSDKGTNFTERPRIALLLGTVIADGFVAVQAEDAP
                     AVKEIGQRVLTLARGIGVGNSITPHAKAIIEAADKRNWEGVRQELDRTQNSVQQAMNE
                     VHDEKLSQLVSLGGWLRGTEVLTSVVTKHFSADGAELLHQPDLLSYFQTRLQGMPEFN
                     VPIIREIEDALVQVKPLIDVGNARIPPEAVKKINEITTRLGSGIVTRD"
     gene            8176..>8487
                     /locus_tag="VE486_03870"
     CDS             8176..>8487
                     /locus_tag="VE486_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38257.1"
                     /translation="MNAFVKVLVRGSFLLLSLIALQPAVFASVDDAQSFALQAAEPYV
                     KQGFQVREDYWGGDLGSGEKKAVRQQLFKGNEYWFWLGTEVERAKVSVHIYDSDGKLA
                     EE"
BASE COUNT         1869 a         2350 c         2310 g         1958 t
ORIGIN      
        1 tcgcgaagcg tttcccgatt cgctcgatgg cccacgcagc atgctccgcg atcaacgact
       61 cgggatccga cgccgctcgt tcgagcgctg gcaaatcgtt cggatcgcct acgttaccca
      121 acgccacgca aacgttccgc aggaaaccgc ggcgcttgat ccgcttgatc ggtgaatcgc
      181 gaaacatgac tcgaaattcc gcctcgccga gtgccagata atcccgcagc gacatacctg
      241 gtgtcgattt tcgcgccgca aacgcgcttt cccgcgaaat ttcggcgaaa tgattccacg
      301 gacatgcgtc caggcaatcg tcgcaaccga aaattcggtt tccgatcagt ggccgaaact
      361 cgagcggaat tgcgcctttc aactcgatcg ttagatacga aatgcatcga cgcgcatcta
      421 atcgacgcgg cgcggtgatc gcgcccgtgg ggcacgcgtc gatgcaacgc gtgcattttc
      481 cgcagcgatc aggttgcgga gaatccggtg gcaactcaag ggtcgtgaac atttcgccca
      541 gaaaaaacca cgtgcccagg cgtcgatcga tcagcatcgt gcttttcccg tgccaaccaa
      601 tgccagcttc ggctgcatga tcacgctcca aaatcggtcc cgtatcgacg tagcatttct
      661 ggtggccgcc gaattcgcga aggaaattgt cgatcgtgtc cagccttgcc gcgatcaggt
      721 tgtgataatc gtcgccccat gcgtagcggg cgattcttcc agtagcggca gtctgcttgc
      781 cgcgccgtag cgttgcggag gcgggcgagc gcggagtcgc aggaccttgg aaatagttca
      841 atgccacaac aacaatcgac cgcgcacccg gtaagatctt ctgcggatcg caacgtttct
      901 cttcaccgcg ctccatgtaa tgcatttctc ccgcggcgcc ttcccgcagc caatttcgaa
      961 actccgttgc atggggcgga ggagcgcaag cagcaacacg gcacgaatca aaaccgagtc
     1021 cgcgcgcaaa cgcgaccagc tcttttttta attccacgcc cgatattcgt ggcatagtgc
     1081 cggcctcaga tctcatgctc attctccaac agggaaattc ttgatgttct ccaggatcaa
     1141 ctcagcgatg tcttcgggac tcctttgggc aacgtccact cgtagatcgg ctgctcttct
     1201 gtagagcggt tcacgtgccg ccagtaattc cgatagactc gcgcccggat tcgctgtctg
     1261 caacagcggg cgatccctca agttatgaat acgcgcgcga agggtggtct cgtcggtgtc
     1321 gagccagaca actgtcgcca gttgccttaa gagatcgata ttttctcccc gcaacacgat
     1381 accgcctccg gtgacgataa tcgccggttc ttcgagggac attgagcgca atgtttcggt
     1441 ttccagatta cgaaagtatt cctcgccgtg agtcgaaaag atttccttga tcggcacttt
     1501 tagcttcgag gaaataattt cgtccgtgtc gaaccgaggt aatcctgttt tttgctccaa
     1561 ggcttttccg acaacagact tgcccgtagc catcatgcca atcagggcaa tcgatctggc
     1621 gagcttcacc ttgcaatatc aacaaggaac ggcgatttcc aaatcaccac ggatgcaaaa
     1681 ccgcgtctag aattctgtat gagaagctgt tagtttcgca tgcatttcct atgtctaaat
     1741 caaaatctct tgtcgccgtc ctgatgggaa gcaagtcaga ttgggaaacg atgcatcatt
     1801 gcatcgatat gcttaatgag cttggcatcg cgaacgaaca tcatgtcctt tcggcacatc
     1861 gcactccgga tgccacggct gacttcgcac gcaacgcaga ggagcgtggt ctgcgcgtca
     1921 ttatcactgg ggcgggaagc gctgcacatt tggcgggagt agtggccggt ttgactcagc
     1981 ttccagtgct gggcgtgcca attccatcat cagcgcttca cgggctcgat tcgcttcttt
     2041 ccattgcgca aatgcccggt ggtatcccag tggggacgct cgcaatcggt tccgccggcg
     2101 caaagaatgc cgcgctcctt gcggccgcga ttctcgcgct ctccgacgat aagatccgaa
     2161 acaatctcca ggcttttcgc aaaaagcaga ctgatgcggt gctcgctgcg aagttgccgt
     2221 gaaaacagaa actgtagcgg cgtcccatga ccgccgctcg aatcgaccac cgcagaccgc
     2281 cgctgccatc gcgtccgtag ttgcgttgtt gcaaaaaggc gacccggtcg ctcttcctac
     2341 tgaaacagtt tacggcctcg cggctaacgc gatcaatccg ctcgccgtgg cgaaaatctt
     2401 cgaggcaaag gaaaggccac gttttgatcc gctcattgtt catctgccgg atcgggattg
     2461 gctagaccgt gttgctgaaa tctgcggcga agatcgggag ttagttgaga aactgatcaa
     2521 tcgtttttgg ccaggaccgc ttaccatcgt tttgccaaag cgtgaaatta ttccagacat
     2581 tgtgaccgcc ggtctcgaca cggtcgcggt tcgagtcagc gcgcatcccg tgtttgctca
     2641 gatcattgat gcgtttggaa gaccgctggc tgcgccgagc gccaatcgat ttggtcgggt
     2701 gagtcctacg acagcgcagc acgtctgcga cgaactcgac ggacgcattc cgctaattgt
     2761 ggatggcggt ccaaccgttc acggaattga atcaacgatc gttgccgtac tggatagcaa
     2821 aatcagcatc ctgcgccgag ggccggtcac cactgagcaa ctgtctgaat ttggcaagat
     2881 cgaaattgta gatccggttg gaagagtttt cgcgcccggc caattgccca cgcattacgc
     2941 gccgagaacc ccgctccgcc tgatcgaaga cgcaggagca ttttcgccag aagagaatca
     3001 gcgagtcgga ttactcgcgt ggaatccagt gtcagggaaa ggcgccttcc aaggcgcctc
     3061 ggtcaccgcg gcgagcgttc actttgccgc gatccgccac ttgagcgaga agcaggacct
     3121 ccgcgaagca gccgcgaatg tatttcgtta tttacgcgag ctggatcaat ccggcctcga
     3181 tcttatcgtt gcggaacgag ttcctcttcg cggactcggg gcggcgataa tggatcggct
     3241 gcagcgcggg gcgcatggtc cgctggatac gcaagacagg cctgaatgaa tcctctgaaa
     3301 agcggatgcg gtttgttcgg tttgctttga aactccggat gatactggca ggccaggaag
     3361 tacgggtgat cacgcaattc aatcaattcc gctagcgtgc cgtcgggcga cgttccggaa
     3421 accgtaaagc ctttctcact catgcgatcg cgatatctca tgttaaactc gtaacggtgc
     3481 cggtgccgtt ccatcacttc gccgcgtccg taaatcttcc tggcgagtgt gccgtccaca
     3541 atttgcgtcg gccatgtgcc caggcgcatg cttccgcctt tggtgcggac accgcgttgt
     3601 tcttccagta gcgaaataac cggctccgtg gtgttcttat cgaattcagt gctgtttgcg
     3661 cctttaattc cacaaacgtt tcttgcgaat tcaatcgtcg ccacctgcat gccgagacac
     3721 agaccgagat acggaagctt gtgctcacgc gcgaaacgcg cggcgttaac ttttccttca
     3781 accccgcgaa cgccgaaccc gccgggtatc aaaacgccgg ccacatcgtt cagttggcca
     3841 tcgacaccct gctccaacga ttcggcatcg ataagtttga gatcgattcc gcagtcctgg
     3901 cttgccgccg cgtgggtgag tgattcgtaa atgctcttgt atgcgtcccg cagatcgatg
     3961 tatttgccga ccaccgcgat cttgatgcgc ttttttggac tgacgacacg ctcaacaaat
     4021 ttccgccagt ctgtcaaatc cggctcggga gttttgagct gcaaaagatc gcacgccagt
     4081 tggtccagtc cctcggtgtg cagcattagc ggatattcgt aaatcgtatt cttgacatcg
     4141 cgcgcgtcga tcacggcttt ttccgagacg ctgcaaaaca tcgacaactt ttgccgcaca
     4201 tcgcggtcaa gcggcttttc tgtgcggcaa atcaacacct gcggctgcaa accgatctca
     4261 cgcagctttg cgacgctctg ctgggtaggt ttcgttttta attcgtgtgc ggccttgatg
     4321 tagggaacaa gcgtgacatg catgttcacg acattttgcg cgccgacctc gagaatgaac
     4381 tgccggatcg cttccagaaa tggcagcccc tcaatgtccc cagttgtccc gccaatctct
     4441 gtgatgacga cgtcgtattt gctctcgtcg gagagctcgc gaattcgatc tttgatcacg
     4501 tcggtgacgt gcggaatcac ctgtacagtt ttgcccaagt aatcgccgcg ccgctctttt
     4561 aggataactg attcgtaaac ctgaccgctt gtcaggttat tgtgccggga aagaatgcag
     4621 ttggtgaacc gctcgtaatg gcccagatcg aggtcggttt ccgcgccgtc gtttaatacg
     4681 taaacctcgc catgctggaa cgggttcatc gtcccgggat caacgttgag atacggatcg
     4741 aatttttgga aaacaacacg caaaccacgc tgctcaagca acgtgccgag tgaagccgca
     4801 gctagccctt tgcccagcga actcacgacg ccacccgtga tgaaaacgta tttcatgaga
     4861 cggcctttcg ttttgtgcga aggagcgttt gctccagcgc atctgcgtcc tcgagcgtat
     4921 ctacgccggg cgatcccttg gcagtgacga gcacatgaac agttacccca ttttcgagcg
     4981 cgcgcaattg ctcgagcgat tccgcgcgct ccagcggggt cggtttccat ttcacgaact
     5041 ggagcagcgt ttcgcgtcga aatccgtaaa tgccttgatg gcggaagtac ttcgcctgcg
     5101 aaggatcacg ggggaatgga actgccgcgc gtgagaagta gagcgcgttg ccgcaaagat
     5161 cgacaacgac tttcacctga tgcggcgaag acgcgtcggt cgagttcgaa aacgaatgtg
     5221 ccgcggtgac catgtcaatg ttctgatcgg agcgcagttt ctcgacgatt cggtcgatta
     5281 aacgcgggtc cactaatggc tcatctccct ggatattgat tatgcaggcg aagttttttg
     5341 cctttttcgc gacttcggcc gcccgatctg tgccgctgcc atgtctcggc gatgtcaacg
     5401 ccacttctgc gccccaatca aaagcggcct cagcaatgcg catatcgtca gtcgcgatga
     5461 tgacggcatc caggttttta gctcgcgtgg attgctccca tactctacgc agaagtggtt
     5521 taccagcaat cagatgcagc ggttttcctg gaaaacgtgt ggaactccaa cgagcgggaa
     5581 tgatcccaac ggccttattc attcagaaga atcaagaatg atcgctgccg caccagcgaa
     5641 gtcgggtgca acccaatctg cgatggtgcc ggttgtgtcg cggtcaaatc ccgtttgaac
     5701 gcggattgtg cgcacgccgg cattccggcc gcattcggcg tcaatctcct tatcgccgac
     5761 aaagaatgaa cgggaaaggt cgattcgatg ttctcgcgtc gcctgcacga tcatgccggg
     5821 tgccggcttg cggcaactgg aatgctgacc gggaacatcc gggcaaaaat aggtcgcatc
     5881 gatcaaacca tcccccagtt cgcgcaacac ttctgcttcc accgcgcgat attgctcaat
     5941 tgtaaacaag ccgcggccga tgccgctttg gttggtaatg ataatgagtt taaagcggtt
     6001 cgatttcaaa cggcgcaatg cttccgcgac gccagggaaa atttgtacct gtgtgggatc
     6061 agaacagtaa tcggcatcct tcataatggt gccgtcgcga tcaacaaaaa cagcaggaga
     6121 cttcacgtca cgggaaatcc ggtttggcgt atggcttccg acactcggct gaagccaggt
     6181 gttaatgagc cagcggtttc aatcttttgc atcatttttg aaactggcaa tcagctcatc
     6241 tcgcgtgact gtcgctgtcc caagtttgct gaccacaaca gcgcttccat ggttggctat
     6301 ttccgccgct tcaatcggcg tcgcctcgca agcgagtccc agggtgaaca gagcaatggc
     6361 ggtatcgccc gctcccgaga catcgaaaac ctggcgcgcc ttggttggaa tataatgagg
     6421 cgcttctttc tgttgaaaga gcatcatgcc gtgttcaccg agcgtaatga gaacatagct
     6481 tgtttcccat ttttcgagca gcgtttcgcc ggcgcgtcta agatcgacat cttccgccgg
     6541 agcgtcaccc ggatcgcgcc agggaatgcc agcggcgagg aaagcttcgg cgcggttcgg
     6601 tttgacgaca gtggcgccac gccaatcgac agagtgtcgg ggattgggat ctgccgccac
     6661 gattttaccc gccgtgcagg cttcatgcgc aatttgcgaa accagctcag tcgtcaagaa
     6721 acctttgccg taatcttcga aaataatcgc gtcggtctcc ggaatattct ttcgaaccgc
     6781 tgcgacgact tgcttaattt gtgccgctga tagtccggca gatcgctctc gatcgacacg
     6841 cacgacttgc tgatggcgtg cgatgatgcg ggttttgacg atcgtgcgaa acgtctcatc
     6901 ctcgaccgta ttagagatgt cgatctttcg ttccgctaat aattcacgca gctgttggcc
     6961 acagcgatct tttcccagca aaccgattat cgcaacgtgg tcgacgagct cgcgcagatt
     7021 gcgcgcaacg ttggcagcgc ctcctggata aaatgattcg ccggtcacct ccacaaccgg
     7081 caccggagcc tcgggcgaaa tgcggccaac cttcccccaa acgaattcgt ccagcattaa
     7141 atcgccgatc accatgattc gcttggaaga tgcgcggtcg agagtccgct ggagccgggt
     7201 gagatccata aaaaaactac atggggatgc cgccgaggcg acgaattggt tcgattgaaa
     7261 tgagttaaat tgttttgggc aacgatttgt gtacagcagg ggttccgctt aaagtttgct
     7321 ccattaatcc tgaatacgaa gctaaaatca acagtcgaag ctccaatgat tccattcatt
     7381 aaatgtttaa aagtaagtgc aattgccctg agtggcctga cgctgtgcgc ccagggtgcg
     7441 caggtccctc agcacatcga tatcaagcaa ctgtcgaaaa ctgtggaaga cgtcgtcgtt
     7501 cccttgccga atgagatttt cggcgcgtta aacaagttgg gcgcggttaa ctggaaagag
     7561 catgtccgca gcgacaaggg aacgaatttt accgagcggc cgcgcatagc cctcttgctt
     7621 gggaccgtga tcgccgatgg ctttgtggcg gtgcaagcgg aggatgcgcc ggcggtaaag
     7681 gaaattggcc agcgtgtcct gacactcgcc aggggaattg gcgtcggcaa ttcaatcacg
     7741 ccgcatgcta aagccattat cgaggccgcg gacaagcgca attgggaagg tgtgcggcag
     7801 gaactcgatc gcacgcaaaa cagcgtgcaa caagcaatga acgaggtgca cgacgaaaag
     7861 ctgtcacagc tcgttagttt gggcggctgg ctccgaggca ccgaggtgtt gacctcagtc
     7921 gtgacgaagc atttttccgc tgacggcgcg gagctgctgc atcaacccga tcttctttca
     7981 tattttcaaa caagattgca gggcatgcca gagttcaacg ttccaatcat tcgcgaaatc
     8041 gaggacgcgc tcgtgcaagt caagcctctc atcgatgtgg gcaacgcgcg tattcctccg
     8101 gaagcagtaa agaaaatcaa tgaaatcacg acgcggcttg gcagcggtat tgtcaccaga
     8161 gattaggaat ctttaatgaa tgcgtttgtg aaggttctcg tgcgcggaag ttttctgctg
     8221 ttgtctctga tcgcattgca gcctgcggtt tttgcttctg tcgacgacgc gcagtcgttt
     8281 gctcttcagg ccgcggagcc ttatgtgaaa caaggatttc aagtgcgtga ggattactgg
     8341 ggaggcgacc tcggttccgg ggagaagaaa gccgtgcggc agcaattgtt taaagggaac
     8401 gaatactggt tttggttagg cacggaagtg gagcgggcga aggtttccgt tcacatctac
     8461 gattcggatg ggaagctggc cgaagaa
//
LOCUS       DASQUQ010000136         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_205993, whole genome shotgun sequence.
ACCESSION   DASQUQ010000136 DASQUQ010000000
VERSION     DASQUQ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205993"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="VE486_03875"
     CDS             complement(<1..1512)
                     /locus_tag="VE486_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HZA38258.1"
                     /translation="MPSLSLCYGTADCNPALEDRIRLGFPNEIFVKFCLPGRSPAKTG
                     IHPWLIFLLLFSAACQRTETDVERGNRLGILHKGNGQEVQDLDPQIVNSVSSLNVISA
                     LLEGLVSEDPHDLHPVPGVAESWEVSPDEKIYTFHLRRNAKWSNGNPVVAQNFLESYQ
                     RILTPALASPVAYMLYPVVNAEAFNKGKIDNFDEVGFRVLDDWTFQIKLASPTPYFLS
                     LLNHYSWFPVPISTIKKYGPVFERGSRWTKPGRFVGNGPFTLEQWRLNDRVRVKKSAT
                     YWDAQTVRLNEIVFHTIDSNDIEERAFRSGQLHVTDSMPINRIDRYRREQPEFLRIDP
                     YLGTYFYRVNVTRPPLNNRLLRRALAMAIDRQSIVERVTRGAQLPASCFTPPNTAGYT
                     CQSSIPYDVDLARRTLAEAGYPNGRGLPPIEILFNTSENHKLIAEAVQQMWRQNLNVN
                     ATLLNQEEKVYFDSRRQMNYQVIRSTWIGDYVDPNSFLDLWVTNGLNNQTGWSN"
     gene            complement(2031..2456)
                     /gene="rplK"
                     /locus_tag="VE486_03880"
     CDS             complement(2031..2456)
                     /gene="rplK"
                     /locus_tag="VE486_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZA38259.1"
                     /translation="MAKEVVAHIKLQIPAGQANPAPPVGTALGPRGVNIMAFCKEFNA
                     ATQKQAGDILPVVITVFKDKSFTFVTKSPPAAALLKKAANIAAGSKEPNKTKVGKVTR
                     KQVMDIVNIKRKDLNARNDEAAFRIIAGTARQMGLEIAD"
     gene            complement(2562..3131)
                     /gene="nusG"
                     /locus_tag="VE486_03885"
     CDS             complement(2562..3131)
                     /gene="nusG"
                     /locus_tag="VE486_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZA38260.1"
                     /translation="MAQTLAGKDQWYVVHVLSGQENKVRENILKRVKTEEMGDLVYEV
                     LVPTERVSEIKKGKKSETTRKFFPGYLIVNMHLLDENNQLVGRTWYFIRDTPGVIGFA
                     GTKDKPIPMRQSEVDSMLAQMKEREEKVKPKVSFEVGESVKVADGPFQNQTGIVEEID
                     PERGKLRVSVTIFGRATPVELEYWQVERG"
BASE COUNT          659 a          851 c          893 g          770 t
ORIGIN      
        1 gtttgaccag ccagtttgat tgttcagccc gttggttacc caaagatcga gaaacgagtt
       61 gggatcgacg taatccccga tccatgtcga acgaatcacc tgataattca tctggcgccg
      121 cgaatcgaaa tagacctttt cctcctgatt caggagcgtg gcgttcacat tcaagttttg
      181 acgccacatc tgttggactg cctccgcgat gagtttgtgg ttttcggacg tgttaaacag
      241 aatctcgatc ggtggcaggc cgcggccatt tggataaccg gcttccgcga gcgttctgcg
      301 tgcgagatcg acatcatacg gaatcgagga ctgacaggtg tagccagccg tgttcggcgg
      361 tgtaaaacag gaagcaggca actgtgcgcc gcgtgtgact cgttcgacga tgctctggcg
      421 atcgatggcc atggcaagcg cccgccggag aaggcgatta ttcagcggtg gccgcgtcac
      481 gtttacgcgg tagaagtaag tgccgagata tggatcgatc ctgaggaatt caggttgttc
      541 gcggcggtag cgatcgattc gattgatcgg catggaatcg gtgacgtgca actggcctga
      601 gcgaaacgcg cgttcctcga tgtcgttgct gtcgatggta tgaaagacaa tttcatttaa
      661 ccgaacagtc tgcgcgtccc agtaagtcgc gctctttttc acccgcacgc gatcgttcag
      721 tcgccattgt tccagtgtaa acggtccgtt tccgacgaac cgacccggct tggtccagcg
      781 actgccgcgc tcaaacactg gtccatattt tttgatcgtg gaaatcggca caggaaacca
      841 ggagtaatga ttcagcagcg aaagaaaata gggcgttgga ctggctagct tgatttgaaa
      901 tgtccaatca tcgagaacgc gaaagccgac ctcgtcgaaa ttgtcgatct ttcccttatt
      961 aaaagcctcg gcgtttacga ccggataaag catgtaagcg accggcgaag ccagagcagg
     1021 cgtgagaatc cgctgatagg attcgagaaa gttttgcgcg accacggggt tgccgttaga
     1081 ccatttggcg ttacgccgga gatgaaacgt ataaatcttt tcgtctggcg aaacttccca
     1141 gctctcggcc acgccgggga ccggatgaag atcgtgtgga tcctcggaaa cgaggccttc
     1201 caaaagcgcc gagatgacgt tgagggaact aacgctgttt acgatttgtg ggtcgagatc
     1261 ctgcacttcc tgcccgttac ctttatgcag aatcccgagc cgatttcctc tttcgacatc
     1321 cgtctcagtg cgctgacaag cggcgctgaa aagcaggaga aaaatcagcc atggatgaat
     1381 accggtcttc gccgggcttc ggccgggcag gcagaatttc acgaatattt cgttcgggaa
     1441 tcccaaccgg attcggtcct caagcgcagg gttgcaatct gccgtgccgt agcagagcga
     1501 gagagagggc acgagctacc ctgggtggcc gttccgcaaa catccttaac gccgacgggg
     1561 tcgcgtcgat tcccattttc gttggcaacg tgagattcct gccgccttgt cattttcatc
     1621 gaagatagcg tcgaaggcct tcggggcaac cgcctagcgg ctgcaccgcc ttttgggatt
     1681 ggccggacaa gcgcgctcgg attttctcac ggaacgaatc acgttcacgc tggcgaatcg
     1741 cgcatttacc tcgttttctc gaccaaagaa cggcgatcgc tcctacgcta cgaagccacg
     1801 cgcagcgcgg tacaagcgta tcttggtggc gccctaaaca actcccttgt ccgctcatct
     1861 tcgtgggcgg cgtggaagcc acgttcatct gcgtgcacga ttcagacgca caaaagccgg
     1921 atgcggaaaa ttcgcatgtc cggtttgagg ggggagggaa cgcggcggcc attagctacc
     1981 gcctttcact ccgtacgtcc ctccctgctc taccgggatt tgcggttgga ttaatcggcg
     2041 atttcgaggc ccatttggcg ggcggtgccg gcgatgatgc ggaaggcggc ctcgtcgttg
     2101 cgcgcgttga ggtctttgcg cttgatgttc acgatgtcca tcacttgttt gcgcgtgact
     2161 ttgccgactt tggttttgtt tggctctttg gaaccggcag cgatgtttgc tgcctttttc
     2221 aaaagggctg ccgcgggggg acttttcgtg acgaaagtga acgacttatc tttgaaaaca
     2281 gtgatgacta ccggaagaat gtcgccggcc tgtttctgcg tcgcggcatt aaactctttg
     2341 cagaacgcca tgatgttcac gccacgcggg ccgagcgcgg taccaacggg cggcgccggg
     2401 ttcgcctgac cagcgggaat ctgcaatttg atgtgagcta caacttcttt agccataaac
     2461 taattcgtca ttctgagcgg agcgaagcga gtcgaagaat cccgtggcat tgccttgggc
     2521 ttcgcatcgg gatgtctcga cttcgctcga catgacaact cttatccccg ttccacctgc
     2581 cagtattcca attccaccgg cgtggcgcgg ccgaagatcg tgaccgagac gcgcagtttg
     2641 ccgcgttcgg gatcgatttc ttcgacgatg ccagtttggt tttggaacgg gccatccgca
     2701 acctttaccg attcgcctac ttcgaagctg acttttggtt tcaccttttc ttcgcgctct
     2761 ttcatttgcg cgagcatgct gtccacttcc gattgacgca tcgggatcgg tttgtccttg
     2821 gtcccggcaa aaccgatcac gccgggtgtg tcccgaatga aataccaggt gcgcccgaca
     2881 agctggttgt tctcgtcgag caaatgcatg ttcacgatca aatagcccgg gaaaaacttg
     2941 cgggtcgttt cgctcttttt gcccttcttg atttctgaga cgcgctcggt cgggacgagg
     3001 acctcgtaga ccaaatcgcc catttcctca gtcttgacgc gctttaagat gttctcgcgc
     3061 accttgtttt cctgtccgga aagcacgtgc acgacgtacc attgatcttt gcctgcgagc
     3121 gtttgtgcca taaatcagtg cagccgggtg aaaaagtgga cgaagttcac cag
//
LOCUS       DASQUQ010000137         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_496961, whole genome shotgun sequence.
ACCESSION   DASQUQ010000137 DASQUQ010000000
VERSION     DASQUQ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496961"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VE486_03890"
     CDS             complement(<1..428)
                     /locus_tag="VE486_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38261.1"
                     /translation="MSFIRTGFREIALKVKRQRTRIALRHEKRLLQKSEINLGREGTA
                     QAASFPELRNEIVALKKLEQEQKEVALRIAQIEEGIKKIEEERQQNTREQHEAIAKLE
                     AEKKPLVHQHNQAKNAVDVCDRELTAVGRRIQENEAADRDL"
     gene            complement(556..2502)
                     /gene="acs"
                     /locus_tag="VE486_03895"
     CDS             complement(556..2502)
                     /gene="acs"
                     /locus_tag="VE486_03895"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZA38262.1"
                     /translation="MHNDIESHLIEKRVFKPSKGFSKTARIKSPAQYRRMYRESIRQP
                     AKFWGREASELVWRAPWKKVLEWKAPFAKWFVGGKLNISENCLDRHLNGPRRNKAAIL
                     WEGEPGDKRTLTYQQLYREVCRFANVLKRNKIKTGDRVIIYLPNIPEAAIAMLACARI
                     GAVHSVIFGGFSADSIRDRIIDSGAIALVTADGAYRRGAIVPLKKNVDEALRGGTSIK
                     RVIVFRRTGSEIHMEEVRDIWWHQELEYVDANCPPVPLDSEHPLYLLYTSGSTGKPKG
                     ILHTTGGYLVGVYSTMKYVFDIRDEDIFWCTADVGWVTGHSYIVYGPLANGATVVMYE
                     GAPNWPEPDRFWKIIEDYRINILYTAPTAIRAFIRWGDQWVAKHDLSSLRLLGTVGEP
                     INPEAWMWYHENIGGGRCPIVDTWWQTETGSILITPLPGAIPTKPGSATLPFFGVEPA
                     IVDEKGDEVGPNVGGKLIIQRPWPAMLRSIYGDKARYRQQYWSEFKGNYLTGDGARRD
                     EDGYFWIVGRIDDVLNVAGHRLGTSEIESALVSHARVAEAAVVGRPDELKGQGVVAFV
                     TVKTGVDASRGLKEELRDHVAAHIGAIAKPDEVRFAEALPKTRSGKIMRRLLKEIASG
                     AKVTGDTTTLEDFSVLARLSESEE"
     gene            complement(2675..3319)
                     /locus_tag="VE486_03900"
     CDS             complement(2675..3319)
                     /locus_tag="VE486_03900"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HZA38263.1"
                     /translation="MKKLSFILALLSLSLVTAGAAEQDTVNHCAAIIRDFRQMPERGI
                     PRDVLRHAKGLAIMTVVKAGFIFSGKGGQGVVVARTDHGWSGPSFIATGGAGWGLQIG
                     AQVTDFVIVLNNDKAVQAFARGGNVTIGADLSAAAGPVGRTAAGAVTPTAAVYTYSKS
                     KGLFAGVSLEGAVIGTQRQSNFRYYGGPVRADEILSGVTSPPPGAAPLRKALGR"
     gene            3629..>4051
                     /gene="tadA"
                     /locus_tag="VE486_03905"
     CDS             3629..>4051
                     /gene="tadA"
                     /locus_tag="VE486_03905"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HZA38264.1"
                     /translation="MIDLPSDEAFMREALRQAQKAYEADEVPVGAVVARAGKIIARAY
                     NQVELLKDATAHAEMLALTQAEAAVGDWRLTDCDLYVTKEPCAMCAGALVHVRIHRVV
                     FGCADPRAGAAGSVINLLQLPSLNHQCEITAGVLRNECA"
BASE COUNT          822 a         1169 c         1099 g          961 t
ORIGIN      
        1 agatcgcgat cggccgcttc gttttcctga atgcggcgtc ccaccgccgt cagctcgcgg
       61 tcgcagacat caaccgcgtt cttcgcctga ttgtgttggt ggacaagcgg ctttttctcc
      121 gcttccaact ttgcaatggc ctcgtgttgt tcgcgcgtat tttgctggcg ttcttcctcg
      181 atctttttga ttccttcttc gatctgggcg atccggagcg cgacttcctt ttgttcctgc
      241 tccaatttct tgagtgcgac gatctcattg cgcagctcgg gaaaactggc cgcctgcgca
      301 gtgccttcgc gtccgagatt gatttcactt ttttggagca ggcgtttctc gtgacgcaac
      361 gcaatgcgcg tccgctgccg cttaactttc aaggcgatct cgcgaaagcc tgtgcggatg
      421 aagctcacag gctaacgcta tacgaatgca ggcattgaaa aagaaatttc cgcccgcacg
      481 ggctaacggc ggtgcgaaaa ttcattggac ccattcgtcc catgagtcgt atgggactga
      541 tgggacatat ggattttatt cttccgactc gctcaatctt gccagcacgc tgaagtcctc
      601 cagtgtagtg gtatcgccgg tgacttttgc gccgcttgcg atttctttga gaagccgtcg
      661 catgatttta ccgctgcgcg ttttcggcag cgcttcggca aagcgcactt cgtccggctt
      721 cgcgattgcg ccgatatgcg cggcgacgtg atcgcgtaat tcctccttta gtccacgact
      781 ggcatcaact cctgtcttca ccgtgacaaa cgccacaacg ccctgacctt ttaattcatc
      841 cggccttcca accacggctg cctcggccac tcgtgcgtgg ctgacgagcg cgctttctat
      901 ttcggacgta ccaagtctgt ggccagcaac attcagaaca tcatcaatcc ggccaacgat
      961 ccagaaatat ccgtcttcat cacggcgggc gccgtcgcca gtgagataat tgcctttaaa
     1021 ctcactccag tattgctgtc gatagcgcgc cttgtcgcca tagatcgagc gcaacattgc
     1081 cggccacgga cgctggataa tcagttttcc gcccacgttc ggccccactt catccccttt
     1141 ttcatcgaca atggctggtt cgaccccgaa gaagggaagc gttgcgctgc caggcttcgt
     1201 tggaatggcg ccgggaagag gcgtgatcaa aatcgaaccg gtctcagttt gccaccatgt
     1261 atcgacaatc ggacaacgcc cgccgccgat attctcatga taccacatcc atgcttccgg
     1321 gttaatcggt tcaccgaccg tcccgagcag gcggagagac gagagatcgt gttttgccac
     1381 ccattgatcg ccccagcgaa taaatgcgcg gatcgctgtt ggagcggtgt aaagaatgtt
     1441 gatgcgatag tcttcgatga ttttccagaa tcgatcgggc tccggccaat tgggcgcccc
     1501 ctcatacatg acgacagtcg cgccattggc aagtgggcca taaacaatgt aactgtgccc
     1561 tgttacccag ccgacatcgg cggtgcacca aaaaatatct tcatcgcgga tgtcgaagac
     1621 gtacttcata gtcgagtaaa cgccgaccag ataaccgccg gtggtgtgca ggattccctt
     1681 gggcttccct gtggaaccgc tcgtgtagag aaggtaaagc gggtgctcgc tgtcgagagg
     1741 aaccggcggg cagtttgcat cgacatactc cagttcctga tgccaccaga tatcccggac
     1801 ctcctccatg tggatttcgc ttccggtgcg gcgaaaaacg atcacccgtt tgatcgatgt
     1861 gccgccgcgc aatgcctcat ccacattttt ctttagaggc acaattgcgc cccggcgata
     1921 tgcgccatcg gcagtgacca gagcgattgc gccgctgtcg ataatgcgat cacgaatgct
     1981 gtccgcgcta aatccaccga agatcaccga atgcaccgcc ccgatacgcg cacaggccag
     2041 catggcgatc gccgcctccg gaatattcgg cagataaatg atcacgcgat cgcccgtctt
     2101 gattttgttt cgtttcagga cattcgcgaa tcggcagact tcgcgataca attgctgata
     2161 ggtgagagtg cgcttgtcgc ccggttcgcc ctcccagagt atcgctgcct tgtttcggcg
     2221 cggaccgttt agatgccggt cgagacagtt ttcagaaata ttgagtttgc cgccgacaaa
     2281 ccacttggcg aatggcgctt tccattcgag aacttttttc cacggcgcgc gccagaccag
     2341 ttcgcttgcc tcgcgccccc agaatttggc cggctgccgg atcgattctc gatacattcg
     2401 ccgatattgc gccgggcttt tgattcgggc cgttttcgaa aaacctttcg atggtttgaa
     2461 aacgcgcttc tcgatcaggt gcgattcgat gtcgttgtgc atgggcagtc cctaatatgc
     2521 cgaagaaatt ctcagggcaa gcggtctgtt cttttcttgt agccacgtcg ctgtgcgacg
     2581 tccggttgga aagcaccccc atttgtcagg tggaggaacc ggccacaggc ccggttgcta
     2641 acagcaatct cttttggtct tgaatcgtcg cagttcagcg gcctagcgcc ttgcgcagcg
     2701 gcgcggcgcc tggtggcgga gatgtgactc cactcagaat ctcatccgcc ctgaccggac
     2761 cgccgtaata gcggaaattc gattgccgct gcgttccgat taccgcgccc tccagggaga
     2821 cgccggcaaa gagacccttg ctcttgctat aagtgtaaac agcagccgtc ggggtgacag
     2881 cgccggccgc ggtgcggccc accggccctg ccgcagcgct gagatccgcg ccgatggtca
     2941 cgtttcctcc acgggcaaat gcctggacgg ccttgtcatt gttgagaacg atcacaaaat
     3001 cggttacttg cgcgccgatc tgaagacccc agcccgcgcc gccggtggcg ataaatgatg
     3061 gaccggacca gccatgatct gtgcgagcga ccacaacgcc ctggcctcct ttgccgctaa
     3121 aaataaatcc ggctttaacg acggtcatga tcgccaggcc cttggcatgt cgcagcacgt
     3181 ctcgtggaat tccgcgttcc ggcatctgac gaaaatcccg aatgatcgca gcacagtggt
     3241 taacggtgtc ctgttcggcg gcgccggcgg tcacgagcga gagagacaat aaggcaagga
     3301 taaacgataa ttttttcatt ggattacgat taacttcgct agcgtaactc agcgcggatg
     3361 tatttggcaa agcagatatc cgctgtagcc gcctcgctca tccgcctttc tcttgcgctc
     3421 gttctttttc atctgtcatt ccgaccgaag tggaggaatc tcttgccgtt tgatcgtcgg
     3481 ttccttaccg gaaaacaatg agagatgtct cgactctgct cgacatgaca aagatcgacc
     3541 cgggtgctgc ttttatgacc gttggacggt tcagccgcct tggcgatact ggggcgtgcc
     3601 aggcataacc gtggctacaa cttgaatgat gattgatctg ccaagcgatg aagctttcat
     3661 gcgcgaagcg ttgcgacagg cgcaaaaggc ttacgaagcc gatgaagtgc cggtcggtgc
     3721 agtcgttgcg cgggcgggaa aaattatcgc gcgcgcctac aatcaggttg aattgctgaa
     3781 agacgcaacc gcgcacgccg aaatgctcgc gttgactcag gcggaggctg ccgtgggaga
     3841 ttggcgtctg accgattgcg acctttacgt gacgaaggaa ccatgcgcga tgtgcgcggg
     3901 tgcactcgtt catgtgcgaa ttcaccgtgt tgtttttggt tgcgcagacc cgcgcgccgg
     3961 agcagccgga agcgttatca acctcttgca actgccgagt ttaaatcacc agtgcgagat
     4021 caccgctggt gttttgcgaa atgaatgcgc g
//
LOCUS       DASQUQ010000138         4744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_756985, whole genome shotgun sequence.
ACCESSION   DASQUQ010000138 DASQUQ010000000
VERSION     DASQUQ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756985"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VE486_03910"
     CDS             complement(<1..554)
                     /locus_tag="VE486_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZA38265.1"
                     /translation="MNEPDQENQHAPLSYHGAWRIVWLVFLLIIIWLLLRSLQPVILL
                     FALVFLLAMVLNPIVVWLHKWHIPRFISVILLVFALIAVVGTIVLFAIPPLARQSQEL
                     MRSAPGVWQGIRIRIESLTENYPTVRDALPRSEEIAGHVGAAAGKVGSILLRSTLGLV
                     GGVASVVFALLLLIFVLANPRPLVT"
     gene            584..718
                     /locus_tag="VE486_03915"
     CDS             584..718
                     /locus_tag="VE486_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38266.1"
                     /translation="MTRKHDRPSGAGTEKDPCFVSVVYPVRTDDYRLDVSQGFDLSTL
                     "
     gene            880..2157
                     /locus_tag="VE486_03920"
     CDS             880..2157
                     /locus_tag="VE486_03920"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZA38267.1"
                     /translation="MTRSYLPPIFIVGQYKCGTSWLARILSAHPEVIGVHEIDIVDAS
                     CKVKNGAVTLAPMQQRLDRFFDKADWCNDYTPSGWEYADVVARFERGEEIPTRSWDRS
                     KPLKFMHLRAETARWLYNEIKAAAAPEQAMNAFLTAVCANAERESHIVLKAADQLWNF
                     DILQSWQPNAKKIAITRDGRDASISARHFQDLMREVKPSRGVPRIHAYLDLLRKWADR
                     ADRAITAAGRGRLYVLRYEDLTNDYTATLTRLLDWLELPHSQPLIDTIQKQTCFETLT
                     GRARGVEAKGFIRKGAVGEWRHVFSVPEQEQAWRVAGEQLSALGYTREGTLRPLPDLS
                     NANEYPYRLERNLQLQQKVAALRARLRLAEAQVREFKSLSKKKVSPGARWTRPLQATL
                     RAARKATKHLARFFVSTITFATPSCSDALSIGW"
     gene            2309..4471
                     /locus_tag="VE486_03925"
     CDS             2309..4471
                     /locus_tag="VE486_03925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZA38268.1"
                     /translation="MKALVTLALFVAATAIAQTPSPTSAPGKHSFTFEDMMKLKRLDG
                     PVPSPDGKWVIFSATDVDLAANTKTSHLWIVPASGGESRRLNETSNHEERPRFSPDGK
                     RLIWTSKATDPTQIWMCDFDPEAGKLVGKPDQVTSISTGADGGIWSPDGKSIVFVSAV
                     YPDCKDDACNKQRDEELKKSKVKAKIFTKLFYRHWSAFTEFKRSHLFVQSVGRDSVEP
                     TKTDGAKKFDGFAERRPTGNSEPRDLTPGDHDVPPFNLGGQDMYAISPDGQEVAYTSN
                     IDEVEATSTNNEIFIVPMAGPAVAGKKISTSPGADTTPLYSPDGKYLAWRSQARAGFE
                     ADKWRLLVQDRQSGKTRDLNQKFHRSVGSFTWFSDVSNGGLFFAAEDNGESPIYALFL
                     DREQPTRGPQLHADDLIWQENTSTLFFTRMSIAAPNEIWRFALSGETLGGTVKPEPVT
                     HMNDALLSQIDMQPLESFTFKGANGEEVQGFMVKPPGFDPNKKYPLKFLIHGGPQGAW
                     GNSWSYRWNPELFAANGYVVVMINFHGSTGYGQKFTDSISGDWGGKSYVDLMKGLDYV
                     EKTYPFIDKNREAALGASYGGYMANWILGHTNRFRCIVSHDGVFNAESAYGTTEELWF
                     PEWEFKGPPWKQRELYRKFSPHLFADKFKTPTLVIHGQNDYRLDVSQGFDLFTTLQRL
                     KVPSEMLYFPDEGHWVLKPQNSRLWYKTVNDWVDRWCK"
BASE COUNT         1112 a         1279 c         1369 g          984 t
ORIGIN      
        1 gtgacgagcg gccggggatt ggccaataca aaaatgagca ggagcagggc gaaaacgacg
       61 ctggctacac cgccgaccaa accaagtgtc gatctcagta aaatgctccc gacttttccg
      121 gcggccgcgc cgacgtgtcc ggcaatttcc tcactgcgcg gcaacgcgtc ccgaacggtc
      181 ggatagtttt cggttagcga ttcgatgcgg atgcgaatgc cctgccaaac tcccggcgca
      241 ctgcgcatca gttcctggct ttgtctggcc agaggcggaa tggcaaagag aacgatggtc
      301 cccacgactg cgattaacgc aaacaccagg aggatcacgc ttatgaagcg cggaatatgc
      361 catttatgca gccagacaac gatcggatta aggaccatgg ctaggagaaa aacgagcgcg
      421 aagagcaaaa tcacgggctg aagcgaacgc aaaagcaacc agatgatgat tagcaaaaaa
      481 acgagccaga cgattcgcca tgcgccgtga taactcagcg gagcgtgctg attttcctga
      541 tcgggctcgt tcataacgtc gatcttggcg cccgcgattc ggaatgacaa gaaagcatga
      601 tcgcccttcc ggcgctggca cagaaaaaga cccgtgtttc gtgtcggtgg tttatccggt
      661 tcggacggac gattatcggc tcgacgtgtc gcaaggtttt gatctatcta cgctgtaaca
      721 ctctaagtgc cgtcgaagat gttgtattcc cggatgaaga tcaacaaatg ctcaaacggc
      781 aaaacgcgca gatggaatta caaaacagtg aacgactgag tcgactattg attcgaccgc
      841 gacgcgccgg ccgcgacaaa ccaattctcg cgatctgata tgacacgctc ctatttgccg
      901 ccaatcttta tcgttggcca gtacaagtgt ggcacgtcgt ggctggcacg gatcctcagc
      961 gcgcacccgg aggtcatcgg cgtccacgag atcgatatcg tggatgcatc gtgcaaggtg
     1021 aaaaatggcg cggtcacact tgctccaatg caacagcggt tggaccgatt tttcgacaag
     1081 gcggactggt gcaatgatta cactccgtcc ggctgggaat atgctgacgt ggttgcgcgc
     1141 ttcgagcgtg gagaggaaat tccaacgcga agctgggatc gcagtaagcc gctcaaattt
     1201 atgcatttaa gggcggagac agcgcgatgg ctttataacg aaatcaaagc ggctgctgct
     1261 ccggagcagg cgatgaacgc gtttctcacc gccgtttgcg cgaacgccga acgagaatca
     1321 cacatcgttt taaaagcggc cgaccagctt tggaactttg acatcctgca gtcctggcaa
     1381 ccaaacgcga agaaaatcgc gatcacgcgg gacggtcgcg atgcatcgat ttctgcgcgc
     1441 cattttcaag acctgatgcg ggaagtgaaa ccatcgcgtg gtgtgcccag gattcacgct
     1501 tatttggatt tactacgaaa gtgggcggat cgggccgacc gcgcgattac tgccgcgggg
     1561 cgcgggcgac tgtatgtgct ccgttatgaa gacctgacaa acgattacac ggccaccctc
     1621 acgcggctct tggactggct cgagctgccg cattcgcagc cgctgattga taccattcag
     1681 aagcagacgt gctttgaaac gcttaccggc cgcgcgcggg gcgttgaggc gaaaggtttc
     1741 attcgaaagg gcgcagtggg cgaatggcgc cacgtgttca gtgtgcctga gcaggagcag
     1801 gcgtggcgcg tcgccggcga acaactgagc gcgcttggtt acacgcgcga gggcacactg
     1861 cgaccgctgc ccgatttaag taacgccaac gaatatccat atcgtttaga acgtaacctg
     1921 cagctccagc aaaaagtcgc ggcgctccga gcccgtctcc gcttggccga ggcgcaagtg
     1981 cgcgagttca aatcgctgag caaaaagaaa gtttcgccgg gcgctcgctg gacgcggccg
     2041 cttcaggcga ctctgcgcgc tgcgcgaaaa gcaaccaagc atttggcccg attttttgtc
     2101 tctacgatca ccttcgctac tcccagttgc agcgacgcgc tctccatcgg ctggtgatcc
     2161 gccgactgtt tggcctgttt ccacgcggcg ctttttcgcg agttgcacca ggcatctcgg
     2221 cacgctgccg agatcgacac gctggcagcg tgtgctcccc agatcagagc agttgcgcac
     2281 tggaaattgc aatcggactt tgcgcgtcat gaaagcgctc gtgactctcg ctcttttcgt
     2341 agccgccaca gcaatcgccc aaacgccctc gccgacctcg gcgcccggga aacattcgtt
     2401 cacattcgaa gacatgatga aattgaagcg tttggacgga ccggtcccgt cgcctgatgg
     2461 caaatgggtg attttttcgg caaccgatgt cgatctggca gcgaacacga agacatcgca
     2521 tttatggatc gtgccggcga gtggcggtga atcgcggcgg ttgaacgaga cgtcgaatca
     2581 cgaggagcga ccgcgatttt cgcccgatgg aaaacggctc atctggacgt caaaagcgac
     2641 cgatccgacg caaatctgga tgtgtgattt cgaccccgaa gcggggaagc tggttgggaa
     2701 accagatcaa gtcacatcga tctcgaccgg cgccgacggc ggaatctggt cgcccgacgg
     2761 gaagagcatt gtgttcgtgt cggcggttta tccggactgc aaggatgatg cgtgtaacaa
     2821 acagcgcgac gaagagctga agaagagcaa agtgaaagcg aaaattttca cgaagctgtt
     2881 ctatcggcat tggagcgcgt ttaccgaatt caagcggagt catttgtttg tccaatcggt
     2941 agggcgagac tctgtcgagc cgacgaaaac cgatggggcg aagaaattcg acggcttcgc
     3001 agagcgtcgc cctaccggca atagcgagcc acgcgatctt actccgggcg atcacgatgt
     3061 gccgccgttc aatctcggtg ggcaggacat gtacgcgatt tcgcccgacg gccaggaggt
     3121 cgcttacacc agcaacatcg acgaagtcga agcgaccagc acgaacaacg aaattttcat
     3181 cgtgccgatg gctggtcccg cagtggcggg aaagaaaatt tccaccagtc ccggcgccga
     3241 caccacgccg ctctattcgc cggatggaaa atatctcgcc tggcgctcac aagcgcgcgc
     3301 cggtttcgaa gccgataaat ggcggctgct cgtgcaagat cgacaatcgg gaaaaacgcg
     3361 tgatctcaat caaaagttcc atcgctccgt tgggagcttc acatggttct ccgatgtcag
     3421 taacggcggg cttttttttg cggcggaaga taatggtgaa tctccgatct atgcgctctt
     3481 tctagatcgc gaacagccaa ctcgagggcc gcagctgcat gctgatgatc tcatctggca
     3541 ggaaaacacc agcacactct tctttacccg aatgtctata gctgcgccta atgagatttg
     3601 gcgttttgcc ctttcgggag aaacgctcgg aggaaccgtc aagcccgaac ccgtcaccca
     3661 catgaacgac gcactgttgt cgcagatcga catgcagcca ttggagtcgt tcacattcaa
     3721 aggcgcgaat ggcgaagagg tgcagggttt catggtcaag ccgccgggat tcgatccgaa
     3781 caagaaatat ccgctaaagt ttttgatcca tggcgggccg caaggggcat ggggaaattc
     3841 gtggtcgtat cgctggaacc cagagttgtt cgcggcgaac ggctacgtgg tggtgatgat
     3901 caacttccac ggctcaaccg gttacggaca gaagtttacc gactcgatca gcggcgattg
     3961 gggcggcaaa tcgtatgtcg atctaatgaa ggggctcgat tacgtggaga aaacgtatcc
     4021 attcatagac aagaatcgcg aggccgcgct cggcgcgagc tacggcggtt acatggccaa
     4081 ctggattctc ggccacacga accgattcag gtgcatcgtg tcgcacgacg gcgtgttcaa
     4141 cgcggagtca gcttatggga cgactgagga gctttggttc cccgagtggg aattcaaagg
     4201 tccgccgtgg aaacagcgcg agttgtatcg gaaattttcg ccgcatctgt tcgcggacaa
     4261 attcaaaacg ccgacgctgg tgatccatgg tcagaacgat taccggctcg atgtctcgca
     4321 gggtttcgat ctttttacga cattgcaacg gctgaaagtg ccgtcggaaa tgttgtattt
     4381 tcccgacgaa ggtcactggg tgctgaagcc gcagaactcg cggctttggt acaagaccgt
     4441 gaacgattgg gtcgatcggt ggtgcaaata atccttgtca tgtcgaccga agtggagaca
     4501 tctctcaata tttctaatcg gaaacggtaa gagatttgat ttgctcactt cccgatcgct
     4561 cagcttccgg ccttcccatc tatctcgcgg cgtcccagcc gctcgattct cgactgcgct
     4621 cggaatgaca agaaaagcat gatcgcggag ttctgtttcc ggggagcaca cgccgctggc
     4681 gtgttcatct cggcagcttg ccgagatgtt tatccattca cacgaacagc tctcgcggag
     4741 ggtt
//
LOCUS       DASQUQ010000139         9112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1196341, whole genome shotgun sequence.
ACCESSION   DASQUQ010000139 DASQUQ010000000
VERSION     DASQUQ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196341"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(620..1924)
                     /locus_tag="VE486_03930"
     CDS             complement(620..1924)
                     /locus_tag="VE486_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HZA38269.1"
                     /translation="MPESLVDAVSNRQRVTGAPHDLYRYPARFAPVFAREAIQAFTKP
                     TDLVLDPFCGGGTTLVEALALGRRAVGMDINALATFLTRAKTTPISIHDKRAIATWIA
                     TLQSAERVCESDLPHVADPEIAYYHRNLPEAARIFFCWVLQQLTRLPTVRQQRFVRLA
                     LLSVGQWALDCKIRVPTAAELQAEFYHRLRDAIQDYFEFISGAAQRNQVPRCHLLSLR
                     RIINCSSQRSAEDGRILKSWLPAKLVLTSPPYPGVHVLIIAGKFSAAGKTPAPFWLAN
                     QRDGAGESYYLLGPRQENELATYFARLKSIFSSVRHLLNRDSLVIQLVAFSDPEWQLS
                     HYLRTMKDAGYKELEADCKTDNKIAGRIWRTVPGRRRRLVFAPIRFAHANPVRQLDAT
                     RSFALLSLCFPGRLSASAIPARRFYCRFNAKLKAVSKRLELS"
     gene            complement(2206..3282)
                     /locus_tag="VE486_03935"
     CDS             complement(2206..3282)
                     /locus_tag="VE486_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZA38270.1"
                     /translation="MIIFLRSRNAAPFELNGDGRFVMSRKDFVPAYVQAFEEGLLQKR
                     AEQAVESLRSCRVCPRDCEIDRFNNKIGVCKSGRLARVASAFPHFGEEDCLRGWNGSG
                     TIFFGWCNLRCVFCQNFETSQFGEGAEVTAAELARIMLDLQRIGCHNINFVTPEHVVP
                     QILEALVIAVERGLRLPLVYNTSAYDSIESIQWMDGLVDIYMPDFKFWDAEHCRKYLV
                     ASDYAAAARAVIAAMHAQVGELKVDEDGLALRGVLVRHLVMPALLDDTREIMCWIAEN
                     LSRDTYVNVMDQYYPAHKAETEPRFAEIRRGITDKEICGAFGLARAAGLWRFDTRWRN
                     VIPHGAPVWLPRMRQQNEPFANAN"
     gene            3537..4952
                     /locus_tag="VE486_03940"
     CDS             3537..4952
                     /locus_tag="VE486_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38271.1"
                     /translation="MAVKTEKELNETQRSHWLKSVAAIELRNFGYAISLLQGILKQEP
                     QFLTGRQLLRRTEVTKSKSAKKSFFNISTAPLAVMKAQRQIKKDPKHAIEMLEGVLEE
                     EPYNRQANLALKEAAVAAGWPEIGVFALRTLLEENPRDVKVLHELGRLYHELGESDRE
                     VEIYNQISEINPLDAEALRLGKDASAHASMKSGGWTEAESYRDLIKDKEAAISLEQQS
                     RMRLTGESLDQQIAETYARHKSDPTNIDLARRLGALNEQKEDFEAAVGWYQYAADLTK
                     GADAGLVRKVSDLKIKRLDREIAVDEEFLSTHDARHELYEKKSEGLKTAKRNRAEILL
                     VDARNRVERNPTDLQLRFELGEHLVNAEHFREALPELQRARQNPNARLKAMNLLGCCY
                     GELGMLDLATKELEEASKEILSMDVMKKEILYNLGLVYERMGEREKSLACMKQIYQAD
                     YGYKDVAKRVESSYKQNISQA"
     gene            5112..5627
                     /locus_tag="VE486_03945"
     CDS             5112..5627
                     /locus_tag="VE486_03945"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HZA38272.1"
                     /translation="MKTKLILISRFTILALIVASLSFAGLAQGQESPAATTSSLSAKD
                     KTFMKKAAKGGMMEVAMGKVAEQNGQSEDVKSFGKRMVTDHTKANDELKSIAEKKGVK
                     LPSKEPSEKWSSDKAYMDMMVKDHEKDLAEFQEQASTGTDPDVKKFAEDTAKMVQEHL
                     DLAKETQSKLQ"
     gene            5752..7155
                     /gene="pyk"
                     /locus_tag="VE486_03950"
     CDS             5752..7155
                     /gene="pyk"
                     /locus_tag="VE486_03950"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZA38273.1"
                     /translation="MRKTKIICTLGPATQQTDVMRQLIQKGADVFRLNMSHADQGWVR
                     EVVPCIRALAQEARRSVGILLDTQGPAIRTGDLEAPLHLKQDDILELTVAGARSEERY
                     SVGVNYEGLVDDVSVGDTILVDSGMIRLVVLEKRGKRIRCKVMTPGTVGSRRHINLPG
                     VHVNLPPLTKKDISDISLGVELGVDFVALSFVRQKSDLEQLRNVLVEKKSKALVIAKI
                     EDQLAVRAINDMIQGADVIMIARGDLGIECPMEELPIIQRRIVKSCLRHGKPVIVATQ
                     MLESMITNVLPTRAEITDVANAVFEQADAIMLSGETSVGRYPIECVRVLSRVALRIER
                     SGGAGYAESAILEGKRQKTVASAVFLANSLANSKIIVFTRHGAMAHNVAKLRPENAPI
                     FAFTPSEEVYRQLSMYWGVYPVKIDFIDDPNATIEAAVTFLRRAKLTAPGDELVIISD
                     LHVGEERIDSVQLRTTR"
     gene            complement(7241..8110)
                     /locus_tag="VE486_03955"
     CDS             complement(7241..8110)
                     /locus_tag="VE486_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HZA38274.1"
                     /translation="MKKSYMAAEDSDSGIKIYLREIGQIPLLTPDQEIELAAKIKKGD
                     REARALMIRSNLRLVVKIAHDYANLGLPLLDLISEGNIGLMKAVERFDPAKGGKLSTY
                     AAWWIKQSIKRALANQSKTIRLPVHLVDKISKMRRVSLQMSEELGREPTDEELGDEIG
                     IASGKVSQLKTVSIRPASLDAPISDDDSTEFGEIVGDEEAQTPFELLRDKNLRNEVGG
                     LLDVLDDREKKIIFQRFGLDGGKPKTLEEVGKKFGVTRERIRQLQNIALSKLRRALSK
                     KERPIEVELSVEA"
     gene            8412..>9112
                     /locus_tag="VE486_03960"
     CDS             8412..>9112
                     /locus_tag="VE486_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HZA38275.1"
                     /translation="MLAKIYSAAVYGVDAYEVEIEVNGAGGDPNIVIVGLPDAAVKES
                     RDRVTTAIANSGYYWPRGRTTINLAPADIKKEGPSFDLPIALGMIAVTEGLDSSPFEN
                     FSFVGELALDGTVRAVKGVLPVALEARRRGRRALFVPEANALEAAMVDKIDIYGVQNL
                     RQTFAFLRGEIALLPTRADLTKFFATHQIYDVDFSDVKGQGHVKRAIEVAVAGGHNVL
                     MIGPPGSGKSMLAKR"
BASE COUNT         2199 a         2422 c         2533 g         1958 t
ORIGIN      
        1 gaaccaacat tcccgacttc ctctccgctt gctgttagca gagagcgaat gactgctacg
       61 atctgcaccg ttgcatcacg cgattgaaga cgaacttgag gttctactga agagccatga
      121 aggcctgcgc gagctaaaag agcgcagacg gcgcgaggaa ctcgaggaaa gcctcaaaga
      181 ttcgaaaccg ctagaagatg tactggaaaa actcatccgg cactcaccca gcctgtcaca
      241 gctatttttg ctcggcgatc gcgccacaaa tccgtttaag ccaaagggtg tccagagcga
      301 agaaaaacga ttcgagggca agcgctatcc aacttacttc agattcaaag gcaaaccgta
      361 cggagaaaaa ttggagcggg actgccatat caacatgcgc gcgcgaatct ccttcgaaac
      421 agacgcggtg aacgactact tcagccgcca agtggatccg gggagctttg ccatcgatat
      481 gcttggtcca aacaccaaat cattcgcagt agaagactac agtctgaatc ttcagaacgg
      541 aattgctacg ctgaatttaa agctgccgag ccactgtgct gtggacgaaa agcgtgattt
      601 cgtggttacg gtgacggatt caactcagct cgagccgttt cgaaactgct ttcagcttcg
      661 cattaaacct gcagtagaag cgcctggcgg gaatggccga cgccgaaagc cgcccgggga
      721 aacagaggga gaggagcgcg aagctccgag tggcatccaa ctgccgaact gggttcgcgt
      781 gggcgaatcg aattggagca aacacgagcc gccgcctccg accaggcact gtccgccata
      841 ttctaccggc aattttgtta tccgttttgc aatctgcttc taattcttta tagcccgcgt
      901 ctttcatcgt tcgaagatag tgtgacaatt gccactcggg atcggagaaa gcgaccagtt
      961 ggataacaag ggaatcgcgg ttcaacaggt ggcgcactga cgagaatatt gatttcaggc
     1021 gagcgaagta cgtcgctagt tcattctcct gacgtggccc gagtaagtag tacgactcgc
     1081 cagcgccatc ccgctgattt gccaaccaaa acggtgcagg tgttttcccg gcggccgaaa
     1141 atttgccagc gatgataagt acgtggacgc cggggtaagg aggtgaagtc aatacgagct
     1201 tggctggcag ccatgatttg agaatgcggc catcctccgc ggatcgctgc gatgagcaat
     1261 tgatgatccg acggagtgat aaaagatgac agcgtggaac ttgattgcgc tgcgccgcac
     1321 ccgaaatgaa ctcgaaataa tcttgaatag catcacgcag gcggtggtag aactcagctt
     1381 gcaattcggc agcggtagga actctgattt tgcaatcgag cgcccattgc ccaacgctca
     1441 gtagagccag gcgcacaaat cgttgctgac gtactgtcgg caatcttgtg agctgttgaa
     1501 gaacccagca gaaaaatatc cgtgccgctt ccggaaggtt gcggtggtaa taagctatct
     1561 cgggatccgc aacgtgcggc aaatcagact cacacactcg ttctgctgac tgaagcgtcg
     1621 cgatccatgt agcgatcgca cgtttatcgt gaattgatat cggcgtcgtt tttgcacggg
     1681 taaggaatgt cgcaagtgca ttgatgtcca taccaacggc tcgacgaccg agtgcaagcg
     1741 cctcaactag cgtcgtccct ccaccacaaa acggatcgag cacgaggtcc gtaggttttg
     1801 taaaagcttg gattgcctcg cgggcgaata ccggagcaaa acgcgcagga taccggtaaa
     1861 ggtcatgcgg tgccccggtg actctttgcc ggttcgaaac cgcatcgacc aacgactcgg
     1921 gcaagcgaag acgcatagaa cctagcggcg tttcgttttc ggtgctcggc gtcgcttcga
     1981 tctaacccga ggtgcggcag caactgtgat ccgttcaccg ttcgtggtga tctccaacgg
     2041 tgtttctggc ttaattttca gtaaatccag aatcggccga tctatcagca atgcgaagct
     2101 gttgccatgt ttagtcaggt acttaatcat ccgctatgta agcacaatgg atacacattg
     2161 taatcccatt gtgacaacgc gcaaggcgtt aaccttatcc gtaaactaat tcgcgttggc
     2221 gaacggttcg ttttgctgac gcatgcgggg cagccagact ggcgcgccgt gcggaatcac
     2281 gttgcgccag cgggtgtcga accgccagag accggctgca cgcgcgagtc cgaatgcgcc
     2341 gcagatttcc ttgtccgtga tgccgcgcct gatttccgcg aagcgcggtt cggtttcggc
     2401 tttgtgcgcc gggtaatatt ggtccatgac attgacgtag gtgtcgcgcg agaggttctc
     2461 ggcgatccag cacatgattt cgcgcgtgtc atcgagaaga gcgggcatga cgagatgcct
     2521 aacgaggacg ccgcgcaatg cgaggccgtc ctcgtccact ttcaattcgc caacttgcgc
     2581 gtgcatagcg gcaatcacgg cacgcgcggc cgccgcgtaa tcgctggcca cgagatattt
     2641 tcggcaatgc tcggcgtccc agaacttgaa atcaggcatg tagatatcca ccagcccgtc
     2701 catccattga atgctttcga tgctgtcgta cgccgatgtg ttatagacga gcggcaggcg
     2761 caagccgcgc tcgactgcga tcacgagcgc ttcgagaatt tgcggcacga cgtgctcggg
     2821 cgtgacgaag ttgatgttgt ggcagccgat tcgttgcaga tcgagcatga tgcgcgcgag
     2881 ctctgctgcg gtcacttccg caccctcacc gaattggctg gtctcgaagt tctggcagaa
     2941 tacgcagcgc agattacacc agccgaaaaa gatggtgccc gacccgttcc agccgcgcag
     3001 acagtcttcc tcgccaaaat gcggaaacgc gctcgccacc cgcgcaagcc ggccgctctt
     3061 gcacacgccg attttgttgt taaaccggtc aatctcgcag tctcgtggac agacgcgaca
     3121 cgaccgcagc gattccacgg cttgctcggc ccgcttctga agaagtccct cttcgaacgc
     3181 ttgcacgtac gccgggacaa aatctttgcg tgacataaca aagcgtccgt cgccgttgag
     3241 ctcaaaaggc gcagcgttgc gcgaacgaag aaaaatgatc atgggcgagt ctcgcgattc
     3301 gtattctgcc aagattaaac gctcaacgcc caacgctcaa cgcccaatgc acaatcgggc
     3361 gacaactccg ctacttgtcc cgagcgaaga tccacgttga agagccgcag tctagtttgt
     3421 tgcgaagcag gtgtcctcgg caaggcaccg agaactgcac gcgaggcatg cgcgctcccc
     3481 agaattccgg tgacggcacg tggatgtgaa attatagtag cagtcggctg cgcattatgg
     3541 ctgttaaaac cgagaaggaa ctgaacgaaa cgcagcgcag ccattggctc aaatcggtag
     3601 cggcgattga gctgcggaat tttggttacg ccatctcgct cctgcaggga attctcaaac
     3661 aggaacctca gtttctcacg ggccgtcagc tgttgcgccg caccgaggta acgaaaagca
     3721 agtcggcaaa gaaaagtttt ttcaatatct cgactgcgcc gctcgcagtc atgaaagcgc
     3781 aacgacaaat caaaaaggac ccgaagcacg cgatcgaaat gctggaagga gttttggaag
     3841 aagagcctta caatcggcag gcgaatcttg ctttgaagga agcggcggtt gcggccggct
     3901 ggcccgagat tggagtgttc gctttgcgga cgttactcga agaaaatccc cgtgacgtga
     3961 aagtgctgca cgagcttggg cgtttgtatc acgagcttgg agaaagcgat cgggaagtcg
     4021 aaatttacaa tcaaatcagc gagataaatc cgctcgatgc ggaggcgctt cggttgggca
     4081 aagatgcgtc cgctcatgcc tcgatgaaga gcggcggatg gacggaggcg gagagctatc
     4141 gcgatctgat caaggataag gaggcggcga tttctctcga gcagcagagc cggatgcgat
     4201 tgaccggcga atcgctcgat caacagattg cggaaactta tgcgcgccac aaatcggacc
     4261 cgaccaatat cgatcttgcg cgacgcctgg gcgcactaaa tgagcaaaag gaggatttcg
     4321 aggcagcggt tggctggtat cagtatgccg ccgatctgac aaagggcgct gacgctggat
     4381 tagtccgaaa agtttccgat ttgaaaatca aacgcctcga ccgcgagatt gctgtggacg
     4441 aagaatttct ctccacgcac gatgcgagac atgagttgta cgagaagaaa tctgaggggt
     4501 tgaagaccgc gaaaaggaac cgggcagaaa ttctcctcgt tgatgcgcgc aaccgtgtag
     4561 aacgcaatcc gaccgatttg cagcttcgat ttgagcttgg cgagcatctc gtgaatgcag
     4621 agcattttcg cgaggcgctg cctgagttgc agcgcgcgcg acaaaatcca aacgcgcgtc
     4681 tgaaagcgat gaatctcctc ggttgctgct atggtgaact tggaatgctc gatctggcga
     4741 cgaaggaact cgaagaggcg tcgaaagaaa ttctatcaat ggacgttatg aagaaggaga
     4801 tcctctacaa cctcgggctt gtttacgagc ggatgggtga acgggaaaaa tcgcttgctt
     4861 gcatgaagca gatttaccaa gccgactacg gctacaagga tgtcgcaaag cgcgtggaaa
     4921 gctcgtacaa acagaatatc tctcaagcct gatccgctga acgctctccg tgttgaccgg
     4981 cagtcgcgag attaggcgct cgcgcccaag ctcaattccg actttgccac gtccaggcag
     5041 acgaggattt tcgattctta attgtcaaaa tttgccgggt ttgttccgac ggtttcaacg
     5101 gagaaaaaaa tatgaaaacc aaactcattc ttatttcacg gttcacgatc ctcgctctca
     5161 tagtcgcatc tctcagtttc gccggcctgg cgcagggaca agaaagtccc gcagccacaa
     5221 cctcgtcgct tagcgcaaaa gacaagactt tcatgaagaa agccgccaaa gggggcatga
     5281 tggaggtagc aatgggaaag gtggctgagc agaatggtca aagcgaggat gtaaaatcgt
     5341 tcggcaagcg gatggtcacc gatcacacca aggccaatga cgagctcaag tcgatcgccg
     5401 agaaaaaagg cgtcaagctg ccaagcaaag agccgagcga aaaatggagt tccgataaag
     5461 cttatatgga tatgatggtg aaggatcacg aaaaggattt ggctgaattt caggagcagg
     5521 ccagcacagg aaccgatccc gacgtgaaga aattcgccga ggacacggcc aagatggtcc
     5581 aggaacatct tgacctcgcc aaagagactc aaagcaagct gcaataggaa cacactgact
     5641 gcgcgataat gcggcacgcc tgaaaagacg tgccgcattt tctttgctat ttagaagtgg
     5701 attccaaaag cgccttgctt ttgctcggtc aaacttaatc ctcctggtgg gatgcgtaag
     5761 accaaaatca tctgcacgct cggtccggct actcaacaaa ccgacgtgat gcggcaatta
     5821 atccagaaag gggcagacgt tttccggctg aacatgagcc acgccgatca ggggtgggta
     5881 cgtgaagttg tgccgtgcat tcgcgcgctg gctcaagaag cccgccgatc cgtggggatt
     5941 ctgctcgata cgcaggggcc agccattcgc acaggagact tggaagcccc tctccatctt
     6001 aagcaagacg acatcctgga gctcacggtt gccggcgcca ggagcgaaga gcgctattcg
     6061 gtcggcgtaa attacgaagg gctggttgat gacgtttccg ttggcgacac cattttagta
     6121 gatagtggca tgatcaggct ggtggtcctc gaaaaacggg gaaagcggat ccggtgcaag
     6181 gttatgacgc ccggaacggt cggctcccgg cgtcacatta atctgcccgg tgtgcatgtg
     6241 aatctgccgc cgctcacgaa aaaggatatt tctgacattt cactcggcgt agagctcgga
     6301 gtcgattttg tcgccctgag ttttgtgcgc cagaagagcg atctggagca attgcgcaac
     6361 gtccttgtcg aaaaaaagag caaagccctg gtcatcgcca aaatcgagga tcaactggcg
     6421 gttcgcgcga tcaacgacat gatccaaggc gcagacgtca tcatgatcgc ccgcggcgat
     6481 ctggggatcg aatgcccaat ggaggagctg cccatcatcc agcgtcgaat tgtaaaatcc
     6541 tgtcttcggc atggtaagcc ggtcattgta gccacgcaga tgctcgaatc gatgatcact
     6601 aatgtgttgc ccactcgggc tgaaattacc gatgtggcca atgcagtttt tgagcaggca
     6661 gatgcgatta tgcttagcgg tgaaacaagt gtggggcgtt atccgatcga atgcgtccgg
     6721 gtgttgagcc gcgtcgccct gcgcattgag cgcagcggag gggccggtta tgcggaatcc
     6781 gcaattctcg agggcaagcg gcaaaaaaca gttgcttccg ccgtgttcct agccaattcg
     6841 ctggcgaatt caaagattat tgtattcacc cggcacggcg cgatggcgca caacgttgca
     6901 aaacttcgtc cggaaaacgc gccgatattt gcttttactc caagcgaaga agtctatcgc
     6961 caattgtcaa tgtactgggg cgtttatcca gtgaaaatcg actttatcga tgatccgaac
     7021 gcgacgatcg aggccgcagt aacgtttttg cgccgagcca agctgaccgc tccgggcgat
     7081 gaattagtga tcatcagcga tctacacgtg ggcgaagaac ggatcgattc cgtgcaactt
     7141 cggacgacac gataagaaag ctgagatcgc ttcgctctgc gaaccgcagg gctggcgtgt
     7201 cagcgcacca cgtcgctaac agagcgcgcc ggctgtgaaa ttaagcctcg accgacaatt
     7261 caacttcgat tgggcgttcc ttcttgctca gcgcgcggcg aagttttgaa agcgcgatgt
     7321 tctgcaactg ccgaatgcgc tctcgagtaa cgccgaattt cttgcccact tcctcgagcg
     7381 ttttcggttt gccaccgtca agcccgaatc gctggaaaat gatcttcttc tcccggtcgt
     7441 ccagcacgtc aagcaaaccg ccgacctcat tccgcagatt cttgtcgcgc aatagctcga
     7501 acggggtctg agcttcctcg tctccgacaa tctcgccgaa ctcagtggaa tcatcgtcgc
     7561 taatcggcgc atcgagcgaa gccggacgaa tcgacacagt cttcagctgc gagacttttc
     7621 cgctggcaat cccgatctcg tcgccaagct cctcatctgt cggttcgcga ccaagttcct
     7681 cgctcatttg gagcgaaacc cggcgcatct tggaaatctt gtccaccaga tgcacgggca
     7741 ggcggatcgt cttgctctgg ttcgccagcg ctcgcttgat ggactgcttg atccaccatg
     7801 cggcgtacgt gctgagcttc ccgcctttcg ccggatcaaa acgttcaacc gccttcatca
     7861 gcccgatatt tccttccgag atcaggtcaa gaagtggcag ccccagattc gcgtaatcgt
     7921 gggcgatctt gacgacgagg cgtaaattcg agcgaatcat cagcgctcgc gcttcgcgat
     7981 cgcccttctt gatcttcgcg gccagctcga tctcctgatc cggggtcaag agcgggattt
     8041 gcccgatctc acgcaggtaa atcttaattc cgctatcact atcttcagca gccatataac
     8101 tctttttcat taattaccag gggggtggga atcgacagtt tgccccaaat aaagttcgtt
     8161 tgggccttgt cgatcacgcg ttgtagcatt tccaaaagta tttaacaagg ttaaatattc
     8221 tgatttctga cagtccccgg acagctcttg ttcaatcttt ttccgccttt tgcaggccgg
     8281 ccatttaggc gatgttgtcc gagagaaatc gcccaataaa ttagacaaac tttacgaacg
     8341 gaagttgcat ctgccatagc gtgcatttgc cgcttttccg ctctgtgatt ttctgcgacc
     8401 ctgccaccgc gatgctggcc aaaatctact ccgcggcggt ttacggcgtg gatgcgtacg
     8461 aagtggagat tgaagtgaac ggcgcgggcg gcgatccgaa tattgtcatc gttggcttgc
     8521 cagatgccgc cgtgaaagag agccgcgacc gcgtgaccac ggctattgca aatagcggct
     8581 attactggcc gcgcggccgc accacgatca atcttgcccc cgccgatatc aagaaggagg
     8641 gaccaagctt cgacttaccg attgcgcttg ggatgatcgc ggtgaccgag ggattggaca
     8701 gttcgccctt cgaaaatttc agcttcgtgg gtgaactcgc tttggatggc accgtgcggg
     8761 cagtgaaagg tgtgctccca gtcgctttgg aagcacgacg ccggggcagg cgcgcactct
     8821 tcgtccccga agccaatgcg ctcgaagccg ccatggtcga taagatcgac atctatggcg
     8881 tgcaaaattt gcggcagaca ttcgcttttt tgcgcggcga aattgcgctt ctgccgaccc
     8941 gcgctgatct gaccaaattt tttgccacgc atcaaattta cgatgttgat ttcagcgacg
     9001 tgaagggcca gggccacgta aaacgggcca tcgaagtggc cgtcgccggc ggccacaacg
     9061 tgctgatgat tggtccccct gggtcgggca aatcgatgct ggccaaacgc at
//
LOCUS       DASQUQ010000140         7906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_936948, whole genome shotgun sequence.
ACCESSION   DASQUQ010000140 DASQUQ010000000
VERSION     DASQUQ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936948"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VE486_03965"
     CDS             <1..359
                     /locus_tag="VE486_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZA38276.1"
                     /translation="ASTSKSLAPEIVGEEHYNVARGVQRVLQRYKDLQDIIAILGMDE
                     LSPEDKLTVYRARKIQRFLSQPFHVAEVFTGTKGQYVSIEETVRGFKEILDGKHDDVP
                     EQNFYMKGNIDMIKEG"
     gene            435..845
                     /locus_tag="VE486_03970"
     CDS             435..845
                     /locus_tag="VE486_03970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZA38277.1"
                     /translation="MATLKLEIVTPETKIYSEDVDMVTLPGSEGELGVYPKHVPLLTT
                     LKPGELRVMQNGRETAMAIGEGFVEIKADAVSVLTDMALEAEKIDVAAAEAAVERAKA
                     AMKEDHTAEEVAAIQASLQKALAQLHVKRRRHHG"
     gene            944..1753
                     /gene="lpxI"
                     /locus_tag="VE486_03975"
     CDS             944..1753
                     /gene="lpxI"
                     /locus_tag="VE486_03975"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HZA38278.1"
                     /translation="MHIPDVLGIIAGNGVYPRLLADSARKTGVKKIVAAAFTDETDPA
                     LTQHVDRIEWMRVGQLNRLLKFFSEQKVHHAIMAGQIAPKNLFDLRPDWKALLLLGKL
                     KQRNAESIFAAIADELAKVNVDLLPAATFLEDSLAPPGLIAGAKLSRREEEDVDLGWK
                     IAREIARLDVGQTVIVKNGTILAVEAFEGTNEAIKRAGALASKGAVMVKVAKPNQDMR
                     FDVPVIGVETIRIAAEAKLRVIAVEGGRTLLLEKDAIADFAERAKVSIIAR"
     gene            1798..1870
                     /locus_tag="VE486_03980"
     tRNA            1798..1870
                     /locus_tag="VE486_03980"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1831..1833,aa:Ala,seq:ggc)
     gene            complement(1888..3243)
                     /locus_tag="VE486_03985"
     CDS             complement(1888..3243)
                     /locus_tag="VE486_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZA38279.1"
                     /translation="MITVWRADAGYEATRRRMVWNDRIPQRFPDVIVTVASDTDVIEA
                     VKLARSRGLCISIRAGGHSWLATSLRDGGMLIDLSRLNGVMVNAAARTATVQPAIKNT
                     ELVAALEPHRLAFPAGHCPTVAVGGYLLAGGQGWNQGSWGIACKNVLAIDLVNAEGEL
                     ITADAQQNSELLWAARGGGPGFPGIILRYHLRLFPRPKVIMQSIYVYPLELVEPVTTW
                     LSEIVPSLPSSVEQLLLLALPTPGVAAQLPSPRQRCLTLWSVAYGDSETETAAALAPL
                     ETCPVSNRMLVRQINTPVSWDDLFAIEAAVFPEGHRYDVGIIWSNAHPTPVLSGLRDR
                     LAQAPSLMTEILVAVTGPPAVDSSARDMAYSMAAPLYVGCYSVWDNADDDEVNEKWHH
                     ETLQSLEPITRGHYMGETDLLASPTRAADSLAPGVWERLQAVRRRYDPEGLFYGHIGQ
                     A"
     gene            complement(3253..3864)
                     /locus_tag="VE486_03990"
     CDS             complement(3253..3864)
                     /locus_tag="VE486_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZA38280.1"
                     /translation="MTNSKATNPADIESDEYKKQFLHEATRLAIESVEKGWGGPFGAV
                     IVRDGKIIGRGQNRVLLTGIPVYHAEVTAIIDACSRLNRKALLGSDYYEGMLEMIPRP
                     PGSPDPVPERAKMLKGCDLYVNGAPCPMCMSAIYWARIDRVYFGTRLSDTSKIGFDDE
                     FQYIDFRLPWEERKAIKCFPDFEREFALKACEAWQSKKDRHPY"
     gene            complement(3990..4751)
                     /locus_tag="VE486_03995"
     CDS             complement(3990..4751)
                     /locus_tag="VE486_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZA38281.1"
                     /translation="MLRFRHSGNLDAWRKSETWHNLVEDAQAFLAEKSSIEIEIKETE
                     PDGGVVEVIVTKVKPGREEAYRQWETKIQQAQSKFPGYRGSYVQPPIAGELGWTTLMR
                     FDSAEQLDRWLKSSERAALLREVEPLIDYAHLQRMETSFPGWFPTDPKTGQGPPNWKA
                     AMLVLLGLFPIVMLEARFLSPRLASLNSSFAMFIGNTISVALTTWLTMPLFIKGLGWW
                     LFPKSEASKIRVNIAGTALIFVLYAIEVAVLWNLL"
     gene            complement(4953..6326)
                     /locus_tag="VE486_04000"
     CDS             complement(4953..6326)
                     /locus_tag="VE486_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZA38282.1"
                     /translation="MLARKLPGEFYLAQILSVKANGIHASFLFAALGVVTLQNLLYAD
                     ERSVVETRSTGIVSEGISSNPGAVDIRTGTGELAETLKKLAGLPQESGVFLGGVWVGD
                     TNALLSGGAEPGKWSFNSLLIVGAALDANKLVGWKGGTFGIQFLQFNGQPTNQQAGIV
                     QGYNSLPGPEPLDRSELYQLWWRQELFDRKLVLRIGKVVPTNDFNNVLRPVPVQDQHL
                     AIPAVSGLLYTPVFINPTLLGAMPGYYNSAYGITTTFAPTRNVYFSSGIYDGNIARGV
                     QTGLTGPHFNGYYFNIWEAGAAWELAGMPGSFGAGGWRQTGRLSGPHNVTENGASGFY
                     LFGSQRFWRARPTIDNSGISGFAQFGVNNSATLPIDEYFGFGLTEFGLVPDRPNDSGG
                     IGLAWSSLNRNIFRRSSELMFQAYYQAHITHGAYFQPAISYIPTPGADPSLDAAWALT
                     LRVICLF"
     gene            6727..7407
                     /locus_tag="VE486_04005"
     CDS             6727..7407
                     /locus_tag="VE486_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZA38283.1"
                     /translation="MSARQSARTYNVQPVAELAKNDVVQPPLSPLTKSERILRPYDWQ
                     PKRDVPAEENVGGRGATIRNVPTGSKILQPPSDERAPREPPQSKPRHNWHGNKDHLSY
                     RDASRRHRQWRDRNWWKKNCETIVFVNSGYYFLDAGYWYPAWGYDPLNSYYDYDGPIY
                     TYGSLLPDQVIANVQEALQDAGYYFGPVTGSLSVETRAALANFQRDYGLIITGAIDQP
                     TVESLGLY"
     gene            7630..>7906
                     /locus_tag="VE486_04010"
     CDS             7630..>7906
                     /locus_tag="VE486_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZA38284.1"
                     /translation="MQQKEAQLRDGRIVRGEDPLNLEMPFEKLDSFITPSESFYVRTH
                     FPIPAIDKDAWWLRVEGEVEKPFAINYEELLELESLTIPVTLECAGNN"
BASE COUNT         1856 a         2211 c         2162 g         1677 t
ORIGIN      
        1 tggcatcgac atcgaaatcg ctcgctccgg aaattgtggg cgaagaacac tacaacgtcg
       61 cgcgcggcgt gcagcgcgtt ttgcagcgtt acaaagatct ccaggacatc atcgccattc
      121 tggggatgga cgagttaagc ccggaagata agctgactgt gtatcgcgcg cgtaaaatcc
      181 agcgcttcct aagccagccg tttcacgtag cggaagtatt caccggcacc aaaggtcaat
      241 acgtttcgat tgaagaaacg gtgcgcggtt tcaaggaaat tctagacggc aaacacgacg
      301 atgttcccga gcaaaacttc tacatgaagg gcaacatcga tatgattaag gaaggttaaa
      361 aaagttaaaa ggttacatcg ttaaatgctc cccgattctt ctgattcaac gatttaacaa
      421 tttaacgaat cgccatggcc actttgaaat tggaaatcgt cacgccggag acaaagattt
      481 actccgaaga cgtggacatg gtcacgttgc ccgggtcgga aggcgagctc ggggtttatc
      541 ccaaacatgt cccgttacta accacattga agccaggcga attgcgcgtc atgcagaacg
      601 gacgcgaaac ggcgatggcc attggtgaag gcttcgtgga aatcaaagcc gacgcggtct
      661 cggttctgac cgacatggcg ttggaagctg agaagatcga tgttgcagcg gccgaagctg
      721 cggtcgagcg ggccaaggcc gcgatgaagg aagatcacac tgccgaagaa gtcgcggcga
      781 tccaggcgtc gctccaaaag gcgttggccc aattgcacgt caaacgccgc cggcaccatg
      841 gataattttt ctgggtagcg cacgcgtccc gcgtgtatcg gcccgtccgg ctcggacttt
      901 ctgcgtctcg caaaagcaag cttccttctg ctagattcct cttgtgcaca taccagacgt
      961 tctcggaatc attgctggta acggcgtgta tccccgcttg cttgccgatt cggcgcgaaa
     1021 aacgggcgtt aaaaaaattg ttgctgcggc gttcacagat gaaaccgatc cggcgctaac
     1081 gcaacatgtc gatcgaatcg aatggatgcg agtcggacaa ctgaatcggc tcctcaaatt
     1141 ctttagcgag caaaaggttc atcatgccat tatggccggg cagattgcgc cgaagaatct
     1201 ttttgatttg cggccggact ggaaggcgct gctcttgctg ggaaaactca aacagcgcaa
     1261 tgccgaatcg atctttgctg cgatcgcaga tgaactggca aaagtgaatg tcgatctttt
     1321 gccggccgcg acatttctgg aagattctct tgcgccgccg gggttgattg caggagcgaa
     1381 actttcgcga cgcgaagaag aagacgtcga tcttggctgg aaaatcgcca gggaaatcgc
     1441 gcgcctcgac gtcgggcaga ctgtgattgt gaaaaatgga acgattctcg cggttgaagc
     1501 tttcgaaggc acaaacgagg cgatcaagcg cgccggcgcg cttgcgagca aaggcgccgt
     1561 catggtcaaa gtcgcgaaac cgaatcagga catgcgtttc gacgtgccag taattggcgt
     1621 cgaaacgatt cgcattgctg ccgaagcgaa attgcgcgtc atcgccgtcg aaggtgggcg
     1681 caccctactg ctcgagaaag atgcgatcgc cgattttgcc gaacgcgcga aggtttcgat
     1741 catcgcccgc tagttgcaat ccgcaatccg gaatctacaa tctacaattc ttcaaccggg
     1801 gtcttagctc agttggtaga gcgcctcaat ggcattgagg aggtcagggg ttcgaatccc
     1861 ctaggctcca taatgtttcg cgaaatctca cgcctggccg atgtggccgt agaacaatcc
     1921 ttcgggatcg tatcgacgcc gaaccgcctg tagccgttcc cagacgcctg gcgcaagcga
     1981 gtcggcggcc cgagttggag aagccagcag gtcagtctct cccatgtagt gtccgcgcgt
     2041 gatcggttcg agcgactgta gcgtctcgtg atgccacttc tcattcactt cgtcatcatc
     2101 ggcgttgtcc cagacgctgt aacaaccgac gtagagcggc gcagccatcg agtacgccat
     2161 gtcgcgggct gacgaatcga cggccggtgg cccagtcacg gccacgagta tctcggtcat
     2221 gagagatggg gcctgcgcca atcggtcccg caacccactc agcacaggag taggatgagc
     2281 gttcgaccag ataatcccca catcgtaacg gtgcccttcc gggaaaacag ccgcttcgat
     2341 ggcaaacagg tcgtcccatg atactggcgt gttgatctgc cgcaccagca ttcggttgct
     2401 taccgggcag gtctcgagcg gcgcgagtgc cgcggcggtc tccgtttccg agtcgccgta
     2461 cgccactgac cacaaggtga gacatcgttg ccgcggactc ggcaattggg cggccacgcc
     2521 tggcgtaggc aacgctagca gcagaagctg ctccaccgaa cttggcagtg acggcacaat
     2581 ctcggacagc cacgtcgtca ctggctcgac cagctccagc ggatacacgt aaatgctctg
     2641 catgataact ttcggacgcg ggaacaaccg caagtggtag cgcaggatga ttcctggaaa
     2701 acctgggccg ccaccgcgcg cagcccagag cagctctgag ttctgctgcg cgtcggccgt
     2761 gatcagttcc ccctccgcgt ttaccaaatc aatggccagt acgttcttgc aggcgattcc
     2821 ccaactgccc tggttccagc cttgtccgcc agccagaagg tagccgccga cagcgactgt
     2881 cgggcaatgc cccgctggga aggcgagccg gtgcggctca agcgcggcga ctagctcggt
     2941 attcttaatg gccggttgaa ccgtggccgt gcgagctgct gcgttcacca tcacgccgtt
     3001 gagccgcgag aggtcgatca gcatcccacc gtcgcgcaac gaagttgcga gccagctatg
     3061 gccgcctgcg cgaatcgaga tgcataatcc gcgcgaacgc gcgagcttga ccgcctcgat
     3121 cacgtctgta tcagacgcga ccgtgacgat gacatctggg aagcgctggg ggatgcgatc
     3181 gttccagacc atgcgacgtc gcgtcgcctc atagccggcg tcagcacgcc acaccgttat
     3241 catttcgcag gtttagtacg gatgccggtc cttcttactc tgccaggcct cgcatgcttt
     3301 cagggcgaat tcgcgttcga aatcgggaaa gcatttaatg gcctttcttt cctcccatgg
     3361 aagtctgaaa tcgatgtatt gaaactcgtc gtcaaaaccg atcttgctcg tgtccgataa
     3421 tcttgtgccg aaatacaccc gatcaatccg cgcccaatag atggcgctca tgcacatcgg
     3481 gcagggcgcg ccattcacgt aaaggtcgca acctttgagc atcttcgctc tctcagggac
     3541 cgggtcggga gagcctggag gccgaggaat catttccaac attccttcat aatagtccga
     3601 gccgagaagg gctttgcgat taagacgaga gcaggcatcg atgatggcgg tgacctcggc
     3661 gtgataaacc ggaatcccgg ttaaaaggac ccggttctgg ccccggccga taatcttgcc
     3721 gtccctgact atgacggctc cgaagggtcc tccccatcct ttctcgactg actcgatggc
     3781 cagccgcgtc gcttcgtgga ggaattgttt cttatactcg tcgctttcaa tatctgctgg
     3841 gttcgtggct ttgctgttgg tcatgcgcgg agtgtgacga tcgcgctttt aacaatgcaa
     3901 gcaatttctc ggaccgagcg ctgagttaat cttatgagaa cgtcgagatc gaatccgatc
     3961 gagttcgaat ccgaggatga acgcctggtt taaaggagat tccatagcac ggcgacttcg
     4021 atcgcgtaga gaacaaaaat gagcgcggtt cccgcgatgt taacacgaat ttttgacgcc
     4081 tcggatttcg gaaaaagcca ccagccaagg ccctttatga acaacggcat ggtgagccat
     4141 gtggttaacg ccacgctaat cgtgttgcca atgaacatcg cgaacgatga attcaggctc
     4201 gcgagtcggg gactgagaaa acgggcctcc agcatcacaa tcgggaaaag tcccagcagg
     4261 acgagcatcg ccgctttcca attcggcggg ccttgaccgg tctttggatc agtcggaaac
     4321 catccgggaa acgaagtttc cattcgctgc aagtgcgcat aatcgataag cggctccacc
     4381 tcccgcagca atgcagcccg ctccgaggac ttcagccatc tgtcgagttg ctccgcgcta
     4441 tcgaagcgca tcaacgttgt ccaacccagc tcaccggcaa tgggtggttg cacataggaa
     4501 ccgcggtaac cgggaaattt cgattgcgcc tgctgaattt ttgtctccca ctgccgatac
     4561 gcttcttccc tccctggctt tactttcgtg acaattacct cgacgacgcc cccatcgggc
     4621 tcggtctcct ttatttctat ttcgatggaa gatttttctg cgaggaaagc ttgcgcatct
     4681 tcgaccaaat tgtgccacgt ctccgatttc cgccaggcgt cgagatttcc gctgtggcga
     4741 aagcgaagca taaccgtcca cgctaacgtt tcggcgctgg caggcgtcat ttccgaattc
     4801 aagaaacctt cgaatcccgc cgcgagtgtc agagcctttg cctgccaggc agcgaattcg
     4861 ttttcggctc cgtgtcggag gtgaaatttc gcaagaagga ttgccgcgct tggcgatggc
     4921 gaagaaggat ccatggcggg caaaacaatg cactagaaga gacaaatcac tcttaaagtc
     4981 aatgcccaag cggcgtcgag actgggatcc gcgcccggag tgggaatgta ggaaatcgcc
     5041 ggttgaaaat acgccccgtg agtaatgtgc gcttgatagt aagcctgaaa catcaactcg
     5101 ctgctgcgcc gaaaaatatt ccggtttaac gacgaccacg ccagaccgat gccgccggaa
     5161 tcatttggcc ggtctggaac caggccgaat tctgtaagac caaaaccaaa atattcatcg
     5221 atcggaagcg ttgccgaatt gttaactcca aattgtgcga atccgctgat tccgctgtta
     5281 tcaatagttg gacgcgcgcg ccagaatctt tgcgaaccaa atagataaaa accggacgcg
     5341 ccattctccg tgacgttgtg cggaccgctc agccgtcccg tctgccgcca gcctcccgct
     5401 ccaaacgatc ccggcattcc ggccaattcc caggcagcgc ccgcttccca gatgttgaag
     5461 taatagccgt tgaagtgcgg acctgtcagc cctgtctgaa cgccacgggc aatattgcca
     5521 tcgtaaattc cagacgaaaa atacacattt cttgtcggag cgaaggttgt ggtgattcca
     5581 taagcggaat tataatagcc gggcatggcg ccaagcagcg tcggattgat aaagactggc
     5641 gtgtaaagca aacccgaaac tgccggaatc gccagatgtt gatcctgcac cggcacaggt
     5701 cgcaaaacat tattgaaatc gttagtcggc acgaccttgc cgatccgcag aacaagcttc
     5761 ctgtcgaaaa gttcctgcct ccaccaaagc tggtaaagct cggagcggtc gagcggctcc
     5821 ggccctggta gagaattgta accctgcaca atgcccgcct gctgattggt gggctggccg
     5881 ttgaattgca aaaattgaat cccgaatgtt ccacccttcc atcctacaag tttgttcgca
     5941 tcgagggcgg ctccgacaat gagcagactg ttaaaactcc atttccctgg ttcggccccg
     6001 ccggagagaa gagcatttgt gtcaccgacc cacaccccgc cgagaaaaac acctgactcc
     6061 tgcggcaatc ccgcaagttt cttcagtgtt tcggcgagct cgccagtgcc cgtgcgaata
     6121 tcgaccgcgc cgggattcga gctgatgccc tcagaaacaa tgcccgtgct cctcgtttcc
     6181 accaccgatc gctcatcggc atagaggaga ttctgcaacg tcaccacacc aagggccgcg
     6241 aagagaaacg aggcgtgaat gccgtttgct ttaacgctca aaatttgcgc tagataaaac
     6301 tcgcccggca gcttacgagc gagcaaaaaa ttggtcgaat gctgattagc ttcgaagaag
     6361 ttttcgggcg aaccttgcaa caaagaggca tttatgatgt ttaatcagcg gagtaagctt
     6421 atgaggcgac acacgacctt cacgctttgt ttgatttcag ccctcgcgat cgccgctccc
     6481 gccgtcgatg gacaaacaaa cgcacctgtc gctaaaaata gttcgaacgg gacagcgaac
     6541 ggtaggtccc acgtcacgag cacgcgtcca aacgcgccgg cagctcgcaa tgtcgctcgc
     6601 ggtccaggaa gcgttggccc ccgaatggcc tattcgcgcc cgacccaaac aggtgctcaa
     6661 cgtgcagtga atttgcggcc gaattattcc gcgccggccc gccccctgag ccctaccctg
     6721 gcagcaatga gtgctcgcca gagtgcgcga acttacaatg tccagccagt cgcagagcta
     6781 gcgaagaacg acgtcgtgca gcctccgctc tcgccgctca cgaagtctga acgcatcctc
     6841 agaccttacg attggcaacc gaaaagggat gttccagcag aagagaatgt aggaggccgc
     6901 ggcgccacaa tacgaaacgt tccgacggga agtaaaattc tccagccacc ttctgatgag
     6961 cgagcgccgc gggagccgcc gcagagcaaa ccgcggcata attggcacgg caacaaagac
     7021 cacctcagct atagggacgc atctcgccgc catcggcaat ggcgcgaccg caattggtgg
     7081 aagaaaaatt gtgagaccat cgtttttgtt aacagcggct attacttttt agatgcgggc
     7141 tattggtatc ctgcctgggg ttatgatccg cttaacagct attacgatta tgacggcccg
     7201 atttacacgt acggtagtct gctgcccgat caggtgatcg cgaatgttca agaggcgttg
     7261 caagatgctg gttactactt tggccccgtc acgggttcgc taagcgttga gacacgagca
     7321 gcccttgcga atttccagcg cgattacggc ttgattatta ccggcgcaat cgatcagccg
     7381 actgtcgagt cgctcgggtt gtattaggcc tgtctcacaa attctgtctc cgccgtgccg
     7441 tttcgaggcg ttcaagggaa agcacggcga ccgcgccatg gctacaccaa agcgttcccg
     7501 ctggtcgcct gaaatcagga ttcacttgat tgccgggcgt atgacgcttt acagaattac
     7561 atatgagcga tttcacccgg agagggcggg gatcatgcga caacgtcaag taaaaccccg
     7621 ggcgtaacga tgcagcaaaa ggaagcgcaa cttcgcgatg ggaggattgt gcgcggtgaa
     7681 gacccgctca atctcgagat gccattcgag aagctcgata gttttattac gccatcagaa
     7741 tcattttatg ttcgaacgca tttcccgatt ccggcaatcg ataaagatgc gtggtggttg
     7801 cgcgtggagg gcgaagttga gaaaccattt gcgatcaatt acgaggagtt gctcgaatta
     7861 gaatcgctga caattcctgt gaccctggaa tgcgcaggta acaatc
//
LOCUS       DASQUQ010000141         4908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_118956, whole genome shotgun sequence.
ACCESSION   DASQUQ010000141 DASQUQ010000000
VERSION     DASQUQ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118956"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VE486_04015"
     CDS             <1..567
                     /locus_tag="VE486_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38285.1"
                     /translation="ARELSELARTFLRRAGDQLYVGIYYSRWLIEQLEQHDPRAGLSS
                     HNIRSLIMFVEEINHALHAALQFKNGAREIGSEEFARNLELQAQIDTYLVLLLFVAFF
                     RKAQRISQTDRRWLRFHLFVRQCPEAFRDKNLRARYLETTQLAASYTHYLDTLNGQRR
                     LDEIRRFRSLDYDAKRDRILALTDGNEN"
     gene            648..1397
                     /locus_tag="VE486_04020"
     CDS             648..1397
                     /locus_tag="VE486_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38286.1"
                     /translation="MVRPWPIFLVIALGCGLGGCERAPQTVDEVIERNRQAMAGRATI
                     EAVNSIEVDLHIVDPGFEVDGNYRAARPGRMRIDVMAGGKHVYTEAFDGHRAWQWKGE
                     GAAIQEESPKATAALRHGVELPGKLFGLHELQRRGHRVDLTGRERIDGIDYCALRITL
                     NDGYTTTLYVDPKSWLVTRRRDFRPLHVDVDPTPTTIESRFSDFRKVNGVLFAFASSD
                     TDLTTDKVLETTTVRSIKVNPQFDPAIFRRL"
     gene            1456..1899
                     /locus_tag="VE486_04025"
     CDS             1456..1899
                     /locus_tag="VE486_04025"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HZA38287.1"
                     /translation="MYTYPKSPKEKTSGMIYFPRMLDKIRLQAGGELHQDYHKNLGAR
                     RAVDGVCCNFLRIKYADLRDRVLQGGADEDILEWCFEKGRRLNEGDLFVWNGFVSKLG
                     WRDFASSMLEEAKKKAGIPDRDDIATIPDLIDFDEDRFSAEGKAP"
     gene            1942..2376
                     /locus_tag="VE486_04030"
     CDS             1942..2376
                     /locus_tag="VE486_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38288.1"
                     /translation="MCLCILIFDACQYEVPITPKPTRKVEERLLGDWVSADGRENMKT
                     RRLDDDIYIVYYDGDLFRAYHSDIAETPFVSVQDINSSDRKYAYVIWKLSDDGKSLSL
                     RSVSNQVIPKETKDSVAVAALLKKNAGNPELFGEEIQFNKVK"
     gene            2550..3962
                     /locus_tag="VE486_04035"
     CDS             2550..3962
                     /locus_tag="VE486_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZA38289.1"
                     /translation="MNAQRTPLAAPNASPSPSPTPAVFSPQTLAELKRLQQAALSSNY
                     AYKQVAHLANNIGPRLSGSAQAAKAVEYVASELRAIGCEVQLEKVMVPHWVRGEETAA
                     LVQFPGQAENTTQKIVLCALGASVATPADGIIAELIAVRNFDELKALPRDKVAGKIVL
                     FNYPFDKQMAAEGRGGEAYGQAVVYRGDGPSAAARQGAVACLIRSVGGADYRLPHTGQ
                     TNYAQDVPKIPAGAVTAEDADLIADLVRQGPVKMKLILTPQQLPDVESYNVIGDIKGS
                     EHPEQVIIVSGHLDSWDLGTGAIDDAAGVAVSMEAANLIQKLHLKPKRTIRVIAWMNE
                     ENGLAGSKQYGKDYAKEMSNHFAAMETDGGAGHPLGINIRGKPEVKKMLAPVAAILQE
                     SGAGMLNFVEHCGADIEPLEKAGVPAFSPIQDSRFYFNYHHTAADTLDKIVPKELAEN
                     SAVIAVLAYALANMEQPLPR"
     gene            4031..4633
                     /locus_tag="VE486_04040"
     CDS             4031..4633
                     /locus_tag="VE486_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38290.1"
                     /translation="MSRAGRWVAELVLVFVGVYAAFWLSNYQQRQQDVERRDRILAFI
                     EQTLREGIESSKTNRAKEQEPEAAEFRRALDAGEMPPLHPFVFITDYSTSDLATMLQS
                     GGVQLLDVQTLRALRNDEAVIRWGLARMARYQKLSDELIVPNLDRDISFFYDPATKKL
                     RKQFEIYPQALEARVKFANELEQTHTELLKQIQAERRQNR"
     gene            4635..>4908
                     /locus_tag="VE486_04045"
     CDS             4635..>4908
                     /locus_tag="VE486_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38291.1"
                     /translation="MISTIRIFAFLLSILAASFAAAADYPAPIEGDYTIPDFKFASGE
                     TLPELRLHYRTLGKPEKDAQGKTTNTVLIMHGTTGSGAQFIRPEFAG"
BASE COUNT         1161 a         1294 c         1424 g         1029 t
ORIGIN      
        1 gcacgcgaat tgagcgagct tgcgcgcaca tttctacgtc gcgccggaga tcaactttac
       61 gtcggtatct attattcgcg ctggctcatt gaacaactcg aacagcacga tccgcgcgcc
      121 ggattgagca gtcataatat ccggtcgctg atcatgtttg tagaagagat caatcacgcg
      181 ttgcacgcgg cgcttcagtt taagaacggt gcgcgtgaga tcgggtcgga agagtttgcc
      241 cgcaacctcg agctccaagc gcagatcgac acgtatcttg tgctccttct gtttgtcgca
      301 ttttttcgaa aagcgcagcg catttcacaa acggaccgac gctggctgcg ttttcatctc
      361 tttgtccgtc aatgccccga ggcttttcgc gacaaaaatc tccgggcccg ttatctcgag
      421 acaacccagc ttgccgcgag ctacacgcat tatctcgaca cactcaatgg tcagcgacgc
      481 ctcgatgaaa tccgacgttt ccgttctctc gattacgacg cgaaaagaga tcggattttg
      541 gcgctaacgg acggcaacga gaactagggg aataccggcg cgcgcaagtc cgacgcggat
      601 taagagacaa gttgcgccgg cggcggcgtc tgttctatgc aatgagcatg gtacgtccct
      661 ggccgatatt cttagtcatc gcactcggct gcggattggg cggatgcgag cgcgctccgc
      721 agaccgtcga tgaagtaatt gagcgaaaca ggcaagcgat ggccgggcgg gccacgatcg
      781 aagcggtaaa ctcaatcgaa gtggatttgc atattgttga tccaggcttc gaagtggacg
      841 ggaattaccg ggccgcccgg ccgggccgaa tgcgaatcga tgtgatggct ggcggtaaac
      901 atgtttacac cgaagcgttc gatggacacc gcgcctggca atggaaaggc gaaggagccg
      961 cgatccagga agagtcgccg aaagcgaccg cggcactgcg gcacggagtg gaactccccg
     1021 gcaaactttt cggtctccat gagttgcagc gacgcggcca tcgcgttgat ctgactgggc
     1081 gcgagaggat cgatggcatc gattactgcg cgctgcgcat cacactgaat gacggatata
     1141 caacaacgct ttatgtggat ccgaagagct ggttggtcac gcggcgtcgg gactttcgac
     1201 cgctccatgt tgatgtcgat ccgacgccaa cgacgatcga atcacgcttt tctgatttcc
     1261 ggaaagtaaa tggcgttctg tttgcctttg ccagctcgga taccgatctg acgaccgaca
     1321 aggtcttgga aacaacaacc gtgcgctcta tcaaagtgaa tccgcagttc gaccccgcga
     1381 tcttccgccg cctgtagatt actatttttc tggggaattt tgccggtaag tcgcggcgcg
     1441 actaagttcg cgcgcatgta cacttatccc aagagcccga aagagaaaac gagcgggatg
     1501 atttattttc cgcgaatgct ggacaagatt cgcctgcagg ccggcggcga gttgcaccag
     1561 gattatcaca aaaaccttgg cgcgcgcaga gcggtggatg gcgtatgctg taatttcttg
     1621 cggataaagt acgccgatct tcgcgatcgc gtcctgcaag gcggcgccga cgaagatatt
     1681 ttggaatggt gtttcgaaaa agggcgacgg ctcaacgaag gagacctctt cgtctggaat
     1741 ggtttcgtct cgaagctggg ttggcgcgat ttcgccagct caatgctgga agaagcgaag
     1801 aagaaggcag ggattcctga tcgcgatgat atcgcgacca tcccggactt gatcgatttc
     1861 gacgaagacc gattctccgc ggaaggcaag gccccatgag tttatcaaat tcgatttgtc
     1921 cgagtaatct ctggcgttgc catgtgtctg tgcatcctga ttttcgacgc ctgccaatac
     1981 gaagttccaa tcacgccgaa gcccactcgc aaagtcgagg aacggcttct cggtgactgg
     2041 gtttccgcgg acggcagaga gaatatgaaa acgcgacggc tggatgacga tatctacatc
     2101 gtttattacg atggcgattt gttccgcgcc tatcactccg acattgcgga gacaccgttt
     2161 gtcagcgtcc aggacattaa ctcaagcgac cgcaaatatg cttacgtgat ttggaagctg
     2221 tcggatgatg ggaagagcct gagtttacgg agcgtcagta accaagtgat ccccaaggaa
     2281 acgaaagact cagtcgcggt tgccgcgctt ttaaagaaaa acgcgggcaa ccctgagctg
     2341 ttcggtgagg aaatccagtt taacaaagtg aagtagctgc cgcttggtgc ggtttcgcgg
     2401 aagccaggaa aattccggac cggcgcaccc acaaccgcaa cgattgatgg tcgcccgctt
     2461 ttctgacaga gtccggaatg tctgaatcga aaaagcgatt ttgcgcgatc tgtcttttct
     2521 cgcttccatt atttctttgt gtcccgcgaa tgaacgcgca gcgaacgccc ctggcagcgc
     2581 cgaacgcgag cccttcgccg tcaccaacgc cggctgtctt ttcgccgcag acactggcag
     2641 aattgaaacg gctgcagcag gcagctttga gtagcaatta cgcgtacaag caggttgcgc
     2701 atctggccaa caatatcggg ccgcggttga gtggatcggc gcaggcagcc aaagccgtgg
     2761 aatacgttgc cagcgaattg agagccatcg gctgcgaagt gcagctcgaa aaagtaatgg
     2821 tgccgcattg ggtgcgcggt gaagaaaccg cggcgctcgt tcaatttccg ggacaggcgg
     2881 agaacaccac gcagaagatt gtgctctgtg cgcttggcgc aagtgtcgcg acgcccgcag
     2941 atggaatcat cgccgagttg atcgcggtga gaaattttga cgagctcaag gcgctgcctc
     3001 gggacaaagt ggcgggcaag atcgtgctct tcaattaccc attcgataaa caaatggccg
     3061 cggaagggcg tggtggagaa gcgtacggcc aagcggttgt ttatcgcggc gatggtccga
     3121 gcgcggccgc gcgacagggc gctgttgcgt gtttgattcg gtcagtcggc ggcgcggatt
     3181 accggcttcc acacaccgga caaacgaact acgcgcaaga tgtcccaaaa attccggcgg
     3241 gcgcggtcac ggccgaggat gcagatttaa tcgccgattt ggtccggcaa ggaccggtga
     3301 aaatgaaatt gatcctgacg ccgcagcaat tgccagatgt cgagagctac aacgtcattg
     3361 gtgacatcaa aggcagcgag catccagaac aagtgatcat cgtttccggg catttggact
     3421 catgggatct gggcacgggc gcgatcgatg atgctgcggg tgtcgcagtt tcgatggaag
     3481 ccgcgaatct gatccaaaaa cttcatctca aaccgaaacg cacgatccgc gtcatcgcgt
     3541 ggatgaatga ggagaacggc ctggctggat caaagcagta cgggaaagat tacgcaaagg
     3601 agatgtcgaa ccattttgcg gcgatggaaa ccgatggggg cgccggccat cctctcggca
     3661 tcaacatcag gggaaagcca gaagtaaaaa agatgctcgc ccccgttgcg gcgattttac
     3721 aggagtcagg cgcgggcatg ctgaatttcg tcgaacactg tggcgcagac atcgagccgc
     3781 tggagaaagc cggcgtaccg gctttcagtc cgattcaaga cagccgtttc tatttcaatt
     3841 atcaccacac ggctgccgac acgctggata agatcgtgcc gaaagaattg gcggaaaatt
     3901 ccgctgtcat tgcagtgctt gcctatgcgc tcgcgaacat ggaacaaccc ctgcctcgtt
     3961 gagaattgta gacgtgatta taacgccaga accgatgagc accgtgactg ttgatcgcag
     4021 aaagccaatt atgtcccggg ctgggcgctg ggtcgcagag ctggtcctgg tgttcgttgg
     4081 tgtttacgcg gctttctggc tcagtaatta tcagcagcgc cagcaggacg tagaacggcg
     4141 cgaccgcatc ctggcgttca tcgagcaaac gttgcgcgaa ggaatcgaga gctcaaaaac
     4201 taaccgcgcg aaggaacaag aaccagaagc ggcggagttt cggcgcgcct tggacgccgg
     4261 tgagatgccg ccgctgcatc cctttgtttt catcacggac tacagcacaa gcgacttagc
     4321 cacgatgctg caatccggcg gtgtccagct gctcgacgtg cagacgctca gggcgctgcg
     4381 caacgacgaa gcggttatcc gctggggatt ggcccgcatg gcgcgttatc aaaaattaag
     4441 cgacgaactg atcgtgccga atctcgaccg ggacatttcc ttcttttacg atccggccac
     4501 aaaaaaattg cgaaaacagt tcgagatata tccccaggct ctggaagcaa gagtgaaatt
     4561 cgctaacgag cttgaacaga cccacactga gctcctcaaa caaatccaag ccgaacggcg
     4621 acaaaatcgt taacatgatt tcaacgatcc gaatcttcgc atttctcttg tccatcttgg
     4681 ccgcgagttt cgcggcggcg gctgactatc cggcgccgat cgagggcgat tacactattc
     4741 ccgatttcaa gttcgcgtcg ggcgagacgt tgccggagtt gcgcctccat tatcggacgc
     4801 tcggaaaacc ggagaaagat gcgcagggca aaacgaccaa taccgttctc atcatgcatg
     4861 ggacgactgg cagtggcgcg caatttatcc ggccggagtt tgccgggg
//
LOCUS       DASQUQ010000142         8347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1197175, whole genome shotgun sequence.
ACCESSION   DASQUQ010000142 DASQUQ010000000
VERSION     DASQUQ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197175"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(729..1691)
                     /locus_tag="VE486_04050"
     CDS             complement(729..1691)
                     /locus_tag="VE486_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZA38292.1"
                     /translation="MKPFEYVTAQTPQSASELIGGSGRYIAGGIDILGELKDYLFETK
                     RLVNIKSLPDTRAIKTGPQSWSIGANVTVAELAAHAEMRKTFPGLAEAAAEVASPQIR
                     NVATLGGNLAQHSRCWYYRHRDVRCLKEGGSTCYAREGENKYHSLFSGNPCISPTPSN
                     LAIALAALEATVAVQRSGKVVTLSIPSLYENAWSNPLAHNSLAPADLILRVEVPVRQT
                     RSAYLQVSEKHDFDWALVSCSAAARVDGRKLSGARVVLGQVAPVPYRSDEADKFLEDK
                     SLDDRVAAQAADLLLKGATPFEHNAYKVPLAHALIRRTLFKLIS"
     gene            complement(1688..3856)
                     /locus_tag="VE486_04055"
     CDS             complement(1688..3856)
                     /locus_tag="VE486_04055"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZA38293.1"
                     /translation="MSDWPKEIKYLGKPAPRIDGPAKVSGAAKYAYDVQPTGWLWGAI
                     LRSKWAAAKITSINLEKARAAPGIKAAVLAREGERQVRYYGEELAAIAGTSKEACLDA
                     LTLIEVQATPLPFVVKELDAMKPDAPKIFQDGANVGEAQVREDGNVAEGFSKSAAIIE
                     NTLETQVQLHHALETHGNTVSFTPEEITVWASTQGIFSVRDGMADNLDVSQSKVRVIC
                     DYMGGGFGSKFGPGLEGGLAARLSEAARAPVKLMLTRFDEALAVGNRPSSFQKVKLGA
                     AADGTLLAYELDSFGCPGFAAGGATAGGGSGATFPAPYIYKIPNTRVKQASVAINAGS
                     SRAFRAPGHPTASFGMESMMDDLAVKLGIDPVDIRIKNDPFEIRRKEYEIGREKFEWK
                     KKYRKPGSSSGIVKTGVGCAGATWGGGGRGTQAEVQINRDATVEVRCGTQDLGTGSRT
                     VIAIVAAEVLGLSPDKITVRIGDTRFPPSGGSGGSTTTASVSPAVFDACTNALAELQK
                     VSGIRDARGANWVAACKKIGMNPLVVSGKWREGLSSAGAGGVQFAEVEVDTETGFVNI
                     RKILVVQDCGLIVNKLTCESQINGGVIQGIGYGLYEQRVMDRATGVVLNPNFETYKLP
                     NGADVPEIEIVLLDMPERGVIGVGEPCHIPTAAAIANAVANAIGVRVSTIPITPDKIL
                     AALGKLPARKTADAATMEEAFALVRALPATVVEEFEPFIV"
     gene            complement(3840..4535)
                     /locus_tag="VE486_04060"
     CDS             complement(3840..4535)
                     /locus_tag="VE486_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZA38294.1"
                     /translation="MSKPSDVSDSSSGALQGGSVSRRNFLKALGTTAVASAASQARGV
                     AQELQKFNDEKIHGPGSVPVALKINGETKRFEVEPRVTLLDLLRNRTNLTGTKEVCDR
                     ATCGACTVLLDGTPVYSCMTLAIEAQRHEITTIEGLSKGGELTPVQKAFVNCDGLQCG
                     FCTPGFVMSVTALLQRNPKPTEEEVRKACAGNLCRCGTYPRVFAAALEAAGAKSASKA
                     EVIRLTQADVRLA"
     gene            complement(4589..5269)
                     /locus_tag="VE486_04065"
     CDS             complement(4589..5269)
                     /locus_tag="VE486_04065"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HZA38295.1"
                     /translation="MKIICVFLSLFISAELASAAPRPSGRVVELHACEVYTGGCTASA
                     QATLGGRSLLRLWSFENGAHSGVDLAGLQVAALQVADKNLALSDTQSSAAVVYLPAQA
                     TELQRQALVSWLKSTNPEIATARLVEKVASISYARDDSRISVRVGKEIALQTRAIGRC
                     EMGGCGEALWYQPRSKTGKFTVLVNENSSVEEPALSLIWKDHAVKSVFFARFGDDSKP
                     EFRLAAVE"
     gene            5529..5963
                     /locus_tag="VE486_04070"
     CDS             5529..5963
                     /locus_tag="VE486_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZA38296.1"
                     /translation="MKKDDSIQLSAVEDHDGIFTIRGGGGRRNWNGIHYKQGMSAKNV
                     GTTKLSANIATIPPGGVAYAHIHVGFEVILYILEGKVRHEFGPGLKQVIENEAGDFIY
                     IKPGVPHEVFNMSDTEPVVAFVARSSADEWDNIIPYNRNQAD"
     gene            5974..6501
                     /locus_tag="VE486_04075"
     CDS             5974..6501
                     /locus_tag="VE486_04075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZA38297.1"
                     /translation="MPKYLVISTSGNPSSNSRRMGRIAFAHLQKRKVTCDWMDIREID
                     LPLCDADKCYGMPGSRKLTTAVERADGVLVAAPVYNYDVAAATKNMIELTGSAWENKI
                     VGFLCAAGGHASYMSVMAYANSLMLDFRCVIIPRFAFATSDAFDGERISDKKITQRIK
                     QVADELVRFTRALRS"
     gene            6722..>8347
                     /locus_tag="VE486_04080"
     CDS             6722..>8347
                     /locus_tag="VE486_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38298.1"
                     /translation="MPPCGDFHRQDLGDAGLMNNEQDRRGTWVQRIWWHIRDLYHQLK
                     PCRFSFLVVLIACPVFLSVAQGTEILRTVGEGMAISGQWYWPRVCGFFAALILWAVCS
                     WYSARVLLYFDSPGAYRGPSSKFAGTHIPRILGVAPILIVACGFFVASRSYDAGTSVW
                     NWLIGFGIFSALLAVIVYLLFIARRKWLRSGPRLEVRRIRDLPGGTLVGVGIIALISL
                     ALFLAFTIRPVPIAQFMGMGTILLLAASSWVALGSFLVFLGLRWQFPVLTVVLLLACL
                     FSLWNDNHVIRQVPARAVKRAHLLQAFQTWYDGVEKNYGAGTMHPLYIVATEGGGIRA
                     AYWTAILLGEIQDRNPNFAPHLFAISGVSGGSLGATVFDALLAEPNRGSFKFKDKAHA
                     ILGRDFLSPTLAAMLYPDLAQRFIPFPIPYFDRGRALEMAWEKAWRDSMGNDRFSRSF
                     VDLWPPDSREWMPALFLNGTSVEKGNRIIASNVHLTNNFLDAEDAADKLAGYKLPATE
                     AACHIPLSTAAHMSARFTFVSPAGHFPDGSRIVD"
BASE COUNT         1695 a         2464 c         2287 g         1901 t
ORIGIN      
        1 atgcgtgtgg ctgttcagtt tccaaaggct gaagccgttg agtcggccat cgcatgcaaa
       61 tccgctcgcg attcgtgtcg cgccccgcag ctcctaatgg catttgtcag ttacatggaa
      121 cttcgggtgc gcgcggattg aatccgttgt aacgaaagcg tttagtgaag tcgaacattt
      181 tttcacagtg gaccagcctc catcccataa cctgacagaa aatttcgttc tcctggcaat
      241 caaatttaca taacactcaa acccaacgat cttttagaat tcaagttcac ctgttatgaa
      301 ggatgacaaa gacaacatcc aactcagcgc ggtggaagat cacgacggga tattgactat
      361 tcgcggcggc ggcggccgta gagattggaa tggcattcat tgcaatggag atgtaggggc
      421 gcgtcttcac gcgcagaacg tatcgaacat ttgccagtcc gactgggact caagaacttg
      481 aggaatccgt cggtcgtctc tcgcagctgc ctggttacgg agctttgtaa caagtgcgtt
      541 ccgcgctcga acatctggag aagcagacca ggttatcgtc cgcaccgatt tccgtcatcg
      601 tcttattgca ccaacagtgc gaaaatgcac cggggtcgaa tttgtccgaa aaggctgcct
      661 cctcctgatc cggcaaatac atttctttgt tccggaaata tctacacggg gtcggccggt
      721 gcatgtgact agctaatgag cttgaagagc gttcgtctaa taagcgcatg cgcgagcggc
      781 accttgtaag cgttgtgctc gaagggtgtc gctcctttta gcaataaatc ggcagcttgc
      841 gcggcgacgc gatcatccag cgatttatct tcgagaaatt tgtcggcctc gtccgagcga
      901 taaggaaccg gcgcgacctg gccgagcaca acgcgtgcgc cggacagctt cctgccgtcc
      961 acgcgggccg cggcggagca gcttaccagc gcccagtcga agtcgtgttt ttcgctcacc
     1021 tgcaaataag cacttcgcgt ttgccgcacc ggaacttcga cgcgcaggat taaatccgcc
     1081 ggagcgagtg aattatgcgc aagcggattc gaccatgcat tttcgtagag gctgggtatc
     1141 gaaagcgtga cgactttgcc ggagcgctgg accgcgacgg ttgcctcaag cgcagcaaga
     1201 gcgattgcca gattcgaggg cgttgggcta atgcacgggt ttccgctaaa gagcgaatgg
     1261 tacttgttct cgccctcgcg cgcgtaacag gtggagccac cttctttaag acaacgcacg
     1321 tcgcgatggc gataatacca gcaacgcgag tgctgcgcga gattaccgcc aagggtggcg
     1381 acgttgcgaa tctgagggga agcgacttcg gccgcggctt cggctaatcc ggggaatgtc
     1441 ttccgcattt ccgcgtgcgc ggcaagttcg gcgacagtga cgttcgcgcc gatgctccac
     1501 gattgcgggc ccgttttgat cgcgcgcgta tccggaagcg acttgatgtt gaccagccgt
     1561 ttcgtctcga acaggtaatc ctttagttcg ccaagaatgt cgatgccgcc ggcgatgtaa
     1621 cgtccgcttc cgccgattaa ttcggaggcg ctctgcggag tttgcgcggt gacgtattcg
     1681 aacggtttca tacgatgaat ggctcgaact cctcgaccac tgtcgcaggc aacgcgcgca
     1741 ccagcgcgaa cgcttcctcc atggtcgcag cgtcggcagt tttccgagcc ggtaatttcc
     1801 cgagcgccgc gagaattttg tcgggcgtga ttgggatagt gctgacgcgc acaccgattg
     1861 cgttcgcaac tgcatttgcg atcgctgccg ccgtcgggat atgacatggc tcgcccactc
     1921 cgatgacgcc gcgctctggc atgtccaaca gcacaatctc gatctcaggc acgtccgcgc
     1981 cgttcggcag cttgtaagtt tcgaagttcg gattcaggac aacaccggtg gcgcgatcca
     2041 tcacgcgctg ctcgtaaaga ccataaccga tcccttgaat gacgccgccg ttgatctggc
     2101 tctcgcacgt gagcttgttg acgataagac cgcaatcttg aacgacgaga atcttgcgga
     2161 tgttgacgaa accggtctcg gtatcgactt ccacttcggc aaactgcacg cctcccgcgc
     2221 cggcggatga aagaccttcg cgccatttgc ccgacacaac gagcggattc atgccgatct
     2281 ttttgcacgc ggccacccag ttggcgccgc gggcatcgcg aatgccactg actttttgca
     2341 gttcggcgag cgcattggtg caggcatcga agaccgcagg agagacggag gcagtggtcg
     2401 tgctgccacc gctgccacct gagggcggga accgcgtatc tccaatgcgc actgtgatct
     2461 tgtcgggact aaggccaaga acttcggccg ccacgatggc aatgacggtt cgcgatcctg
     2521 tgccgagatc ttgggtcccg cagcgcactt caacagtggc atcgcggtta atctgcacct
     2581 cggcttgagt tcctcgtccg ccgccgcccc aagttgcccc ggcgcagccc acgccagtct
     2641 taacaatccc ggaactcgag cccggcttcc gatatttttt cttccattca aatttttccc
     2701 gcccgatctc gtattctttt cgccggatct cgaacggatc atttttgatc cggatatcca
     2761 ccggatcgat gccgagcttt accgccaggt cgtccatcat tgactccatg ccgaacgagg
     2821 cggttggatg acccggtgca cggaacgctc gcgaggagcc tgcattgata gccacgctcg
     2881 cctgcttcac ccgcgtgttc gggattttgt aaatgtaggg cgcaggaaac gtcgcgccgc
     2941 tgccccctcc cgccgttgct ccgccggcag caaaaccggg acagccaaat gagtccagct
     3001 cgtatgccaa cagggttcca tctgcggccg cgccgagctt caccttctgg aaactcgatg
     3061 ggcggttgcc gacggcaagg gcttcgtcga aacgggtgag catcagtttc accggagcgc
     3121 gagctgcctc ggagagtcgc gcggcgagac ctccttccaa gccgggcccg aacttcgatc
     3181 cgaagccgcc acccatgtag tcgcaaatca cacgcacttt gctttgcgac acatcgaggt
     3241 tgtcggccat cccgtcgcgc acgctaaaga tgccctgcgt ggaagcccag acggtgattt
     3301 cctcgggcgt gaaggaaacg gtgttgccgt gcgtttcgag cgcgtgatgc aactgaacct
     3361 gtgtctcgag cgtgttctcg atgatcgcgg ccgatttgga aaagccttcg gctacgttgc
     3421 cgtcctcacg aacctgcgct tcgccgacgt tcgcgccatc ctgaaaaatt ttcggagcat
     3481 cgggcttcat cgcgtcgagt tccttcacca caaacggcag cggagttgcc tggacttcaa
     3541 tcagcgtcaa ggcgtccaga cacgcttcct tactggtgcc ggcgatggcc gccagttcct
     3601 cgccataata gcgcacttgg cgttcgcctt cgcgcgccag caccgcggct ttgattccgg
     3661 gtgctgctct cgccttctcc aagttgatcg acgtaatttt tgctgcggcc cacttcgatc
     3721 gcagaatcgc tccccagagc cagccggtcg gctgaacgtc gtaggcgtac tttgccgcac
     3781 cgctcacttt cgccgggccg tcaatacgcg gcgcgggttt gccgagatat ttgatttctt
     3841 taggccagtc ggacatcggc ttgagtaagg cgaatgactt cagctttgct cgcgctcttc
     3901 gcgcccgcgg cttccaacgc cgccgcaaaa acacgcggat acgtgccgca acggcagaga
     3961 ttgccggcgc aggctttgcg cacctcttct tcggtcggct tcgggttgcg ttgcagcaga
     4021 gcggtaacgc tcatgacaaa gcccggcgtg cagaaaccac attgtagccc atcgcagttg
     4081 acgaatgcct tttgcaccgg cgtcagctca ccgcctttcg agagaccctc gatagtggtg
     4141 atctcgtgcc gctgcgcttc gattgccagc gtcatgcaag aataaaccgg agtgccatcc
     4201 aataagaccg tgcacgcgcc gcaggtcgcg cgatcgcaga cctctttggt tccagtcagg
     4261 ttcgtgcgat tgcgcagcaa atcgagcagc gtcacgcgcg gctcgacctc gaaccttttc
     4321 gtttcgccgt tgattttcag cgccaccgga acggagccgg gaccgtggat tttctcgtcg
     4381 ttaaatttct gcagctcctg agcaacaccg cgcgcctgcg aagcagctga tgctaccgcg
     4441 gtggtgccga gcgcttttaa gaaattgcgc cgggaaacag agccgccctg caaggcgccg
     4501 gaagacgagt ctgatacgtc gctcggtttg ctcatcgtat caccaagttg ctgctgttta
     4561 tggcaacgct ggcgcagggc gtaaagacct attcgacagc ggcaagacga aattccggct
     4621 ttgagtcatc accgaaccga gcgaagaata cgctcttcac agcgtgatcc ttccagatca
     4681 gcgagagcgc tggttcttcg accgaagaat tttcattcac cagaaccgtg aacttccccg
     4741 ttttgctgcg cggctgatac caaagcgctt ctccgcagcc gcccatctcg caacgcccga
     4801 ttgcgcgagt ttgcagcgcg atctccttcc ccactcgaac agagatgcgc gaatcatccc
     4861 gcgcatagct gatcgaagcc actttctcca ccagccgcgc agtcgcgatc tcaggattcg
     4921 ttgacttgag ccaacttacc agcgcctggc gctgaagctc ggtcgcctgc gccggtaggt
     4981 aaacgaccgc tgccgagctt tgcgtatcag aaagcgcgag attcttgtcc gcaacctgaa
     5041 gcgccgcgac ttgcaaacca gctaggtcca caccgctgtg tgcgccgttt tcaaagctcc
     5101 agagtcgcag cagggaacgt ccgcccagcg tcgcttgggc cgaggccgtg caaccgccgg
     5161 tataaacttc acaagcatgc aattcgacga ctcgtcccga aggacgtggt gctgcgctcg
     5221 ccaattctgc gctgatgaac aaactcaaaa agacacagat gattttcatg ttcttcctct
     5281 tagttggatt ctatgtgatg cgaaaaccag tgatgaatca ctagcagtag cgtcgaccaa
     5341 acaggagtgt cgtcaatcga aaacaagtcg tcccgctatg caaataagaa ttttccctca
     5401 cgaatagccg ccccggattg aacctcaatc ccgcaccatt cttcaccagc agccgcttac
     5461 tgaatctgga actcaggaac tagagaaaaa acttcctgct ttcctgattt ccttagagat
     5521 tgcctgcaat gaagaaggac gactccattc aactcagcgc ggtagaagat cacgacggga
     5581 tattcaccat ccgcggcggc ggcggccgcc ggaattggaa cggcattcat tataaacagg
     5641 ggatgtcggc caaaaatgtg ggtacgacca aattatcagc caacatcgcc acgatacctc
     5701 cgggtggcgt cgcctacgcg catattcatg tcggtttcga agtgatcctt tatattctgg
     5761 aagggaaagt gcgccacgaa ttcggacccg ggctcaagca agtgattgag aacgaagccg
     5821 gcgatttcat ttacatcaaa cccggcgtgc ctcacgaagt cttcaatatg agcgacacgg
     5881 aacctgtcgt cgcctttgtc gcccgctcct ctgctgacga gtgggacaac attattcctt
     5941 acaatcgcaa tcaggctgac tgattcggtg ccgatgccaa agtatctcgt cattagcaca
     6001 agcggtaatc ccagcagcaa tagccgccgg atgggacgga tcgcgttcgc gcatctgcaa
     6061 aagcgaaagg taacgtgcga ttggatggac atccgtgaaa tcgatctgcc actctgcgac
     6121 gcagacaaat gttatgggat gcccggctca agaaaattga cgacggctgt tgaacgcgcc
     6181 gatggggtcc tggttgcggc gccggtatat aattatgatg tcgcagccgc gaccaagaac
     6241 atgatcgagc tcaccggaag cgcgtgggaa aacaaaatcg tcgggtttct ttgcgccgcc
     6301 ggaggccacg ccagttacat gtcggtcatg gcctatgcca acagcctcat gctcgatttt
     6361 cgctgcgtca ttattccgcg ctttgcgttt gccacgtccg atgctttcga cggcgagcgt
     6421 atcagcgaca aaaaaatcac ccagcgaatc aaacaagtcg ctgacgaact cgttcgcttc
     6481 accagggcgc ttcgaagtta aaagcgtcgc cggttctctc tcgttgctgt catcgtcgtt
     6541 tgatgtcagc cccgtacgca aatcgcgccc acgggggcgg gcgcgatcgt ctaacaatgg
     6601 agatgagaaa tgaagattag gtctgggctt ccttctcgcc ctttatgcat tccgctttga
     6661 acttcgcgaa ctgctccttc gctcagcgat tagcttgacc aaagaattcg cttttggctg
     6721 aatgccgcca tgcggcgact tccatcggca ggatttgggc gatgccggct taatgaataa
     6781 cgaacaggat cgacgcggca cctgggttca acgcatctgg tggcacattc gcgatcttta
     6841 ccaccaactc aaaccttgtc gtttcagctt tctggtcgtt ctcattgcct gtcccgtgtt
     6901 tcttagcgtc gctcaaggca ccgaaatcct gcgcactgtc ggtgagggca tggccatcag
     6961 cggacagtgg tattggccgc gggtttgcgg attcttcgcc gccctgattc tctgggctgt
     7021 ctgtagttgg tacagcgcgc gggtcctgct ttattttgat tcgcccggcg cgtaccgtgg
     7081 gccatcctca aaattcgccg ggactcatat tccgcgcatc ctgggcgtgg cgccgatatt
     7141 gatcgttgcc tgcggatttt tcgttgcttc gcgatcttat gacgcgggaa cgtctgtttg
     7201 gaactggctg atcggtttcg gcattttctc cgcgcttctc gctgttatcg tgtatctgct
     7261 gttcattgcc cgccgaaaat ggctgcgttc cgggccccga ctcgaggtga gacgcatccg
     7321 ggatctcccg ggaggcactc ttgtaggtgt gggcattatc gctcttattt cgctggcgct
     7381 ctttctcgcg tttacgatca ggcccgtgcc cattgcccaa ttcatgggga tgggcaccat
     7441 cctgctgttg gctgcttctt cctgggtcgc cctcggaagt ttcctggttt tccttggtct
     7501 gcgctggcaa tttcccgtcc taaccgtcgt ccttctgctc gcctgccttt ttagtctttg
     7561 gaatgacaac catgtcattc gccaagtccc tgcccgggcg gtgaaacgcg cacacctgtt
     7621 gcaggcattc caaacttggt acgatggagt ggaaaaaaat tacggcgcgg gtacgatgca
     7681 tccgctttac atcgttgcca cggaaggggg cgggattcgg gcggcctact ggacggcgat
     7741 tcttctcggt gaaattcagg ataggaaccc gaatttcgca ccgcatctat tcgccatcag
     7801 cggcgtttcg ggcggaagcc tgggggccac tgtcttcgac gcgctactcg cggaacccaa
     7861 ccgcggttca tttaagttca aggacaaggc gcacgctatc cttggccggg attttctttc
     7921 gcccacgctg gccgcgatgc tctatcccga tctggcccag cgtttcattc cctttccgat
     7981 tccctatttc gatcgcggac gcgcgctcga gatggcctgg gaaaaagcgt ggcgcgattc
     8041 catggggaat gatcgttttt cgcgttcctt tgtcgatctc tggccaccag attcgcgcga
     8101 atggatgccg gcacttttcc tcaacggcac ctccgtcgaa aaaggaaatc gcatcattgc
     8161 gagcaatgtt catctcacga acaactttct tgatgccgag gacgccgccg acaagctggc
     8221 cggttacaag ctgcccgcga ccgaagcagc ctgtcacatt cccctcagca ctgcggcaca
     8281 catgagcgcg cgcttcactt tcgttagccc ggctggacat ttcccagatg gctcgcgcat
     8341 cgtcgac
//
LOCUS       DASQUQ010000143         4126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_119243, whole genome shotgun sequence.
ACCESSION   DASQUQ010000143 DASQUQ010000000
VERSION     DASQUQ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119243"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1051
                     /locus_tag="VE486_04085"
     CDS             98..1051
                     /locus_tag="VE486_04085"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZA38299.1"
                     /translation="MRILVTGGAGFIGSHLVEKLLATGHEVAILDDFNDFYDPQIKHA
                     NIAGFAKDAAIHHIDLRDSASVQNVFQRAKFETIAHLAARAGVRPSIQHPQLYCDTNV
                     SGTLHLLDAARVTGVERFIFASSSSVYGSSKIVPFSEDQHLTQTLSPYAATKIAAEFF
                     CSTYAHLYPMRVVSLRYFTVYGPRQRPDLAIHQFTRRIYAGEPIEQFGDGTTRRDYTF
                     IDDVIQGTMAALKYDGPRFDIFNLGESDTIQLKDLISAIENALGKKAKINRLPEQPGD
                     MPLTCADISKARKLLGYNPTTRFSDGLPRFIEWFLQSARGK"
     gene            1054..2061
                     /locus_tag="VE486_04090"
     CDS             1054..2061
                     /locus_tag="VE486_04090"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZA38300.1"
                     /translation="MKDLPFDQRACFIAGQAKSGTTLLVALLDNHPELLVLPEETAYF
                     PTVLTKYGPRGRRAQFDYITKESLSNVLFGGPCKWGKRDYSYFPTARFLQTFEQMAFD
                     PLNAERDLLVILLEAYAAILGRSLGSITRWVEKTPANRKYLPAIHTRFPHARVLVTMR
                     DPRAIFAAQIALEKTRQLGTFSTYYCISHWRDVAKVVLRARDCSDTTLFDVPYEKLIL
                     EPAKWMKQICAFLEIEYDPAIVLTPTKAGKFWEGNSATETAFFRISTEPLTRWQSDLS
                     EEEIGWVEWHCRDLMPEFGYEPRLGERSLRHFMKPTRYERPRQYLKSRFYSLRDDWIR
                     R"
     gene            2578..3078
                     /locus_tag="VE486_04095"
     CDS             2578..3078
                     /locus_tag="VE486_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZA38301.1"
                     /translation="MNSYSSGRRSRRSRGGSRRRGGPERFRGQQEETKAKKQTFWQRI
                     AAFFGDGTGTKKTATPARNGTQPSRPSRKPETVEVTSPRLYVGNLSFDATESDLFELF
                     NGVGHVQNAEVVSYRHNQRSKGFAFVQMQTIEEAKRAVQELHDKEFLGRRLVVSGAKS
                     SEQMRS"
     gene            3176..>4126
                     /gene="miaA"
                     /locus_tag="VE486_04100"
     CDS             3176..>4126
                     /gene="miaA"
                     /locus_tag="VE486_04100"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZA38302.1"
                     /translation="MSLARAFFIVGPTAAGKSELAADVAREMGAEVVSADAFQIYSGL
                     NLLTAKPNATTLATAPHHLIGTMPLDQEMNAEKFRRAASRAIDEIQSRGKEAIIVGGS
                     GLYVKALTHGLAPLPKADPKLRAKLHALGTDALRSQLAHLDPETARKIDSKNRRRLVR
                     ALEICLLTGKPVSVQHTQRAVAGGVEPGPPRSPPAATKGSTITATFRGVFVFRDRDEL
                     YERINRRAEAMFEEGVIEEVRAAGAVSSTASQMIGLREIRELLAGKMSIAQCVAAIQQ
                     ATRRYAKRQLTWFRRQTNFEPLNLSLLSHNEAVNWILLRAQ"
BASE COUNT          951 a         1137 c         1117 g          921 t
ORIGIN      
        1 cgtgtcagca gcaataaaag cgatgccgaa ggacgcatcg aggtgcctaa tccgaaatct
       61 gtgtcgatct atgtctatct gtggctaatc tagctgcatg cgaattctcg tgaccggtgg
      121 cgctgggttc attggatcgc atcttgtcga gaagctgctc gcgaccggac atgaagtcgc
      181 cattctcgat gactttaatg atttttacga tccgcaaatt aagcacgcaa acattgccgg
      241 tttcgccaag gacgcagcca ttcaccatat cgatctccgg gacagcgcct cagtgcaaaa
      301 tgtgtttcag cgcgcgaaat ttgaaacgat cgcccatctg gctgcacgcg cgggcgttcg
      361 cccctcgatt cagcacccgc aactttattg cgatacaaac gtgagcggca cgttacatct
      421 tctcgatgca gcgcgtgtca ccggggtgga gcgattcatt ttcgcgtcca gctcgtccgt
      481 ttacggcagt tcgaagatcg tcccattttc tgaagatcaa catcttactc agacgctgag
      541 tccctatgcc gcgacaaaaa tagctgctga atttttctgc tcgacttatg cgcaccttta
      601 tccaatgcgt gtggtttcgt tacgatattt tacggtttac ggtccgcggc aacgacccga
      661 tctggcgatc catcaattca cccggcgcat ttacgccggt gagccaatcg agcaattcgg
      721 tgatggcacg acgcgccgcg attacacttt catcgacgat gtgattcaag gaacaatggc
      781 cgcgctcaaa tatgatggtc cccgcttcga catcttcaat ctcggcgaga gcgacaccat
      841 tcagcttaaa gatttgatca gtgcgatcga gaatgcgctt ggtaaaaaag caaaaatcaa
      901 tcggcttccg gagcaaccgg gcgacatgcc gctgacttgc gccgacattt caaaggcaag
      961 aaaattactc ggttacaatc caactactcg attcagtgac gggctgccgc gttttatcga
     1021 atggtttctt caatcggctc gcgggaaatg aagatgaaag acctgccatt tgatcaacgt
     1081 gcctgtttta ttgcgggcca ggcgaaatcc ggcaccacgc ttttggtggc gttgctcgat
     1141 aatcatcctg agctgctcgt gttgccggag gaaaccgcct atttccctac tgtcctcaca
     1201 aaatatgggc cgcgcggccg ccgcgcgcag tttgattaca tcacaaagga gtcgctctcc
     1261 aacgtgctgt ttggtgggcc gtgcaaatgg ggcaagcgcg actactccta ttttccgaca
     1321 gcgcgcttcc tccagacttt tgagcagatg gcgttcgatc cgttgaacgc tgaacgagat
     1381 ttgctcgtaa tcctgctgga agcgtatgcc gcgatcctcg gtcgctcact cggttctatc
     1441 acgcgctggg tcgaaaagac gccggccaac cgcaagtacc tgcctgcgat ccacacgcgg
     1501 tttccgcatg ccagggttct tgtaacgatg cgtgatccgc gcgccatttt cgccgcgcag
     1561 atcgccttgg aaaaaacccg gcaactggga acgttctcca cctactattg catcagtcac
     1621 tggcgcgacg tggcgaaagt ggttcttcgc gccagggatt gcagcgacac gacactgttc
     1681 gatgtgccat acgaaaagct tattcttgag ccggcgaaat ggatgaagca aatctgcgcg
     1741 tttctcgaga tagaatacga tccggccatt gtgctgacgc cgacaaaagc cgggaaattc
     1801 tgggaaggaa attccgctac ggaaaccgcg tttttccgga tcagcaccga accgttgacg
     1861 cgctggcaaa gtgacctgtc agaggaggaa attggatggg tggagtggca ttgccgcgat
     1921 ctcatgccgg aattcggtta cgagcccagg cttggtgaac gctcgttgcg ccacttcatg
     1981 aaaccaactc ggtacgagcg acccaggcaa tatctcaaat cgcgattcta ctcgctgcgc
     2041 gacgattgga ttcgccgcta gaggtcatcc ggctctgttg aaaacttaac ccgctcgttg
     2101 tcattccgag cggagtcgag gaatctctca ttattaagat agtaatcccg cagtggcggg
     2161 agactccgct cgataagaca gcaatggaac tggtttccaa cagagccagg tcatcccgag
     2221 tcgcggagat cgtgggcgct tttttcgtcg cacttgcaat cgcgcacgca gcgagccata
     2281 atcaacgcga gatgtccgga atcttcttcg atcttccgag cctccgtttt ctacgacgat
     2341 ccagattttt tgagaccagg atgcgaacct ttgcatcttc actgcttcgt tggttgagcg
     2401 cgctggcttg cggttcgaga caagtccatt tccggcttaa caaggtggag tttccggcgg
     2461 cgtcgcagag tgcttgcaaa ctgtttgaat ggaccctggg tgattcgcgc gccgcggact
     2521 gtgcatcaat cgatgcgctc cgcccattta acaattgggt caaacttaag aatcaacatg
     2581 aactcttatt catctgggag acgctcgcgc cgttcgcggg gcggatcccg acgcagaggc
     2641 ggccccgaac gctttcgggg tcagcaggaa gaaacgaaag cgaagaaaca gacattctgg
     2701 cagcggatcg ccgcattttt cggagacggc acaggcacaa agaaaaccgc aacgcccgcg
     2761 cgcaatggta cacagccttc ccggccgtca cgaaaacctg aaacggtcga agtcacctcg
     2821 ccgcgtcttt acgttggcaa cttgtctttc gacgcgacgg agagcgatct cttcgaattg
     2881 ttcaacggcg tcgggcacgt gcaaaacgcg gaggtcgtaa gttatcggca caaccagcgt
     2941 tcgaagggtt ttgcgtttgt tcaaatgcaa accatcgagg aagcgaagcg ggccgttcag
     3001 gaattacacg acaaagaatt cctcggccgt agactggtcg tgagcggcgc aaaatccagc
     3061 gagcaaatgc gaagctaaca agcttgtcgc ttcaatcggg agacgtggcg cagctgagcg
     3121 cgcgtctccc gattttgttt tccggaacat acgtcatatg ggtcgtatag gtcccatgag
     3181 cttggcgcgc gcatttttca tcgtcggccc caccgcggcc ggtaaatccg agctggcagc
     3241 cgatgtggca cgtgaaatgg gcgccgaagt tgtcagcgct gatgcgtttc aaatttatag
     3301 cggcctgaat cttctgaccg cgaagccgaa tgcaacaaca ctcgcgacgg ctccccatca
     3361 tttgattggc acaatgccgc tcgaccagga gatgaacgcg gaaaaatttc gccgcgcggc
     3421 atcgcgcgcc attgatgaaa ttcagtcgcg cggaaaagag gcgatcattg ttggtggaag
     3481 cgggctttat gtgaaagcac tcacccatgg attggcgcct ctgccaaaag ccgatccgaa
     3541 gctgcgagcg aaattgcatg cattgggcac agatgcgttg cgctcgcaac ttgcccatct
     3601 cgacccggaa accgcgcgca agatcgattc aaaaaatcgt cgtcgcctcg ttcgcgctct
     3661 cgaaatatgc ctgctcaccg gaaaaccggt ttcagtccaa cacacgcaac gggctgtagc
     3721 cgggggcgtt gaacccggcc cgccgcggtc accgcccgca gctacaaagg gatcaacgat
     3781 cacggctaca tttcgcggcg tgttcgtgtt ccgcgaccgc gatgagcttt acgaacgaat
     3841 caatcgcaga gccgaagcaa tgttcgaaga gggcgtgatc gaagaagtgc gggctgctgg
     3901 agcggtcagt tcgactgcct cccagatgat cggtttgcga gagatccggg aacttctcgc
     3961 aggcaaaatg tccatcgcac aatgcgtcgc cgcgattcaa caagcaaccc ggcgttatgc
     4021 caaaaggcaa ttgacctggt tccggcggca aactaatttt gagccgctga acttatcgct
     4081 tctcagtcac aacgaagctg tgaattggat tctgctgcga gcgcag
//
LOCUS       DASQUQ010000144         6167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_677297, whole genome shotgun sequence.
ACCESSION   DASQUQ010000144 DASQUQ010000000
VERSION     DASQUQ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677297"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VE486_04105"
     CDS             complement(<1..481)
                     /locus_tag="VE486_04105"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38303.1"
                     /translation="MTLTVPTRADIPESDKWDLTQLFAEAGKWQEDFARLQRAYPKLE
                     EWKGRVGESAQTLADVLEFEKSLEVKIERVYHYASLQLAEDGTDNEYLTRVGQVQNLL
                     TKIGEALAFVVPEIQAIDDETFAKFMLDPALADWRIKLHKIRRMKPHVLSVPEERLLA
                     "
     gene            complement(586..1128)
                     /locus_tag="VE486_04110"
     CDS             complement(586..1128)
                     /locus_tag="VE486_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HZA38304.1"
                     /translation="MQSTPVSGGAVVGDVMLIGQAPGPREPVLQRPFAHTAGKTMFRW
                     FEQFCGLNEATVRSKIYFAAVCRCFPGKTSAGGDRVPAPDEIRNCSSWMDDEIRILKP
                     RLVIPVGRLAIAQFIDCGKLERVIGRKFHIERTGHRFNLIPLPHPSGASPWHKISPGK
                     ELLERALKLIARHPAIIDLR"
     gene            complement(1309..1554)
                     /locus_tag="VE486_04115"
     CDS             complement(1309..1554)
                     /locus_tag="VE486_04115"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HZA38305.1"
                     /translation="MPPRNSRNLLSMRELGKFVVIIGIIMTLVGLVMWSGFAPKWLGR
                     LPGDIRIEREHSAFYFPIVTCIILSIVLSLLLSIFRR"
     gene            1569..2525
                     /locus_tag="VE486_04120"
     CDS             1569..2525
                     /locus_tag="VE486_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZA38306.1"
                     /translation="MAVQFRDYYETLGVPKTATEDEIRTAFRKLARKHHPDVAKDKKA
                     AEEKFKEINEAYEVLSDSEKRKKYDQLGADWNRPGGFQPPPQWGAQPPEGGFYQWGGD
                     GGGGVQFEFGGTGFSDFFEAFFGGGRGRSAFGGFGGRQATAERGADVEADIMVTLEEA
                     LHGSTRTVSLRRAGSNKVENYQVKIPRGVHEGQRIRLAGQGEAGVRGGKSGDLFLRVR
                     LAKHPDFSVDGSDLIHEVKIEPWRAVLGSELLVPTLEGHVRLKIPPGTQGGQRFRLRE
                     RGLPSSSGTRGNLYVDVQINVPKKVTEREREIWRELAKLHGG"
     gene            2642..3073
                     /gene="rpiB"
                     /locus_tag="VE486_04125"
     CDS             2642..3073
                     /gene="rpiB"
                     /locus_tag="VE486_04125"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZA38307.1"
                     /translation="MKISLGTDHAGFRYKEKVKELLGTLGHEVKDFGTFNEEPVDYPL
                     FIRPAAEAVARGECERGIVFGGSGNGEAMVANKVHGVRCALCWNEESTRLSRQHNDAN
                     VLSIGERLIPEDLALKIVRIWLETPFEGGRHERRVAQLNAM"
     gene            complement(3138..4013)
                     /locus_tag="VE486_04130"
     CDS             complement(3138..4013)
                     /locus_tag="VE486_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HZA38308.1"
                     /translation="MDTKDRQGERRGFGGPVSRFLRLIRFSHTIFALPFALGALVVAA
                     NGLPSLRTFLLVVVCMVLARTAAMLFNRLVDWSLDQRNPRTASRHLLVSKSAMLILLA
                     LSSAGFILAAEAINRLTFLLAPVALALIFFYSLTKRFTSTTHFFLGLALAIAPVGAWI
                     AQTGHIDLAPLVLGAGVICWVAGFDLIYATQDYDFDRREGIQSLVVKLGIARSLRLAQ
                     LLHLAMFAAFVGFGVFAQLGLFYYAGMPFVAAALFYEHKTEKLDLAGINRAFFRSNAF
                     VSAVFLVAVCVDRLT"
     gene            complement(4026..4679)
                     /locus_tag="VE486_04135"
     CDS             complement(4026..4679)
                     /locus_tag="VE486_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZA38309.1"
                     /translation="MSSLDSTPMFSSVRPAPDFDRIASDTAIWHAYDPGVKAELYSTC
                     LLTSAGACLIDPIPLQSETLEEVVRPGRVAGVIVTSSNHHRNAVQFAEQFSAPIFACA
                     TAFPDNQPRELRRVADGDEICDGLRVIGIDGAAAGEVVLHYAPNDGTLIVGDALINFE
                     PYGFTFLPGKYCSNEKEMRRSLRKLLDYKTERILFAHGTPILSRASDQLRRLLDSDR"
     gene            complement(4892..>6167)
                     /locus_tag="VE486_04140"
     CDS             complement(4892..>6167)
                     /locus_tag="VE486_04140"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HZA38310.1"
                     /translation="YSLTRNTTGSQNTANGYSALYFNTEGDDNTAAGFAALNRNTTGV
                     SNVAVGNQALFRNTSGAGNCAVGQFALEFNTVGDTSNAFGFAALLNNDIPSGIAGNNA
                     FGFVALASNVTGPDNAAFGDSALASNIISAANCAFGNFALFNNDSGGAGLANGNSAFG
                     TEALTSNVDGETNNAFGQFALVGNVDGIWNNAFGFNALGLNVSGSFNTAIGDEAGLNI
                     NGDGNVCIGEGVVGEAGVNDRTYIRNVNTTVQAGAGFDTVTVRLADGRMGHAVSSRRY
                     KDDIKPMDKASEALFALKPVMFRYKKQVDPAQGLDYGLIAEDVAEVAPELAVHNQKGE
                     IENVRYQAIYAMMLNEFIKEHSTVEQLKATVAKQEATIAQQQKEFHAAIVQQRKDFEA
                     VVAQLKDQAAQIQKVSAQLEVNKPAPQAVANR"
BASE COUNT         1435 a         1716 c         1770 g         1246 t
ORIGIN      
        1 atgcgagaag ccgttcctca ggcacggaaa gaacatgcgg tttcattcga cgaattttgt
       61 gcaacttgat tcgccaatcg gccaacgctg gatcgagcat aaactttgcg aaagtttcat
      121 cgtcgatcgc ctggatttcc ggaactacga acgccaacgc ctcgccgatc tttgtaagca
      181 agttctgaac ctgaccgacc cgagtgagat actcattatc cgtgccgtcc tccgccagtt
      241 gcagggacgc gtagtgatag acccgctcga ttttcacctc gagcgacttc tcgaactcaa
      301 gcacgtcggc caacgtttgc gccgattccc ctactctgcc cttccactcc tcaagtttcg
      361 gatacgcccg ctgaagccgg gcaaaatcct cctgccattt cccggcctcg gcgaaaagct
      421 gcgtcagatc ccatttgtcg gattccggga tgtcagcgcg cgttggaacg gtcaaagtca
      481 ttcaggaacg tcgaacgtcc aacacccatc gtccaatatc gaagcgtgat tgttggatgt
      541 tcgatgttcg gcgttcgtag gggcggcgcg accggcggtc gcaccctacc gaagatcgat
      601 aattgccgga tggcgcgcga ttaatttgag cgcgcgttcg agtaattctt ttccgggtga
      661 aatcttatgc caaggcgagg cgccggatgg atgcggcaag ggaataagat tgaagcggtg
      721 acctgtgcgc tcgatgtgaa atttgcgacc gatcactctc tccagtttcc cgcaatcgat
      781 aaactgcgcg atggcaagac ggccgaccgg aattacgaga cgcggcttta gaattcggat
      841 ctcgtcatcc atccaggaag agcaattgcg aatttcgtcg ggcgccggga cacgatcgcc
      901 gcccgccgaa gttttaccgg gaaaacatcg gcagactgct gcgaaataaa tcttcgagcg
      961 aacggtagcc tcattcagcc cacagaattg ctcaaaccag cggaacatgg ttttacctgc
     1021 agtgtgtgcg aagggccgtt gtaaaaccgg ctcgcgcggg ccgggcgcct ggccaatcaa
     1081 catcacatcg cccacgaccg ccccgccgga gacgggcgtc gattgcatgc gcgggcaccg
     1141 ccggcattgc aggagatgag cgacgtggcc gtcgagtcga gtttgaatgt cttttgtagc
     1201 cacggccctg tgggccgttc gcgcgtgccg tggcaacacc ccaaggcaga cgggccacag
     1261 gcccgtggct acagtagcgc ctgcgataca atgatcattt gaaaatggtc agcgccgaaa
     1321 gatcgacaat aacaaactca acacaatgct gagaatgatg caggtgacga tgggaaaata
     1381 aaatgcggaa tgctcgcgct cgatccggat gtcgcccggc aatcgcccca gccatttcgg
     1441 cgcgaagccg ctccacatta ccaggccgac aagcgtcatg atgatgccaa tgatgaccac
     1501 gaattttccg agctcgcgca tcgagagaag gttgcgcgaa ttgcgcggag gcacaaggtt
     1561 gagagccgat ggcggtgcag tttagagatt attacgaaac gctcggtgtc ccgaaaacgg
     1621 ccaccgagga cgaaattcgc accgcttttc gcaagctggc ccggaaacat catcccgatg
     1681 tcgccaaaga caaaaaggcg gcggaagaga aattcaaaga gatcaatgaa gcctacgagg
     1741 tgttaagcga ctcggagaaa cgaaagaaat atgatcaact aggggccgat tggaacaggc
     1801 cgggcgggtt tcaaccgcca ccgcaatggg gcgcacagcc acccgaaggc ggattttacc
     1861 agtggggcgg cgacggtggc ggcggggtcc agttcgaatt tggcggaacc gggttcagcg
     1921 atttcttcga agcgtttttc ggcggcggtc gcggccggtc tgctttcggc ggattcgggg
     1981 gacgccaggc gacggccgaa cgtggggccg acgtggaagc cgacatcatg gtcaccttgg
     2041 aagaagcgct gcatggatcg accagaacgg tttcactccg ccgggccggt tcgaacaagg
     2101 ttgaaaacta ccaagtcaag attccgcgcg gcgtccatga ggggcagcgc atccgcctgg
     2161 ctggtcaggg cgaagcaggt gtgcgcggag gaaaaagcgg cgacctcttt ttacgcgtgc
     2221 gtttggcgaa acatcccgat ttctcggtcg atggcagcga tctgattcat gaggtcaaga
     2281 ttgagccgtg gcgcgcagtg ctcggcagcg aattgctcgt gccgactctg gaaggacatg
     2341 tccgactgaa aattcccccc ggcacccagg gcggtcaacg ctttcggctg cgggaacgcg
     2401 ggcttccgag cagttcggga acacgtggaa atctttatgt ggatgttcag atcaacgttc
     2461 cgaagaaggt aacggaacgc gagcgagaga tttggcgcga actggcgaag ctgcacggcg
     2521 gatgatggat tgtcatgtcg agcgaagtct cccgccgctg cgagattact attgtctttt
     2581 ctggcgaggc gactgggtca acaattagag attcctcgac ttcgctcgga atgacatatc
     2641 catgaaaatc tcgctcggca cggatcacgc cggttttcgg tacaaagaaa aggtcaaaga
     2701 attgctcggc acgctcgggc acgaagtgaa agatttcggc acctttaatg aagaaccagt
     2761 cgattacccg cttttcattc gtccagcggc ggaagcggta gcgcgcggcg agtgcgagcg
     2821 cgggatcgtg tttggcggat cgggcaacgg cgaagctatg gtggccaata aagtgcacgg
     2881 ggtgcgttgc gcgctttgct ggaatgaaga atcgacccgg ctttctcggc aacacaatga
     2941 cgccaatgtc ctttccatcg gtgaacgcct gatccccgaa gatctggcgc tgaagattgt
     3001 ccggatctgg ctcgaaacgc cgttcgaagg cggccggcat gaaaggcgcg tcgcacaact
     3061 caacgcgatg tagtaatcgc cttcctggta gggtcggtgc gctgcgccga ccggacgccg
     3121 cgacgcggta ttcctaccta agtcaatcgg tccacgcaca ctgcgaccaa aaaaacagcg
     3181 ctcacaaatg cgttgcttcg aaaaaatgcg cgattgattc cagccagatc gagtttctcc
     3241 gttttgtgct cgtaaaaaag ggccgccgcc acgaaaggca ttccggcata ataaaataat
     3301 ccaagctgcg cgaagacgcc gaacccgacg aacgcagcga acattgcgag atgcagtaat
     3361 tgcgccagac gaaggctgcg cgcgatccca agcttgacga caagcgattg aataccttcg
     3421 cgccggtcga aatcgtaatc ctgcgtggcg taaatgagat cgaaccccgc cacccaacaa
     3481 atgacccccg ccccgagcac cagcggtgca agatcgatat gtcccgtctg cgcgatccag
     3541 gcgccgaccg gagcaatggc gagcgccagt ccaagaaaga aatgcgttgt gctggtgaag
     3601 cgcttcgtga gtgaatagaa aaaaatcagc gccagagcga ccggcgcgag caggaaggtc
     3661 aggcgattga tcgcctccgc cgccaaaatg aagccggcgg aactcaacgc gagcaaaatg
     3721 agcatcgccg atttggaaac gagcaagtga cgcgaggcgg tgcgcgggtt gcgttgatcg
     3781 agcgaccaat caaccaatcg attgaaaagc atcgctgcgg tccgggccaa taccatgcag
     3841 acgacaacca acaaaaacgt gcgcaaggac ggaagaccat tggcggccac gaccaacgct
     3901 ccgagagcaa atggaagcgc aaaaatagtg tgggaaaaac gaatcaaccg aagaaatcgc
     3961 gacaccggtc cgccaaatcc cctccgctca ccttgtcgat ctttagtgtc cacagcaaat
     4021 cagattcagc gatcactatc cagcagtctc cgcaactgat cgctcgctct agacaaaatc
     4081 ggggtgccgt gcgcgaaaag gattcgctcc gttttataat ccagaagttt ccgtaatgac
     4141 cgtctcattt ctttttcatt cgagcagtat tttccaggga gaaatgtgaa gccgtacggt
     4201 tcaaaattga tcagcgcgtc gccaacaatc agcgtcccgt cattgggcgc gtaatgcaga
     4261 acgacttcgc cagctgctgc gccatcgatg ccaatcacgc gcaaaccgtc gcaaatctcg
     4321 tcgccatcgg cgactctgcg caattcgcgc ggctggttat ccggaaatgc cgtagcacac
     4381 gcgaagatcg gagccgagaa ctgctctgcg aactgaacag cgttacggtg atgattagaa
     4441 ctcgtcacaa taactcccgc aacacggccg ggccggacaa cttcttcgag cgtttcgctc
     4501 tgcagaggaa tcggatcgat aaggcaagcg ccggcggaag tcaggaggca agtcgaatag
     4561 agctccgcct taacgcccgg atcataagcg tgccaaatcg cggtgtcaga agcgattcgg
     4621 tcaaaatcgg gcgccggtct caccgatgag aacatcggcg ttgaatccag actgctcaag
     4681 atcgcgcagg tgcagggtag tgtaggattt ggcacccaaa cgatctgggg agcacaggct
     4741 gccagcctgt agttgccggc agccctgccg gcaacattct tttgcaaacg taattctgag
     4801 ataacgccgg aatgccccgt ctcggcaagc tgccgagacc tgcaggctgg cagcctgtgc
     4861 tccccagaaa ggaccgcggc tagacaacct gttacctatt ggcgactgct tgcggcgcgg
     4921 gtttgttcac ttcaagttgt gcgctcactt tctggatctg cgctgcctga tccttgagtt
     4981 gcgcgacgac tgcctcaaag tctttccgct gctgcacgat ggccgcatga aactccttct
     5041 gctgctgtgc gatcgttgct tcctgctttg ccactgtggc ctttagctgc tccaccgtgc
     5101 tgtgttcttt gataaactcg ttgagcatca tggcgtagat cgcttggtat cgcacattct
     5161 cgatttcgcc cttttgattg tggacggcca actcgggggc cacttcggcc acatcctcgg
     5221 cgatcaagcc atagtcgagg ccttgcgcgg gatcgacctg tttcttgtag cggaacatga
     5281 ccggtttgag cgcgaacagc gcttcgctgg ctttgtccat cggcttgata tcgtccttgt
     5341 aacggcgcga tgaaacggca tgacccattc tgccgtcggc cagcctgaca gtcacggtat
     5401 caaacccagc gccagcctgc accgtggtat taacattgcg gatataggtt ctgtcattga
     5461 cgccggcctc accgacgact ccctcgccaa tgcacacgtt gccatcgccg ttgatattca
     5521 accccgcttc atcgccgatg gcggtgttga agctgccgct tacgttgaga ccgagcgcat
     5581 taaacccgaa tgcgttgttc cagattccgt cgacgttgcc gacaagcgcg aactgaccga
     5641 atgcgttatt ggtctcgccg tcaacgttgc tggtgagcgc ttccgtgcca aaggcactgt
     5701 tgccatttgc cagcccggca ccaccggaat cgttgttgaa gagcgcgaaa ttgccaaagg
     5761 cacagttggc cgccgaaatt atgttcgaag cgagagctga atcgccgaaa gcggcgttgt
     5821 ctgggccagt tacgttgctg gccagcgcta caaaaccgaa cgcgttgttc ccggctatgc
     5881 cacttggtat gtcgttgttc aaaagcgcgg caaaaccgaa tgcgttactg gtatcgccaa
     5941 cggtgttaaa ctccagtgcg aactggccaa cggcacagtt gcccgcgcca ctggtattgc
     6001 gaaagagcgc ttgattaccg acggccacgt tactgacacc ggtggtgttg cgattgagcg
     6061 cagcaaaacc agcggccgtg ttatcgtcac cttcggtgtt gaaatagagc gcagaataac
     6121 cgttggctgt gttttgcgag ccggtggtgt tgcgcgtcag agaataa
//
LOCUS       DASQUQ010000145         5132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_393520, whole genome shotgun sequence.
ACCESSION   DASQUQ010000145 DASQUQ010000000
VERSION     DASQUQ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393520"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /gene="sufB"
                     /locus_tag="VE486_04145"
     CDS             complement(<1..1343)
                     /gene="sufB"
                     /locus_tag="VE486_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZA38311.1"
                     /translation="MKTETETKAVDINRNIGDFFYPEAHVRDAGTGLSERTVQYISDV
                     KEDPDWVREYRLRGLKTFLEKPLPTHWASKDLEAIDFDKIRYYLAPGTEAKRTWEEVP
                     EDIKRTFERLGIPEQERKFLAGVEAQFDSEAVYSNIKKAVGEQGVIFVGSTEGLKNYP
                     EIFRKWFGKVIPIADNKFSALNSAVFSGGSFIYVPPGVKVKHPLQAYFRINAENFGQF
                     ERTLIIADEGAEVTYMEGCTAPKFNTPTLHSAVVELVALKGAKIQYITVQNWASNVFN
                     LVTKRGLAQENAEVKWIDCNIGSRLTMKYPSVIMKGRKARGEVLSIALANDGQHQDTG
                     AKMIHAADETTSNIISKSISIGKGRATYRGLVHVPKHLKHCKNNTECDALLINATSRT
                     DTYPAITVRGTGNAVQHEASVSQVSADQIFYMQQRGLTEAQAMSLSVNGFVNDLVR"
     gene            complement(1353..2120)
                     /gene="sufC"
                     /locus_tag="VE486_04150"
     CDS             complement(1353..2120)
                     /gene="sufC"
                     /locus_tag="VE486_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZA38312.1"
                     /translation="MKELNVEKLHVSIGEQEIVRGLSLRVPRGEVHALMGPNGSGKST
                     LAKVLAGHPDYRVTSGKVLMDGEDLLELEPDDRARRGLFMAFQYPSEIPGVTIANFLR
                     AAMQSRLPEGEELEATDYYAKLYEKMELLGMDRSFTSRAVNEGFSGGEKKRAEILQLA
                     MLEPKYAVLDETDSGLDIDALKTVAHGVNSLRGPDLGVLLITHYQRLLNYIIPDYVHV
                     MVAGRIVRSGRKELALELEERGYDWAREGAAEPAFTR"
     gene            complement(2215..2288)
                     /locus_tag="VE486_04155"
     tRNA            complement(2215..2288)
                     /locus_tag="VE486_04155"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2254..2256),aa:Gln,seq:ttg)
     gene            2425..4179
                     /locus_tag="VE486_04160"
     CDS             2425..4179
                     /locus_tag="VE486_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38313.1"
                     /translation="MYRLTAGSNTVMAQTTFLGHYRISVDDNGTPHELGRDGAVIAYE
                     AVDERSGESVAITLVPVESIDPAVREQFEEQARAMQRLRHVNIAKVFDFGRERENYVY
                     VCERLPGETLAAWVARHGPMPADATLRVAEQMVSVLSSASFHKLPYPPIDPSGILVVP
                     GQTPEGGWPLVKLTNFGLAGLKSDPENSPRDEASFHTGAGELAPKDAALHERAPSAAQ
                     SATAESRTFTGEHETTDFRSEIYSLGATMYFLLTGVALSAEALRRPPKLSGFPKPLRN
                     LLSQMLHPNPDRRPKDPVALAEMIRECLLKIERRQLLARRLGIPLAAVIPRKSRTTPT
                     PLAQVLRGILVFAAIVLVAGVVGAFLLPADLNPFRHRTAAKQVIGVPVGVPETSPVAP
                     AEAVTTTPVMANQPVTNAAASPAAKETSSPSLEEAQSSQGDFVAAATPASPPETSSNT
                     PAAGPEESAQTQDTPSESGPSGSAPQPATATESTSEGKQKSAPSASKRARVTQDSTYG
                     RRRAPMRSARARVVGITSDGRLIFRLSSGRTMIVTPGSENEDEIAPSRRRRAFTPQAR
                     DEIFTPSQPFPPDYFPDD"
     gene            4372..>5132
                     /locus_tag="VE486_04165"
     CDS             4372..>5132
                     /locus_tag="VE486_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38314.1"
                     /translation="MRRRLSLPLAILSLLAISTAGAHAQAFASSTLSLASPEQETKKD
                     IGPAITKEPVAVTTKQSAPRTTKKQARASAPAPAPARSFAIEPVRAIAKPYQSEATRP
                     VARYSFKTPQGKKESVPVITQYYPKQIVYPLAKVDRQIDRKLMQAATIAQERAHAHSR
                     SRCWHYVKDALLASGAIDSRPKTAYAKDAAQELVSNYGFKKLSVTDPFAAPVGSVLVY
                     GARRAAGHVEIRTRDGFVSDFRSKTPSPRPLLGVFA"
BASE COUNT         1065 a         1502 c         1420 g         1145 t
ORIGIN      
        1 cggacgagat cattgacgaa accgttgacg ctcaagctca tcgcttgcgc ctcggtcaga
       61 ccgcgctgtt gcatgtagaa aatctgatcg gcgctcacct gactcacgct ggcttcgtgc
      121 tgaacggcgt tgccggtacc gcgtacagtg atggccggat aggtatctgt ccgcgaggtc
      181 gcgttgataa gcagcgcgtc acactccgta ttgttcttgc aatgcttgag gtgcttcggc
      241 acgtggacta ggccgcgata agtggcccgg cctttgccga tcgagatcga tttggaaata
      301 atgttgctcg tcgtttcatc ggctgcgtga atcatcttcg caccggtgtc ctggtgctgg
      361 ccgtcgttgg cgagggcgat ggaaagcact tcgccgcgcg ctttgcggcc tttcataatc
      421 acgctgggat atttcatggt gaggcgcgag ccgatgttgc aatcgatcca ttttacttcg
      481 gcgttctcct gggcgaggcc gcgttttgtg acaaggttga agacattcga cgcccagttc
      541 tgcactgtga tgtactgaat tttggcaccc ttgagagcga ccaattccac cacggcgctg
      601 tgcaacgtag gcgtgttgaa tttcggtgcg gtgcaacctt ccatgtaagt cacttctgcg
      661 ccctcatccg caatgatcag agtgcgctcg aactggccga agttttcggc gttgatacga
      721 aagtaagcct gaagcgggtg tttcactttc acgccgggcg ggacgtaaat gaacgagccg
      781 ccgctgaaca cggcagaatt cagtgcgctg aatttgttgt cggcgatcgg gataactttg
      841 ccgaaccatt tccggaaaat ctccggataa ttcttcaggc cctctgtcga gccaacgaaa
      901 atgacgcctt gttcgccgac ggctttcttg atattcgaat agaccgcttc gctgtcgaac
      961 tgcgcttcca caccggcgag gaattttcgt tcctgttcag gaatgccgag acgctcgaac
     1021 gtccgcttga tgtcctcggg cacttcctcc caagtccgtt tcgcttctgt gccgggcgca
     1081 agatagtagc ggattttatc gaaatcgatt gcttcgaggt ccttgctcgc ccaatgcgtg
     1141 gggagcggtt tttccaggaa cgtcttcaac ccacgcagac gatactcgcg cacccaatcc
     1201 gggtcctctt tgacgtccga gatgtactgg actgttcgct cgctcaggcc agtgcctgca
     1261 tcgcgcacgt gtgcctcggg ataaaagaaa tctccaatgt ttcgattaat atcgacggct
     1321 tttgtttcgg tttcggtttt cattgtcgat gtttacctcg taaacgccgg ttcagcggct
     1381 ccctctcgtg cccaatcgta acctctttct tcgagctcaa gcgcgagctc cttgcgaccg
     1441 cttctaacga ttcgccccgc taccatcaca tggacgtagt cgggaatgat atagttcaag
     1501 aggcgctgat agtgcgtgat taataatacg ccaagatcgg gaccgcgcaa cgagtttacc
     1561 ccgtgtgcaa cggtcttgag cgcgtcgata tcgaggccac tgtcggtctc atcgagaacg
     1621 gcatatttgg gctcaagcat cgccagttgc aggatttcgg cgcgcttttt ttcgcccccg
     1681 gaaaaacctt cgttgaccgc ccgtgaagtg aacgaacgat ccattccgag taactccatt
     1741 ttttcgtaaa gttttgcgta atagtcggtt gcctcgagct cctcgccctc cggcagacga
     1801 gattgcatgg cggctcgcag gaagtttgcg atggtcacgc ccggaatctc gcttgggtac
     1861 tggaaagcca tgaaaagccc gcgccgcgcc cgatcatccg gctcaagctc gagaagatcc
     1921 tcgccatcca tcaatacctt gccagaggta acccggtaat cggggtgccc cgccaggacc
     1981 ttggccagag tgcttttccc cgagccgttc ggacccatga gcgcatgcac ctcgccgcgc
     2041 ggaacacgga gacttagtcc gcggacgatc tcctgttcgc cgatggacac atgcagtttt
     2101 tctacgttca attccttcat aagagcgaat cacaaatata agctgctccc gcggatttgc
     2161 gaacagtcat ttggagcgct aacggcacct tttgtagcgc cgagcatccg gatctggcta
     2221 ccccgcttgg attcgaacca agaataacgc ctccaaaggg cgctgtgtta ccgttacacc
     2281 acggggtatg cggatttgcg attttagatc ggcgattttc gattcgagcg caatcgccaa
     2341 aatattcggg cggtgggcgc gtgattagtt tgcataagca actcacggcc aaaatcgagg
     2401 ttgaatggta gcggggcggg ctgattgtac cgtctcaccg cgggcagcaa tacggttatg
     2461 gcgcaaacca catttctcgg gcattaccgg atctcggtag acgacaacgg gacgccacat
     2521 gaactcggcc gagatggcgc tgtgattgct tacgaagccg tggacgaacg gtccggtgaa
     2581 tcggttgcca taacgttggt ccctgtagaa agcatcgatc cggctgtccg ggagcaattc
     2641 gaagagcagg cgcgcgctat gcaaaggctg cgtcacgtca atatcgccaa ggtttttgat
     2701 tttggacgcg aacgtgaaaa ttacgtttac gtctgcgagc gactgccagg cgaaacactt
     2761 gctgcgtggg ttgcccggca cggcccgatg ccggctgacg ccacgttgcg cgtggcggaa
     2821 caaatggtga gcgtgctctc gtccgccagt tttcacaaac ttccgtatcc gccaattgat
     2881 ccatcgggca tccttgtcgt tccgggacag actcccgaag ggggttggcc actagtgaaa
     2941 ttaaccaact tcggcttggc cggattgaag tctgatcccg aaaactctcc gcgggacgaa
     3001 gcctcgttcc atactggggc gggcgaactc gcaccaaagg atgcagcgtt acatgaacga
     3061 gcgccttccg cagcgcagtc tgcgaccgct gaatcccgga cgtttacggg agagcatgaa
     3121 actacggatt tccgttcgga gatttattcg ctcggcgcga caatgtattt tcttctcacc
     3181 ggcgttgccc tttcggctga ggcgctccgg cggcctccga agttgtcggg gtttccaaaa
     3241 ccgttgcgaa acttgctttc ccagatgttg catcccaatc ctgatcgacg tccaaaggat
     3301 ccggtcgcgc tcgcggaaat gatccgcgaa tgtctgctaa aaattgagcg tcggcagctc
     3361 ctcgcgcgga gactgggaat tccgctggcg gcggtaattc cgcgaaaatc gaggacaacc
     3421 ccaacgccgc tcgcgcaagt gctgcgcgga atacttgtct tcgcagccat tgtattagtg
     3481 gcgggggtgg tcggtgcctt tttattgcct gccgatctga atccgttccg gcatcggacc
     3541 gcggccaaac aggtgatcgg cgtccccgtt ggtgtgcccg aaacgtcgcc ggttgcgcca
     3601 gcagaagcgg taacgaccac gccggttatg gccaatcagc cggtaacgaa cgcggcggca
     3661 tctccagctg caaaagaaac ttcctctccg agtcttgagg aggcacagag ttcacagggc
     3721 gactttgtcg cagctgcaac tcctgcgagc ccgccagaga cgagctcaaa tactccagca
     3781 gctgggccgg aagaatctgc tcaaacccag gataccccat cagagtcggg ccccagcggg
     3841 tctgcaccgc agcccgccac tgccactgag tcaacttcgg agggcaaaca gaaaagcgcc
     3901 ccttcggcgt caaagcgggc gcgtgttacg caggattcaa cctatggccg gcgacgagct
     3961 cccatgaggt cggcgcgcgc tcgggttgtt ggcatcactt ccgatggaag attgattttc
     4021 cgcttgtcct ccggccgcac catgatcgtc acgcccggtt ccgagaatga ggacgaaatc
     4081 gctccaagtc ggcgccgccg cgctttcacg ccgcaggcgc gcgacgaaat cttcactcca
     4141 tcgcagccgt ttcctccgga ttattttcct gacgactagc cacctgacca gcagacctcg
     4201 agacggggac gtggtgagac gatagtcgcc gcttttcgcg ccagcttgtt catctcgtgc
     4261 gtatactttt cggcagaggc ggcttctcct ggccaacgcc tgccaaatcc cctaaaaatt
     4321 ttttaaaaaa aagcttgccc aacgtcgcaa ttcccgccat tactcgcctc cgtgcgacga
     4381 cgactctcct tgccattggc aattttatcg cttctcgcca tctccaccgc aggcgcacac
     4441 gcgcaggcat tcgcctcttc aacattgagt cttgcgagtc ccgagcagga gacgaaaaag
     4501 gatattgggc cggcgatcac gaaggagccg gtcgcagtaa ccacgaaaca atccgcgcca
     4561 cggaccacga aaaaacaggc tcgcgcgagt gcgccggcgc cggcgccggc gcgatcgttt
     4621 gcaatagagc cggtccgggc aatcgcgaaa ccataccaaa gtgaagccac tcggcctgtt
     4681 gcccgttaca gcttcaagac cccgcaggga aaaaaggaat ccgttcccgt catcacgcag
     4741 tattatccga agcagatcgt ttacccgctt gctaaagtcg atcggcagat cgatcgcaag
     4801 ctgatgcaag cggcgacgat cgcccaggaa cgcgcgcatg cgcattcgcg ctcgagatgc
     4861 tggcattatg taaaagacgc attgctcgca tccggtgcta tcgattcacg cccgaagacc
     4921 gcatacgcga aagacgccgc ccaggagctc gtcagtaatt acggattcaa aaaattatcc
     4981 gtcaccgatc cattcgccgc gccggtcggc tcggtgctcg tgtatggcgc caggcgcgcc
     5041 gccggtcacg tcgagatccg caccagggac ggtttcgtca gtgacttccg ttcaaaaacg
     5101 ccgtcgcctc gtcctttgct cggcgttttc gc
//
LOCUS       DASQUQ010000146         8207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_758254, whole genome shotgun sequence.
ACCESSION   DASQUQ010000146 DASQUQ010000000
VERSION     DASQUQ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758254"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VE486_04170"
     CDS             <1..1360
                     /locus_tag="VE486_04170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA38315.1"
                     /translation="EQLPKVTVQLPIFNEVYVAERLLRSVSELDYPQHLLQIQILDDS
                     TDETREIISSCADKLRYRGFNVQCLHRVERTGFKAGALAAGLEAAEGEFLCILDADFV
                     PQPDLLKRTIHFFTDPKIGMIQTRWGHLNRGYSLLTRMQAIFLDGHLLLEQTARSRSG
                     RFFNFNGTAGLWRRECIEQGGGWQYDTLCEDLDLSYRAQLAGWKFIYLSDVVTPAELP
                     VDMNGFKSQQHRWTKGSIQTCKKLLPTIWRSKLPFPIKLEATGHLMSNFAYLLLALLC
                     VLLHPSAGGLQGGWLRTLVIDVPIFFAASVSVAVFYVCAQRELYPRTWKRQILFLPCL
                     LGLGVGLSLNNARAVLEALFNHQSDFTRTPKYGIERKSQPWRTCKYRLAKPMLPLAEL
                     AFAIYFTYFVCFAIWHGQFLSVPFLLMFQGGFLYVCLSSFANRWPKMSPGNSRASAAI
                     PA"
     gene            1392..2090
                     /locus_tag="VE486_04175"
     CDS             1392..2090
                     /locus_tag="VE486_04175"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZA38316.1"
                     /translation="MFRPLALIFGLVSLAPALFATQLDPSHHLIISVRDQKLMLLQNG
                     TKVATYPVSTSKFGLGDFSGRMTTPLGYLAVAKKIGDRAPVGAVFHNRRFTGEILAPN
                     APGRDPVITRILWLRGLEGQNAHAFRRCIYIHGTPEEKTIGRPASYGCIRMKSSDVAA
                     LYNQVPLGAIVQIVPDHLPKVPKAPPAPSSTAFMARDEGTRSQDQPGVTRSTDKATQL
                     LNKRASIVQNSPRA"
     gene            2151..2399
                     /gene="rpsT"
                     /locus_tag="VE486_04180"
     CDS             2151..2399
                     /gene="rpsT"
                     /locus_tag="VE486_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZA38317.1"
                     /translation="MANTRSAAKRARQTMKRSLRNHSVVTRLRKIRRQVRSAAEPSAD
                     QIRAAISAIDKAAKRGIIHRNAAMRRKARLNKALAAAK"
     gene            2522..3118
                     /locus_tag="VE486_04185"
     CDS             2522..3118
                     /locus_tag="VE486_04185"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HZA38318.1"
                     /translation="MIPALLLIFSAVAYRVTTGLLIHSGASWLSNFAPLAAIALCSAA
                     YLPKKYKFSVPLITLFISDAVINFRYGAPLLDPQILVRYVALALVGCIGVLLQNRASL
                     KTLFPASIVGSTIFYAITNAFSWLSDPGYVKNLGGLIQALTVGLPQYSATPSWMFFRN
                     SLVSDLLFTLLFVVCMNLGRSTEKSRARTARFRPAAAG"
     gene            3115..4200
                     /gene="pdhA"
                     /locus_tag="VE486_04190"
     CDS             3115..4200
                     /gene="pdhA"
                     /locus_tag="VE486_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HZA38319.1"
                     /translation="MTAMQPGEQRDEVEMLALMLLIRRFEERASQQYQAQKIGGFCHL
                     YIGQEAVVTGAIAAVRDDDYVITAYRDHAHALARGTSADACMAELFGKETGCSRGLGG
                     SMHFFNRKNHMYGGHAIVAAHVPLAVGMAFATNYLDEDRVTLCFFGDGAINQGAFHEA
                     LNLAALYKLPIIFICENNLFAMGTSVKRSTSLKQIVDRAEGYDIPGEIVDGMNFREVR
                     DKIAEIVDSIRKEPHPAFLEVRTYRYRGHSMSDPASYRTKVELEKYRLDDPITRLRAQ
                     LTREGKLTNEQFDLLDKRAKETVLAAVKFAEKSPQPPLDRLYDYTYADGAVEVGVSPP
                     PEHREGESPRIEKKSAADTATAKKENG"
     gene            4197..5180
                     /locus_tag="VE486_04195"
     CDS             4197..5180
                     /locus_tag="VE486_04195"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF008854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HZA38320.1"
                     /translation="MREITYRQALNEALAEELERDPNVFLMGEEVAEYQGAYKVSQGL
                     LQRFGPRRIIDTPISENGFAGLGVGAAMVGLRPIIEFMTFSFSLVAFDQVVNNAPNMF
                     TMSGGQFNIPITFRGPNGPAHQLGATHSHATENFYASVPGLKVCTPATPRDAKGLLKT
                     AVRDNNPVLVLEAELLYSSKGMVEPPDEELLIPLGKAEVKREGSDVSIICYAQTVPLA
                     LKVASQLEEEDISAEIVDLRSIKPLDEKAIFDCVAKTHRVVIVEQDHPFCGVGAEVCY
                     RIQKNIFDELDAPIIRVSQEDVPMPYNERLEKAVLPNADKLIAAVKKVCYA"
     gene            5219..6634
                     /locus_tag="VE486_04200"
     CDS             5219..6634
                     /locus_tag="VE486_04200"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HZA38321.1"
                     /translation="MPKLSDTMTEGTLVAWKKKKGDNVSAGEVLAEIETDKATMEWES
                     PEDGTLSEIYVEEGGKVNVGDKIAFISGEGEEAPEEKPEKKDEAKKEGEEKPVEAEAE
                     EKEEAEIEKEEEKAKPKKEQAKKEPEKKEEKPKEEEKPKEPKPEEKEEKKAAPRKAEE
                     KAEAEKPAPAAEKPEEARVKASPVARRIAAELGVDLASVKGTGPEGRVTETDVRAAAK
                     SKVAAPQKAPSAAKAAAPSIKAGESARIQLSGMRKIIAQRLVESLGPVPHFYLNIDID
                     AGPLMEAREELKSAGEGADAAKITVNDFVLKAAVQAAVKVPRVNASFDGDAIVQYADV
                     DLGIAVAIEDGLLTPVIRAAQNKSLREISELGKDLAHRARNKRMKPEEFQGGTFTVSN
                     LGGMGIDSFSAVINPPQGFILAIGKITKVPVIDDGEQIVVGHRMSITMSCDHRVIDGA
                     LGAEYLKELRHLLENPALLLV"
     gene            complement(6799..7302)
                     /locus_tag="VE486_04205"
     CDS             complement(6799..7302)
                     /locus_tag="VE486_04205"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HZA38322.1"
                     /translation="MKKARKTKEKSRRRRKTRSAPKNLKENVPYRKPALRSEAFAKAL
                     ADAKSSANDPESLQALFNEAAKKAAAGPREAFKGNWPYLQAMLRFIQAYSSGTYSPRD
                     VSDDALLWMIAALNYFVDPFDLIPDNVPFLGFVDDATVVEFAVSRTKDTLDDFMIWET
                     RGGTISG"
     gene            complement(7510..>8207)
                     /locus_tag="VE486_04210"
     CDS             complement(7510..>8207)
                     /locus_tag="VE486_04210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZA38323.1"
                     /translation="HLVPDWLHLSEAVRTGAPPVAVNQEATGPEFFSVLVENIVPMSY
                     PAAAALGRVLNLAKTRKPMRVLDVAAGSGIWGIALAQQSPLVRVTAQDWAEMIPVTKR
                     ITQKFGVADRFTYIRGDVLEADFGNDYDIATLGHILHTEGEERSRKLLQKVFGALKPG
                     GTIAIGEWLVEDDRAGPLNGLIFAVNMLVHSERGDTFSFNEIKNWLEDSGFKKVRKLQ
                     APGPSPLILATKA"
BASE COUNT         1914 a         2232 c         2303 g         1758 t
ORIGIN      
        1 tgaacaattg ccgaaggtga ccgtgcagtt accgatcttt aatgaggttt acgtggccga
       61 gcgtttgctt cgctcagtca gtgaattgga ctatccgcag caccttctcc agatccagat
      121 actggatgat tcgaccgacg agacgcgcga gatcatctca tcgtgcgcag acaaattgcg
      181 ctatcgcggc tttaatgtgc aatgtctcca tcgggttgag cgcaccggtt tcaaggctgg
      241 cgcgctggcg gctggcctgg aagccgcaga aggcgagttc ctttgtattc ttgatgccga
      301 ttttgtcccc cagccagatc ttctaaagcg cacaattcat ttctttaccg atccgaaaat
      361 aggaatgatc cagaccaggt gggggcactt aaatcgtggc tattcgctgc ttactcgcat
      421 gcaggcgatt tttctggatg gacatctttt gctggagcaa accgcccgca gccgcagcgg
      481 ccgttttttt aatttcaacg gaactgccgg tctgtggcgg cgtgagtgca tcgagcaagg
      541 cggaggctgg cagtatgata cgctatgcga agacctcgat cttagctatc gggctcagct
      601 cgcgggctgg aaattcatct atctttccga cgtggtaact cctgcggagt tgccggtgga
      661 tatgaacggc ttcaaatctc agcaacatcg ctggacaaag ggttcgattc agacgtgcaa
      721 gaaattgctg cctacgatat ggcgcagcaa actcccgttt ccgatcaaac tggaggccac
      781 cggtcatttg atgtccaact tcgcctacct gctacttgcc ttattgtgcg tcctcctgca
      841 tccctccgca ggcgggcttc aaggcggatg gctgcggacg ctggtcattg atgttccgat
      901 cttcttcgcc gcgtcggtct cggttgcggt tttttatgtt tgcgctcaac gcgagctata
      961 tccgcgcacg tggaagagac aaatattgtt tctgccgtgc ttactcggcc tcggcgttgg
     1021 cctttcactc aataacgcgc gcgctgtgct agaagcgctc ttcaatcacc aatcggattt
     1081 tacacgcact cccaagtatg gaattgaacg caaatcacag ccttggcgaa catgcaaata
     1141 tcggctggcg aaaccgatgc tgccattggc ggagctggcc ttcgcgattt atttcactta
     1201 ctttgtttgc ttcgcaattt ggcatggaca gttcctgtcg gtgccctttt tgctcatgtt
     1261 ccagggcggt ttcctttatg tttgtctctc ttcattcgcc aaccgctggc caaaaatgag
     1321 ccctggcaac tcacgtgcga gcgccgcaat tcccgcatga caaacgccga ttatctgcta
     1381 aaaaggtgcg catgtttcgt ccccttgctc taattttcgg tttagtttcg cttgctcccg
     1441 cgcttttcgc cacacaactc gacccttccc atcacctgat aataagcgtc cgcgatcaga
     1501 agctgatgct gctgcagaac ggcacaaaag tggcaaccta ccccgtttcc acatccaaat
     1561 tcggactggg cgatttttcg ggacgaatga caactcctct tggttacctt gcggtcgcaa
     1621 aaaaaattgg agaccgcgca ccagtcggcg ccgttttcca caaccggcga ttcaccggag
     1681 aaattctggc gccaaacgct ccgggtcgcg atccggtgat tacacgcatc ctttggctgc
     1741 gcggcctgga aggtcaaaat gcccacgcct ttcgtcgctg catttacatt catggcacac
     1801 cggaagagaa gacgatcggc cgcccagcca gctacggctg catcaggatg aaatcgagcg
     1861 acgtcgctgc gctatacaat caagtcccgc tcggagcgat tgtgcaaatc gttccagacc
     1921 atctgcccaa agtgccaaag gcgccgccgg cgccctcgag tactgcgttt atggccaggg
     1981 atgaaggaac taggagccag gatcagcccg gcgtgacgcg ctcgacagat aaagcgacgc
     2041 agctgctgaa caaacgggcg tcaatcgtcc aaaatagccc gcgggcataa acgcccattt
     2101 gctccgaatc ggcatcggcg ctgtccacct caaagttttt cgatcaaaca atggccaaca
     2161 cgagatcggc agccaaacgg gcccgccaga caatgaagcg atcacttcgt aatcacagcg
     2221 ttgtgacgcg tctgcggaag attcgaagac aggtgcgttc cgcggcagaa ccaagtgctg
     2281 atcaaatccg cgcggccatc tcggcgatcg ataaagctgc aaagcgcggc atcattcatc
     2341 gaaacgccgc catgcgtcgc aaagcgcggc tcaacaaagc gctcgcagcc gccaaatagt
     2401 ggagtagtga agtaacggag tgatggagca atgaactcgc tctgaaatca atactccaac
     2461 actccattga agcgcggcaa atgccctttg catatctcgc gggacagata gattcttccg
     2521 aatgattccc gctcttctcc tcattttttc cgcggtcgct tatcgcgtta cgacggggct
     2581 cttgattcat tccggcgcca gctggctttc caacttcgct ccgctcgcag cgattgcgct
     2641 ttgcagcgca gcgtaccttc cgaaaaagta caaattctcg gtgccgctca ttacgctctt
     2701 tatttccgat gcagtaatta actttcgtta cggcgcgcca ctgctcgacc cgcagattct
     2761 tgttcgttat gtcgcgctgg ctctggtcgg atgcatcgga gttctcctgc agaatcgggc
     2821 ttcactcaaa acccttttcc cagcttcgat tgttggatcg acaatttttt acgcgatcac
     2881 gaacgcgttt tcctggttga gcgatccggg ttacgtgaag aatttgggag gacttatcca
     2941 ggccctgacg gtgggactgc ctcagtacag cgcgacgccg agctggatgt ttttccgtaa
     3001 ctcactcgtc agcgatcttc tgtttactct gctctttgtt gtttgcatga atttggggcg
     3061 aagcacggag aaatcgcgcg cccggaccgc gcggttccgt cccgcagctg cgggatgacc
     3121 gccatgcagc cgggggagca gcgcgacgaa gtcgagatgc tcgcgcttat gttgttgatc
     3181 cggcgctttg aggaacgcgc cagtcaacag tatcaagcgc agaaaatagg cggcttttgc
     3241 catctttaca ttggccagga agccgtcgtg acaggcgcta tcgcggcggt tcgcgacgac
     3301 gactacgtca ttaccgctta tcgcgaccat gctcacgcgc tcgctcgcgg gacaagcgcg
     3361 gacgcatgta tggcagagct tttcggaaag gagaccggct gctcgcgggg cctgggtggc
     3421 tcgatgcatt tcttcaatcg gaaaaaccac atgtacggcg gtcacgctat tgtggctgct
     3481 cacgttccat tggcggttgg aatggctttt gccaccaatt acctggacga ggatcgcgtc
     3541 acgctttgct tcttcggtga cggcgcaatc aatcagggcg ctttccacga agctctaaat
     3601 ttggccgcgc tttataaatt gccgatcatc ttcatttgcg aaaacaacct cttcgccatg
     3661 ggtacgtcgg taaagagatc gacatcgctc aagcaaatcg ttgatcgggc ggagggctac
     3721 gacattccgg gagaaattgt cgatgggatg aactttcgcg aagtgcgcga taagatcgcc
     3781 gagattgtgg attcgattcg gaaagagccg catccggcgt tcctcgaagt ccgcacttat
     3841 cgctatcgcg gtcactcaat gtccgatccg gcaagctacc ggaccaaggt ggaactggag
     3901 aaatatcgcc tggacgaccc aatcacgcgt ttgcgtgcgc agctcacgcg cgaggggaaa
     3961 ttgaccaacg aacaattcga tctgctcgac aaacgcgcga aggaaacggt tctggcggcc
     4021 gtgaagtttg ctgagaaaag cccgcagccg ccgctcgata ggctttacga ttacacttat
     4081 gccgacggag ctgtggaggt aggcgtctcg cctccgccgg aacatcgaga aggcgagtcg
     4141 cctcgaatcg agaagaaatc cgcagccgac acggctaccg ccaaaaaaga gaacggatga
     4201 gggaaatcac ttatcgtcag gcactcaacg aagcgctcgc ggaagagctt gagcgcgatc
     4261 ccaacgtttt tctcatgggc gaagaggttg ccgagtacca gggcgcttac aaagtaagcc
     4321 agggtttgct tcagcgtttt gggccgcggc ggatcatcga tactccaatc agtgagaacg
     4381 gatttgccgg tctgggagtt ggggcggcaa tggtcggatt gcggccgatt atcgagttca
     4441 tgacgttcag cttttcgctc gtcgcatttg atcaggtcgt caataacgct ccaaacatgt
     4501 tcacgatgtc gggcgggcag ttcaatatcc ccatcacgtt tcggggaccg aatggaccgg
     4561 cgcatcaact cggcgccacc cattcgcatg cgacggaaaa cttttacgct agcgttccgg
     4621 gtctgaaagt ctgcacgccg gcgacaccgc gtgacgcaaa aggcctgctg aaaacagccg
     4681 tgcgcgacaa taatcccgtg ctggttctgg aagcagaact gctttacagc tcgaaaggaa
     4741 tggtcgagcc gccggatgaa gagctgctca ttcccctggg caaagcggag gtgaaacgcg
     4801 aaggcagcga tgtctcgatt atctgctacg cgcagacagt tccgctggcg ctcaaagttg
     4861 cgtcccagct cgaagaggaa gacatctctg cggaaattgt cgacctgcgc tccatcaagc
     4921 cgctcgatga gaaagcgatt ttcgattgcg tcgcaaaaac gcatcgcgtg gtgatcgtcg
     4981 aacaagatca tccgttttgc ggcgtaggcg cagaggtctg ttaccggatc caaaaaaaca
     5041 tttttgatga gctcgacgcg cccatcatcc gcgtatcaca agaagatgtt ccaatgccct
     5101 ataacgaacg cctcgagaag gcagttttgc caaatgcgga caaactgatc gcggccgtga
     5161 agaaagtctg ttacgcgtaa tctctgaccc ctgattctcc gccatgcctg agattcaaat
     5221 gcctaaactg agcgacacga tgactgaggg aactctggtg gcgtggaaga aaaaaaaggg
     5281 cgacaacgtt tcggccgggg aagtgcttgc cgagatcgag acggacaagg cgacgatgga
     5341 atgggaatcg ccggaggacg gaacccttag cgaaatttat gtcgaagaag gcggcaaggt
     5401 aaatgtcggc gacaaaatcg ctttcatcag tggggaaggc gaggaagctc cggaagaaaa
     5461 acctgaaaaa aaggacgagg ctaagaaaga gggagaagaa aaacctgtgg aagccgaggc
     5521 ggaggaaaag gaggaagccg aaatagagaa ggaagaagag aaagcgaagc ctaagaaaga
     5581 gcaagcaaaa aaggaaccgg aaaaaaagga agagaaacca aaagaagaag aaaagccgaa
     5641 ggaaccgaaa cccgaagaga aagaggaaaa aaaagccgcg ccacggaaag cagaagagaa
     5701 agctgaagct gaaaagccgg cgccagcggc cgaaaagccc gaagaagcgc gcgtaaaagc
     5761 atcgccggtc gcgcgtcgaa tcgcagccga gctcggtgtc gatcttgcca gcgtgaaagg
     5821 cacgggcccg gaaggtcggg tcaccgaaac cgacgtccgc gccgcggcaa aatcaaaagt
     5881 cgcggcgccg caaaaggcgc cgagcgcggc gaaagcagct gcgccctcta ttaaagctgg
     5941 cgaaagcgcg cgtattcaac tctccggaat gcgcaaaatc attgcgcaac ggttggtcga
     6001 aagcctcggt ccagttccgc atttttatct caacatcgac atcgacgccg ggccgctgat
     6061 ggaagcccgc gaggagttga agtccgccgg agaaggcgcg gatgctgcga aaatcacggt
     6121 aaacgatttc gttttaaaag cggcggtgca agcggcagtg aaagttccgc gcgttaatgc
     6181 ctccttcgat ggcgacgcga ttgtgcagta cgccgatgtc gatcttggca tcgcggtcgc
     6241 gattgaagac gggctgttaa caccggtcat tcgggctgcc caaaacaaat cgctgcgtga
     6301 gatcagcgag ctgggcaagg atctggcgca tcgcgcgcgc aacaagcgga tgaagccgga
     6361 agaatttcaa ggaggcacat tcactgtctc aaatctcggc ggcatgggca tcgacagttt
     6421 ctccgccgtg atcaatccgc cgcaaggatt cattctcgcc atcggcaaga tcacaaaagt
     6481 tccggtaatc gatgatggcg aacagatcgt cgtcggccac cgcatgtcga tcacgatgag
     6541 ctgcgaccac cgggtaatcg acggagcgct cggcgcggaa tatctcaaag agctccggca
     6601 tctactggag aatccggcgc tgttattggt ttgacatgcg ctgagcgcgc acgctgggta
     6661 gcgcacgcgt ctcgcgtgtt ggtttcggcg tccgcgccga aaacgaactt ttcttgaaat
     6721 gaggtttcca gacctggcac agcacaagag aagttcgcaa tcgcgaggac gcgcttgcga
     6781 ggacgcgaga cgcatgcgct acccggaaat tgtgcccccg cgcgtttccc aaatcatgaa
     6841 atcgtcgagc gtatctttcg ttctcgatac agcaaactct accaccgtag cgtcatcaac
     6901 aaatccgagg aatggaacgt tatcgggaat tagatcgaac ggatcaacga aataattgag
     6961 agcggcgatc atccacagga gcgcgtcgtc cgaaacgtct cgcggtgaat atgtgccgga
     7021 agaatacgcc tggatgaaac gcagcattgc ctggaggtat ggccagttcc ctttaaacgc
     7081 ctcgcgaggg cctgcggctg ccttcttcgc tgcctcgttg aaaagcgctt gcagactttc
     7141 cggatcgttg gcggacgatt ttgcgtcggc cagcgctttg gcgaatgctt cgctacgaag
     7201 ggcgggtttg cgatagggaa cgttttcctt aagattcttt ggagccgacc gcgtttttct
     7261 ccttctgcgc gatttctctt tggtttttct cgcctttttc atctggcgct atgaaagcac
     7321 ctcgattcgc cgatgagaag ccgattcgta gctctgggga gcacaagccg ctggcctgtc
     7381 gtcgtcggca gcttgccgac gacacggcga gtattgcgac gaggtgcaac atcgaacatt
     7441 cgaagagctt ctcggcaagc tgtcgaaaag gacagtcagc cagcctgtgc tccccagaac
     7501 acgtgccttc tacgccttcg tggcgagaat gagcggtgat ggaccgggtg cctggagttt
     7561 gcggaccttt ttgaagccgg agtcttcgag ccagtttttg atttcgttga agctgaaggt
     7621 gtccccgcgc tcgctgtgca ccagcatgtt cacagcaaag atcaatccat tcaaaggccc
     7681 ggcgcgatca tcctccacga gccattcgcc gattgcgatt gtgccgccag gcttaagggc
     7741 accgaaaact ttctggagca atttgcggct gcgctcctcg ccttcggtgt gaagaatatg
     7801 gcccagggtg gcgatatcgt agtcgttacc gaaatcggct tcaagaacgt cgccccgaat
     7861 gtaagtgaag cgatccgcca caccaaactt ctgcgtgata cgcttcgtaa ccggaatcat
     7921 ctccgcccag tcttgggccg tgacgcgaac gagcggtgat tgctgcgcga gcgcgattcc
     7981 ccaaatgccg gagcctgcgg cgacgtcgag cacgcgcatg ggcttgcgcg tcttcgcaag
     8041 gttgaggact cgaccgagcg ccgcggcggc gggataactc atcgggacga tgttttcgac
     8101 gaggacggaa aagaattccg gaccggtcgc ttcctgattt acagctaccg gcggcgcacc
     8161 cgttctcacc gcttcactca agtgtagcca atcgggcaca agatgtg
//
LOCUS       DASQUQ010000147         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_848831, whole genome shotgun sequence.
ACCESSION   DASQUQ010000147 DASQUQ010000000
VERSION     DASQUQ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848831"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..378
                     /locus_tag="VE486_04215"
     CDS             61..378
                     /locus_tag="VE486_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HZA38324.1"
                     /translation="MIHHVVLYKLKPEVTPARVEAMMMNARMQLLKIPEVLSIKCGKR
                     VDPESPWPFFIAIDFESMDKYAIFREDSVFVKFTEEIIKPNTSDSLVLDFEMDPGKDV
                     RFS"
     gene            487..2391
                     /locus_tag="VE486_04220"
     CDS             487..2391
                     /locus_tag="VE486_04220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZA38325.1"
                     /translation="MRLGYLYSRYPVISQTFCDAEMLALERRGIELVIGSVYPPLTSL
                     RHEQISRLRAPVRYAPPQEILKIWEKDAKTTGKWPGALVDQHERKYGPSFKAEQRARN
                     ALYFADLFARNGVDHFHVHFANRAAHTAIFLKEISKIPFSVTAHGQDFMKDLGNDDLL
                     REICAAAAFVAAETDYSRGLLCQRCPDSAGKIYRVYNGIDLEFFSSDASTHRDYSPDT
                     VPRIISIGRLVAFKGFDYLIDACAELTRRGLDFTCEIIGDGPLRDDLQAKIDMLKLSA
                     RVKLLGSLSQGAVIEKLRVADIFALASVTDKQGASDVFPTVIIEAMAAARPVVSTRLA
                     GIPELVVHGETGLLVAPGDTSALAEVLEQLIRDPELRSSYGRAARARIEQHFRIERTV
                     APLLELLQNTPGAPEKAIGSIAPTSIHGIAYLIDRWPDKDLPLLERELEEMKRRGVPI
                     VPFVCELDPTVRLSRSMERMAPWLEFLPDAMVIEAEWRANPALAQKLEEERAQQESRS
                     SSAIFLRQARFALVLRRLLMERKISHVHATSSRALVCALILQKISGVTVSATIEPRPE
                     LSQNWIESALGRCKGGRLSDSKLLRDRESSFLFDKVPRWRDASFWQEWAKLLGSWRRD
                     EMTEKPKLSQ"
     gene            2388..3284
                     /gene="rfbA"
                     /locus_tag="VE486_04225"
     CDS             2388..3284
                     /gene="rfbA"
                     /locus_tag="VE486_04225"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HZA38326.1"
                     /translation="MNKKAILLAGGAGTRLFPLTKIVCKQLLPIYDKPMICYPLATLM
                     LGGLREVLIITTPKDLPMLRDYLGDGTQLGIRIEYAEQPKPEGIAQAFLIGAKFIGDS
                     GASLILGDNIFYGKLDFYRDALAIERGACIFGYQVRDPQRYGVVEFDAEGKAISLEEK
                     PKQPKSHFAVPGLYVYDERVVDLCQSLRPSARCELEITDLNMLYLKEKQLHVKLLSRG
                     MAWLDTGTQTSLLEAGNYIATIEHRQGLKIACLEEVALRTGFINQSQLSAIIDRLPKG
                     EYREYLQMVCDEGPSLVPEPVG"
BASE COUNT          727 a          917 c          930 g          711 t
ORIGIN      
        1 ttgcgggcac ggattcgcac ttgcgcgctt caccggaacc gcctaaaagc tttagcttcg
       61 atgattcacc acgttgtcct ctacaaactc aaacccgagg tcaccccggc ccgcgtagag
      121 gcgatgatga tgaacgcccg gatgcagttg ctgaaaattc ccgaggtcct cagcatcaaa
      181 tgcggaaagc gggtcgatcc tgaatcgccc tggccgttct tcatcgcgat tgattttgag
      241 tcgatggaca aatacgccat cttccgcgag gactcggtgt tcgtgaagtt caccgaggag
      301 atcatcaagc cgaacacgtc ggattcgttg gtcctggact tcgagatgga tccaggcaaa
      361 gacgtccgct tctcttgaat ttcggatttc ggagcgcgga ttgcggattg taaaagagcc
      421 agcccgcatt tcatttagca cgccttcaat ccgcgatccg caacccagaa attcgaaatc
      481 atacctgtgc gcctcggata tctctattcc cggtatcccg ttatctcgca gacgttttgc
      541 gatgcggaga tgctggcgct ggaacggcgc ggcatcgaac ttgtgattgg ttccgtttat
      601 ccgccgctca ccagtttgcg gcacgaacaa atctcgaggc tgcgtgcgcc tgtccgctac
      661 gcgcctccgc aggaaatctt aaagatttgg gagaaagacg cgaaaacgac cgggaaatgg
      721 cccggcgcgc tggtcgatca gcacgagcgc aaatacgggc cgagcttcaa ggccgaacag
      781 cgcgcgcgca atgcgctcta cttcgcggac cttttcgcgc gcaacggcgt cgatcatttt
      841 catgtgcatt tcgccaaccg cgcagcgcac acggccattt tcttgaagga aatctcgaag
      901 attcctttca gcgtcacggc acacggacag gatttcatga aggacctcgg taacgatgat
      961 cttctgcgtg aaatctgcgc cgcggcggcg tttgttgcgg ctgaaacaga ttacagccgt
     1021 gggctgcttt gccagcggtg cccggattcc gccgggaaaa tttaccgggt ctacaatggc
     1081 atcgacctgg agtttttttc aagcgacgcc tcgacccacc gcgactacag cccggacact
     1141 gtgccgcgca tcatcagcat cggccggcta gttgcgttca aaggattcga ttatctcatc
     1201 gacgcttgcg cggagctgac gcgacgcgga ctcgatttca cctgcgaaat aatcggcgac
     1261 ggtccgctgc gcgacgatct tcaggcaaag atcgacatgt tgaagctctc agcccgcgtt
     1321 aaattgcttg gctctttgtc acagggagcg gtgatcgaga aattgcgagt cgccgatatc
     1381 tttgcgcttg cttcggtgac ggacaaacaa ggcgccagtg atgtttttcc aaccgtgatc
     1441 atcgaagcga tggcggctgc gcgtccggtg gtttcgactc gtcttgccgg gattcccgaa
     1501 ttggttgtgc atggcgaaac ggggttgtta gtggcgcctg gcgatacaag cgcgctggcc
     1561 gaggtgctcg agcaactgat ccgcgaccct gaattacgct cgagttacgg tcgcgccgcg
     1621 cgcgcacgaa tcgaacagca tttccgaatc gagcgcaccg ttgcgccgct tctcgaactt
     1681 ctgcaaaaca ctcctggcgc acccgagaag gcgattgggt caatcgctcc tacctctatc
     1741 cacgggatcg cctatctaat cgatcgctgg ccggacaagg atttgcccct tctcgaacgc
     1801 gagcttgagg agatgaagcg tcgaggcgtc ccgatcgttc cattcgtgtg cgaattggat
     1861 ccaacagtcc gtttgagccg gtcgatggaa cgaatggcac cctggctgga atttttgcca
     1921 gatgcgatgg tgatcgaggc agaatggcga gcaaatcccg ctctcgcgca aaaattggaa
     1981 gaggagcgag cacaacagga gagtcgatca tcaagcgcga ttttcttgcg ccaggcgcgt
     2041 ttcgccctcg tattgcgcag gctgctaatg gagagaaaaa tttcccacgt ccacgctacg
     2101 agctcccgcg cgctggtgtg cgcgctcatc ttgcagaaaa ttagcggcgt aactgtgagc
     2161 gccacgattg agccacggcc ggaattatcg caaaactgga tcgaaagcgc cttaggcaga
     2221 tgcaagggcg gccgcttgag cgattcgaaa ttgctccgtg accgcgaaag ttcatttctt
     2281 ttcgataagg tcccgcgttg gcgggacgca agtttctggc aggaatgggc caaattgctc
     2341 ggcagctggc gtcgagacga aatgaccgaa aaaccaaagc taagccaatg aacaaaaagg
     2401 ccattctact tgcagggggg gccgggacgc gtcttttccc gctcaccaag atcgtctgca
     2461 aacagcttct tccgatctac gacaaaccaa tgatttgtta tccgctggct acgctcatgc
     2521 tcggcggcct gcgcgaggtg ctcatcatta cgacgccaaa agatttaccg atgctgcgcg
     2581 attatctggg cgacggcacc caacttggca ttcgcatcga atacgccgag cagcccaagc
     2641 cggaaggaat cgcgcaggct ttcctgatcg gcgcaaaatt catcggcgac tcgggagcgt
     2701 cgctcatttt gggagacaat attttctacg ggaaactcga tttttaccgg gacgctctgg
     2761 ccattgagcg gggcgcttgc atttttggat atcaagtccg cgacccgcag cgctatggcg
     2821 tggtggagtt cgacgccgag gggaaggcaa ttagtctgga ggaaaaaccg aagcaaccta
     2881 agagtcattt cgcggtgccc gggctttatg tttacgatga gcgcgttgtc gatctttgcc
     2941 aatccctgcg cccatcggcg cgctgcgaac tcgagatcac ggatctgaat atgctctatc
     3001 tcaaagagaa gcagctccac gtgaagttgc tcagccgcgg catggcgtgg ctggacaccg
     3061 gcacccagac cagcttgctc gaagccggca attacattgc gacgatcgaa catcgacagg
     3121 gattgaaaat cgcctgtctg gaggaagtcg ccctgcgcac cggctttatc aatcagtcac
     3181 aattgtcggc gatcatcgac cgcttgccga aaggcgaata ccgggaatat ctgcaaatgg
     3241 tctgcgacga agggccaagc ctcgtcccgg agccggtggg ataat
//
LOCUS       DASQUQ010000148         7469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_207464, whole genome shotgun sequence.
ACCESSION   DASQUQ010000148 DASQUQ010000000
VERSION     DASQUQ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207464"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1612
                     /locus_tag="VE486_04230"
     CDS             <1..1612
                     /locus_tag="VE486_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZA38327.1"
                     /translation="DSLEKLASDFWTWRAKYAPFTGDDVNRMERPGGTRDWSQASIET
                     RRKDLAALEARWKKIDASGWPIPKQVDYKLIGSALARVHWELEVNPRWKRDPNFYIEQ
                     TLTPIAEALTVPAPYDEARSREILTRIDNIPSILEQAEENLANPPAPFASVAAQALEN
                     IRPRLQRMDKALLRSTTLKERELNGSVERATDALEHFRKRLQDKLPSLPAETALGRDA
                     YIFFLKNVALMPYLPEELLAMGQQEWNRAVAFEAYEKNRNKDVPPLKIAGNIHNWIKE
                     AAGKELSIRKFLQNRGNLAVPDWVQHFMLQPMPEYLRPVQDFASMVDFTSPSRLKNNG
                     SRYVTEPSPKAGYFFRATAQDPRPLTVHEGIPGHYFQLCLSWKHEDPIRRHYYDSGAN
                     EGIGFYAEEMMLQAGLFDDSPHTREIIYNFMRLRALRVEVDVKLALGEFTLEQAAKYL
                     QEKVPMGSETARQEAIAFSTGPGQAITYQIGKLQIVKFLAEARMQQGENFNLRAFHDF
                     VWKNGNVPIALQKWEYLGRDSPVISNNN"
     gene            1761..2099
                     /locus_tag="VE486_04235"
     CDS             1761..2099
                     /locus_tag="VE486_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38328.1"
                     /translation="MPSIVSRHESWLRIIVLAVLVSAMGGCGRSTIVGKWHMSGDAGA
                     TVWEFSKNGSILIGDVRGRYKFGDQNRIKIETPFATSVYQLEISGDRMILREVGGSKL
                     EFTRIREAPR"
     gene            complement(2323..3717)
                     /gene="asnS"
                     /locus_tag="VE486_04240"
     CDS             complement(2323..3717)
                     /gene="asnS"
                     /locus_tag="VE486_04240"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HZA38329.1"
                     /translation="MTQPSPTTIKDALQSSAPADAIQVQGWVRTRRDSKDFSFIELND
                     GSCLRNLQVIAKNTLPNYAALQRLTTGASVSVRGALVGSQGKGQKWEVVADKIDIVGT
                     ADDSYPLQKKGHTPEFLREIAHLRPRSNLFGCVFRVRSRLAFAVHQFFQERGFVYVHT
                     PIITGSDCEGAGELFRVTTIDCKDAPRTKGGEIDFAQDFFARQTYLTVSGQLEAEAFA
                     LALSRVYTFGPTFRAENSNTSRHASEFWMIEPEMAFCDLTGNMDLAEEIVKYLILDVR
                     KHCLEEMELFAKFVDKELLGRLDFVIDRPFQRVPYSDAVELLKKSGEKFEFPIDHGLN
                     LQSEHERWLTEKHFKCPVTVFNYPKEIKPFYMRLNDDGKTVTAMDLLVPGIGEIVGGS
                     QREERLDVLEENMRRHKMDPADYKWYLDLRRYGTAPHSGFGLGFERMLMFMTGVSNIR
                     DVIPFARTPGSAEF"
     gene            3998..4519
                     /locus_tag="VE486_04245"
     CDS             3998..4519
                     /locus_tag="VE486_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZA38330.1"
                     /translation="MAEFSVNPKRFDPYKNFKFRIKWDGRYVAGISKISGLRRTTEVI
                     QHREGGDPSSSRKSPGRTEYAPITLERGVTHDPEFENWANKVWSFGAGPGAEVSLRDF
                     RKDLTLEVYNEAGQLVLAYKIYRCWVSEYQILPDLDANATAVAIEQIKLENEGWERDH
                     SVKEPVEPSSKKS"
     gene            4626..6023
                     /gene="creD"
                     /locus_tag="VE486_04250"
     CDS             4626..6023
                     /gene="creD"
                     /locus_tag="VE486_04250"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HZA38331.1"
                     /translation="MVETQAPPPLPKSQPSFARRQSTIIKLLGVGALVLVLLIPLVMI
                     TGVLSDRLSRRDEAVADITSSWGREQNVIGPVLGIPFQYKVKAVKETPGPDGKLERRE
                     VEETAIGAAYFLPESLKIDGNVQTQTLHRGIYDAAVFRAQVNLSGKFAPPDFGPLKID
                     LKDVQWKDAFVTIAVNDLRGTREGLVLDWGGTKRPMLPGSQVPGYTTGATAFLGSDQP
                     IANEITFSIPLDFNGSDGVFFAPFGVQNEATLRSNWPDPAFRGSFLPTERSVRPDGFN
                     ATWRVSYYGRDYPQSWTSRGGNERFNVQSVTNSRFGANFLSILDAYRYVERSIKYGVL
                     FLVLVFTTFFLFEVSARQKIHPFQYLMVGAALCLFYLLLLSISEFIGFSWAYLIAAVA
                     STMLITWYCRFFLGGGARTLMIGAGLAGVYTFLYITLRQQDYALLMGAIALFIVLAVV
                     MYVTRKIDWYARDAG"
     gene            6092..6580
                     /locus_tag="VE486_04255"
     CDS             6092..6580
                     /locus_tag="VE486_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38332.1"
                     /translation="MSISFRGGFLVGLALALGIGLYLVWLWQPARQVTRHAENFLHAV
                     ERKDWAAAANFIGSDYQDQWGNDRARVLERMREGFRYVRGPRIVASNPTVQVEKRRAV
                     WTSKIMVHSTDEAAMELLDARVNSLPVPFELEWHRLSGKPWDWKLVRVSNSAFEIPAD
                     IY"
     gene            complement(6632..>7469)
                     /locus_tag="VE486_04260"
     CDS             complement(6632..>7469)
                     /locus_tag="VE486_04260"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZA38333.1"
                     /translation="RPPVAGVGLGNVIYLRTLRDALALKEMADLEKSLMVVGGGLLAC
                     EAAASLRMMKLKVGLMHRDPYLLNRYLDAETGAWLTDYFGKHGVAMSMGEGLNGFEGK
                     TILRNIQTKSGNRYPVGLAVVACGAEPNLDLVRNTPLAGPHGSPVTDYLETEEKGIYA
                     VGDLAFYPDRVMGGVRRQTHWENARKQGLIAGANMTGKKRIRYEQVPYFWTEMFDLRM
                     DFVGDFSVLPTRVDLQGTHAKKKFVARYYQGEKLRGILLCDQPPREVESAKTQLRHAL
                     GK"
BASE COUNT         1681 a         2048 c         2092 g         1648 t
ORIGIN      
        1 ggattcgctc gaaaaactgg cgtctgattt ctggacctgg cgggcgaaat atgccccgtt
       61 cacgggcgac gatgtaaacc ggatggagcg acccggcgga acacgcgact ggtcgcaggc
      121 cagcattgaa acgcgacgaa aagatctggc cgcgttggaa gcgcgctgga aaaagatcga
      181 tgcgagcgga tggccgatcc cgaaacaggt cgactacaaa ttgatcggct cagcgttggc
      241 gcgcgtgcat tgggagctcg aggtcaatcc gcgctggaaa cgcgatccga atttctacat
      301 cgagcaaaca ctcacaccca ttgccgaagc gctgactgtt cccgcgccct acgatgaagc
      361 gcgcagccgc gagattctca cccgcatcga caacatcccg tcgatcctgg aacaggcgga
      421 agaaaatctc gcgaatccgc cagcgccatt tgcgagcgtt gctgctcagg cgctggaaaa
      481 tatccggccc cgtttgcagc gaatggataa ggcgttgcta agatcgacaa cactcaagga
      541 acgggagtta aacggctccg ttgagcgggc aaccgatgct ctcgaacatt ttcggaagcg
      601 gcttcaggac aaattgccgt cgttgccggc agaaacggcg ctgggccgcg acgcttacat
      661 cttttttcta aagaacgtcg cactgatgcc gtatttgccc gaagaacttc tcgcgatggg
      721 gcaacaggaa tggaaccgcg ccgtcgcatt cgaagcttac gaaaagaacc ggaacaagga
      781 tgtaccgcca ttgaagatcg ccggcaacat ccacaattgg atcaaggagg cggcggggaa
      841 agaattgtcg atccggaaat ttcttcagaa tcgcggcaac ctcgcggttc ccgattgggt
      901 tcagcatttc atgttgcaac cgatgccgga atacctgcgc ccggtgcagg acttcgcttc
      961 gatggtggat ttcacatcgc cgtcgcgatt gaaaaacaac ggcagccgct atgtcaccga
     1021 gccttcgccc aaggcgggtt atttctttcg cgcaaccgcg caagatccgc gaccactgac
     1081 tgttcacgaa ggaattcccg gccactattt tcagctttgt ctttcctgga aacacgaaga
     1141 cccaatccga cggcattatt acgactcggg cgcaaacgag ggtatcggtt tttacgccga
     1201 ggaaatgatg ttgcaggccg ggttgttcga tgacagcccg cacacgcgcg agatcattta
     1261 caatttcatg cggctgcgcg cgctgcgcgt agaagtggac gtgaaactcg cgctcggcga
     1321 attcacgctc gagcaagccg ccaaatatct acaagagaaa gtcccgatgg gctcagagac
     1381 cgcgcgccag gaagcaattg cgttctctac cggcccgggt caggcgatca cctatcagat
     1441 cggcaaactc cagattgtga aattcctagc cgaagctcgc atgcaacagg gcgaaaactt
     1501 taatctccgt gccttccacg atttcgtttg gaaaaacggc aacgtcccga tcgcgttgca
     1561 gaagtgggaa tatcttggtc gggacagtcc tgtgatttcg aacaataatt gacgcgtaag
     1621 agcgtctaag aaaacgcagg ttgcgactga cgtcgctccg caggcggtca gtgtcgggga
     1681 cacccaaagg tgcaggcctg ttggcagaga agtcgaaggg ctagacatga caatcacggg
     1741 taattataaa atcggatgtc atgccatcta ttgtttcccg acatgagtcc tggttacgga
     1801 tcattgttct tgcagttttg gtctctgcca tgggcggctg cgggagatct actattgtcg
     1861 gtaaatggca tatgtcaggc gatgcaggtg caaccgtttg ggaattttca aaaaacggct
     1921 ccatcctcat cggcgacgtc cgcggaagat ataaattcgg cgaccagaac cgaatcaaaa
     1981 tcgaaacacc gttcgccacc tccgtttatc agctcgagat ctcaggcgat cggatgatct
     2041 tgcgagaggt cggcggttcc aagctcgagt ttaccaggat aagagaagcg ccgcgctgac
     2101 gtctgagcaa ttgcgctcga aagcggcgga acccggccgc actccaaaac gcaaagcgcg
     2161 taatgtgcac tggcgaggac ggccatgttt tggactgcgg cagtgctctg ccgctttcag
     2221 tttccttatg gagagaggag cgggcagacg atgagcaaac gactcggcag gcgggacgcc
     2281 cgccggcccc acagccaaga tggctgtgcc acgttctctc gttcagaact ctgcgctgcc
     2341 aggcgttcgc gcgaatggaa tcacgtcgcg gatgtttgag acgccggtca tgaacatgag
     2401 catccgctcg aaaccaagtc cgaaaccgga gtggggggca gtaccgtagc ggcgaagatc
     2461 gagataccat ttgtaatcgg ccggatccat tttgtgccgg cgcatgttct cctccagcac
     2521 gtcgaggcgc tcttcgcgtt ggctgccgcc gacgatttcg ccgattcccg gcaccagcaa
     2581 atccatcgcc gttaccgtct tcccgtcgtc gttcaaacgc atgtaaaagg gtttgatctc
     2641 cttcggataa ttaaaaaccg taaccggaca tttaaaatgt ttttcggtca gccaccgttc
     2701 gtgttccgac tgcaaattta gcccgtggtc gatgggaaac tcgaactttt caccactctt
     2761 tttcagaagc tcgacagcgt cactgtaagg gacccgttga aatggccggt cgatgacgaa
     2821 atcgagtcgc ccgagcaatt ctttgtccac gaacttggcg aagagctcca tttcttccag
     2881 gcaatgttta cgtacgtcca ggatcaaata tttcacaatc tcttcggcca gatccatgtt
     2941 gccggtcaaa tcgcagaacg ccatctccgg ctcgatcatc caaaactcgc tggcgtgccg
     3001 ggatgtattg gaattttcgg cgcgaaaagt tgggccgaaa gtgtaaaccc tggacaacgc
     3061 cagcgcgaac gcttccgctt cgagttgccc gctcaccgtg agataagtct gccgcgcgaa
     3121 gaagtcctgg gcgaaatcga tctcgccgcc ctttgtccgc ggcgcgtctt tacaatcgat
     3181 cgttgtgacg cgaaacaatt ctcctgcccc ttcgcaatcg cttccggtga tgatcggcgt
     3241 gtggacgtaa acgaaaccgc gctcctgaaa aaactggtga acggcgaacg caagccggct
     3301 tcgcacgcga aagacgcagc cgaacagatt cgagcgagga cgcagatgcg cgatctcgcg
     3361 caggaactcg ggcgtgtgac cttttttctg gaggggatag gaatcatcgg cggttccgac
     3421 gatgtcgatc ttatcagcta caacttccca tttttgtcct ttgccttggg acccaaccag
     3481 tgcgccgcgg acgctgacag acgcgccagt ggttaatcgc tgtaacgccg cgtaattggg
     3541 caacgtgttt ttcgcgatga cctgaagatt gcgcaaacaa gacccatcgt tcagctcgat
     3601 aaacgaaaaa tctttcgaat cgcgccgggt tcgcacccac ccttgcacct gaatcgcatc
     3661 ggctggcgcg ctgctttgca gtgcatcttt gatcgttgtt ggagaaggct gcgtcacgtt
     3721 gataatgaga gattgactcg ttcgtctccc gactctctcg ccgctaccct cgcggcgcaa
     3781 ccgtctgtgt cattcgtccc actcgctcca ttccttgacg gatcctgccc tgagcgaagc
     3841 cgaagggctc ggaacggcaa gttgcgggga accgaataag gatacccggc cggcggcgta
     3901 atctgcccgg aattgattac gattgacaga gccgagacgg tttcatatca cgcggctcgc
     3961 gcgttctttt gttcgccaac gaagacatct ccagatcatg gctgaatttt ctgtcaatcc
     4021 gaagcgtttc gatccctaca aaaactttaa gttccgcatc aagtgggatg gccgttatgt
     4081 cgccggcatc agcaaaataa gcgggttgcg ccgcacgact gaagtcattc agcatcgcga
     4141 aggaggcgat cccagcagca gccgtaaatc tccgggccgc acagaatacg caccgatcac
     4201 cctcgagcgc ggcgttacgc acgatccgga attcgagaac tgggccaaca aagtctggag
     4261 cttcggcgcc ggcccaggcg cagaagtttc acttagagac ttccgcaaag acctgacttt
     4321 agaggtctac aatgaggccg gacaactcgt tctcgcctat aaaatttacc gatgctgggt
     4381 ttcggaatat caaatacttc ccgacctcga cgccaatgcg actgctgtcg ctatcgagca
     4441 gatcaagctg gaaaatgaag gttgggaacg agaccattcc gtgaaagaac cggtcgagcc
     4501 ctcttcaaaa aaatcttgag gggacggtaa tttgtcagac gtgcaagttt cctctggtcc
     4561 cgcggcaaag agcggtcgaa acaagttttg ttgtccaatc gagtcaactc attcaatctg
     4621 ccgccatggt tgaaacacaa gcgccgccgc ccttgccaaa gtcgcagcca tcgtttgccc
     4681 ggcggcagag cacgattatc aaattgctcg gggtcggcgc ccttgtgctg gtgttgttga
     4741 ttccgctggt aatgattact ggcgtcttga gcgatcgact ttcgcgccgg gacgaagcag
     4801 tggcggacat tacttcatcg tgggggagag agcagaatgt gatcgggccg gtgctgggta
     4861 tcccgtttca atacaaagtt aaagcggtga aggaaacgcc ggggccggac gggaaactgg
     4921 agcggcgcga ggttgaggag acggcgatcg gggccgcgta ttttctgccg gaatcgctca
     4981 agatcgatgg caacgtccag acgcagacat tgcatcgcgg catctatgac gcggcggtgt
     5041 tccgcgcgca ggtaaacctg tcgggaaaat ttgcgccgcc cgattttggc ccgctcaaga
     5101 tcgacctgaa ggatgtgcaa tggaaagacg cattcgtgac gatcgcggtg aacgatctgc
     5161 gcggcacgcg cgaaggattg gtgctcgatt ggggcggaac gaaacggcca atgctgccgg
     5221 gatcgcaagt accgggttac acaaccggcg cgactgcatt cctcggcagc gaccagccga
     5281 tcgcgaatga aattacattt tccattccgc tcgattttaa cggcagcgat ggagtttttt
     5341 tcgcgccgtt cggtgtgcaa aatgaagcga cgttgagatc aaactggccc gacccggcgt
     5401 ttcgcggctc gtttctgccg acggaacgat cggtgcggcc ggatggattt aacgcgacct
     5461 ggagagtttc gtattacggc cgcgattatc cacagtcgtg gacaagtcgc ggcgggaacg
     5521 aacggttcaa tgttcagtcg gtaacaaact caagattcgg cgcgaatttc ttgtcgatcc
     5581 tggacgcata tcgctacgtt gagcgctcga tcaagtacgg cgtgcttttt ctcgtgctcg
     5641 tttttacgac atttttcctg ttcgaagtca gcgcgcgcca aaaaatccat ccgttccaat
     5701 atttgatggt gggcgcggcg ctttgcctgt tttatttgct gctgttgtcg atttcggaat
     5761 ttatcggatt cagttgggct tatttgatcg cggcagttgc atcgacaatg ttgatcacgt
     5821 ggtattgccg cttttttctc ggcggcggcg cccgcacgtt aatgattggc gccggcctgg
     5881 cgggcgttta cacgttcctt tacattacgt tgcggcagca agactatgcg ttgcttatgg
     5941 gagcgatcgc cctcttcatc gtactcgcgg ttgttatgta cgtgacccgc aagatagatt
     6001 ggtacgcgcg cgacgcggga tagatgtgga ttgaccgcat attctgcgag ccaaatggcg
     6061 taatctgcgg gagagaataa aatcgcagtc tgtgtcgatc tcgtttcgtg gaggattcct
     6121 ggttggtctg gccctcgcgc ttggcatcgg gctatatctc gtttggctct ggcagccggc
     6181 gcgccaggta acgcgtcacg cagaaaactt tctccatgcc gttgagcgca aagactgggc
     6241 ggcagccgcc aattttatcg ggagcgatta tcaggatcag tggggcaacg atcgagcgcg
     6301 cgttctggag cgcatgcgtg aaggctttcg atatgtgcgc gggcccagga ttgtcgcatc
     6361 caacccaact gtacaggtcg aaaagcggcg cgcggtgtgg accagcaaaa tcatggtcca
     6421 tagcaccgac gaagcagcga tggagttgct cgacgcacgc gttaactcac tgcccgtgcc
     6481 tttcgagctg gagtggcatc gcctctcggg caagccgtgg gattggaaac tggtgcgagt
     6541 gagcaattcg gcttttgaaa tcccggccga tatctattag cgccgacttt cgcgagtgag
     6601 agatcggcgc ttatagaaca ccgctaccag ctcatttgcc cagtgcgtgg cgcaattgtg
     6661 tcttggccga ttccacctcg cgcggaggtt gatcacagag cagaatgccc cgcagcttct
     6721 caccctgata atagcgcgcg acgaattttt tcttcgcgtg cgtgccttga agatcgacac
     6781 gagtgggcag cacgctgaaa tcgccgacaa aatccatccg taaatcgaac atctccgtcc
     6841 agaagtaggg aacctgctca tagcggatgc gttttttgcc cgtcatgttt gcgccggcga
     6901 tcaagccttg tttacgcgcg ttctcccaat gggtctgacg gcgaacgccg cccattactc
     6961 gatctggata aaatgccaga tccccgaccg cataaattcc cttttcttcc gtctcgagat
     7021 agtccgtgac tggcgaacca tgtggtccgg cgaggggtgt attgcgcacc agatcgagat
     7081 tcggttcggc tccgcaggcg acaacagcga ggccaacggg gtatcggtta ccgctttttg
     7141 tctggatatt gcgcaggatg gttttgccct caaagccatt gaggccttct cccatcgaca
     7201 ttgccacccc gtgtttccca aaataatccg taagccacgc gcccgtttca gcgtcgagat
     7261 agcggttcaa caaataagga tcacgatgca ttagaccaac cttcagcttc atcattcgca
     7321 aactcgctgc cgcctcgcat gcgagtaacc cgccgcccac gaccatgagg ctcttctcca
     7381 gatcagccat ctcctttaac gcgagcgcgt cacgaagggt tcgcagatag atcacattac
     7441 cgagaccgac tccagcgacc ggcggtcgc
//
LOCUS       DASQUQ010000149        12211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_119776, whole genome shotgun sequence.
ACCESSION   DASQUQ010000149 DASQUQ010000000
VERSION     DASQUQ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119776"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VE486_04265"
     CDS             <1..279
                     /locus_tag="VE486_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38334.1"
                     /translation="DLGSGEKKAVRQQLFKGNEYWFWLGTEVERAKVSVHIYDSDGKL
                     AEEPDSWQKGHFAATHVIPRATGSYFIIVSVEESPEERTHWALVYGFR"
     gene            358..1332
                     /locus_tag="VE486_04270"
     CDS             358..1332
                     /locus_tag="VE486_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZA38335.1"
                     /translation="MTETRTLQFESPRALQSLYANDLKLLKSLEDTFGLKVTTREGWV
                     RLEGEPGGIDKAQHVFEQLEKARQKGVDIQKHEFNYALNSVTEARDENLGDLASTKIV
                     TSPRKSPIIARSAGQRNYVEAIQKHDIVFGIGPAGTGKTYLAVAMAVAALKKDQVARI
                     ILARPAVEAGEALGFLPGELEQKIMPYLRPLYDALRDMLEPEEIERLLARQTIEVAPL
                     AYMRGRTLNHAFVILDEAQNTTTEQMFMLLTRIGPNSKCVITGDVTQIDLPANKRSGV
                     VEALQALKTIPGIATVYFTERDVVRHELVRAIISAYQQHRSPAPGSRK"
     gene            1342..2982
                     /locus_tag="VE486_04275"
     CDS             1342..2982
                     /locus_tag="VE486_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HZA38336.1"
                     /translation="MFDFSRRARLIRRGLASRKMRRRRHASELARNLEFAPYAKFLIF
                     AAFVGGLAFLVFSGQQPEPTKNFVIALLVLATAITQLWINQPKSFSRSSRILLIFGVI
                     LVQLAVTKGLLVICNSGNYWFLKPETGGLITPYAFAPLVLSVLLGRHHGLYAAFFVSL
                     WSSVLFGPIDAPLLVTSLISGFTAVSLTLQVRRRSKLIRAGLGVGVAIWLLSLTFGLI
                     GPITLFSPIANDWGMIGLQSALAIGNGVLTATLVGGALPMLEHLFRITTDISWLEASD
                     LNHPLMRRMTIEAPGTYHHSLVVANLAEAAAEAIGANATLCRVCSYFHDVGKLVKPEY
                     FTENMSFERNPHDDLAPTMSALIIIAHVKEGVDLALKHKLNQRIIDIIQEHHGTSLVR
                     YFYQRALQQHEDARAGGKIMKLREDDIPEVREESFRYGGPKPQTRESAIVSLADTVES
                     ASRSLEKPTPQKIESLVNELIEERITDRQLDDCDLTLGELKVIAERFRFTLLMMLHSR
                     IAYPKPGQKPAGPREEALRPDVMAATRRPATAPPVSAA"
     gene            complement(3316..3891)
                     /locus_tag="VE486_04280"
     CDS             complement(3316..3891)
                     /locus_tag="VE486_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZA38337.1"
                     /translation="MTGQELFNSLREAFEPKIQDRTEFRDETTCTIAAGDLREIAKFC
                     RDELSFDYLIDITSIDNFGEEPRFEIVYELYSMPNAIHLRLKLRVSEDVGAIDTVSDV
                     WPTANWHEREIYDMMGIKFNGHPDLRRILMWDGYPFFPLRKEFPLEGLPSEMPDVAFT
                     KVTSMEGGPFVTRPTTATSKDREPRARPPEL"
     gene            complement(3990..4526)
                     /gene="nuoB"
                     /locus_tag="VE486_04285"
     CDS             complement(3990..4526)
                     /gene="nuoB"
                     /locus_tag="VE486_04285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF011390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HZA38338.1"
                     /translation="MPETHSTAYDSKIEGNVIFTRFDAALNWVRKNSLWPMPMGLACC
                     AIELMATAASRFDIARFGAEVMRFSPRQCDVMIVAGTVTYKMALAVKRIYEQMPEPKW
                     VIAMGACASTGGMYRSYAVLQGIDQLLPVDVYISGCPPRPEALLAGLMKLQEKISGER
                     SFAKQKPELVEKLKEALA"
     gene            4720..5538
                     /gene="cysD"
                     /locus_tag="VE486_04290"
     CDS             4720..5538
                     /gene="cysD"
                     /locus_tag="VE486_04290"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HZA38339.1"
                     /translation="MTETTRTHLEQLENQSIFIFREAYHAFKNVAMPWSMGKDSNVLI
                     WLARKSFFGKVPFPVLHIDTTYEFPEMLEFRDWATAHYNLNLIVKINTEARERGIGYE
                     THDPVTVTHELKTVALQQALADYKWDALITGIRRDEDPTRAKERYFSPRNAQFEWDYK
                     DQPPEFWGQFATSAAKGEHVRVQPLLDWTEVDVWRYIQKEEIPIPKMYFARNGKRFRS
                     LGCSPITHPVASTASTIDEIIAELEATKTTERAGRAQDSYERNAMQKLRAKGFL"
     gene            5535..7442
                     /gene="cysC"
                     /locus_tag="VE486_04295"
     CDS             5535..7442
                     /gene="cysC"
                     /locus_tag="VE486_04295"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR02034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HZA38340.1"
                     /translation="MIAEGNAQRLGSDTAPKLRVVFVGHVDHGKSTLIGRILHDTGSL
                     PDGKIDEIKKACAAEGMEFEFAFLLDALLEEQKQNVTIDTTQIPFRTARRRYAIIDAP
                     GHKEFLKNMITGASSADAAILVIGANEGVREQSRRHAYLLNLLGIKQIIVIVNKMDLV
                     DFSEVLFHEIERDYGKFLLSLGLEARAFIPASAKQGENVARATMKMKWYSGATVLEAL
                     DLLQSQKPDVDLPLRFCVQDVYRFDVRRIIAGRIETGRLRVGDQLVFSPANKSSVVAT
                     IESWNAPANGPAVAGDSVGITLSEQIFVERGYVASHESETPIETNRFHADLFWIVREP
                     LRLDHVYDLRLATQNVKCQIVSIEQVIDSSTLETKSAGREQLERNEIGRLTIQTRGPL
                     VIDNHDRIPNLGRFVIVDDGRICGGGTIFGGVYTDRKAAKSKNIFWTEGKITARQRAL
                     RSGHRGAVLWLTGLSGAGKSTIAQALERELFQRAMHTYVLDGDNIRHGLNSNLGFSPE
                     DRVENIRRVSEVAKLMADAGAVVITAFISPYRMDRRRAREIALEGNAEFIEVFVDAPL
                     EVCETRDPKDLYKKARAGEIRDFTGIGAPYEAPEDPEIVVRTDQQTVDESVATILEQL
                     LPRLREYGAEE"
     gene            7456..7905
                     /locus_tag="VE486_04300"
     CDS             7456..7905
                     /locus_tag="VE486_04300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZA38341.1"
                     /translation="MALTIRDAKLSDAPQLAALMCELGYETAGDEMSARLKAILPDAR
                     CSTFVATIGTEVCGMIGTLTHMSHEHNDLSGKIVALVVSKKQRRSGVGRALIAAAEKD
                     FARRNVTRVTLTTRFEREEAHRFYAELGYSRTGFRFAKNLARDFQRM"
     gene            complement(8013..8789)
                     /gene="truA"
                     /locus_tag="VE486_04305"
     CDS             complement(8013..8789)
                     /gene="truA"
                     /locus_tag="VE486_04305"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZA38342.1"
                     /translation="MSRRLKMIVAYDGKGFAGWQSQSHRNTIQDHIERAFECIGGKPF
                     RIHGAGRTDAGVHALAQCAHVDLPDKRLSAARWTEAVNALLPPAIRVLRCRYVAKDFH
                     ARLSAKGKLYRYRIWSGPVLPPFEYGRAWHVAGPLDLEVLETAAKHFVGTHDFASFAA
                     NRGKSGNRTASSRGEREKNTIRTIYSLRVRRNRPCITLDFDGDGFLYKMIRLIVGSLV
                     KCALGKLRVEDLTDRLSSSQGSQARFTAPAEGLFLVRVRY"
     gene            complement(8817..9254)
                     /gene="ruvX"
                     /locus_tag="VE486_04310"
     CDS             complement(8817..9254)
                     /gene="ruvX"
                     /locus_tag="VE486_04310"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZA38343.1"
                     /translation="MNPILALDLGRARIGVAISDELQLLAHPLETIPANDQATSRLAQ
                     IIREKKVEQVVVGIPRQMNGQIGPAATEALRFVEKLRAILPCPVVTWDERLTTVAAHR
                     ALRDAGKKTRDTRAYVDQVAAQMILQGYLDRRAASAHSPITDH"
     gene            complement(9267..10340)
                     /locus_tag="VE486_04315"
     CDS             complement(9267..10340)
                     /locus_tag="VE486_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZA38344.1"
                     /translation="MLNPTYVLILAGGSGERFWPLSRRARPKQLLRLVSDKTLLEETV
                     ARLEGFVSHKRILILTNAEQEAAVRKLVSGFPKENILAEPAKRDTAAAVALGTGWVAA
                     RDRAATMIVLPADHVIANRAAFQETLALAVDAAEETGELVTIGIRPTWACPGFGYIEH
                     GEPVHLRKSNDKSAIHRVVRFREKPNPDLAESFLRKGNFRWNAGMFVWSVPTVLSEFN
                     RRAPELADFISQLRAQGKFEKALRERFGKLPRISFDYAIMEKADRVLVVEASFDWDDI
                     GSWRAVANYFEKDEQGNAANCAVTAVDSTNNVIFDEDGTTIALLGVHNLIVVRTGDAV
                     LICHRHQAEKIKDLIGKLPPKLQ"
     gene            10424..10726
                     /locus_tag="VE486_04320"
     CDS             10424..10726
                     /locus_tag="VE486_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38345.1"
                     /translation="MSLPISISKTAEQLPIDPLLVVTRAAIGLGVGLLMADKIKPSIR
                     QVAAIALVSIGALAAAPWLVKITLGQINRPESEWGSRARLRSIRGDSGYSSDNDVY"
     gene            complement(10763..>12211)
                     /locus_tag="VE486_04325"
     CDS             complement(10763..>12211)
                     /locus_tag="VE486_04325"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZA38346.1"
                     /translation="GEVFAYTDSDCMADVDWLYYLTATLVNGDYAGVGGPNVTPPAQN
                     WIQACVAAAPGGPSHVLLTDTVAEHIPGCNMAFHRWAFESMGGFDPEYRKAGDDVDFC
                     WRLQQAGFVIAFSPTAIVWHHRRFTLRAFLNQQQGYGEAESLLRFKHLIFFGPTGTAK
                     WRGQIYGAPRPVSFINRPVIYHGIFGEGFFQSIYPTPQSEVAAYLSSIEWFALTVFLF
                     GLGILLPALRIVPYLMIGGTLCVALSYMVRAPIEPKFDTVHARLLVMLLAFVQPLVRG
                     WSRYFTWLHFKRTPRSVIRAHEHLPNGVRVAGSLSRRVFWSEEGRDRHYLLGAIFQLL
                     DEEGWRYSTDSGWNEWDIQIYGNFWWSIALQTVTEYHGGGKCLTRVRLRDRLVTTTVI
                     FNLIAVSLLVYRQLNVPHIDFWFTIPYLLFLGFLATRARVLKSRVAELVELAARRVGL
                     QRVPRRGRIAVNELTPQVEVPADAMDSGVSRP"
BASE COUNT         2812 a         3387 c         3404 g         2608 t
ORIGIN      
        1 gacctcggtt ccggggagaa gaaagccgtg cggcagcaat tgtttaaagg gaacgaatac
       61 tggttttggt taggcacgga agtggagcgg gcgaaggttt ccgttcacat ctacgattcg
      121 gatgggaagc tggccgaaga accagacagc tggcagaagg gtcacttcgc cgccacgcat
      181 gtcattccaa gagcgaccgg cagctacttt atcattgtca gcgtcgagga atcgccagag
      241 gaacgcacgc actgggcgct ggtgtatggt ttccggtagg cgtacatgtc gatcttgcag
      301 tggcatgcgc ttccggcgaa tatttcacgg tcaggatgcc gatgccacgg ttgagccatg
      361 acggaaacgc gcaccttgca atttgaaagc ccgcgtgctc tgcaatcact ctacgccaac
      421 gatcttaaac tcttgaaaag ccttgaggat acctttggct tgaaggtgac gacgcgcgaa
      481 ggctgggtaa gactggaagg ggagccaggc gggatcgaca aagcgcagca tgtgtttgag
      541 caacttgaga aggcgcgcca aaaaggcgtg gacattcaaa aacacgaatt taattacgct
      601 ttgaattcgg tgaccgaagc gcgcgatgag aatcttggtg atcttgcctc gacgaaaatc
      661 gtcacctcgc cgcgcaagtc gccgatcatc gcgcgatcgg ccgggcagcg taattacgtc
      721 gaagcgatcc aaaagcacga catcgttttt ggaatcggcc cggccggcac cggtaagact
      781 tatctcgccg ttgcgatggc tgttgctgcg ttgaaaaaag accaagtagc gcggatcatc
      841 ttagccaggc cggccgtgga agctggcgaa gcactcggat ttttgccggg cgagctggaa
      901 caaaaaatca tgccctacct acggccgctt tatgatgcgt tgcgcgatat gctcgaaccg
      961 gaggaaattg agcgtctgct ggcccggcag accatcgagg tcgctccact tgcttacatg
     1021 cggggccgca ccctgaacca tgcctttgtc attctcgatg aggcgcaaaa cacgacgacg
     1081 gagcaaatgt tcatgttgct gacccggatc ggtccgaact ccaaatgcgt gattacggga
     1141 gacgtgaccc aaatcgattt gccggcgaac aaacgttcgg gtgtcgttga ggcactccag
     1201 gcattgaaaa ccattcccgg aatcgcgact gtttatttta ccgagcgcga cgtggtgcgg
     1261 catgaactgg tccgcgccat tatcagcgcc taccagcaac accgctcgcc cgcgccggga
     1321 tcacgcaagt aataaatctg catgttcgat ttttctagac gcgcacgcct tatcaggcgc
     1381 ggccttgctt cgcgcaagat gcggcgacgc cggcatgcca gcgaactggc gcgcaatctg
     1441 gaattcgccc cgtatgcgaa gtttctaatc tttgccgcat ttgtcggcgg cctcgctttt
     1501 ctcgtcttca gcggccagca accggagccg acgaaaaatt tcgtcatcgc gctgctggtt
     1561 ttggccaccg ctattactca actctggatc aaccagccaa agagtttttc gcggagctcg
     1621 aggattctcc tcatctttgg ggttatcctc gtgcagctgg ccgtcaccaa aggcttgctg
     1681 gtgatttgca acagcgggaa ctactggttt ttaaagcctg aaacgggcgg attaatcacg
     1741 ccttatgcct tcgcgccact ggtgcttagc gttttgctgg gtcgccacca tggtctttac
     1801 gccgcatttt ttgttagtct ctggagtagc gttttgtttg gtcccattga cgccccgctt
     1861 ttggtaacca gtctgatcag cggattcacc gcagtctctc tcacgttgca ggtgcgacgg
     1921 cgcagcaaat tgatccgcgc aggcctcgga gtcggggtcg ccatttggtt gttatcgctg
     1981 acgtttggct tgatcgggcc gattactctc ttttcgccga ttgccaacga ttgggggatg
     2041 attggattgc aaagcgcgct cgcaatcggc aatggagtct tgactgccac tctggttggc
     2101 ggcgcgctgc cgatgctcga acatctattt agaatcacaa ccgacatttc ctggctggaa
     2161 gcctccgatc ttaaccaccc gttgatgcgc cgcatgacca tcgaggcgcc gggcacctat
     2221 catcacagcc tcgtcgtggc gaatctcgct gaagcagccg cagaagcgat tggcgcaaac
     2281 gcgacgctct gccgcgtctg ttcttatttt cacgacgtcg gcaaattggt gaaaccggaa
     2341 tacttcacag aaaatatgag cttcgagcgt aacccccacg acgatctcgc cccgacaatg
     2401 agcgcattaa tcattatcgc gcacgtcaag gaaggggtcg atctcgccct gaagcacaag
     2461 ctcaatcagc gcatcatcga catcattcag gaacatcacg gcacttcact cgttcgttac
     2521 ttttatcagc gtgcccttca acagcatgaa gacgcgcgcg caggcggcaa aatcatgaag
     2581 ttgcgcgaag acgacattcc cgaggtgcgc gaggagagtt ttcgctatgg cggacccaaa
     2641 ccgcagacga gggaaagcgc gatcgtcagc ctggcggata cggtcgagag cgcctcgcgc
     2701 agtctggaaa aaccgacccc gcaaaagatc gaatcgcttg tgaatgagct catcgaagag
     2761 cgcattaccg atcggcaatt ggacgactgc gatctgaccc tgggcgagct gaaagtcatc
     2821 gccgagcgtt tccgattcac gctcctgatg atgttgcaca gtcgcatcgc ttatccgaag
     2881 cccgggcaaa agcccgccgg accgcgagag gaagccctgc gtccagacgt gatggccgcc
     2941 acgcgaaggc ccgcaaccgc tccgccagtc tcagccgcgt aagggcaggg cgcgaccggc
     3001 gggcgcgccg acgaagctgg cagtggtctt cgcggaaaac taaagctggt agggcgagac
     3061 tctgtcgagc cgacgaaact ttaaccaaga atcagagcga tgcctcgctg gtgtggtcgc
     3121 caacccgcaa catcatccca gttaatatcg tatgtaaatt ggtttggcca tgtggcgagg
     3181 aaacgatctt gccttgagca ggcagttcaa ggagaaatgt agtgatcgca cggaagcatt
     3241 tgtgcgttag gtctattcgt agcaacacga attttcttgc tgattcgtcg actcgacgga
     3301 gtctcgccct accattcaca attccggcgg gcgagcgcgg ggttcgcgat ccttggaggt
     3361 tgccgtggtt ggcctggtga cgaatggtcc gccttccatc gatgtgacct tcgtgaaagc
     3421 gacatctggc atttcgctcg gcaatccttc gagcggaaat tcttttcgca aggggaagaa
     3481 tggatagcca tcccacatca atatgcgtcg cagatcggga tggccgttaa acttgattcc
     3541 catcatgtcg taaatctccc gttcgtgcca gttggccgtt ggccagacat cggaaaccgt
     3601 atcgatcgca ccgacatctt ccgagacgcg caatttcaat cgcagatgaa tggcgttcgg
     3661 catcgaatac aactcgtaaa cgatttcgaa acgcggttcc tcgccgaaat tgtcgatgct
     3721 ggtgatgtcg atcagataat cgaatgaaag ttcgtcacgg cagaactttg cgatttcccg
     3781 aaggtcgcct gccgcaatcg tgcaggtcgt ttcgtcccga aattcggtcc tatcctgaat
     3841 ctttggttca aacgcttccc ttaacgagtt gaacagctcc tgaccagtca tgcgctatgc
     3901 tgttgtaggg caggcgctcc gcctgccaag accatcgcag aaggcaagcg atgcgcttgc
     3961 cctacaattg gaaattccag cgcacgtaat catgcgagcg cctccttgag tttctcgacc
     4021 agctccggtt tctgtttcgc gaacgagcgc tcgccggaaa ttttctcctg caatttcatc
     4081 aagccggcca gtaatgcttc cggacgcggg ggacagccgg aaatgtaaac atcgaccggc
     4141 aaaagttgat cgatcccctg gagaacggcg taggagcgat acatgccgcc ggtcgaggcg
     4201 cacgcgccca tcgcgatcac ccatttcggc tccggcattt gctcgtaaat tcttttcact
     4261 gccagcgcca ttttgtaagt cacggtgccg gcgacgatca tcacgtcgca ttgtcgcggc
     4321 gagaaacgca ttacttccgc gccaaaccgc gcaatatcga agcgcgacgc tgcggtcgcc
     4381 attaattcaa ttgcgcaaca ggcaagcccc atcggcattg gccaaagcga attttttcgc
     4441 acccaattga gcgctgcgtc gaagcgcgta aaaatcacgt taccctcgat tttggaatcg
     4501 taagccgtag aatgcgtttc cggcatggtt caacgttaaa atacgacact gaccacgcaa
     4561 gccgaatccc gcgcgcaacg accaacaatc agatttgagg taggggcgat ttgacctcaa
     4621 tcgcccgctc ggtaattgtc atcactcgtg gaaaggcggt tcgggtctgc cgcgccgtag
     4681 cacagcaaag gcggctgaac ccgcccttac ctgaaagata tgaccgaaac gacccgcacc
     4741 catctcgaac aactcgagaa ccagagcatc tttatttttc gagaggctta tcacgccttc
     4801 aaaaacgtcg ccatgccgtg gtcgatgggg aaggattcca atgttctcat ctggctcgcg
     4861 cgcaaatcct tctttggaaa ggtgccgttt ccggttctgc atatcgacac gacctacgag
     4921 tttcccgaga tgctggaatt tcgggattgg gcgaccgcgc attacaacct gaatctgatc
     4981 gtcaaaatca ataccgaagc tcgcgagcgc ggcatcggtt acgaaacgca cgatccggtg
     5041 actgtgacgc atgagctgaa gaccgtggcg cttcagcaag cgctcgccga ttacaaatgg
     5101 gacgcgctca tcaccggtat tcgccgggat gaagatccga ctcgcgcgaa ggagagatac
     5161 ttttcaccgc gtaacgcaca gttcgaatgg gactacaaag atcagccgcc ggagttttgg
     5221 ggacagttcg cgacttcagc tgcgaaaggg gaacacgttc gcgtgcaacc gttgctcgat
     5281 tggaccgagg ttgatgtctg gcgctacatc caaaaggagg agattccgat cccgaaaatg
     5341 tatttcgcgc gaaatggaaa acggtttcgt tcgctcgggt gcagcccgat tacgcacccg
     5401 gtcgcgagca ctgcatcgac aatcgacgaa attatcgccg agctcgaagc gacaaaaacg
     5461 accgagcgcg ccgggcgggc ccaggactct tacgagcgca atgcaatgca gaagttgagg
     5521 gccaaaggat ttctatgatc gcggaaggaa acgctcaacg tctaggttca gacaccgcgc
     5581 ctaagctccg cgtggttttc gtcggtcacg tcgatcatgg aaagtccacg ctcattggcc
     5641 gcattctgca tgacaccggt tcacttcccg atggcaagat tgatgaaata aaaaaggcct
     5701 gcgcggcgga gggaatggaa ttcgaatttg cgttcctgct cgatgcgctg ctcgaagagc
     5761 aaaagcaaaa cgttactatc gacacgactc aaatcccgtt tcggacggcg cggcgtcgct
     5821 acgccatcat cgacgcgccg ggtcacaagg aatttttgaa aaacatgatt accggagcgt
     5881 cgagcgccga cgctgcaatc ctggtgatcg gagccaatga aggtgtgcgc gagcaaagcc
     5941 ggcgccatgc ctatttgctc aacctgctcg gcatcaagca gatcattgtg atcgtaaaca
     6001 aaatggatct ggtggatttt tctgaagttc tgtttcacga gatcgagcgg gattacggaa
     6061 agttccttct cagtctcgga ctggaagcgc gcgcgttcat tccagcctct gcaaagcaag
     6121 gggaaaacgt cgcgcgcgcg accatgaaaa tgaaatggta ctccggtgcg accgttctgg
     6181 aggccctcga cctgttgcag tcgcaaaaac ccgatgtcga tcttccgctg cgcttttgcg
     6241 tccaggatgt gtatcgcttc gatgtacggc gaatcattgc gggccggatc gagactggaa
     6301 ggttacgggt gggggaccag ttggtatttt cgccggcaaa caaatcctcg gtggtcgcca
     6361 ccattgaatc ttggaacgcg ccggcaaacg gccctgccgt tgcgggggac tccgttggaa
     6421 tcacattaag cgagcagatt ttcgtcgaac gcggctatgt tgcatcgcac gaaagcgaaa
     6481 caccgatcga gacgaaccgt tttcatgcgg atctgttttg gatcgtgcgc gaaccattgc
     6541 gccttgatca tgtttacgat ctgcgcctgg cgactcaaaa cgtgaaatgc cagatcgtat
     6601 cgatcgagca ggtaatagat tcgtccaccc tcgagaccaa gagcgccggg cgcgaacagt
     6661 tagagcgaaa cgagattggc agactgacaa ttcagacccg gggcccactc gttatcgaca
     6721 atcatgatcg catcccgaat ctcggccgat ttgttatcgt ggacgacgga cggatctgcg
     6781 gtggcggcac gattttcggc ggtgtttata ccgaccggaa agctgccaag agcaagaata
     6841 ttttttggac cgagggaaaa attaccgccc gccagcgcgc attacgaagc ggccatcgcg
     6901 gagcggtcct ttggttgacc gggctttccg gtgctggaaa atcgaccatt gcccaggcac
     6961 tcgagcgaga gttgttccaa cgcgccatgc atacctatgt cctggatggt gacaacatcc
     7021 ggcatggttt gaattcaaat cttgggttct cgcccgagga ccgagtggag aacatccgcc
     7081 gggtgagtga agtcgcgaag ttgatggccg acgccggcgc ggtcgtcatt acggcgttca
     7141 tctcaccgta tcgaatggat cgccgccgcg cgcgcgaaat tgcgcttgag ggtaatgccg
     7201 aatttatcga agtcttcgtg gatgcgccgc tcgaagtgtg cgaaacgcgg gacccaaagg
     7261 atctctacaa aaaagcgcga gcgggtgaaa tccgcgattt caccgggatt ggtgcaccct
     7321 acgaggcgcc ggaggatccg gaaatcgtgg tacgcactga tcagcaaact gtcgatgaat
     7381 ccgtggcaac gattctggag caattattgc cgcgtctgag agaatacgga gcggaagagt
     7441 gacgctccgg tagttgtggc gctaacaatt cgggatgcga aattgagcga tgctccgcag
     7501 cttgctgcgc tcatgtgtga actcggctac gaaacggcgg gcgacgaaat gagcgcgcgt
     7561 ttgaaagcta tcttgccgga tgcgcgttgc agcaccttcg tcgctacgat tggcacagag
     7621 gtttgtggaa tgattggtac gcttacacac atgagccacg aacataatga tctttccgga
     7681 aaaattgttg cgctggttgt ttcgaagaaa cagcgccgca gtggcgtcgg gcgcgctctg
     7741 attgcggcgg cggaaaagga tttcgcgaga agaaatgtta cccgagtgac gctaaccacg
     7801 cgattcgaac gcgaagaagc gcatcgattt tatgcagagc tcggctattc gcgaacaggt
     7861 ttccggtttg caaagaacct cgcgcgtgac ttccaacgca tgtagggacg cacctctggg
     7921 gcgtccgact tattgttcct ggatttggct tggagaataa cgatcaagtc cgtcggacat
     7981 ctgggagaga tgtcccctta cttgttaaat catcagtagc ggacgcggac gaggaacagg
     8041 ccttcggcgg gtgccgtaaa gcgtgcctgg ctgccttgac tggaagagag gcggtcagtc
     8101 aaatcctcga cacgcaattt gccgagcgca cacttgacaa gcgagccaac gatcaatcga
     8161 atcatcttgt agagaaagcc gtcgccatcg aaatcgagcg ttatacaagg tctattacgg
     8221 cgcacgcgta gagaatagat cgtccgaata gtgttctttt ctcgctcgcc gcgggacgag
     8281 gccgtccgat tgccggattt gccgcgatta gcagcgaagc tggcaaaatc atgcgtgcca
     8341 acgaagtgtt tggcggctgt ttccagaact tcgagatcga gcgggcccgc gacatgccag
     8401 gcgcggccat attcgaatgg cggcagcacc gggccagacc agattctgta gcgatagagt
     8461 tttccttttg cggaaaggcg cgcatgaaaa tcctttgcca catatcgaca tcgaagcacg
     8521 cgaatggctg gcggcagcaa agcatttacc gcttccgtcc aacgcgcggc cgaaagacgc
     8581 ttatcgggga gatcgacatg ggcgcactgg gcgagagcgt ggacgcctgc atccgttcgc
     8641 ccggccccat gaatacggaa gggcttgccc ccgatacact caaacgcgcg ctcgatatga
     8701 tcctgaatcg tgttgcggtg ggattgactt tgccagccgg caaatccttt cccgtcatag
     8761 gcgacaatca tcttcagccg tcgcgacatg gcagcccaat gattcgtgat cggtgatcag
     8821 tgatcggtaa tcggtgagtg cgcgctcgcc gcgcggcgat cgagatatcc ctgcaaaatc
     8881 atctgggcag ctacttgatc gacatacgca cgggtatcgc gcgttttctt gccagcgtca
     8941 cgcagcgccc gatgcgctgc aacggtggtc aaccgttcgt cccatgtgac gaccgggcat
     9001 ggaagaatcg cccgcaattt ttctacgaac ctgagggcct cggttgcggc ggggccgatc
     9061 tgaccattca tctgtcgcgg gatccccaca acaacctgct cgactttctt ttcgcgaatg
     9121 atttgagcca atctcgatgt cgcctggtcg tttgctggga ttgtttccag cggatgcgct
     9181 aataattgta actcgtcgga gatcgccact ccgatgcgcg cgcgcccgag atcgagagcc
     9241 aaaattggat tcatcgcgtc ctcagatcat tgcagtttcg gcggcagctt gccgattaag
     9301 tccttgatct tttctgcctg gtgacgatga caaatcaaaa ctgcatcgcc agttcgtaca
     9361 acaataagat tatgaactcc gagcagggcg atcgtcgtcc catcctcatc aaagatgacg
     9421 ttgttggtgg aatcgaccgc agtaaccgca cagttggcgg cattgccctg ttcatctttc
     9481 tcgaagtaat tcgctaccgc ccgccagctt ccgatgtcgt cccaatcgaa gctcgcttcc
     9541 accaccagca ctcggtccgc tttttccatg atcgcgtaat cgaatgaaat tcggggaagt
     9601 ttgccgaagc gctcacgcaa cgccttctcg aattttccct gggcgcgaag ctgcgaaatg
     9661 aagtcggcca gctccggcgc acgccggtta aattcgctca gcaccgtagg cacggaccag
     9721 acaaacattc ccgcgttcca tcgaaaattt cctttccgca aaaacgattc agccagatcg
     9781 gggttgggct tttcgcgaaa acgcacgact cgatggatcg cgcttttatc gttcgatttt
     9841 cgcaaatgga ccggctcgcc atgctcgatg taaccgaaac cgggacaagc ccatgttggt
     9901 ctgatgccga tggttacaag ctcgcctgtc tcctcggctg catcgacagc cagggccaat
     9961 gtttcctgaa aagcagctcg gttcgcgatg acatgatcag cgggaagcac gatcatagtg
    10021 gccgcacgat cgcgcgccgc cacccaacca gttccgagcg cgaccgccgc ggccgtatcg
    10081 cgtttggctg gctccgcgag aatattttcc ttcgggaaac cgctcaccag tttgcgaacg
    10141 gccgcttcct gttcggcgtt cgtcagaatc agaatacgtt tatgcgaaac gaagccttcc
    10201 agtcgcgcga cggtttcttc gagaagcgtt ttgtcggaga ccaggcgaag gagttgcttc
    10261 ggccgggccc ggcgactcag cggccaaaaa cgttcaccac tcccgccggc gagaatgagc
    10321 acgtaagtcg gattgagcat tgaaacgagc gctgaccgaa ttttccaagc cgcgcgacga
    10381 ttttcccgtt tcttttttcg aaacgagacg tatatgatag gtcatgtctc tcccgatatc
    10441 catttcaaaa actgccgagc aactcccgat tgatccgttg ctggtcgtga cccgtgccgc
    10501 cattggcctc ggcgtggggt tattaatggc cgataaaatc aaaccttcga ttcgccaggt
    10561 agcggcgatt gcgctggtct caatcggtgc gctggccgcg gcgccctggt tggtcaaaat
    10621 cacattgggg caaatcaatc gaccggaatc ggagtggggg agtcgcgccc ggctgcgctc
    10681 cattcgggga gattctggtt acagctccga taacgatgtc tattgaggtt gttgtagagg
    10741 cgttcgccgt cgcgaaccct atttaaggac gcgacacacc cgaatccatc gcatccgccg
    10801 ggacctcaac ctggggtgtc aactcgttga ccgcgattct acctcggcgc gggacccgtt
    10861 gcagacccac gcgccgcgcc gcgagttcga caagttcggc tacccgtgac ttcagcacgc
    10921 gcgctcgcgt tgcgagaaag ccgagaaaca acaaataagg aatcgtgaac caaaaatcga
    10981 tatgaggaac gttcagctgc cgatacacca ggagactcac cgcaataagg ttgaagatga
    11041 cagtggtcgt tacgagacgg tcgcgaagcc ggacacgcgt caagcattta ccgccaccgt
    11101 gatattcagt gaccgtttgc agcgcgatac tccaccagaa atttccgtaa atttgaatgt
    11161 cccattcatt ccacccgcta tcggtcgaat aacgccagcc ttcctcatcg agcaattgga
    11221 agatcgcgcc cagtaggtag tgacgatccc tgccttcttc gctccagaaa acccgccggc
    11281 tcagactgcc tgcgacacgc accccattgg gcaaatgttc gtgcgctcga atcacgcttc
    11341 ggggcgtgcg cttgaaatgc agccacgtga aataccgcga ccaccctcgc acgagcggct
    11401 ggacgaaagc gagcaacatc acaagcaacc gcgcgtgcac cgtatcgaac ttcggctcga
    11461 tcggcgcccg caccatgtat gagagggcaa cgcacagcgt gccgccgatc atgagatagg
    11521 gcacgatccg caatgcaggc aacaaaattc cgaggccaaa gaggaaaacg gtaagggcga
    11581 accattcaat actgctgagg tacgccgcga cttcagattg tggcgtggga taaatcgatt
    11641 gaaaaaatcc ttcgccgaaa attccgtgat agatcactgg ccgattaatg aacgaaacag
    11701 gacggggcgc tccgtaaatc tggccacgcc atttggcagt gccagtcggg ccgaaaaaga
    11761 tcaaatgttt gaagcggagg agcgattccg cttcgccata gccctgctgc tgattcagaa
    11821 aagctcgcag cgtaaaacgc cgatggtgcc agacaatcgc ggtgggactg aacgcaatca
    11881 caaacccggc ctgttggagc cgccagcaaa aatcaacgtc atcgcccgct ttacgatatt
    11941 ccggatcgaa gccgcccatg ctctcaaacg cccaacgatg gaacgccatg ttacaaccag
    12001 gaatgtgctc ggcgaccgta tcggtgagca gaacatggct gggaccgccc ggcgccgctg
    12061 cgacgcaagc ctgaatccag ttttgtgccg gcggcgtaac gttcggtccg ccaactcccg
    12121 cgtagtcgcc gttgacaagg gtggccgtta aatagtagag ccaatcgaca tcggccatgc
    12181 agtcagaatc ggtataggca aacacttcac c
//
LOCUS       DASQUQ010000150         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_678166, whole genome shotgun sequence.
ACCESSION   DASQUQ010000150 DASQUQ010000000
VERSION     DASQUQ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678166"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VE486_04330"
     CDS             <1..267
                     /locus_tag="VE486_04330"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HZA38347.1"
                     /translation="GAQGGAELHWLPGEEMTWRAWREAHPDGKVLSTQTGHARDYSEN
                     AYTSYEHSGETMFPVKWSRQELSKKSWIVGTVINRQAKPMWLMI"
     gene            465..821
                     /locus_tag="VE486_04335"
     CDS             465..821
                     /locus_tag="VE486_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HZA38348.1"
                     /translation="MSKAAFIILAAGDTHESLGRVVNAFMGALEYIESGGEVQIIFDG
                     AGTQAAADFAKSDHKYHELFEKVRKRISGVCSYCAGAFKVKEKIEAANLPLSDQFNGH
                     PSFKQLIDQGFDVLTF"
     gene            complement(829..1347)
                     /locus_tag="VE486_04340"
     CDS             complement(829..1347)
                     /locus_tag="VE486_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZA38349.1"
                     /translation="MRKLWEEHIRHEFATHDTEETLATMVEDAYVNHIPVLTGGVGRE
                     QLREFYSKRFIPQMPPDTEMIPISRAIGVDQLVDEMIFKFTHTIRMDWMLPGLVPTGK
                     RVEVPLVAIVRFRDGKLAHEHIYWDQASVLVQLGLLDASKLPVAGVESARKALDKTQP
                     SNQLIDRADQAQ"
     gene            complement(1439..2158)
                     /locus_tag="VE486_04345"
     CDS             complement(1439..2158)
                     /locus_tag="VE486_04345"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HZA38350.1"
                     /translation="MKKTTWKWFANGRLVALGVIVIALFLAMKFLPVQRWLEDFNNWI
                     GQMGVAGIFIFIAVYAVATVLLAPGAILTIGAGFAFGLWKGFLAVSAGATLGAALAFL
                     VARFIARDKIETMAKGNEKFRNIDNAIGKQGAKLIFLLRLSPVIPFNLSNYFYGLTAV
                     KFWPYVLASWIGMMPGTFLYVYLGTAGKAAVAAAAGGEAVKHGWQYWTFLSVGLLATI
                     IVTIWVTKIAHGALKKTADVK"
     gene            complement(2155..>2697)
                     /locus_tag="VE486_04350"
     CDS             complement(2155..>2697)
                     /locus_tag="VE486_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38351.1"
                     /translation="HLEGRTAAFFCYGDGGGDELDEKGRPTILLHPEWFDPHEEPYPA
                     QRFYYGPLVWQCRYSGIQVPDELWTFVEFGHGKKYSDNQAEDMDSEPQVYREFEAWCM
                     RFAEHVARKGKIEPGRWRAFGYKAPGHFLRDAQLKWRDIRMRIGHPHEHSSPAKQQRL
                     GLNKDATLSPKTGEGEKLRE"
BASE COUNT          611 a          758 c          763 g          565 t
ORIGIN      
        1 ggcgcgcaag ggggcgcgga actgcattgg ttgccgggcg aagagatgac ttggcgcgcg
       61 tggcgcgaag cgcatcccga tggaaaagtt ctttccacgc aaaccggcca cgcccgcgat
      121 tacagcgaaa acgcatacac cagttacgag cactcgggcg aaacgatgtt tccggtgaaa
      181 tggagccggc aggagttgtc gaagaaaagt tggatcgtcg gaaccgtaat caaccgccag
      241 gcgaagccta tgtggttgat gatttagaaa agcaaacgcg cttgcaggac aacgtcgctg
      301 gccagcaaat tgaaataggt tacgacccgg cgaaacgcag cgctgtaatc accgacaaac
      361 acagtggccg acccattgca tttacaatgt cgtactggtt tgcgtggcag gcattttatc
      421 caaagacaga actattccgg cagtgagcgg cgaaagaaat ttgcatgagc aaagcagcat
      481 tcattatcct ggcagcgggc gacacgcacg agagtttggg tcgcgtggtc aacgcgttca
      541 tgggcgcgct tgaatacatc gagagcggcg gcgaagtgca gatcattttc gacggcgctg
      601 gcacgcaggc ggcggcagac ttcgccaagt ccgatcacaa ataccacgag ctattcgaaa
      661 aagtgcgcaa gcggattagc ggcgtttgta gctactgcgc cggcgcgttc aaagtgaaag
      721 agaagatcga agccgccaat ctgcccctca gcgatcagtt caacggccat ccgagtttta
      781 aacagctgat cgaccaggga ttcgatgtgc ttacgtttta agatcgaact actgggcttg
      841 gtctgcgcgg tcgatcaact gatttgacgg ctgagtcttg tcgagcgcct tgcgcgcact
      901 ctccacaccc gcgacgggca atttgctggc atcgagcaac ccgagctgca cgagcacgga
      961 ggcctggtcc cagtagatat gttcgtgtgc gagcttgcca tcgcggaacc ggacaatcgc
     1021 gacgagcgga acttccaccc gtttcccagt agggacgagc ccgggcaaca tccagtccat
     1081 cctgatggtg tgagtaaatt taaagatcat ttcatccacc agttgatcga cgccgatcgc
     1141 tcgtgagatg ggaatcatct ccgtgtccgg aggcatctgc gggataaacc gcttggagta
     1201 aaactcccgc aactgttccc ggccaacacc tccggtcagg actgggatgt gattgacgta
     1261 ggcatcctcc accatcgtgg cgagcgtctc ttcggtgtca tgtgtcgcaa actcgtgccg
     1321 aatgtgttcc tcccagagct tgcgcaagat ctcttgggcc gccgtgaggt ttgcttcgga
     1381 ttattgttga tcttgtttgc tcttaaataa cgctaacgtg cgtgccggcc aaatgcgatc
     1441 acttcacatc cgcagtcttc ttgagagcac catgcgcaat cttcgtgacc cagatggtca
     1501 cgatgattgt cgcgagaaga ccgacgctca gaaatgtcca gtactgccag ccgtgtttta
     1561 ccgcttcacc gccggcagcg gccgcaacag cagccttgcc agccgtgccg agataaacgt
     1621 agagaaacgt gccgggcatc atgccgatcc agctcgccag cacgtagggc cagaacttca
     1681 cagcggttag gccgtagaaa taattgctca gattgaacgg gatcacggga ctaagccgga
     1741 gtagaaagat taacttcgcg ccttgcttgc caatcgcgtt gtcaatgttg cgaaattttt
     1801 cgtttccttt cgccatcgtt tcgattttgt cgcgcgcgat gaaccgagcg acgaggaagg
     1861 cgagggccgc gccgagtgtc gccccagcgg aaacggcgag aaaacctttc cataaaccga
     1921 aggcaaatcc cgcgccgatg gtcaggatgg ctccgggcgc aagcaagacg gtagcgacgg
     1981 catacacggc gataaaaatg aaaatgccgg ccacgcccat ttggccgatc cagttgttga
     2041 aatcttcaag ccatcgctgg accggcagaa atttcatcgc gagaaagagc gcaatcacga
     2101 tcacgccgag agcgacgagc cgtccgtttg cgaaccattt ccaggtcgtc tttttcattc
     2161 ccgcagcttt tcgccctcgc cggtcttggg cgaaagcgtc gcgtctttgt tcaggccgag
     2221 cctttgctgc ttagccggcg atgagtgttc gtgcgggtgg ccgatgcgca tccggatgtc
     2281 gcgccacttg agctgcgcat cgcggaggaa atgccccgga gccttgtacc caaaagcccg
     2341 ccagcggccc ggctcaattt ttcctttccg tgctacgtgc tcggcaaacc gcatgcacca
     2401 ggcctcgaat tcgcgataga cctgcggctc gctgtccatg tcctcagcct ggttgtcgct
     2461 atattttttc ccgtggccaa attccacgaa cgtccacaac tcatcgggca cttgaatgcc
     2521 gctgtatcgg cactgccaga caagaggacc gtaatagaag cgctgcgccg gatacggttc
     2581 ttcatgcgga tcgaaccatt ccgggtgcag caaaattgtc gggcgtcctt tttcatcgag
     2641 ttcatcgccg ccgccgtcgc cgtagcagaa aaacgcggcg gtccggcctt cgagatg
//
LOCUS       DASQUQ010000151         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1198579, whole genome shotgun sequence.
ACCESSION   DASQUQ010000151 DASQUQ010000000
VERSION     DASQUQ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198579"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VE486_04355"
     CDS             complement(<1..916)
                     /locus_tag="VE486_04355"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZA38352.1"
                     /translation="MNDTAWQRLFGFHHAFDHPVTVVVALTAVVLLVLTPLLIFIITR
                     VGKSTAEKRKELWDRYRSWIWLALCILIPILAGAFWTILAVGTLSLVCYREYARITGL
                     FRERTISFIVVIGILLITFAELDNWYRLFVALFPLIVALIAIGGLIPDQPKGYIQRVG
                     LGVLGFALFGSALGNLGYMANDWNYRPMLLLIIFAVELNDIFAYICGHLFGHRKFVPN
                     TSPNKTVGGALGAIMLTTPLVAMIAHFIWKDTALDNSVCLIGLGIIVSVVGQFGDLML
                     SSIKRDLGLKDTAKFIPGHGGLLDRFDSL"
     gene            complement(913..1575)
                     /locus_tag="VE486_04360"
     CDS             complement(913..1575)
                     /locus_tag="VE486_04360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZA38353.1"
                     /translation="MNESAAKTIDRRPIATRNRKWAQAAAAWLASRNVSPNAISIAGM
                     CACIAAGLALGLTSFEYNRVFWLIAAVGAQLRLTANMLDGMVALVSGRDSKVGELYNE
                     VPDRVSDAAVFIGAGYAWGGNVALGYIAAILAIFTAYIRAAGKIAGAPNEFCGPMAKQ
                     HRMLVITVACLYSVIVPRSWQIFHFDDSDLGVMSFALALIIIGCIITVLRRFKRIASA
                     LR"
     gene            1707..2621
                     /locus_tag="VE486_04365"
     CDS             1707..2621
                     /locus_tag="VE486_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HZA38354.1"
                     /translation="MPATLEQTGPNVVTPRSVFILRFISTVALWSVALLIMFTGYEVL
                     FWILISLFGMIGLWEFYRMLDHKGLPNFKITGMICGAVMLAGSFYYFSKIGPAGSYDF
                     EVAVLLFFLLTVFGRQMFARLRNDEPLQTMAYTLFGLLYVLWLFNFITKIVYLTPRSS
                     SGQVTGQFYVLYLIAVTKFSDMGAYLTGSAIGRHLMVPHISGKKTWEGFFGAIAFALL
                     CSLALYKLMPGHLSVLNWTHATVLGLLLGFAAVLGDLAESIIKRSTDVKDSGNLLPGI
                     GGALDLLDSLLFTAPLLFFYMRLVIHVA"
     gene            2643..>3120
                     /locus_tag="VE486_04370"
     CDS             2643..>3120
                     /locus_tag="VE486_04370"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZA38355.1"
                     /translation="MNRKPVVILGATGSIGESALKVARDIPERMQIVGLAAKSNAQKL
                     AAAANEVRPESICLVDETQMDVLRRALDYEPRIFSGESGLLEIACLANADLVLVAIVG
                     TGGLRPALAAIKAGKDLAVASKEILVMAGEIVMREARDKSVHVLPVDSEHNAIFQCL"
BASE COUNT          737 a          820 c          869 g          694 t
ORIGIN      
        1 tcaggctgtc aaaacgatcc aacaaaccgc cgtgaccggg aatgaatttc gcggtgtctt
       61 taagaccgag atcgcgcttg atcgacgaga gcatcaaatc gccaaactgg ccgaccacgc
      121 tgacgatgat gccgaggccg atcaagcaga ccgaattatc caaagcggtg tccttccaga
      181 tgaaatgagc gatcatcgcc accagcggcg tggttaacat gatcgcgccg agcgccccgc
      241 caacggtttt atttggactc gtgttcggaa caaattttcg atgaccaaag agatgaccgc
      301 agatgtaggc aaagatgtca ttcagttcca cggcaaaaat gatcaacaac aacatcggcc
      361 gatagttcca atcgtttgcc atgtagccaa ggttaccgag cgcgcttccg aaaagagcaa
      421 atccgagaac accgagaccg acgcgttgga tgtatccttt cggttgatca gggattaacc
      481 cgccaatggc aatcaatgcg acgatcaaag gaaataacgc aacaaacagc cgataccagt
      541 tatccagttc ggcgaaagtg atcaggagaa tgccgataac cacgatgaaa ctgatggtgc
      601 gttcgcggaa gagaccggtg atgcgcgcat actcgcgata gcaaacaagg ctcagcgtgc
      661 ctacagccag gatcgtccaa aaagctccgg ccagaatcgg aatcagaatg cagagcgcca
      721 gccaaatcca ggagcgataa cggtcccaga gttcctttcg cttttcggca gtgctctttc
      781 ccactcgcgt tatgatgaaa atcaggagcg gtgtcaggac gagcagaaca acagctgtca
      841 gcgccacaac aaccgtcacg ggatgatcga aagcgtgatg gaatccgaat agccgctgcc
      901 aagccgtgtc gttcatctca aagcgcttgc gattcgtttg aacctgcgaa ggactgtaat
      961 gatgcagccg atgattatca gagcgagagc aaagctcatg acgccgagat cggaatcatc
     1021 gaaatgaaaa atctgccacg accgcggaac gatcacggaa tagaggcagg caacggtaat
     1081 gaccagcatc cggtgttgtt tggccattgg cccgcaaaac tcgttaggtg cgccggcaat
     1141 ttttcctgcg gcgcgaatgt aagcggtaaa gattgcgaga atcgctgcga tataacctag
     1201 tgcaacgttt ccgccccagg cgtagccggc gccgatgaac accgccgcat ccgaaacgcg
     1261 atcgggaact tcgttgtaaa gttcgcccac ttttgaatcg cgacctgaca cgagtgcgac
     1321 catgccgtcc agcatgttcg ccgttagccg cagttgcgcg ccaaccgcgg cgatgagcca
     1381 gaagactcga ttgtattcaa aggaagtcag gcctagtgcc aagccggctg cgatacaagc
     1441 gcacattccg gcaatggaaa ttgcattcgg cgaaacattt cgtgaagcca gccatgccgc
     1501 cgctgcctgt gcccattttc gatttcgcgt ggcaatgggc cggcgatcga ttgttttcgc
     1561 cgctgattcg ttcatcgcgg gcgcaatcca tagcaggatt gatgagcgat gaccagataa
     1621 caactcggga gagcgtcgcg cattagtcgc aacactgatg cgctccctct tgcatagcat
     1681 gccttccttc gggatgatgc agggccatgc ctgcgacgct ggaacaaacc ggaccgaacg
     1741 tagtgacgcc gcgttccgtg ttcattctgc gattcattag caccgtcgcc ttatggtcag
     1801 tcgccctctt gatcatgttc accggttacg aggtcctgtt ctggatattg atcagcttgt
     1861 tcggaatgat cgggctttgg gaattttatc ggatgctcga tcacaaaggc ctcccgaatt
     1921 tcaaaatcac cggaatgatt tgcggcgcgg tgatgcttgc cgggagtttt tattatttct
     1981 ccaaaatcgg accagcaggt tcctacgatt tcgaagtggc tgtcctgctt ttctttctac
     2041 tgacggtatt cggacggcaa atgtttgcac ggttgcggaa cgacgagcca ctgcagacga
     2101 tggcttacac attgttcggt ctgctttacg tgctttggct ttttaatttc ataaccaaaa
     2161 ttgtgtatct cactccgcgt tcgagcagcg gccaggtcac cggccagttc tacgtcttgt
     2221 atttgatcgc ggtcaccaag ttctcggaca tgggcgcgta tttgactggg agcgcgattg
     2281 gaaggcattt gatggtgccg cacatcagcg ggaagaaaac ctgggaaggt tttttcggcg
     2341 cgattgcgtt cgcgttactt tgcagcctcg cgctttacaa actcatgccg ggccatctat
     2401 cggtgctcaa ctggacacac gcgaccgtgc tcgggctgct gctggggttt gctgcagtcc
     2461 tgggcgattt ggctgaatcc attatcaagc gcagcaccga cgtaaaagac tcgggcaatc
     2521 tgctcccagg aattggcggc gcactcgatc tgctggacag cctgcttttc accgcgccgc
     2581 tattgttttt ctacatgcgg ctggtgattc acgtcgcgta gattcgagga ctcattaatc
     2641 aaatgaatcg taaacctgtc gttattcttg gcgcgactgg gtccatcggc gagagcgcgt
     2701 tgaaagtagc gcgcgatatt ccggagcgga tgcagatcgt cgggctcgcg gcgaaaagca
     2761 acgcacaaaa actggccgcc gctgcgaacg aagtccggcc ggaatcgatt tgtctggttg
     2821 atgagaccca gatggatgtt cttcggagag cactagacta cgagccgcgg attttctctg
     2881 gcgaatcggg tttgctcgag atcgcctgcc tagcaaacgc ggacttggtg ctggtggcaa
     2941 tcgtcggcac gggcggtttg cggcccgcgc tggcggcgat caaagcgggc aaggatcttg
     3001 ccgtggcgag taaggaaatt ttggtcatgg cgggcgagat cgtaatgcgc gaagcgcgcg
     3061 acaaaagcgt gcacgttttg cccgtggact cagagcacaa cgcgatcttt cagtgtctcg
//
LOCUS       DASQUQ010000152         4322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1040537, whole genome shotgun sequence.
ACCESSION   DASQUQ010000152 DASQUQ010000000
VERSION     DASQUQ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040537"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(562..2415)
                     /locus_tag="VE486_04375"
     CDS             complement(562..2415)
                     /locus_tag="VE486_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA38356.1"
                     /translation="MSTVWRVFAYLRRYPWMATGTLACAIISTVMIAAFPAVTKRIVD
                     EVLIEHHLEQLTPLALIAALAFLLQHGFNSLRIILSNTFEQKVIFDLRSDLYSHIQML
                     PLRWFDNRATGDLMTRILEDVSSVERMLIDGIEQGSVALLQLVIVLAMMFYVSVSLSL
                     VGLAPLPFLAGGALWYTLTAHRRYRLQRQAASGMNSLLHDNLAGIRQIKSFVREREEH
                     VRFNSVSDRLRHATLVVMKVWAIYHPSMYLIGSLGIVLTVWVGARHVLNGTMQPGDLF
                     AFLMLTGFLYEPVNKLHSLNQLVQAGRAAGERVFEIMDEPIEPAWDSAKTKVTRVIGD
                     VRFNDISFAYSKEGPPALQHITFHAQPGKTIALVGTTGAGKSTLVNLLTRFYEYESGE
                     ILIDGTPIRDFAMRRLREFIGVVTQESFLFNGTIRENLLMGKPAATDAEILVAAEAAN
                     ARVFIDRLPKGLDSVVGERGVKLSVGEKQRLSIARALLKDPPILILDEATASVDTATE
                     RLIQEALEHLMSHRTCFVIAHRLSTVLRADQILVLERGRIIERGTHATLLERGEKYAQ
                     LWEHSFLEKPAEELEAVAEPAIALTGRILFRACVSKLRKFALRRKPHQIMR"
     gene            2611..3177
                     /locus_tag="VE486_04380"
     CDS             2611..3177
                     /locus_tag="VE486_04380"
                     /inference="COORDINATES: protein motif:HMM:NF042424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1102 family pilus-like protein"
                     /protein_id="HZA38357.1"
                     /translation="MKAGFLLGLSIFLPLIVSAQTRAGAEFQITKITKNLIPTPQFAH
                     AGGAQYQTNQRDRWLEVEVEFKATPELTDELTFKYYILFNGTLLTGEVTHVNIPAGRE
                     NRSVMYIPPRALARFANNRAITENSCQNIAVQIVQQGAVKAETSLNRAQPQWFAALPQ
                     VSGFVLNKDQTPFAPLYWDRYEQIKTAR"
     gene            3390..>4322
                     /gene="pilM"
                     /locus_tag="VE486_04385"
     CDS             3390..>4322
                     /gene="pilM"
                     /locus_tag="VE486_04385"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HZA38358.1"
                     /translation="MPLPTRLISLNLGSQTIGLAEFRTQPHGGIVLVDYRLREIPGEP
                     VDEQTRHAQITVALREMMDELRIKHGRINYAIPAQSAFARFVKLPLVDEEKIERVIGF
                     EAQQNVPFPIDEVVWDYQIIGGGPDKQVEVVIVAVKAAYLEAINAAVEESGLRADKIE
                     VATMSLCNAFRYNYAGTPGCSLLVNIGFRTTNVLFIEPGKFFSRSVPIGGSSIVAAIA
                     KEFGESFAEAESRKRRDAFVSLGGAFAEPADPDVARVSKIVRATMTRLHAELMRSISH
                     YRAQQQGSRPDRIFLCGGSAGMPYMREFFHEKLQL"
BASE COUNT          972 a         1193 c         1214 g          943 t
ORIGIN      
        1 accggcgtta actcgcggga aatggcgaag cgcgcgtctc cgtgcgggta cgctcgagtc
       61 tgtgtttagc gccaagggcg cggcctcacg atagactggg gcatcgcgcc ccaggaatcc
      121 aaatcaccca taaccgagcg ctgaaagcgc gattcaaccc agtggcaatg ctccgaccta
      181 aaagatcgtg cgcgcgtcga aatcggcgcg aaagtgaatc gcgccttcag cgctgcattt
      241 tttagattta cggatcctgg ggcgttgccc cagtcttgca atatgaagcc gcgcctctga
      301 cgctaaacac gtaatcgctc gaagatcgaa tattgctcgg cccgctacga tgtgggaaat
      361 aaccgccgaa tgggtataat gttaacctca tatgaaagct ggcctcctgg ggctttccct
      421 tttccttccc ttgattgtct ctgcgcagac gcgcgccccg ggggccgagt ttcaaattac
      481 gaaaatcatg aagaatttga ttccgacgcc gcaattcgct catgctggcg ggtcgggacc
      541 ttcttcaagg aactaaaggg attatcgcat tatttgatgg ggcttccttc tgagtgcaaa
      601 tttgcgcaat ttacttacgc aggcgcggaa caatatccga ccagtcagcg cgatcgctgg
      661 ctctgccacc gcttccagtt cctctgcggg tttctccagg aaactgtgct cccataattg
      721 ggcgtatttt tcgccccgtt ccagcaacgt tgcgtgcgtg ccgcgctcga taatgcggcc
      781 gcgttccaac acgagaatct gatcggctct caggacggtg gacaggcgat gcgcgataac
      841 gaaacaggtc cgatgcgaca tcagatgttc gagcgcttcc tgaatcaacc gttccgtcgc
      901 cgtgtccacg ctggcagtcg cttcatccaa tatcagaatg ggcggatctt tcaggagcgc
      961 acgcgcgatc gagagccgtt gcttttcacc gacgcttagc ttcaccccgc gctcgcccac
     1021 gacgctatcc aaccccttcg gtaaccgatc gataaacacc cgcgcattcg ccgcctcggc
     1081 cgccaccagg atctccgcgt cggtcgcagc cggttttccc atcagcagat tttcccggat
     1141 cgtgccgttg aacaggaaac tttcctgggt caccaccccg atgaattccc ggagccgccg
     1201 catcgcgaaa tcacggatcg gcgtcccgtc gatcaggatt tcgcccgatt cgtattcgta
     1261 aaaccgcgtt agcaaattga ccagggtcga tttcccggcg ccggtggtcc cgaccaatgc
     1321 gatcgtcttg ccgggctgag cgtgaaaagt gatgtgctga agcgcgggcg gcccttcctt
     1381 actgtaagcg aaactgatat cgttgaaccg gacgtcgccg atcactcgcg tcactttggt
     1441 tttggccgaa tcccatgccg gctcgatcgg ttcatccata atttcaaaga cgcgttcgcc
     1501 ggcggcccgt ccggcctgca ccagctgatt gagcgaatgc agtttgttga ccggttcata
     1561 gagaaatccg gtcaacatca ggaacgcgaa cagatcgccc ggctgcatcg tgccgttgag
     1621 gacatggcgg gcgcccaccc atacggtgag gacaatcccg agggaaccga tcagatacat
     1681 ggaagggtga taaatggccc agaccttcat cacgaccagg gtcgcgtggc gaagccggtc
     1741 gctgaccgaa ttgaagcgaa cgtgttcttc ccgttcccgg acgaagcttt tgatttgccg
     1801 gatcccggcc aggttatcgt gcaggagcga attcatgccc gaagcggcct ggcgttgcag
     1861 gcgataacgc cggtgtgcgg tcagcgtgta ccaaagcgcc ccgccggcta ggaacggcaa
     1921 cggggccagc ccgaccaggg aaagagaaac gctgacgtaa aacatcatgg ccaggacaat
     1981 aaccagctga agcagggcga ccgagccctg ctcgattccg tcgatcaaca tgcgttcgac
     2041 cgagctgaca tcctcgagga tccgggtcat cagatcgccg gtggcgcgat tatcgaacca
     2101 gcggagcggc aacatctgga tgtgggagta aagatcgctc cggagatcga agatcacttt
     2161 ctgctcgaag gtgttactaa gaatgattcg gagtgaattg aatccgtgct gcaagaggaa
     2221 cgccagggcg gcgatgagcg ccagcggggt gagctgttcc aagtgatgct cgatcaggac
     2281 ctcatcgacg atccgtttag tcacggccgg gaaagcggcg atcatgaccg tgctgataat
     2341 ggcgcaggcg agggtgccgg tggccatcca cggataacgc ctcaaataag caaaaacgcg
     2401 ccagactgtg ctcatgggaa ttggacgctg attatacgac gatatttgcg taaacgcctg
     2461 tggaaacgat gcgacgcgca ccgagcgccg ctgcaaataa aggcatatag aattcttgcg
     2521 tttcggttag aatcgaatcg taaaatcatg aaatgcttaa agcgtctcga ctaagtctgc
     2581 cgattcattg caagatcgac cggtaaccat atgaaagctg gctttctcct gggtctttcc
     2641 atttttcttc ctctgattgt ctctgcgcag acgcgcgccg gggccgagtt ccagattacg
     2701 aaaatcacga agaatttgat tccgaccccg caatttgctc atgctggcgg ggcacaatat
     2761 caaaccaacc agcgcgatcg ttggctggaa gtggaggttg aatttaaggc gacgccggaa
     2821 ttaaccgatg aattgacgtt taagtattac atcctcttta atggaacact gctgaccggc
     2881 gaagtgactc atgtaaatat tccggcgggc cgcgagaacc gctcggtcat gtacataccg
     2941 cctcgtgcac ttgcgcgctt tgccaataac cgggcgatca cggagaactc atgccagaac
     3001 attgcggtcc agattgtgca acagggcgcg gtgaaagcgg aaacgagcct caaccgagcg
     3061 cagccacaat ggttcgcggc tctgcctcag gtttccggat ttgttctgaa caaggatcag
     3121 acgccatttg caccgctcta ttgggatcgc tacgagcaga tcaagaccgc gcgctagcgc
     3181 ggagtttcaa aaagtcctga accacggatt gcacgggtaa cgcggatgaa aaaatgattc
     3241 acaaagagtc aagcggcaag ataatcggcg ctgcaaaaga atacgaccat cctgatcttc
     3301 atgcctgagt ttcctatctg tgctatccgt gtaatccgtg gtttctgatg ttttattcgt
     3361 gttcgtttag gatttggagc ttctagtcta tgcctcttcc cacgcgcctg atcagtctca
     3421 atcttggatc gcagacaatt ggtctggccg aatttagaac tcaaccccac ggcggcattg
     3481 tgctggtgga ctatcggctg cgcgaaattc ccggtgagcc ggtggatgag cagacgcgtc
     3541 acgcacagat cacggttgcc ttgcgcgaaa tgatggacga gctgcgcatc aagcacggcc
     3601 gcatcaatta cgcgattcct gcgcagtcgg ccttcgcgcg ttttgtcaaa ttgcccctgg
     3661 tggacgagga aaaaatcgaa cgggttatcg gattcgaggc gcagcaaaat gtgccattcc
     3721 cgattgacga agtcgtctgg gattaccaga tcatcggtgg cggacccgac aagcaggttg
     3781 aagtcgtgat tgttgcggtc aaggccgcgt atctcgaagc catcaatgcc gccgtggagg
     3841 aatccggatt gcgcgctgac aaaatcgaag tcgccacgat gagcttgtgc aatgcgttca
     3901 gatataacta cgccggcacc ccgggctgtt ctttgctggt caacatcggc ttccggacga
     3961 cgaatgtgct ttttatcgag cccgggaaat ttttttcgcg gagcgttccc attggtggca
     4021 gctcgattgt ggccgcgatc gccaaggaat ttggcgaatc tttcgcggag gctgaatctc
     4081 gcaagagacg ggatgccttt gtcagtctgg gaggcgcgtt tgccgagccg gcagacccgg
     4141 atgtggctcg cgtctcaaaa atcgtgcgcg ctaccatgac ccggctgcac gcagagctga
     4201 tgcgttccat cagtcattac cgtgcgcaac agcagggaag caggccagac cgcatcttcc
     4261 tttgtggagg aagtgccggc atgccgtaca tgcgcgagtt ttttcacgag aaactccagt
     4321 tg
//
LOCUS       DASQUQ010000153         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_500417, whole genome shotgun sequence.
ACCESSION   DASQUQ010000153 DASQUQ010000000
VERSION     DASQUQ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500417"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VE486_04390"
     CDS             complement(<1..633)
                     /locus_tag="VE486_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZA38359.1"
                     /translation="MNNLTISPNIMEISERAAQLTPSLTLSIDSKAKAMKAEGIDVCG
                     FGAGEPDFDTPEHIKKAAIEALEAGFTKYTPSAGIPELRQAIAEKLAADNDLNYRAAQ
                     VIVSNGAKHACYNAILATCQPGDEVIIPAPYWVSYPDMVRLAGADPVIVPTSERNSWK
                     FRASDFENAMTPRTKMLILNTPGNPTGSVYTREELQAIVDVAAEEDIYILS"
     gene            complement(648..1724)
                     /locus_tag="VE486_04395"
     CDS             complement(648..1724)
                     /locus_tag="VE486_04395"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - obsolete nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZA38360.1"
                     /translation="MQTEELSYAIITPYSMRKSRTGGIVGRLISRTGLDLVGGRMFAP
                     SQELAQRYAETIVTETDARHRATQELIRDYVLKNFTGTVNGQRPRLLFLIFRGPDAVE
                     KIHRTVGHIVHERTSGETIRDTYGDYITDASGKVTYFEPGVLTSFDPKAVERDLNLWA
                     EFSDSDGGILDQVITFPADAKIEKTLVLIKPDNFKFPNLRPGGVIEVFSRSGLYIIGF
                     KVHRMSVAQAEEFYGPVLPVLEKKLGPKSGRENWESIVEFMAGKKPGECTPEERDKPG
                     SEKSIAIVYQGVDAVSKIRDVLGPTDPAKAPPGSIRREFGQTIMINAAHASDSAENAK
                     REMAIVRADENNFKPLIENFYRRQ"
     gene            complement(1925..>2339)
                     /locus_tag="VE486_04400"
     CDS             complement(1925..>2339)
                     /locus_tag="VE486_04400"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZA38361.1"
                     /translation="KRGWSLVDLGTGSGILALAASLFGAGSVIAIDADPLAISTAKAN
                     ARLNKIDNIHFQLGDLRQWKPAGTIDIVTANLFSELLIEILPKLKRGNWLILSGVLRA
                     QGQDFIRALRRNRIDIVEVRYRGKWIAVLARRSGT"
BASE COUNT          495 a          651 c          634 g          559 t
ORIGIN      
        1 cgacaaaata taaatgtcct cctcagcggc gacatcgaca atcgcctgca attcctcgcg
       61 agtgtaaacg gaaccggtcg gattacccgg cgtgttgagg atcaacatct ttgtgcgcgg
      121 cgtcatcgcg ttttcaaaat ctgacgcgcg aaatttccag gaattacgct cgctggttgg
      181 cacgatcacc gggtcggcgc cggctagtcg gaccatgtcg ggataactga cccaataagg
      241 cgccggaatg atgacctcgt cacccggctg acacgttgcg agaatggcat tgtaacaggc
      301 gtgtttcgcg ccattgctta cgatcacctg ggcagcccga tagttgaggt cgttatctgc
      361 ggctaacttt tccgcgatcg cctggcgcaa ctccgggatg ccggcgctgg gcgtatattt
      421 cgtgaagccg gcctccaatg cttcgatcgc cgcctttttg atgtgttcgg gcgtatcgaa
      481 atctggttcg cctgcgccga agccgcaaac atcaatccct tcggctttca tggctttggc
      541 cttgctgtcg atcgagagtg tgagcgaagg ggtaagttga gcggcgcgtt cggaaatttc
      601 catgatattc ggactaattg tgaggttgtt caaaatggga gcgcaagcta ttggcggcgg
      661 tagaagtttt caatcaaagg tttgaagtta ttctcgtccg cccggacgat cgccatttcg
      721 cgtttggcgt tctcggcaga atcggaagcg tgcgcggcat tgatcatgat ggtctgacca
      781 aattctcttc ggatcgagcc gggcggcgcc ttcgctggat cggttgggcc aaggacgtcg
      841 cgaattttgc tcactgcatc cacgccttga taaacgatgg cgatggattt ttcgctgccc
      901 ggcttgtcgc gttcttccgg cgtacattcg cctggcttct ttcccgccat gaattccacg
      961 atgctttccc aattctcgcg cccgcttttt ggaccgagct tcttctcgag aacgggcagc
     1021 accggtccgt agaattcctc cgcttgcgcg acactcatcc gatggacctt gaaaccaatg
     1081 atgtagagcc cggagcggga aaaaacctcg ataacgccgc ccggtcgcaa gtttgggaac
     1141 ttgaaattat ccggcttaat caagacgagc gtcttctcga tttttgcgtc agcgggaaaa
     1201 gtgatgacct gatcgaggat tcctccatct gaatcggaaa attccgccca caagttcaaa
     1261 tcacgctcga cggcttttgg atcgaaggac gtgagcaccc caggctcaaa gtaagttacc
     1321 ttacctgaag catcggtgat gtaatcaccg taggtatcgc gaattgtctc gccgctggtg
     1381 cgttcgtgca cgatgtggcc aaccgtgcga tgaatctttt ccaccgcatc cggcccacga
     1441 aagatcagga acagcaggcg aggtcgctga ccgttaacgg tgcctgtaaa atttttcaga
     1501 acatagtccc gaatcagttc ctgcgtcgcc cgatggcgcg catccgtctc cgtgacaatc
     1561 gtttctgcgt agcgttgtgc caattcctga ctcggtgcaa acatgcgtcc gccgaccaga
     1621 tcaagtccgg tgcgcgaaat caggcggcca acgatgccgc ccgtgcgcga tttgcgcatc
     1681 gaatatggag taatgatcgc gtaggaaagt tcttcagtct gcattcctga tcactgtagc
     1741 ggcggtctat gaccgtcgca aaattcttct aggaaggtac gtgcacagcc atcttggctg
     1801 tgggatgacg ggcatcttgc ctgtcgattt acactggcag gtagggatgc ctgcccgccc
     1861 cacaggctcg gaagcctgtg ctccggttct cttttgaaaa cttttcaaca gagctactga
     1921 aagtctatgt tcccgagcgt cgcgccagaa ccgcaatcca tttcccacga tatcgcactt
     1981 ccacgatgtc gatcctatta cgccgcagtg ctcgaatgaa atcttgcccc tgggcgcgca
     2041 agacgccgga gagaatcagc cagttgcctc gcttcagttt cggcaggatc tcgatcagca
     2101 attcgctgaa cagattagcc gtgacgatgt cgatcgtgcc cgccggcttc cactgacgca
     2161 ggtcgcccag ctgaaaatga atattgtcga tcttattgag acgcgcgttt gctttggctg
     2221 ttgaaattgc aagcggatcg gcatcaatgg caatgacact ccctgcgccg aaaaggctgg
     2281 ccgccagagc gagaatcccg ctcccggtcc caagatcgac aagcgaccaa ccgcgcttg
//
LOCUS       DASQUQ010000154         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_759446, whole genome shotgun sequence.
ACCESSION   DASQUQ010000154 DASQUQ010000000
VERSION     DASQUQ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759446"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VE486_04405"
     CDS             complement(<1..549)
                     /locus_tag="VE486_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZA38362.1"
                     /translation="MKKFSATQKATAIRKSTFALTLVFVAVAQTSALTPGDLSRVTFE
                     QHPGQQLSHDLVFRDENDRLFRLGNQFGKQPTILVLGYYRCPMLCTLINDGLIRALQE
                     LPLSVGRDFQVVDVSIDPTEKAAAAAAKKSEYLRQYGRSGAGDGWHFLVGNKPSITKL
                     ADTVGFRYAYDPEIQQYAHPSGI"
     gene            complement(546..1007)
                     /locus_tag="VE486_04410"
     CDS             complement(546..1007)
                     /locus_tag="VE486_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38363.1"
                     /translation="MDNSSAENERHGHERKDVDVFSIFSIALLLFLSCVIIFIVVAGI
                     MHYFRGHEPAKTAGQANIPITRTTEFPKPRLEVRASADLTKLRAAEEADLNSYGWVDQ
                     RQGTVRVPIDRAMQLILERGLPDVGVGQSPLSLMQARPAETATPPRLMQDQ"
     gene            complement(1016..2176)
                     /locus_tag="VE486_04415"
     CDS             complement(1016..2176)
                     /locus_tag="VE486_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38364.1"
                     /translation="MSIVNSSVTRAKSLLLIIGVIGLAGCAIGWITTPREFFIAYLFG
                     HFFFLGLSLGSLGLLMIHHLTAGRWGYAVRRFLESAVGNLPMLALLFTPIFLGLPYLY
                     PWMDPSVVAASDALRQKQFYLNTPGFVIRTAIIFAIWIVIARLLLKWSAEQDATVSVE
                     PTRKMRKLSGPGLVIYPVTMTFAAVDWLMSMEADWYSTMFPVLLCIGQILSALALVIL
                     LLAWVARSSPLSPLASEDTFHKLGNLLLAFTMMWAYLAFGQLLVIWSGNLPQEISWYL
                     HRVSDGWRWIAVSIAVFHFFVPFILLLMRPIKKRRCVLASVAACLFIAHIVAIWWTIA
                     PSIYGKQFYVSWIALAAFLGIGGIWSAAFLRNLERRPLVPLNDPRLALAVPA"
     gene            complement(2176..3039)
                     /locus_tag="VE486_04420"
     CDS             complement(2176..3039)
                     /locus_tag="VE486_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZA38365.1"
                     /translation="MPDHLHALISFPYERPMTQIIADWKRLLATQLKIEWQRDFFDHR
                     LRKEESYREKADYIRANPVRAGLITQSDDWPYFWTANSQKDANSEDRALCGGAPGGRA
                     LPVLLAIALLFTACRPDMANQPKAKPFSESDFFSNGTNARQLPPHTVAHGAAHENTTF
                     YTGLLNGVYVTKLPTKLTPELLARGRERFDAICASCHDRTGSGNGMVVQRGFPQPPSF
                     HVDRLRNAPIGHFFDVITKGYGVMYSYATRVDPEDRWAIAAYIRTLQLSHNARLSDVD
                     PDERAKLEGGR"
     gene            complement(3279..>3862)
                     /locus_tag="VE486_04425"
     CDS             complement(3279..>3862)
                     /locus_tag="VE486_04425"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HZA38366.1"
                     /translation="RPAVAPYQTAIVRMQFMNGRSTYGLLAEFSTHEELVRAAEKAYT
                     AGFRRMDGFAPFPVEGLAEALGKRNRLPLLVLIGGIIGGAGAYYMQWYANAIDYVINV
                     GGRPIHSWPSFIPITFELTVLAAALTAFFGSLALNGLPRPYHPVFHIPEFERASQDRF
                     FLCIEARDQNFDPDRTRAFLQSLNPLSIAEVPK"
BASE COUNT          942 a         1071 c         1064 g          785 t
ORIGIN      
        1 gattccgctt ggatgcgcat actgttgtat ctctgggtca taggcgtagc gaaacccgac
       61 tgtatcggcc agttttgtga tcgagggctt gttgccgacg agaaaatgcc atccatcccc
      121 ggcgccggat cggccatatt gccgcaagta ctctgatttt ttggccgccg cagcggcggc
      181 cttttccgtc ggatcaatgc tcacatcgac cacctgaaaa tctcgaccga cgctcaacgg
      241 cagctcttgt agtgctcgaa tcaggccatc gttaatcaac gtgcagagca tcggacagcg
      301 gtagtaaccg agcaccagaa tggtcggctg ctttccgaac tgattgccca gacgaaacag
      361 gcgatcgttt tcatcgcgaa aaacgaggtc gtgcgaaagc tgctggccgg gatgttgttc
      421 gaaagtcacg cgactaagat cgccgggcgt gagcgcgctc gtctgcgcaa ccgcgacgaa
      481 cacaagcgtt agcgcgaatg tcgatttgcg aattgcggtg gccttctggg tggcgctgaa
      541 tttcttcatt ggtcttgcat tagccgtggc ggtgtggcgg tttcggccgg tcgggcttgc
      601 atcaggctga gaggactctg tccgactcca acatcgggca aaccgcgttc cagaattagt
      661 tgcatggcgc gatcgattgg cacacgcacc gttccctggc gttgatcgac ccagccatag
      721 gagttgagat cagcttcttc ggcagcgcga agcttcgtca aatcggctga cgccctgact
      781 tccaatcgcg gtttgggaaa ttccgtagtc cttgttatcg gaatattcgc ctggccggcc
      841 gttttagccg gctcgtgccc tcggaaataa tgcatgattc ctgcgacgac gatgaaaatg
      901 atgacgcacg acaaaaacaa aaggagcgcg atcgaaaata tcgaaaagac atctacgtct
      961 ttccgctcat gtccatgtcg ttcgttctcc gcggatgagt tgtccatttc agcgctcatg
     1021 caggcacagc gagcgccaac cgcggatcat tcaacggcac aagcggtctc ctttccaaat
     1081 ttctcaagaa cgctgccgac cagataccgc cgattccaag gaatgcggca agagcaatcc
     1141 aactcacata aaattgtttg ccataaatac ttggcgcgat cgtccaccag atcgcgacga
     1201 tgtgtgcaat gaacaaacac gctgcgacgc tcgccagaac gcagcggcgc tttttgattg
     1261 gccgcatcag caaaagaata aacgggacaa aaaagtgaaa gacggcgatg gagactgcga
     1321 tccatcgcca gccgtcgcta acccgatgca gataccagga gatttcctgc ggcagatttc
     1381 cggaccaaat aacgagcaac tgcccgaatg cgaggtatgc ccacatcatc gtgaaagcca
     1441 gcagcaaatt gccaagtttg tgaaaggtgt cctcactggc aagcggactg agaggtgatg
     1501 agcgggccac ccaggcaagc agcaaaatca ccagcgccag cgcactcaga atctgtccaa
     1561 tgcacagaag taccggaaac atggtcgagt accaatccgc ttccatcgac atcagccagt
     1621 caaccgccgc aaatgtcatc gttactggat aaatcaccag acccggtccg ctcagcttcc
     1681 gcattttacg ggtcggctcc actgaaacag tggcgtcctg ctcggccgac catttcagca
     1741 acaatcgagc aatgacgatc caaatggcaa aaataatcgc ggtgcgaatc acaaaacccg
     1801 gcgtgttcaa ataaaattgt ttttgacgaa gcgcgtcgct ggccgccacg acggacgggt
     1861 ccatccacgg gtaaagatac ggcaggccca agaagatcgg cgtgaacaaa agcgccagca
     1921 tcggcaaatt gccaacggcc gattccagaa aacgccgtac cgcgtagccc caacgcccgg
     1981 cggtcagatg atggatcatc agcagtccga gcgatcctag tgaaagcccg aggaagaaga
     2041 agtgaccgaa aagatacgcg atgaaaaatt cgcgcggcgt cgtgatccag ccaatcgcgc
     2101 atccggccaa gccgattacg ccgatgatca acaaaagcga tttcgcgcga gtgacggaag
     2161 agttgacaat cgacatcatc ttccgccctc cagtttcgcc cgttcgtcag ggtcgacgtc
     2221 ggacaacctc gcgttgtggc tgagctgcaa agtgcggata taagcggcga ttgcccagcg
     2281 gtcctccgga tctacacgcg tcgcgtagga atacatcaca ccgtagccct ttgtgatcac
     2341 atcaaagaaa tgaccgatgg gcgcattacg caaacgatcg acatgaaatg agggcggctg
     2401 cggaaaaccc cgttgcacaa ccatgccgtt tccgctgccc gtgcggtcgt gacatgaggc
     2461 gcaaatcgca tcgaagcgct cgcggccccg ggcgagcagt tccggtgtca gcttcgtagg
     2521 cagcttagta acgtaaacgc cattcaataa gccggtgtag aacgtcgtat tctcatgtgc
     2581 cgcgccgtga gccacggtat gaggcgggag ttgtcgcgcg ttcgttccat ttgagaaaaa
     2641 atcgctttcc gaaaacggtt tggccttcgg ttgattcgcc atatccgggc gacaagcagt
     2701 gaatagcaac gcaatggcga gcaacactgg tagggcgcga ccgccgggcg cgccgccaca
     2761 caacgctcga tcttcactgt tcgcgtcttt ctgggaattg gctgtccaaa agtacggcca
     2821 atcgtcggat tgagtgatca atcccgcgcg aaccggattg gcgcggatat agtccgcttt
     2881 ttccctgtaa ctttcttcct ttcgtaaacg atgatcgaaa aaatcgcgtt gccactcgat
     2941 cttgagctgc gtggccagaa ggcgtttcca atcagcgatg atttgcgtca tcgggcgctc
     3001 atacggaaac gagatgagcg cgtgtaaatg gtccggcatt aagcagacca agtgtgcgtg
     3061 ccaaaaagca ttttgattca aaaattggac agaatcgaaa atggcgcgag ctattttcgg
     3121 atagcaaagt tggtttttgc cgcgcggcat gcaacaaact gtgacgaaga aaatctcgtc
     3181 ttcaggacgt aaccacagcg gccggtcgtg cgggagcttt ttgcgttgtg gacgattcgg
     3241 cgcgcccggc ggtcgcgccc taccaatttc acctggcttc atttcggcac ctccgcgatc
     3301 gacaacgggt tgaggctttg caggaaggcg cgcgttcggt cggggtcgaa attttggtcg
     3361 cgtgcctcga tgcagagaaa aaatcggtcc tgtgaggcgc gttcgaattc cggaatgtga
     3421 aaaacgggat gatacgggcg cggcaggccg ttcaaggcaa gtgagccaaa gaacgccgtg
     3481 agtgctgctg ccagcacagt cagctcaaac gtgattggaa taaacgaagg ccaactgtgg
     3541 atcgggcggc cgccaacgtt aatcacataa tcgatggcgt tcgcgtacca ctgcatgtaa
     3601 tacgcgccag cgccgccgat gattccgccg atcaagacca acaggggcag gcgatttctc
     3661 ttgccgagcg cttccgccaa gccttccacc ggaaacggcg cgaaaccgtc catcctacga
     3721 aaaccggccg tgtaggcctt ttccgctgca cgaactaact cctcgtgcgt cgaaaattcg
     3781 gcaagcaaac cgtaggtcga tctgccgttc ataaattgca tacgcacaat cgcagtctgg
     3841 tagggcgcga ccgccgggcg cg
//
LOCUS       DASQUQ010000155         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_308609, whole genome shotgun sequence.
ACCESSION   DASQUQ010000155 DASQUQ010000000
VERSION     DASQUQ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308609"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VE486_04430"
     CDS             <1..1190
                     /locus_tag="VE486_04430"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HZA38367.1"
                     /translation="LTVKREEEDKIALTQERFTVNFKNGPPLEWKIPLTYAVVGEAPG
                     TSLMTSKTDSLRNIPADRALKLNVNGAGNYRVQYDDLSWNLLLQALPNLGVADRVNLL
                     SDAWALVQANRAPVSLYFGLVEKLPASTDLAEREQIINVLDFINRLLIGSAERKQFQR
                     YARSLLRPTFETLGWEAKAGEPSSAGNLRASLIRALGDLNDSAIIAGCRERFKKYLTD
                     PQTLPPDLRPPVLAVVGHYADDETWNQLHELGLKTTSIEEKQNYYNALAGATEAKLVK
                     KTLPIALTNELPTSRAIFLVSRVARQSDHPELAWDFAKANMKTLLAKTDALGANRYAP
                     GLFTFFAENSRADELRAYAKANLPETSAPEVAKAADEIQFRSEFKRRLTPQLEAWIDK
                     KNR"
     gene            complement(1214..1690)
                     /locus_tag="VE486_04435"
     CDS             complement(1214..1690)
                     /locus_tag="VE486_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZA38368.1"
                     /translation="MSENIVIEPATEADLDELSELLGELFAQESDFRPDKGKQLRGLR
                     LIFEQPSRGRVFVLRREGAIVGMINLLFTISTAEGGFVILLEDLVIHKEYQGKGYGSK
                     LLQHAIDFAKQKNFVRITLLTDRPENVAQEFFRRHGFVESSMIPMRLWINPQNSDQ"
     gene            1838..2011
                     /gene="rpmH"
                     /locus_tag="VE486_04440"
     CDS             1838..2011
                     /gene="rpmH"
                     /locus_tag="VE486_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZA38369.1"
                     /translation="MKRTYQASKRTRKRQHGFRARMKTKGGRAILARRRQRGRKRLLP
                     KGVEKHYARHTRA"
     gene            complement(2039..2779)
                     /locus_tag="VE486_04445"
     CDS             complement(2039..2779)
                     /locus_tag="VE486_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZA38370.1"
                     /translation="MLTTALLGIWRALAVGFFSLCALVENAITIPFFAKADRLRARTA
                     WLRRWSRFACRVVGIRVTTRGSMPRSGLLVCNHLSYLDVIVLSSIRPCLFVAKRDVAT
                     WPLFGWLARVAGTIFVDRERRLASADVVDLVRDAITDGSLVVLFPEGTSSDGSTVLPF
                     KSALLESAVQLRCPVAAAAIEYAIDDGSVADEVCYWGDMTLAPHLLNLFFKRKIRATC
                     LFSVPRIRSGDRKEIAHELREEIVSMRS"
     gene            3007..3079
                     /locus_tag="VE486_04450"
     tRNA            3007..3079
                     /locus_tag="VE486_04450"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3040..3042,aa:Lys,seq:ctt)
     gene            3266..>3583
                     /locus_tag="VE486_04455"
     CDS             3266..>3583
                     /locus_tag="VE486_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZA38371.1"
                     /translation="MAQKIELHVNGRAHNVEVEPETPLLYVLRNYLELNWPRYGCGYE
                     QCGACMALVNGVPLPTCKMPVASAVGKTIRTLEDLGTEEKPHPLQRAFIQEQAAQCGY
                     CISG"
BASE COUNT          854 a         1011 c          982 g          736 t
ORIGIN      
        1 tcctaacggt caagcgagaa gaagaggaca agattgcgct tacccaggag cgcttcacgg
       61 tcaatttcaa aaacggtccg ccactggaat ggaaaatccc gctaacttat gcagtcgttg
      121 gagaagcgcc cgggacttcg ctgatgacca gcaaaaccga cagtttgcga aacatccccg
      181 cagatcgagc gctcaagttg aatgtaaatg gggctggaaa ttatcgcgtc caatatgacg
      241 atctttcgtg gaatctattg ctccaggctc tgccgaattt gggcgttgca gatcgcgtta
      301 acctcctgag cgacgcgtgg gcgctggtcc aggcaaatcg cgcgccggtc tcgctctatt
      361 tcggattagt ggaaaaattg ccagcttcga ccgacctcgc cgagcgcgaa cagattatca
      421 acgtgctgga ttttattaat cggctgctca tcggtagtgc ggaacggaaa caatttcagc
      481 gttacgcgcg atcactcttg cggcctacgt ttgagacgct cggctgggaa gcgaaggctg
      541 gcgaaccgtc ttctgccgga aatttgcgtg caagcttgat ccgcgcactt ggggacctaa
      601 acgattcggc gatcatcgcg ggctgtcgcg aacgttttaa gaaatatctc accgatccgc
      661 agacgctccc gcctgatttg cgtccgccgg ttctggctgt ggtcgggcat tacgccgacg
      721 acgagacctg gaaccaactt cacgaacttg gtctgaaaac aacgagcatt gaggagaaac
      781 aaaattatta caacgcgctg gctggcgcga ctgaggcgaa gttggtgaag aaaacgctgc
      841 ccattgcgct caccaatgag ctgccaacca gtcgtgcgat atttcttgta tcgcgagtcg
      901 cgcgccaaag cgaccacccg gaactcgcgt gggattttgc gaaggcgaac atgaaaacat
      961 tgctcgccaa gaccgatgcc ctcggcgcga accgatacgc ccctggtctg ttcactttct
     1021 tcgcggaaaa ttcgcgcgcc gatgagttaa gagcgtatgc gaaagcaaac ttgcctgaga
     1081 ctagcgcgcc ggaagtcgca aaggcggccg atgaaattca gtttcggtcc gagttcaaaa
     1141 gacgtctcac gccgcagttg gaagcttgga tcgataagaa aaaccgctga taacagagat
     1201 cactgatcac cgatcactgg tcactattct gagggttgat ccacaagcgc atcggaatca
     1261 tggatgattc gacgaagccg tggcgacgga agaattcctg cgccacgttt tctggccggt
     1321 cggtcaaaag agtaatgcgc acaaaatttt tctgcttcgc gaagtcgatt gcgtgttgga
     1381 ggagcttcga accgtagcct tttccctggt attctttgtg gatgaccaga tcctcgagca
     1441 ggatgacgaa accgccttca gccgtgctga tggtgaagag caaattgatc atcccgacga
     1501 tggcgccctc gcgtcggagc acgaaaacgc ggccccggct gggttgttcg aaaatcagac
     1561 gaagcccgcg gagctgtttc cctttgtccg ggcgaaaatc gctctcctgg gcaaataact
     1621 cgcccagcag ctcggacaat tcgtcgaggt cggcctctgt cgcaggctcg attacgatat
     1681 tttcgctcat ttgttcgcac cgcttatttc aaaaatcggg ggcactggca atccccgaat
     1741 gaaaggtcgg caagcgcata gcacagctcg attgacaatg catccctggc ccctactgta
     1801 accgctctta aatcgcgccg aaattatctt cggttttatg aaacgaactt atcaggcatc
     1861 caagcgaaca cgtaaacgcc agcacggttt tcgtgcccgc atgaagacca aaggcggtcg
     1921 agccattctt gcgcgccggc gacaacgcgg ccgcaagcgc ttgctgccaa aaggcgtcga
     1981 gaaacattac gcgcgccata cgcgagcgta acatcacgtg cgaaggcgcg atccctcgtc
     2041 atgatcgcat cgatacgatt tcctcgcgta gctcgtgtgc gatttccttg cgatcgccgc
     2101 tccgaattct tggaacagaa aacaagcacg tcgcccggat tttgcgtttg aaaaaaagat
     2161 taagcaaatg cggcgccagg gtcatgtccc cccagtagca tacctcatcg gcgactgaac
     2221 cgtcatcgat cgcgtattcg atagcggcag ccgcaaccgg acaacgaagt tggacggctg
     2281 attccagcag ggccgacttg aatggcaata ccgtcgagcc gtctgaactt gtcccttcag
     2341 gaaacaggac cacaagcgac ccgtcagtaa tcgcatcgcg cacaaggtcg acaacatccg
     2401 cactcgccaa tctgcgttca cgatcgacaa aaattgtgcc agcgacgcga gccagccatc
     2461 caaatagcgg ccatgttgct acgtcgcgct tggcgacaaa gaggcacggc cgaatcgagc
     2521 tgagcacaat cacgtcgaga tagctaaggt gattgcacac caaaagtccg gagcgcggca
     2581 tcgaaccccg agtcgttacg cgaataccga caacgcgaca cgcaaaacgg ctccagcgac
     2641 gcagccacgc agtgcgtgcg cgcaggcgat ccgccttggc gaagaacgga atcgtgatcg
     2701 cattctcaac gagagcgcag agcgagaaaa accccacggc caacgcgcgc cagattccaa
     2761 gaagtgcggt ggtcaacatt ttaaccagat cgtagcacag ccaacctggc tgtggggcaa
     2821 ccaagcgtct cgcttggtca tctgacaggc tggaagcccg tcggcgccac ggcattgaat
     2881 tcgcttttca gcggagaaaa gttgtgcagc gaactggtat gggtaatttt tggctttcac
     2941 tgcgatccca acagaacgat gccctcatcc tgccgaatct gctgccttcg taccgtgttc
     3001 catttagcac cgttagctca attggcagag caagtgactc ttaatcactg ggttgcaggt
     3061 tcgattcctg cacggtgcag tgtttcgtcg gcgtcgcgga gcacggccct atgatttatt
     3121 ggctcgtcgt ggctgccata tgcgcgttaa ttgcctacgg caggtttatg ctaagacctt
     3181 ttcgacccct ctccaacccc gcggcccgtc gccggatcga cgccgggcct gaaaaagcaa
     3241 attcctaatc acttcgaaat gtcccatggc tcagaagatc gagttgcacg tcaatggccg
     3301 tgcccacaac gtggaggtgg agccggagac gccgttgctt tacgtcctta ggaactatct
     3361 ggagctgaac tggccgagat acggctgcgg ctacgagcag tgtggcgcgt gcatggcgct
     3421 cgtcaatggc gtgccgcttc ccacctgcaa gatgccggtg gccagcgccg tcggcaaaac
     3481 gatccgaacg ctcgaggatc tcgggacgga agagaagccc catcccctgc agcgcgcctt
     3541 cattcaggag caggcggcac agtgcggata ctgcatcagt gga
//
LOCUS       DASQUQ010000156         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_680382, whole genome shotgun sequence.
ACCESSION   DASQUQ010000156 DASQUQ010000000
VERSION     DASQUQ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680382"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="dapA"
                     /locus_tag="VE486_04460"
     CDS             <1..786
                     /gene="dapA"
                     /locus_tag="VE486_04460"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZA38372.1"
                     /translation="AGITGIVAVGTTGESPTLSHEEREQVIRLAVTTANKRCQVLAGT
                     GSNATQHAVADTKMAEKLGADGALLVAPYYNKPSQEGLFRHFKTIADATSLPIVLYNI
                     PGRCGVDIAPDTVARLAKESRNIVSIKEASGSVERISELRMRLPDSFTILSGDDSLTL
                     PFLSVGAVGVVSVASNLFPVEVCALVRAFESGDVKSATSLHRKMFGLFKDLFIEPNPV
                     PVKTALGWRGVMSGEVRLPLCEMSEGNQARLRRTLEVFERGSP"
     gene            783..1472
                     /gene="dapB"
                     /locus_tag="VE486_04465"
     CDS             783..1472
                     /gene="dapB"
                     /locus_tag="VE486_04465"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZA38373.1"
                     /translation="MTNPPVRVLLVGAAGRMGKTIVDVAKGDPNIEIVAQCDIGDALG
                     PAMRKSDVVIDFSHPDAIDGICDAASQDQRPLVIGTTGHSVERRSAIERAAKSVPVVF
                     ASNFSAGVNALFALARNTAETLGPEFSVEIIETHHQMKKDAPSGTAKTLAAILRKARN
                     ASDEIPIQSIREGDVVGEHTVIFSGPDERLELCHKASSRAAFARGALHAAQWVIGKSP
                     RLYSMQDVLGL"
     gene            1540..2049
                     /gene="folK"
                     /locus_tag="VE486_04470"
     CDS             1540..2049
                     /gene="folK"
                     /locus_tag="VE486_04470"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZA38374.1"
                     /translation="MRTAVALGSNIGDRLENLRAARRQILDLTDIQPPVLSSPIYETD
                     PVDCEPEAPKFLNAVIEFGYNGDPRQILKKLKDIEEVLGRPREHARNVSRKIDIDLLY
                     IGDTKINERELELPHPRMHLRKFVLQPLADIQPGLILPGRTKTVWELLAQLNDTCNVV
                     CLRDDWESQ"
     gene            2046..2807
                     /gene="panB"
                     /locus_tag="VE486_04475"
     CDS             2046..2807
                     /gene="panB"
                     /locus_tag="VE486_04475"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZA38375.1"
                     /translation="MKDFREMKRRGERITALTAYDYPTARLLDESGIDIILVGDSLGM
                     VVLGYQDTTNVTLEEMLHHTRAVARGVKQALLVADMPIDTYDTAEQAVDTARQFITAG
                     AQAVKLEGGASHVVQIEAITRAGIPFMAHIGMLPQSVREEGGYKIKGRTQSEAEALIA
                     DAHAVEKAGAFSVVLEIVVADITKQITNEIAIPTIGIGSGDHCDGQILVTHDLIGLFP
                     WFTPKFVSPQARVAEEIRRAVRVFIERTRGSSIGG"
     gene            2963..>3174
                     /locus_tag="VE486_04480"
     CDS             2963..>3174
                     /locus_tag="VE486_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38376.1"
                     /translation="MRVSDFDDFDNLETQGLLAGSKERSWLWFGLIVSLGLHAALCTY
                     FYRTHFQTVAAVIVPSEQTPTFKVRN"
BASE COUNT          738 a          837 c          912 g          687 t
ORIGIN      
        1 gccgggatta ccggaatcgt cgcagtcggg acgaccggcg aatcgccgac gctttcgcac
       61 gaggaacgcg aacaagtgat ccggctggcg gtcacgaccg caaacaaacg ctgtcaggtt
      121 cttgccggca caggttccaa cgccactcaa catgcggtgg ccgataccaa gatggccgaa
      181 aaactcggcg cagatggcgc gctactcgtc gctccatatt acaacaagcc gagtcaggaa
      241 ggtttattcc ggcatttcaa aacaatcgct gacgcaacct cattacccat cgtgctctac
      301 aatattcctg gccgctgcgg cgtggacatc gcgccggata cagtggcgcg tctcgcgaag
      361 gaatcccgga atattgtttc gatcaaggaa gccagcggga gtgtcgagcg catcagcgag
      421 ctgcggatgc gtctgcccga ctcatttacc attttgagcg gtgacgattc cctgactctt
      481 ccgtttctgt cggttggagc agtcggggtg gtcagtgtag cctcgaattt attcccggtg
      541 gaagtttgtg cactcgtccg tgctttcgaa tcaggcgatg taaaatcagc gacgagtttg
      601 caccgtaaaa tgtttgggct ctttaaagat ctattcatcg agccgaatcc ggtgccggtg
      661 aaaacagcgc tcggctggcg cggcgtaatg tctggtgaag ttcgcctgcc cctgtgcgaa
      721 atgagcgaag gcaatcaggc gcgtttacgc agaacgctcg aggtcttcga gcggggatcg
      781 ccatgacgaa ccctcccgtc cgggtgttgc tcgttggcgc ggccgggcgc atgggaaaga
      841 cgattgtcga tgttgcgaag ggcgatccga acatcgagat cgtggcacaa tgcgatatcg
      901 gtgatgctct tggaccggcg atgagaaaat cagatgtcgt gatcgatttt agtcatccgg
      961 acgcaatcga tggaatttgc gacgctgcat cacaagatca acgtcctctc gttatcggaa
     1021 caaccggaca ttccgtagaa cggcgatccg ctattgaacg agccgccaaa tccgttccgg
     1081 ttgttttcgc gtcgaacttc agcgcaggcg tgaacgcgct tttcgctttg gcccgcaata
     1141 ctgcggaaac gcttggccct gaattcagtg ttgaaattat tgaaacgcat catcaaatga
     1201 agaaagacgc gcccagcgga acagcgaaga ccctcgccgc cattttgcgc aaggcgcgga
     1261 acgccagtga tgagatcccg attcagtcga tccgcgaagg cgatgtggtg ggcgaacata
     1321 cggtgatttt ttcgggaccg gacgagcgac tcgagctttg tcataaagcc agcagccgcg
     1381 ctgcttttgc gcggggcgcc ctacacgcag cgcagtgggt gattggcaaa tcaccgaggc
     1441 tttactcgat gcaagatgtg ctgggattat aaagctggag cggcgctctg tgagcgtccg
     1501 acgaattagt gcggcgatca cagaccgccg ctacaaaaaa tgagaaccgc ggttgcgctt
     1561 ggatcaaaca ttggggatcg actcgagaat ttgcgcgccg cgcgacgaca aattcttgat
     1621 ctcacggata tccagccacc cgttttgtct tcgccaattt acgaaaccga tccggtagat
     1681 tgcgaaccag aggcgcccaa attcctaaat gcggttattg agttcggtta caatggcgat
     1741 ccgcgacaaa ttttaaaaaa attgaaagac atcgaggagg tcctcggccg gccgcgcgaa
     1801 catgcgcgga acgtttctcg caagatcgac atcgaccttc tctacatcgg cgacacgaaa
     1861 atcaacgaga gagaattgga attaccgcac ccgcggatgc acttgaggaa attcgttctt
     1921 cagcctctcg ccgacatcca gccgggattg attttgcccg gccggaccaa aaccgtttgg
     1981 gagttgctcg cgcagcttaa tgacacatgt aacgtagttt gcctcaggga cgactgggaa
     2041 tcgcaatgaa agattttcgt gaaatgaagc gtcgcggtga acgcatcact gccctgacag
     2101 cttacgatta cccaaccgcg cgcctgctcg acgaaagcgg gatcgacatc attctggtgg
     2161 gcgactcgct gggcatggtt gtgcttggct accaggatac cacgaatgta acgctcgaag
     2221 agatgctgca tcacacgcgc gcggtggctc gtggtgtgaa acaggccctg ctcgtcgccg
     2281 atatgccgat tgatacttac gacacggccg agcaggccgt agacacggcc agacaattta
     2341 tcactgccgg cgcgcaagcg gtgaagctgg aaggcggcgc gagccatgtc gtgcaaatcg
     2401 aagcgatcac gcgggcggga attccgttta tggcacacat tggaatgttg cctcaaagtg
     2461 ttcgggagga aggcggatat aagatcaaag gccgcaccca atctgaagcg gaggccctga
     2521 ttgcggatgc gcatgcggtg gaaaaagccg gcgcgttttc ggtcgtattg gaaattgtcg
     2581 tcgcagacat tacaaagcaa atcacaaatg aaattgccat cccgaccatc ggcatcggat
     2641 caggcgacca ttgcgacgga cagatcttag tcacgcacga tttaatcggt ttgtttccct
     2701 ggttcacacc gaagtttgtc tcgcctcagg cgcgtgtggc ggaggaaatt cgccgcgctg
     2761 tcagagtttt tattgagcga acgcgaggca gcagcattgg aggataggct tccagcctgt
     2821 ctcggcagac gggcatcttg cctgtcggcg aatctaagaa gcaacaggct gggaagcctg
     2881 tctgccaaga cagccaagat ggctattttc cgtccgtacc gtggctcgcg cgttcaaatg
     2941 tgttatttta caaccgaggg aaatgagagt ttctgacttc gacgatttcg acaatcttga
     3001 gacccagggt ctgctcgcgg gcagcaaaga gcgcagttgg ctctggttcg gattgattgt
     3061 ttctctggga ctgcacgccg cgctctgcac ctatttttac cggacacatt ttcaaactgt
     3121 ggccgccgtg atcgtcccga gtgagcaaac gcccaccttc aaggtcagga atat
//
LOCUS       DASQUQ010000157         8046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_501544, whole genome shotgun sequence.
ACCESSION   DASQUQ010000157 DASQUQ010000000
VERSION     DASQUQ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501544"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VE486_04485"
     CDS             <1..483
                     /locus_tag="VE486_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZA38377.1"
                     /translation="RILRCARRSAVHGNNLRHRAVHILIFDQAGEVYLQQRSRWKDRH
                     PLRWDSSAAGHVATGESYEETARRELEEELGINVPLEKILELPASAHTEQEFVWLYRG
                     KIRGNPIINPREIETGTFFSPAIIDGWVTARPENFAPGFVECWKAYRGKKGANSDTFT
                     "
     gene            complement(768..1508)
                     /locus_tag="VE486_04490"
     CDS             complement(768..1508)
                     /locus_tag="VE486_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZA38378.1"
                     /translation="MCLLALLASSAYAAEGVPDDCSQLIVAVAPTWDSARGELRLFER
                     SPGGNWEAVSGPLPVLFGKKGIAWGTGLAGQNEPGLRKKERDGRAPAGVFEIGKVFGY
                     DANLPAGADYPYHQVTEADVWSDDPRSPNYNRHIVIDPKNPPNNYRHEKMRSGDFAYH
                     WLIEIRHNSDPPIPGAGSAIFFHIRRGVNRPTTGCTTMAKTDLVKLIVWLRANRHPCY
                     ALLPAMEYDKKWRSWNLPSQETFRRSRP"
     gene            1694..2626
                     /locus_tag="VE486_04495"
     CDS             1694..2626
                     /locus_tag="VE486_04495"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZA38379.1"
                     /translation="MKTLFSTGAAIAILGLAAAPISGQTQSTTTTSTSYVQTSKLVGT
                     NVKSSQGEEIGVIKDVVLDRNTGCMAYTVLSTGGTGTRVTSGSKTVAVPWAVYSPTSD
                     LSVLTVTVDRERIYNAPVFDYARINEYTTTNYINNVYSYYGVSAGVGVSGATTTTTGV
                     AGAAGAPASPGASPTAALSPTAAGSPAATGSPATTASPAGRGSPTPHGTAAASPRATA
                     PTRPHATASPRANGAATASPSERGTRGARSRPGASPSPGDRSETTPPSEEERAPSEAT
                     ESPSEGKKSTRQRKTERSPEGEPSATPPSPEEQE"
     gene            complement(2738..4036)
                     /locus_tag="VE486_04500"
     CDS             complement(2738..4036)
                     /locus_tag="VE486_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZA38380.1"
                     /translation="MIYNRHGANCNRQLSMETNARPAPGGRSALILLLAINLFNYIDR
                     YVLVGVEPEIRTHFFRPDDPNAHALVGLLGTAFLVSYMVSAPIFGWLADRMSRWVIIG
                     VSVILWSAATAMSGLAGTFVLLLITRLFVGVGEGGYGPAAPTIIADLFPLVSRGRMLA
                     YFYVAIPVGSAIGYAVGGFVAAHWTWQTAFFVVAPPGLLLGLVCFSRRDPRARVGSKK
                     EKQRVSWNDYLSLARTRSYVLNTLAMTALTFAIGGMSFWVPGYLAYRGLPPSSRIIFG
                     GLTVVAGLTATLAGGIAGDWLRKRFPSSYFLISGIGVIIAFPFSVVMLFTPFPAAWVM
                     MFVAVFFLFFNTGPSNTALANVTDPSVRATAFALNILIIHALGDAAAPPLIGTVADRT
                     NMNVAFLVVSAMMLVAGMLWLWGAKFLPSDTAAIEAKRTE"
     gene            4051..6240
                     /locus_tag="VE486_04505"
     CDS             4051..6240
                     /locus_tag="VE486_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZA38381.1"
                     /translation="MSTAERALYFLGRPLVRCVYRVTALGTDNLPKGGFLLLPNHITW
                     IDAIVLQLACPRPIRFIIDQEFYRKPILHPLLRTLRCIPIEARRSHSAIRAATEAIAE
                     GEIVCLFPEGALERAGTLLRLRRGYELIVRHANAPVVPVWLDQLWGSIFSFQGGKFFR
                     KLPKRIPYPVTIAFGKPLEADAADIAAVREELLKLGEFCFSRRPSLNRHLAEPCVRGL
                     KRSPFATAVIDGLDHSALSRSKLLGAATALSRYLRKEFPDERIAIVLPASKGAMVANL
                     AVALADKVPVDLNFTMGRAANESCCKRANLRVAISATPFMERVKDFPWPERVLKLDEL
                     MPRMKGQIIFWWMLSILVPAGLLLRLLKIPKEGGHKEAVLLFTSGSTGEPKGVVISHR
                     NVVGNVSQFRQLLDATKDDAILASLPFFHTFGSTVTLWYPLIEGVRIVTYPSPLEAVK
                     NAALIERYKLTLLLATPTFLRGYLRKVDPQQLRSLRLVITGAEKLPLDLAKSFEDRFK
                     QRVFEGYGLTETSPVVSVNLPEPQPTKPGEQVQPSSRLGSVGKMAPGIAAEIREPETD
                     RKLSLHETGMLWLRGVNIFEGYLHDPEQTAEVLRDGWLKTGDVGRFDEDGFLYIEGRL
                     SRFSKMGGEMVPHEAIEGRITDLLGLSGSDERPIAIMGVQDEAKGEALVLLSAVDVDL
                     AQLRDKLREAGIPNLWIPKQVQRVNAIPVLASGKLDLKRCKEVTKHQ"
     gene            6387..7040
                     /locus_tag="VE486_04510"
     CDS             6387..7040
                     /locus_tag="VE486_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZA38382.1"
                     /translation="MKLLFGYSSRLHVIGAVHLNRSGGFLLAANHISHFDPFIISSVV
                     RRKIDWMAMAEFFPYPVLGFLLRAVDAFPADRNRADRATIRSAIERLKKGRIVGLFPE
                     GGIRDGARSLLEGAPLRPGASTLAHIAGVPILPCVIVGSDRLYSKKRWLPLRRTPVWI
                     AFGNPIPHFPDHEKSAARACIESELAAAFKCLYAELHEKFSLAPDDLPHSPSERMTE"
     gene            7181..>8046
                     /locus_tag="VE486_04515"
     CDS             7181..>8046
                     /locus_tag="VE486_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38383.1"
                     /translation="MERYVAACEKLTAENFYAVPDDTETPRAFGVANRPGATITWRSP
                     INTEFPANNIARADFFASPRGWRAPTVLMLHALMSASHIGYRRCAARFNQLGWNACFV
                     HLPYHYSRVPRGYWNGELAITADLIRNAEGLRQGVIELRQLMAILRERGCQEFGVLGT
                     SYGGWIGALLAMVERDFRFVALMAPIVNVEHAIWESPAARFMRRELRRAKVDSSLVAR
                     HFHLSSPMHNQPLCDPTRVLFVAGEFDLIARPDHIEEIQQKWRGSELLRVRQGHFGYR
                     MIRETIAQLKERG"
BASE COUNT         1842 a         2249 c         2218 g         1737 t
ORIGIN      
        1 cgaattttac gatgtgcgcg gcgctcggcg gtgcatggga ataacttgcg ccatcgtgcc
       61 gtccacatcc tgattttcga ccaggcaggc gaagtctatt tgcagcaacg ctcgcgttgg
      121 aaagatcgac atcctctaag atgggattcg agcgctgcgg gacacgtagc gaccggcgaa
      181 agttatgaag aaaccgcccg acgcgagttg gaagaggaac tggggataaa tgttcctttg
      241 gagaagattt tggagcttcc ggcctcagca cacacggagc aagagtttgt ctggctttat
      301 cgaggaaaga ttcgcggtaa tcccataatc aacccacgcg aaatcgagac tggcactttt
      361 ttttctccag ccattatcga tggctgggtt acggcacgac cagagaactt tgcacccgga
      421 tttgtggaat gctggaaggc ctatcgggga aaaaagggcg ccaacagcga tacgtttact
      481 tagacccgat aaatttccgc ttaggcagac ggcgcagggt cgcgcgcttg aatttccgtg
      541 cggctcgcct cgctccttcc ttgccaccat agatgctgtc gctaaacatc cttgtaacta
      601 cctgatttcc gcgaaggaaa tggacctgaa aaccatgcgt ctgtctcttg gcgcgccgtt
      661 ttgtatcgat gcgccggatg ttggcttctg aatcagggtt ggggcgtctt cgcatgacag
      721 ttaacgagaa gctaacgaaa caatccgggg aatcaatgca tcagaaacta gggacggctg
      781 cggcgaaacg tttcttgaga aggcaggttc caggaacgcc attttttatc gtactccatg
      841 gcaggtaaaa gcgcataaca ggggtgccgg ttcgcacgta accacacaat taactttaca
      901 aggtcggtct tggccatcgt ggtacagcca gtggtgggcc ggttaactcc gcgccggatg
      961 tgaaagaaaa tggcgctgcc ggcgcccggg atcggcggat cggaattgtg ccggatctcg
     1021 atgagccagt gataggcgaa atcgcccgag cgcatttttt cgtgcctgta attattgggc
     1081 ggattcttgg gatcaatcac gatatggcga ttataattgg gcgatcgcgg gtcatcgctc
     1141 caaacgtccg cttctgtcac ttggtggtaa ggataatcgg cgcctgcagg caaattcgcg
     1201 tcatatccga acaccttccc gatctcgaat actccggcag gcgctcgacc atctcgttcc
     1261 ttttttcgaa ggccaggctc gttttgtccg gcaaggcccg ttccccaggc aattcccttt
     1321 tttccaaaca acaccggtaa gggtccgctc acggcttccc aatttccacc gggcgatcgc
     1381 tcaaataatc gtaattctcc ccgggctgag tcccacgtcg gagcgacggc aacgataagt
     1441 tggctacagt cgtccggaac tccttcggca gcgtaagcac tactcgccag cagggccaac
     1501 aagcacatta gcaattgcct taagactttt cctgaaggga gcctaaggat cgacagaaat
     1561 gctctatcag aatgagaatg cattctacca attctacctt ttatttttga acaactctac
     1621 atccgcagcg aagagatgta gcgattcgtt cttaccggct ctcatcagag cgagccgaaa
     1681 tttcctaaca cttatgaaaa cattattctc aactggagca gcgattgcca ttctgggttt
     1741 ggccgctgct cccatatcgg gtcagaccca gagcactacg actaccagta ccagttacgt
     1801 tcaaaccagc aagcttgtcg gcaccaacgt gaaatcatca caaggtgaag aaattggcgt
     1861 aattaaagat gtcgtgcttg accgcaatac cggttgcatg gcttacaccg ttctctccac
     1921 aggagggacg ggaacacgtg ttaccagcgg tagcaaaact gttgcagttc catgggcggt
     1981 ctattctccc acctcagatc taagcgttct taccgtgacg gtggatcggg aacggatcta
     2041 taacgcgcca gtgttcgact acgcgcgcat caatgaatat acgaccacga attatatcaa
     2101 taatgtttac tcctactacg gcgtgagcgc cggtgtgggg gtttccggcg caacaactac
     2161 cacgacgggt gtggctggag cagccggcgc gccagccagt ccgggcgctt cgccaactgc
     2221 ggccctttca cccacggctg ccggatcgcc tgctgctacg ggttcacctg cgacgacggc
     2281 ttcacctgct ggcagaggat cgccaactcc acacgggacc gcagcggcaa gcccacgcgc
     2341 aacggcaccg acacggccac acgctacggc gtctccaagg gcaaatggcg ctgcgaccgc
     2401 gagtccgtcg gagcgaggaa cccggggcgc caggagtagg ccaggtgcat caccgtcgcc
     2461 gggtgaccgc agcgagacga caccgccaag cgaggaagag cgcgctcctt cggaagccac
     2521 agaaagtccg agcgaaggaa agaaatcgac ccgacagcgc aagacggagc gaagccccga
     2581 aggcgagcct agcgcgacac caccaagccc tgaagagcaa gaatagcgaa ccttctccgt
     2641 cgttcgtagg ttccaaaaca tcacaatcgg gtggggacag cgcgaagtct cctcgcctgt
     2701 tgcccgacga acggttgtgc ccgccctgac cgatgaatta ttcggttctc ttggcctcta
     2761 tcgccgcagt atcggatggc aaaaactttg cgccccagag ccaaagcata ccagctacca
     2821 gcatcatcgc cgacacgaca aggaaggcga cgttcatgtt tgtccgatcg gccacggtac
     2881 caatcaaagg cggagcagct gcgtctccca gcgcgtgaat gatcaaaata ttaagagcaa
     2941 aggcggtagc tctaaccgag ggatccgtaa cattggcgag tgcggtattt gaggggccag
     3001 tgttaaaaaa aaggaaaaaa accgcgacga acatcattac ccaggcagca ggaaacggag
     3061 tgaaaagcat tacgactgaa aatgggaagg caataatgac gccaatgccg gagatcagaa
     3121 agtaggagct gggaaaccgc tttcgcagcc aatcgcccgc aattccgcca gccagagtgg
     3181 ccgttaaccc ggcaacaaca gttaatccgc caaagatgat gcggctactc ggcggtaagc
     3241 cgcgatatgc aaggtatcct gggacccaaa aggacattcc cccgatcgcg aacgtgagtg
     3301 cagtcattgc cagcgtgttc aatacgtagg agcgcgtgcg ggcaagactg aggtaatcat
     3361 tccagctcac tcgctgcttc tcttttttgc ttcccactcg cgcgcgcgga tcgcgtcgcg
     3421 aaaaacatac cagtccaagg agcaatcccg gtggcgcaac aacaaaaaac gctgtttgcc
     3481 aggtccaatg tgccgcgaca aagccaccaa ccgcatatcc aatggcgctg cctaccggaa
     3541 tcgctacata aaaataggcc agcatgcgtc cgcgcgacac gagcgggaag agatcggcaa
     3601 tgattgtcgg cgccgccggg ccatatccac cctcgcccac tccgacaaaa agccgcgtga
     3661 tcaacaacaa gacaaatgtt cccgcgaggc cggacatcgc tgtcgccgcg ctccacaaaa
     3721 tcacgctcac gccgatgatc acccagcgcg acatccgatc ggcaagccag ccgaaaattg
     3781 gcgcgctcac catgtagctc actagaaaag ccgttccgag taaaccaacc agcgcatgcg
     3841 cgtttggatc gtcggggcga aaaaaatgcg tccggatttc cggttccact cccacaagca
     3901 catatctgtc gatgtaattg aaaagattga tcgcgagcag gagaatcagc gccgaacggc
     3961 cgccaggtgc aggacgtgca tttgtttcca tcgacaattg ccgattgcag tttgcgccgt
     4021 gccgattgta aatcataaac aatggcctcg atgtccactg ccgaacgcgc cctatatttt
     4081 ttaggccgcc cactggtgcg ctgtgtttat cgggtcacgg cgctcggaac tgacaacctt
     4141 ccgaaaggtg gatttttgct tctgccgaac catatcacat ggatcgatgc gattgtgctg
     4201 caactcgcct gcccacgccc gattcgcttc atcatcgatc aggaatttta tcgcaaacca
     4261 attctgcacc ccttgctgcg gaccctgcgc tgcattccaa tcgaggcgcg ccgttcgcac
     4321 tcggccattc gcgccgcgac ggaagcaatt gccgaaggcg agatcgtctg tctttttccc
     4381 gagggcgcgc tggaacgcgc cggaacatta ttgcggctgc ggcgcggtta cgaattaatc
     4441 gtgcgccacg ccaatgcccc agtcgtcccg gtctggctcg accaactctg gggctcgatc
     4501 ttttcgttcc agggtggaaa atttttcagg aaattgccga agcgaatccc ataccctgtc
     4561 acgatcgcat tcggtaagcc gctcgaagcc gatgccgccg atatcgcggc cgtgagggag
     4621 gagttgctca agctcggcga attttgtttc agccggcggc catcgctaaa tcgacatctc
     4681 gccgaaccgt gcgttcgcgg attgaagaga agccctttcg cgacggccgt tattgatgga
     4741 ctcgatcaca gcgctttgag tcggtcgaaa ttgctcgggg cggccacggc attgagccgt
     4801 tatcttcgaa aagaatttcc cgatgaacga atcgccattg tcctgcctgc aagcaaagga
     4861 gcgatggtag cgaacctggc ggtggcgctc gcggacaagg tgcctgtcga cctgaacttc
     4921 acaatgggac gggcagcgaa tgaatcctgt tgcaagcgcg ctaatctgcg ggtcgctatt
     4981 tcggccaccc cattcatgga acgcgtcaag gattttccgt ggccggaacg cgttctcaag
     5041 ctcgacgaat taatgccgcg aatgaaaggc cagatcattt tctggtggat gctgtcgatc
     5101 ctcgtcccgg ccggtttgct tttgcggctt ttgaaaattc caaaagaggg cggccacaag
     5161 gaagcggttc tgctttttac aagcggaagc accggtgagc caaaaggcgt cgtgatcagc
     5221 caccgcaacg ttgtcggaaa cgtctcgcag ttccggcaat tgcttgacgc gacgaaagac
     5281 gacgccattc tcgcgtcgct gccgtttttt cacaccttcg gatcgactgt cacgctttgg
     5341 tatccgctca tcgaaggggt gcgcattgtc acttatccaa gtccactgga agcggtaaag
     5401 aacgcggcgc tgatcgaacg ttacaagctg acgcttctgc tggcgacgcc tacttttctg
     5461 cgcggctatt tgcgcaaagt cgacccgcag caacttcgca gtttgcgtct cgtcatcact
     5521 ggcgcggaaa aacttccgct cgatctcgcg aaaagtttcg aggatcgctt caaacaaaga
     5581 gtttttgaag gttacgggct aaccgaaact tcgccggtcg tgagtgtaaa tctgcccgag
     5641 ccgcagccaa caaagccagg tgaacaagtc caaccgtcca gtcgccttgg ctcagttggc
     5701 aaaatggcgc cgggaattgc cgcggaaatt cgcgaaccgg aaacagaccg caaactttcg
     5761 ttgcacgaga ccggaatgct ctggttgcgg ggagtaaaca ttttcgaagg ttatctgcac
     5821 gatccggaac aaaccgcaga agtcttgcgc gatggctggt tgaaaactgg cgacgtcggc
     5881 cgattcgacg aggacggatt tctttatatc gaagggcggc tttcgcgttt ttcaaagatg
     5941 ggcggcgaaa tggtgccgca cgaagcgatt gaagggagaa ttaccgatct tcttggcttg
     6001 tccggaagcg atgaacgacc aatcgccatc atgggagtgc aggacgaagc caagggcgaa
     6061 gcgcttgtgc tgctcagcgc agtagatgtc gatctcgccc aattgcgcga caaacttcgc
     6121 gaagccggta tccctaattt gtggattcca aaacaggtgc agcgcgtgaa cgcgatcccc
     6181 gtcctcgctt ccggcaaact cgatttgaaa agatgcaagg aagtgacgaa gcaccaatga
     6241 cgaatgacca agcaagtccg aatgttcaga tgacgaaggc gcatccttgg tcatcaggct
     6301 ttcgtcattc attcgtcatt cggcattcgt cattcgtcat tcggtcggca tttcactccg
     6361 ctgcgaatcg tgctgctgct ctgttcatga agctgctgtt tggttactca tcgcgtcttc
     6421 acgtcatcgg cgccgtgcac ctgaatcgta gtggcggatt tttactggct gcaaatcaca
     6481 tcagccattt cgatccgttc atcatttcgt cagtcgtccg gcgcaagatc gattggatgg
     6541 caatggcgga attctttccc tatccagtgc tcggatttct cttacgagcg gtggatgcat
     6601 ttccggcgga tcgaaatcgc gccgatcgcg cgacaatccg cagcgcgatt gagcggttga
     6661 agaaaggccg aatcgtcggc ctttttcccg agggtggcat tcgggacggc gcgcgctcgc
     6721 tcctggaagg cgcgccgctt cggccgggtg catcaaccct tgcgcatatc gccggagtcc
     6781 caattctgcc gtgcgtgatc gttggcagcg atcgtctcta ctcaaagaag cgctggctgc
     6841 cgctgaggcg cacgcctgtc tggatcgcct tcggcaatcc gatccctcat tttcccgatc
     6901 acgaaaaatc tgccgcccgc gcgtgcatag agagcgaatt ggccgccgcg ttcaaatgtc
     6961 tgtatgctga gctgcatgaa aagttttcgc tcgcacctga cgatctgccg cattcaccta
     7021 gtgaacgaat gaccgaatga aatcacgggc gattgaaact ccacctgctc gagttcgcag
     7081 gttcgggcgt tttgcagcga gtagtgtcga cttgctcatg tgcgcgtcga tcaatctgct
     7141 tcagtcgcgc catcatttgc acgcgcgcag tcgtggagaa atggaacgct atgtggcggc
     7201 atgcgagaag ctcacggccg agaattttta cgctgtgcca gacgacactg aaaccccaag
     7261 agcttttggc gtcgctaacc ggcccggcgc cacaattaca tggcgcagcc cgatcaatac
     7321 cgaatttcca gcgaacaata tcgcgcgtgc tgattttttc gcatccccgc gcggctggcg
     7381 tgcgccgacg gttctcatgt tgcacgccct catgtcggcg agtcacatcg gttatcgccg
     7441 ctgcgctgcg cgtttcaatc aacttggatg gaatgcttgt ttcgtgcatc ttccatatca
     7501 ttattcgcgt gtcccgcgtg gttattggaa tggcgagctg gcgattacgg ccgatctcat
     7561 tcgcaacgcg gagggtttgc ggcagggcgt aatcgagttg cgccagttga tggcaatatt
     7621 acgcgagcgg ggctgtcaag agttcggtgt cctcggtacg agttatggcg gatggatcgg
     7681 cgcgcttctc gccatggttg agcgagattt tcgctttgtc gcactgatgg cgcctatcgt
     7741 gaatgttgag catgcgatct gggagagccc ggcagcacgc ttcatgcggc gcgaactccg
     7801 tcgggcaaag gtagactcct ctcttgtcgc gcggcatttt catttaagct caccgatgca
     7861 taatcagccg ctatgcgatc caactcgggt tctgttcgta gcaggcgaat tcgatttgat
     7921 cgcgcgaccc gaccatatcg aggaaattca gcagaagtgg cgcgggtcgg aattgctgcg
     7981 cgtccgccag ggccatttcg gttaccggat gatccgagag acaatcgcgc aactgaagga
     8041 gcgcgg
//
LOCUS       DASQUQ010000158         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1042080, whole genome shotgun sequence.
ACCESSION   DASQUQ010000158 DASQUQ010000000
VERSION     DASQUQ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042080"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1060)
                     /locus_tag="VE486_04520"
     CDS             complement(203..1060)
                     /locus_tag="VE486_04520"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZA38384.1"
                     /translation="MPVLTAPRFSIEAPFAREAESVRRVRTAWISDVHLGTRGANAGA
                     LLDFLREYEFETLYVVGDLIDIWQLRRGIYWPQEHNDVIQKILRKARKGTRTVYIPGN
                     HDELLSKFYGAYGRITVQKHAIHRLADGRRMLVIHGHEIDTVVQNVKWLAFAGDVGYQ
                     FLLSLNPLINFVRRRFGLGYWSLSAYAKKRVKDAVSFISRFEEEIVRYAKKFSVDAVL
                     CGHIHSAAIRRVGGVTYYNCGDWVESCSALVEHQDGVIEIIHFHPFLAARPAFNQPTA
                     LTPASISSR"
     gene            complement(1163..1978)
                     /locus_tag="VE486_04525"
     CDS             complement(1163..1978)
                     /locus_tag="VE486_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZA38385.1"
                     /translation="MKIKYLALSLAFVAAGLSSANADRLVIKGSDTLGAKLVPQLAEQ
                     FKAQHPGTTFDIAAEGSTTGIAAIIDGTAEIGMSSRRAKSSEVGAAAAKGKHMKPTVV
                     AYDGMAVIVNSGNPIKSLTKKQVEQIFTGDVTDWSAVGGSGGKISIYTRNTSSGTYSD
                     WKELAMKKRDYAGGSQKMAGNEQIAAEVGKNPNGVGYVGMAYTKASGVKVVPIDGTSP
                     SQQTVLAKTYPYARPTFYYTNGEPSGLAREFLDFTIGPAGQKIVARVGFVPIK"
     gene            complement(2024..3508)
                     /locus_tag="VE486_04530"
     CDS             complement(2024..3508)
                     /locus_tag="VE486_04530"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HZA38386.1"
                     /translation="MLVKKLTFAVVTFAFAFTGIAAHAQEAGALLDLLVRKRVITDQE
                     AEEVRSELSKEMAATSAGKWKLSAPINEIELYADTRVRYEYRGGRTASNDPENPNDWL
                     ERKRARYRVRVGLRGTLLDDWSFGLRLETSSSNRSTNVTFGGDSSNGPFSKNDDGIFV
                     GQAYIAYKGFPDITLTAGRMPQPLVTTSMLWDDDINPEGLAQQWKHTFNIDLGGGGGA
                     VESYSKEGYSKDGKGVVTTQAAAEPFKLKIDVFANFAQFVYDDTNPENPIGPARTTDS
                     NGFEQRVPTTDAYLLAWQVGARVTFPNNIYFQLAPTLYNYTGTGDTFNTFFQGDSPDD
                     NQTGINSLLVLDIPVELGWKIGELPMRMFGEFAVNLEGDDRAAAAGHPGSDGQRYAYQ
                     AGLAVGQLKAKSDWEIRSWWQHTDQYSVDPNLVDSDIFDSRVNMQGVAVKAGYALADA
                     IIFNLTWAYGWRSNGNFGTGGTGDIGINPLDQYQIFQADLNVKF"
     gene            complement(3821..4285)
                     /gene="secG"
                     /locus_tag="VE486_04535"
     CDS             complement(3821..4285)
                     /gene="secG"
                     /locus_tag="VE486_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZA38387.1"
                     /translation="MNLLINLCLAFFVLVALLMVLVILMQRPKSEGLGAAFGGAVTEN
                     IFGAQTTNVLVKFTSWLAGIFFALTFALSILYAHRSTADSAFRRELMKSQAAPQTSPA
                     PTAAPKPSPESSPATTPVQASPRSDASAAPDSGAGVLPAGSATPETSPSPGS"
     gene            complement(4402..>4975)
                     /gene="mazG"
                     /locus_tag="VE486_04540"
     CDS             complement(4402..>4975)
                     /gene="mazG"
                     /locus_tag="VE486_04540"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZA38388.1"
                     /translation="LIVMHAEIAREGGRFNIDNVLADVTEKLIRRHPHVFGKSDARDS
                     GAVLKQWESIKRTEKTNKHYLDGLPAALPALMRAQKAQSKVARVNFDWTDVRDVIAKV
                     GEELNEMKYAVASQDKQAIQDEIGDLLFAVVNLARKCKLDAESTLQAATDKFVVRFNR
                     LEDELHAQGKRLGDADLAELDEIWNEIKRK"
BASE COUNT         1011 a         1402 c         1362 g         1200 t
ORIGIN      
        1 aatgcttctg ctcttttcga ggaaacggcc gccacgcttt ccgccagggc gacttcgagc
       61 cgtgacgcgt acgctcgaat cgttttccgc aagtcacgcc gaagaacggc cagttctcct
      121 gcaagcattg tcacgggagt ggtttctcgc tgttttttcg gacgcttttt cggtcgcttc
      181 tttttcggtc gtcttttttt cttcatctgc tcgagatgga agccggcgtc agcgccgttg
      241 gctggttgaa agcaggacgc gcagctaaga atggatggaa gtgtattatc tctatgacgc
      301 catcctggtg ttcgaccagc gccgaacagc tttccaccca atcgccgcaa ttgtaataag
      361 taacgccgcc gactctacgg atggcggcgc tgtgaatgtg accgcacaga acagcgtcga
      421 ctgaaaattt cttcgcgtag cgaacgatct cttcctcgaa ccgactgatg aagcttaccg
      481 catccttgac tcgtttctta gcgtaagcgc taagcgacca gtaaccgagg ccgaatcggc
      541 ggcgcacgaa attgatcagg ggattcagcg acagtaaaaa ctggtaacca acatcgcctg
      601 cgaacgcgag ccacttcacg ttttgcacga ccgtgtcgat ttcgtgtcca tgaatcacga
      661 gcatgcgtcg cccatcggcc agacgatgaa tggcatgttt ctgcacggtg atgcgaccgt
      721 aggcgccgta aaatttggag agcagctcgt cgtggttacc cgggatgtat acggtccgag
      781 tccctttgcg ggctttcctc agaatctttt ggatgacgtc gttatgttct tgcggccagt
      841 aaattccgcg ccgtaactgc cagatgtcga ttaagtcgcc caccacatag agcgtttcaa
      901 attcatactc gcgcagaaaa tcaagtagcg cgcccgcgtt cgcgccacgc gtgcccagat
      961 gcacatccga gatccacgcc gtgcgcaccc gacgcacgga ttctgcttcc cgggcaaacg
     1021 gtgcctcgat ggaaaatcgg ggtgcggtca agaccggcat cgtgttttca aaaaaaatca
     1081 gcgcttaccg aagccatcat ttggcgaaaa gcccgccggg gcgctctccc ggcgggcgtt
     1141 cgcgaaccta acctgtcgtt ttttacttga ttggaacaaa gcctacgcgg gcgacgatct
     1201 tctggccggc gggaccgatc gtgaaatcga gaaattctct ggccaggcca ctcggttctc
     1261 cattcgtgta ataaaacgtc ggtcgtgcat agggataggt tttcgcgagc actgtttgtt
     1321 gggaaggtga cgttccgtcg atcggcacca ctttgacgcc ggacgccttg gtatatgcca
     1381 tgccgacgta gccgactcca ttgggattct tgcccacctc agccgcgatc tgctcgttgc
     1441 cagccatttt ttgcgaaccc ccagcgtagt cacgcttttt catcgccagc tctttccaat
     1501 cggaataggt gcccgatgat gtgttgcgtg tgtaaatcga gatttttcca cctgatccgc
     1561 caactgcgct ccaatcggtc acatcaccgg taaaaatttg ttccacctgc tttttcgtta
     1621 ggcttttgat tggattgccc gagttcacga ttacggccat gccatcgtaa gcgacgactg
     1681 tcggcttcat gtgtttgcct ttggcggcag cggcgcctac ttcggaactc ttggcgcgcc
     1741 tactcgacat tccgatttcg gcggtgccgt cgatgatcgc ggcgatgccg gtggtcgatc
     1801 cttccgcggc gatgtcgaag gtcgttccgg gatgttgcgc tttgaattgc tccgccagtt
     1861 gcgggacgag tttcgccccg agtgtgtcgg agcccttgat gacaagacgg tcggcatttg
     1921 cgctggacag gccagccgcc acaaatgcga gactaagtgc gaggtatttg attttcatag
     1981 gatttgggtg gtttgggttg ttcggtgata aaagttcgat cctttagaac ttcacgttca
     2041 aatcagcttg aaagatttga tattggtcga gtgggttgat accgatatcg ccagtgccgc
     2101 cggtgccgaa attaccatta ctgcgccagc cataggccca cgtcaggtta aagataatgg
     2161 catcggccag agcgtagccg gctttcacgg ctaccccttg catgttcacc cggctgtcga
     2221 agatatccga gtcgaccaga ttgggatcca ccgagtattg gtcggtgtgc tgccaccaac
     2281 tgcggatttc ccagtcgctc tttgccttta gctggcccac ggcgagacct gcttgatagg
     2341 cgtagcgctg accgtcgctc cccggatggc ctgccgctgc cgcgcggtcg tcaccctcga
     2401 gattgaccgc gaattcgccg aacatgcgca tcggcaactc cccgattttc cagccaagtt
     2461 ctaccggaat atccaacacc aggaggctgt tgatcccggt ttggttgtcg tcgggcgaat
     2521 cgccttggaa aaaagtgtta aacgtgtcgc cggtgccggt gtagttgtag agggtgggcg
     2581 cgagctggaa gtaaatgttg ttcggaaacg tgacccgcgc tccaacctgc cacgcgagca
     2641 ggtacgcgtc cgtggttgga actctttgtt caaaaccgtt gctgtccgtc gttcgggcag
     2701 gcccgattgg attttcagga ttcgtgtcgt cgtaaacgaa ctgtgcgaaa tttgcaaaga
     2761 catcgatctt caacttaaac ggttcagccg ccgcctgagt ggttacgacg cctttgccat
     2821 ccttgctgta gccctcctta ctgtaggatt cgaccgctcc gcccccaccg ccaagatcga
     2881 tattaaacgt atgcttccac tgctgggcca ggccctcggg attaatgtcg tcatcccaca
     2941 gcattgacgt ggtcacgagg ggttgcggca tcctgcctgc agtcaacgtg atgtcgggga
     3001 agcccttgta agcgatatag gcctggccta cgaagatgcc gtcgtcgttt ttcgaaaacg
     3061 ggccgttgct gctgtccccc ccaaacgtga cattcgtcga gcgattactg gagctggttt
     3121 ccaatcggag tccaaaagac caatcgtcaa gtagcgtgcc gcgcagacca acgcgcactc
     3181 ggtagcgcgc gcgcttgcgt tccagccaat catttgggtt ctccggatca ttcgaggccg
     3241 ttctgccgcc gcgatattcg taacgcacgc gcgtatcagc gtagagctcg atttcgttaa
     3301 tcggcgccga gagtttccat ttcccggcag aggtcgctgc catctctttt gacagctcag
     3361 accttacttc ttctgcttcc tggtcggtga tgaccctttt acggacgagc agatcgagca
     3421 gtgcgcctgc ttcttgcgca tgagccgcga ttccggtgaa cgcaaaagcg aaggtgacaa
     3481 cagcgaacgt taattttttt acaagcatta tttctcctta tttttatgcg gcactaggcc
     3541 gcgtttcttg tcgttgttcg ctgcgaaaat ggagggcaat tgttacagcc gtatggcggc
     3601 aaagtaacaa ttgcgtgaac tcgcaaatca ttgtagcgac gcgcatcgaa gctgtgcgtc
     3661 gtagcgcagc gcgacggggt gattggagat caactcgcga aagggctttc ggagttcgct
     3721 tgccgtccct gcccgaaagg gaggatctct accgttacgg ccttgtcgtg gcagagcgac
     3781 ttgcgacggg gtgcgacgga cgtccggcga accggtccgc ttacgaaccc ggcgatggcg
     3841 aggtctctgg cgtcgcgctg ccggcaggca aaacgcccgc gcctgaatcc ggcgcggcgg
     3901 aagcatccga cctcggtgaa gcctgaaccg gcgtagtcgc cggagaagac tccggcgaag
     3961 gtttaggagc agctgtcggt gcgggagaag tctgcggcgc cgcttgcgat ttcatcagct
     4021 cacgccggaa agcgctgtcg gccgtgctcc tatgcgcata cagaatcgac aacgcaaagg
     4081 tgagcgcgaa aaaaatgccg gccagccatg acgtaaactt caccagcacg ttggtggttt
     4141 gcgcgccgaa aatgttttcc gtaaccgctc cgccaaatgc cgcccccaaa ccctcactct
     4201 ttggacgttg catcaggata acaagcacca tcagcagcgc caccagcacg aagaacgcca
     4261 ggcataaatt gatcaggaga ttcatcggga cggagaatat gaatgacgaa tgtcgaatgt
     4321 cgaatgacga aacaggagcc cgcgcaaaaa cgacctcatc cgggcttcgc aattaattcg
     4381 tcattcgtca tttggttttc gtcatttcct ttttatctca ttccaaatct catccagctc
     4441 agccagatcg gcatccccaa gccgcttgcc ttgggcgtgc aactcgtcct cgagccgatt
     4501 gaatcgcacg acaaacttgt cggtagcggc ttgcagcgtg ctttcggcat cgagtttgca
     4561 tttgcgcgcg agattgacga ccgcgaacag cagatcgccg atctcgtctt gaattgcctg
     4621 cttatcttgc gaagcgactg cgtacttcat ttcattaagt tcttctccaa ccttcgcgat
     4681 cacgtcacgc acatcagtcc aatcgaaatt gacgcgcgca acttttgatt gcgccttttg
     4741 cgcgcgcatt aaggcgggca gcgccgccgg caggccatcg agataatgtt tgtttgtttt
     4801 ttctgtgcgc ttgattgatt cccattgttt cagcaccgcg ccgctatcgc gtgcatcgct
     4861 tttgccgaac acatgcgggt ggcgccggat gagtttctcg gtcacgtcag ccagcacatt
     4921 gtcgatattg aaccggccgc cttcccgcgc gatttcggcg tgcatcacaa tgagc
//
LOCUS       DASQUQ010000159         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_123741, whole genome shotgun sequence.
ACCESSION   DASQUQ010000159 DASQUQ010000000
VERSION     DASQUQ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123741"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VE486_04545"
     CDS             <1..214
                     /locus_tag="VE486_04545"
                     /inference="COORDINATES: protein motif:HMM:NF020717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase domain-containing protein"
                     /protein_id="HZA38389.1"
                     /translation="GAQSGLLFVLNNRSSWNGAWVQTRWNNTRFVPVAWRGRDDLGTP
                     QEKPTNDSGWVDLWAPPRGYVVYVPA"
     gene            254..487
                     /locus_tag="VE486_04550"
     CDS             254..487
                     /locus_tag="VE486_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38390.1"
                     /translation="MGPRDEHLVLHADGTAENWVVTASDRTEPTTGHWSVEGKTLKLS
                     FGDNENARPFTIYEGQLVFPNIPNRRQFWEKIE"
     gene            complement(506..1606)
                     /locus_tag="VE486_04555"
     CDS             complement(506..1606)
                     /locus_tag="VE486_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZA38391.1"
                     /translation="MKGNSLGQLILTGVPGTELDSQAERLFQRVQPGGFILFSRNIES
                     PVQLRKLIDDLRGLSEIEPIITIDQEGGRVSRLRLIGNEPPNANQLRDKNDIELIHRH
                     GDITGRLLRLFGFNLDLCPVLDISFDDDADNSLRGRCYGKTVDQVIRNAGAFNEAMRG
                     QGIASCGKHFPGYSAATVDAHHDLPKINRTREELDREELAVFRQFVGEIDSMMTCHGW
                     YPCFEPDRKPATLSHRVVRQLLREEMGFDGLIMTDDLDMGAILNEYGIDETIRLAIAA
                     GNDLAMICHRIPEIDNVHRILGTLPRVQIDGALASVGRFKEKLVPPDEFSETAFREID
                     SEIWDLRVAVLGEERARQRSPEDAKRSPVEMY"
     gene            1672..2235
                     /gene="nadD"
                     /locus_tag="VE486_04560"
     CDS             1672..2235
                     /gene="nadD"
                     /locus_tag="VE486_04560"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZA38392.1"
                     /translation="MKLGICGGTFDPIHHGHLILAREARELFGFEKVLFVPTAVSPFR
                     NPPVARAQTRLSMLRAAIEDEPGFEVDDCELRRPPPSYAIDTIEEIRTRERDAQIYYL
                     IGEDNVPTLPKWHRFDELRKAVNFIVLDRTGTPPDHPYPIVHRKIDISATEIRKRVAS
                     GQSIRYFVPQVVEEIIRREKIYQEQAK"
     gene            2232..2591
                     /gene="rsfS"
                     /locus_tag="VE486_04565"
     CDS             2232..2591
                     /gene="rsfS"
                     /locus_tag="VE486_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZA38393.1"
                     /translation="MTPENLAKACAELASNKKAEDIVVLDLRAISTFTDFFVICSATS
                     EPQLKAIANEIEMRLREDHSVRPVAIDGFPASQWIVLDYLQVVVHVFHRDKRAFYSLE
                     DLWGDAPSLQWEKAEAR"
     gene            complement(2654..>3102)
                     /locus_tag="VE486_04570"
     CDS             complement(2654..>3102)
                     /locus_tag="VE486_04570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZA38394.1"
                     /translation="TASGLQYKVIKEGTGAQPKATDTVSVNYRGTLIDGKEFDSSYKR
                     GQPATFPLNGVIKGWSEAVQLMKPGSKYQLFVPPDLAYGERAVGPDIPANSTLIFEVE
                     LLDVKPPATPAAGTSPAAPPPAQAAPKASLPSKPSPSPSPKASPKK"
BASE COUNT          689 a          866 c          846 g          701 t
ORIGIN      
        1 cggcgcgcaa agcggattac tctttgtctt aaacaatcgc agtagctgga acggcgcgtg
       61 ggtccagacg cggtggaaca acacgcgctt cgtaccagtc gcgtggcgcg ggcgcgacga
      121 tctcggaaca ccgcaggaaa aaccgactaa cgattccggc tgggttgatc tctgggcgcc
      181 gccacgaggc tatgtagttt acgttccggc ctgacccggg cggcggatca ggcttgctcg
      241 ccgacaggtc cctgtgggtc cgcgggacga acatctcgtc cttcatgctg acggcacggc
      301 ggagaactgg gtcgttaccg cttccgatcg cacagaacca acgaccggtc attggagcgt
      361 tgagggcaag acactgaagt tatcatttgg tgacaatgag aacgcccgtc cctttaccat
      421 ctatgagggg caactcgttt tccccaatat tccgaaccgc cgtcagttct gggagaagat
      481 cgaataggga gttgttctac ccttgttagt acatctcaac tggcgagcgt ttggcatctt
      541 ctgggctgcg ctgcctggcc cgttcttcgc caagaacggc tacgcgcagg tcccagattt
      601 cgctgtcgat ttcccgaaag gcggtttccg agaattcgtc cggtggcacc agtttttcct
      661 tgaagcgccc gacgctggcg agcgcgccat cgatttgaac acgcggcaat gtgccgagaa
      721 tgcgatgcac attgtcgatt tcgggaatgc gatggcagat catcgccaga tcatttcctg
      781 ccgcgatggc gagtcgaatt gtttcatcaa tgccgtactc attcaggatc gcgcccatgt
      841 cgagatcatc ggtcatgatc aaaccgtcaa agcccatttc ttcacgcagc agttgcctta
      901 cgacgcgatg tgacaaagtc gccggctttc tgtcgggttc aaaacacgga taccagccgt
      961 gacaagtcat catgctgtcg atttcgccca caaattgccg gaacacggcg agttcctcgc
     1021 gatccagttc ctcgcgagtg cgattaatct tcggcagatc gtgatgcgca tcgactgtcg
     1081 ccgctgagta accgggaaaa tgtttgccgc agctcgcgat cccctgccct ctcatcgctt
     1141 cgttaaacgc gccggcattt cgaataacct ggtccaccgt tttgccataa cagcggccac
     1201 gcagtgaatt gtcggcatca tcgtcaaatg aaatgtcgag taccgggcaa agatcgagat
     1261 tgaaaccgaa aagccgcaac aaacgcccgg tgatgtcgcc gtggcgatgg ataagttcga
     1321 tatcgttctt gtcgcgcaat tggttggcgt tcggtggctc attcccgatt aaacggagac
     1381 gcgaaacgcg gccgccttcc tggtcgatgg tgataatggg ctcgatctca ctgaggccgc
     1441 gcagatcatc gatcagcttg cgcagttgaa cagggctttc gatgttgcgc gaaaagagaa
     1501 taaaaccgcc gggttgcact cgctgaaata acctttcggc ttgagagtcg agttctgtgc
     1561 cgggaacgcc ggtcagaatt aattgaccaa gagagttacc cttcatactt tcggaactta
     1621 gatgtcgatg ttgcgcggag aaaatacaaa aacgcatctt ctcgcgccgt catgaaactc
     1681 ggcatttgcg gtggcacgtt cgatccgata catcatggac atttgattct tgcgcgcgaa
     1741 gcccgtgagc tattcggatt cgaaaaggta ctcttcgttc cgacagcggt ttcgccattc
     1801 agaaatcctc ctgtcgcgcg cgcacaaact cgcttgtcca tgctacgggc tgcaatcgaa
     1861 gatgaacccg gatttgaagt ggatgattgc gaactgcgtc gtccgcctcc ctcctacgcc
     1921 atcgatacaa tcgaagaaat ccggacccgg gaacgtgacg cgcaaatcta ttacctgatc
     1981 ggagaagaca atgtgccgac gctgccaaag tggcatcgct tcgatgagct gagaaaagca
     2041 gttaatttca ttgtcctcga ccgcaccggg acgccaccag atcatccgta tccgatcgta
     2101 catcgcaaaa tcgacatttc cgccacggaa atcagaaaga gagttgcctc cggccagtcg
     2161 attcgttatt tcgtaccgca ggtagtcgaa gaaatcattc ggcgcgagaa aatttaccag
     2221 gagcaagcga aataacgcca gaaaacctag caaaagcctg cgcggaactc gcctcgaaca
     2281 agaaggcgga agacatcgtc gtcctggatt tgcgcgcgat ctctacgttt accgatttct
     2341 ttgtgatttg ctcggccacg tctgagccgc agcttaaagc gatcgcaaat gaaattgaaa
     2401 tgcgattgcg tgaggatcat tcggttcgac ctgtggcaat cgacggtttc ccagccagcc
     2461 aatggatcgt gctggattac ctgcaggtag ttgtccacgt gtttcaccga gacaaacgcg
     2521 ccttttacag cctggaagat ctttggggcg acgccccatc acttcagtgg gaaaaggccg
     2581 aggcgcgctg aaatcgatgt agctccgggg agggcaggct ctgcgctccc cagaatttct
     2641 tgtgacgctt cgattatttt ttcggcgacg ctttcggcga tggcgatggc gaaggcttcg
     2701 acggaagcga tgcctttggc gctgcctgcg ccggcggtgg tgccgccgga gacgtgccgg
     2761 ccgccggagt tgccggaggc ttgacgtcca gtaactcaac ctcaaaaatc aacgtcgaat
     2821 tggcagggat atcgggaccc accgcgcgct cgccatacgc caggtccggg ggaacgaata
     2881 attgatattt cgaccccggt ttcatcagct ggaccgcttc gctccatccc ttgattaccc
     2941 cattaagcgg aaacgtcgca ggctgcccac gcttgtatga gctgtcgaac tctttgccgt
     3001 caatcagcgt gccgcgataa ttaacgctca cggtatcggt cgcttttggc tgcgcgcccg
     3061 ttccttcctt aatcaccttg tactgcaacc cgctggccgt cg
//
LOCUS       DASQUQ010000160         5109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_940978, whole genome shotgun sequence.
ACCESSION   DASQUQ010000160 DASQUQ010000000
VERSION     DASQUQ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940978"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..345)
                     /locus_tag="VE486_04575"
     tRNA            complement(255..345)
                     /locus_tag="VE486_04575"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Sec,seq:tca)
     gene            complement(425..610)
                     /locus_tag="VE486_04580"
                     /pseudo
     CDS             complement(425..610)
                     /locus_tag="VE486_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(905..2299)
                     /locus_tag="VE486_04585"
     CDS             complement(905..2299)
                     /locus_tag="VE486_04585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HZA38395.1"
                     /translation="MSSRRTEPLSIAAKLVLLFTLTATLLLSCGLGLFYWIVVRHAFA
                     EDNTFLADKISSLSADFKGGGPGTFGEEVRTRQAGEHVTYWIRLLDSGGRTATETPGM
                     GRLIPPDVFPPAQESTSSIRNPKDYRTGAKLFSLVAVNEKTGGQTYTIQVAQDQSSDE
                     QLERRFGILLGVMLAGGILASAIIAITVTKHGLRPLAEMTRSLERIGPTHLNERVAPA
                     GWPRELKPLAFAFDEMLSRLEDSFNRLSQFSADLAHELRTPVANIMGEGQVALTRERT
                     PDEYREAIESTIAECERLSGIVDNLLFVARVDAARESIERKLFDARAAVEKIATFYRT
                     LAEDRNVAIHCNGDGEIYADPVLFQRAVSNLVENALRFTPRGGTIQISIAVKPTHSEV
                     TVSDNGCGITPEHLPRVFDRFYRADSSRGSDGAGLGLALVKSIVELHGGSARIKSDVG
                     RGTTVTLSFPNKSV"
     gene            complement(2280..2951)
                     /locus_tag="VE486_04590"
     CDS             complement(2280..2951)
                     /locus_tag="VE486_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HZA38396.1"
                     /translation="MQILVIEDEKKTAAFLAKGLREAGFAIDLARDGESGLELARTTK
                     FDLLIVDIMLPNKDGWQVVEELRRGGMRTPILFLTARDSVRDRVKGLELGADDYLVKP
                     FAFSELLARVRSLLRRAPAEHEDSLRIDDLELDLKRHKAVRSGVPLNLTPKEFLLLAH
                     LVRSEGEVVSRAEIAEHVWDIGFRTDTNVVEVVVRRLRAKVDDPFETKLVHTIRGVGY
                     VLKAH"
     gene            complement(2976..3416)
                     /locus_tag="VE486_04595"
     CDS             complement(2976..3416)
                     /locus_tag="VE486_04595"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HZA38397.1"
                     /translation="MTVSRFRNILYIVIAIVSLAGLADAAYLTVQALTGETAACGGSP
                     DCFRVLGSSYAKVAGMPMAAFGSLAYFSVFSFATFAAFGYARARLFLILTVGAMFLVT
                     LWLLYVQAFLLHAYCRYCLFSAAITFLLAGLVIALPPSRDESDA"
     gene            complement(3642..4304)
                     /locus_tag="VE486_04600"
     CDS             complement(3642..4304)
                     /locus_tag="VE486_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZA38398.1"
                     /translation="MKRYLPFIIVAVAAIATLGSGAMLYRAKLPQPVTIPEDKSVSGK
                     SDAESMHIRGNADAPVTLEEFGDFQCPPCGSFAGFVDELVKEYDPHLRIVFRNLPLQM
                     HEHAREAALAAEAAGLQGRFWEMHDLLYREQATWSKADNPRELFDSYAGMIGLNLDQF
                     KKDMDGEKARARVDSDQERATSLGIKITPSVFINDRPISPTDKTPEGLRAAIDAALKE
                     KS"
     gene            4589..4936
                     /locus_tag="VE486_04605"
     CDS             4589..4936
                     /locus_tag="VE486_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZA38399.1"
                     /translation="MSACFSLGETQSGPGTGIEGVITFSPTQPGPIRADAPSSKPLAN
                     AAFVVENEKGAEVASITTDEQGQFRVSLLPGHYTVSKKGKKGGPGRYGPFDVDVVADK
                     MTRVTWECDTGIR"
BASE COUNT         1181 a         1432 c         1443 g         1053 t
ORIGIN      
        1 ccttgcgatc cagcgaaatg ggaagctgcg gcaaaacttg ccgcagggcc tgttggctca
       61 atttggaagc tcaccccgcg caggaagaga gcgaggtcaa tttgcgaatt tgctcacgcg
      121 acacccatct cacctccttt cgatccttgt cattccgagc gaagtcgagg aatcccgatg
      181 agttgcctgt tgatacggcc acgggctcct tcggctttgc ttcgctccgc tcaggatgac
      241 gagagaagat acgcggagag gataggaatc gaacctacct cgccacttgc gtggcgacga
      301 cggttttgaa gaccgcgagg gccaccaggc acccctcact ctccgagcac aaaaaggaaa
      361 cgtcgaacgc tcaacatcga acgcccaact ccgaagtaag gcaaaagata agcgattcga
      421 actgctactt gatctttttg atcgccttga cgatggcgtc gaaatccggc cagtcgccgg
      481 agtcgagttt ggaatggaga agtttgtcat tggcgcgaat ctcgaaggcg ccgccgctcg
      541 aggggataag cgtgagcgac ttgatgcgtt cgccgagctc aagaattttg gtcgccaaac
      601 tgacggcctt cggcgtgtag tttcaaacta cgcagtattc gatcgatacc tcggttttca
      661 ccaacgagct cctttccgaa atgaaactat cggtgcgatt aaatgaggtc aagaaatgtg
      721 cccgggataa catccgttcc gatgaacaga agtgtaaccg cagcccgttc atcccgggct
      781 gcggcttgct ccacttcaga gaccgcggaa acctgccgcg gtcttgcatc cctgaggcgt
      841 gaaccccaaa gaaaatgcga ctgtcatgtc aaaaggcgaa gttgatgccg cggcggacct
      901 accactacac ggacttgttt gggaaactaa gcgtgacggt tgtgccgcga cccacgtcgc
      961 tcttaatcct ggcggacccg ccatggagtt cgacgatcga ctttaccaaa gccagcccca
     1021 gacccgctcc atcggaaccg cgcgatgaat cggcacgata aaagcgatcg aatacgcgcg
     1081 gcaagtgctc cggcgttatt ccacatccgt tgtcgcttac tgttacttcg gaatgcgtgg
     1141 gctttactgc aatcgatatt tgaattgtgc cgcccctggg ggtgaagcgc agcgcgttct
     1201 ccaccagatt gctgaccgcg cgctggaaca acaccgggtc agcgtaaatt tcgccgtcgc
     1261 cgttgcaatg gatggcgaca ttccgatctt ccgcgagcgt tcgataaaac gtcgcgatct
     1321 tttccacggc ggcgcgagca tcaaacagct tccgctcgat ggattcgcga gccgcgtcca
     1381 ctcgcgccac gaagaggaga ttgtccacga tgcccgacag ccgttcgcat tcggcgatgg
     1441 tggattcgat ggcttcgcga tactcgtcgg gcgtgcgctc acgtgttaag gcgacctggc
     1501 cctcgcccat aatgttggcg accggcgtgc gcaattcgtg agcgagatcg gcggaaaatt
     1561 gcgagagccg attgaaggaa tcctcgagcc gggagagcat ttcgtcgaaa gcgaatgcga
     1621 ggggcttgag ctcgcgcggc cagccagcgg gcgcaacgcg ttcattgaga tgagttggcc
     1681 cgattcgttc gagtgaacgc gtcatttctg cgagtgggcg aagaccatgc ttagtgaccg
     1741 tgattgcaat aatggccgag gcaagaatgc cgcccgccag cataacgccg agcaaaatgc
     1801 cgaatcttct ttcaagttgt tcgtcgctcg actggtcttg cgcgacctgg attgtgtagg
     1861 tttggccgcc ggttttctcg ttcaccgcga ctaatgaaaa caacttggcg ccggtgcgat
     1921 aatcttttgg attgcgaatg gaggaagtcg attcttgcgc aggaggaaaa acgtctgggg
     1981 gaatcaaacg acccattccg ggcgtctcgg ttgcggtgcg gcctcccgaa tcgagcagcc
     2041 tgatccaata ggttacgtgt tcgccagcct ggcgggtccg cacttcctcg ccgaaagttc
     2101 ctggcccacc ccctttgaag tctgcgctta agctggaaat cttgtcggcc aggaaagtat
     2161 tgtcttcggc gaaggcatgt cgaacaacaa tccagtaaaa caagccgagc ccgcaggaaa
     2221 gcaatagcgt cgcggtcagc gtaaaaagca ggacgagctt cgccgcgatc gagagcggtt
     2281 cagtgcgcct tgaggacata acccacgccg cgaatcgtat ggactaattt agtctcgaat
     2341 ggatcgtcca ccttcgcccg caagcggcgc accacgactt ccacgacgtt cgtatcagtc
     2401 ctgaatccga tgtcccaaac gtgctcggcg atttctgcgc gcgatacgac ttcgccctcc
     2461 gagcgaacca gatgagcgag aagaagaaac tctttcggcg tcaggttaag cggcacgcca
     2521 gaacgaaccg ctttgtgccg cttcaagtcg agctccaagt cgtcgatgcg cagactatct
     2581 tcgtgttccg cgggagcgcg ccgcagcagc gagcgcaccc gggcgagcaa ttcggaaaac
     2641 gcgaacggct tcaccagata gtcgtccgcg cccaattcga gtcctttcac tcgatcacgc
     2701 acactgtcgc gcgcggtaag aaagaggatc ggcgtccgca ttccgccgcg gcgcagttcc
     2761 tcgacaacct gccagccatc cttgtttggc agcatgatat cgacgatgag aagatcgaac
     2821 ttcgtcgtgc gcgcaagctc caaaccgctt tccccatccc gcgcaagatc aatagcgaag
     2881 ccggcttcgc gcaggccttt ggccagaaaa gccgccgttt tcttttcgtc ctcgatcacc
     2941 agaatttgca tcgcgtagat gggacccctg tggtcctatg catccgattc gtcccgcgag
     3001 ggcggcagcg caatgacgag cccggcaagc aagaatgtga ttgccgccga gaaaaggcag
     3061 tagcggcagt aggcgtgcag caggaaggcc tgcacataca gcaaccagag agtgacgagg
     3121 aacatcgccc ctaccgtcaa aatgagaaat agtcgcgcgc gggcgtagcc gaaagccgcg
     3181 aacgtggcga agctgaatac gctgaagtaa gccagcgacc cgaatgcggc catgggcatt
     3241 cccgctacct tcgcgtagga gctgcccagc acacggaaac aatccggcga tcctccgcaa
     3301 gcagctgttt caccggttaa cgcctgcacc gtcagataag ctgcatccgc cagcccagct
     3361 agggaaacga tcgcgatgac gatgtaaaga atatttcgaa atcgcgatac ggtcatgatc
     3421 agaatgacga agcaccaatg acgaatgacg aaggaaggcc gaatgatcga atgacaaagt
     3481 ggtggctgac acgaatcaca cgaattgtca cgaattccat aaggcttaag tcaaccattg
     3541 agccaattga cgttcattcg tgagattcgt gtcttctgat ttcaatcatt ccttcgtcat
     3601 ttgtccgatt cgcgtaaatt cgcgtgattc gcgggcagcc tttacgactt ttctttcagg
     3661 gcggcatcga tggcggcgcg caggccttca ggcgttttat cagtcggact aatcggtcga
     3721 tcgttgataa aaacggaagg agtaattttg atccccagcg aagtagcgcg ctcctgatcg
     3781 gaatcgactc gggccctggc tttttctcca tccatatcct ttttgaattg atcgagattg
     3841 agcccgatca ttcccgcgta agaatcgaac aattcgcgcg ggttgtcggc tttgctccag
     3901 gtggcttgct cccgataaag gaggtcgtgc atttcccaaa aacgaccttg cagacccgcc
     3961 gcttcagcag ccagtgcggc ctcgcgtgcg tgctcgtgca tctggagcgg aaggttgcga
     4021 aagacaatgc gcaaatgtgg gtcatattct ttcaccaact catcaacgaa accggcgaag
     4081 ctgccacaag gcgggcattg aaagtcgccg aattcctcca gcgtgaccgg tgcgtcggca
     4141 ttgccgcgaa tgtgcatcga ttcggcatca ctctttccag agacgctttt gtcctcgggg
     4201 atcgtgacag gttgcggcag ctttgcgcga tagagcatcg ctccgctccc cagcgtggcg
     4261 atggcggcca cggcaacaat aataaaaggc agatagcgtt tcataaattg gcgcgagcgc
     4321 gagaagtttc agcgcaagag atgaatcgtc aagtgggggt ttggagccta accaccaatg
     4381 aacaccgaag cacacgaaca cggagaaaag atttgcccgc gaatcacacg aatttacgcg
     4441 aataagggag cgctgaaatg acgaaatctg aatgacccgc ctccgcccgg ctacggcgtg
     4501 gcattcgtca tattgacctg tgtttgcctt gagcgtaagt gaatacatga gatttgggaa
     4561 attctcgatc ctttcggggc tggtcattat gtcggcatgc ttttctttag gggaaacaca
     4621 atccggaccg ggaacgggga tcgaaggcgt gattacgttt agcccaactc agcctgggcc
     4681 gatcagggcg gacgctccca gttcgaaacc actcgcgaat gccgcgttcg tcgtagagaa
     4741 cgaaaaaggg gcggaggtcg cttcaatcac tacggatgag cagggacaat ttcgggtctc
     4801 cctcctgccg ggacattata cggtatccaa gaagggcaag aaaggcggcc ccgggcgcta
     4861 cggcccattc gatgtcgatg tcgtcgcgga caagatgacc agggtcacgt gggagtgcga
     4921 tacgggaata cgctgagccg ttgaaacttg cagggcgtct ttgtcagccc cgaatgcggt
     4981 tcggggtttc acaaaacgcc ctacaattct cagtcgctca gcggcgagtc caggcgctcc
     5041 acccccccgc gtagccgcag gcacccgcga aactcgcgag tgcctgcaaa tctgagcgtc
     5101 gcggctttt
//
LOCUS       DASQUQ010000161         8916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_682698, whole genome shotgun sequence.
ACCESSION   DASQUQ010000161 DASQUQ010000000
VERSION     DASQUQ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682698"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..873)
                     /locus_tag="VE486_04610"
     CDS             complement(205..873)
                     /locus_tag="VE486_04610"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZA38400.1"
                     /translation="MNTSGSYYLLLIAAVAVAFTCARLAGNEAAASSETMKVAFVVSD
                     NANVIDIAGAWEVFQDVMVGDRMPLQLFTVSETTAPIAMTGGLRIIPNYTFSSAPAPD
                     IVVVGAQKGTPGLAEWLRARALDSRVVMSVCTGAFKLGDAGLLDGKKATTHHDFWDAF
                     AKKFPHVQLKRGSRFVQSDDVVYTAGGLTSGIDLALHIVEKFYGRETADRTAAYMEYH
                     RSSR"
     gene            978..1955
                     /locus_tag="VE486_04615"
     CDS             978..1955
                     /locus_tag="VE486_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZA38401.1"
                     /translation="MKPNNVGILGFDGVAALDMIGPLEAFSVATVDDRRTRRQCYATS
                     IIGMSARSFVAESGVIFKPHRSFSDRYEIDTLIIPGGSGLRKVSINARVASWIASRAP
                     RIRRIASVCTGIYGLAPTHLLDGRHVTTHWRFTHDVARNFPKLKVDANALFRKDGKFY
                     TSAGVTAGIDLALALIEEDYGMRAALAVARELVVYVKRAGGQAQYSEPLQFQTESADS
                     FGDLIAWMRGHLQDDLSVETLAKRACLCARHFNRRFKSEFHQTPAAFVEDLRLSEARR
                     RLGTPGTTIDSVAASVGFRSADVFRRTFARRFDITPSDYRRRFKVPVGI"
     gene            1993..2562
                     /locus_tag="VE486_04620"
     CDS             1993..2562
                     /locus_tag="VE486_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38402.1"
                     /translation="MTQYLEGCMIFLVKRQFLSKALTPAIAASALILASCSTVETRIS
                     GNPEIYQSLSPSDQALVSQGQIRAGMPPNAVYLAWGSPDRKIIGNMRGRSTETWVYVN
                     YTTYPYSYPYPYYGPGYGLGYGFGLTRVGYRGRHHHHGGRSFVFFGSPFYDPFFYSYI
                     PPSIPYPYRTVTFANGRVVSFQYLVPPYR"
     gene            2620..4119
                     /locus_tag="VE486_04625"
     CDS             2620..4119
                     /locus_tag="VE486_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HZA38403.1"
                     /translation="MAYSSFRRFLDALEQAGELKRIPVPVDTDLLIAEWANREMKSPG
                     GGKALLFEHPIVDGKQSEFPIAINTMGSRRRMALALQVADIGDVAQEIQLILKAKPPT
                     DLREGWSLLKQGIHLLHARPKHVSEGACQEVVYEVGKRPTSNAQRPTHNDFTLHDLPI
                     LKCWPKDGGRFITLANVHTHDPETGARNVGIYRMQIYDERTTAMHWQIHKVGARHGKR
                     YYERGERMPVAVTLGGDPVYTFAATAPLPDGLDELLFAGFLRRKSIELVRCKTIDVDV
                     PADADFVLEGYVQPGEMRPEGPFGDHTGFYTAVEDYPVFHLTAITHRRAAVYPTTIVG
                     IPPMEDYYIGDACVRIFLPVFKMNFPEIVDMTLPPEGVFHNLVFVSIRKQYPYQAFKV
                     MHGLWGMGQMMFSKYIVAVDEDCDVHNTSEVLFRLCANTDPARDTTVIKNPSDSLDHA
                     PSDQNIGSHMGFDATRKLPGENYRRPWPELLKMTEEAQALVDALQAQTR"
     gene            complement(4171..4560)
                     /locus_tag="VE486_04630"
     CDS             complement(4171..4560)
                     /locus_tag="VE486_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HZA38404.1"
                     /translation="MKKNFFTAVAVFAMSSFILSSCETPTGQGAGIGAATGAIIGGAA
                     TGHVRGAALGAAAGAAAGALTGRAIQEDQAASYGPPPPGGFPFARWTGTPGLYYSPYT
                     GRVYNLRGVPPGGLTRDVDTGRLFRKP"
     gene            complement(4597..5424)
                     /locus_tag="VE486_04635"
     CDS             complement(4597..5424)
                     /locus_tag="VE486_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HZA38405.1"
                     /translation="MRQSKSNLALAFISLGHERKRDITVFYAFCRVIDDIADSAELNL
                     AEKRSRLAAWREMLRAARTDEPSLAHDVRSLIDKYSLPVDMLEEIITGVETDLSISRY
                     ATFEELRVYCYRVASAVGLVSIEIFGYRNAHCKEYALELGLALQMTNIIRDVGKDLRQ
                     GRIYLPQEDLARFGYSESELQDRRHNDRFVRLMEFEAARAREFFSRAAAALPPEDRRA
                     MAPAEIMSSIYRGLLRRMEVDKFRVFEKEYRLGKLEKASRITAQLLRSFLNLSSNLV"
     gene            complement(5464..6642)
                     /locus_tag="VE486_04640"
     CDS             complement(5464..6642)
                     /locus_tag="VE486_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38406.1"
                     /translation="MSPNISRIYPLLGLCAGYALVMFLNPLRPALGDGFRCIGRYKRI
                     WLTFAFLGFGYFVFQFATFTPIRNWADLDPNQVVSLPHWYWPRFVEIWKETPLPVLEG
                     VAGIFDNATTTYPLSIVAAVFMIVNWRGLHGALLRALRKRYRLWGYLIYLILLLSALA
                     SLFKPIVFWRLSEWSGLVSAAGLLRISATVDTVAFIFEYLLGVYIQVYLITVCLAWIK
                     GVSFEEGELFRFAMRRFSYVLEWAGIVVVVSTLVVRLPLLLAYFTNIPGVLDYLPIQR
                     MFMSGLIIAFCSVQISLALHNETLTEAIRAHRRFIRENAGRFAWFLVICGIHFFGIML
                     CDAIVRSAIADRLGALFIWKFIFACLRGIVTGWLLASWVCLFRQYESSRTKQEKWIQY
                     "
     gene            complement(6800..7891)
                     /gene="lepB"
                     /locus_tag="VE486_04645"
     CDS             complement(6800..7891)
                     /gene="lepB"
                     /locus_tag="VE486_04645"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZA38407.1"
                     /translation="MFTPRHVKHSRLLLRHARKYLRYKHDVLSGSDREEIVTEMENLR
                     SSLRQRDHERVHGLADILDKTLHRLTPVSWESHWRENCEVILVAIVVAVGIRSYFLQP
                     FKIPTGSMQPTLNGIIGHPSTEPPPNLLRQIGEFIALGRNYVNVVSREDDQVLQIEQK
                     KFLFFFTLSRLICEQQNFLVYAPPETLSHDFKVFPGRTYRRGEIIARGTVDTGDQVFV
                     DKFSYNFVKPHRGDVFVFRTNHIAGIREDPETGSPFYIKRLAGLPGDRLRINPPFLYI
                     NEKKAEGYGFARVMEAKPPYRGYGPGHDYLAEPNRSFTVPRNGYFALGDNSYNSYDSR
                     YWGAVPEENLVGRGLFVYWPFYPHWGLIR"
     gene            complement(8122..>8916)
                     /locus_tag="VE486_04650"
     CDS             complement(8122..>8916)
                     /locus_tag="VE486_04650"
                     /inference="COORDINATES: protein motif:HMM:NF018161.4"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HZA38408.1"
                     /translation="ALGFGFRCGFLGLLHMEIIQERLRREYNMDIIATSPSVVYEVVT
                     TRGERLLIDNPAHLPDPSTIQEIREPIVKAYVLCPNENIGDILQLILEKRGQMEHTES
                     LDTRRVMLHCELPLNEILVDFNDKIKSITRGYGSMDYEHHGYRPAKLVKLDLLVNGEP
                     VDAFSTIVHKDRAEARGRQLVAKLKEVIPRQLYQVAIQAAIGGKIIARESVSALRKNV
                     TAKCYGGDITRKRKLLEKQKEGKKRMKSIGKINIPQEAFIEVLKAQ"
BASE COUNT         2120 a         2474 c         2363 g         1959 t
ORIGIN      
        1 ccgcagcgtc tcttcgaaag tcaggatttc ctcgcgcggc aaccactcct gcaactcgtg
       61 cggcatgcaa taagtgcatc gctcattgca gcggtcggtg atcgagacgc gcagatagga
      121 aatccgatgg ccatgtcgat cctgcatagc agccaagatt aacgcgggct ggaggcgaat
      181 caaggtcgcc ggaccctcct cgaattaacg agatgaacga tggtattcca tgtaggccgc
      241 ggtgcgatca gccgtttcgc gtccgtaaaa cttttccacg atatgcaaag cgaggtcgat
      301 tcccgaagtg agtccgccag cagtataaac tacatcgtcg ctttgaacga atctgctgcc
      361 gcgcttcaat tgaacgtgcg gaaatttttt ggcaaaagca tcccaaaaat cgtgatgggt
      421 agtggccttc ttgccgtcga gcaatccagc atcgcccagt ttgaatgctc ctgtgcaaac
      481 cgacatcacg acgcggctgt ccaaagcgcg tgcacgtaac cactcggcca agccgggcgt
      541 tcctttttgc gctccgacaa ctacaatgtc gggagcgggc gcgcttgaaa atgtgtagtt
      601 tgggataatg cgaagtccgc cggtcatcgc tatgggcgcg gttgtctccg agacggtgaa
      661 taattgaagg ggcattcgat cacctaccat gacgtcctgg aacacttccc aggcgcctgc
      721 gatgtcgatt acgtttgcat tgtcggaaac gacaaacgcg actttcattg tttccgaaga
      781 agcggcggct tcgttacccg caagcctagc gcaggtgaac gccacggcca cagccgctat
      841 cagcagcaag taatacgatc cactagtgtt cattgaaatg ctccacaatt ttcgcaagga
      901 gtaggccgcc acgattaagt ccgcaatgac gttaatcaga tgatttcgga cttttgggga
      961 tatactctcc gacaatgatg aagccgaata acgttggtat ccttggattt gatggcgtag
     1021 cggcgctaga catgatcggt ccgctggaag ccttttccgt cgctactgtg gatgatcgaa
     1081 gaacgcgcag gcagtgctat gcaacgtcga tcatcggaat gagcgcacga tcatttgtcg
     1141 cggaatctgg ggtgatcttt aagccgcatc gttcattctc cgatcgatat gaaatcgata
     1201 cgctaattat cccaggcggc agcggccttc ggaaagtatc gatcaatgcg cgcgtggcgt
     1261 cctggatcgc gagccgagca ccccgcattc gccgaatcgc ctcggtgtgc accggaattt
     1321 atggattggc gccgacgcat ctgctcgacg gccgtcacgt caccacgcat tggcggttta
     1381 cgcacgacgt ggcccgcaat tttccaaagc ttaaagtcga tgccaatgcg ttgtttcgaa
     1441 aagacggcaa attttacaca tccgcaggcg tgaccgctgg aatcgatctc gctttggcgc
     1501 tcatcgaaga agattatgga atgcgcgccg ccctggccgt ggcgcgagag ctggtcgtct
     1561 atgtgaagcg agccggtggt caggcgcaat actccgagcc gctgcagttt caaacggaat
     1621 cggcggattc atttggcgat ctgatcgcat ggatgcgcgg ccatctgcaa gatgacctat
     1681 cggtggaaac cctcgcgaag cgcgcgtgcc tgtgcgcgcg gcattttaat cgacgcttca
     1741 aaagcgagtt tcaccaaaca ccggcggcat ttgtggaaga tttgcggctg agcgaagcgc
     1801 gacgacggct cggcacgcct gggacaacaa tcgacagcgt agctgcttcg gttggtttcc
     1861 gcagcgctga tgtatttcgt cgcaccttcg cacggcgatt tgatattacc ccgagcgatt
     1921 atcgtcgccg atttaaggtg ccggtgggca tctaacgccg ttgttgcatg ttcaatcagg
     1981 aaagcggggt gattgactca atatttggag ggctgtatga tttttctcgt gaagcgtcaa
     2041 ttcctttcca aggcgctgac gcctgccatc gcagccagtg cattaatcct tgcgagctgt
     2101 tccaccgtcg agacgcgaat ttctggaaat cctgaaattt accagagcct ttctcccagc
     2161 gaccaggcgc tggtaagcca ggggcaaatt cgcgcaggga tgccaccaaa cgcagtgtac
     2221 ctggcgtggg gttcccctga tcggaaaatt ataggtaata tgcgcggtcg ctcgaccgag
     2281 acctgggttt atgtaaatta tacgacttat ccttattcgt atccttatcc ctactacggt
     2341 cccggttatg ggttgggtta cggtttcgga ttgaccagag tcggttacag ggggaggcat
     2401 catcatcatg gtggccgttc cttcgtgttc tttggcagtc ccttctacga tccgttcttt
     2461 tattcgtaca tcccgccaag catcccctat ccctacagga cggtcacgtt cgccaatggc
     2521 agggtcgttt ccttccagta cctggtgcca ccgtatcgct aatcaaaaat tctgtcgcgt
     2581 tctggtaaag aacgcttcag tgtagcggga cgtcttgcca tggcttacag ctcattccga
     2641 aggttcctcg atgctcttga gcaggcaggc gagctgaagc gcatccctgt tccggtcgat
     2701 accgacctgc tcatcgccga atgggcgaat cgcgagatga aatcgcccgg cggcggaaaa
     2761 gcgcttcttt tcgagcatcc aattgtcgat gggaaacaat cggaattccc gattgcaatc
     2821 aataccatgg gatcgcggag acgaatggcg cttgccctgc aagtcgcaga catcggagac
     2881 gtcgcccagg aaattcaact catcctgaaa gcgaaaccgc ccaccgatct gcgcgaaggt
     2941 tggagcctgc tcaagcaagg aattcatctg ctgcatgcgc gcccgaaaca tgtaagcgaa
     3001 ggggcttgcc aggaagtggt gtatgaggtt gggaaacgcc caacatccaa cgcacaacgt
     3061 ccaacgcaca atgatttcac cctgcatgat cttccgatcc ttaaatgttg gccgaaggac
     3121 gggggacgtt tcatcacatt ggcaaacgtg cacacgcacg atccggaaac gggcgcacgg
     3181 aacgtcggca tttaccggat gcagatttac gacgagcgca cgaccgcgat gcactggcaa
     3241 attcacaagg tcggtgcgcg ccacggcaaa cgctattatg agcgaggcga gcggatgccg
     3301 gttgcggtga cactcggcgg tgatcccgtc tatacattcg cggcgactgc gccgctgccg
     3361 gatggtttgg atgaactcct ttttgctggt ttcctgcgaa gaaaatcaat cgaactcgtt
     3421 cgctgcaaaa caatcgatgt cgatgtgccg gcggacgccg attttgttct ggaaggttat
     3481 gttcagcccg gcgagatgcg tccggagggc ccatttggcg accacactgg cttttacact
     3541 gccgtcgaag attacccggt tttccatttg accgcgatca cccatcggcg tgccgcagtt
     3601 tatccgacaa ccatcgtcgg tatcccgccg atggaagatt actacatcgg tgacgcctgc
     3661 gtccggattt tcctgccggt attcaaaatg aatttcccgg aaatcgtgga tatgacgctg
     3721 ccgccggaag gcgtttttca taacctggtc ttcgtcagta ttcggaaaca gtatccctat
     3781 caggccttca aagtgatgca cggcctctgg ggcatggggc agatgatgtt cagcaaatac
     3841 atcgtggcgg tggatgaaga ttgcgatgtg cacaatacga gcgaagtgct cttccgtttg
     3901 tgcgcaaaca ccgatcccgc gcgagacacg acggtcatca agaatccaag cgactcgctg
     3961 gatcacgcgc cgagcgatca aaacatcggc tcccatatgg gatttgatgc cacccgcaaa
     4021 ttgccaggcg aaaactatcg ccgcccctgg cccgaactgc tcaaaatgac cgaggaagcg
     4081 caggcgttgg tggatgcgct ccaggcgcaa acacgctgac acatgtggcg ggggacgccg
     4141 cgctgcctct acccaagccg accgtagcaa ctacggtttg cggaaaagtc ggccggtatc
     4201 gacgtcgcga gtaagtccac caggcgggac tccacgaaga ttataaacgc gtcccgtgta
     4261 agggctgtaa taaagccccg gcgtgccggt ccagcgcgcg aacggaaaac ccccgggtgg
     4321 cggcggaccg taacttgcgg cttggtcctc ttggattgct ctgcccgtta acgccccggc
     4381 tgcagcgcca gcggctgcgc caagcgccgc gccgcgaaca tgaccggtag ccgcgccgcc
     4441 aatgattgcc ccggttgcag cgccaatgcc ggcgccttga ccagttggag tctcacacga
     4501 actgagaatg aaagaactca tcgcaaagac cgccacagct gtgaaaaaat tcttcttcat
     4561 aaatttaccc ttcattgacg aattaaaccg cgcaagtcag acaaggttgc tcgataggtt
     4621 caaaaaagac ctgagcaact gcgcggtgat ccgggacgct ttctccaatt tcccgaggcg
     4681 atattccttc tcgaacacgc gaaatttgtc gacttccatc cgccgaagta atccccgata
     4741 aatagagctc atgatctcgg cgggcgccat cgcccggcgg tcttccggcg gaagggctgc
     4801 cgccgcgcgc gagaaaaact cgcgcgcccg cgccgcttcg aactccatca gacgaacgaa
     4861 acgatcgtta tgtcgtcgat cctggagctc cgattcggaa taaccgaagc gagccagatc
     4921 ttcctgcggc agatagatcc ggccctgccg caaatctttg ccgacatcac ggataatatt
     4981 ggtcatttgc aaagccagac ccagctccag cgcatattct ttgcagtgcg cattgcgata
     5041 tccaaagatt tcaatgctaa ccaggccaac cgcgctggcg acccgataac agtaaacgcg
     5101 cagctcttcg aatgtagcat agcgcgagat gctcaggtcc gtttccaccc ccgtgataat
     5161 ttcctcgagc atgtcgaccg gcagcgagta cttatcgatc aaactccgta catcgtgtgc
     5221 aagagacggc tcatccgtgc gtgcggcgcg cagcatctcg cgccatgccg ccagccgcga
     5281 tcgcttctcc gcgaggttga gttccgccga atcggcgatg tcatcgatca cgcggcaaaa
     5341 tgcgtaaaag acggtgatgt ctcgtttgcg ctcgtgccct aacgaaataa atgccagggc
     5401 caggttcgat ttgctctggc gcataatttt cgccgcattc acgtgtctct taaaccggcg
     5461 gatttaatac tgaatccact tttcctgctt agtgcgactc gattcgtatt ggcgaaataa
     5521 acaaacccat gaagccagaa gccagcccgt aacaattcct cggagacacg caaagatgaa
     5581 tttccaaatg aaaagtgccc ccaaccggtc ggcgattgcg ctgcgcacga tggcatcgca
     5641 aagcatgatc ccaaaaaagt ggattccgca aataaccaag aaccacgcaa aacggccggc
     5701 attttcgcgg atgaaccggc gatgggcgcg tattgcctca gtcaacgtct cattatgcag
     5761 agcgagggag atctggactg agcagaaggc aatgatcaaa ccgctcatga acatacgctg
     5821 aatcggcagg taatcgagga caccgggaat gttcgtgaag taggcaagga gaagtggcaa
     5881 tcgaacgacc aatgtgctaa ccacgacaac tatgccggcc cactccaaga cgtaactgaa
     5941 gcggcgcatg gcgaagcgga agagttctcc ttcttcaaaa ctcacgccct ttatccaggc
     6001 cagacaaaca gtgatcaaat aaacctgaat gtagacgcca aggaggtatt cgaagatgaa
     6061 cgcgacggta tctaccgtgg ccgagattcg cagcagtccg gcggccgaaa caagaccgct
     6121 ccactccgat aatcgccaga atacaatcgg cttgaagagc gacgccaggg cgctcaaaag
     6181 cagaatcaag tagatgaggt aaccccacaa acgatagcgc tttcgcaagg cgcggagcag
     6241 cgcgccatgc aggccacgcc agttaacgat catgaagaca gcggccacga tcgaaagagg
     6301 ataagtggtg gtcgcgttat caaaaattcc agcgacgcct tccaacacag gcaacggcgt
     6361 ttctttccag atctccacaa atcgcggcca ataccagtgc ggtaacgaaa cgacctggtt
     6421 cggatctaga tcggcccagt ttcggatcgg cgtgaaagtg gcgaattgga aaacgaagta
     6481 gccgaatcca agaaaggcaa aggtgagcca gatgcgctta tagcggccga tgcagcgaaa
     6541 gccatctccg agggctggcc gcaagggatt aagaaacatc accagggcat atccggcgca
     6601 cagcccaagc agaggataaa tgcgtgaaat gtttggcgac atgcagcttc aatccaataa
     6661 gctgcgccag gaaaaatccg gaagtgatgt agtggcggag cagtggagta atggagaggc
     6721 cacaccgtcc gtcattgagg tccgcccatt actctcacac tccattactc cattactcca
     6781 tttctccact tccgctgccc tagcgaatca gcccccagtg cggatagaag ggccagtaaa
     6841 caaacaagcc gcgcccaacg agattttctt caggcaccgc tccccaataa cggctgtcgt
     6901 agctgttgta gctgttgtcg cctagggcga aataaccatt ccgtggcacg gtgaatgaac
     6961 gatttggttc cgccaggtaa tcatggcccg ggccatagcc gcgataaggc ggcttcgcct
     7021 ccatcacccg cgcgaaaccg tagccctccg cctttttttc attgatgtag agaaaaggcg
     7081 ggttgatgcg caaacgatca ccaggcaagc cggctagtcg cttaatgtaa aaaggcgacc
     7141 cagtctcggg atcctcccga atcccggcga tgtgatttgt ccggaaaaca aaaacgtcgc
     7201 cccggtgcgg tttaacgaag ttgtagctaa acttgtcgac aaacacctga tcgccggtgt
     7261 ccaccgtccc tctggcaata atctcgccgc gccgataagt ccgccccggg aaaactttga
     7321 aatcatgact gagcgtctca ggtggtgcat aaacaagaaa attctgctgc tcgcagatca
     7381 atcgcgagag ggtaaaaaag aagaggaatt ttttctgctc gatttgaagg acctgatcgt
     7441 cctcacgcga gacaacattc acatagttgc gacctaacgc gataaattca ccgatctgcc
     7501 gcaacaggtt cggcggcggt tctgtgctgg ggtgtccaat gattccgttt aatgtcggtt
     7561 gcatcgagcc ggttggaatc ttgaacggct gcaaaaaata tgagcggatg cccacagcca
     7621 caacaattgc caccaggatt acctcacaat tttctcgcca gtgcgattcc cagctgaccg
     7681 gcgtcagcct gtgcagcgtt ttatccagta tgtcggccag gccgtgtact ctctcgtggt
     7741 cgcgctgacg taaagaactg cggaggtttt ccatttcagt aacaatctct tcgcggtccg
     7801 agccactgag cacatcgtgc ttatagcgca gatacttgcg agcatgccgc agaagcagcc
     7861 tgctgtgctt aacgtgacgt ggcgtgaaca tggttctaaa tgacgaagcg cggatgacga
     7921 atgacgaaac aatgacgaag cgcgattgat cgaaaactac ggcgtgagac tattgttatt
     7981 ttcggtttac tcattcggat ttccttcgtc attcgtcatt cggatttcgt cattacgcgc
     8041 ctcattgcgc ctttaacact ccgataaaag cttcccgccc tacatcgtca ttccgagcga
     8101 cagcgaggaa tctcacaaac gtcattgtgc tttcaatacc tcgatgaacg cttcctgtgg
     8161 aatattgatt ttcccgatgc tcttcatccg cttctttcct tccttttgtt tctccagcaa
     8221 tttgcgcttc cgcgtgatat cgccgccgta gcatttcgcg gtcacatttt ttcgtaatgc
     8281 cgaaacgctc tctcgcgcaa taattttccc gccaatggct gcctggatcg ctacctgata
     8341 gagctgccgc ggaatcactt cctttaactt ggctaccagc tggcgccccc tcgcctccgc
     8401 gcggtctttg tgcacgatcg tcgaaaacgc atcgaccggt tcgccattca cgagcaggtc
     8461 gagctttacc aatttcgccg gacgatagcc gtgatgctca taatccatcg agccgtagcc
     8521 gcgcgtaata cttttaatct tgtcattaaa atcgacgaga atttcgttga gcggcagctc
     8581 gcaatggagc atcacacggc gcgtatcgag cgattcggtg tgttccatct ggccgcgctt
     8641 ctcgagaatt aattgcagga tgtcgccgat attttcattc gggcaaagca cgtaggcctt
     8701 tacaatcggc tctcgaattt cctggatggt gctcggatca ggcagatgcg cgggattatc
     8761 gatcaacagt ctttcaccac gcgtggtcac aacttcataa acgacgcttg ggctggtggc
     8821 gatgatatcc atgttgtact cgcgccggag ccgttcctgg atgatctcca tgtgaagcaa
     8881 accaagaaat ccgcagcgaa atccgaatcc gagcgc
//
LOCUS       DASQUQ010000162         8928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_763176, whole genome shotgun sequence.
ACCESSION   DASQUQ010000162 DASQUQ010000000
VERSION     DASQUQ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763176"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VE486_04655"
     CDS             complement(<1..1356)
                     /locus_tag="VE486_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38409.1"
                     /translation="MRRQPSRPHPAKNDLDLIPPEERRARRLRLLLFLAVPAAIALIA
                     ALYFAAPAVAGAIKGWQSRRLAQEAFALVNQKKWSEASAKARDAYLLRPSEPDSWRAI
                     ARVASRSGQWTAALEWWKKVDEAHRLTLEDRRDYAAAAFMAHDVAVAAPQVDFLLSQK
                     DGPAPLDLLLAAQLAARRQNNVVALDYAQRATTDPRAKPNEGLACAVLVLSITGPKSL
                     PYSQAWERIAKLARDSSNPASLDALVFLAQQQSRAPSPLTGEDTSFSLHARITSPPAT
                     TMGPLEVANALEKHPNARPYHQLLALGLRAQHDPALANQYVADAVERFGGGDDETLVS
                     LAAWLNSVGRADKTLQALPLERATRRQDLYLRYVDALAALEHWSEVKELLSLERFPLE
                     PVFQHMYLAIARTRLGEATAATNEWQRALEAANTLEKLLVLAGYAEQNKANDVADRAY
                     SE"
     gene            complement(1524..2111)
                     /locus_tag="VE486_04660"
     CDS             complement(1524..2111)
                     /locus_tag="VE486_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38410.1"
                     /translation="MTSQVPPGAVLTTIITNYNPLNYDTVSPGVPLNVAVGDPDPNLV
                     ALGLSASLLNDPANFDIPLPSLGLFQDMSISFAVNVAGNGFRTAALWYSVNGGVNFTQ
                     APGQIFTLPSSGSLVVSFTVPTAANNAPLLVLRLQFTGGQSSGQNVQDIIDNIQVNGT
                     IIPEPTTIAGGLFGVLGLCWHQRRRLIRFVRLRRA"
     gene            2356..2550
                     /locus_tag="VE486_04665"
     CDS             2356..2550
                     /locus_tag="VE486_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38411.1"
                     /translation="MLANSGIRPAGLVLNRLRRRRGVGYYYYYASHGYGDGEGSYSRN
                     YGQRSGSDHKDNGVSEIGDQ"
     gene            complement(2582..3808)
                     /locus_tag="VE486_04670"
     CDS             complement(2582..3808)
                     /locus_tag="VE486_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HZA38412.1"
                     /translation="MFLLAIVLLTAPAAAPSAFAADATWTGTLSSSWSNLLNWTSLIS
                     PPGSGDIATFSNAGNGNTTLNLGTGVTVRTILFDTSNAAAYTIGSGGVGSQTLTLNNS
                     GAITVNGTVTNNETFNANIVLGTNANASAYTFTNNSTTGGQVLTIAGNVSSGTGGTVG
                     NKTLTLTGAGNGLISGIISGNGTGGTGTVALTKSGAGTWRLSGANTYTGTTTVIGGTL
                     LINGNQSAATGAVTVSNSGTTLGGTGTIGGTVTVNAGANIAPGNGGNTTAILNTGALT
                     LASTSNFRVDINGTIAGSGYDRLNVTTGIVSIGGSNVVVTVGTTLAIGQTFLIVNKVS
                     AGAVSGTFAQGSTVTSGPFSFAIDYAGGDGNDIVLTVTAVPEPSTWIGGAFALAALGY
                     MQRRKLKKFTRERFRS"
     gene            complement(3948..5786)
                     /locus_tag="VE486_04675"
     CDS             complement(3948..5786)
                     /locus_tag="VE486_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38413.1"
                     /translation="MAEDDFMLTPEEARLGRRKRRRIVLITLGVLLLIAAGVFGARPA
                     AGAIKAWQARRHAQRAFAYIEKEKWMDAHGEAVAAYQLRPTEPQALRAVARFLSRTHQ
                     PEALDFWKQLAEKTSLTRQDVRDEAMIAIIASDTARADAAMGELLDSRAEPADWLLAA
                     KLSFQKNLREEAKPYLDKIVTDPRTTESEQFRATLLQLALAGDDQTKRADALTRLKKV
                     AAAKTATSLDALVVLAQNVLSGAANPKPMAGDSAAAAEAALAKEATAATTEGSMGASD
                     LSRALESHPLGKAQHKLLALDLRMHVAPKQREALISRAIADWKGADPSDLASLGAWLN
                     SKGEYQREIDTIPLEKALQSRDLFLQHLDALGALGRWNEIKQLLGSERFPLDPVVQTM
                     YLARCSVQLGEKTAARNNWQRALEAAADNPGKLMTLADYAEKNGNADIAEAAYHGTAA
                     MSPKLRVAQDGRLRIAQASHDTKKLHAILAEMLALWPNDPAIQNDEAYTRLLLLPVAG
                     GEEQIDEINAIEQLANKLVQQNPASLPHRTLLALARLKQHRPFAALEVYSNIQIVPNA
                     VSPSALAVHAAVLAANGRRDDARHEIEQAPLDKLLPEEQAGTADLR"
     gene            complement(5878..7623)
                     /locus_tag="VE486_04680"
     CDS             complement(5878..7623)
                     /locus_tag="VE486_04680"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HZA38414.1"
                     /translation="MQNLVTSPTTTDASPVRERHFSTPLALSVLFLAALWFILCVQLS
                     GEWLVNEQYNFGWFVPFFAVYLFWLRWQDRPEPAVRNQKSEIRGNPRALIAGLLAVPA
                     LFLLLPVRLFEIANPDWRLLAWVHTGSVVTLTLLYLWCVGGRAWLRHFAFPVAFIFVA
                     VPWVTPIEGPIIQGLMRAVAAVAAETVTLFGIPAQLEGNLIRVTNGLVGVNEACSGVR
                     SLQTALMIGLLLGELKRLSISRRFALVAGAIALALVANFLRAFFLVWIAATENISEVS
                     RWHGLAGYAIVGLVFVGTLILASLLGRKKEQGRVKIEDWNIQHPTANIAHRISYSALP
                     ASFLVFALCWIAAVELGAHFWYRAHERNLVATSRWDVRWPETAPDFRQLKIDEEVRRV
                     LRFDEGQAAAWMIPAATSRDTLGQSKPNTVACLLYLFRWKPGRNSALLANLHRPDVCL
                     PATGWVQMADNGVRDYPVSASFALPFRHFEFRRGIPGNSTQETAHAFYCLWEDRVAKP
                     SAARSKPQQMSGARSAWTRSERLRAVLEGRRHLGQQLMEFIILSRGPTNLDQIEADFA
                     LALPELITIKNRTDH"
     gene            7677..8579
                     /locus_tag="VE486_04685"
     CDS             7677..8579
                     /locus_tag="VE486_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZA38415.1"
                     /translation="MAVPSSGHLPGAGRLGSPLDTSGRMPKLHCPFGNHMIPTQQILG
                     IKFFNGNVDEAVGLMERHGGYVVAPSGTCFARLREDEPYRRAMLSADLVIADSGLMVL
                     MWRLLRHEKIQRISGLQYLKQLLTNLERERNARVFWILPGTRARQNLLDFFRRKAFPV
                     KIEDCYVAPRYGLEIEGRHLLALVEECRPAHIVIAIGSGPQEKLGHYLRENLSYRPPI
                     HCTGAALGFITGDQITIPDWADRFFLGWFLRLMAQPRRFIPRLIRGFELPWLIARYRE
                     NLPPLRQRAEGRSQRSEVRSQRSE"
     gene            complement(8663..>8928)
                     /locus_tag="VE486_04690"
     CDS             complement(8663..>8928)
                     /locus_tag="VE486_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38416.1"
                     /translation="NALCMSLLLSLDCIRDRLEGGALFITSGSSVVRLQPARLPQPQE
                     VNAEAFHVSRDQLSAHQLGMRSNKKIWRRHGGTRPARDFRRAL"
BASE COUNT         1918 a         2498 c         2620 g         1892 t
ORIGIN      
        1 ctcggagtaa gcccggtcgg cgacatcgtt ggctttgttc tgctcggcgt aacctgcgag
       61 cacgagtaat ttctccagag tgttggcggc ttccagggca cgctgccatt cgttcgttgc
      121 agccgttgct tctccgaggc gggttcgcgc gatggccagg tacatatgtt ggaaaaccgg
      181 ctcgagcggg aaacgttcaa ggctcaatag ctccttcact tcggaccagt gttcgagcgc
      241 ggccagcgca tcaacgtatc gcaaataaag atcttggcgc cgcgtcgcac gctccagcgg
      301 cagcgcctgt aaggttttgt ccgcgcgacc cacgctgttg agccacgcgg ccagcgaaac
      361 gagagtttca tcgtcgccac cgccaaaccg ttcgactgcg tccgcgacat attgatttgc
      421 cagcgccgga tcgtgctggg cgcgaagtcc aagcgccagc aactgatggt aaggacgcgc
      481 attgggatgt ttctccagag cattcgccac ttcgagcggt cccattgtgg ttgcgggcgg
      541 cgaagtgatt ctggcatgaa gagagaaaga agtgtcttcg ccagttaagg gcgacggcgc
      601 tcgcgactgc tgctgggcga gaaaaaccag cgcgtcgaga gacgccggat tgctcgaatc
      661 ccgcgccagt ttcgcgatgc gctcccatgc ctgagaataa ggaagcgact ttggcccggt
      721 gatggagaga acgagaacag cacacgccag accttcattg ggcttggcgc gcggatcggt
      781 tgttgcccgc tgtgcataat ccagagcaac aacattattt tggcgtcggg cagcaagctg
      841 agcggcaagc aggagatcta atggcgcagg cccatccttt tgcgacaaaa ggaaatcgac
      901 ttgcggcgcc gcaactgcca catcatgggc cataaacgca gccgcagcat agtcgcggcg
      961 atcttcgagc gtaaggcgat gtgcttcatc cactttcttc caccactcga gcgcggctgt
     1021 ccattggccc gatcgggacg ctacccgcgc aatggcacgc cacgagtcag gctcgctcgg
     1081 gcgcagcagg taggcgtcgc gtgctttggc actggcctcg ctccatttct tttggttgac
     1141 tagcgcgaac gcctcctggg cgaggcgacg cgattgccag cctttgattg ctccagcaac
     1201 tgccggcgcg gcaaagtaga gtgcggcgat gagagcaatt gccgcgggaa ccgccagaaa
     1261 aagtagcagc cggaggcgtc gcgctcggcg ttcttccggc ggaatcaggt cgagatcatt
     1321 tttggccgga tgagggcgtg atggctgcct gcgcacgagg ggagacgttt actatggcgt
     1381 ggtcgcatat gcaacgcgat gccgcctgcg ttagaatctt caattctgca gggcaccgct
     1441 tcccgaagga aagattgttt cggcgcgacg ccgaagccag cacgcgagat gcgcgctacc
     1501 cgaaaaccgc gaatccgcgc gcttcaggca cgacgcaaac gcacaaagcg aatcaatcgc
     1561 cggcgctgat gccagcagag cccaagaacg ccgaatagcc cacccgcgat tgtcgtgggc
     1621 tcgggaatta tcgtgccgtt tacctggatg ttatcgataa tgtcctggac attttggcca
     1681 cttgactgac ccccggtgaa ttgcagtcgc aggacgagta atggcgcgtt gttagcagcg
     1741 gtagggacgg tgaaacttac aactaatgac ccggagcttg gaagtgtaaa gatttggcca
     1801 ggggcctggg tgaaatttac ccctccattg acgctgtacc acagcgctgc ggtacggaag
     1861 ccgtttccgg cgacattaac cgcgaagctg atggacatgt cctgaaacag acccagcgag
     1921 gggagaggga tatcaaaatt agccgggtca ttcagcaagc tcgcgctaag accgagcgca
     1981 acaaggttcg ggtccgggtc gccaacagca acatttaaag gtaccccggg agaaacggtg
     2041 tcgtaattga ggggattgta attcgtaatg atcgtcgtca gcacggctcc cggcgggacc
     2101 tgggacgtca agttaaccgc cgggcctggg ggaacccctt cgaaattaaa ataagctatt
     2161 aaatctgccc tcgcgctcgg ggcaaaaccg gccatcagca tcaacgcgcc gatcacaatt
     2221 aaaagcgaac gaaacggtaa agcgggcggc cgaaatttct taaattttcg catatttcct
     2281 gccaaggctc gaagattgcg ctttcttgta acagttcacg ggaaacaggt cacggaaaaa
     2341 cgccgcgcaa tttctttgtt agcgaactca gggattcgac cggccggtct ggtgctcaat
     2401 cgattgcgcc ggaggcgcgg cgttggctat tattactatt atgcctcgca tggctacggc
     2461 gacggtgaag ggtcgtattc gcgcaactac ggacaacgat ccgggtccga tcacaaagat
     2521 aacggcgtat cagagatcgg tgaccagtga tcggtgatga gagatcaatc gtctcttggt
     2581 atcaactccg aaagcgttcg cgagtgaact tcttcagttt ccgcctctgc atgtagccga
     2641 gcgcagcaag cgcgaacgcg ccaccaatcc atgtgcttgg ctccggcaca gcagtcaccg
     2701 taagcacgat gtcgttgcca tcgcccccgg cgtagtcgat tgcgaaactg aagggcccgg
     2761 aagtgactgt actgccttgc gcaaatgtgc cgctgaccgc gccggcgctg actttattca
     2821 caatgagaaa tgtctgcccg attgctaacg ttgtgccgac agtaaccaca acattgctgc
     2881 cgccgatact gacaatgcct gtcgtcacgt tgagtcgatc ataaccgctg ccagcgattg
     2941 tcccattgat atccacgcga aaattggatg tggatgccag tgtgagcgcg ccggtgttga
     3001 gaattgcggt cgtatttccg ccgttcccag gcgcaatatt tgctcctgca ttaacagtga
     3061 cagtccctcc gattgtaccg gttccgccaa gcgtagtgcc gctattgcta acggtgacgg
     3121 cacccgtcgc cgccgactga ttcccattaa tcaaaagcgt accgccgatg accgtcgtcg
     3181 tccctgtgta ggtattcgcg cccgacagtc tccacgttcc ggccccgctc tttgtcaacg
     3241 caaccgttcc agtaccgcct gttccgttgc cggaaattat gcctgatatg agtccattgc
     3301 cggcgcctgt tagcgtaagc gttttatttc ctaccgtacc gccggttccc gaactaacgt
     3361 tacctgcgat ggtgagaact tgccctcccg tagtgctgtt gttcgtaaag gtgtacgccg
     3421 aggcgttggc gttggtgccc aaaactatat ttgcgttgaa agtttcattg ttagtgacgg
     3481 tgccattaac ggtgattgcc cctgaattgt tcagcgtgag ggtctggctg ccgacacccc
     3541 cacttccgat ggtgtaggcc gcagcgttcg atgtatcgaa caggattgtc ctaacggtga
     3601 ctcctgtgcc gaggttgagg gtggtattac cattacccgc attactgaat gttgcgatgt
     3661 caccgcttcc gggaggactg atgaggcttg tccaattgag gaggtttgac cagctcgagc
     3721 ttagcgtacc ggtccacgtg gcgtctgccg caaatgcgga aggtgcggca gcgggtgccg
     3781 tcagcagaac gatcgcgagc aagaacatag ccgaaaacga aaagcgcgat cgccgcccag
     3841 ttttgattac attcctcata tgagctcgac gtcgtcccat caggtcagtt tcgcgattcg
     3901 gtgtcaatgt gaaattttgg aatggcgata acgtcgctaa gccatcccta gcggagatct
     3961 gcggtgccgg cttgttcttc gggcagcagc ttgtccagcg gtgcttgctc aatctcgtga
     4021 cgtgcatcgt cacgccggcc gttggctgca aggacggcgg cgtgcacggc gagggcggag
     4081 ggcgagaccg cgttcgggac gatttggatg tttgagtaaa cttcgagcgc cgcgaacgga
     4141 cggtgttgtt tcagacgcgc caacgcgagc agcgtccgat ggggcaagct tgcagggttt
     4201 tgttgcacta atttattggc gagctgttcg atcgcgttta tttcatcgat ttgttcttcg
     4261 ccgccggcca ccggaagtag cagcaggcgc gtataagctt catcgttttg aatggcagga
     4321 tcgtttggcc aaagcgcgag catctcggcg aggattgcgt gcaatttttt cgtgtcgtgg
     4381 ctggcctgcg caatccgcag cctgccatcc tgcgcaacac gtaacttggg cgacatcgcc
     4441 gcagtgccgt gataggcggc ctcggcaata tcggcgtttc cgtttttctc cgcgtaatcg
     4501 gcgagcgtca tcaattttcc cggattgtcg gcggcggcct cgagcgcgcg ttgccaatta
     4561 tttcgcgcgg cggttttttc acccagctgg acgctgcaac gtgcgagata catggtctgc
     4621 acaaccggat cgagcgggaa ccgttcgctc ccgagcagtt gtttgatttc attccagcgg
     4681 ccaagcgcgc cgagcgcatc gagatgttgt aaaaaaaggt cgcgcgattg cagcgctttt
     4741 tcgagcggga tggtgtcgat ttcacgttga tattcgcctt tggaattcaa ccacgcgcct
     4801 agcgaggcga gatcagatgg gtcggcccct ttccaatcgg cgatcgcgcg cgagatcaac
     4861 gcttcacgct gtttcggggc gacatgcatg cgcaaatcaa gcgcgagaag tttatgctgc
     4921 gcttttccaa gcggatgcga ttccagagcc cggctcaaat cgcttgcgcc catgctccct
     4981 tctgtagtgg cggccgtggc ctccttcgcc aaagcagcct cggctgccgc ggctgaatcg
     5041 cccgccatgg gtttgggatt cgcagcccca gagagcacat tctgggcaag aacgacaagc
     5101 gcgtcgagcg atgtagcagt tttcgccgcg gcgacttttt tcaagcgggt cagagcatct
     5161 gcccgcttgg tctggtcgtc gcccgcaagt gcaagctgca acaatgtcgc tcggaactgc
     5221 tcgctttccg tcgtgcgcgg atcggtcacg attttgtcca gataaggttt cgcttcttcg
     5281 cgtagatttt tttgaaatga gagtttggcg gcaagcagcc aatcggccgg ttccgcgcgc
     5341 gaatccagca gctcgcccat cgcggcatcc gcgcgtgccg tgtcgctggc aatgatggca
     5401 atcatcgctt cgtcgcggac atcctgacgt gtgagcgagg tcttctcggc gagctgtttc
     5461 cagaaatcga gcgcctcggg ctgatgcgtg cgtgaaagga aacgcgccac ggcgcgcagc
     5521 gcttgcggtt cggtcggacg caactgatac gccgcaaccg cctcaccatg cgcatccatc
     5581 catttttcct tctcaatgta ggcaaacgct ctttgcgcgt gacgacgcgc ctgccatgcc
     5641 ttgattgcgc ccgccgcggg tcgcgcgcca aaaacgcctg ccgcgatcag aagcaagacg
     5701 cccaatgtga tcaacacgat gcggcggcgt ttgcggcgtc ccaggcgcgc ctcctccggt
     5761 gtcagcatga aatcgtcctc agccatggtt agaagtcgga gtcagagaat cgaaacgcgc
     5821 gttgtcgaga aaacttccgt ggcggaaagt taaattgtcg gcagtcagcg ctagaagtca
     5881 gtggtcagtt ctgttcttga ttgtgatgag ttctggcaaa gcaagcgcaa aatccgcctc
     5941 gatctggtcg agattagtcg ggccgcgact gagaattatg aattccatta attgctggcc
     6001 cagatgccgg cgcccctcga ggaccgcgcg aagacgttcg gaacgcgtcc aggccgaccg
     6061 agcgcccgac atttgctgtg gtttcgaacg ggctgcggac ggtttggcga cccgatcttc
     6121 ccataaacaa tagaacgcat gcgccgtctc ctgcgttgag ttcccgggta tgccacgacg
     6181 aaattcgaaa tgacgaaacg gaagagcgaa tgatgcgctc accggataat cgcgcacccc
     6241 gttatcggcc atctggaccc agccagtcgc tggcagacag acgtccggcc ggtgcagatt
     6301 cgcgagaagc gcgctgtttc ggccgggttt ccagcggaag aggtacaaaa gacaagcaac
     6361 ggtgttcggt ttcgactgac ctaatgtgtc ccgcgaagta gcggcgggta tcatccaggc
     6421 agctgcttgt ccctcatcga aacggagcac ccggcgcact tcttcgtcga tcttcagttg
     6481 gcgaaagtca ggagcggttt ccggccatcg cacgtcccag cgggaggtgg cgacaagatt
     6541 gcgctcatgc gcacgatacc aaaaatgagc gccgagctcg accgctgcga tccagcaaag
     6601 ggcgaaaacc aaaaacgacg cgggaagcgc agagtaggaa attcgatgtg cgatgtttgc
     6661 ggttggatgt tggatgttcc aatcctctat cttcactctc ccttgttcct ttttgcgtcc
     6721 cagcagcgag gccagaatta atgtgccaac aaaaaccagt ccgacgatgg cgtagccggc
     6781 gagcccgtgc cagcgactga cctcggagat attttccgtg gctgcgatcc agacaagaaa
     6841 gaatgcgcgc aaaaaattgg cgacaagcgc gagcgcgatt gcaccagcaa caagcgcgaa
     6901 gcggcgcgaa atcgacaatc gtttcagctc acccaaaagc agaccgatca tcagcgcggt
     6961 ctgcaacgaa cgcacgccgc tgcacgcttc gtttacgccc accagtccgt tggtcacccg
     7021 gatgaggttt ccttccagtt gcgcaggaat tccaaagagc gtgaccgttt ccgcggccac
     7081 ggcggcaacc gcgcgcatca ggccttgaat gatggggcct tcgatcggcg tcacccaggg
     7141 aactgcgacg aaaatgaacg ccaccggaaa cgcgaaatgt cggagccacg ctcttccccc
     7201 cacgcaccag agatacaaga gagtcagcgt gacgaccgac ccagtgtgca cccaggcgag
     7261 cagtcgccaa tcgggattcg caatttcaaa aagacggacc ggcaaaagga gaaaaagcgc
     7321 gggaaccgcg agcaaaccgg cgatcagcgc gcgagggttg cccctgatct ctgacttctg
     7381 atttctgacc gccggctccg gccggtcctg ccagcgaagc caaaaaaggt aaacggcgaa
     7441 aaaaggaacg aaccatccaa aattgtattg ctcgttgacc aaccattcgc cactcagttg
     7501 cacgcacagg atgaaccaga gcgcggctaa gaacaaaacg ctcagcgcga ggggagtcga
     7561 aaaatgcctc tcacgcaccg gcgaagcgtc agttgttgtc ggtgaagtta ccagattttg
     7621 cactgatcga cagcttacca aatttgcgcg caaagcgaaa tcgtaacaca gccatcatgg
     7681 ctgttccgtc gagcgggcat ctgcctggcg caggcaggct gggaagcccg ctcgacactt
     7741 ccggcaggat gcccaaatta cattgtccgt ttggtaatca catgattcca actcaacaga
     7801 ttctcggcat taaattcttc aacgggaatg tggatgaagc ggtcgggttg atggagcggc
     7861 acggcggtta tgtggtggcg ccttcgggaa catgttttgc gcgactccgc gaagacgaac
     7921 cctatcggcg ggccatgctc tcagccgacc tcgtaatcgc ggacagcggt ctgatggttc
     7981 tcatgtggcg attgttgcga cacgaaaaga ttcagcgcat ttctggtctg caatatctga
     8041 aacaactcct cacaaacctg gaacgtgaac ggaacgcgcg cgttttctgg atcctgcctg
     8101 gaacgcgggc acggcaaaac ctgctcgatt ttttccgccg caaagcgttt ccggtcaaga
     8161 tcgaggattg ttatgtcgcg cccaggtacg gattggaaat cgaaggtcgc catcttcttg
     8221 ccttggtaga ggaatgccgg cccgcgcata tcgtgattgc gatcggcagc gggccacagg
     8281 aaaaactcgg acattatttg cgcgaaaatt tgtcttatcg gccgccgatc cattgcactg
     8341 gggcggccct tggcttcatc acaggcgatc agattacgat tcccgattgg gccgaccgct
     8401 tttttctggg ctggtttttg cgattgatgg ctcagccgcg ccgtttcatt ccgcgcctga
     8461 tccgcggatt tgaattgccg tggctgatcg cgcgataccg cgagaattta ccgccgctga
     8521 ggcagagggc agagggcaga agtcagaggt cggaggtcag aagtcagagg tcagagtaac
     8581 gccgttcgga gttccgcctt caggcggtct gctgctcctc gagctcagag atcagatcgc
     8641 agaggtcgga ggtctgccgg tctcaaagcg cgcgacggaa gtctcgcgct ggacgtgttc
     8701 ccccatgccg tcgccagatt tttttattgg agcgcatgcc aagttgatgg gctgagagct
     8761 ggtcacgcga gacgtggaac gcttccgcat ttacttcctg cggttgaggt aatcgagccg
     8821 gttgcaatct gacaacggaa ctcccggagg tgatgaaaag agctcctccc tcgagccgat
     8881 cacgaataca atccaggctc agtaagagtg acatgcaaag agcgttga
//
LOCUS       DASQUQ010000163         5964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_942316, whole genome shotgun sequence.
ACCESSION   DASQUQ010000163 DASQUQ010000000
VERSION     DASQUQ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942316"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VE486_04695"
     CDS             <1..353
                     /locus_tag="VE486_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA38417.1"
                     /translation="AEFYLGLALSDLGEWDRSIEHYERALSLDPKNAKAECNLAGILV
                     GKRMYDEARRRYEHSLQLDPTLFLAHKELGDLLCHAGEYDAAIDQYNQALRLRPNFPE
                     AKDNLAFARSLAGH"
     gene            complement(965..2239)
                     /locus_tag="VE486_04700"
     CDS             complement(965..2239)
                     /locus_tag="VE486_04700"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HZA38418.1"
                     /translation="MKWRNQLLALFCLLAFAGLGVLYFQHWVLRKPFGIILFVGEGLA
                     PSRLAATRAFAGGADNHLSLDSMENLALLTNHSKDFAAPDQAAAVTAIATGVRVTNRA
                     IAINGDGKPIKSLVELAREQGRATGLVTDTKLTDPTSAAFYAHPTDPDDIESIAAEFV
                     NRARLDIAMGGGSAQFLPTAKGGERQDGRDLLLDLRRNGFDIVQTRAELEAIPAWRRS
                     KVFGAFSKTELAFTNEVEERSQQPSLSDMVRRAIQLLQYNTGGYLLVVNAGLMRKAAQ
                     ENNAERTFNQTIELDRAVAVARNYAGPKSTIIVCGDVAIGGLSLNGFPFRQDSGIALL
                     GFNAGGQPWITWATGPNGIKSYGAAKIPGNPNGNGGEQPDVEQREPAAVYTKAALGTV
                     DDVIAFGSGPGTGELRGILDNTKIFEIIRDNL"
     gene            2583..4685
                     /locus_tag="VE486_04705"
     CDS             2583..4685
                     /locus_tag="VE486_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA38419.1"
                     /translation="MKQASRFSPWWVAAICIALAGVTWTVFGQTLQHDFINYDDNLYI
                     YENPDIVSGLTRPAILWAFTHAHAGNWHPLTSLSHMLDCQVFGLNAGGHHFTNVLLHT
                     LAVVLLFLFLQQVTGAPWRSAFVAAVFAVHPLRVESVAWIAERKDVLSGVFFMLTLLA
                     YARYVSGERSLLRYLLVAFFFALGLMAKPMLVTLPLVLLLLDYWPLCRFGSRSSGKDA
                     NFARRSTFFGLVAEKIPLCLLSLLSSLVTWRVQQAFVRSTSEIPLLLRIENAIVSYVR
                     YLWQLLWPADLAPFYPHPPAMPLLVVIAAAVLLGAISAVVLCYGRKRPYLTTGWGWYL
                     IMLLPVIGLVQVGEQALADRYTYLPQIGIALAITWTVADATAGIRQLQRFVAFTAVAV
                     TVTLAWCACHQTRYWRNSESLWLQTLAVTENNDLAHNNLGDVWSKSGRVDDAIQEFQA
                     ALKVTPESPVARNNLGSAFLKQGKVAEAIQEFRRVLSRDPQSITGRFNLAGALLRSGR
                     ATEAVAEYEKVLAIKPDFAQGHLALGHALMRAWRLADAIAQFKIAVQLQPGHAHARNS
                     LATAFAAKQQWGEAIRCWRETLKINPNDVAAQSGLAWTLATAPDPVLRNGAEALAISQ
                     HLSKTTDASNPMLLRVLAAAYAEAGRFPEAIETTERGIALATSQHRADLAALLQGDLK
                     LLQSAQPLRDAGKSPITR"
     gene            5119..>5964
                     /gene="gpmI"
                     /locus_tag="VE486_04710"
     CDS             5119..>5964
                     /gene="gpmI"
                     /locus_tag="VE486_04710"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZA38420.1"
                     /translation="MAGPVMLIIRDGWGINPGGREKREENADATLLARTPFHDQLYRD
                     YPGGKLSASGADVGLPDGQMGNSEVGHLNLGAGRIVHQDLTRINKAIVDGELARNPIL
                     RETFARARDHRLHLLGLVSDGGVHSHYSHMIALANAAREAGVAEIFVHAFTDGRDTSP
                     TGGAGYLKTCEEELRKSGAQTVTVVGRYFAMDRDRRWDRTKKAWDAIVLGRGEISKSS
                     PSAAVERQYSLGKTDEFMPPLIFSYANEQRVRDGDAVLFFNFRADRARQLSQAFLLKD
                     FDGFDR"
BASE COUNT         1225 a         1727 c         1658 g         1354 t
ORIGIN      
        1 aagccgagtt ttatctcggc ctcgccctca gcgaccttgg ggagtgggat cggtccatcg
       61 aacactacga gcgcgcgctt tcgctggatc ctaagaacgc gaaagccgag tgtaacctcg
      121 ctgggatatt ggtcggcaag cgtatgtacg atgaggcccg tcgccgatac gaacacagtc
      181 tccaactcga tcccactttg tttcttgcgc ataaggaact gggggatttg ctctgccatg
      241 caggcgagta tgacgcagcg attgaccagt acaaccaagc gttgcggctt cgccccaatt
      301 tccccgaggc gaaggataat ctggccttcg cgcgttctct cgccggccac tgatgatgtg
      361 ggaatgaaga acgcttggtg ccggcgtgca gacggtgggc tattgtttat ctggatcgga
      421 gcatttatga gagttcgctt gcagacaccc aaaataagct gaaacatttg gccgctggcg
      481 gggtctaatt actagaaagc ctaattacca tctatgaatc taaaaccttt catttcagta
      541 aattgctgct tgctgcgttt ggccatcgtt ccaatcatcg cgttgagttt ggtggcggca
      601 agcatcggaa cagtaaatgc cgatccccag aaccggagag tcacatacgt aaagaaatac
      661 gtgaggattg tcggctccaa tattcccgtc ccggtttggg tagcggaagg tccgctacca
      721 aaaacaacgt atcccgtccg gagttattcg gaggacgagt tcaggaaaat tcccggtgct
      781 ataactccag ctcaggttct ggccaacgac ccgaatatca cgataagcgg tagacactaa
      841 tcggtcgcgg agccaggcca acgacgggta gcgcgcaatc attacgcgac caaccagccg
      901 tccctctgtg agcttggcgc gtgtctttgg attctttgcg caacggccag agacgggcca
      961 cagtttaaag attatcgcgg atgatctcaa agatcttcgt attatcgagg ataccgcgga
     1021 gctcgcctgt gccgggaccg ctgccaaacg caatgacgtc atcgaccgtg ccgagagccg
     1081 ctttggtgta gacggcagcc ggctcgcgct gttccacgtc cggttgctca cctccattgc
     1141 cgttcggatt gccgggtatc tttgctgctc cgtaggattt gatgccattc ggacctgtcg
     1201 cccaggttat ccacggctgc ccccctgcat tgaacccgag cagggcaatg ccgctgtcct
     1261 ggcgaaatgg aaatccattt aagctcaatc cgccgattgc gacatcgccg cagacaatga
     1321 ttgtcgattt tggcccggca taattccgcg ccacggcaac tgctcggtct aattcgatgg
     1381 tttggttgaa tgttctttcc gcgttgtttt cttgcgcggc tttgcgcatc agtcccgcat
     1441 tgacgacgag caagtacccg ccagtgttgt actgaagtaa ttggatcgcc cgccgcacca
     1501 tgtccgagag acttggttgc tggcttcgct cttcaacttc gttggtgaag gcaagctccg
     1561 tcttgctaaa cgcgccgaaa actttcgacc ggcgccatgc gggaatcgcc tccagttcag
     1621 cccgcgtttg aacgatatcg aacccattgc gacggagatc gagcaaaaga tcgcgcccat
     1681 cctgtctttc tccgcccttt gcggtgggaa gaaattgtgc cgacccgcca cccatcgcga
     1741 tgtcaagtct tgcccgattc acgaactcag ccgcgatgct ctcgatgtca tcgggatccg
     1801 tcggatgcgc gtaaaaagcc gcgctggttg gatcagttag ttttgtatcc gtcaccagcc
     1861 cagtggcccg tccttgctcc cgtgccaatt caacaaggct cttaattggt ttcccgtccc
     1921 cattgatcgc gatagcgcga ttagttactc tcactccagt ggcaattgca gttacagcag
     1981 ccgcctgatc cggagcggcg aaatccttcg agtgattcgt cagcaaagcg agattctcca
     2041 tggaatccaa gctcagatga ttgtctgccc cgccggcaaa agctcgcgtc gccgcaaggc
     2101 gactaggcgc aagcccctcg ccgacaaaca agatgattcc gaaaggcttc cggaggaccc
     2161 agtgttgaaa ataaagaaca ccgagcccgg caaaggcgag caaacagaaa agggccagca
     2221 attgattgcg ccatttcatt tcctgggaat gtagcgcatg cgccccccgc ttgcggtggg
     2281 aaaatcgcaa gtgacacgca tgcgccacca tccgcatcct gccgaagctg gtgtgcgcgg
     2341 cgacgtgctc cgactgcccg cgacaagagc atcgcaacca ctacgacgga gacggcgcgt
     2401 ttggcaaaaa atttcgtgta tcagcagcca tggaatgtaa acatgctgcg atgttgcgcg
     2461 gtcaaaaaat cgtcgatcgg ccgaggcagt cctttcttcc atttgcctcg gaaccaccgc
     2521 gccgctgtcc aaattacctt ctggtgtatc aacgcaatct ttagggtgtg gctcgagcgg
     2581 gtatgaaaca agcatcgcgc ttctctccct ggtgggtggc ggcaatttgc atcgccttgg
     2641 cgggcgttac ctggaccgtc ttcgggcaaa cgctgcaaca tgacttcatc aattacgacg
     2701 acaatctcta catttatgaa aaccccgata tcgtttctgg cctgacacgc ccggcgattc
     2761 tttgggcgtt tactcacgct catgcgggca actggcaccc tctcacttcc ctttcccaca
     2821 tgcttgattg ccaggtcttc gggctcaacg ctggcggcca tcatttcacg aatgtgcttc
     2881 tgcacacact cgccgtggtt ttgcttttcc tgttcctgca acaagtcacc ggcgcgccct
     2941 ggcgcagcgc ctttgtcgcg gccgtgttcg ccgtacatcc gttgcgggtg gaatcggtgg
     3001 catggattgc ggaacgcaag gacgtgctca gcggtgtctt cttcatgctt accctgctcg
     3061 cttacgcccg ttatgtctcg ggcgaacgtt ccctccttcg atatttgttg gtcgcgtttt
     3121 tcttcgctct tgggctgatg gcaaaaccga tgctggtgac cctgcctttg gttctgttgc
     3181 tgcttgatta ctggcccctc tgccgcttcg gttcccgctc gtcgggaaag gacgcaaact
     3241 tcgcgcgtcg ctccaccttt tttggcctcg tcgcagaaaa gattcctctc tgtctattga
     3301 gcctcctctc ctcgctcgtc acctggcggg ttcagcaggc atttgtgcga tcgaccagcg
     3361 agatcccgct gctgctgcgt attgagaacg ccatcgtcag ttacgttcgc tacctttggc
     3421 aattgctctg gccggccgat ctggctccgt tttatcccca cccgccggca atgccactgc
     3481 tggtggtaat cgcggctgct gttctgctcg gcgccatttc ggcggtagtc ttatgttatg
     3541 gacgaaaacg tccatatctg accacgggct ggggttggta ccttatcatg ctcttgccgg
     3601 taatcggctt ggtgcaggtg ggagagcaag cgctggcaga ccgttatacc tacctgccgc
     3661 aaattgggat tgctcttgcg atcacgtgga cagtcgccga cgcaaccgct ggcattcggc
     3721 aattgcaacg cttcgtggcc ttcaccgccg tcgcggtaac ggtcactctc gcgtggtgcg
     3781 cttgccatca aacccgctac tggcgaaaca gcgaatcgct ttggctgcaa acgctggccg
     3841 tgacagagaa caacgacctg gcgcacaaca atctgggcga cgtgtggtcg aagagtggac
     3901 gtgtcgatga cgccatccaa gagttccaag ccgcgctgaa agttacgccg gagtctcccg
     3961 tcgctcgcaa taatctggga tcggcctttc tcaaacaagg aaaagtcgcc gaagcgattc
     4021 aggaattcag gcgcgttctt tcccgcgacc cgcaaagcat cacaggccgc tttaatctcg
     4081 ccggcgcgct cctgaggagt ggtcgcgcca ccgaagcagt cgctgaatat gaaaaggtcc
     4141 tggcgatcaa gccggatttt gcgcagggcc atttggcttt gggacacgcc cttatgcgcg
     4201 cctggcgtct cgcagacgca atcgcacaat tcaaaattgc ggtgcagttg cagcctggcc
     4261 atgcccatgc gcgtaacagc ctcgcgaccg cctttgccgc gaagcagcag tggggcgaag
     4321 cgatccgctg ttggcgagag actttgaaaa tcaatcccaa tgacgttgcc gcccaatctg
     4381 gtttggcatg gacactggcg acagctccag atcctgtgct gcggaatggc gccgaagctc
     4441 tcgcaatctc acaacacctc tccaaaacca ctgacgcgtc gaacccgatg cttcttcggg
     4501 ttctcgccgc cgcctacgcc gaggcagggc ggtttcccga agcgatcgaa acgaccgagc
     4561 gtggaatcgc gctcgcaact tcgcaacacc gcgccgacct cgctgctctc ttgcaaggtg
     4621 acctgaaact cttgcaatcg gcgcagccgt tgcgcgatgc cggcaaatcg ccgattactc
     4681 gttaggggaa acaggaaaag gcggagtttg ctcaaagctg gcgcgcaggg gtcatgcgcg
     4741 tgagcggttc tgatgattcg atgagccaaa gctcgcgcga ccagccccgc cgcgaatctc
     4801 tcgcagttat acactcggcg aattgtaacc gcgagtgaca acgaatccgc ttaccgcatc
     4861 gtccaacgat tgcgcgtggc cgcgacttgg cctggcggct gtgttgggtc aaagaagaaa
     4921 attcccggaa tcgtcactat tgaggatttg atctaacgcc tcgtttcttc gagcgaaggg
     4981 cgaacttaga aagcaatgag gttgctttga atttcgcggg gcccagcttg cagtgagcgg
     5041 tcgcaagcga gacgtatgcg ctaccatacc gaaagaaccg cttgtcgatc ttggtaatcg
     5101 tcagcaaagt gcccgcgtat ggctggtccg gttatgctta ttattcgcga tggctggggc
     5161 attaatccgg gcggtcgaga gaaacgggaa gaaaatgcgg acgcaacact cctcgcgcgg
     5221 acgccgtttc acgaccagct ctaccgggat tatccaggcg gtaagttgag cgcgagcggg
     5281 gctgacgtcg gtttgccgga tggacaaatg ggcaattcgg aagtcggcca tcttaaccta
     5341 ggagccggca ggatcgtcca tcaggacctg acgcggatta acaaggccat cgtggatggc
     5401 gaactggccc gcaacccaat actgcgggaa accttcgccc gcgcgagaga tcatcggctg
     5461 catctgctcg ggctcgtctc cgatggcggt gttcacagcc attactcgca catgatcgcg
     5521 cttgcgaatg cggcccggga agcaggcgtc gcggagattt tcgtccatgc tttcaccgat
     5581 ggacgcgata catcgccgac tggcggcgct gggtatttga aaacctgtga ggaagaactt
     5641 cggaaaagcg gcgcccagac cgtcacagtg gtgggacgtt atttcgcgat ggatcgcgat
     5701 cgtcgctggg accgcacgaa aaaggcctgg gatgcaatcg tcctcggtcg cggcgagatt
     5761 tcgaaatcgt cgccttctgc cgcggtcgaa cgacaatatt cgcttggcaa gaccgatgag
     5821 ttcatgccgc cgctgatttt ttcttacgcg aatgagcagc gcgtccgcga cggcgacgcc
     5881 gttttgtttt ttaatttccg cgccgatcgt gcgcggcaac tatcacaggc ctttttgctc
     5941 aaggacttcg atggtttcga tcgc
//
LOCUS       DASQUQ010000164         5137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_684524, whole genome shotgun sequence.
ACCESSION   DASQUQ010000164 DASQUQ010000000
VERSION     DASQUQ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684524"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2491
                     /gene="dnaE"
                     /locus_tag="VE486_04715"
     CDS             <1..2491
                     /gene="dnaE"
                     /locus_tag="VE486_04715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZA38421.1"
                     /translation="NTSRLAERLTFSLENIGYEFPEYPVPAGHSMDSFLRTIVWFGAQ
                     QRYAAISTAVKRQLEEELALISKLGFPGYFLIVWDIVNFCREHNIMVQGRGSAANSAV
                     CYCLGITPVDPVSNHLVFERFLNESRKGWPDIDLDLPSGDRREAVIQEIYRRYGKHGA
                     AMTANVISYRGRSAAREIGKALNFSPSILDRFSHLFANGDFPHTLDLESQIEQAGLSR
                     EHPRMPGFIRLYQSIYGLPRHLGQHSGGMIICQNKLSSFVPLENASMPGRVVAQWDKD
                     DCEDLGIVKVDLLGLGMMSVMQDTFELCRERDRPDRPGLDLAHIPKDDPATFDIMQKA
                     DTIGVFQIESRAQMATLPRMKPKCFYDVVIEVAIIRPGPIQGDMVHPYLARRAGKEPV
                     TYFDDRLKPVLQRTLGVPLFQEQMLKIAMVMADFSGDEAEELRRALSFHRSEERMNKV
                     CAKLRAAMERKGVTPDKIDKIIQSISSFALYGFPESHAISFAILAYGSAYLKVHRAPE
                     FYASLLNNQPMGFYTPATIVKDAQRHGLKIKPVCISQSNWRCTVVDDNTVRLGFCVVN
                     GLQQEHAEELMRQREDRPFESLDDFKQRVSLPKDELRTLAELGALNCFAEHRRAAMWE
                     VEETMHDDLLGSVRVSRVGFGVAPKQSFEEFMTRDGFGAFRKVRDGEEALANTRDACA
                     TQTPRSPLAQMTLFERVKADYETMNLTTGPHPMKLLRGSLLHIWRASDLAQARHGATV
                     RIAGNVICRQRPGTAKGFVFISLEDETGVSNAIVDPDLFERFRLAITEEAFLLIEGQV
                     QNSDGVVLIKAREIRALAHEQLIGSESHDFH"
     gene            complement(2966..3196)
                     /locus_tag="VE486_04720"
     CDS             complement(2966..3196)
                     /locus_tag="VE486_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38422.1"
                     /translation="MENVTADLAQALRERLAIIADEESRRDAETHMKRLNAISEKIDK
                     LQAALPRPIDPQLAHYLQRRSYDKALEFLEQL"
     gene            3736..4167
                     /locus_tag="VE486_04725"
     CDS             3736..4167
                     /locus_tag="VE486_04725"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZA38423.1"
                     /translation="MKHFVFRWAITTIAVMVASSIIHGIRYDTVAALIGAALLLGILN
                     AFVRPILLILSAPVILLTLGFFILVLNGLMLLFVPSIVIGFHVDSFWSAFWGAIIISI
                     VSWILSAFFRGSDGHVHVLTHHTQIRRVRGRVIEHPEGDRK"
     gene            complement(4262..4864)
                     /locus_tag="VE486_04730"
     CDS             complement(4262..4864)
                     /locus_tag="VE486_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZA38424.1"
                     /translation="MSGKDPENQRRSEEDAEDVRLMRLVARGDPSAFENLIERHQALV
                     AGTVARMLGSNSDVEDIAQQVFIRVWKSARRYVPRAKFTTWLLKITRNLVFNEMRRAR
                     RRAQVPLQSEPGAEEIPLKDETNPAPDASLLEDELKRAIEEAILQLPESQRMALILRR
                     YEQLSYEQIAEVLDLSVPAVKSVLFRARTELRSRLSKYLG"
BASE COUNT         1087 a         1331 c         1452 g         1267 t
ORIGIN      
        1 caacacgtcg cgcctggctg agcggctcac gttttccctg gaaaacatcg gttatgaatt
       61 tccggaatat ccggtgccag ctgggcactc aatggattca tttttgcgga ctatcgtctg
      121 gttcggcgcg caacagcgtt atgccgcgat cagcacggca gtgaaacggc agctcgaaga
      181 agaactcgcg ctcatcagca agctcgggtt cccaggatat tttttaattg tctgggacat
      241 agtgaatttt tgccgtgagc acaacatcat ggtgcaggga cgtggcagcg cagcaaacag
      301 cgccgtttgt tattgccttg gcatcacgcc ggtcgatccg gtgagcaatc atttggtctt
      361 cgagcggttt ttaaacgaga gccggaaagg gtggccggat attgatctag atcttcccag
      421 tggcgaccgg cgcgaggccg tcattcagga aatttatcgc cgttacggca agcatggcgc
      481 ggccatgacg gcaaacgtga tcagttatcg cggacgcagc gccgcgcgcg aaattggcaa
      541 agcgttaaat ttttcaccga gcattttgga tcgcttctca catttgtttg cgaatggcga
      601 ttttccgcac acgctcgatc tcgaatcgca aatcgagcag gcgggtttgt ccagggaaca
      661 tccgcggatg ccgggattca tcagacttta tcaatccatc tacggtttgc cgcgacatct
      721 tggccagcat tccggcggaa tgattatttg ccaaaacaaa ctgagctcgt ttgtgccgct
      781 ggaaaacgcg tcgatgccgg ggcgggttgt cgcccaatgg gacaaggacg attgcgaaga
      841 tctcgggatc gtgaaagtcg atttgcttgg cctcgggatg atgtcggtta tgcaagatac
      901 gtttgaattg tgccgggaac gggaccgtcc cgacaggccg ggactcgatc ttgcccacat
      961 tccaaaagac gatcctgcga ctttcgacat tatgcagaag gccgacacga tcggcgtatt
     1021 tcaaatcgag agtcgtgccc aaatggcgac cttgccgcgg atgaagccga aatgttttta
     1081 cgatgttgtg attgaagttg cgatcatccg gccaggaccg attcagggcg acatggtgca
     1141 cccttatctg gcgcgccgcg ctgggaagga gccggtgact tatttcgatg atcgattgaa
     1201 gccagtgctc caacgcacgc tcggcgtacc gctgtttcaa gaacaaatgc tgaagatcgc
     1261 gatggtaatg gccgattttt ccggcgatga ggcggaggaa ttgcggcgcg ccctaagttt
     1321 ccatcgctcc gaggaacgga tgaacaaagt ttgcgcgaaa ttgcgcgcgg cgatggaacg
     1381 caaaggtgtg acgcctgaca agatcgacaa aatcattcag tccatctcat cgtttgcgct
     1441 ttacggattt ccggaatcgc acgcgatcag tttcgccatt ctggcgtatg gcagtgccta
     1501 tttaaaagtg catcgcgccc ctgaatttta tgcgagcctg ttaaacaatc agccgatggg
     1561 attttatact ccggcgacga tcgtgaagga tgcacagcga catgggctca agatcaaacc
     1621 ggtgtgcatc tcgcaatcta attggcgttg cacagttgtg gatgacaaca cagtgcgttt
     1681 gggcttttgc gtggtaaacg gattacagca agaacacgcc gaggaactca tgcgccaacg
     1741 cgaagatcga ccattcgagt cgctcgacga tttcaagcag cgggtctcgc ttccaaagga
     1801 tgaattgcgc acattggcgg aactcggagc gctcaattgt ttcgctgagc atcggcgcgc
     1861 ggcgatgtgg gaagtggaag agacgatgca tgatgatttg ctgggtagcg tacgcgtctc
     1921 gcgtgttggt ttcggcgtcg cgccgaaaca atctttcgaa gaattcatga ctcgcgacgg
     1981 atttggcgcg ttcagaaaag ttcgcgatgg cgaggaagcc ctcgccaaca cgcgagacgc
     2041 gtgcgctacc cagacgccgc gatcaccgct tgcgcaaatg acgttatttg aacgggtgaa
     2101 agccgattat gaaacgatga acttaaccac cggtccgcat ccgatgaaac ttttgcgcgg
     2161 tagtttgctg cacatttggc gggcgagcga tcttgcccaa gcgcgacatg gcgcaactgt
     2221 acggatcgcc gggaatgtga tttgccgaca gcgtcccggc acggcaaaag ggtttgtttt
     2281 tatcagtttg gaagacgaga ccggtgtctc caatgcgatt gtcgatcctg acttgttcga
     2341 acggttccgg ctcgcgatta ccgaagaagc atttctgctg atcgaaggcc aagtgcagaa
     2401 ttcggatggc gttgttttga ttaaagcgcg cgagatcaga gcgctcgcgc acgaacagct
     2461 tatcggaagc gagtcgcacg attttcatta atatctctaa ccacggatta cgctgatcat
     2521 acggatcggc tcagactgac attgcttccg gcgctgctcg ttgctttcga ggatttgact
     2581 ctgcgtggcg taatagggct gattaagaaa taggcgagtg cgccgaaatc tcgcccgcaa
     2641 attttgcatt ccgagtagtc ctgcaactcg acgttgattt tcgtgtaatc cacgccggca
     2701 tcttcaagcg ctcgtttcca acccctgaac ggccggacgg cgtaacccgc agatcaacat
     2761 cgggcgactc cgtttgacga cggaaatatt aagcgggccc acagcacgcg tggagccgtt
     2821 ggatttcacg gataaggacc tccgcgctgg gagcaatgcc atggaaaaaa tggtatggaa
     2881 cccgcgcgga ggcgtcgaac ggattcaaag acctgcccac gtcactactg gcggccgagc
     2941 agttcgcgga tcggcacaag tgccgctaca gttgttccag gaattcgagc gctttgtcgt
     3001 agctgcggcg ctgaagataa tgcgccagtt gcggatcgat tgggcgtggc agcgctgctt
     3061 gcaatttgtc gatcttttcc gaaatagcgt tcagccgctt catatgcgtt tctgcatcgc
     3121 gccggctttc ttcatcggca atgatggcga gacgttctcg cagagcctga gcgagatcgg
     3181 cagtcacatt ttccattgga ttagccacta ctgagcacaa atcgacacga ataattgggg
     3241 aaatgttttg tgatagtgtc cattcgtgct tacacgttgc tcggcttagc ggcgaccggc
     3301 ttccgagagg agttgtcgcc tggcttcgag ccagtccgaa tccgcgtcgc ctgggagggc
     3361 aaacctgcgg cggcgttccg aaatgaaata cgcccgcaga cgaatttcct catgggatgg
     3421 tcccgctgtt gttcccgcat cgggaggtct cgtgacttcg gctttttgcg caggggcact
     3481 ctttttgcga cgtggtaggg cgctctttcg actcgttgtt ggaggccgcg cacgagacgt
     3541 tttgccgggc tttacttcgg taggcgatcc gccggcttcc ggtgtcgttt gctttttatt
     3601 ttggacaatt ttgttctttt tcggcacaga ttgatcggtg ttgattattt caatccgttt
     3661 actgcgcatc ggccgcttcg caagctgttt ctcgcaggtt cccagcggag tgataaatta
     3721 caaaaacttc cgaagatgaa gcattttgtt ttccgctggg ccattacgac aattgcggta
     3781 atggtcgcct cgtcgataat ccacgggatc cgctacgata cggtcgccgc cctgatcggt
     3841 gcagcccttc tgcttggcat tctcaatgca tttgttcgtc cgattctatt gatcctcagt
     3901 gcgcccgtaa tcctgctgac gctcggcttt ttcattctcg tcctaaatgg tctgatgctg
     3961 ctcttcgtgc ccagtattgt gatcggattc cacgtggaca gtttttggag cgccttctgg
     4021 ggcgcgatca tcatcagcat tgtaagctgg attctcagtg cgttttttcg tgggagcgac
     4081 gggcacgtcc atgtcctcac ccatcacacc cagattcggc gtgtccgggg ccgtgtgatt
     4141 gagcaccccg agggcgaccg aaaatgacgt agtccgtgga aacgctcaac atcgaacatc
     4201 gaatgcagaa gattctgcat tgggcgttgg atgttgggcg ttcgatattt ctgatgtttc
     4261 gttaaccaag atatttgctc agtcgcgagc gcagttctgt tcgcgcacgg aagagaacgc
     4321 tttttaccgc cggcaccgag agatcgagaa cctcggcaat ttgctcgtag ctgagctgct
     4381 catatctccg caatatcaga gccatgcgct ggctttctgg aagttggaga atcgcctctt
     4441 ctattgctcg cttgagctca tcctccaaaa gtgaagcgtc cggagccggg ttcgtttcat
     4501 ccttcagcgg gatttcctcc gcgcctggct ctgattgaag tggaacctgc gcacggcgcc
     4561 tggcgcggcg catctcgttg aagaccaggt tacgcgtaat ttttagcaac caggtggtga
     4621 atttggcgcg cggaacatat cggcgagcgc ttttccagac tcgaataaaa acctgctgcg
     4681 cgatatcttc cacgtccgag ttgctaccca gcatgcgcgc gaccgtgccg gctacgagcg
     4741 cttgatgccg ctcgatgagg ttctcgaatg cgctggggtc gccccgggcc accagccgca
     4801 tcagccgcac gtcttccgcg tcttcctccg atctcctctg gttttctgga tctttgccag
     4861 acatcggtca cttagacggt tgcaccgggc ggaattttgc taaaagaatg ggaaatggcg
     4921 aaaaactggc tctgtgccac attcgtcgcc atggtttttg ttgcaagctg gaagcgctct
     4981 gtgcatgctt gcttgccgag ggcattgccc ggcctatgaa ttacctgctt atcccaaaag
     5041 aactcagatc gatcgctgaa aaagttgagg cgcggcagcg gatttcggag gctgatgctc
     5101 ttactctcta tcgttcaaac gatcttaacg cgcttgg
//
LOCUS       DASQUQ010000165         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_34241, whole genome shotgun sequence.
ACCESSION   DASQUQ010000165 DASQUQ010000000
VERSION     DASQUQ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34241"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..847
                     /locus_tag="VE486_04735"
     CDS             218..847
                     /locus_tag="VE486_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38425.1"
                     /translation="MTTVFWIGEKPSENNPVPNRTSSWDKNWTKNYGGVDDPNPANRS
                     NYIPAKFTPRQNPFYCALPYNDKAREGHRPEAPRVVPWFNNAYQGPAVSTCKGRWVAI
                     RKGNRTVYAQWEDAGPFRTDHWQYVFGNERPKPNLNKGAGLDVSPAVRDYLGMNDTDV
                     TDWRFVEFSEVPRGPWSTHGENNTFVVNDRKKGVKLAEASKPGGGPIVR"
     gene            1091..2416
                     /locus_tag="VE486_04740"
     CDS             1091..2416
                     /locus_tag="VE486_04740"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HZA38426.1"
                     /translation="MGNRCAVRLLYCAIFIACNLRVAAQTETASENANLLTGKAAMGD
                     WTADAPGVRRRITVKDLPAPSSNVFAINRPGVVERPEGAQPQVPPGFKIEMYANGFRD
                     PRYLRTAPNGDIFVADSRGANRIRVLRDTKGSGKPDVTETFTEDDLNKPFGMAFYPPG
                     NDPQFLYVANTDGVIRFPYRNGDLKARGPAEKLSAHLSGGAGNLRSGGHWTRDIVFSP
                     DGKKMYVSIGSRSNVSDNAAEADRARIFEFNPDGSGRKVYAWGVRNAVGIAFRPGSDE
                     LWMSTNERDEIGEDLPPDYISSVRPGGFYGWPWFYIGNHVDPRHKGKHPELADKVIVP
                     DVLVEAHSATLSLCFYTGDQFPAEYKGDIFAAFHGSWNRKKRTGYKIVRVPFDDGKAL
                     GEYEDFVTGFVTPDGQVWGRPVGITVAKDGSLLFSEDGNGTIWRVSYGK"
     gene            complement(2487..2804)
                     /locus_tag="VE486_04745"
     CDS             complement(2487..2804)
                     /locus_tag="VE486_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZA38427.1"
                     /translation="MPPRRGEVWLFDCGIVAKVRPVLILSIPFADTDRAVITVVFHTT
                     TLRGSDFEVRIKIPFLKEGAFVAQSIATYPIARAIRKLGVLNETQLAEVEAAVFKWLG
                     RSA"
     gene            complement(2794..2982)
                     /locus_tag="VE486_04750"
     CDS             complement(2794..2982)
                     /locus_tag="VE486_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38428.1"
                     /translation="MSTATARLLSEFEALPVEEKQEFVREVINHLPPWDSGPLNDDAV
                     AAAGDALAEKLDEGERAS"
BASE COUNT          742 a          893 c          904 g          651 t
ORIGIN      
        1 tgctcacacg atcgcagtca tcctttcgtc aatcacatct cttttcgcgc atgggacagc
       61 ttcgaaagcg gaacccgcga aaacgaagtt cagcaaaaaa tctcagaagt cagcagacga
      121 ccgtctgctc aatgtcggtt caattgcgtt ctctccggga cagctatcgg caacttcgcc
      181 caagccagga gagcgatatc cgtggaaaaa gagtattgtg acaacggttt tttggatcgg
      241 cgaaaaaccg agcgaaaata atccggtgcc gaatcggacg agctcgtggg acaaaaattg
      301 gacgaaaaat tacggtggcg tcgatgatcc gaacccggca aacagaagca attatattcc
      361 ggcgaaattc acgccacgcc aaaatccatt ttattgcgca cttccctaca atgataaggc
      421 aagagagggc catcgccccg aagcgccgcg cgttgttccc tggtttaata atgcatacca
      481 ggggccggcg gtctcgactt gcaaaggtcg atgggtcgca atccgcaaag gcaatcgaac
      541 cgtttacgcg caatgggaag acgccggacc gttccgcacg gatcactggc aatatgtgtt
      601 tggcaatgag cggccaaaac ccaatctgaa taagggagct ggtctcgatg tctcgccggc
      661 ggtccgcgac tatctcggaa tgaacgacac agatgtgacg gactggaggt tcgtggagtt
      721 tagtgaagtc ccgcgcgggc catggtcgac tcatggagaa aacaacacgt tcgtggtcaa
      781 cgatcgcaaa aaaggggtca agctggccga ggcgtccaag cccggcggcg gcccaattgt
      841 gcgttaacgt gcggttccga aacgcgctgt agccgggatc gctgatcccg gccacgaatg
      901 cccgggtagc ggtcgccgcg gcgacttggt ttcggcatcg cgccgaaaca gtctttgctt
      961 tgggtgggaa ttgtaggacc gatcaagcgc acaacaaaag ttcgcaatcg cgggacgcgc
     1021 tcgccaggtc gccgcggcga ccgcacccga tccacgcaat ctgcgagtcg gaagcgatta
     1081 caaagaacaa atgggaaatc gctgcgcagt tcgtcttctt tattgcgcga tctttatcgc
     1141 atgcaacctc agggttgctg ctcagaccga aacggcttcc gagaacgcca accttcttac
     1201 cggcaaagcc gcgatgggcg attggaccgc cgatgcgcct ggagtgcgtc gtagaatcac
     1261 cgtcaaggac ttgcctgcgc ccagttcaaa tgttttcgca attaaccggc cgggggtggt
     1321 cgaacgtcca gaaggggcgc agcctcaggt cccgcccggt tttaagatcg aaatgtacgc
     1381 caacgggttt cgtgaccccc gatatctgcg taccgcgccg aatggagata tcttcgtcgc
     1441 ggatagtcgg ggtgccaatc gaattagggt gctgcgcgat acaaagggca gcggtaagcc
     1501 tgacgtcacg gaaacattta ccgaagacga tctaaacaaa ccatttggga tggcatttta
     1561 tccgcccggc aacgatcccc aatttttata cgtcgcgaat accgacggtg taattcgatt
     1621 tccgtaccgg aatggcgacc tcaaagcccg cggaccagcc gaaaagttga gtgcgcatct
     1681 ttccggtggc gcaggaaacc tgcggagcgg cggacattgg acgcgcgaca tcgttttttc
     1741 gccggacggc aaaaaaatgt acgtctcgat cggatcacgc tcgaacgtct ccgataatgc
     1801 tgcggaagct gatcgcgctc gtatttttga gttcaacccc gatggtagcg ggcgaaaagt
     1861 ttatgcctgg ggcgttcgga acgcggtcgg gatcgcgttt cggcccggca gcgacgagct
     1921 ctggatgagc acgaacgagc gtgacgaaat cggtgaggat ctaccgcctg attacatcag
     1981 cagtgtgcgg cccggtggtt tttatggctg gccttggttt tacattggca accacgtcga
     2041 tccgcgccac aaagggaaac atcctgaact cgccgacaaa gtgatcgtcc ccgacgttct
     2101 ggtggaggcg cactcggcca cgcttagttt gtgcttttac accggcgatc aattcccagc
     2161 cgaatacaaa ggcgacattt tcgccgcatt ccacggttca tggaaccgaa agaaacgcac
     2221 cggctacaaa attgtccggg tgccctttga cgacgggaaa gcactcggtg aatacgagga
     2281 tttcgtgacc ggatttgtca cccccgacgg acaagtgtgg ggtcgcccgg tcggaatcac
     2341 cgtcgctaaa gacggcagcc tccttttcag cgaagacggc aacggcacca tttggcgcgt
     2401 gagctacggc aagtagctct ttgatacacg cagcctgcta aaagcttccc ttcaatcgtt
     2461 ccgcgagctc ttgcttaaaa gccatctcat gcagaccggc cgagccactt gaacacggca
     2521 gcttcaactt ctgcgagctg cgtttcgttc aacacgccga gtttgcgaat cgcgcgagcg
     2581 atcggatagg tggcgatgct ttgcgcaacg aacgcgccct ctttaagaaa ggggatcttt
     2641 attctgactt caaagtccga accgcgcagt gttgtcgtgt ggaagacaac ggtaatgaca
     2701 gcgcgatcgg tatcggcaaa gggaatgctg aggatcagaa ctggcctgac tttcgcgacg
     2761 atgccgcagt cgaacagcca gacttcgccg cgcctaggag gcacgttccc cctcatcgag
     2821 cttctcagcc agcgcgtcac ccgcggcagc aaccgcgtca tcgttcagcg gcccggaatc
     2881 ccacggcggc aggtggttaa tcacctcccg tacaaactcc tgtttttcct cgactggtaa
     2941 cgcctcgaac tcggaaagca gtctcgctgt ggccgtactc atgcagaaaa tttagcgggc
     3001 cttggtgagg ttgtcagccg cgaagttgga ggccattgct accattttgc ccaggcgttt
     3061 cgctcggttc ccccaaaaac gatccgcatt tcatatgaca atccggcagg tgtagcggcg
     3121 gcggagcctg ccccgagcta gtcgaacgcg tcaaccgcaa aatctgcagc ggcaggcgtg
     3181 cctgccatgc
//
LOCUS       DASQUQ010000166         4994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_398751, whole genome shotgun sequence.
ACCESSION   DASQUQ010000166 DASQUQ010000000
VERSION     DASQUQ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398751"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VE486_04755"
     CDS             complement(<1..677)
                     /locus_tag="VE486_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38429.1"
                     /translation="MNYLVSIGLEVHVQLKTRSKMFCACPVEFGAEPNTHTCPVCLGL
                     PGALPVMNHEALRMTVLTGLMLGCDIAPICKFDRKNYFYPDMSKNYQISQYDMPLCTN
                     GIVPLHLLAYPKDAQKDIATPEKEVHLVRIHLEEDVAKSFHFAKSTGIDFNRAGTPLM
                     EIVTQPEINSPEEAFAFLNSLKQILIYGAVSDADMEKGQLRCDCNVSVRPEKQAELGA
                     KIEIKNM"
     gene            complement(892..1818)
                     /locus_tag="VE486_04760"
     CDS             complement(892..1818)
                     /locus_tag="VE486_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZA38430.1"
                     /translation="MEVEPELQAVAGSPLWQAIFISFAVVLILFEVVRGWRLGLIRQL
                     VRVAALGAAYAAAIFGGRLLLPIARPFLKMPDIVLSILGGAVLAFVIYALVTSMGMML
                     FKQTGQQNSRLVQLIYGIAGAIVGLFFGAFVLWLIVAGVRAIGALADAQVRSRANPPE
                     AGQSATTRALEVRRRFLSEPNAGSTSLATSLARLKNSLELGPLGNAFKQTDPVPLKVY
                     ETLGKVGSVFSSPERAQKFLTFPGARELSEHPRIVALRDDPEISEMIAQGRFLDLLQN
                     QRIIDAANDPTLADQIKKFDLQRALDYAAEQK"
     gene            complement(1818..2906)
                     /gene="aroC"
                     /locus_tag="VE486_04765"
     CDS             complement(1818..2906)
                     /gene="aroC"
                     /locus_tag="VE486_04765"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZA38431.1"
                     /translation="MGNTFGQLFRVTTFGESHGGGIGVIIDGCPPKIDISEAEIQREL
                     DRRRPGQSKLTTQRKEEDRCEILSGVFKGETLGTPIAILVRNKDARPEDYVEIATKFR
                     PSHADFTYEAKYGIRNWQGGGRASARETIGRVAAGAVAKKILSILCSKFEIVAYVAKI
                     HEIIANINRSVVKMKDVEKNAVRCPDAAAAKKMASFIEQVRGEGDSVGGVIECVARGI
                     PPGLGEPVFDKLEADLAKAMLSLPATKGFEIGSGFRATRMRGSEHNDPFEMRGGKIRT
                     ATNNSGGVQGGISNGEEIYFRVGFKPTATIAYEQKTVTSSHEQTRLAARGRHDPCVLP
                     RAVPIVEAMAAIVLCDHALRQKAIGLLG"
     gene            complement(3020..4180)
                     /locus_tag="VE486_04770"
     CDS             complement(3020..4180)
                     /locus_tag="VE486_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZA38432.1"
                     /translation="MKKQHEFATRAIHAGQEPDPSTGAIMTPIYATSTYVQESPGKHK
                     GYDYARSINPTRLAYERCVADLENGTRGFAFASGLAAMSTALEVLESGSHIVASDDLY
                     GGTFRLFEKVRRRSANLDFAYVDLTDPEDFERAIKKNTRMVWIETPSNPLLKLIDLEA
                     IAKIARDHKIVSVCDNTFATPWIQRPIETGFDLVIHSATKYLNGHSDMVGGVVVVGEN
                     KELGDQIAFLQNSVGAIAGPFESFLVMRSLKTLALRMERHCSNAIEIARWLEEQPQIE
                     SVSYPGLKSHPQHDLARQQMRGFGGMVTIILKTDLGGTKRFLENTHLFSLAESLGGVE
                     SLINHPALMTHASVPREQREALGVTDSLVRLSVGVEDVRDLIDDLESALDMI"
     gene            complement(4245..>4994)
                     /locus_tag="VE486_04775"
     CDS             complement(4245..>4994)
                     /locus_tag="VE486_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZA38433.1"
                     /translation="ILYEYVKTGKLAEAGSWAVEGIGEDFVPDIADMSLVTDAFEIDD
                     EESFATARELLRKEGIFGGSSTGTLVAGALRYCREQTKPKRVVSFVVDTGNKYLSKMY
                     DDFWMAEQGFIHRPPHGDLSDLISHRYEAGEVISVGPTDTLLTAFKRIRDKDVSQLPV
                     VDQNGRAIGILDESDVLVKVHRDPSHFNDPVKNAMTDKLETLPPTAKINDLLKVFDRG
                     RVAIVMDNNKFLGLITRTDLLSYLRLHMPKQ"
BASE COUNT         1125 a         1428 c         1312 g         1129 t
ORIGIN      
        1 ttcatgtttt tgatctcgat cttggctccg agttcggctt gtttttcagg tcggacggaa
       61 acattgcagt cgcatcggag ctgccctttc tccatatcgg catcgctcac ggctccatag
      121 atcaggattt gtttgagcga atttaagaaa gcgaacgctt cctcgggcga attaatctcg
      181 ggctgggtca caatctccat taaaggcgtg cccgctcggt tgaaatcgat tcccgtgctc
      241 tttgcgaaat ggaaactctt cgccacatcc tcctcgagat gaatgcgcac gaggtgcacc
      301 tccttctctg gtgtcgcgat atctttttgt gcatccttcg gataagcgag gaggtgcaac
      361 ggcacgatgc cattggtgca aagcggcatg tcgtattgcg aaatctgata attcttcgac
      421 atgtctggat agaaataatt cttccggtcg aacttacaga tcggcgcgat gtcgcacccg
      481 agcatgaggc cggtgagcac ggtcatacgc agggcctcat gattcatcac cggcaaggcg
      541 cccggcagcc cgagacagac cgggcaggtg tgcgtgttcg gttcggcgcc aaattcaacc
      601 ggacacgcgc aaaacatttt cgagcgcgtc ttgagttgaa cgtgcacttc gagaccgatg
      661 ctaacgagat agttcactgg ccctcagaag ttaaccacga atgaacacga atcgacacga
      721 atgaagcacg ggacaatgac gaagcacgaa taacgaatgc cgaaggaata aaaaaatagc
      781 aaatgacgaa tccaaagcgg gacggtttag ccctgtcatt tgggtttcgt cattgattca
      841 tcattcgtca ttcggatttc gtcattctac ggaattcgtg tttagtggtg gttacttttg
      901 ctcggcggcg taatcgagtg cgcgctgaag atcgaatttt ttaatctgat ccgcgagtgt
      961 cgggtcattt gccgcgtcga tgatgcgttg attctggaga agatcgagaa agcgcccctg
     1021 cgcgatcatt tcagaaatct ccgggtcgtc ccgcaaagcc acgatccttg gatgctcgct
     1081 taactcccgc gcacccggga acgtgaggaa tttctgcgcg cgttccggac ttgaaaatac
     1141 gctgccgact ttcccgagcg tttcatacac cttcaacgga acggggtccg tctgcttgaa
     1201 cgcgtttccg agcggcccca gctcgagcga attctttaag cgggccaatg acgtagcaag
     1261 cgaagtagat cccgcatttg gctccgagag aaaacgtcgc cgcacctcga gggcacgtgt
     1321 ggtcgcggac tgaccagcct ccggaggatt tgcccggctg cggacttgcg cgtcggccag
     1381 ggcgccaatg gcacgaacgc ccgcaacgat tagccagagg acaaaggcgc cgaaaaacaa
     1441 tccaacgatt gcccctgcga ttccataaat aagctggacg agcctggagt tttgttggcc
     1501 ggtctgtttg aataacatca ttcccatgct ggtcacgagc gcatagatga cgaacgcaag
     1561 cactgcgccg cccagaatcg acagaacaat atcaggcatt ttcaaaaacg gccgcgctat
     1621 cgggagaagc aatctcccgc cgaaaatcgc ggccgcatac gccgcgccca gcgccgcgac
     1681 acgcacaagt tgacggatca aaccgagtcg ccagccccgc acgacttcga acaatatcaa
     1741 aacgactgcg aaagagatga agattgcttg ccaaagcggc gaaccagcca ccgcttgaag
     1801 ttcgggttcc acttccatca acccagcaaa ccaatcgctt tttgccggag cgcgtgatcg
     1861 caaagcacga tcgcggccat cgcttccacg atgggaacag ctcgaggaag aacacacgga
     1921 tcatgacgcc cgcgtgccgc gagcctcgtc tgctcgtgcg aggaggtcac ggttttttgt
     1981 tcgtaggcga tcgtggctgt cggcttaaag ccaactcgaa aatagatctc ctcgccgttg
     2041 ctgattccac cttgcacgcc ccccgagttg tttgtcgctg tccggatttt cccgccgcgc
     2101 atttcaaatg gatcgttatg ctccgagccg cgcatgcgcg ttgccctaaa cccggaaccg
     2161 atctcaaatc ctttggtcgc cggcaggctc agcatcgctt ttgccaggtc tgcctcgagc
     2221 ttgtcaaaaa ccggctcgcc caagcccggt gggatcccac gagcaacgca ttcgatcacg
     2281 ccacctaccg agtcgccttc gccgcgaact tgctcgataa acgacgccat cttcttcgcg
     2341 gccgcggcat ccggacagcg caccgcattt ttctccacat ccttcatttt cacaacggaa
     2401 cgattgatgt tcgcgatgat ctcatgaatc ttcgcaacgt aagcgacgat ttcgaacttc
     2461 gagcacaaga tcgacaaaat cttcttcgcg actgcgcccg cagcgacccg gccaatggtc
     2521 tcgcgcgccg acgcccgacc tcctccctgc caattgcgaa ttccatattt ggcctcgtaa
     2581 gtaaaatcag catgtgaggg tcgaaatttt gttgcgatct ccacgtaatc ctcgggccga
     2641 gcgtccttgt tgcgcaccag aatggcaatg ggtgtgccga gcgtttcgcc tttgaatacg
     2701 ccggaaagga tctcgcaacg gtcttcctct ttccgttgcg tggtaagttt gctttgcccc
     2761 ggccgacgcc gatccagttc tcgctgaatt tccgcttcgg atatgtcgat cttcggaggg
     2821 cagccatcaa tgattacgcc gatgccgccg ccatgcgatt caccaaacgt ggtaacgcga
     2881 aaaagctggc cgaacgtgtt gcccattgag atggaagata gccatcctgg ctgtctcggc
     2941 gagcaggctt ccagcctgcg tctctacggc aggcaggatg cctgccggcc aagacaggca
     3001 agattcctgt cctccaaaac taaatcatgt cgagcgcgct ttcgagatcg tcgattaaat
     3061 cgcgcacgtc ttcgacgcca accgacaagc ggaccagcga atcagtcacg ccgagcgcct
     3121 cacgttgttc cctgggcacc gaggcatgcg tcataagggc ggggtgattg atcagactct
     3181 cgacaccgcc caggctttcc gccagcgaaa atagatgcgt gttctccaga aaacgcttcg
     3241 tcccaccgag atcggtcttc aaaataatcg tcaccatgcc gccgaagccg cgcatttgct
     3301 gtcgggcgag gtcgtgttgc ggatgcgatt tcaggccggg gtaactgacc gattctattt
     3361 gcggttgttc ctccagccag cgcgcaattt cgatcgcgtt ggaacagtgc cgctccatgc
     3421 gcagggcaag ggttttcaaa ctgcgcatga ccagaaagct ttcgaacggc ccggcaatcg
     3481 cgccaactga attttgtaaa aacgcaatct ggtcgccgag ctctttgttc tcaccgacta
     3541 caacgacgcc accgaccatg tcggaatgtc cgttcagata tttagtggcg gaatggatca
     3601 ccaaatcaaa gccggtctcg atcggccgct gaatccatgg agtcgcaaat gtgttatcac
     3661 aaaccgaaac gattttgtga tcgcgcgcga ttttcgcgat cgcttctaga tcgatcagtt
     3721 tgagcagcgg attgctcggc gtctcgatcc acaccatgcg ggtgttcttc ttgatggcgc
     3781 gctcgaaatc ttcaggatcg gtgagatcga cgtaggcaaa atcaagattg gccgagcggc
     3841 gtctaacttt ctcgaataag cgaaacgtgc ccccgtaaag atcgtcactc gcgacgatat
     3901 gcgagccact ttcgagcact tccagcgcag tggacatcgc ggccagtccg gatgcgaacg
     3961 caaatccgcg ggtgccgttt tccaaatcgg cgacgcaccg ttcataagcc aaccgcgtcg
     4021 ggttaatgga gcgcgcgtaa tcgtagcctt tgtgcttgcc cggactttcc tgaacgtatg
     4081 tggatgttgc gtaaatcggc gtcatgatcg ctcccgtgga cgggtcgggc tcctggccgg
     4141 cgtgaatcgc tcgggtggca aactcgtgct gctttttcat ctcgatctaa tctaactgcg
     4201 gattacagca aacatgacag cgaatcatcc gcgcaattag cgaatcactg tttcggcatg
     4261 tgcagacgta aataagaaag caggtcggtt cgggtaatca gcccaagaaa cttattgtta
     4321 tccatcacaa tggctacacg accgcgatcg aaaaccttca gcagatcgtt gatttttgcc
     4381 gttggcggga gcgtttcgag cttatcggtc atcgcatttt tcaccggatc gttaaagtgc
     4441 gaaggatccc gatgcacttt cacgagcaca tcggattcat caagaatccc gatagcgcgt
     4501 ccgttttgat cgacaaccgg gagctgcgaa acatctttgt cgcgtatccg cttgaatgcg
     4561 gtcagcagcg tatcggtcgg cccgaccgaa attacctcgc cggcttcgta gcgatgggaa
     4621 atcaaatcgc tcaaatcgcc atgtggcgga cgatgaatga atccctgctc cgccatccag
     4681 aaatcgtcgt acatcttgga aagatatttg tttcccgtat cgaccacgaa gctgacgacg
     4741 cgtttcggct tcgtttgttc gcggcaataa cgcagcgcgc cggccacgag cgtgccagtg
     4801 gatgagccgc cgaaaattcc ttctttgcgt aacagctcac gcgcggttgc aaaactttcc
     4861 tcgtcatcga tctcgaatgc gtcggtcacg agtgacatgt ccgcgatgtc gggaacgaag
     4921 tcctcgccga tgccttcgac cgcccagctt cccgcctccg ccagtttgcc agttttgaca
     4981 tactcgtaaa gaat
//
LOCUS       DASQUQ010000167         4577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_128814, whole genome shotgun sequence.
ACCESSION   DASQUQ010000167 DASQUQ010000000
VERSION     DASQUQ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128814"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VE486_04780"
     CDS             <1..289
                     /locus_tag="VE486_04780"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZA38434.1"
                     /translation="ELREEAGYQLAKDGKLIALGDYFTSPGFTDERGYFFLARPVEPI
                     ANYVRDETESILDCRAFSVPEIRRMIAENEIRDANTLGMWARLLACDLLPG"
     gene            386..1333
                     /gene="tatC"
                     /locus_tag="VE486_04785"
     CDS             386..1333
                     /gene="tatC"
                     /locus_tag="VE486_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZA38435.1"
                     /translation="MPRTFSGSRQPDQFRLAKIKRTFPIPPSDLFVFIRVIRGLSYRS
                     AEYLAKPIIKPMALRDIFKFRELPESETTKPFLEHLEDLRWTVVKMAITLVIAMIICF
                     AFRSLLVRVMQAPLQEVGSQIGTLKALGVTDSLVISFHLAFYAGIVLSFPLLLYFLAE
                     FVLPALTAMEKRFVLPAIFASFGLFLLGVIFCYYWLLPKTILFFFRDTESLGWAPTWT
                     VQQYYSFVTRFTIGFGLAFELPVVVLALVRFGLLTYQFMARTRPYAIVLIFILATIIT
                     PTPDVMTLIAMGLPMYLLYESCIWIAWFIERRRARSAAG"
     gene            1408..2550
                     /gene="mtnA"
                     /locus_tag="VE486_04790"
     CDS             1408..2550
                     /gene="mtnA"
                     /locus_tag="VE486_04790"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZA38436.1"
                     /translation="MQRSSSKSMKVGGQHFRTIWLDTEDQCVVQLIDQRFLPHRFVIE
                     KVGTVEQMVRAIRDMHVRGAGLIGASAGYAMYLATIEAAGGPAVPGFDQQLANAAAEL
                     KATRPTAVNLSWAVERQLKNIAEGKTAEEKITLALRTAKQIAEEDVEHCRMIGEHGLN
                     LIRQIVRKKAGKPVSVLTHCNAGWLAFVDYGSATAPIYAAHDSGLPVHVWVAETRPRN
                     QGSKLTAWELGEHGVPHTVIADSAGGHLMQHGDVDLVIVGTDRTTYTGDVANKIGTYL
                     KALAAKDNDIPFYVALPSSSLDWKMRNGLEEIPIEERGAEEVKHADGWLEGRQVEVRV
                     TPERSPAANYGFDVTPRHLVTGLITERGVCEANEQSIRALFPEHTP"
     gene            2547..3182
                     /locus_tag="VE486_04795"
     CDS             2547..3182
                     /locus_tag="VE486_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZA38437.1"
                     /translation="MSKYIKFACEGAATEITPFDGFAELNACRRRLLQLRLIGVDANG
                     VGFGNLSVRDGATKNFYITGSATGGKPELTLADCARVVAYDFKRNCLRYEGTAIPSSE
                     SLTHAAVYEADTKAGSVMHCHDSKLWAALLNEAPTTSEAVDYGTPEMAYEVTRLFKVT
                     DAQSRKILVMAGHEGGIVAFGKDLEEAFVVLMRERKEREEIELNGKDVRDY"
     gene            3301..3927
                     /locus_tag="VE486_04800"
     CDS             3301..3927
                     /locus_tag="VE486_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZA38438.1"
                     /translation="MRKKTQEDPPRSRLLVLVDESNVGSSVRTAGRGLDWLKLRDFLA
                     GPATGRELIEMVVYAGLPPAIPIWQEERDKKNKFMHWLRSNGFMVVTKDGSPTEEGRY
                     KANVDVMMAIDALELSVEMRPDVVILVTGDADFAYLAIKLRRRGIRVEVASVAQNLGN
                     ILKSAANEVIDLAPLFRMFEMMKIPDSAPQQQQQQRRRTSTNRPSQFR"
     gene            complement(4101..4430)
                     /locus_tag="VE486_04805"
     CDS             complement(4101..4430)
                     /locus_tag="VE486_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZA38439.1"
                     /translation="MQRKRYLFVCINRRPADNPKGCCAAKGSEEVYKALKAEVAARGL
                     ARLEARVCTSSCLDQCATGITILVEPDHFFYGRVTIADVSEIVDGLVSGHRVERLVLS
                     AEDLEKV"
BASE COUNT         1013 a         1185 c         1350 g         1029 t
ORIGIN      
        1 ggaactgcgc gaagaagccg gttaccagct ggccaaagat ggcaaactca tcgcgctcgg
       61 cgattacttt acctcccccg gattcaccga cgaacgcggc tattttttcc tggcgcgtcc
      121 ggttgaaccg atcgcgaatt atgtgcgcga tgaaacggaa tcgattctgg actgccgcgc
      181 gttcagcgtt ccggaaattc gccgcatgat tgcggagaac gagattcgcg acgccaatac
      241 cctggggatg tgggcgcggc tcttggcgtg cgaccttttg cctggttaag gaacactaga
      301 ctagcggtct tctaacagcc cgcgaatcac gcgaattgac gcgaaattag ttgaaggaag
      361 gcatttattc tgaagctcgg cgcatgtgcc ccgaaccttt tcgggaagcc ggcagccgga
      421 tcagtttcgg ctcgcaaaga tcaaacggac ttttccgatt cccccttctg atctattcgt
      481 gttcattcgc gtgattcgcg ggctgtccta ccggtcggcc gaatacctcg ctaaaccaat
      541 catcaagcca atggcgttaa gagacatttt caaatttcgt gagttgcctg aaagcgagac
      601 aaccaagccg tttctggagc atctcgaaga cttgcgatgg accgtcgtga agatggcaat
      661 cacgctcgtt atcgccatga tcatttgttt cgcgttccgc tcgctcctgg tgcgggtcat
      721 gcaagcgcca ttgcaagagg tcggttcgca gatcggcacg ctcaaggcgc ttggcgtcac
      781 ggattcgctc gtgatctcgt ttcacctcgc gttttacgcg ggaatcgttc tttccttccc
      841 gctgcttctt tattttctcg cggaattcgt tctgcccgcg ctcaccgcaa tggagaagcg
      901 cttcgtgctt ccagcgattt tcgcgagctt cgggcttttt cttcttggag ttattttctg
      961 ttattattgg ttattgccga aaacgattct gtttttcttt cgcgacaccg agtcgctggg
     1021 ctgggcgccc acctggactg tgcagcagta ttattctttt gtgacgcgat tcacgatcgg
     1081 tttcgggctc gcgtttgaat tgccagtggt ggtcctggca ctggtgcgat tcggattgct
     1141 tacttaccaa tttatggcgc gcaccaggcc ctacgccatc gtgcttattt ttatcctggc
     1201 gacgatcatt acgccgacgc cggacgtcat gaccctgatc gcgatgggtc ttcccatgta
     1261 tctgctttac gaaagctgca tctggattgc gtggttcatc gagcgccgcc gcgccagatc
     1321 ggctgccggc tgaccgaata ccctacctct ttgccgagca gactggccat ggttgcgcgt
     1381 ttgagcgatt agtgagctaa ccagtaggtg cagagatcat catcaaaaag catgaaagtg
     1441 ggcggccaac attttcgaac gatctggctt gatacagaag atcaatgcgt cgtgcagctg
     1501 atcgatcagc gatttttgcc gcaccgtttc gtgattgaaa aagtcggcac ggtcgagcag
     1561 atggtgaggg cgatccgcga catgcatgtt cgcggagcgg ggttgatcgg cgcgagtgcg
     1621 ggatacgcga tgtatcttgc gacaattgag gccgccgggg gtcccgcagt cccgggattc
     1681 gatcagcagc tggccaatgc ggcggcagaa ctcaaggcaa ccagaccgac agcggtgaat
     1741 ctttcctggg cggtcgagcg ccagctaaag aacatcgccg agggaaaaac tgcggaagag
     1801 aagatcaccc tggctctgcg cacagccaaa caaattgccg aagaagatgt ggagcactgc
     1861 cggatgatag gagagcacgg gttgaatctc atccgtcaaa ttgtccgaaa gaaggctggg
     1921 aagccggtca gtgtgcttac gcattgcaac gccggctggc tcgcgttcgt tgattacggt
     1981 tccgcgacgg cgccgatcta tgccgcgcac gatagcgggt tgccggttca cgtgtgggtc
     2041 gctgagacca gaccaaggaa tcaaggttcg aaactgactg cctgggaact gggtgagcac
     2101 ggcgtcccgc acacggtaat cgcagatagt gcgggcgggc atttgatgca acatggcgat
     2161 gtggatctag tgattgtcgg aaccgatcgc acgacctaca ccggagatgt tgcgaacaag
     2221 atcgggacgt atctgaaggc gctggcggcg aaggataatg atattccttt ctacgtggcg
     2281 ctgccttcgt cttcgcttga ctggaagatg cggaacgggt tggaagaaat cccgatcgaa
     2341 gagcgcggag cggaggaagt aaagcacgct gacggatggc ttgaggggcg acaggtggaa
     2401 gtgcgcgtta cgcctgagcg aagtccagct gcaaactacg ggttcgatgt tacgccgaga
     2461 cacctggtga ccggactgat taccgagcga ggcgtgtgcg aagcgaacga gcaaagcatc
     2521 cgcgcgcttt ttcccgagca cacgccgtga gcaaatacat aaagttcgcg tgcgaaggcg
     2581 cggctacgga gatcactcca ttcgacgggt ttgccgaatt aaacgcgtgt cggcgaagac
     2641 ttcttcagtt gcgactgatt ggtgtggatg caaatggcgt tgggttcggt aacctgagtg
     2701 taagggacgg cgcgacgaag aatttttata tcacaggttc ggcgacaggc ggaaagcctg
     2761 aactgacgct ggcggattgc gcgagagtgg tggcttacga tttcaaaagg aactgcctcc
     2821 gatacgaggg gaccgcgatt ccttcatcgg aatcgctgac acacgccgcg gtttacgagg
     2881 cggatacaaa ggccgggtcg gttatgcatt gccacgattc aaagttgtgg gcagctcttc
     2941 taaatgaagc tccgacgacg tcggaggccg ttgattacgg gacgcccgag atggcgtacg
     3001 aagtgacgcg tcttttcaag gttactgacg cgcaaagcag aaagattctt gtcatggccg
     3061 gacacgaggg aggcattgta gctttcggaa aggatctcga agaggcgttc gttgttctaa
     3121 tgcgcgagcg gaaggaaagg gaagagattg aattgaacgg aaaagacgtc agagactatt
     3181 gactcttctg ccgtcaattc gtccagcttc ggtaaaacaa ttgtttcgca attcacggcg
     3241 aaaccgttga agaattttgt gttcgctaga agtgcgcgga cctagttcac tcggacctgc
     3301 atgcgaaaga aaactcagga agatcccccg cggtccagac tattggttct tgtcgatgaa
     3361 tcgaatgttg gcagctccgt gcgcaccgcg gggcgcgggc tggattggct gaaactgcgc
     3421 gactttcttg ccgggccagc cacaggacgc gagttgatcg aaatggtggt gtatgccgga
     3481 ctcccgcccg ccatcccgat ctggcaggaa gaacgcgata agaagaacaa gttcatgcac
     3541 tggctgcgct ccaatggatt catggtcgtc acgaaagacg gatcgcccac cgaagaaggc
     3601 cgttacaagg cgaacgttga cgtgatgatg gcaatcgatg cgctcgagct ttcagtcgag
     3661 atgcggccgg atgtcgtcat tctcgtaacc ggcgacgccg attttgctta tctcgccata
     3721 aagttgaggc gccgcggcat ccgcgtggaa gtggcctcgg tcgcgcaaaa tctcgggaac
     3781 atattgaagt cagccgcaaa cgaagtaatc gatctggcgc cattgttcag gatgttcgag
     3841 atgatgaaga tcccggattc tgctccgcaa caacagcaac agcagaggcg aagaactagc
     3901 actaatcgtc ccagccagtt ccgctaaaga ctgcccaacg tctcataaat cgctgcctga
     3961 tttgtttgtt cacggtatgt tgcgccgccg cggttcgatg taggagcaga atcgaacaac
     4021 cgaaccgcag ccagcagcag cggctacggc tgcagttgct taaacgtctc agccgacacg
     4081 accgccgccg cgatagcccc tcaaactttt tccaaatctt cggcgctaag caccagccgt
     4141 tcgacgcggt ggccgctgac gagtccgtct acaatctcgg acacgtcggc gatggttacg
     4201 cgcccgtaaa aaaaatgatc tggttcgact aaaatggtga tgccggtcgc acattgatcg
     4261 aggcagctcg acgtgcacac gcgcgcttcg agtctggcga gtccgcgcgc ggccacctcc
     4321 gctttcaatg ctttgtacac ttcctccgac cctttcgccg cgcagcatcc ttttgggttg
     4381 tcggcggggc ggcgattgat gcaaacaaag agataacgtt tccgttgcat aggggaaagc
     4441 tgtagaggca gccgtgccgc ctgcagttgt ctaatgttgc gcccggaaaa agtgatagct
     4501 agggactgtg ttggtcagcg ccgaattcgt gatgattctg cttttgaatg gtccggcaaa
     4561 ggcaaaccct gtggcaa
//
LOCUS       DASQUQ010000168        10222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_687167, whole genome shotgun sequence.
ACCESSION   DASQUQ010000168 DASQUQ010000000
VERSION     DASQUQ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687167"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1581
                     /locus_tag="VE486_04810"
     CDS             181..1581
                     /locus_tag="VE486_04810"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZA38440.1"
                     /translation="MKTENLRILIVDDERPILMTLEALLKRHGYQVDTAPTASQGLKL
                     LKSKSPSLVLLDLQLPDAEGLEMLDQIKTERPEMQVIILTAHDSLNNAIESIKRGAYH
                     FISKPYAPEELLSLVEKALEKQFLVREAEQLRQRTEQLEKQLEIAETRATPIFNSKRM
                     QEIEELINAMAPSDANVLIVGESGVGKEVIANAIHTRSRRAGKEMVKLNCAAFPQTMI
                     EGELFGYVKGAFTGAMNDFPGMIAAAGGGTLFLDEISDMPPDLQTRFLRVLQEREYRP
                     LGSTRTMKADIRVIASTNRPVAQALLEHRLRSDLYYRLNTFQIEVPALRERKQDIPPL
                     IGLFLKQFAQQLGKPEPDIAPDAFQKLLDYAWPGNVRELQNAIEYAVVLARQGLIGVK
                     ELPAEVQLPTVLQLTERAPAAAGRSGVQSLDDVERNTILQALAECHGNKKKAAERLGI
                     QRATLYNKIKRYGIEI"
     gene            2078..4882
                     /gene="glnD"
                     /locus_tag="VE486_04815"
     CDS             2078..4882
                     /gene="glnD"
                     /locus_tag="VE486_04815"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZA38441.1"
                     /translation="MATSRHLEKVLAHAANQLAATGARRPTEVLPLYKKFLNVEEHRL
                     RLKHQAGGGGREICARRADLVDVLLQYLFGAAATVTRGNGESRIGLALIALGGYGRGE
                     LNPFSDIDVMLLHDQGSKKISPHLEEMVQQVLYPLWDSGFKVGHSTRSIKDAIAQANR
                     DMRTKTAMLESRFLAGDEELAREFREQFRSKCVEGYERDYVEMRMQDQVARHKKFGDS
                     VYLQEPHVKSGCGGLRDYQNLLWMTYFKEGSLSTNQLVGKDWLSESDQRQIERAYEFL
                     LRLRTDLHYATGRATDILHMNLQEQIAERLNYSARNGQLRSETLMRDYYEHTRNIFRV
                     TERITEQFVSGHVTSRTRSLFSFLPLIRPEKVPIGDSFFVRNNQLYPARRDVFRKDPE
                     QMMRAFQFAQERGLDLSPELADLLSRSLGQVTRTYQYARGPREIFKTILSRKGEVGRI
                     LRMMHRVDFLGRYVPEFGQLTCLVQHEFMHRYTADEHTLVCIDKLDALATTDDQKLIA
                     YRRIFEQLDDPFVLYLALLLHDSGKAVGARRHSEASALFAQRVAARLQLSSEQRKSLI
                     LLVDHHLTLSMIAQQRNLDDPATVAEMAGNVKNQKNLNALMLLTLADGQGTSAEAWSD
                     WKESLVWQLFHETSRYLADRQSYYEQTRIERESLRRAVAQDLSPDYADEIDAHFDFMP
                     DNYFRGRDVPEIVEHLKLFRSFVEQVASSPQDGFAAANRGGHPLAPAVKWKALPEQGH
                     SVVSFCTWDRERLLAKIAGSFSVVPINILSADVFPRGDNVVLSVFRVCDTKAGAVTDP
                     RAFELVERTLRSALDDEHFDFLPLIEKAKRQIPHRAPGIEFPTRISMDNKTHPAYTLI
                     EIQAPDRLGLLYDILTCLDRESVSIPLSRINTQDGAAIDTLYVVDRSTDARITDPDRI
                     RTIHEHLTSTILGGATAKAT"
     gene            4932..5678
                     /locus_tag="VE486_04820"
     CDS             4932..5678
                     /locus_tag="VE486_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZA38442.1"
                     /translation="MNALILAAGYATRLYPLTLDKPKPLLAVGGKPIIEWTMDNLADV
                     PDLETIYIVTNDKFASDFQAWSERYRSRHPKFKLKIVNDGSQSDDDKLGAIGDINFVV
                     SRENLTSDGLIVIAGDNLFTESLTGFVACAKETEATVAVYDVGDTEAIKEYGNIAVDA
                     DGIITHFEEKPQKPRGTLAAIALYYYSPEVLSLLTTYLAAGNNPDQPGRFVQWLYTRR
                     PVKTFQIKGQWLDIGSKEMLKKADEILSRA"
     gene            complement(6113..6892)
                     /locus_tag="VE486_04825"
     CDS             complement(6113..6892)
                     /locus_tag="VE486_04825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZA38443.1"
                     /translation="MRFKGIARPIAAPGNETPLNMATVSEQAGDLLSDFCGAITGMRR
                     ELGFGGIGPRGMFFFYATVRSFAPRQILESGRMRGGSTLMLARCFPQSRIVSVELDRD
                     PTDAAIAEAKLKPYPNVELLYGDSREILPARLQPGDAVLIDGPKEFRALKFALRLLRT
                     GKPCAVLIHDFHQGQPARKFVERHWPGAFFSDDPTFAAHLRELNLRLGRNPDSKHAVA
                     PFVCLPADLPAPYSILLLRIVWARAVSTALGKITTLRRSCR"
     gene            complement(6898..8217)
                     /locus_tag="VE486_04830"
     CDS             complement(6898..8217)
                     /locus_tag="VE486_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZA38444.1"
                     /translation="MPSIKPSKLVIRSYQVGFGDCFLLTFRYPAAAGTFDDRHVLIDC
                     GSTGMPKKVGARLPAVAKAIAQDCGNKLTAVIATHRHADHINGFATSASGKGPGDILR
                     ALKTELVVQPWTEDPKVAKDARSPALRAVGSSKAFGPAKFVAGLNGMHEFAALILQDV
                     KRLGRALPARTAAQLNFLGEENLANLAAVKNLMTMGKKNEYLSYKNRTALARLLPGVK
                     IRVLGPPTPRQYPEIEKQRSKDNAEFWHIMGAAASHLTSPGGIAPFAARFAETSRSAA
                     VRWFIPRITAAHAEQRLELVRILDDAMNNTSLILLFQIGKMKFLFPGDAQIENWNYAL
                     KDAPDAAANRARLAKTDFYKVGHHGSLNATPKTLWNLFARKGPETKSDRLRTAVSTMA
                     GKHGSTSRQTEVPRRPLVEELAKNSKFFTTQTLKKGQFSEQFEFDDF"
     gene            complement(8204..10117)
                     /locus_tag="VE486_04835"
     CDS             complement(8204..10117)
                     /locus_tag="VE486_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38445.1"
                     /translation="MASELNLPSGNGKRLRPLPVPRIQKLTIIAQDPGVRDENGCILR
                     AAVELPAETLQPGPWGYRVHVIDYDAATSQLWKPYVYRMKNGILIDPFAKKSDAQLLS
                     DPRFHQQNVYAIIMRILARFEHALGRRVSWSFGGHQLKVAPHASCDANAFYSKQDEGL
                     LLGYFVDPSFKGKRPTAGNGLVFSCLSHDVIAHETTHALVDGLRRHYTDPSSPDQAAF
                     HEGISDIVALLSVFALEEVMSGLLSNLKDKKIVHERELTPERLRKSALFGLAEEMGSA
                     LAQVRGEALRRSVNLEPSAKWLRDPEFQEPHRRGEILVAAILNALIDIWAKRLDGLRR
                     TAEGKASLSRVVEEGRRVADTLLTMTIRALDYSPPVHLDFGDYLSALLTADREIRPDD
                     SVYRFRNALVESFARYGIKPSSPYGGNETGVWGSPEEETNQQLSYARSHFEPMQRDAD
                     EVFRFIWENREALDLYEGVYTQVQSVRPCVRIDEDGFTLRETVAEYVQILRLMPAELT
                     KAGYTQPDLAFLPDDREVALYGGGTLIFDEWGRLKYHIHNRLKDTKRQSERLQYLAEA
                     GYYLLRRPQTEAVRIGGQGHFARLHLNRGLNRSRKITEGWVTGIAPAPGQRSHLHEET
                     IAEESEPIEDAID"
BASE COUNT         2314 a         2846 c         2836 g         2226 t
ORIGIN      
        1 atcgtggagg ctcacggcgg tgaaatgcat gctcaatatg acccaaaagc ttcggcgctc
       61 gttacgacgc tcaccttgcc attgtgatcc tgtagccgcc tcgctgtgtc gaggcgctcc
      121 gcccgtggtc ccccagcacg gcgagacagc gccgtggcta caaatccgca aatccctgga
      181 atgaaaaccg agaaccttcg catcctgatc gttgacgatg aacgccctat tctcatgacg
      241 cttgaggcgc ttctcaagcg gcacggctat caagtcgata ctgcgcccac agcctcccag
      301 ggattaaagt tactgaaatc aaaatctccg tcgctcgttt tgctcgatct tcaattaccc
      361 gacgcggaag gactggaaat gctcgaccaa atcaagaccg agcggcctga gatgcaggtc
      421 attattttga cggcgcacga ttccttgaac aacgccatcg agtcgatcaa acgcggtgct
      481 taccacttta tcagcaaacc gtacgcgccc gaagaattgc tcagtctggt cgagaaagcc
      541 ctggaaaaac agtttctggt gcgagaagcc gaacaattgc ggcaaagaac agagcaactc
      601 gagaagcagt tggaaatcgc cgagacccgc gccacgccaa ttttcaatag caagcgaatg
      661 caagagatcg aggaattgat caacgcgatg gcgccgtcgg atgcaaatgt gctcatcgtc
      721 ggtgaaagcg gcgtaggcaa ggaagtgatc gccaacgcga ttcatacgcg cagccggcgc
      781 gctggaaaag aaatggtcaa actgaattgt gccgcatttc cacaaaccat gattgaggga
      841 gagctttttg gctatgtgaa aggagcgttt accggcgcga tgaacgactt tcctggaatg
      901 atcgcagcgg ctggtggcgg gacgcttttt ctggacgaaa tctcggatat gccgcctgat
      961 ctacagacac gttttttgcg cgtgctgcag gagcgggagt atcggccgct tgggagcacg
     1021 cgcaccatga aagctgacat tcgcgtgatc gcatcaacca atcgaccggt tgcgcaggct
     1081 cttttagagc accggttgcg ctccgattta tattaccggt taaacacttt tcaaattgaa
     1141 gtgcccgcgc tccgggagag gaagcaggac attccgcccc taattggtct gttcctaaag
     1201 cagtttgccc aacaactcgg aaagcccgaa ccagacatcg cgccggatgc gtttcaaaag
     1261 ttattggatt acgcctggcc gggaaacgtg cgggaattgc agaacgcaat tgaatatgca
     1321 gtggtgctgg cgcggcaggg attgatcgga gtaaaagagc tgcccgccga agttcaactg
     1381 ccaacagtgc tgcaactgac agagcgcgct ccggcagctg ccggacgaag cggcgtgcag
     1441 agtctcgatg atgtcgagag aaacacgatt ctccaggcgc ttgcagaatg ccacggcaac
     1501 aagaaaaagg ctgccgaacg cctcggcatc cagcgcgcca cgctctacaa caagatcaag
     1561 cgctacggaa tcgagatctg aaaagacccc gcgaacgacc cgaggaacat cacctcagct
     1621 cgaagcatca actgccaaac ttgactgctt aaagcagtcg agtttgagag gggtcgttca
     1681 tccattgagc ttcgctgcca acaggtctct tcagcacgcg cggcggcccc gcaaagcgtt
     1741 cggggctgtc ctcaacgggt gcccccttgt ggagcgtacg catcttgcgt gctcttttcg
     1801 gcatcctgcc gaaaagtttt tgtgtttggc aagatgccaa acacagcacg ctggaaacgt
     1861 gcgctaccca gacccaatgc actgaggaca gcgcacacta cagccaagtt gcgcgcatgc
     1921 tgccgagacg gagaagacgc agtcttctgg ggttctcggg acatgttttg cgctcattcg
     1981 tgtagttcgt gggcaattct gttcaggaca cattttggat tttcagtttt cgattttaga
     2041 ttaagcggca agagcaatct gcaatctaaa atccgaaatg gcgacatccc ggcatctcga
     2101 aaaagtcctg gcgcacgcgg cgaatcaact cgccgccacg ggagcgagac gcccgacaga
     2161 ggtcctcccg ctttacaaaa aatttctcaa tgtcgaggaa catcgcctgc gtctgaagca
     2221 ccaggcgggc ggaggtggcc gcgaaatttg cgcgcgacgc gccgaccttg ttgatgtttt
     2281 gctgcaatat cttttcggag ctgccgccac ggttactcgc ggaaacggcg agtcccgcat
     2341 cggcctggca ttgatcgctc ttggtggata tggccgcggc gaactcaatc cgttcagcga
     2401 catcgatgtc atgcttttgc acgaccaggg aagcaagaaa atctcaccgc atctcgaaga
     2461 gatggtgcag caggtgctct acccactttg ggacagtggc ttcaaggttg gtcattccac
     2521 gcgttcgatc aaagacgcaa tcgcgcaggc caatcgtgat atgcgcacaa aaaccgccat
     2581 gctcgagtca cgctttctcg ccggcgacga agagctggcg cgagaatttc gggaacaatt
     2641 tcgttcaaag tgtgtcgagg gatacgagcg ggattacgtc gaaatgcgca tgcaggatca
     2701 ggtagcgcgg cacaagaaat ttggcgacag cgtttacctc caggagcctc atgtgaagag
     2761 tggctgcggc ggactccgcg attaccaaaa tctcctttgg atgacgtatt tcaaagaggg
     2821 ctcactaagc acgaaccaac tggtgggtaa agattggctg agtgaaagcg accagcggca
     2881 aatcgagagg gcctacgagt ttctcctgcg cctgcggact gacctgcatt acgcgacagg
     2941 gcgcgccacc gacatcctgc acatgaactt gcaggaacaa atcgccgagc ggctcaatta
     3001 ttctgctcga aatggccagc ttcgcagtga aacgctcatg cgggattatt acgagcacac
     3061 gcgcaatatc tttcgtgtga cggagcgcat taccgagcaa tttgtcagcg ggcacgtcac
     3121 gagcaggacc cgttctctgt tcagttttct tccgttgata cgtccggaga aagttccgat
     3181 cggcgactct ttttttgttc gcaacaatca attgtatccg gcgcgacgcg atgtttttcg
     3241 caaagatccc gagcaaatga tgcgagcatt ccagtttgca caggaacgcg gcctggatct
     3301 aagtcccgaa ctggcggacc tcttgagccg cagcctcggc caggttaccc gcacttacca
     3361 gtatgcgcgc gggccgcgcg aaatctttaa gacgattctg tcccgaaaag gggaagtcgg
     3421 ccgcatcctt cgaatgatgc atcgggttga tttcctggga cgatacgttc ccgaattcgg
     3481 tcagctcacc tgtctggtcc aacatgagtt tatgcatcgt tacacggcgg acgaacatac
     3541 actggtgtgt atcgacaagc tcgatgctct ggcgacgacg gatgatcaga agttaatcgc
     3601 ttatcgcaga atatttgagc agctcgatga cccattcgtg ctttatctgg cgctgctttt
     3661 gcatgacagc ggcaaagccg ttggcgctcg gcgacactct gaagcaagcg cgctgttcgc
     3721 ccagcgcgtc gccgcccgcc tgcagttatc atccgagcag cgcaaatcgc ttattctgct
     3781 ggtcgatcac cacctcactc tctccatgat agcgcaacag agaaatctgg atgaccccgc
     3841 cacagtggca gaaatggccg gcaacgtgaa gaaccagaaa aacctgaacg cattgatgct
     3901 gcttactctg gccgacggtc aggggacaag cgccgaggca tggtcggatt ggaaagaatc
     3961 gcttgtctgg cagttgtttc acgagacctc ccgctatctg gcggaccggc aatcttacta
     4021 cgaacaaaca agaatcgagc gcgaaagcct acggcgcgcc gtggcgcagg atctttcgcc
     4081 cgattatgcg gatgaaatcg acgcgcactt tgatttcatg ccagacaatt attttcgcgg
     4141 cagagatgtc cccgaaattg tcgagcatct gaaattattc cgctcgttcg ttgaacaagt
     4201 cgccagttcg ccacaggacg gattcgctgc ggcgaaccgg ggcgggcatc cgctggctcc
     4261 tgcggttaaa tggaaggctc ttccagagca aggacacagt gttgtcagtt tctgcacgtg
     4321 ggaccgcgag cgattgctgg cgaaaattgc ggggtcattt tcggtcgtgc cgattaatat
     4381 tttgagcgcg gatgtttttc ccagggggga caatgtcgtg ctgagcgttt ttcgcgtgtg
     4441 cgatacgaaa gcaggcgctg tcaccgaccc gcgcgccttc gagctagtcg aacgaacttt
     4501 gcgcagcgca ctcgatgatg agcacttcga tttccttccg ttgatcgaaa aggcgaagcg
     4561 tcagatcccc caccgcgcgc cagggatcga attcccaacc cgcatttcca tggataacaa
     4621 aacgcatccg gcttacacgc tgattgagat ccaggctccc gaccggctcg gcttgcttta
     4681 cgatattctg acgtgccttg atcgggagag tgtttcgatc ccgctatctc gtattaacac
     4741 gcaggatggc gcggccatcg atactttgta tgtcgttgat cgttccaccg acgccaggat
     4801 taccgatccg gatcgcatca ggacgatcca cgaacatctt accagcacaa ttctgggtgg
     4861 cgccaccgcc aaagcaacgt aactaaaaag aacgggcttt accgtgtccg ccgctcattg
     4921 tccctgattc tatgaacgca ctaattctgg ctgcgggata cgctacgcgc ctttatccgc
     4981 tgacactcga taaacccaaa ccgctgctcg cggtcggggg caaaccgatc atcgaatgga
     5041 cgatggacaa cctcgcggat gttccagacc tggaaacgat ttatatcgtg acgaatgaca
     5101 agttcgcctc cgatttccaa gcgtggagcg agcgctatcg gagccggcac ccgaaattca
     5161 aattgaaaat cgtcaacgac ggttcccaaa gcgacgacga taagctgggt gcgattggag
     5221 atatcaattt cgttgttagc cgggaaaatt tgaccagcga cggcttgatt gtcattgcgg
     5281 gcgataactt gtttaccgaa tcgctcacgg gttttgtcgc ctgcgcgaag gaaaccgagg
     5341 caactgttgc cgtttacgac gtcggcgata ctgaggcgat caaggagtac gggaacatcg
     5401 ctgtggacgc tgacggaatc atcacgcatt tcgaagaaaa gccgcagaaa ccccggggga
     5461 ccctggccgc gatcgcactt tactattact cccccgaagt gctttcgctt ttaacaacct
     5521 atctcgccgc gggcaataat ccggaccagc cgggccgctt tgtgcaatgg ctttacacgc
     5581 gcaggcccgt gaagacattt cagatcaagg gccagtggct cgatatcgga agcaaagaga
     5641 tgctgaagaa agcagacgaa attttgtcta gagcctgatt gcgctcagaa tgcgccctga
     5701 ggcatccgcg tattaactat cgcatttaag tcgacctggt gcggtgattg ttcgacatcc
     5761 taaaggtgaa tattgcccgg cgagtccagt ctgcttggtc aaagataccc aaaccttccg
     5821 gtatttattg tgtgatgcgc gcgaacgagc gctttccctc gcgggtggag acagagctct
     5881 aggtagcgca cgcgtcgcgc gtgctggttt cggcgtcgcg ccgaaacagg cttttctgcg
     5941 gcatcgttgc gcctcgtgct gcgaagttta aacgaaagtc cgcgatcgcg aggacgcgtg
     6001 tgctacccgg agatcgcgtt gtcgcttgca ctccttcatg agcccacgct cgagattgtt
     6061 cgggaactca ggttgcgacc gccgagccga aacccgtttg ctagtaaatg cgctaacggc
     6121 aagatcgacg cagcgtcgtg atcttgccaa gtgctgtcga aaccgctcgc gcccagacta
     6181 tccgcaataa caagatcgaa taaggcgcgg gcaaatcggc cggcaggcac acaaaaggag
     6241 cgacagcgtg tttggaatcc ggattccgac caagccgcaa attgagttcg cgcaagtggg
     6301 ccgcgaacgt cggatcatcg ctaaaaaacg cgcctggcca atgtcgctcg acaaactttc
     6361 gcgctggttg accctggtgg aaatcgtgaa taagaaccgc gcatggtttc ccggtgcgca
     6421 gaagccgcaa ggcgaatttt agcgcgcgaa attcttttgg cccgtcaatg agaacggcgt
     6481 cgccgggctg gagtcgcgcg ggcaggatct cgcgggaatc accgtagaga agctcgacgt
     6541 tcggatatgg tttcagcttc gcctcggcga tggccgcatc ggttggatcg cgatcgagct
     6601 cgacgcttac gatccgagat tgcgggaaac agcgcgccag catgagcgtg cttccaccgc
     6661 gcatgcggcc tgattccaag atttgcctcg gagcgaacga acggactgtg gcgtaaaaga
     6721 aaaacatgcc gcgtggaccg attccgccaa atccaagctc acgtcgcatc ccagtgatcg
     6781 cgccgcaaaa atcggagagc aggtctccgg cttgctcgga aaccgtcgcc atgtttagcg
     6841 gagtttcatt tcccggcgct gcgattggcc gggcgattcc tttaaagcgc acttgcatca
     6901 aaaatcgtcg aactcgaact gctcgctaaa ctggcccttc ttgagagttt gagttgtaaa
     6961 aaatttggag tttttcgcca gctcctcgac gagcggacgc cggggcactt cggtttgtct
     7021 ggaagtgctg ccatgttttc ccgccatcgt ggaaacagcc gtccgaaggc gatctgattt
     7081 cgtttccgga cctttcctag caaagaggtt ccacaatgtc ttgggcgtgg cgttcaagct
     7141 gccgtgatga ccgaccttgt agaaatcggt tttggccaga cgagcccggt tcgccgccgc
     7201 gtccggcgca tctttcaagg cataattcca gttctcgatc tgagcgtccc ccggaaagag
     7261 gaatttcatt tttccaatct ggaataacag gatgaggctg gtgttgttca tggcatcgtc
     7321 gaggatgcgg acaagctcga gacgctgttc ggcatgcgcg gccgtgatgc gggggataaa
     7381 ccagcgaacg gcggcagacc tgctcgtttc cgcgaacctt gccgcaaagg gcgcgattcc
     7441 gcctggagag gtcaaatggc tggcggctgc gcccatgata tgccagaatt cggcgttatc
     7501 tttgctgcgc tgtttttcta tttccggata ctgcctgggc gtgggtgggc ccagaacacg
     7561 aatctttacg ccgggtagga gcctagccag cgcggttcta ttcttatatg acaagtattc
     7621 gttttttttg cccatggtca tgaggttctt gaccgcggcc agattggcga ggttttcttc
     7681 gccgaggaag ttcaattgag cggcagtccg cgcaggcaaa gcgcgcccca gccgttttac
     7741 gtcttgcagt atgagcgccg cgaattcatg cattccgttc aaaccggcga cgaattttgc
     7801 cggaccaaat gctttgctgc tgccaactgc gcgcaacgca ggcgaacgcg cgtccttcgc
     7861 aaccttcgga tcttccgtcc agggttggac aacgagctcg gtcttcaacg cgcgcaagat
     7921 atcgcccggg cctttgccgc tcgcggatgt tgcgaaacca ttgatatgat cggcgtgacg
     7981 gtgcgtggcg atcaccgcgg tcaatttgtt tccacaatcc tgcgcgattg ctttagcgac
     8041 cgcgggcaga cgcgcgccga ctttcttggg cattcctgtg cttccacagt caatcaggac
     8101 gtgacgatca tcgaatgtcc ctgcggctgc ggggtatcga aacgtgagga gaaagcaatc
     8161 gccaaatcct acctggtagg agcggataac caacttactc ggcttaatcg atggcatctt
     8221 cgatcggctc cgattcttca gcgatagtct cttcgtggag atgggacctt tgaccgggcg
     8281 ctggcgctat ccccgtcacc catccctcgg taatcttgcg cgagcggttc aagccgcgat
     8341 tgagatgcag gcgagcaaaa tggccttgcc caccaatgcg aaccgcttcg gtctgcggcc
     8401 ggcgcaaaag ataataacca gcttccgcca gatattgtaa tcgctccgac tgcctctttg
     8461 tgtccttgag gcgattatga atgtgatatt tcagccgtcc ccactcatca aagatcaggg
     8521 tgccgccgcc gtagagcgcg acttcgcggt cgtctggcaa aaaggccaaa tcaggttgcg
     8581 tgtatcctgc cttcgttagc tcggccggca tcaaccgaag aatttgcaca tactcagcca
     8641 ctgtttcccg cagggtgaag ccgtcttcat ctatgcgcac gcagggccgc acagattgca
     8701 cctgggtgta aacgccttca tataaatcga gcgcttcgcg gttttcccag atgaaccgga
     8761 acacttcatc ggcgtcccgc tgcatcggtt cgaaatgcga gcgagcgtag gagagctgct
     8821 ggttcgtttc ctcttcgggc gatccccaga cgccggtttc gttgccgccg tacggggaag
     8881 aaggtttgat gccataacga gcaaagctct ccactaaggc gttgcggaac cggtacaccg
     8941 agtcgtcggg gcgaatttcg cgatcggccg tgagcagggc actgagataa tcgccaaaat
     9001 cgagatgaac cgggggcgag taatcgagcg cccggatcgt catggtcagc aacgtgtcgg
     9061 cgacgcgccg accctcttcc acaacgcggg aaagactggc cttgccttct gctgtgcgcc
     9121 gcaacccatc gagacgcttt gcccaaatat caatgagcgc attcaaaatc gcggccacca
     9181 aaatctctcc ccgccgatgt ggttcctgaa attccggatc gcgcagccat ttcgcgctcg
     9241 gctcgaggtt aacggaccga cgcagagcct cgcctcgcac ttgcgccaga gccgagccca
     9301 tctcttccgc cagcccgaac aaggccgact tgcggagacg ctcgggagta agctcccgtt
     9361 catgaacgat tttcttgtcc tttagattgc tcagcaggcc cgacattact tcttctaatg
     9421 caaagacgga caggagcgcc acgatgtcgg agatgccctc gtgaaacgcc gcttgatccg
     9481 gcgacgaggg atcggtgtaa tgacggcgca ggccatccac caatgcatgc gttgtctcgt
     9541 gcgcgatgac atcgtgcgag agacaactaa agaccagccc atttcctgcg gtcgggcgct
     9601 tccctttaaa acttggatcg acaaagtatc caagaagaag accttcatct tgctttgaat
     9661 aaaaggcatt cgcatcgcag ctggcgtgtg gcgccacctt taattgatgg ccgccgaaac
     9721 tccagctcac tcgacggccg agcgcatgct cgaagcgtgc gagaatgcgc atgatgatgg
     9781 catacacatt ctgttgatga aaccgcgggt cgcttaataa ttgcgcgtcg ctttttttgg
     9841 cgaagggatc aatcaagatt ccatttttca tgcgatagac atacggcttc cacaactggc
     9901 tggtcgccgc gtcgtaatcg attacgtgca cgcgataacc ccatggcccg ggttgcaatg
     9961 tctcagctgg gagctccacc gccgcccgca aaatgcagcc attttcgtct cgcacgccag
    10021 ggtcctgagc gatgatggtt aacttttgga tacgcgggac tggcaaaggt cgcagccgtt
    10081 tcccgttccc tgacggaaga ttaagttcgc ttgccattcc gctaacagac catccttgca
    10141 tctatcttgt taagccgccc ctgccaaggt cgaaattcgt agcgagcgcc cgcaatggcc
    10201 gctggtgtcg cgagcacgtt ct
//
LOCUS       DASQUQ010000169         5089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_130626, whole genome shotgun sequence.
ACCESSION   DASQUQ010000169 DASQUQ010000000
VERSION     DASQUQ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130626"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VE486_04840"
     CDS             complement(<1..356)
                     /locus_tag="VE486_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38446.1"
                     /translation="MQRRLVLREFSLVIALVVICAFFAIVSPQFLSARNLSLLTTELS
                     ITATLAMGMLLVILPGNIDLSVGSGVGLLGGIASVLVFEHNWPAPLAMGVAFLTGLLI
                     WFCMGNLIVRQKIPAF"
     gene            complement(389..844)
                     /locus_tag="VE486_04845"
     CDS             complement(389..844)
                     /locus_tag="VE486_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZA38447.1"
                     /translation="MEQRTNSFKYARSFRDLVVYQKARRLARDVFNVSKRLPNAEAFS
                     LTDQWRRASRSIGGQIAEAWAKRHYVRHFASKLTDADAEQQETQHWIIISLDCEYITR
                     EEAKSLGSLCQEIGRMLNEMIEQADKFCGDRSSIIREGSTYVAESPPDY"
     gene            complement(875..2407)
                     /locus_tag="VE486_04850"
     CDS             complement(875..2407)
                     /locus_tag="VE486_04850"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZA38448.1"
                     /translation="MNPVVLQAENITKNFPGVVALKDVSFDLRQGEIHGLCGENGAGK
                     STLIKLVSGIYPHGSYEGDCFVHGQLAKFRSLADAQKAGIAVIYQELALVNDMTVGEN
                     IFLGSEPRTTGGLIDWHKVYHEARVLLRKFNVEIDPSEPVAKLGMGQKQLVEIVKALS
                     KDSNILILDEPTAALAEHEVLILLDILRDLRSRGIASIYISHKLDELFAVCDRITVLR
                     DGSSIATLDIKQANKAEVIKHMVGREISDLFPRRKSKLGPSALEVENLTVADPHSGKP
                     FLADISFSLRAGEVLGVGGLMGAGRTELLMHLFGAWGTRLSGSVHLNGREVRAREPQE
                     IIRHGMVMVSEDRRRYGLILDKTIGFNLSLSSLAKLTKNRLIDQGLEFQRNNHFFQSL
                     RMKAPTLEALVAKLSGGTQQKVVLGKALMTEPLVIFLDEPTRGIDVGAKLEIYETINQ
                     LTDAGKAVLLVSSELPELIGMSDRIVILHEGRIGGEFDRDEATQEKLLAAAMGQREVV
                     LT"
     gene            complement(2430..3497)
                     /locus_tag="VE486_04855"
     CDS             complement(2430..3497)
                     /locus_tag="VE486_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZA38449.1"
                     /translation="MKFRIFLVVASCLVSLVTGFVISRSGSSTESVRARAKRLIGLSM
                     DTLKEERWQGDRDLFVTRAREVGADVLVQSANSDDTRQISDVQALISRDVDVLVIVPH
                     NGGAMAKAVNMAHEAGIPVVAYDRLITDCDLDLYMTFDNEKVGELQAQFLVDHLPRDR
                     KKKIVRIYGAKTDNNALLYKRGQDKILSPLINKGEIEVVHEDWTQDWKPENAKKIASA
                     AITKAGANFDAILASNDGTAGGAIQALSEEGLAGKILVTGQDAELSACQRIVNGTQAM
                     TIYKPVSQLAKQAAEAATNMARGKPVFAPDKINNGKIDVPSIFLDVVVVTKENMMDTV
                     VKDGFHKAADMYRSLPAEPSP"
     gene            complement(3494..4657)
                     /locus_tag="VE486_04860"
     CDS             complement(3494..4657)
                     /locus_tag="VE486_04860"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HZA38450.1"
                     /translation="MKVFFCALLLIPVISIAADNDTPKFATANPSGRTAGGFDATAAE
                     RFAKLALACVGKEYPNKISHVLNSDADVAPPRKLTPAFYGCYDWHSSVHGHWLLVRLV
                     RTFPDASFAAPARDALRKSLTAENLKQEAAYLRGAGRASFERPYGLAWLLQLCAELRE
                     LDDAQAREISANLRPLEEAAVERLKTWLPKLSHPVRIGEHGQTAFGLGLMLDYARSMG
                     NEAFAKLVTDSARKFFLADKNCPLAYEPSGEDFLSPSLGEADVMRRVLSPAEFATWLK
                     DFMPQVPATPRHSEAATVDWLPVTVSPDRSDPKLAHLDGLNLSRAWMLEGIMSVLPAD
                     DARRPALTAAADAHRRAGLAAVTGEHYEGGHWLGSFAVYLTTQRGIQRGSKLK"
BASE COUNT         1077 a         1489 c         1360 g         1163 t
ORIGIN      
        1 atgaaagccg gtattttctg ccgcacgatc aagttcccca tgcaaaacca gatgaggaga
       61 ccggtgagga aagcgacgcc cattgccagc ggcgcgggcc agttgtgctc gaacacgaga
      121 acgctggcga taccgccaag aagaccgaca ccgcttccga ccgatagatc gatgttaccc
      181 gggagaatga cgagcagcat ccccatggca agcgtcgcgg tgatcgataa ctcagttgtg
      241 agcaacgaga ggttgcgcgc gctcagaaac tgcggcgaca cgatcgcgaa gaacgcacag
      301 attaccacca gcgcaatcac cagcgagaat tcgcgaagaa cgagacggcg ttgcattggg
      361 agagtgatcg gtaatcagtg gtcagtgatc aatagtcggg tggcgattct gcgacatacg
      421 ttgatccttc gcggatgatg gaggatcgat cgccgcagaa tttgtcggcc tgttcgatca
      481 tttcgttaag cattcgaccg atttcctggc agagcgatcc caatgacttt gcctcctcgc
      541 gtgtgatgta ctcgcaatcg agactgatta tgatccagtg ttgcgtttct tgttgttcgg
      601 catcggcatc ggtcagcttg ctcgcaaaat gccggacgta atggcgcttt gcccacgcct
      661 cagcgatttg tccgccgata ctccgggaag cgcggcgcca ttgatccgtc agcgaaaaag
      721 cttccgcgtt aggtaatcgc ttgctaacat tgaagacgtc ccgcgccagt cgccgcgcct
      781 tttggtacac gaccaaatcc ctgaaactcc tggcatactt aaatgaatta gttctttgct
      841 ccatgcgtta ttaattactg atcactgatt actcctatgt cagtaccacc tctctctgtc
      901 ccatcgccgc tgcgagcagt ttttcctgcg tcgcttcgtc gcgatcaaat tcaccgccga
      961 tgcggccttc atgcaggatc acgatgcgat cgctcatccc gatcaattca ggtagctcgc
     1021 ttgatacgag gaggacggct ttgcccgcgt cggttagctg attaatggtc tcgtaaattt
     1081 ccagcttggc gccgacatca attccccgag tcggctcgtc gaggaaaatt accagcggct
     1141 cggtcatgag cgccttgcca aggacaactt tttgctgcgt gccgccggag agcttcgcta
     1201 ccaacgcttc cagagtcggt gctttcatcc gcagcgattg gaagaaatga ttgttccttt
     1261 ggaattcgag cccttgatcg atcagacggt tcttcgtcag ctttgccaaa gatgacagcg
     1321 acaaattgaa gccgatcgtt ttgtccagaa tcaatccgta acggcgccga tcttcgctca
     1381 ccatcaccat gccgtgacga ataatttcct gcggctcccg tgctcgcact tcccggccat
     1441 tgagatggac ggagccgctc aatctggtcc cccatgcgcc gaacaaatgc atcagtagct
     1501 cggtgcgacc ggcccccatc agaccgccga cgcccagcac ttcaccggcg cgcaatgaaa
     1561 agctgatgtc ggcaagaaaa ggcttcccgg agtgaggatc ggctacggtg aggttttcaa
     1621 cttcaagggc tgatgggccc agcttcgatt tgcgtcgcgg aaacaggtcg ctgatttcgc
     1681 gcccgaccat gtgcttaatg acttctgcct tgttcgcctg tttgatgtcg agcgtggcga
     1741 tggaagatcc gtcgcgcagc acggtaatgc ggtcgcagac cgcaaagagc tcgtcaagtt
     1801 tatgcgagat gtagatgctg gcgattcccc gactccggag atcgcgcaga atatcaagga
     1861 ggatgaggac ctcgtgttct gccaacgcgg ccgtcggctc gtcaagaatg aggatgttcg
     1921 aatcctttga gagcgccttt acgatttcca ccaactgttt ctggcccatt ccaagcttcg
     1981 cgaccggctc gctcggatcg atctcgacgt taaactttcg taagagcact cgcgcctcgt
     2041 gatagacttt gtgccaatcg atcaaacctc ctgtcgtgcg cggctctgag ccgaggaaaa
     2101 tattttcgcc gactgtcatg tcattaacga gcgcgagttc ctgatagatg acggcaatgc
     2161 cggccttctg cgcatctgcg agtgagcgaa atttcgcaag ctgtccatgg acgaaacaat
     2221 ctccctcgta tgaaccgtgc ggataaatgc cggagaccag cttgatcagg gtcgattttc
     2281 cggcgccatt ttcgccacag agcccatgga tttcgccttg gcgcaggtca aagctgacgt
     2341 ccttgagcgc gaccacgccc ggaaaatttt tcgtgatatt ctctgcctga agcacaaccg
     2401 ggttcacgcg cggatccttt gaacggcaat cagggtgatg gttcagcggg gagactgcga
     2461 tacatgtccg cggctttgtg aaacccgtct ttcacaacgg tgtccatcat attttccttt
     2521 gtgaccacaa ccacgtcgag aaagatcgag ggcacgtcga tttttccatt gttgatcttg
     2581 tcgggcgcaa aaaccggttt tcctctcgcc atgttcgtcg ccgcctccgc ggcctgtttc
     2641 gcaagctgcg aaaccggttt gtaaatcgtc attgcctgag tgccgttgac gatccgctga
     2701 caagcgctca gctcggcatc ctggccagtg acgagaatct tgccggcaag tccttcttcg
     2761 ctcagtgcct gaatcgcgcc gccggccgtt ccgtcgttcg acgccagaat tgcatcgaaa
     2821 tttgcgcccg ctttcgtaat tgccgcgctc gcgatttttt ttgcgttttc aggtttccaa
     2881 tcctgggtcc agtcttcgtg cacgacctcg atctcgccct tgttgatcag cggactcagg
     2941 atcttgtctt gtcctcgctt gtagagcagc gcgttattgt ccgttttcgc accgtaaatg
     3001 cggacaatct ttttctttcg gtcccttggc agatgatcga ccaggaattg ggcctggagt
     3061 tcgcccacct tctcgttgtc gaacgtcata tagagatcga gatcgcaatc ggtgattaac
     3121 cgatcgtagg cgaccacagg aattcccgcc tcatgcgcca tgttgacagc tttcgccatc
     3181 gcgccgccat tgtgcggaac aatgacgagc acatcgacgt cccggctgat caatgcctgg
     3241 acgtcgctga tttgacgagt gtcatcgctg tttgctgatt gaaccaggac gtcggcgccc
     3301 acctccctcg ctcgggtcac aaacagatcg cggtctccct gccagcgttc ttctttcaac
     3361 gtgtccatcg aaaggccgat cagccgtttg gcgcgcgctc tgacgctttc ggtcgaggaa
     3421 ccgctgcgcg aaataacaaa gcccgtgacc aggctgacga ggcacgaagc gacaaccaga
     3481 aaaatcctaa atttcatttc aatttggagc cgcgttgaat cccgcgctgg gtcgtcagat
     3541 aaaccgcaaa acttccgagc cagtgtccgc cttcgtaatg ctcgccagtt actgccgcca
     3601 gtcccgcgcg acgatgagcg tcagcggcag ccgtaagcgc gggcctgcgt gcatcgtccg
     3661 cgggaagcac ggacatgatc ccctccaaca tccacgcgcg gctcaaattc aatccatcaa
     3721 gatgcgcaag cttcggatcg cttcgatcgg gtgagaccgt gaccggtagc caatcaactg
     3781 tagccgcctc gctgtgtcga ggcgttgccg gaacttgcgg cataaaatct ttcaaccatg
     3841 tcgcgaattc cgcaggcgac agcacccggc gcatcacgtc cgcttcaccg agcgaaggtg
     3901 agaggaaatc ttcaccagaa ggttcataag cgagcgggca atttttgtcg gcaagaaaga
     3961 attttctcgc cgaatcggtg accagtttgg cgaaggcttc gttacccatt gagcgggcgt
     4021 aatcgagcat cagaccgagc ccgaacgccg tttggccgtg ttcaccgatg cgcacgggat
     4081 gcgagagttt cggcagccat gttttgagcc gctcgactgc agcttcttca agcggacgca
     4141 ggttcgccga tatctcgcgc gcttgcgcat catccaactc gcgcagctcg gcgcacaatt
     4201 gaagcagcca cgccaatcca tatggacgtt caaaactcgc gcgacccgct ccgcgaagat
     4261 aagcagcttc ctgcttcaga ttttccgcgg tgagactctt gcgcagcgcg tcgcgcgccg
     4321 gcgcggcaaa cgatgcgtca ggaaatgtgc gtaccagcct gacgagcagc caatgtccat
     4381 gaacggatga atgccagtcg tagcaaccgt aaaatgcggg cgtgagttta cgcggggggg
     4441 caacatcggc atcgctgttc aacacatgac tgatcttgtt cggatattcc ttccccacgc
     4501 acgccagcgc cagtttggcg aatcgttccg cggctgtggc atcaaatcca cccgccgtgc
     4561 ggccggacgg attcgccgtg gcgaatttcg gagtgtcatt gtcagccgcg atgctgatca
     4621 ctggaatcaa tagcagagcg cagaaaaaga ccttcatcgt gctcggattt taggtctggg
     4681 gagcacaggc tgccagcctg tacgttttag ctgccagtcg aaactcactc tgtcgctcgc
     4741 caagccgatg ctgagcgaag gctggagtct tcgtataaat cccgcaaagc ggtggagagg
     4801 ccgtctcgtt cccgtagttg cgttcagcca cgccgcgtgc acaagtgaca ttctcactat
     4861 gcatcattct ctcgtgactc agctgttgcg catcttagca atcctcgccg ccatgcaaat
     4921 cacgtctgct tctctcaacg ccgggaaatc atcggcgaaa agctccccgt cgccgccatc
     4981 ttccggcgcc aatccgcttc tggcggaaag cactcttccg tatcacacgc cgccattcga
     5041 caagatcaag gacgagcatt tcgtcccggc gatcgacgcc ggcatgcgc
//
LOCUS       DASQUQ010000170         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_130953, whole genome shotgun sequence.
ACCESSION   DASQUQ010000170 DASQUQ010000000
VERSION     DASQUQ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130953"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /gene="tsf"
                     /locus_tag="VE486_04865"
     CDS             complement(<1..489)
                     /gene="tsf"
                     /locus_tag="VE486_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZA38451.1"
                     /translation="MNATTEIDPKLVKQLREKTNVGFMDCKRALVESGGDLEKAETIL
                     RTKGIATAAKKATRATKEGIVASYIHLQGKVGVLVEVNCETDFVARNENFREFVKDIT
                     LHIAAAHPLYVSRADVPGNLIEAEREIYKAQVQGKPAHVADKIVEGKLEKFYSAVCLL
                     EQG"
     gene            complement(546..1277)
                     /gene="rpsB"
                     /locus_tag="VE486_04870"
     CDS             complement(546..1277)
                     /gene="rpsB"
                     /locus_tag="VE486_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZA38452.1"
                     /translation="MADTTELVPELLEAGVHFGHQTKRWNPKMRPFIFEQRNQIYIIN
                     LDETVRQLRRATDFLQEVARRGGRILFVGCKKQAQEAIKEAALACGQFYVNQRWLGGT
                     LTNLATIRKSIGRLKYIEQIEQSPEYKKMGKQELAALNREAAKLRRNLEGILDMSELP
                     DVLVIIDTLREQNAVNEARRLGIPVVAIVDTNADPELVDYPIAGNDDAIRAIRVILQK
                     LVDAIVSASSEARIREQVEMAGVSA"
     gene            complement(1365..1826)
                     /locus_tag="VE486_04875"
     CDS             complement(1365..1826)
                     /locus_tag="VE486_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38453.1"
                     /translation="MKPFHRRLLYGVLALLFFSGAMWAYWNDLVTSPGDFEINAKAWA
                     MKIHGAAAMAILVLVGMLLTGHVRFAWRARRNRGNGSLFLGAFGILTVTGYGLYYAGG
                     ESLRSWTSWIHLAVGLALPLLLILHIWLGKRTRPAAQLQKRPPSVAEIDIA"
     gene            complement(1832..>2310)
                     /locus_tag="VE486_04880"
     CDS             complement(1832..>2310)
                     /locus_tag="VE486_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZA38454.1"
                     /translation="FGGIAKGFAVDRAVEALKRNRVMVGIVNAGGDLRVFGSAPQLVH
                     LRHPARPNRAAGAVKLRERAIATSGIYFARKEKQGRRASPMLDGRTGRAACDLISVTV
                     AAAKCMTADALTKVVFALRERAAPILTRYSADALLLEQDGGASWMFHPTCDTSRQN"
BASE COUNT          498 a          679 c          605 g          528 t
ORIGIN      
        1 gccctgctcg agcaggcaaa cggcactgta gaatttctcc agtttgccct cgacaatttt
       61 gtccgcaaca tgcgccggct tgccttgcac ctgtgctttg tagatttcgc gttccgcctc
      121 gatcaggtta ccgggaacat cggcgcggct gacgtagagc gggtgcgccg cggcgatatg
      181 cagcgtgatg tctttcacga actctcggaa attctcgttt ctcgcgacga aatcagtctc
      241 gcaattcacc tctacgagca caccaacctt gccctgcaga tgaatgtacg acgcgacgat
      301 gccttccttt gtcgcacgcg tggccttttt cgcagcagtg gcgatgcctt tcgtacgcaa
      361 gattgtttcg gccttttcca gatcgccacc actctccacg agagcacgct tgcaatccat
      421 aaatccgaca ttggtctttt cacggagttg cttgacgagt tttggatcga tttcagttgt
      481 tgcgttcatt gggtccttag aattgttgaa gcatccagag agctgaaccg ctgaagccgc
      541 taacgtcagg ccgacacgcc tgccatttcc acctgctcgc ggatgcgggc ttcactgctg
      601 gcggaaacaa tcgcatcgac cagtttttgt aaaataaccc gaatcgcgcg gatggcatcg
      661 tcgttccccg cgatcggata atcgacgagt tcgggatcgg cgttggtgtc cacgatggcc
      721 accaccggga tcccgaggcg gcgcgcctca ttgacggcgt tttgctctcg gagcgtgtcg
      781 ataatcacca acacatcggg caattctgac atatcgagga tgccctcgag gttgcgcctg
      841 agctttgcag cctcacgatt cagcgcagcc aattcttgtt ttcccatttt cttgtattcg
      901 ggcgactgct cgatttgctc gatgtatttc aatcggccga tgctcttgcg gatcgtggcg
      961 aggttagtca gcgtgccgcc cagccagcgt tggttcacgt aaaattgtcc gcacgccaat
     1021 gcggcctctt ttattgcttc ctgcgcctgt ttcttgcagc cgacgaacaa aatccggcca
     1081 ccgcggcgcg cgacttcctg gagaaaatcg gttgcccgac ggagctgcct caccgtttca
     1141 tcaagattga tgatgtaaat ctgattgcgc tgttcaaaga tgaaaggcct catctttgga
     1201 ttccaacgct tcgtctggtg tccaaaatga acaccagctt ccaataattc aggcactaat
     1261 tctgttgtat ctgccataag cgaaaattcc gggagaggga acttccagag agggacgttg
     1321 aagatgctat atcacgcgcc tgttcgcaac ctgaaattcg ctcgtcaggc tatgtcgatt
     1381 tccgccactg agggaggacg tttttgcagc tgagccgctg gccttgtccg tttcccaagc
     1441 caaatgtgaa ggatcaaaag caacggcagc gccaatccga cggctagatg aatccagctc
     1501 gtccacgaac gcaagctttc gccgccggcg tagtaaagcc cgtatccagt gacggtcaga
     1561 ataccgaatg cgccaagaaa cagcgacccg ttgccacgat tccgacgcgc ccgccaagcg
     1621 aagcggacgt gaccggtgag aagcattccg acaagcacca aaatcgccat cgcagcggca
     1681 ccgtggatct tcatggccca tgccttcgca ttgatttcga aatcacccgg tgacgtcacc
     1741 agatcattcc aatatgccca catcgcgccg ctgaaaaaaa gcagcgcaag cacgccgtag
     1801 agcaagcgtc tgtgaaacgg cttcaatcgg attaattctg gcgggacgtg tcgcacgttg
     1861 ggtgaaacat ccaggaggca ccgccgtctt gctcgagcag caacgcatcc gcggaatacc
     1921 gggttagtat aggcgcggct cgttcgcgca atgcgaacac aactttcgta agtgcatctg
     1981 ctgtcatgca tttcgctgcg gcgacagtca cactgatcaa gtcgcacgcg gctcggccgg
     2041 tgcggccgtc caacattggg ctcgcgcgac gaccctgttt ttctttgcga gcaaaataga
     2101 ttccggaagt tgctatcgcg cgctcgcgaa gtttcaccgc tccagcagcg cggttcggtc
     2161 gcgcggggtg acgaagatgc accaactgcg gcgccgatcc gaaaacacgc aggtcgccac
     2221 cggcattaac tatcccgacc ataacccggt tgcgtttcaa agcttcaacc gcccgatcga
     2281 cggcgaaacc tttcgcgatt ccgccgaaat
//
LOCUS       DASQUQ010000171         5300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1046687, whole genome shotgun sequence.
ACCESSION   DASQUQ010000171 DASQUQ010000000
VERSION     DASQUQ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046687"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VE486_04885"
     CDS             complement(<1..185)
                     /locus_tag="VE486_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38455.1"
                     /translation="MKKTAVVVLAACVVLATGCRWVGVRGNGNIKTEERTIDAFANIR
                     TSGAFEIEWQNGAPALR"
     gene            complement(350..1372)
                     /locus_tag="VE486_04890"
     CDS             complement(350..1372)
                     /locus_tag="VE486_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38456.1"
                     /translation="MSFVKKICAAVYFFSIASLLAAPIIPIAPGMTWRYNMTQEVDHG
                     ITVPDAKLDPDGKIRAPVVYRIEGTENVDGKDLLKFEMHRAGMITNTDLLTVNERGVV
                     CWARINLDGELVKFNPPQPMIAMPLKRGARWNFDGQAGDLKIRQHYEVTGEEDIQVPA
                     GKFQAFRIQGEQTSPSSLTIDRWFVPGTGIVKDVTTMRAKNGDLLERISLELAEPPRV
                     TDRPQVKPEAAPKKLSVSFAKEQAGKASTTFSADTPQIYARWQGERLRRGAKVRAVWI
                     AENIGEDFPPDYKVDEASAIAESPTAHGSFTLSRPEDGWAPGDYRAEFYVDDVLVETV
                     KLKIVE"
     gene            1503..3074
                     /locus_tag="VE486_04895"
     CDS             1503..3074
                     /locus_tag="VE486_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZA38457.1"
                     /translation="MLRIISLLLWIVCQAAIAAESTTPERHVVVIVWDGMRPDFVSEE
                     NTPALWKLAQEGVTFRNHHAVYPSATIVNGTAMVTGFYPAHSGLIANHEYRPEIDRKR
                     SIDVEVPAVIAKGDDLSGGKYISVPTIAELVQRAGRRTAIAATKTIGLLLNRHIDPGD
                     AKNYARVIAGKLPPLDALTSILNALGRFPSAYLQQDTWTTKALTDFLWKDGVPAFSLL
                     WLGQPDLTQHESAPGAPPALAAIKSADRNLEAVLSALDRPAFAKVTAGQASARATTDI
                     FVVSDHGFSTIKRSVDLRKILRDAGFSATTRFTDEPKRGDIMLVGNGGTVLFYVEGHD
                     AEVTRRLVEFLQQTDFAGVLFTKEPMQGTFSLDQATIDSKHAPDVVMAFRWNDSKNQF
                     GIPGMIDADWQRKAGEGTHATLGRFDMHNTLIAAGPDFRRGKMDDLASGNVDLAPTIL
                     RILEITPPRELDGRILSEAMVNIDMPDLKPETKTVEATKDFPSGSWRQSLQISRVGST
                     IYLDEGNGAFLPTTR"
     gene            complement(3061..>5300)
                     /gene="glgP"
                     /locus_tag="VE486_04900"
     CDS             complement(3061..>5300)
                     /gene="glgP"
                     /locus_tag="VE486_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZA38458.1"
                     /translation="KPIAYFSAEFGFHESIPNYSGGLGILASDHCKSASDLDLNFVGI
                     GLLYRHGYFRQQIDKEGVQEAISLNQSFYHLPIREVRRGDVTLLISVPMLGREVFAKI
                     WELCVGRVSVYLLDTDTPENSAEDRLLTAELYGGDLEMRMRQEIMLGIGGVKALNALG
                     IEPDVFHMNEGHSAFLALERIRLNIIQKKLDFYSALQVVAAANVFTTHTPIPAGNDSF
                     PREMMKKYFGTFATELNIPFDELFSFGQTRVDPNDPFSMTILALRLSRHANGVSKLHG
                     EVTRSLWKDVWAGVPVHEVPITSITNGVHTKTWMAPEFSALYQKHLGAWEEHLSEPDF
                     WRGVIDIPDAQLWETHQRLKLRLIEFVRELERRRRERLGESPEAIRRANRILDPEILT
                     VGFARRFATYKRATLLFSDKERLKRLLNDTTRPVQFIFAGKAHPRDEAGKALIQEVYK
                     FSREAGVENRIVFLEDYDSYIARRLVQGVDLWLNHPLRPLEASGTSGMKAAPNGGINL
                     SVLDGWWRESYNGNNGWAIGAEIDNGSAEFQNEVDASSLYQLLENQIIPLYYAKPDGK
                     LPLAWLQLMRESIRSVTPVFNTQRMVKEYTERLYVPAAQSYEDFSRDGCGAATQLSQW
                     KKQMRKDWPQVQISDIQIGNKDRQNISVGESLEITARVHLGAVAPNQVRVEAYHGEVD
                     NGDIRNPTATVLNQSSQADGDGNYIYQGSVPASESGTYGFSVRVVPTHPHLMQAHELR
                     LITWS"
BASE COUNT         1171 a         1465 c         1418 g         1246 t
ORIGIN      
        1 atacgcaagg caggcgctcc attttgccac tcgatttcaa acgcgccact tgtgcgaatg
       61 ttggcaaagg cgtcgatcgt gcgttcctcg gttttaatat tgccgttgcc tcgaactcct
      121 acccaccggc atccggtagc caagacgaca catgcggcca aaactacgac tgcggtcttc
      181 ttcatggaaa tatgcctcct gttgttagga tgtagggagc gtttgctttg gattcaagaa
      241 attctgcgtc gcaacgcttt tgtcatgtcg agcgaagtcg agacacctct cactgttaat
      301 gcttttgaat agtaagagat tcctcgactc cgctcggaat gacaaatatt cactccacaa
      361 tctttagctt caccgtttct accaggacgt catcgacgta gaactccgcc cgataatcgc
      421 cgggtgccca gccatcctct ggtcgagata acgtaaatga tccatgagcg gtcgggcttt
      481 cggcgatcgc agacgcttca tctactttgt agtccggcgg aaaatcctcg ccaatatttt
      541 cggcgatcca gacggcgcgc acttttgcgc ctcttcgcag acgttccccc tgccagcgcg
      601 cgtaaatctg aggcgtatcg gctgaaaatg tcgtgctcgc cttgccagcc tgctcctttg
      661 caaaacttac ggagagtttc ttcggggcgg cctcgggttt cacctgcggc cggtccgtaa
      721 ccctgggtgg ttcggccaat tctagtgaga tgcgttcaag caaatcgccg tttttggcac
      781 gcatggtcgt gacgtctttc actattccag tgccgggcac gaaccagcga tcgatcgtca
      841 gtgagctggg cgatgtttgt tcgccttgga tgcggaacgc ttgaaatttt cctgccggta
      901 cctggatatc ttcctcgccg gttacctcgt agtgttgacg gattttcagg tcgcctgcct
      961 gcccgtcgaa gttccacctg gcgccccgtt taagcggcat ggctatcatc ggttgcggcg
     1021 gattaaattt aaccaattcg ccatccagat taatacgcgc ccaacaaaca acaccgcgtt
     1081 cgttcacggt gagaaggtct gtgtttgtaa tcataccggc ccggtgcatt tcgaatttga
     1141 gaagatcctt cccgtcaacg ttttccgttc cctcaatgcg atacacgacc ggagcacgga
     1201 tttttccgtc aggatcgagt tttgcatctg ggacagtgat tccgtgatcc acttcctggg
     1261 tcatgttgta tcgccacgtc attccgggtg cgatggggat aatcggagcg gccagcagag
     1321 aagcgatgct gaaaaaataa acagcggcgc aaatcttctt cacaaaactc actcgcacgc
     1381 ttgcgcggtg ctaacaagga ggggcgccca gggagcagcg gtctctaggc cgccgctttt
     1441 tagccaggcg ttaattgaga cggcggactg gagaccgccg ctccttgttc tatgatttgc
     1501 gaatgctgcg aatcatctca ctcctcctat ggattgtctg ccaggctgcc atcgcggctg
     1561 aatcaaccac ccccgagcgg cacgttgttg taattgtctg ggacgggatg cggccggatt
     1621 tcgtgagcga ggaaaacacg cctgcgcttt ggaaactggc gcaagaagga gtcactttcc
     1681 gtaaccatca tgcggtttac ccaagcgcga caatcgtaaa cggaacggca atggttaccg
     1741 gattttatcc agcccacagc ggtctgatcg cgaaccatga gtaccgcccg gaaatcgacc
     1801 gtaaacggtc aatcgacgtc gaagttccag cggtgatagc gaagggggat gatttatcgg
     1861 gtgggaaata catttcggtt ccgacgatcg ccgagctcgt acaacgcgcg ggcagacgca
     1921 ccgcaatcgc tgcgacaaaa accattgggc ttttgctcaa tcgacatatc gatcccggtg
     1981 acgccaagaa ttacgccagg gttattgctg gcaaattgcc cccgctcgac gcgctgactt
     2041 ccattctcaa cgcgctcggt cgctttccgt ctgcgtattt gcaacaggac acctggacaa
     2101 caaaagcgct gaccgatttt ctttggaaag atggtgtgcc ggctttttcg ctcctttggt
     2161 tgggacagcc ggaccttacc caacatgaat ccgcgccggg cgcgccaccg gctctggccg
     2221 caattaaatc cgctgatagg aatctcgagg cggtgctttc ggcgctggat cgacccgcct
     2281 tcgccaaggt tacggcaggg caagcatcgg caagagcgac aacagacatt tttgtcgtct
     2341 ctgatcatgg cttttccaca atcaaacgat cggttgattt gcgaaaaatc ctccgcgatg
     2401 ccggcttcag tgcgacgacc agattcacgg atgaaccgaa acgtggagac atcatgctcg
     2461 tcggaaacgg cggtaccgtt cttttttatg tggaaggcca tgacgctgaa gttacgcgtc
     2521 gtctcgtcga atttttgcaa cagacggatt tcgccggagt ccttttcaca aaggagccga
     2581 tgcaagggac attcagtctc gatcaagcca cgatcgatag taaacatgcc ccggacgtgg
     2641 tcatggcctt ccgctggaac gattcgaaaa atcaattcgg tattccagga atgatcgacg
     2701 cggattggca acgaaaagca ggggagggga cgcacgccac gctcggccga ttcgacatgc
     2761 acaatacctt gatcgcggct ggtccagatt ttcgtcgcgg taagatggac gatttggcga
     2821 gcggcaatgt cgatctagcc ccgacaattc tgcgcattct cgagatcacg ccgccgcggg
     2881 aattggatgg cagaattctg tccgaagcga tggtcaacat cgacatgccg gacttgaaac
     2941 cggaaacgaa aacagttgaa gcaacaaaag atttcccgtc gggaagctgg cgccaatcgc
     3001 tccaaatctc acgagtcggc tcgacgattt acctcgacga aggcaacggg gcttttcttc
     3061 ctacgaccag gtgatcaaac gcagttcgtg cgcttgcatt aaatgcggat gcgtaggcac
     3121 cacgcggaca ctgaaaccgt aagtgccgct ttcggaagct gggactgatc cctgataaat
     3181 ataattgccg tccccatctg cctggctgct ttgattgagc accgtcgccg tgggattgcg
     3241 gatgtcgccg ttatctactt cgccgtggta tgcttccacg cgcacctggt ttggagcgac
     3301 tgcgcctaaa tgaacgcgcg cggtgatctc cagcgattcg ccgaccgaaa tattttgccg
     3361 atctttgttg cctatctgga tgtccgaaat ctgcacttgc ggccaatctt tccgcatctg
     3421 ctttttccat tggctcagtt gcgtggccgc gccgcagccg tcgcgagaaa aatcttcgta
     3481 cgattgcgcg gcgggaacat acaggcgctc ggtgtattcc ttcaccatcc gttgcgtatt
     3541 gaagactggc gtaacgcttc ggatcgattc ccgcattaat tgcagccagg cgaggggaag
     3601 tttgccgtcc ggcttggcgt aatagagcgg aatgatctgg ttctcaagca attgataaag
     3661 actactcgcg tccacttcgt tctgaaattc ggctgagcca ttatcgattt cagcaccgat
     3721 cgcccagccg ttgttgccgt tgtagctctc ccgccaccaa ccatcgagca cgctcaggtt
     3781 gatgccgcca ttgggagccg ccttcattcc actcgtgccg cttgcttcca agggacgaag
     3841 cggatggttc aaccaaagat caaccccctg cacaagacgg cgcgcgatgt agctatcgta
     3901 gtcttccagg aaaacgatgc gattttcgac cccagcttcg cggctaaatt tgtaaacttc
     3961 ctgaatcagc gccttcccag cttcgtcacg cggatgcgct ttgcctgcga aaataaattg
     4021 cacgggtcgg gtggtgtcat tgagcaatcg tttcagccgt tccttatcgc tgaacaagag
     4081 cgtggcccgt ttgtaagtcg cgaaacggcg cgcgaaaccg acagtcaaaa tttccgggtc
     4141 aagaatccga tttgccctgc gaatggcttc cggcgattcg cccaggcgct cgcggcgccg
     4201 tcgttccaat tcgcgcacaa attcaataag acgcagtttg agtctttgat gcgtttccca
     4261 aagttgtgca tccggaatat cgataacgcc gcgccagaaa tcgggttcgc ttagatgttc
     4321 ctcccatgca ccgaggtgct tctggtaaag cgccgaaaac tcgggcgcca tccatgtttt
     4381 tgtgtggacg ccgttggtaa tgctggtgat cgggacttcg tgcaccggca cgcctgccca
     4441 gacatctttc caaagcgatc gagtgacttc gccgtgcaac ttgcttacgc cgttggcgtg
     4501 ccggctcagg cgcaacgcga ggatggtcat gctgaacggg tcgtttggat cgacccgcgt
     4561 ctggccgaat gagaagagct cgtcgaacgg aatattcagt tccgtcgcaa atgtgccgaa
     4621 atattttttc atcatctcac gcgggaaaga atcgttaccg gcaggaatgg gggtgtgagt
     4681 agtgaagacg ttcgccgcgg cgaccacctg gagggcagaa taaaaatcga gtttcttctg
     4741 gatgatattg aggcgaatgc gctcgagcgc gagaaacgct gaatgccctt cattcatgtg
     4801 aaagacatca ggctcgatgc cgagcgcgtt aagcgctttc actccgccga tcccgagcat
     4861 gatttcctgg cgcatccgca tttcgaggtc gccgccataa agctcagcgg tgagcaatcg
     4921 gtcctcagcg ctgttttccg gcgtgtctgt atcgagaagg taaacgctga cccgacccac
     4981 acacagctcc caaatcttgg cgaaaacttc gcgacccagc atcggcaccg aaatgagcaa
     5041 ggtcacgtcg ccacgacgga cttcgcgtat cggcagatga taaaagcttt gattcagact
     5101 aatcgcttcc tgaacgcctt ctttgtcgat ctgctgccgg aaatagccgt gccggtagag
     5161 aaggccgatc ccgacaaagt ttagatcaag atcgctcgcc gatttgcaat gatcgctcgc
     5221 caggatgccg agaccgccgg aataattcgg aatcgactcg tgaaaaccaa attcggcgga
     5281 aaaataggcg atcggctttt
//
LOCUS       DASQUQ010000172         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_507379, whole genome shotgun sequence.
ACCESSION   DASQUQ010000172 DASQUQ010000000
VERSION     DASQUQ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507379"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..600
                     /locus_tag="VE486_04905"
     CDS             118..600
                     /locus_tag="VE486_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38459.1"
                     /translation="MAAPKQNLPQLYRFCFLMLGDSRKAQEVFQTTLREAALRAAHGE
                     LPNQRFWLFRDARWRCLETSETDLQAEPLQLEECDITSAAASQIAQLEPTQLAIWISG
                     APDPQRTALALFYLDEFDCREILELAELKLSELSRLLALGRRQFQGWLDAMLPEKTKP
                     "
     gene            681..1370
                     /locus_tag="VE486_04910"
     CDS             681..1370
                     /locus_tag="VE486_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38460.1"
                     /translation="MRSAILRASDWNGFANQQSFDRAVAELVRSIPIPPETAEWFPAK
                     DLISGSKWTWKKMVRNPAVLAVGIAVVVITGVLVFHVLGRLNDFPGSATARKLLTVAG
                     STRSVLLDPVNADAGTLSDLFFMKHGLEHYDVPVEFSGFRAIGSRVFDDDESRRIAQI
                     WVAEKRMQFFLFPAERDIKTGAVLRFPGWRYVRQEGWVGAVTEHNGVCFMAALRGREK
                     DLVPYIPKTKQ"
     gene            1511..1582
                     /locus_tag="VE486_04915"
     tRNA            1511..1582
                     /locus_tag="VE486_04915"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1543..1545,aa:Met,seq:cat)
     gene            1647..3173
                     /locus_tag="VE486_04920"
     CDS             1647..3173
                     /locus_tag="VE486_04920"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZA38461.1"
                     /translation="MNNENFFEKLFARGNSYWLTRFVILRLLGLVYAVAFLVAAQQLV
                     PLVGEHGLTPASHFLRVIQAQLGPQTAGMLRVPTLFWFGMSDNALSIFAWAGFGLSLV
                     VLGGYANAILLTILWAMYMSIVHIGQIWYGYGWEIQLLETGFLSIFLCPLIDGRPFPR
                     CRPPILVFWLFRWLGFRIMIGAGLIKLRGDSCWRDLTCLYYHYETQPIPNPISRYLHF
                     APHWFHKFGTAWNHFIELVVPWFSFGPRTARHIAGVLLVSFQIVLIISGSLSFLNYVT
                     IIPFLACFDDTFLRHFLPARLVRRAEQAAQESEPSRINNTVAIALSILVMYLSVAPVL
                     NLVSGRQLMNYSYDPFDLVNTYGAFGSVGRERPEIVFEGTEDALITGDTKWKEYEFKA
                     KPGDPNRRPPFVAPYQPRIDWQIWFAAMASPAEYPWTLHFVWKLLHNDRATLSLLANN
                     PFPDGPPHYIRARLYRYEFAPLDDKAWWKRQPIGDWLPELSTDDPEFRRVLRAMDWLD
                     "
     gene            3182..4252
                     /locus_tag="VE486_04925"
     CDS             3182..4252
                     /locus_tag="VE486_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZA38462.1"
                     /translation="MKKSVRVISANVLERIAEPASRFYPDYLAYRKREITWGELLARL
                     PHVAMIGDSVCTGVYVSSVLSTFWRARTCSGKNWFLDTCPSRAGIRSVSKRLEELTPF
                     VAMEYAGIGALVDHEDERQNFFRRILRTRNFSGQIGQLLATERFPDLVLIAIGHNNVD
                     WAWRCPSSELEQPENRLQRQSRRFRENFARQMRRLIESARMQRHRVAAVVYGLVNFEV
                     YFKGRESAERLRARDPARYPHLETTYKYFTSFRPGYRSNLIRLTALVNEELRAMVSEL
                     NGELAGNVNLQVRYSDALATADLSRAELLHPVDGWHASIKGHNVLADAAFSDLGPTLE
                     FLGIKSPQGGAVSNPPIWGTAD"
BASE COUNT          884 a         1198 c         1244 g         1094 t
ORIGIN      
        1 gtagcagcct cgctgcacga ggtgcatacg cccgttcgcg cgcctgcaac gaagatgtcc
       61 aatcctccct tgtcgaccga gtcgaggcgc gtaacgttcg ggttttgaaa aaccagagtg
      121 gccgctccaa aacaaaatct accgcagctt tatcgctttt gttttttgat gctgggcgat
      181 tcgcgcaaag cacaggaagt tttccagacg accttgcgtg aagcggctct gcgcgcagct
      241 cacggggaac tgccaaacca gcgattttgg cttttccgcg acgcacgctg gcgttgtttg
      301 gaaacaagcg aaaccgactt gcaagccgag cctcttcagt tggaggagtg tgatattact
      361 tcggcggctg cgtcacaaat tgcacagctc gagcccacac agctggcaat ttggatttcc
      421 ggtgcgccgg atcctcagag gaccgcgctt gccctttttt atctcgatga attcgattgc
      481 cgcgaaattc tcgagctggc ggaactcaag ctgagcgagc tttcgcggtt attggcactg
      541 ggacggcgac aatttcaagg ctggctggat gccatgcttc ctgaaaagac gaaaccatga
      601 agactccgcg cttatcgcgt cggaagcgag cgctcctgaa ttgcgcgccg gtcggcgagg
      661 agcgcatccg cgaccgttcc atgcgttcag ccatcctccg tgcgagcgat tggaacggat
      721 ttgcgaatca acagagtttt gatcgcgcgg tcgccgaatt ggttcgtagc atccccattc
      781 cgccggagac tgcggaatgg tttccggcca aagacctgat ttctggctcg aaatggacat
      841 ggaaaaagat ggttcgcaat ccggccgtgc ttgcggttgg aattgctgtc gtggtcatca
      901 ctggtgtgct tgtcttccac gttcttggtc gcctgaacga ttttccagga tcagcgaccg
      961 cgcggaaatt attaacggtt gctggctcga cccgctcggt ccttctcgat ccggtgaacg
     1021 ccgacgcggg cacgttgagc gatctgtttt ttatgaaaca cgggctggaa cactacgacg
     1081 tgcccgtaga gttctctggt tttcgcgcca tcggctcgcg cgttttcgac gatgacgagt
     1141 cgcgacgcat cgcgcagatt tgggtagccg agaaacggat gcagttcttt ctttttccgg
     1201 cggagaggga catcaaaaca ggcgcagtgt tacgatttcc cgggtggcgc tatgttcgtc
     1261 aggaaggctg ggttggagct gtcacggagc ataatggggt gtgtttcatg gccgcgcttc
     1321 gtggccgcga aaaagacctc gtcccgtaca ttccgaagac gaaacagtaa ttcaagctcc
     1381 gttgacatgt cgtgctctgc cattgaatct tttcgcctca acgccaggta gctcagcggt
     1441 agagtagccc cgaaagcatt cggggttggt cggattgggt atgcttggta ttcttacaac
     1501 caaaattaga gccagggtag ctcagcggta gagcagggga ctcataagcc cttggtcggg
     1561 agttcgattc tcccccctgg cattccctgc ctgggcgttg gacgttgagc gttcggcgtt
     1621 ggacgtttcc ggaaattcgt gccgctgtga ataatgagaa ttttttcgag aagcttttcg
     1681 cacgcggaaa ttcctattgg ctgacgcgat tcgttattct gcgactgctg ggtctcgttt
     1741 atgcagttgc gtttctggtt gctgcccagc aactcgtccc actggtaggc gagcacggat
     1801 taacaccagc aagtcatttt cttagagtta ttcaagccca gctcggtccg caaacggccg
     1861 gtatgttgcg agtcccgacg ctcttttggt tcggaatgtc agacaacgca ttgtcgatct
     1921 ttgcctgggc aggtttcggt ttgtcgctgg tcgttcttgg cgggtatgcg aacgcgatcc
     1981 ttctcacgat tctctgggcg atgtacatgt cgattgtcca cattggccaa atctggtacg
     2041 gctacggctg ggagattcaa ctgctcgaga ccgggttttt gtcgatcttt ctctgcccat
     2101 tgatcgatgg gcggcctttc ccgagatgcc gtcctccgat tctcgttttc tggctttttc
     2161 gctggctcgg atttcgcatc atgattggcg ccggtctgat caaattgcgc ggcgactcct
     2221 gctggcgcga tctgacgtgc ctttactatc attacgaaac gcagccaatc cccaacccga
     2281 tcagtcgcta tttgcatttc gcaccccatt ggtttcacaa attcggcacc gcatggaacc
     2341 attttatcga gctggttgtg ccctggttct cattcggtcc gcggacggca cggcacattg
     2401 cgggcgtcct gctcgtcagc tttcaaatcg ttctcataat cagtggcagc ctctcctttt
     2461 tgaactacgt caccatcatt ccgtttctcg cctgttttga tgatacattt ctacgccact
     2521 ttctgccggc gagactcgtc aggcgcgctg aacaagcagc tcaagaatcg gaaccgtcac
     2581 gcatcaacaa cacggtcgcg atcgcattgt cgatcctggt gatgtatctg agcgtcgcgc
     2641 cggtgctcaa tcttgtttcg ggccggcaac taatgaacta ctcatacgat ccgttcgatc
     2701 tcgtaaatac ttatggcgcg ttcggcagtg ttggccgcga acgccccgaa attgtttttg
     2761 aaggaaccga ggatgcattg atcacaggcg acaccaaatg gaaggaatac gaattcaaag
     2821 ccaagccggg cgatccaaac cggcgcccgc cgttcgtcgc gccgtatcaa cctagaatcg
     2881 actggcaaat ctggttcgcg gccatggcgt caccggccga atacccgtgg acactgcatt
     2941 ttgtctggaa gcttctgcac aatgatcgcg ctacgctctc gctcctggcg aacaatccat
     3001 ttcccgacgg gccgcctcat tacattcggg cacgacttta tcgttatgag tttgctccgt
     3061 tggacgacaa agcatggtgg aaacgccaac cgatcggtga ctggttgccc gaactttcga
     3121 cggatgaccc ggagttccgt cgtgtgctca gagcgatgga ttggttggat tgactgaatt
     3181 catgaagaaa tctgttcggg tgatcagtgc gaacgttctg gaacgtatag ccgagccagc
     3241 gtcacggttt tatcctgatt atctcgctta caggaagcgg gagattacct ggggtgaatt
     3301 gttggcgcgg ctgccgcacg ttgccatgat cggcgacagc gtttgcaccg gcgtgtatgt
     3361 ctcgtcggtt ctgagcacct tttggcgagc acgcacttgt tctggcaaga actggtttct
     3421 cgacacttgc ccctcacgcg cgggcattcg gagcgtttcg aagagactag aggagcttac
     3481 gccgtttgtt gcgatggaat atgcgggcat cggcgcgctc gtcgatcacg aggacgagag
     3541 gcaaaatttt tttcggagaa ttctgcggac gcgcaatttc tccggacaaa ttggccaatt
     3601 gctggcgacg gagcgatttc ccgatctcgt tctgatcgcg atcggacaca acaatgtcga
     3661 ttgggcgtgg cgatgtccat ccagcgaatt ggagcagccg gagaatcgct tgcagcgaca
     3721 gagccggcgc tttcgagaaa atttcgcgcg acagatgcgt cgactgattg aaagcgcgcg
     3781 aatgcagcgt caccgggtcg ctgccgtggt ctacggtctc gtcaatttcg aggtgtactt
     3841 caaagggcgc gaaagcgccg aacgtctgcg agcacgggac ccggcccggt atccgcacct
     3901 tgaaactacc tacaagtatt tcacatcctt ccggccaggt taccggtcta atctgattcg
     3961 tcttaccgcg ctggtgaatg aggaactgcg cgcgatggta agcgaattga acggcgagct
     4021 tgcgggcaat gtcaatctac aagttcgcta ttcggatgcc ctggccacag ccgatttgag
     4081 ccgcgcagaa ttgctccatc cagtggacgg ctggcatgcg tccattaaag gccacaatgt
     4141 gcttgcggac gccgcgttta gtgacctcgg gccaactctg gaatttcttg gaatcaaatc
     4201 gcctcaagga ggggcggttt ccaatccgcc catttggggg acggcggact agagaccgcc
     4261 gctccttggt ggcaaatctc cgcgctcctt cagttgcgcg attgtctctc ggatcatccg
     4321 gtaaccgaaa tggccctggc ggacgcgcag caattccgac ccgcgccact tctgctgaat
     4381 ttcctcgata tggtcgggtc gcgcgatcaa atcgaattcg
//
LOCUS       DASQUQ010000173        10502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_400700, whole genome shotgun sequence.
ACCESSION   DASQUQ010000173 DASQUQ010000000
VERSION     DASQUQ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400700"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VE486_04930"
     CDS             <1..613
                     /locus_tag="VE486_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZA38463.1"
                     /translation="LVTRGVGNLGLNPAQCKYPSVIIIAATIALYHESFYRKGLTIVT
                     CATRRSNPAALNPAIKSLNYLNNVMARIEANLAGADEALMLNDAGNVAECTADNVFIV
                     KRGQIFTPPITAGALRGITRGIVFEIAGELGIKVSEADITRHDVFVADECFLTGTAAE
                     IVPVVKADGRVIGNGKPGPITTRTIARFREMTRETGTPIFAES"
     gene            762..1193
                     /locus_tag="VE486_04935"
     CDS             762..1193
                     /locus_tag="VE486_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HZA38464.1"
                     /translation="MQKVNLCDACSKEKGVQDPTGFALADLLLGIGAAEEIEKGASTQ
                     KCPVCGFSQADFKKTGRLGCSVCYVTFAEGLNTLLKAMHKGTEHVGKLPERAHRAIEL
                     HHQMRALTENLQKAVSEENYETAASLRDQIKQLETDLPNSP"
     gene            1190..2308
                     /locus_tag="VE486_04940"
     CDS             1190..2308
                     /locus_tag="VE486_04940"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HZA38465.1"
                     /translation="MKLSSMTATAGEWLRGEGPHHQIVVSSRVRLARNLRNRPFPGWA
                     KKAERGSILELIRPRVEDLPEMQDSFSESLQDLSALDRQVLVERHLISREHAAKGAGS
                     AVVVNRRQTLSIMINEEDHLRMQSIRSGLQLKQAFKLVDKVDSALESKLEFAFDPRLG
                     YLTACPTNVGTGMRASAMLHLPGLVLSELINQVIQAVSKIGLAVRGLYGEGTEAMGNL
                     FQISNQTTLGEKEEEIINRLSKVIETIIEKEHDARQVLLQKKPNTLLDQIGRAYGVLT
                     YAHAMASKEALNLLSIIKLGVDLGAFPEDRRLPVDELFIDTQPAHLQKSSQQKLNAEE
                     RDHLRAQIIRERLKLFPKPDISKMARKAGNGSTAEAIE"
     gene            2308..4785
                     /locus_tag="VE486_04945"
     CDS             2308..4785
                     /locus_tag="VE486_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HZA38466.1"
                     /translation="MNNFTPRAQQVLALARKEADRFNHNYVGTEHLLLGLIKLGQGVA
                     VNVLQKMGLDLETVRMEVEKQVGSGPETKMVGNVPYTPRVKKVLALAGKEAKALNHSY
                     VGTEHILLGLLREGEGVAARVLKSLEVDIERTRNEILKELDPNFTPPESDQESGEPAR
                     KDVKTPALRAFGRDLTDLAKKGELDPVIGRHNEIERVIQVLCRRTKNNPVLIGEAGVG
                     KTAIAEGLAQEIANGNVPELLHDKRVITLDLALMVAGTKYRGQFEERIKAVMDEIRRS
                     KNVILFIDELHTIVGAGSAEGAMDASNIIKPALSRGELQCVGATTMNEYRKYIEKDAA
                     LERRFQTIKVDAPTVDEAIQILKGLRPKYEAHHKAKLTDEALETAVRFSDRYITGRFL
                     PDKAIDVMDEAGARARINAMTRPPDVKEIEKEIEEIRLEKEAAIKAQDFEKAAALRDK
                     EKQTKEKLDTILARWREEREEREVVVTADDMMHIISKVTGVPLQRMEQEETQKLLMME
                     SEMKQRVIGQDEAVTAISKALRRSRADLKDPRRPIGSFVFLGPTGVGKTYLARTLAEF
                     MFGDADALIQIDMSEYMEKFTASRLIGSPPGYVGYEEGGQLSEAVRRRPYSVVLFDEI
                     EKAHPDVMHLLLQILEDGKITDSLGRKIDFRNTIIIMTSNVGADLLKKQTVMGFGAPL
                     EGHDYVSMRDKILDETKRVFKPEFLNRLDEIIVFHSLGKPELLRIVDLEVDKVLTRIK
                     GKDVQIELSQSAKEFLIEKGYDPQYGARPMRRGVERYLEDPLAEELLRGHVKAGDRVE
                     VVAAEGRLTFHVAESQPQSSTAAPTTG"
     gene            complement(4802..5656)
                     /gene="xerA"
                     /locus_tag="VE486_04950"
                     /pseudo
     CDS             complement(4802..5656)
                     /gene="xerA"
                     /locus_tag="VE486_04950"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
     gene            complement(5814..6317)
                     /locus_tag="VE486_04955"
     CDS             complement(5814..6317)
                     /locus_tag="VE486_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38467.1"
                     /translation="MRRAVHLLLASPAAAGLLGALFLNGCQTVPQGIQQARIEMAQRI
                     TTEQPGDYYIGRRYYKPDYKFWGYVRRPGQPWSTAQLVMLNEKQKLAPDREQLEFGND
                     NNYEYKLYGEFSGDKVYEPASNSVYPEFVLKGYELISTNPAPIFKSQFRGTGTPPELR
                     YAVEKPE"
     gene            6467..7264
                     /locus_tag="VE486_04960"
     CDS             6467..7264
                     /locus_tag="VE486_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZA38468.1"
                     /translation="MMVRFFNALGTPLLAFVQYLGEVVLLAADTFRCIFTHRLRWKLF
                     LNQVVEIGLLSQLVVVITGAFTGAVFSAQTFFQFNKLGMGSAVGAVVSVSLCRELAPV
                     LTALMVAGRVGAAMSAEIGTMKVTEQIDALRALAVYPVDYLVVPRALAMMVSMPLLVA
                     ECIAVGIIAGYFVAIFMLDINGTYYMANMERWTRSRDIIMGLSKAFCFGILIVFISCH
                     KGLTAREGAVGVGRATTEAVVNSSLAVLICNFFLTMLFNIIYPAGYQ"
     gene            7426..8142
                     /locus_tag="VE486_04965"
     CDS             7426..8142
                     /locus_tag="VE486_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZA38469.1"
                     /translation="MTKKIDRQEILRGVDLEVARGETLAIIGRSGGGKSVLLKHLVGL
                     MRPNAGEIWIEGQNITKMNERQLGAIRQKVGILFQGGALFDSMTVEENIAFPLREAGE
                     RDPKVLRAKVNEMLEVMEMEGEQSKMPVSLSGGMKKRVGLARSIIRRPSCVLYDEPTS
                     GLDPVVADSINRLIRRLQERFGVTSIVVTHDMKNAVHVADRIAYLHEGRIYFHGTSAE
                     LQKSSDPLIQDFLLGRSDGQ"
     gene            8191..9078
                     /locus_tag="VE486_04970"
     CDS             8191..9078
                     /locus_tag="VE486_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZA38470.1"
                     /translation="MNRHERGLEFKVGIFVFVGLAMLGALVVQFGRLGEGFKTYYALT
                     VRFNDASGLLKGSDVLLAGARIGKVADGPRLVEGGGIAVPLKIYDYIKIPEGAKFTVG
                     STGLLGDRFVNVTMPPGQPKAYLPPNASISGARETGIDDLTREGGALVGDLRGTVQKI
                     DTTVNRLNQDTLSSANMENLKSSMEHLNQATGALAESSKKLDGVIEQADSTMTSAKEA
                     ADNLQNAIADARKVLSSATKGKGLVAALLNDQQLANDLHALITNLRTHGVLFYRDSAA
                     KIEAKAQEQTKPARRTRGR"
     gene            complement(9121..9522)
                     /locus_tag="VE486_04975"
     CDS             complement(9121..9522)
                     /locus_tag="VE486_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZA38471.1"
                     /translation="MITGTHVLLYSKDPEADRAFFRDILNFRSVDAGGGWLIFKLPPA
                     ELGIHPSMDNFVQTHAEHSLLGSVLYLMCDNLDEVIKKLKSKNVSCTAIETAEWGITT
                     SVKLPSGGEIGLYQPTHPTAIDSGLTQDLET"
     gene            complement(9677..9826)
                     /locus_tag="VE486_04980"
     CDS             complement(9677..9826)
                     /locus_tag="VE486_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZA38472.1"
                     /translation="MATLPVRIVPNAKANQVIGAHGAAIKITLRAPAVEGKANAALRS
                     FWPKN"
     gene            complement(9861..>10502)
                     /locus_tag="VE486_04985"
     CDS             complement(9861..>10502)
                     /locus_tag="VE486_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38473.1"
                     /translation="ARGMAATVGLFNYPVLMAADILIYDSDIVPVGKDQKQHIEITRD
                     LAVKMNERFGSEPDGRIFKLPEPRIQAATEVVPGIDGQKMSKSYGNNIDIFGNEKETR
                     KRIMSIVTDSTPVEAPKDPSRSIIFRLYSLVASKNEIAEMRERFQKGGNGYGDFKKQL
                     FEKLWDYFAPMRKRREEILADKSYIDNVLARGAKRANEVADKVMERVRAAVGL"
BASE COUNT         2457 a         2725 c         2985 g         2335 t
ORIGIN      
        1 gttggtcaca cgcggagttg gcaatctggg attaaatccg gcgcaatgta aatacccgag
       61 cgtgattatc atcgcggcga cgatcgctct ctatcatgaa agcttttatc gaaaaggatt
      121 gacgatcgtg acctgcgcaa cgcgccgcag taatccggcc gcgcttaatc cggccatcaa
      181 atcacttaat tacctgaaca atgtaatggc gcggattgaa gcgaacctgg ccggcgcgga
      241 cgaggcgctg atgttgaacg atgcggggaa cgtcgccgaa tgcactgctg ataacgtttt
      301 tatcgtcaag cgcggtcaaa tctttacgcc acctatcaca gccggggcgc tgcgcggcat
      361 tacgcgcggg atcgtattcg agatcgctgg cgaactcggc atcaaagttt cggaggcaga
      421 tatcacgcgc catgacgtct tcgtggccga cgaatgtttc ctcacaggga ctgcggcgga
      481 gatcgttcca gttgtcaaag ctgacgggcg cgtgatcggc aacggaaaac cggggccaat
      541 caccacgcgc acaatcgcac gattccggga gatgacgcgt gaaacaggca caccgatttt
      601 tgcggaaagt tgagcagaga cggttccgaa gtggaaagtg cccggaaata ttcaggtttg
      661 aatgtatcca aactttctag gtctaattag cagagatgtt gtgtgacgtt tgcaaatgca
      721 atgacgcgac ggtcttcctg acgcaaattc tggagggtaa gatgcagaag gtgaatctct
      781 gcgacgcttg ctcgaaagaa aaaggagtgc aggaccccac cggtttcgcg ctcgccgatt
      841 tgcttctcgg catcggtgca gcggaggaaa tcgaaaaagg agcatcgact caaaaatgtc
      901 cggtgtgcgg tttcagccaa gccgatttca agaaaactgg ccgtctcgga tgcagtgtgt
      961 gctatgtcac gtttgccgaa ggcttgaaca cgcttttgaa agcgatgcac aaggggaccg
     1021 aacatgtggg caaactgcca gaacgcgcgc atcgcgccat cgagttgcac caccagatgc
     1081 gtgccttgac tgagaacttg caaaaagcag tctcggagga gaactacgaa acggcggcat
     1141 cactgcgcga tcaaattaag caactcgaaa ctgatctccc gaattcgcca tgaaattgtc
     1201 cagcatgacg gccacagccg gtgaatggct gcgcggtgaa ggacctcatc accaaatcgt
     1261 ggttagcagc cgcgttcggc tggcacgcaa tttgcgcaac cggccgtttc caggttgggc
     1321 aaagaaggca gaacgcggtt cgattctcga attgatcaga ccgcgcgtgg aagatcttcc
     1381 tgaaatgcag gattcatttt ccgaaagcct gcaggatctt tcggcgctgg accggcaggt
     1441 ccttgtcgag cgccacctga ttagtcgcga gcatgcggcc aagggcgccg gtagcgcggt
     1501 tgtggttaat cgccggcaaa cccttagcat catgatcaat gaagaagatc atctgcgcat
     1561 gcaatcgatc cgctctggtt tacagctaaa acaggcattt aaactggtcg ataaggtcga
     1621 cagtgcgctc gaaagtaaac tggagttcgc ctttgatccg cgtctgggct atttgactgc
     1681 ttgtccaact aacgtcggca ccggcatgcg cgcgtctgcg atgttgcatc tgcctgggct
     1741 cgtcttgagc gaactgatta atcaggttat tcaggcggta agcaaaatcg gtctcgcggt
     1801 ccgcggcctt tacggcgaag ggaccgaagc gatgggcaac cttttccaga tttccaatca
     1861 gacaacgctt ggtgaaaaag aagaggaaat cattaatcgt ctgagcaaag tgatcgaaac
     1921 aattatcgag aaggaacatg acgcccggca ggtgttgctc cagaaaaaac cgaatacact
     1981 tttagatcag atcggccggg cctatggcgt tttgacttac gcccatgcaa tggcttccaa
     2041 ggaagcgctc aatttattgt cgatcatcaa actcggcgtc gatctgggcg catttccgga
     2101 agatcgacgt ttgcctgttg acgaattgtt catcgatacc cagccggcgc acttgcagaa
     2161 aagctctcag cagaaattaa atgcggaaga gcgcgaccat ctgcgcgccc aaattattcg
     2221 cgagcgtttg aaactcttcc ccaaacctga tattagtaag atggcaagga aggcgggcaa
     2281 tggctcgact gccgaagcca ttgaataatg aataatttca cgccgcgtgc tcaacaagtc
     2341 ctggcgcttg ctcgcaagga agccgatcgc tttaatcaca actatgtcgg caccgagcat
     2401 ctgctgctgg ggctcatcaa actggggcag ggggtagcgg taaatgtttt gcagaaaatg
     2461 ggtctcgatc ttgagaccgt gcgcatggaa gtcgagaagc aagtcggctc cggcccggaa
     2521 accaagatgg tcggcaatgt tccgtacacg ccgcgagtta agaaagtcct cgcgcttgcc
     2581 ggcaaggaag ccaaagcgct caatcattcc tacgtcggca cggaacacat tttgcttggc
     2641 ctcttgcggg aaggggaagg cgtcgctgca cgagtgctca aaagtctcga agtcgatatt
     2701 gaacgcaccc gtaacgagat cctaaaagag ctcgatccaa atttcacacc gcccgaatcg
     2761 gaccaggaga gcggcgaacc agcaagaaaa gacgtcaaga ctcccgcgct ccgagcattt
     2821 ggccgtgatc ttaccgattt ggccaagaaa ggggaacttg atccggtgat tggccggcac
     2881 aatgaaatcg aacgcgtcat tcaagtgctc tgccgacgga cgaaaaacaa tccggtcttg
     2941 atcggtgaag cgggagtcgg caagacggca atcgccgagg ggctggcgca ggaaatcgca
     3001 aacggtaacg tgcccgaatt gctgcacgac aaacgcgtga tcacgctgga tctggcgttg
     3061 atggtagccg gaaccaaata ccgcggccag ttcgaagaac gcatcaaagc agtgatggac
     3121 gagatccgcc gttccaagaa cgtgatcctt ttcatcgacg agctgcacac gattgtgggt
     3181 gcgggttcgg ccgagggcgc gatggacgcg tcgaacatca tcaaacctgc gctcagccgt
     3241 ggcgaattgc aatgcgtcgg cgcgacgaca atgaacgagt atcgcaaata catcgagaaa
     3301 gatgcggcgc tcgagcgccg ttttcagacc ataaaggtgg atgcacccac cgtcgatgag
     3361 gcgatccaga tcctgaaggg tttgcgccca aaatatgaag cgcatcacaa ggccaaactg
     3421 actgacgaag cgcttgagac cgccgttaga ttttccgacc gttatatcac cggacgcttt
     3481 cttccagata aagcgatcga tgttatggat gaggccggcg cgcgcgcgcg catcaacgcc
     3541 atgacgcggc ctcctgacgt caaggaaatc gagaaggaaa tcgaagagat ccggcttgaa
     3601 aaggaagccg cgatcaaggc gcaggatttt gaaaaagccg ccgcgctccg ggacaaggaa
     3661 aaacaaacta aagagaagct ggatacaatt ctggccaggt ggcgcgagga acgcgaagaa
     3721 agagaagtgg tggtgacggc agacgacatg atgcacatca tctccaaggt caccggcgtt
     3781 ccgcttcagc gcatggaaca ggaagagacg caaaagctgc tcatgatgga atccgagatg
     3841 aagcagcggg tgattggcca ggacgaagcc gtcactgcca tcagcaaggc tttgcgtcgc
     3901 tcacgtgccg atttgaaaga tccaaggcgc ccgatcggtt cctttgtctt tctcggaccc
     3961 accggtgtcg gcaaaaccta tctcgctcgc actctggctg aattcatgtt cggcgacgcc
     4021 gacgcgctta tccagatcga catgtcggag tacatggaaa aattcaccgc ctcgcgattg
     4081 attggttcgc cgccgggcta cgtcgggtat gaggaaggcg gccaactttc tgaggcagtg
     4141 cggcggcggc catattccgt tgtccttttc gacgaaatcg aaaaggcgca cccggatgtg
     4201 atgcatttgc ttctacagat tctcgaagac ggaaaaatca cggactcgtt aggccggaag
     4261 atcgatttcc ggaacacaat catcatcatg acctcgaatg tcggggcgga tttgctcaag
     4321 aaacaaacgg tgatgggttt cggcgcgccg ctcgaaggac atgactacgt ttcgatgcgc
     4381 gacaagattc tcgatgaaac caagcgcgtc tttaagcccg agttcctcaa tcgtctcgac
     4441 gagatcatcg tcttccattc gctgggcaaa ccggagttgc tgcgcatcgt cgatcttgaa
     4501 gtcgacaagg tgctcacgcg gatcaaaggg aaagacgttc aaattgaact gagccagagc
     4561 gcgaaggaat tcctcatcga gaaaggatac gatccgcaat atggtgcacg gccgatgcga
     4621 cgcggagttg agcgttatct cgaggatccg ctcgccgagg agctgctcag aggccacgtc
     4681 aaggcgggag accgggttga ggttgtggcc gccgaaggaa gactcacatt ccacgtcgcc
     4741 gaatcgcaac cgcagagtag cacggctgcg cccactactg gctagcattt cgactcggcc
     4801 atcacgcgcg cggatgcgcg tcagcgtagg ctttcttcaa ccgttccacg gtcacatgcg
     4861 tatagatttg ggtcgtggaa agactggcat ggccaagcag cgcctggacg ctgcgcaggt
     4921 cggcgccgcg atcgagcata tgggtcgcga aactgtgccg cagtttgtgc gggctaatcg
     4981 agattggaat cgatgtgtgc cgcagataac gtttcagaac aagccagatt gagcgcgtag
     5041 acattcgcct gcgcgatttg tttatgaaaa gcggtcctga atgcacattg gccgctgcac
     5101 gataactcga gatcgcttca agcgccggca aaccggccgg acaaacgcgt tcctttctgc
     5161 ctttgcctaa cacgcgcacc gactccgtgt agagatccac attccccaca tccagagcgg
     5221 ccagttcgga cagccgcaag ccgctactgt aaaagagttc catgatcgcc acatcacgta
     5281 acggcatcca gagtggggcg gcccggtttt tcggcacacg tgcgggcgca gcaagaagtt
     5341 cctcgacctg ctggcgcgtg agcacgagcg gaagcttctt gtcgagcttt ggcaattgca
     5401 cttcacgcac aggattactt cgcagatctt ttcgcgccgc gagaaactga tagaagctgc
     5461 ggaacgcgga gaattgcaat cgcacataag accgtgcctg cccacgcttc ataagtgcaa
     5521 agaggtaatc gcgaaaatcg tccgccgtgc attttttcca tgacgttttg ttctccgctc
     5581 gaaaaccagt cagcgcctgg cgataggctt tgagcgtgcg tgacgaagca ttccgctcca
     5641 cggccagata acgcagaaat tcctcggcaa gaggatccgt tttgttcaag gcgctcgctg
     5701 cttttgtcat gtcgagcgaa gtcgagacat ctcttactgt caaatgacgc ggtgtgccag
     5761 ggataacagt gagagatttc ctcgacttcg ctcggaatga caacggcaat gtattattca
     5821 ggtttttcaa ccgcgtaacg cagttccggt ggcgtcccgg tgccgcggaa ttgacttttg
     5881 aaaatcggcg cggggtttgt tgagattagt tcgtaaccct tcaatacgaa ctctggataa
     5941 acgctgttac tcgctggctc ataaactttg tcgccgctaa actcgccgta gagtttgtat
     6001 tcgtaattat tgtcattccc aaactcgagc tgttcgcggt ccggtgcgag cttttgtttt
     6061 tcgttcagca tcaccaattg cgccgtgctc caaggttgtc cgggacgccg gacgtagccc
     6121 cagaacttgt aatcaggttt gtaataacgc cgtccgatat agtaatcccc aggctgttcg
     6181 gtggtgatgc gctgcgccat ctcgatcctt gcctgctgga tcccttgcgg aacggtttgg
     6241 caaccgttca gaaacagcgc gccaagcaat ccggcagctg ccggactcgc aagcagaaga
     6301 tgaacagcgc gcctcattct cgggtcgaca tcttttatcg cagctttacg ccgcacgcaa
     6361 attatcggag aatcaatggc gatcaggctt ggcccgggac tggcaatata aaaggctctt
     6421 gcagtgccgc cattcaccaa tcactaatca gcgatcacct gcaaatatga tggtccgctt
     6481 ctttaacgcg ctgggcacgc cgctgctcgc gtttgttcaa tatttggggg aagtcgtgct
     6541 tctcgctgcc gacacctttc gttgcatttt tactcacagg ctccggtgga aattatttct
     6601 caatcaagtc gtcgagatcg gcttgctttc gcagctcgtc gtcgtaatca ccggcgcatt
     6661 caccggtgcg gtgttctcgg cgcaaacatt tttccaattt aacaagctgg gaatgggctc
     6721 agcggtcggc gccgtggttt cggtgtccct ctgccgcgag ctggctccgg tcttgacggc
     6781 gttgatggtg gccggacggg tcggcgcggc gatgtcggcc gagatcggca ccatgaaagt
     6841 cacggagcag atcgatgcgt tgcgggcgct cgcggtttat ccggttgatt atctggtcgt
     6901 gccgcgcgcg cttgccatga tggtatcgat gccgctgctg gtggcggagt gtatcgccgt
     6961 cggcatcatc gccggctatt tcgtcgcgat tttcatgctc gacattaacg gcacctacta
     7021 catggcgaac atggagcgct ggacgcgcag tcgcgatatc atcatgggcc tgtcaaaagc
     7081 gttctgtttc ggaatcctca tcgtgttcat cagttgccac aagggattga ccgcacgcga
     7141 aggcgcggtt ggcgttggcc gggcgacgac tgaggcggtc gtgaactcct cgctcgcggt
     7201 gctgatttgc aattttttcc tcaccatgtt gttcaacatt atttacccgg cgggatatca
     7261 atgagtgatg agttgaacct tgaacgcttg ctgcgccgta gccttgcgaa ggcgggttga
     7321 gcgttgaatg tttcggcgat gattgaagcg ccgccatctc cagaaccatc gattacgcct
     7381 cgtttggttt cgagcgactc ttcgccgatg atcgcggtac gttcattgac gaagaaaatc
     7441 gaccggcagg aaatcctgcg tggcgtcgat cttgaggtcg caaggggtga gacactcgcc
     7501 atcattggtc ggagcggcgg cggcaaaagc gttttgctta aacatctcgt gggactgatg
     7561 cgaccgaatg ctggagaaat ttggatcgaa ggacaaaaca tcaccaaaat gaatgagcgc
     7621 cagctcgggg cgatccggca gaaggttggc attctttttc aaggcggcgc gctttttgat
     7681 tcgatgaccg ttgaagaaaa catcgccttc ccattgcgcg aagctggtga acgcgacccg
     7741 aaagtcttgc gcgcgaaagt aaatgagatg ctcgaagtga tggagatgga aggcgaacaa
     7801 tcgaagatgc ccgtgagctt aagcggcgga atgaagaaac gcgtcggtct cgcgcgttcg
     7861 attattcgcc ggccatcctg cgtattgtac gatgagccga catccggact tgatccggtg
     7921 gtcgcggaca gcatcaatcg attgattcgc cggctacagg agcgctttgg agtgaccagc
     7981 atcgtcgtaa ctcacgatat gaaaaatgcg gttcacgtgg ccgaccgaat cgcatacttg
     8041 catgagggcc gaatttattt tcatggcact tcggctgaac tccagaaatc aagcgacccc
     8101 ctgatccaag atttcctcct cggacgctcc gatgggcaat gagagaacca ggttgagagt
     8161 taaaggttca aagataagag ttggagactg atgaatcggc acgagcgagg tttggaattt
     8221 aaagtcggca ttttcgtttt cgtgggattg gcgatgctag gcgcgctcgt cgtgcaattc
     8281 ggcaggctgg gggaggggtt caaaacctat tacgccctta ccgttcgctt caacgatgca
     8341 agcggcctgt tgaaaggctc cgatgtcttg ctcgccggcg cgagaattgg gaaggtcgca
     8401 gacggtccgc ggctggttga aggcggtggc atagctgtgc cactgaaaat ttatgattac
     8461 attaagatcc ctgagggggc caaattcacg gttggaagca caggtttgct cggcgatcgg
     8521 ttcgtaaatg taactatgcc gcctgggcag ccgaaggcgt atcttccgcc gaacgcttct
     8581 atcagcggtg cgcgcgagac tggcattgac gatctcaccc gcgagggcgg ggcgctggtg
     8641 ggcgatctcc gcgggaccgt gcaaaagatc gacacgactg tgaatcgctt gaaccaggac
     8701 acgctttcct ccgcgaacat ggaaaatctg aaatcgagca tggagcattt aaatcaggcg
     8761 accggtgcgc tcgcggaatc ctcaaaaaaa ttggatggcg tgatcgagca ggcagattca
     8821 accatgactt cggctaaaga agcagccgat aatctgcaaa atgcgattgc cgacgcgcgc
     8881 aaagtgttga gcagcgcaac aaaagggaaa ggtttggtag cggccctttt aaatgatcag
     8941 cagttagcga acgatctgca cgcgcttatc actaatttac ggacgcacgg cgttttattt
     9001 tatcgggatt cagccgcgaa aatcgaggcg aaggcgcagg agcaaaccaa accggcgcgt
     9061 cggactcggg gtcgctaagg ttgggactgt tttcctgagc gctgaagcgc ttgggtgttt
     9121 tcaggtttca aggtcttgcg ttaagcctga atcgattgcc gtcggatgag tgggctggta
     9181 aagaccgatc tcaccaccgc tcggaagctt gactgaggtt gtgattcccc attcggctgt
     9241 ttctattgcc gtgcagctta cattcttgga tttcaacttc ttgatcacct catccagatt
     9301 atcgcacatg aggtaaagga ccgagccaag cagtgaatgc tctgcgtgag tttgcacgaa
     9361 attatccatg gaaggatgta tgccgagttc tgccggaggc agcttgaaaa tcaaccatcc
     9421 gccgcctgca tcgacggagc gaaagttcag gatatcgcgg aagaacgctc gatctgcttc
     9481 tggatcttta ctgtacaata gaacgtgtgt gccggtaatc atgcagagag tatatgccga
     9541 cagccatcag tcggctagcg tcgtatcaat gccagcaacc ggcgtcgcgc gtcttcttcg
     9601 cctaatccgt cgatgcgaat tagtttgtcc cgcgattttt gacagcgcac gagcgcaatg
     9661 ttccgttcag gaatccctaa ttcttcggcc agaaactgcg cagggcagca ttcgcttttc
     9721 cttcgactgc cggagcacgc agcgtgattt tgattgccgc tccatgcgcg ccgatcacct
     9781 gattcgcttt cgcattggga acaatgcgaa cagggagagt cgccatttgt gcaaactgta
     9841 gcggcgctaa tagccggtcg ctacagcccg actgccgcgc gcacgcgctc catgacttta
     9901 tccgcaactt cgtttgcgcg ttttgcgccg cgcgcaagca cattgtcgat gtacgatttg
     9961 tcggcgagga tttcttcccg gcgtttgcgc atcggcgcga aataatccca aagcttttcg
    10021 aagagttgtt ttttgaaatc gccgtagcca ttgccgcctt tttgaaaccg ttcgcgcatc
    10081 tcggcaattt cgttttttga agcgacgagc gagtagaggc ggaagattat tgatctggag
    10141 gggtcctttg gcgcttcgac cggcgtggaa tcggtcacga tgctcatgat ccgtttccgg
    10201 gtttcttttt cgttaccgaa aatatcgatg ttattgccgt agctcttgct catcttttgg
    10261 ccgtcaatgc ccgggacgac ttcagtcgcc gcctgaatgc gcggctcggg gagcttgaaa
    10321 atccgcccgt ccggctcgga cccaaacctt tcgttcatct tgacggccag atcgcgcgta
    10381 atttcaatgt gctgtttttg atctttgccg accggcacga tgtcgctgtc gtaaatcaga
    10441 atatcggccg ccatcagcac cggataattg aagagcccga cagtcgcggc cataccccgg
    10501 gc
//
LOCUS       DASQUQ010000174         8640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_577898, whole genome shotgun sequence.
ACCESSION   DASQUQ010000174 DASQUQ010000000
VERSION     DASQUQ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577898"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..916
                     /gene="proC"
                     /locus_tag="VE486_04990"
     CDS             68..916
                     /gene="proC"
                     /locus_tag="VE486_04990"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZA38474.1"
                     /translation="MSLKLSDKKLAVLGAGKLGGILLRAYLKQGLFEAKRVTATVKHG
                     EKTAARAKQLGVAVTSDNRKAVKDAEVILLAVKPQAVAEVLDEIAPEIRAKRLLVSVA
                     ASVPTSYLEQQLAAASGGQSNKVAVVRAMPNTPAAVGCGMTAICRGAHAGPADVEIAQ
                     TMFDAVGRTVVVDEKNMDAVTGLSASGPAFAYIILESLAEAGVKVGLPRDVATLLAAQ
                     TMKGSASVVLETGDHPALLKDAVTTPAGCTIDGILELEEGKLRVTLIKAVVKATSRAG
                     ELLFEK"
     gene            921..1055
                     /locus_tag="VE486_04995"
     CDS             921..1055
                     /locus_tag="VE486_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38475.1"
                     /translation="MPRRKNRDVSVSGRIDTLTNEHYAEVFGFPALGRAAYGGLKLRF
                     "
     gene            1221..2423
                     /gene="rmuC"
                     /locus_tag="VE486_05000"
     CDS             1221..2423
                     /gene="rmuC"
                     /locus_tag="VE486_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZA38476.1"
                     /translation="MTPWLYMIAGVIAGGLLAAIIFLLGRRGKDDAAAVLGKIELLER
                     AQEREERMLREEMALSREEGANAAKAQREELGKSLESVRSIVDLRLKELQEHNASQID
                     KMRATVDEKLQGTLEKRLGEAFRTVSDRLEQVHQGLGAMQQLASDVGGLQRVLTNVKT
                     RGGWSEWQLGVLLEEMLTPDQFAQNMKTRDDTDERVEFAIKLPGDENGAPVWLPIDAK
                     FPMEHYERLTTAQEKGDPTAVEGAMKTLETQLKRCAKDICEKYINPPKTTDFALLFLP
                     SEGLYAEAIRRVGLVQNVQRDCRVTFVGPTTLAALLNSLQMGFRTLAIQKRSSEVWNL
                     LAAVKTEFGKFGEALSGVKEKLDQAARKIEDVDVRSRAITRKLRDVEELPSNPQPMLK
                     DWSSGVEE"
     gene            2431..3129
                     /locus_tag="VE486_05005"
     CDS             2431..3129
                     /locus_tag="VE486_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZA38477.1"
                     /translation="MENKTALVTGANKGIGYEVARQLAGKGFHVFVGARKRDAGRKAA
                     NEIAKAGGRATFLEIDVADNASVSAAAHEFSEIADHLDVLVNNAGIIVDGDNEILEVS
                     DDLFRKTLETNTLGPLRVTRAFAPLLAKSKTPRVINVSSGGGQLTGGADGWSPAYCIS
                     KTALNGVTSQLATALPKFAVNSVCPGWVRTDMGGQGATRSVEEGADTIVWLASDAPQK
                     LTGKFLRDRKEIPW"
     gene            complement(3286..5034)
                     /locus_tag="VE486_05010"
     CDS             complement(3286..5034)
                     /locus_tag="VE486_05010"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZA38478.1"
                     /translation="MFILLMLTLCFASSFAAQTADADYEAVAEEYLKGYLAARPLRGT
                     ALGLHEYDGKITDYSRLALDAELSRLRRFDDRLNKFDPAKLSQRQSIDLRILQAAVKK
                     ELFQMLDMSVFERNPMIYARAADLNVYVQRNFAPLEDRVHSLIAIESEVPNILIAART
                     NLNEVLPKPYVELAIQIARGSADFLKKDLPAAIGEIKNEQLRAAFQFSNRKAASALSD
                     YAAWLEREKLPKASLDFALGDEKFRRLLAQTELVDLPPQKILEIGMEQLKAEQEAFAN
                     AAKIIDPNKSPIEVFRQIQSEHPAPENLIPDVGKGLDKIRKYLTSRKLVTIPSDVRPK
                     VKETPQYRRATSFASMDTPGPFEKRATEAYYYVTPTENDWPEKQKEEWLTAFNYYTSD
                     IVSIHEAYPGHYVQFLRLNASSASKVEKIFGSYAFVEGWAHYCEKLMIDEGYGTATSS
                     TPSEEDVKRAAKYRMAQADEALLRLCRLCVSIKMHTQKMSIDEATKFFQDNCYYEEKP
                     ARSEAMRATFDPLYLSYTLGKLQILKLRDDYKAQQGDEFSLQKFHNELLNHGMPPIRL
                     LREIMLKDQSKWDQVL"
     gene            5140..5268
                     /locus_tag="VE486_05015"
     CDS             5140..5268
                     /locus_tag="VE486_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38479.1"
                     /translation="MAKAVRGKLPRTTGQRPVLPREKPAPAVTQISQTITHVASLH"
     gene            5364..6644
                     /locus_tag="VE486_05020"
     CDS             5364..6644
                     /locus_tag="VE486_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZA38480.1"
                     /translation="MLSLACLLFGVLSGLRAQEAQQGKAEEDNGYAQISIFAKAIELI
                     RQDYVDGSKTTYHDLITAAMKGMLSSLDPHSQFMDPDDFRDMQEDTRSRFNGLGIEVS
                     MKNGLPTVITAMEDTPAARAGILSGDQILKINGASTERMDLQDAINILRGPAGRRVTL
                     TLLRPSTKEIKEYTLDRAEIKVRSVKGARLLDAELTGPFRIGYIRLIQFNEPTAEELS
                     KALDELQKQGMQALILDLRNNPGGLLTSAVDVCAQFLPPKSKVVSTQGRMASQQHEYS
                     TSGAKKDRPNFPMALLINEGSASGAEIVAGALKDLHRAILVGETTFGKGSVQNVIQLP
                     DGSAVRFTTAKYYTPGQQVIQGNGVVPNIRVAMTAEQERALIALRNAENVKPEDEKSI
                     IKTKDPQMLRAIDALKGLMIYAQQSAPKAEAVKK"
     gene            6708..7817
                     /gene="tsaD"
                     /locus_tag="VE486_05025"
     CDS             6708..7817
                     /gene="tsaD"
                     /locus_tag="VE486_05025"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZA38481.1"
                     /translation="MTVLAIETSCDETAVAILRGPNGRFGSELLASEVASQVAEHEKY
                     GGIVPEIASRNHLVHAPRLLERATRDAKIDTSEVDAFAATSGPGLASSLMIGASIAKG
                     LAIGFGKPYLAINHLEGHLLSPFFGDGEVKPNVSLIVSGGHTMLVWVRGLADYQLIGC
                     TVDDAAGEAFDKVAKMLGLGYPGGPEIEKLAPDGDPNRFDLPRSMLGSENFSFSGLKT
                     AVRYLLPKIGVIPSRADGEGPRGRTLDHEGEMHDQLSCERSFASAQDAKLLSDLCASF
                     QQAIIDVLVTKTIAAAQKYDVDLVTVSGGVSCNQQLRKQLRDACARQGFEFKPAEPWL
                     CTDNAAMIAFAALLRLQAGFESKLTEEIDPNLALA"
     gene            complement(7968..>8640)
                     /locus_tag="VE486_05030"
     CDS             complement(7968..>8640)
                     /locus_tag="VE486_05030"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZA38482.1"
                     /translation="ALECIHTYSLVHDDLPSMDDDDFRRGRPTCHKVFGDGIAVLAGD
                     ALLTIAFEIVSTAKPAPRYDMSILLREIAVAAGSQKLIAGQVADLEAEGRNVKREQLR
                     YIHENKTAAMLTSSVRVGAMSANADAKKLRAITNFGRGLGLAFQVIDDILDVTQTSEI
                     LGKSAGKDVAAKKATYPAVIGLEKSRAEARRLTRQAHNALSVFTSGQAEPLHCLANYL
                     LEREY"
BASE COUNT         1922 a         2276 c         2609 g         1833 t
ORIGIN      
        1 gcgtgccaag ccgtagcttt atgcgaaggt ctggaggaat ctctcgttgt tcggcaaagc
       61 attccccatg tccctcaaac tttctgacaa gaaactggcc gtgctcggcg ccggcaaact
      121 gggtggaatc ctactgcgtg cctacctgaa gcaaggactg ttcgaagcca agcgcgttac
      181 cgctactgtg aagcatgggg aaaaaacagc cgcgcgcgca aaacagctgg gcgtggccgt
      241 aaccagcgac aatcgcaaag cggtgaagga tgccgaggtc attctgctgg cggtcaagcc
      301 ccaagccgtg gcggaggtgt tggacgagat cgctccggag attcgcgcca agaggttgct
      361 tgtctccgtt gcggcttcgg tgccgacgag ctatctggag cagcagctcg ctgcggcgag
      421 tggcggtcaa tctaacaaag tggcggtcgt tcgagcgatg ccaaacacac ctgccgcagt
      481 tggctgcggt atgacggcca tttgccgagg tgctcatgcg ggaccggccg atgtcgaaat
      541 tgctcaaacc atgtttgacg ctgtgggccg taccgttgtt gtcgatgaaa agaacatgga
      601 cgcggtgacg ggtctttcgg cgagcgggcc tgcgttcgca tacattattc tggaatcgct
      661 tgccgaagcc ggggtgaaag tcggtttgcc gcgcgatgta gccacgcttc tagcggcgca
      721 gaccatgaag ggttcggcga gtgttgtttt ggaaaccggc gaccatcccg cgctcctaaa
      781 ggacgctgtc accaccccgg ccggttgcac cattgacggc atcctcgaac tggaagaagg
      841 gaaactccgc gttacgctga tcaaggcagt cgtcaaagcg accagccgcg ccggcgaatt
      901 gctatttgaa aagtagtcgc gtgccgcgac ggaaaaatcg cgacgtgtcg gtctccggcc
      961 ggatcgacac ccttacgaat gaacactacg cggaggtttt tggctttccc gcgctcggcc
     1021 gcgccgcgta tggcggcttg aagcttcgct tctaatcatc attgtcatcc cgagccgcga
     1081 agacggagag ggacctcatt tttgtcttct ggcacacgtg cgttacgaag catgatcgac
     1141 gtgcttgtgt gaggtccctc gctccgctcg ggatgacagg cacatttgaa cgcgcgctct
     1201 aattcgagta ttcacgcttt gtgactccat ggctttatat gatcgccggc gtgatcgctg
     1261 gcggcttgct tgcggcaatc attttcttgc tgggacggcg cggcaaagac gatgccgccg
     1321 ctgttttggg aaaaatcgag cttctggagc gcgcccaaga gcgcgaggag cggatgcttc
     1381 gcgaagaaat ggcactcagc cgcgaagagg gcgccaacgc cgcgaaagcg caacgcgaag
     1441 agttgggcaa gtccctcgag agcgtgcgtt cgattgtcga tcttcgcttg aaagagttgc
     1501 aggaacataa cgccagccag atcgacaaaa tgcgcgcgac cgttgacgag aaattgcaag
     1561 gcacactgga aaaacgcctg ggcgaagcgt tcagaacggt gagcgaccgg cttgagcagg
     1621 tgcaccaggg attgggcgcg atgcagcagt tggcgagtga cgtcggtggg ttgcagcgcg
     1681 tgctcaccaa cgtgaaaacc cggggcggct ggagcgaatg gcagctcggc gtcttgctgg
     1741 aagaaatgct gacgccggac cagttcgccc aaaacatgaa gacgcgcgac gacaccgatg
     1801 agcgcgtcga gtttgcgatc aagttgcccg gtgacgagaa tggcgcgccg gtgtggctgc
     1861 cgattgatgc gaaatttccg atggaacatt acgagcgttt gacgacggcg caggaaaaag
     1921 gcgacccaac agcggtggag ggagcgatga aaactttgga gacgcagttg aagcggtgcg
     1981 caaaagacat ttgcgagaag tacatcaacc cgccaaagac gacggatttt gcgcttctat
     2041 ttttaccaag cgaaggactt tacgcggaag cgattcgtcg cgtcggcttg gtccaaaacg
     2101 tgcagcgcga ttgtcgcgtc acttttgtag gaccgaccac gctggcagcg ctgctcaaca
     2161 gtttgcaaat gggctttcgt acgctcgcta ttcaaaagcg ctccagcgaa gtctggaatt
     2221 tgctcgcagc ggtaaaaacc gagttcggga aattcggcga ggcgttatcg ggagtgaagg
     2281 agaaactcga tcaagccgcg cgcaagattg aagatgtcga tgttcgctcg cgggcgatca
     2341 cgaggaaact gcgggatgta gaagaattgc cgtcgaatcc gcagccgatg ctgaaagatt
     2401 ggagtagtgg agtagaggag tagtggaggg atggagaaca agactgcatt agtgaccgga
     2461 gcgaacaagg gcatcggcta cgaggtcgcg cgacagttgg ccgggaaagg atttcacgtg
     2521 ttcgtcgggg cgcggaagag agatgccggc cgcaaagcgg ccaatgagat cgcgaaggcg
     2581 ggtggaaggg cgacgttcct tgaaattgac gtcgcggata atgccagcgt cagcgcggcc
     2641 gcgcatgagt tctcggagat tgcggatcat ctcgatgtgc tcgtgaataa tgcggggatc
     2701 attgtcgatg gtgacaatga gatcctggaa gtgagcgatg acttgtttcg gaaaacgctg
     2761 gagacgaaca cgctcgggcc tttgcgggtg acgcgcgcat ttgcaccctt actcgcgaaa
     2821 agcaaaacgc ccagggtgat caatgtctcc agtggtggcg gacagttgac cggcggcgca
     2881 gatggctggt cgccggctta ttgcatttcg aagaccgcgc tcaatggcgt cacgtcgcag
     2941 ctcgccactg cgttaccgaa gtttgcggtg aactcggtct gcccgggttg ggtgcggacc
     3001 gacatgggag gacagggcgc gacccgctcg gtggaagaag gcgcggatac gatcgtctgg
     3061 ctcgccagcg atgcgccgca gaagctcacc gggaaatttc tgcgcgatcg gaaggaaatt
     3121 ccgtggtgag ctgcgtttct gtagccgctt cgctgtgcga agcgcggcgt tgaccggcaa
     3181 aacagactac agcggcgtcg cccacagggc gacggctaca gccgatgagc atcgagcacg
     3241 atcacgagca tgagcacgac taagaatagg aggaagagga agaaattaca gtacctggtc
     3301 ccatttgctc tggtccttaa gcatgatctc gcggaggaga cgaatgggcg gcatgccgtg
     3361 attgagcagc tcgttgtgga acttttgcaa tgaaaattcg tcgccttgct gggccttgta
     3421 atcgtcgcgc aatttcagga tctgcaattt gccgagcgtg taactaagat agaggggatc
     3481 gaaggtggct cgcatcgcct cgctccgagc cggcttttct tcgtagtaac aattatcctg
     3541 gaaaaacttc gttgcctcgt cgatcgacat tttctgcgtg tgcattttga tcgacacgca
     3601 caagcggcac aagcggagga gcgcttcgtc ggcctgtgcc atgcggtatt tggccgcgcg
     3661 tttcacgtcc tcctcgctgg gagtcgagct ggtagcggtg ccgtaacctt cgtcgatcat
     3721 cagcttttcg cagtaatgcg cccacccttc aacgaaggcg tagctgccga aaattttctc
     3781 gaccttgctg gccgacgatg cgttcaggcg cagaaactgc acataatggc caggatacgc
     3841 ttcgtgaatc gagacgatgt cggaagtata ataattgaaa gcggtgagcc attcttcctt
     3901 ttgtttctcc ggccaatcgt tctccgtagg cgtcacatag tagtaggctt cagtcgcgcg
     3961 tttctcgaag gggccgggag tgtccattga ggcaaaggag gtagcgcggc gatattgcgg
     4021 ggtttctttc actttcggtc gaacgtccga gggaatggtc accaatttgc ggctcgtgag
     4081 gtatttgcga atcttgtcca ggcctttgcc cacatcaggg atgagatttt cgggcgccgg
     4141 atgttcgctc tggatttgcc tgaagacctc aatcggcgat ttgtttgggt cgattatctt
     4201 tgcggcgttg gcgaacgctt cctgttccgc ttttaattgc tccatgccga tctccagaat
     4261 tttttgcggc ggaagatcaa caagttcggt ttgggccaac aggcgccgga acttttcgtc
     4321 cccgagagcg aaatcgagcg acgcctttgg aagtttctct cgttcaagcc acgccgcata
     4381 atcggagagg gcgctggctg ctttgcgatt ggagaactga aacgcagcac gaagctgttc
     4441 gttttttatt tcgccaatcg cggcagggag gtctttcttt aggaaatcag cggagcctct
     4501 ggcgatctga atggcaagtt ccacatacgg cttgggaagg acttcattca ggttcgttct
     4561 ggccgcgatc agaatatttg ggacctcgga ttcgatcgcg atgagactgt gaacgcgatc
     4621 ttcgaggggc gcaaagttcc tttgcacata aacattcagg tcagcggcgc gcgcgtaaat
     4681 catgggattt cgctcgaaga ccgacatgtc cagcatttga aagagttcct tcttaacggc
     4741 cgcttgcaga attcgcagat cgattgactg gcgttgactc aatttggctg ggtcgaattt
     4801 gttcaatcga tcgtcgaacc gccgtagccg ggaaagctcg gcatccagcg cgaggcggct
     4861 gtaatcggtg attttgccgt catattcgtg aagcccgagg gcagttcccc gaagcgggcg
     4921 agcggccaag taacccttaa gatattcctc ggctaccgct tcgtaatcgg catcggcagt
     4981 ttgcgcggcg aagctgctgg cgaaacagag cgtgagcatc aagaggatga acacgagagc
     5041 taacgcgcgc tcattaacgc tgggctttct ggggagcaca ggccgccggc ctgtagcatt
     5101 cggccgctgg ccgaatgtcc ggcatcgaaa acgcgcgaga tggcaaaagc tgttcgcggc
     5161 aagctgccgc gaactacagg ccagcggcct gttctcccca gagagaagcc ggcgccggcg
     5221 gttacccaga tcagccagac gattacccat gtcgcgagtc tccattaaga caacggcgtg
     5281 ctttgggcgt tgccaaaaat cccaaatgag cgatggtgct gtccctttca atattggttc
     5341 gaaatatgag acgccgggtt gtcatgcttt cgttagcttg cctgttgttc ggcgttctct
     5401 ccggcctccg cgcacaagag gcgcagcaag gaaaggccga agaggataac ggttacgcgc
     5461 aaatttccat tttcgcaaaa gccattgaat tgattcgaca ggattacgtg gacggcagca
     5521 agacgaccta ccacgacctc atcaccgcgg cgatgaaagg catgctttcg tcgcttgatc
     5581 cacatagcca gttcatggat ccggatgatt tccgcgacat gcaggaggac acgcgcagcc
     5641 gtttcaacgg tctcggcata gaggtctcga tgaaaaacgg tttgcctaca gttattactg
     5701 cgatggagga cacgcctgcg gccagagcgg gcattctgtc gggcgatcag atcttgaaaa
     5761 taaacggtgc ttcaaccgaa agaatggatc tccaggatgc catcaatatt ttacgcggac
     5821 cagccgggcg cagagtgacg ctcaccctgc ttcgcccctc gaccaaggaa atcaaagagt
     5881 acaccctgga tcgcgcagag atcaaagtgc gcagcgtcaa aggagcgaga ttgctggatg
     5941 cggagctgac tggcccgttc aggatcggct atatccgact gattcagttc aatgagccga
     6001 cggcagagga attatcgaag gcgctcgacg agctgcagaa gcagggaatg caggcgctta
     6061 ttctcgatct gcgaaacaat ccgggcggat tattaactag cgcggtggac gtgtgcgctc
     6121 aatttttgcc gcccaaatcg aaggtagtct caacgcaggg cagaatggct tcccaacagc
     6181 atgaatactc gacttccgga gcaaaaaaag atcgcccgaa ttttccaatg gccctgttga
     6241 ttaacgaagg cagcgcaagt ggcgccgaga tcgttgccgg cgcgctcaag gatctgcacc
     6301 gtgccatcct tgtcggtgaa accacttttg gaaaaggttc cgtgcagaac gtcatacaat
     6361 tgccggacgg ctctgcggtg cgttttacta cggctaaata ctatacgccg ggccagcagg
     6421 tcattcaggg aaatggtgtg gttccaaata ttcgcgttgc catgaccgcc gagcaggagc
     6481 gcgcgctcat cgccctgcgc aacgcggaga atgtgaagcc cgaggacgaa aaaagcatca
     6541 tcaagacgaa agatccacag atgctgcgcg caattgacgc gctgaagggg ctcatgattt
     6601 acgcgcagca gagcgcaccg aaagccgaag cggtaaagaa ataactgtag ggcgaccgcg
     6661 tcggttgccc ggcttggcag gcggagcgcc tgctctacaa ttgtcctgtg acggtcctgg
     6721 caatcgaaac atcttgtgac gaaaccgcgg tcgcgatttt gcgcggaccc aacggccgct
     6781 tcggttcgga gttgctggcc agtgaagtcg cgtcacaagt cgccgagcat gaaaaatatg
     6841 gcggaatagt gcccgagatc gcttcgcgca atcatttggt gcacgctccg cgccttctcg
     6901 aacgggcgac acgcgacgca aagatcgaca cgtcggaggt cgatgcattt gcggcaacca
     6961 gcggacccgg cctggcaagc tcgctcatga tcggcgcctc cattgccaag ggactggcaa
     7021 tcggcttcgg caagccgtat ctcgcaatca atcacctcga aggccacctg ctttcgcctt
     7081 ttttcggcga cggcgaggtg aagcccaacg tttcactgat cgtgagtggc gggcacacga
     7141 tgttggtgtg ggtgagaggt cttgctgatt accagttgat cggatgcacg gtcgatgatg
     7201 ctgccggcga ggcgttcgat aaagtcgcca agatgctcgg gctcggttac cccggcggcc
     7261 cggaaattga aaagctcgcc cccgacggtg atccaaaccg ttttgatctg ccgcgcagca
     7321 tgctcggctc ggagaatttc agtttcagcg gactaaaaac cgctgtgcga tatctcttgc
     7381 ccaaaatcgg tgtcatcccg agccgcgcag acggcgaggg acctcgcggt cgaaccttgg
     7441 accacgaagg ggaaatgcat gatcaattat cctgtgagag gtctttcgct tccgctcagg
     7501 atgccaagct gctgagcgac ctctgcgcat cgtttcagca agcgattatc gacgtgctgg
     7561 tgaccaagac gatcgccgcg gcgcagaagt acgatgtcga ccttgtgaca gtcagcggcg
     7621 gcgtcagctg taatcagcaa ttgcgaaagc aattgcgcga tgcatgcgcg cgccaaggat
     7681 ttgaattcaa acccgccgag ccctggttgt gcaccgataa tgcagcgatg atcgcatttg
     7741 ccgcgttgtt gcgattgcaa gccgggtttg aatcgaagct gacagaggag atcgatccaa
     7801 atctggcgct ggcatgaatt tcgcgctcat ctcattctgg ggagcgtagc ctgtagtcgt
     7861 cggcagcttg ccgacgacac ttcaaaagcg taatccgatg cgaggcgcgt gaagactttt
     7921 cggcaagctg ccgaaaagaa caggctggca gcccatgctc cccggaatca atattcgcgt
     7981 tcgaggagat aatttgcgag acaatgcagc ggttccgcct ggccgctggt aaataccgac
     8041 aaggcattgt gcgcctggcg cgtgagccgc ctggcttcag cgcgtgattt ctccaagcca
     8101 atcacagcag ggtaggtcgc tttctttgcc gccacatctt tgccggcgct tttgcctaat
     8161 atctctgagg tttgcgttac atcgagaatg tcgtcgatga cttgaaatgc gagcccaagg
     8221 ccacgtccaa aattggtgat ggcccggagt ttttttgcgt ctgcgtttgc gctcatcgcc
     8281 ccgacccgaa ccgagcttgt caacatcgcc gccgtcttgt tttcgtggat atagcgcaat
     8341 tgctctcgtt tgacgttcct gccctcggct tcaagatccg caacctggcc ggcaatcaac
     8401 ttttgacttc ccgccgccac tgcgatctcg cgcaataaga tcgacatgtc atagcgtggc
     8461 gccggcttcg cggtcgaaac aatttcaaac gcgattgtca acagcgcgtc tccagcaagc
     8521 acggcgatgc cgtcgccgaa gactttgtga cacgtgggac gaccgcgccg aaaatcatca
     8581 tcatccatgc tcgggagatc atcatgcacc agcgagtaag tgtggataca ttccagcgcg
//
LOCUS       DASQUQ010000175         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_401274, whole genome shotgun sequence.
ACCESSION   DASQUQ010000175 DASQUQ010000000
VERSION     DASQUQ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401274"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..2330
                     /gene="ppk1"
                     /locus_tag="VE486_05035"
     CDS             42..2330
                     /gene="ppk1"
                     /locus_tag="VE486_05035"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HZA38483.1"
                     /translation="MPTAAAKVSVADEPSGGIELLRKDVPAANRFSDPRNFINRDLSW
                     LEFNRRVLEEAQDPTQPLIERVKFLSIFSSNLDEFFEIRVAGIKQQIESETSDVGPDG
                     LSPTETFNNIQRMVRELVATQYSLWNEELLPLLSKNGIRIPRMTELSAKRAAWAHKYF
                     QEEVFPMLTPLAVDASHPFPQLLNKSHNLLVRAKTQRGGERLHAIVQVPRVLSRLIAM
                     PRGKGDDEPWVYIYLASLIKQHIGELFPGLILDGVHAFRVTRNSDLYIDEEEAENLLR
                     TIEQELRRSSRGNAVRLEVEADCPKDFRDLLLGFFNLTEADVYKLDGPLSMTHLMPLV
                     TNDAFANLRDRPFQPARDPALPPHVNIFEVMRRQDVLLHHPYESFDPVVELIEEAAQD
                     PQVLAIKITLYRTSGDSPIIQALINAAVAGKQVTALVELRARFDEAANIQWARQLEEA
                     GAHVVYGVVGLKTHCKALLIVRRDSDRLRHYVHLGTGNYHPRTARIYTDFSLFTSEPR
                     LTEEVSVVFNTLTGLAGYPGLKKLLVAPFDLHKRLIGKIERERDHALAGTPGRIIAKL
                     NHLVDQETIEKLYEASCADVSIDLVVRGICSLRPKIPGLSENIRVFSIVGRFLEHSRI
                     YYFENAGQREVFLSSADWMPRNFFRRVELAFPLENPILRDEIVNEVLPSFLNDRVKAR
                     ELQPDGSYRRLKPEGKEPRAQAQWHFRKVSRERAKKMSGSKKKLRADKLIPITEADAK
                     SVAAAEHEPGSPISATTGNQSS"
     gene            2327..3016
                     /locus_tag="VE486_05040"
     CDS             2327..3016
                     /locus_tag="VE486_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZA38484.1"
                     /translation="MKKKILVIDIGGSNVKLMISRAKKLKFASGFRLTPREMVAQIKP
                     LVSDWKFDAISIGFPAPVRDGRILSEPKHLSKGWIGFNFEKAFGKPVRIINDAAMQAL
                     GSYHGRRMLFLGLGTGLGSALVWYNYVLPLELGDLPYRNGSIIEDYLGKPGLTRLGEK
                     TWRRDVEHALVQLKKSLIADYVVLGGGNAKKLDALPEGIERGHNRNAFLGGTRLWQID
                     SRTRRPKWQIL"
     gene            3129..3590
                     /gene="sixA"
                     /locus_tag="VE486_05045"
     CDS             3129..3590
                     /gene="sixA"
                     /locus_tag="VE486_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HZA38485.1"
                     /translation="MELYFLRHGEADWPDWKKSDDERPLTKRGKKEMHAVAAFLVRLK
                     VQPELILTSPLPRAAQTAEIAAEHLDVKCREEQRLAPGFGAAELEQIINKHSVESLMI
                     VGHEPDFTRTIAALTGASLKLSKAGVALVDLDVSSRRGRLLWLFPPKIAKR"
BASE COUNT          886 a         1027 c         1032 g          816 t
ORIGIN      
        1 gcgcgtggcc gttttcggtc acggatttaa ggaaaaagta aatgcctaca gctgctgcca
       61 aggttagtgt cgccgatgag ccgtccggcg ggatcgagct tctgcggaag gatgttcccg
      121 cggcaaatcg tttctccgat ccccgaaatt tcatcaaccg cgacctgagc tggctcgagt
      181 tcaatcgccg cgtcctcgag gaagcgcagg atcccaccca gccgctcatc gaacgcgtca
      241 agttcctgtc catttttagt tcaaacctgg atgaattttt tgagattcgc gtcgccggca
      301 tcaagcagca gatcgaaagc gagaccagcg atgttgggcc tgatggtttg agcccaaccg
      361 agacattcaa caacattcaa cgcatggtgc gcgaactggt cgcaacacaa tactcgctct
      421 ggaacgaaga actgcttccg ttgctctcaa aaaatggcat ccgcattccc aggatgaccg
      481 aactgagcgc gaaacgcgcg gcctgggccc acaagtattt ccaggaagaa gtttttccga
      541 tgctgacacc gctcgcggtc gatgccagtc atccattccc gcagctcttg aacaaaagcc
      601 acaacttgct tgtccgcgcc aagacacagc gcggcgggga acggcttcat gcgattgtgc
      661 aggtgccccg tgtgctttca cgcctcatcg cgatgccgcg cggcaaggga gacgatgagc
      721 cctgggttta catttatctg gcttccctca ttaagcagca cattggcgag ttatttccgg
      781 ggctcattct ggacggcgtc cacgcgttcc gcgtcacgcg aaacagcgat ctatacatcg
      841 acgaagagga agcagaaaat ctcttgcgca cgattgaaca agagctgcga cgttccagcc
      901 gcggcaacgc ggtgcgtttg gaagtagagg ctgattgccc gaaagatttc cgcgatttgt
      961 tgctcggctt ttttaatctg acggaagcgg acgtgtacaa gctcgatggc ccgctttcaa
     1021 tgacgcatct tatgccgctc gtgacgaatg atgccttcgc aaatctgcgg gaccggccat
     1081 ttcaaccagc gcgtgatccg gcgcttccgc cgcacgtgaa catttttgag gtgatgcgcc
     1141 gccaggatgt gttgctccat catccctacg aaagtttcga tcctgttgtc gaactcatcg
     1201 aggaggcggc gcaagatccg caggtgctcg cgatcaagat tacgctttat cgcacgagcg
     1261 gcgattcgcc gattattcag gccctgatta acgccgccgt ggcggggaaa caggtcaccg
     1321 ctttagtcga gttacgcgcg cgatttgacg aagctgccaa tattcaatgg gcgcgccagc
     1381 tcgaggaagc cggcgcgcac gtagtttacg gcgtagtcgg tttaaagacc cactgcaaag
     1441 cgcttctcat agtaagacgc gattccgatc ggcttcgtca ttacgtgcat ctgggcactg
     1501 gcaattatca cccgcgcacc gctcgcattt acactgactt tagcctgttc acgtccgagc
     1561 cgcggttaac cgaagaggta tcagtggttt tcaacacgct cactggtctg gccggctatc
     1621 ccggcctgaa aaaattgctc gtcgctccgt tcgatcttca caaacgatta atcggcaaga
     1681 tcgagcgcga gcgcgatcac gcgctggcgg gcacaccagg ccgcatcatt gctaaactca
     1741 atcatctcgt cgaccaggag actatcgaga aactctatga agcttcctgc gccgatgtgt
     1801 ccattgattt ggtcgtccgg ggcatttgtt ccttgcggcc gaaaatcccc gggttgagcg
     1861 aaaacatccg cgtcttcagt attgtgggcc gttttctcga gcacagccgg atttattatt
     1921 ttgagaacgc cggtcagcga gaggtgtttc tctccagcgc cgactggatg ccacgtaatt
     1981 ttttccggcg cgtcgagctg gcgtttccgc tcgaaaaccc aattctgcgc gatgagatcg
     2041 tcaacgaagt gcttccgagt tttttgaacg atcgcgtcaa agcgcgcgaa cttcagccgg
     2101 atggaagcta tcgtcgcttg aaaccagagg gcaaggagcc gcgtgctcag gcgcaatggc
     2161 atttccgcaa agtctcccgc gagcgcgcga aaaaaatgag cggctcgaag aagaaattgc
     2221 gcgccgacaa attgattccg atcacggagg ccgatgcaaa atccgttgcg gctgccgagc
     2281 acgagccggg atcgccgatc tcggctacaa cggggaacca atcctcatga agaaaaagat
     2341 tctcgtaatc gacatcggcg gctcaaacgt gaagctaatg atttcgcgcg cgaaaaaact
     2401 aaaattcgca tccggattca ggttgacgcc gcgcgagatg gtcgcgcaga taaagccttt
     2461 ggtgagcgac tggaaattcg atgcgatttc gatcggattt ccagcgccgg tgcgcgacgg
     2521 gcgaatcctg agcgagccga agcacctgag caaggggtgg atcggattta atttcgaaaa
     2581 agcgtttggc aaaccagtcc gcatcatcaa cgacgcggcc atgcaggctc ttggcagtta
     2641 tcatggccgc cgaatgcttt tcctcgggct cggcacagga cttggctcag cccttgtgtg
     2701 gtacaattat gttctcccgc tcgaactggg agacctgcct tatcgcaacg gcagcatcat
     2761 tgaagattat cttggcaaac cgggcctgac gcggctcggt gagaaaacct ggcggcgcga
     2821 cgtcgagcat gcgctcgtcc agttgaagaa atcactcatc gccgattacg tcgtgctggg
     2881 cggtggcaat gccaaaaaat tagatgcgtt gccagaaggc atcgaacgcg ggcataatcg
     2941 caatgcgttc ctgggcggaa ctcgcctgtg gcaaatcgat tcgcgcacac ggcggccaaa
     3001 atggcagatc ctatgagttc gaataggatt atgaatatga ataagagtgc gaacaagaat
     3061 cctgatcttc tccctcccgg tcttattctt actcgtattc atattcttgc tcgttaggac
     3121 aacattatat ggagctttat ttcctcagac atggggaggc cgattggccg gactggaaaa
     3181 aatcggacga cgaacgtcct ctgactaaac gcggcaagaa agaaatgcac gcagtcgccg
     3241 catttcttgt gcggctcaaa gtgcaaccgg agttaatttt gactagtcca ttgccacgcg
     3301 cggcgcagac ggcagaaatc gcggccgaac acctggatgt gaagtgccgc gaagaacaac
     3361 gtctggcgcc cggattcgga gcggcggagc tggagcaaat aattaacaaa cactccgtgg
     3421 agagtttgat gatcgtcggt cacgaacctg attttacgag aacgatcgct gccttgaccg
     3481 gtgcatcgct gaaactttcg aaggccggcg tcgccctggt cgatctggac gtttcctcgc
     3541 gtagaggcag gctgctctgg cttttcccgc ccaaaatcgc aaagagataa cggttcaacg
     3601 cagcgcaagc agataaattg cgctcagcgc cagcagaaaa tacggaacaa ggactgccgc
     3661 tccggcataa tccttggcca ttcgttgtcc gaagaacaga gcaataagcg caatggcgga
     3721 aatcaccgcg ccataaaatg caattgtcga acctcgtgag a
//
LOCUS       DASQUQ010000176         8032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_315284, whole genome shotgun sequence.
ACCESSION   DASQUQ010000176 DASQUQ010000000
VERSION     DASQUQ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315284"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VE486_05050"
     CDS             <1..534
                     /locus_tag="VE486_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38486.1"
                     /translation="LMQELGRHVSEQTQILFGAAVDGRLGDRLTVTIITSMTADEGLI
                     PQQTQPALSSAPAKLPVWEQPQEIPPQIEIEPTPMEVSEPAEMIPFEEPAPAESPLAP
                     PVEPVVNKPEPIVTTQHKTPAKEEKPSPEKSVQAKQEVLQFEAVTRGRFEKSEPTIVE
                     GEDLDVPTFLRKNIRVK"
     gene            complement(638..1987)
                     /locus_tag="VE486_05055"
     CDS             complement(638..1987)
                     /locus_tag="VE486_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZA38487.1"
                     /translation="MRIRFVSILSLSILLPALGLTKQTPQSLADAELPSLVEIYKDIH
                     SHPELSGHEERTAALIAKELHAAGCEVTENFGKYDDPKLKGYGVIGVMKNGDGPRVLV
                     RTDMDALPVEEETGLPYASKVTTKNDEGKDVHVMHACGHDAHIAAFIGTARALAKLKD
                     QWHGTIVFVAQPAEELGTGARALLKAGLYDRFGKPNFALGFHDKADLETGHIGVTEGY
                     TYANVDSVDITVRGIGGHGAYPHKTKDPIVLAAEIINALQTIASRENNPIDPVVVTVG
                     SIHGGTKHNIIPDEVKMQLTVRTYKSDVRERVLAAIDRIAKACATVAGIPSDRAPIVT
                     VRKDEFTPATYNNPQLTKRLVAVWKKSLGNENVEIVDPTMGGEDFSEYSLPDHSIPAV
                     DFHIGAVDPVKIADSKKTGMPLPSLHSSKFAPVPEPTIRVGVIGMTSAVLELMKQEK"
     gene            2121..3713
                     /gene="aceB"
                     /locus_tag="VE486_05060"
     CDS             2121..3713
                     /gene="aceB"
                     /locus_tag="VE486_05060"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HZA38488.1"
                     /translation="MDKQLISDMPGGIEVRGEITPEFAEILTPDALGFVAKLQRTFRN
                     RRTECLARRQERQAVLDRGATLDFLPETKSIREGDWKCASIPADLQDRRVEITGPTDR
                     KMIINALNSGAKIFMADFEDANSPTWANMMEGQINLRDAVRRTITFTSPQGKEYTLNE
                     NVAVLMPRPRGWHLIEKHLLVDGEPVAGGLFDFGLFFFHNAHELIARGSGPYFYLPKM
                     ESRLEARIWNDAFKLAQDELGIPRGTIRATVLIETIPAAFEMDEILYELREHSAGLNC
                     GRWDYIFSCIKRFRNRPDFLLADRALITMTTHFMHSYSLLCIKTCHHRNTFAMGGMAA
                     QIPVKNNPAANEEAFAKVRADKEREARDGHDGTWVAHPGMVQLATEAFDAMMPQPNQI
                     DRNRDDVQVTAYDLLDFGPSGPITEQGLRTNVSVGVQYLEAWLRGRGAVPLFNLMEDA
                     ATAEISRAQIWQWIRHPRGVLDDGRKVTKELFRRVLDEELGKVKRFAGDKFGLARELF
                     DTITTDDGFAEFLTLPGYEKLD"
     gene            3697..4977
                     /gene="aceA"
                     /locus_tag="VE486_05065"
     CDS             3697..4977
                     /gene="aceA"
                     /locus_tag="VE486_05065"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZA38489.1"
                     /translation="MKNSTEELRGAGAFHTPNARWNGVKRTYSLGDVRRLQGSVIPEH
                     TFARRGAEKFWELLHSEPYVAALGALTGNQAVQQVKAGLNAIYASGWQVAADANLAGQ
                     MYPDQSLYPANSVPALVKRINQALQRADQIAHAEGEMNIDWFVPIVADAEAGFGGPLN
                     VFELIKAMIEAGAAGVHLEDQLASEKKCGHMGGKVLVPTRSAIVNLTAARLAADVCGV
                     PTILIARTDACSAALLTSDVDERDREFITGERTVEGFYRVRAGIDQAIARALCYAPHA
                     DMLWFETSEPDLKAAKKFAESIHAKFPGKLLAYNCSPSFNWKAKLDTTVIIKFQHELG
                     SMGYKFQFVTLAGFHALNYSMFKLAHGYHDRGMAAYSELQEAEFAAERDGYAATKHQR
                     EVGTGYFDEVARIIGGGECSTIALCGSTEQEQFH"
     gene            4974..6761
                     /gene="aceK"
                     /locus_tag="VE486_05070"
     CDS             4974..6761
                     /gene="aceK"
                     /locus_tag="VE486_05070"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HZA38490.1"
                     /translation="MNETFSPACPALTDSRLAALCAEEARGAFHQFETRFDEITRRGR
                     ERFLRRDWRGSFGDAAERLRVYPLALDELMPRIAQLMGRRLHERSIWSAIKAVYSSLI
                     ERSVRREIAESFFNSVTRRVFATEGVDQSIEFVDTDFDTPPQNGAQNVRRAYSGAHLP
                     ELIFRVLTDESVAGFGKQRWNNLQAAAELGAERLRAVLPFATAYEIDILDAVFYRGHG
                     AYIIGRAMAAASRPAIPIAFCLRHPDERGIILDAVLHGEADLAILFSYTRAYFRVDAP
                     CPFEVVRLLRKLMPGKRLADLYNEIGYNRHAKTEFYRDFVGHLENSADRFVAAEGARG
                     MVMLVFTLPSYDVVFKLIRDRFDAPKENDRQDVMQRYRIVFEHDRAGRLVEAHEFEHL
                     RIARDRFDPRLMDELLGEAAGTVRLDKTDVIIAHAYVERRVRPLNLFLRESAAADAVN
                     AARDYGQAIKDLAASNIFPGDLLTKNFGLTRRGRVVFYDYDELCFLTDCNFRDMPQAK
                     SYEEEIAAEPWFSVRGNDIFPEEFPQFLAFPDAARKALLKRHGDLFQFVFWGMFSTSC
                     AGVNCRKFFLTRRNDGWLHNNGSARLERR"
     gene            6831..>8032
                     /locus_tag="VE486_05075"
     CDS             6831..>8032
                     /locus_tag="VE486_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZA38491.1"
                     /translation="MEFEKNTLLFGADRTPGIVAIGLGEAGTVNVYRREKDGSTIADV
                     EPFHPFVWCDSDVVDLGIETEKLESDLKYGWLITVDSWKELIALRNGLKKAGRNFFAF
                     SDPVQHYLTATGRTLFKGLPFEELKRMQVEVLAVETGITDPGYSGHIMSIALSDNTGW
                     EELILIDPNNVDDSERNALKRLASLIKERDPDVIEGHNLFRFDLPYLVARAKKTKAKL
                     DWGRGGGFLRSRPSRLQIAEKTIDYPKFTIDGRHFVDTFLLAQFYDVGMRSLTGFERA
                     DVARHFDLCDSEETSALAGKELQRAYVDNDERFHRRALCGVRETRALSELLSPSYFIQ
                     AQIFPYNYQDVIVRGNATRINALFLREYFRQRHSIPEMPMARTFEGGYTDIFFTGVAR
                     NVWHCDIAS"
BASE COUNT         1761 a         2252 c         2277 g         1742 t
ORIGIN      
        1 ctgatgcagg agcttggccg tcacgtcagc gagcaaaccc agattctctt tggagctgcc
       61 gtggacggac gattgggaga tcgcttgacc gttaccatca tcacttcaat gacggcggat
      121 gaaggtttga ttccgcagca gactcaacct gccctgagca gcgcgccggc gaagcttcca
      181 gtctgggagc aaccacagga gatcccgccc caaatcgaga tcgagccaac cccgatggaa
      241 gtttccgagc ctgccgagat gattccgttt gaggaaccgg cgccagctga aagtccatta
      301 gcgccaccag tggaacccgt ggtcaacaaa ccggagccga tcgtgaccac gcaacacaaa
      361 acgccggcga aggaagaaaa accttcgcct gagaaatcgg tgcaggcaaa acaggaagtt
      421 ttgcaattcg aggcggtcac ccgcggtcga tttgaaaaaa gcgaaccgac catcgttgag
      481 ggcgaagatc tcgacgtgcc gacctttctg cgaaaaaaca ttcgggtgaa gtgagctgga
      541 atgacgaaat ccgaatgacg aatgatgacg aaatccgaat gactaaattc gaaacgacaa
      601 tactctcgtg acgtaatttt tggtcattcg agcttcgtca tttttcttgc ttcatcagct
      661 ccagcaccgc gctcgtcatt ccgatcacgc cgacccggat cgttggctcg ggaacaggcg
      721 cgaacttgct cgaatgcaga ctcggcagcg gcataccggt ctttttgctg tcggcaattt
      781 tcacgggatc gaccgcgcca atgtggaaat caaccgcagg aattgaatga tccggcaggc
      841 tatactcgga aaaatcttcg ccgcccattg ttggatcgac aatctcgacg ttctcgttgc
      901 ctaacgactt cttccaaacc gcaaccagcc gcttggtcag ttgggggttg ttgtaagtcg
      961 cgggcgtgaa ttcatctttg cggacggtga caatgggcgc gcggtcagac gggattcccg
     1021 cgaccgtcgc gcatgctttc gcgatccgat cgatcgctgc cagcacacgt tcacgcacat
     1081 cagatttgta agtgcgcacg gtgagctgca ttttgacttc atccgggata atgttgtgct
     1141 ttgtgccgcc gtgaatggag ccaaccgtca caacgaccgg atcgatcgga ttattctcgc
     1201 ggctcgcgat ggtttgcagc gcattaatga tttcggccgc caagacaatc gggtctttag
     1261 ttttgtgcgg ataagcaccg tggcctccga tcccgcgcac agtgatgtcc acagaatcga
     1321 cattcgcgta agtgtaacct tcagttacgc cgatgtggcc ggtttccaga tcggccttgt
     1381 catggaaacc gagtgcgaaa ttcggtttgc cgaaccgatc gtaaagccct gctttgagca
     1441 aggcgcgcgc gccggtcccc aattcctcgg ccggttgggc cacaaacacg atcgtgccgt
     1501 gccattgatc ttttagcttc gcgagcgcgc gcgctgtgcc aatgaacgca gcaatgtgtg
     1561 catcgtgtcc gcaggcgtgc atcacgtgga catctttgcc ttcgtcattt ttcgttgtga
     1621 ctttgctcgc gtaaggcaaa ccggtttctt cttccaccgg caacgcgtcc atgtccgtgc
     1681 gcacaaggac tctcggtcca tcgccattct tcataacgcc gatcacgccg tagcccttga
     1741 gtttcggatc gtcgtatttg ccaaaattct cagtgacctc gcacccggca gcgtgcagtt
     1801 cctttgcgat tagcgctgcg gtgcgttcct catgccccga caattccgga tggctgtgaa
     1861 tgtctttgta aatctcgacc aaggagggca gctccgcatc ggccagtgac tgcggcgttt
     1921 gcttggtgag gccaagcgcc ggaagcagga tggacagcga caagattgag acgaaacgaa
     1981 ttctcatgct tgtatgctga gcgaaagccc gccctgagcg caagtcgaat ggctcgaacc
     2041 atctctcaac ttgaattcca tggagatgat aataagagat tcctcgattt cgctcggaat
     2101 aacaaaagct tcttgtcaca atggacaaac aattgattag cgatatgcca ggagggatcg
     2161 aagtgcgcgg cgagatcacg ccggagtttg cggaaatcct aacgcccgat gcgctcggtt
     2221 ttgttgcgaa gttgcagcgc acgttccgaa accggcgaac tgaatgcttg gcacgacgcc
     2281 aagagcgtca ggctgtgctc gatcgcggcg cgacgctcga ttttctgccg gagactaaat
     2341 cgattcgtga aggcgattgg aaatgcgctt ccataccggc cgacctccag gatcggcgcg
     2401 ttgagatcac tggcccgacc gaccgcaaga tgatcatcaa cgcgctcaat tcaggcgcga
     2461 agatattcat ggccgatttt gaggacgcga actctccgac ctgggccaac atgatggaag
     2521 gtcagatcaa tctgcgcgac gctgtccggc ggactatcac gttcacaagt ccccagggca
     2581 aagaatacac cctgaacgaa aacgttgcgg tactaatgcc acgtccgcgc gggtggcatc
     2641 ttatcgaaaa acatctgctc gttgatggcg aaccggtcgc cggcggactg ttcgatttcg
     2701 gtctcttttt cttccataac gcgcacgagc tgatcgcgcg cggtagtggc ccttattttt
     2761 atttgccgaa aatggagagc cgcctcgagg cgcgcatctg gaacgatgct ttcaaactgg
     2821 cacaggacga gctcggcata ccgcggggaa cgattcgcgc gaccgtgctc atcgagacca
     2881 tcccggccgc gttcgaaatg gacgagatcc tctacgaact gcgtgaacat tccgccggac
     2941 tgaactgcgg ccgctgggat tacatttttt cctgcataaa aagattcagg aacaggccgg
     3001 attttcttct ggcggaccga gccctgatta cgatgacaac gcacttcatg cattcctatt
     3061 ccctgctttg catcaagacc tgtcatcacc ggaacacttt cgccatgggc ggcatggcag
     3121 cgcagattcc ggtgaaaaat aatccagccg ccaacgagga agctttcgcc aaagtccgtg
     3181 ccgataagga acgcgaagct cgcgatggcc acgacggcac ctgggtcgcg catcctggaa
     3241 tggttcagct cgccaccgaa gcattcgatg cgatgatgcc gcagcctaac caaatcgacc
     3301 gcaatcgcga cgacgtgcag gtgactgcgt atgacctgct cgatttcggt cccagcgggc
     3361 cgatcacgga acaaggcctg cgcaccaatg tgagcgtggg cgtgcaatat ctcgaagcat
     3421 ggctgcgtgg acgtggcgca gtcccattgt ttaatctcat ggaagatgct gcgacggccg
     3481 aaatttcgcg agcccaaatc tggcaatgga ttcggcatcc gcggggggtt ctcgacgacg
     3541 ggcgcaaggt aacaaaagag ttgttccgcc gcgtgctcga tgaagaactt ggcaaggtca
     3601 aacggtttgc cggcgacaag ttcgggcttg cgcgagaact gttcgacaca attacgaccg
     3661 atgatggatt cgcggaattc ctgacgctgc cgggctatga aaaactcgac tgaagaatta
     3721 cgaggggcgg gggctttcca cacgccgaac gcgcgatgga acggtgtgaa acgcacatat
     3781 tccctcgggg acgtgcggcg attgcagggc agcgtcattc ccgagcacac atttgcgcgc
     3841 cgtggggcgg agaagttttg ggagttgctc cattccgaac cctacgtcgc cgcgcttggg
     3901 gcgcttactg gcaatcaagc cgtgcaacag gtgaaagcgg gcctgaacgc aatctacgcg
     3961 agtggctggc aggtcgcagc cgacgcgaat cttgccggac agatgtatcc cgatcaaagc
     4021 ttgtacccgg caaatagcgt gccggcgctc gtgaagcgca tcaaccaggc gttgcaacgc
     4081 gcggatcaaa tcgcgcatgc cgaaggcgag atgaacatcg attggtttgt gccgattgtt
     4141 gctgacgccg aagccggatt cggcggcccg cttaacgtgt ttgagttgat caaagccatg
     4201 atcgaagccg gtgcggctgg cgtgcacttg gaagatcagc tcgccagcga aaagaagtgt
     4261 ggccacatgg gcggcaaagt gctggtgccg acgcgcagcg cgatagtcaa tctcactgct
     4321 gcgcgacttg ccgccgatgt ttgtggcgtg ccgacaattc ttattgcgcg cacggacgca
     4381 tgcagcgccg cgttattgac gagcgacgtg gacgagcggg accgggaatt catcaccggt
     4441 gagcgaacag tcgaaggatt ttaccgcgtt cgggccggta tcgatcaggc catcgcccgc
     4501 gcgctgtgct acgcgccgca cgccgacatg ctttggttcg aaacgtcgga gcctgacctc
     4561 aaggcagcga agaagttcgc cgagagtatc catgcaaagt tcccgggcaa actgctcgcc
     4621 tataactgct cgccatcttt caactggaaa gcgaaactcg acaccaccgt gatcatcaaa
     4681 tttcagcacg agctcggctc gatgggctat aaatttcaat tcgtcacgct ggctggtttt
     4741 cacgcgctta actacagcat gtttaagctc gcgcacggct accacgaccg cggcatggcg
     4801 gcctattccg aattgcagga agcggagttt gccgctgaaa gagatggcta cgccgcaacc
     4861 aaacatcagc gcgaggttgg caccggctat ttcgacgaag tcgcgcgaat catcggcggc
     4921 ggcgaatgct ccaccatcgc gctgtgcggc tcaaccgaac aagagcagtt tcattgaacg
     4981 agacgttttc gcctgcctgc ccagcactga ccgacagccg gctggcagcg ctttgcgccg
     5041 aagaggcgcg cggcgcgttt caccagttcg aaacgcgctt tgacgagatc acgcggcgcg
     5101 ggcgcgagcg ctttctcagg cgggactggc ggggcagttt cggtgatgcc gccgagcgac
     5161 tccgggttta cccgctcgca ttagatgagc tgatgccacg aatcgcacag ctcatgggcc
     5221 ggcgtttgca cgagcgctca atctggagcg cgatcaaggc ggtttattca tcacttatcg
     5281 agcgctcggt tcgccgggaa attgcagaga gctttttcaa ttcagttacc cggcgagtct
     5341 tcgcgacaga gggcgtcgat cagtcgattg aattcgttga caccgacttc gacacgccgc
     5401 cgcaaaatgg cgcccagaat gttcgccgtg cctattctgg cgcgcatctc ccggaattga
     5461 tctttcgcgt gctaactgat gaatcggtcg ccggattcgg caagcaacgc tggaacaatc
     5521 tgcaagccgc cgctgaactc ggggcggaac gccttagggc tgttctacca ttcgccacgg
     5581 catacgagat cgacattctc gacgcggtct tttatcgcgg gcatggcgca tacatcattg
     5641 ggcgcgctat ggccgccgct agccgtcctg caattccgat cgcgttttgt ctgcgtcatc
     5701 ccgatgagcg tggcatcatc ctcgatgcag tgctccacgg cgaggcagac cttgcgatct
     5761 tattttccta cacgcgcgcc tattttcgcg tcgatgcgcc atgtccattc gaagtcgtgc
     5821 ggttgctgcg gaagctcatg ccgggcaaac gcctggccga tctctacaac gagatcggtt
     5881 acaaccggca tgcgaagacg gaattctacc gcgatttcgt cggccatctt gaaaattccg
     5941 ccgatcggtt cgtcgcggca gagggcgctc gcggaatggt gatgctcgtt ttcactctgc
     6001 cgagttacga tgttgttttt aaactgatcc gcgaccggtt tgatgccccg aaagaaaacg
     6061 accgacagga cgtgatgcag cgctatcgca ttgttttcga acacgaccgc gccggacgtc
     6121 tggtggaggc gcacgagttc gaacacctcc gcatcgcgcg tgatcgtttt gatccgagat
     6181 tgatggatga gctgctcggc gaggccgcgg gcacggtgcg gctggacaaa acagatgtga
     6241 ttatcgccca cgcttatgtc gaacgccgcg tccgcccgct caatcttttc cttcgggaat
     6301 ccgccgccgc tgatgccgtt aacgcagcac gcgactatgg acaggcgatt aaagatctcg
     6361 cggcgtcgaa tatttttccc ggcgatctcc tgacgaagaa tttcggcctc actcgccgag
     6421 gccgagttgt attctacgat tacgacgagc tttgttttct caccgattgc aattttcgcg
     6481 acatgccgca ggccaaaagc tatgaggaag aaatcgcggc tgagccatgg ttttctgtcc
     6541 ggggtaacga tattttcccg gaagaatttc cgcagtttct ggcctttccg gatgccgcgc
     6601 ggaaagcact gctaaaacgg cacggcgacc tgtttcaatt cgttttctgg ggaatgttca
     6661 gcacaagctg cgcgggggtg aactgccgga aatttttcct tactcgcagg aacgacggtt
     6721 ggctccacaa taacgggagc gctcgcctgg aaagaaggta gccattttaa cggctcgatc
     6781 agagcctcgg ttttgaatct gcgaaaatct gggcaatctg tggatgactg atggaatttg
     6841 agaaaaacac gctgctcttt ggcgctgacc gaaccccggg cattgttgcc atcgggttgg
     6901 gcgaggccgg cacggtcaat gtttatcgcc gggaaaaaga tggatcgacc attgccgacg
     6961 tcgagccgtt ccatccgttc gtttggtgcg actcggacgt tgtcgatctc ggcatcgaaa
     7021 ccgaaaagct cgagagcgat cttaaatacg gctggttaat tacggtcgat agttggaagg
     7081 aattgatcgc gctgcgcaac ggtctgaaaa aggctgggcg caacttcttc gcctttagcg
     7141 acccggttca gcattacctc accgccaccg gccgcacatt attcaaagga ctgccgtttg
     7201 aagaattgaa gcggatgcag gtcgaagtgc tggccgtcga aaccgggatc accgatcccg
     7261 gctacagcgg ccacatcatg agcatcgcac tctcggataa taccggctgg gaagaactga
     7321 tcctcatcga tccgaacaac gttgacgact ccgagagaaa cgcgctcaaa cgactcgcct
     7381 cgctgatcaa agaacgcgat ccggatgtga tcgaaggtca taaccttttc cggttcgatc
     7441 tgccgtatct ggttgcgcgc gcgaagaaga cgaaagcgaa actggattgg ggacgcggag
     7501 gcggttttct gcgctcgcgt ccctcgcgtc tccagatcgc tgagaagacg atcgattacc
     7561 cgaagttcac catcgatggc cgccattttg tcgatacgtt tttgctcgca caattttacg
     7621 atgtcggaat gcgttcccta accgggttcg aacgggccga cgtcgcgcgc cattttgatc
     7681 tttgcgatag cgaggaaacc tctgcgcttg ctggcaaaga gttacaacgc gcatacgtgg
     7741 acaatgacga gcgctttcat cgtcgcgcgc tctgtggggt ccgcgaaacg cgtgctttat
     7801 cggaactcct cagtccgagc tacttcatcc aggcacagat ttttccctac aattatcagg
     7861 acgtgatcgt gcgcggcaac gctacccgga tcaatgcgct attcctgcgc gaatatttcc
     7921 ggcaacgcca ttccattccc gaaatgccaa tggcgcgaac gttcgaaggc ggttacaccg
     7981 atatcttttt caccggcgtg gcgcgcaacg tctggcattg cgacatcgcc tc
//
LOCUS       DASQUQ010000177         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_857013, whole genome shotgun sequence.
ACCESSION   DASQUQ010000177 DASQUQ010000000
VERSION     DASQUQ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857013"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VE486_05080"
     CDS             complement(<1..461)
                     /locus_tag="VE486_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZA38492.1"
                     /translation="MTSIWELANPQLRDLTVYEPGKPIEETARELGVEPGAIVKLASN
                     ENPLGPSPKAIQAIRAAAENAHLYPDGGGFYLRKAIAARLGLGLENIILGNGSNEVLE
                     FLGHAFLDLNDDVITSEYAFIVYKLIATSFGARTIEAPSPDYQQDLEAMLNA"
     gene            557..1732
                     /locus_tag="VE486_05085"
     CDS             557..1732
                     /locus_tag="VE486_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZA38493.1"
                     /translation="MADDELIYLDNNATTPLDPAVIEEMLPFLTKYCGNPSAGYLFAA
                     QARRAVDLARDRLARLLGCESSEIVFTSGGTESNNAVINSALQFEPRGKHVVTSAVEH
                     SAVLHPCQDLTKRGCDVTFLGVDRHGNVDPGELEAAIRPETALVSMMWANNETGVLFP
                     VEKIAEICREKGVLFHTDAVQATGKIPIRLRDTAINFLSLSAHKFHGPKGVGALYIRR
                     GTRFHPLIAGGGQENGRRGGTENVASIAGLGKAAERAVEFLSEDKGHVRSMRDRFEKA
                     IVQTVSGASVNGAGAARVPNTSSFAFEGIESSAALLLLDRHRVCCSAGSACRTGSQEA
                     SHVLRAMNPSGDGARRSLRFSFGRFNTDAQVDRAIEVVPKVIEKLRQTASPAVATAL"
     gene            1887..>2318
                     /locus_tag="VE486_05090"
     CDS             1887..>2318
                     /locus_tag="VE486_05090"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HZA38494.1"
                     /translation="METPRDPAASRYEHAAQGLRRSDLDPDPIKQFSNWFTAAIEAGI
                     RDVNAMSLATTAKECKPSVRIVLLKGFDHDGFVFFTNYESEKGKQLQANPHAALAFYW
                     IELDRQIRISGKAEKTSREESQTYFHSRPLGSQLSAWASRQS"
BASE COUNT          506 a          631 c          678 g          503 t
ORIGIN      
        1 gcattcagca tcgcctcgag atcctgctga taatccgggc tcggcgcttc aattgtgcgc
       61 gcgccgaaag aggtcgcgat caatttatag acgataaagg catattcgga cgtgatcacg
      121 tcatcgttga ggtcgagaaa ggcatggccg agaaattcga gcacttcgtt cgagccgtta
      181 cctaaaataa tattctccaa accaagaccg agtctggccg cgattgcctt gcgcaggtaa
      241 aatccgccgc catctggata caggtgcgcg ttttccgctg cggcgcgaat tgcttgaatt
      301 gcttttggag acgggccaag cggattctcg tttgaagcca gcttgacgat cgcgcctggt
      361 tctacgccga gttcgcgcgc cgtttcctcg atcggttttc ccggttcata aacggtgaga
      421 tctcgcaatt ggggattcgc cagttcccag atcgaagtca tgcgcgcaat ctagacagaa
      481 ttaggagaat cgacaaaacg cggatcgcgc gaacgcggag attgcagcgg gcccggctgt
      541 ctctacaatg gagttgatgg cagacgacga actcatttat ctcgataaca acgccaccac
      601 gccgctcgac cctgcggtaa tcgaggaaat gctgccattc ttgacgaagt attgcggcaa
      661 tccttcggcc ggctacctct ttgctgccca ggcgcgaaga gctgtcgatt tggcccgtga
      721 ccggctggcg aggttgctcg ggtgtgaatc gtcggaaatt gttttcacca gcggcggaac
      781 ggagtcgaac aacgcggtga tcaattcagc gctccagttt gagccgcgcg gcaagcatgt
      841 ggttacgagc gcggtggaac acagcgcggt gctgcacccg tgccaggatc tcacaaagcg
      901 agggtgcgac gtgacattcc tcggcgtgga tcgacacggc aacgtcgatc ctggcgaatt
      961 ggaggcggcg attcgccccg aaacggcact cgtttcgatg atgtgggcaa ataatgaaac
     1021 gggggttcta tttcctgtcg agaaaatcgc agagatatgc cgcgagaaag gcgtgctctt
     1081 tcataccgac gccgtgcagg cgaccggaaa gatcccgatc cgcctgcgcg atacagcgat
     1141 taatttccta tccctctctg ctcacaaatt tcacgggcca aaaggcgtcg gcgcgcttta
     1201 tatccgtcgc ggaacacgtt tccacccatt gattgctggt ggcggtcagg aaaacgggcg
     1261 acgcggtgga acagaaaacg tcgcctcgat tgccggtctg gggaaagcgg ccgagcgagc
     1321 tgtcgaattt ctgtcggagg acaaggggca tgttcgttcc atgcgcgacc ggtttgaaaa
     1381 agcgattgtg caaacggtga gtggagcatc ggttaacggc gcgggcgccg cacgagttcc
     1441 aaacacgtcg agtttcgcat ttgaagggat cgagtcgtca gcggcccttc ttttattgga
     1501 tcggcatcgc gtttgttgtt cagccggatc agcctgccgg acgggctcgc aggaagcttc
     1561 gcatgtgctg cgcgcaatga atccgagcgg agacggcgcg cggcgcagtt tgcgtttttc
     1621 ttttggccgg ttcaacaccg atgcgcaggt cgatcgcgcc attgaagtcg ttccgaaagt
     1681 tatcgaaaaa ctgcgtcaga cggcctcgcc ggctgtagcc acggctctgt gagccgttct
     1741 tctttcgtga gaccagcgct gatgtcgacc ggccactggt ccgcacaccg gacggattcg
     1801 tcctgcggcg aaccggtggc tacacaaatc gctgcatcca caatttttct tgcggcgcaa
     1861 tatagtgcgc gattgttctt ttcgtgatgg agacgccacg agatccggcc gcttcgcgtt
     1921 acgagcatgc cgctcagggc ttgcggcgga gcgatctcga tcccgatccg atcaaacaat
     1981 tttcaaactg gttcacggcg gcgatcgagg ccgggattcg cgatgttaac gcaatgagcc
     2041 tcgctactac cgcgaaggaa tgcaaaccat cagtgcggat tgttctctta aaaggtttcg
     2101 atcacgacgg tttcgtcttt tttaccaact acgaaagcga aaagggaaag cagctccaag
     2161 ccaatcccca tgcggcactg gcattttact ggattgaact ggaccgccaa attcgcatca
     2221 gcgggaaagc ggaaaaaacc tcgcgtgaag agtcacaaac ctattttcac tcgcgcccgc
     2281 tgggcagcca gttgagcgcg tgggcctcgc gccaaagc
//
LOCUS       DASQUQ010000178         3934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1126678, whole genome shotgun sequence.
ACCESSION   DASQUQ010000178 DASQUQ010000000
VERSION     DASQUQ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126678"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VE486_05095"
     CDS             complement(<1..1104)
                     /locus_tag="VE486_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZA38495.1"
                     /translation="MKTFTQISLAAVIAPLCCAATDATSVDALVAEAFARNPEVNVYR
                     AQIAAAKGERRTAAEWQNPELTTDIGAKLVRDLDGRSLGNGQIWTVSAMQTFEYPGRI
                     ALRKAIASHQIALAELGLEGFRVALAARVRSLLYRAALSEQKANAATAVAKRFDDLVS
                     VLSQRPIAGVAPQLDVRIIEASAIALKRRPIEAQREIQSAIYELNQLRGAKPNTPLNI
                     PQMNLNLSPIASAAALIASARTNNYRIRTHVVELEQQGFKVKLALNERWPAVRVGPYA
                     HNERADTNEYHFGVGATLPLPLWNKNAGKIETAKARAAQAEAELTAMIREVERKVSDA
                     AFIYGSRRDEVEKLQVSILPQMREAADVADRNYR"
     gene            complement(1206..1628)
                     /locus_tag="VE486_05100"
     CDS             complement(1206..1628)
                     /locus_tag="VE486_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38496.1"
                     /translation="MIAALVICAPIACSEEILPQLIAQIASLDHDHGIFLHATSDGVD
                     LILTHHAHVTRAGDEGRSLDESQPAHVIHMISGSATVKQSASLIVSNAKQLVAYFPTA
                     ITAEWRTFVPRLPLAYSRPPPEAMILRLDRSALAGLLI"
     gene            complement(1765..2499)
                     /locus_tag="VE486_05105"
     CDS             complement(1765..2499)
                     /locus_tag="VE486_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZA38497.1"
                     /translation="MIRTHCFFVVGFLTTIVSIQAGPPFVTDDPEPPPPGGWEINVPF
                     IIERTPGETEMNAPLFDLNYGLPNTQLKLEIPVEIIHDDSDGTVSGAGDLLVGVKWRF
                     LNKEQSQLQVAIYPQLLVPTGDHTRGLGQGGSAFVFPLLAQKNWDKWTLYGNVGYWWQ
                     AGAETRDYVYAGAVLEREINERLTLGMELFGNSPKERGRRSELAFNVGGSWKLGKHFN
                     LLFAGGRDLVGDTTAMAYIGLQLLTK"
     gene            complement(2538..3482)
                     /locus_tag="VE486_05110"
     CDS             complement(2538..3482)
                     /locus_tag="VE486_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZA38498.1"
                     /translation="MPTVSAQNAAPLKLKEIIALPGVEGRIDHFAFDAAGERLFVCAL
                     GNNTVEGLDLRNGQRIHSITGLGAPQGIAYIPELDRMFVANDKGGICKIYDGKSFQVI
                     GELNFKDDADNIRYDEATKKIYIGFGSGGIAIVNAPDGKQIGSIKLSAHPEAFALEKQ
                     GKRIFVNVPDARHVAVIDREKGEVVNRWKTDLAFGNFPMALDEANHRLFLGCRLPSKL
                     LVLNTDSGEVVAKADISGDSDDIFYDSKRHRIYAICGAGKIDIIEQTDPNTYTVSTKV
                     DTADGARTGFFAPEHDTLFVAVPHRGSQQAEIRAYHVQ"
     gene            complement(3539..>3934)
                     /locus_tag="VE486_05115"
     CDS             complement(3539..>3934)
                     /locus_tag="VE486_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HZA38499.1"
                     /translation="SAGPLNQILGVAGEVNNGMVKFTIGRPAKMDGVTIGKDMGVNTW
                     AAFAGSDDNAVVDGDFAVTEDELQPVLKSLLKEKINIVAIHQHMTHEEPRIMFFHYWG
                     RGTAKDLAQAIKGGFLVGGLLGVSSPLQN"
BASE COUNT          867 a         1127 c         1028 g          912 t
ORIGIN      
        1 tctgtaattg cggtctgcca catccgccgc ttcgcgcatt tgcggcaaga tcgacacctg
       61 caatttctcc acttcgtcgc gccgcgatcc gtaaatgaac gcggcgtcgg agaccttccg
      121 ctcgacttct cgaatcattg ctgtcagttc agcttccgcc tgcgcggcgc gcgctttcgc
      181 ggtctcgatt ttgcctgcgt tcttattcca aagcggcaac ggcaaagtcg caccgacacc
      241 gaaatgatat tcgtttgtgt cagcgcgttc gttatgtgcg tacggcccca cgcgcacagc
      301 cggccagcgt tcattgagcg cgagcttcac tttgaagccc tgttgctcca gctcgaccac
      361 atgcgtgcgg atccggtaat tgttcgtgcg tgcgcttgcg ataagtgccg cagccgacgc
      421 aattggcgat agattcaaat tcatttgcgg gatgttcaac ggcgtattcg gtttcgcccc
      481 gcgcagttgg ttcagttcgt agatcgcgct ttgaatctcg cgctgtgcct caatcggccg
      541 gcgtttcagg gcaatggcgc tcgcttcgat aatccgcacg tcgagctgtg gcgcgacacc
      601 tgcgatagga cgttgcgaca agaccgacac caggtcatca aagcgcttcg ctaccgccgt
      661 ggctgcattc gctttctgct cgctcaacgc ggcgcggtac aagagcgagc gaacccgtgc
      721 cgctaatgcc acgcgaaatc cctcgagtcc cagttccgca agcgcgattt gatgactggc
      781 gatcgctttg cgcagcgcaa tccggccggg atactcaaag gtttgcattg ccgaaaccgt
      841 ccatatctga ccgttgccta acgaacgacc atcaaggtcg cgcaccagtt ttgcgccgat
      901 atcggtcgtt aattccgggt tttgccattc agcggctgtc cgacgctcgc ctttggccgc
      961 ggctatttgg gcacgataaa cattcacttc cggattccgc gcaaatgctt ccgcaaccag
     1021 cgcatcgacc gaagtcgcat ccgtagccgc acaacacagc ggagcgatca ccgctgccag
     1081 gctgatctga gtaaacgttt tcaattttaa aaggagatgt tgagcaaagg aattctccgg
     1141 gcacgacaca cgtgcacgga cccgcgaaag aaccgccgaa cgctttcagg gttcgcgaat
     1201 ttgaatcaga tcagcagtcc cgcgagcgcg gaacgatcga gtcgcaagat catcgcctcc
     1261 ggcggcggac gcgaataagc gagcggcagt cgtggaacga atgtccgcca ttccgcggtg
     1321 atagccgttg gaaagtaggc aacgagttgt ttcgcattgc taacaatcag ggacgcggat
     1381 tgttttaccg tagcagatcc actgatcatg tgaatcacgt gcgccggttg tgattcatcg
     1441 agagaccgcc cttcgtcacc cgcacgcgtg acatgcgcgt ggtgagtaag aatcaaatcc
     1501 actccatcgc tcgttgcgtg caagaaaatg ccatgatcgt gatcaagcga ggcgatctgt
     1561 gcaatcagct gtggcaagat ttcttcgctg caagctatcg gagcgcagat cacgagtgcc
     1621 gcaatcaatt ggcaccacct cggccgccgg ctcatgcgct tcaacacggg aggagcgtgc
     1681 tcactcgctt acagaagtca acacggaaga cacgcgaatg ctgtacgaaa atccgccttc
     1741 cgaacctgaa tcaagtggcg aaattcactt tgtaagaagc tgcaacccga tgtacgccat
     1801 cgcagttgta tcgccgacaa gatcgcgtcc accagcgaag agcaggttga agtgtttgcc
     1861 caacttccaa ctgccgccga cgttgaacgc cagctcggaa cggcggccac gttctttggg
     1921 tgaattgccg aacaactcca tgccaagcgt cagccgctca ttgatttcac gttccagcac
     1981 ggcgccggcg taaacgtagt cgcgcgtttc agcgccggct tgccaccagt agccgacgtt
     2041 gccgtaaagc gtccatttgt cccagttttt ctgcgcgagt aacgggaata cgaacgcaga
     2101 tccaccctgt ccgaggccac gggtgtgatc acctgttggc accaacaact gcgggtagat
     2161 ggcaacttga agttgcgact gctctttgtt caaaaagcgc cacttcaccc cgaccagcag
     2221 atcgcctgcg ccactgacgg tgccatcgct gtcgtcgtga atgatttcga cgggaatttc
     2281 cagtttgagt tgtgtattcg gtaacccgta attcaaatcg aagaggggcg cattcatctc
     2341 cgtttcgcct ggagtacgct caatgatgaa cggaacatta atttcccatc cgccgggtgg
     2401 aggcggctcc gggtcatcgg tcacgaacgg tggcccggct tggatcgaaa cgattgttgt
     2461 caggaaaccg acgacgaaaa aacagtgcgt cctgatcatc taacctgcgt ctgccgcatt
     2521 cgataggcta tcttcaatta ttggacgtga taagcgcgaa tctccgcctg ttgcgaacca
     2581 cgatgcggca cagcaacaaa caaggtatcg tgctccggcg caaaaaatcc ggtccgagcg
     2641 ccgtctgctg tgtcgacctt agttgagact gtataggtat tcggatcagt ctgctcgatg
     2701 atgtcgattt ttcccgcgcc acagatcgcg tatatgcgat ggcgcttgct gtcgtaaaaa
     2761 atgtcatcgg aatcgccgga aatgtcggcc tttgcaacga cctcacccga atccgtgttc
     2821 agcacgagaa gctttgatgg aaggcggcat ccaagaaata atcgatgatt cgcttcgtcg
     2881 agcgccatag gaaaatttcc aaatgccaga tctgtcttcc atctgttaac tacttcgcct
     2941 ttctcgcgat cgatcactgc cacgtgcctg gcatcgggaa cgtttacgaa gatccgcttt
     3001 ccctgctttt ccagcgcgaa cgcttctgga tgagccgaaa gtttaattga gccaatttgc
     3061 ttcccatcgg gcgcgttaac aatggcgatt ccgccgcttc caaatccgat ataaatcttt
     3121 ttcgtcgctt catcgtaacg gatgttgtca gcgtcatctt taaaatttag ttccccgata
     3181 acctgaaatg attttccatc gtagatcttg caaatgccac ctttgtcgtt tgcaacgaac
     3241 atacgatcga gttcggggat gtaggcaatt ccctgtggcg caccgagacc cgtgatcgaa
     3301 tgaattcgtt ggccattgcg caagtcgagg ccttcgacgg tgttattgcc gagcgcacaa
     3361 acaaacaatc gttcgccagc ggcatcaaac gcgaaatggt cgatccgccc ttcgacgccg
     3421 gggagcgcga ttatctcttt taatttgagc ggcgccgcgt tctgtgcaga tacggtgggc
     3481 accgctgtgc aacacagcgc gacagcaacg taaacggtct tactggacaa ggacgcgctc
     3541 aattttgtaa tggtgagctg acgccaagca atccgcccac caagaacccg cctttgatag
     3601 cttgggccaa gtctttagcc gtgcctctac cccaataatg gaagaacatg atgcgcggct
     3661 cttcatgagt catgtgttga tggatggcga caatgttgat cttttcctta agcaacgatt
     3721 tcaacaccgg ttgcagttca tcctccgtca ccgcgaagtc gccatcaacc acggcgttat
     3781 cgtcggagcc tgcgaaagcc gcccatgtgt tgacgcccat gtctttgccg attgtcacgc
     3841 cgtccatttt tgccggacga ccgatggtaa atttgaccat tccgttgttc acttcgccag
     3901 ctactccgag aatctgattg agcggcccgg ccga
//
LOCUS       DASQUQ010000179         2803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_39440, whole genome shotgun sequence.
ACCESSION   DASQUQ010000179 DASQUQ010000000
VERSION     DASQUQ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39440"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VE486_05120"
     CDS             <1..1024
                     /locus_tag="VE486_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZA38500.1"
                     /translation="LAYSTVSQLGLMMVSLGVGGVAAGIMHLLAHGFFKALLFLGAGS
                     VIHGCQGEHDIRKMGGLRRPMRVTFLAYAVGMMALSGVPLFFSGGWTKEEILHATAHW
                     PKSQVPYYLLLAGVVLTALYMTRQIIYVFFGKSRAASEHAHESPRVMTMPLTALALGA
                     IFLSVVLTPTWPWLHDYLTGESAHFDIARLIQPALFVSLALVAAGIGLGVLMYRNAGE
                     TDPLKQSQPALFRFLENKMWLDELYDRTVIAFATICARVSDWMDRYFWDGLVRAFGGL
                     GQLFGIFTTSVDERGINAGVDETTTGTRGLGRVMSGWHSGQIQTYLGIVAIGMLALLL
                     LYAWLV"
     gene            1012..2487
                     /locus_tag="VE486_05125"
     CDS             1012..2487
                     /locus_tag="VE486_05125"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZA38501.1"
                     /translation="MAGLITALFLVPLVGALFVSVAPKSYARGIALACNAITATLAFT
                     TWRNFDTGATGLQMIERHTWIPAIGAEYLLGIDGLSLLLVLLTSVIIPFAFLAQRMTR
                     GFCAIMLILQSALYGTFTAQNFVLWFLFYEMSLIPAFLLIKIWGGQNRDRAATKFFLY
                     TFLGSVAMLLSFLGIYFATGTFDFAGLADLGKNGLLTGKVAWFAFAGIFLGLAVKVPL
                     FPFHTWLPDAYETAPTGVSMVLTGVLSKMGVYGFVRLLLPLFPNEIKILGPWLLGLAV
                     CSIVFASLAAWAQSDLKRMVAYLSINHLGYCMLGLFAVTVSPIVAARIDTQAALSGAF
                     MQIFNHGITAAALFYYVGLLEQRRGLRGINDFGGLMQRTPLLCGWMSVAMFSSLGLPG
                     LNGFIGEFLIFKGSFAIAASFTAVAVIGLLVTAIAFVRAMQKLFSGPLVESCSAFPDL
                     LPTEKLVVVPVTLLMFAIGIAPQFVFNIFNTTVIQMARLFA"
     gene            2568..>2803
                     /locus_tag="VE486_05130"
     CDS             2568..>2803
                     /locus_tag="VE486_05130"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HZA38502.1"
                     /translation="MLDSDLATLYGVTTGNLNKAVKRNADRFPSDFMFRLKPEELANL
                     KFQFGISSWGGRRRSRPYVFTEQGIAMLSSVLNS"
BASE COUNT          495 a          809 c          781 g          718 t
ORIGIN      
        1 tctcgcttac tcaactgtgt cgcaacttgg tttgatgatg gtttcgctcg gcgtgggcgg
       61 tgtcgcagcc ggcatcatgc atctgctcgc gcacggtttt ttcaaagcgc tactgttcct
      121 cggcgcaggg tcagtgattc atgggtgtca gggcgaacac gacattcgca aaatgggcgg
      181 cttacgccgg cccatgcggg tgacattcct cgcatacgcc gtcggcatga tggcactcag
      241 cggtgtgccg ctcttttttt cgggtggctg gaccaaggaa gaaattctgc acgccacggc
      301 gcattggccg aagtcgcagg tgccgtatta cctgttgctc gccggagtcg ttttgaccgc
      361 gctctacatg acgcgccaga tcatctatgt ctttttcgga aagtcgcgcg ctgcttccga
      421 gcacgcgcac gaaagtccgc gcgtgatgac aatgccactg acagcgctcg cgctgggcgc
      481 aattttcttg agcgtcgtgc tcactccgac ttggccgtgg ctccacgatt atctaactgg
      541 cgaatctgcg cactttgaca tcgcgcggtt aattcaaccg gcgcttttcg tttcgctcgc
      601 gctcgtcgcg gccggcatcg ggctcggcgt tcttatgtat cgcaatgccg gcgaaaccga
      661 cccgctcaag caatcgcagc ccgcactctt tcgctttctc gaaaataaaa tgtggctgga
      721 cgaactttac gatcggaccg ttatcgcttt cgccacgatt tgcgcacgcg tctccgattg
      781 gatggatcgt tatttctggg acggtctcgt gcgcgctttc gggggtctcg gtcagctttt
      841 cggaattttt acaaccagcg tcgatgagcg cggcatcaac gccggagtcg acgaaacaac
      901 gaccggcacg cgcggcctgg gccgagtgat gtcgggttgg cactcggggc aaatccaaac
      961 ttatctcggg atcgtcgcca tcggcatgct cgcgctgctc cttctttacg catggctggt
     1021 ttaatcacgg cgctgtttct cgtcccgctt gtcggcgctc ttttcgtgag tgtggcgccg
     1081 aaaagctatg cgcgcggaat cgctctcgcg tgcaacgcca tcaccgcgac cctcgcattc
     1141 acgacttggc ggaatttcga cacgggcgcc accggtctcc agatgatcga acgtcacacg
     1201 tggattcccg ccatcggcgc cgaatatctc cttggtatcg atggcctgag tctcctgctt
     1261 gtgttgctca cgtcggttat catccctttc gcatttctcg cccaacgcat gacgcgcgga
     1321 ttctgcgcaa tcatgctcat tctgcaatcg gcgctctatg gcacgtttac ggcgcaaaat
     1381 ttcgttctct ggtttttgtt ttacgagatg agcctgatcc ctgcgttcct gctcatcaaa
     1441 atctggggcg gccaaaaccg ggaccgggcg gcaacgaaat tcttccttta cacattcctc
     1501 ggcagcgtcg cgatgctgct ctcgtttctg ggaatttatt ttgcaacagg cacgttcgac
     1561 ttcgccggcc tcgccgatct cgggaaaaac ggattgctga ccggaaaggt cgcgtggttc
     1621 gccttcgctg gaatattcct cggactggcg gtgaaggtgc cgctgtttcc gtttcacacc
     1681 tggctgcctg atgcctacga aacagcgcca accggcgtgt cgatggtgct gacgggcgtg
     1741 ctctccaaga tgggcgttta cggtttcgtg cgcctgttgc ttccgctttt ccctaacgaa
     1801 atcaaaatcc tcggcccgtg gctgctggga ctggcggtgt gttcgattgt ctttgcgtcg
     1861 ctcgcggcat gggcgcaatc agatttgaaa cggatggttg cttatctgtc gatcaaccat
     1921 ctcggctatt gcatgctcgg tctctttgca gtgactgtct cgccaatcgt cgccgccagg
     1981 attgacacac aggcggcgtt gagcggtgcc ttcatgcaaa tcttcaacca cggcatcacc
     2041 gcggccgcgt tgttttatta cgtcggcttg cttgagcagc gccgcggctt gcgcggcatt
     2101 aatgatttcg gcggactaat gcaacgaacg ccgctgctct gcggatggat gagtgtcgcg
     2161 atgttttcgt cgcttggtct gccgggtctg aacggattca tcggcgaatt cttaatcttc
     2221 aaaggctcat tcgccatcgc cgcctcgttc accgcggtcg ccgtgatcgg cctgctcgtc
     2281 acggcaatcg ctttcgtacg cgcgatgcaa aagctcttca gcggcccgtt ggtcgaaagt
     2341 tgcagcgcct tccccgatct cctaccaact gagaaactcg tcgtcgttcc cgtgacgctg
     2401 ctcatgttcg ccatcggcat cgcgccgcag ttcgtcttca atatcttcaa cacaaccgtc
     2461 atccagatgg cgcgcttgtt tgcctgagtg ctgcgagaat gggcaaggaa atcattccga
     2521 ttgagcggat cgcgcgagcg attttcgtgt tccgcgggca gaaggtgatg ctggattcag
     2581 atttggcgac tttatatgga gtgaccacgg gaaacctaaa taaagctgtc aagcgcaacg
     2641 ctgatcgatt tccctccgat ttcatgtttc ggcttaagcc tgaggagctt gcaaacttga
     2701 aattccaatt tggaatatca agttggggcg gccgccggcg atcgcgtcct tatgttttca
     2761 cagagcaggg catcgccatg ttgtcgagtg ttctgaacag cga
//
LOCUS       DASQUQ010000180         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_39556, whole genome shotgun sequence.
ACCESSION   DASQUQ010000180 DASQUQ010000000
VERSION     DASQUQ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39556"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VE486_05135"
     CDS             <1..399
                     /locus_tag="VE486_05135"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZA38503.1"
                     /translation="EAAKVKALVNMVMNINTAGLAEGLALGAALGLDLEMLRKVFSQT
                     GAASRVLETDGEDMQNREHTCFFSAAHAAKDSGIALDLARDLGLELPLARSTKEQYDR
                     MIAEGLGELDKSGIAELTFKDRHKHSGDHV"
     gene            complement(613..807)
                     /locus_tag="VE486_05140"
     CDS             complement(613..807)
                     /locus_tag="VE486_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38504.1"
                     /translation="MKRRKVYRVAVAYIIAGGIIIQIAPAIFPAWDLPNWSLRLMVLV
                     LTGFPIAHLCVDLRYHAGWN"
     gene            992..1798
                     /locus_tag="VE486_05145"
     CDS             992..1798
                     /locus_tag="VE486_05145"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HZA38505.1"
                     /translation="MKENAQLKRDPVSTEPTCEIIAYQIEEDDVAPIRPAPRERKWIE
                     DAHEKFPYRCLPLVIANQYGWEVLSTHHVRAKWDGTSEPEGVTVEILYGDGEVHCHSH
                     FGEGVVTFQIPYIFRTPANWNLMVRGPTNSPKDGIVALDGIVETDWAHATFTMNWRFT
                     RAGTVEFALGEPICLFFPILRGALESFRGEFQMLESEPSLHTKFRGWSDSRDRFLSGL
                     DKEKPEIVAEGWQKDYLRAARAKKSMAHPFVDKRPAEAKQSDNHSRSNTL"
     gene            1811..2464
                     /locus_tag="VE486_05150"
     CDS             1811..2464
                     /locus_tag="VE486_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38506.1"
                     /translation="MKPHSRAYYARTARQFRRQRYLLVKGLLPQTMLEYLRVYYAILL
                     ANDEFATDEQCPRSLALGGDRALDAVLEWIRPEVSRLIGFALAPTYSYTRRYAKGERL
                     ERHCDREACEISVTVSLEIPKRAGPSEIYLKPPNSKERKIAMQEGDGCVYAGTEVEHW
                     RERFQTDGYVQLFIHFIARHGRHYPKCLFDGRESLGGNYELAARKKRRDIRRRRRQA"
     gene            complement(2620..>3028)
                     /locus_tag="VE486_05155"
     CDS             complement(2620..>3028)
                     /locus_tag="VE486_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38507.1"
                     /translation="AARESWLRARRELEPLLREQPENYSLIGDLALINMGLGEKTAAL
                     SLAERAIAANPVEQDAVRGPIPIEILARVAARFGEPDRAVAILEKLLSTPYDGAMAAS
                     APLTPALLRLDPMFDPLRNDPRFDKLTASPAAK"
BASE COUNT          725 a          833 c          888 g          582 t
ORIGIN      
        1 gaagcggcga aagtaaaagc gctcgtcaac atggtcatga acatcaatac cgccggcctg
       61 gctgagggac tcgctcttgg cgccgcactc ggcctggatc tggaaatgtt gagaaaagtc
      121 ttttcacaaa ccggcgccgc ctcgcgcgtg ctcgagactg acggcgaaga catgcaaaac
      181 cgcgagcaca cctgtttttt ctccgcggct catgccgcca aggacagcgg gatcgcgctc
      241 gatctcgcgc gcgatctcgg tcttgaatta ccactcgccc gctccaccaa ggagcaatac
      301 gaccgcatga ttgcggaagg cctcggtgag ctcgacaaat ccggcatcgc cgagctcacc
      361 ttcaaagatc gccataagca ttccggcgat cacgtgtgaa cgacttctgc tcgcgctgcc
      421 gcgccgtagc gcgttggccc gcaggcgggc aatagtgccc gggcgaatcg gccacagggc
      481 cgtttctggg cacccgcgca caatcgagtc gaggcaaatc acagacgggg cggtcatctt
      541 gcgggtcaca acctggcggt gacaacaatc cgggacgacg atgcgaatct gggcgcggtt
      601 atcgaagcgc gcttaattcc acccggcgtg atatcgaagg tccacgcaaa gatgagcgat
      661 cgggaaaccg gtcaggacga ggaccatcaa acgcaaactc cagtttggca ggtcccaagc
      721 cggaaaaatc gccggggcga tctgaatgat gataccgccc gcgatgatgt aggcgacggc
      781 cacgcggtaa acttttcggc gcttcaatcg tcaaagaagc cgctcataat aaaagattaa
      841 ccgggtgcga aggaaacgcg agcatgcact cactcagaca acaataaaac cgacatgttg
      901 aatgctgttt cgctttacac actaattgtc atttgctaag gctgccaccg gacagcgata
      961 ccactgcgtc ccaattagcg attaccatga catgaaggaa aacgcgcagc ttaaacggga
     1021 tccagtttca acggaaccaa cgtgtgaaat cattgcctat caaattgagg aagatgatgt
     1081 cgcgccgatt cgaccggcgc cgcgggaacg gaaatggatc gaagacgccc acgagaagtt
     1141 tccttatcgg tgtctgccgc tggtgatcgc aaaccaatac ggctgggaag ttctatcgac
     1201 acatcatgtg cgtgcgaaat gggacggaac ctccgaacca gagggtgtga cggtggaaat
     1261 cctttatggt gacggtgagg tgcactgcca ttcacacttt ggtgagggcg tcgtaacttt
     1321 tcagattcct tacatattca gaacaccggc gaactggaat ctcatggtac ggggtccgac
     1381 gaatagtccg aaggacggta ttgttgcgct ggatggaatt gtcgagaccg actgggcgca
     1441 cgcgactttc acaatgaact ggcgtttcac tcgggcaggc acggtcgagt tcgcgctcgg
     1501 tgagccaatc tgcttgttct ttcccatcct acgtggagcg ctagagagtt ttcgcggcga
     1561 gttccagatg ctggagtccg agccgtcgct gcacacaaag tttcgaggtt ggtctgatag
     1621 ccgcgatcgt tttttgtcag gcctggataa ggagaaaccg gaaatcgtgg ccgaggggtg
     1681 gcagaaggat tacttgcgcg cggcgagagc gaaaaaatcg atggctcacc cctttgttga
     1741 taaaaggccg gccgaagcaa aacaatccga caatcattcg cgttcgaaca cgttgtaggc
     1801 cctcgttagg atgaagcctc attcccgcgc ttactacgcg cgaacagcac gacagttccg
     1861 gcgtcaacga tatctgttgg tcaaaggttt gctgccccag acaatgctgg aatacctgag
     1921 agtctattac gcgatcctgc tcgccaatga cgagttcgcc accgacgaac aatgcccccg
     1981 gtcgttggcg ctcggcggtg accgcgcgct cgatgccgtg ctggaatgga tccggccgga
     2041 agtgagccgg ctgatcggat tcgcgttggc cccaacctat tcctacacac gaaggtacgc
     2101 caaaggagag cgcctggagc ggcattgcga tcgtgaagcg tgcgaaatct cggtcacggt
     2161 ctcgctcgaa attccgaaac gggccggacc ttccgagatt tatctgaagc cgccaaattc
     2221 gaaggaacga aaaatcgcga tgcaggaagg cgatggctgt gtttacgccg gtaccgaagt
     2281 ggagcactgg cgcgagcgct tccaaaccga cggctatgtt caacttttta tccatttcat
     2341 cgcgagacac gggcgtcact atccgaagtg cctttttgat ggacgtgaat ctctcggcgg
     2401 taactacgaa ttggcggcac ggaaaaaaag gcgtgacatc cgtcgccgga ggaggcaagc
     2461 ttgagcctaa cgtatgggaa atgtttctcg caaaaaatcc agcgggcgca aatcagccag
     2521 acggaaagca aaaggtcccg ggccgaacgt gcgcaaacgc aaaaagcgac ggtagagacc
     2581 tagcaagtcc tgcgaccagg cagtgttggg ccgcgcaaat cactttgcgg ccggcgaagc
     2641 cgtcaatttg tcgaagcgcg gatcgtttcg gagcggatcg aacatcggat cgaggcggag
     2701 caacgccggc gtgagcggcg cggacgccgc catcgcccca tcgtagggtg tagaaagaag
     2761 tttctctaga atagcaacgg cgcgatcggg ttctccgaag cgggccgcga cgcgggccag
     2821 gatctcgatc gggatgggac ctcgaactgc gtcctgctca accgggttag ccgcaatcgc
     2881 gcgttcggcc aaactcagag cagccgtctt ctcgccgagg cccatgttaa tcagcgcaag
     2941 atcgccaata agactgtaat tttccggttg ttcgcgcagc aaaggctcga gttcgcggcg
     3001 cgcgcgaagc caactttcac gcgcggca
//
LOCUS       DASQUQ010000181         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1206815, whole genome shotgun sequence.
ACCESSION   DASQUQ010000181 DASQUQ010000000
VERSION     DASQUQ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206815"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..1341)
                     /locus_tag="VE486_05160"
     CDS             complement(352..1341)
                     /locus_tag="VE486_05160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZA38508.1"
                     /translation="MRTELLLRQTRMHFPRLDVAEIKIAPINKGGSDRKFYRIRCSPE
                     QTLILVKYNLEREENRQYVRIAKFLDTHGIRVPKIYFHDATEGLIWIEDLGERDLFSY
                     RKESWLVRRAFYESALDQIAALHGLSESVCIEIKEDLPAEFNAELYVWEQNYFFENCL
                     GRYFAIDSEKLASLAALPALNQIAEHLDRLPRVLVHRDFQSQNIIMRNGQAHLIDFQG
                     MRPGLAEYDLASLVYDPYVDLTEADRAELIAYYREQQIKNGMTINSDFDSTLRLCAMQ
                     RLMQALGAYGFLGLVKGHKHFLKHIPAAMKSLRFIVTRTAGLTDLEKVLAACT"
     gene            complement(1357..>2140)
                     /locus_tag="VE486_05165"
     CDS             complement(1357..>2140)
                     /locus_tag="VE486_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HZA38509.1"
                     /translation="RHSVTLVHEPELLETGGGIRNAERYLGSEPFITYSGDVLTDINL
                     RPLIDEHFRRGNDVTLSLRETGLAAAVALRDHRVVDISNRYGVAGNYDFANIAIWNRQ
                     IFQRIPPHRKISFVPIIGDWIGQGGKIGGLVMNDGKWFNIGSRAEYLEVHRELSMKGD
                     SPIAPARPKPPLPGSALNFPAYPVNDPGWRMPVHKTAIVDPSAQLRGCTVIGRDCRVG
                     AEAILEDTILWPGAEIASKSHLEGCIVRSEKKASGSHRNIDI"
BASE COUNT          468 a          610 c          585 g          477 t
ORIGIN      
        1 gcaggcactt ccctgagtga tcaggtcacg ttcccggcca acgctggcga cacggtgcaa
       61 ctggttgtcg gcggcgcggt gctcagcctg ggcccaggtg acaacgcgac catcaacatc
      121 gacaaagtgc aataagaaca cctgaaacct ctgtaccatc cttagacgct cgcgcagatt
      181 tattcggggc tcgctggcgg tggccggtcc ggtgagggcg atctccgcgc cgagctttcg
      241 gtgatttgtg ccaagagagc cccgcttcca gcgggcctcg gtggcattct gcgaaacagg
      301 tctccgtcgc aagtgtgcgg cggactgtcg gcaggattcc ggtgctcccg atcaagtgca
      361 cgcagccagc actttctcga gatcggtcaa gccggcagtt cgcgttacaa tgaaacgtaa
      421 tgatttcatt gcagcaggaa tgtgcttcag aaaatgtttg tggcctttca cgaggccgag
      481 aaaaccgtac gccccaagtg cctgcattag acgctgcatc gcgcaaagcc gcaacgtgga
      541 atcgaagtca ctgttaatcg tcattccgtt tttgatttgt tgctcgcgat agtacgcgat
      601 caattccgcg cgatccgcct ctgtgaggtc gacatacgga tcgtacacca gcgaagccag
      661 atcgtattcc gcgaggccgg gccgcatccc ctgaaaatcg atcagatgcg cctgcccgtt
      721 tcgcataatg atgttttgcg actgaaaatc gcgatgcacg agaacgcgtg gcaaccgatc
      781 gagatgctcg gcgatttgat tcaacgccgg caaggcggca agtgaagcaa gcttttcgct
      841 gtcgatggca aaatagcggc cgagacagtt ttcgaaaaaa tagttctgtt cccaaacgta
      901 aagctccgcg ttgaactccg ccggcaaatc ctccttgatt tcgatgcaga ccgactccga
      961 caagccgtgg agcgccgcaa tttgatcgag ggccgactcg taaaacgcgc ggcgcaccag
     1021 ccagctttcc ttgcggtaac tgaaaaggtc ccgctcgccc aggtcctcaa tccagatcaa
     1081 accttccgtc gcatcgtgaa aataaatttt cggcactcga attccgtgcg tatcgagaaa
     1141 tttcgcgatt cggacatact gccgattttc ttcccgctcc agattgtact taaccaggat
     1201 gagggtctgc tccggcgagc aacggattcg ataaaatttt cggtccgatc ctcccttgtt
     1261 aatcggcgcg atttttatct cggcgacatc cagacgcggg aaatgcatcc gcgtttgccg
     1321 aagaaggagc tcagttctca ttactctggg ggattcttag atgtcgatgt tgcgatggct
     1381 gccgctggct tttttttcgg agcggacaat acaaccctcg aggtgacttt tggaagcaat
     1441 ttctgctccg ggccacagaa tcgtgtcttc caggatcgcc tccgctccaa ctcgacaatc
     1501 tcgcccgatg actgtgcagc cacgcaattg cgcgcttgga tcgacaattg ctgttttgtg
     1561 gaccggcatt cgccagccgg gatcgttcac cggataagcc gggaaattca gcgcgctgcc
     1621 agggagcggc ggtttcggtc gcgccggggc gatcggggaa tcgcccttca ttgataattc
     1681 gcgatgaact tccagatatt cagcgcgcga cccaatattg aaccactttc catcgttcat
     1741 caccaagccg ccgatcttgc cgccctggcc gatccaatca ccgataatcg gaacgaaaga
     1801 gattttccgg tgcggcggga ttcgctggaa aatctgccga ttccaaatgg cgatattggc
     1861 aaaatcgtaa ttgccagcca cgccgtacct gttcgatatg tccacaacgc ggtggtcgcg
     1921 caaagccacg gccgcggcca aaccggtttc gcgcaacgag agcgtgacgt cattcccgcg
     1981 acgaaaatgt tcgtcgatca aaggtcgcag attgatgtcg gtcaggacgt cgccgctgta
     2041 agtgatgaat ggctcgctgc cgagatagcg ttctgcattc ctaatgccgc cgccggtttc
     2101 aagcagctcc ggctcgtgaa ccagcgtgac cgaatggcgg
//
LOCUS       DASQUQ010000182         3670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1127892, whole genome shotgun sequence.
ACCESSION   DASQUQ010000182 DASQUQ010000000
VERSION     DASQUQ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127892"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VE486_05170"
     CDS             <1..410
                     /locus_tag="VE486_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZA38510.1"
                     /translation="PQTFTGPMVALMDEFSASDGDIFPYRFKTLGLGKLIGKRTWGGV
                     VGIRDPLPLTDGGNLFKPEFAPYSKEGKDWIIEGQGVDPDIFVDNDPAKEFKGEDQQL
                     DRAIQEIQDDLKTKRYDLPPIPPYPNRNPVKGS"
     gene            737..1174
                     /locus_tag="VE486_05175"
     CDS             737..1174
                     /locus_tag="VE486_05175"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HZA38511.1"
                     /translation="MSEQGLETIESTTQKTHEWIASIAETLHMEKRDAYKALRAVLQT
                     VRDRLPVDVAVHFGAQLPMLVRGLYYEGWEPSKAPIKMSRKEFLDAIVEKIVAGRVIN
                     PAETTESVLAVIASHVGTGEMQKLMHSFPHDMQALFPPLASAA"
     gene            1194..1877
                     /locus_tag="VE486_05180"
     CDS             1194..1877
                     /locus_tag="VE486_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HZA38512.1"
                     /translation="MERAFPNRTEAGRMLAEKLINYAGHNDVIVLGLPRGGVPVAYEV
                     AKRLSAPLDVFIVRKLGVPGFEELAVGAIASGGVRVLNKDVFRALPNAEETIELITAT
                     ETAELERREQEYRDGRPAPELRDRVVILVDDGLATGATMRAAVKALRQRDVAKIVVAV
                     PVGPPDTCREVEDEADETVCLSMPDFFQAVGQYYEDFSQTSDEEVRELLARASQDRSS
                     EGQIRGGEQ"
     gene            1874..3199
                     /locus_tag="VE486_05185"
     CDS             1874..3199
                     /locus_tag="VE486_05185"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HZA38513.1"
                     /translation="MIDAELFREIAQPLTGKANDYDSLLDLIGDSRVVLLGEASHGTH
                     EFYFERAAITKRLIAEKGFTVLAIEADWPDASRVHRYVRGISDDANANEALSGFRRFP
                     VWMWRNTVVVEFVEWLRKFNKDIDPKRAPAGFYGMDLYSLHASIDAVLSYLQKVDPDA
                     AKRARLRYSCFDHFSREPQEYGYAATVDATESCEDVVVEQLAELQRKAGEFLSRDGEV
                     AAEELFFAEQNARLVKNAEQYYRSMFRGRASSWNLRDRHMVETIEALVAHLDGSRQPK
                     AIIWAHNSHLGDARATEMSQHGELNVGQLIRERFGKESVLIGFSTHHGTVTAASDWGE
                     RAERKNVRPALEGSYEELFHQTGLSRFWIALRNVGDKVGVLSEPRLERAIGVIYRPET
                     ERLSHYFHARLPKQFDAIIHIDETRAVEPLERTSIWDKGELPETYPFKV"
     gene            3306..>3670
                     /locus_tag="VE486_05190"
     CDS             3306..>3670
                     /locus_tag="VE486_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38514.1"
                     /translation="MNEVQIQAGRAVLSGNLHIPEGAAALVLFAHGSGSSRHSPRNQS
                     VARTLNDAGLATLLFDLLTQEEEAIDMQTREHRFNIGLLAERLVHATRWAKQQDETRD
                     LQIGYFGSSTGGGAALVAAA"
BASE COUNT          851 a          975 c         1083 g          761 t
ORIGIN      
        1 cgccgcagac tttcaccggc ccaatggtgg cgctgatgga tgagttctcc gcgtccgatg
       61 gcgacatttt tccataccga ttcaaaacac ttggcctggg taaattgatc ggcaaacgca
      121 cctggggcgg tgtcgttggt attcgcgatc cgctcccgct caccgatggc ggcaaccttt
      181 tcaagcccga gttcgcccct tattcaaaag agggcaagga ctggatcatt gaaggacaag
      241 gtgtcgatcc agacatcttc gtggacaacg atcccgcaaa ggaattcaaa ggcgaagatc
      301 agcagctcga ccgggcgatc caggagatcc aagatgacct gaagaccaaa cgctatgacc
      361 tgcctccgat cccaccctac ccaaacagga acccggtaaa gggtagctag agaccgtttg
      421 gctcattgag aaggccgcgg cgcaaaatga cgaagcacga atgtcgaatg acgaaagaag
      481 cccgaataat ctaatgactt agcgctcgct gcggcgagag ccactttatt tgttcgacat
      541 tcggatttcg tcattccttc gcggttcgtc attcgacatt cgtcatttga gcgtcgggtg
      601 cgccgattgg tcgcgccgaa caagaccgac gacccaccgt tggatttgag aacacgcgac
      661 ccggcgtcga gaagcgaaat cactaaggag gcattttgtc tcggagaagc atttaaggaa
      721 cgggaggatc acagatatga gcgagcaagg tcttgaaacc attgaatcaa caacgcagaa
      781 aacacacgag tggattgcga gcatcgcgga aacattgcac atggaaaaac gggacgctta
      841 caaggcgctc cgggcagttt tgcagactgt gcgtgaccgc cttccggtcg atgttgcggt
      901 tcatttcgga gcgcaactgc caatgctggt gcgcggcctg tactatgaag gatgggaacc
      961 gtccaaggcg ccgatcaaga tgtctcgcaa ggaatttctt gatgcgatcg tggaaaagat
     1021 cgtggctggc cgcgtgatta accccgcgga aacaacggaa agtgtcctgg ccgtgatcgc
     1081 gagccatgtg ggcacgggcg agatgcaaaa gctaatgcac agttttccgc acgatatgca
     1141 ggcgttgttc cccccgcttg cctccgccgc ttaaaacgtg gcgggcagcc cgcatggaaa
     1201 gagcttttcc caaccgaacc gaagcggggc gcatgctcgc agagaagctg atcaactatg
     1261 ctggccacaa tgacgtaatt gttctcggct tgccgcgcgg cggcgtgccg gtcgcctacg
     1321 aagtggccaa acggctgagc gcgccccttg atgttttcat tgtgcgcaaa ctcggcgtgc
     1381 ccggtttcga ggaactcgca gttggcgcaa tcgcttccgg cggggtgcgc gttttgaata
     1441 aggatgtgtt tcgcgccttg cccaatgccg aggaaaccat cgaattgatc actgcgacgg
     1501 agacggccga gctggaacgc cgcgaacaag aatatcgcga tggccggcca gcgccggaat
     1561 tgcgcgatcg tgttgtcatt ctggtcgatg acggcctggc caccggcgcg accatgcgcg
     1621 ctgcggtgaa agctttgcga cagcgtgacg ttgcaaaaat tgttgtcgcg gtcccggtcg
     1681 gtccgccgga tacatgtcgt gaagttgaag acgaagcgga cgagaccgtg tgcctgagca
     1741 tgccggattt ttttcaggca gtcggccagt actacgaaga tttctcgcag acgagcgatg
     1801 aagaagtgcg cgagctgctc gcccgcgctt cgcaggaccg atcctccgaa ggacagattc
     1861 gcggtggcga acaatgatcg acgccgaact cttccgcgaa attgcccagc cgctaaccgg
     1921 caaagcaaac gattatgatt cgctgctcga tctgattggc gattcgcggg tggtactact
     1981 gggcgaagct tcgcacggca cgcatgaatt ttatttcgag cgcgcggcga ttacaaaaag
     2041 gctaatcgct gaaaaaggtt tcacagtgct cgcgatcgag gcggactggc cggacgcatc
     2101 tcgcgtccat cggtacgtgc gcggcataag tgatgatgcg aatgcaaacg aagcactctc
     2161 tggcttccgc cgttttcctg tctggatgtg gcgcaacacc gttgtcgtcg agttcgtcga
     2221 gtggcttcgc aagttcaaca aagatatcga tcccaaaagg gcgcccgcgg gtttttacgg
     2281 aatggatctt tacagtctgc acgcttcaat cgacgcggtt ctaagttacc tgcaaaaggt
     2341 tgatcccgac gcagcgaaac gcgcgcgttt gcgttattcg tgtttcgatc atttcagccg
     2401 cgagccccaa gagtacggtt atgccgcgac ggtggacgcc acggaatcgt gcgaagacgt
     2461 agtcgtcgag cagctcgccg agttgcagcg caaagcgggc gaattcctca gccgcgacgg
     2521 tgaagtggcc gccgaggaac ttttcttcgc tgagcaaaac gcgcggctgg tgaaaaacgc
     2581 ggaacagtat taccgatcca tgtttcgcgg tcgcgcatca tcctggaatt tgcgcgaccg
     2641 gcacatggtg gaaacgatcg aggcactggt cgcgcacctc gacggcagcc ggcaaccaaa
     2701 agcgattatt tgggcacaca attcccatct gggcgacgcg cgcgcgaccg agatgagcca
     2761 gcacggcgaa ctgaatgttg gtcagctcat tcgcgagcgc ttcggaaaag aatcggtgtt
     2821 gatcggtttc agcacgcatc acggcactgt aaccgcggcc tcagattggg gcgagcgtgc
     2881 cgagcgcaaa aatgtgcggc cagcactgga aggcagttat gaagagctgt ttcatcaaac
     2941 cgggctgtcg cgattttgga ttgccctgcg aaacgtaggc gacaaggtcg gcgtcttgtc
     3001 cgagccgcgg ctggagcggg cgattggtgt gatctaccgg cccgagacag aacggttgag
     3061 ccattatttt catgcgcggc tgccaaagca attcgatgcg atcattcaca tcgacgaaac
     3121 acgcgcggta gagccgctcg aacgaacgag tatttgggac aagggtgagt tgcccgagac
     3181 atatccattc aaagtttgat ctgcgcttta atcgtaatct tattcctatt cttaatctta
     3241 ctcttgttcg catgccagca tttgagatta ggaatgcgaa taagattaag aataggagct
     3301 aaatcatgaa cgaagtgcaa atccaggccg gacgtgcagt tctctcggga aatttgcata
     3361 ttcctgaagg cgcggccgcg ctcgttcttt ttgcccatgg tagcgggagc agccggcaca
     3421 gtccgcgcaa tcaatccgtt gcgcgcactt tgaatgatgc cgggctagcg accctgcttt
     3481 ttgatctgct cacgcaggaa gaggaagcca tcgacatgca aactcgcgag catcgattca
     3541 acatcggttt gctggctgaa cgactggttc acgccacaag gtgggcaaag caacaggatg
     3601 aaacgcgcga cttgcagatc ggttatttcg gctcgagcac cggaggcgga gcggcgttgg
     3661 tcgcggcagc
//
LOCUS       DASQUQ010000183        10185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_319399, whole genome shotgun sequence.
ACCESSION   DASQUQ010000183 DASQUQ010000000
VERSION     DASQUQ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319399"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..2931
                     /gene="clpB"
                     /locus_tag="VE486_05195"
     CDS             358..2931
                     /gene="clpB"
                     /locus_tag="VE486_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZA38515.1"
                     /translation="MRIDKFTQKMQEALQAAQELASQSNHQEVTNEHFLSALLDQADG
                     ITRPLLEKIGVPVARLREGLASELERRPKVHGAAVDLRLSNELRSVLDGAEKEMSKLK
                     DEYTSAEHYLLALSAANAPAAKILKNLGVTRDKLMQALQQVRGSQRVTDQNPEGKYQA
                     LEKYGRDLTELARRGKIDPVIGRDNEIRRIMQVLSRRTKNNPVLIGDPGVGKTAIVEG
                     LARRIISGDVPDSLKNKRIIAMDIGAMVAGAKFRGEFEDRLKAFLKEVTDSQGQVILF
                     IDELHTIVGAGAAEGSVDASNMLKPMLARGELHTIGATTLDEYRKYIEKDAALERRFQ
                     PVLVEEPSVEDTIAILRGLKERYEVHHGVRITDAALVAAAVLSHRYISDRFLPDKAID
                     LVDEAASRLKIELDSMPTEIDVIEREIMQHEMERQALKKEKDPASKERLKKLEKELAE
                     LKEKSSALKAEWQKEKGVIEEQRKWKEELDQLRTELERAQRRGELAKASEIQYGRIPE
                     LEKKLADATARTAKDRKSTLLREEVTEEDIAEVVSNWTHIPVSRLQEGEREKLVKLEE
                     HLHLRVVGQDEAIKAVSNAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELSRALAEFL
                     FDDENAMIRIDMSEYQEKHTVARLIGAPPGYVGYEEGGQLSEAVRRKPYSVVLFDEIE
                     KAHPDVFNVLLQVLDDGRITDGQGRTVDFKNTVIIMTSNIGSQYITDEESKEARRRLV
                     MDALRAHFRPEFLNRVDEIIIFDRLTEDDLKKIVEIQLRRLSKRLEQQKITLKLSNSA
                     KELLAREGYDPVYGARPLRRTIQKEILDRLSIDILEGKVREGQTVYVNAKDGALEFQE
                     K"
     gene            2974..3318
                     /locus_tag="VE486_05200"
     CDS             2974..3318
                     /locus_tag="VE486_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38516.1"
                     /translation="MSARSKPKIQFNGGEYASAEEMPVSIRAAYDRAVGDIPVLHSGA
                     RLAAKLNAKIILNETEFNNPGEMSVDDRHLYHEALAALFPASIAVSVSEDRERPRWRC
                     LSLIARLLRLKR"
     gene            3420..4685
                     /gene="hutI"
                     /locus_tag="VE486_05205"
     CDS             3420..4685
                     /gene="hutI"
                     /locus_tag="VE486_05205"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZA38517.1"
                     /translation="MSSLVVLHASQLITLAGPKRARVGSQLSELAVVHDGGMLVHDGV
                     IVAIGSSAEIEKNAGDSDVIDARGRVVLPGFIDAHAHPLFAGNRLADFERRARGETYE
                     QIAAGGGGIWSTVEKTRAATETELLNQAKRHANWFLKCGTTTVEAKSGYGLTLEDELK
                     ILRVIRRLNEETQIEFVPTFLGAHAIPRECRADQYVELVINDMLPRVVSEKLAEFCDV
                     FCERGYFDIEQSRKILAAAKKLGLKLRIHADQLTNSGAAKLAAELEATTADHLEKTDG
                     KGIAAMKSAGVQPVLLPGSVYALGSTDYPCAREMIEAGLAVVIATDFNPGSSPSPSMP
                     MMLSLACTQMKMSPAEAITASTINAAYSLNRGDKIGSLEQGKLANFSIFDCEDYREVA
                     YYFGFPQTHSVYVRGRRVCQALFKSGGGT"
     gene            4682..5050
                     /locus_tag="VE486_05210"
     CDS             4682..5050
                     /locus_tag="VE486_05210"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZA38518.1"
                     /translation="MKEELLKLEKEFTQAIVKNDADAITRFLADDWIIIDADGGIVDK
                     ARFLGVIKSGALTHEMMESDEVAVRIYGEAAVVTGLTATKGKFMGQEFSTRERATDVF
                     VKQSGRWRCVFSQLTRFSKK"
     gene            5222..6559
                     /gene="tig"
                     /locus_tag="VE486_05215"
     CDS             5222..6559
                     /gene="tig"
                     /locus_tag="VE486_05215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZA38519.1"
                     /translation="MKVEVETQPGSVSTLQIELPPEEVSKEWDSIANSFARLAKIPGY
                     RPGKAPRTVIEKRFRKQIEEELTKKLVSKSYHEAIEQQQLRVASLANIEDVQLGEDKS
                     MRFRATVVTAPEFDLPEYKNIPIQLPDTKVSEADIDTALERVRDQAADFVDVPERELQ
                     MGDFAVIDFEGSIDGKPIAEIAPQASRNLHGGKKFWLHLAPENFLPTFCEKMVGQKRG
                     EKRQITIVFPADFPVKELATKEANYAVTLQEIREKVLPALDDALANKLIPGKTLADLR
                     HLIEHDLEHEKEHEVERAKEAQILKYLHEHISFELPPALMKNETRRVLGELVQRNRER
                     GVSDEMLKEKEKELIETAAGVAVHRLKTNFILHRIAERENIKTSRQEIDARIRGDAAR
                     YNISPEKMRKELEEHDALDSVAEQILLGKTLDFLKANVSVERIQEPPVVEEKS"
     gene            6556..7260
                     /locus_tag="VE486_05220"
     CDS             6556..7260
                     /locus_tag="VE486_05220"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZA38520.1"
                     /translation="MSASNENRSQSVLVPMVVEQTGRGERSYDIYSRLLKDRIVFIGT
                     PMDDHIANLVIAQLLFLQMEDSKKDINIYINSPGGSVTAGLAVYDTMQFLTCDVTTYC
                     LGMAASMAAVLLCAGTKGKRFALPNSDIMIHQVSGGAQGAASDVERQVDYMFKLKKRL
                     IKIIAQHTSKSEEQVRLDSDRDYYMSAQEAKEYGLVDEVIKSRKEVKLLDGATPPSGD
                     RSVAVAEAALPRKAGG"
     gene            7284..8564
                     /gene="clpX"
                     /locus_tag="VE486_05225"
     CDS             7284..8564
                     /gene="clpX"
                     /locus_tag="VE486_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZA38521.1"
                     /translation="MARASNLTMCSFCGKSHAEVRKLIAGPGVYICDSCINVCKSILD
                     KELNEDARRQSSNIRVPKPAEIRRLLDQYVIGQDTAKKTLSVAVHNHYKRVLQPSAPG
                     DASAAFQPDPFADVEIEKSNILLIGPTGSGKTLLAKTLARILDVPFCIADATTLTEAG
                     YVGEDVENIILRLLQNADYDVKRAEIGIVYIDEIDKIGRKTDNVSITRDVSGEGVQQA
                     LLKILEGSTCNVPPQGGRKHPHQEYIQVNTEKILFICGGAFIGLDKIIQKRIGQKVMG
                     FGSPTLEVEETLRKQAIRHVEPEDLLAFGMIPEFIGRLPVVSSLNALTEEEMVAILTE
                     TKNAMVKQYTKLFSMEGVRLTLTKDALRALAAQAVTKGTGARALRSMLERIMLDIMYD
                     VPSREDIAEVTINRAVVEGRKLPLIRKKQDKDAA"
     gene            8701..9936
                     /locus_tag="VE486_05230"
     CDS             8701..9936
                     /locus_tag="VE486_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HZA38522.1"
                     /translation="MRVVVLNTGTELLLGDVQDAHLAFIAREILPLGLRINERRTVPD
                     GPAIRDGLSELFGRSEILFVTGGLGPTSDDITREIVADLLRLELRQDAQLLASLKERL
                     AARGIKWTSRIARQAEVPAGAQVLPNENGSASGLYLKTNINPLIPSPHVFVLPGPPRE
                     LQPMFRESVTSILRSIVQVPASIERRLYKIACLGESTIEQAIGDKVLAIPGIELGYCA
                     RPGEVDIRIVGETDVVSRADVIIKKELGSSIFSANDEALEEVLVNLLSSRKETLAIAE
                     SCTGGLLAHRITNIPGASSVFLAGYICYANEAKIDMLEVDPGLIEKHGAVSEPVARAL
                     AQHARMHARSTYALATTGIAGPSGGSPEKPVGTIYIALATPAEIAVKKLFFPSDRETF
                     KQMAAQAAFDLLRRKVIET"
BASE COUNT         2469 a         2674 c         2944 g         2098 t
ORIGIN      
        1 gtaccactgt cggaaatgag cgagccaatg atatagcggc gaaaaggacc ctgacgcaaa
       61 agctccagcg actttgctcc agtccatttt tccttctcgt tcatcgccaa gactcatcgt
      121 tgtagtggcg tttgtgtcaa acaccgtggc gctgaagctg caacgggtag cgcacgcgtc
      181 tcgcgtggcg gcgaccgcgt cctcgcggtc gcggacttcc cctttgactt cgggttcttc
      241 gctgcggacg accttcgagg aaagattgtt tcggcgcgac gccgaaacca gcacgcggga
      301 cgcgtgcgct acccaaacga cgaattgccg atgtagtcgc gttcactatc ttgaagcatg
      361 cgcatcgaca agttcaccca gaaaatgcag gaagccctgc aagcagcgca ggaacttgct
      421 tcacaatcca atcaccagga agtcacgaac gagcattttc tttccgcgct tctcgatcaa
      481 gcggatggaa taacgcggcc gctgctggaa aagattggtg tgccggtggc tcgtttgcgc
      541 gaaggcttgg ccagcgaact tgagcggcgt ccgaaagttc acggcgcagc cgtcgatctg
      601 cgtctttcga acgaactccg cagcgtcttg gacggcgcgg agaaggaaat gtcgaagttg
      661 aaagacgaat ataccagcgc cgagcattat ctgctcgcgc taagcgccgc gaacgctccc
      721 gccgcgaaga tcctgaagaa tctcggcgtc acgcgcgaca agttgatgca ggcgctgcaa
      781 caagtgcgag gttcgcagcg cgtgaccgat caaaatcccg aaggaaaata tcaagcgctc
      841 gaaaaatacg gtcgcgacct gaccgagctg gcgcgacgtg gaaagattga tcccgtaatc
      901 ggacgcgaca acgaaatccg acgcataatg caagtgctct cgcgtcggac gaaaaataac
      961 ccggtgctta tcggcgatcc aggcgttggc aaaaccgcaa tcgtagaagg cctggcgcgg
     1021 cgcatcatca gcggcgacgt gcccgattcg ctgaagaaca aacggatcat cgcgatggac
     1081 attggggcga tggtcgcggg cgcgaagttc cgcggcgaat ttgaggatcg tctgaaagcg
     1141 ttcttgaagg aagtgaccga ttctcagggg caggtcattt tgttcatcga cgagctgcac
     1201 accattgtgg gcgctggtgc ggcggaaggt tccgtcgatg cgtccaacat gcttaagccg
     1261 atgctcgcgc gcggcgagtt gcacacgatc ggcgcgacca cgctcgatga atatcgcaag
     1321 tacatcgaga aagacgctgc gctcgagcgg cgtttccagc cagtactggt ggaggagccg
     1381 agcgtagaag atacgatcgc gattttgcgc gggctgaagg agcgctacga ggtgcaccac
     1441 ggcgtccgca tcaccgacgc ggcgctcgtg gccgcagcgg tgctttcgca tcgctacatc
     1501 agcgatcgtt ttctgccaga caaagcgatc gatcttgtcg atgaggcagc ctcgcgtctg
     1561 aagatcgagc tcgactccat gccaaccgag attgacgtga tcgaacgcga aatcatgcag
     1621 cacgaaatgg agcggcaggc gctgaaaaag gaaaaagatc ccgcaagtaa agagcgcttg
     1681 aaaaagcttg agaaagaatt ggctgagctg aaggaaaaat cctccgcgtt gaaagccgag
     1741 tggcaaaagg aaaaaggcgt catcgaggag cagcggaaat ggaaggaaga actcgatcaa
     1801 ctgcgcaccg agttggagcg cgcccagcgc cggggcgaac tggcaaaagc gagcgaaatt
     1861 caatacgggc gtattccaga gctggagaag aaattagccg atgccacggc aaggacagcg
     1921 aaggatcgaa aatcaacgtt actccgcgaa gaagtgaccg aggaagacat tgccgaagtc
     1981 gtttcgaact ggacgcacat tccggtctcg cggttgcagg agggcgaacg ggaaaaactg
     2041 gtcaagctgg aggagcattt gcatttgcgc gttgttgggc aggacgaggc gatcaaagcc
     2101 gtttctaacg cagtgcgccg cgcgcgagcg ggcttgcaag atccgaaccg tcccctgggc
     2161 tcatttcttt ttcttgggcc gactggcgtc ggcaagaccg aattatcacg agcactggca
     2221 gaatttttgt tcgacgacga aaacgccatg atccggatcg acatgagcga ataccaggag
     2281 aagcacacgg tcgcgcgatt gattggcgcg cctcccggat acgtcggtta cgaggaaggc
     2341 gggcaacttt ccgaagccgt ccgacgcaag ccttattcag tcgtgctctt cgacgaaatc
     2401 gaaaaggcgc atccagacgt attcaacgtc ttgctgcaag tgctcgacga cggccggatc
     2461 accgatggac aggggagaac tgtcgatttc aagaacacgg tcatcattat gacgtcaaac
     2521 atcggttcgc aatacatcac tgacgaagaa tcgaaggaag cccgccgtcg actcgtcatg
     2581 gatgcattgc gcgcccattt ccgtccagag tttctcaatc gcgtcgatga gatcatcatt
     2641 ttcgaccgac tgactgagga tgatttgaag aagattgtcg aaattcagct tcgccgcctg
     2701 tccaaacggc tcgagcaaca aaagatcacg ctcaagctct ccaattcagc caaggaactg
     2761 ctggcgcgcg aaggatacga tccggtttac ggcgcgcgtc cgctgcggcg caccattcag
     2821 aaagaaattc tcgatcggct cagcatcgat atcctggaag gcaaagtccg cgagggccaa
     2881 accgtctacg tcaatgcaaa agacggcgcg ctagaatttc aggaaaaata gttgtgaaag
     2941 tatgggattg gaggtttggt tctcgtcttt ttagtgagcg cgaggtccaa acccaagatc
     3001 cagttcaacg gcggcgagta cgccagtgcc gaggagatgc cggtcagcat tagggcggcg
     3061 tatgatcgcg cggttggcga tatccccgtg cttcattccg gggcgcgact ggctgcaaaa
     3121 ctgaacgcca agatcatcct gaacgagacc gagttcaata accccggcga aatgtcggtg
     3181 gacgatcgac acttgtatca cgaagcgctc gcggccctgt tcccggcgag cattgcggta
     3241 tccgttagtg aggatcgtga gcgtcctcgc tggaggtgtt tatctctgat tgcacggctt
     3301 cttcggctaa aacgctgaac gcgtttcaat tttctggaac atagatcttg ggtgaagcgt
     3361 tgaagggttg aagcggcgac gcaaatccgc tttaacgttt ttaacgcttc aacgacatca
     3421 tgagttcact ggtcgttctt cacgcctcac aacttatcac gctggcggga ccgaaacggg
     3481 cgcgtgttgg atcacaattg tcggagctgg cggtcgtaca tgatggggga atgctggttc
     3541 atgatggagt catcgtggcg attgggtcga gcgccgagat tgagaaaaac gcaggtgaca
     3601 gcgacgtcat cgacgcccgt ggtcgggtcg tgttgcccgg gtttatcgac gcgcacgccc
     3661 acccgctttt tgccggaaat cgtctcgctg atttcgaacg ccgcgcgcgt ggtgaaactt
     3721 atgagcagat cgccgccggc ggcggcggaa tctggtcaac cgtggaaaag acgcgcgctg
     3781 cgactgaaac ggagcttctc aaccaagcca aaagacacgc gaactggttt ttgaaatgcg
     3841 gcacgacaac tgttgaagcg aaatcaggct acggactcac tcttgaggac gagctaaaga
     3901 tattgcgcgt gattcgccgg ctaaatgagg aaacccagat cgagtttgtt ccgacattcc
     3961 tgggcgcgca cgccattccg cgtgaatgcc gtgcagatca atacgttgag cttgtaatta
     4021 atgacatgtt gccgcgcgtt gtgtcagaga agctggcgga attctgcgat gttttttgcg
     4081 agcgcggtta tttcgacatt gagcaatcga gaaagatttt ggccgcggca aagaaactcg
     4141 gactcaagct gcgaatccat gccgatcaac tcactaattc cggtgcagca aaattggccg
     4201 ctgaattgga agcgaccacg gcggatcatt tggaaaaaac agacgggaag gggatcgcgg
     4261 cgatgaaatc tgctggtgtc cagccggttt tgctgcccgg gagcgtgtat gcgcttggat
     4321 cgacagatta tccatgcgcg cgagaaatga tcgaggccgg gctggcggtc gtcatcgcga
     4381 ccgattttaa ccctggctcg tcaccaagcc cgtcgatgcc gatgatgctg tcgttggctt
     4441 gcacccaaat gaaaatgtcg ccggcggaag caataacggc atcgacaatc aatgcagcct
     4501 acagcctgaa tcgcggcgac aagattgggt cactcgaaca gggaaagctg gccaactttt
     4561 cgatcttcga ttgcgaagat tatcgcgaag tcgcgtatta ctttggtttt ccgcaaacac
     4621 attcagttta cgtgcgcggt cggagagtct gccaagcctt gttcaaatca ggaggaggga
     4681 cgtgaaagaa gaactactga aactcgaaaa ggaattcacg caggcaattg tcaaaaacga
     4741 tgcggatgcg atcacgcgat tccttgccga cgactggatc atcatcgacg ccgatggagg
     4801 gatcgttgac aaggcgcgtt tcctgggagt gatcaagtcg ggcgctttga cacacgagat
     4861 gatggagtcc gatgaggttg cagttcgtat ctatggagaa gctgccgttg tcacggggct
     4921 gactgcgacc aagggaaaat tcatggggca agaatttagc actcgggagc gagcgaccga
     4981 cgtttttgta aagcaaagtg gacgatggcg atgcgtattc tcgcagctca ctcgattctc
     5041 taagaaatag ccgaggcgtt gccgcggcga acgcctgctc ccagaattgc ccacgaaaca
     5101 ctcgaaatac gctaaagatt tattctgttt gattttcttc ttttcgtgtc atttcgcgtg
     5161 tttagtgggc agtttttcgt tatttgcgtg gatgatcgtg cgcccgtagc gtcgctcatt
     5221 catgaaagtc gaagtcgaaa cgcagcccgg gtctgtctcg acgctccaga tcgagttacc
     5281 gccggaagag gtctcaaagg aatgggactc cattgcgaac agcttcgccc gccttgccaa
     5341 aattccaggt tatcgacccg gaaaagcgcc gcgaacggtc attgaaaaga gatttcgcaa
     5401 gcaaatcgag gaagagctta ccaaaaaact cgtctcgaaa agttatcacg aagcaatcga
     5461 acaacagcag ttgcgggtgg cctcgttggc caacatcgaa gacgtccagc tcggcgagga
     5521 caagtcgatg cgctttcgcg cgacggtggt gaccgcgcca gaattcgacc ttccggaata
     5581 caagaatatt cccatccagt tgcccgacac gaaagtgtcc gaggcggaca tcgataccgc
     5641 gctggagcgc gtgcgggatc aagccgctga ttttgtcgat gttccggagc gcgaactgca
     5701 aatgggagat tttgccgtga tcgactttga aggttcaatc gatggcaagc cgatcgccga
     5761 aatcgccccg caggcgagca ggaatctgca cggtggcaaa aaattctggc tgcatctcgc
     5821 tcccgaaaat ttccttccaa cgttttgcga gaaaatggtc gggcaaaaac gcggcgaaaa
     5881 gcggcagatc acaattgtat ttccggctga ttttccggtc aaagaactcg cgacgaaaga
     5941 ggcgaactac gcggttactt tgcaagagat aagggaaaaa gttctccccg cgctcgatga
     6001 cgctttggca aacaagctga ttcccggaaa gacgctggcc gatcttcgtc acctgatcga
     6061 acacgatctc gagcacgaaa aagagcacga ggtcgagcgc gcaaaggagg cgcagatcct
     6121 gaagtatttg catgagcaca tcagttttga attgccgcct gctttgatga aaaacgaaac
     6181 gcgacgggtt ttaggagagc ttgtgcaacg aaatcgggaa cgcggtgttt ctgatgagat
     6241 gctaaaggaa aaagagaaag agttgatcga aaccgccgcc ggtgttgccg tgcaccgact
     6301 aaagacgaat tttattctgc atcggatcgc tgagcgcgaa aacatcaaaa cgtcgcgcca
     6361 ggagatcgat gcgcgcatcc gcggagacgc cgctcgctac aacatttctc cggagaagat
     6421 gcgcaaggaa ctcgaggaac acgacgccct ggacagcgtt gccgaacaga ttctgctcgg
     6481 taagactctt gatttcctca aggcgaatgt tagcgtcgaa cggattcagg aacctcctgt
     6541 agtagaagaa aaatcatgag cgcatccaac gaaaatcgat cgcaatcggt gctggttccg
     6601 atggtggtgg agcagaccgg ccgaggcgag cgcagttacg atatttactc gcgattgctg
     6661 aaggatcgca tcgtcttcat cgggacaccg atggacgatc acatcgccaa tctggtcatt
     6721 gcgcagcttc tctttctgca aatggaggac tcgaaaaagg acatcaacat ctatatcaat
     6781 tcgccgggcg gttcggtgac ggccgggctc gcggtttacg acaccatgca gtttcttacc
     6841 tgcgatgtga cgacctattg tctcggtatg gctgcaagta tggccgcggt cttgctgtgc
     6901 gccggcacga agggtaaacg cttcgccctt ccgaactcag atatcatgat ccatcaggtt
     6961 tccggaggcg cgcagggcgc cgcgagcgac gtcgagcgcc aggtggatta catgttcaag
     7021 cttaagaagc gcctgatcaa gatcatcgcc cagcacacca gcaaatcgga ggaacaggtg
     7081 cggctcgatt cggaccgcga ttattacatg tccgcccagg aggcgaaaga atatgggctg
     7141 gtggatgaag tgattaaatc ccgcaaggaa gtgaagctcc ttgacggtgc gacgcctcct
     7201 tccggggacc gatcggtagc cgttgccgag gctgccttgc cgcgcaaagc cgggggataa
     7261 aagctccttt tctaaaaaca catatggcgc gcgcttccaa cctcaccatg tgctcgtttt
     7321 gcggcaagag ccatgccgag gtgcgcaaac taattgccgg gccgggcgtt tatatttgcg
     7381 acagttgcat caacgtttgc aaaagcatcc tcgataaaga gctcaatgaa gatgcgcgac
     7441 gtcagtcctc caatatccgt gtgccgaagc cggcggaaat ccggcgtttg ctcgaccaat
     7501 acgtgatcgg acaggacacc gccaaaaaga cgctttcggt ggcggtgcac aaccattaca
     7561 aacgcgtgct tcaaccttcc gcgccaggcg atgcgtcggc ggcctttcag ccggacccgt
     7621 ttgcggacgt tgaaattgag aagagcaaca tattgctaat cgggccgacc ggctccggaa
     7681 aaactttgct ggcgaaaaca ttggcccgga ttctcgatgt cccattctgc atcgccgatg
     7741 caaccacgct gacggaagcc ggctacgtcg gtgaagacgt cgagaacatt atcctgcgcc
     7801 tgctgcagaa tgctgattac gatgtcaaac gggcggagat cggcattgtt tacatcgacg
     7861 agatcgacaa gatcggccgc aaaaccgata acgtcagcat cacccgcgat gtttccggcg
     7921 agggcgttca gcaggcgctt ctgaagattt tggaaggcag cacctgcaat gttccgccgc
     7981 agggcggccg caagcatccg catcaggaat acatccaagt caacaccgag aagattctct
     8041 tcatttgcgg cggggcgttc atcgggctgg acaagattat tcaaaaacgc attggccaaa
     8101 aagtgatggg ctttggaagc ccgacactcg aagtcgagga gaccttgcga aagcaagcta
     8161 tccggcatgt tgaaccggaa gacctgctgg ctttcggaat gattcccgaa ttcatcggac
     8221 gcctgccggt ggtcagctct ctcaacgccc tgaccgagga agaaatggtg gcgattttga
     8281 ccgaaacgaa aaacgcgatg gtcaagcagt acaccaagct tttttcgatg gaaggcgtgc
     8341 ggctgacgtt gacgaaagac gctctgcgcg cactggcagc gcaggccgtc acgaagggca
     8401 caggcgcgcg cgcgctgcgt tcgatgctcg agcgaatcat gctcgacatc atgtacgacg
     8461 tgccgagtcg cgaagacatc gccgaagtga cgatcaaccg cgccgtggtc gaaggcagaa
     8521 agctcccgct cattcgcaag aagcaggaca aagacgcggc gtgattcgac ggaaacgccg
     8581 aacgttcaac attcaacgcc caacgttgaa ttcagatttc ggcttctgag ctcgacattg
     8641 ggcgttcggc gttgagcgtt ggacgttttc ttctgttcgc agcaaacttc aacacgcgca
     8701 atgagagtgg tcgttctcaa cactggcaca gagctcttgc tcggggacgt tcaggacgcg
     8761 catctggcgt ttatcgcgcg cgaaattctg ccactcggac tgcgtatcaa cgagcgacgg
     8821 acggttccag atggtccggc gattcgagat ggcctctcgg aattatttgg gcggtccgag
     8881 attttgtttg tgaccggcgg actgggtccg accagcgacg acatcacgcg agaaatcgtc
     8941 gcggatttac ttcgcctgga actacgtcag gatgcgcaac tgttggcttc gctgaaggag
     9001 agactcgcag cgcgcggaat caagtggact tccaggattg cccggcaggc cgaagtgcca
     9061 gctggcgcgc aagttcttcc aaacgaaaac ggaagcgcct ccggcttata tctgaagact
     9121 aacatcaatc ctttgattcc ttcgccccac gtttttgttc tgccggggcc gccccgcgag
     9181 ctccagccga tgtttcgcga gtccgtgacg tcgatcttac gctcaattgt gcaggttccc
     9241 gcatcgatcg agcgacggct ctataaaatt gcgtgcctgg gggaatcgac catcgaacag
     9301 gcaatcggcg acaaagttct cgctattccg gggatcgagc ttggttattg cgcgcgccct
     9361 ggcgaagtcg atatccggat tgtcggcgaa actgatgtcg tctcgcgcgc tgacgtaatt
     9421 atcaagaagg agctgggctc atcaatcttc tctgcgaacg acgaagccct ggaggaagtc
     9481 cttgtgaacc tcctcagcag ccgaaaggag acgctggcca tcgcggagtc gtgcacgggc
     9541 ggattgctgg cacatcgcat cacaaacatt ccgggcgcgt cgtcggtttt cctcgctgga
     9601 tacatttgtt atgcgaacga ggcgaagata gacatgttgg aagtcgatcc agggttaatc
     9661 gagaagcacg gcgctgtgag cgagccggtc gcgcgcgctc tggcccaaca cgcccgcatg
     9721 catgcacgat cgacctatgc tcttgctaca accggaattg ccgggccgtc gggtggttcg
     9781 cccgaaaaac cagtcggaac gatttacatt gcactggcca cgccggctga aatcgcggtg
     9841 aagaaattat tttttccgag cgatcgcgag acgtttaaac aaatggctgc ccaggcggct
     9901 tttgatctgc tgcgcagaaa ggtaattgaa acatagcctt tggctgtgtg cccagcggac
     9961 attttgtccg ctgttccggt tcagcggagt tcaactccgc tgggcgcaca gattcgaaat
    10021 ctatgttccg gtaacttcga cgggcgtgcc gactttcaca ttatcgtaaa atagcttcgc
    10081 tccgtccggc ggcatccgga tacagccatg cgatgcggga tatcctggca aaatcccgac
    10141 atgcattccg accccttccc ccgttagccg cataaaccac ctcat
//
LOCUS       DASQUQ010000184         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_697889, whole genome shotgun sequence.
ACCESSION   DASQUQ010000184 DASQUQ010000000
VERSION     DASQUQ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697889"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VE486_05235"
     CDS             complement(<1..485)
                     /locus_tag="VE486_05235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZA38523.1"
                     /translation="MIRRDYLIIGSGIAGASACEGIRRHDKRGTVTLIGAEAFPPYKR
                     WILSKSFLREKNPQLKKLQEPDERWYRAHKVETRFGTVVTQFNIDRRLAVLANGESIE
                     FTKACLAMGSRPVRPPVAGVGLGNVIYLRTLRDALALKEMADLEKSLMVVGGGLLACE
                     AA"
     gene            complement(577..1941)
                     /locus_tag="VE486_05240"
     CDS             complement(577..1941)
                     /locus_tag="VE486_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZA38524.1"
                     /translation="MLNYIWLALVLLAVAIGGWNDRFKEVTAGAFDGAATAVTIALGL
                     IGIMALWLGVMRLAERAGLVQKIARGLRPIMRRLFPDVPPEHPAMGSMVMNMSANMLG
                     LGNAATPLGLRAMRDLESLNPRPGVATNAMCTFLAINTSSVQLIPATAIAILAASGST
                     RPTAIVGTALLATLCAASVAIISVKLLEKLPTFRISPSNQRTKIEQDFEKAEIKEGAG
                     VNDPSYSSIVPQEPVKRLSIFSLLALMGLVVLFLVLFVRMVAPNLFGVPIAPETAAQN
                     IFVRSVNALSILAIPFLLSFFPLYAAARGVKVYDEFVEGAKEGFGVILKIIPFLVTML
                     VAIGMFKGAGGIDLLSRLLSPILTPLQFPTDLLPLALMRPLSGSATLALLTDIVHRLG
                     PDNLVSLTAATIYGSTETTFYVAAVYFGAVGIKQTRHAIPAGLLADLTGVVASVIICR
                     AVLG"
     gene            2102..2617
                     /locus_tag="VE486_05245"
     CDS             2102..2617
                     /locus_tag="VE486_05245"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZA38525.1"
                     /translation="MKYLLDFLKGKWLKHPLHPILVHVPMAMWPGALIFDLLSRWNGN
                     AMVRLSFYAIIFGLVASLLAVPTGVVDWTGIKKEKPAWKIGLYHMILNLVVAVLFAIN
                     LGLRVYTFGHDTIVTGAPLALSIIGTALLMGSAYLGGLMVYDYGISVARMSKKKWRKI
                     AETGGANLPPE"
     gene            2625..>2832
                     /locus_tag="VE486_05250"
     CDS             2625..>2832
                     /locus_tag="VE486_05250"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZA38526.1"
                     /translation="MLKDFLEGKPLRHPLHPILVHFPIGLFILSLLFDLGSLAFPSTP
                     NLVRDAFYAMVLGIIGALIAAVPGL"
BASE COUNT          622 a          835 c          775 g          600 t
ORIGIN      
        1 gccgcctcgc atgcgagtaa cccgccgccc acgaccatga ggctcttctc cagatcagcc
       61 atctccttta acgcgagcgc gtcacgaagg gttcgcagat agatcacatt accgagaccg
      121 actccagcga ccggcggtcg caccggacga ctccccatgg ccaggcacgc cttggtgaat
      181 tcaatgcttt caccattggc aagcacggcc aggcgtcgat cgatgttgaa ttgcgtcacc
      241 accgtcccga atcgcgtctc gaccttgtgc gcgcggtacc agcgttcatc gggttcctgc
      301 aattttttaa gctgaggatt cttttcacgc agaaagctct tcgagagaat ccagcgcttg
      361 taaggcggaa acgcttccgc gccaatcagc gtaacggttc cccgtttgtc gtggcggcga
      421 attccttcgc aggcgctcgc tccggcaatg ccgctaccga tgatgagata atcgcgtcga
      481 atcatttggg aaaagctcga cgataagaga tcaacttacc ggctggcgag ggaaaaattt
      541 gttcgtaggg cgcgaccgcc gggcgcgccg tcgaacctaa ccaagcaccg cccggcaaat
      601 tatcacagaa gcgacgaccc cggtaagatc tgcaagcaaa cccgctggaa tcgcatgccg
      661 cgtttgtttt attcctaccg ctccgaagta aactgctgct acgtaaaacg tcgtctccgt
      721 gcttccataa atcgtcgccg cagtcagact gaccaggttg tcgggtccaa ggcgatgcac
      781 aatgtccgtg aggagagcga gcgtcgcgct tccgctcaga ggccgcatca aggcaagcgg
      841 gagaagatcg gttggaaatt gcagcggcgt caaaattggt gaaagcaggc gactcaacaa
      901 atcgattccg ccagcgcctt taaacatccc gatcgctacc agcatcgtta cgaggaacgg
      961 aatgatcttc agaattacgc cgaaaccttc cttcgcgccc tcgacaaatt cgtcatagac
     1021 cttcacgccg cgcgccgctg catagagcgg gaagaagcta aggagaaatg gtatcgccag
     1081 aatcgacaac gcgttcacgc tgcggacaaa gatattctgc gccgccgttt cgggtgctat
     1141 tggaacacca aacaaattcg gcgccaccat ccggacaaaa agcaccagga agagaacaac
     1201 cagtcccatc agcgcaagta aactgaagat cgataaccgt ttcaccggtt cttgcgggac
     1261 aatgctgctg tagctggggt cgttgacccc ggccccttct ttaatctccg ctttctcgaa
     1321 atcttgctcg attttcgtcc gttgattcga cggcgaaatc cgaaaagtcg gcaatttctc
     1381 gaggagcttt accgaaataa tggcgacgct cgctgcgcaa agtgttgcaa gcagcgccgt
     1441 gccgacaatt gccgtcggtc gcgtcgatcc ggacgcggcc agaattgcga tcgcggtcgc
     1501 gggaatcaat tggacagagc tcgtgtttat tgccaggaac gtgcacatcg cattggtcgc
     1561 cacgccgggc cgcggattaa gcgactccag gtcacgcatt gcgcgcaaac ccagcggcgt
     1621 agctgcattg cccaagccaa gcatgttcgc cgacatgttc atgaccatgg aacccatggc
     1681 agggtgctca ggaggaacat ccggaaaaag ccggcgcatg attggacgca gaccacgcgc
     1741 gatcttctgc accagtccgg cccgttcggc caggcgcatc actcccagcc ataacgccat
     1801 gatgccaatt aaaccaaggg cgatggtaac ggccgtcgcc gccccgtcga acgcgcccgc
     1861 ggtcacctct ttaaaccggt cgttccaacc gccaattgcg actgcgagca acacgagcgc
     1921 gagccagatg taattcagca cgcgtaatgt tgtagccggg gtcgctgacc ccggccagcc
     1981 gatattgttg ttgcgttgtc gatctttctt tcagcaaact ggccatcgga tgaatgaatc
     2041 cgccactcaa cattagagac acgaccggga atccggctcc gcttggactg ctttcgtcgc
     2101 catgaaatac ctgctcgatt tcttgaaggg caagtggctg aagcatccgt tgcacccaat
     2161 ccttgtccac gtgccgatgg cgatgtggcc cggagcgctg atcttcgatc tcctctcccg
     2221 atggaacggc aacgccatgg ttcggctttc tttttacgcg atcattttcg gcttggtcgc
     2281 gtcgttgctg gccgtgccga cgggcgtggt ggattggacc ggcattaaga aagaaaaacc
     2341 ggcgtggaaa atcggcttgt atcacatgat cctgaatctc gtggtcgccg tgctgtttgc
     2401 gatcaatctc gggctgcggg tgtacacgtt tggtcacgac accatcgtca ccggcgcgcc
     2461 gctcgcgctc tcgatcatcg gcacggcgct gctgatgggc tcggcctatc ttggcgggct
     2521 gatggtttac gattacggca tcagcgtcgc gcgaatgtcg aaaaagaaat ggcgcaaaat
     2581 cgccgagacc ggcggcgcaa atcttccacc agagtaagat cgacatgctg aaagatttcc
     2641 tggaaggcaa accgctccgg catccgctgc atccgattct ggttcatttt cccatcgggc
     2701 tgtttatcct cagcttgttg ttcgatctgg ggagcctcgc ttttccatcg actccgaatc
     2761 tggttcgcga cgccttttac gcgatggtgc tcggaattat cggagcgcta atcgcggcgg
     2821 tgccgggctt gg
//
LOCUS       DASQUQ010000185         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1129087, whole genome shotgun sequence.
ACCESSION   DASQUQ010000185 DASQUQ010000000
VERSION     DASQUQ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129087"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..2165)
                     /locus_tag="VE486_05255"
     CDS             complement(159..2165)
                     /locus_tag="VE486_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38527.1"
                     /translation="MSGFFEEVKRRKVYRVAVAYIIAAGGVIQLASAAFPAWELPNWA
                     LRLVIVLLLIGFPIALILAWAFDVTPEGIRSAPSTAAPRTHRRRNVIMLVATGVMASA
                     IAGFFLLPRISSARKIDKSIAVLPFESLSDDKENAYFADGIQDDVLTNLSKISELKVI
                     SRTSVMPYRGKASNVREIGKALGVATILEGSVRRIGNRVRVNVQLINAENDEHIWAED
                     YDRELTDVFAIQTDLAHEIVNALQAKLSDAEKARMERKPTENGEAYLAFVQAHNLQAA
                     YEDFDKLKQSEQLYDRALELDPKFALALARYSQLESWIVRSFERTPARREKARSLAER
                     ALQLQPDLPEAHLALGFSYYYGDNNYDAALREFEIAQHGLPNESEAYLAIGAIQRRQG
                     KWKESTANLEKAATLSPNDSWVLQNLVFNYAMHRNFDAANKTIDRALQLKPEGLSLWE
                     IKAKLAVSEKGDLSVGENLLEKAKSIPMSKEEKLKLLGGQVDFLLLQRKYKEVLEMAE
                     KSSDDLFAAVPGSLAFKYFAIGLAQKGLHDETNARAALLKAKNICEEQLKQKPDEPDL
                     HVQLAKLNAWLGERDAALSEAQRATQLRPESKDAFEGPQITGQVAEVHAILGDNDRAI
                     ELLDGLLNRPSELTVASLKLNPAWDPLRNDPQFQALLNKYGGKS"
     gene            complement(2238..3170)
                     /locus_tag="VE486_05260"
     CDS             complement(2238..3170)
                     /locus_tag="VE486_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZA38528.1"
                     /translation="MASTINLLRLLADPTRLRLLLLLEQEELSVAELQQILGMGQSRI
                     SSHLAQLKRAGAVTDRRVGKNVYYGANHNGQRAQRERVAEITRLLARELPESSRDRTS
                     LKLVLHKRQDKARKYFDELAGKFGRSYVPGRSWKALAHTFISLLPPLTVADLGAGEGT
                     LSQLLAKNARKVIAIDNSPKMVEFGSQLAKKHGFRNLEYRLGDIEDPPIAKNSVDLAI
                     LSQALHHAIHPQRAVAAARRLLKRGGRLVILDLLSHRFERARDLYADHWLGFSEVQLH
                     QFLEKSDFRQIEVSVVSREKQSPHFQTVFATGVK"
     gene            3330..>3753
                     /locus_tag="VE486_05265"
     CDS             3330..>3753
                     /locus_tag="VE486_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HZA38529.1"
                     /translation="MSHRYIFSSESVTEGHPDKVCDTISDYMLDACLEQDPLSRVACE
                     TLAKGNLVVLAGEITSNAKFDFEERVREAVRSIGYVNGDCIFHADTCRVICEMSEQSR
                     DIKQGVDSAAADGKATAEQGAGDQGLMFGFACNETPELM"
BASE COUNT          811 a         1054 c         1001 g          887 t
ORIGIN      
        1 acagcgcgag atcgatcgtc gcactcgcgt tcgtttcaga atcttccaga acaacgtgcc
       61 gatgcacaac gccgccgcgt ccgggttccg aatgggccga ggcaatcgtc cagtccgcat
      121 gcgccgtgac cgcgacggcg agaaagagga gggcggccct agcttttgcc accgtattta
      181 tttagcaaag cctgaaattg cggatcgttt cgcaacgggt cccacgccgg attcaatttg
      241 agactcgcca cggtcagctc actcggccgg ttcaacaaac cgtccagtag ctcgatcgca
      301 cgatcattat cgcccagaat cgcatgaacc tcggcaactt gcccggtaat ttgcggcccc
      361 tcgaacgcat ctttactttc cggccgcagt tgggtggcgc gttgggcctc ggataaagct
      421 gcgtcccttt cgccaagcca cgcattcagt ttggccaact gaacgtgcag gtcgggttcg
      481 tccggttttt gtttcagttg ttcttcacaa atgtttttgg ctttcaacaa tgccgcgcgc
      541 gcattggttt cgtcgtgcag ccctttttgc gcaaggccga tagcaaaata tttgaaggcg
      601 agggatcctg ggaccgcagc aaaaagatcg tcagaagact tttccgccat ctccaacact
      661 tccttgtatt tgcgttggag cagcagaaaa tccacttggc ccccaaggag cttgagcttc
      721 tcttccttgc tcattggaat tgatttcgcc ttttccaaca ggttttcgcc gacgctcaga
      781 tctccttttt cgctgacggc aagtttggcc ttgatttccc aaaggctgag tccctcgggt
      841 ttcaactgaa gtgcgcgatc aatcgttttg tttgccgcat caaagttccg gtgcatggca
      901 taattaaaaa cgaggttttg cagcacccat gaatcatttg gactcaaggt tgcggccttc
      961 tcgagattgg cggtcgattc cttccatttt ccctgccggc gctggatcgc gccgatggcg
     1021 agataagctt cggattcgtt gggcaacccg tgttgtgcga tctcaaattc tctcagcgcc
     1081 gcgtcgtaat tgttgtcgcc gtagtaatac gagaatccca gggccagatg cgcttccggc
     1141 agatcgggct gcaattgcag cgcgcgctcg gcgagggagc gcgctttttc gcgtcgggcg
     1201 ggcgtgcgct cgaagctacg cacgatccag ctttccaact gcgagtagcg cgccagggcc
     1261 agcgcgaatt tcgggtcgag ctccagtgcg cgatcgtaga gctgttcgct ctgtttcagt
     1321 ttgtcgaaat cctcgtaagc ggcctgcaga ttgtgcgcct gcacaaaagc gagataggct
     1381 tcgccgttct cagtgggctt gcgttccatt cgcgcttttt ccgcgtcgga aagcttcgcc
     1441 tggagcgcgt ttacgatctc gtgagcgaga tcggtttgga tggcgaagac atcggtcaac
     1501 tcgcgatcgt aatcctcggc ccaaatgtgc tcgtcgttct cggcattaat caattgcacg
     1561 ttcacacgca cacgattgcc gatccggcga acgctgcctt ccaagatcgt agcgacgccg
     1621 agcgcctttc cgatctcgcg aacattggat gccttacccc gataaggcat gaccgatgtg
     1681 cgagagatta ccttgagctc gctaattttc gatagattcg tgagcacgtc gtcctgaatg
     1741 ccgtcagcga aataggcgtt ttctttgtcg tcgcttaaac tttcaaatgg caataccgcg
     1801 atcgatttgt cgatcttacg agcggaagaa atgcgcggaa gcaggaaaaa tcccgcaatc
     1861 gcagatgcca tcaccccggt tgcgacaagc atgattacgt ttcggcggcg gtgcgttctg
     1921 ggcgcagctg tactcggcgc agatcggatg ccttccgggg tcacatcgaa agcccacgcg
     1981 agaatgagcg cgataggaaa accgattagt aagagcacga tcaccagacg cagcgcccag
     2041 tttggcagtt cccaggctgg aaacgcggcc gaggccagtt gaataacccc gccagctgct
     2101 atgatatagg ctaccgccac ccggtacacc ttgcgccgtt tcacctcttc gaagaaaccg
     2161 ctcatagcaa cagatttgga ttcagcggga ttatagttca acggtgcaaa tatagacacg
     2221 aaaatgaagc tcgccgttca ctttacgccc gtggcaaaca ccgtctgaaa atgcggactc
     2281 tgtttttcgc gcgagacgac gcttacttcg atttgacgga aatcgctttt ttccaggaat
     2341 tggtgcaatt gcacttcgct aaatccaagc cagtggtcgg cgtagaggtc gcgggcacgt
     2401 tcgaaccggt ggctcaacag atcgagaatc accaacctgc cgccacgttt caggagccgg
     2461 cgtgccgccg cgacggctcg ctgcggatga atggcgtgat gtaaagcctg gctcaaaatc
     2521 gcaagatcga cactgttttt ggcgattggc ggatcctcaa tatcgcctag tcggtattcg
     2581 agatttctaa atccgtgctt cttcgctagc tgcgatccga actcgaccat tttcggcgaa
     2641 ttatcgatcg cgatgacttt gcgcgcattt ttcgccagca gttgcgaaag ggttccttcg
     2701 ccggcgccaa gatcggcaac cgtcaacggc ggcagaaggg agataaatgt gtgtgccagc
     2761 gctttccatg accggccggg cacataactt cggccaaatt tgcctgcgag ctcatcgaaa
     2821 tacttccgcg ccttatcctg acgtttgtgc aaaacgagct tcagactggt gcgatcacgt
     2881 gagctttccg gcagctcgcg cgccagcagc cgcgtgattt ccgccacccg ctctcgttgc
     2941 gccctttgtc cattatggtt tgctccgtaa taaacgtttt tcccgacccg tcgatcggtc
     3001 accgcgccgg cacgctttaa ctgtgcgaga tgacttgaaa tgcgggattg gcccatgccc
     3061 agaatttgct gcaactccgc caccgagagt tcttcctgct cgagaagaag caacaagcgg
     3121 agacgagtcg gatccgccag caatcgaagc aaatttatgg ttgacgccat gagatcccaa
     3181 cgatatatcg acgcatcatg atcggtcaat atgtcattga acggcgttac atcgttaaaa
     3241 atgttgcacc gttacaacgg ctgaagacgc ctcgcccaac ctgacctgat ttaacattgt
     3301 agccctttta acaaatcact cctcactcca tgtcgcatcg ttatattttt tcctccgaat
     3361 ccgttaccga aggtcatccc gacaaagtct gcgatacaat cagcgattac atgctcgacg
     3421 cctgtctcga gcaggacccg ctgagtcggg tcgcttgcga gacgctcgct aagggcaacc
     3481 tggtcgtgct cgcaggtgag atcaccagca acgcgaaatt tgatttcgaa gagcgagtcc
     3541 gcgaggcagt tcgcagtatt ggatatgtaa acggcgattg catttttcac gctgacacct
     3601 gccgggtgat ctgtgaaatg agcgagcaat cgcgcgacat taaacaggga gttgacagcg
     3661 ccgctgcgga cgggaaagcc acagccgagc agggcgccgg cgatcagggc ctgatgttcg
     3721 gctttgcctg caatgaaacg ccagagctca tgc
//
LOCUS       DASQUQ010000186         9830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_583303, whole genome shotgun sequence.
ACCESSION   DASQUQ010000186 DASQUQ010000000
VERSION     DASQUQ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583303"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VE486_05270"
     CDS             complement(<1..163)
                     /locus_tag="VE486_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HZA38530.1"
                     /translation="MSSEVRVRFAPSPTGYLHIGGARTALFNWLFARHHGGKFVLRIE
                     DTDQTRHVEE"
     gene            complement(225..1289)
                     /gene="tilS"
                     /locus_tag="VE486_05275"
     CDS             complement(225..1289)
                     /gene="tilS"
                     /locus_tag="VE486_05275"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZA38531.1"
                     /translation="MDWIQINPQLFSSFPPDSRYLVGVSGGRDSIALLDWLIHAGYRR
                     LIVCHLNHQLRGRSSNADARFVEKLAAKYDNKIVGQALRLPGSRTGKRSARPTTRIAH
                     IDPSTPLRTSFELGSANVRALAKRNKTSIETAARNARYSFFATVAKRRRCHTIFLAHH
                     ADDLVETFLINLFRGAGATGLAGMREVSTRRIEDVDLTIVRPLLSVWRDKIDSYVRER
                     RLKFREDASNKNLAPLRNRIRHRVIPHLEKTLGRKIRQNIWRTAMITADEENWIDNQL
                     PVSAHHDDQLKVRDFRGLPIALQRRAILKWLRAQNVSEIGFDVIERARSLLDPAIRVA
                     KVNLAQDRHLRRRAGKIFVD"
     gene            1363..1626
                     /locus_tag="VE486_05280"
     CDS             1363..1626
                     /locus_tag="VE486_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38532.1"
                     /translation="MSGVFRRKDQATMKSQTTYTLLVRSEEKNRDLLEAVVYALCILS
                     AIVAIWQFVDQSSRLSVDRLASRLHSASVVLQSTAESDLDARS"
     gene            1807..2436
                     /locus_tag="VE486_05285"
     CDS             1807..2436
                     /locus_tag="VE486_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZA38533.1"
                     /translation="MLQPQIESIPDRRTQILDAALVCFAKRGFHQASMHDISAEAGIS
                     VGLIYRYFENKEAVISAMADRHKKEIHEVLERAKQAPTLLESLEILFTAHCCEDAPQV
                     VSAFVADLYAEASRNQHVADLVRDVLQTAMDGVTDLIARSPEAQTAAHGLSPTELAEL
                     IYAVARGMLMLDVLRPQQMTAAERRERQLEVTRRLWGLLFSNAPEPVAI"
     gene            2595..3632
                     /locus_tag="VE486_05290"
     CDS             2595..3632
                     /locus_tag="VE486_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZA38534.1"
                     /translation="MTSTPNVMPPTTVSSATVKEEDWAPVLSAVGSISAVQGAVVSSE
                     LAGVVSQIAFENGRTAKKGDLLVQLDASAEEAQLKSAEADLQLARSDLERSRELVGRK
                     VISKAEIDAAESKFKQKEGSVEQMRSMIAKKTVRAPFDGQLGIRQVNVGQMINAGQQV
                     VPLTSLDPVFADFALPQQHLGQLKPGLEVHVTTDALPGRVFKGKVTAINSMVDSSTRN
                     ITLQATLENPDHALRPGMFAKAEVTLPEKHKALVIPGSAISYAPFGDSVFVIDKKKDE
                     KTGKESKVIRQQFVRVGEARGDLVAITQGLKAGETVVSTGVFKLRNGMTVTINNDLAP
                     KPQTNPKPVDS"
     gene            3648..6824
                     /locus_tag="VE486_05295"
     CDS             3648..6824
                     /locus_tag="VE486_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZA38535.1"
                     /translation="MKSFTDLFIKRPVLALVVSFVILIAGLQAMRTINVRQYPRSDIA
                     AITVSTVYVGADAELVRGFITTPLERAIAAADGIDYIQSQSKQGESLITARLKLNYDA
                     NKALSDISSKVDAIRRDLPPDAEIPVISIEAADSQFASAYLSFTSDILQPNEVTDYLV
                     RLVQPRLTAVGGVQKADILGGRTFAMRIWMKPDRMAALNISPAQVRTMLARNNFLSAV
                     GNTKGSLLQVNLTANTDLHTVDDFKKLVIRQEKGNLVRLSDIADVELGAEDYNAEVRF
                     SGQKAVFMGIWVLPNANSVDVIKRVRVEMDQIQKELPAGMKGEIAYDATAYINDAIHE
                     VVKTLIDTLLIVMVVIFLFLGSIRSVLVPIVAIPISLIGALFLMQLFGFSLNLLTLLA
                     IVLSVGLVVDDAIVVVENVERHLRDGMSRMNAALLGARELIGPIIAMTITLAAVYTPI
                     ALQGGLTGALFREFALTLAGAVFISGVVALTLSPMMSANLLRAGHVDKGFSGLVNRTF
                     DRFRDWYGSHLDRTLNARPAVYIFWAGISLLALLMFAAIPKMASKELAPTEDQGVIFG
                     IITAPANATIDDTIRYADAAGTVFQNIPDTRFTFQLTFPDNGFGGMVLKPWGVRETPT
                     KAYMPRVQAELGAIPGIQMFPIMPSALPGGDNFPVSFVITSTADQERILEFARQIFEK
                     AMQAHIFQFGDIDTKIDQPQSAIVFDHDKVAALGLDMQQVGADLSASIGGNYVNRFNI
                     EGRSYKVIPQIKRVDRLNPEQLTNIYVSGPNDQLIPLSTVASIKPKTVARSLNRMQQL
                     NAVTISGVPAVSLDAALKFLENEARNILPKGYVLDYTGESRQLRTEGSKFLGVFLLSL
                     VLIFLVLAAQFNSFRDPFIILLGSVPLAMFGAGLFMFLKMPFGEFFTDRWTTSFNIYS
                     QVGLVTLVGLVSKNGILIVQFANELQREGLSKLAAVAQAARIRLRPIMMTSVATVAGH
                     FPLTLVTGAGAAARNSIGLVLVGGMTIGTIFTLFIVPSLYMLIAKEHHEKSLMEPDLE
                     DVAEDVDLTPEFAPAGAPDGNGNGWKQG"
     gene            complement(6897..7076)
                     /locus_tag="VE486_05300"
     CDS             complement(6897..7076)
                     /locus_tag="VE486_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38536.1"
                     /translation="MAGCIRNGTNFLSEEDLDLRHLSEAELIAYWNLWLEQAQATNEL
                     DEKTYSHGVFETVPA"
     gene            complement(7182..7457)
                     /locus_tag="VE486_05305"
     CDS             complement(7182..7457)
                     /locus_tag="VE486_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38537.1"
                     /translation="MKLQINVPVSGLDLASLLQGEEGFFLCQNRGQNFITVAKAGHSI
                     AFGPTPIGRGIIEQPSATLGPEPWVVTKISTSTEFAEFRTAGPMANR"
     gene            7624..8760
                     /locus_tag="VE486_05310"
     CDS             7624..8760
                     /locus_tag="VE486_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZA38538.1"
                     /translation="MKRISRVGTKREPVIDPSLERPLLCLHAAMNLVSFWKAIQQLLS
                     AAIPNRLIGLMLQPNPILPMTARWTLPTRDGFFAAEPLKSYIAGQRRQRLVRISDLFS
                     NRSSLMKSAFYRRYMAPQKCAHGIGLLFWKDQKLICVIAIMRTATQGDFSLAEMKLLR
                     QLYPQFLTALCRLRSLEREHSVRMDLEKFLSRLPLPTILLRWDLKPIYQNQAARDFCA
                     VWEKGPGEAQLTKATSPIPFEILDRCRLLKQQWANARRPIAPQTGVKEQVHHPRSPHL
                     RATLHLKQLNSAGVARPHFLIECEDLHSSAGARAGSASARLPHLVRLTRREQEVARLV
                     CEGRSNQEIADDACLSLPMVKKHLHAVFRKLEVPSRSRLIALLR"
     gene            complement(8962..9330)
                     /locus_tag="VE486_05315"
     CDS             complement(8962..9330)
                     /locus_tag="VE486_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38539.1"
                     /translation="MVKKIINALAFAVIAPLAFAQTSPTTTKQKTRTQPTTRTEIIRA
                     STAAPGTVTTFEPGEVIVVGSENGHDTFSYVLDETVRYVNKAGRKIDEHRIKPGTRVH
                     VYYDDTGETPVVDRVVVDER"
     gene            complement(9453..9659)
                     /locus_tag="VE486_05320"
     CDS             complement(9453..9659)
                     /locus_tag="VE486_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38540.1"
                     /translation="MSYACADSPPEGFAAEALQERREQVGQTLPGSAFSYISIDLANK
                     SAFAWSSACHVNDLQARAIAQILN"
     gene            complement(9749..9820)
                     /locus_tag="VE486_05325"
     tRNA            complement(9749..9820)
                     /locus_tag="VE486_05325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9785..9787),aa:Arg,seq:cct)
BASE COUNT         2152 a         2650 c         2771 g         2257 t
ORIGIN      
        1 cttcttcgac atggcgcgtc tggtcggtgt cttcgattcg caaaacgaac tttccgccgt
       61 gatgccgcgc aaaaagccag ttgaaaagcg ccgttcgcgc gccgccaatg tggagatagc
      121 cagtaggcga gggggcgaaa cggacgcgga cttcagagct cattggagtg atggagtatt
      181 ggaatagtgg agtaatgcat tttttccatc cctccaatac tccattaatc cacgaaaatc
      241 ttcccggcac ggcgacggag atgtcgatcc tgcgcgagat tgaccttcgc aacccggatg
      301 gccggatcaa gcagcgagcg ggcacgttcg attacgtcga atccaatctc ggagacattt
      361 tgcgcgcgca accatttcaa gatcgctctg cgctggagcg caatgggcag gccccggaaa
      421 tctcgcacct tcaactggtc gtcatgatgg gcagaaaccg gcaattgatt gtcgatccag
      481 ttttcttcgt cggcagtaat catggctgtt cgccaaatat tctgacgaat cttccgcccg
      541 agagtctttt ccagatgtgg aatcacgcgg tgccgaattc gattgcgcag cggcgcgaga
      601 tttttgttgg atgcgtcttc gcggaatttg agccggcgct cgcgcacgta actgtcgatc
      661 ttatcacgcc agactgagag cagaggccgc acaatcgtca gatcgacatc ttcgatgcgg
      721 cgggtggaga cttcccgcat cccagccagg ccggtcgctc cggcgccgcg gaatagattg
      781 atcagaaagg tctcaacgag atcgtcggcg tgatgcgcga gaaagatagt atggcatcgt
      841 cgccgtttgg ctactgtggc gaagaaagag tagcgcgcat tgcgggcggc ggtctcaatg
      901 gaagttttgt ttcgttttgc gagcgcacgc acgttggccg agccgagctc aaagcttgtc
      961 ctgagcggag tcgaaggatc gatatgcgca atccgcgttg tagggcgagc gctccgcttg
     1021 cctgtcctcg aaccgggcag gcggagcgcc tgccctacaa ttttattgtc gtacttggcg
     1081 gcgagttttt cgacaaagcg cgcatcggca ttactcgacc ggccacgcaa ttgatgattc
     1141 aagtggcaga cgatcagtct gcgataacca gcatggatca gccagtcgag cagcgcgatg
     1201 gaatcgcgtc cgccggaaac gccgaccagg tagcgcgagt cgggcgggaa actgctaaac
     1261 aattgcggat taatttgaat ccaatccatt tcgtcgtcgc atccaatagt cgatgcccaa
     1321 acatgagtct tcaatgctta gtcgagttag acaaaagcgg aagtgagcgg cgtctttaga
     1381 agaaaggatc aagcaactat gaagagtcaa actacttaca cgctcctggt tcgctcagaa
     1441 gaaaagaacc gggacctgct ggaagctgtg gtttatgcac tgtgtatttt gagcgcgatc
     1501 gtcgcgatct ggcaatttgt tgaccaatca agtcgacttt cggtcgatcg cctggcatcg
     1561 cggctgcatt ccgcatccgt cgttttgcag agcactgctg aatctgactt ggatgcgcga
     1621 tcttgagagc gcgcgcctga tttcgctggc agcctgcgct cccagcgacg gcaaaagcac
     1681 tgcctggagt ggtcctcacc cgaaagaggg aacgagaagt cgctcgaagg tggcattaca
     1741 aaatcccgcc aatgcgcaag gtggcgggtt gacaatgaat gaacgttcat tcatcttcct
     1801 aaccttatgt tacagcccca gatagaatca atccccgatc gccgcaccca aatccttgat
     1861 gctgccttgg tctgcttcgc caaacgaggc ttccaccagg ccagcatgca cgacatcagc
     1921 gccgaggccg gtatcagtgt cgggcttatc tatcgctatt tcgaaaacaa ggaagcagtc
     1981 atttccgcaa tggccgaccg gcacaagaaa gaaatccacg aagtactgga gcgcgcgaaa
     2041 caggcgccaa cgctgcttga gtcgcttgag attttgttca ccgcgcattg ttgcgaagac
     2101 gcgccgcagg tggtttctgc gttcgtggcc gatctttatg ccgaagcctc gcgcaatcag
     2161 cacgtggccg atttggtccg cgacgttttg caaacggcga tggatggggt gacggacttg
     2221 attgcacgtt cacctgaagc gcaaaccgcg gcgcacggcc tgagcccgac cgagctggca
     2281 gaactaattt acgccgtcgc ccggggaatg ttgatgctgg atgtcctgcg accgcagcag
     2341 atgacagccg cggaacggcg ggagcgacag ctagaagtga cgcgcaggtt gtggggactc
     2401 ttgtttagca atgcgcctga accggttgcc atatgatcac gatcgtaatt ctaattataa
     2461 ccgttctaac gctcttgttt gcgattggcg cggtggtgtc ttttgcattg agggcgccta
     2521 ctgcaggagc gatttttggg gttcttgccc tgtttctggc gttagcagac ctcaaagcat
     2581 ttcaaattcg aaagatgacg tccacaccga acgtgatgcc gccgacaacg gtatcgagcg
     2641 caactgtgaa agaagaagat tgggcgccgg tactttcggc agtcggttcg atctcggccg
     2701 tgcaaggcgc ggtcgtttcc agtgagcttg ccggagtcgt cagtcaaatc gcatttgaaa
     2761 acggtcgcac ggcgaagaaa ggcgatttgt tggtccaact cgacgcgtcg gccgaagaag
     2821 cacaattaaa gtctgccgaa gccgatcttc agctggcgcg ctccgatctt gagcgatcgc
     2881 gcgagttagt tggtcgcaaa gttatttcga aagcggagat cgatgcggcg gaatcgaagt
     2941 tcaaacaaaa agaaggaagc gtcgagcaaa tgcgctcaat gattgcaaaa aaaaccgtgc
     3001 gcgcgccgtt cgatggccaa ctcggaattc gccaggtgaa tgtcggacaa atgatcaacg
     3061 cgggccaaca agtggtgcca ctcacttcgc ttgatccagt cttcgccgat ttcgctttgc
     3121 cgcagcaaca tcttggtcag ttaaagccag gcttggaggt gcacgtaaca acggacgcgc
     3181 ttcccggccg tgtctttaag ggaaaggtga ccgcgattaa ttcgatggtc gattcgagca
     3241 cgcgcaacat cacgttgcag gcgacgttgg aaaaccccga tcacgcattg cggcccggaa
     3301 tgttcgcgaa agcggaagtc acattgcccg aaaaacacaa ggcgctcgtc attccgggat
     3361 ccgcaatttc gtacgcgccg ttcggtgact cggtttttgt aatcgacaaa aagaaggacg
     3421 agaagaccgg caaagaatcg aaagtgatcc gacagcaatt tgtgcgcgtt ggtgaagctc
     3481 gcggcgatct cgtcgcgatt acgcaaggat tgaaagccgg cgaaaccgtt gtgagcacag
     3541 gagttttcaa gctccgaaac ggaatgaccg tgacgatcaa taacgatttg gcgccgaagc
     3601 cgcagacaaa tcccaagcca gtggattcat aaccatcgga gatcgacatg aaatcgttca
     3661 ctgacctttt catcaagcgc ccggtgttgg cgttggtggt cagctttgtg atcctcatcg
     3721 ccggtttgca ggcgatgagg acgatcaacg tacgccagta tccgcgaagc gatatcgcgg
     3781 caatcacggt aagcacggtt tacgtcggcg cggacgcgga gcttgtcagg ggatttatca
     3841 cgacgccgct cgagcgcgcg atcgcagcgg ccgatggaat cgattacatc caatcgcaga
     3901 gcaaacaggg cgaatcactg atcacggctc ggttgaaatt gaattacgac gcgaacaagg
     3961 cgctctcgga tatcagctcg aaagtggacg cgatccggcg cgatttgccg ccggacgcgg
     4021 agattccggt catcagcatt gaggcggccg attcccaatt tgcttcggcc tatctcagtt
     4081 tcacctccga cattcttcag cccaacgaag taaccgatta tctcgtgcga ctcgtgcagc
     4141 cgcgtctcac cgcggtcggt ggcgtgcaga aagcggacat tctcggggga cgcacatttg
     4201 ctatgcgcat ctggatgaaa ccggaccgga tggcagcgtt gaatattagc ccggctcaag
     4261 tgcgcacgat gttggcgcga aataattttc tctccgccgt cggtaacacg aaaggctcgc
     4321 tgctgcaggt caatctcacc gcgaacaccg atctgcacac agtggacgat ttcaaaaagc
     4381 tggtcattcg ccaggagaaa ggaaacctcg tcaggctgag cgacattgcc gacgtcgagc
     4441 tgggcgctga agattacaac gccgaagtac ggttctccgg tcaaaaggcg gtcttcatgg
     4501 gcatttgggt tttgccaaac gcgaactcgg tggacgtgat caagcgtgtc cgcgtggaga
     4561 tggaccaaat ccaaaaggaa ctcccagccg gcatgaaagg tgaaatcgct tacgacgcca
     4621 cggcttacat caatgacgcg atccacgaag tcgtaaaaac tttgatcgac acattgctca
     4681 ttgtgatggt cgtgatcttt ttgttcctcg gctccatccg ctcggtgcta gtgccgatcg
     4741 tcgcgattcc aatttcgctg atcggcgcgt tgtttctaat gcagctgttc gggttcagcc
     4801 tcaacttgct gacgctgctg gcgattgtgc tttccgtcgg attggtggtc gatgacgcga
     4861 tcgttgtggt cgaaaatgtg gagcgacatt tgcgcgacgg tatgtcgcgc atgaacgcgg
     4921 cgctcctcgg tgcgcgagaa ttgatcgggc cgatcatcgc gatgacgatc acgctcgccg
     4981 ccgtttacac gccaatcgcg ctgcaaggtg gattgaccgg cgcacttttc cgcgagttcg
     5041 cgctgactct tgccggagcg gttttcattt caggcgtcgt cgcactgacg ctttcgccga
     5101 tgatgtcggc aaatcttttg cgcgccggcc acgtggacaa aggattcagc ggcctggtta
     5161 atcgcacgtt cgaccgtttc cgggattggt acggaagtca tctggatcgc acgctgaatg
     5221 cgcggcccgc ggtttatatt ttctgggccg gcatttcgct cctcgcgctc ttaatgttcg
     5281 cggcgattcc caaaatggcc tcaaaggaat tagcgccgac ggaagatcag ggcgtgattt
     5341 ttggaattat tacagcgccg gcgaacgcga ccatcgacga cacgatccgt tacgcggatg
     5401 ctgccggaac agtcttccaa aacattcccg acacgcggtt cacatttcag cttacatttc
     5461 cagataacgg atttggcgga atggttttga aaccgtgggg cgttcgcgaa acgccgacca
     5521 aggcctacat gccccgagtt caagccgaac tgggtgcgat tccggggatc cagatgtttc
     5581 cgatcatgcc ttcagcgctt cccggagggg ataattttcc ggtcagcttt gtgatcacat
     5641 caaccgccga ccaggaacgc attctcgaat tcgcgaggca aattttcgag aaagcgatgc
     5701 aggcgcatat tttccaattc ggcgatatcg atacgaagat cgaccagccg caatcggcga
     5761 tcgttttcga tcacgacaaa gtcgcggccc tgggcctcga catgcagcaa gtgggtgccg
     5821 atctctcggc cagtattggg gggaattacg ttaatcgatt caacatcgaa ggacgcagtt
     5881 acaaagtgat tccgcagatc aagcgcgtcg atcggttgaa tccggagcag ttgacaaata
     5941 tttacgtgag cgggccgaac gatcagctta tcccgctgag cacagtcgct tcgattaaac
     6001 ccaaaaccgt tgcgcgctcg ctcaatcgga tgcaacaact caacgcggtc accatcagcg
     6061 gggtgcccgc cgtttcgctt gatgccgctc tgaaatttct ggagaacgag gcgcgtaaca
     6121 ttcttccaaa aggttacgtc ctcgattata cgggagagtc gcgccagttg cgcaccgaag
     6181 gcagcaagtt tctcggcgtg tttttactat cgctggtgct gatctttctc gtcctggcgg
     6241 cgcagttcaa cagcttccgc gatccgttca ttattctgtt aggatcggtt ccgctcgcca
     6301 tgttcggcgc gggtctattc atgtttttga agatgccgtt cggcgaattc ttcacggata
     6361 ggtggacgac ttcgttcaac atctactcac aagtcggtct cgtcacgctc gtcggcctcg
     6421 tttcgaaaaa cgggatcctg attgttcaat ttgcgaacga attgcaacga gaaggcttgt
     6481 ccaagcttgc ggccgtcgcg caagcggcgc gcattcgttt gcgtccaatc atgatgacga
     6541 gcgttgcaac tgtcgccggt cactttccgc tcacgctcgt gaccggcgct ggcgctgccg
     6601 cgcgaaattc gatcggcctc gtgctcgtcg gcggaatgac gatcggaacg attttcactc
     6661 tgttcattgt gccgtcgctt tacatgctca tcgcaaagga acatcacgaa aagtctctga
     6721 tggaacccga cttggaggat gtggccgaag atgtcgatct tacgccggaa tttgcgccag
     6781 caggcgcgcc ggatgggaac ggcaacggct ggaagcaggg ttaaaatgtt aaaaagggtt
     6841 acaacgttaa atcggtcggc gcttttctga tttaaccatt caaccaacgc gcggcattac
     6901 gctgggacgg tctcgaacac gccgtggctg taggtttttt cgtcgagctc gttggttgcc
     6961 tgcgcctgtt cgagccaaag attccagtac gcgattagct ctgcttccga tagatggcgc
     7021 aaatcgagat cttcttcgct caggaaattc gtcccgttcc gaatgcatcc agccaattta
     7081 tgtctcaagg gggaagaaac aaagcaaaaa gccccggcga gttttctcgc cagggctttt
     7141 gattttttct ttgcggcttt gatgccttgc cttagcgcat tttatctgtt ggccattggc
     7201 ccagctgtcc ggaattcggc aaactcggtc gatgtgctta tcttcgtgac aacccagggc
     7261 tctggtccaa gcgtcgctga tggctgttcg atgatgcccc ggccgattgg cgtcggccca
     7321 aaggcgatcg agtgaccggc ctttgccaca gtaatgaagt tttgccccct gttctggcag
     7381 aggaaaaacc cttcttcacc ctggagcaag cttgccaagt ccaggccgct aaccggtacg
     7441 ttgatttgga gtttcatttg ctgtgtttcc tttcttgttt aaggttcaat ttcgcgcacc
     7501 gctggatgat cgccaggtaa cttaggttgc ctttttaaat cgcccgattg tcgaacgttt
     7561 caggaatgag cgcagccttg ggttggcgca aacgaatagc ggttgcgcga ggaggagccg
     7621 cgaatgaagc gaatctccag ggttggcaca aagcgcgagc cggtgatcga tccgtcactg
     7681 gaaaggcctc tgctctgcct gcacgcagcc atgaatctcg tttcgttctg gaaagccatc
     7741 cagcagttgc tttctgctgc gatcccaaat cgcttgatcg gtctcatgct tcagcccaat
     7801 ccaattttgc cgatgaccgc gagatggaca ctgcccacgc gtgatggttt tttcgccgcg
     7861 gagccgctca aaagttatat cgccggtcaa cggcggcagc ggttggtgcg gattagcgac
     7921 ctcttttcaa atcgaagcag cctgatgaaa tcggctttct atcgccgtta catggcgccc
     7981 caaaaatgcg cacacggtat cggcctcctt ttttggaaag accagaaact tatttgtgtg
     8041 attgcaatca tgcgcacggc gacgcagggc gacttttcac tcgccgagat gaagttgctc
     8101 cggcagcttt acccgcaatt tctgaccgcc ctttgccggc tcagatcgct cgagcgcgaa
     8161 cactcggtgc ggatggatct tgaaaaattc ctgagccggc tgccattacc gacgatcctc
     8221 ttgcgatggg acttgaagcc gatttatcaa aaccaggccg cgcgcgactt ctgcgcggtg
     8281 tgggaaaaag gacccgggga ggcgcaactg acgaaggcga cctcaccgat tccattcgag
     8341 attttggatc gatgtcgtct gctcaaacaa caatgggcga acgcgcgacg ccccattgcg
     8401 ccgcaaacag gcgtcaaaga acaggtgcat catccgcgat cgccgcattt gcgagcaacg
     8461 ctccatctga aacagctcaa ctcagccggc gtggcgcggc cgcattttct tatcgagtgc
     8521 gaagatttgc actcttccgc tggtgcgcgc gctgggtcgg cgagcgcccg tttgccgcat
     8581 cttgtccgac tcaccaggcg cgaacaggaa gttgcgcgcc tggtctgcga agggcggagt
     8641 aaccaggaga tcgctgatga cgcgtgcttg agtttgccga tggtgaaaaa gcatctccat
     8701 gcggtcttcc gcaagctcga agtgccaagc cgcagccggc ttattgcgct gctgcgatga
     8761 atcgcgcggc gggcgaggcg atttgctcgg ttcgcgtgtc gcgctaagat cgatatctaa
     8821 tctgacttcc gtaatctccc cgtagttgcc ccaaggatga aagaacgggt ggcacaaatt
     8881 ttcggcctca cgaggagacg atcagcagtg cctccggaac actcaggacc ctgcggcctt
     8941 gagtttgcgg attattttcg cttaacgttc gtcgaccacc acacgatcga ccacgggggt
     9001 ttcgccggtg tcgtcataat agacatgaac gcgagtgccg ggtttgattc ggtgctcgtc
     9061 gatctttctg ccggctttgt tcacgtaacg aaccgtctcg tcgagcacgt aactaaaggt
     9121 atcatgcccg ttttcagagc cgactacgat tacttcgccc ggctcaaacg tggtgacagt
     9181 gccgggggca gctgtcgatg ctctgattat ttccgtcctc gttgttggtt gagtacgtgt
     9241 cttttgcttt gtagtcgtcg ggctcgtttg cgcaaacgcc aaaggcgcga tcactgcgaa
     9301 agcgagagcg ttgattattt tcttcaccat gattgcctcc cctttttacg ttgatgtttt
     9361 accccaaagc ttcgtggttt aggattcgac cccgaatgct ttcggggttg ccgattccac
     9421 actttgaccg accggctacg gcgccgttcg aactaattca gtatttgtgc gattgcgcga
     9481 gcctgcagat cgttcacgtg acacgccgat gaccaggcaa acgccgactt gttcgccaga
     9541 tcaattgaaa tgtatgaaaa tgcggacccc ggcagggtct ggccaacctg ctcccgtcgc
     9601 tcttgcagag cctcggcagc aaatccttcc ggcggcgagt ctgcgcaggc gtagctcatg
     9661 acgctgatgc gatgaatccc ccggcgcgcg cgaccgtgca gacgggtagc ttcgtccgcg
     9721 gacgttgcaa gcggcgctaa caagaaaata tccccggcgg gaatcgaacc cacatctaag
     9781 gtttaggaaa cctccgttct atccgttgaa ctacggggac gcgtttcaga
//
LOCUS       DASQUQ010000187         3722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_515521, whole genome shotgun sequence.
ACCESSION   DASQUQ010000187 DASQUQ010000000
VERSION     DASQUQ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515521"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VE486_05330"
     CDS             <1..226
                     /locus_tag="VE486_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38541.1"
                     /translation="QFEHAYRTGYSAFLKYPGKKFDEVEESVAADYDTEKPGSALPYD
                     TVRPAISSVWDRMAGVIGPRDPDRGIRGAI"
     gene            675..1577
                     /locus_tag="VE486_05335"
     CDS             675..1577
                     /locus_tag="VE486_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZA38542.1"
                     /translation="MTNRVLVFAVAVTLLTVASVCADDNVREVQTKLHDAGFYRGEID
                     GAYSSQLTAALSRYQIRNGLPITGQLDAETSKALGAKPAVTTTAAEPAQSSEARRQLR
                     KRDQQFLAKTNARAAASPSANETPGTSTGTTNDSAEKAATTRPGTQSADSSLPQEPPS
                     GTAPISADARTSSPAADFSAERVRDYVAAFVLAGLDRQAGAEIDFFADRAAYYDSGMI
                     SREKIRQDLQRYAERWPERQFWIAGNISVEPQSENRVRVTFPLEFKLRNGTETTSGKV
                     DKSLILEAAGDDLQIVAVSERKAE"
     gene            1639..3063
                     /locus_tag="VE486_05340"
     CDS             1639..3063
                     /locus_tag="VE486_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZA38543.1"
                     /translation="MLDPNADQIRDWGDSVLELMADYLGDLRDSHVYGHMSSREIRDR
                     LDPTLPTKGIDFDGLLKVFRETVIPFSRQNAHPRMFGYVQSPGTSLAAFADLLASTLN
                     ANLTVWRSAPAPVELERLTIDWIRQILGFNAEAGGLFVSGGSMANLAAIAAARQAKDH
                     SSSGRLRIYASSETHFSIAKAAALLGIGRENVRHVAVDERFKIRVDDLVAKITADLEA
                     GYVPFCVVANAGTVNTGAVDPLTEIRKIADRFQLWMHVDGSYGALAVLAEWARKLFAG
                     IEQANSVALDPHKWLYLPVDVGCVIYREPEIARAAFAHEAEYTRIIGQEADEAFAFWD
                     YGPELSRRFRALKVWMLLKGVGLDSLGEAIESNLACARYFESMVRASDDFEMVAPVEL
                     SIFCFRHVPVQRRSESPKAIDAFNERLLVALQRDGSSYLSNATLGDRFALRGCVLNYR
                     TTLRDMEILLDDLRRVARSLPTSA"
     gene            complement(3100..3408)
                     /locus_tag="VE486_05345"
     CDS             complement(3100..3408)
                     /locus_tag="VE486_05345"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZA38544.1"
                     /translation="MPRFRETIPVDDYVLDVLMRDLVGHDHQPASYLVYLYLYGQAAR
                     AKGKPVPASLRRLADATGLSKSAVQSALENLRRRELIVTTSDHATAVPRHRILRHWRK
                     "
BASE COUNT          772 a         1068 c         1086 g          796 t
ORIGIN      
        1 acaattcgag cacgcgtatc ggaccggtta cagtgcgttc ttgaagtatc ccgggaagaa
       61 attcgatgaa gttgaagagt ccgttgcagc cgattacgac acggagaagc cagggtcggc
      121 attgccatat gacacggtgc gccccgcaat aagctcggtc tgggacagaa tggccggtgt
      181 tatcggcccg cgcgatccgg accgcggcat acgcggtgcg atttgaaact cgctcgtttc
      241 cctctctcgc tcgtcgctac ctcgcgactt taccttctat ctcggaaatc cctttcctcg
      301 attgaaactc gttcgcctcc caacttgctc gtcgctaccc tcgcgacttt gccttgacca
      361 ctcgcactcc ccgtgcttgt gccagctacc tagctgcgct gccgtctacc cgcgcgattc
      421 ccgatcgcga cgtgttctat ctcgctgatc caatcagctc cattgaaact cgctcgctgc
      481 ccgcgctcgc tcactcctac cctcgtcgct ttaccttcca tctcgctgat cccgtcagct
      541 cgattgtagc ttatcaatcc ggcagcaact gccagggaaa gctgcgttcc ggaggcggtg
      601 atcaagcaat gttgattgct cgaggccaca cttttaagca ggagcagccg acaggcggcg
      661 ttcgattaaa atgtatgacc aatcgtgtgt tagtttttgc cgtcgcggtc acgcttttga
      721 cggtcgcatc ggtttgtgcc gatgacaatg tgcgggaggt gcagacaaaa ctgcatgacg
      781 ccgggtttta tcgtggcgag atcgacggcg cctatagcag tcagctgacc gcagccctga
      841 gccgttatca gattcgcaac gggttgccca tcaccgggca gctcgatgcc gaaacgagca
      901 aggcgctcgg cgcgaagccg gcggtaacga cgaccgcggc tgagcccgcg caaagttccg
      961 aagcccggcg gcaattgcgc aaacgcgatc agcagtttct cgccaagacg aacgcgcggg
     1021 ctgcagcgtc gccaagcgcc aacgaaacgc caggaaccag caccggaacg acaaacgaca
     1081 gtgcggagaa agctgcgaca acacggccag gcacgcagtc ggccgattcc tcgctgccgc
     1141 aagaaccgcc ttcgggtacc gcgccgatct ccgccgacgc ccgcaccagt tctccggcgg
     1201 cagacttcag cgcggaacgc gtgcgcgatt atgtggctgc gtttgtcctg gccggactcg
     1261 atcggcaggc cggcgccgaa atcgattttt ttgccgatcg cgccgcgtac tacgacagcg
     1321 gcatgataag ccgggaaaag attcggcagg atttgcagcg ctacgctgag cgctggccgg
     1381 agcggcagtt ctggatcgcc ggaaatatca gcgtcgaacc gcagagtgaa aaccgggttc
     1441 gggtgacgtt tcctctcgaa ttcaagttgc gcaacggcac cgagacaaca tccggcaagg
     1501 tcgataagag ccttatcttg gaagccgctg gcgacgattt gcagatcgtc gcggtgagtg
     1561 agcggaaggc tgagtgagtg tccactgcgt cggtgcgaag gctgacaacg ccatcggcgc
     1621 gacgcatact acgtggaaat gcttgatcct aacgccgatc aaatacgcga ttggggcgat
     1681 tccgttctcg aacttatggc cgattatctc ggcgatctcc gcgacagcca cgtgtatggc
     1741 cacatgtctt cacgcgagat tcgcgaccgg ctagacccga cgcttccaac caaaggaatc
     1801 gatttcgacg gactgctgaa ggtttttcgc gagaccgtca tcccgtttag ccggcagaac
     1861 gcgcatccgc gcatgtttgg ctatgtgcaa tcgcctggga cgtcccttgc ggcgttcgcc
     1921 gacctgctcg cctcgacgct aaatgcgaat ctcacagttt ggcgttccgc gccggcgccg
     1981 gtggaactcg agcgcttaac gatcgattgg atccggcaaa ttcttggatt caatgctgaa
     2041 gcgggcggac tctttgtcag cggtggatcg atggccaatc tcgccgcaat tgccgcggcg
     2101 cggcaggcga aagatcactc gtcgtcaggt cgcttgcgca tctatgcgtc gagcgagacg
     2161 catttttcca tcgccaaggc agctgcgctt ttgggaatcg ggcgggagaa tgtgcgccac
     2221 gtagccgtcg acgagcgctt caaaatccgg gtcgacgatc ttgttgcaaa aatcactgct
     2281 gacctggagg cgggctacgt tccattctgc gtcgttgcga atgccggaac cgtgaacacg
     2341 ggcgcggtcg atccgttaac ggagattcgg aaaattgcag accgcttcca actttggatg
     2401 catgtggatg gaagttatgg cgccctggcc gtcctcgcgg aatgggcgag aaaattgttc
     2461 gccggcattg agcaggcgaa ttcggtcgcg ctcgatcctc acaaatggct ttatctcccg
     2521 gtggacgttg gctgcgtgat ttaccgcgaa ccagaaatcg cgcgggccgc ttttgcccac
     2581 gaggccgaat atactcgcat tattgggcag gaggcagacg aggccttcgc attttgggac
     2641 tacgggccgg aactgtcgcg gcgtttccga gctcttaaag tatggatgtt gttaaaagga
     2701 gttggcctcg attccctggg cgaagcgatt gaaagcaatc ttgcctgtgc gcgctacttc
     2761 gagtcgatgg tccgagccag cgatgacttc gagatggtcg cgccggtgga gctgtcgatc
     2821 ttctgctttc gtcatgtgcc ggtgcagcgc agaagcgaat cgcccaaggc aatcgatgcc
     2881 tttaatgaac gcctcttagt tgctctgcaa cgagatggca gttcgtatct atccaatgct
     2941 acgctcggcg atcgcttcgc tctgcgcggt tgcgtgctga attatcggac cactttgcgc
     3001 gatatggaga ttctcctcga cgatttacga cgcgtagcaa gatcgttacc gacttctgcc
     3061 tagaatccat ctcataaata gggaaaatct ttcgattaat tatttgcgcc aatggcgcag
     3121 gatgcgatgc cgcggcacgg cggtggcgtg atcgctcgta gtgacgatta attcgcgccg
     3181 gcgcaggttc tcgagcgcgc tctgcacggc gctcttggaa aggccggtcg cgtccgccag
     3241 cctccgcaaa ctcgcgggca ccggtttccc tttggcgcgc gctgcctgcc cgtagagata
     3301 cagataaacc agatacgacg ctggttggtg gtcgtgcccg acaagatcgc gcatcagcac
     3361 gtctaaaacg tagtcgtcga ctggaatcgt ttctcggaag cgaggcatgt tcggaaagat
     3421 gagggatgaa ggaggaagga agaaaggttc tgtttaacag ctataccgat taacggacag
     3481 cactttctaa cgtcgcgagc ggctcgcacc tggacgcaaa tgcggccgcg gatttatgtt
     3541 cggcgcacca tgatcaaaca aatcaaattc gttagcatcc cagtgcgcga tcagaaccgg
     3601 gcgctggatt tttacaccga gaaactcggg ttcaccatca tcaccgatca gccgttcgac
     3661 gacaaacaac gctggatcga actgcgcgtg ccgaaggcgg agacacgggt ggtgttgttc
     3721 ac
//
LOCUS       DASQUQ010000188         4988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_699082, whole genome shotgun sequence.
ACCESSION   DASQUQ010000188 DASQUQ010000000
VERSION     DASQUQ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699082"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VE486_05350"
     CDS             <1..392
                     /locus_tag="VE486_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38545.1"
                     /translation="PSRLLKGGSARILRAKINWRFTMAEENKAEQKASAAADAQEKLE
                     SSKQHARKAAEDLKSAAGAIAGEYRGKAEQAWGDARGKAEQAWGDATERVRTFQEDAE
                     QYVRENPTKAVFTALGIGFVLGLIFRR"
     gene            408..839
                     /locus_tag="VE486_05355"
     CDS             408..839
                     /locus_tag="VE486_05355"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZA38546.1"
                     /translation="MISETPRSRNPAGHTGLLANLLALASALAEFFESRFALVAQESK
                     AALVQVLVLAACLILALFLFVLGYVFLISSAIVGVAHLAGISWVWIALAAAGVHFLIA
                     LVLLLVARSRITKPVFRATLAELKKDREWLKNLDRTDRLTS"
     gene            797..1189
                     /locus_tag="VE486_05360"
     CDS             797..1189
                     /locus_tag="VE486_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38547.1"
                     /translation="MAEKPGQDRSIDELRAEIGSSRERVARDLRGLRYELDVPRKIRR
                     SFREQTGSWIAATAAVGTLVVLLPLRKKKIYIDAKSGRKSTKKLAEAGFALGALKIAA
                     SLVRPVVVEFVKNRLIGVAGQSRRSRRQ"
     gene            1246..2349
                     /locus_tag="VE486_05365"
     CDS             1246..2349
                     /locus_tag="VE486_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZA38548.1"
                     /translation="MIAEAPDIPVKSHPLGHVDEYLEIGQKAGASDVHLAVNAQPRWR
                     LHGTLGPIWPDAPRLTAEDTVALAERFVPDVYKEELNARGDSDFAYANEFARYRTSVV
                     RQRLGLEIVFRVINSQVRTMDELELPEHLKLLTRYQNGLILVTGSVGTGKSTTLAAMI
                     EQINMERRDHIITLEDPIEYIIKSRNCHVSQREIFTHTESFGSALRASLREDPDVIMV
                     GEMRDLETISLAITASETGHLVLATLHTGNASRTLDRLLDVFPSDQQEQIRVMVSESL
                     RGVISHQLIPKKDGTGRVLALEILTNTPAVANVIREAKTYMLPGIIQTGKKQGMRLMD
                     DTLMELYDRGLISGEEAYARADQKQMVRLHLKM"
     gene            2393..3559
                     /locus_tag="VE486_05370"
     CDS             2393..3559
                     /locus_tag="VE486_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZA38549.1"
                     /translation="MAYLDQFLSVIVKHGGSDLHIGERQPPKMRLHGDIMPIRADPVT
                     HEEAARMMSEICGPHNWEIFEQRGDLDFAYEMDEASRFRCNYFKQSNGYGAVFRLIPT
                     KIATLEQLGIPVVVKEFAHLRGGLVLVTGPTGSGKTTTQAALIDYINQNFAKHVVTIE
                     EPIEFVHDNKRSTITQREVPGNSSTFAAGLKAALREDTDIVLVGEMRDLETVSLALTA
                     AETGLLVFGTLHTNNARKTVDRMVDVFPADRQPQARAMLANSLRGVVAQLLLKRLDRP
                     GRIAVNEILIANAAVAAIIREGATQKLQDVIVSGKAQGMQFMDDAIWALLEKGIVSSH
                     EAFMKAIDKNRFKPFLSAEEEALANAAGAAPDDAKRLPGNFIKPFKASARARAG"
     gene            complement(3653..4642)
                     /locus_tag="VE486_05375"
     CDS             complement(3653..4642)
                     /locus_tag="VE486_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZA38550.1"
                     /translation="MVLEKPRQPLQLRDGPKPKPAAGQLLVRVATCAVCRTDLHVVDG
                     ELPDPKLPLIPGHQIVGRVEEIGGDVEGFFVGDRVGIPWLGWTDGECRYCRSNRENLC
                     DRARFTGYTIDGGYAEFTVADARFCFRLPNHYNDVDVAPLLCAGLIGYRSLRKTGDAR
                     RLGIYGFGNAAHLIAQVALYQGRELFAFTRPGDKATQQAAREVGAKWAGGSDEIPPEK
                     LDAAIVFASVGPLVPVALRALAKGGVVVCGGIHMSDIPSFPYADLWGERAITSVANLT
                     RRDGEEFLQVAPRVPVKTKTQTFALEEANSALEKFRAGELTATAVLRISEVSA"
     gene            complement(4806..>4988)
                     /locus_tag="VE486_05380"
     CDS             complement(4806..>4988)
                     /locus_tag="VE486_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38551.1"
                     /translation="FSQLVTLFVTPVIYLWLEWFQEHVLDKVPFLRSAHMHHEGKLVP
                     HPAGTGDGELEPAGVP"
BASE COUNT         1140 a         1375 c         1437 g         1036 t
ORIGIN      
        1 ggccgtcgcg tttgctgaaa ggcggaagcg cgcggatact gagggcgaaa atcaactgga
       61 gatttacaat ggctgaagag aacaaagcag aacaaaaagc gtccgccgcg gcggacgcgc
      121 aggaaaaatt ggaaagcagc aaacaacatg cgcgtaaagc ggctgaggat ttgaaatccg
      181 cggcaggcgc catcgcggga gaataccgcg gcaaggcgga gcaagcctgg ggcgatgcgc
      241 gtggcaaggc agaacaagcc tggggcgatg cgacagagcg cgtccgcaca tttcaagaag
      301 acgccgagca atacgtgcgc gaaaacccga ccaaggcggt gtttaccgcg ctcggaatcg
      361 gttttgtgct gggcctgatc ttccggcgtt agtcgcgcag ctaccgaatg atcagcgaga
      421 cgcctcggtc ccgcaatccg gcggggcata ccggtttgct ggcaaatttg ctggcgctgg
      481 caagtgcgct cgcagaattt ttcgaaagcc gctttgcgct tgtcgctcag gaatcgaaag
      541 cggcgctggt gcaggtgctc gtcctcgcgg cttgcctgat tctcgctctg ttccttttcg
      601 tccttggata cgtttttctt atctccagcg ccattgttgg cgtggcgcat cttgctggga
      661 tttcatgggt atggatcgcg ctagccgctg ctggagtaca ttttcttatt gcgttagtgc
      721 tgcttctggt cgcacgtagc cgaataacca aaccggtctt tcgcgcgacg ctagctgaat
      781 tgaagaaaga tcgcgaatgg ctgaaaaacc tggacaggac agatcgattg acgagctaag
      841 agcagaaatc ggcagttcac gtgaacgggt ggcgcgcgac ttgcgcggtt tgcgttatga
      901 attggatgtt ccgcggaaaa ttcggcgctc gttccgggaa caaacaggtt cctggattgc
      961 ggcgacggcg gccgttggca cccttgtcgt ccttctgccc ctccggaaaa agaagattta
     1021 cattgatgca aagagcggcc gcaaatcgac gaagaagctt gccgaagccg gattcgcctt
     1081 gggtgcgctg aaaatcgcag cgagtctggt tcggccggtc gttgtcgagt ttgtcaaaaa
     1141 ccggctgatc ggtgttgccg gccaatctcg ccgatcacga aggcagtgag cttgttttag
     1201 gattgcgccc ggcgcctgca tcgcataatc acctcgaagc aggcaatgat cgcagaggct
     1261 ccggacattc cagtcaaatc gcatccgctc gggcacgtgg atgaatacct cgagatcggg
     1321 caaaaagccg gcgcgtctga tgtccatctg gctgtaaacg cacagccgag atggcgtttg
     1381 catggaaccc tcggaccgat ctggcccgac gcaccgcgtt taacagccga ggatacggtg
     1441 gcattggccg aacgttttgt tcccgatgtg tataaagagg aactcaatgc gcgcggagat
     1501 tccgattttg cgtatgcaaa cgagttcgct cgctaccgca ccagtgtggt ccggcagcgg
     1561 ctcggcctcg aaattgtttt ccgcgtgatc aacagccagg tgcggacgat ggacgaactg
     1621 gagttgccgg agcatctcaa gttactcacc cgctatcaga acgggctgat tttagtgaca
     1681 ggctcggtgg gcacgggcaa atcgacaacg ctcgcggcga tgatcgagca aataaacatg
     1741 gagcggcgcg atcacatcat cactctcgag gatccgatcg aatacatcat caaatcgaga
     1801 aactgccacg tctcgcaacg ggaaatattc acgcataccg aatctttcgg ctcggcgctg
     1861 cgcgcttcgc tccgagaaga tccagatgtg atcatggttg gcgaaatgcg cgacttggaa
     1921 acaatttcgc tcgccatcac cgcgtccgag accggccatc ttgttttggc gacactgcac
     1981 accggcaatg cgtcgcgcac gctcgatcga ctactcgatg tctttccatc cgatcaacag
     2041 gaacaaatcc gggtgatggt gagcgaatcc ttgcgcggcg tcatcagtca ccagttgata
     2101 ccgaaaaagg atggcaccgg tcgcgtcctg gcgctagaaa ttcttacgaa taccccggca
     2161 gtggccaacg ttattcgcga agcaaaaaca tacatgttgc ccggtattat tcagaccggt
     2221 aaaaagcaag ggatgcgttt gatggacgat acgctgatgg agctttacga ccgcggcctg
     2281 atcagcgggg aagaagccta cgcgcgcgcc gaccaaaaac agatggtgcg gctgcatctt
     2341 aaaatgtgat ctccaacttc ttacttcttc tttcttactt atcgctgctc agatggctta
     2401 cctggatcaa ttcctaagtg tgatcgtcaa acacggcggg tccgatctgc acatcggtga
     2461 aaggcagccg ccgaaaatgc gcttgcacgg cgatattatg cccatccgcg ctgatccggt
     2521 gacgcacgag gaggcggcgc gaatgatgag tgaaatttgc ggcccgcata actgggaaat
     2581 tttcgagcaa cgcggcgatc tcgattttgc ctacgagatg gatgaggcct ctcgctttcg
     2641 ctgcaactat ttcaagcagt cgaatggtta cggcgcggtt tttcgtctga ttccgaccaa
     2701 gattgccaca ctggagcaat tgggaattcc cgtggttgtg aaggaatttg cgcacttgcg
     2761 cggcggcctt gtcctggtaa ccggcccaac cggttccggt aaaacaacga cccaggccgc
     2821 gctcatcgac tatatcaacc aaaactttgc caagcacgtc gtgacaattg aagaaccgat
     2881 cgaattcgtg cacgacaaca agcgaagcac aatcacgcaa cgggaagtgc ctgggaattc
     2941 gagtacgttt gccgccggtc tcaaggcggc actgcgcgag gacacggaca tcgtgctggt
     3001 gggagaaatg cgcgacctcg agacggtctc gctcgcgctg actgcggccg agaccggtct
     3061 tctcgtgttc ggcacgttgc acacaaacaa cgcgcgcaag acggtcgatc gcatggtgga
     3121 tgttttcccg gccgaccggc agccgcaggc gcgcgcgatg ctcgcgaact cactccgcgg
     3181 tgtagttgca caattgcttt tgaaaagact ggaccggccg ggcagaattg cggtgaacga
     3241 aattctgatc gcaaatgccg ctgtcgccgc catcattcgc gaaggcgcga cccaaaaact
     3301 ccaggacgtg attgtctctg gcaaagcgca gggtatgcaa ttcatggacg acgccatctg
     3361 ggcgttgctt gaaaaaggga tcgtctcttc gcacgaagcc ttcatgaaag cgatcgataa
     3421 aaatcgcttc aaaccatttc tctccgccga ggaagaagcg ctcgccaacg ccgctggtgc
     3481 agctccggac gatgcgaaac ggttgcccgg caattttata aaaccattca aggctagcgc
     3541 gcgcgcccgg gcgggttgag gcattcgctt ttggcagcgc gaagatcgcc gttttgtagc
     3601 tggcgctaca acaagtaagt gcaggcggcg cacagtaaca ttcgcgtcag gcttacgcac
     3661 taacttcaga gattcttagc acggcagtgg cggtgagctc accagcccgg aatttctcga
     3721 gcgcggaatt cgcttcctcg agcgcgaagg tttgcgtttt cgtttttacc ggaacgcgcg
     3781 gtgcgacctg gagaaattct tcgccgtcac ggcgcgtcag atttgctact gaagtgatcg
     3841 cccgttcgcc ccaaagatcc gcatacggaa atgacgggat gtcgctcatg tgaatcccgc
     3901 cgcaaacgac aacaccgccc ttggcgagcg cgcgtagcgc gacgggcacg agcggcccga
     3961 cagacgcgaa tacgatggcg gcatcgagtt tctccggtgg gatttcatca gagccgccag
     4021 cccatttcgc gcccacctct ctcgctgctt gctgcgtcgc cttgtctcca gggcgagtga
     4081 acgcgaaaag ttcgcgacct tggtagagcg cgacctgcgc gatcaaatgc gccgcgttac
     4141 cgaatccgta aatgccaaga cggcgcgcat cgccggtctt acgcaatgac cgataaccga
     4201 tcaaccccgc gcaaagcaaa ggtgcaacat cgacatcgtt gtagtggtta ggcagacgaa
     4261 aacagaaccg ggcatcggcg acggtgaatt cagcgtatcc cccgtcgatt gtgtagccgg
     4321 tgaaacgcgc gcgatcacaa agattttccc ggttcgatcg acaatagcgg cattcgccat
     4381 cagtccagcc cagccatgga atacccacgc ggtcgccgac gaaaaatcct tcaacatcgc
     4441 cgccgatttc ctcaacacgc ccgacgattt ggtgcccggg aatcagcggt agtttgggat
     4501 ccggtaattc gccgtcgacg acatggagat ctgtccgaca aacagcgcat gtggcgactc
     4561 gcactagcag ttgtccggca gccggcttcg gtttcggccc atcgcggagt tgcagcggct
     4621 gccgtggttt ctccagcacc attgcgcgca tgctataagc attaaatgac gaatgacgaa
     4681 tatcgaatgt cgaaggaaga ccgaatgctc ggaatgacaa aagagtgatc gaacacttcg
     4741 tatcgccgtt cggatttcgt cattccttcg agcttcgtca ttcgtcattt tttccatcgt
     4801 catgatcagg gcacgcctgc tggttccaac tcgccgtcgc cggttccggc gggatgcggg
     4861 acgagtttac cctcatgatg catgtgcgcg ctgcgcagaa atggcacttt gtcgagcacg
     4921 tgctcctgga accattcgag ccagagataa atgaccgggg tcacaaaaag cgtgaccaat
     4981 tgcgagaa
//
LOCUS       DASQUQ010000189         4753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_583445, whole genome shotgun sequence.
ACCESSION   DASQUQ010000189 DASQUQ010000000
VERSION     DASQUQ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583445"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VE486_05385"
     CDS             <1..1084
                     /locus_tag="VE486_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38552.1"
                     /translation="FFEVRKRLALIVIGVGNIGGTLLRQLHEQRAYLLARGFDVTVIG
                     VANSKWFALEGNGINLARWRERLSASRRRMDPQVLAREIAKLELTDAAVVDCTAADSI
                     VNAYPDFVRANLHIITPNKRANVLPWRQYAALMELLRKRQKYFLDEANVGAGLPIMST
                     LRDLIASGDVIRKVEGIFSGTLSYLFNSFDGSMPFSALVRTAHQTGLTEPDPRDDLSG
                     QDVARKLLILARQSGSKLEIADVQVESLVPRSLALRRFSHRFFSSLAKHDCEMAERLK
                     RAQSRGAVLRYVGTLEGGRARAGIREFPHDHPIAATKGSDNIIAFTTKRYSRTPLVVQ
                     GPGAGADVTAMGVFSDILKLLNYLPH"
     gene            complement(1158..1469)
                     /locus_tag="VE486_05390"
     CDS             complement(1158..1469)
                     /locus_tag="VE486_05390"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HZA38553.1"
                     /translation="MIDAPAREAHEAREEIKKQAYAEIAKARFAIDDTDKKSNLDLAT
                     HFNFVSDADITGGNSGSPVVNKAGEFVGIIFDGNIRSLVLDCVYTDKEARRVGRFRGD
                     H"
     gene            complement(1466..1642)
                     /locus_tag="VE486_05395"
     CDS             complement(1466..1642)
                     /locus_tag="VE486_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38554.1"
                     /translation="MSIFAGKFGTNDPLVKQVLAGKSPHGRAVELVPGTELKDVAVRK
                     ELYTKKRGGSISLV"
     gene            1937..2698
                     /locus_tag="VE486_05400"
     CDS             1937..2698
                     /locus_tag="VE486_05400"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZA38555.1"
                     /translation="MFKLQRRKIERLDFILAGAQKSGTTALHYFLSKHPNITMGDQQE
                     MHFFDDDAMFVSQADYEQLHKHYRPVAPSTIAGDCTPSYMYHAPAAERIWKYNPKIKL
                     LILLRNPVERAFAHWNMQRFKAREPLDFFDAVREEQTRIAGAPPAEARRFAYIDRGFY
                     GRQLARLFKFFPREQVKVVKFEQFKTNQRETLASILSFLGCKPLRSVRSKDRNIVPYE
                     RAMNWEERVFLYNLFAEDIAQVERMLGWDCSGWKL"
     gene            complement(2712..3311)
                     /locus_tag="VE486_05405"
     CDS             complement(2712..3311)
                     /locus_tag="VE486_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38556.1"
                     /translation="MRKFLITVAILVGCFAAVSAIWIFGGRQVSLFIDRYRTIETASE
                     RVNSISYEGSGTGGILRVNDLALSLNDTNGPSPNIGSTKDDQLAVAAGGKVFAFGPPR
                     SEAENLVASPPADDDASVTIQHSILSWPTSFDFNFMTGQSPSWKRHIYYRLLWKKPSG
                     AALQMLWRYEQYFYPANGWGSGFMTREGSTGLVEVDIRP"
     gene            complement(3446..4531)
                     /locus_tag="VE486_05410"
     CDS             complement(3446..4531)
                     /locus_tag="VE486_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38557.1"
                     /translation="MAKRVCYIHIGPHKTGTSSIQWFLKENRAELLKHGYFVPESGAN
                     HGAHHAIVRKLCGQELRDHQQSAAADFARALDETPSEAVVISSEALHPLLKRSDLARI
                     FFTRTRELNLEPKLVLFPRNQSQFINSCYSQAVKGFRRSEPFEAFVQGATRRFSFRYS
                     PLIELAKIYDVELVPRPFTGETIARGVVPEFLKAIGLDPSQFRDTNVRRNEAAGPFTV
                     GVARGVLRSISSTGKRLKWLQAVRCGTKLATYLKDKGLADAGYCGLTTTLARHIETQW
                     RSDNDAFAQRVWGAPWSEIFAADIGREFTPNDFAMCRPDETTERRLQQAIGEMTPIAE
                     RVLLEPALAVEAPWNDLLGRGGWTPRE"
BASE COUNT         1037 a         1327 c         1343 g         1046 t
ORIGIN      
        1 gttcttcgaa gtccgcaaac gcctcgcgct gatcgtaatc ggcgtcggaa atatcggtgg
       61 aacgctactg cgacaactgc acgagcaacg cgcgtatctg cttgcccgtg gattcgatgt
      121 cacggtgatt ggcgtggcca atagcaagtg gtttgcgctc gagggtaacg gcatcaatct
      181 cgcacgctgg cgcgagagat tatccgcttc gcgaagacgg atggatccgc aggtgctggc
      241 aagggaaatc gcaaaactcg agttaaccga cgcagcggtg gttgattgca cggccgccga
      301 ttcaatcgtg aacgcatatc ccgattttgt cagagcgaat ctgcacatca tcacgccgaa
      361 caagcgcgcg aacgtgttgc cgtggcggca gtacgcggcc ctcatggagc ttttgcgaaa
      421 gcgacagaag tactttctgg atgaagccaa cgttggcgcg ggactgccta tcatgtcgac
      481 gctgcgcgac ctgatcgcca gcggagacgt gatcaggaag gtggaaggca ttttctcggg
      541 cacgctgagc tatctgttca atagtttcga tgggagcatg ccatttagcg ctctcgtgcg
      601 aacagcgcac cagacggggc tgaccgagcc ggatccgcgc gatgatctgt ccggccagga
      661 cgtcgccagg aagttgctta ttctcgcgcg tcaaagtgga tcgaaattgg aaatcgcgga
      721 cgtccaggtt gagagcctgg ttcctcgaag tttggcgctg aggcgattct cgcaccgatt
      781 tttcagctca ttagctaagc acgattgcga aatggctgaa cgattgaaac gtgcgcaatc
      841 gcgcggcgca gtgctgcgat acgtcggcac gctcgaaggc ggtcgcgcgc gcgccggaat
      901 cagagagttc ccgcacgatc atccaatcgc cgctactaaa ggcagcgaca acatcatcgc
      961 atttacgacg aagcgttatt cgcgtacccc tctcgttgtt cagggcccag gggcgggcgc
     1021 ggatgttacg gcgatgggcg ttttttccga tatcctcaag ttgctcaatt acctgccgca
     1081 ctaggatcca gaattatttt ttcacgcctt cgagtttatc cgggaggtcg cccgcatcat
     1141 aaactttgcg cagcgcttca atgatcgccg cggaatcgac cgacacggcg cgcttctttg
     1201 tcggtgtaga cgcaatcgag cacgagcgat cgaatgttgc cgtcgaaaat gattcccaca
     1261 aactcgccgg ctttgtttac aacagggctg ccgctgttgc cgccggtgat gtccgcgtcg
     1321 gagacgaaat tgaaatgagt cgccaaatct aaattcgact tcttgtcggt gtcatcaatg
     1381 gcaaaacgcg ctttcgcgat ctcggcatac gcctgtttct tgatttcctc ccgggcttcg
     1441 tgtgcctcgc gcgcaggtgc gtcgatcata cgagcgagat cgagccgcca cgttttttcg
     1501 tgtaaagctc tttccgcact gcaacatctt tcaactcagt gccgggaacc aattctacgg
     1561 cgcgcccatg cggcgatttg ccggctaaca cttgcttcac gagcgggtcg ttggtgccga
     1621 acttcccagc gaagatcgac aatcaccttg tcgccacgcg ccgagtgctg gggagcacac
     1681 gctgccagcg tgttggtctc ggcaacttgc cgagactttc ttatgaaatt cgtgccaagc
     1741 gcgggaagcc tgttgccggc aactgcagac tggcagcctg cgctccccaa actcgtggag
     1801 tgctacgaca ggcctgcggc aagcgaacag gaaacctatg ttccgtagcg ctaagcgcgc
     1861 gcacgtgcga cgatcccgtg gccacgacgc tatgcgccgt gctggaagcg gccacaggcc
     1921 gctggctaca tgtcctatgt ttaagctgca gcggcgaaag atcgagcggc tcgatttcat
     1981 tctggccggc gcacagaaat ccggcacaac ggcgcttcat tattttttgt cgaagcatcc
     2041 gaacatcacc atgggcgacc agcaggagat gcatttcttc gatgacgacg cgatgtttgt
     2101 ctcgcaggcc gattatgagc aattacacaa acattatcgg ccggtcgctc cctcaaccat
     2161 cgccggtgat tgcacgccta gttacatgta tcacgcgccg gcggcagaac gcatctggaa
     2221 atacaacccg aaaatcaaat tgctcattct cttgcgcaat cccgtcgagc gcgcctttgc
     2281 gcattggaac atgcaacgat ttaaagcccg cgaaccgctg gattttttcg atgccgtgcg
     2341 agaagagcaa acgcgcatcg ccggtgcgcc gccggctgaa gcgcggcgct ttgcttacat
     2401 cgatcgggga ttctatggac ggcaactcgc ccgtctcttc aaattctttc cgcgcgagca
     2461 agtgaaagtg gtaaagttcg aacagttcaa gacgaaccag cgcgaaacgc tcgcgtcaat
     2521 cttatcgttc cttggctgca agccgctgcg ttcggtgcgc agcaaagaca gaaatattgt
     2581 gccttacgag cgcgcgatga actgggaaga aagggttttc ctgtataatc tgttcgcgga
     2641 agacatcgca caggttgagc gaatgctggg ctgggattgc tctggctgga agttgtgaga
     2701 tttcagccgc gttaagggcg aatatcaact tcgactaggc ccgtggatcc ttcccttgtc
     2761 atgaatccgc ttccccagcc gttggcgggg tagaagtatt gctcgtagcg ccaaagcatt
     2821 tgcaacgccg cgccggaagg ttttttccaa agaagccggt agtagatgtg ccgtttccat
     2881 gatggcgact ggccagtcat gaagttgaaa tcgaatgaag tcggccagct aagaatgctg
     2941 tgctgaattg tgaccgaagc gtcatcgtct gctgggggcg aagcaacgag attttccgct
     3001 tcggatcgcg gtggaccgaa agcgaaaacc tttccaccgg ctgccacggc aagctgatca
     3061 tcttttgtcg agccgatgtt aggactcgga ccattcgtgt cgttcagact tagcgcgaga
     3121 tcgttgacgc ggagaatgcc accagttccg ctgccttcgt agctgattga gttaacacgc
     3181 tctgaagctg tttcaatcgt cctgtaccga tcgataaaca atgagacctg acgcccgcca
     3241 aagatccaga tcgccgagac tgccgcgaag catccgacaa ggatcgcaac tgtgatcagg
     3301 aattttcgca caacccaaaa cctgtgagct acagatgaac acaaatcgac acagatttct
     3361 atctatgttt catcgacggg atagcgccgc atgccgacca aaattgtccc atgcttgagc
     3421 acagccccga gaaagtcata ggaatctact ctcttggagt ccagccaccc cgtccaagca
     3481 agtcgttcca aggagcttcg acggcgagcg ccggttcgag cagaacccgt tccgcaattg
     3541 gggtcatttc gccaatagct tgctggagcc ggcgctcggt ggtctcgtcc ggccggcaca
     3601 tcgcgaaatc gttgggcgta aactcgcggc caatatcggc ggcgaaaatc tcgctccacg
     3661 gtgcgcccca gactcgttgc gcgaatgcat cattatctga tcgccactgt gtttcgatgt
     3721 gccgcgccag ggtcgtggtc agtccgcaat atccggcatc ggccagtcct ttgtccttaa
     3781 ggtatgtagc aagtttagtc ccgcatcgca cggcttgcag ccacttgagt cgcttgcccg
     3841 tgctgctgat cgagcgcaat acaccgcgtg cgacgccaac ggtaaatggt ccggccgctt
     3901 cgttacggcg cacattcgta tcccggaact gcgacgggtc gagaccaatg gctttgagaa
     3961 actctggcac gacaccgcgg gcaatagttt ctccagtgaa tggacgggga acgagttcga
     4021 catcgtagat cttagccaac tcgatcaagg gcgagtatct aaaactaaag cgccgagtgg
     4081 ctccttgaac gaaagcctcg aatggttcgg atcggcggaa gcctttcacc gcctgcgaat
     4141 agcaggaatt aataaattgc gattggttgc gtgggaacaa tacgagcttc ggttcgaggt
     4201 tgagttctcg ggtccgggtg aagaaaatcc tggcaaggtc gctccttttt aaaagcggat
     4261 gcagggcctc cgaggaaata acgactgcct cggacggcgt ctcatcgagc gcccgggcaa
     4321 aatcagcggc cgccgactgt tggtgatccc gcagctcctg tccgcagagt ttccgaacga
     4381 tcgcatggtg cgcgccgtga ttggccccgc tttccggcac gaaatagcca tgcttcagca
     4441 actcggctcg gttttccttc aggaaccact ggatcgagct cgtgcccgtt ttgtgcggac
     4501 caatgtggat gtagcagacg cgctttgcca tccgggaact atagtccgga gcgcctgccg
     4561 gtggcaattc acgccgcctc tagattgaga gttgtttcgg cgctcacagt caaatcagtt
     4621 cggaagattt tctgcgggcg agcgatgacc ggatcgaaac gaccgctcgt tccaaggggc
     4681 ttcgatagca agcgccggat cgcgcaagat ctcttccgca attgccgtca tctcgtgaat
     4741 agcccgcccg agt
//
LOCUS       DASQUQ010000190        12886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_515688, whole genome shotgun sequence.
ACCESSION   DASQUQ010000190 DASQUQ010000000
VERSION     DASQUQ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515688"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VE486_05415"
     CDS             <1..1001
                     /locus_tag="VE486_05415"
                     /inference="COORDINATES: protein motif:HMM:NF036494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZA38558.1"
                     /translation="QHLAIQISAQINPGNSGGPVMQNGKVVGVAFQGYSGDVAQGVAY
                     MIPTPVINRFLKDIGNGHYDKYVDLGMVVSNLQNQAQRRFLGLKDDDRGVLVSTVVAA
                     GPSANILHPGDVLLTIDDHPIASDKNVELEGERADFQEVVERKFNGDSVKFDIWRDKQ
                     PMTVTIKLFTPWPYRIQGHSYDVRPRFVLYGGLLFQPLNLDLLEAYRSTDLRLRHFFE
                     YFVLEQIYLEHPDVIVLTNILPDPMNTYLAPYRGGIVDEINGKKIRTLDELASAFTET
                     PERFVIRMIGDGPPLVLDRNKVEAARERIKTRYNVLKEQNLEEQPASEIAQQVKSN"
     gene            1001..2500
                     /locus_tag="VE486_05420"
     CDS             1001..2500
                     /locus_tag="VE486_05420"
                     /inference="COORDINATES: protein motif:HMM:NF036494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZA38559.1"
                     /translation="MQRHFAIIFGVVLVTLTGALAKKESGVPAPMTTQKQLALVRVNV
                     TGQSYDYFRPWQKKAPFSKRALGAVLSKGRVLVTADLVANQNFVELERAETGEKTAAK
                     VQVIDYEANLALLEPTEKTFLDGITPLEITADTVVGDRLAAWQLEPTGALLATEGLVT
                     TVQVTEYPIDVGQFLTYRVSIPMQYRENSYTVPLVKNNKLAGLLLRYDSRSQLLDAIP
                     APIITHFLKEADSQNYRGFPSAGFSFFPTRDPELREFAGEKGKGGGVYVTNVEPNTPA
                     MKAGLHVGDIVTAVADREIDQNGNYVDSLHGKIEFTNLLTAHAYAGDAVPFRVQRNGK
                     PVQLNVTLEHRDAKDYVSPPYNFDEPPRYYVLGGLIFQELSRQYLKEWGPTWERSAPQ
                     RLVYFDRFQSELFPEGNRRVVILSQVLPADSTIGYDELAYLTVTKVNGKEIKSLGDLA
                     EAVKQPIDGFIKIETEEDPKQIELDAAQVAAEAPNLQENYGISSMQHLD"
     gene            complement(2497..3945)
                     /locus_tag="VE486_05425"
     CDS             complement(2497..3945)
                     /locus_tag="VE486_05425"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HZA38560.1"
                     /translation="MDRIVGIETEYGCLLNKEEPHVNSELWPAKVKNYLFRKAEAGTI
                     DLHYRDYEEPPGNGGFLLNGGRLYLDMGHIEYASPECLHLHDLVTYELAGDDLLQSSL
                     VALGADSHVSFIKNNVDHYTGATFGCHENYLMKREAQFTPPILGTLLTFLATRQIFTG
                     SGRVGQSNPLAFDFEPPQPEAQVNFQLSQRADHIVNDIYQWVQFNRAIINARDEPLAD
                     YRKYRRLHLLIGDSNMSPFATALKIGTTACVLSLLEHGRLPRDLVLADAVQSTRDISR
                     DTTQQWIVQLENGKSIGALDVQWEFHQLAQKYLGGSSAETNWLLESWGFVLDALKQNH
                     QALIGGVDWITKKWLLETFVEAEKLTWSDPWLQSIDLEYHNIDPVRGLFFGVTPRKRI
                     AEWNNSVRRPSATHTPPANTRASGRARAVAFFQSRSGEIPYVINWDSIACDSRDFLVM
                     GDPFQTYNEEVDRFLARPRPSGGPSDLRPLDC"
     gene            4182..4610
                     /locus_tag="VE486_05430"
     CDS             4182..4610
                     /locus_tag="VE486_05430"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HZA38561.1"
                     /translation="MATVALRSPSEKVGGLFYFGRMLDKIRLHARGELPADYHENLGK
                     GFDEKCVTFLRINYDQLVERVKQGGSDEEILHWCFKAGRQPSEDDIYVWNEFMRKRGW
                     NDEVSEILKRRKKEAGMSDRSDIETAFQFIDADEGRLPSR"
     gene            complement(4705..4989)
                     /locus_tag="VE486_05435"
     CDS             complement(4705..4989)
                     /locus_tag="VE486_05435"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZA38562.1"
                     /translation="MADLIKAGELKERMKKVPEWELEKKHIERTFEFDDFADAIDFVN
                     AVAEVAEEEEHHPDIDIRYNKVHLVLSTHSKGGLTELDFGLAERIDTLAE"
     gene            complement(5045..6187)
                     /locus_tag="VE486_05440"
     CDS             complement(5045..6187)
                     /locus_tag="VE486_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38563.1"
                     /translation="MTIFLGCGFAAKYREGGGNLSVPLQWMLGLRRLKLDAIWLELLP
                     TTNDARADQAKIANFQRRLRDHGLAGRYCLLHQKPASDTHDLDGMDCIGISKRALLDR
                     LAGPNALLNLSYSIHPPFLLQFERRVFCDLDPSEIFYWMTKMEMGQSYHHEFWTIGLN
                     VHGRDCRLPKSSLPWKTFYPLADTKLLHRQPRPRISKFTTIGQWYWGGAVEVDGAFPD
                     LSKKFAFEPYLDLPKRIPEASFELAMNIKADDPERARLNQRGWQVVDPHRVARTPGSY
                     RRYMARALAEFTAIKGVDVAWRTGWLSDRAAAFLALGRPVVTEDTGAAKYLPSENGFH
                     FVRGIEEAEIAIRQTLQEWPTLSRQARNCAVEVFDSTKNLGKILDL"
     gene            complement(6242..8215)
                     /locus_tag="VE486_05445"
     CDS             complement(6242..8215)
                     /locus_tag="VE486_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HZA38564.1"
                     /translation="MKFVSTGLWVPALLCAGSLGAVTSTEWRFRQAVDVPSSGLVRVN
                     LPAATLDAARLDLGDVRILGSAGNEVPYLIDQPMPRRESALRSQELRTALEPTATRIT
                     LITGTKSLLKGVTFETPPGVEFIKAVQVEGSHDGATWHQLATGKPIFTMAGGAANPSI
                     SFPDGVWEFLRLTIDDSRTAAVPFTGVLLQVAETDAPVEPVPVTIKTRDESLGVTRLS
                     VDPGATNLTVASLRIETPDPLFARPITIAVPELGNDGLREQTLRTGSIYRLDLNGKTE
                     SQVEIPIDMQIRSRELIVLIDNGDSPPLAINAVDARRRVTNLVFFAREPGRYQLLSGN
                     NECPAPRYDLSELGDQLKSAAAIEVRPAPLIASPDYKPPDNLAALPLAGANIDVAAWK
                     FRKRVQLSKPGVQQIELDPDVLARAAPDHRDLRIVTDDQQLPFLFERTSISRSLPLIP
                     TSASDPKKPRLSRWSLKLPQAGVPISRLTCATGSALFQREMRLWEEVKDERGDTFHRE
                     LGRATWKRVPGEATRELTIQLDVAPGSGALFLETDNGDNPPIELHDFREHYPVTRVVF
                     KAASDSTQPIRLYYGNPDAASPLYDVILIADQLLRAERTSATLGPQENVGSKTERIIQ
                     TLSGSARYTFWGVLAVVVVALLLLVSRLLPKTQ"
     gene            complement(8212..11241)
                     /locus_tag="VE486_05450"
     CDS             complement(8212..11241)
                     /locus_tag="VE486_05450"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZA38565.1"
                     /translation="MEAFALLLLIVIAACFVLPLVAIAKAASARRSVEDFKTRLRNLE
                     AELQTLKRAPGEPSAERPFAAERETAEREPFVAPIATPSEARPVSVPPPLPEEVAAVA
                     TSTPPPIPPQPPSGESAPTQPLLPAINWEQFMGAKLFAWIGGLALFLGVAFFVKYSFE
                     HNLIPPEVRVAIGFAVGLALIVGGLGLRRKENVVTAQTLCATGILILYAVTFACRSFY
                     HFAFFDFVPTFLLMTLITAVAFLLAVRMNAIVVAILGMAGGFLTPILLSTGQDNPLGL
                     FGYIALLDIGLLMVARRKEWSALPVLGAVGTVLMQVGWVGQFFLREHYFLGNKVLVPM
                     AIFLGFEFLFSAALVSTKRTSKSEGAFSVAAIGMGGAAIACGFYFLSFQAFGYRPALL
                     LSYLFLADIGLLAIVFMKEKLAAVSTVTGLAVFAFLAIWTGWYLTTQNLYVALAAFFV
                     FALLHSTASLVLQRTRRIEAQWWSHLFPALALVLVLMPIFSLSEVSFLVWPMVFCVDL
                     LAIFVAVATGALLPIFAVLLLTFVVIGGWIFRIPIELTGLPTSLFLLGCFAVFFLLAA
                     IWACRPLLASGPSGVMKTRGKLFGALTDPANFAIQLPTLSATLPFLLLIMVTLRLPLT
                     NPAPVFGMALMFVVLLLGMAKIFSLDLLSAVGLGSTLALEYAWHSAHFNPDHVLLALA
                     WYIGFAAVFTIFPFLFQRQFAGKTVPWAAAALAAPLHFFLVYDAIRTAYPNGMLGLVP
                     AAFAVPSLLGLIVLLKRTPAESPARNAHLALFGGAALFFITLIFPIQFDRQWITLGWA
                     LEGAALCWLFHRVPHPGLRLVGTALLITAFVRLALNPAVLSYHARSATPIFNWYLYTY
                     GIVSVCLFAGARLLASPRNLVLGRNAQPLLYALGTILAFLLLNIEIADYFNRTGMATL
                     TFQFSGNFARDMSYSIAWALFALLLLVIGIRKTVRPVRYASLGLLGVTILKLFLHDLS
                     QLDQLYRIAAFVVVAIIAIFASFLYQRFLGLSAKQTQ"
     gene            11336..12223
                     /locus_tag="VE486_05455"
     CDS             11336..12223
                     /locus_tag="VE486_05455"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZA38566.1"
                     /translation="MNIHHLELFYYVAKHGGIAAAVRKMPYGIQQPAVSGQIARLEEA
                     LGAKLFNRRPFSLLPAGAELFEFIRPFFDDVEKVAGRIRGASLQLRIAAPSVVLHDYV
                     PELLRRIRRRFPSFRLQLHEAVRADAERLLLAGEIDLAITILDGKRRPEIHSRPLLEL
                     PMILLVNKKQNITRAEQLWARDKIEETLITFPRGDAVQAHFQRGLQQLGVEWFPGIEV
                     NTTRLIEPYVTHGYGIGLTVAPPSFKSPPGVRAIPLPGFSPVIVGVAWAGKLSPIAQQ
                     FLAEVEIEADAVKRSSKCR"
     gene            complement(12262..>12886)
                     /locus_tag="VE486_05460"
     CDS             complement(12262..>12886)
                     /locus_tag="VE486_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38567.1"
                     /translation="VDTDTGDVFAYTDDEFTALLYEFRAQIAEYLATLTHTNLRTLAD
                     LIAFNNAHCEQELVFYGQEVFELSEQFPGYPNDPNYIAARTHARNTARSGIDDALAAD
                     DLDAIVAPHLTNSTGPAVSGYPNLSLPVGIRDNGRPAGMLMYSTFLHEPELIGFGFDL
                     EQELNVRRQPQFLGSVIPVPNAGLCTGEARPPQVFTGKAHIPYGRIF"
BASE COUNT         3111 a         3621 c         3567 g         2587 t
ORIGIN      
        1 atcagcacct tgcgattcag atcagtgcgc agatcaatcc gggcaatagc ggtggaccag
       61 tgatgcaaaa cggcaaggta gtcggcgttg catttcaagg ttacagcggc gacgtcgctc
      121 aaggcgtcgc ctacatgatc ccgactcccg tgattaaccg atttctaaag gacatcggca
      181 acggacatta cgacaagtat gtcgatctgg gcatggtcgt ttcaaacctc caaaaccaag
      241 cccagagaag atttctcgga ttgaaagatg acgatcgtgg cgtgctggta agcacagtgg
      301 tcgcagccgg gccatcggcc aacatccttc accctgggga tgttcttctc acgatcgatg
      361 atcatcccat cgcaagcgat aagaatgtcg aattggaagg tgagcgcgca gacttccagg
      421 aagtggtcga acggaagttc aacggcgatt cggtcaagtt cgacatctgg cgcgacaaac
      481 agcccatgac ggtcacgatc aaactcttta caccgtggcc ttatcgaatt cagggccaca
      541 gctacgatgt gcgtccccgc tttgtgcttt acggtggctt gctttttcag ccactgaacc
      601 tcgatttgct cgaagcatat cggtcaaccg atctgcgtct ccgccatttc ttcgaatact
      661 ttgttctgga acaaatttac ctggagcatc ctgacgtcat cgtgcttacc aatattttgc
      721 cggacccgat gaatacctac ctcgcgccgt accgcggcgg gattgtcgat gaaataaacg
      781 gcaaaaaaat tcgaaccctc gacgaactgg cgagcgcttt caccgaaaca ccggagcggt
      841 tcgtcattcg aatgattggg gacggccctc ctttggtgct cgatcgcaac aaagtagaag
      901 ccgcacggga gcggatcaag acgcgttata acgtgttgaa agagcagaat ctcgaggagc
      961 aacccgcgtc agaaatagca caacaggtga agagcaactg atgcaaagac atttcgctat
     1021 tattttcgga gtggttcttg tcacgctgac cggagcgctg gccaaaaagg aatccggcgt
     1081 tccagcgcca atgaccacac aaaagcagtt ggcgctcgtc cgcgtgaatg tgaccgggca
     1141 gtcgtacgat tattttcgac cctggcaaaa gaaagcgccc ttttcaaagc gcgccttggg
     1201 cgctgtttta tcgaaaggcc gtgtcctggt aaccgcagat ctggttgcaa accaaaattt
     1261 cgtcgaactc gagcgcgcgg agaccgggga aaaaacggcc gccaaggtcc aggtgattga
     1321 ttatgaagcc aacctggcgc tgctcgagcc aacagagaaa acattcctcg atggaatcac
     1381 cccgctcgag atcacggccg acacggtcgt tggcgatcgg cttgccgcgt ggcaactcga
     1441 accgactggc gcattgcttg cgaccgaagg cctggtaacg accgtgcaag tgaccgaata
     1501 tccaattgat gttggccaat ttcttaccta cagggtcagc atcccgatgc agtatcgaga
     1561 gaacagctac acagtgccgc tggtgaagaa taacaagctc gccggattgc tgctccgcta
     1621 cgattcacgt tcgcaactcc tcgacgccat ccccgcgccc atcatcacgc attttttaaa
     1681 ggaagctgat agccagaatt atcgcggttt cccgtctgct ggcttctcat ttttcccaac
     1741 acgcgacccg gagctgcgtg agtttgccgg agaaaagggc aagggcggcg gcgtttacgt
     1801 tacgaatgtg gaaccgaata cgccggcaat gaaggccggc ctgcacgtcg gcgacatcgt
     1861 gacagcggtt gcagaccgcg aaatcgatca gaatggcaac tacgtagatt cgctccacgg
     1921 gaagatcgag ttcacaaatt tgcttactgc gcatgcgtac gccggcgatg ctgtcccctt
     1981 ccgcgttcag cgtaacggca agcctgtcca gttaaatgtg acgctcgagc atcgggacgc
     2041 gaaagattat gtcagcccgc cctacaattt cgatgaacct ccacgctatt atgtcctcgg
     2101 gggcctcatc tttcaggagc tttcccgcca gtatctaaaa gaatggggcc caacctggga
     2161 aagatcagcc ccccagcgtc tggtttactt tgatcgtttc caatcggagc tctttccaga
     2221 gggaaatcgc cgcgtcgtca tcctcagtca ggttctcccg gccgacagca caatcggcta
     2281 cgatgagctc gcctatttga cggtcacaaa agtaaacgga aaagaaatta aatcgttagg
     2341 cgatctggcg gaagcggtga agcagccaat tgacggcttt atcaaaatcg agacggaaga
     2401 ggatccaaaa cagattgagc tcgacgccgc gcaagtggcg gccgaagcgc ccaatctcca
     2461 ggaaaattac ggcatctcct cgatgcaaca cctcgactag caatccagtg gtcggaggtc
     2521 agagggaccg ccagatggcc gcggccttgc taaaaaccgg tccacttcct cgttgtaagt
     2581 ttggaacggg tcgcccatga cgagaaaatc gcggctgtcg cacgcgatcg aatcccagtt
     2641 aatcacgtac gggatctcgc cactgcgaga ctggaaaaaa gccactgcac gcgcgcgacc
     2701 tgatgcacgc gtattggcag gcggggtgtg tgtcgcgctc gggcgacgga cactgttgtt
     2761 ccattctgca attcgcttgc gcggagtgac gccgaaaaat aatccccgga ccggatcgat
     2821 gttgtgatat tcgagatcga tgctttgcag ccatggatca ctccaggtca atttttccgc
     2881 ctccacaaat gtttcgagca accatttctt tgtgatccag tcgaccccgc ctatcagcgc
     2941 ttgatggttt tgcttgagag cgtcaaggac gaagccccag ctttccagca gccagtttgt
     3001 ttccgcgctg ctgccgccca ggtatttctg tgccagttga tggaattccc actgcacatc
     3061 gagcgcgccg atgctcttgc cattctcaag ctggacaatc cattgctgag tagtatcgcg
     3121 cgagatatca cgcgtgctct gaacggcatc ggccaggaca aggtcccgcg gcaaacggcc
     3181 atgctccagc agcgacaaaa cgcaagcagt cgtcccaatc tttagcgccg tggcaaacgg
     3241 gctcatgtta gagtcgccga tgagcaggtg aagccgccga tattttcggt aatcagccag
     3301 cggctcatcc cgggcattga tgatggcgcg attgaattgc acccactgat agatgtcgtt
     3361 cacgatgtga tcggcgcgct ggctaagctg aaaattgacc tgcgcctccg gctgcggcgg
     3421 ctcaaaatcg aacgccagcg gattggactg accgacccga cccgacccgg taaatatctg
     3481 ccgcgtcgcc aaaaaagtaa gcagcgttcc gagaattggc ggggtgaact gcgcctcccg
     3541 tttcatcaaa taattctcat ggcaaccaaa cgtggccccc gtgtaatgat cgacattgtt
     3601 tttgatgaac gacacgtgac tgtctgcgcc cagtgcgacg agtgacgatt ggagcaggtc
     3661 gtctcctgcc agctcgtagg tgacaagatc gtgcaaatgc aagcactccg gcgacgcgta
     3721 ctcgatgtgt cccatgtcga gatagagacg cccgccattg aggagaaaac caccgttgcc
     3781 cggtggttcc tcgtaatcgc ggtaatgcag atcgatcgtc cccgcctccg ctttacgaaa
     3841 gaggtaattc ttgacctttg ccggccacag ctcggaattg acgtgcggct cttctttatt
     3901 cagaagacag ccgtattcgg tttcaattcc aacgatccga tccatcgaat taattaacgc
     3961 accgaatcgc gaaatcgcta agcgaaaaac ttagcctccc gccggcaaat aacgagcgcc
     4021 agctgctgta gtgtccgctg tcctcggcgg acaattcgcg agccacggcg cataagccag
     4081 tgcgctgggg acggcgcacg ctacagcacg tttggacgga aggtgaggcc ttgttttccg
     4141 cgcacttgaa gaacggtggc aagacgcgca aattttagct gatggccacc gttgctctcc
     4201 gcagtccatc cgaaaaagtg ggcggcctgt tctatttcgg caggatgctg gacaaaatcc
     4261 gattgcacgc caggggcgaa ttgcccgcgg attatcatga gaacctgggc aaggggttcg
     4321 acgaaaagtg cgtgaccttt ctccgaatca actacgacca attagtcgag cgcgtgaagc
     4381 agggcggctc cgacgaggaa attcttcact ggtgcttcaa ggctggccgg caaccatcgg
     4441 aagatgacat ctacgtctgg aacgaattca tgcgcaaacg aggctggaac gacgaagttt
     4501 ctgagatact aaagcgccgg aaaaaggaag ccggcatgtc cgacaggtcg gacatcgaga
     4561 cggcgtttca gttcatcgac gccgacgaag gccggttgcc gagccgctaa ccgcggatag
     4621 atccgatcca ggggaaagcg ccaattccca aggccggcag ggactgatca ccgatcacta
     4681 atcactgatt acttctgatt ttccctactc cgccaaggta tcgattcgct ccgcgagccc
     4741 gaagtcgagc tcggtcaggc cgcctttgct gtgcgtcgag agaaccaggt gcactttatt
     4801 gtaacggatg tcgatgtcgg gatgatgctc ttcctcttcc gctacctcag ccactgcgtt
     4861 cacgaaatcg atcgcatcgg caaaatcatc aaactcaaat gtgcgctcga tgtgcttttt
     4921 ctccagttcc cactccggga cctttttcat gcgctctttc agctcgcctg ctttgatcaa
     4981 gtcagccata acgggacaac gagtaacatc cgcaaatcgt tttgcaacgc caaaacgagc
     5041 gtctttaaag atcgagaatc ttgcccagat tctttgtcga atcgaagact tcaacggcgc
     5101 agttgcgcgc ctggcgggaa agagtgggcc attcttgaag cgtttgcctg atcgcgatct
     5161 ccgcttcctc aatgcctcgt acaaaatgaa atccgttctc tgatggcagg tactttgccg
     5221 cacctgtatc ttcggtgacg accggccgcc ccagcgcgag aaaggccgcg gcgcgatcgc
     5281 tcagccatcc ggtgcgccat gccacatcta cccccttgat ggctgtgaac tcggcgagcg
     5341 cgcgcgccat gtatcggcgg taggaacccg gagtgcgcgc gacgcggtgc ggatcgacga
     5401 cctgccagcc gcgttgattg aggcgcgccc gctccggatc atccgcctta atattcatgg
     5461 ccagttcgaa acttgcttcc ggaatccgtt ttggaagatc gagataaggt tcgaaggcaa
     5521 atttcttcga cagatctgga aacgcgccgt ccacctctac cgccccgccc caataccatt
     5581 gaccaattgt cgtaaattta gagatgcgtg gacgcggctg gcgatggaga agcttcgtgt
     5641 ccgccaaagg ataaaacgtt ttccagggaa gcgacgattt cggcagccgg caatcgcgcc
     5701 cgtggacgtt gagcccgatc gtccagaact catgatgata cgactggccc atctccattt
     5761 tcgtcatcca ataaaaaatt tcgctcggat ccagatcgca aaagacccgg cgttcgaact
     5821 ggagaagaaa tggcggatga atggagtaac tgaggttgag cagcgcgttt ggaccggcaa
     5881 gccgatcgag cagcgcgcgt ttcgagattc cgatgcaatc cataccgtca agatcatgcg
     5941 tgtcggatgc gggcttttga tgaagcaaac aatatcggcc ggccaatcca tgatcgcgca
     6001 atcttctctg gaaattcgca attttggcct gatcggcacg ggcatcgtta gtcgtcggta
     6061 acaactccag ccaaatcgcg tcgagtttca atcgccgcag tccaagcatc cattgcagcg
     6121 gcaccgagag attgccgccg ccctcgcgat atttcgccgc aaacccgcac ccaagaaaaa
     6181 tcgtcatacg tcgagaatcg cagtccgctg cagtcgctca aacacgggga ctagcacggc
     6241 ttcattgcgt ctttggaagc aatcgcgaga ccagtagcag caacgcaacg acgacaacgg
     6301 cgagaacccc ccaaaaggtg tagcgcgcgg aaccactgag cgtttggata attcgctcgg
     6361 ttttcgaacc tacgttttcc tggggcccga gcgtcgcgga cgttcgttcc gcgcggagaa
     6421 gttgatccgc gatcaggatc acatcataga gcggtgaagc agcgtcggga tttccgtagt
     6481 agagcctgat tggctgcgtc gaatcggacg cagccttgaa cacaacccgg gtgacagggt
     6541 aatgctctcg aaagtcatgc agctcgatcg gtggattatc gccattgtca gtctccagga
     6601 acaacgcgcc actccccggc gcgacatcca gctgaatcgt caattctcgc gttgcctcgc
     6661 cgggcactct tttccaggtc gcgcgaccaa gttcgcgatg aaacgtatcg cctcgctcgt
     6721 ccttcacttc ttcccataaa cgcatctcgc gctggaaaag cgcggaaccg gtagcgcaag
     6781 taagccgcga gatcggaaca cctgcctgtg gcaacttcaa cgaccagcgc gaaagcctcg
     6841 gcttcttcgg atcgctggcc gaggtcggta tcagcggaag cgaacgggaa atggaagtgc
     6901 gctcaaacaa aaatggaagt tgttgatcgt cggttacgat cctcaaatcg cggtggtccg
     6961 gcgccgcgcg ggcgagcaca tcgggatcca gttcgatttg ctgcactcca ggtttcgaca
     7021 gttgaactcg cttgcgaaac ttccacgcgg cgacatcgat gtttgcgccc gctaacggaa
     7081 gcgcagcgag attatccggc ggcttgtaat cgggactcgc gatcaagggc gctggtcgca
     7141 cttcgatggc cgcagcgctt ttcaattggt cgcctaattc cgatagatcg tatcgcggcg
     7201 ccgggcactc attgtttcct gaaagcaatt ggtatcggcc cggctcgcgc gcaaaaaata
     7261 caaggtttgt cacgcgccgc ctggcatcga ccgcattgat agcgagtggc gggctgtcgc
     7321 cgttatcgat aagcacgatt aattcgcgac tgcgaatttg catgtcgatt ggaatttcga
     7381 cttgcgattc agttttgcca tttaggtcca gtcgataaat cgacccagtg cggagcgttt
     7441 gctcgcgaag cccgtcattc cccagttccg gaacagcaat cgtgatcggc cgcgcaaaca
     7501 atgggtccgg cgtctcgatg cgcagagatg cgacggtgag attggtcgcg ccgggatcaa
     7561 ccgacagccg ggttacgccc aaactttcgt cacgcgtctt aattgtgacc ggcaccggct
     7621 cgaccggcgc gtccgtttcg gcgacttgga ggagcacgcc cgtgaacggc accgcagccg
     7681 tgcggctatc gtcgatcgtg aggcgcagaa attcccaaac cccgtcggga aaggaaatac
     7741 tcggattcgc cgcgccgccc gccattgtga aaatcggttt gccggtcgcg agctggtgcc
     7801 aggttgcgcc gtcatgcgaa ccttctacct gcaccgcctt aatgaattca actcccggag
     7861 gcgtttcgaa ggtcacgcct ttgagcaacg attttgttcc cgtgattaac gtaatccgcg
     7921 tggccgttgg ctcgagggca gtgcgcaatt cctgcgaacg cagcgcagat tcgcggcgag
     7981 gcattggctg atcgatcaga taaggcactt cgtttcccgc actacctaaa atgcgcacgt
     8041 ctccgagatc gagacgcgcg gcatcaagcg ttgcggctgg gagattgacg cgaacgagtc
     8101 cgctggacgg cacatcgacg gcctgcctga atcgccattc ggtggacgtc actgcgccaa
     8161 gcgatccggc gcacaaaagc gccgggaccc aaagacctgt ggatacgaat ttcattgggt
     8221 ctgctttgcg gaaaggccga gaaaacgctg atacagaaat gaggcgaaga tcgcgatgat
     8281 tgccaccaca acgaacgcgg cgattcgata aagctgatcc agttgggaaa gatcgtgcag
     8341 gaaaagtttc agaatggtga ctccgagcaa tccaaggctc gcataacgca ccggcctcac
     8401 ggttttacgg atgccaatta ccaggagaag cagcgcgaac aacgcccacg cgatgctgta
     8461 gctcatatca cgcgcgaagt tgccggaaaa ttgaaacgtg agcgtcgcca ttcccgtccg
     8521 gttgaagtaa tcggcaatct caatgttcaa cagcagaaac gcgagaatcg taccaagcgc
     8581 gtaaagcaac ggctgcgcgt tgcgaccgag gacgagattt cgtggagatg ctagcagtcg
     8641 cgcgccagca aacaggcaga cgctcacgat gccgtaggtg taaagatacc aattgaaaat
     8701 cggcgttgcg gaacgggcat gataactgag gaccgccggg tttagtgcga gccgcacaaa
     8761 tgcagttatc agcagtgctg ttccaaccag gcgcagccca ggatgcggca cacggtggaa
     8821 caaccagcag agcgctgcgc cctcaagcgc ccagccgaga gtgatccatt ggcgatcgaa
     8881 ttgaatcgga aaaataaggg tgatgaaaaa cagcgcggcg ccaccaaaca atgcgagatg
     8941 cgcatttcgc gccggacttt ccgccggggt gcgtttcaag agaacgatta gcccgagcaa
     9001 cgacggcaca gcgaacgccg ccggaacgag tcccagcatc ccgttaggat aagcagtgcg
     9061 gatcgcgtca tagacaagaa aaaagtgtaa aggcgcggcg agcgcagccg ctgcccaagg
     9121 cactgtcttt ccggcgaatt gccgctggaa gaggaacggg aagattgtga agacggcagc
     9181 gaacccgatg taccacgcca gggccaagag cacatgatcg ggattgaagt gcgcggaatg
     9241 ccaggcgtat tccagggcga gtgtcgatcc aagaccgacc gctgagagca aatcgagcga
     9301 aaatattttc gccatgccga gaagcagaac cacgaacatg agcgccatac cgaacactgg
     9361 cgctggattc gtcagcggaa gccgcagcgt aaccatgatg agcaagagaa aagggagcgt
     9421 cgcggacaag gtgggaagct ggatagcgaa attggccgga tcggtaagag cgccgaacaa
     9481 tttccctcgt gttttcatta caccactcgg cccgctggcg agcagtggtc ggcacgccca
     9541 gatcgccgcg agtagaaaga aaacggcgaa acatccaaga agaaagagcg aggtgggcaa
     9601 ccccgttagc tcgatcggaa ttcggaaaat ccatccgccg atcaccacaa acgtaagcaa
     9661 caaaacagca aagatcggca acaaggcgcc tgtcgcgacc gctacaaaga tggccagcag
     9721 atccacgcag aacaccatcg gccagaccag gaatgagact tccgacaagc taaagattgg
     9781 cattaaaacc aacaccagcg ccagcgcagg aaatagatga ctccaccact gcgcttcgat
     9841 tctgcgcgtg cgttgcaaaa ccagcgaggc cgttgagtgc agcaacgcga acacgaaaaa
     9901 tgccgcaagc gccacgtaaa gattttgtgt cgtgaggtac cacccggtcc agatcgcgag
     9961 gaacgcgaag acggccagac cggttaccgt gcttaccgcc gcgagttttt ctttcatgaa
    10021 aacaatcgcc agcaggccga tatccgctag gaagagatag ctcagcaaca gcgcaggccg
    10081 atatccaaaa gcctgaaatg acagaaaata aaagccacac gcgatcgccg cgccgcccat
    10141 tccgatggcc gcaactgaga atgcgccttc cgatttcgac gtgcgttttg tcgataccag
    10201 agcggcggaa aagagaaatt cgaagccgag aaaaattgcc atcggcacga gcaccttgtt
    10261 gcccaggaag taatgttctc gcaggaagaa ttgcccgacc caaccgactt gcatcagcac
    10321 ggttccaacc gcgccgagaa cgggcagcgc actccattct ttccggcgcg ccaccatcaa
    10381 gagaccgata tcgagcagtg cgatataacc gaagagaccg agcggattat cctggccagt
    10441 cgaaagcagg attggcgtga ggaaaccgcc ggccataccc agaatcgcca cgacgatcgc
    10501 attcatgcgc accgcaagca ggaacgccac tgccgtgatc agcgtcatca aaagaaacgt
    10561 cggaacaaaa tcgaagaacg cgaaatgata gaacgaccgg cacgcgaacg tgacggcata
    10621 tagaatcagg attccggtcg cgcaaagtgt ttgcgccgtg accacgttct cctttcgcct
    10681 cagccccagg ccaccaacga tcaaagccag acccactgca aacccgatcg ccacgcgcac
    10741 ttccggtgga atgagattgt gctcgaatga atatttcacg aaaaaggcca cgccgaggaa
    10801 cagcgccagt ccgccgatcc atgcgaagag cttcgcgccc atgaattgct cccagttgat
    10861 cgcgggtaac aaaggttggg tcggtgccga ctcgccggaa ggcggctgag gaggaatcgg
    10921 cggtggcgta gaggtcgcta cagccgcgac ctcctctggt aatggcggcg gaactgaaac
    10981 gggtcgtgcc tccgagggcg ttgcaatcgg cgcgacgaaa ggctcgcgtt ccgcggtttc
    11041 cctttccgcg gcaaatggcc gctctgccga tggttcgccg ggcgcgcgtt tcaacgtctg
    11101 cagttccgct tccagattac gtaacctggt tttgaagtct tcaacgcttc gccgcgcact
    11161 cgccgccttt gcgattgcga ccagaggcaa aacgaaacat gcggcgatta caatcaggag
    11221 cagaagggcg aaagcttcca taaattaaag gtgtgcgaac cggtttacat aatgcacggt
    11281 ttggcgcagt tgcgtgaatc gataaatttg ccggtcaaca tcgaattatt cgatcatgaa
    11341 cattcatcac ctcgagctct tctattatgt ggcaaaacat ggcggcattg cggccgctgt
    11401 gcgcaagatg ccgtacggaa ttcagcaacc agccgtgagc ggacaaatcg ctagactcga
    11461 agaagccctc ggcgcgaaat tattcaatcg aaggccgttt tctcttcttc cggcgggtgc
    11521 agagctattc gaattcatcc gaccgttttt cgatgacgtg gagaaagtcg ccggcagaat
    11581 ccggggcgct tccctgcaat tgcgcatcgc tgcgccttcg gtcgtgctgc atgattacgt
    11641 gcccgaatta ttgcggcgga ttcgccggcg gtttccctct ttccggcttc aacttcacga
    11701 ggcagtgcga gccgacgccg agaggctgtt gctggcggga gagatcgatc tcgcgatcac
    11761 gatccttgat ggcaagcggc gtccggagat tcattcccgt cccctgctcg aactcccaat
    11821 gatattgctg gtaaacaaaa agcagaacat tacgcgcgcg gagcagctat gggcccggga
    11881 caaaatcgaa gagacgctaa tcacgttccc gcgtggtgac gcggtgcagg cacactttca
    11941 gcgaggcctc cagcagcttg gagtggaatg gtttcccgga attgaagtta atacaacccg
    12001 gttaatcgaa ccctacgtca ctcacggcta tgggatcgga ttgaccgttg ccccacctag
    12061 tttcaaatct ccacccggag tgcgcgcgat cccattgcca gggttttcgc cggtgatcgt
    12121 cggagtagcc tgggccggaa agctgtcgcc gatcgcccaa caatttctcg ccgaggtcga
    12181 aatcgaagcc gacgctgtaa aacgaagctc aaaatgccgc tagcgctcca gcaactagct
    12241 caggcacgag caattctagt tttagaagat acggccgtaa gggatgtgcg ctttgccggt
    12301 gaacacctga ggcggccttg cctcaccggt gcaaagaccg gcattcggca ccggaatcac
    12361 tgatcccagg aattgcggct gcctacgcac attcaattcc tgctcgagat caaagccgaa
    12421 accaatcaat tctggttcgt gcaggaaggt gctgtacatc agcatgccag ccggcctgcc
    12481 attgtcgcga atgccgaccg gaagcgataa gttcggataa ccagacaccg ctggccctgt
    12541 cgaattcgtc agatgcggtg ccacaattgc gtcgagatcg tcggcagcga gtgcatcatc
    12601 aattccggaa cgcgccgtat tccgagcatg cgttcgggcg gctatgtaat ttggatcgtt
    12661 gggatagcca gggaactgtt ccgacaattc gaagacctcc tgaccgtaga aaaccagttc
    12721 ctgctcgcaa tgcgcgttgt tgaatgcaat cagatccgcg agagttcgca ggtttgtgtg
    12781 cgtgagcgtt gcgaggtact ccgcgatttg cgccctaaat tcatagagca acgcggtgaa
    12841 ctcgtcatca gtgtaggcga atacgtcacc ggtatcggta tctacg
//
LOCUS       DASQUQ010000191         5483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_699857, whole genome shotgun sequence.
ACCESSION   DASQUQ010000191 DASQUQ010000000
VERSION     DASQUQ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699857"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..281
                     /gene="rpsS"
                     /locus_tag="VE486_05465"
     CDS             3..281
                     /gene="rpsS"
                     /locus_tag="VE486_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZA38568.1"
                     /translation="MARSLKKGPFVESHLLEKIDKMNSSGAKRPIKTWSRRSMVTPDF
                     VGHTFLVHNGRTFVSVFITENMVGHKIGEFSPTRVFKKHGSHTAKVTK"
     gene            331..1122
                     /locus_tag="VE486_05470"
     CDS             331..1122
                     /locus_tag="VE486_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38569.1"
                     /translation="MSRGSIIMLVGLVAVGRLAAEGNSSLSESKIENANRAGTGETLL
                     TQNEQLRRQLSIDQESLKVLTSSLAQSNEAAELFRRKYSDLELRMEALGLAAANKDRA
                     KLEQRLLAAVSDLQLAQKERDDYRDQMLRLNEAMLRFLKTSQSGDAQARMDVETQLRS
                     TNELVARSHDAPDSPEPGLMDGSVISMKEEWSFVVGNLGEKQGVKIGMPMRVMRDDRK
                     IATLRVVDVRQRICGAVIQELDSEKEKIKVGDRLQVDAQSNVSLK"
     gene            1129..1455
                     /gene="rplV"
                     /locus_tag="VE486_05475"
                     /pseudo
     CDS             1129..1455
                     /gene="rplV"
                     /locus_tag="VE486_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            1645..2337
                     /gene="rpsC"
                     /locus_tag="VE486_05480"
     CDS             1645..2337
                     /gene="rpsC"
                     /locus_tag="VE486_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZA38570.1"
                     /translation="MGQKVNPIGFRLGVNRDWRSRWYASPQELPAFLHSDLEIRNYVK
                     KKLQFAAVSKIVLERAWNSIRVTIFTARPGIVIGRKGAEIEKMTEEISKMAQGKQVKI
                     DIQEIKSPELDAQLVAENVALQIERRIAYRRAMKKAVQTAMDFGAEGIRLRCSGRLNG
                     AEISRSEWYREGKVPLHTLRTAVDYGFAEANTVYGKIGVKCWICKKEEAPAQSATPPP
                     PSVLPPPVPEAV"
     gene            2350..2775
                     /gene="rplP"
                     /locus_tag="VE486_05485"
     CDS             2350..2775
                     /gene="rplP"
                     /locus_tag="VE486_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZA38571.1"
                     /translation="MPLMPKRVKYRKSQRGSRKGTASRNLRIDFGEFGLQTLERAWIT
                     NTQLEAARVALTRNMKRKGKLWIRIFPDKSVTGRPPETRMGKGKGQPEFWVATVRPGN
                     ILFELDGVPESVARESLRLAADKLPVRTKFLSRHHVAAA"
     gene            2953..3150
                     /gene="rpmC"
                     /locus_tag="VE486_05490"
     CDS             2953..3150
                     /gene="rpmC"
                     /locus_tag="VE486_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZA38572.1"
                     /translation="MKEITELSTDELLTKRRDLRQESLHLRLQQQSGQLEQPSRLRLL
                     RRDVARIETVLSQRQKETEKA"
     gene            3156..3446
                     /gene="rpsQ"
                     /locus_tag="VE486_05495"
     CDS             3156..3446
                     /gene="rpsQ"
                     /locus_tag="VE486_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZA38573.1"
                     /translation="MAEQHQQPPPLPVAVTRGSRKTRTGEVISSSMNKTIIVRTVTRV
                     PHPKFGKIVKQMKKFYAHDEQNEAKTGDMVRIMETRPLSKLKRWRLVEVVQK"
     gene            3481..3846
                     /gene="rplN"
                     /locus_tag="VE486_05500"
     CDS             3481..3846
                     /gene="rplN"
                     /locus_tag="VE486_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZA38574.1"
                     /translation="MVQLRSRLDVADNSGARMATMIGVIGKSTLYAGIGDVITANVKE
                     ASANGTVKKGEVVRAVLVRTKQPIRREDGSLLRFDNNAIVIIDKDLNPRGTRIFGPVA
                     RELRERNFMKIVSLAPEVL"
     gene            3843..4136
                     /gene="rplX"
                     /locus_tag="VE486_05505"
     CDS             3843..4136
                     /gene="rplX"
                     /locus_tag="VE486_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZA38575.1"
                     /translation="MNRIKCHVKKGDHVEVVSGNFRGSSGKILAVFPHKQRVLVEGVR
                     IIKKHLRKSQDNPSGKIAEREGPIHISNVKLIERDGKPVKGTEPKAKREKKKS"
     gene            4153..4722
                     /gene="rplE"
                     /locus_tag="VE486_05510"
     CDS             4153..4722
                     /gene="rplE"
                     /locus_tag="VE486_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZA38576.1"
                     /translation="MENEFYRDYKERVMPAMQKLHGYKNVHQIPKVEKVVINTSVGSQ
                     SDVKQALEEAKAELALITGQRPVETLAKKSISNFKLRKDQAIGAKVTLRGERMYEFLE
                     RFIKAALPRIRDFRGVSPRCFDEHGNYTLGVSDQSIFPEVELDKIKRNIGFDVTIVTT
                     ARTDEEAKSLLSEMGMPFSDRGRPAAQAA"
     gene            4726..5124
                     /gene="rpsH"
                     /locus_tag="VE486_05515"
     CDS             4726..5124
                     /gene="rpsH"
                     /locus_tag="VE486_05515"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZA38577.1"
                     /translation="MSTVTDPIADLLTRVRNGTRAQKPEVLIPYSKIKAEIARILKEE
                     GYISDYAIDTSGPHPRIKVTNKIVNRSSAIADLRRISRPGLRRYVGADEIPRVLGGMG
                     LAILSTSRGILSGREARKQKIGGELLAYVW"
     gene            5130..>5483
                     /gene="rplF"
                     /locus_tag="VE486_05520"
     CDS             5130..>5483
                     /gene="rplF"
                     /locus_tag="VE486_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZA38578.1"
                     /translation="MSRIGNKEIEIPDKVSVSIGNDGAVSVEGPRGKLNWKLPRDIKA
                     TVKDNRVSLVREAETRSAKALHGLSRSLVHNMVQGVSQGFTRQLEIEGVGFKAAVQGP
                     ILNLSLGFSHPIQFQI"
BASE COUNT         1439 a         1343 c         1622 g         1079 t
ORIGIN      
        1 ttatggctag atcgttgaag aaaggaccct tcgtcgagtc gcatcttctg gagaagatcg
       61 acaagatgaa tagttcgggc gcgaaaagac cgattaaaac atggtcgcgg cgttcgatgg
      121 tgacgccgga ttttgtcggc cacacgtttt tggtgcacaa cggaaggacc tttgtctccg
      181 tcttcatcac cgagaacatg gtcggtcata agatcggtga gttttcgccg acgcgggtgt
      241 tcaagaaaca tggctcccac acggcgaaag tgaccaagta atgtgaattt tcgattttcg
      301 attttcgatt gtcgattgcc cagatgggca atgagccgtg gatcgataat catgcttgtg
      361 ggtctggtgg ccgtcggaag gctggcggca gagggaaata gttctttgtc agaatccaaa
      421 attgaaaatg cgaaccgcgc agggacgggt gagacattgc tcacgcaaaa cgaacaattg
      481 cgaaggcaac tgtcgatcga ccaggaatcg ctgaaggtgc tgacgagcag cctggcgcaa
      541 tccaatgagg cggcggaatt gtttcgccgc aagtactccg atttggagtt gcggatggaa
      601 gcgctcggtc tggcggcggc gaacaaggat cgggcgaagc tggaacagcg tttgctggcg
      661 gcagtgagcg atcttcagct tgcacaaaag gaacgggatg attatcgcga ccagatgttg
      721 cgattgaacg aggccatgtt gcggtttttg aaaacgtcgc agagtggcga cgcgcaagcg
      781 cggatggacg tggaaacgca actgcgcagc acgaacgagc ttgtcgcaag atcgcatgac
      841 gcgccggatt ctccggagcc cggtttgatg gatgggagcg tcatcagcat gaaggaagaa
      901 tggtcgttcg tggtcggcaa tcttggcgaa aagcaaggcg tgaaaattgg aatgccgatg
      961 cgtgtaatgc gcgatgaccg aaagatcgca accctacgag tagtagatgt gaggcagaga
     1021 atctgcggcg ccgtgattca agaactggat tcggagaagg aaaagataaa agtaggcgac
     1081 cggctccagg tggatgccca gtcgaatgtg agcttaaaat aaatatagat ggaagtgaga
     1141 tcgatatata agtatgcgcg gatttcgcca ttcaaggtgc gcgaagtcac gcgtgagatc
     1201 cagggtctgc cggttgcggc cgcactcgac cttctggctt tcactcctaa aaaggcatcc
     1261 tttttgatcg gcaaaacgct taagagcgca atcgctaacg cggagaacaa tgccaatctg
     1321 aaaccggacg gcctggtgat aaaggaagcg attgtcggcg agggcccaac gctaaagcgg
     1381 atgatggcgc gcgcacgtgg cagtggcagc ggcatcctta agcggaccag ccacattcga
     1441 atcgtgctct ccgatgaaat tccaattgag acgcgcgaaa ccagaaaagc caaacgcgaa
     1501 caggaaaaga aagcggcgag taagcaaggg gagggcaagg cgacggaagc atcaacaacc
     1561 acctctgaga aaaaaacggc gaaaccgaag aaggccacga aggcaaagtc gaagaaatga
     1621 atttaactat ggattgtcat tgttatggga caaaaagtta atccaatcgg atttcgcctc
     1681 ggtgtgaacc gcgactggcg ctcgcgctgg tacgcctcgc cgcaggaact gcccgcgttc
     1741 ttgcacagcg atttggagat tcgcaattat gtgaagaaaa agctgcagtt cgctgctgtc
     1801 tccaaaattg tgctcgagcg cgcgtggaac agtattcggg tgacaatttt taccgcgcgt
     1861 ccagggatcg taatcgggcg caaaggagcg gagatcgaaa agatgactga agagatctcg
     1921 aaaatggcgc aggggaagca agtcaaaatc gatatccagg aaatcaaatc gccggagctc
     1981 gatgcgcaac tggtcgccga gaacgttgcg ctgcaaattg agcggcgtat tgcttatcgc
     2041 cgtgcaatga aaaaagcggt gcaaaccgcg atggattttg gcgcagaggg cattcgcctg
     2101 cgttgttcgg gacgtctcaa tggtgcggag atctcgcggt cggaatggta tcgggaaggt
     2161 aaagttcccc tgcatacgct gcgcacggca gtcgactacg gcttcgccga agccaacacg
     2221 gtttacggca agatcggggt gaaatgctgg atctgtaaaa aggaagaagc gccggctcaa
     2281 tcggccacgc caccaccgcc atcagtcctg ccgccgccag tccctgaggc agtttaaagg
     2341 agaattgcta tgccattaat gccaaaacgc gtgaagtatc ggaagtccca gcgaggaagt
     2401 cgcaagggaa ctgcttctcg taatctaaga atcgattttg gcgagtttgg tttacagact
     2461 ctcgaacgcg cctggatcac taacacgcaa ctggaagctg cacgggtggc tctaacgcgg
     2521 aatatgaaac gcaaaggcaa actctggatt cgcatctttc ccgataaatc ggtcacgggg
     2581 cgtccgccgg aaacccgcat gggcaagggc aaaggccagc ccgagttctg ggtcgcgaca
     2641 gtgcgccccg gcaacatctt gttcgaactg gatggcgtac ccgaatcggt cgcgcgtgaa
     2701 tctcttcggc tggcagcaga caagttgccc gtgcgaacaa agtttctgag ccgacaccac
     2761 gtcgccgctg cctaaccagc catgaaactt ttttgtcatt ctgagcgaag tggagcgaag
     2821 cgaaacgcag tcgaagaatc tctcactacc atctggtcgc cgctgtgcca gagaagagtt
     2881 agagatgtct cgatttcgct cgacatgaca atggattggc atctcgtcga cgctgcgtaa
     2941 tgagccatga aaatgaaaga gatcactgaa ttgagcacgg acgagctgct gaccaagcga
     3001 cgcgacttgc ggcaggaaag cttgcatctg cgcttgcaac agcagagcgg tcagctcgag
     3061 caaccaagcc ggttgcgttt gctgcgacgc gacgttgccc gcatcgagac cgtgctctca
     3121 cagcgtcaga aagagactga gaaagcgtaa acattatggc cgagcaacat cagcaaccgc
     3181 ccccgctgcc cgtagccgtc acccgtggct cgcgcaagac gcgcaccggc gaagtgattt
     3241 ccagcagcat gaacaagacg attatagtac ggacggtgac gcgcgtgccc caccccaagt
     3301 tcggcaaaat cgttaaacag atgaaaaagt tttacgcgca cgacgagcag aatgaggcga
     3361 aaactggcga catggttcgg atcatggaga cgcggccact gagcaagctg aagcgctggc
     3421 gtttggtaga ggtcgtccaa aagtaacttc gaaaggattt tgcagtaaat tatcgatctt
     3481 atggtgcaac ttcgatccag actggatgtg gcggacaaca gtggggcgcg catggcgacg
     3541 atgatcggcg tgatcggcaa gtccacgctt tatgccggaa tcggtgatgt gattacggcg
     3601 aacgtaaaag aagccagcgc gaacggaacg gtaaaaaaag gcgaagtcgt gcgtgcagta
     3661 ctggttcgca cgaaacagcc gattcgtcgc gaagatggct ctctgctccg ttttgataac
     3721 aatgcgatcg tgattatcga caaagatttg aatccgcgcg gcacccgcat ttttgggccc
     3781 gtggcccgcg aattgcgcga gcggaatttc atgaagattg tctcgctcgc tccggaagtg
     3841 ttatgaaccg tatcaaatgc cacgttaaaa aaggcgacca tgtcgaggtc gtttcgggaa
     3901 attttcgcgg ttcttcaggc aagattctgg cggtgttccc gcataagcag cgtgtgttgg
     3961 ttgagggcgt gcggattatt aagaaacatc tccggaaatc gcaggacaat cccagcggca
     4021 agatcgcgga acgcgaaggc ccgattcata tttccaatgt gaaattgatc gaacgcgacg
     4081 gaaagccggt gaaaggcacc gaaccgaaag ccaaacgaga gaaaaagaag agctaggaca
     4141 gcttgttgag aaatggaaaa cgaattttat cgcgattaca aagaacgggt catgccggcg
     4201 atgcaaaagc tgcacggcta taagaacgtc caccagattc caaaggtcga gaaagtagta
     4261 attaacacct cggtcggctc gcaatcggac gtgaagcagg ctctggagga ggccaaggcg
     4321 gagcttgcct tgatcaccgg ccaacgcccg gtggaaacgc tggccaagaa aagcatttcc
     4381 aatttcaaat tgcggaaaga ccaggcgatt ggcgcgaagg tgaccttgcg cggtgagcgc
     4441 atgtacgaat ttctcgagcg cttcatcaaa gcagccctgc ctcgcattcg tgattttcgc
     4501 ggcgtgtcgc cgcgctgttt cgatgagcat ggcaattata ccctgggagt ttcagaccaa
     4561 tcgatctttc ccgaggtcga gctcgataaa atcaagcgca acatcggatt tgatgttact
     4621 atcgtcacca cggcacggac cgacgaggaa gccaaatcgc tcttgagcga aatgggaatg
     4681 ccgttcagcg accggggaag gcccgccgcg caagccgcct gattcatgag tactgttact
     4741 gatcccattg ccgatcttct gacgcgtgtc cgcaacggca cccgtgcgca aaagcccgag
     4801 gtgttgattc cgtactcgaa gatcaaggca gagatcgccc gcatcctgaa ggaggaaggc
     4861 tatatctccg attatgcgat cgacacgagc ggcccgcatc cgcgcataaa ggtcaccaat
     4921 aaaatcgtca atcgctccag cgcgattgca gatctgcgac ggataagccg gcctggactg
     4981 cgccgttacg tcggcgcgga tgaaatcccg cgagttctgg gtgggatggg cctggcgatt
     5041 ctttccacat cgcgcgggat tttatcaggg cgcgaagcgc gaaaacaaaa aatcggcggc
     5101 gaactgctgg cctacgtttg gtaaatttta tgtcacgaat cggaaataaa gagatcgaga
     5161 ttcccgacaa agtgagcgtc agcatcggca atgacggggc ggtatcggtg gaaggaccca
     5221 ggggaaaact gaactggaag ctgccgcgcg acatcaaggc aactgtgaaa gacaaccgag
     5281 tctcgctcgt gcgcgaagcc gaaacgcgca gcgcaaaagc gctgcacgga ctctcgcgca
     5341 gcctggttca caacatggtg cagggggtca gccagggctt tacccggcag ctcgagatcg
     5401 aaggggtcgg tttcaaggcc gccgttcaag ggccgatact taatttgagc ctcggtttct
     5461 ctcacccgat tcagtttcag att
//
LOCUS       DASQUQ010000192         9767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_861033, whole genome shotgun sequence.
ACCESSION   DASQUQ010000192 DASQUQ010000000
VERSION     DASQUQ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861033"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1912)
                     /gene="aspS"
                     /locus_tag="VE486_05525"
     CDS             complement(140..1912)
                     /gene="aspS"
                     /locus_tag="VE486_05525"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZA38579.1"
                     /translation="MYRTHDCNSLRKTDIGREVALAGWVNVLRDHGGVIFIDLRDREG
                     LTQVVFRPEENAALAKQAHSLRSEDVIQVSGRVAARVPGTENPNLTTGDIEVIPDHLR
                     ILNRADTLPFPIDTEPHNEDLRMTYRYYDLRRPRMARNLRLRHIATKATRDYLDSEGY
                     VEIETPILSKSTPEGARDFLVPSRLTPGKFYALPQAPQQYKQLLMVAGVEKYFQIAKC
                     FRDEDLRADRQPEFTQIDIEASFVTPDDIFAVTEGMLAAIFKAALNVNIKTPFDRLTY
                     RDAVSRYGSDKPDRRFGMELVDLGEDFRVSSFKVFRGTLDAGGVVKAINAKGFAGITI
                     GQADELTEIAKLHGAKGLAFIKIEGGEWKSPVVKFFSEAEKAALQSKLNIEEGDCVFF
                     AADKWEIACEVLGRLRLRIGEMKNLTAASEIWDFLWVTDFPLFEWSPEENKWNAMHHP
                     FTRPKAEDMPLFEAKKFPEVRAEAYDIVLNGVEIGGGSMRIYEPELQEKMFDALGINP
                     EAQQSQFGHLLRALRLGAPPHGGIAPGLDRIVMLICGEQNIREVMAFPKNNRGMDLMA
                     NSPAEVDPRQLRDLSLKLASEKKV"
     gene            complement(1991..3241)
                     /gene="hisS"
                     /locus_tag="VE486_05530"
     CDS             complement(1991..3241)
                     /gene="hisS"
                     /locus_tag="VE486_05530"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZA38580.1"
                     /translation="MQALPGFRDFLPDQCAARNYVFAGWREVARRYGFVEWEGPVLES
                     TELYQKKSGAEIVDQLFHFTDKGEREVALRPELTPTLARVVAAHEREFKKPFKWFSIG
                     QFFRYEKQQRGRLREHFQLNCDIIGEADLAADVELVALCIDILCAFGFTEKDFVVRIS
                     DRDFWTKFLREENVSPDRWDELLQAIDKSGREPREKTAEKLGKLADPVFALLKSGGKS
                     EKLEKLVDSLGIRGLGEFVAMDVGIVRGLAYYTGVVFEVFDRAGKFRAIAGGGRYDNL
                     IGQLSDGAVSMPALGFAMGDVVLGELINETPHAREKMQQAIATERKIDIYVVIAKEER
                     RPDALAQIQQLRDRGYRVDYPLTPTKVARQFKAAEDAGAALALLFGDEWPQVKVKDLA
                     TRKETLIAREELLAHLQAQSSLSS"
     gene            3401..3823
                     /locus_tag="VE486_05535"
     CDS             3401..3823
                     /locus_tag="VE486_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38581.1"
                     /translation="MKQTGLTKILVAAVACAAAFGMALAQETTTTTTTDPVTGTTQQT
                     TVTSAGTITAYTPDSDYIMFRTSADAPPVRYYYTKDTTILDPEGHTVTWSAVRPDIPA
                     TVYYTNVGDRVVVRKIVLAKPTTVYKKEETTTTTTREP"
     gene            complement(3974..4945)
                     /gene="galE"
                     /locus_tag="VE486_05540"
     CDS             complement(3974..4945)
                     /gene="galE"
                     /locus_tag="VE486_05540"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZA38582.1"
                     /translation="MKILVVGGAGYIGSICAELLLDQGHEIAVFDNLCEGHRSALDPR
                     ARFIEGDLQDRQAIDSALALAQPEAVMHFAASALVSESMRDPSKYFRNNISNGLNLLD
                     AMVAARVQRIVFSSTCAIFGPPERLPIDETAPRRPINPYGESKLAFEKILRWYDEIHG
                     LKFVSLRYFNAAGASANFGEDHRVETHLIPNTLKVALGQKPHVEIFGTDYETPDGTCI
                     RDYIHIVDLARAHILALGAPRSELYNLGTGGGASVRDVIAACRKVTGKKIDTVERPRR
                     PGDPPRLIASSDKIKNELGWQPQFQSLDAIIESAWKWHQKFPNGYGD"
     gene            complement(5048..5566)
                     /locus_tag="VE486_05545"
     CDS             complement(5048..5566)
                     /locus_tag="VE486_05545"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HZA38583.1"
                     /translation="MSKCLNLTLAASAACLIVGCATQPRTDLTSAQRTQRVRTTAYTH
                     TEKGGPRNALGDRLSSHHVMSAASDWSRFPLGTHFRIADTNEEYIIDDYGTALIGTDT
                     IDLYKTNRLAMKKWGVRHVDIAILKWGSEEESLKVLKPRCKHRCARQMVVSLEKKRGK
                     TVAQASSARTSL"
     gene            complement(5685..6152)
                     /gene="mscL"
                     /locus_tag="VE486_05550"
     CDS             complement(5685..6152)
                     /gene="mscL"
                     /locus_tag="VE486_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZA38584.1"
                     /translation="MKKIWNEFKEFAVKGNAVDLAVGVIIGAAFGSIVNSLVKDVVMP
                     PISLLTGGLDFSNQFIILRAAKDGSTAFNTPADAVKAGAITWNYGNFMTLLINFLIVA
                     GAIFLLVRGINKMRGPAQKEPASKECPACAMTIPIRATRCPHCTTELAQTATR"
     gene            complement(6149..8830)
                     /locus_tag="VE486_05555"
     CDS             complement(6149..8830)
                     /locus_tag="VE486_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZA38585.1"
                     /translation="MNERRKPYPFDQIEPKWQKIWDERQLFHASNPGEKHFDPKRPKF
                     YILDMFPYPSGAGLHVGHPEGYTATDIVARYKRMRGFNVLHPMGWDAFGLPAEQYAIK
                     SGQHPAITTRENVAKFKSQLKRIGFSYDWQREINTTDPRYYKWTQWIFLQIYNSWFNP
                     DTNKAEPISTYRGTDPDSVRLAYVADFPVNWCPELGTVLANEEVVDGKSEVGGFPVVR
                     RPMRQWMLRITAYAERLIDELDGLDWPESIKLLQRNWIGKSEGAEIDFAVAGVGDPGA
                     AATNSGYKIRVFTTRPDTLYGATYMVVAPEHSLIDLVVSEEQWPAVREYRERAARKSD
                     LERTELAKEKTGVFTGAYAINPVNNERIPIWVADYVLPGYGTGAIMGVPAHDERDLEF
                     ARKFNLQIREVVQPPGDQDPIGFVGEGIATNSPVINGLPSREAIRKITEWLEQRGLGK
                     GAINYKLRDWLFSRQRYWGEPFPIVWEKGKHRAVDEKELPVMPPPLEDYKPTGTGEPP
                     LARAKEWVRYSDKAVRETNTMPQWAGSCWYYLRFCDPQNDERFVGKEAERYWMGGENG
                     AAPMTHEEGMTKSETRSAGDEAGHSNIDSSLSKRPGGVNLYVGGTEHAVLHLLYARFW
                     HKVLFDLGYLSKPEPFQRLVNQGIILGEDNQKMSKSRGNIVNPDTVIDQYGADAFRCY
                     EMFMGPLEQMKPWSMRGVEGVARFLARVWRLFMTENQAGDWELSARVKDVEPDKAQQK
                     VTHATIKKVTDDIESFSFNTAISQMMIFVNEFTNTETLPLPAMRRFLVLLNPFAPHLT
                     SELWEKLNATFRDDAGEISGQQWPDYDERFLVEDEVEMVIQVNGKVRDRMKMPILATE
                     EETKTAALAHSKIQKLTAGKTIRKIVVVPGKLVNIVV"
     gene            9099..9710
                     /gene="rdgB"
                     /locus_tag="VE486_05560"
     CDS             9099..9710
                     /gene="rdgB"
                     /locus_tag="VE486_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZA38586.1"
                     /translation="MQHLIVATRNAHKTREIQRILGADFSVSDLSAYPEISETLESGK
                     TFEENAILKAVTVSGQVKGLVVADDSGLEVDALGGAPGIHSARYAGANASDKEKIDKL
                     LSELGKISAKGDQPVARFRCVLALARDGQILGTFEGVVEGEIAGQARGSHGFGYDPIF
                     LPRGFGRTFGEFAPAEKDWWSHRARALEKLRAFLNTVPGSAKK"
BASE COUNT         2129 a         2701 c         2644 g         2293 t
ORIGIN      
        1 gcgtaaacga acgcaaagac atcgagaaat aacttaaagc gcgacgaggg ctgattagaa
       61 ttcttgcccg gctcaagctc ttctgtagct gacactccga actccgaaaa ttttcgcaag
      121 cgcgagcaag cagttgccgc tacacctttt tttcgctcgc cagtttgagc gacagatctc
      181 gcaattggcg tggatcgact tcggcgggcg agttggccat caaatccatg ccacggttat
      241 ttttcgggaa cgccatcacc tcgcggatgt tttgttcgcc gcaaatgagc atcactatac
      301 gatcgagccc aggcgcgatg ccgccgtgcg gcggcgcacc gagccgcagc gcgcggagca
      361 aatgaccaaa ctgcgattgc tgagcttccg ggttgatccc gagcgcgtcg aacattttct
      421 cctgcaactc aggctcgtag attcgcatgc tgccgccgcc gatttcgacg ccgttcagaa
      481 cgatgtcgta ggcctcggcg cgaacttccg gaaatttctt tgcctcaaat agcggcatgt
      541 cttcagcttt cgggcgggtg aagggatgat gcatggcgtt ccatttgttt tcctcgggac
      601 tccattcaaa cagcggaaaa tctgtgaccc aaagaaaatc ccagatttca gatgcggcag
      661 tcaggttttt catttcgccg atgcggagcc gcaatcggcc aagaacttca caggcgattt
      721 cccatttgtc ggcagcgaag aaaacgcagt cgccttcctc gatattcagc ttcgattgca
      781 gcgcggcctt ctcagcttcg ctaaagaatt ttacgacagg cgatttccat tcgccgccct
      841 cgatcttgat gaaggcgagt ccctttgcgc cgtgcaactt cgcaatttcg gttagttcgt
      901 cggcttgtcc gatggtgatt ccggcaaacc ctttcgcgtt gatcgctttt accacgccgc
      961 cggcatcgag cgttccgcgg aaaactttga agctgctcac gcgaaagtcc tcaccaagat
     1021 cgacaagttc cattccgaac cggcgatccg gtttatcgct accgtaacgg ctcactgcgt
     1081 cgcgataagt gaggcgatca aaaggcgttt tgatgtttac gttaagcgcc gccttgaaaa
     1141 tcgccgccag cattccttca gtgaccgcga aaatgtcgtc cggtgtcaca aaagaagctt
     1201 cgatgtcgat ctgggtaaat tcgggttgtc ggtcggcgcg caaatcctca tcgcgaaaac
     1261 atttcgcgat ctgaaaatat ttttccacgc cggccaccat gagcagttgt ttgtattgct
     1321 gaggagcctg gggcagcgcg taaaattttc ccggcgtcaa tcggctcggc accaaaaaat
     1381 ctcgtgcacc ttcgggcgtg ctcttggaca gaatcggcgt ctcaatttcg acgtagcctt
     1441 cgctgtcgag ataatcgcgg gtcgctttcg tcgcgatgtg gcggaggcgc aggttgcgcg
     1501 ccatccgcgg tcgccgcaag tcgtaatagc ggtaggtcat ccgcaaatct tcgttgtgcg
     1561 gttcggtgtc gatcgggaac ggcagcgtgt cggcccgatt taaaatgcgc agatgatccg
     1621 gtatgacttc gatgtcgccc gtcgtgagat ttggattttc cgtgcctggc acacgcgcag
     1681 ccactcgacc cgaaacctgg atcacatctt cactgcgcaa actatgcgct tgtttcgcaa
     1741 gagccgcgtt ttcctcagga cggaaaacga cctgggtcaa accttcgcga tctcgcagat
     1801 cgataaagat gacgccgccg tgatcgcgca aaacatttac ccaaccggcc aacgcgactt
     1861 cgcggccaat gtcggttttg cgcagggaat tacagtcgtg cgtgcgatac atcttttttt
     1921 gtcattttga gcgaagtcga aacatctctg aaaataaaga gtcagatcct tcgacttcgc
     1981 tgcgctgcgc tcaggatgac aagctggatt gcgcttgcaa gtgcgcaagc agctcttctc
     2041 gcgcaatcag cgtttctttg cgcgtggcga ggtcttttac tttcacctgc ggccattcgt
     2101 cgccaaagag aagcgcaaga gctgcaccgg catcttcggc ggctttgaat tgcctggcga
     2161 ctttggtcgg cgtgagcgga taatcgacgc ggtagccgcg atcgcgcagc tgttgtattt
     2221 gcgcgagtgc atcggggcga cgttcctctt tggcgatgac gacgtagatg tcgatcttgc
     2281 gctcggtcgc gattgcttgc tgcattttct cgcgcgcgtg cggcgtttcg ttgatcagct
     2341 cgccgagcac gacgtcgccc atggcgaacc cgagtgcggg cattgaaacg gctccatcac
     2401 tgagttggcc aatcagattg tcatagcgac cgccgcctgc gatggctcgg aattttccgg
     2461 cgcggtcgaa cacctcaaaa acgacgccgg tgtaataagc aaggccgcga acgataccga
     2521 catccatcgc gacgaattcg cccagccccc gaatgcccag actatccaca agtttttcga
     2581 gcttctcgct ttttccgccg ctcttcaaaa gcgcgaagac cggatccgcg agcttgccaa
     2641 gtttctcggc tgttttctcg cgcggctcgc gcccggactt gtcgatcgct tgcagcaact
     2701 cgtcccagcg atcgggtgag acattttcct cgcgcaggaa ctttgtccag aagtcacgat
     2761 cgctaatccg gacgacgaaa tctttttctg tgaaaccgaa tgcgcataag atatcgatgc
     2821 aaagtgcgac cagttcgaca tcagcggcaa ggtccgcttc gccgatgatg tcgcaattga
     2881 gttggaaatg ttcgcggaga cggccgcgct gttgtttttc gtagcggaaa aactgaccga
     2941 tggaaaacca tttgaacggc ttcttgaatt cgcgctcgtg tgcagcgacc acgcgcgcaa
     3001 gcgttggcgt cagctcggga cgcaacgcga cttcgcgctc tcctttatcc gtgaaatgga
     3061 aaagttgatc gacaatttct gcgccgctct ttttttggta taactcggta gattcgagaa
     3121 cgggaccctc ccattccaca aaaccgtagc ggcgcgcgac ttcgcgccag cctgcaaaga
     3181 cataatttcg cgccgcgcat tgatccggaa ggaaatcacg aaaaccaggt aaagcctgca
     3241 tacagatcac gttacattgg cgcaggaaag cgattacgcc cctgaatgcg accacgcgag
     3301 ccggaaatta cgatatcagg gcgtagctga aattccggcg ctaacgagcc gggcaggtac
     3361 aggatggaga acatcattta atcaaagcag agggacaaat atgaaacaaa caggattaac
     3421 aaaaatattg gtcgcggcag tggcctgtgc ggcagcgttt gggatggcgt tggcacagga
     3481 gacgacaacc acgacgacaa cggatccggt gaccggcaca acgcagcaga ctaccgttac
     3541 gagcgcaggc acgattacgg cctacacgcc ggactcggat tatatcatgt tccgcacgtc
     3601 agcggacgcg cccccggtgc gctattacta cacaaaagac acgaccattc tggacccaga
     3661 agggcatacc gtaacctggt cggccgttcg tcctgacatc ccggccaccg tctattacac
     3721 gaatgtgggt gatcgcgtag tggtacgtaa aatcgtgttg gccaagccga ccacggttta
     3781 caaaaaagag gaaacgacca caacaacgac gagagagcca taatcgcgaa aaggccgttc
     3841 gcggtagatt caaaagcgct cgcggaaact tttcgcgagc gcttttttct gtacttgctg
     3901 gtggggcgag actctgtcga gccgacgaaa tttgcctcaa attattccat cgctcagcgt
     3961 cgccttgcca gtttcaatca ccatagccgt ttggaaattt ctggtgccac ttccaggcac
     4021 tctcgatgat tgcgtcgagt gactggaact gcggttgcca gccgagttca tttttgatct
     4081 tgtctgaaga agcgatgagc cgcggtggat cgcccgggcg acgcggcctt tccacggtgt
     4141 cgatcttttt gccagtgact ttgcggcaag cggcgatcac atcacgaaca ctggcgccgc
     4201 cgccggtacc gagattgtag agttcgcttc tgggtgcgcc cagggccaga atgtgcgcac
     4261 gcgcaagatc gacaatgtgg atgtagtcac gaatgcaggt gccatccgga gtttcgtaat
     4321 cggtgccaaa aatctccacg tgtggctttt ggccaagcgc gactttgagc gtgtttggga
     4381 tcagatgcgt ttcgactcgg tgatcttcgc cgaaatttgc ggaagcgccg gccgcattga
     4441 aataacgcag tgaaacgaac ttcaaaccat gaatctcgtc gtaccagcgc agaatctttt
     4501 caaaggccag cttcgattcg ccgtatggat tgattggacg cctcggcgca gtttcgtcga
     4561 ttggcagccg ttccggcggt ccgaaaatcg cgcaggtcga ggaaaaaacg atccgctgga
     4621 cgcgtgccgc caccatcgca tccagcagat tgaggccgtt ggaaatgttg ttgcgaaaat
     4681 atttggatgg gtcgcgcatt gattcagaga ccagggcgct tgcagcgaaa tgcataaccg
     4741 cctccggctg ggccagggcc agcgcggaat cgatcgcctg tcgatcctgc aagtcgcctt
     4801 cgatgaatcg cgcgcgcgga tcgagggcgc tgcgatggcc ctcgcaaaga ttatcgaaga
     4861 ctgcaatctc gtggccttga tcaagaagca gctctgcgca gatgctaccg atgtaaccgg
     4921 ccccgccaac gacgagaatt ttcatccacc gtgaaaatgc ggcgtaattt gcactaaatc
     4981 aaagcgaggc ggctggtcga gctgtcgggg ggcgctcggc gacagcccct tccacactgg
     5041 cttcgagtca gagcgaagtg cgagcagaag aagcttgggc gacagttttg cctctttttt
     5101 tctcaagcga cactaccatc tgccgcgcgc aacgatgttt gcagcgcggt ttgagcacct
     5161 tcaagctctc ttcttccgat ccccatttca aaatggcgat gtccacatga cgcacgcccc
     5221 actttttcat ggcgaggcgg tttgtcttgt agagatcgat cgtgtcggta ccgatcagag
     5281 ccgtcccata atcgtcgata atgtactcct cgttggtatc ggcgattcgg aaatgtgtcc
     5341 cgagtgggaa gcgcgaccaa tcggacgcgg cgctcatcac gtggtggctg gaaaggcggt
     5401 cgccgagggc gttacgaggt ccgccttttt cggtatgcgt ataggcggtc gtccggacgc
     5461 gttgggtgcg ttgtgcgctc gtcaggtccg ttcttggctg cgtggcgcag ccaacgatta
     5521 aacaagcggc actggctgcc agtgtgaggt taaggcactt cgacattggt taaggcactg
     5581 cgacattggc aatatcgcca ctttccatat caactgaagc agcttcacgc aagaaataat
     5641 ttgccgcgcc aacatcacat cccgaaagct ttcgggctac aacatcaacg cgtggccgtt
     5701 tgcgccagtt ccgttgtgca atgcgggcaa cgcgtggctc tgatcggaat cgtcattgcg
     5761 cacgccgggc attcctttga cgcaggctct ttttgtgccg gtcccctcat cttgttgatt
     5821 ccgcgcacaa gtagaaaaat tgcgcctgcc acgatcagga aattgatcaa cagagtcatg
     5881 aagttgccgt aattccaagt gatcgcgcct gctttcaccg cgtctgccgg cgtattaaat
     5941 gccgtcgatc cgtctttggc ggctcgcaga atgatgaact gattcgagaa atcaagtccg
     6001 ccggtgagca ggctgatcgg aggcatgacg acgtctttga ccagcgaatt gacgatcgaa
     6061 ccgaaggctg cgccaattat cacacccact gcgagatcaa cagcgttacc tttgacggcg
     6121 aattccttga attcgttcca gatctttttc atacgacgat gttgactaat tttcccggga
     6181 cgacaacgat tttgcggata gtcttgccgg cagtcaattt ttgaatcttc gaatgcgcga
     6241 gcgcggcggt tttcgtttcc tcctccgtcg ccaggatcgg catcttcatt cgatcgcgca
     6301 cttttccgtt cacttgaatc accatttcca cttcgtcttc aaccaggaat cgttcatcgt
     6361 aatcgggcca ttgctggccg ctgatctcgc cggcgtcatc ccgaaacgtt gcgttcagct
     6421 tctcccaaag ctcggaagtg agatgcggcg caaatggatt gagcaaaacg agaaacctgc
     6481 gcatcgccgg caacggaagc gtttcggtgt tggtaaattc gttcacgaaa atcatcatct
     6541 gcgaaatcgc ggtattaaat gagaatgact cgatatcgtc ggtgactttc ttgatcgtgg
     6601 catgcgtcac cttctgttgc gccttatctg gctcgacatc tttgaccctg gctgacaatt
     6661 cccagtcgcc cgcctgattc tcggtcatga atagacgcca gacgcgcgca agaaaacgcg
     6721 ccactccttc gacgccccgc atgctccacg gtttcatttg ctcgagcggc cccataaaca
     6781 tctcatagca acggaacgcg tctgcgccgt actggtcgat cacagtgtcc ggattgacga
     6841 tgtttccgcg cgatttcgac attttctggt tgtcttcgcc gagaatgatt ccctgattga
     6901 ccagccgctg gaatggctcc ggcttcgaca aataacccag atcaaacaac accttgtgcc
     6961 agaaacgcgc gtaaagcaga tgaaggacgg catgttccgt tccaccaaca tacaagttca
     7021 ctccgcccgg ccgcttgctc aatgacgaat caatgttgga gtgaccagct tcgtcgcctg
     7081 cgcttcgggt ctcggatttc gtcattcctt cctcatgcgt cattggtgct gcgccatttt
     7141 cccctcccat ccaataacgc tccgcctctt tgccaacgaa ccgctcatcg ttctgcggat
     7201 cgcaaaatct taagtaatac cagcatgagc ctgcccattg cggcatggtg ttcgtctcgc
     7261 gcaccgcctt gtcagaatag cgaacccact ctttggctcg ggccaacggc ggctcgcccg
     7321 tgccagtggg cttgtaatct tcgagcggag gcggcatcac cggcaattct ttctcatcca
     7381 ccgcacgatg tttgcccttt tcccaaacaa tcgggaaggg ttcgccccaa taacgctgac
     7441 gcgagaacaa ccagtcacgt agcttgtagt tgatggcgcc tttgccaagc ccgcgttgtt
     7501 caagccattc ggttatcttg cgaatggctt cccgtgatgg cagaccgtta atgactggtg
     7561 aattggtcgc gattccttca ccgacaaatc caatcggatc ctggtcgcct ggcggctgca
     7621 cgacctctcg gatctgcaaa ttgaatttac gcgcgaactc gaggtcgcgt tcgtcatgcg
     7681 cgggcacgcc catgatcgcg ccggttccat aaccaggcag tacgtaatcg gcaacccaga
     7741 ttggaatccg ctcgttgttt accggattaa ttgcgtaggc gcccgtgaag acccctgttt
     7801 tttccttcgc cagttccgtt cgctccagat cgctcttgcg cgccgcacgc tcgcgatatt
     7861 cgcgcactgc cggccattgc tcttcgctta cgactagatc gataagtgaa tgctcgggag
     7921 cgaccaccat gtaagtggcg ccataaagcg tgtcggggcg ggtggtgaag acgcgaattt
     7981 tgtaaccgga gttggtggcc gcggcgccgg ggtcaccgac cccggctaca gcaaaatcga
     8041 tctccgcgcc ttcgcttttc ccgatccaat ttcgctggag cagtttgatc gattccggcc
     8101 aatcaagccc atcgagctcg tcgatcagac gctccgcata tgcggtgatg cgcagcatcc
     8161 attgccgcat cggtcgtcta acgacaggaa agccgccgac ctcgcttttc ccgtcgacaa
     8221 cttcttcgtt ggccaggacg gtgccgagtt ccggacacca gttcacggga aaatcggcga
     8281 cataagccag gcgaacgctg tccggatcgg tgccacgata ggtcgaaatc ggctcggctt
     8341 tatttgtgtc cggattaaac caggaattgt aaatctggag aaaaatccac tgcgtccatt
     8401 tgtagtagcg cggatcggtc gtgttgatct cccgctgcca atcgtaggaa aaaccgatcc
     8461 gttttagttg gcttttaaat ttcgccacgt tttcacgcgt cgtgattgcc ggatgctggc
     8521 cgctcttgat ggcgtattgc tccgccggca atccaaacgc gtcccaaccc atcggatgca
     8581 aaacgttgaa cccacgcatc cgtttgtatc gcgcgacgat atcggtggcg gtgtaacctt
     8641 caggatggcc gacgtgcagg ccagctccgc tgggataggg gaacatgtcg aggatgtaaa
     8701 atttcggcct tttcggatcg aaatgttttt cgccgggatt tgaggcgtga aaaagctgcc
     8761 gctcgtccca gattttttgc catttcggct cgatctgatc gaatggataa ggtttgcgac
     8821 gttcgttcat gaaaacccct gacgtttaac cacgaatgga cacgaacatt aaaatgacaa
     8881 aacccgaatg acgaatgacg aatcaatgac gaaacccgaa tgaggcaagg ctaaatgccg
     8941 attccatcgg gatttgccag ccgcgtcgga ttcgtgtcgt cgtggccgct cgattggaca
     9001 cttgtttatt ttgtcattca tcgtgcttcg tcattgtctc atctttgttc gtgtccattc
     9061 gggttgatta gtggttaatc cattgaatcc tgtcaaacgt gcagcatttg attgtcgcta
     9121 cgcgcaatgc gcataagacg cgcgagattc agcgaattct tggagcggac ttctccgtga
     9181 gcgatctatc ggcttatccc gagatttccg aaacacttga aagcggaaaa acctttgaag
     9241 aaaacgcgat actgaaagcg gtcaccgtgt cgggacaagt caagggacta gtcgtggcgg
     9301 atgactctgg attggaagtt gacgcgcttg gcggcgctcc gggaattcat tcggcgcgct
     9361 acgcaggcgc gaatgcgagc gacaaagaaa agatcgacaa gttactgagc gaacttggga
     9421 agattagtgc aaaaggcgat cagcctgttg cgcgatttcg ttgtgtgctc gctctcgcgc
     9481 gcgacggcca gattcttgga acgttcgaag gagttgtaga gggagaaatt gccggtcaag
     9541 cgcgcggctc gcatggattc ggctacgacc ctattttcct gccgagggga ttcggccgaa
     9601 ccttcggtga gtttgcaccc gcggaaaaag attggtggag tcacagggcc cgcgctttgg
     9661 aaaagttgcg cgcgtttctt aatactgtgc ccggctcagc aaagaaataa tcgggatttt
     9721 atcgtgccgc tgcggctctt aagccgagcg atcaaggctt gggcggt
//
LOCUS       DASQUQ010000193         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_700835, whole genome shotgun sequence.
ACCESSION   DASQUQ010000193 DASQUQ010000000
VERSION     DASQUQ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700835"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VE486_05565"
     CDS             complement(<1..390)
                     /locus_tag="VE486_05565"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZA38587.1"
                     /translation="MIVSPMMGGRQWKLGDRVFDVSRQGLIMGILNVTPDSFSDGGKF
                     FTAEKAVEHGLRMTAEGAQIIDVGGESTRPGAESVAVEEELNRVIPVIEKLRPRNDIV
                     ISIDTSKAQVAREAIQAGASVVNDVTGG"
     gene            complement(466..984)
                     /locus_tag="VE486_05570"
     CDS             complement(466..984)
                     /locus_tag="VE486_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZA38588.1"
                     /translation="MQKAIIAGAFAIALAAMASFAAGGDKMKITSSAFQEGANIPSKF
                     TCDGADTSPPLEIADIPSEAKSLALIVDDPDAPSGLFTHWAVWNISPQTSAVREGSAP
                     NGKQGTNDFGKSGYGGPCPPSGTHRYYFKIFALDRELDLPFGAKRGQLDAAMKGHVVA
                     QGELMGRYSKQK"
     gene            1049..3238
                     /gene="rnr"
                     /locus_tag="VE486_05575"
     CDS             1049..3238
                     /gene="rnr"
                     /locus_tag="VE486_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZA38589.1"
                     /translation="MASNGRKIQEQILALLGRKDYRPLDKNGLAKTLGRKSGVRMGLN
                     QALRELERAGEIARIRKNRYVLPAEADLVAGKVSIHQAGYGFLTPEKPGEPDIFIATE
                     NTGTAMHGDRVVARISQDAPYGRIRGRREGRVIRILERAHDTIVGTLQHSRNFYYVVP
                     DDPRLVHDVYVRPALDQRSQTAATVGDKVVVRLEAWESRHVNPEGEIIEVLGPASAPG
                     IDMLSIIRKYHLPTEFPRDVSDQADQISEKIDARQLEGREDLRKEFIVTIDPDDARDF
                     DDAIHVKKIENSGWHLGVHIADVAAYVEPESALDREARRRGNSVYLPDRVIPMLPERL
                     SNGVCSLNPGVDRLTHSVFIHFNKHGMATSARFARSVIRSAHRLTYKQAYAILKSAPR
                     DQLGERLHVAWELASLLRRKRFEDGSLDLDFPEVKVWLDKNGYPAKLERVENDESHQL
                     IEECMLAANEAVARELNKRGVPTIYRVHENPDPEKLAEYREFVLSFNYRVGDLTQRGE
                     IQRLLAAIRGKPEEQALKIGLLKSLKRARYLPQPLGHYGLAKSNYLHFTSPIRRYADL
                     VVHRVLVERDGKHDSRIGMSQVASIAEHISTTERVAADAEIESVRMKKLEFFQRQLDE
                     RNPQIFRAAIVDVRNYGLAVELPDALITGLVHVSSLADDFYSFEPAQRRLIGRRSRKR
                     FSIGDVVRVFVVRVDVFKRQVDFAIASEAPMKRGHGKRHSRVPSSSG"
BASE COUNT          815 a          989 c          976 g          788 t
ORIGIN      
        1 tcctccagtg acgtcgttta caaccgaagc cccggcttga atcgcctcgc gcgcaacctg
       61 cgccttcgaa gtgtcgatcg aaataacaat gtcgttcctg ggacgtaatt tttcgatcac
      121 cggaatcacg cggttcagtt cttcttctac cgcgacagat tcggcgccgg gtcgcgtcga
      181 ttcgccacca acatcaatga tttgtgcgcc ttccgccgtc attctcagcc cgtgctcgac
      241 tgccttttcc gccgtgaaaa atttcccgcc gtcggagaat gagtccggag taacgttcaa
      301 aatgcccatg ataagtccct gccgggagac atcgaaaacg cggtcgccga gtttccactg
      361 cctcccaccc atcatgggtg agactatcac aggacgcgga ttattcgaag gaagagcgag
      421 gagaagaaat gactgacgaa tgtcgaatta tccaggaatg ctccgtcatt tttgctttga
      481 gtagcgcccc attagctcgc cttgggcgac gacatgacct ttcatcgctg catccagttg
      541 gccgcgtttt gcgccgaatg gcaggtccag ctcgcgatcg agcgcgaaaa tcttgaagta
      601 ataacgatgt gtccccgatg gcggacaggg gccaccgtaa cccgatttgc cgaagtcatt
      661 tgtgccctgt ttgccattcg gggcactgcc ttctcgcacc gcgctggttt gcggcgagat
      721 attccaaacc gcccagtgcg tgaacaatcc acttggcgca tccggatcat caacaatcaa
      781 ggcgaggctt tttgcttcgg aagggatatc cgcaatttcc aacggcgggc tggtgtccgc
      841 gccgtcgcac gtaaattttg atggaatgtt tgcgccttct tgaaaagcag aacttgtgat
      901 cttcatcttg tctcctcctg cggcgaacga agccattgcc gccagtgcaa tcgcaaatgc
      961 gccggcgata attgcttttt gcattactcg actctccggt tgaaatggcg ttacatcgac
     1021 agtaactcga cgacattgtt tcgcagcgat ggcttcaaac ggacgtaaga ttcaggagca
     1081 aattctcgcc cttctgggcc ggaaagatta ccggccactc gataagaatg ggctggcgaa
     1141 aacgctcggc cgaaagagcg gcgtgcgcat gggcttaaat caggcgttgc gcgagttgga
     1201 acgcgctggt gagatcgcgc ggatccgcaa aaatcgatat gtcctgccag ctgaggcgga
     1261 tcttgttgcc ggaaaggtat cgattcatca agccggatac ggttttctca ctccagaaaa
     1321 accgggcgag ccggatattt tcatcgcgac ggaaaacacc ggcaccgcca tgcacggtga
     1381 ccgggtagtt gctcgcatct cgcaggatgc gccctatggc cgcatcagag gacgacgcga
     1441 aggacgcgta attcgaattc tggagcgcgc acacgataca atcgttggga cgctgcaaca
     1501 ttcgcgcaat ttttattacg ttgtcccgga tgatccacga ctcgtccacg acgtttacgt
     1561 gcgccctgcc ctggatcagc ggtcacagac cgccgctaca gttggcgaca aggtcgtcgt
     1621 gcggcttgag gcctgggaat cgcgtcacgt caatccagaa ggcgaaatca tcgaagtgct
     1681 cgggccggcg tcagcgccgg gcatcgacat gctgtcgatc atccggaaat atcatctgcc
     1741 gacagaattt ccgagagacg tttcggatca ggcggatcag atttccgaaa agatcgatgc
     1801 acggcaactc gagggacggg aggatttgcg caaagaattt atcgtcacca tcgatccgga
     1861 tgacgcacgc gatttcgatg acgcgattca cgtcaaaaag atcgagaata gcggttggca
     1921 tctcggcgtt catattgccg atgtcgccgc gtacgtagaa ccggagagcg cactcgaccg
     1981 cgaagcgcgc cggcgcggaa acagcgttta tctgccggac cgcgtgattc ccatgctgcc
     2041 ggagcgatta agtaacggcg tgtgcagtct caacccggga gtcgatcggc tcacgcactc
     2101 ggtgtttatt catttcaata aacatggaat ggcgacaagc gcgcgctttg cccgaagcgt
     2161 gattcgaagc gcgcaccggc ttacctacaa acaagcgtac gcgattttga aatccgcgcc
     2221 tcgcgatcaa ttgggggagc gactgcacgt tgcctgggaa cttgcctcac tattgcggcg
     2281 aaagcgtttc gaagatggct cgcttgacct tgattttccg gaggtaaaag tgtggctcga
     2341 taagaatggg tatccagcga aactcgagcg ggtcgaaaat gatgaatccc atcaattgat
     2401 tgaggaatgc atgctcgccg caaacgaggc cgtcgcgcgc gagctgaaca aacgcggtgt
     2461 tcccacgatc tatcgcgtcc acgaaaatcc cgatccggaa aaactggcgg agtatcgcga
     2521 attcgttctc agttttaact acagggtcgg tgatctgacc cagcgcggcg agatacagcg
     2581 tttgcttgcc gcgatccgcg gaaaaccaga agaacaggcg cttaagatcg gtttactgaa
     2641 aagtctgaaa cgggcgcgct accttccgca gccgctcggt cattatggac tggctaaatc
     2701 caattacttg catttcacga gtccgattcg ccgttatgcc gatctggtcg tgcatcgcgt
     2761 tttggtagag cgcgatggga aacacgactc ccggatcggt atgagccaag ttgcatcgat
     2821 cgcagagcat atttcaacga ccgagcgcgt cgccgccgat gcggaaatcg aatccgttcg
     2881 aatgaagaag ctcgagtttt tccaacgaca gctcgatgaa cgcaacccac agattttccg
     2941 cgctgcaatt gtcgatgttc gcaattacgg tctcgcggtt gaattaccag acgctctgat
     3001 tacgggtttg gtgcacgtat cctcgttggc ggacgatttt tattcattcg agccagctca
     3061 acggcggctc attggcagac gatcgcgcaa acgattcagc ataggcgatg tagtccgcgt
     3121 cttcgttgtg cgggtcgatg tcttcaaacg gcaggtcgat ttcgcgatcg cttcggaagc
     3181 gccaatgaaa cgaggccacg gcaaacgcca ttcgcgcgtt ccttcttcct ccggctaaaa
     3241 ccgttcctcc caaacggttg ccattacgga caaattagca aacagcccga gaacgcgctc
     3301 ggacctggta ggtggacacg agctgcgcgt tgtgccgtct ctgtctcgag gaggcgccga
     3361 atcttttgac ttataaccgc gacgagactg gcgttcattt tttcaaacaa cccgaaacgc
     3421 cagatgaaat cgcggccgct caacgcggca tggatgtttg cccggcgctc gcaatcggaa
     3481 atgacggcta ataacttgcg acgatcagag atcgccgcta caaacccgct atgcctgctg
     3541 aagccactgt cgccgctccg ccatactc
//
LOCUS       DASQUQ010000194         6347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_701353, whole genome shotgun sequence.
ACCESSION   DASQUQ010000194 DASQUQ010000000
VERSION     DASQUQ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701353"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1392)
                     /locus_tag="VE486_05580"
     CDS             complement(247..1392)
                     /locus_tag="VE486_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HZA38590.1"
                     /translation="MTKPSTFSIRQSPAAPNPATAGECGSFSTGLIATDAQLVELLNK
                     IDPAERVAIDTEADSLHCYREKLCLVQLSLPTSNCIVDPLADVDLAPLRLALERTEIV
                     LHGADYDLRMLRRGLYFVARKIFDTMIAARLLGIREFSLAALVKRYFGLELPKGSQKA
                     NWAQRPLPARMAEYAINDVRYLLPLAEELEAELDRYGRLDWLRQSCQRAIEQAGVQRV
                     RDEDELWRISGSGSLRGREAAILRALWQWREKEAEAADRPPFHVLQNDQLLNAAAAFA
                     SGDTPDYRHFSLRRRQAFYQAAQGAMQLPESEWPVSRRRVGRRPGSENVRAMEELRRR
                     RDQAANELNLEPAFIAPRNTLEAIATSETRAASLLVPWQRELLGIRA"
     gene            complement(1399..2766)
                     /gene="rho"
                     /locus_tag="VE486_05585"
     CDS             complement(1399..2766)
                     /gene="rho"
                     /locus_tag="VE486_05585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZA38591.1"
                     /translation="MATAIMDEKLEASASLEADTVDGDVGHEPGSTPSATDRSLDLNE
                     LQGLPDKKLKALARDLDLHLHPARSRHQHILDVIRAALSTGGMVTAEGFLDQVSDSFA
                     MLRWPKLNFLAVPEDVCVPRAVIEQYHLRPGQKIAGTIRLPGHREKFLTLEKLATIEG
                     QPAEQWVERSDFDKLTPQFPQGRIMLENPKTNSISARAVDLLAPLGRGQRGLIVAPPR
                     VGKTILLKEIAKAIRVNHPEIVLILLLVDERPEEVTDLEREIDCQIYHSNFDENVHRH
                     VQVAEMVLERSKRLVEMKQDVVVLLDSITRLSRGYNSLQPGRGRIMSGGVEAKALTKP
                     KKFFGSARNVEEGGSLTVLATALTETGSRMDELIFEEFKGTGNMELHLDRALQEKRLY
                     PAIHPLLSATRREDLLYHPDEWERVQNLRKTMAALPPIEAMEKLIENLEATKTNAELL
                     LSGLR"
     gene            complement(2736..3350)
                     /gene="coaE"
                     /locus_tag="VE486_05590"
     CDS             complement(2736..3350)
                     /gene="coaE"
                     /locus_tag="VE486_05590"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZA38592.1"
                     /translation="MPAIGITGGISTGKTSFCQCLREILPAAKFFDADQEAHRLADLD
                     PEVKEEIRREFGTGIFSADGDLNREQLRAIVFGDAAKKRALEQILHPRIRRQWTAEAE
                     THRNSPGFFFADIPLLYETGGETLCDQVVVVACSYPTQLRWLMERMSLEPAAAEQMIN
                     SQMSLEEKIRRADHVVWNNGPRAPLAEQARLLVALWQQQSWTKS"
     gene            3539..4315
                     /locus_tag="VE486_05595"
     CDS             3539..4315
                     /locus_tag="VE486_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HZA38593.1"
                     /translation="MTPQVLAGKIGVVFGVANKRSIAWAIAKAWAAAGARLIFNYQGE
                     RLKENVEELAGEFGEKTPLFPCDVSSDEQIRAFFDSVRGQTDRLDLMLHSLAYAPKEA
                     LEGDFVSTTREAFRTAHDISAYSLVALARESASLMTNGGSILAMTYYGSEKVVPHYNV
                     MGVAKASLEASARYLAYDLGPKKIRVNCISAGPVNTLAARGIAGFTSMLKHYQERAPL
                     KRSCDPAELGETGVFLASDGAAAITGQVIYVDGGYQIMGM"
     gene            4368..5738
                     /locus_tag="VE486_05600"
     CDS             4368..5738
                     /locus_tag="VE486_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZA38594.1"
                     /translation="MDLKQKLVELLGVHAVSNESETLAAHSGDKWFAAHAPEAVVFAR
                     STSDVSKLLQFASREKIPVTARGGGFGYVGGCVPVRGGIVLSLIRMNRIMEISFADAV
                     AVVQPGVFTADLKAAARAEKLFYPPDPASMKDCTIGGNVATNAGGPRCLKYGVTRNYV
                     IGLEVVLADGEVLRTGGRVHKNKTGFDLVGLFVGSEGMLGVVTEITLRLLPLPPARAT
                     LSAAFATAAQSAEAVQAIFAAGFLPSSLEIADHFTLEAARRDLGEMIVPAGNAHLLVD
                     LDGQERSVRSEAAAIRELLETRKANAVEMAIGEADCERLWALRREFSNSLRATGLTKL
                     NQDVVVPRSRLVDLMEFAEKLQIKHGFPIACFGHAGDGNIHVNIMADRYNRDASVRAK
                     VDSALDELFKQVLAWGGVITGEHGIGLAKKRWWPEATSAVVRDVHQKLKQILDPDGIL
                     NPGKFV"
     gene            6058..>6347
                     /locus_tag="VE486_05605"
     CDS             6058..>6347
                     /locus_tag="VE486_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38595.1"
                     /translation="MKPGTIAHTIAVILSSISSLFAHGLPSKAEAPKVKASKKSNRSS
                     PLERGLLDVGSIAFSPGQLSATSPKPGERFPWKKSIVTTVFWIGEKPSENNPV"
BASE COUNT         1388 a         1750 c         1782 g         1427 t
ORIGIN      
        1 ggaacgcggt ctacgacagc gcgcactttt tccgagtctc cagtcatggc cagaactact
       61 acggtcacat gcccatcagt ggtggaaagt ttgtggccat caatatcgac aaaattgagc
      121 gcgtagcgtt gcccgttcga cagcatcccg tgcaaagagg ccgcgagaag gcaagacacg
      181 cccgttgcac agaaaaacga ctgaagtgcg cgcacgctca caaggcagcc aatttaccac
      241 agagcttcac gctcttattc cgagcagctc ccgctgccac ggaaccagca aggatgccgc
      301 gcgcgtttcg cttgtagcga tcgcctcgag tgtgttgcgc ggtgcgataa aggccggctc
      361 gagattgagt tcgttcgcag cctgatccct gcggcgccgc agttcttcca tcgcgcgcac
      421 gttttctgaa ccgggtcgcc tgccgacgcg acgccgtgaa accggccatt cagactcagg
      481 caattgcatt gcgccttgtg ctgcctggta aaatgcctga cggcgacgca aggaaaaatg
      541 cctgtaatca ggagtgtctc cggaggcgaa ggccgccgct gcgtttagta attgatcgtt
      601 ttgcaaaaca tgaaaaggcg gtcgatctgc cgcttccgct tctttctcgc gccattgcca
      661 aagcgcgcgc agaatggctg cttcacggcc gcgcaacgag ccagagccgg aaatgcgcca
      721 aagttcatcc tcgtcgcgga cacgttgcac gcccgcctgc tcgattgcgc gctggcacga
      781 ttgtcgcaac caatcgagac gaccatatcg atctaactct gcctccaatt cttcggcgag
      841 cggcaacagg taacggacat cgttgatcgc gtactccgcc atccgcgccg gtagcggccg
      901 ctgcgcccaa tttgctttct gtgaaccttt cggaagctcg aggccgaagt aacgtttcac
      961 cagcgcagcc agactgaact cgcggatgcc gagcagccga gccgcaatca tggtgtcgaa
     1021 aattttgcgg gcaacgaaat acaaaccgcg ccgcaacatt cgcagatcgt aatcggcgcc
     1081 gtgcaaaaca atttctgtgc gttcaagggc caatcgcaat ggtgcaagat cgacgtccgc
     1141 cagtggatcg acgatgcaat tactcgtcgg caaactaagc tgcaccagac aaagcttttc
     1201 tcgataacaa tggagactgt cggcttccgt atcgattgca acccgctcgg ccggatcaat
     1261 tttgttgagt aactcgacta gctgggcatc agtcgcaatg aggccggttg aaaatgaccc
     1321 gcattctccc gcagtcgcgg gattcggcgc ggcaggcgac tgacgaatgg aaaatgtcga
     1381 aggcttcgtc atcgcccgtc acctcaaccc gctcaagagc agctccgcgt ttgttttcgt
     1441 cgcttcgagg ttctcgatca gcttttccat agcctcgata ggtggcagcg ccgccatggt
     1501 cttgcgcaga ttctgcacgc gctcccattc atcgggatga tagagcaaat cctcgcgccg
     1561 cgtggccgag agcaatggat gaatcgccgg gtagagccgc ttttcctgca atgcgcgatc
     1621 aagatgcagc tccatgttgc cagtgccctt gaattcttcg aagatgagtt cgtccatgcg
     1681 gctgcctgtc tcggtcaaag cggtcgcgag cacggtcagg ctgccgcctt cttcaacatt
     1741 gcgggcggag ccgaaaaatt tctttggttt ggtgagtgct ttcgcctcga cgccgcccga
     1801 catgatccgg cccctgcccg gctgcaaact gttatagccg cgcgagagcc gcgtgatgct
     1861 gtcgagcaaa accacgacgt cttgtttcat ttcgacgaga cgtttggagc gctccagcac
     1921 catctccgcc acctgcacat gtcgatggac gttttcatcg aagttcgaat gataaatctg
     1981 acaatcgatt tctcgctcga gatcggtcac ctcttccggc cgttcgtcca ccaacagcag
     2041 aatgagaacg atctccggat gatttacccg aatcgctttg gcaatttcct tgagcaagat
     2101 cgtcttaccc actcgcggcg gcgccacgat cagaccgcgc tgtcctcgcc cgagcggcgc
     2161 cagcaaatca accgcccgcg cgctgatcga attcgtcttt ggattttcca gcatgatccg
     2221 gccttgcgga aattgcggag tcaatttgtc gaaatcgctc cgctccaccc attgctcggc
     2281 cggctggccc tcgatcgttg cgagcttttc aagcgtgaga aatttctcgc gatggcccgg
     2341 aagccgtatc gttccggcaa tcttttggcc cggccgcaga tgatattgct cgatcaccgc
     2401 gcgcggaacg cacacatcct cgggcaccgc caaaaagttc aatttcggcc aacgcagcat
     2461 tgcgaacgaa tcgctcacct gatcaagaaa cccttccgcc gtaaccattc cccccgtaga
     2521 taatgcggcg cggattacat cgagaatgtg ctgatgacgc gaccgcgccg gatgcaaatg
     2581 cagatccaaa tcccgcgcca acgctttcag ctttttatcg ggaagccctt gcagctcgtt
     2641 taagtcaagc gagcgatctg tggccgaggg cgttgacccc ggctcgtggc caacatcccc
     2701 gtcaaccgta tctgcttcca atgaagctga ggcttctaac ttttcgtcca tgattgctgt
     2761 tgccacagcg caacgaggag gcgcgcctgc tcggcaagtg gcgcacgagg tccattattc
     2821 cagactacgt ggtctgcacg cctgattttt tcctcgagtg acatttgtga gttgatcatt
     2881 tgttcagccg cggcgggttc gagcgacatg cgctccatca gccagcgtaa ttgagttggg
     2941 taagaacagg cgaccaccac gacttgatcg cacaatgttt caccgcccgt ttcataaaga
     3001 agtggaatat cagcgaagaa aaaaccggga gagttgcgat gcgtttccgc ttctgcggtc
     3061 cattggcggc gaatccgcgg atggagaatc tgttcgagcg cgcgtttttt tgccgcgtcg
     3121 ccgaatacta tggcgcgaag ttgttctcga ttcaagtcac catcggctga aaaaatccct
     3181 gttccaaatt cacgacgaat ttcttcttta acttctggat caagatcggc gagccggtgt
     3241 gcctcctgat cggcgtcgaa aaatttggcg gctggaagga tctcgcgcag acattggcaa
     3301 aagctggtct tgcctgtgga aattccgccg gtgatcccga tggcaggcat ttaagcaagg
     3361 attaaaaggc tgtagaggtg gctgtgtcag ccacaaaata ttgcaaaagc cgccacgcga
     3421 cgaagggcga cggtcaattt cccaacgatg gtcgtccttg ccgaaccttg ttggtatcgg
     3481 tccatgattt ctaattgcgg tttttgcggc cggttaaagc tgggtatctt cccgatctat
     3541 gactccgcaa gttttggcag gaaagatcgg tgttgttttt ggcgtggcga acaagcgcag
     3601 catcgcctgg gcgatagcga aagcctgggc tgcggccggt gcgcgtttga tttttaatta
     3661 tcaaggcgag cggttaaaag aaaacgtcga ggaattggcc ggcgaatttg gggagaaaac
     3721 gcccttattt ccctgtgacg tttcgagcga tgagcagatc agagcatttt ttgacagcgt
     3781 gcgcggccaa actgaccgcc ttgatttgat gcttcactcg cttgcgtacg cgccgaaaga
     3841 agcgctggaa ggcgattttg tgagcacaac acgcgaagca ttccgcaccg cgcacgacat
     3901 cagcgcttac tcgctcgtcg cgctcgcacg tgagtccgcg tcgctgatga ccaatggcgg
     3961 gagtattctg gcgatgacgt attacggctc ggagaaagtc gtgccgcact acaatgtcat
     4021 gggggtggcg aaggccagcc ttgaggcgag cgcgcgctat ctcgcttacg atctcggccc
     4081 gaagaaaatt cgggtgaact gcatttccgc tggcccagtg aatacgctgg ccgcacgcgg
     4141 catcgccggg ttcacgtcga tgttaaagca ttatcaggaa cgcgcgccgt taaaacgcag
     4201 ttgcgacccg gcggaactcg gcgagaccgg agtatttctt gccagcgatg gcgcggcggc
     4261 catcacaggc caggtcattt acgtcgatgg cggttaccaa attatgggaa tgtgagatga
     4321 tttcagattt tcgatttttg attttcgatt tgaggcgaac ctgatcagtg gatctgaaac
     4381 aaaagctcgt agagctgctt ggagttcatg cggtaagcaa tgaatccgaa accctcgctg
     4441 cgcatagcgg cgacaaatgg ttcgcggcgc acgcgccgga ggcagtggtt tttgccagat
     4501 cgacgagtga cgtcagcaaa ttgcttcagt ttgcgtcgcg ggagaagatt ccggtaacgg
     4561 cgcgaggcgg cgggttcggc tatgtcggcg gatgcgtgcc ggtgcgaggc gggatcgtct
     4621 tgtcgctcat ccggatgaat cgcatcatgg aaatcagctt tgccgatgcg gtcgcggttg
     4681 ttcaaccggg cgtgtttacg gcagacctga aagcggcagc gcgcgcggag aaattgtttt
     4741 atccgcctga tccggcgagc atgaaagatt gcacgatcgg tggaaacgtc gccacgaatg
     4801 ccggtggccc acgctgtttg aaatacgggg tgacacgcaa ttacgtgatc ggcctagaag
     4861 tcgtgctcgc cgatggtgag gtgctgcgga caggcgggcg tgtccataag aacaagaccg
     4921 gatttgattt ggttggcctc tttgttggct cggaaggaat gctcggcgta gtcacggaaa
     4981 tcacgctgcg tttgctccca cttcctccag cgcgcgccac tctgtctgcg gccttcgcca
     5041 ccgccgcgca gtccgctgaa gcagtccagg cgatttttgc cgcgggattc ctgccttctt
     5101 cattggaaat cgccgatcat ttcaccctgg aggcggcgcg gcgggatctc ggcgagatga
     5161 ttgtgccggc gggaaacgct cacttgctcg tcgatctcga cggtcaggag cgaagcgttc
     5221 gcagcgaagc cgcagccatt cgcgaattac ttgaaacgag aaaagcaaat gccgtcgaga
     5281 tggcgatcgg tgaagcagac tgcgaaaggc tttgggcctt gcgccgcgaa ttcagcaatt
     5341 cacttcgcgc cacaggcctg accaagttaa atcaggatgt ggttgtgccg cgcagccgcc
     5401 tcgtagatct gatggaattc gccgagaagc tgcagatcaa acacggattt ccgatcgcct
     5461 gcttcggcca cgccggtgac ggcaatattc acgtcaatat catggcggac cgttacaacc
     5521 gagacgcttc ggtgcgggca aaagtcgatt ccgcgctcga tgaactgttc aagcaagtgc
     5581 ttgcatgggg cggtgtgatc acgggcgagc acggaatcgg cctcgcaaaa aagcgctggt
     5641 ggccagaagc gaccagcgcc gtcgtgcgtg atgtgcatca gaagctgaag caaattctcg
     5701 atccagacgg aattttaaat cccggaaagt ttgtttgacg ccgataaaac aatgacgaga
     5761 gaatgacata tccgaatgcc gtgaaaaata tgcagcactt ctttgtcatt taatcattta
     5821 ggcttccttc gtcattcgtc atttgtgctt cgtcattcga attttccgtg tgcattcgcg
     5881 ttattcgcgg gttgatcttt ttcaaggttg actaatccga atctgcattt gaggttgggc
     5941 gacatcatca ggcgcatctg aattcgccgc gactgacgcg catccgcgtc gtcgtaaccg
     6001 aatcaattaa agaagacgtg atgtcttgct tttgctgttg cgcagacccg atacgaaatg
     6061 aaaccaggaa ccattgctca caccatcgcg gtgatacttt cgtccatttc gtctctcttt
     6121 gctcacgggc tgccttcaaa ggccgaagcc cccaaggtga aggcgagcaa gaaatcgaat
     6181 agatcatccc cgctagagcg gggtctgctc gatgtcggtt caattgcgtt ctctccggga
     6241 cagctatcgg caacttcgcc caaaccagga gagcgattcc cgtggaaaaa gagtattgtg
     6301 acaacggttt tttggatcgg cgaaaaaccg agcgaaaata atccggt
//
LOCUS       DASQUQ010000195         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_701437, whole genome shotgun sequence.
ACCESSION   DASQUQ010000195 DASQUQ010000000
VERSION     DASQUQ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701437"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..367)
                     /locus_tag="VE486_05610"
     CDS             complement(83..367)
                     /locus_tag="VE486_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HZA38596.1"
                     /translation="MENEILYRRAAVRAAMQIAFYRHVMIYIIVNALLIAVNLLTDPH
                     HLWFQWGLLGWGIGLLVHGLNVYSYRWWGSRREQMIQRELERQRKNSPQS"
     gene            complement(512..1222)
                     /locus_tag="VE486_05615"
     CDS             complement(512..1222)
                     /locus_tag="VE486_05615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZA38597.1"
                     /translation="MASAATRVFMVRHGATVVSAEDRFAGATDVALSDEGREQIRRLA
                     ERLSHEKIAAVYASPLGRTVETAQILAAPYRLEVQTRDGLREISHGRWEQMTRREVEE
                     KFPEEAAEWEKDPYTFAPVGGESGLAVTARALPVLVELVRKHPGENILVVSHKATIRL
                     LLSSLLGFDPRRYRDNLDQKPAALNIVDFRDATRARLTLFNDTSHCAKAGEAVPPIPE
                     QRLSKWWSERAARAGEKR"
     gene            1318..>2054
                     /gene="dinB"
                     /locus_tag="VE486_05620"
     CDS             1318..>2054
                     /gene="dinB"
                     /locus_tag="VE486_05620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZA38598.1"
                     /translation="MEKNAKPRAIIHLDMDCFYAAIEVRDRPSLRGKPVGVGGARDRR
                     GVLTTCNYEARKFGVRSAMPTFMALQRCPDLIVLPTRFDVYRREAAVIRGILHRFTSL
                     IEPLSLDEAYLDVTEHPGAPGPLAQVIRGTIFRKTKLTSSAGIGPNKLIAKIASEINK
                     PNGQFEVTPEQVSGFMEKLPVRKIWGIGEKTGKKLAELGVNTCGELQRFSRPELVDLF
                     GKFGVELYDLCRGIDHRPVEPDRPRKSL"
BASE COUNT          440 a          624 c          554 g          436 t
ORIGIN      
        1 agcgcgaaat tgggcaatcc gtacgtctcg atctgctgca gaggcagccg cgtcggctac
       61 gaccgtgcga ctcgcggtgc catcaagatt gcggcgaatt cttccgttgc cgttccagtt
      121 cgcgttggat catctgctcc cggcgcgatc cccaccagcg atacgaatac acgttcagcc
      181 catgcacgag cagtccgatg ccccaaccaa gcagccccca ttgaaaccag agatggtgcg
      241 gatctgtcaa aagattaacc gcaataagca gcgcgttgac gattatgtaa atcatcacgt
      301 gacggtaaaa tgcgatttgc atcgcagccc gcacagccgc tctgcgataa agaatctcgt
      361 tttccatggt tttatttcag cacggctatc ccacgatggc gagcgcgatt tccggggctg
      421 atgtcccgga tctatcgcga ataaccgtcc gcttcgacca aaacgagacc cgtttcccct
      481 tgcgcagaaa ggcaacacgc cgcgcaaccc cctatcgctt ctcacccgcg cgcgctgcgc
      541 gctcgctcca ccacttcgac aatcgctgct cgggaatcgg cgggactgct tcaccggcct
      601 tggcgcaatg tgaggtatcg ttaaatagcg taagccgggc acgcgtcgcg tccctaaaat
      661 cgacaatgtt cagcgcggcc ggtttttgat cgagattgtc gcggtaacgc cgcggatcaa
      721 accccagtaa cgagctcagc aatagtcgga tcgtcgcctt atgcgaaacg acgaggatgt
      781 tttcgccggg atgcttgcgc accagctcga cgagcaccgg cagggcacgc gccgtcacgg
      841 ccagaccgct ttcaccgccc acgggcgcaa atgtgtacgg atccttttcc cattccgcag
      901 cttcctccgg aaatttttct tctacttcgc gtcgcgtcat ctgttcccag cggccgtgcg
      961 aaatctcgcg cagaccgtcg cgcgtctgca cctctagcct atacggtgcg gcaagaattt
     1021 gagcggtctc gactgtgcgt ccaagcggcg atgcataaac cgcggcaatc ttctcgtgac
     1081 tcaatcgctc cgccaaccgg cgtatttgct cgcgtccttc atcggaaagc gccacatcgg
     1141 tcgcgccggc aaatcgatct tccgcgctca cgacagtagc gccatgtcgc accatgaaaa
     1201 cacgcgttgc cgccgatgcc atacgatcag cttcgtgatg acgtaatttc tgccaaaacg
     1261 caatctctct tcgctgatca aagatgatag cgcgaccatt gaggttacat ttggccggtg
     1321 gaaaaaaatg caaaaccgcg cgccatcatc catctggaca tggattgttt ttatgcggcg
     1381 atcgaggtgc gcgaccgacc ttcgctccgc ggcaaaccag taggcgtggg cggggcgcgc
     1441 gatcggcgcg gcgttttgac gacgtgcaat tacgaagcgc gcaagttcgg tgtgcgttcg
     1501 gccatgccga ccttcatggc gttgcaacgc tgtccggact tgatcgtttt gccgactcgc
     1561 ttcgatgttt atcgccgcga agcggccgtc atccgcggaa ttttgcatcg gtttacgtcg
     1621 ttgattgagc cactctcgct cgacgaagct tatctcgatg tcactgagca tcccggcgcg
     1681 ccagggcccc tggcgcaggt aatccgcgga acgatttttc gcaaaacgaa gctcacctcg
     1741 tcggcgggaa tcggtcccaa taagttgatc gcgaaaatcg cgagcgaaat taacaagcca
     1801 aacggccaat tcgaagtcac gccggaacaa gtatccggct tcatggagaa attaccggtc
     1861 cgcaaaattt ggggaatcgg cgagaagacg gggaaaaaac tggcagaact cggagttaac
     1921 acctgcggcg aactgcagcg cttttcgcgt ccggaacttg tcgatctttt cggcaagttt
     1981 ggcgtcgagc tctatgatct ttgccgcggg atcgatcacc ggccagtcga gccggatcgt
     2041 ccgcgcaaat cact
//
LOCUS       DASQUQ010000196         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1327912, whole genome shotgun sequence.
ACCESSION   DASQUQ010000196 DASQUQ010000000
VERSION     DASQUQ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327912"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..525)
                     /locus_tag="VE486_05625"
     CDS             complement(109..525)
                     /locus_tag="VE486_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZA38599.1"
                     /translation="MRRYSQRTGLTTLSEINITPLLDLAFVLLIIFMITTPLLENSMN
                     LSIPSSGATNQPINSSQVQTVSIDRDETLRFNNQVVDLETLTTQLVQLKKANPDVAVV
                     IRPDRDLPVQKLIGLMDAVQRAGISKVGIATKAETK"
     gene            complement(638..1498)
                     /locus_tag="VE486_05630"
     CDS             complement(638..1498)
                     /locus_tag="VE486_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZA38600.1"
                     /translation="MSIRASFVIRHPCFVIGILLSFLQNGAITYAGDAEMLIATLLAA
                     GGLLFAFDHATIAGKLVLSLLAVASIFSWSIMVTKLRVIRFARQQNARFLAAFRQDRQ
                     PLRLFEKNARFPGSPVFNVYRAGCEEMTFHLLGSPEVDDTFRARLGIADKISPAQMGA
                     VSAAMERAVGETALALESQMILLATAVSGSPFLGLLGTVWGVMDAFTGVAEAGSPSLV
                     SMAPGVSGALITTVTALCVAIPAMFGYNFLVTSIRGIIVEMDNFAAELASEFEHKYVD
                     HSSRESAFRK"
     gene            complement(1561..2499)
                     /gene="lgt"
                     /locus_tag="VE486_05635"
     CDS             complement(1561..2499)
                     /gene="lgt"
                     /locus_tag="VE486_05635"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZA38601.1"
                     /translation="MLAYYLHNLDPIIFRIYDNVGPRWYGLAYVLAFICSYLLLRVLA
                     KTGYADLPVEKVGDFITGCALFGVIIGGRLGYVFFYKPEMLREPLSIVRVWEGGMSSH
                     GGMLGVLLFTFYYAHRHKISWLNLGDNLVVTAPLGLFFGRCANFINGELYGRITNVPW
                     AMQFPKELLDHPAEAERAIRTCAQIDPALTTPEAIIAAVNHQPQVASALRSILTPRHP
                     SQLYEAFFEGIVLFAILWLVRTRTRQPNGVLTGLFFIFYAIFRIATEFFREPDAAMIG
                     AFTRGQFFSFFLIAIGITFVVAARMRPTFPKKMTND"
     gene            complement(2509..2886)
                     /locus_tag="VE486_05640"
     CDS             complement(2509..2886)
                     /locus_tag="VE486_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZA38602.1"
                     /translation="MVLLDSDILIDYLRGLSAARNYFKSVDAAAISVVSVTELVSGAR
                     NDEEEAAMNELFSTMHIVGVDEKIAREAGRLRRQFLRSHNVETADALIAATSILHRLQ
                     MVTLNRRHYPMLADVLVPYRKRE"
     gene            complement(2886..>3110)
                     /locus_tag="VE486_05645"
     CDS             complement(2886..>3110)
                     /locus_tag="VE486_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZA38603.1"
                     /translation="TQIYLTEAEQRALRSLASKRKKAQSELIRQAVDEFISRSQRGHR
                     QALRERVFGIWKNRADVPDVRAMRRAWRGR"
BASE COUNT          741 a          867 c          849 g          653 t
ORIGIN      
        1 aatcaatccg agcagaacgg caacgtgcgc aagcgcaatc agggtaacgt ttcgccaaaa
       61 gtgcccactt gccacgccgt agctctgcga aggcgggtcc ttcggcatct atttcgtctc
      121 ggcttttgtg gcgatgccga ccttgctgat tcccgctcgt tgcaccgcat ccatcagtcc
      181 gattaatttt tgtacaggca gatcgcgatc gggtcgaatc acgaccgcca catccggatt
      241 ggctttcttc aactgaacga gttgcgtggt gagtgtctcc aagtccacca cttgattgtt
      301 aaatcggagc gtctcgtcgc ggtcgatcga cactgtttgg acctgtgacg agttgatcgg
      361 ttgattagtg gctccgctgg atgggatcga caggttcatg ctgttttcga gcagcggcgt
      421 cgtgatcatg aaaataatca gcagcacgaa agcgagatcg agcagcggcg tgatattaat
      481 ctcgctcagc gtggtcaggc cggtgcgttg tgaatagcgt ctcatttgtt cctgcttcga
      541 tgttgggcgt tggatgttgg atgttcggcg tcctctttcg atcggaaaca tcgaacaccg
      601 aacatcgaac tctcaacatc gaaccagttc atcgatctca tttgcggaag gccgattccc
      661 gcgagctgtg atcgacgtat ttgtgctcaa actcggaggc gagctcggcg gcaaagttat
      721 ccatttccac gataatgccg cgaatgctgg tgacgagaaa gttataaccg aacatcgccg
      781 gaatcgcgac acagagcgca gtcacagtcg tgatgagcgc gccggaaaca ccaggcgcca
      841 tcgacaccag gctaggcgaa cctgcttcgg ctacgcccgt aaaggcgtcc atcacgcccc
      901 aaaccgtgcc gagcaatccg agaaaaggcg aaccgctcac cgcggtggcc agcaaaatca
      961 tttgcgattc gagcgccagc gccgtttcgc ccaccgctcg ttccatcgcc gcactgaccg
     1021 cgcccatttg cgccggcgaa atcttatcgg cgatcccgag tcgcgcgcga aaggtgtcgt
     1081 ccacttccgg cgaaccgaga agatgaaatg tcatttcctc gcagccggcg cgatagacat
     1141 taaataccgg cgaccccggg aaccgtgcgt tcttttcaaa caagcgcagc ggttgccggt
     1201 cttgccgaaa agccgcgagg aaccgggcgt tttgctgccg cgcgaagcgg ataacgcgca
     1261 gcttcgtgac catgatcgac caactgaaga ttgaagcgac ggcgagaaga gagaggacga
     1321 gctttcctgc gatcgtcgcg tgatcgaacg caaaaaggag accgccggcc gctagcaatg
     1381 tcgcaatgag catctccgcg tcaccggcat aagtaatagc gccgttttgc agaaacgaaa
     1441 gcaagattcc aatgacgaag cacggatgac gaatgacgaa ggaagcccga atgctcaaat
     1501 gacgaaagct gccgtgaatg ctttgtcatt cggatttcgt cattccttcg tcattagaca
     1561 ttagtcgttc gtcattttct tgggaaatgt cggtctcatc ctggccgcga caacaaacgt
     1621 gatgccgatc gcaatcagga aaaacgaaaa gaactggccg cgcgtgaaag cacctatcat
     1681 cgccgcatcg ggctcgcgga agaattccgt cgcgatccga aaaatcgcgt agaagataaa
     1741 gaatagtccg gtcaatactc cgttgggttg gcgtgtccgc gttcgcacca gccacaggat
     1801 tgcgaacaaa acgatccctt cgaaaaaggc ttcgtagagt tgcgatgggt gccgcggagt
     1861 caggatcgag cgcagcgcgg aggcgacctg tggttgatga tttaccgcgg cgataatggc
     1921 ctctggcgtg gtgagcgctg gatcaatttg cgcgcatgtg cggattgcgc gttccgcttc
     1981 ggcgggatga tcgagcagct cttttggaaa ctgcattgcc catggaacat ttgtgatgcg
     2041 cccatacagc tcgccgttga tgaaattggc gcaacgacca aagaacaaac caagcggcgc
     2101 ggttacgacc aggttatcgc ccaggttcag ccacgagatt ttgtgccgat gggcatagta
     2161 aaaggtgaag agcaacaccc caagcatccc gccgtggctc gacattccgc cttcccagac
     2221 gcgcacgatc gacaatggct cgcgcaacat ttccggtttg tagaagaaaa cgtaccccag
     2281 ccggccaccg atgattacac cgaaaagcgc gcagcccgtg ataaagtctc caaccttttc
     2341 cacaggcaga tcggcataac cggttttcgc caggacgcgt agaaggaggt agctgcaaat
     2401 gaacgccagc acatatgcca gcccgtacca gcgcggcccg acattgtcgt aaatccgaaa
     2461 aataatcgga tcgagattgt ggagataata agcgagcatc aggaattgct actcgcgttt
     2521 gcggtaggga accagaacat cagcgagcat cggataatgc ctgcgattca aggtcaccat
     2581 ttgtaagcga tgcaaaatgc tggttgccgc gatgagagca tctgccgttt ctacgttgtg
     2641 gctgcgcaga aactgacgcc gcagcctgcc cgcttcgcgc gcgatttttt catctacgcc
     2701 aacaatgtgc atcgtgctga agagttcgtt cattgccgct tcctcttcgt cattgcgcgc
     2761 gccggagaca agttccgtaa cactcacgac cgaaatagcc gctgcatcca ccgatttgaa
     2821 ataattcctg gcagcggaca agccgcgcag ataatcaatc aatatatcgc tgtccagcag
     2881 caccatcacc gtccgcgcca ggcgcgtcgc atcgcacgca catcgggaac atcagcgcga
     2941 tttttccaaa tgccgaacac gcgttcacgg agtgcctgcc gatggccgcg ctgcgagcgg
     3001 gaaatgaatt catccaccgc ctgccggatt aactcgctct gtgctttttt tctcttggaa
     3061 gcaagcgagc gcagcgcgcg ctgttccgct tcagtcaaat aaatctgcgt
//
LOCUS       DASQUQ010000197         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_223724, whole genome shotgun sequence.
ACCESSION   DASQUQ010000197 DASQUQ010000000
VERSION     DASQUQ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223724"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VE486_05650"
     CDS             complement(<1..261)
                     /locus_tag="VE486_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZA38604.1"
                     /translation="MTAENRKQRLRRLDLLFVHSPTYFVTACTEHRRKLLATAAVHET
                     FLRFGQEGPAHGAWIGAYVIMPDHLHLFIATDNEKVTLAAWMK"
     gene            complement(271..1341)
                     /gene="hemA"
                     /locus_tag="VE486_05655"
     CDS             complement(271..1341)
                     /gene="hemA"
                     /locus_tag="VE486_05655"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZA38605.1"
                     /translation="MTLFCVGLSHHTANVETRERFAGHAETQCILRNAGCAEALLLTT
                     CNRVEVYGASEKRVSTDEIARCLAREIEKDAHEYAPAFYRYEGEKCVQHLFRVASGLD
                     SMVVGESEILGQAKKAYEAARSSRTAGPYLHRLFQRAFRVAKSVRTHTDITRGLVSVG
                     SVAVDLAQRIFGRLSNCKVLVLGAGETSERTARALASRGVNDLRVSNRSSDRARELAR
                     IVGGQAIPFEQWPEQSREIDILITSTSSETPLLTPENLAPMLRDRIDRPLFIIDIAVP
                     RDVDPRVNEMEGVYLYDIDSLQSVAEQSLALRRQQIAAAEAIIAEHVADFGDSISRGL
                     NCVARTAEPPPVADGSLRASQL"
     gene            complement(1338..2132)
                     /gene="ccsA"
                     /locus_tag="VE486_05660"
     CDS             complement(1338..2132)
                     /gene="ccsA"
                     /locus_tag="VE486_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZA38606.1"
                     /translation="MDRHLLIVSTICFLAAVVRTGVALRAGIFRPGQFNFLAIGLGFI
                     FQTAFLSIRGHELGRCPLTNLFEVFIFLAWSVALIYMLVGPAYRLSLMGAFTAPLVVL
                     IQGFALIAPIDVRHPVKLPANPWLEFHASISLVSYGAFALACIAGVMYLVQERQLKTH
                     QLHSIFYHLPPLTDLFAAITHLLWWGFVLYTLGLVSGFFTGGPLPRAQLVAAIGVWLL
                     YAAILQGRHLRRLAPKRVATLCILAFTAALTLLWGISFSAQNQSLP"
     gene            2225..2647
                     /locus_tag="VE486_05665"
     CDS             2225..2647
                     /locus_tag="VE486_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38607.1"
                     /translation="MNIKRLILAIVVAFIVLWVTDFLIHGVWMTPDYRATQQLWRTDS
                     EMMSRIGWMLGAQLLFVITFVIVWAKGFAERTSNISCALGYGLLMGLFSGVWALIMYV
                     VVPMPGSIAAKWFFAGIAQSILLGLITFWIYKPSAHAT"
BASE COUNT          685 a          782 c          799 g          587 t
ORIGIN      
        1 tttcatccag gcagctagtg tgactttttc attgtcagtc gcgatgaaca ggtgcaaatg
       61 atctggcata atcacataag cgccgatcca tgccccatgc gcagggcctt cttgaccaaa
      121 gcgcagaaaa gtctcgtgga cggctgcagt tgcgagaagt tttcgccgat gttcggtgca
      181 ggctgtgacg aagtaggtcg gcgagtgtac gaacagcagg tcaaggcgac gcaggcgctg
      241 ctttcgattt tcggcggtca tagaccgccg ctacaactgc gaagcgcgca gcgaaccatc
      301 cgcaaccgga ggaggttcgg cggttcgtgc aacgcaattc aatccacgcg aaatagaatc
      361 gccaaaatcc gcgacatgtt cggcgatgat tgcttccgct gcggctattt gctggcgtcg
      421 gagcgcaagc gattgctccg cgaccgattg caacgagtcg atatcataaa gataaacgcc
      481 ttccatttca tttaccctgg gatcgacatc acggggcacg gcgatgtcga tgatgaaaag
      541 cggacgatcg atgcggtcgc gtagcatcgg cgccagattc tccggcgtca gcaacggcgt
      601 ttcggaagat gtggaggtaa ttaagatgtc gatctcgcgc gactgttccg gccattgctc
      661 aaatggaata gcttggccgc cgacaattcg tgccaattcc cgggcgcgat cgctcgaacg
      721 attactcacg cgaagatcgt tgacgccacg tgaggcgagc gctcgtgctg tgcgttcgct
      781 cgtctcgccc gctccgagga caaggacttt gcaattgctg agtcgcccga aaattcgttg
      841 cgcgaggtcc actgcaaccg agccaaccga gaccaggccg cgcgtgatgt cggtgtgcgt
      901 gcgcacggac ttggccaccc tgaaggctcg ttgaaacaag cgatgtaaat atgggccagc
      961 cgtgcgcgat gagcgcgctg cttcgtaggc ctttttcgcc tgaccaagaa tctcactctc
     1021 gcccaccacc atcgaatcaa gacctgaggc gacgcgaaag aggtgctgca cgcatttttc
     1081 gccttcatag cggtagaatg caggcgcata ctcgtgggcg tccttctcga tctcccgcgc
     1141 aaggcagcgc gcgatttcat cggtcgagac ccgcttttcc gaagccccgt aaacttccac
     1201 tcggttgcag gtcgtaagca acaaagcttc cgcgcatccg gcattgcgca aaatgcattg
     1261 ggtctcggcg tgacctgcga agcgttcgcg cgtttccacg tttgcggtgt gatggctcaa
     1321 gccgacgcag aaaagagtca tggcaacgac tgattttggg ccgagaaact gattccccag
     1381 agaagcgtca gggccgccgt aaatgcgaga atgcaaagcg tggccacgcg tttgggcgca
     1441 aggcggcgaa gatggcggcc ttgcaagatg gcggcataga gcagccagac cccgatagcg
     1501 gccaccagtt gcgcccgcgg caacggtcct cccgtaaaaa acccgctcac gagcccaaga
     1561 gtataaagca cgaaacccca ccagaggagg tgtgtgattg ccgcgaagag atcagtcaag
     1621 gggggcaaat gataaaaaat ggaatgcaat tgatgcgtct tgagctggcg ctcctgcacg
     1681 aggtacatca cgcccgcaat gcaagcgagc gcgaacgcgc cataggacac gagcgaaatc
     1741 gaagcatgaa attccagcca cggatttgcc ggcagcttca ccggatgccg gacgtcgatt
     1801 ggcgcaatca aggcaaatcc ttgaatcaaa accaccagcg gcgccgtgaa cgcgcccatg
     1861 agcgaaaggc gataggccgg gccgaccagc atgtagatca acgccaccga ccaggcgaga
     1921 aagataaaca cctcaaataa gttcgtgagc ggacagcgcc ctaactcatg accgcggatc
     1981 gataaaaacg ctgtctgaaa aataaatccg agcccgatcg ccagaaaatt aaattgtccc
     2041 ggtcgaaaaa tgccagcacg caaagcgact ccagtccgca cgactgccgc gagaaaacaa
     2101 attgtcgaaa cgatgagaag atgccgatcc actctgaaaa agaagtgtaa aagctgcaca
     2161 ggagcttggc aaaccacgct ttccttgcac gctcgcgcga aaatccctac aactgccggt
     2221 taatatgaac atcaaacgtc ttattctcgc aattgtcgtc gcgttcatcg ttctttgggt
     2281 aactgatttt cttattcacg gggtctggat gacacccgat taccgcgcca cacaacagct
     2341 ctggcgtact gattccgaaa tgatgtcacg catcggttgg atgctcggcg ctcagctctt
     2401 gttcgtgatt accttcgtga tcgtttgggc aaaaggtttc gccgaacgca cgagcaatat
     2461 cagttgcgct ctcggctacg gtttgctgat gggattgttc tcgggcgtct gggcgctcat
     2521 catgtatgtt gtcgtgccaa tgcctggctc aattgctgcg aagtggtttt tcgccggcat
     2581 tgcgcaaagc attctccttg gcctcatcac gttctggatt tacaagccgt cggcgcacgc
     2641 gacgtaattt gtgcggtcaa cgttaaggag gatctcgcag cacaatcgcg gccttttgga
     2701 taagatcgcg ccgctggatt gaaccggcgt tgacgttccg gagcaagccg ctagcgtcgc
     2761 atcgatgaat tcagaagtaa tggataagct gcgcaccggt gtgcgtgatg tgcctgattt
     2821 tcctaaaaaa ggaatcgtgt tcaaagacat cac
//
LOCUS       DASQUQ010000198         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_705859, whole genome shotgun sequence.
ACCESSION   DASQUQ010000198 DASQUQ010000000
VERSION     DASQUQ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705859"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2593)
                     /gene="carB"
                     /locus_tag="VE486_05670"
     CDS             complement(<1..2593)
                     /gene="carB"
                     /locus_tag="VE486_05670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZA38608.1"
                     /translation="MPRNNQLNKILLIGSGPIVIGQGCEFDYSGVQACKALREEGYQV
                     VLVNSNPATIMTDPEFADRTYIEPITPEVIEAILEREKPDAILPTLGGQTALNAAMEL
                     NRNGALARHGVKLIGANAQAIAKGEDRQLFKEAMLRIGLEVPRSGIARSLADVDRVVE
                     EIGTFPLIIRPAFTLGGTGGGIAYNREELDVIVVRGLDLSPVREVLIEESLLGWKEFE
                     MEVMRDRMDNCVVVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQMMRDAAFAVIREI
                     GVETGGSNIQFAVNPDNGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLD
                     EIKNDITRETPACFEPTIDYCVVKVPRFTFEKFPQADATLTTRMKSVGEAMAIGRTFK
                     EALQKALRSLEIKRFGLCSDGAERFVNPETLRLKLSIPNADRIFYLAQAFQDGLSIDD
                     VFELTKIDRWFLRNVQQIVAEADRRLGFQPDSAGGLPACQNPSARQARSLPSASGKMA
                     DRRFRPFDEQRAVKITKRHLPHWEQPGATYFVTFRLADAIASDVLAEWREECALWLKH
                     HPQPWDWKTAREYMRKFEEEREQWLDQGHGSCLLRDSRSQEIIANALSHFDRDRYIID
                     AFVVMPNHVHILLKPLGANSLGVILHSWKSFTSNEINRLTKRSGPLWMTETFDTIVRD
                     SEHLTFCRNYIEQNPEKAHMSRDECLLDRRDVLLPEDSHAEAGQARSLPAESGWKPNL
                     RRESLLRAKKLGFSDRQLAVAEATGENEIRQRRISAGVIPTYRLVDTCAAEFEAYTPY
                     YYSTYGDENERRESGKRKIMILGGGPNRIGQGIEFDYCCVHAAFALRELGFETIMVNS
                     NPETVSTD"
     gene            complement(2777..>3172)
                     /locus_tag="VE486_05675"
     CDS             complement(2777..>3172)
                     /locus_tag="VE486_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HZA38609.1"
                     /translation="ERRETLVSYADNAKRQEPREGENFDLVDVRKDEDCTDTVGVYLH
                     VDEQGKAQIRMTLGPEVQTLTRSMTAILCKGLEGSTPQEILDLPSDFVTRIVGAELVR
                     VRSQTIYYVLTRIKSICKVYLDRQRMAVA"
BASE COUNT          636 a          926 c          891 g          719 t
ORIGIN      
        1 aatcggtgga aacggtttcc ggattcgaat tgaccatgat ggtctcaaat ccgagctcgc
       61 gcagggcgaa ggcggcatgg acgcagcagt aatcaaactc gattccctga ccgatccggt
      121 tcggcccgcc accgaggatc atgatcttgc gcttgccgct ctcgcgtcgc tcgttctcat
      181 cgccgtaggt ggaatagtag taaggcgtgt aagcttcgaa ttctgccgcg caggtatcca
      241 cgagccggta ggtcggaatt acgccggcgg atattcgccg ctggcgaatt tcgttttcgc
      301 cggtcgcttc ggcgaccgcc agttgtcgat ccgaaaaacc gagcttcttg gcacggagga
      361 gtgactctcg ccggaggtta ggcttccagc ctgactcggc aggcaagctt ctagcttgcc
      421 cggcttccgc gtgagaatcc tccggcagga gaacatctcg ccgatccagc aaacattcgt
      481 cgcggctcat gtgcgccttc tccggatttt gctcaatgta attgcggcaa aaagtcagat
      541 gctcggaatc gcgtacaatc gtatcgaaag tttccgtcat ccaaaggggt ccgcttcgct
      601 tcgtcaggcg attaatctcg ttgcttgtga aggacttcca cgaatgcaag atcacgccaa
      661 gcgaattcgc gcccaacggc ttcaggagga tgtggacgtg attcggcatc accacgaagg
      721 catcgatgat gtaacgatcg cgatcgaaat gactaagggc attggcgata atctcctgcg
      781 accgggaatc tcttagcaag caggagccgt gaccttgatc caaccattgt tcgcgttctt
      841 cttcgaattt ccgcatgtat tcgcgcgcgg ttttccaatc ccacggttgt ggatgatgct
      901 ttagccatag tgcgcattcc tcgcgccact cggccagcac gtcgcttgcg attgcatcag
      961 cgaggcgaaa cgtaacgaaa taagtggcgc cgggttgctc ccagtgaggc aaatgacgtt
     1021 ttgtaatttt gacggcccgc tgttcgtcaa agggccgaaa tcggcggtca gccatcttgc
     1081 ctgacgcgga gggcaagctt ctagcttgtc tagctgacgg gttttggcag gctggaagcc
     1141 cgcccgccga gtcaggctgg aagcctaacc tccgatccgc ttctgccacg atttgctgca
     1201 cgtttctcag aaaccatctg tcgatctttg ttagctcgaa gacgtcgtcg atcgacagcc
     1261 catcctggaa tgcctgggcg agataaaaaa tccgatccgc gttggggatt gagagcttca
     1321 atcgcagcgt ttccgggtta acaaatcgct ctgcgccatc gctgcataat ccgaaacgtt
     1381 tgatctccag actgcgcagc gccttttgca atgcttcctt gaacgtgcga ccaatcgcca
     1441 tcgcttcgcc gacgcttttc atccgcgtcg tcaaggttgc gtcggcctgc ggaaatttct
     1501 cgaacgtgaa ccgcggcact ttgacgacgc agtaatcgat ggttggctcg aagcacgccg
     1561 gcgtttcgcg cgtgatatcg tttttgatct catcaagggt gtaaccgacg gcgagttttg
     1621 ccgcgatttt ggcaatcgga aagccggtcg ctttcgatgc cagcgcgctc gaacgcgaga
     1681 cgcgcggatt catttcaatc acaaccatcc ggccattgtc cggattgacc gcgaactgaa
     1741 tgtttgagcc gccggtctcg acaccgatct cgcgaatgac cgcgaacgcg gcatcgcgca
     1801 tcatctggta ttccttgtcg gtgagggttt gcgcgggcgc gaccgtaatc gagtccccag
     1861 tgtgtacgcc catcggatcg aaattttcga tggaacaaac gaccacacag ttgtccatgc
     1921 gatcgcgcat cacttccatc tcgaactctt tccaacccag gagcgattct tcgatcaaca
     1981 cttcgcggac gggtgagaga tcgaggccgc gcacgacgat gacgtcgagt tcttcgcggt
     2041 tgtaggcgat gccgccgccg gttccgccga gagtgaatgc cggccgaata atgagcggaa
     2101 atgttccgat ttcctcgaca acgcgatcga cgtcggccag cgagcgcgcg atgccagacc
     2161 gaggcacctc cagaccgatg cgcaacatcg cttccttgaa caattgccga tcttcgcctt
     2221 tcgcgatggc ctgtgcgttt gcgccgatca atttcacgcc gtgccgtgcg agcgcgccgt
     2281 tgcggttcaa ttccatcgcg gcattgagcg cggtttgtcc gcccagggtc ggcaaaatcg
     2341 catccggttt ttctcgttcc aggatcgctt cgatcacttc gggggtgatc ggctcgatgt
     2401 aagtgcgatc ggcaaattcc ggatcggtca tgatggtggc cggattcgaa ttcaccagca
     2461 cgacctggta accttcttcg cgcagcgcct tgcaggcttg cacgccggaa taatcaaatt
     2521 cacagccctg cccgatcacg attggaccgg agccgatcag caggatttta ttgagctggt
     2581 tatttcgcgg catctgcgcg cgggatgata aatcaaatca gccgaagttc acgcgcgaat
     2641 cgtggcacag gcatcttgtc cgccacggga cggactcgct gcggcgagcc tgtggggcaa
     2701 gcgggtcccg cctgctgggt taccactcga ctcgacaggc gaggcgcccg tcggccccac
     2761 agccgggacg gccgtgctac gcaaccgcca tgcgctggcg atcgagatac actttgcaga
     2821 tgcttttgat acgcgtgagc acgtaataaa tcgtctggct acggacgcgc accagttccg
     2881 cgcccacgat tcgcgttacg aaatcgctcg gcagatcgag aatctcttgc ggcgtcgagc
     2941 cctcgagacc tttgcaaaga attgccgtca tcgaacgggt gagtgtttgc acttccgggc
     3001 caagcgtcat gcggatctgc gccttgccct gctcatcgac atgcagataa acgccgaccg
     3061 tgtccgtgca atcttcatcc ttgcggacat caacgagatc gaaattctcg ccttcgcgcg
     3121 gctcctgtct cttcgcgttg tcagcatagg agacgagtgt ctcgcgccgc tc
//
LOCUS       DASQUQ010000199         6022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_409332, whole genome shotgun sequence.
ACCESSION   DASQUQ010000199 DASQUQ010000000
VERSION     DASQUQ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409332"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VE486_05680"
     CDS             <1..837
                     /locus_tag="VE486_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HZA38610.1"
                     /translation="VTLTLGAKGVIEVELTATGEKWGRGPGKDIHSSLKAMVDSPAWH
                     LVKALNTLTSEDGNETTIDNYPKPRPLSVQEKKMIADAAKRRSEAAEKKRLSVKHWIN
                     DLPWQQALERLQSQPTVNIEGLVGGYTGPGGKTILPHKAVAKLDLRLVPNMTAADALA
                     ALKAHLAKRGFGDIEVNMTGGYDPNSTSADSGLIKSQIAVFKNSGIDPIILPRSAGSW
                     PGYVFTGAPLNKPAGHFGLGHGSGAHAPDEYFVIESSNPKIQGMDGATMSYIEYLYEL
                     AK"
     gene            complement(856..1380)
                     /locus_tag="VE486_05685"
     CDS             complement(856..1380)
                     /locus_tag="VE486_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38611.1"
                     /translation="MMNWALFALSITGVMTAIWIVVLVKSRRSFMSIYVSFAFLLLAA
                     MNSAAPIRGYVDPNYVGYGFGLLHATKGLSVTLMAGGVFLISAVSAFFAARNRRGPGM
                     WIVVVACLAFAVIEGWPWLEGVLHDPAGNAIQFGEYLTIPGLAASALLGALLVVPFVL
                     GAPWAARRALARGC"
     gene            complement(1383..2948)
                     /locus_tag="VE486_05690"
     CDS             complement(1383..2948)
                     /locus_tag="VE486_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZA38612.1"
                     /translation="MNSPEIGARGTPGSSRFAAEQKLIVFFAAVSLLIHFLTNGRYGY
                     FRDELYYIACGQHLAFGYVDLPPLSALLIRLSRVLLGDSLFAIRFLPAVASALSVALT
                     GMIARELGGRIWAVALSCTASLCALVFLAVGNFFSMNAFEPLFWMGAIYLLVRIINGG
                     SPQLWLWFGGVVGLGLENKHSMAFFAAGVCAALLLTPERRHFAQKWIWLGGLIAVALA
                     LPNVIWQIRHQWPTWELLHNIAQSNKNVVLTPAQFMAQQVLIMNPATLPLWLGGLIWL
                     LASREGRRYRVIAIVYLITLAEFIVMHGKHYYLAPAYPMLFAAGGVGGERLFSIRLQW
                     LKPALLAAMIALAALLAPTVVPILSPEKLVAYMKSIHFKLPRTETSHTAALPQLFADQ
                     FGWEEMVRSVARAYATLAPEDQKRVAIFCQNYGQAGAIDFFGPKLGLPPAISGHQNYF
                     LWGPHDYTGEVLLVLDYDADDERQQFESVEDLGQVLSSPWAMPWEQRSHIYLCRRLKG
                     TLDDLWPKLKEWM"
     gene            3032..4057
                     /locus_tag="VE486_05695"
     CDS             3032..4057
                     /locus_tag="VE486_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38613.1"
                     /translation="MRRVEAADWTSLSDEELVERRISTLSLRLEGTAPEPLIRRLYDE
                     LSAKGLTFHPPTHIGDEWFVPIGIPAIFIPFFLVHDRLRALERTMMLEVEGETPEWFM
                     KLIRHEAAHAYSYAFQLQRKKKWQQTFGQTSDEETPSFYRPRPFSRSYVVHLDDWYAQ
                     AHPDEDFAETFAIWLTPGLDWRKRYERWKALRKLEYVDELMRSLAGQPPVHAPKYRAA
                     DYDCLNVKLKTYYARKRKTYEDLYPDFYDVDLKQLFPAPAGPGRIKASSYLRQRRRRL
                     MNSVCQWTNERKYRVNKLLTRLIERCDQLGLHVHNDDPQINFRVSAFITTLVMNYLFT
                     GKFKRTK"
     gene            4080..5099
                     /locus_tag="VE486_05700"
     CDS             4080..5099
                     /locus_tag="VE486_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZA38614.1"
                     /translation="MKKKLKVLVLFDGTSPTTVDQDFSKELKTKDWKTEADVMAALGA
                     LGYSAEHLAIFDDVDLLRQKLETFAPDVLFNLVEQFRNNPGFDQNIVSFFEMQGLPFT
                     GCGSTGLTLCKDKGISKKVLHYHGIHVPNFVVVPRGQRIGRPRQLKFPILVKPVKEEA
                     SYGISRASFVETDEQFRERVAFIHEKYNSDAIAEEYIEGRELYVSIMGNVKLTVFPIR
                     ELVFREVPLNEPKIATYSAKWDEEYRKRWGLENQFPDGFDPALVNHIEQTCKRIYRLL
                     TIDGYARIDLRLTAANEVYFIEANPNPILANDEDFALSANKAGLSYPQLIDRIVRQGM
                     RTIRT"
     gene            complement(5161..>6022)
                     /locus_tag="VE486_05705"
     CDS             complement(5161..>6022)
                     /locus_tag="VE486_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZA38615.1"
                     /translation="TAITPGYFDAIGVKLLRGRDFTQAEAEKKDTPRVAIIDDEMAKK
                     IFPKTEAVGQHIRYTQPPKDGSPNDLLVVGVVGSHRHDVQNDTLNRRLFVPLAQGYNG
                     NVYLHVRLATKDRHAVAAFIPTMRQTLRSVDPDLPILSIAPFVDLTEKSVGLWIVRLG
                     ALLFGVFGGIALLLAVVGVYGVKAYAVACRTREIGIRMALGARRRDVFALIMRQGALQ
                     TALAVAIGLFLSLAAGRVLSRILYEVSPSDPFALVASGALLAAAALLACFFPARRATH
                     VEPLQALRTE"
BASE COUNT         1450 a         1679 c         1773 g         1120 t
ORIGIN      
        1 gtgacgctga cgcttggcgc gaaaggcgtg atcgaagttg agctgacggc gaccggcgaa
       61 aagtggggac gcggcccggg gaaagacatt cactcatccc tgaaagcgat ggtagacagc
      121 ccggcctggc atttggtcaa agcattgaat acgctgactt cagaggacgg caacgagacc
      181 acgatcgaca actatccaaa gccgcgtccg ctcagcgttc aggagaaaaa gatgattgca
      241 gatgcagcta aacgtcgcag cgaagcggca gagaagaagc gattgagcgt gaagcattgg
      301 atcaatgatc tgccatggca gcaggcgctc gaacgattgc agtcacagcc aacggtaaac
      361 atcgagggtt tggtcggcgg ttataccggt cccggcggca aaacgatttt gccgcacaag
      421 gcggtggcga aattggatct gcgtctcgtg ccgaacatga cggcggcgga cgcgcttgca
      481 gcgttgaagg cacatctggc caagcgcggc ttcggcgata tcgaagtgaa catgaccggc
      541 ggttacgatc cgaacagcac ctcggcggac agcggcctga tcaaatcgca aatcgccgtt
      601 ttcaaaaatt ccgggatcga tccaatcatc ttgccgcgaa gcgccggctc atggccgggt
      661 tatgttttta ccggcgcacc gctgaacaag ccggccggac actttggcct cggccacggc
      721 agcggggccc atgcgcctga tgaatatttc gtgatcgaat cgtcgaatcc gaagattcaa
      781 ggaatggacg gcgcgacaat gtcgtacatc gagtatttgt acgagctggc aaagtgagca
      841 gttcgcgtgc gagaactagc atccccgcgc gagggcgcgg cgcgcggccc aaggtgcccc
      901 gagaacgaag ggcacaacca gaagcgcgcc gagcagcgca ctggctgcca agccgggaat
      961 ggtgagatac tcgccgaact gaatcgcgtt cccagcgggg tcgtggagca cgccttccag
     1021 ccagggccat ccttcaatta ccgcgaaggc caaacaagcg acgaccacga tccacatccc
     1081 gggaccacgc cggtttcgcg cagcgaagaa cgcagagact gccgaaatca aaaagacgcc
     1141 gcctgccatc aatgtcacgg acaacccttt ggtggcgtgc agcaggccaa agccgtagcc
     1201 gacatagttt ggatcgacat atccgcgaat cggcgcggct gagttcatgg ccgcgagcag
     1261 caaaaaggcg aaggaaacgt agatcgacat gaacgagcgc cgacttttga ctagcacaac
     1321 gatccatatc gcggtcatga cccccgtgat cgagagagcg aagagagccc aattcatcat
     1381 tttcacatcc actcttttag ttttggccaa aggtcgtcaa gcgtcccttt aagacgccgg
     1441 cagaggtaaa tgtgactccg ttgctcccac ggcatcgccc aggggctgct caggacctgg
     1501 ccaaggtctt cgaccgattc gaactgctgc cgttcgtcat cggcgtcgta atcgaggacg
     1561 aggagaactt cgccggtgta atcgtgcgga ccccagagaa aataattttg atgccccgag
     1621 atcgcgggcg gcagtccgag cttgggtccg aaaaagtcga tcgcgccggc ttggccgtaa
     1681 ttttgacaga aaatcgcgac ccgcttctga tcctcgggcg ccagagtcgc ataggccctt
     1741 gcgactgagc gcaccatctc ttcccagccg aattgatcgg cgaaaagctg tggcaacgcc
     1801 gcagtgtgcg aagtctcggt gcgcggtagc ttgaaatgga tcgatttcat gtaggcgacc
     1861 aatttttccg gggagagaat cggcacgaca gtcggcgcaa gcagtgcggc aagtgcgatc
     1921 attgcggcaa gcaatgcggg cttgagccat tgcaatcgaa tgctgaagag acgttccccg
     1981 ccaacgccgc cggcagcgaa gagcatcgga taggcgggcg cgagatagta atgttttccg
     2041 tgcatgacaa tgaactcggc gagcgtgatc agataaacaa tagcgatgac gcggtagcgg
     2101 cggccctcgc gggaggcgag tagccaaatg agcccgccca gccacaacgg aagcgtagcc
     2161 ggattcatga tgaggacttg ttgcgccatg aattgcgcgg gcgtgagcac gacgtttttg
     2221 ttgctctgcg caatgttgtg caacagctcc cacgtgggcc attgatgccg gatctgccaa
     2281 atgacgtttg gcagcgccag cgcaactgcg atcagtccgc cgagccaaat ccatttctga
     2341 gcgaaatgac ggcgctcggg cgtaagcaac agcgcagcgc agaccccggc ggcgaagaaa
     2401 gccatggaat gtttgttctc cagcccgaga ccaacgaccc cgccgaacca caaccaaagc
     2461 tgtggcgaac cgccattgat aatccggacg agcagataaa tggcgcccat ccaaaacagc
     2521 ggctcaaacg cgttcatgga aaagaaattg ccgacggcca gaaagacgag cgcgcaaagc
     2581 gaagcggtgc agctgagcgc gactgcccaa attcgtccgc cgagctcgcg cgcgatcatt
     2641 ccggtcaagg caacgctgag tgcgctcgca actgctggaa ggaaacggat tgcgaaaagt
     2701 gaatcgccga gcaggacgcg gctcagtcga ataagcagcg cggaaagcgg cggtagatcg
     2761 acataaccga aggcgaggtg ttgaccgcag gcgatgtaat agagttcgtc gcgaaaataa
     2821 ccgtaacggc cgttggttag aaaatggatg agcagactca ccgcagcgaa gaagacgatc
     2881 aatttttgtt cggctgcgaa ccgacttgat cctggcgtcc ctcgcgcgcc gatttcgggc
     2941 gaattcatgg gaagcgatta cgcgcaaatc gtcgctgaaa ttcaagccgc ttcgtgcgaa
     3001 agcgtgacag cgctcggcgc ggactgatat tgtgcgcaga gtggaagctg cggattggac
     3061 gtcactgagc gacgaggaac ttgtcgagcg ccgcatttcc acgctcagtt tgcggctcga
     3121 gggcacggcg ccggaaccac taatccggcg gctttacgac gagctgtcgg caaagggact
     3181 cacgtttcat ccgcctactc acatcggcga tgaatggttc gtgccgatcg gtatcccggc
     3241 catctttatt ccgttttttc ttgtgcacga tcggctgcgg gcgctggagc gcaccatgat
     3301 gctcgaagtc gagggcgaga cgccggagtg gttcatgaaa ttgatccgtc acgaggcggc
     3361 gcacgcttat agctacgcgt ttcagcttca acgaaagaaa aaatggcagc agacttttgg
     3421 acaaacgtca gacgaggaga ccccgagctt ttatcggccg cgcccgttca gccgcagtta
     3481 cgtcgtgcat ctcgacgatt ggtacgcgca ggcccatccg gacgaggatt tcgcggagac
     3541 gttcgcgatc tggctcacgc cggggctcga ctggcgcaag cgttacgaga gatggaaggc
     3601 tttgcggaaa ctcgaatacg tcgatgaatt gatgcggtcg ctggcggggc agccgccggt
     3661 gcacgcgcca aaatatcgcg ccgctgatta cgactgcctg aacgtcaagc tcaagaccta
     3721 ttacgcacgg aagcggaaga cctacgaaga tttgtatccg gacttctacg atgtcgatct
     3781 gaagcagctt ttccctgcgc cggccgggcc gggaagaatc aaagcgagct cgtatctgcg
     3841 gcaacggcgc cggcggctga tgaactcggt atgtcagtgg acgaacgaaa gaaaatatcg
     3901 cgtcaacaaa ttactgaccc gattgatcga gcgttgcgat cagctcgggt tgcacgtgca
     3961 caatgacgac ccgcagataa attttcgcgt ctccgcgttt atcacgaccc tggtcatgaa
     4021 ttatctgttc accggtaagt tcaagcgcac caaataagaa cagaaacaaa cagttgtttg
     4081 tgaaaaagaa actcaaagtc ctcgttcttt tcgacggcac aagtcccacc accgtcgatc
     4141 aggatttcag caaggagtta aaaacaaagg attggaaaac cgaggcggac gttatggccg
     4201 cgcttggcgc gctcggttat tcggcggaac atctcgcgat tttcgacgat gtcgatcttc
     4261 tccggcaaaa actggagacg ttcgcgcccg atgtcctttt caaccttgtc gaacagttca
     4321 ggaacaaccc cggcttcgac cagaacatcg tttcgttttt cgaaatgcaa ggccttccgt
     4381 ttaccggttg cggctcgacg ggtcttaccc tttgcaagga caagggcatt tcgaaaaagg
     4441 tcttgcatta tcacggtatc cacgtgccga acttcgtcgt cgtcccgcgc ggccagcgca
     4501 tcgggcgacc caggcagctc aagtttccga tcctggtaaa gccggtgaaa gaagaagcct
     4561 cttacggaat ttcgcgggcg tcgttcgtcg agaccgatga acagttccgg gagcgggtcg
     4621 cttttattca cgagaaatac aacagcgacg ccatcgccga ggaatacatc gagggccgcg
     4681 aactctacgt cagcatcatg ggcaacgtga agctgaccgt ctttccgatc cgtgagctgg
     4741 tcttccgcga agttccactg aacgagccga agattgcgac ctatagcgca aagtgggacg
     4801 aagaatatcg caaacgctgg gggctggaga atcagttccc cgacgggttc gatccggcgc
     4861 tcgtcaacca catcgaacag acatgcaaac gcatctaccg tctgctcaca atcgacggtt
     4921 acgcgcgcat cgacctgcgg ctgacagcag ccaacgaagt ctatttcatt gaggcgaatc
     4981 caaatcccat tctcgccaac gacgaggact tcgcgctttc agcgaacaag gccggcctct
     5041 cttatccgca attgatcgac cggatcgtcc ggcaagggat gaggacgatt cggacgtgag
     5101 tgagggagac ccccgatctt caacattcaa cgcttaagtc tgaccacggc cacccgcgcg
     5161 tcattcggtg cgcaacgctt gcagcggctc aacatgcgtg gcgcggcgcg ccgggaaaaa
     5221 acaagcaagc aaagcggcgg cagcgagcag ggcgccggat gcgaccagcg cgaatggatc
     5281 gctcgggctg acttcataaa gaatccggct gaggacccgg cccgcggcca gggaaagaaa
     5341 aagaccgata gccacagcca gcgcggtttg cagcgccccc tggcgcatga tcaatgcgaa
     5401 aacatcgcga cgacgcgcgc cgagcgccat gcgaatgccg atctcgcgtg tgcgacaggc
     5461 gaccgcgtat gcctttacgc cgtagacgcc aacgaccgcg aggagcaacg cgattccgcc
     5521 aaagactccg aagagcagcg cgccgagacg cacgatccaa agaccgacgc tcttttcagt
     5581 aagatcgaca aatggcgcga tgctcaggat tggcagatcg ggatcaacgc tgcgcaatgt
     5641 ctggcgcatg gtgggaatga aggccgcgac agcatgtcga tctttcgtgg caaggcgcac
     5701 atgcagataa acgttgccgt tgtagccttg tgctagcggc acaaacaatc gtcggttaag
     5761 cgtgtcattt tgcacatcgt ggcggtgact gcccacgacg ccaacgacga gcaaatcgtt
     5821 aggcgaaccg tcttttggcg gttgcgtgta acgaatgtgc tggcccaccg cttccgtctt
     5881 tgggaaaatt ttcttggcca tttcatcatc aataatcgcg acgcgcggcg tatctttctt
     5941 ttcggcttcg gcctgggtga aatcgcggcc acgcaatagc tttaccccga tcgcatcgaa
     6001 gtaacccggc gtgatcgcag tg
//
LOCUS       DASQUQ010000200         3491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_142899, whole genome shotgun sequence.
ACCESSION   DASQUQ010000200 DASQUQ010000000
VERSION     DASQUQ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142899"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="VE486_05710"
     CDS             complement(<1..1750)
                     /locus_tag="VE486_05710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA38616.1"
                     /translation="MLPSPFHFPGLDFARVLSRKFESGRLLVIGADRGKLEEQFAETG
                     REALVLGSPGDLSAKLGQGDSAAHFEIAVWFYPLEKSEDDRVAADLSRCADDIVLVPG
                     RGADVSKRRLQLVECLRRFGFLPDYECDLSDLAPGAIRLRRRPSEPADTLIPAAETAL
                     ARLHAQLAAFQRVHQIQESELEAAHQHIAALEEKLLKLKEYRRELKSLKEQKRILRKS
                     PERRVGQLLLAPYRLPEKLVKTIWRKLHPRDTRSRRLAPPTDYQEWLRRHRASVSDLE
                     RMRDEAHGFAFQPLISVITPVYDTPVPRLEEAVESVLAQVYENWELLLVDDGSTAADL
                     LDALPDLAARDRRITLAKPGKHEGISAASNRGLALARGEWVTFVDHDDVLEPDALFQV
                     AKLLQTHRNAEMIYSDEDKLAEDGYDAPLFKPDWSPDFFLSCNYLGHLTVLRRNIVGK
                     IGGFRSEFDSAQDYDLFLRVAEQTSRIHHIPQVLYHWRRSADSSAISVRQKPGQLDAT
                     RRAIEDYLERRGEPARVAVDWRTHTFCIRRELSEAKSISIVISSRNDPESLERCIESL
                     ASKTSYPSYEIVIVEDD"
     gene            1830..>3491
                     /locus_tag="VE486_05715"
     CDS             1830..>3491
                     /locus_tag="VE486_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZA38617.1"
                     /translation="MLDFSVYLLYRAGSAIASALPLPVLFVFGELLGFCAWIVLGNYR
                     RLAQRNIAIAFGSEKSPRELRRLVRRHFQRLGANLLCSVKIAAMPLERVRAHVKIENA
                     DAAHRELRAGRPIVFVLSHLGNWELQAQMFPVMIGYVRNSTIFQPLGNRYIDKHVRTL
                     RGRAGVELFDRREGFQKAIQLLRGGGAIGILSDQHSGDLGLWVPFFNKLASTSPLPGL
                     LAKRTGAALLGTAIYTDGLARWRMVFTESIGALGDSIEALTAKANELIAAQIRRAPED
                     WFWVHNRWKIPEPSLLLARYKRGIYLPPDISGQDLQPFRILIRSSNWLGDAVMSVPAI
                     RAIKNGRLDAHVTIATPAKIASMWKLVSEVDAIIPLSGDSLLTTIRLLRRQPSFDAAI
                     LFPNSLRVVLETWLSGIPRRVGYRGHARSWLLNQIITEPRRPGPLKHQSARYLRIAEE
                     LGAEITATLRLPPFHQQSTINHQPWKLGLCPGAEYGPAKRWLPERFAEAAAKVAAQAP
                     VQWVLFGTPKDAEIGDQIAAGLGDSCVNRIGQTTLDQLILALRECR"
BASE COUNT          720 a         1038 c          965 g          768 t
ORIGIN      
        1 tgtcatcttc gacgatgacg atttcgtagc tcgggtaact cgtcttgctt gccaggcttt
       61 caatacaccg ctcgagtgac tccggatcgt tgcggctcga aatgacgatt gaaatgctct
      121 tcgcctcgga tagttcccgt cggatgcaaa aggtgtgagt gcgccaatcc accgcgaccc
      181 gagcaggctc gccgcgacgc tccaggtaat cttctatggc gcggcgggtc gcgtccagtt
      241 ggccgggttt ttgtcgaacg ctgatcgcgc tggagtctgc gcttcgtcgc caatgataca
      301 acacctgcgg aatatgatga atccggcttg tctgctccgc gacgcgaagg aaaagatcgt
      361 aatcctgcgc gctgtcgaat tctgaacgga atccaccgat ttttccaaca atgttgcgtc
      421 gcaacaccgt caaatgcccg aggtaattgc aggaaaggaa aaaatctggc gaccaatcag
      481 gcttgaacaa cggcgcatcg tatccgtctt ctgccaattt atcttcgtcg gaataaatca
      541 tttcggcatt gcgatgcgtc tgcaagagct tcgcaacttg aaaaagcgcg tccggctcaa
      601 gcacatcgtc atgatcgaca aaggtcaccc attcgccgcg agcaagcgcg aggccgcggt
      661 tggaggccgc ggaaatgcct tcgtgtttcc ccggttttgc aagagtgatc cgccggtcgc
      721 gcgcagccag atcaggcaaa gcatccagca aatcggcagc ggttgatcca tcgtcaacga
      781 gaaggagctc ccagttttcg tagacctgcg ctagcaccga ttcgacagct tcttccagcc
      841 gcggcaccgg cgtatcatag acaggcgtga tcacgctaat taacggttga aaagcgaacc
      901 cgtgcgcttc gtcacgcatt cgttccagat cgctcacgct tgcgcggtgc cgccggagcc
      961 actcctgata atcggtgggc ggggccaatc gcctggaacg tgtgtcacgc ggatgcagct
     1021 tcctccagat tgttttcaca agcttttccg gaagacgata gggcgcgagt aacagttggc
     1081 cgactctgcg ctcaggcgac ttgcgcagga ttcgcttctg ctctttcaag gacttcagct
     1141 cgcgccggta ttctttcagt ttgagcaatt tttcctcgag cgcagcgatg tgctgatgcg
     1201 cggcttctaa ctcggattcc tggatttgat ggacccgctg gaaagcggcc agctgcgcgt
     1261 ggagtcttgc caaagcagtc tccgctgctg ggatcagcgt atcggcaggt tcgctcggcc
     1321 ggcgccggag gcgtatcgct cctggagcga ggtcgctcag atcgcattcg taatcaggga
     1381 ggaatccgaa tctgcgaagg cactcgacaa gttggaggcg tcgcttgctg acgtccgcgc
     1441 ctctgccggg caccaacaca atgtcgtccg cgcaacgaga cagatccgct gccacgcggt
     1501 cgtcctcact tttttccagc ggataaaacc agactgcgat ttcaaaatgc gctgcgctgt
     1561 caccctgccc aagctttgcc gataaatcgc ccggagatcc gaggacgaga gcttctcttc
     1621 ccgtttcggc aaattgctcc tcaagtttcc cacggtctgc tccgatcacg aggagccggc
     1681 cagattcgaa cttgcgcgag agaacgcgcg cgaaatcaag accagggaaa tgaaaaggcg
     1741 atggcaacac cgcgcacatt atattggttg aagcaagccg caggcaaatt taccctgccg
     1801 caccgtcgcg cgataccgca cgatcctgca tgctcgattt tagcgtttat ctcctttatc
     1861 gcgcaggatc ggcgattgcc agcgcgctgc cgttgccggt tctctttgtt tttggagaac
     1921 ttcttggatt ctgtgcctgg atcgtcctgg gaaactatcg gcgcctggcg cagcgtaata
     1981 ttgccatcgc gttcggcagc gaaaagtcac cacgggaatt gcgccgcctt gtgcgccgtc
     2041 attttcagcg attgggcgcg aaccttttat gcagcgtaaa gatcgcggcc atgccgcttg
     2101 aaagagtccg tgcgcacgtc aagatcgaaa acgcagatgc cgcccatcgc gagttacgcg
     2161 cgggtcgccc cattgttttt gtgttgagtc acctcggcaa ttgggagctg caagcacaga
     2221 tgtttccggt catgatcggt tacgtacgca atagcacgat tttccaaccg ctcggcaacc
     2281 gttacatcga caaacacgtc cgcacactgc gcggccgcgc cggcgtggag ttgttcgatc
     2341 gtcgcgaagg tttccagaaa gccatccaat tgctccgcgg cggcggcgcc atcggcattc
     2401 tgagcgacca acactcgggt gacttgggtc tttgggtccc ttttttcaac aagcttgcat
     2461 cgacatcgcc gctgccgggt cttctcgcaa aacgcaccgg cgccgcgttg ctcggcacgg
     2521 caatttatac cgacgggctg gcccgttggc gaatggtgtt caccgaaagc atcggcgcac
     2581 ttggcgactc catcgaggcg cttacggcca aggcgaacga actgatcgcc gcacaaatcc
     2641 ggcgtgctcc ggaggattgg ttttgggtcc acaatcgctg gaaaataccc gagcccagtt
     2701 tgcttctcgc tcgatacaag cgcggtattt acctgccacc ggatatctcg ggtcaagacc
     2761 tgcagccatt tcgaattctc attcgctcaa gcaattggct cggtgacgcc gtgatgagcg
     2821 tgccggcaat ccgcgcgatt aaaaacgggc gccttgatgc ccatgtcacc attgccacgc
     2881 cagcgaagat tgcgtcaatg tggaaactgg tttcagaagt ggacgcgatt attcctctgt
     2941 cgggcgattc actccttaca accatccgtt tgctccggcg gcaacccagt tttgacgctg
     3001 cgattctctt tcccaattcg ttgcgtgtcg ttcttgaaac atggctcagc ggcattccgc
     3061 gccgggtcgg ttatcgcggg cacgcacgca gctggttatt gaatcagatc atcaccgagc
     3121 cacggcggcc cggcccgctc aaacatcaat ccgctcgcta cctccgaatc gccgaagaac
     3181 tcggtgcaga aattactgcc acgctccggc ttccgccttt ccatcaacaa tcaaccatca
     3241 accatcaacc atggaagctc ggcctctgcc cgggcgcgga atatggcccg gccaaacggt
     3301 ggctgccgga gcgattcgca gaggcggcgg caaaagtcgc cgcgcaagcg ccagtgcagt
     3361 gggtcctttt cgggacgcca aaagatgcgg agattggtga ccagatcgcc gccggcctcg
     3421 gcgattcatg cgtaaatcga attggtcaaa ccacgctcga tcaactcatc cttgccctgc
     3481 gcgagtgccg t
//
LOCUS       DASQUQ010000201         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_142974, whole genome shotgun sequence.
ACCESSION   DASQUQ010000201 DASQUQ010000000
VERSION     DASQUQ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142974"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VE486_05720"
     CDS             complement(<1..796)
                     /locus_tag="VE486_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZA38618.1"
                     /translation="MQTPGQQTVSPARWLADFLGLKRNLVILLVAIFVIGAGEELWMR
                     FVPKYLQAVGATVFVIGLYDALRTLLAAIYAYPGGVLADLWGHRRAFLAFNLISIVGY
                     ALVLFIPHWGAVIAGMFLFLSWTCFSLPATFSLVGATLAASRHSMGVGIQSVIKRLPI
                     MIAPVCGGILIDRFGIIVGVRIALLISIVLSALTILVQRQLREEPKQAVAANERWSFW
                     RSLREFNPSMRRLLLSDILVRFCERIPYAWVVIFAMDYIGVSAKGIG"
     gene            complement(769..1194)
                     /locus_tag="VE486_05725"
     CDS             complement(769..1194)
                     /locus_tag="VE486_05725"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HZA38619.1"
                     /translation="MKWVTREKIKVDRVACPWLIRNFIDSDAEFVFLPRDTNWAKIDN
                     GIVFDVPNCELGHHGEDVSFNSILKKYKLTDPALVLLGEIVRAADSHPRSPHQAGEGL
                     RWIASGFGALGLSDHEILERELVVYDALYAECKRRVSKP"
     gene            1213..1638
                     /locus_tag="VE486_05730"
     CDS             1213..1638
                     /locus_tag="VE486_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZA38620.1"
                     /translation="MAAWFWYAVIAAILYGVHQIFTKLAADHIGEGLGGFVVEATAAL
                     SILIYLAFLWLASRWNQQSSAQGIFYSVLTGVCVGAGTIAFFLLFQKGGPLSSVPAIL
                     AGGAAIMAIAGILFFHEPPSWQRLLGVTFAIIGLLLLRR"
     gene            complement(1635..>2552)
                     /locus_tag="VE486_05735"
     CDS             complement(1635..>2552)
                     /locus_tag="VE486_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZA38621.1"
                     /translation="AALAGKVVGTAIEGEKRHEIVVRMPEQFRADDEKIRELPLRVGE
                     HGLIKLGQVADFKTVKVVEPILRDDGHRRAALLVNLAGHDVEGFVRDAEQRIKQQVKM
                     PEGYLIEFGGQYKNLQEARARLAIVVPAALTLIFILIFLAFGSIRQALLVYSGIPLAV
                     TGGVLALWLRGMPFSITAAVGFIALSGVAVLNGLVMVTYFNQLREQGRTIREAVIEGS
                     LTRLRPVLMTALVASLGFVPMAIATGTGAEVQRPLATVVIGGILSSTFLTLILLPVLY
                     AWFERERTSSAAGVGEIPESQIEPALSRH"
BASE COUNT          591 a          744 c          686 g          531 t
ORIGIN      
        1 tgccaattcc tttcgcgctc acgccgatat aatccatggc gaagatcacg acccaggcat
       61 acggaatgcg ctcgcaaaag cggaccagga tgtcgctcaa aagcaaccgg cgcatcgacg
      121 ggttgaactc gcgcaggctt cgccaaaaac tccaccgctc gttcgccgca actgcttgct
      181 tcggctcttc acgcaattgg cgttgcacga gaatcgtcag tgccgaaaga acgattgaga
      241 tcaggagcgc gatgcgcaca ccgacgataa ttccgaagcg atcgatcaat atcccgccgc
      301 aaactggtgc gatcattatc ggcagtcgct tgatcaccga ttggatgccg acacccatcg
      361 agtggcggct ggccgcgagt gtcgcgccaa ccagtgaaaa ggtcgctggc agtgaaaaac
      421 aggtccagga aaggaataga aacattcccg caatcaccgc gccccaatgc ggaatgaaca
      481 ggaccagcgc gtaaccgacg atggagatta aattaaacgc gagaaatgcg cgccgatgtc
      541 cccaaagatc ggcaaggacg cctccgggat acgcgtagat cgccgcgagc aacgtccgca
      601 gcgcatcgta aaggccgatg acgaaaaccg ttgcgccgac cgcctgcaga tacttcggca
      661 cgaaacgcat ccaaagctct tcgcccgcgc cgatcacgaa aatcgctacg agcaggatga
      721 cgagattacg cttgagcccc aggaaatcag caagccagcg cgcaggactc acggtttgct
      781 gacccggcgt ttgcactctg cataaagcgc gtcgtaaaca acgagctcgc gctcaaggat
      841 ttcgtgatca cttaatccaa gcgcgccgaa cccgctggca atccagcgca atccttcgcc
      901 ggcttgatgc gggctgcgcg ggtgcgaatc ggctgctcgg acaatttcgc cgagtagaac
      961 gagcgctgga tcggtgagct tgtacttttt taggatcgag ttgaacgaaa cgtcttcgcc
     1021 atgatgacca agctcgcaat tcggcacgtc gaacacgatg ccgttgtcga tcttggccca
     1081 atttgtgtca cgcgggagga agacaaactc agcatcggag tcgataaaat ttcggatcag
     1141 ccacggacaa gcaacacggt ccactttaat tttctcgcga gtgacccatt tcataagcta
     1201 gattcttgtt tcgtggcagc gtggttttgg tacgcagtta tcgccgctat tctttacggc
     1261 gtacatcaaa tctttaccaa gctcgcggcg gaccacatcg gcgaaggttt gggcggattc
     1321 gttgtcgaag ccaccgccgc attgtcgatc ctaatttatc tcgcgtttct ttggttggcg
     1381 agccgttgga atcagcaatc cagtgcgcaa ggaattttct attctgttct caccggcgtc
     1441 tgcgttggtg cgggcacaat tgcattcttc ctactttttc agaaaggagg accgctctcg
     1501 tctgttccag ccattttggc gggcggtgcg gcgatcatgg ccatcgccgg cattctgttc
     1561 tttcacgaac cgccttcatg gcagcggctt ctcggcgtga cattcgcgat aatcggattg
     1621 ctgctgctcc gacgttaatg gcgggaaagc gctggttcga tctgggattc cggaatttcg
     1681 ccaacgcctg cagccgacga cgttcgttcg cgctcgaacc aggcgtacaa caccggcagc
     1741 aagatcaacg tcaaaaatgt tgagctaaga attccgccga tgacgacagt ggcaagcggt
     1801 cgttgaactt cggcgccagt gccggtggcg atcgccattg ggacaaaacc gaggctcgca
     1861 actagggctg tcatcagcac gggacgcaac cgcgtgagcg aaccttcaat gactgcctcg
     1921 cgaatcgttc gaccttgttc tcgcagctgg ttgaagtagg tgaccatgac gagcccgttt
     1981 aaaacggcga cgccgctcaa tgcgatgaat cccacagcgg ccgtaatact gaacggcatt
     2041 cctcgcagcc agagcgccag aacaccaccc gtcactgcaa gcgggatgcc ggaataaacg
     2101 agcagagcct ggcgaatgct gccaaacgca agaaagatca agatgaaaat caacgtcaac
     2161 gccgcaggca cgacgattgc cagtcgcgcg cgcgcttctt gtagattttt gtactggccg
     2221 ccgaattcga tgaggtagcc ttcgggcatt ttcacctgct gtttaatccg ttgttccgca
     2281 tctcgcacga aaccttccac gtcgtgcccg gcgagattca ccaacaaagc ggcgcgccga
     2341 tgaccatcgt cgcgcagaat cggctcaacg actttgaccg ttttaaaatc ggcaacttgc
     2401 ccgagcttga tcaggccgtg ctcaccgaca cgcaacggca actctcgaat cttttcgtcg
     2461 tccgcccgga attgttccgg cattcgcaca acgatttcgt gccgtttttc gccttcaatc
     2521 gcggtgccga cgactttccc ggcgagtgcc gc
//
LOCUS       DASQUQ010000202         4920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_954657, whole genome shotgun sequence.
ACCESSION   DASQUQ010000202 DASQUQ010000000
VERSION     DASQUQ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954657"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VE486_05740"
     CDS             <1..227
                     /locus_tag="VE486_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38622.1"
                     /translation="REEVLPNSCRKRVAIEAGVTEIWYQYVGLDGKVVGLHEFGLSAP
                     GAEVMKERGIDAEHVVEAAKSLLANSQNSG"
     gene            complement(385..1875)
                     /gene="dnaB"
                     /locus_tag="VE486_05745"
     CDS             complement(385..1875)
                     /gene="dnaB"
                     /locus_tag="VE486_05745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZA38623.1"
                     /translation="MPGSQGSQPGKPRKTAGNGAGRGSAAGNGGRVFPNSPTGSAQDI
                     HRTPPHSVEAEQGVLGSMLISPREAIAECVEKINEEYFYVPAHQTIYNVLVDLWNAGQ
                     AIDLITFTQVLRDRNLLESVGGAAFVTSLFTFVPTAANVQYYLEIVRDKYILREIISA
                     ATESVRRAYEEQDEVNNLLDEVEQKIFAVGEDRFKGQMLSMKDQVMEAIESIEKLYER
                     KGGITGISTGFVEFDRMTSGLHPSEMIVIAARPSMGKTALAMNIAEHVAINEMLPVGV
                     FSLEMSSQQLVQRLLCSRARVNLQKVRDGFLSERDFPSLTAAASKLAEAKIFIDDSAS
                     LTILELRAKARRLKAQQDVQLLIVDYLQLLRSTSRRAQDNRQLEISEISAGLKGLAKE
                     LKIPIIVVAQLNRQPEQRSGGKPRLSDLRESGSIEQDADLVGLLVRPEIYEEDEEARA
                     EKAGEAEFIIAKQRNGPVGEIPLTFLKEFTRFETRARNVSEPEEAF"
     gene            complement(1876..2421)
                     /gene="rplI"
                     /locus_tag="VE486_05750"
     CDS             complement(1876..2421)
                     /gene="rplI"
                     /locus_tag="VE486_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZA38624.1"
                     /translation="MPLTEIILTENVPGLGAEADVVKVRRGYARNYLLPHGKAHEVTP
                     ASLRQIDSLKAKRAEREARELNDAEELARRIGKARITFTLETGETGKAFGSVTAQDIV
                     NRLKNELGAEIDRHKIALERPIKDTGEHEVAIKLHRDVTAQFVFQVKSAEKPKTEAVS
                     AAAEEPEKKHHRFFKKRKETK"
     gene            complement(2557..3888)
                     /locus_tag="VE486_05755"
     CDS             complement(2557..3888)
                     /locus_tag="VE486_05755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZA38625.1"
                     /translation="MHAISSKKTIEERTHRVLSQIFAGCSLEKVGVRLWDGTSWPDEQ
                     PRKATLVLKYPGALSRMFLPGTEVGLAEAYLQDDFDIEGDIEGAFEVGDFLLSRLDDW
                     KKKLKLGGLLIALPERDGTSSMRRAARQLLSRVRDKRHSPERDRRAVTFHYDVSNDFY
                     RLWLDRQMLYSCAYFQSPRDDLDAAQEQKLDYICRKLRLRAGQRLLDIGCGWGALVLH
                     AAEHFGVRADGITLSESQAKRGRARIAETGLGRNAKIELRDYREIGAESRELYDAIVS
                     VGMAEHVGRERLPDYFAAAHRALKPGGVFLNQAIGEDIIARPGKPEGFGGSFIEQYVF
                     PDGDIPPLPIMLRAAESSGFEIRDVENLREHYALTLRHWLRRLEAHHDEALRHVDETT
                     YRIWRLYIAGSAHGFRSGHIAVYQTLLAKLDPSGRTNLPLTRDDLYTQNHR"
     gene            complement(3911..4465)
                     /gene="sufT"
                     /locus_tag="VE486_05760"
     CDS             complement(3911..4465)
                     /gene="sufT"
                     /locus_tag="VE486_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HZA38626.1"
                     /translation="MYENTEFTLSRDCEAIQIPSGQKTTIPAGTQGVITQSLGGSYTV
                     ATYQGLARVAEKDLDALGLEKPQTQAAQKTASTANGEVSEEHVWNQLRQCYDPEIPVN
                     IVDLGLVYDCRLIKKDGGGTKVEVKMTLTAPGCGMGPAIAHDAQSKILSIDGVDEADV
                     QLVWDPPWNQNMISEAGRMKLGMI"
     gene            complement(4611..>4920)
                     /locus_tag="VE486_05765"
     CDS             complement(4611..>4920)
                     /locus_tag="VE486_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38627.1"
                     /translation="RDEFEAFQKEGILTRLDCAWSRDQPEKFYVQHCLMENAAEIWKW
                     LDGEGAHFFVCGDARRMAKDVDATLRKIVQAQGGKTFDEANEYVEKLKSDKRYKRDVY
                     "
BASE COUNT         1014 a         1366 c         1308 g         1232 t
ORIGIN      
        1 atcgcgaaga ggttctcccg aactcctgtc gcaagcgtgt cgcgattgaa gctggcgtta
       61 ccgagatttg gtatcagtac gtcggtctcg atggcaaagt tgttgggctg cacgagtttg
      121 gcttgagcgc tcccggcgcc gaagtgatga aagagcgcgg gatcgacgcc gagcacgttg
      181 tcgaagcggc gaaatctttg ttggcaaact cgcaaaactc cgggtagcgc acgcgtcttg
      241 cgtgctgacg atggcgtcct cgccatcgcg aacttttttt gtgagttcga cccgcctgca
      301 aggagcgaaa ttttgaggaa agactgtttc ggcgcgacgc cgaaaccagc acgcgagacg
      361 cgtgcgctac ccagaaggct gcgtctaaaa cgcttcttct ggctcgctca cattgcgcgc
      421 ccgagtttcg aagcgggtga actcttttag gaacgtgagc ggaatttcac ccacaggacc
      481 attgcgctgc ttggcgataa tgaattcggc ttctccggct ttttccgcgc gcgcttcttc
      541 gtcctcctcg taaatttccg gcctgacaag taatcccacc aggtccgcat cctgttcgat
      601 tgagccggat tcgcgcagat cgctcaagcg cggcttgccg ccagaacgct gttctggttg
      661 tcgattcaat tgcgccacca cgatgatcgg aattttcaat tccttcgcca aacctttcag
      721 gcccgcggag atttccgaga tttccaactg ccgattgtcc tgcgcccggc gcgatgtgga
      781 tctgagtagt tgtaaataat cgacgatgag cagttgcaca tcctgctgcg ctttcagccg
      841 gcgcgctttc gcgcgcaatt cgaggatcgt gagactcgcg ctgtcgtcga tgaaaatttt
      901 tgcttcggct agtttcgatg ccgcggctgt taaactcggg aaatcgcgtt cgctcaaaaa
      961 tccgtcacgc actttttgca aattgactct cgcccgcgag caaagcagac gttggacgag
     1021 ctgctgactg ctcatttcca ggctgaacac gccgacaggc agcatttcgt taatggcgac
     1081 gtgttcggcg atattcatgg ccagcgccgt ttttcccatg ctcggccgtg ccgcgattac
     1141 gatcatttcg gagggatgca agccgctggt cattcgatcg aactcgacaa agccggtgga
     1201 gatgccggtg atgccgcctt ttcgttcgta gagcttctcg atcgactcga tcgcttccat
     1261 cacttgatcc ttcattgaaa gcatctggcc tttaaaacgg tcttcgccga ctgcgaagat
     1321 tttctgttcc acttcgtcga gaagattatt cacctcgtcc tgctcttcgt aagcccggcg
     1381 cacgctctcc gtggcggcag agatgatttc ccgaaggatg tatttgtcgc ggacgatctc
     1441 cagatagtac tgcacgttgg cggccgttgg gacgaacgtg aacaggctcg tgacgaaggc
     1501 cgccccgccc acgctttcga gcaaattccg atcacgcaag acttgcgtga atgtgatcag
     1561 gtcgatcgcc tggcccgcat tccaaagatc gacaagaacg ttgtaaatcg tctggtgcgc
     1621 cgggacgtaa aagtattctt cgttaatttt ctcgacgcac tccgcgatgg cttcacgcgg
     1681 tgagatgagc atcgagccca agacgccttg ctctgcttcc acgctgtgcg gaggcgttcg
     1741 gtgaatatcc tgcgcagaac ctgttggcga attggggaaa actcttccgc catttcccgc
     1801 cgccgaacct cggccagcgc cattacccgc tgtcttacga ggtttcccag gctgagagcc
     1861 ttgagaaccc ggcatttatt tggtttcttt tcgtttcttg aaaaatcgat gatgtttttt
     1921 ctcaggttcc tcggccgcag cactcactgc ctcggtcttt ggtttttctg cggatttcac
     1981 ctgaaaaaca aattgcgcgg tcacgtcgcg gtggagcttg atcgccactt cgtgctctcc
     2041 tgtatctttg atcggtcgct cgagagcaat tttgtgccga tcaatctccg cccccagctc
     2101 gtttttaagc cggttcacga tgtcctgtgc ggtgaccgag ccgaaagctt tgccggtctc
     2161 accggtttcc agcgtgaagg taattcgcgc tttgccaatt cgacgagcga gttcttcggc
     2221 gtcgttcaat tcgcgtgctt cgcgttcggc gcgtttcgct ttgagcgaat cgatttgccg
     2281 caagctcgct ggcgtgactt cgtgcgcttt gccatgcggc aataaatagt tgcgcgcgta
     2341 accgcgccgc actttgacca cgtcggcttc cgcgcctaaa ccgggaacat tttcggttaa
     2401 gatgatttca gtcaaaggca ttggtaatgg ttatctgaga gaaatttttg tgcgataaaa
     2461 gccgagagga aggcgagtat agaagccgag agcgcggtgt caactcgcga aaattttcgg
     2521 agctaattcc gcaagagaat ttactgatgt cgcgcttcat cggtggtttt gcgtgtacaa
     2581 atcgtcgcgc gtgagaggaa ggtttgtccg gccggacgga tccagctttg cgagcagcgt
     2641 ttggtaaaca gcaatatgac cgcttcgaaa tccgtgtgcc gagccggcaa tgtagagacg
     2701 ccagattcga tatgttgttt cgtcgacgtg gcggagcgct tcatcgtgat gcgcttccaa
     2761 tctgcgaagc caatgtcgca gagtgagggc gtaatgttcg cgcagatttt ctacatcgcg
     2821 gatttcgaaa cctgacgact ccgcggcgcg cagcatgatc ggcaggggcg gaatatcgcc
     2881 atcgggaaaa acatattgtt cgatgaatga gccgccaaaa ccttcgggct tgcctgggcg
     2941 tgcgataata tcttcgccga tcgcttgatt aagaaaaaca ccgcccggct tgagtgcccg
     3001 atgggcagcc gcgaagtaat ccggcagccg ctcgcgtccc acatgttctg ccattccaac
     3061 acttacgatt gcatcataaa gttcgcggct ttctgcgccg atctcgcgat aatcgcgcag
     3121 ttcgatcttc gcgttgcgtc ccaatccagt ctcagcaatt cgtgcccggc cccgtttcgc
     3181 ttgcgactcg cttagagtga ttccatcggc gcgcacaccg aaatgctccg ccgcatgaag
     3241 gacaagtgcg ccccaaccac agccaatgtc gagcagacgt tggccggctc tgagtcgtaa
     3301 tttacggcag atgtaatcaa gtttttgttc ctgagctgcg tcgaggtcgt cgcgcggcga
     3361 ttggaagtac gcgcacgagt aaagcatttg ccgatccaac cagagacgat aaaaatcatt
     3421 cgaaacatcg tagtggaatg tgacggcacg ccgatcacgc tcgggtgagt gacgtttatc
     3481 acgaactcgt gacagcagtt gtctcgcggc acgccgcatt gatgatgttc cgtcacgctc
     3541 gggaagcgcg atgaggaggc caccgagctt cagctttttc ttccaatcat cgaggcggct
     3601 aagcaagaaa tcaccgactt caaatgcccc ctcgatatca ccttcgatgt cgaagtcgtc
     3661 ctgcaaatat gcttcggcca aaccgacttc ggtcccgggc aagaacattc gggagagcgc
     3721 gccgggatat ttcagcacga gggtggcctt gcgcggctgt tcgtccggcc atgaagtgcc
     3781 atcccacagt cgcacgccga ccttctcgag cgagcagccg gcgaaaattt ggcttagaac
     3841 acgatgcgtt ctctcctcga ttgttttttt cgaggagatc gcgtgcatcg cggcatgaca
     3901 aaacgcgagg ctaaatcatc cctagcttca tgcgacccgc ttcgctgatc atgttttgat
     3961 tccagggcgg atcccaaacg agttggacat cggcttcatc gaccccatcg atggacagaa
     4021 tcttgctctg cgcatcgtgc gcgattgccg gtcccatccc acagcccggg gcggtgagtg
     4081 tcattttcac ttccactttc gtgccgccgc cgtctttttt gatcaaccgg caatcgtaaa
     4141 cgaggccgag atcgacaatg ttcaccggga tttccgggtc gtaacattgg cgaagctggt
     4201 tccagacgtg ttcttctgaa acttcaccat ttgcagtgct cgcagttttt tgagcggctt
     4261 gggtctgcgg tttttctaag ccgagagcgt cgagatcctt ctcggcaacc cgcgccaacc
     4321 cctggtaggt ggcaacagtg taacttcccc caaggctctg cgtgatcacg ccctgtgttc
     4381 cagccgggat cgtcgtcttt tgcccactgg gaatttggat cgcctcacaa tcgcgactca
     4441 atgtgaattc ggtgttctcg tacatggttt taacgttgac agtctactat gccacaaatg
     4501 aagaaaatga cgaaatccga atgacgaatg aggaaataat gtcgaaaccc aaatgtcgaa
     4561 tgaatcacag gccttttttt cgatttgatt attcattcct ccttcgtcat tcaataaacg
     4621 tcgcgtttgt agcgcttatc gctctttaat ttttcaacgt attcgttagc ctcatcgaag
     4681 gtcttgccgc cttgcgcctg cacgatcttg cgcagggttg catccacgtc cttggccatc
     4741 cggcgcgcgt cgccgcacac aaaaaagtgg gcgccttccc catcaagcca tttccagatc
     4801 tcggcggcgt tttccatcag acaatgttgc acatagaatt tctcaggttg atcgcgtgac
     4861 caggcacaat cgagtcgggt tagaattccc tctttttgga acgcctcgaa ctcatcgcga
//
LOCUS       DASQUQ010000203         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_520124, whole genome shotgun sequence.
ACCESSION   DASQUQ010000203 DASQUQ010000000
VERSION     DASQUQ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520124"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VE486_05770"
     CDS             <1..825
                     /locus_tag="VE486_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZA38628.1"
                     /translation="GMIAPRTPTLSYGLRGVTALEIKVTGPKMDLHSGIFGGAVANPI
                     TALAQLLATLHDREDRVAIAGFYDRVKPIEEWEREAWRKLPVDGDKLILEETGVPALF
                     GEAGYNSLERIWARPTAEINGIGGGYQGQGTKTVIASHAMAKLTFRLVPEQEGDEILK
                     LAKTHLRKNLPKGVTLEMTDGHSGPWYLTDPHSQIGESAQRALRKAFNADAALIREGG
                     SIPIVSQFRSILGIETLLMGLALPDCRAHSPNENFPLENLEGGIRMNKAILQELAH"
     gene            1416..1928
                     /locus_tag="VE486_05775"
     CDS             1416..1928
                     /locus_tag="VE486_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HZA38629.1"
                     /translation="MRQLIEAGLELTKQNLGYTYGSADPTNGGMDCSGFVYYVLQQNG
                     FPDVPRDSSQQYVWVRKAGNFNAVLSRNEDSFELDALKPGDLLFWRGTYNIDRDPPIT
                     HVMIYLGREKGTKKRVMVGASDGRTYDGKQRFGVSVFDFKMPPPPNSGDAKISPVFVG
                     YGRIPGLSED"
     gene            complement(2090..2281)
                     /locus_tag="VE486_05780"
     CDS             complement(2090..2281)
                     /locus_tag="VE486_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38630.1"
                     /translation="MKRLLRIFVLSKSEQRVVLIVLLALVAGAFVRYERSVHRVPVQQ
                     ASATEPKASPSPPGPEEEQ"
     gene            complement(2278..3051)
                     /gene="queC"
                     /locus_tag="VE486_05785"
     CDS             complement(2278..3051)
                     /gene="queC"
                     /locus_tag="VE486_05785"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZA38631.1"
                     /translation="MKLVGRDSVEPTTHEGHSTAPQSVVLPNGDKRAVVLLSGGVDST
                     TTLAIGITEGYEAYALSLDYGQRHQLEIDAARRVANSLGAKEHRVAKIDLRVFGGSAL
                     TDDVDVPKHRSETEIAHGIPVTYVPARNTIFLSYALAWAEVISANHIFLGVNAIDYSG
                     YPDCRPEFIKTFEKLANLGTKAGVEGKRFQIHTPLIKFSKADIIRKAVELGVDLSLTH
                     SCYDPSAEGRACGECDSCLLRLKGFREAGIKDPIRYVKK"
     gene            complement(3048..3482)
                     /gene="queF"
                     /locus_tag="VE486_05790"
     CDS             complement(3048..3482)
                     /gene="queF"
                     /locus_tag="VE486_05790"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZA38632.1"
                     /translation="MTKTKTSRLTLLGRSEVKLPASPAEARLETFPNPARRNYRIHFE
                     TDDFTSVCPITGQPDFARIDIDYMPNRLCVESKSLKFYLASYRNERAFNEAVTNRILD
                     DFVKACAPREVIVTAQFSARGGIALTVRAEYPDKSRSSRDGR"
     gene            complement(3654..4199)
                     /locus_tag="VE486_05795"
     CDS             complement(3654..4199)
                     /locus_tag="VE486_05795"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZA38633.1"
                     /translation="MHEGGFDVQEKEMVDDLTADQVRGLLKLELHATCGFVRVTFISE
                     KRIAPGGMPPPFANGRPAGSALYFMVTPDARVRLHRIRNDQLYHYYLGDPIEVLMLHA
                     DGTSEHAIVGPDLRGGQLVQLLIPGNTFHTARVIGRRRWFLGASTEWPGVEPADVEIG
                     DVEALAAKYPDVADDLRAFSG"
     gene            4194..4631
                     /locus_tag="VE486_05800"
     CDS             4194..4631
                     /locus_tag="VE486_05800"
                     /inference="COORDINATES: protein motif:HMM:NF042458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6572 domain-containing protein"
                     /protein_id="HZA38634.1"
                     /translation="MQLRRGGRRTPKIFARKLPFFQRSIVWMDIKHQKSKINNRVGVI
                     DVIAQDSNTGEVVLVMNEPEPWDDSDERLLALQERFNAYVSFLLDGEMASEHPELAGK
                     PARIELRCVHIPDTRALELLGMIHDQLAFQEIRMEVVVAETQP"
     gene            4710..>5507
                     /locus_tag="VE486_05805"
     CDS             4710..>5507
                     /locus_tag="VE486_05805"
                     /inference="COORDINATES: protein motif:HMM:NF018963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HZA38635.1"
                     /translation="MQIWKVLVPVFLIYSGAFAVESRLPFSTVFKGQDQFNRLVAKAK
                     SGNWEALPIGDRTAAVGLALVGTRYKHYTLEIDNRIESPSVNFNGMDCWTFFEIALGF
                     ARMLNEPESTWTPERLMHYIELDRYRGGECTGEYLSRLHYLEDWLYDNDRRGLVEDMT
                     RSLGGVSVAHSAREMSVGWRHYRYLAANRSLLGPLARMEANVSSRPLYQIPKNRVARI
                     EPKLRNGDIIGIIGREGRGRYSTSHVGLAVRTKDGGLHFLHASSPSNY"
BASE COUNT         1230 a         1550 c         1563 g         1164 t
ORIGIN      
        1 ggaatgatcg cgccccgaac gccaacccta agctacggac tgcgcggcgt gacggccctc
       61 gaaatcaaag tcactgggcc gaagatggat ttgcactccg gcatatttgg cggcgcagtt
      121 gcaaatccaa tcaccgcact cgcgcaatta ctagctaccc ttcacgatcg cgaggatcgt
      181 gtggccatcg ccggatttta cgatcgtgtg aagccgatcg aagagtggga gcgcgaagcg
      241 tggcgcaaac ttccggtcga cggcgacaaa ctaattctcg aggaaacggg tgtgcccgcg
      301 cttttcggcg aggctgggta taattcgctg gagcgcattt gggcgcgacc aaccgccgag
      361 atcaatggaa tcggcggcgg ttaccagggg caaggaacca agacggtcat cgccagtcat
      421 gcgatggcga aactgacatt ccgtctcgtt ccggaacagg aaggtgacga aattctaaaa
      481 ctcgcaaaaa cgcatttgcg caagaatttg ccaaaagggg tcactcttga aatgacggat
      541 ggccacagcg ggccgtggta tctgactgat ccgcattcac agatcggtga gtcggcccag
      601 cgagctttgc gcaaggcgtt caatgcggac gccgcgctca ttcgtgaagg tggcagcatc
      661 ccgattgtct cgcagtttcg cagcatcctg ggcatcgaga cactgctgat gggactcgcc
      721 cttcccgatt gccgcgcgca ctctcccaac gagaatttcc ctctcgaaaa tctcgaaggt
      781 ggcattcgca tgaacaaagc aattctacag gagctcgccc actgattttg tttccagtaa
      841 ccaaaagacc tcccaccaag gttagaggca ccgacgcaaa ctctagggcg tgtgtgtcag
      901 acgccggggc aattggcgtt tcacagaaac gccctacaaa gcggacctcc aaaacaacgt
      961 ttcgaatctt tctaatcgcc gcgatctgcg ccggcgcgat cgtcactctg agcagggcgg
     1021 aagagccgtt aggcagaaag attaagaaat ttttcaccac aaccccaacc ccgactccag
     1081 cgcgaaaaca ccggaagcga tcggcgaaga aacccacgcc tagtccagca cctactgctt
     1141 caccaaaacg gaagaaggcg tcgcccaccc catctccaag cccgacgccg gaaacagaac
     1201 ggaagcgcaa gaaagggtct ccgactccta cgccaggcgc atcgccatcc gagacagtcg
     1261 cgcccgagga agcgccatcg ccgagtccga ctgaaagtcc gcaacccact gcgatacctt
     1321 caacgacccc gcatcctacg ccgggaaaaa agggcgcgcc caacgttacc gttccgcctg
     1381 atgagataac gggcttcgat actgatcccg cgaaagtgcg tcaactcatc gaggccgggc
     1441 ttgagctgac gaaacagaat ctcggttaca cctacggctc cgccgatccc acaaatggcg
     1501 gcatggattg ctccggtttc gtttattacg ttctccagca aaacggattt cccgatgtgc
     1561 cgcgcgattc gagccagcag tatgtctggg tgcgcaaggc gggaaatttc aatgcggtct
     1621 taagccgtaa cgaagattca ttcgaactcg atgcgcttaa gccgggcgat ttgcttttct
     1681 ggcgcggcac ctacaacatc gatcgcgatc cgccgatcac gcacgtgatg atttatttgg
     1741 gccgggaaaa gggaacgaag aaacgggtga tggtcggggc gagcgacggt cgcacttacg
     1801 atggtaaaca gcgctttgga gtcagcgttt tcgatttcaa aatgccaccg ccgccgaata
     1861 gtggcgacgc caaaatcagt ccggtgtttg tcggttacgg gcgtattccc gggctgagcg
     1921 aggactgatt ccggtagcgc acgcgtctcg ggtgctgggg actgcgtcct cgcgatcccg
     1981 gactctttct gtggtaccct cggtcgtggg ccatcgttag cgttgggaaa gactgtttcg
     2041 gctcgacgcc gaaaccagca cgcgagacgc gtgcgctacc cggaagcgat tattgctcct
     2101 cctcgggtcc gggaggactc ggagatgcct tcggctccgt tgcgcttgcc tgctgaaccg
     2161 gaacccgatg gacgctccgc tcatatcgca cgaacgcgcc ggcgaccaac gcaagcagca
     2221 caatcaggac aacgcgttgt tcacttttcg aaagcacgaa gattctgagc aggcgtttca
     2281 ttttttcacg taacggattg gatcttttat tccggcttcg cgaaaacctt tcagccgcaa
     2341 aagacacgaa tcacactcgc cgcaggcacg gccttccgcc gatggatcgt agcaactgtg
     2401 ggtgagcgag agatcgacgc cgagctcgac agctttgcga atgatatcgg ccttcgaaaa
     2461 cttgatgagc ggcgtgtgaa tttgaaaacg cttgccttca acaccggctt tggtgccaag
     2521 attggctagc ttttcgaatg ttttgatgaa ctcaggccgg cagtctggat agccgctgta
     2581 gtcgattgcg tttacaccga gaaaaatgtg gttggccgaa atcacttccg cccaggcgag
     2641 cgcgtaagag agaaaaatgg tgttgcgtgc cgggacgtaa gtgaccggaa tcccgtgcgc
     2701 gatttctgtt tccgatctgt gtttgggaac atcgacatca tccgtaagcg ctgagccgcc
     2761 gaagacgcgc aggtcgatct ttgccactcg atgctctttt gcgcctaatg aattagcgac
     2821 ccgacgcgca gcgtcgatct cgagctgatg acgctggccg taatcaagcg acaaggcgta
     2881 cgcttcgtag ccttccgtaa tcccaatcgc cagcgtcgtc gtcgaatcaa ctccgccgct
     2941 tagaaggacg actgcgcgct tatctccatt tggtaggacg acgctctgcg gagccgtcga
     3001 atgcccttca tgtgtcgtcg gctcgacaga gtctcgcccc accagtttca tcggccatcg
     3061 cggctacttc tgcttttatc cgggtattca gcgcgcacgg tcagcgcgat tccgccacgc
     3121 gcggagaatt gagcggtcac gatgacttcg cgcggcgcgc acgctttcac aaaatcgtcc
     3181 aggatgcggt tcgtgaccgc ttcgttgaac gcacgctcat tccgatacga ggcaagataa
     3241 aatttgagcg atttgctttc gacgcagagc cgattcggca tgtaatcgat gtcgatcctg
     3301 gcaaaatccg gctgacccgt gatcgggcag acggaagtga aatcatccgt ttcaaaatga
     3361 atccggtagt ttcgacgcgc cggatttggg aaagtctcga gccgcgcctc cgcaggcgac
     3421 gcgggaagtt tcacttcgct ccgcccgagg agggtcagtc tggacgtttt ggtcttcgtc
     3481 acggaaatga attaactcgt cactcgtgca gcgttcaact cgcgagtttc gggtagcgca
     3541 tgcgtcccgc atgttggttt cggcgtcgcg ccgaaacaat cttttcgaag aaactcatag
     3601 atcggcatga atttcacgta tccagaaaag tccgcgatcg cgagacgcat gcgctaccca
     3661 gagaatgcgc gcagatcgtc agcgacgtct ggatatttcg ctgccagcgc ttcgacgtcg
     3721 ccaatttcga cgtcagccgg ttctacaccg ggccattcgg tgctggcacc gagaaaccag
     3781 cgccgccgcc cgatcacacg cgcagtgtgg aaggtattgc cgggaatcaa aagttgcacc
     3841 agctggccgc cacgcaggtc tggccctacg atggcgtgct cgctggtgcc gtcagcgtgg
     3901 agcattaaga cttcgatcgg atcgcccagg taataatgat aaagctggtc gttgcggatg
     3961 cgatgcagcc gcacgcgcgc gtccggcgtc accataaaat agagcgcgga accggcaggc
     4021 cgcccattgg caaatggcgg gggcattccg ccaggcgcga tccgcttttc acttatgaaa
     4081 gtcacgcgca caaatccgca tgtagcatgc agctcgagct tgaggagccc gcgaacctga
     4141 tctgcggtta aatcatcgac catttctttc tcttgaacat cgaacccgcc ttcgtgcaac
     4201 tacggcgtgg cgggcgccga actccgaaaa tctttgcgcg caagcttcca ttcttccaac
     4261 gttcaattgt ctggatggac atcaaacatc aaaaatcaaa aatcaataat cgagtcggcg
     4321 taatcgatgt catcgcgcag gattcaaata ctggcgaagt cgtcctagtg atgaacgaac
     4381 ctgagccgtg ggacgattcc gacgaacggt tgctcgcgtt acaggaacga ttcaacgctt
     4441 atgtttcgtt tttgctcgac ggagaaatgg cgagcgaaca tcccgagcta gctggcaaac
     4501 cggcgcgaat cgagctgcgt tgtgttcaca tccccgacac gcgcgcgctc gagttgctcg
     4561 gcatgataca cgatcagctt gcgttccagg aaataagaat ggaagtggtc gtggccgaaa
     4621 cgcagccgta gcggcggtcg gccactatca tacccccgaa cgctttcggg gttgatcgga
     4681 gatgagtttc gcgttgagtt gttcgccata tgcagatttg gaaagttttg gttccggtct
     4741 ttttgattta ctcgggcgcg ttcgcggttg aatcgcgcct gccgtttagc acggttttca
     4801 agggacagga tcaattcaat cgcctggtcg cgaaggccaa atccgggaac tgggaggcgt
     4861 tgccgatcgg cgaccggacc gccgcggtcg gactagcgct ggttgggacg cgctacaaac
     4921 attacacgct cgagatcgat aaccggattg agtcgccttc ggtgaatttc aacgggatgg
     4981 attgctggac gtttttcgaa atcgcccttg gcttcgcccg gatgttgaac gagccggaat
     5041 cgacttggac cccggaacgt ttgatgcatt acatcgagct ggaccgttac cgtggtggcg
     5101 aatgcacggg cgaatacctt tcgcgtctgc attatttgga ggactggctt tacgacaacg
     5161 atcgccgggg cttggtggaa gatatgacgc gcagtctcgg cggggtgagc gttgcgcatt
     5221 cagcgcgcga gatgagtgtc ggctggcggc attatcgcta ccttgcggca aatcgttcgt
     5281 tgctcggacc gctcgcgcgg atggaagcga acgtttcttc gcgtccgctc tatcaaattc
     5341 cgaaaaaccg ggttgcccga attgaaccga aactgcgcaa cggcgacatc atcggaatta
     5401 tcgggcgtga aggacgcggg cgttactcaa catcgcatgt tggcctcgcg gtccggacca
     5461 aagatggcgg tttacatttc ctgcacgctt cttcgccgag taattac
//
LOCUS       DASQUQ010000204         6301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_520159, whole genome shotgun sequence.
ACCESSION   DASQUQ010000204 DASQUQ010000000
VERSION     DASQUQ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520159"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1041
                     /locus_tag="VE486_05810"
     CDS             319..1041
                     /locus_tag="VE486_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZA38636.1"
                     /translation="MKILTLLIVCFASVALAEDFKAIDGKEYKNVKLSRIEPDGIVLI
                     SKSGISKVYFSELPKEVQDRFHYDAAQAAAYSAEQTASQVALQNQQAELRRKLAEEKN
                     RYWIGQDPAKNQKDKLPASAAVVADRGKQVEVISHGAPVDITKHLALGKVTVVDFYAD
                     WCGPCKHLSPSLEQMARTDPEIALRKIDIVNWKTAVVKQFNIRSIPQVNVYNRGGRLV
                     GTVVGSDVGEIKRYITQAKSGG"
     gene            complement(1130..1594)
                     /locus_tag="VE486_05815"
     CDS             complement(1130..1594)
                     /locus_tag="VE486_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38637.1"
                     /translation="MKTKTIGLTLAFCFLGSAACLAADPQMGTWKLNESKSKIAPGTL
                     KNTHVVYSSMLGQVTVKSDGIDGEGKPTHIEWSGKFDGTDHPLTGDPTSDTRAYRKID
                     DRTLEVTAKKNGKVTATARIVVAADGKSRTVTVTGTTPKGKKFKNVAVYDKA"
     gene            complement(1861..2778)
                     /locus_tag="VE486_05820"
     CDS             complement(1861..2778)
                     /locus_tag="VE486_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZA38638.1"
                     /translation="MRMSLSQKCLVLLLGAALLGGGAYFARIVFRPQGLERYKSIPWV
                     TYVHPNVKKLKQAQALVREEKLDEARAILVNALITAPKSPVTRELRDLLGEVNTQIFF
                     SKEASPRKTEYTVKRGDALSSIARKLESSTEMIVRVNDLDSTLIRPGEKLLVPQLDFT
                     ITIDLLRDRVVVHDGRGFFAQYPIASADLPASRKPAIQTKVAAKSFWENGRPVRSSRP
                     LQKEGTPRIDLGRPGYVLYGVEEDSETSSSEIAVEEDNAETIADSRDADRPPQGIAML
                     KDDIAEIDLLIRKGAPVTIIRDHQSDDHN"
     gene            3026..3481
                     /locus_tag="VE486_05825"
     CDS             3026..3481
                     /locus_tag="VE486_05825"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZA38639.1"
                     /translation="MNWFFRTLLVLLAIPSLSIGGQSAEQQATINRSATSGELFDTIA
                     RMDSAIFDAFNKHDVDRLMSMFTDDLEFYHDTGGLTNYQQSQESFKKMFASTPDIRRD
                     LVKGSPEIFPIKDHGAIEIGEPRFCHRENGKDDCGTFKFAMIWRKNGDS"
     gene            3546..5153
                     /locus_tag="VE486_05830"
     CDS             3546..5153
                     /locus_tag="VE486_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZA38640.1"
                     /translation="MKSKYDVVILGAGHNGLVAASYLARAGLSVLLLEKNDYIGGATT
                     SQKVFPDYEARLSRYSYLVSLFPEKIIRDLGLNLELRRRATASFTPYIQDGRHNGLLL
                     SNVSEEMSRQSVFELTGNYIEYEQMKKFYNLSRVFAEHVWDTMLEPLVAKEELRRRFD
                     VDEVSREAWRSLAEEPLGRAIERYLQNDLVRGLVLTDGKIGVSTYPHDPSLLQNRCFL
                     YHLIGNKTGEWKVPAGGMGAVARELEQAARKAGAESLTSVDVRALDLKGEKKTVEFEI
                     EAAGGRTFAPHSETVEARYLLVNFGRNVLAKFTGKLYQPQAEDEGSVFKINMLLRRLP
                     KLKAARYAATEAFCGTFHCDEGYEQMNVSYQQATEGRLPNKIPCELYCHTLTDDSILA
                     PELREKGFHTLTLFGIDTPWGLFARDNRTMRELAEKKFIEGMNQWLEEPLEDCFAVAR
                     DGSLCIESKSPVDIEDALGMYHGNIFQDAPTFPFAETKRQAGTWGVETEYENVFLCGS
                     SALRGGAVSGIPGHNAAMKVLEEIGKS"
     gene            complement(5288..>6301)
                     /locus_tag="VE486_05835"
     CDS             complement(5288..>6301)
                     /locus_tag="VE486_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HZA38641.1"
                     /translation="KLTENELTPRERKEALEVPASVTPAVRELAESWTAQNSNPRATV
                     NSALQFFRTQGFRYSLSPGEYRKNDLEQFLFQRRVGFCEHYAASFASLMRLARIPARV
                     VVGYLGGEYNDLGHFFLVRQADTHAWCEVWLPESGWTRVDPTSAVAPGRASLDLSSYL
                     ARRVASGEMETRQSTFVTQLAQSAIFTNVRLAWETLNYQWDTRLLGFDADVQDVLLAS
                     FGIANRGPFFLIIEIMVVAVALLVIYFGWMQLRTRSRVDRVRALYERFCRKLARLGVQ
                     RDPSEGPSDFSRRAAQLLPDESERIRQISNTYIALRYAPKPAAAVLDSFAKEVSAFAA
                     ASR"
BASE COUNT         1457 a         1669 c         1771 g         1404 t
ORIGIN      
        1 cacgattgcc cacaacaccg tcatcgccga agtccgctct tcatggccga aaagcaaaag
       61 cgatgtggct tcaatcacca gccatgcgat cactgcgtac acgaccgcaa ctttgtgaac
      121 gttgcgtcgc ttgagttcac caaagaaagt tttcgcatcc attgaccttg cgttgtgcca
      181 tacccaggtc ttgagagcaa gacatccgtg gggcgtccgc gttttgcctt gtatcctctg
      241 taggccgaca aaggtgttgc cgtaatcacg tgattcggga actatcgctc gagggcggtt
      301 tttcgcagcg caggcaagat gaagattctc actcttttaa tcgtatgttt cgcgtcagtc
      361 gctctggctg aggacttcaa ggcgatcgac gggaaagaat acaaaaacgt caagctcagc
      421 cgaatcgaac ccgacggcat tgtgctcatc agcaaatcgg gaatctcgaa agtttatttc
      481 agcgagttgc caaaggaggt ccaggaccgt tttcattacg atgcggcgca ggccgccgcc
      541 tactcggccg aacaaactgc cagtcaggtc gccttgcaaa atcagcaagc ggaattgcgg
      601 cggaaactgg ccgaggaaaa aaacagatat tggatcggac aagacccagc caaaaaccag
      661 aaggataagc ttccggcgtc tgctgcggtc gtcgccgacc gcggaaaaca ggtcgaagta
      721 atctcgcacg gcgcgccggt cgacatcacc aaacacctcg cgctcggcaa ggtcaccgtc
      781 gtcgattttt atgcggactg gtgcggcccg tgcaaacatc tctcaccaag cctggagcag
      841 atggcgagaa ccgatccgga gattgcgctg cgcaagatcg acatcgtcaa ttggaaaacg
      901 gcggtggtga agcaattcaa catccgctcg attccgcaag ttaacgttta caatcgcggg
      961 ggccgtctcg tcggcactgt tgtcggcagc gacgttgggg aaataaagcg ttacataacg
     1021 caagctaaaa gcggcggctg agaggccgat cgagaggtca gtggtcagcc gccttcgcca
     1081 aggctccggc gggcccaagg cagtcaatgg ttggctggct gccgtctgct cacgccttat
     1141 cgtaaacggc gacgttcttg aactttttgc ccttcggagt ggttccggta acggtgacgg
     1201 tgcggctctt gccatccgcc gcgactacga tccgcgcagt ggctgtgacc ttgccatttt
     1261 tcttggccgt aacttccaat gtccggtcat cgattttcct gtaggcgcgc gtgtcggaag
     1321 tcggatcgcc ggtgagagga tgatccgtcc cgtcaaactt gcccgaccat tcgatgtgcg
     1381 tgggcttgcc ctccccgtcg atgccgtccg acttcaccgt cacttgcccg agcatgcttg
     1441 agtaaacaac gtgtgtgttt ttcagcgtcc cgggtgcaat cttcgatttc gattcgttca
     1501 gcttccaagt gcccatctgc ggatcagcgg ctaggcatgc tgcagatccc aaaaagcaaa
     1561 acgccagagt taatccaatc gtttttgttt tcatgctcca tcgaacttgg attttcattg
     1621 ttggtcaatc tttttctaga ggtaacaggc tgcagctaag aacttgtcac tgagttagat
     1681 caggccaccc ctaatcggaa gagaggtctc ggcaagctgc cgagaccaac aggctggcag
     1741 cctgtgctcc ccagaactca tagcgtcggc aaaggttttt tcgatgttgg cggcgggaat
     1801 tcacgatcca gatcagcgaa gtcatggtcc gttaatttaa tatcagtcga gcgctcacat
     1861 ttagttatga tcatcactct ggtggtcgcg aataatcgtt acgggcgctc ctttgcggat
     1921 gagtaagtcg atttcggcaa tgtcgtcttt caacatggca attccttgag gcgggcgatc
     1981 tgcatcgcga gaatctgcga ttgtctcggc gttgtcctct tcgacagcaa tttcggaact
     2041 gctggtctca ctgtcttctt cgacgccata aagcacatag cctggtcggc ccaaatcaat
     2101 tcgtggagta ccttctttct gaagcggacg gctggaccgc accggtctgc cattttccca
     2161 aaacgatttc gccgctactt tcgtctggat cgcgggcttt cgcgatgccg gcagatcagc
     2221 cgaagcaatc gggtactgag caaaaaaccc acgaccatcg tgcacgacca cgcggtcgcg
     2281 caggagatcg atggtgatgg tgaaatcgag ctgtggcacc aggagttttt ctcccggccg
     2341 gatcaatgtg gaatcgaggt cgttcacgcg aacgatcatt tccgtactgg actcaagctt
     2401 tcgcgcgatt gatgaaagcg cgtcgccgcg cttcacggtg tattcggttt tgcgaggtga
     2461 agcttccttc gagaagaaga tttgggtgtt gacctcgccg agaaggtcgc gcaactcacg
     2521 ggtgaccggt gacttcggcg ccgtgatgag cgcgttcacc aaaattgcgc gggcttcgtc
     2581 caatttctct tcccggacca aagcctgggc ttgtttcagc ttctttacgt taggatgcac
     2641 gtaagtcacc cacggaatcg acttgtacct ctccaatcct tgagggcgaa atacgatgcg
     2701 ggcaaaatac gcgccgccac ccaagagcgc ggcgccaagc aggaggacga ggcatttttg
     2761 cgagagtgac attctcatcg ggttcgggga tcaacgcccc cttatataag gacatcgtct
     2821 gcgcgcaggc attcacgggt ttgttgccca aacccggaaa aattcaccgt atcgctgcgt
     2881 ccatcgatta ggaacgtttg atgtttgatg taagcatgaa atcgcgcgtg aactcaaagt
     2941 gaaatctcgc tacaatgtta ttggtcttcg gtgccgttat tcgggaagag ttgatagctg
     3001 acggttaagt gttgatggtc gcgggatgaa ttggtttttc cgcacgctcc tcgtgctgct
     3061 cgctattccg tcgctttcta ttggcggtca gtcagcagag caacaggcga caatcaatag
     3121 aagtgcaact tccggcgaat tattcgacac gatcgcccgc atggacagcg cgatatttga
     3181 tgcgttcaac aagcacgatg tcgatcgttt gatgtcgatg tttactgatg atcttgagtt
     3241 ttatcacgac accggtgggt tgacgaatta ccaacagagc caggaaagtt ttaagaaaat
     3301 gttcgcaagc acacccgaca tacgcagaga tctcgtcaaa ggaagcccgg agatttttcc
     3361 aatcaaagac catggcgcga tcgagatagg cgaacctcgc ttttgccata gggagaatgg
     3421 caaagacgac tgtggcacat tcaagtttgc catgatctgg cggaaaaatg gtgactcgtg
     3481 aaaaatttcg cgtgtaatca gttacgggca ccagcgatcg accaattctc acagggccgg
     3541 caagaatgaa gtccaaatat gatgtcgtca ttttaggggc aggacataac gggctcgtcg
     3601 cggcgagtta cctcgcgcgc gcggggttga gcgtactgct actggagaag aacgattaca
     3661 tcggtggggc gacgacttcg cagaaagttt tcccagatta cgaggcgcgg ctatcacggt
     3721 attcatattt ggtcagtctg ttcccggaga aaatcattcg agaccttgga ttgaatctcg
     3781 aattgcgccg gcgggcgacc gcgtcgttca cgccctacat tcaggatggc cgccataacg
     3841 gcttgctctt aagcaatgtc tccgaagaga tgagccgcca gtcggtgttc gagctcaccg
     3901 gcaattacat cgagtacgaa cagatgaaga agttttacaa tctctcgcgc gtctttgccg
     3961 agcatgtctg ggacacgatg ctggaaccgc tcgttgcgaa agaggagtta cgacgccggt
     4021 ttgatgtgga tgaggtctca cgcgaagcgt ggcgcagtct ggcggaggaa ccgctcgggc
     4081 gcgccatcga acgttatctg caaaacgatt tggtgcgcgg tctggttctt accgatggga
     4141 agatcggcgt ctcgacttat ccgcacgatc cttcgcttct gcaaaaccgc tgttttcttt
     4201 atcacctcat cgggaacaag acaggtgaat ggaaggtgcc ggctggggga atgggtgccg
     4261 tggcgcgcga gttggaacag gcggcacgta aagccggcgc cgaatcgcta acaagcgtcg
     4321 atgttcgcgc gctcgacctg aaaggagaga aaaaaacagt tgaatttgaa atcgaagccg
     4381 ccggaggcag aacgttcgcc ccgcattcgg agacggtgga agcgcgctat ctcctggtta
     4441 attttggccg caacgtgctc gcgaagttta ccggcaaatt atatcaaccg caggccgagg
     4501 acgaggggtc cgtgttcaag atcaacatgc tcctgcgccg gttgcccaaa ctaaaggccg
     4561 cgcgttacgc ggcgaccgag gctttctgcg gaacgtttca ttgtgatgaa ggctacgagc
     4621 aaatgaatgt cagctatcag caggcgacgg agggccggct cccgaacaaa attccatgcg
     4681 agctttattg ccacacgctc accgacgaca gcatcctcgc gccggaatta cgcgaaaaag
     4741 gttttcatac cctgacattg ttcggaatcg acacgccgtg gggtttgttt gcgcgcgaca
     4801 acaggacgat gagagaactc gcggagaaga aattcatcga aggtatgaat caatggctcg
     4861 aggagccgct cgaagattgc ttcgcggtcg cgcgtgatgg cagtctctgc atcgaaagca
     4921 aaagcccggt cgatatcgaa gatgcgcttg gcatgtatca cggcaacatc ttccaggacg
     4981 ccccgacatt tccctttgcc gaaacaaaaa gacaggccgg tacgtggggc gtcgaaacgg
     5041 aatatgaaaa tgtgtttctc tgtggctcga gtgcgctgcg cggcggcgcg gtcagcggta
     5101 ttcccgggca caacgcagcg atgaaggtgc tggaggaaat cggaaagagt taaataggcg
     5161 gcaaagcctc attaaattta gcaacgcgtt gaggacaaca cgttccacct ttgcgtttgt
     5221 gagggtggat tggcttgtcc tcaagacgat gctaatcgaa ccgccgaagg cgcgatatct
     5281 ttagccatca acggctagcg gcggcaaacg cactcacttc cttcgcgaag ctgtcgagga
     5341 cagcagcagc cggtttgggc gcgtagcgca gcgcaatgta cgtatttgaa atctgtcgaa
     5401 tgcgctccga ttcgtcaggc aataattgtg cggcccgcct tgagaaatcc gatgggcctt
     5461 ccgacggatc gcgttgcacg ccgaggcggg caagcttgcg gcaaaaacgt tcatataacg
     5521 ccctgacccg gtccacgcgc gagcgcgtgc gcaattgcat ccagccaaaa tagattacga
     5581 gcagtgcgac tgcgacaacc atgatttcga tgatgagaaa gaacggcccg cggttggcga
     5641 tcccgaagga ggccagcaat acgtcttgca catcggcgtc gaacccaagg agtcgagtgt
     5701 cccattggta attgagcgtc tcccaggcaa gccgaacatt ggtgaatatt gccgactgag
     5761 ccagctgcgt gacgaacgtg ctttggcgag tttccatttc tccagaagcg acgcgtctcg
     5821 ccaaatacga gctgagatcg agactcgccc gtccaggcgc aactgcgctg gttggatcca
     5881 ctcgcgtcca tccgctctcc ggcagccaga cctcgcacca cgcatgcgtg tcggcctgcc
     5941 gcacgagaaa aaagtggccc agatcgttgt actcaccacc caggtagccg acgacgacgc
     6001 gcgccggaat tcgggccagc cgcatcaaac tggcgaagct ggccgcgtaa tgctcgcaaa
     6061 agccaacgcg acgctgaaag agaaattgtt ccagatcgtt cttcctgtat tcgcctggag
     6121 acaacgaata gcgaaaacct tgcgtccgga aaaactgtaa cgcgctgttt actgtagcgc
     6181 gcggattgga attttgcgcg gtccaggatt ctgctagttc gcgcacagcc ggcgtaaccg
     6241 atgccggcac ctcgagcgct tccttacgtt cacgcggtgt gagctcgttt tctgtgagtt
     6301 t
//
LOCUS       DASQUQ010000205         6477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_588256, whole genome shotgun sequence.
ACCESSION   DASQUQ010000205 DASQUQ010000000
VERSION     DASQUQ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588256"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VE486_05840"
     CDS             complement(<1..1234)
                     /locus_tag="VE486_05840"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HZA38642.1"
                     /translation="MIRFIYNLFWPIGFLFFLPGYLLKMLRRGGYREKFGQRVGIYDR
                     DLRARLSQSRGRRPTWLHAVSVGEVMIALKLAHQLRTLEPDVPCVLTTTTTTGFALAN
                     KNAPPWIEVMYTPLDFWPVMRRAFSTIRPARIVLVEAEIWPNLAAEAHAHRIPLVLVN
                     ARLSPRSEERFRRFRFFVAPTFRLLDLICVQEQEDVDRWAALGVERSRIKYTGSIKYD
                     PAGPDQESGEFVTTSLPAAPRFDKGGQDLLPGLNPEWPVLFGGSTHRGEEEILAKIFV
                     RLRRKFPSLCLFIAPRHVERVREIRAQLEALPLQVRLASEAANHSEAQIDCVLLDTTG
                     DLRRWYEIATVVFIGKSMTAQGGQNPVEPIMARKPVVFGPHMENFVTLARTLVSKEGA
                     IQIYDVESLEKAVEKLLRD"
     gene            complement(1464..2999)
                     /locus_tag="VE486_05845"
     CDS             complement(1464..2999)
                     /locus_tag="VE486_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZA38643.1"
                     /translation="MKTLLDKLGLTDDNPGVFDGEWRGGGAKIDKISPIDGRRLASVR
                     TASDDDYKTAITRAQEAFLKWRVTPGPVRGDTVRRLGNALRELKHELGELVTLETGKI
                     IAEGEGEVQEMIDICDLAVGQSRMLYGLTIQSERPSHRLMEQWHPLGVVAVITAFNFP
                     VAVWSWNAALATVCGDATVWKPSEKTPLTAIAVTKIAERVCRETGADPAIFTLLMGDR
                     KTVGQKLADDTRLPLVSATGSVNMGLNVAKTVHGRLGRTIMELGGNNALIVAPSADID
                     MATQSIFFGAVGTAGQRCTSTRRVITHESVASKVRRKLLTAYKSLLIGNPLDRKTVMG
                     PLIDKNAVDLVHHSIGRLKDEGGEILYGGERLSVPGECYMKPCLATAKPDFEIVQHET
                     FGPLLYLMTYRDFDEAIAIHNNVPQGLTSSIFTNDVREAERFLSAVGSDCGIANVNTG
                     TSGAEIGGAFGGEKNTGGGRESGSDCWKQYMRRQTNTINFSSELPLAQGIQFGAGEGS
                     STA"
     gene            complement(3106..4494)
                     /gene="lat"
                     /locus_tag="VE486_05850"
     CDS             complement(3106..4494)
                     /gene="lat"
                     /locus_tag="VE486_05850"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR03251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HZA38644.1"
                     /translation="MSMPSVLTEAASPRSRATKSEKQKGATPSNVLDTIGRHVLLDGF
                     KVVVDLEKSRGSYLYNAVSNRRLIDLYGFFGSMPVGFNHPHFDDPDVQRDLLRAARVK
                     IANSDVYSEGYAEFVETFERVFGLPPLERYLFIEGGALAVENCLKAAMDWKVRKNMAA
                     GHGERGTQVLHFRRAFHGRSGYTMSLTNTDPRKTDLFAKFDWPRVSNPSIDFSLPESE
                     RQADVIEREAKAEREIREFIEQRKIDICAIIIEPIQGEGGDNHFRGEWLQTLRTICDE
                     NDMLLIFDEVQCGMGATGRNWCCEHFNVLPDLLAFGKKAQVCGVMAGPRIDEVKDNAF
                     RLSSRLNSTWGGNFTDMVRSTHYLRIIEEEHLVENAAKVGAYFLDQLRELQREEPMIS
                     AARGRGLFLAFDLPDPKTREEFWKGFFDLGVLTLRSGENSIRFRPALDTTADVIDEAT
                     DLMRKYCRQGTR"
     gene            complement(5335..>6477)
                     /locus_tag="VE486_05855"
     CDS             complement(5335..>6477)
                     /locus_tag="VE486_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZA38645.1"
                     /translation="AFIVDVDTPGLEITYRCRFMGLRALYNAVVKFTDVRVPRENIIS
                     KEGAGLKVALTTLNTGRLTLPAACVGLSKRLLEISRKWAGERIQWGVPIGQHSAIAGK
                     IAEMAGNVFAMEAMTFLTSSLQDRKAGDLRIETAMCKMWATETTWKIADEAMQVRGGR
                     GYETAQSLAGRGEPPIAVERFLRDCRVNTIFEGSSEIMRLFIAREALDPHLKVGGAIF
                     NTQLPVSERAKAVFSSGKFYAGWYPRQWMPNGAGKIDNLDEILRGHVDYAARTSKRLS
                     RGLFHAMARFGPKLDREQLLLSRFVGVATELFAISATCSYAQWLLGQGKPADEILSVA
                     DYFCRSARTRIDNHFAGTQRNVDRRGYQLTQELLADKHAMLRDGIV"
BASE COUNT         1388 a         1911 c         1745 g         1433 t
ORIGIN      
        1 tatcgcgcag gagtttctca actgcctttt caagtgactc cacgtcataa atctggatgg
       61 cgccctcctt tgagaccagc gttctcgcca gcgtgacgaa attctccatg tgaggaccaa
      121 acacgacagg tttgcgcgcc ataatcggct cgaccggatt ttgtccaccc tgcgcggtca
      181 tgcttttccc gataaacaca acggtcgcaa tctcgtacca gcgtcgaagg tcaccagtcg
      241 tatcgagaag cacgcaatca atttgggctt cgctgtgatt cgccgcttcg cttgccagcc
      301 ggacctgcag cggtaaggcc tcgagttgtg ctcggatttc ccggacccgt tccacatggc
      361 gcggtgcaat gaacaaacag agcgacggaa atttccgacg caatcgcaca aaaattttgg
      421 ccagaatttc ctcttcaccg cgatgagtgc tgccaccgaa gagcacaggc cactccgggt
      481 ttagaccggg caagaggtct tgcccgcctt tgtcgaacct cggcgcggca ggcagggatg
      541 tggttacaaa ctcgccgctt tcctgatcag gaccggcagg atcgtactta atgctaccgg
      601 tgtacttaat ccggctgcgc tctacgccga gggctgccca ccgatcgaca tcctcctgtt
      661 cctggacgca gatcagatcc agaagccgaa atgtcggcgc gacaaaaaat cgaaaccgtc
      721 gaaatcgttc ctcggaccgg ggcgataacc gtgcattgac cagcacgagt ggaatgcgat
      781 gcgcatgcgc ttcggcagcc agattcggcc aaatctcagc ttcaacgagg acaatcctgg
      841 ccggacgaat ggtggaaaac gcgcgccgca taaccggcca aaaatcaagt ggcgtgtaca
      901 tgacttcgat ccacggcggc gcgttcttgt tcgccaatgc gaatccggtt gtggtcgtcg
      961 tggtcaacac gcaaggcaca tcaggttcaa gcgttcgcag ctgatgggcc agcttgaggg
     1021 caatcatgac ttcgcccacg ctgaccgcgt gcagccatgt tggccttctc ccgcgactct
     1081 gggatagccg cgcgcgtaga tcgcggtcgt aaattcccac gcgttgaccg aatttttcgc
     1141 gataaccgcc gcgccggagc attttcagca aatagccggg caaaaagaac aggaacccga
     1201 tgggccaaaa caaattgtag ataaagcgaa tcacactatt ctcgaaattt gacggccatt
     1261 ctcaaagcga cgcgaggacg cggtcgcaca ggtaggcagt gcgcactccc gccttcgcaa
     1321 ggctaccgcg tggcaggccg aaagtccgcc gccggacgga ctcgtcctgt ggcgagcttt
     1381 gcgaaacaga attcacctct tcaaggattt tgcacgcagt gctttggagt gcgacgcgtc
     1441 ttcgcgtcgc tttggaacgg tagttacgct gtcgaactcc cctcacccgc tccgaattga
     1501 attccctggg cgagcgggag ctcgctggaa aaattaatcg tgttcgtttg ccggcgcatg
     1561 tactgcttcc agcaatcact gccgctttcg cggccgccgc cagtattttt ctcgccaccg
     1621 aacgcgccac cgatctcggc gccgctcgtt ccggtattca cattggcgat tccgcaatcg
     1681 ctccccaccg cgcttaagaa cctttccgct tcccggacgt cattagtgaa aatcgatgag
     1741 gttaatcctt gcgggacatt attgtgaatc gcgatcgctt catcgaaatc gcgatacgtc
     1801 atcaagtaaa gaagcgggcc gaatgtttcg tgctgcacga tttcaaaatc cggctttgcg
     1861 gttgccaggc acggcttcat gtaacactca cccggaacgc tgagtcgctc cccgccgtag
     1921 aggatttcgc cgccttcatc tttcaatcgg ccgatggaat gatgcacaag atcgaccgcg
     1981 ttcttatcga tgagcgggcc catcacagtc ttgcgatcga gcggattccc aatcagcaaa
     2041 gatttgtatg cagtcaaaag tttgcgtcgg accttggatg ccaccgattc atgggtgatc
     2101 acgcgtcgcg tggaagtgca gcgttgcccg gccgtaccta cggcgccgaa aaagatcgat
     2161 tgcgtcgcca tatcgatgtc ggcgctgggc gcaacgatca gcgcattgtt cccgcccagt
     2221 tccatgatcg tcctgcccag ccggccatga accgttttcg cgacattcaa gcccatgttt
     2281 accgagccag tggccgagac cagtggaagc cgcgtgtcgt cggccagttt ttggccgacc
     2341 gttttgcgat cccccatgag cagtgtaaaa attgccggat cagcgccggt ttcgcggcaa
     2401 acgcgctcgg cgatttttgt gaccgcaatt gctgtgagcg gcgtcttttc cgatggcttc
     2461 caaaccgtag catcgccaca aaccgtcgcc agcgcagcat tccacgacca gactgcgacc
     2521 ggaaaattaa acgcggtaat caccgcgacg acgccgagcg gatgccattg ctccattaag
     2581 cgatgacttg gccgttccga ttggatggtc aggccgtaga gcattcgcga ttgaccgacc
     2641 gcgagatcgc agatgtcgat catctcctgc acttcgcctt cgccttcggc gatgattttc
     2701 ccggtctcga gggtgacgag ctcaccgagc tcatgcttca attcgcgcag cgcgttacca
     2761 agtcttctca ctgtgtcgcc gcgaaccggc cccggtgtta cgcgccactt taaaaacgct
     2821 tcctgcgcgc gcgtgatcgc ggttttgtag tcatcgtcag aagccgtgcg gacactggct
     2881 aatcggcgcc catcgatcgg cgagatttta tcgatctttg caccgccgcc gcgccattcc
     2941 ccatcgaaga cgccagggtt atcgtcggtc aatccgagtt tatcgaggag agttttcata
     3001 ggtggatcga ccgctccgcg gacgatggca aaataatggc ggcgaagccg cgtagattta
     3061 acatcgagca agggactgct cgatccaccc ttttcgcact actcattacc gtgtcccttg
     3121 acggcagtat ttacgcatca agtctgtcgc ttcgtcgata acgtccgctg tggtatcgag
     3181 tgcggggcga aaacgaatcg agttttcacc ggaacgaagc gtcagcaccc caagatcgaa
     3241 aaagcctttc caaaattcct cgcgcgtttt tggatcggga agatcgaacg ccagaaacag
     3301 tcccctgccg cgcgcggcgg aaatcattgg ctcttcgcgt tgcaactcgc ggagctgatc
     3361 cagaaaatat gcgcccactt tcgcggcgtt ctcgaccaga tgttcctcct cgatgatgcg
     3421 caggtaatga gtggagcgca ccatatcggt aaagtttccg ccccaggtgg aattcaaccg
     3481 gctggataag cgaaacgcgt tgtcttttac ttcatcgata cgcgggccag ccatcacgcc
     3541 gcagacctgc gcttttttcc caaacgcgag cagatcaggc aacacattga aatgctcgca
     3601 gcaccaattg cgacccgtgg cccccatgcc gcattgcact tcatcaaaaa tgaggagcat
     3661 gtcgttttcg tcgcaaatcg ttcgcaacgt ttgcagccat tcgccgcgga aatgattatc
     3721 gccgccttcc ccctggatcg gctcgatgat gatggcgcag atatcgatct tgcgctgctc
     3781 aatgaactcg cgaatctcgc gctccgcttt tgcttcgcgc tcgatcacat cggcctggcg
     3841 ctccgattcc ggcaacgaaa aatcgatcga cgggttagac acgcggggcc aatcgaattt
     3901 ggcgaacaaa tccgtcttgc gcggatcagt gttcgtaagg ctcatggtgt aaccactgcg
     3961 accatgaaat gcgcgacgga aatgcaaaac ctgggtgccg cgttcgccat gacctgcggc
     4021 catgttcttg cgcactttcc agtccatcgc ggccttcaaa caattttcga ccgcaagcgc
     4081 gccgccttcg atgaatagat aacgctccag tggcggcaaa ccgaacacgc gttcaaacgt
     4141 ctcgacaaat tccgcgtagc cctccgagta aacatccgag ttcgcgattt ttaccctggc
     4201 cgcccgcaga agatcgcgct gaacatcggg atcatcgaag tgtggatgat taaacccgac
     4261 cggcattgag ccgaaaaatc cgtagagatc gatcaatcgc cgattgctaa ctgcattata
     4321 taaataggaa ccgcggctct tttcgaggtc gacaacgacc ttgaaaccat cgagcaaaac
     4381 atgtcgcccg attgtatcga gcacattcga cggcgttgct cctttttgtt tttcgctctt
     4441 tgtggcgcgg cttcgaggtg aagccgcttc cgtgagcacg gacggcatac tcatccgttg
     4501 atcgtaatct ggagcgggaa cgcggtcaat ggctccgccg acgacaacgg ccgataacgc
     4561 accggcccgg gccgaggcgg tggagtcgga tgcggcggcg cagtcgcccc cggcgtggga
     4621 cgcggcggta atgttggcgt cggcgtcggc ccgagctccg ttgttctcag aattgcacca
     4681 tatcctccga ccgctgttcc cgtattcgca tcggtaaagg agacaccgtg aggtcacggt
     4741 ttgtgccgct gggttgacgc atccaattct gccctccatc tgttgttttc aggattacgc
     4801 ccagtgcgcc gacagatgtt gcggtctgcg catctgtgca agaaagtcct aaaaggactt
     4861 caggcacgcc gctggtctga ctaacccacg tttgccctcc atcggttgtt ctgagaatcg
     4921 tgccttgatc gccaacgacc gtcccggtat tggcatcacg aaagcaaaca gcccgaagtt
     4981 ggctagttgt tccgcttgct tgcggaaccc agttctgccc tccgtcggtt gtccgtaaaa
     5041 ttaaaccatt gttaccaacg acggccccgt tattggcatc gggattacat ctgcgtgagc
     5101 cagagagctg atgagaagtg cggttgatgc gaaccatgaa agaagtctga aggttttcat
     5161 aggtgaatcg tttggtccgc gatcctatcc caatcgcttt ggtgcagtcg atctaaattt
     5221 gattgcgttc ctaccgcgct gaacgcgatc aaattgggag cgcattgcgc ggtggatctg
     5281 ggtagcgcac gcgagacgcg tgcgctaccc aatcatgagg gcttccgcgc gcgattatac
     5341 aattccgtcg cgcaacatcg cgtgcttatc ggccagcaac tcttgggtga gctgatatcc
     5401 gcgcctgtcc acattgcgct gcgtgccggc gaagtgatta tcgatccgcg tgcgcgcgga
     5461 gcggcaaaaa taatctgcga cggaaagaat ttcgtcagcc ggtttgccct gacccagcaa
     5521 ccattgcgcg taagaacagg tcgcgctgat agcaaacagt tcggtagcga ccccgacaaa
     5581 tctggaaagg agcagttgct cccgatcaag tttcgggccg aaccgtgcca tggcatgaaa
     5641 gagaccgcgc gagagccgtt tgcttgttcg cgccgcgtaa tcgacatggc cgcgcaatat
     5701 ttcgtccaaa ttgtcgatct tgcctgcgcc gtttggcatc cactggcgcg gataccatcc
     5761 ggcataaaat tttccggaac taaaaacggc ctttgcgcgc tcggacacag gcaactgcgt
     5821 attgaagatt gcgccgccaa ctttcaaatg cgggtcgagc gcctcgcgcg cgatgaagag
     5881 ccgcataatt tcgctcgatc cttcgaagat tgtattgacg cgacaatcgc gcaggaatct
     5941 ttccaccgcg atgggcggct cgcctctgcc ggcgagcgat tgcgccgttt cataacctcg
     6001 gccgccccgc acttgcatgg cctcatcggc aattttccag gtcgtttcgg ttgcccacat
     6061 tttgcacatc gcggtttcga tgcgcagatc gccggccttt cgatcttgca gcgaagaagt
     6121 caaaaacgtc atcgcttcca tggcgaaaac gttgccagcc atctcggcga tttttccggc
     6181 gatggcggaa tgttgaccga tgggcacgcc ccattgaatg cgttcccccg cccatttccg
     6241 cgagatttcg agcagccgtt tggaaagacc gacgcacgca gcgggaagcg tgagccgccc
     6301 ggtgttcaac gtggtcagcg cgacttttaa ccctgcgcct tccttcgaga tgatgttttc
     6361 gcgaggcacg cgcacgtcgg tgaatttcac caccgcattg taaagcgcgc gcaatcccat
     6421 gaaccggcag cgatacgtga tctccagtcc tggtgtatcg acatcgacaa tgaacgc
//
LOCUS       DASQUQ010000206         5223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_520396, whole genome shotgun sequence.
ACCESSION   DASQUQ010000206 DASQUQ010000000
VERSION     DASQUQ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520396"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VE486_05860"
     CDS             complement(<1..467)
                     /locus_tag="VE486_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38646.1"
                     /translation="MPATIEHNRTTAVSPRSVFVLRFISTVALWSVALLIAFSGFEIL
                     FWLLISAFGMIGLLEFYRMLDHKGLPNFKITGMICGAVMLGGSFYYFSKIGPAGSYDF
                     EVAVLLFFLLTVFARQMFARLRQDEPLQTMAYTLFGLLYVLWLFNFVTKIVYLT"
     gene            567..1610
                     /gene="tdh"
                     /locus_tag="VE486_05865"
     CDS             567..1610
                     /gene="tdh"
                     /locus_tag="VE486_05865"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HZA38647.1"
                     /translation="MQKTMQAVVKSRAEPGLWLEEVPVPEVRGDDVLIRVLKTSICGT
                     DVHIYNWDAWAQKTIPVPMTIGHEFVGVVDDVGDHVKGFEKGDLVVGEGHITCGHCRN
                     CLAGRRHLCPNTQGIGVNRPGAWAEFVAIPQDNCWHADASISLDVLSCFDPLGNAVHT
                     ALSFDLVGEDVLITGAGPIGCMAIPICKRAGARNVVMTDINPYRLDLARKMGVDLALD
                     VRTEKLVDAMEKLGMKEGFDVGLEMSGSPKAFIDILPNMFHGGKIALLGIMPGSAAID
                     WNVVVFNQLTIKGIYGREMYETWYKMTAMIQSGLDISPVITHRFHYKQFKEAIELMKS
                     GNSGKIVLDWGER"
     gene            1758..2504
                     /locus_tag="VE486_05870"
     CDS             1758..2504
                     /locus_tag="VE486_05870"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38648.1"
                     /translation="MNTRIDQIVGGIYRISTWQEPYGITFNQFLIDDERPTLIHTGMR
                     DLHDVIRKTIREVLDPAKLAHVILLHWEGDENGGMNQFMKEAPRSELIGSLLSIQLNA
                     MGFGLADRTRGFRDGETLDLGKHKLRFLETPHVHHWDSMMVFDETTRSLFPSDLFIQP
                     ADQPPVVTENLSMPMIDLYRAAGIFAHELPIRQVVDRVEKLNLAWIHPMHGGSFEQSA
                     FSNYAKALRENEFAYRGMLLGREIAKAAPM"
     gene            complement(2509..3264)
                     /locus_tag="VE486_05875"
     CDS             complement(2509..3264)
                     /locus_tag="VE486_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38649.1"
                     /translation="MTYAERRNLALAVLAAFIAIILLSACGTTERALPPYEPPLTKTD
                     FQNVRTTAYTHTESDHREYSNRNALGGELRAAGPPIHRAEAAPRALPAYDVPRAIPVD
                     ETVETEVRRVSRSPQLQPFSMSERRTTRTVKTRKGRSTSSAQARMVPGSAAADWSRWP
                     AGTVFRLLSTGQTYRVEDYGWALSGRNTIDLYMANKREMNSWGARGESIQILQWGDPE
                     ESLQFLRSHQNYKHIKRMVLELEGRHKDAAALR"
     gene            complement(3863..4255)
                     /locus_tag="VE486_05880"
     CDS             complement(3863..4255)
                     /locus_tag="VE486_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZA38650.1"
                     /translation="MRTRATLPKSQPCEATFKRLLWNLRKRGLTGGDLLEIGCGYGYL
                     LDEGRLMFQRRVGTEFSSEGAAIARATGAEVFVGGIEQVPPGMEFDCITATQVIEHVY
                     EPLSFVQRLANRTKPGGHPHRCATDTNW"
BASE COUNT         1186 a         1429 c         1458 g         1150 t
ORIGIN      
        1 gtcagataaa cgatcttggt gacgaaatta aaaagccaaa gcacataaag caggccaaac
       61 aaggtatagg ccatcgtttg caacggttcg tcctggcgca gacgcgcaaa catctggcgc
      121 gcgaaaacgg tcagtagaaa aaagagcaac accgcaactt cgaagtcgta ggaaccggcg
      181 ggcccaatct tggagaaata ataaaaactg ccgccgagca tgacggcgcc gcaaatcatt
      241 cctgtgatct tgaaattcgg cagacctttg tgatcgagca tccgataaaa ttccaggagc
      301 ccgatcattc cgaacgcact gatcaacagc caaaataaga tttcgaaccc ggagaacgcg
      361 atgaggagcg cgacagacca gagggcgacc gtgctgatga atcgcaaaac gaaaacggag
      421 cgcggcgaaa ccgcagtcgt cctgttatgc tcaatggtcg ccggcatgca tttgcatcat
      481 ccccaaggat gacgcgccgc gcaaggagaa cggcgcagga tcgataacgc acattgcgcg
      541 gcaaatcgtc cggcttactt tgcgtcgtgc aaaagacgat gcaggccgtg gtcaaaagcc
      601 gggccgagcc gggattgtgg ctcgaagaag ttccggttcc tgaagttcgc ggcgacgatg
      661 tgctcatccg cgtcctgaag acctccatct gcggaaccga cgtccacatt tacaattggg
      721 atgcatgggc gcagaagacc attcctgtgc cgatgacaat tggccatgag tttgtcggcg
      781 tcgtcgatga tgtgggtgat cacgtgaaag gatttgagaa gggcgatctc gtcgtgggcg
      841 aggggcatat cacttgtgga cattgccgga attgccttgc cgggcggcgt cacctttgtc
      901 cgaacacgca aggcatcggc gtaaatcggc ccggagcatg ggcggagttt gtagcaattc
      961 cacaagacaa ctgctggcac gccgatgcgt cgatctcgct cgacgtgctt tcgtgtttcg
     1021 atccgcttgg caacgctgtc cacaccgccc tctcgttcga tctcgtcggt gaagatgtcc
     1081 tgatcaccgg cgctggaccg attggttgca tggccattcc gatctgcaaa cgagccggag
     1141 cgcgtaacgt cgtgatgacc gatatcaatc cctaccgtct cgatcttgcg cgcaagatgg
     1201 gggtcgatct cgccttggat gtgcgcaccg aaaaacttgt cgatgccatg gagaagctcg
     1261 ggatgaaaga aggctttgac gtcgggcttg aaatgtccgg cagcccgaag gctttcatcg
     1321 acatcctgcc gaacatgttt cacggcggaa aaatcgcatt gctcggcatc atgcccggca
     1381 gcgcggcgat cgattggaac gttgtggtat tcaatcagct caccatcaaa ggcatctatg
     1441 gccgggaaat gtacgaaacc tggtacaaga tgaccgccat gatccagtcc gggctcgaca
     1501 tctcaccggt gatcacccat cgcttccatt acaagcaatt caaggaagcc attgagctga
     1561 tgaaatccgg taactccggc aaaatcgtgc tcgattgggg cgaacgctga atttcaaccg
     1621 cgaatcacgc ggatcacgca gatattccgg gggcgcaggc gccctcgtct gctcttttcg
     1681 gcgccctcgc cgaaaaagcc tctcgcgcta tgaacagttt ttggtacggt tttcccatcg
     1741 tatgaaggag ttgcgttatg aatacacgca ttgaccagat cgttggcggg atttatcgga
     1801 tttcgacctg gcaggagccg tacggcatca catttaatca gtttttgatc gatgatgagc
     1861 ggcccacgct gattcatacc ggcatgcgtg atctacacga cgtcatccgg aaaaccattc
     1921 gcgaggtgct ggatcccgcg aagttagcac acgtgattct gttgcattgg gaaggcgacg
     1981 agaacggcgg catgaatcaa ttcatgaagg aagcgccgag atccgagctt atcgggtcgc
     2041 tcttatcaat tcagctcaac gccatgggct ttgggctcgc tgatcgaacg cgcggttttc
     2101 gcgatgggga gacgctcgat ctcggcaagc acaagctgcg ttttctcgaa acgccccatg
     2161 ttcaccactg ggattcgatg atggttttcg atgagacgac gcgcagtctg ttcccgtcgg
     2221 atttgttcat tcagccagcc gaccaaccgc ccgtagttac tgaaaattta tcgatgccga
     2281 tgattgactt gtatcgcgca gctggaattt tcgcgcacga gttgccgatt cggcaggtcg
     2341 tcgaccgcgt ggagaaactc aatctcgcgt ggattcaccc gatgcacggc ggctcatttg
     2401 agcagagcgc attttcaaat tacgcaaagg cattgcgtga aaacgaattt gcctatcggg
     2461 ggatgttact gggtcgggaa atcgcgaaag ccgccccgat gtgatgcgtt accgcaacgc
     2521 tgccgcatcc ttatggcggc cttccagctc gagcaccatt cgtttgatgt gcttgtagtt
     2581 ctggtgcgat cggaggaatt gcaagctttc ctccggatcg ccccattgca gaatttggat
     2641 gctctccccg cgcgcgcccc atgaattcat ctcccgttta ttcgccatgt aaaggtcaat
     2701 ggtattgcgt ccggagagcg cccagccgta gtcctccacg cgataggttt gtccggtaga
     2761 aagcaagcgg aagactgtgc ctgcgggcca gcgcgaccaa tccgccgccg cactcccggg
     2821 aaccatccgc gcctgagcgg acgatgtcga tcttcccttt ctggtcttga cggttcgcgt
     2881 tgttcgtctc tccgacatcg agaatggttg caactgcggc gaacgggaaa cgcgccgcac
     2941 ttctgtctcg acggtctcat ctaccggaat agcgcgcgga acgtcatacg ccgggagcgc
     3001 acgcggcgcc gcttccgccc ggtgaatggg cggacccgct gcgcgcaatt cgcccccgag
     3061 cgcgttacga ttactatatt cgcgatgatc cgattcggtg tgcgtatatg ctgttgtccg
     3121 cacattttga aaatccgttt tcgtcagtgg cggctcatac ggcggcagcg cgcgctcggt
     3181 ggttccgcag gcactgagca aaatgatggc tatgaatgcc gcgaggacgg ctaaggcaag
     3241 attacggcgc tcagcgtagg tcaaggcgaa tcaccagcgc gagttattaa cagctattca
     3301 caattctgac aagactattt ttcagaagat tttgacctga caaaggcgct aaaacccaaa
     3361 agaatggcaa agaaggaaat cgtcgaattt gtggcggagg atgatccagg cagcaattcg
     3421 tttaggcaac aggcttcttg cgcgcgcatt caatttggcc acaatgccac aacgctttcc
     3481 tttccatgat caccgccgtg gaattcgaaa gatggtaccg ccaccattcc tcatctttta
     3541 tgcgtgcgac gaggctgcgc ggattgtaaa cgtgttacat ttttggcacg gtgcgcggcg
     3601 atcaccggaa tttcgctgag cgggctcgaa catgaacaag tgcctgattt tttgcgccgt
     3661 gttgttatag cagcctcgct gcgtaacacg cgcaaagttg tgcgaaggcg ggtgcggcgc
     3721 ggtggggcga cgagggtttg cgtatggacg ctttaggagt tgagtaggtt tccactagcg
     3781 actatcaacc gcctgctacg atgttggctc tcatttatct gggattggcg atttgcgtcg
     3841 gcgatcagct ctgccgtcgc ttttaccagt tcgtatccgt tgcacaccgg tgggggtgac
     3901 caccgggttt cgtacgattg gctaatcgct gaacgaacga caatggttcg taaacgtgct
     3961 cgattacctg cgtcgcggtg atgcaatcaa attccatccc aggcggaacc tgttcgatac
     4021 ccccgacaaa gacttcagcg cccgtcgcgc gtgcgatcgc cgcgccctcg gacgaaaatt
     4081 ccgtcccgac gcggcgctga aacatcaacc gcccctcgtc cagtaagtag ccgtagccgc
     4141 aaccgatctc caaaagatcg ccgccggtca atccgcgctt ccggagattc cacagcagcc
     4201 gtttgaatgt cgcttcgcaa ggctgactct tgggcaatgt agctcgtgtc cgcataaccg
     4261 caggtgccgc cctcgtaata tgacgactgg cgatacacct gctgcatggc actctccatc
     4321 agccgagggt aaagatagta caacccacat gccatgcagc gcgccactcc gtacggatcg
     4381 cacaaacgaa acggaaactg acgctggtcg ttcttgcata acgggcaggt tgggtattca
     4441 aggttcgtag acacgtttgc ggcggtttcc tcccgattgt ttttttattc gaaactgagc
     4501 aagcggcgag ttctttcacg gattctctct ggatctctgc ttagctggat tagcgcatgc
     4561 acaatgactc cgcaggcttc aaccgaataa aataaaccat atccactcga gccgatgaca
     4621 agcagtcgca ccggctcttc aaacattgga gcgatctcgg agtgtgtgcg caggttctca
     4681 agggtgaagt cgagtttgcg aacgaaccgg acgcccgatc cttcgctaag atcttccagg
     4741 tcagcaaaat ttggagattg ccctcagcgc ctcctttcca aataatttca ggcatgccgc
     4801 cgcttcaaga gcgggtcgcg caactcttcc caagacacac cgcctactgg gttcgccttg
     4861 tggtcatcga ggcggcggcg cagcagaccc gccacaggcg ggctggaaac tgagaacagc
     4921 gccaagagga caccggcgag gacggggcag agcaatcaaa gcgcggcgac taacaaaggc
     4981 ggatcgggaa gttgatcttg ttttcatgag tggaatgcga gctttgcgct aacggcgagc
     5041 gccgttttag tcagaagcgc ggcagcctgt caagcattcc gcgatttttg gaccgacgga
     5101 tgaaaccgag cctccttcgc gaaccttcga caggatcgat cgctgccttt tcgccaaagc
     5161 gttccgtctt agcatgacga tgtgaaaccg gaagtcagcg catcatttcg tttggatcat
     5221 acg
//
LOCUS       DASQUQ010000207         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_47102, whole genome shotgun sequence.
ACCESSION   DASQUQ010000207 DASQUQ010000000
VERSION     DASQUQ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47102"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VE486_05885"
     CDS             <1..960
                     /locus_tag="VE486_05885"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HZA38651.1"
                     /translation="HFKGSKVTTVGDDIAWMQVRDGRVYAVNPENGYFGVVPGTSYKS
                     NPNAMKSIGHATLYTNVALMDDGDVWWEGKDGAPAHAIDWRGKDWTPDSKEKAAHPNS
                     RFATPMRNNPALDPDVEKGEGVPISAIIFGGRRSDTMPLVFQAFDWEHGVYLGATMAS
                     ETTAAATGAVGQVRRDPMAMLPFCGYNIGDYFRHWLEIGKRLTNPPLIFNVNWFRKGT
                     DGKFLWPGFGENMRVLKWIIDRCEGTGEAVKSPIGWLPGPHDLDLEELDVEHKYVIEI
                     LGIDHEDWQKEFAEHKKFFDSLGGVVPEELVRQREQLAARFSV"
     gene            1184..>3363
                     /locus_tag="VE486_05890"
     CDS             1184..>3363
                     /locus_tag="VE486_05890"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38652.1"
                     /translation="MGSKIAKKERVIAKDFRTDCVEIRGARQNNLKDIDVDLPLGKLT
                     VVTGPSGSGKSSLAFDTIYAEGQRRYVETFSPYMRQFLDRMDKPRVDDIRGIPPAIAI
                     EQSNPVKSSRSTVGTMTEINDYLKLLWPRVAQAFCPGCGREIRPETAKSIADQIICHF
                     ERSRAATQPPEAARPGFQIPLRNPSVTSRDPSTSLRSARDDGKTVLVTFWVAVPAKTE
                     PREFFDFLQQQGYLRVWIDNEIVRVDAEPKIKRLGARVQVIQDRIAISEENRPRLVEA
                     IETALRFGKGQVNIIPISRNAPRLTDHRSLITDHQIPFSTGWHCAHCDLDIRPPSPGL
                     FSFNNPLGACPECRGFGRTIALDLNKAIPDRSLSIKQGVVRAFRGAEFGESQKDLLRA
                     CAREEIDINVPFEELPKTDQDFVIEGEKRSGDYTDEDYENDRWYGVRGFFRWLERKTY
                     KMHVRVLLSRYRAYTTCPSCNGGRFQPEALNYKISATPNPRSEILLTLPEFAALPISD
                     ARDFLRKIAIAPTDSTAGMLRNEICARLNYLCEVGVGYLTLDRSTRTLSGGEVQRVNL
                     TTCLGASLVNTLFVMDEPSVGLHQRDVGRLVRVMHNLRDKGNTLLVVEHEEQIIRAAD
                     NLIDIGPGRGERGGELAWSGGLDDFLAGDNANLDSARGPRAGDGVPPSRTFRSASSRS
                     GGKPRKSLFRRDAEINARDARATQSLTRDYLSGRKSIPVPKARR"
BASE COUNT          756 a          946 c          975 g          686 t
ORIGIN      
        1 catttcaagg gctcgaaagt cacaaccgtc ggggacgaca ttgcctggat gcaggttcgc
       61 gacggccggg tttatgccgt caatccggag aacggctact tcggcgttgt gccgggcact
      121 agttacaaat cgaatccaaa cgcgatgaag tcgatcgggc acgccacgct ctacacaaat
      181 gtcgcgctca tggatgacgg cgatgtctgg tgggaaggca aggacggtgc gcctgcgcac
      241 gcaatcgatt ggcgcggaaa ggattggacg ccggattcaa aagagaaagc cgcgcatccg
      301 aacagccggt tcgccacgcc gatgcgcaac aatccagcgc tcgatcccga tgttgaaaaa
      361 ggcgaaggcg tgccaatcag cgcgatcatt ttcggcggac gccggagcga cacgatgccg
      421 ctcgtgtttc aggcgttcga ttgggaacac ggcgtttatc tcggcgcgac catggcttcg
      481 gaaacaaccg ccgccgcgac cggggcggtc ggacaagtgc gacgtgatcc aatggcgatg
      541 ttgccgtttt gcggatataa catcggcgat tacttccggc attggctcga gatcgggaaa
      601 cgtctcacca atccgccgct catttttaat gtgaactggt ttcgcaaagg cacggacgga
      661 aaatttctct ggcctggttt cggtgagaac atgcgggtgc tcaaatggat catcgatcgc
      721 tgcgaaggaa ctggggaggc ggttaaatcg cccattggtt ggttgcctgg gccgcacgat
      781 ctcgatctgg aagagctcga tgtggagcac aaatacgtca tcgaaattct cggcatcgat
      841 catgaggact ggcaaaaaga atttgccgag cacaaaaagt ttttcgattc cctcggaggc
      901 gttgttcctg aggagttggt gaggcagcgc gagcaactcg cggcgcggtt cagcgtgtaa
      961 ccgccgaccg gccacggaat tccggggagc gcacgcggcc cgtgtgctgt tttcggccgc
     1021 tggccgaaaa cctcccacgg tgccaacaaa cgcatcgtac agaataaccc atcgcgaatt
     1081 ttgcaatgcc tctgaagccg tttggccagc ggccaaacgg agcacgccag cggcgtgcgc
     1141 tcccaaaatt tgcgaaccgc gccgcaatct ggaatcgtct ccggtgggct cgaagattgc
     1201 gaaaaaagaa cgcgtcatcg cgaaagattt taggacggac tgcgtcgaga ttcgtggtgc
     1261 gcgtcaaaac aatctgaagg acatcgatgt cgatcttcca ctcggtaaac tgacggttgt
     1321 tacggggccg agcggcagcg gcaaatccag cctggctttc gacacaattt acgcagaagg
     1381 ccagcggcga tacgtcgaaa ccttcagccc ctacatgcgg caattcctcg atcgcatgga
     1441 caagccgcgg gtggatgaca ttcgtggcat tcccccagcg atcgccatcg aacaatcgaa
     1501 cccggtaaag tcgtcgcgtt cgaccgtcgg cacgatgacg gaaatcaatg attatttgaa
     1561 attgttatgg ccgcgggtcg cgcaggcgtt ttgcccgggc tgcggccggg aaatccgtcc
     1621 tgagacggcg aaatcgattg cggaccagat catctgtcat ttcgagcgaa gtcgagctgc
     1681 aacgcagccg ccggaggcgg cgaggccagg ctttcaaatc ccgttgcgaa atccgtcagt
     1741 gacttcacgg gatccctcga cttcacttcg ttccgctcgg gatgacggga aaactgtcct
     1801 cgtcacattc tgggtagctg tccccgcaaa aaccgaaccg cgcgaatttt tcgattttct
     1861 tcagcagcaa ggctatctgc gcgtgtggat cgacaatgaa attgtgcggg tggatgcgga
     1921 accaaaaatc aaacggctcg gcgcgcgcgt acaggttatt caagaccgca tcgcgatcag
     1981 cgaagaaaac cggccgcgcc tggtcgaagc catcgaaaca gctctccggt tcggcaaagg
     2041 ccaggtaaac atcatcccaa tttcgagaaa cgccccacgc ctcactgatc accgatcact
     2101 aatcaccgat caccaaatcc ccttctccac cggctggcat tgcgcgcatt gcgatctcga
     2161 cattcgtccg ccaagtcccg ggctattcag ctttaacaat ccactcggtg cgtgtcccga
     2221 atgccgcgga tttggccgca ccatcgcgct cgatcttaac aaagcgattc cggatcgcag
     2281 tttgagtatc aagcaaggcg tggtgcgcgc ttttcgcggg gctgagtttg gcgaatcgca
     2341 aaaggattta ctgcgggcct gcgcccgcga agaaatcgat atcaatgtgc ctttcgagga
     2401 actgccgaag accgatcagg atttcgtgat cgaaggcgag aagcgctcag gtgattatac
     2461 cgacgaggat tacgaaaacg atcgctggta cggcgttcgc ggttttttcc gctggctcga
     2521 gcgcaagact tacaagatgc atgtgcgcgt tttgctcagt cgctatcgtg cttacacgac
     2581 ctgcccgagt tgcaacggcg gacgctttca gcccgaggcg ctcaattaca aaatttccgc
     2641 aacccctaat ccgcgatccg aaattctgct gactcttcct gagttcgctg cgctccccat
     2701 ttccgacgcg cgcgattttc tacgcaagat tgccattgcc cctaccgact cgacggcggg
     2761 aatgttgcgc aacgagatct gcgcgcgact caattatctt tgcgaggtcg gcgttggtta
     2821 tcttacgctg gatcgttcga cgcgcacatt aagcggcgga gaagtgcagc gggtcaatct
     2881 gacgacctgt ctgggagcgt cgctcgtgaa cacgcttttt gtcatggatg aaccgagcgt
     2941 tggtttgcat caacgcgatg tcggcaggct ggtgcgtgtg atgcacaacc tacgcgataa
     3001 aggcaatacg ctgctcgtgg tcgaacacga ggaacaaatc atccgcgcgg ccgataactt
     3061 gatcgacatt ggccccgggc gcggcgagcg cggcggcgaa ttggcctgga gcggcggcct
     3121 cgacgatttt ctcgcgggcg ataacgccaa tttggatagc gcacgcggcc cgcgtgctgg
     3181 cgatggcgtt ccgccatcgc gaacttttcg aagcgcatca tcacgatcgg gtggaaaacc
     3241 cagaaaaagc ttgtttcggc gcgacgccga aatcaacgcg cgagacgcgc gtgctaccca
     3301 atccttaacg cgtgattatc tcagcgggcg caaatcgatt ccggtaccga aagcgcgccg
     3361 gaa
//
LOCUS       DASQUQ010000208         9333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_588729, whole genome shotgun sequence.
ACCESSION   DASQUQ010000208 DASQUQ010000000
VERSION     DASQUQ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588729"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VE486_05895"
     CDS             complement(<1..216)
                     /locus_tag="VE486_05895"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZA38653.1"
                     /translation="MTIFEKIIARQIPAEIIWEDDDAIAFHDVNPQAPVHALIVPKRV
                     VPRLADATENDRALLGKLLLVARDLAKK"
     gene            complement(337..897)
                     /locus_tag="VE486_05900"
     CDS             complement(337..897)
                     /locus_tag="VE486_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38654.1"
                     /translation="MVAFKEEFPYLRTDSGQLFEFNRDWLHAAITRAADEAGYPSWWL
                     TDHVTESIAFYLRLRTNENVVAFSQLSQTVRYVLKVIGYKEIVPHFAPSPPPISISLL
                     DIAHEAGTGYELAFFDLLEKRINMLVETGVDDLQLCSLQSCVKHLRGVKAWTRACDAL
                     REEIVCFVREKLMASTDLARLDCSLR"
     gene            complement(884..1441)
                     /gene="nrdR"
                     /locus_tag="VE486_05905"
     CDS             complement(884..1441)
                     /gene="nrdR"
                     /locus_tag="VE486_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZA38655.1"
                     /translation="MRCPKCGSRDDKVIDSRQSRDSSSIRRRRECLKCKYRFTTYEEI
                     ERSDLRVVKRDRTHEPFDRRKLAGSIAKAFEKRSTSLLILEDMVDEIVHELETGGREV
                     PSSAIGAKVLEKLRAIDEVAYLRYASVYQRFQDVDQFVDAIHTLGRRVKTNALQRELF
                     PPETTPGRSAAGSRTIAVNKNHGRV"
     gene            1605..2591
                     /locus_tag="VE486_05910"
     CDS             1605..2591
                     /locus_tag="VE486_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZA38656.1"
                     /translation="MTRRTADATRFGMAERRSSGPTYGPWLVGLGAALWGTESAWRIP
                     LNALFDAKVIVFWEHVIILIMFLPLLVPRLGEIPKIQARTWGYLLFSAFAGSAVGTIF
                     FTLALKYGNPTVVNVILNIQPVISTIGAFLVFGDRLAPRFFLYASVAILAGIFVSVAH
                     PALIGVSFERAGLSQGTGYALICAFFWGLATVAGRGVMIGMSLRLASSLRIVVGLICM
                     TLILLAYGKLGGAALWPAAAQAHATKAIVLLVLLASVSGGIPLLIYFEGLRLTRASTA
                     GYFEMMQTLAAVCITWGFFHASLYPHQVVAALILIGAVAMVHHVQRQVEGHD"
     gene            2789..6601
                     /locus_tag="VE486_05915"
     CDS             2789..6601
                     /locus_tag="VE486_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZA38657.1"
                     /translation="MALAVFLALLAIFHRPILLAIGRQLVLRYAARENLRADFRLEGN
                     PFSNLTVRNLHAFAAGPSGIESIDIGYLYLDYSLFGFARHGLSHLLDNVEARSARIVL
                     NPSKAPLKPRPPKPRLELPKLFPERIRLTDATLVVRNQPNDFVAEHVDLDLNPRSQGE
                     LRVELLQLTAGDRWSKISAPVSYANKNLIVREVVLSDQEKIQLLNVDASRIDANTLAT
                     NLNATVGGGQLSASATFKETKSSLDTKIHLAAEKVAAESLNKFLILPSGPLSGEVEHL
                     AIDGTGVIDSPRSWNGTMSLAMNNVQQPGINFNRGVIEVSAEQGKATLRSADIVQDKN
                     EFHLRGTIDLPATFKDFGRTPTTLEVTGTAADLQQLTAGTPLRLTGSAQFTGKIGIVN
                     AKIEANLGVTASSVGFPEGTMEKMSATLRVSKVVPPADSKKPWFADLRTAMEIGLTNI
                     HYRDYVVDSVNGSLSGLDDVLGLDRVSLRRNQNELNVRGRYRLPADPGKASSQPADLE
                     VAFNAPETGDFWAVDSPNRMSGPLQMTAQLEWKQEVANGNISISGSDLKMRDLVFRQL
                     SVQSSVSNNVIYLKDCRAILNDTDFINAAGSLNLRKPYHYNGRISARVADLSTLQPLL
                     HASSNENELAGSFALDWEGNGNAQTVKNSGKLKLVLGEGRYGNLQSVQANIDASYSPE
                     GLDVPIIFFATNNMDFQAIAQAKGETLEISKIQLDQSQAKFASGYISIPFVWGNLGTS
                     APVVPSSGKVTATFQSENLDIRRVFEDIGVKPMASGILNAKLDAKGTIADLNARLDVQ
                     MRDLRSEGWPHLEPATFDLTAEVVHNQLAIVGKLQQTHIQPLELTANMPFDVPKIARA
                     RKLPDETPITAKARLPRSSVNFVRQFVPDLEQLDGEVGLDVDVSGTIGNPVLRGAGDM
                     RITTARFTNATLPALHNLNARLNFAGDTITLEGFEGSLAGGPFKMSGRVTFPRLTEPT
                     LDLQLKAESMLVARNDSLTARADADLKVTGPFLTATVAGNVAITNSHFLKNIDLIPIG
                     LPGRPAPQPPSARPEFSLPDPPFRNWKFDVAVKTKDPILIRGNLATGSAVSDLKLTGT
                     GLHPELAGLVRMENVEATLPFSRLQVSNGFLYFDPSDSFNPRIDLQGTSVIRDYTVRV
                     YVYGTMLAPQAIFTSEPPLAQEEIISLIATGATRAELSGGNVLAGRAAMLLVQQLYRK
                     IFKKGEPTQSNTAFNRLELELGTVDPRTGRQQASARFRISDRFVIVGDVGVGGNYRGK
                     LKYLIRFR"
     gene            6611..8782
                     /locus_tag="VE486_05920"
     CDS             6611..8782
                     /locus_tag="VE486_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZA38658.1"
                     /translation="MQHRILFCLASVALTLKVAAQSAVDVTRPEEKQKARQAVEKKQE
                     KRAQQTSIIEFRGQQAFDEKALRSALKEEITTIDDFGLSPARADDLAFFLEVFYRKHG
                     YAKVDVHYAIESENRLRLDITEGPRFTLGTVIFDGNAREPNDKLLEYVLGPTRERYSK
                     LEKNLPFVAADIQEGAGLVHRLYVAEGFLDSDVDPPRYTFHDQTSQVDVIIPIHEGQQ
                     YFFGSISFTGQKIYDNETLRGQMLDLLRQPFTDVRVADIPRRLQAYFKARGYYDVQVS
                     VAAAPEQAVNGRVPVQVIIHAGSVYRFDGITVTGLERLRPSYVIKRFSKLQGKTYSPD
                     ALDERFRTLMRTGLFNIVQIKPVPVDGHLLRLDISAEEAKSKEFGFSIGYGTYEGGIA
                     GVQYRDRDLFGYGRPLTTSIEVSQRGYRGEILWEDPFFFDTDFAFKARLAAFTFRFDG
                     YTKFELGGRLELTRKLTKHDEAGVTFAVRHVKIIEADIKQPFLGDENYLVNTLGLTNT
                     LDMRESPYVTPRGFLINNTLDLASSAFGSDIEFIRGTMRVGYYLPFGPKPLTPGLVED
                     KPGTPFQRWFQQSAIAFGARAGIIHSLTVSGPAEATAIPIDERFFNGGGDTVRSFAER
                     DLGPHDRKGHPIGGEFFTVFNIEYTFPILGELQGALFTDAGNLLPTSEEPGLDDMRYA
                     VGVGLRYKLPVGPIRLDYGVNPDPHEDEDFGAFHFSFGFAF"
     gene            complement(8971..>9333)
                     /locus_tag="VE486_05925"
     CDS             complement(8971..>9333)
                     /locus_tag="VE486_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZA38659.1"
                     /translation="STDLNERHPFDVEILRIPPGKAPYSYHSHSAQWEFYHVISGSGV
                     VRHKDGTSTIEAGDAFIFKPGEPHQITNDTTQDMILYVVADNPIGDTYYQPDSNKWGC
                     ADTGAQTGSFGRAGLLRR"
BASE COUNT         2086 a         2579 c         2565 g         2103 t
ORIGIN      
        1 cttcttagcg agatcgcgtg ccacaagaag caatttcccg agtaacgcgc gatcgttttc
       61 ggtcgcgtcc gcgaggcgcg gcaccactcg tttgggcacg atcaacgcat gcacaggcgc
      121 ctgcggattg acatcgtgga aagcaattgc gtcgtcgtct tcccagatga tttccgccgg
      181 gatttgtcgt gcaatgattt tttcaaaaat cgtcatggca tgaagtaatc agtcacctaa
      241 tgaattcgac tacatctgcg caatctggtt gtaattcaac tgcgaaaatt gcaacctgcc
      301 tcgcacattt gtccaccttc ccactgcgct aaccggttag cgcagtgaac aatcgagccg
      361 cgcaaggtct gttgacgcca ttaatttttc gcgcacgaag cagacaattt cctcgcggag
      421 ggcatcgcag gcgcgcgtcc atgctttcac gccgcgaaga tgtttcacgc aagattgcag
      481 cgagcaaagc tgcagatcgt ccacgccggt ttcgacaagc atgttgatcc gcttttccaa
      541 aagatcaaaa aacgcgagct cgtaacctgt tcccgcctca tgagcaatgt ccagcaaaga
      601 gatggaaatc ggcggaggcg agggcgcaaa atgcggaacg atttccttgt aaccgatcac
      661 tttcaggacg tagcgcacgg tctgcgatag ctggctgaag gccaccacat tttcgttagt
      721 ccgcaggcgc agataaaacg cgatgctctc ggtcacgtga tcggtcagcc accagctcgg
      781 atagccggct tcatcggccg cccgcgtaat cgcggcgtgc agccaatcgc gattgaactc
      841 gaacagttgc cccgaatctg tgcggagata tggaaattct tccttaaacg cgaccatggt
      901 tcttgttaac tgcgatggtg cgcgatccag ccgcggacct gccgggggtt gtctccggcg
      961 ggaataattc acgctggagc gcattggttt ttacccggcg gccaagcgta tgaatggcat
     1021 cgacaaattg gtcaacatcc tgaaagcgct ggtacacgga agcgtaacgc aaataggcca
     1081 cttcatcgat cgcgcgcagt ttctcgagca ctttggcgcc aatcgccgac gaagggactt
     1141 cgcgcccgcc tgtttcaagc tcgtgcacaa tttcatccac catatcctcg aggatgagaa
     1201 ggctggtcga acgtttttca aaagctttcg cgatgcttcc ggccagtttg cgccgatcaa
     1261 acggctcatg tgttcggtca cgtttcacga cgcgcagatc cgatcgctcg atttcctcat
     1321 atgtagtaaa gcgatatttg catttcaggc attcgcgacg gcgtcggatg ctcgaactat
     1381 cccgcgactg acgggaatcg atgaccttgt catcacgaga cccgcacttg gggcaacgca
     1441 tagtattacg gcgctaaata gcataccatt tgttgtggcg tcaatgcttc tttgatcttt
     1501 cttttgcttt tcaatcgaaa ccagcgtttg aattgccaca ttcacgggat cgggaattag
     1561 atcggtgagt aattcgcttg cggattggcg atgaaccatc cgcattgacg cgacgcaccg
     1621 ccgacgctac acgcttcggg atggcggagc gcagatcgag cggcccgacg tacggtccgt
     1681 ggcttgttgg tcttggcgcc gcactctggg gcactgaaag cgcatggcgc attccgctca
     1741 atgcgctgtt tgacgcaaaa gtgatcgtct tttgggaaca cgtaatcatc ctgattatgt
     1801 tcctgccgct tctagtgccg cgcttgggcg agattcccaa aatccaagcg cgcacctggg
     1861 gatacctgct cttctccgcc ttcgccggct cggctgtcgg caccatcttt ttcactctcg
     1921 cgctgaaata cggaaatccg acggtcgtga acgttatctt gaatattcag ccggtaattt
     1981 caacgatcgg ggcattccta gtcttcgggg accggcttgc cccgcgcttc tttctttatg
     2041 ccagcgttgc gattcttgcc ggcatcttcg tttcggttgc gcatccagcc ctgatcggtg
     2101 tctcgtttga gcgcgccggg ctgagccaag gtaccggtta cgcactcatc tgcgcctttt
     2161 tctggggcct cgcgaccgtg gctgggcgcg gcgtcatgat cggcatgtcg cttcggctcg
     2221 cctcaagtct gcgcatcgtc gtcgggctca tttgcatgac gctgatcctt ctcgcctacg
     2281 gcaagcttgg cggtgcggcg ctctggccgg cagccgcgca agcgcatgca accaaggcga
     2341 tcgttttact ggtcctactt gcttcggtct caggaggaat tccgctcctg atttacttcg
     2401 aagggctacg cctcactcgc gcatcgacgg cgggctattt cgagatgatg cagacgctgg
     2461 ccgcagtttg catcacctgg ggcttctttc acgcgagtct ctacccgcac caggtcgttg
     2521 cggcgctcat cctcatcgga gcggtggcta tggtgcacca cgtccaacga caggttgaag
     2581 gacacgactg atcgcgggcg gcttatccac tactgggatc ataaaggtgg cgctacggtt
     2641 tcacagcgga gaaccgagca aatcacaaag cgcaatcgcg ccatttactc tttgttagtg
     2701 tggaaataga ctcgctgcag tggccgagga ccgtcaggca gagacgaaga tatacaggcg
     2761 ccggtggtcc gtaagccgcc ggacggcggt ggcgcttgcc gtattcctgg cacttctcgc
     2821 aatttttcat cgtccaattc tgctggcaat cggccggcag cttgtgcttc gttacgccgc
     2881 cagagaaaat ctccgggcag attttcgcct cgaaggtaat ccctttagca acttaaccgt
     2941 gcggaatctt cacgcatttg cggccggccc ttcgggaatc gaatcgattg atatcgggta
     3001 tctttatctt gattacagcc tgtttggatt cgcgcggcac ggtctctccc atttgctgga
     3061 caatgtagaa gcgcgttcgg cgcgcattgt tcttaatccg tccaaagcgc cgctgaaacc
     3121 gcgtccgccg aaaccacgac tagagctccc gaaacttttt ccggagcgaa tccggctgac
     3181 ggatgcaacg ctggtggtca gaaatcaacc gaacgacttt gtggccgagc atgtcgatct
     3241 tgatctgaat ccgcgtagtc agggagagct gcgggtcgag ttgttgcaac tgacggcggg
     3301 ggatcgctgg tccaaaattt ccgcgccagt ttcgtatgcg aataagaacc tgatcgttcg
     3361 cgaagtcgtc ctaagcgatc aggaaaaaat tcagcttctc aatgtcgatg cttcgcgcat
     3421 cgatgcgaac actctcgcga ccaatctcaa tgctacggtc ggcggtggcc aactgtccgc
     3481 gtcagcgacc ttcaaagaga caaaatcttc gctggacacg aagatccatt tggcagctga
     3541 aaaagttgcg gcggaatcgc tgaacaaatt tctgattttg ccgtccggac ctctctcggg
     3601 agaagtcgag catctcgcta ttgatggaac aggcgtgatc gattctccac gcagctggaa
     3661 tggaacgatg tcgctggcga tgaacaacgt tcagcagcca gggatcaatt tcaatcgggg
     3721 cgttattgaa gtttcagcgg aacaaggcaa agcgactttg cgatcggcag acatcgtcca
     3781 ggacaagaac gaatttcatc ttcgcgggac catcgacttg cccgccacat tcaaagactt
     3841 tggccgcact ccgacaactc tggaagtgac tggcacggcg gcggacctcc agcagctgac
     3901 tgcgggcacg ccactccgat tgaccggctc ggctcaattc accggaaaga tcggcatcgt
     3961 caacgcgaag atcgaagcaa atcttggtgt gaccgcgagt tcggtcgggt ttcccgaggg
     4021 aaccatggaa aagatgagcg ccacgcttcg cgtgtccaag gtcgtgccgc ctgcggacag
     4081 taaaaagccg tggtttgcag atcttcgcac ggcgatggaa atcggtttaa ccaatatcca
     4141 ttaccgcgat tacgtagttg actcagtcaa cggatcgctc agcgggcttg atgatgtgct
     4201 cggcctggac cgggtaagtc tgcgccgaaa tcaaaacgaa ctaaatgtcc gcgggcgata
     4261 ccgccttcca gcagacccgg gcaaagcttc ctcacaaccg gcggacctgg aggttgcctt
     4321 taatgcgcct gaaaccggag atttttgggc cgtggattcg ccaaacagaa tgagcgggcc
     4381 attgcaaatg acagcgcagc tcgaatggaa acaagaggtc gccaacggaa acatctcgat
     4441 ctccggatcg gacctcaaaa tgcgggacct ggtttttcgg caactgagcg tgcaatcgtc
     4501 ggtttccaac aatgtgatct acctgaagga ttgccgcgcg attctgaacg acaccgattt
     4561 cattaacgcc gccgggagtc tcaacttgcg aaagccctat cattacaatg gcaggatttc
     4621 cgcgcgggtc gcggatttat cgacactgca accattactt cacgcttcca gcaacgagaa
     4681 tgaactcgcg ggctcatttg cgctcgattg ggagggaaat ggcaacgcgc aaacagttaa
     4741 gaattccggg aagctgaagc tcgttctcgg ggaaggccgt tacggcaatc tgcaatcggt
     4801 tcaggcaaat atcgatgcgt cctactcgcc cgagggtctc gatgtcccca ttatcttttt
     4861 cgcgaccaac aacatggatt ttcaggcaat cgcccaggca aagggcgaaa cgctggagat
     4921 cagcaagatt caactcgatc agagccaggc aaaatttgcg tcaggctaca tctcgattcc
     4981 gttcgtgtgg ggaaatctcg ggacgagcgc gccagttgtg ccgtccagcg gaaaagtcac
     5041 cgcgacgttt caatctgaga atctcgacat acgtagagta ttcgaggaca tcggcgtcaa
     5101 accaatggca tcaggaattt tgaatgcgaa gctcgatgcg aaagggacga tcgctgacct
     5161 gaacgcgcgg ctcgatgtgc aaatgcgcga tctgcgaagc gagggatggc cgcatctcga
     5221 gcccgcgacg ttcgatctca ccgcggaggt cgtccacaat caactggcga tcgtcgggaa
     5281 gctccaacaa actcatattc agccgttgga actcactgcg aacatgccgt ttgatgttcc
     5341 aaaaattgct cgcgcccgaa aactgcccga tgaaaccccg atcacggcca aagctcgtct
     5401 cccgcgcagc tcggtgaatt tcgtccgtca gtttgtccca gatctcgagc aattggacgg
     5461 ggaagttggc ctggatgtcg atgtaagcgg aacaatcggc aatccggttt tgcgcggagc
     5521 gggggacatg cgaatcacca ctgctcgatt cacaaacgcg actctccccg cgcttcataa
     5581 cttgaatgcc cgactaaact tcgccggcga taccatcacg ctggaaggct tcgagggaag
     5641 cctcgccggc ggcccgttta agatgagtgg acgagttacg ttcccgaggc tgaccgaacc
     5701 gactcttgat ctgcaattga aagctgagag catgctcgtt gcccgaaacg attcgctcac
     5761 tgcgcgcgca gacgccgacc tcaaggtgac gggaccattc ctcaccgcca cggtcgccgg
     5821 caacgtcgcg ataacgaaca gccatttctt gaagaatatc gatctcattc cgatcgggct
     5881 gccgggcaga cccgccccgc aaccgccgtc ggcgcgcccg gaattttccc tgccggaccc
     5941 gccattccgg aattggaaat ttgatgtcgc agttaaaaca aaggacccca ttctcattcg
     6001 cggaaatctg gccactggca gtgcagtctc cgatctcaaa ttgaccggca ctgggctgca
     6061 tccggagttg gcgggtttgg tgcgaatgga gaatgtcgaa gccacattgc cattcagccg
     6121 tctccaagtt tccaatggct ttctctattt tgatccgagc gactccttca atccgcggat
     6181 cgatctgcag ggtacgtctg tcatccgcga ttacaccgtg cgcgtttatg tttacggaac
     6241 catgctggca ccgcaggcca tttttaccag tgaaccgcca ctggcacagg aagaaatcat
     6301 ttctttgatc gccacaggcg ccaccagagc ggagttgtcg ggaggcaatg ttctggccgg
     6361 gcgcgccgcc atgttgctgg tgcagcaact ttaccggaaa attttcaaaa aaggcgagcc
     6421 gactcagagt aacacagctt ttaatcgact ggagttagaa ctgggcacgg tcgatcctcg
     6481 aactggtcgg cagcaggcca gtgcgcgctt cagaatcagc gatcgatttg ttatcgttgg
     6541 cgatgtcgga gtcgggggca actatcgcgg gaaattaaag tatctgattc gttttcgcta
     6601 gacccctaag atgcagcaca ggattctttt ctgcctggca agcgtggcgc taacattgaa
     6661 ggtcgcggcg cagagcgcgg tggatgttac gcggcccgaa gaaaagcaaa aggctcgcca
     6721 ggcggtcgag aaaaagcagg aaaagcgcgc gcaacagacg agcatcatcg aatttcgtgg
     6781 ccaacaggct ttcgatgaaa aggcgctgcg ttcggctttg aaagaagaga tcacgacaat
     6841 cgatgatttt gggttaagtc cggcacgggc cgacgatctg gcgttcttcc tcgaagtttt
     6901 ttaccgaaag catggctacg cgaaggtgga cgtgcattac gccattgaat cggagaatcg
     6961 cctccgtctc gatattaccg aaggcccgcg cttcacactt gggactgtaa tctttgatgg
     7021 aaatgcgcgg gagccaaacg acaagctctt ggaatacgtg ctcgggccga cgcgggagcg
     7081 atattcaaag cttgagaaaa atttgccctt cgttgctgct gacatccagg aaggtgcggg
     7141 tctggtgcac cgtttgtatg tcgcggaagg gtttctggat tctgacgtcg atccgccccg
     7201 ttacactttc cacgatcaaa ccagccaggt tgatgtcatc attccgattc acgaggggca
     7261 acaatatttc ttcgggagca tttcatttac cggacagaaa atttacgata acgaaacact
     7321 gcgcggccag atgctcgatc tgctgcggca gcccttcacc gatgtacgag tggcagatat
     7381 tccgcggcgt ctccaggcat atttcaaggc gcgcggctat tacgacgttc aagtctcagt
     7441 tgccgccgcg cccgagcagg cagtgaatgg ccgggtaccg gtgcaagtca tcatccacgc
     7501 aggttctgtt taccgctttg acggcattac ggtaaccggg ctcgagcggc tgcgtcccag
     7561 ttatgtcatc aaacggttca gtaaactcca gggcaaaact tacagcccgg acgccctcga
     7621 tgagcgattt cgcaccctga tgcggacggg gctctttaac atcgtccaga tcaagcccgt
     7681 tccagtcgat ggacatctct tgcggcttga catctcggca gaggaagcca agagcaaaga
     7741 attcggtttt tcgatcggat acggcacgta tgaaggcggg atcgccgggg tgcaatatcg
     7801 agatcgcgac cttttcggtt acggcagacc actgaccaca tcgattgagg tctcacagcg
     7861 gggatacaga ggggagattt tgtgggagga tccgtttttc tttgatacgg attttgcctt
     7921 caaagctcgt cttgctgcgt tcactttcag attcgatggc tacacaaaat ttgaattggg
     7981 cggacgactc gagcttacgc gaaaactcac aaagcacgat gaggccggcg tgaccttcgc
     8041 tgttcggcac gtcaaaataa tcgaggctga catcaagcaa cccttcctcg gcgacgagaa
     8101 ctatctggtc aatacgctcg gcttaacgaa cacgcttgat atgcgggaaa gcccttatgt
     8161 cacgccgcgg ggattcctga tcaacaacac attggatttg gcttcgagcg ccttcggaag
     8221 cgacatcgaa tttatccgcg gaacgatgcg ggtgggctat taccttcctt ttggaccaaa
     8281 accgcttacc ccgggcctgg tcgaggataa accgggcact ccgtttcagc gttggttcca
     8341 acaatctgcg atcgcattcg gcgctcgtgc cggcattatt cactccctta cggtgagcgg
     8401 tccggcggaa gcaaccgcga ttcccatcga cgaacgtttc ttcaatggcg gcggcgacac
     8461 cgtccgtagt tttgccgagc gcgatcttgg cccgcacgac cgaaaaggtc accccatcgg
     8521 cggcgaattt ttcacggtat ttaacatcga atatacgttt ccgattttgg gcgaactaca
     8581 gggcgcgctt ttcacggatg cgggcaatct gctgccaacg tcggaggaac caggcctcga
     8641 cgacatgcgc tacgcggtag gcgtagggtt gcgctacaaa ctaccggtcg gccccatccg
     8701 cctcgattac ggcgtcaatc cggatccaca tgaagacgaa gattttggcg cattccattt
     8761 cagcttcgga ttcgcttttt aggcatgcgc tcgcgccaca tttgctagct gtagccgaac
     8821 gacgatgctc aagacctaac gaaacggaga tgagcgaccg ccgccgagag cgcgcgtgga
     8881 tcgcagtgaa tgggttctga tcacgagaaa cgaagatgca cagcgggcgg cggttcgctc
     8941 catcgattgg ttagacgacg gtctcattcc tcaccgtcgt aataatccag cgcgtccgaa
     9001 cgaaccagtc tgcgctccgg tatcggcaca cccccactta ttgctgtcgg gttgatagta
     9061 tgtatcgcca atcggattgt cggccacgac gtaaaggatc atgtcttggg tggtgtcatt
     9121 ggtgatctgg tgaggctcac ccggcttgaa aatgaatgcg tcgccagcct caatcgtact
     9181 ggtcccatct ttgtggcgga cgacgccgct gcctgaaatc acatggtaga actcccactg
     9241 tgcgctgtgc gaatgatacg agtagggagc cttccccggc ggaatacgca aaatctcgac
     9301 atcaaaggga tgccgctcat ttaggtctgt gga
//
LOCUS       DASQUQ010000209         3873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_708338, whole genome shotgun sequence.
ACCESSION   DASQUQ010000209 DASQUQ010000000
VERSION     DASQUQ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708338"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..804)
                     /gene="rplA"
                     /locus_tag="VE486_05930"
     CDS             complement(103..804)
                     /gene="rplA"
                     /locus_tag="VE486_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZA38660.1"
                     /translation="MQRNRSKRYRAAIEQVDRTKAYNLPEAVSTLKKMPATKFDQTVT
                     VSFRLGVDPKQSDQMVRGTCPLPHGSGKQVRVLVFAEGEAAKAAKEAGAEFVGMKDLI
                     QQCQEGFQDFDVAIATPAAMAEVRKLGKVLGPRGLMPNPRTGTVTDDTAKAVQEVKAG
                     RVEFKLDKNGNVAVPVGKFSFEEQALVENGNAVIEAVVRARPATAKGRYLEGMTISAT
                     MSPGVRVDPAPYLSV"
     gene            complement(920..1885)
                     /locus_tag="VE486_05935"
     CDS             complement(920..1885)
                     /locus_tag="VE486_05935"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HZA38661.1"
                     /translation="MAFVPACLFGAPQVSSLAYLLVEKGYTPLTMKRAGNEFYVSCKL
                     NGRSASLIVDTGAGRTVIAAPVLRSLGVPLTKGEENVYGVMGLAGKSKAGEINDVQIG
                     PYQAGAHPVWAWDFSSWGRTAGGPGMDGLLGIDFLHRHQAVIDCFRMHLFLKSPSAAS
                     VSNTLRAGLKAGGCTEIPIQATRQGLTVPTRINGRAGYLILDTGAALTTLSQHAIVGL
                     NMRLVSATRLNVRLGAQDVGRNVSVINKAHFKTMEVGNFSIPPQWVGVADLPHAKSGE
                     TDNVFFGYLGHDLLACYVGIIDCAALKLYLRFDPVIDAARRKRGG"
     gene            complement(2033..3790)
                     /locus_tag="VE486_05940"
     CDS             complement(2033..3790)
                     /locus_tag="VE486_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZA38662.1"
                     /translation="MSSPASQSLINLLLESGVPSSEEAAQLAVNANGGSWVGQVLDSG
                     KVDEQRFLGAIGNFFRVPVVSIHSKKIDRAALSILPSRFVFQHHILPIEVKEDSVVLA
                     TYDLFNSIGRQLASQLLKKPAEWVLVPRSQLLRAMKTLYGIGAETFDEILKTNRSFEA
                     LQDTETAIELNADDPEASVVKFVNQIIREAIIERATDIHVEPLENDLRIRYRIDGILH
                     EVAVPPQLRVLQSAIISRLKVMAHMDIAERRLPQDGRMNLQANNQNIDVRVSTIPTVN
                     GESISLRLLSRSEQQFGFDRLDLSKKQETIIRNLLAQPNGIILLTGPTGCGKSTSLYC
                     FLSSINSVQRRIITIEEPVEYRLPGVSQIDVKPEIDLTFAKGLRHILRQDPNVVMVGE
                     IRDVETADIAIRAAMTGHLVFSTLHTNDAVGGITRLLDMDVEPFLLASVVKAFIAQRL
                     VRTICPDCVELVDYPHEYLAEIGVPVKELGTRFQRGTGCDQCRQTGYQGRAAIYEICL
                     VTEPLRKLIMRKCDGGELKQCAIAEGMETLRQDGWRRVAQGKTTIEEVVRVTQTDEVM
                     AETTEEAEPVMAAESVGVH"
BASE COUNT          837 a         1119 c         1029 g          888 t
ORIGIN      
        1 acaagcacaa aaggcgagcg tttcaatttc tcggaaagat ctgagacgat gctggttttt
       61 tctggtcgca tattagcggt ctaattattg tcgatctaaa gctcagacac tcaaatacgg
      121 cgctggatcg acgcggactc ccggagacat cgtcgcacta attgtcatgc cttcaagata
      181 acgtcctttc gccgtcgccg ggcgcgcccg gacaaccgct tcgatcactg catttccatt
      241 ttcaaccagc gcctgttcct caaaggaaaa ttttccaacc ggcactgcga cattgccgtt
      301 cttgtcgagc ttgaattcaa cgcggccggc cttcacttcc tggacggcct ttgccgtgtc
      361 gtcggttacc gtcccggtcc tgggattggg cattagtccg cgcggtccga gcacttttcc
      421 gagcttccga acttccgcca ttgccgcggg cgtggcgata gcgacatcaa aatcttgaaa
      481 accttcctgg cattgttgaa tcaggtcttt catcccgaca aattctgcgc ctgcttcttt
      541 agccgccttt gccgcctcgc cctcggcgaa aacgagcacg cgcacctgtt taccgcttcc
      601 atgaggtagc gggcaggttc cgcgcaccat ctggtcggat tgctttgggt caacccccaa
      661 ccggaacgaa accgtcacgg tctgatcgaa cttcgtggcg ggcatttttt tcagcgtcga
      721 gacggcctca ggcagattgt aagccttcgt cctatccacc tgctcgatgg cggcgcgata
      781 gcgtttgctt cgatttcttt gcatggcgtt ttcagttcta acgagccgtg aagctctcct
      841 gattcatgtt ccgcgcagct attggagcaa cggggagcgc ataaactagc gggatcgacc
      901 ggcctgtcaa ccggatcgcc tatccgcctc tttttcttcg cgcggcgtcg atgaccggat
      961 cgaatcgcag gtaaagcttc agcgcagcgc aatcgatgat cccaacataa caggcgagca
     1021 ggtcgtgacc taagtagcca aaaaatacgt tgtctgtttc gccgctcttc gcgtgaggca
     1081 agtccgccac accgacccat tgcggaggga tgctaaagtt gcctacctcc atcgtcttaa
     1141 aatgagcctt gttaatgacg ctcacatttc tgccaacgtc ttgcgctcca agccggacgt
     1201 tgagacgggt tgccgaaact agacgcatgt tgagtccgac aatcgcatgt tggctgagcg
     1261 tagtgagagc cgcgcccgta tctaggatca ggtagccggc acgtccgttt atccgggtgg
     1321 gaacggtcaa tccctggcgc gtagcctgga ttggaatttc tgtgcagccc ccagccttta
     1381 gacccgcgcg cagcgtgttt gaaacagatg ccgctgatgg agacttcaga aagagatgca
     1441 ttcgaaaaca atcaatcacc gcttggtggc ggtggagaaa atcgattccc aagaggccat
     1501 ccatacctgg accgccagcg gtgcgacccc aggacgaaaa atcccaagcc cacacgggat
     1561 gtgctcccgc ttggtagggg ccaatctgaa catcattgat ttcgccagcc ttgctcttgc
     1621 cagccaaccc catcacacca tacacatttt cttccccttt tgtgagtggc acaccgagcg
     1681 atcgcaatac tggcgccgcg atgacggtcc tgcccgctcc tgtgtcgacg ataagactgg
     1741 cggagcgccc attcagtttg caggagacat aaaattcgtt acctgccctc ttcattgtca
     1801 atggcgtata acccttttcc accagcagat aagcgagcga agagacttgg ggagcgccaa
     1861 acaaacaggc aggcacaaag gccattccag cggccactgc aaatgagatc aggttttttc
     1921 gcatccgacg ctactgtcta agtcagcagg aagtctcctg gcaagccgat aacgtttgcc
     1981 cccgaaagcc ttcagggctt gggatgaccg ctccaacagg attgggcgat ctttagtgta
     2041 cgcctaccga ttcggcggcc attaccggct cggcttcctc ggtcgtttcg gccatcactt
     2101 cgtcggtttg cgtaacgcgc acgacttctt cgatggtcgt tttgccctgg gcgacacgcc
     2161 gccagccatc ctgccgcaag gtttccatgc cctcggcgat ggcgcattgc ttgagctcgc
     2221 cgccatcgca ctttcgcatg atcagctttc tcaagggttc agttacgagg caaatctcat
     2281 aaatcgccgc acgtccctgg tagccggttt gccggcattg atcgcaaccg gtgccgcgct
     2341 ggaaacgagt gcccagttct ttcacaggaa caccaatctc ggccaggtat tcatgcgggt
     2401 aatcgaccag ctccacgcaa tctgggcaaa ttgtgcgcac gagccgttgc gcgatgaacg
     2461 ctttcaccac cgatgcgagc aggaacggct ccacgtccat gtcaagcaag cgggtgatgc
     2521 cgccgacggc atcgttggtg tggagcgtgc tgaaaacaag gtgaccggtc atcgccgcgc
     2581 ggatcgcaat atccgcggtt tcgacgtcgc gaatctcgcc caccatcacc acgttgggat
     2641 cctgtcgcaa aatgtgacgg agacctttcg caaaggtcag gtcgatttcc ggtttcacat
     2701 cgatctgcga aacacccggc aatcggtatt ccaccggctc ttcgatagta atgatgcgac
     2761 gctgcacgga attgatgctg ctgagaaagc aataaagcga ggtcgatttt ccgcagccgg
     2821 tcgggccggt gagaagaatg attccgttag gttgtgcgag cagattgcga atgatagtct
     2881 cctgtttctt gctcaaatcg agacggtcga atccaaactg ttgttcgctg cggctgagca
     2941 gtcgcaaact gatcgattcg ccgttgacgg tgggaatggt ggacacgcgc acgtcaatat
     3001 tttgattatt cgcctgcaaa ttcatgcgcc catcctgagg caaacgacgc tccgcgatgt
     3061 ccatgtgcgc catcaccttc agtcgtgaaa tgatggccga ttgcaatacg cgcaattgcg
     3121 gcggaacggc cacttcgtgc agaatcccgt cgattcgata ccggatgcgc agatcgtttt
     3181 ccagcggctc aacgtgaata tcggtcgcgc gctcgataat tgcctcgcga atgatctggt
     3241 tcacaaattt caccaccgag gcttcgggat cgtcggcatt cagctcgatg gcggtctccg
     3301 tatcctgaag agcttcgaag gaacggttcg ttttcaggat ttcgtcgaac gtttccgcgc
     3361 cgatgccgta gagcgttttc attgcccgca aaagctgtga ccgcggcact agcacccact
     3421 cggccggttt cttcaaaagc tgggatgcga gctggcggcc aatcgaattg aaaagatcgt
     3481 aggtcgccag caccactgaa tcctctttca cctcgatcgg gaggatgtga tgttgaaaaa
     3541 caaaacggct cggcaggatc gacaatgcag cgcgatcgat cttcttggaa tgaatcgaga
     3601 caactggcac acgaaagaaa ttcccgatcg cgccgaggaa gcgttgctcg tccaccttgc
     3661 ccgaatccag gacctggcct acccaggaac cgccgtttgc gttcacggct agttgtgccg
     3721 cttcctcgct cgaagggacg ccgctctcga gcagcaaatt aatcaacgat tggctggctg
     3781 gtgaactcat taggcgcggc gttcagtaga atcgacaatg cagaatcgga agcgttcgaa
     3841 tgaacccgat ttacgccgct tcgcaagggc ttt
//
LOCUS       DASQUQ010000210         7025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_227555, whole genome shotgun sequence.
ACCESSION   DASQUQ010000210 DASQUQ010000000
VERSION     DASQUQ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227555"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..463)
                     /locus_tag="VE486_05945"
     CDS             complement(86..463)
                     /locus_tag="VE486_05945"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZA38663.1"
                     /translation="MKDDIENQQTREMKQESPTIGICPGITRRTIANGKTMYQMVATL
                     AAGSRMPAHSHPQEQIVHILEGQMRLIVDDVPHELSTGDSFYLASNVPHGVETLLPTR
                     VLDTFSPPRDDYLAIDRERMASD"
     gene            complement(835..1470)
                     /locus_tag="VE486_05950"
     CDS             complement(835..1470)
                     /locus_tag="VE486_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38664.1"
                     /translation="MKRLTNVIDSYPQFSKDGKLLLFQSNRSGKTQIYVSKADGTEIR
                     QLTYLDFPAAVPCWSPDMSRIVFAGEPAGHSEIFVMNADGSGIVQLTRTNGDDSHPHW
                     SANGSRIMFNSSRMTPDPAADWSKQWHELFSMKPDGSGVLQHTRLRTVCTYGSFSPDM
                     KHIVYRKVIEGPAFQWDLSAIGRNSEVFVANADGSNEVNLSNNAAFDGWPV"
     gene            complement(2304..3773)
                     /locus_tag="VE486_05955"
     CDS             complement(2304..3773)
                     /locus_tag="VE486_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZA38665.1"
                     /translation="MRGLRCVCSILLTTAGAVLFVLDTGGCAKHRLPGGLSKLKPCRL
                     PGISEELFCGKLTVFENRETRTGRTIDLNIVVLPAFDQKTKAGPVFDLAGGPGASSAD
                     RADFYAGPGKVYRRRHDVVCVDQRGTGKSNRLAIPREKTPSYYLSEMFPIDHVKEMRH
                     TLEQRADLTKYTTSIAMDDLDEVRSWLDYDRISLFGASYGTRAALVYMRQHPEHVRSA
                     ILLGVAPTDLKMPLHHAESGARAMDLLLGECEQDARCHAAFPQIRDDWKNVLAQLEKQ
                     PARVEYSPRQNAAPTTVEIQRGVFGEKIRTWMYDRDKAARIPLIVHRAANGDLAPFLQ
                     QAIGPSIPDFVADGMYLSVTCAEDVPFINPEEAAKLTAGNPFGNYRVFQQTRACGMWP
                     RGEIPTDFLDPVSSNAPVLIFSGNMDPVTPPRYGEEVARHLPNSRHVIIPQAGHSVDG
                     LSDAGCIDRIAIEFLEKGDAKNLDVSCVERMAPPPFVTK"
     gene            3940..4497
                     /locus_tag="VE486_05960"
     CDS             3940..4497
                     /locus_tag="VE486_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38666.1"
                     /translation="MNTTAILLLVIIVAVIAVGAFLFWQWRRSAALQQRFGPEYERAV
                     GEFGDQRKAEAELAAREKRVRTLDIRSLTPEEQARFTEAWKKAQARFVDEPSQAAADA
                     DILVKKLMETRGYPVGDFEQRAADISVAHPAVVSNYRAAREIALRNKTGKATTEDIRQ
                     AMVHYRSLFEELLETAEPATRKEEG"
     gene            4500..4910
                     /locus_tag="VE486_05965"
     CDS             4500..4910
                     /locus_tag="VE486_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38667.1"
                     /translation="MESKTDQPTMEIAGDQRRSDQPSSTEEVARDLENRKGTTGKPAE
                     IEKPTPLFPENEATNFRNRWTEIQAAFVDEPRRAVEQADALVADVIKRLASSFADERS
                     KLEGQWGRGDNVSTEDLRVALQRYRAFFDRLLSV"
     gene            4938..5762
                     /locus_tag="VE486_05970"
     CDS             4938..5762
                     /locus_tag="VE486_05970"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZA38668.1"
                     /translation="MIKGSQLVGRAVIDMEAAERLGRIKEIIVQRDGERVAGFVVVHG
                     ETIVGTGGTRQTIPASALHSIGPDAITVRGSAMKDLSPDLDSLPRMSDVIGHKMVTRS
                     GRLLGLIDDLLINGVDGTIIGFAVGEGIRSKLENIFNPQRSRIHGYVRADADLQVGNE
                     LIVVPDDALIEGEPSVQEGDHKQTTQKAGEAESRGWGARASSESTRSSIWTRRTDAAT
                     RRPSQTPAEARPESETAQPVENKRAAAPAEEATPEASPTARGDEAVTAAPQDQPPK"
     gene            complement(5843..6703)
                     /locus_tag="VE486_05975"
     CDS             complement(5843..6703)
                     /locus_tag="VE486_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZA38669.1"
                     /translation="MTRRFVILGLAVGLAVVAVVSLYRSYAALQDNDEIIPRSGESPR
                     PEAVSAGWADGNVREVQTKLREGGFYSGEIDGAYGSELALALTRYQIRNGLPITGQLD
                     VDTSKALGAKPAVATTATDRARSSETWRRLRMADQETVNNARVTPSPGASGSPLTESS
                     AANISKERLRDYVGAFVLAGLDPNVGSEADFFADRVQYYDQGVMDREKIRKDLQRYAA
                     RWPERRFWLAGDIAVEPGNEKGVRVTFPLRYDLRNGAKHSSGKINKTLVLAPAGDNLQ
                     IVAVNERKAK"
     gene            6807..>7025
                     /locus_tag="VE486_05980"
     CDS             6807..>7025
                     /locus_tag="VE486_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38670.1"
                     /translation="MPIILFALLLFVLLALGGVVLLSLALRYRAGTARRQGRRWVASL
                     NVWMTSFSAVLFLSIAFLISFWLGAAFRF"
BASE COUNT         1502 a         2056 c         1991 g         1476 t
ORIGIN      
        1 aacgatccct ggactgagca tggcatcctg aaactggaga gcgtaaagcg ttggcaaatc
       61 tttatcgatg agagatgacc gatcactaat cactcgccat tctctctcgg tcgatcgcca
      121 ggtaatcgtc ccgcggcgga ctaaacgtat cgagcacgcg tgtcggcaat aacgtttcca
      181 caccgtgcgg aacattgctc gcgagataga aagaatctcc ggtggaaagc tcgtgcggca
      241 cgtcatcaac gatgagccgc atttgacctt cgagaatgtg aacgatctgc tcctgcgggt
      301 gcgagtgcgc aggcatgcga ctcccggccg cgagcgtggc gaccatctga tacattgtct
      361 tgccgtttgc gatcgtccgg cgtgtgatgc cgggacaaat tccaattgtc ggactttctt
      421 gtttcatttc tcgcgtttgc tggttctcaa tatcatcctt cataattcgc ccttcatcct
      481 tatttgcgaa tcttacgcgg gaggattact cgcgcgaatg tgcttttgat tggtgctgtc
      541 tcgcccgctt taagcagccg cacgtgaaaa tagccaagca cgccttcccc ccagccgctg
      601 tctcagttga actcgattag cgagatattc cccgtactcg tactcagcaa tctcccaatt
      661 ctggtaggcg acgatccgcc gtccgtccca cgaccacgat ggttgagcga atccacccgg
      721 accgctggtt aattgccgca gtcccgagcc gtccgcgttg accacaaaaa tttgaccggt
      781 attggcgtgg ccggcacggt tagaactgaa tgcgatccgt ttgccatccg gtgatcagac
      841 gggccacccg tcgaaggccg cgttgttcga cagattaact tcattcgacc catctgcgtt
      901 cgccacaaac acctctgaat tgcggccaat cgcgctgagg tcccactgaa aggccggtcc
      961 ttcgatcact ttccgataaa cgatgtgctt catatcggga gagaacgatc catatgtgca
     1021 tacagtgcgc aaacgagtgt gctgcagcac accgctgccg tccggtttca tgctgaacag
     1081 ctcatgccat tgcttcgacc aatcggccgc cggatctggt gtcatgcgtg aagaattgaa
     1141 catgatgcgc gaaccgttgg ccgaccagtg cggatgcgaa tcatcgccat tggtgcgtgt
     1201 gagttgcaca atgccgctgc cgtcggcatt cataacgaaa atctccgagt gaccggcagg
     1261 ctcgccggcg aagacaattc ggctcatgtc cggagaccag cagggcactg cggcgggaaa
     1321 atcgagatag gtaagttgac gaatctcggt tccatcggcc ttgcttacgt agatttgcgt
     1381 cttgccggaa cgattggact gaaagagaag caactttccg tccttggaaa actgtggata
     1441 agagtcgatc acgttcgtca aacgtttcat cacgaccgga gcctccgaaa tagccggcag
     1501 cgcaatcgcc agccataata ccgttgcaag agaggccaat ttcatcctgt ttacggtcac
     1561 cttacccgag aggatcagtg acgcgaatgt tcgctttctt gccccgatca taagccgctt
     1621 acgatgcatg agagcagcgt aggagtgttc aagtggtgtc gcgactacgc gtggcattcg
     1681 gtgtgccgcg gatcaccgat tctcgatctt acgctgctgg cttcgatttc gtcgcggctc
     1741 tctttcgttt acgtggccgt tgccgagact ttgaagcagc tcccttacga acagcaggga
     1801 ctttccgctc ctgttcggaa gcggacgcat cgcgctggcg acgtttctgg cgttccttac
     1861 gcagcttctt ctcgcgcaaa taagtgctaa atctcttata agcaaaaccg ccgccgaagc
     1921 cggcggcagc gattgccagt gcagctgcga tcttgactgg cccacggccc ggcgccatgt
     1981 gccatggaca tttggagagc gggtatcgat ccggaagata gaacccacat ccaggtgaag
     2041 tacacttacg cgccataatt ggttatcttt ttagccagcc gcgactgaaa acaccataaa
     2101 ttatagtctc tggacgcaaa ctttggtaag tcaaaaatca tccatcgtta gccgctcacg
     2161 atgtatgcgt tccgcgaagc ggtgtagtgg cggctgtgca tgccacgccg aagccaagcg
     2221 gagccgggtc agccgcaatc tgagtgcatg tgaatgattg ctgagcgagg atcctcctcc
     2281 agttcgcgcc gcgtctccgt gcactacttc gtcacgaagg gcgggggcgc cattcgctcg
     2341 acgcagctca catcgagatt tttcgcatcg cctttctcca gaaattcgat cgcaatccga
     2401 tcaatgcaac cagcgtcgct cagaccatca acactgtggc cagcctgggg gataatgaca
     2461 tgcctgctgt tcggcaaatg cctggcgacc tcttcaccgt accttggagg tgtgaccgga
     2521 tccatgttgc cggaaaaaat cagcaccggc gcgtttgaag aaaccgggtc gagaaaatcg
     2581 gttggaatct cgccgcgcgg ccacatcccg caggcacgcg tttgctggaa cacgcgatag
     2641 ttcccgaacg gattacccgc agtcagcttc gccgcttcct ccggattaat gaaggggacg
     2701 tcttccgcgc acgtgaccga caaatacatt ccgtccgcca cgaaatccgg gatcgacggc
     2761 ccaattgctt gctggagaaa tggcgcgaga tcgccatttg ccgcacggtg aacgatcaac
     2821 ggaatccgcg ccgctttatc gcgatcgtac atccacgtcc tgattttctc cccaaataca
     2881 ccgcgctgaa tttccactgt cgtcggcgca gcattctggc gcggggaata ttccacacgc
     2941 gccggctgtt tctccaactg cgccaacaca ttcttccagt catcgcgaat ttgcgggaaa
     3001 gcggcgtgac acctcgcgtc ctgttcgcat tcgcccaaca gcaggtccat tgcgcgcgct
     3061 ccggattcgg cgtggtgtaa cggcattttc aaatcagtcg gcgcgactcc cagcaggatc
     3121 gcgctgcgca catgctccgg gtgctgccgc atgtaaacca gcgcagcgcg tgttccatac
     3181 gacgctccga aaaggctgat tctgtcgtaa tcgagccatg atcgcacttc gtccagatca
     3241 tccatcgcga tggacgttgt gtatttcgtc aggtccgcgc gttgctcgag cgtgtgacgc
     3301 atttccttga cgtgatcgat tggaaacatc tcgctcaggt aatagctcgg tgttttctcc
     3361 cgcggaattg caaggcgatt tgatttcccg gtgccgcgct gatcgacgca caccacatcg
     3421 tgccgtcggc gataaacttt gcctggtccg gcgtagaaat ctgctcggtc agcggacgat
     3481 gcgcctgggc cgcccgccag atcgaacacc ggccccgctt tcgtcttttg atcgaaggcc
     3541 ggcaggacca cgatgttcag atcgatcgtt cggccagtac gcgtttcccg gttttcaaaa
     3601 accgtcagct tcccacaaaa caattcctcg ctgattccgg gtaagcgaca cggcttcaac
     3661 tttgacaaac cacccggcaa gcgatgtttg gcgcagccgc ccgtatccag cacgaacaag
     3721 acggcgccgg ccgtcgtcag caggatcgaa caaacgcagc ggagtccgcg cacgcgcgac
     3781 cttattgctt gtgcgcaagg tcgcgcaaat tctaatgcgc gtcgtcacgg gtggctcgaa
     3841 caaagcgcac tacccgatgc ctgatgaacg cagaggcgag agcaattgcg aatcctttgt
     3901 ggacgctgcg ctggaaatcc tcagcgctag gagcaacgag tgaatacgac ggcaatactg
     3961 cttttggtga tcatcgtggc ggttatcgcg gtgggtgcat ttcttttctg gcaatggcgg
     4021 cgttcggcgg cgttgcaaca gaggttcggg cctgaatacg agcgcgctgt cggcgaattt
     4081 ggggaccaac ggaaagcgga agcggagctg gccgcgcgtg agaagcgagt gcgcacctta
     4141 gatattcgct cgctgacgcc ggaggagcag gcccgcttta ccgaagcttg gaagaaagcg
     4201 caggcgcgct tcgtggacga gccgtcccaa gccgccgctg atgcggatat tctggtgaag
     4261 aagctgatgg agacccgcgg gtatccggtg ggggatttcg agcagcgcgc ggcagacatt
     4321 tcggtggctc accccgccgt ggtgagcaat taccgcgcgg cgcgtgagat cgcgctgcgc
     4381 aacaagaccg gcaaggcgac aacagaagat atccgacagg cgatggtcca ctatcgctcg
     4441 ctatttgaag aattattaga gacagcagaa ccagcaacac gcaaagagga agggtgaata
     4501 tggaatcaaa aactgatcag ccgacgatgg aaattgccgg cgaccaaaga cgcagcgacc
     4561 agccaagttc cacggaagaa gtggcgaggg atttggaaaa ccgtaaagga acaacaggaa
     4621 aaccggccga gatcgagaag ccgacgccgt tatttcctga gaacgaagcg acgaatttcc
     4681 gcaatcgctg gacggagatc caggcggcat tcgtggatga accgcgtcgc gcggtggagc
     4741 aggcggacgc cctcgtggcg gatgtcatta aacggctggc cagcagcttt gccgatgaac
     4801 gttcaaagct cgagggccag tggggtcgcg gcgacaacgt gtcgacggaa gatttgcggg
     4861 tcgcgctgca acgttaccgc gcgttcttcg accgcttgtt gagcgtgtaa atggaatagc
     4921 gagaacggag gctggagatg attaaaggaa gtcaattggt cggacgcgcc gtgattgaca
     4981 tggaagccgc ggagcgactc ggtagaatca aggaaatcat cgtgcaacgt gacggcgagc
     5041 gcgtggctgg ttttgttgtc gtgcacggcg aaacgattgt cggaactggc ggtacgcggc
     5101 agaccattcc cgcttccgct ctgcattcga tcgggcctga cgcaatcact gtgcgcggat
     5161 ctgcaatgaa agacctatcg cctgatctcg atagcctccc gcgcatgtcc gatgtaatcg
     5221 gtcacaaaat ggtgacgcgc agcggccgac tgctcgggtt gatcgacgac ctgctcatca
     5281 acggcgtgga tggcacaatt atcgggttcg ccgtcggcga gggcatccgc agcaagctgg
     5341 agaacatttt caatccgcag cgctcccgaa ttcacggcta tgtccgcgcc gatgccgatc
     5401 tgcaggtagg caacgagcta atcgtcgttc cagacgacgc gctgatcgaa ggcgagccga
     5461 gcgtgcagga aggggatcac aaacagacga cgcagaaggc aggtgaagcc gagtctcgcg
     5521 gctggggagc gcgcgcgtca tcggaatcta cgcgatccag tatttggacg cggcgaacag
     5581 acgcggcaac gcgccgtcca tcgcaaacac ccgcagaggc tcgtccagaa tctgaaactg
     5641 ctcagccagt tgagaacaaa cgggccgctg cacccgccga agaagcaacg ccggaggctt
     5701 caccgaccgc tcgcggagac gaagcagtca cggccgctcc gcaagatcag cctccaaaat
     5761 aacgacgggc ccacaaagtg tctggtcgct gtgacggcag cgtccgatcg ccatccggaa
     5821 cacttggtaa ttcacccgcc gatcatttcg ctttgcgttc gttgaccgcg acgatctgga
     5881 gattgtcgcc ggccggtgcc agaacaagcg ttttgttaat cttaccggac gaatgtttgg
     5941 ctccgttccg caagtcgtat cgcagcggaa acgtcacccg cacgcccttc tcgttccccg
     6001 gctcaaccgc gatgtcgccg gcaagccaga aacgccgttc tggccagcgc gcggcgtatc
     6061 gctgtaaatc cttgcggatt ttttcgcgat ccattacgcc ctggtcgtaa tactggacgc
     6121 ggtcggcgaa aaagtccgcc tccgagccga cgttcggatc cagaccggcg agcacaaacg
     6181 caccgacgta atcgcgcagc cgttccttgc tgatattcgc agccgaactt tccgttaatg
     6241 gtgaaccact ggcgccaggc gacggcgtga cccgcgcatt gttgacggtt tcttggtcgg
     6301 ccatgcgcaa ccgccgccac gtctctgaac tgcgcgcgcg atcggttgcc gttgtcgcca
     6361 ctgccggctt cgcacccaac gctttgctcg tgtcgacgtc gagctgcccg gtgatcggca
     6421 gcccattacg tatctggtaa cgcgtcagcg ccaaagccag ttcgctaccg tatgcaccgt
     6481 cgatctcgcc cgagtagaaa ccgccctcgc gcaatttcgt ctgcacctcg cgcacgttgc
     6541 cgtcggccca gccggccgac accgcttccg ggcgcggtga ctcacctgag cgcggaatga
     6601 tctcatcatt gtcctgaagt gccgcgtagg aacggtacag cgaaactact gcgacaacag
     6661 cgagcccaac cgcgagaccc aaaataacaa aacgcctggt cacataattg tatcgcaggc
     6721 caaacgtcgc ctgctcttca tgaaccgacg caggtttaca agcaacgggg tcgaagcatc
     6781 tcaagcggct cgtgctacca tcagcggtgc cgatcatcct ttttgccctg ctgctttttg
     6841 ttctcctcgc cttgggcggg gtggttttgc tttcccttgc tttgcgctat cgcgccggca
     6901 ctgctcgacg gcaaggccgg cgctgggtcg ccagcctgaa tgtgtggatg acgagcttct
     6961 cggctgttct ctttctctcc atcgccttcc tgatctcctt ttggctcggg gcagcctttc
     7021 gattt
//
LOCUS       DASQUQ010000211         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1214985, whole genome shotgun sequence.
ACCESSION   DASQUQ010000211 DASQUQ010000000
VERSION     DASQUQ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214985"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1095
                     /locus_tag="VE486_05985"
     CDS             124..1095
                     /locus_tag="VE486_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38671.1"
                     /translation="MNGTDPKIEIFKPFGEAFELMKRILFQPFDLTKWLVIGFAAFLA
                     SLSTGFHSSFNPFSGWSGREDRKAMAESFRDFGSIDQMDWWVIALIVTGGLIILALVL
                     VLMWLGARGRFIFTDCIVHNHGAIVAPWKEFRVQGNSFFLFSLLVMLCIFVVVALVAL
                     ALFLPFILRGSGAQVGVGFWIALGLFLFFLLCLTFGWALVSQLMIPIMYRQRCLARQA
                     FSQSVGLVSNYPGPMLLYFLFLLVILFAASLFTCFAVCLTCCIAAIPYIGTVLLLPIP
                     VTLHGFSLLFLRQFGPDYDVWANFMPPEFSPILLSAPAPPVPLTLPT"
     gene            1215..1718
                     /locus_tag="VE486_05990"
     CDS             1215..1718
                     /locus_tag="VE486_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38672.1"
                     /translation="MNWDAISAISESVGSFAVVLSVLYLAVQVHQSTRVARLATQDAA
                     TTALRDVTKPFMENAELERIWRVGLEEIGTLSVEERARFFHAAYQFLKAFETIHFHYA
                     YGLMDRQLWDGWQGLLRHYVAAPGIAHYWKLRPEVFSERFRKFVDALEPPAEQRTVGT
                     LVEQEQK"
     gene            complement(1821..2270)
                     /locus_tag="VE486_05995"
     CDS             complement(1821..2270)
                     /locus_tag="VE486_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38673.1"
                     /translation="MKIHILLCALVITVSLSLRAGAPAQEKAFLDKYKTAFEAKDTAT
                     LESFLYAQGADPAILGFYKMMQSAEAGEKISNLELVDLTPEDAKQAATPMDSPTGGKV
                     CLTLKPSKKLVIKIERKDASGSSTNTTKNFIAEKDGKFVIPVPGPCK"
     gene            2424..3620
                     /gene="sucC"
                     /locus_tag="VE486_06000"
     CDS             2424..3620
                     /gene="sucC"
                     /locus_tag="VE486_06000"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZA38674.1"
                     /translation="MNIHEYQARELLQRFDVATTRGKVASSLDEAEQIARELGDVDVV
                     VKAQIHAGGRGKGTFKNGFKGGVHVRKTPAEVRELADKMLGQTLVTHQTGPAGRLVNK
                     VVVAESAEIAREIYFAILLDRASAAPLVVASTEGGVEIEAVAAKSPEKIIREAIDPLA
                     GLQPFQTRKLAKQLGFESSQLKSASKLFEGLYRTFIAFDCSMVEVNPLVLTNNGEVLA
                     VDAKFNFDDNALYRHPEIAAMRDIAEEDPREVEASRHGLNYIGLDGNIACLVNGAGLA
                     MATMDIIKFYGGEPANFLDVGGGATEEQVTEAFKILISDKNVKAILVNIFGGIMKCDI
                     IAQGIINAAKALKLTVPLVVRLEGTNVEAGRKLIAESKLAVIAAKDLADAAQKAVAAA
                     NPKSQE"
     gene            3622..4005
                     /locus_tag="VE486_06005"
     CDS             3622..4005
                     /locus_tag="VE486_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZA38675.1"
                     /translation="MKIKEIGFVATPVTDIKRARQFYEEVLGLKVAEQMMGGQWIEYA
                     VGGNTLAIANVSDTWTPSDQGTGAAFEVEDFDDAIKRLKDRGVPFAAEPFETPCCHMA
                     VVQDPDGNKLIIHKLKPENEKGICS"
     gene            4002..4361
                     /locus_tag="VE486_06010"
     CDS             4002..4361
                     /locus_tag="VE486_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZA38676.1"
                     /translation="MITNEVAFVAIAVSDAERARKFYQETLELTPSTIAMGGAWVEYD
                     LGPITIGVGCHPAWKPSRDGTTVAFEVADMDAAIAKLKERGVTFDIEKTETPVCWMAQ
                     FRDPDGNKLVVHKRKSK"
     gene            4438..>4974
                     /locus_tag="VE486_06015"
     CDS             4438..>4974
                     /locus_tag="VE486_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZA38677.1"
                     /translation="MSVLVDKSTRLVVQGITGSAGAFHTRQCMDYGTNVVAGVTPGKG
                     GQMFDEKVPVFDTVWEARQKMGCDASMVFVPAPAAADSILEAVDAGVELVVCITEGIP
                     VMDMMRVKALMRGEESRLIGPNCPGIITPGACKIGIMPGYIHKPGNIGVISRSGTLTY
                     EAVWQLTSRGYGQSTCIGI"
BASE COUNT         1115 a         1257 c         1434 g         1168 t
ORIGIN      
        1 ggtgtaagca tggcaatcga tgcgcttgcc ctataccgcg gttgacccga actccgaaag
       61 gcttcgcgag caggcgcttt tcggggttga tcgccatggg caaagcgttt cacaatgccc
      121 gccatgaatg gaacggaccc aaaaatcgaa atctttaaac cgtttggtga agcattcgag
      181 ttgatgaaaa ggattctttt tcagccgttc gatttgacca aatggctcgt cattggattc
      241 gcggcgtttc tcgcgagttt gtcgaccggg tttcattcct catttaatcc cttctcgggt
      301 tggagcgggc gcgaggatcg gaaagcgatg gctgaatcct ttcgcgactt tggttctatc
      361 gatcaaatgg actggtgggt gattgccctg attgtcaccg gaggcttgat catcctggca
      421 ctggttcttg tattgatgtg gctgggagcg cgcggtcgat ttatttttac cgactgcatt
      481 gtgcataatc acggcgcgat cgtcgcgccc tggaaagaat ttcgcgtgca gggaaacagt
      541 tttttcctgt tttcactgct ggtcatgctg tgcatttttg tcgtcgtcgc actcgtggcg
      601 ttggcgctgt ttcttccctt cattctcaga gggtccggcg cgcaggtcgg tgtcggtttc
      661 tggattgctc tcgggctctt cctttttttc cttctctgcc tcacgttcgg ctgggcgttg
      721 gtttcacagt tgatgatacc gatcatgtac cggcaacgct gcctggcgcg acaagccttc
      781 agccaaagtg tcggattagt ctcaaattac ccagggccga tgcttctcta ttttctgttt
      841 ctccttgtga ttttgttcgc tgccagcctg ttcacttgct ttgcggtgtg cctgacttgt
      901 tgcattgctg cgattccgta catcggcacg gtgcttcttt tgccaattcc ggttacgctt
      961 cacgggttct cgcttttatt tctgcgccag tttggacccg attacgacgt atgggcgaat
     1021 tttatgccgc ctgagttttc gcccatttta ctgtcagcgc ccgcgccgcc agtccctctg
     1081 acgcttccga catgatggaa ccagcgggag aagacattga gactgtatga ggccgctgca
     1141 atttctctgc gccgctttca tctgtgtatc ttcggttgta gcaatccgcg gtttccctgt
     1201 gatataacac ggaagtgaac tgggacgcga tcagcgccat ctcggaatcg gtcggctcgt
     1261 ttgccgttgt tttgtccgtg ttatacctcg ccgtgcaagt ccatcagagc acgcgtgtgg
     1321 ccaggctggc tacccaggat gcagccacga cagccctgcg cgacgtcacc aaacctttca
     1381 tggaaaacgc cgaactggaa cggatctggc gagtaggtct ggaagagatc ggcacactct
     1441 ccgtcgaaga gcgagcacgc ttttttcatg cggcgtatca attcttgaaa gcgttcgaga
     1501 ctatccattt tcattacgcc tatggactaa tggacaggca gctgtgggat ggctggcagg
     1561 gactcctccg gcattacgta gccgcgcccg ggatcgcgca ttattggaaa cttcgccctg
     1621 aagttttctc cgagcggttt cggaaattcg ttgatgcgct cgaacctccg gccgagcaac
     1681 gcacggtagg aactcttgtc gaacaggaac aaaaatgaaa ttcgggaaga atggtgggga
     1741 cgccgcagcg cgccgtccct atcaattcgc gatgatacgt cctcgcgtcg ctttagtgca
     1801 ctgcaaatct caccgggatg ttacttgcac ggccccggaa cgggaataac gaatttgccg
     1861 tctttctcgg caatgaagtt tttggttgtg ttggttgagc tgccgctcgc gtcttttctc
     1921 tcaatcttga tgacgagctt tttactcggc ttgagcgtga ggcaaacttt gccgccagtt
     1981 ggtgaatcca ttggcgttgc cgcctgtttc gcgtcctccg gggtaagatc gacgagttcg
     2041 aggttggaga ttttctcccc ggcttcagct gactgcatca ttttgtaaaa accaagaatc
     2101 gctgggtcgg cgccctgggc gtagaggaat gattccagcg tggctgtatc cttggcctcg
     2161 aatgctgttt tgtatttgtc gaggaaggct ttttcttgtg ccggggcgcc ggctcggagt
     2221 gaaagagaga ccgtgatgac aagtgcgcaa agcaagatgt ggattttcat aggatgaatg
     2281 agggcggggc atcctaggcg gcgggaactc agagcgacaa gccaatttgc aaaagaaaaa
     2341 tctttgctga cccgccttcc gcaggctatt ccactctgcc tgaaatcgat aggcggcggg
     2401 ctaaagaccg ccgctccttg cgaatgaaca ttcacgaata tcaagccagg gaattgctgc
     2461 aaagattcga tgtggcgacg acgcggggca aagtcgcttc gtcgctcgac gaagcggaac
     2521 aaatcgcgcg tgagctgggg gatgtcgatg ttgtcgttaa agcacagatc cacgctggcg
     2581 gacgagggaa aggcacgttt aagaatggat ttaaaggcgg agttcatgtt cgcaaaaccc
     2641 cggccgaagt gcgcgagctc gctgacaaga tgctcggaca aactctggtg acgcatcaga
     2701 ccggaccggc tggccgactg gtaaacaaag tagtggtggc ggaatcggcc gagatcgcac
     2761 gagaaatcta tttcgcgatc ctgctcgatc gggccagcgc cgcgccattg gttgtggcga
     2821 gcaccgaagg cggtgtcgag atcgaagcgg ttgcggcaaa gtcacccgag aaaatcattc
     2881 gcgaagcgat tgatccttta gccggtctgc aaccatttca aacacgcaaa ctagcaaagc
     2941 aactgggctt tgagtcgtcg caattgaaga gcgcatccaa attatttgaa gggctctacc
     3001 gcacctttat cgcgttcgat tgctcgatgg tcgaagtaaa cccactggtt ctcacgaaca
     3061 acggagaagt gctcgcggtc gatgcaaagt ttaacttcga cgataacgcg ctgtatcgac
     3121 atccggagat tgcggcgatg cgcgacatcg ctgaggagga cccgcgcgaa gtcgaagcgt
     3181 cgagacacgg tctcaattac atcgggcttg atggaaacat cgcctgcctc gtgaacggcg
     3241 ccggtctggc catggccacg atggacatca ttaaatttta cggcggcgaa ccggctaact
     3301 ttctggatgt cggtggcggg gcgacggaag agcaggtcac ggaagcgttc aaaattctca
     3361 tctccgataa gaacgtgaag gcgatcctgg tgaacatttt cggtggcatc atgaaatgcg
     3421 atatcatcgc gcaaggcatc atcaacgcgg cgaaagcgct aaaacttacc gtgccgctgg
     3481 tcgttcgcct cgaaggaaca aatgtcgagg ccggaaggaa attgatcgcc gaatcgaagc
     3541 tcgctgtcat cgccgcaaag gatttggccg atgctgcgca aaaggccgtc gcggcggcaa
     3601 atcccaaatc ccaagagtga catgaaaatt aaagaaatcg gattcgtagc gactcccgtg
     3661 acagacatca aacgggcacg ccaattttat gaagaggtgc ttggcttgaa agtcgcggag
     3721 caaatgatgg gcggacaatg gatcgaatac gcagtgggcg ggaacacgct ggcaatcgcc
     3781 aacgtaagcg acacatggac gccatccgac cagggaacgg gcgcggcgtt tgaagtagaa
     3841 gacttcgatg acgcgatcaa gcgtttgaaa gatcgcggcg tgccgtttgc agccgaacca
     3901 tttgaaacgc catgctgtca catggccgtt gtgcaggatc cagacggtaa caaattgatc
     3961 atccacaaat tgaaaccaga aaacgagaaa ggaatctgtt catgattacc aacgaagtag
     4021 catttgtcgc gatcgccgtt tccgacgcgg agcgcgctcg gaaattttat caggaaaccc
     4081 tggagctaac acccagcaca atcgccatgg gcggcgcatg ggtcgaatac gatctcggcc
     4141 cgataacgat tggtgttggt tgtcatccgg cttggaaacc gtcacgcgat ggaacgacgg
     4201 ttgccttcga ggtcgcggac atggatgcgg caatcgccaa actcaaagag cgcggcgtca
     4261 ccttcgatat cgaaaaaacc gagacaccag tctgctggat ggcgcaattt cgcgatcccg
     4321 atgggaacaa actggtcgtt cacaaacgaa aatcgaaatg aaccctgaat cgctaaggaa
     4381 agcacgaaag caggaactga ttcggattct tcctgcgttc ctacgttcct cagagatatg
     4441 tctgtcctag tcgataaaag cacgcgacta gtggtccagg gaattactgg gagcgcgggc
     4501 gcgtttcata cgcggcaatg catggattac ggcaccaacg tggtcgcggg cgtgacgccg
     4561 ggtaaaggcg gccaaatgtt cgatgagaaa gtgccggtat tcgacacggt ctgggaagcg
     4621 cgacaaaaga tgggatgcga tgcgtcaatg gtgttcgtcc ccgccccggc agccgcggat
     4681 tcaattcttg aagctgttga tgcgggcgtc gagctcgtgg tttgcatcac ggagggaatt
     4741 ccggtgatgg atatgatgcg ggtgaaagcg ctgatgcgcg gagaggaatc gcgactgatc
     4801 ggtccgaatt gcccgggaat cattacccca ggcgcgtgca agattgggat catgccgggc
     4861 tacatccata agcctggcaa tattggtgtg atctcgcgtt ccggaacgct cacctacgaa
     4921 gcagtgtggc aactaacgtc gcgcggttat gggcagtcga cctgtatcgg catc
//
LOCUS       DASQUQ010000212         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_326993, whole genome shotgun sequence.
ACCESSION   DASQUQ010000212 DASQUQ010000000
VERSION     DASQUQ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326993"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VE486_06020"
     CDS             <1..221
                     /locus_tag="VE486_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38678.1"
                     /translation="EANVSSIFKWFKGDFEKAGGVKSALAKYAPAPARPMLEKPGCKI
                     SYRPYNWGLNDHGPHGRNYKISIFDFIH"
     gene            complement(313..1269)
                     /gene="arsS"
                     /locus_tag="VE486_06025"
     CDS             complement(313..1269)
                     /gene="arsS"
                     /locus_tag="VE486_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HZA38679.1"
                     /translation="MNRFADRLAAEGLQLRRTRPEILQVNVGKLCNLTCTHCHVNAGP
                     KRKEIMTRQTIDRIIDWLAKAEIPIVDLTGGAPEMIPDFRYFIKRVKAFTPERHVIDR
                     CNLTILLEPGYRDLPEFLATHKVEIIASMPCYSSENVNAQRGEGVFEGSIKALQLLNS
                     VGYGKAVDLPLHLVYNPVGAFLPGPQAELEADYKRELKEHFGIVFNKLYTITNLPIAR
                     FASYLRHNNQLEEYMQLLIDNFNPATINGLMCRNTISVGWRGEVYDCDFNQQLDMQWE
                     NGKRLFLWDVNADKIEDREIMTGDHCFGCTAGAGSSCSGALV"
     gene            complement(1274..2425)
                     /locus_tag="VE486_06030"
     CDS             complement(1274..2425)
                     /locus_tag="VE486_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HZA38680.1"
                     /translation="MLRWRELLLMCLALLFLATCFLAYSNGANDNFKGVASLFGSRTC
                     GYRTAISWATITTFAGSIMSIFLAQTLLKKFSGKGIVPDHFTGSEYFLLAVAIGAGLT
                     VILATLTGFPISTTHALTGAIIGCGLVAVGSQVNFGALGKGFVLPLLLSPILAMAVAG
                     SLYAIFHGTRIALGLKKKWCVCIDEDERVIAMPQPTSLLALRAISLPSHLTVDEVENC
                     RERYGGNFLGFTSQQVVDAAHFLSAGTVSFARGLNDTPKIVAILLLWKALDIRWGFAA
                     VAVTMAIGGLLNARKVAETMSKKITVMNHGQGFTANLTTAILVVLASLFGLPVSTTHV
                     SVGSLFGIGLTTDKANYPMISAIVLSWLVTLPCAAILAGTTYWLAVRLV"
     gene            complement(2398..>3304)
                     /locus_tag="VE486_06035"
     CDS             complement(2398..>3304)
                     /locus_tag="VE486_06035"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZA38681.1"
                     /translation="GKVIGVDMTDEMLEVARRNAPVVAERIGYSNVEFRKGRIQDLAL
                     DLERLDRELKQNPITDATSFLAADELAEELSVKYPLIAGDSVDVVVSNCVLNLVEPKS
                     KRQLFDEIFRVLRKGGRAVICDIVSDEEVPEEMQNDPELWTGCISGTLTEEKFIGAFE
                     SAGFYGIQILNRDARPWRTVQGIEFRPVTIEAFKGKQGECFERNQAVIYRGPFKEVLD
                     DDNHRMERGRRYAVCDKTYNLYKKAPYREFFEFVDPLVDVPLAEAKPFDCSRTVLRHP
                     KETKGQDYDVTTEANNKCCDGGSCC"
BASE COUNT          780 a          912 c          868 g          744 t
ORIGIN      
        1 acgaagccaa cgtctcaagc atctttaagt ggttcaaagg cgatttcgag aaagccggcg
       61 gcgtgaaatc ggcgctggcg aagtatgcac ccgctccggc gcgcccaatg ctggagaagc
      121 cgggttgcaa gatcagctac agaccctaca actggggctt gaacgatcac ggcccgcacg
      181 ggcgcaatta caagatcagc atcttcgatt ttatccactg attcctcctg cctgcgatga
      241 ccgcttccgc ttactcaacg ttcgcggcac tcgcgcgctc ttgatttttc cagcaaggaa
      301 taatcaagcc gatcacacca aagcgccgct gcagcttgag ccggcgccgg cagtgcaacc
      361 aaaacaatga tcgccggtca tgatttcgcg atcttcgatc ttgtcggcat tgacatccca
      421 gagaaagagc cgtttgccgt tttcccattg catgtcgagc tgctgattga aatcgcaatc
      481 gtaaacttcg ccgcgccatc cgacgctgat tgtgttgcgg cacatgagcc catttatcgt
      541 agccggatta aaattgtcga tcaacagctg catgtattct tcaagctgat tgttgtggcg
      601 cagatatgag gcaaagcgcg cgatggggag attcgtaatc gtgtagagct tgttaaagac
      661 gatgccgaaa tgttccttta gctcgcgttt gtaatcggct tccagttcgg cttgcggtcc
      721 gggcaggaac gccccgacag ggttgtaaac cagatggagc ggcagatcga cagccttgcc
      781 atacccgacc gaattcaaaa gttgcagcgc cttgatgctg ccttcgaaca cgccttcgcc
      841 gcgttgcgcg ttcacgtttt cagacgagta gcacggcatc gaggcgatga tctcgacttt
      901 gtgcgtggcg agaaattccg gcagatctct atagccgggt tccagcaaaa tcgtcagatt
      961 gcagcggtcg atcacatgcc gttctggcgt gaacgctttc acgcgtttga tgaaatagcg
     1021 aaaatccgga atcatttccg gcgcgccacc ggtaagatcg acaatcggta tttcggcttt
     1081 tgccagccaa tcgataatcc gatcgatggt ctggcgcgtc atgatttctt tgcgctttgg
     1141 tccagcgttc acatggcagt gtgtgcaagt gagattgcag agcttgccca cattcacctg
     1201 caaaatctcc gggcgcgttc ggcgcagctg caaaccttcg gcagcaagtc gatcagcaaa
     1261 acggttcatg aagttaaacg aggcgcacgg cgagccaata ggttgtgccg gcgaggattg
     1321 cggcgcatgg cagcgtaaca agccaggaaa gaacaatggc acttatcatt ggataatttg
     1381 ctttgtccgt ggttaggcca attccgaaaa gtgaaccgac cgaaacgtgc gtcgtggaaa
     1441 ccggaagtcc aaatagacta gccaacacaa ccaggattgc cgtcgtcaga ttcgcagtga
     1501 aaccctggcc atgattcatg acggtgattt ttttactcat cgtctcggcc acttttctcg
     1561 cgttgagcaa gccgccaatt gccatggtga cggcgaccgc ggcaaagccc caacgaatat
     1621 cgagcgcttt ccacaaaagc agtatagcga caattttggg cgtgtcgttc aaaccgcggg
     1681 caaaactcac cgtcccggca ctgagaaaat gcgcagcatc gacaacctgc tgcgaagtaa
     1741 agcccaggaa attgccgccg tagcgttcgc ggcagttctc cacctcatcg accgtcaggt
     1801 gtgatggaag agaaatggcg cggagcgcga gcaacgaggt cggctgtggc atcgcgataa
     1861 cgcgctcgtc ttcgtcgatg caaacgcacc atttcttttt caaaccgagc gcgatccgcg
     1921 tcccgtgaaa aatcgcatac agcgagcctg cgacagccat ggcgagaatc ggactaagca
     1981 gcaatggaag aacaaaacct ttgccgagcg cgccaaaatt cacctgtgag ccaaccgcga
     2041 caagaccaca accgatgatc gcgccagtca gcgcgtgtgt cgtcgagatt ggaaatccgg
     2101 tgagcgtggc gaggatcaca gtgaggccgg cgccaattgc gacggcgagg agaaaatatt
     2161 cggagccggt gaaatggtcg ggcacgatcc ctttgccgct gaatttcttc agcaacgttt
     2221 gcgcgagaaa gatcgacatg atcgatccgg caaaggtagt gattgtcgcc caactgattg
     2281 cggtccgata accgcaggtg cgactaccga acaatgacgc gacgcctttg aagttatcgt
     2341 ttgcgccgtt ggaataggct agaaagcagg tcgcgagaaa aagaagggcc aaacacatca
     2401 gcagcagctc ccgccatcgc agcatttgtt gttggcttcg gtcgtcacgt cgtagtcctg
     2461 gcctttcgtt tctttcggat gtcgcagcac cgtgcgcgaa cagtcgaacg gctttgcctc
     2521 ggccagcggc acgtcaacca gcggatcgac aaattcgaaa aactcgcgat aaggcgcttt
     2581 cttgtaaaga ttgtaggtct tgtcgcaaac cgcgtaacgc ctcccgcgct ccatccggtg
     2641 attgtcgtca tcgagcactt ccttaaacgg cccgcgataa atcaccgcct gattgcgctc
     2701 gaaacattcg ccttgttttc ccttaaacgc ctcaatcgtg acagggcgaa attcgattcc
     2761 ttgcactgtg cgccatggtc gcgcgtcccg attcaagatt tgaattccgt aaaatcccgc
     2821 gctttcaaac gcgccgatga atttttcctc ggtgagcgtt ccggaaatgc agccggtcca
     2881 aagttctgga tcattttgca tttcttccgg aacttcttcg tcgcttacga tgtcgcaaat
     2941 taccgcgcgt ccaccttttc tcaggacacg gaaaatctca tcgaagagct gacgcttcga
     3001 tttcggctcc accagattga gcacacaatt ggaaacgact acgtccacag agtcacctgc
     3061 aatgagtgga tatttgacac tcaattcttc ggccagctca tcagccgcaa gaaatgatgt
     3121 cgcatccgtg atcgggtttt gtttcagttc tcgatctagt ctttccaggt cgagcgccag
     3181 gtcctgaatg cggcctttac gaaattccac gttcgaataa ccgatccgct cggcaacgac
     3241 cggcgcattg cggcgcgcga cttccagcat ttcgtcggtc atatcgacgc cgatgacttt
     3301 ccct
//
LOCUS       DASQUQ010000213         3427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_49229, whole genome shotgun sequence.
ACCESSION   DASQUQ010000213 DASQUQ010000000
VERSION     DASQUQ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49229"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VE486_06040"
     CDS             <1..326
                     /locus_tag="VE486_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZA38682.1"
                     /translation="VKGKAAVKDQLDRASTSIPLNIAEGNGKFAIKDRCRYLDFARGS
                     ALECAACLDVLVAKQLSNEAVITPGKEQLLEIVSMLIGLINSLTSRIGEEQGTYLASV
                     EQEEE"
     gene            348..1736
                     /locus_tag="VE486_06045"
     CDS             348..1736
                     /locus_tag="VE486_06045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZA38683.1"
                     /translation="MKFINLTRQTEIGANSYYLELGGHRLVLDCGMHPKNTGEDALPN
                     FKVIADRHIEAILISHAHQDHIGTLPVLMRRQPSARVFMTETTAEIGSVLLHNSVNVM
                     TRQREQIGPAVAGSYPLFGHRETDRASELWQWCRLRQRISISGERAPQSRMLSGLTFE
                     FFDAGHVLGSTGILLRAEDRTVFYTGDVNFDDQTIMEAAIFPEEKIDVLIMECTRGDH
                     AKPEGWTRAGEERRLAEALGRAFERGACVLMPVFALGKTQEVVAMLYKFRRERLLPEF
                     PIYIGGLSSKMTDIYDRRAHMTRRQLPRLQLMRDAAPFILNDETVRDAPLRAGRVYAL
                     SSGMMIPKTLSNVFARRLIENPQHSIFFVGYANPESPAGRLREAGSDGEVALDPDKPA
                     QRIRCNIEQFQFSAHATRESLIDYAKKLSPRKIVLVHGDPPAVEWMRATLAAVLPGSE
                     VIVPAPGVELEL"
     gene            1827..2591
                     /gene="xth"
                     /locus_tag="VE486_06050"
     CDS             1827..2591
                     /gene="xth"
                     /locus_tag="VE486_06050"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZA38684.1"
                     /translation="MRIVSWNINSLRKRQERLFAWLETTRPDIVCLQETKCTDDQFPV
                     LALEAVGYHSAYHGEKSYNGVAILAKTELRDVRPSLCDEVVDPQARVIAATISHLRVF
                     SIYAPNGQAVGSPAYEYKLHWYQRLRDCIAQEKSANVVVCGDFNVAPEDIDVYDPDLW
                     RGAIMCSDGERAAFRQLCESGLRDTLRLHHEEGGLFSWWDYRMLSFPKNRGLRIDAIL
                     ASEALAKKCIDAEIDRDMRKGKEPSDHAPVWAEFEI"
     gene            2693..>3427
                     /locus_tag="VE486_06055"
     CDS             2693..>3427
                     /locus_tag="VE486_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HZA38685.1"
                     /translation="MANESHLRVSNPPPRPLMIWDGECHFCKRWIERWREITAGKVDY
                     VTYQEAANRFPEIPIEQFKRAVAFIEPDGNAFFAAKAVYRSLRYRSSRRWLAWSYDHV
                     PGFAAVSETAYKFVARHRGAGSAVTRLLWGKDVRPPTYFWARRWFLRALGLIYLIAFV
                     SLWVQVDGLVGTDGISPINQFLPAAHAQLGNRAYSLLPTLCWFNSSNAFLHFLCAGGI
                     ALSLLLMAGFVPVVSLITLFVFYLSLT"
BASE COUNT          742 a          919 c          964 g          802 t
ORIGIN      
        1 acgtcaaagg caaagctgct gttaaagatc aactcgaccg cgcgtcgaca agtattccac
       61 tcaatatcgc cgagggaaac ggcaagtttg cgattaagga tcgttgtcgg tatctggatt
      121 tcgcacgagg atcggcgcta gaatgcgcag cttgcctcga cgtcttggtg gccaagcagc
      181 tttccaatga ggcagtgata actcctggca aggaacaatt gcttgaaatt gtctcgatgt
      241 taatcggcct gatcaacagc ctcacttccc gaatcggcga agaacaaggc acgtatttgg
      301 cttcggtgga gcaagaggaa gagtaagagc aagaggaaga gttcttcttg aagttcatta
      361 atctcacacg ccagactgaa atcggtgcga actcgtatta tttggagctc ggcgggcacc
      421 gcctcgtgct cgattgcggg atgcatccga agaacaccgg tgaggatgcg ctgccgaatt
      481 tcaaagtcat cgctgaccgg catatcgaag cgatcctgat ctcacacgcg catcaggacc
      541 acatcgggac attaccggtg ttgatgcggc gtcaaccaag cgcgcgagtt ttcatgaccg
      601 aaacaacggc cgagatcggc agtgtgcttt tacataactc cgtcaatgtg atgacgcgac
      661 agcgcgagca gatcggtccg gcagttgccg gctcgtatcc gctgtttggt catcgcgaaa
      721 ctgatcgcgc atcggaactt tggcaatggt gtcgcctgcg gcaacgcatc tccatttctg
      781 gggagcgtgc gccacagtcc cgaatgcttt cgggcttgac gtttgagttc ttcgacgcag
      841 gacacgtgct cggctccacc ggaattctgc tgcgcgctga agaccgcacc gttttttaca
      901 caggcgacgt caattttgac gaccaaacaa tcatggaagc ggcgattttt ccggaagaaa
      961 aaatcgacgt cttgatcatg gaatgcaccc ggggtgatca cgcgaaacct gaaggctgga
     1021 cccgcgctgg tgaagagcga cgcctggcag aagcgctcgg cagggcgttt gagcgcggcg
     1081 catgcgtgct catgcccgtt ttcgcgctcg gcaagacaca ggaggtcgtg gcgatgcttt
     1141 acaaattccg ccgtgaaagg ctcctgccgg agtttccaat ttatatcggc ggtctcagct
     1201 cgaagatgac ggatatctac gatcgccgcg cgcatatgac tcgccggcaa ttgccgcgtc
     1261 tgcaattgat gcgggacgca gcgcccttca ttctaaatga cgaaacagtg cgcgatgcgc
     1321 cgctgcgggc gggacgcgtt tacgcccttt ccagcgggat gatgatacca aaaacacttt
     1381 ccaatgtttt tgcacggcga ctaatcgaaa atccacagca ctcgatcttt tttgtcggct
     1441 acgccaaccc ggaatctcct gccggccgtc tgcgcgaggc aggctcagac ggggaggtcg
     1501 ctcttgatcc cgataaacca gcccaacgca tccggtgtaa catcgaacaa tttcaattta
     1561 gcgcgcacgc gacgcgcgaa tcgctaatcg attacgccaa gaaactttcg ccccgcaaaa
     1621 ttgtgctcgt tcacggcgat ccaccggcgg tcgaatggat gagagcgact ttggcggctg
     1681 ttcttcccgg ttcggaggtc attgtgccgg cgccgggtgt ggagttagaa ttgtgatcag
     1741 tgaactgtga ttggtgatca gtataaactt tggcagcgcg tcaacgtccg atcactgatc
     1801 acggttcact gatcacgttg ctacgcatgc ggatcgtttc ctggaacatc aattcactgc
     1861 gcaaacgcca ggagcgcctg tttgcctggc tggagacgac caggccggac atcgtttgtt
     1921 tgcaggaaac aaaatgtaca gatgaccaat tcccggtgct cgccttggag gccgtcggtt
     1981 atcattcagc ttatcacgga gaaaaatcgt acaatggcgt agcgatcctc gcgaaaaccg
     2041 agctgcgcga cgtccgcccg tcgctctgcg atgaagtggt cgacccacag gcgcgggtga
     2101 tcgcagccac aattagtcac ctgcgcgtgt tttcgattta cgcacccaat ggccaggctg
     2161 ttgggtcgcc agcctacgaa tacaaattgc actggtacca acggctgcgc gattgcatcg
     2221 cgcaagaaaa atctgccaat gtggtcgtgt gcggcgattt caacgtcgcc ccggaagaca
     2281 tcgatgttta cgatccggac ctgtggcgag gagcgatcat gtgttctgat ggagagcggg
     2341 cggcgttccg tcagctatgc gagtccggtc tccgggacac gctgcggctg catcacgaag
     2401 aaggcggcct ttttagttgg tgggattacc gaatgctttc ctttccaaag aatcgcggtt
     2461 tgcgcatcga cgcaatccta gccagcgaag cgttagcaaa gaaatgtatc gatgctgaaa
     2521 tcgatcgtga tatgcgaaag ggaaaagagc cttccgatca tgcgccggtc tgggccgagt
     2581 ttgagattta gcagctggca aagtcgatcc gggattaaaa aagcgctcaa cgcccaatgg
     2641 agcgggtttc tgagttggac gttggacgtt agacgttgga cgttgggcgt tgatggctaa
     2701 cgaatcgcat ctgcgcgttt ccaatccccc gcccaggcca cttatgattt gggatggcga
     2761 atgtcatttt tgcaagcgct ggatcgagcg ctggcgcgaa atcaccgctg gcaaagttga
     2821 ttatgtaacc taccaggaag cagcgaaccg atttccggaa attcccatcg aacaattcaa
     2881 acgggcggtc gctttcatcg aaccagatgg caacgccttt tttgccgcga aagctgtcta
     2941 tcgctcgtta cgttatcgct cttccagaag atggctggcc tggagttatg atcacgttcc
     3001 cggattcgcg gcggtttccg agaccgctta caagtttgtc gctcgtcatc gcggcgccgg
     3061 ctcggcggtt acccgattgc tctggggaaa agacgtccgg ccgccgactt atttttgggc
     3121 tcggcgctgg tttcttcgcg cgctcggcct catttactta atcgcattcg tttctctttg
     3181 ggtgcaggtg gacgggcttg ttggcacaga cggaatctcg cccataaatc aatttctgcc
     3241 ggcggctcac gcacaactcg gcaatcgcgc ttactcgctg ttgccgacgc tttgctggtt
     3301 caattcgagc aacgcatttc ttcattttct ctgcgccggc ggcattgcgt tgtcgctcct
     3361 tctaatggct ggattcgtcc cggtggtttc gctcatcacg ctgtttgttt tttacctgtc
     3421 gctcacg
//
LOCUS       DASQUQ010000214         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_328449, whole genome shotgun sequence.
ACCESSION   DASQUQ010000214 DASQUQ010000000
VERSION     DASQUQ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328449"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VE486_06060"
     CDS             <1..548
                     /locus_tag="VE486_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZA38686.1"
                     /translation="TGSEFSICDVTVQTFPVPHDAVDPLGFAFYAGSSGLGFITDLGY
                     ATRMVIERLREVQTLVIETNHDEKLLQNDSHRPWPVKQRIQSRHGHLSNMAAVSVIEE
                     LLPGKIQRIVLGHLSRDCNTPELALGAVRALLAKCGRIGMEVHCASQSEISPRFRIGE
                     TKPGSFQPTFENAFFQTASAL"
     gene            611..1123
                     /locus_tag="VE486_06065"
     CDS             611..1123
                     /locus_tag="VE486_06065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZA38687.1"
                     /translation="MIIRNAVEADLPAIVDIYNATVPTRMVTAELEPTTVEARLPWFR
                     EHSPDQHPFWVMECDGGVVGWLDFKKFLPRCAYRGTAEISVYVHEKFRRRGVARGLLE
                     EAIVRASSLKISAMIGLIFAHNEPSLKLFERLDFHRWGLLPRIARLDGIERDLIIMGR
                     HIRRRGSAGR"
     gene            complement(1308..1685)
                     /locus_tag="VE486_06070"
     CDS             complement(1308..1685)
                     /locus_tag="VE486_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38688.1"
                     /translation="MVAKLDGPQFSGGANIAIKCPSHTYEQTVAFYRDTLGLPLLEEE
                     KDGCIFQFGPNRLWIDNVPNLSQPDIWLELETNDTEAAASFLKVHGVTRRDEIEQLRE
                     GFDGFFVSAPNGIVHLVIGHEEA"
     gene            complement(1884..3293)
                     /locus_tag="VE486_06075"
     CDS             complement(1884..3293)
                     /locus_tag="VE486_06075"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HZA38689.1"
                     /translation="MTDRPTRVEKDSMGEMSLPASALHGASTHRAVLNFPVSGYRFSR
                     PFIRGLGLIKWAAAQANHDLGLLDAERAALIVQASEEVVDGKLDDHFPLDIFQTGSGT
                     STNTNANEVIANRCAQLAGKPIGSRELIHPNDHVNMGQSSNDVIPSAIHISAAEQLKN
                     CLIPALEKLHGALDAKAKEFWEILKIGRTHLMDATPVRLGQEFSGYAQQVAYAKERAQ
                     KSIEVLRELALGGTAVGTGLNRHVDLPGKMLRHLEQRTGIAFYEAKNHFEAQGGKDAV
                     IEASGHLKTIAVSLFKIANDIRWLGSGPRCGIGEIQLPATQPGSSIMPGKVNPVMCES
                     MMMVCAQVIGNDTTITWAGANGNFELNVMMPVMAHNILESIRLLANAIDVFCDKCVSG
                     IVANEERCRELIELSMAMVTSLAPKIGYDRAAEIAKESAKTGRTVREIAREKKVLSET
                     ELESALDPVKMTEPGGKDE"
     gene            complement(3290..3913)
                     /locus_tag="VE486_06080"
     CDS             complement(3290..3913)
                     /locus_tag="VE486_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZA38690.1"
                     /translation="MSALFFKRFLKRPFQVASIIPSSKAMVERVASKMDFSQPRVIAE
                     YGPGEGVHSREIARRMSPDSQLLLFELDPALARDLERQFAGDARVHVIHGDAASLPFE
                     LNRRGIACCDYILSGIPFSILKIDKKRALLRKTYDALAPGGSFIIYQVTNELKQHATL
                     FEHGESEYFLQNMPPMFITVFHKVPLNDREHRTGRRRSAAVCADRSA"
BASE COUNT          855 a         1233 c         1167 g          957 t
ORIGIN      
        1 ggaccggttc ggagttttct atctgcgacg tcacggtgca gacgttcccg gtgccgcatg
       61 acgctgtcga tcccctggga tttgcctttt acgccggatc gagcggcctc ggtttcatca
      121 ccgatctggg ttacgctacg agaatggtga tcgagcgatt gcgcgaagtg cagacgctcg
      181 tgatcgaaac caatcatgac gaaaaacttt tgcagaacga ttcgcatcgg ccgtggccgg
      241 tgaaacagcg gatccaatcc cggcacggcc acttgtcgaa tatggcagca gtaagtgtga
      301 tcgaggaatt gttgccggga aaaatccagc gcatcgtgct cggccacctc agccgcgatt
      361 gcaacacgcc cgagcttgcg ttgggcgccg tgcgcgcgtt actcgcgaaa tgcggcagga
      421 tcggcatgga agtccattgc gcctctcaat cggaaatcag tccgcgcttt cgaatcggtg
      481 aaaccaaacc cggctcgttt cagccgacgt tcgagaacgc gtttttccaa accgcatccg
      541 ccctgtagtg gctgtgcatg gctgccgcca tatgggaaat gcggcggtcg cagaccgccg
      601 ccacagacaa atgatcatcc gcaacgccgt cgaagcggat ttgcctgcca tcgttgatat
      661 ttacaacgcg actgtgccaa cgcgcatggt aacggcagaa cttgagccga ccacggtcga
      721 agcgcgtttg ccgtggttcc gcgaacactc acccgatcaa catccattct gggtgatgga
      781 atgcgacggc ggcgtggttg ggtggctgga tttcaagaaa ttcctgccgc gttgcgcata
      841 tcgcggcacg gccgaaatca gcgtttacgt ccatgagaaa ttccggcgcc gtggcgtggc
      901 gcggggtttg ctggaggaag caatcgtccg tgcgtcctcg cttaaaatct ccgcgatgat
      961 cggactcatc tttgctcaca acgaaccgag cttaaagctg tttgagcggc tggacttcca
     1021 ccgctggggc ttgctgccgc ggattgcgcg gctcgacgga atcgagcgcg atctcataat
     1081 catggggcga cacatccggc gtcgcggctc tgcaggacgg taagatcgac aagtctgtgg
     1141 attggggagc gcacgccgct ggcgtgcctc gtttggctgc tggccaaacg agctttcgac
     1201 gaacaccgcg aaagcttgct tgttcgtact cgaacgagtc cacttacgat ccgagagttt
     1261 tcggccagcg gccgaaaaca gcacgcgtcc gcccgcgctg cccagagcta cgcttcttcg
     1321 tggccgatca cgagatgaac aatgccgttt ggcgcggaca cgaagaagcc gtcgaagcct
     1381 tcgcgtaatt gttcgatttc atcacgccgc gttacgccat gcacttttag aaatgacgcg
     1441 gcggcttcgg tatcgttggt ttccagttcc agccagatgt ccggttgact cagattcggc
     1501 acgttgtcga tccaaagccg gttcggaccg aattggaaaa tgcagccgtc cttttcttcc
     1561 tcaagcaaag gcaaccccag cgtatcccgg taaaatgcga ccgtctgctc atacgtgtga
     1621 ctcgggcatt tgattgcgat gttcgctcca ccggaaaatt gcggcccgtc aagtttagca
     1681 accataggtc agtcataatg acgaactccg gatgccgaaa tccgaatcac cgcgcgtgtt
     1741 gttgccgcct cgccgtgccg aggcgctcat cgcgtcatcc cgccctgcac ggcgacacag
     1801 cgccggggct acaacagcct gcttctttcg tcattcgccg cttcgtcatt ccttcgtcct
     1861 tcgtcatttg ttattcgtca ttactactcg tcttttccgc ccggctcggt cattttgacg
     1921 gggtcaagcg cgctctcgag ttctgtttcc gacaacactt ttttctcccg ggcgatctca
     1981 cgaacggtgc gcccggtttt cgcgctttcc tttgcgattt cagcggcacg atcgtaaccg
     2041 attttcggtg cgaggctcgt caccatcgcc atcgacagct cgatcaactc ccggcaacgc
     2101 tcttcgttcg ccacgatacc gctaacgcat ttgtcgcaga aaacatcgat cgcgttcgcc
     2161 agcagccgga tcgattcgag aatattatgc gccatcactg gcatcatcac gtttaattcg
     2221 aagtttccgt ttgcaccggc ccaggtaatg gtcgtgtcgt tgccgatcac ctgtgcgcac
     2281 accatcatca tcgattcgca catgaccggg ttgacctttc ccggcatgat ggaactgcct
     2341 ggttgcgttg ccggaagctg gatttcgccg atgccgcaac gcggcccgct cccaagccag
     2401 cggatgtcat tcgcgatttt gaacagactg accgcgatcg tcttgaggtg gccgcttgct
     2461 tcgataaccg cgtccttgcc cccttgcgct tcgaaatgat tcttcgcctc gtaaaaggcg
     2521 atgccggtcc gctgctccag atgccgcagc attttccccg gaagatcgac atggcgattt
     2581 aggcccgtgc cgaccgctgt tccgcccagc gccagttcgc gcaaaacttc gatcgatttt
     2641 tgggcacgtt ctttggcgta agcaacctgc tgcgcgtagc cggaaaattc ctgtcccagc
     2701 cggactggtg tcgcgtccat caaatgggtc cggccaatct taaggatttc ccaaaattcc
     2761 tttgccttcg catcgagcgc gccgtgcaat ttttccagcg ccggaatcag gcagttcttt
     2821 agctgttccg ccgcgctgat gtgaatcgcg gaaggaatca cgtcgttact tgattggccc
     2881 atgttcacgt gatcgttcgg atgaatcagt tcgcgtgacc cgatcggttt gccggcgagt
     2941 tgcgcgcaac ggttggcgat tacttcgttc gcgttggtgt tggtgctcgt accggagccg
     3001 gtctggaaaa tgtcgagcgg aaaatggtcg tcgagcttgc catcgacaac ttcttcagat
     3061 gcctgaacga tcagcgccgc gcgctccgcg tcgagcaggc ccagatcgtg attcgcttgc
     3121 gcagctgccc atttgatcaa gcctaggccg cgaataaaag gccgcgaaaa ccggtaaccg
     3181 cttaccggaa aattcaggac cgcccggtgg gtcgaggcgc catggagcgc cgaggccggg
     3241 agcgacatct cgcccatcga atccttttct acccgggtcg gccggtctgt catgccgaac
     3301 gatctgcgca aacagctgcc gaccgccgcc gcccggttcg atgctcgcga tcatttagcg
     3361 gcactttgtg aaaaacggta atgaacatcg gcggcatgtt ttgcaggaaa tattccgatt
     3421 cgccgtgctc gaaaagtgtg gcgtgctgct tcagttcatt ggtcacctgg taaatgatga
     3481 aactgccgcc cggtgcgagc gcgtcatagg tcttgcgcag gagcgcacgt ttcttgtcga
     3541 tcttaagaat gctgaatgga atgcctgaaa ggatgtaatc gcagcaagcg atcccgcgcc
     3601 ggttcaactc gaatggcaaa gaagctgcat ccccatgaat cacatgtacc cgggcatcgc
     3661 ccgcaaattg acgttcgaga tcgcgtgcga gcgcggggtc cagctcgaac aggagtaatt
     3721 gcgaatccgg actcatgcgc cgggcgatct cgcgcgaatg caccccttcc cccgggccgt
     3781 actcggcgat cactcgcggc tgactgaaat ccatcttact ggccacccgt tccaccatcg
     3841 ctttcgagct ggggatgatc gatgcgactt gaaacggtcg ctttaggaaa cgtttgaaaa
     3901 agagcgcact cacgggaatc gaacagtcgt cccgtgcgtc cgagttgtcc agtggcgaac
     3961 tgcttggcgg aacaccgcga accagacccg attttggggc tgacaaggga acccataaga
     4021 atggattagc gcttcgacaa atgacgaatg tcgaatgacg aaggacaaag gaatgacgaa
     4081 atccgaatga cgagaaaaac actcgccttg ttcattcgtc atttggtcat tcgggcttcg
     4141 tcatttcggc ctcttgactg atcaccgatc accgatcact tatcacttct gatttaagcg
     4201 cgcggttagc tc
//
LOCUS       DASQUQ010000215        11782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_867720, whole genome shotgun sequence.
ACCESSION   DASQUQ010000215 DASQUQ010000000
VERSION     DASQUQ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867720"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            731..1591
                     /locus_tag="VE486_06085"
     CDS             731..1591
                     /locus_tag="VE486_06085"
                     /inference="COORDINATES: protein motif:HMM:NF005214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZA38691.1"
                     /translation="MPKEKKLQPPQHQKRQPGREYKMKPRPKAVDENQRGSGKLRDKV
                     AIITGGDSGIGRAVAIAFAKEGADVAVVYLEEHKDAKETRRLVEKEGGKCLLIDGDVG
                     DEEFCRKAVAQTEQRFGGIDILVNNAAEQHLQESIDKITARQLERTFRTNIFSFFFMT
                     KAAVKHLKKGATIINTTSVTAYKGSPHLLDYSSTKGAITSFTRSLSQALAKKGIRVNG
                     VAPGPIWTPLIPSSFPEKDVATFGSDVPLGRPGQPEEIAPSYVFLASEDSSYMTGQIL
                     HPNGGTIVNG"
     gene            2025..3074
                     /locus_tag="VE486_06090"
     CDS             2025..3074
                     /locus_tag="VE486_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZA38692.1"
                     /translation="MPLTNAKLAELLARQSETESGILVRAFRRAARNALFWPESATTL
                     VAQNRPLVELSAIGPFIGKRVRHWIENPETVLGTTPPIRRDFFALADAKSFLKKKPEW
                     EKSLRGDLQMHTCWSDGSGTVAAMAAAARERGYEYIAITDHSKGLKIAGGIDEPALRK
                     QASEIAETNRAILRSGGKLTVLRSIEMNLNPRGEGDMDAKSLSGLDLVLGSFHSSLRR
                     TDDQTERYLAALRNPHIQILAHPRGRIYNFRLGLKADWPRVFAEAVRLDKALEIDCYP
                     DRQDLNLTLLKLARSAGTRVSLGTDAHHPWQLEFIQLGLAAALRARIPAERIVNFMSI
                     QQIKGWIARLRERSR"
     gene            3098..3619
                     /locus_tag="VE486_06095"
     CDS             3098..3619
                     /locus_tag="VE486_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HZA38693.1"
                     /translation="MGFGKSFALQPRPEGAAILYEKMNEKAANRADQTPRSFRCKVND
                     LFRAFSRNIAAAVGSAWAFIVAITVVIVWAATGRIFHFSDTWQLIINTGTTIVTFLMV
                     FLIQNTQNRDGKAIQLKLDELIRALRGARNKLVDLEDLSDEELKALQKEFQRLQKQSR
                     EADTDVAKTESRR"
     gene            complement(3780..4304)
                     /locus_tag="VE486_06100"
     CDS             complement(3780..4304)
                     /locus_tag="VE486_06100"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZA38694.1"
                     /translation="MKLNTLKTLYINELRDLYNAENQLLKALPKMAKAASSEELKDAF
                     QKHLEQTKSHVERLEEVFGEIGEKPKGKTCRAMKGLIEEGSEILHEDGEDSVIDAGII
                     VAAQKVEHYEIAGYGSVRTFAQLLGKDRSAELLQTTLDEESETNEALNQLAEGIVNPE
                     AIMETELTGAGSNR"
     gene            4586..5605
                     /locus_tag="VE486_06105"
     CDS             4586..5605
                     /locus_tag="VE486_06105"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZA38695.1"
                     /translation="MLLKPPLPPMEARSVEEIPAGNSWQYEPKWDGFRCLAFRDDQKI
                     HLQSKAGQPLGRYFPDVADALARLPEQQFVLDGELVVPVSGVLSFDELQLRLHPATSR
                     VQKLAKAHPAMYIVFDLLAEKGQVYLQRILRERRRLLKRFARLNFRSAKHLRLSPATT
                     NHRTASKWFKKAGGDLDGIIAKRLDAAYASGERTAVVKVKQIRTVDCVVGGFRYARGT
                     RVVGSLLLGLYGEDGLLHHVGFTSAFKASERRALTRKFEALKKPPGFTGSAPGGPSRW
                     STDRTSEWEPVIPRVVVEVAYDHFTGGRFRHGTKFLRWRLDKKPRQCTMDQVEHREGK
                     SLALL"
     gene            5825..6010
                     /locus_tag="VE486_06110"
     CDS             5825..6010
                     /locus_tag="VE486_06110"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZA38696.1"
                     /translation="MTNLQFKGSWNEAKGKLKQKYGQLTDDDLAFAEGKDDELLGRLQ
                     KKLGKTKEELRKEIESL"
     gene            6180..6326
                     /locus_tag="VE486_06115"
     CDS             6180..6326
                     /locus_tag="VE486_06115"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZA38697.1"
                     /translation="MLWTIFVILLVLWLLGFLGHVGGGLIHLLLVIAVVVLIINLIQG
                     RRSL"
     gene            6573..6872
                     /locus_tag="VE486_06120"
     CDS             6573..6872
                     /locus_tag="VE486_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38698.1"
                     /translation="MLTATFLAAAALLATASLFLAIAIALLAAALLAAALLAAALLTA
                     TALFVTITIVLLAATLLFGACRSDGFIRIALCFHCYLSLLMIGLSHFATRPLLFL"
     gene            complement(6955..7482)
                     /locus_tag="VE486_06125"
     CDS             complement(6955..7482)
                     /locus_tag="VE486_06125"
                     /inference="COORDINATES: protein motif:HMM:NF010248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HZA38699.1"
                     /translation="MTKKVLLTGRPGCGKTTLIKRVVNNLPRRAGGFYTEEIREAGAR
                     VGFKVVTLGGKEAVFAHVDFKTPDRLGKYGLDLSALERIGVGAVRRAVRTRRLVVIDE
                     IGPMEIRSAIFRDSVTEVLDSEVPVIATIYARSLPFTDAIKSRPDVTLIEVRLGNRER
                     LVSELSDRIQELNPC"
     gene            7630..8115
                     /locus_tag="VE486_06130"
     CDS             7630..8115
                     /locus_tag="VE486_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZA38700.1"
                     /translation="MATNHDIINELRKSYAMELETVENYLANAIDLDGVRAEEIKKAL
                     LRDIEEELGHARKLGNRIKVLEGRVPGSLDLGRGQRYLQPPEDSTDLIAVIRGVIRAE
                     EEAIDEYKKIIKMCDPIDLVTQDLILDITAQEQAHRRQFIGYLYEYERGEAKRLTAAA
                     A"
     gene            complement(8222..9022)
                     /locus_tag="VE486_06135"
     CDS             complement(8222..9022)
                     /locus_tag="VE486_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZA38701.1"
                     /translation="MPELPDVETFKRYLDATSLHQRIADVDVRSAYVLKGISARELAR
                     RLKGRGFESSRRYGKHLFVRMDSDVWLRLHFGMTGSLRYFKGEEKAPPHTRVLFAFEK
                     DYSLAFDDQRKFGEVGLIEDVDAFLKKRALGPDALDVELKEFQGLFAKHRGAVKSILL
                     NQRLIAGIGNIYADEILFRAHMHPATEISALNDKAIAKLFRAMRYILENAIESKADVD
                     RTPKSWLLPHRGNGGRCPRCGRNLRSAQIGGRTAWFCARCQKKRAFGT"
     gene            9124..11517
                     /gene="ppsA"
                     /locus_tag="VE486_06140"
     CDS             9124..11517
                     /gene="ppsA"
                     /locus_tag="VE486_06140"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HZA38702.1"
                     /translation="MTAKKFVLGFDKVSSKDTNRVGGKNAALGEMIRTLKRKGVHVPN
                     GFATTADAFRLFVRENKIDKKIRAEIQRLKKGSKSLATAGKALRSLFLKGKFPEHVEE
                     QIREAYRELCERYKKRALDVAVRSSATAEDLPEASFAGQQETFLNISGEDGVVDACRK
                     CYASLFTDRAISYREEKGFDHLEIALSAGVQKMVRADKAGAGVMFSIDTESGFPNVVR
                     ISAAWGLGEVVVKGAANPDEYMIFKPLLGKRNTQPILEKLLGDKKRKVVYASGSRRSG
                     HMTRTVNTTKKERESFVLDDGEILRLAKWACAIEKHYRKPMDIEWAQEGDTGEIFILQ
                     ARPETVQALKEAGSLTTYRLKGKGKRLFSGLSIGEAIAAAKVCRIQSARDLGKFKEGS
                     ILVTEMTDPDWGPIFKKTKGIITDLGGRTCHAAIVSRELGIPAIVGTGEATKTLRDGQ
                     EITLSCAEGDEGFVYEGLLDFAQEKVSLKNLPETRTPIMLNIASPAGAFRWWKLPCDG
                     IGLARIEFIIANRIQIHPMALAHFDELKDRAARKRIQILTHGYKNKGDYFIDKLAQGI
                     ARIAAVRHPHPVIVRLSDFKTNEYAALIGGEQFEPPEQNPMLGLRGASRYYHERYRDA
                     FRLECEAIKRVRGDIGLKNVIVMVPFCRTLGEADKVLHALAENGLVRGRGGLEIYMMC
                     EIPSNAVLAEKFAERFDGFSIGSNDLTQFVLGVDRDSAALAELFDERNEAVKNVVHDV
                     IRRAHKTDCKVGICGEAPSNYPEFAAFLVECGIDSISINPDRFVETKRIVARAEQNR"
     gene            11517..>11782
                     /locus_tag="VE486_06145"
     CDS             11517..>11782
                     /locus_tag="VE486_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38703.1"
                     /translation="MSDQVEKFIRLLKESPAGAVFNPWWQVDEENDAGRNAPAIRRKQ
                     LSAYLSKRLKKARLAVIGEALGYRGGHFSGIPMTSERILLGKKK"
BASE COUNT         2782 a         3151 c         3320 g         2529 t
ORIGIN      
        1 tggacggcga aataattccc gaaaggagat gccatgggca caattctgtt aattatccta
       61 atcctcctat tgattggggc gctaccagcc tggccctaca gttcaggctg gggatattgg
      121 ccaagtggag gactaggttt aattcttttg attttaatca tcctcgcctt gacgggacat
      181 atctgagcga aagttcggac aggatattga tgcccggaaa gcgctctgaa tttcgccaca
      241 tccgatagag cggtcggcgg cgaaactatt cgaggtaatc tactatgcca gaagaagaac
      301 caatcgaacg ggcgcgcgaa gatgaacgcc agggcctgtc gccatccact caggcgggag
      361 aattcgtgcg cgaggaaata gagcacatac gggaaggtga acacggcgca cgctcgccgg
      421 agcaggccat agccattggt ctctcgaaag cacgaagggc gggtgtaaaa ttgccgccac
      481 cgaagcgtgg caaggcgcga acgaagcgcc aagccaagcg cgatctcgcg aaaggccgcc
      541 ggcccaaaaa accctctcga agacgttcac gtgcagtacg tggcgcactc aagggcgaga
      601 gccggcgatc tgcatcgcgg cgagcactat cgcaacaggc caaacgcgct gcacgccggc
      661 gttcggtggc ttcacgctcc gctgcggcaa agaaggccgc gcgaacgcga aaacgtcgtc
      721 ggcgctaaga atgcccaaag aaaagaagct tcagccgccg cagcatcaaa agcggcagcc
      781 cggccgcgaa tacaagatga aaccgcggcc gaaggcggtc gacgaaaatc agcgcggcag
      841 cgggaaactg cgagataaag tcgccatcat cactggcgga gacagcggaa tcggccgcgc
      901 ggtcgcgatc gcatttgcca aagaaggcgc agatgtggcc gtcgtttatt tggaggagca
      961 caaagacgcg aaggaaacgc ggcggcttgt agaaaaagag ggaggcaagt gcctgctcat
     1021 cgatggcgat gtcggcgacg aggaattttg caggaaggca gtcgcacaga ctgagcagcg
     1081 gttcggtggg atcgacattt tggtcaataa tgcagcggag caacatctgc aggagtcaat
     1141 cgataaaatt actgcgcgcc agctcgagcg aacgtttcgg acgaacattt tttctttctt
     1201 cttcatgacg aaggccgccg tgaagcattt gaagaaaggc gctacgatta ttaacaccac
     1261 ttcggtgaca gcctacaaag gcagcccgca cttgcttgat tactcgtcca cgaaaggagc
     1321 aatcacttcc tttactcgat ctctgtccca agccctggct aagaaaggca ttcgggtcaa
     1381 cggtgtcgcg ccgggtccaa tttggacccc gcttatcccg tcgtcgtttc cggaaaaaga
     1441 cgtggcgaca ttcgggtcag atgttccgct tggacgcccg ggccagccgg aggagatcgc
     1501 tccgagttac gtctttctgg cgtccgagga ctcgtcatac atgactgggc agattttgca
     1561 tccgaatggc gggacgatcg taaacggata gcaccaagcg gagcgctcgg ttgatcctgc
     1621 gaaccgtcct gcgttgatag gatgggataa gtctagatgt gggcgcgcga aagtgaccgc
     1681 atcgcccgtt cgagctgaca aaataaatcc gcgagcggta tcgaatgcga tttttttaac
     1741 agaaaaaagg gaggtattct atggcgcgag gaagcaagaa aaaatataca tcgaaacaaa
     1801 aacgcagggc atcgaagatt gaacgcggtt acaaaaagcg cggtgtgagc agcaaggaag
     1861 cggagcggcg cgcgtgggcg acggtaaaca aggagagccg aggcgggaag aaaagcggta
     1921 gcgggcgagg gaaaaaagaa aggagaactg cgtcgcgcaa aggtgggcgt aaaggcggtc
     1981 gcaaataagg attgctgttt tgcagcgcgg ttcaaaagca tgccgtgccg ttaacgaacg
     2041 cgaaactggc cgaactactc gcgcggcaat ctgagactga atccggcatt ctcgtccgcg
     2101 cattccgacg cgctgcgcgc aacgcgctgt tttggcccga gagcgcgact actctcgtcg
     2161 cgcaaaaccg gcccctcgtt gaactgagcg caattggtcc gtttatcgga aaacgcgtcc
     2221 ggcactggat tgaaaatccg gagactgtgc tcggaacgac tccgccgata aggcgcgatt
     2281 tttttgccct ggcggacgcg aaatcttttc tgaaaaagaa accggaatgg gaaaagtcgt
     2341 tgcgaggtga tctgcaaatg cacacgtgct ggagcgatgg gtcaggcacg gtcgctgcaa
     2401 tggcggctgc cgctcgcgaa cgcggctacg aatatattgc gatcactgat cactccaaag
     2461 gattgaaaat tgcaggtggg atcgacgaac cagccctgcg caagcaagca tccgagatcg
     2521 ctgagaccaa tcgagcaatt ttgcgcagcg gcggaaaact aacggtgctt cgttccatcg
     2581 aaatgaattt gaatcctcga ggtgaaggcg acatggatgc aaaatcgctt tctggtcttg
     2641 acctcgtgct tggttcattt cactcatctc tgcggcgaac cgatgaccag accgagcgat
     2701 atctcgcggc gttgcgcaat ccgcatatcc aaatcctcgc ccacccgcgg ggccgtattt
     2761 acaattttcg actcggattg aaggcggatt ggccgcgggt ttttgccgaa gcggtcaggc
     2821 tcgacaaagc cttggaaatc gattgttacc cagatcgtca agacctgaat ttgaccttgc
     2881 ttaaactggc ccgctcagca ggaacgcgag tgtcgttagg cacagatgcg catcatccat
     2941 ggcagctcga attcatccag cttggccttg ctgcagcact gcgcgccagg attcccgctg
     3001 aaaggattgt taattttatg tcgattcagc agataaaggg ttggattgcg cgcctgcgcg
     3061 aacgaagccg ctgacgtttg gacccaattc caatgatatg ggattcggca aatcgtttgc
     3121 gttgcaaccg cggccagaag gggctgcgat tctctatgag aagatgaacg aaaaagctgc
     3181 aaatcgcgca gatcaaacgc cccgttcgtt ccgctgcaag gttaatgatc tgttccgcgc
     3241 tttttcgcgg aatattgcag cggcggttgg atcggcgtgg gcgttcattg ttgcaatcac
     3301 cgtcgttatt gtttgggccg cgactgggag gatatttcat ttttccgata cgtggcagct
     3361 tatcattaac accggcacga ccattgtaac ctttctgatg gtgttcctga ttcaaaacac
     3421 ccagaaccgc gacgggaaag ccattcaatt aaaactagac gagttgatcc gggcccttag
     3481 aggtgcgcgt aacaaacttg tcgatcttga agatctttct gacgaagagc tgaaggcact
     3541 ccaaaaagaa ttccagcgct tgcagaaaca atctcgagaa gccgacacgg acgtcgcgaa
     3601 aaccgaatcg cgacgttgaa tccgtagccg gtgcatccta gcgatcgcag gagaatcttt
     3661 taggacaatt ccgctgtcta aactgctggc cctgatagcc cgcggcgccg gacgttgcct
     3721 ctcggccgag tcccacgccg cgagaagggc gatctattca gcgggccttc gcgtttccgt
     3781 tatcgatttg aacccgcgcc agtcagctcg gtctccatga tggcttccgg attcacgatg
     3841 ccttcagcca gttggttgag cgcctcgttc gtttcggatt cttcgtcgag agtggtttgc
     3901 agaagctccg ctgacctgtc ctttccgagc agctgagcaa aggtgcgaac actcccgtaa
     3961 cccgcaattt cgtaatgttc gactttctgc gcagctacga tgatgccggc atcaattacg
     4021 gagtcttcgc catcttcgtg aaggatttcc gatccttcct caatgagccc tttcatggcg
     4081 cgacaggttt tacctttcgg cttctcgcca atctccccga aaacctcttc cagccgttca
     4141 acatgagatt tggtctgctc cagatgtttc tggaacgcgt cctttagttc ttctgatgaa
     4201 gcagctttcg ccattttcgg caatgctttg agcagttggt tctctgcgtt gtagaggtcc
     4261 cgcaattcat tgatgtagag tgtctttaat gtattcagct tcataccatc cctttccctt
     4321 tgttattcct gaatttggtt ttgaattttg gcgatccagt gagcactgga ccccagctct
     4381 agaaaacaac gcgcctcaac atggaattgc gcgtgtttct tttgaaaatt tttccgagtg
     4441 tgcgcgggct tcgcttttac gctcccgatt gtgttcaaaa cgtcccggag ctggccgaac
     4501 acgctttaac aggctgaaag ccagctgact ggcgcgagtt gatggaagcg tccaccactg
     4561 gctgcgctgc gaactacatc aaagaatgtt gttgaagccg ccgttgccgc ccatggaagc
     4621 gcgttcagtc gaggaaattc cggctggaaa cagctggcag tacgagccga agtgggacgg
     4681 ctttcgttgt cttgcgtttc gggatgacca aaagattcac ttacaatcaa aggctggtca
     4741 gccgctcggg cgctatttcc ccgatgtcgc cgatgctctc gccagattgc ccgagcaaca
     4801 atttgttctc gatggcgaac ttgttgttcc cgtcagcggc gtactttcat tcgatgaatt
     4861 gcaactgcgt ctgcatcccg ctacgagtcg cgttcagaaa ctggcaaaag ctcatccagc
     4921 aatgtacatc gttttcgatc tgctcgccga gaaggggcaa gtgtatttgc agcgaatcct
     4981 gcgggaacgc aggcgactcc tcaaaagatt cgctcgtttg aatttcagat ccgccaaaca
     5041 tttgcgcctt tctccggcca ccactaatca tcggactgcc agcaagtggt ttaaaaaagc
     5101 cggcggcgat cttgatggca ttatcgcgaa gcgtctggac gctgcatatg cttcgggcga
     5161 acgcaccgca gtggttaaag taaagcaaat ccgcacggtg gactgtgtcg ttggcggatt
     5221 tcgttatgcg cggggcacgc gagtcgttgg ttcccttttg ctcggacttt atggtgaaga
     5281 tgggttgctt caccatgtag gattcacgtc cgctttcaaa gcgtcagaac gccgcgcgct
     5341 aacaagaaaa tttgaagcgc tcaaaaaacc accgggattc actggcagcg cgcccggagg
     5401 tcccagccga tggagcacag atcgcaccag tgaatgggaa ccagtcatcc cacgagtcgt
     5461 ggtggaagtt gcctatgacc attttaccgg cggccgattc cgacacggca caaaatttct
     5521 acgttggcgt ctcgacaaaa agcctcgtca atgcacgatg gaccaggtgg agcatcgtga
     5581 aggcaagtcg ctcgcgcttt tatagctaca cgtgatgtta agtgggaacg gagtggagct
     5641 ggagcttcag aattaggcca attatccgac cccaggagcg tgccgtattt cttgctcgtc
     5701 gcgaaatgcc aattccctac ccacgacggg ccttaagaca ggcgtttatt ttgttttgcg
     5761 actcgaactc tttttcgaaa catcaataaa accaatcttc gtctgataag cgaatctatg
     5821 tgttatgacg aatctacagt tcaagggcag ttggaacgag gcaaagggca aacttaaaca
     5881 aaagtacggt caattgaccg atgacgacct tgccttcgcc gaggggaaag acgacgagct
     5941 tctcggccgc ctccaaaaga aactcggcaa aaccaaagaa gaactccgaa aggagattga
     6001 gagcctgtaa aggcaaaggg ataaaacaag atgcggctaa caaacaaaat caaagaagca
     6061 ggagcaaccg gatacatttt actttggctg ctagggattc caattccgat tttgctgtta
     6121 atctttctcc tgcgcggttg cacgtaacaa ttgaaataaa tcaaccggaa aatcaatcta
     6181 tgctgtggac catatttgtg attctcctcg ttctgtggct tctgggtttt ctcggccacg
     6241 tcggcggagg attaatccat ttgttgctcg tgattgcggt cgttgtccta atcattaacc
     6301 tgattcaagg cagacgatcg ctctgaccta tagccgcact tttccgtgcg gagctaatgg
     6361 gagcactatc tcctcagccc agtcggagat gaggttgcac ctgctggtta gctcactttc
     6421 gctatgattt gatgcggtcg cacacgttcg ccaggacaag cggcggagaa agaaaaaacc
     6481 gaatttcggg gcggtggcct cacggacaag aaaaacggcg ggagttgcct cccgccgttc
     6541 agtaaacgaa cgcacaagac ttatccaccg ccttgctgac cgccaccttt ttggccgccg
     6601 ccgccctgtt ggccaccgcc tccttgtttc tcgcgatcgc gatcgcgctg ttggccgccg
     6661 ccctcttggc cgccgccctg ctggccgccg ccttgctgac cgccaccgcc ttgtttgtca
     6721 cgatcacgat cgtcctgttg gccgccaccc tgctgttcgg cgcgtgtagg tctgatgggt
     6781 tcattcggat tgcgttgtgt ttccattgtt acctttcttt gttaatgatt ggtctttcgc
     6841 acttcgcgac gagacctttg ctgtttctct gaaatcgcct cggaacgggc gtttggattg
     6901 gagaattttt acaagcgacc ggcccgacgt gttgaagatc tgcggccaat tggttcagca
     6961 tggattcagc tcttgaatcc tgtccgatag ttctgagaca agtcgctcgc ggtttcccag
     7021 gcgaacctca attagggtca catcaggacg cgatttgatc gcgtcggtaa acggtaggga
     7081 tcgagcgtag attgtcgcga ttaccgggac ttcactgtcg agcacctcag ttactgagtc
     7141 gcgaaaaatc gcggagcgga tctccatcgg accgatttca tcgatcacca caagccgccg
     7201 cgtccggacc gcccgacgga ccgcgccgac gccgattctt tccagcgccg aaagatcgag
     7261 tccgtatttt cctaggcgat cgggcgtttt gaaatcgaca tgggcgaaca cagcctcttt
     7321 accaccgagc gtaacgacct tgaatccgac acgcgcgcca gcctcgcgga tttcctcagt
     7381 gtaaaagcca ccagcgcgtc gcggcagatt atttacaacc cgcttgatca atgtcgtctt
     7441 tccacatccg ggacgcccgg tgagcaaaac ttttttggtc ataaggcaac gtgaggcagg
     7501 caatcatgcc tgccccgctt aagtgacgat gtgttcttgc cggcgcagac agggattgtc
     7561 tgcgtcaccc taccgaaaag accaccggac acatctgcga cgattccttc taaaggagga
     7621 caaatagcca tggcaaccaa tcacgacatc atcaatgaac tgagaaaatc atacgcgatg
     7681 gaacttgaga cggtggagaa ttatctggca aacgcgatcg atctcgatgg cgtgcgcgca
     7741 gaggaaatca agaaggcgct tcttcgcgat attgaggaag agctcggtca cgctcggaag
     7801 ctgggcaacc ggatcaaggt gctcgaaggc cgcgttccgg gctcactcga tcttgggcgc
     7861 gggcagcgct atttgcagcc gccggaggat agcacggatc taatcgcagt gattcgcggc
     7921 gtgatccgcg cagaggaaga ggcgatcgat gaatacaaaa aaattatcaa gatgtgtgac
     7981 ccgatcgatc tggtaacgca ggacctaatt ctcgacatca ctgcgcagga acaagcgcac
     8041 cgccgccaat tcattgggta cctctacgag tacgaacgcg gcgaagccaa gcgactcaca
     8101 gccgcagcgg cgtagcggag gatagacatc ttgtctgtct cggcagcatg cttccagcat
     8161 gtcgaggatc ttaagctgaa tcacggtagg caagatgccc tcccgccgtc aggctggaag
     8221 cctaagttcc gaacgcgcgc ttcttctggc agcgcgcgca aaaccacgcc gtgcggccgc
     8281 caatctgtgc cgatctcaga ttgcgtccgc atcgcgggca tcttccgccg tttccgcgat
     8341 gtgggagcag ccacgatttc ggcgttcgat cgacatcggc tttggattcg atcgcattct
     8401 ccaagatgta gcgcatggcg cggaaaagct tcgcgattgc cttgtcgttg agagcagaaa
     8461 tttccgtcgc cggatgcatg tgggcgcgga aaagaatttc gtcggcataa atattgccga
     8521 tccctgcgat cagccgctgg ttcagcagga tcgatttcac ggcgccgcga tgttttgcga
     8581 acaagccttg gaattcttta agctcaacat cgagcgcatc agggccgagg gcgcgctttt
     8641 tcagaaacgc atcgacatct tcgatcaatc cgacctcgcc gaattttcgc tgatcgtcga
     8701 aagcgagcga gtagtccttt tcgaaagcaa acagtactcg cgtgtgcggc ggcgcttttt
     8761 cttcgccttt gaaataacgc aacgaaccgg tcatgccgaa gtgcaggcgc agccacacgt
     8821 cgctatccat gcgcacgaac agatgtttgc cataacgccg actcgattcg aagccgcgcc
     8881 ctttcaatcg gcgtgcaagc tcacgcgctg agatgccctt caagacatat gcgctccgca
     8941 catcgacatc ggcgatgcgc tgatgcagcg acgtagcgtc gaggtatcgc ttgaaagtct
     9001 cgacatcagg cagctctggc atcgggttag cgtgacgcag gcaatcctgc ctgcgagcat
     9061 tggatgcgca agtaagcgta gggatacagg caggattgcc tgccccacac gaatactaaa
     9121 tgtatgacgg cgaagaaatt tgtgctcggc ttcgacaagg tttcatccaa agatacgaac
     9181 cgggtcgggg gcaaaaacgc cgcgcttggc gagatgattc gcacgctcaa acgcaaaggc
     9241 gtgcatgtgc cgaatggttt cgcgacgacg gccgacgcct tccggctttt cgtccgcgaa
     9301 aacaagatcg ataagaaaat ccgcgcggag attcagcggc taaagaaagg ttcgaagtcg
     9361 ctggccaccg cgggcaaagc actgcgctcg ctttttttga aaggcaaatt tcccgagcat
     9421 gtggaggagc agattcggga agcatatcgc gagctttgcg agcggtacaa aaagcgcgca
     9481 ctggatgtcg cagtccgcag cagtgctacc gccgaggacc tgcccgaggc gagtttcgcc
     9541 ggtcagcagg aaacttttct caacatctca ggcgaggacg gtgtcgtcga tgcgtgccgc
     9601 aaatgttacg cttcgctttt cactgatcgc gcgatcagct atcgcgagga gaagggtttc
     9661 gatcatctcg aaatcgcgct ctcggccggt gtgcaaaaaa tggtgcgcgc cgacaaagcg
     9721 ggcgcgggcg tgatgttttc gatcgacacc gagagcggct ttccgaatgt cgtgcggatc
     9781 agcgccgcgt ggggtttggg cgaggtggtc gtcaaaggcg cagcgaatcc cgacgaatac
     9841 atgattttca agccgttgct gggcaagaga aacacgcagc cgatcctgga aaaactgctc
     9901 ggcgacaaga aacgcaaagt cgtttacgcg tccggcagcc gccggagcgg ccacatgacg
     9961 cgcacggtca acaccacgaa gaaagaacgg gaatctttcg tgctcgacga cggtgagatc
    10021 ctgcgcttgg cgaaatgggc gtgcgcgatc gagaagcatt accgcaaacc gatggacatc
    10081 gaatgggcgc aagagggtga taccggggaa attttcatcc tccaggcgcg gcccgagacg
    10141 gtccaggcac tgaaagaagc aggctcgctc acgacttatc gattgaaggg aaaaggcaag
    10201 cgactgttct ccggtttgag tatcggcgag gcgattgccg cggcgaaggt gtgtcgcatc
    10261 caaagtgcgc gcgatctcgg caagttcaag gagggcagca ttctcgtcac cgaaatgact
    10321 gatcccgact ggggcccgat tttcaaaaag actaaaggca tcatcaccga cctcggcggg
    10381 cgcacttgtc acgcggcgat cgtgagtcgc gaacttggca ttccggcgat tgtcggtacg
    10441 ggcgaggcga caaaaactct gcgcgacggc caggaaataa cgctctcgtg cgccgagggt
    10501 gatgaaggct ttgtttacga agggttgctc gatttcgccc aggaaaaagt gagtcttaaa
    10561 aacctgccag agacgcgaac gccgatcatg ctcaacatcg ccagcccggc cggcgcattc
    10621 cgctggtgga agttaccgtg cgacggcatc ggtctggcgc gcattgaatt cattatcgcc
    10681 aatcgcattc agattcatcc gatggcgctg gcgcatttcg atgagctgaa ggaccgcgcc
    10741 gcacgcaagc gaatccaaat cctgactcac ggttacaaga acaagggcga ttatttcatc
    10801 gacaagctcg cgcaagggat cgcccgcatc gctgccgtgc ggcatccgca tccggtgatt
    10861 gtgcggctga gcgatttcaa gacgaacgaa tatgccgcgc tcatcggcgg cgaacaattc
    10921 gagccacccg agcagaatcc gatgctcggt ctgcgcggcg catcgcgcta ttaccacgag
    10981 cgatatcgcg atgccttccg gctcgagtgc gaagcgatca agcgcgtgcg cggcgacatc
    11041 gggctgaaaa atgtgatcgt gatggtgccg ttctgtcgaa cgctcggcga ggccgacaag
    11101 gtcctgcacg ccctcgccga gaacggtctg gtgcgcggcc gcggcgggct ggagatttac
    11161 atgatgtgcg agataccgtc gaatgcggtg ctcgccgaaa aattcgccga gcgcttcgac
    11221 ggtttttcca tcggcagcaa cgatctcact cagttcgtgc tcggagtcga ccgcgattct
    11281 gccgcgttag ccgaactgtt tgacgaacgg aacgaggcgg tgaaaaacgt tgtccacgac
    11341 gtgattcgcc gcgcacataa aacagattgc aaagtcggca tctgcggcga agcgcccagc
    11401 aattatcccg agtttgccgc gtttcttgtt gagtgcggca tcgactccat ctcgatcaat
    11461 ccggaccgct tcgttgagac caaaagaatc gtagcaaggg cggagcaaaa ccgctgatgt
    11521 ccgatcaagt cgaaaagttc atcaggcttt tgaaagaatc gccagccggc gctgtcttta
    11581 atccctggtg gcaagttgat gaagaaaacg acgcgggtcg gaatgcgccc gccatccggc
    11641 ggaaacagtt gagcgcctat cttagcaagc gtctcaaaaa agcgcgactc gcagtaattg
    11701 gcgaagcgct tggttaccgc ggcggtcatt tcagcggaat tcccatgacc tccgagcgca
    11761 ttctgcttgg aaaaaagaaa ga
//
LOCUS       DASQUQ010000216         4993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_49998, whole genome shotgun sequence.
ACCESSION   DASQUQ010000216 DASQUQ010000000
VERSION     DASQUQ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49998"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VE486_06150"
     CDS             complement(<1..180)
                     /locus_tag="VE486_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38704.1"
                     /translation="MNETLFSQIQRLFERTYAQVGINLEDCLIDQKRCAQLSILAGKS
                     ARELSELARTFLRRAG"
     gene            complement(177..878)
                     /locus_tag="VE486_06155"
     CDS             complement(177..878)
                     /locus_tag="VE486_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38705.1"
                     /translation="MIRANCRARFTAADFDFIVRTLARSQTDQVSLVDLLSDVETRDS
                     ILDHPRLVDAILNSCGHLRISSQFYFYVLARHVLQQGGIGDRKLCDYIASLLETFSRA
                     SRLQVSDQADHLAEQYISDMLIALTRATPEQAFLLRAHIGNYSLFISGIFHENTQRRS
                     LRGGPDIEFYEQVGRTNYQLVASHAVARRCELDDVFEGLADRFREVRLALNQLSDQLL
                     NLDDPAAAGHPSLSL"
     gene            1171..1596
                     /locus_tag="VE486_06160"
     CDS             1171..1596
                     /locus_tag="VE486_06160"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZA38706.1"
                     /translation="MADTHTGGCYCGAVELEASGEPLDMGYCHCEACRRYSTAPVSVF
                     TLWKPENVKITKGEQLLGKFKSSEMSNRRYCTECGGHVMIDHPTLGLADIRVPLSGVT
                     FKPTVHLNYEERVLPIRDGLPKLKDFPAAIGGSGETMPE"
     gene            complement(1720..2166)
                     /locus_tag="VE486_06165"
     CDS             complement(1720..2166)
                     /locus_tag="VE486_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38707.1"
                     /translation="MNRWSFLIVSLLFSTGLRMADATSKSEFDAALKQSNRDRETPSG
                     KRYTRQFEDKVFLAVASKAMQTCLSRSRDTVEPAMLVFLVSADGKVTRVLSTPGIEYG
                     KCVVSQLRLPISVPRPPRDNFAVALGIANHTHAQSKAPPGKPVRAR"
     gene            complement(2445..3473)
                     /locus_tag="VE486_06170"
     CDS             complement(2445..3473)
                     /locus_tag="VE486_06170"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HZA38708.1"
                     /translation="MSSAAAELYRKAFVLDCNALAGIGFPISDDDPVGVTKLILGSGV
                     NVVKATVGGAQGKFEEAVAAIAKAELLMEKQPDAFFKVQNVADFDRAKKEGKLGVIYS
                     FESPNMLEDKIDRIDLFRGLGVRVMQLTYNRRTPLGCGCLDGETDGLTELGRNAIGRM
                     NEIGVALDLSHSNTKTTADGIAASKKPVLITHAGCRAVHTHPRNKEDRELKALANKGG
                     VMGIYMLPYLTESPKQPMLEDYLQHLDHAVKVCGEDHVGIGSDIPFLHVGEAGLADMK
                     NDAEQRKTAGIAAPGEDRPTYIPDLNTERKLEFVTDALLKRGYKSGAGEKILGGNFKR
                     VFGEIWSA"
     gene            3561..3812
                     /locus_tag="VE486_06175"
     CDS             3561..3812
                     /locus_tag="VE486_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38709.1"
                     /translation="MSSSHVSTGISAELFDKIVVFLVRADPEPHDEITVTTCEGAVMV
                     ADSNRPNVSDKWFELHGGMKWIALPNVELVPGKTLHVRR"
     gene            complement(3854..4054)
                     /locus_tag="VE486_06180"
     CDS             complement(3854..4054)
                     /locus_tag="VE486_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38710.1"
                     /translation="MSLAEIQSAIEQLSFEERAKLAAWLHGWKDDDWDEQMKRDVASG
                     KFEDVLREVDDDIRAGRLRDMP"
     gene            complement(4240..4584)
                     /locus_tag="VE486_06185"
     CDS             complement(4240..4584)
                     /locus_tag="VE486_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38711.1"
                     /translation="MKWITSAAAAAMALLALSGCDKKTSDDLKEKAEDAKQTVEAKTK
                     EAKEAAEKQIEKAKEAAKNAQPKLEEMADKAKDATQNAIEKTKDAADSAAEKLKASAN
                     ASPAETSPTPTP"
BASE COUNT         1095 a         1427 c         1354 g         1117 t
ORIGIN      
        1 gccggcgcgg cgcagaaatg tgcgcgcaag ttcgcttaat tcccgggccg attttcccgc
       61 caggatcgac aattgcgcgc aacgcttctg gtcaatcagg caatcttcca gattgatccc
      121 aacctgtgca tacgttcgct caaataacct ctggatttgg ctgaagagcg tctcgttcac
      181 aaggagagcg atgggtggcc ggcagctgcc ggatcatcca gattaagcag ttgatccgaa
      241 agctgattga gcgcgagccg cacttcgcgg aagcgatcgg ctaatccttc gaacacatcg
      301 tctagttcgc agcggcgagc gaccgcgtgt gaggcgacaa gttgatagtt tgtccggcca
      361 acctgttcat agaactcgat atccggtccg ccgcgcaaac tgcgccgctg ggtgttctcg
      421 tgaaagatgc cgctgatgaa aagtgagtag ttaccgatat gggcccggag caaaaatgct
      481 tgctcagggg tcgcgcgggt cagcgcgatc aacatgtcgg aaatatattg ctcggcgaga
      541 tgatcggctt ggtcagaaac ctgcaaccgg ctcgcgcgcg agaatgtttc aagcaacgac
      601 gcaatgtaat cgcaaagctt gcggtcgccg atcccgcctt gctgcaaaac gtgacgggcc
      661 agaacgtaga aataaaattg agacgatatc cgcagatggc cgcagctatt taagatcgca
      721 tcaaccaatc gcgggtgatc gagaatggaa tcgcgtgttt caacatcgct caaaagatcg
      781 acgagagaaa cctgatcggt ttgcgagcgc gcgagcgtgc gcacgatgaa gtcgaaatcc
      841 gccgccgtaa accgggctcg acaattcgcc ctgatcatcg ggaactcatc atgcgcaata
      901 cgcgcgctcg tggcaagcgc gatttcaact gctgagatat caagcgaatc aggaaaccag
      961 gaatcgagga gctgggtgcg acgacatgca cgggacagcc gcgaggtagc ggagagcagt
     1021 tcgggggaac tgcgaatgaa aacaggaatt caaataagat tcacaggatt cagcggattg
     1081 aagaagccgt gactaatttg acgcgccact acgaaaagga accggattaa ttcttttaat
     1141 ctatgtcgct cctagcataa catctacgac atggctgaca cacatacagg cggatgttac
     1201 tgcggagcgg tggaactcga ggcaagcggt gaacccttgg atatgggtta ttgtcattgt
     1261 gaagcttgtc ggcgttattc caccgcaccg gtaagcgtgt tcacgttgtg gaaaccggag
     1321 aacgtaaaga tcacaaaagg cgagcaattg ctcgggaaat tcaaaagcag cgaaatgagc
     1381 aaccgccgat attgcacgga gtgcggcggt cacgtgatga tcgatcaccc caccctcggc
     1441 ctggccgaca ttcgcgttcc tcttagcggc gtgacgttca agccaacggt gcacctcaat
     1501 tatgaggaga gggttcttcc gatccgagac ggcttaccca agctaaagga ttttccggct
     1561 gccattggtg gatcgggcga gaccatgccc gaataacacg gtccaatctc tcaattaaag
     1621 cgaagcgttc tctcaacgat cagcttcggc actgacggcc tgccgaagct aaaaaaatgt
     1681 ccccactgca atcggcggct ctggcgaaac gctaccggac tagcgcgctc gaacgggttt
     1741 gcctggcggt gccttgcttt gcgcgtgagt atgattggcg atgccgagcg ccacggcgaa
     1801 gttatcgcgc gggggacgcg gcactgagat cggcagccgt agctgcgaaa cgacgcattt
     1861 gccgtactca atgccgggcg tcgatagaac gcgcgtaacc ttgccgtcgg ccgagacgag
     1921 aaataccagc atcgccggct cgaccgtgtc gcggctccgg ctgaggcagg tctgcattgc
     1981 tttcgacgcc accgcgagaa acaccttgtc ttcgaactgg cgcgtgtagc gctttccgga
     2041 aggtgtctct ctatcccgat tcgattgttt gagtgcggcg tcgaactccg acttcgaggt
     2101 cgcgtcggcc atccgcaaac cagtcgaaaa aagtagcgag acaatgagaa aactccagcg
     2161 gttcacgacg aagctagatt aaacgatctc tcgtacgtcc ggtcaacgtg cgcttcaaag
     2221 cgaaacagcc gctcctttcg agctcggctg tttgttactg ttgtttccgg ctgcgaattt
     2281 aatcaggaat ccaggaaata aagccaatcc tgctttgatg cgcgctcccg cgctccgctc
     2341 cgcttacctc gcggctgttc cgtccatctc ggaaatccct ttcctcgatt cctgctttcc
     2401 taattcaact ctcctggctt ccagatttca cctcagaaaa acgattatgc cgaccagatc
     2461 tcgccgaaga cgcgcttgaa atttccgccg aggattttct ccccggcgcc gcttttgtag
     2521 ccgcgcttta acaacgcatc ggtgacgaac tccagctttc gctcggtgtt caggtccgga
     2581 atgtaggtcg gccgatcttc gccgggcgcg gcgatgccgg ccgttttacg ttgctcggcg
     2641 tcattcttca tatcggccag tccggcttcg ccgacatgga gaaacgggat atcactgcca
     2701 atgccgacgt ggtcctcgcc gcaaaccttc acggcgtgat caagatgctg gagataatcc
     2761 tcaagcatcg gctgcttcgg cgactcggtc agatacggca gcatgtaaat ccccatcacg
     2821 ccgcctttgt tggccaacgc tttcagctcg cgatcttcct tgttccgcgg atgcgtgtgc
     2881 acggcgcgac aacccgcatg ggtgatcaac accggtttct tcgacgccgc aatgccatcg
     2941 gccgtagtct tcgtgttcga atgactgaga tcgagcgcga caccgatctc attcatcctg
     3001 ccgatggcgt tgcgaccgag ctcggtcagg ccgtccgtct cgccgtccaa acaaccgcag
     3061 cccagcggtg tccggcgatt gtaggtaagc tgcatcaccc gcaccccgag cccgcggaag
     3121 agatcgatcc ggtcgatctt gtcctcgagc atgttcggcg attcgaaaga gtaaatgacg
     3181 ccgagttttc cttccttctt cgcgcgatcg aaatccgcca cgttctgcac cttgaaaaac
     3241 gcgtccggct gtttctccat caacagctcc gccttcgcga tcgccgccac cgcttcctcg
     3301 aactttccct gcgcaccgcc gaccgtcgcc ttcaccacgt tcacacccga gccgagaatg
     3361 agcttcgtca ccccgaccgg atcatcatcc gagatcggaa atccgattcc agccagcgca
     3421 ttgcagtcga gcacgaacgc cttgcggtaa agctccgccg ccgccgacga caccggaacc
     3481 ggtgtagcac tatcagctga ccaaagtaag ccaggcctga ttgcggcgag cgaagccgcc
     3541 gctccgacca ttttcgcaaa ttgtcttcga gtcatgtctc aacaggcatt tcagcggagc
     3601 tgtttgataa aattgttgta ttcctcgtgc gtgccgatcc agaaccacac gacgagatca
     3661 cggtcacgac gtgcgagggc gcggtaatgg tcgccgattc gaacagacca aacgtttccg
     3721 acaagtggtt tgaactgcat ggagggatga aatggatcgc gttgccaaac gtggaacttg
     3781 tcccgggcaa gacactgcac gtgcgccggt aatcggttga actcgttcca gaatcgccga
     3841 tgcgttctgg acttcacggc atatcgcgga gtcggccagc gcggatgtca tcatcaactt
     3901 cgcgcaagac atcctcgaac ttaccgctcg cgacgtcacg tttcatttgc tcatcccagt
     3961 cgtcgtcctt ccaaccgtgc aaccacgccg caagcttggc ccgctcttcg aaggagagct
     4021 gttcaatcgc gctctgaatc tcagccaagc tcattgctca actttagcgc gccgccgtca
     4081 tttcagcaat tgcttgccac gccatagcct cggctaacgg agcgactggc gtgagcgaca
     4141 cgtatgaatt gaagactaac gagtcaggca attgacgtag cgtgtgctcc aataacagcg
     4201 tcagcgacat ccgcgtgatc cgcggttagg ccgaggacgt tacggcgtcg gtgtcgggct
     4261 tgtctcggca ggcgacgcgt tagccgaggc tttcagcttt tcagcggcag aatcagcggc
     4321 gtccttagtc ttctcgatcg cattctgcgt ggcgtccttt gccttatcgg ccatctcctc
     4381 gagttttggc tgggcatttt ttgccgcttc ctttgctttc tctatttgtt tttcggcggc
     4441 ttctttcgcc tctttcgttt tggcttcgac cgtttgtttg gcgtcctcgg ccttctcctt
     4501 gaggtcgtca gaagtttttt tatcgcagcc cgacaacgcg agcaatgcca tggccgcagc
     4561 ggccgcagaa gttatccatt tcatagagta gttcctgttc ggttgttcat gaacagtgcc
     4621 tcgtctttgt gtcagattca aagcttttgc acagaattgc ctggatcgcc gcgacctcga
     4681 ggggacgatc gattctgcgg aaacggcttt ctcaaatgtc gaatgtttag atttgacccc
     4741 gattccccga ttcaacgcgc attcaaatcg gatgagagta gattcggact gcaccggcaa
     4801 acagtgccac gcgcctgaaa tttttttcga aaccgagagc agttgtgtcg aaagtgagga
     4861 ctgaccccaa tcaccaagtt tggtgttcgt tgatccaaaa tccaatgagg tcggccttac
     4921 actcgaagac gatgacctga ttcgggtgcg cgggcaattg gacctgagcg gcgacaaggt
     4981 agtcgttata gcg
//
LOCUS       DASQUQ010000217         6699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_328977, whole genome shotgun sequence.
ACCESSION   DASQUQ010000217 DASQUQ010000000
VERSION     DASQUQ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328977"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VE486_06190"
     CDS             <1..210
                     /locus_tag="VE486_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38712.1"
                     /translation="GHIYKGHRLAGFLWMAGAIPVGIFVLLAAIASAGFGIGLFFFYL
                     IAVMLHAYAVDDRIVPPREDEGEEY"
     gene            complement(254..1303)
                     /locus_tag="VE486_06195"
     CDS             complement(254..1303)
                     /locus_tag="VE486_06195"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZA38713.1"
                     /translation="MRTIIVTGSAGLIGSETVKRFAKDGARLVGIDNDMRAEFFGAEA
                     STKKTRDDLIANSPGYQHHDLDIRDASAVMELFKNQGGKIDAVVHTASQPSHDWAARD
                     PQTDFTVNANGTLNLLEAARKFCPEAPFIFTSTNKVYGDTPNRLPLRELEKRWEIASG
                     HEYEPGISETMSIDYTKHSLFGASKVAADVLVQEYGRYFEMPTVSFRGGCLTGPAHAG
                     TELHGFLSYLMICTVSGRPYRVFGYKGKQVRDNIHSFDLVEAFAEFIRAPRVGEVYNI
                     GGSRHSNCSMLEAIEMCEEISGKKLNWTYDETNRAGDHIWWISDVRKFQGHYPQWKFR
                     YGMREILEEIHRAVG"
     gene            complement(1359..2054)
                     /gene="pgl"
                     /locus_tag="VE486_06200"
     CDS             complement(1359..2054)
                     /gene="pgl"
                     /locus_tag="VE486_06200"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZA38714.1"
                     /translation="MGRRIIRTHNFAADVADFIVDQAHKAIGQRNEFRIALSGGNTPR
                     PTYEKLAGIRRDLPWELVRITFGDERCVPPDDPQSNFKMVREALFVPAAVPENSIMRM
                     RGEIDPRIAAQEYEDGLDVLATQRGEPIYRHDLILLGLGEDGHTASLFPGTAGLEETT
                     RRVIANFVPKFNSWRLTFTFPLINHARHICFLVDATKHKDLIERVIEGDPQFPASRVN
                     PTAGELTWILGQS"
     gene            complement(2067..2570)
                     /gene="msrA"
                     /locus_tag="VE486_06205"
     CDS             complement(2067..2570)
                     /gene="msrA"
                     /locus_tag="VE486_06205"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZA38715.1"
                     /translation="MSNRSIVTGMKTEKATFGAGCFWGVEETFRKLKGVISTAVGYAG
                     GTKDNPSYEDVCTDETGHAEVVEVEFDPSQITYNELLEVFWSNHNPTTLNRQGPDVGK
                     QYRSVIFYHSPEQKADAESSKAELEKRGRFNRPIVTQIEPAPRFWRAEEYHQRYLEKR
                     GQTHCAI"
     gene            2686..3951
                     /locus_tag="VE486_06210"
     CDS             2686..3951
                     /locus_tag="VE486_06210"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZA38716.1"
                     /translation="MNSSATTLEQTRSLLPVRLLNGCGALLEKTRIPHTPVLALDLIE
                     TAMRRCGLSDFGRGDFLEALSRLLESCQREARLNVIGKIALRADPIRILCSRLFLERD
                     RQLYPGIARQEIREPLFIVGLPRSGTTLLHTLLAADPEHRVPLTWEVMTPSPPTNDNE
                     RQRIRRATQSCNCLNWLAPTFRHIHPLGAQLPQECVSLMTPTFLSDQFDTMYYVPSYR
                     AWFLRQDLLPAYEYHRRFLQHLQFRRSAPRWVLKAPTHMFALPTLLSVYPDALFVQAH
                     RAPLEAMASVSSLITILRRVFSKAVDPFVVFREAIQYWSETLDKFLQERDRLEPRRIC
                     DLAYSEIRCHPIAAVRRIYEHFGWSLTRETEQRMRVVLANQPQDQHGFHRYNLSQFGL
                     QEGAGAEAFAAYCERFGLSTHVPIVADAG"
     gene            4091..4696
                     /gene="pgsA"
                     /locus_tag="VE486_06215"
     CDS             4091..4696
                     /gene="pgsA"
                     /locus_tag="VE486_06215"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZA38717.1"
                     /translation="MTTANKITVVRILMIPAFVTMAIYYGQSIQRGQPLEWQRFTAIA
                     IFLIAAISDGLDGYVARRYNQRSSLGVILDPIADKGLLLSGIITLSISNWSEIDPDYG
                     RFPAWFPVLVISRDAVILVGAAVLYLLNGKVQVRPRWSGKVATVCQMIAIGWVMLQFH
                     FIPLFYIVIGAGFFTLLSGVVYVMDGVRQLQAEGHAHATRH"
     gene            4712..6097
                     /gene="der"
                     /locus_tag="VE486_06220"
     CDS             4712..6097
                     /gene="der"
                     /locus_tag="VE486_06220"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZA38718.1"
                     /translation="MNGAANNLPNVAIVGRPNVGKSALFNRLIGRRIAIVHDQPGITR
                     DRISAVCTRGTQPFVVWDTGGIAGAGESELTAQVRLAVQQAVHDSEVLLFLVDAKTGL
                     SPLDQELARMLRKSHKPVILVINKIDDARQENLAADFASLGFQHSHSISAEHNRGISE
                     LLEPIDQLLPSSVAATEIKHQTSNIKPPAAIAIVGRPNVGKSSLINSIVRSERAIVSE
                     LPGTTRDSVDISYQRDGQEFLFIDTAGIRRRGKHSSSVEVFSVMRAERSIRRADLCIL
                     IVDLTMGVTAQDKRIAGLIQKARKAAIVVLNKWDLIRLKRREKQTIARLVDETRARIF
                     FLDYTPVLVASALTGQNIEKLFALIENVRRAAQQRIGTGVLNRLLRAAFAANPPPMIS
                     GRRLKLLYAAQASGKQERPLQPPEFVLFVNEPRLLSETYRRYLEARIRKAQPYPGLPI
                     ILTLRPRSQNS"
     gene            6150..>6699
                     /locus_tag="VE486_06225"
     CDS             6150..>6699
                     /locus_tag="VE486_06225"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZA38719.1"
                     /translation="MKSTQEITQEVRELGQWIPPLRQQVGHVVVGQKYLVDRLLLGLL
                     ANGHILLEGVPGLAKTLTVKTMAACIQTGFQRLQFTPDLLPADLIGTLIYNPRTGEFT
                     TKLGPIFSNLILADEINRAPAKVQSALLEAMQERQVTIGEQTYPLSDPFLVLATQNPI
                     EQEGTYQLPEAQLDRFMFKLNIG"
BASE COUNT         1407 a         1949 c         1747 g         1596 t
ORIGIN      
        1 ggccatattt acaagggaca cagactggca ggctttttgt ggatggctgg cgctattccg
       61 gtagggattt ttgtgcttct ggctgcaatt gcatctgcag gatttgggat tggcctcttc
      121 ttcttctacc tgatcgcggt aatgcttcac gcctatgcag ttgatgatcg tatcgtgcca
      181 ccgcgcgaag acgagggcga agaatactaa ggcgtgagta ataaatgcga cgctcataga
      241 gcgccgctac aggtcatccg accgcgcgat gaatttcttc cagaatttcc cgcattccgt
      301 agcgaaattt ccattgcgga taatgcccct gaaatttccg cacgtcgctg atccaccaga
      361 tatggtcgcc ggcacggttt gtctcgtcat aggtccagtt taattttttt ccgctgattt
      421 cttcgcacat ctcgatcgcc tccagcatcg aacagttgct gtgtcggctc ccgccgatgt
      481 tatagacttc cccgacgcgc ggcgcgcgaa tgaattccgc aaacgcttcg accagatcga
      541 agctgtggat gttgtcgcga acctgtttgc ctttgtagcc gaagacgcga taaggccgtc
      601 cgcttaccgt gcagatcata agataggaaa gaaagccatg cagctccgtg ccggcgtgtg
      661 ctggcccagt gaggcagccg ccgcgaaaag agacggttgg catttcgaaa taacgtccgt
      721 attcctgcac cagcacgtct gccgcgacct ttgaagcgcc aaaaagcgaa tgtttcgtgt
      781 aatcgatgct catcgtttcg gaaatgcccg gctcgtattc atggcccgac gcgatttccc
      841 agcgtttctc cagctcgcgc agaggcagcc gattcggggt gtcgccatag actttgtttg
      901 ttgatgtaaa aataaatggc gcctcgggac aaaactttct cgccgcttcc agcagattca
      961 gcgtgccatt ggcgttgacg gtgaaatccg tttgcggatc acgagccgcc caatcatgtg
     1021 atggttgcga ggccgtgtgc acgactgcgt caatcttgcc gccctggttt ttgaataact
     1081 ccatcaccgc ggaagcatcc cggatatcca gatcgtgatg ttggtaaccg ggactatttg
     1141 cgatcagatc atcccgggtc tttttcgttg aagcttccgc gccaaaaaat tccgcgcgca
     1201 tatcgttatc gatcccaacc agtcgcgctc catcttttgc gaatcgcttt acggtttccg
     1261 aaccgatcaa tcccgccgag cctgtaacga tgattgtgcg catgttttac cgtagcacag
     1321 gcaccttgcc cgtcgtcccc acagcgaggc tggccgtgct acgattgccc gagaatccat
     1381 gtcaattctc cggcagtcgg attcactcgt gaagccggaa attgtggatc gccttcgatt
     1441 acgcgctcga tcaagtcctt gtgtttggtt gcatccacca gaaagcaaat gtgccgcgca
     1501 tggttaatga gcggaaaagt gaatgtcaat cgccaggaat taaattttgg gacaaaattc
     1561 gcaatcactc gtcgtgtcgt ttcttcaaga ccagcggtac ccggaaacag ggacgcggta
     1621 tggccatcct cgcccaggcc taacagaatc aagtcgtgcc gataaatggg ttcgccccgc
     1681 tgggtggcca aaacatcaag accatcttcg tattcctgcg ccgcgattcg cggatcgatt
     1741 tcaccgcgca tgcgcatgat cgaattttcc ggaacagcag cgggcacgaa gagagcttcg
     1801 cggaccatct tgaaattgct ctgcggatca tccggtggga cgcaacgctc gtcgccgaaa
     1861 gtgattcgaa ccagctccca gggcagatca cgcctgatcc cggcgagctt ctcgtaggtc
     1921 ggccgcggag tatttccccc agagagcgcg atccgaaatt cattgcgctg gccaatcgct
     1981 ttgtgcgctt gatccacaat aaaatccgct acgtcggcgg caaaattatg cgttctgatt
     2041 atccgccggc ccatggacgc ttaaaatcaa atcgcgcagt gcgtttgtcc gcgcttttcg
     2101 aggtagcgct gatgatattc ttcggctcgc cagaaccttg gcgccggctc gatttgcgtc
     2161 acgattggcc gattgaaacg tcccctcttc tccagctcgg ctttcgacga ttcggcgtcg
     2221 gctttttgct caggcgaatg ataaaagatc acggaacgat attgttttcc aacatcgggc
     2281 ccctgccgat tcaacgtcgt gggattgtgg ttcgaccaaa aaacctccag cagttcgttg
     2341 taggtgattt gtgagggatc gaactcgact tccaccactt ctgcgtgacc ggtttcatcc
     2401 gtgcaaacat cttcatagga tggattatcc ttcgttccgc ccgcgtaccc gaccgctgtc
     2461 gagatcacgc ctttcaattt tcggaaagtt tcttccacgc cccagaaaca acctgcgcca
     2521 aaggtggctt tctccgtttt catacccgtc acgatggaac gattgctcat ggggtgcaac
     2581 cgtcccgttt gttgcttgac agttgccgcg cgccacattg gcattgccct aatgacagcc
     2641 agccgttcca aaatctgaca aaggctcgac aagctctttt atcgaatgaa ttcctctgcc
     2701 actaccctcg agcagactcg ctctctgctg ccggtccggc ttctcaacgg ctgtggagca
     2761 ctgctggaga aaactcgaat cccgcacacc ccggtccttg cgcttgactt gattgagact
     2821 gcgatgcgcc gctgcggtct cagcgatttt ggcagaggcg attttctcga agctctctct
     2881 cgtttactcg agtcctgcca gcgcgaggct cggcttaacg tgatcggaaa gattgcgctc
     2941 cgcgccgatc cgatacggat actgtgctcg cgtttgtttt tggaacggga tcgacaactt
     3001 tatcctggca ttgcgcggca ggaaatccgc gaaccgctct ttatcgtcgg tcttccccgg
     3061 agcggcacca ccctcttgca caccttgctc gctgccgatc ctgagcatcg agttccgctg
     3121 acctgggaag taatgacgcc atccccgccg accaacgata atgaacggca acgaatacgg
     3181 cgtgcaaccc agagttgtaa ctgcctgaac tggctggcgc ccacgtttcg ccacatccat
     3241 cccctgggtg cccagctccc gcaagaatgc gtcagcctca tgactcccac ctttttgagc
     3301 gaccagtttg atacgatgta ttacgtgccg tcgtatcgag catggtttct gcgtcaggat
     3361 ttgttgcccg catacgaata tcaccggcgc ttccttcaac acctgcaatt ccggcggagc
     3421 gcgccccgtt gggtgctgaa ggcgcctact catatgtttg cgttgccgac gctactttcc
     3481 gtttatcccg atgcgctttt tgtccaggca catcgagcgc ccctcgaggc aatggcttct
     3541 gtatccagct tgatcacaat cctccgtcgc gtgtttagca aggcggtcga tccgttcgtc
     3601 gtcttccggg aggcaattca atactggtcg gaaacgctgg ataaattttt gcaggaacgc
     3661 gatcgcttgg agccgcgccg catttgcgac ctggcctaca gtgagatccg ttgccatccc
     3721 atcgctgccg ttcgccgcat ctatgagcat ttcggctggt cgctgacgcg agagacggag
     3781 cagcgaatgc gcgtggtact cgcaaaccag ccgcaggatc agcatggttt tcatcgctat
     3841 aacctctcgc agtttgggct ccaggaaggc gcgggcgcgg aagcctttgc ggcctattgc
     3901 gaacgattcg gtttatccac gcacgttccg atcgtcgcgg atgctggata gctgcgtaac
     3961 aacgccatcc tggctgttcc gtagcgcggg tgttctgcat gcctcagcat cgcaggctgg
     4021 aagcccgcag ggtgagtgag ctggcgcgag cgaatcaaac gccctacaat tgttttcgga
     4081 tcgcctcatc atgacgactg ctaacaagat taccgttgtt cggattttga tgatccctgc
     4141 tttcgtcacg atggccatct attacggcca aagtattcag cgtggtcagc cgctcgaatg
     4201 gcagcgcttt actgccatcg ccattttcct gatcgctgca ataagcgacg gtttggacgg
     4261 ctatgtcgcc cgccgctaca accagaggag ttcgcttgga gtcattctcg acccgattgc
     4321 ggacaaaggc ctgctgctta gcggcatcat tacattgagc atcagtaatt ggagcgaaat
     4381 cgatcccgac tacggaaggt tcccggcatg gttccccgtt ttagttatct cgcgcgatgc
     4441 cgtcattctc gtcggcgccg ccgtcctgta tttgctcaat ggcaaagtgc aggttagacc
     4501 acgatggtcg ggcaaagtcg cgactgtttg ccagatgatc gcgatcggat gggtaatgct
     4561 gcaatttcat tttattcccc tgttttacat tgtgatcggc gctggatttt tcacgctcct
     4621 ctctggcgtg gtttacgtga tggatggcgt gcgccaattg caggccgaag gccacgcgca
     4681 cgcgacgagg cactgagccc gtgtcctggc gatgaacggc gccgcgaata atctgccgaa
     4741 cgtcgcgatc gtcggccggc ctaacgtagg aaaatccgct ctatttaatc gactgattgg
     4801 ccgccgtatc gccatcgtgc atgatcaacc ggggatcacc cgcgaccgga tctcggctgt
     4861 ctgcacgcgc ggaactcagc cgtttgttgt gtgggatacc ggcggcattg ccggtgcagg
     4921 cgaatccgaa ttgaccgcgc aggttcgcct cgctgttcag caagctgttc acgatagcga
     4981 ggttcttctt tttttggtcg atgcaaagac gggcctctca ccgctcgatc aggaactcgc
     5041 gcgcatgctg cgcaagtcgc acaagccagt gattctcgtc attaacaaga tcgacgatgc
     5101 gagacaggag aatctcgccg ccgattttgc atcgcttgga tttcaacaca gtcattccat
     5161 cagcgccgag cacaatcgcg gcatttccga attgcttgaa ccaatcgatc agcttctccc
     5221 ctcgtccgtc gccgcgacag aaatcaaaca tcaaacctca aacatcaaac ctcccgccgc
     5281 catcgccatc gtcggccggc caaatgtcgg caaatcttca ctcatcaatt ccattgtgcg
     5341 cagcgagcgc gccatcgtga gcgagctgcc cggcacaacc cgcgacagcg tcgatatttc
     5401 ttaccagcgg gatggtcagg agtttctctt catcgacacg gcgggaattc gcagacgcgg
     5461 caaacactca agctcagttg aagtcttcag cgtcatgcgc gcggagcgaa gcattcgccg
     5521 cgccgatctc tgcattctca ttgtcgacct gaccatgggt gtaacggctc aagacaaacg
     5581 gattgcagga ctgattcaaa aagcgcgcaa agccgcgatt gttgttttaa acaaatggga
     5641 tttgatcaga ctaaaacgca gggaaaagca gacgatcgcg cgactggtcg atgaaacacg
     5701 cgccagaatt ttctttctcg attatacgcc agtcctggtt gcgtccgcgc tgaccggaca
     5761 aaacatcgaa aagctttttg ccctcatcga aaacgtccgg cgtgccgcgc aacagcgcat
     5821 cggcactggc gttctgaatc gattgctgcg agcggccttc gccgccaacc cgccgccgat
     5881 gatttccgga cggcgactca aattgttata cgctgcacaa gcgagcggaa aacaggagcg
     5941 gccgctgcaa ccccccgagt tcgtcctttt cgtgaacgag cctcggttgt taagcgaaac
     6001 ctatcgtcgc tacctcgaag cgcgcatccg caaagcgcaa ccgtatcccg gattgccgat
     6061 catcctcacg ctgcgtccgc gcagccaaaa ctcgtagcgg ccacgtttga tcgaacattc
     6121 tccttgtcat acgtgtctca tcagaccgta tgaaatcgac gcaggagatt acgcaggagg
     6181 ttcgggagct gggccaatgg attccgccat tgcgccaaca ggtcgggcac gttgtcgttg
     6241 gccagaaata tttggtggat cgccttttgc tcggtctgct cgccaatggt cacatcctgc
     6301 tcgagggcgt tccaggtctg gccaaaacgc ttaccgtaaa aacgatggcc gcgtgcattc
     6361 aaaccggttt tcaaaggctg caatttaccc ccgacctttt gcccgccgat ttgatcggga
     6421 cgctcattta taatccgcgc accggtgaat ttacgacgaa gctcggtcca attttttcca
     6481 atcttatcct ggccgacgag atcaatcgcg cgccggcaaa agtgcagagc gccctgctcg
     6541 aagcgatgca ggagcggcag gtgaccatcg gcgaacaaac ttatcccctc tccgatccgt
     6601 ttctcgttct cgcgacccaa aacccgatcg agcaggaagg aacgtatcaa ttgcccgaag
     6661 cgcagctcga tcgtttcatg ttcaaattga acatcggct
//
LOCUS       DASQUQ010000218         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1060051, whole genome shotgun sequence.
ACCESSION   DASQUQ010000218 DASQUQ010000000
VERSION     DASQUQ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060051"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1268)
                     /locus_tag="VE486_06230"
     CDS             complement(213..1268)
                     /locus_tag="VE486_06230"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HZA38720.1"
                     /translation="MSPLTETYSVGLPVELGKIDRELKKLWEESEGAMTRASLVNLAV
                     YSEEPDSLEKNTQLIAKIAENHACRAIVIGADCKAKENRVEAWISAHCHVSRAGSKQV
                     CSEQISFLLQGPCTTLLPSIVFSHLDSDLPFYLWWQEEFREPMDPQLWAWVDRVIYDS
                     QTWKDFRAQLHLVETAQQEARQRIVLCDLNWTRLDKVRFALAQFFDHPASHHHFSKIK
                     KVRIDFAPGFRSTAILFAGWLAAQLNWRADKAAAQKLQFIGPVDRRIDIELRERQGEP
                     IREIALETSEVEFLVTHAECRDLLEVSRRKPGEKHIPQLVPAGKNDPVSLMSTELMRG
                     GPHDVYLRAVNCIRELL"
     gene            complement(1270..1533)
                     /locus_tag="VE486_06235"
     CDS             complement(1270..1533)
                     /locus_tag="VE486_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZA38721.1"
                     /translation="MTVHFRKSFVKDLEALDQPDRARIEKMILELEATDTLRDIRQLK
                     KLQGRSGFFRIRIGDYRVGLTFLSGVITLVRCLDRKEFYRRFP"
     gene            complement(1530..1736)
                     /locus_tag="VE486_06240"
     CDS             complement(1530..1736)
                     /locus_tag="VE486_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38722.1"
                     /translation="MKSAKSSNDLKEAVKTALIEVLDERQDLLREALTEALEDIGMIR
                     AMDEGLKSGPATREEVFTALRRTG"
     gene            complement(1775..3316)
                     /gene="zwf"
                     /locus_tag="VE486_06245"
     CDS             complement(1775..3316)
                     /gene="zwf"
                     /locus_tag="VE486_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZA38723.1"
                     /translation="MNRAADQPEPNPLREGLSPRAVPQPCTIVIFGATGDLTHRKLIP
                     ALYNLAADGDLPPAVAIVGFARREKSDDQFRSELEEATRKFSRQPVRDEMWKTFAQSM
                     FYHQSEFEDESGYKSLGKRLDKIDKERGTRGNRLFYFAAAPDQFEPILKHLKAAGLND
                     TCKGSWARVIVEKPFGSDLASARELNRIVRSSFTEEQTYRIDHFLGKETAQNILVLRF
                     ANAIFAPLWNTHYIDQVQITAAETLGVESRAGFYERAGALRDMVQNHLLQLLCLIAME
                     PPTDLSADSIRDEKVKVVRSLRRLPRNEVAANVVRGQYTEGAIHGDPVVGYRQEQNVK
                     SDSQTETFVALRLFIDNWRWADVPIYMRVGKRLPKSATEISIHFKKAPAVLFNRGLSD
                     QNVLVIRIQPDEGISLRIQAKVPGASFRIEPVKMDFHYGTSFGKASPEAYERLLLDAM
                     SGDATLFARRDEVEEAWALIDPIEEAWHAKKDAPGLFFYPAGSWGPEEAEELLARDGR
                     AWRRL"
     gene            complement(3378..3830)
                     /locus_tag="VE486_06250"
     CDS             complement(3378..3830)
                     /locus_tag="VE486_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38724.1"
                     /translation="MSEQAPLLRVCSLLNHHGAKYLIVGARACWLHGYVRATMDVDIL
                     IPEELENHLRVIAALSELEDHAAAELTPQDLVENVVVKIADEVEVDVSTRAWKVSYAD
                     AIGTSLTATIEGVKVSYVDLQTLIKSKSTQREQDKVDVQRLLSLNQPD"
     gene            complement(3827..4036)
                     /locus_tag="VE486_06255"
     CDS             complement(3827..4036)
                     /locus_tag="VE486_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38725.1"
                     /translation="MNASKVVGQRKAPRTPAEFHALGARLDRELSVVRPFVRKRGFVF
                     KARSWDELANWESQRLIKEYRQRNQ"
     gene            complement(4047..>4740)
                     /locus_tag="VE486_06260"
     CDS             complement(4047..>4740)
                     /locus_tag="VE486_06260"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZA38726.1"
                     /translation="RLRQIADSVGAILFIDMAHIAGLVAGGQHPSPIPHAQFVTTTTH
                     KSLRGPRGGIVMCEEKFAKQIDSTLFPGVQGGPLMHVIAAKAVCFHEAFQPQFREYQR
                     QVVINAKAIAAGLAKHGYRIVSGGTDNHLMLVDLRPKEINGKEAQEVLDRAGITVNKN
                     AIPFDTYPIFKPGGIRVGTPAVTTRGMKEEEMLEIADLIAEALSNRTDADALERVRRK
                     VLDLTRRFPLPI"
BASE COUNT         1011 a         1332 c         1267 g         1130 t
ORIGIN      
        1 gcgattgaaa aaataattag cgaccgttcg ttagcgcaaa aacttggtca ggccgggcac
       61 gaacgcgctc aaaggttttt ttcgattgag aagaacgtac gggagttgtg cgtgctgctt
      121 ccgtagcggc gcttatgagc gctgaaacgt tgcagccacg ggcctgtggc ccgtcgaacc
      181 ttatgccgaa gagaccgccc acagggcggt ggctacaaca gttcgcgaat gcaattcacc
      241 gcacgcagat aaacatcgtg cggtccgccc cgcatcagct ctgtactcat cagactcacc
      301 gggtcgtttt ttcctgcggg caccaactgc ggaatgtgct tctcgcccgg ttttcggcgt
      361 gaaacttcca ggagatcgcg gcactcggca tgcgtcacga gaaactctac ctcggacgtt
      421 tccagagcaa tttcgcgaat cggttctccc tgtctttcgc gcagttcgat gtcgatcctt
      481 cgatcaaccg gcccgatgaa ttgtaacttc tgtgctgctg ctttatccgc acgccaattt
      541 agttgcgcgg caagccagcc ggcaaagagg atggcggttg atcggaatcc gggcgcgaaa
      601 tcaattcgca ctttcttaat tttagaaaaa tgatgatgcg aggccggatg atcgaagaat
      661 tgggcgagtg cgaagcgaac cttatccaat cgcgtccaat tcagatcgca aagcacgatg
      721 cgctgcctgg cttcctgctg agcggtctca accaaatgca attgcgcgcg aaaatccttc
      781 caagtctggc tgtcgtagat gacacggtcg acccacgccc aaagttgcgg atccatcggc
      841 tcacggaatt cctcctgcca ccagaggtag aacggcagat cggaatccag gtgcgagaaa
      901 acaatactcg gtagcagagt cgtgcacggc ccctggagca ggaacgagat ttgttcggag
      961 caaacttgtt tgctgccggc gcggctgaca tgacaatgcg cgctgatcca ggcttccact
     1021 cgattttctt ttgccttgca atccgctccg ataacaatgg cacggcaagc atggttttcg
     1081 gcaatctttg cgatgagctg cgtgttcttt tccagtgaat cgggctcttc gctgtaaacg
     1141 gcgaggttca ccagagaggc gcgtgtcatt gccccttcgc tttcctccca aagttttttc
     1201 agttcccggt cgatcttgcc tagttcaacc ggaagtccaa ccgagtatgt ttcagttagg
     1261 ggagacatcc tagggaaaac ggcggtaaaa ttccttgcgg tccaaacatc tcaccaaggt
     1321 gatgacccca gagagaaagg tgagtccgac gcgataatcg ccaattcgga tgcgaaaaaa
     1381 accagagcgg ccttgcagtt ttttgagctg acgaatatct cggagcgtat ccgttgcttc
     1441 aagctcaagt atcattttct cgattcgagc tcgatccggc tggtcgagcg cctccaaatc
     1501 tttcacaaaa cttttcctga agtgaaccgt catccggttc ggcgcaaggc tgtgaatact
     1561 tcttcgcgcg tagctggccc ggacttcaat ccttcgtcca tcgcgcggat cattccaatg
     1621 tcctcgagcg cctcggtgag cgcttcgcgc aataagtcct gtcgctcatc aagaacctcg
     1681 atgagcgcgg tcttcactgc ttccttcaga tcgttcgaac tcttcgcgct tttcacagct
     1741 tctacttagt ccatccagat tgaaacagca atattcacaa tctcctccac gcccgaccat
     1801 cccgcgcaag caactcttcg gcttcttccg ggccccacga tccggcggga taaaaaaaca
     1861 aacctggcgc atcctttttt gcgtgccacg cctcctcgat aggatcaatg agcgcccagg
     1921 cttcctcgac ttcgtcgcga cgcgcaaaca atgtggcgtc gccactcatc gcatcgagca
     1981 ggagccgttc atatgcttcc gggctggctt tgccgaagga tgttccgtaa tggaaatcca
     2041 ttttgaccgg ctcgatgcga aaactcgcgc ccgggacctt ggcctggatc cgcaatgaaa
     2101 ttccttcgtc gggttgaatc ctgatgacaa gcacgttttg gtcgctcagg cctctgttaa
     2161 acaggacggc cggcgctttc ttgaaatgga tggaaatctc cgtggccgat ttcggaaggc
     2221 gtttcccgac acgcatgtaa atcggcacat cggcccagcg ccagttgtca atgaaaagac
     2281 gcagggcgac aaacgtttcc gtttgcgaat ccgatttcac gttttgttcc tggcgataac
     2341 ctaccaccgg gtcgccgtgg atcgcgcctt cggtgtattg cccgcgcacg acattggccg
     2401 ctacttcatt acgcggcaag cggcggagcg atcggaccac tttcactttt tcgtcgcgga
     2461 tactgtcagc gctcaggtct gtgggtggct ccatcgcaat gaggcaaaga agttggagca
     2521 ggtgattttg caccatgtcg cgcaaagcgc ccgcgcgttc gtagaagccg gcgcgactct
     2581 cgaccccgag cgtttccgct gccgtgattt gcacctggtc gatgtaatgt gtgttccata
     2641 ggggcgcgaa aatcgcgttc gcgaatcgca ataccagaat gttctgcgcg gtttccttgc
     2701 caaggaaatg atcgatccga taagtctgct cttcggtgaa ggaactgcgg acaatgcgat
     2761 tgagttcccg cgctgaagcc agatcgctgc cgaacggttt ttcgacgatg acgcgagccc
     2821 agcttccctt gcaggtgtcg tttaaacccg cggcctttaa gtgcttcaga atcggctcga
     2881 actggtcggg agccgctgcg aaataaaaaa gccgattgcc gcgcgtaccg cgttccttgt
     2941 cgattttgtc gagacgtttg cccaggcttt tgtaaccgga ctcgtcttcg aactcgcttt
     3001 gatgataaaa catcgattgg gcaaatgttt tccacatttc atcgcgcacg ggctggcgcg
     3061 aaaacttgcg cgtggcctct tccagctcgc tcctgaattg gtcgtcactt ttttctcgtc
     3121 gcgcgaaacc gacgattgcg acggcaggcg gcagatcgcc atcggcagca aggttgtaaa
     3181 gcgctgggat tagcttgcga tgggtgagat cgccagtcgc gccgaaaatc acaatcgtgc
     3241 atggctgcgg cacggcgcgc ggggacaagc cctcgcgtaa tggatttggt tcgggttgat
     3301 cggctgctcg attcatgggc gaagcaaact actcagtttt gacgcgacca ggtaggaaga
     3361 aaattcgggt agcacagcta atccggttgg ttcagcgaga gcaaccgttg aacatcgact
     3421 ttatcctgtt cccgttgggt gcttttactc ttgattaggg tttgcagatc aacatacgaa
     3481 actttcacgc cctcgatcgt tgctgtgagt gatgtgccaa ttgcatcggc ataactgacc
     3541 ttccaggcgc gagtactcac atcgacttcg acttcgtcgg caatcttcac cacgacgttt
     3601 tcgaccagat cctgcggggt caattccgcc gcggcatgat cctcaagctc ggaaagcgcc
     3661 gctatgaccc gcaggtggtt ttccaactcc tcgggaatca ggatgtccac atccatcgtg
     3721 gcccgaacgt aaccgtgcag ccagcaggcg cgtgcgccga cgatcaagta tttcgcgccg
     3781 tgatgattga gcagcgaaca cacgcgcaac aacggagcct gttcgctcat tgattgcgct
     3841 ggcgatattc cttaataagt cgctgcgatt cccaattcgc caattcatcc caactgcgcg
     3901 ctttaaaaac gaaaccgcgt ttgcgcacga atggcctaac gaccgagagc tcgcgatcca
     3961 ggcgtgcgcc gagcgcatga aattccgccg gagttcgcgg ggcctttcgc tggccaacaa
     4021 ctttggacgc attcatttgt cgattcttag ataggcagtg gaaatcgtct ggtcagatcg
     4081 agcactttcc ggcgcactct ctccagtgcg tcggcgtcgg tgcgattgct caatgcttcg
     4141 gcgatcaggt ccgcgatctc cagcatctct tcttctttca tgccgcgggt ggtgaccgcc
     4201 ggagtgccaa cgcggattcc gcccggcttg aaaatcggat acgtatcgaa cggaatcgca
     4261 tttttgttta cggtaattcc ggcgcgatca aggacctcct gcgcttcttt tccgttaatt
     4321 tccttggggc gaaggtcgac cagcatgaga tgattatccg ttccgccgga aacgattcga
     4381 tatccatgct tggccagtcc agccgcaatc gctttggcgt taattacgac ttgccgctgg
     4441 tattcgcgaa attgcggctg aaacgcttcg tgaaaacaaa ctgctttcgc cgcgatcacg
     4501 tgcatgagcg ggccgccctg aactcccggg aagagcgttg agtcgatttg tttcgcgaat
     4561 ttttcttcgc acatcacgat gccgccacga ggaccgcgta aacttttgtg agtggtagtt
     4621 gtcacaaatt gggcgtgcgg aatcgggctc ggatgctgac cgcctgcaac caggcccgcg
     4681 atatgtgcca tgtcgatgaa aagaatcgcg ccaaccgaat cggcaatttg ccgtagccgc
//
LOCUS       DASQUQ010000219         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_415109, whole genome shotgun sequence.
ACCESSION   DASQUQ010000219 DASQUQ010000000
VERSION     DASQUQ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415109"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2386)
                     /locus_tag="VE486_06265"
     CDS             complement(<1..2386)
                     /locus_tag="VE486_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZA38727.1"
                     /translation="MAKEDSSVNPSVSLHEVLETEFAVLHGKLPPDYPSSGDPNVRLT
                     AIWGAVHELSEKRAALCISGGGIRSATFGLGILQGLAHCGLLDKFHYLSTVSGGGYIG
                     SWLSAWINRAGLTNVTTQLARQSEHARPNPEPLEIQNLRSYSNYLSPRLGFFSADSWT
                     IVGTYLRNLLLNWCVIIPLLAAFLALPWIFSSIVMMDPPPRTDALFWSGAALVVIGVA
                     YMGLNLPCGRDLRWNQQRFLLFCLLPLFLASVLMTIFWAWFNYYGRSLIAWPLFGFGQ
                     PQTWVPFLYLGVALHVVSWLTSLLPAHGFRTLEFLAVIVSGAIAGVVLWLGAQKLFPH
                     PVMQMELYICVAVPGFMALFFLAIMVFAGISSRWTDDSDREWWGRATGWLLAVALGWL
                     LISGLVVFGPLSLKWTSSFITTGGLAALLTVLAGRSPMVAASQKERGTASPLGLILSK
                     ASTIAAVIFAAVLLILITEAITSVMKKLIQAYHIKLSYQATLDSKSGLLGRAEEYLNV
                     IFYTPWWLVISFAALLLLAGVAMARLINANKFSLHGIYRERLIRAYLGASNSDRKENP
                     FTGFDENDNIKMRDLWLQEKFHGKLMPVVNIALNLVSGEKLGWQERKAQSFTVSPLHC
                     GSSAMHPGYRSTTGADATVYGGPQGISLGSAITISGAAASPNMGYHSSPFVTFILTLL
                     NVRLGAWLGNPGQAGDHTFHLGYPESSARPIVDEALGLTNDRSPYVYLSDGGHFENLG
                     LYEMVLRRCHFIVISDAGSDPECSFADLGESVRKIRVDFGIPIDFDEMNIYPRSQ"
BASE COUNT          636 a          727 c          681 g          499 t
ORIGIN      
        1 tttggctgcg gggataaatg ttcatctcat cgaagtcgat cggaattcca aaatctaccc
       61 gaatctttcg caccgattca ccgaggtcgg cgaaggaaca ttcgggatcc gagcccgcgt
      121 cgctgattac gataaaatgg cagcgacgca gaaccatttc gtaaagaccg aggttttcga
      181 aatggccgcc atcggagagg tacacgtatg gacttctgtc gttcgttagg cccaatgctt
      241 cgtcaacaat cggccgcgca ctgctctccg gatatccaag gtgaaacgtg tgatcgcctg
      301 cctgtccggg gtttccaagc cacgcgccga gcctcacatt tagcaacgtc aggatgaagg
      361 tgacgaaagg cgatgagtga taacccatgt taggactggc agcggcgccg gaaatcgtga
      421 tcgccgatcc gagcgagatg ccctgcgggc cgccatacac ggtggcatcc gcgccggttg
      481 tagaacgata gcctgggtgc atcgctgacg atccgcagtg caatggactt acagtgaaac
      541 tctgcgcctt gcgctcctgc cagccgagct tttcgccaga caccagattg agcgcgatgt
      601 ttacaacggg catgagtttg ccgtggaatt tttcctgcag ccaaagatcg cgcattttga
      661 tgttgtcgtt ctcgtcgaat cccgtgaatg gattttcttt ccggtccgaa ttcgaagcgc
      721 cgaggtaagc gcgaatcaac cgttcacgat agatgccgtg caatgaaaac ttgttcgcgt
      781 tgatcaggcg cgccatcgcg acgcccgcca gcaaaagtag cgccgcgaac gaaatcacca
      841 gccaccacgg cgtgtagaaa atcacattga gatattcctc agcacgaccg agcagccccg
      901 acttggagtc caacgtcgcc tggtaactca atttaatgtg atacgcctga attaacttct
      961 tcatcaccga tgtgattgcc tcggtgatga gaatcaacag gaccgccgca aagattaccg
     1021 ccgcaatcgt ggaagccttg gacaaaataa ggccgagcgg actcgccgtt cctcgctctt
     1081 tttggctcgc cgcaaccatc gggctgcggc ccgccagaac cgtaagaagc gctgccagac
     1141 ctccggtagt gatgaacgac gaggtccact ttaatgaaag tggtccgaag acgaccagtc
     1201 cgctgatcag cagccatccg agagctactg caagcaacca gccggttgcg cgtccccacc
     1261 attcgcgatc cgagtcgtct gtccagcggc tcgaaattcc ggcgaacacc atgatcgcca
     1321 ggaaaaagag cgccatgaaa ccgggaacag caacgcaaat gtacaactcc atttgcatca
     1381 caggatgcgg aaacaacttt tgcgcgccta accacagaac tacgcccgca atggcgccag
     1441 agacgattac cgcgagaaat tccaatgttc gaaatccatg cgccggaagc aatgacgtca
     1501 gccacgagac gacatgcagc gctactccaa ggtagagaaa cggcacccac gtctgcggtt
     1561 gtccgaagcc gaacaacggc caggcaatca gtgaacgccc gtaataattg aaccaggccc
     1621 agaagatggt catgaggacc gaggcgagga aaagcggcag gagacagaac agcaggaacc
     1681 gctgttgatt ccatcgcaga tcgcggccgc acggcaaatt gagtcccatg taagcgacgc
     1741 caatcacaac cagtgccgct ccgctccaga atagcgcatc ggttcgcggc ggcggatcca
     1801 tcatcacgat cgagctgaaa atccacggca gcgcgagaaa cgcggcgagc aatggaataa
     1861 tcacgcacca gttcaacaac aggttgcgca aatatgtgcc aacaatcgtc caactatccg
     1921 cagagaaaaa cccgagccgc gggctgaggt aattgctgta actacgcaga ttttgaatct
     1981 cgagcggttc cggatttggt ctggcgtgct cgctctgacg cgcgagttgc gtggtaacat
     2041 ttgttaggcc tgcacgatta atccaagcgc tcagccaact gccgatgtac cccccgcccg
     2101 agacggtgga gagatagtgg aacttgtcga gcaaaccgca gtgcgcgagg ccctgcaaaa
     2161 tgccgagacc gaatgtcgcg ctgcgaatcc cgccaccgga gatgcagagt gcggcgcgct
     2221 tttctgaaag ctcgtgcaca gctccccaga ttgctgtgag ccgaacattg gggtcacccg
     2281 aactcggata atcaggcggt aatttaccat gcaaaaccgc gaattcggtc tccagcactt
     2341 catgcaatga aacagaggga ttgaccgatg aatcttcctt tgccatggca cgcaaacgcg
     2401 tccgtctgcg cgttagcgtt tatcaagcct gtggcgttgc gtcaatcctc gctgtgcaac
     2461 caattgcatt gcgttgaaat gcaccgactg ctcagcagct tttattcctc gcgaaacgcg
     2521 gtatatgtct gtgctcatcc tgc
//
LOCUS       DASQUQ010000220         5438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1334820, whole genome shotgun sequence.
ACCESSION   DASQUQ010000220 DASQUQ010000000
VERSION     DASQUQ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334820"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VE486_06270"
     CDS             <1..1044
                     /locus_tag="VE486_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA38728.1"
                     /translation="WKTKGRAEAEIALRLQPDLAEAHFALGQCIYWMDQDYDRALEQF
                     EIASRLSPSNGDLGRFIAAIERRQGKWQESLDEYERVVKVDPQNPNTVLQLIYTSTAM
                     RRWPEAARWAEKMRAMAPASLVAKIQSGYINFWWKGDTRLLKSLLGEIADGVDPDGAV
                     TSGRWDVAMIERDYAAATRALGASSANEISYMNGGATPKAFLYGCIALAQGDRAAAQK
                     YFQDAQAIWENAVKEAPTSATRHANLGLLYAFMGRKADAIREGRRAVELKPESKDAVD
                     GAIMNCYLALIYARVGENDLAIPLIERLLKTAGAVDSADYSITVNDLKQRWEWDPIRN
                     DPRFQKLIASKAL"
     gene            1208..3199
                     /locus_tag="VE486_06275"
     CDS             1208..3199
                     /locus_tag="VE486_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZA38729.1"
                     /translation="MPPESAPDVQLEIGHVLFMDMVGYSKLLLDEQRELQERLNQIVK
                     ETQQVRAAEAAGRLVRVPTGDGMALVFFNSPEAPVRCAIEISQALKESPHIQLRMGIH
                     SGPVNEVQDVNDRTNVAGAGINIAQRVMDCGDAGHILLSKRVAEDLDQARFWRPYLHD
                     LGEYKAKHGVGIFVVNLYTDEVGNPELPTKLKQAQQDVLVKSTSQTRSIAVLPFNDLS
                     PSDDQDYFGDGMAEEILTALAKVDGLRVAARRSSFWFKGKEAELSEIAEKLNVCHVLE
                     GSVRREGKRVRVTAELIDACDGFAIWTETFERELQGIFAVQDEITRSIVDALKLQLDI
                     SPPPPSRSPDAYDAYLQGLFYSDKSTEEALRRSLDFFQRALDKDARFSRAWTGIAKAW
                     LWLADAYVPPLEAYPKVRDAAVRALQLSDDEAEAHVYLAETRRILDWDLDGADAEFNR
                     AVELDPNSTPSNYFIAALYAARGDRDNGLAYLQRTSKIDPASLWVSNFACEIYRYFGL
                     TEEAIAAGERALQLDPAFLFGEPLLAAVYREMGRFDDAIALYKKAESFTGKPGFGLAI
                     TYARMDRRKEAQEILETAVASRGRYTPGDAVAHVHVALHTHDDAIRELERACDERSSS
                     LHMIGIAPEFAPLRSDRRFISLLKKIGLEPEKVFASSPL"
     gene            complement(3275..4144)
                     /locus_tag="VE486_06280"
     CDS             complement(3275..4144)
                     /locus_tag="VE486_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZA38730.1"
                     /translation="MNLQTETSHFEPPKAETTWSRVKKALGPIAVVGFLIVKFFSKLK
                     LVFLPLLKFLPVLLKSGGTMLLMIWVYMQLWGWQFAVGFVLLLLVHETGHLLVAKKFG
                     LKVGAPVFIPFMGAFIALKEAPRNAWIEACVGIGGPMLGTLGALLCNSLGEVFNAPLF
                     IALAWFGYFLNLFNLTPVGMLDGGRIVTALSRWLWLPGFALLVWFGWKYPNFIIWLIV
                     LLSLPRIYSLFRKRTEEEQRYFEVTPSQRWTMSILYFGLIAVLIFGMHVAQQDLNKHG
                     VRAHGHGQDTLVQ"
     gene            complement(4168..>5438)
                     /locus_tag="VE486_06285"
     CDS             complement(4168..>5438)
                     /locus_tag="VE486_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38731.1"
                     /translation="GLFAEKDWLLSPDAFPIEDKFLAELEQLGHRLFVFQRACNQLYQ
                     LSVKGKQPAWVTRYLDAGKPADLIEFSRRKEIRDDLPRVIRPDLILTEQGYIIAEIDS
                     VPGGIGLTGWLNQTYSAFDTEIIGGADGMRDGFKSVLPDGGDIVISQEAATYRPEMEW
                     IAARLNQTSNVKDQTSLWRVVAAENYEPQVGRAVYRFFELFDLPNIPKINKTLRANAE
                     GRITITPPIKPHLEEKMWFALFWMQPLKEFWRRELGEKYFLKLQEVIPYSWLLDPAPL
                     PQHAVIPRLEIHDWREAAKFSQKDRELLLKVSGFSPLGWGSRGIALGADLPHAEWERR
                     IDHALATFESSPTILQRFHKGRLFEHRYWDAETGELKTMKGRVRLCPYFFVERDRVKL
                     RGALATIVPADKKFLHGMRDAILVPSRVGA"
BASE COUNT         1274 a         1491 c         1540 g         1133 t
ORIGIN      
        1 tggaaaacaa aggggcgcgc cgaagcggaa attgccctgc ggctccaacc agatctggcg
       61 gaagcacact ttgccctggg gcaatgcatt tactggatgg atcaggacta tgaccgcgcg
      121 ctcgagcaat tcgagatcgc atctcgctta tcgccgagca acggagattt aggtcgcttc
      181 attgcagcaa ttgaacgccg gcagggcaaa tggcaggaat cactcgatga atacgaaaga
      241 gttgtgaagg tcgatccgca aaatccgaat accgtcctcc aattgatcta tacgagcacg
      301 gcgatgcgtc gctggccgga agcggcccga tgggcggaga aaatgcgggc gatggcgccg
      361 gcctctctcg tcgcgaagat tcaaagtggc tatatcaatt tttggtggaa gggcgacacc
      421 cgtttgctga aatctttgct tggcgaaatt gcggatggtg ttgatcctga cggcgccgtt
      481 acgtctggtc gttgggacgt ggcgatgatc gaacgggatt acgcggccgc gacgcgcgct
      541 ctcggtgctt cgtcagcgaa cgaaatatcg tacatgaatg ggggagcgac accgaaagcg
      601 tttctttatg gatgcatcgc gctggcgcaa ggcgatcgcg ctgcggcgca aaaatatttt
      661 caagatgcgc aagccatctg ggaaaatgct gtcaaagagg cgccaacgag cgccacacgg
      721 cacgcgaacc ttggcctgct ctatgccttc atgggacgca aagctgatgc gatcagggaa
      781 ggccgccgcg ccgtggaact taagccggaa tcaaaagatg ccgtcgatgg cgcgatcatg
      841 aactgttacc ttgcgctcat ctacgcgcgc gtcggagaaa atgatctcgc gattccgctc
      901 atcgagcgct tactcaagac agcgggcgca gtcgacagcg ccgattacag catcacggtc
      961 aacgatctga aacagcgttg ggaatgggac ccgattcgga acgacccgcg ctttcaaaag
     1021 ctaatcgcga gcaaggcgct gtgaacgcgt ggtccgctcc gctgaattac gattctcact
     1081 caattcgctc ccatgctagc ggggcgttgt tgctgtcaaa cttcaatctg ccgattgcca
     1141 atcgttaacg aaatacgaag cttgatctca ttgaaattcg aaagagcctc ctttcgattc
     1201 aatccgtatg cctcctgaat ctgcaccaga cgttcagtta gagatcgggc atgtcctgtt
     1261 catggacatg gttggttact cgaagctgct cctggacgaa cagcgcgagc tccaggaacg
     1321 actcaaccaa atcgtcaaag aaacgcaaca ggtgcgggcc gcggaggcag ccggcaggtt
     1381 ggtccgtgtt cccaccggtg acggaatggc gcttgtcttt tttaatagtc cggaggcgcc
     1441 ggtccgctgt gcgatcgaga tcagccaggc gctcaaagaa tcaccgcaca ttcagctgcg
     1501 gatgggcatc cacagcgggc cggtcaatga agttcaagat gtgaacgacc gaacgaacgt
     1561 ggccggcgct ggaattaaca tcgcccagcg ggtgatggat tgcggcgacg ccggccacat
     1621 ccttctctcc aagcgcgtag ccgaagattt ggatcaggcc cggttctggc gtccatacct
     1681 tcatgatttg ggcgagtaca aggcaaagca cggcgtcggg atatttgtgg tcaatcttta
     1741 taccgatgaa gtcgggaatc cggaattgcc gacaaaactc aagcaggcgc agcaggatgt
     1801 cctggtaaaa tcgacatcgc agacgcgatc gatcgccgtg ttgccgttta acgatctgag
     1861 cccgtcggac gatcaggatt attttggcga cggcatggcc gaagagatcc tcactgcgct
     1921 ggcgaaagtg gacggcctgc gcgtagccgc gcgacggtct tcgttttggt tcaaaggcaa
     1981 ggaggcggag ctgagcgaga tcgcggaaaa gttgaatgtg tgccacgtgc tcgaaggcag
     2041 cgtccggcgc gagggcaaac gcgtccgggt gacggcggaa ttgatcgatg cctgcgatgg
     2101 attcgcgatt tggacagaaa cattcgaacg cgagttgcag ggaatttttg cggtccagga
     2161 cgagatcacg cgctcgatcg tcgacgcgct caagttgcaa ctcgatattt cccctccgcc
     2221 gccgtctcgc agccccgacg catacgacgc ttatttgcag ggcctttttt attcggacaa
     2281 aagcaccgag gaggcgttgc ggcgaagtct ggattttttt cagcgcgcac tggacaaaga
     2341 tgcgcgattc agtcgcgcgt ggacgggaat cgcgaaggca tggctgtggc tggcggacgc
     2401 ttatgtgccg ccgctcgaag cctatccgaa agtccgtgac gcggccgttc gcgctttgca
     2461 gctcagtgac gatgaggccg aagcgcacgt ctatctcgct gagacaaggc gcatcctcga
     2521 ttgggatctg gacggcgcgg acgcggaatt caatcgcgcg gtcgagctcg atccgaattc
     2581 aacgccgtcc aattatttca tcgctgcgct ttacgccgcc cgcggtgacc gcgacaacgg
     2641 actggcttac ttgcaacgca catcgaagat cgatcctgct tcgctttggg tcagcaattt
     2701 cgcttgcgag atctaccgtt acttcggttt aacggaggag gcgatcgctg cgggggaacg
     2761 cgcgttgcaa ctggatccgg cctttctgtt tggcgaacca ttgctggcgg cagtctatcg
     2821 tgaaatgggg cgcttcgatg acgccatcgc gctgtataag aaagcggaga gtttcactgg
     2881 caaaccgggg ttcggtctgg cgattaccta cgcgcgtatg gaccggcgca aggaagcgca
     2941 ggaaattctc gaaacagccg tggcaagccg gggacgctac acgcccggcg atgccgttgc
     3001 acatgtccac gtggcgttgc atacgcacga cgacgcgatt cgagaactcg aacgcgcctg
     3061 tgacgagcgc tcgtcatcgc tccacatgat cgggattgct cccgagttcg cgccgctgcg
     3121 ttcggatcgg cgttttatat cgctcttgaa gaagatcggg ctcgagccgg aaaaggtctt
     3181 cgcgagctct ccgttgtaac tttttaacta ttttaacctt gtaacaaagg tgaaacagga
     3241 gggacaggaa gcgcatgatc acgagcggat tcgcctactg caccagcgtg tcttgtccgt
     3301 ggccgtgagc gcgcacaccg tgcttgttca aatcctgctg ggcgacgtgc atcccgaaga
     3361 tcagcacggc aatcaaacca aaatataata tcgacatcgt ccaacgctgc gatggcgtga
     3421 cttcgaaata gcgttgctct tcttcagttc gcttgcggaa caacgaataa atccggggca
     3481 gcgacagaag cacgatcagc cagataataa agttcggata cttccacccg aaccaaacca
     3541 gcaacgcgaa tcccgggagc caaagccagc gcgacaatgc ggtcacgatt cgcccgccat
     3601 cgagcatccc aaccggcgtc aggttaaaga ggttcagaaa atagccgaac caggcgagcg
     3661 cgataaaaag tggcgcgttg aagacttcgc cgagcgaatt gcacaacaag gcgccgaggg
     3721 tgcccagcat cgggccgccg atgcccacac atgcctctat ccatgcattc cggggcgctt
     3781 ccttcagcgc aatgaaagcg cccatgaacg gaatgaaaac cggcgcgccg actttgagcc
     3841 caaatttttt tgcgaccagc aaatgaccgg tttcatgaac caagagaaga aggacgaacc
     3901 ccactgcgaa ctgccagccc cataattgca tgtaaaccca gatcatgagc aacatggtgc
     3961 ccccggattt cagcagaacg ggcaggaact tcagaagcgg caaaaaaaca agctttagct
     4021 tcgagaaaaa ctttacgatc aagaagccaa ccacggcgat tgggcccagc gcttttttga
     4081 cccgactcca cgtcgtctca gccttcggtg gctcgaagtg gcttgtctcg gtttgaagat
     4141 tcattgcttg taattacgcg cttgcagcta agctccgacc cgcgacggca caagaatagc
     4201 gtcgcgcatt ccgtgcaaaa atttcttatc tgccggcaca atagtggcga gagcaccgcg
     4261 cagtttaacg cgatcacgtt ccacaaagaa atacggacaa agacgcacgc gtcctttcat
     4321 tgtttttaat tcgccagttt cagcgtccca atagcgatgt tcaaagagac ggcccttatg
     4381 aaagcgctgc aaaatcgtgg gcgacgattc gaacgtcgcg agcgcatggt cgatcctgcg
     4441 ctcccattca gcgtgcggca gatcggcgcc gagcgcaata ccgcggctgc cccagccgag
     4501 cggtgaaaat ccgctgacct tcaaaagaag ctcccggtct ttctggctga acttcgcggc
     4561 ttctcgccaa tcgtgaatct ccaggcgtgg gatgaccgcg tgttgcggca atggcgccgg
     4621 atcaaggagc caggagtagg ggataacctc ctgtaacttg agaaaatact tttcacccag
     4681 ttcccgccgc caaaattctt ttagcggctg catccaaaaa agtgcgaacc acatcttctc
     4741 ttcgagatgc ggtttaatgg gcggggtaat agtaatgcgt ccctcggcgt ttgcgcgcag
     4801 tgtcttgttg attttcggaa tattcggcaa atcgaacagc tcgaagaatc gatagacagc
     4861 gcgtccgact tgtggctcgt aattttcggc cgcgaccacg cgccacagag atgtttgatc
     4921 tttgacgttt gatgtttgat ttaatcgcgc ggcgatccac tccatctccg gccggtacgt
     4981 ggctgcctcc tgagagatga caatgtcgcc gccatcgggc agaacactct tgaatccatc
     5041 ccgcattcca tctgcgccgc cgataatctc agtgtcgaac gccgaatacg tctggttaag
     5101 ccaacccgtt aatccaattc caccaggcac ggaatcgatt tcagcaatga tgtaaccttg
     5161 ctcggtcaga atcaggtcgg gccgaatcac ccgcggcaaa tcgtcgcgaa tttctttgcg
     5221 tcgcgaaaac tcgatcaagt ccgccggttt gcctgcatcg aggtaacgcg tcacccacgc
     5281 aggctgtttt cctttgacgc tcaattgata aagctgattg caagcgcgtt gaaaaacgaa
     5341 caagcgatgg ccaagctgct cgagctcggc gagaaatttg tcttcgatcg gaaaggcgtc
     5401 gggtgaaagc agccaatcct tctccgcaaa gagaccct
//
LOCUS       DASQUQ010000221         3690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_523694, whole genome shotgun sequence.
ACCESSION   DASQUQ010000221 DASQUQ010000000
VERSION     DASQUQ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523694"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VE486_06290"
     CDS             <1..647
                     /locus_tag="VE486_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38732.1"
                     /translation="ALRRVTTFMAEKLRPTEQMLELSEDDTLSAEHLDSQVQKAQEQL
                     LQLKRQQEQIEKQKRELEELSRRQEELERGRAEMTDKLTRALVVLERDAYNAQTRLEQ
                     IRATRESFGQHLELVEAIDPKSWNPGDLHKELSRALSTVDEARTEYSEQRSRLQATVE
                     NAGDTPLPDVGLEAYENTGGRSFSQWFQIGVALTLPLIIFGIIALAIFFWMASR"
     gene            647..1018
                     /locus_tag="VE486_06295"
     CDS             647..1018
                     /locus_tag="VE486_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38733.1"
                     /translation="MAMFRRSPKPPSALRRRQEEIEQQEAELRQKVEKLERMVVNAPL
                     SADDPAMGPRRKRGVRRDAGDKQLHVSVALEDRHYFDKSGSRRRPRALRKARREGRIV
                     FLLLVAALAVAVIWLMSHLHF"
     gene            1049..1339
                     /gene="gatC"
                     /locus_tag="VE486_06300"
     CDS             1049..1339
                     /gene="gatC"
                     /locus_tag="VE486_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZA38734.1"
                     /translation="MAKTSDLDVAYVAGLARLNLTDEETALFQKQLGDVLNYAEKLRE
                     VDVSDVESAAHALPIFNVFREDAPRDWFTAQQALSNAPRQANGLFIVTKVVE"
     gene            1336..2814
                     /gene="gatA"
                     /locus_tag="VE486_06305"
     CDS             1336..2814
                     /gene="gatA"
                     /locus_tag="VE486_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZA38735.1"
                     /translation="MTGKDLPALSIADLQSLLRNREISPREVLEALRARIEAVDGEIA
                     AYLSLDFEAAAREAEKANVDLPLGGVPIAIKDIINVAGEPCTCASKILRGYRAPYDAT
                     VVRKLRAAGAIPFGKTNMDEFAMGSSTENSAMKLTRNPWDLSRVPGGSSGGSAAAVAA
                     NEAFGALGTETGGSIRQPAALSGVVGLKPTYGRVSRFGVVAFASSLDQVGPLTKTVRD
                     SALIMNAIAGHDEQETTSLNEPVPDYTARLGEDLRGFRLGLAKEYMVEGTDPQVKAAI
                     NAAVKHLNSLGAEIVDVSLPHTDYATAVYYILATAEASANLARFDGVRYGYRAENPKD
                     LLDLYGRTRGEGFGLEVKRRIILGTYVLSSGYYDAYYLRAQKVRELIRRDFARAFEKV
                     DALISPTSPVPAFKFGERTADPLQMYLADIFTSSANLAGICGISVPCGFTEADGQRLP
                     IGLQLLGKALDEARILQIAHAYEQSTDWHKARPPIAASRQNA"
     gene            2964..>3690
                     /locus_tag="VE486_06310"
     CDS             2964..>3690
                     /locus_tag="VE486_06310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZA38736.1"
                     /translation="MDYELWYDTGKHVLAGDEIFFFRSGKYDFMYPPTCALFLAGASL
                     LGQAGLILLLVVINSAAWFHGIRLAAILAAGERHVKNALLYLVPSLLLIVYIWSSYHL
                     GQPHLVLLALMLGAFAALRAKREVCAGALIAIAASIKAFPAIAIIYLVYRRYWKAAAS
                     LIVTLAFLLLILPAPFRGFERAWRDLEKWSVGMLKYDATGVAQRPLRSHSWKNQSLVG
                     VANRMLRHIDSDPVPGSHTPIYVN"
BASE COUNT          798 a          990 c         1097 g          805 t
ORIGIN      
        1 tggcgctgcg gcgagtcacg actttcatgg cagaaaaact tcgaccgact gagcagatgc
       61 tggaactgag tgaggatgac acgctctccg cggagcatct ggactcgcaa gtgcaaaagg
      121 cgcaggaaca attacttcaa ctcaagcgcc agcaggaaca gatagaaaaa caaaagcgcg
      181 agctcgaaga actcagccgt cgccaggaag aattggagcg cggtcgcgcg gaaatgactg
      241 acaagctgac gcgcgcgctc gttgtgctcg agcgcgacgc gtataacgcg caaacgcggc
      301 tcgagcagat ccgggccact agagaaagct tcggtcagca tctggaattg gttgaagcga
      361 tcgatccaaa aagctggaat cccggagatt tacacaagga attgagtcgt gccttgagca
      421 cagtggatga ggcacgcacc gagtacagcg agcagcgcag ccggctgcaa gcaaccgtcg
      481 agaacgctgg cgatacgccg ttgcctgacg tcgggttgga ggcgtacgag aacaccggcg
      541 gacgttcatt ttctcaatgg ttccaaattg gcgtcgccct gacgttgccg ctgattattt
      601 tcggcattat cgctcttgct attttcttct ggatggccag ccggtgatgg cgatgtttcg
      661 tcgctcgcct aaaccacctt cggcgctccg tcgcagacag gaagagatag aacaacagga
      721 agcggaactg cgtcagaagg tcgaaaaact cgagcgcatg gttgtaaacg cgccgctgag
      781 cgcggatgac ccagccatgg ggccaagaag gaaacggggc gtaagacgtg atgcaggcga
      841 taagcaactc cacgtctcag ttgccctgga agacagacac tacttcgata aaagcggaag
      901 cagacggcgg ccgcgtgcgc tgcgcaaagc gcgacgggag gggcggatcg tttttctgct
      961 ccttgtagcg gcgctcgcgg tagcggtcat ctggctgatg agccatctgc acttttgacc
     1021 ccgaaagctt tcggggttga ttggcatcat ggcgaagact tccgacctcg atgtcgcgta
     1081 cgtcgccggg ctggcgcggc taaatctgac cgacgaggaa acggcgcttt tccagaagca
     1141 gctcggcgat gtattgaatt acgctgagaa actgcgcgaa gtggatgtga gtgatgtcga
     1201 atcggcggct catgcgctcc caatttttaa cgtgttccgg gaagacgcgc cgcgcgactg
     1261 gtttacggcg cagcaagcac tgagcaacgc gccccgccag gcaaacggcc ttttcatagt
     1321 cacgaaagtg gtggaatgac ggggaaagat ctgccggcgc tgagcatcgc cgatttgcaa
     1381 tccctgttgc gtaaccggga gatctcaccg cgcgaagtac tcgaggcgct cagagcacgc
     1441 atcgaagctg tggatggaga aattgccgcc tatctctcgc tcgactttga agctgcagcc
     1501 agggaagcgg agaaagcgaa tgtcgatctt ccgttgggcg gcgtgccgat cgcgatcaaa
     1561 gacatcatta acgtcgcagg agagccctgc acctgtgcgt caaaaatctt gcgcggctat
     1621 cgcgcgcctt acgatgcgac cgtcgttcga aaattgcgcg ccgcaggcgc aattccattt
     1681 ggcaaaacca atatggacga attcgccatg ggttcgtcga ccgaaaattc cgcgatgaaa
     1741 ttgacgcgca atccgtggga cttgtcgcgt gtgccgggag gttccagtgg cggttcggcc
     1801 gcagcggttg cggccaatga ggctttcggg gctctcggga cggagacggg cggatcgatt
     1861 cggcaaccgg ccgcgctttc aggggttgtt ggtctgaaac cgacctatgg ccgtgtctcg
     1921 cgcttcgggg tggtcgcgtt cgcttcgtcg ctcgatcaag ttggtccgtt aacgaaaacg
     1981 gtgcgggatt cagcgctgat catgaacgca atcgccggcc atgacgaaca ggaaacaacc
     2041 tctttgaacg aaccagtgcc tgattatacc gcccgcttgg gagaagattt gcgcggtttc
     2101 cggttgggcc tggcgaagga atacatggtc gaagggaccg accctcaggt caaagccgcg
     2161 atcaatgcgg cagtcaaaca tctgaattcg ctgggcgcgg agatcgttga tgtctccctg
     2221 ccgcataccg attatgcgac cgcggtttat tacatcttgg ctacggcgga ggcttccgcg
     2281 aacctggcgc gctttgatgg tgtgcgttac ggatatcgag cggaaaatcc aaaagatttg
     2341 ctcgatcttt atggccggac acggggcgag ggttttggct tggaagtaaa acgccgcatc
     2401 attctcggca cttatgtttt gagctcgggt tattacgatg cctattattt gcgtgcccag
     2461 aaagtgcgcg agttaatccg ccgggatttt gcaagggcct tcgagaaagt cgatgcgcta
     2521 atttcgccga cgtcgcccgt gccagcgttt aaatttggcg aacgcaccgc ggatccattg
     2581 caaatgtatc tcgccgacat tttcacgagc tccgcaaatc tcgccggaat ttgtggaatc
     2641 agcgtgccgt gcggttttac cgaggccgat gggcagcgcc tgccgatcgg tctgcagttg
     2701 ttgggcaaag cactcgatga agcgcgcatt ttgcagatcg cgcacgccta cgagcagagc
     2761 acggactggc acaaagcgcg tccgccgatc gctgcgagcc ggcagaacgc gtgaggaatt
     2821 caccttcaag gacgaattgg gcgacgctca atcgccgatt aaatagcccg accggttttc
     2881 gcgtcctgct gtcgatcttt attgtgacag cgatcgtctt ttcgaccgta ccgctcctcc
     2941 actatttccg cggccgcagc atcatggatt acgaattgtg gtacgacacc ggcaaacatg
     3001 tgctcgcggg tgatgaaatc tttttctttc gctctgggaa gtacgatttc atgtacccgc
     3061 cgacttgtgc gctgtttttg gcaggcgcga gtttgctggg acaggccgga ttaattcttt
     3121 tgctcgtcgt catcaactca gcagcctggt tccatggcat tcggctggcg gcaatacttg
     3181 cggctggcga acgccatgta aaaaatgcat tgctctatct ggttccgagt ctgctgctga
     3241 tcgtttacat ctggtccagt taccatctcg gccaaccgca tctggtgttg ctcgcgctaa
     3301 tgctaggcgc tttcgcggcg ctgcgcgcaa aacgggaagt ttgcgccggt gcactgattg
     3361 cgattgccgc ctcgatcaaa gcctttccag cgatcgcaat tatttatctg gtttaccggc
     3421 gttactggaa agcggccgcc agccttatcg tgactctcgc gtttttactt cttattttac
     3481 cggcgccttt ccggggattt gaacgagcat ggcgcgacct cgagaagtgg agcgtcggaa
     3541 tgttaaaata cgacgcgacc ggcgtggccc agcggccgct gcgaagccat tcatggaaaa
     3601 atcaatcgct tgtcggtgta gccaatcgca tgcttcggca catcgattct gatcccgttc
     3661 ctggatcgca tacccccatc tacgtcaacg
//
LOCUS       DASQUQ010000222         3826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_231029, whole genome shotgun sequence.
ACCESSION   DASQUQ010000222 DASQUQ010000000
VERSION     DASQUQ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231029"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /gene="pstA"
                     /locus_tag="VE486_06315"
     CDS             <1..804
                     /gene="pstA"
                     /locus_tag="VE486_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZA38737.1"
                     /translation="QGKKMELSDREFRKWQETHKEPVDATSIAYSAGGIWPCIVGTAL
                     LVIGSMILALIIGISAAIYLSEYSRETRLIRFIRVAILNLAGVPSIVFGLFGFGLFVI
                     FFGWNVSLIAGWFTLGLMILPAIITASEESLRAVPQGLREGSLALGATMWQTIRKNVL
                     PYALPGILTSSILGIARVAGETAPIMFTAAYAMRDEMPWQVTKWTDFFFQGVMALPYH
                     IYVVSSKIPQNEYTERVQYGTAFVFLVVVMLIALTSIVLRERQRRRLSW"
     gene            798..1643
                     /gene="pstB"
                     /locus_tag="VE486_06320"
     CDS             798..1643
                     /gene="pstB"
                     /locus_tag="VE486_06320"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZA38738.1"
                     /translation="MVKVTDAPETTRQQTPPQPFEPVENEAEKLQVMIDVRDVDFTYG
                     DNKVLHNISLQMPPRAVTAFIGPSGCGKTTLLRCINRMNDLIDGARITEGAIHVDGID
                     INTPEVDVVDLRRRVGMVFQKSNPFPKSIYENISYGLRIAGINKRSLIDEAVEKSLRG
                     GALWDEVKDRLNANAFGLSGGQQQRLCIARALAVEPEVILMDEPCSALDPIATAKIEE
                     LIHELKTQYTIVTVTHNMQQAGRISDYTAFFYLGRLIEFGSTAKIFTNPSEKQTEDYI
                     TGRFG"
     gene            complement(1732..2169)
                     /locus_tag="VE486_06325"
     CDS             complement(1732..2169)
                     /locus_tag="VE486_06325"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HZA38739.1"
                     /translation="MKKKVLFICVHNSARSQMAEAWLNHICPDDFEASSAGLEPGTVN
                     PLAVEAMREVKVDISGKKTQAVFDVFKSGEFFPYVITVCDESEAAGCPIFPGVTQRIH
                     WSFPDPSAFTGSYEERLERTREIRDQIRDRIQMWCDEVCGVEA"
     gene            complement(2198..2881)
                     /locus_tag="VE486_06330"
     CDS             complement(2198..2881)
                     /locus_tag="VE486_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZA38740.1"
                     /translation="MKKYGAEFIGTFALVFAGTGAIIINDVSGGAVTHVGVALTFGLV
                     VLAMIYAVGDVSGAHLNPAVTLGFFAARRLRRKMVLPYIISQFLGAIVASGVLRALFP
                     RHPTRGITVPAGSASQSLILEIILTALLMFVILNVSTGPKERGTTAGIAVGAVIALEA
                     LFAGPISGASMNPARSFAPALVTQHFADLWIYLVAPATGALLAVIGCRCVREEGCCSA
                     LSAETSSVI"
     gene            complement(2927..3523)
                     /locus_tag="VE486_06335"
     CDS             complement(2927..3523)
                     /locus_tag="VE486_06335"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZA38741.1"
                     /translation="MKRTFPRSVAAWAFLFVLLGTVASRGSSLSTEVIVSVPDQVLAL
                     VDRGRLIARYPISTSKFGIGDSAGSYRTPLGTLFVSAKFGDNLPPGAVIKNRVPTGEV
                     VTADAPGRDPIVARVIWLRGLEAQNRGARDRCIYIHGTPEERRIGKPASFGCIRMRSR
                     DVIALYDSVHIGMHVVITRKQISDFIQADEPSLLARSD"
     gene            complement(3520..>3826)
                     /locus_tag="VE486_06340"
     CDS             complement(3520..>3826)
                     /locus_tag="VE486_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38742.1"
                     /translation="TPVTMLAGELAVLRRDLRKTIRAYASRLEVALAESVAAVSSKRA
                     EALSREQLHDIRDLTIMVRKRKLKPEKGRRKDLRKIDSLIDDLHSITHPAGSQNSLK"
BASE COUNT          879 a         1058 c         1053 g          836 t
ORIGIN      
        1 cagggcaaaa aaatggagct gagcgatcgc gaattccgga aatggcagga aacgcacaag
       61 gaacccgttg atgccaccag catcgcttat tctgccggcg gaatctggcc ttgtatcgtt
      121 ggcacggcgc tgctcgtcat cgggtccatg atcttggcct taatcatcgg gatcagtgcg
      181 gccatttatc tgagcgaata tagtcgggag acacgcctta ttcgcttcat tcgcgtggca
      241 attttaaatt tggctggagt tccctcaatc gtattcggcc ttttcggttt tgggctgttc
      301 gtaattttct ttggctggaa tgtttcgctt attgctggct ggttcacact gggtctgatg
      361 atcttgccag cgatcattac cgccagcgaa gaatcgttgc gcgctgtgcc acaaggactg
      421 cgcgagggtt cgctcgcgct tggcgcgaca atgtggcaaa ctatccgcaa gaatgttctg
      481 ccctacgcgc tgccgggaat tttaacgtcg tcgatcctcg gcatcgcccg cgtcgcaggt
      541 gagaccgcgc cgatcatgtt tacggcagct tacgcaatgc gcgatgaaat gccctggcag
      601 gttaccaaat ggactgattt ctttttccag ggtgtcatgg cgctcccgta tcacatttat
      661 gttgtcagct cgaaaattcc gcaaaacgaa tacaccgaac gcgtccaata cgggacggcg
      721 tttgtttttc tggtagtcgt gatgttgatt gcattaacct cgattgtgct gcgggagaga
      781 caaaggagac gcttatcatg gtaaaggtaa cggacgcgcc agaaacaacc cgccaacaga
      841 cgccaccgca acctttcgaa ccagtggaaa acgaggcgga gaaactccag gtgatgatcg
      901 acgtccgcga tgtcgatttc acctacggcg ataacaaggt cctccacaac atttccctcc
      961 aaatgccgcc gcgcgcggtg acggccttca tcggcccgtc gggctgcggc aagacgacac
     1021 tccttcgctg catcaatcgc atgaacgatc tcattgacgg ggcacgcatc accgagggcg
     1081 cgattcacgt tgacggcatc gatatcaaca cacccgaggt agacgttgtc gatcttcgcc
     1141 ggcgtgtcgg gatggtcttt caaaaatcga atccgtttcc caaatcgatt tacgaaaaca
     1201 tttcctacgg cctgcgcatc gcggggatta acaagcgttc gcttatcgat gaagcggtcg
     1261 aaaaaagttt gcgcggcggg gcgctatggg acgaagtgaa ggaccgcttg aatgcgaacg
     1321 cttttggcct ctccggcggc cagcagcaga ggctttgcat agcgcgtgcg ctcgccgttg
     1381 aaccggaagt gattctgatg gatgagcctt gttcggcgct tgacccgatc gctacagcaa
     1441 agatcgaaga gctgattcac gaactcaaaa cgcaatacac gatcgtgacg gtgacccaca
     1501 acatgcagca ggccggtcgc atctccgatt acacggcctt cttttatctt gggaggctga
     1561 tcgaatttgg atcgaccgcg aaaattttca caaatccgag cgagaaacag accgaagatt
     1621 atatcaccgg ccgattcggt taagagcggt tcacgactgg tcttttcggc aggcaaaatc
     1681 cgctgtgcga cgataaacac gcttaagtca gctcagtcgg tcggaagcgc gtcacgcctc
     1741 gaccccgcac acttcatcgc accacatctg gatgcggtcg cgaatctgat cgcggatctc
     1801 gcgcgttcgt tcgaggcgtt cttcgtaact gccggtaaac gcagaaggat cgggaaagct
     1861 ccagtgtatt cgctgcgtca cgcccggaaa aatcgggcag cctgctgctt ccgattcatc
     1921 gcacacggta atgacgtatg ggaaaaattc tccgctctta aagacgtcaa agacggcctg
     1981 tgttttcttg ccggaaatat ccactttgac ctcgcgcatt gcttcaaccg cgagtggatt
     2041 gaccgtcccc ggctcaagtc ctgcgctgga cgcctcgaaa tcatcgggac aaatatgatt
     2101 cagccacgct tccgccattt ggctgcgggc actgttgtga acgcaaataa acaagacttt
     2161 ctttttcata aattcgttcc tccggtcggt cagactttta tattacagaa cttgtctcgg
     2221 cagacagagc cgagcaacag ccttcttccc ttacgcatcg acatccaatc acggcaagta
     2281 aagcgccggt tgccggggca accaggtaaa tccagagatc ggcaaaatgt tgcgtgacca
     2341 gtgcgggtgc gaatgaacgc gcaggattca tcgaggcacc ggagatcggt ccggcaaaga
     2401 gcgcttccag tgcaatgaca gcgccgaccg caattcccgc ggtcgtaccg cgttcctttg
     2461 gcccggtgga gacgttgagg atgacgaaca tcaacagcgc ggtgaggatg atttccagga
     2521 tgagtgattg actggctgaa ccggcgggca cggttatacc acgtgtcgga tgccgaggaa
     2581 acaatgcgcg caacacgccg ctggccacga tagcaccgag gaattgactg ataatatacg
     2641 ggaggaccat tttcctgcgc agccggcgcg ccgcgaaaaa accgagggtc accgcgggat
     2701 tgagatgcgc gccggagaca tcgcctaccg cgtagatcat cgcgagcacg acaaggccga
     2761 aagttagcgc cactcctacg tgcgtaactg cgccgccgct tacatcgtta attatgattg
     2821 cgcccgtgcc cgcaaagacc agcgcgaacg tgccgataaa ttctgcgcca tacttcttca
     2881 tcgaagtgag atcaaaaaaa tggcctggca atggttagcg ctcagattaa tccgaccttg
     2941 cgagcaaact cggttcgtcc gcctgaatga aatcgctaat ttgttttcgg gtgatgacca
     3001 cgtgcatgcc gatgtgcact gagtcataaa gcgcgatcac gtcgcgtgag cgcatacgga
     3061 tgcagccaaa actggccggt ttgccgatcc ggcgctcttc aggtgtgccg tgaatgtaga
     3121 tacaacgatc gcgcgcgccg cggttttgcg cttccagtcc acgcagccag atgacgcgag
     3181 caacgatcgg gtcccggccc ggcgcgtcag cggtcacgac ttcgcccgtt ggaacccgat
     3241 tcttgatgac cgcgcccggc ggcaagttgt cgccgaattt ggccgaaaca aacaaagtgc
     3301 ccagaggcgt acggtaactg ccggcggaat cgccgattcc aaatttggaa gtcgaaatcg
     3361 gataacgcgc gatgagacgt ccccggtcga ccagcgccag aacttgatcg ggcacgctga
     3421 caattacttc ggtgctgagg ctcgacccac gggatgcaac tgtgcccaaa agaacaaaca
     3481 gaaacgccca tgctgccacc gagcgtggaa aggtcctctt cattttaagc tattctggga
     3541 cccagccgga tgagtgatcg agtgcaggtc gtcgatgagc gaatcaatct tgcgcagatc
     3601 tttgcgacgg cctttttcgg gtttcaattt gcgcttgcgt accatgatgg tgaggtcgcg
     3661 aatatcgtgc aattgctccc gcgacaatgc ttctgctctt ttcgaggaaa cggccgccac
     3721 gctttccgcc agggcgactt cgagccgtga cgcgtacgct cgaatcgttt tccgcaagtc
     3781 acgccgaaga acggccagtt ctcctgcaag cattgtcacg ggagtg
//
LOCUS       DASQUQ010000223         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_593440, whole genome shotgun sequence.
ACCESSION   DASQUQ010000223 DASQUQ010000000
VERSION     DASQUQ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593440"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..279
                     /locus_tag="VE486_06345"
     CDS             103..279
                     /locus_tag="VE486_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38743.1"
                     /translation="MALLRGLFWLVLFIVLAFSFVVLFEYGPHHFASGFQKEFARAKK
                     FVVKQTEKIQKPKK"
     gene            complement(432..1328)
                     /locus_tag="VE486_06350"
     CDS             complement(432..1328)
                     /locus_tag="VE486_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HZA38744.1"
                     /translation="MATERGVFTLGHSPDPDDAFMFYAMAANKIDLRGYRFEHRLEDI
                     QTLNERALRGELHMSAISIHAYAYVADKYALLPCGASMGDGYGPIIIRKRRTSNIERR
                     TSDESLAFDESAAREFFGGKKIAVPGAMTSAFLALQLFLGEFDYVVVPFDQIFEAVRE
                     GRADVGLIIHEGQLTYERSGFEKVIDLGEWWKRETSLPLPLGGNVLRKDIPRPIQRDL
                     LEIIRESIEFGLAHRDEAVSHSLSYARDMNADLASKFIGMYVNEFTRDYGEIGRTAIR
                     KFLGAARDKGYINGPIDVEFIE"
     gene            complement(1608..2606)
                     /gene="hflC"
                     /locus_tag="VE486_06355"
     CDS             complement(1608..2606)
                     /gene="hflC"
                     /locus_tag="VE486_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HZA38745.1"
                     /translation="MNKRGCFWLLLIALAFAVLSTLLGCFYVVHQTEQVIITQFGKPV
                     GEPITKPGLHVKLPIVQEVNRIDKRFLEWGGAPVAIPTRDKTYIHVDTFARWRISDPT
                     TFFVRLRDERSAQSRLEDILGSETRNAVAKHDLIEIVRTDKERKPLRDETLKNVPTGL
                     GTIGVLPPIHYGRVKIEEEIKTKGAKKLAEFGIDLLDVRLKRVNYNPDVLDRIYQRMI
                     SERRQIAQRFRSEGEGEAARIAGQKERDLNEIQSTAYRQVQQIRGEADAKATEIYALA
                     YTQNPNAADFYNFLKSMETYRKVMAKDATLILSTDSDLFNLLKRASAKPSNAPPVR"
     gene            complement(2603..3586)
                     /gene="hflK"
                     /locus_tag="VE486_06360"
     CDS             complement(2603..3586)
                     /gene="hflK"
                     /locus_tag="VE486_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HZA38746.1"
                     /translation="MIDRPPSALETFQMPHIPVRAIGAIVAIILLIVLTWTSCYTVQA
                     ESEGVVLRFGKFLKTVEPGLHFKLPFGIDHVSVLPTRRQLKLEFGFGTPEYTNPDQPG
                     GDVQEERSMVTGDLNAALVEWVVQYRIDDPKQYLFEVRNPGQTLRDLSEAAMREVIGD
                     RTVDEVITIGRQDIEISALARMQELAKRYMLGVRVDQVQLKNVNPPAEVQASFNEVNK
                     AQQDRENAINIANGDYNEAVPRARGQADQMIRGAEGYRFKRVNEAQGDVASFNAILAE
                     YVKAPEITRTRLYLETMADVLPATGEKIIIDDSMRNLLPILPLNAKPLEKR"
     gene            3827..>4159
                     /locus_tag="VE486_06365"
     CDS             3827..>4159
                     /locus_tag="VE486_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HZA38747.1"
                     /translation="MARSGLGKGLGALIGTPASTPRADVADSSERIHQVDLANILPSA
                     LQPRKDFARDALQELIDSIRQHGIIQPLIVRLAAASPSSGGQAGGRFELIAGERRWRA
                     AQEIGIPQV"
BASE COUNT          857 a         1177 c         1129 g          996 t
ORIGIN      
        1 caagcatctt gtccgccacc ggacggactc ggtgcggcga gcttcgtcgg atagccttcc
       61 gcagcgttga ccctcacctc gggctgatta ctctgtctga tcatggcact tctgcgtggt
      121 ctcttctggc tcgttctttt cattgttctc gctttttctt ttgtcgttct gttcgagtac
      181 gggccgcatc acttcgcgag cggctttcaa aaagaatttg cccgcgcaaa gaagttcgtc
      241 gtgaagcaga cagaaaaaat ccagaaaccg aagaaataac cgctgacaag tcgtcgcaaa
      301 ttgtagtctt caccgcggcg cgagagagga gcagttcctt ctccctggga gagaaggtta
      361 ggatgagggc tccttgcgag ttgctggttc cgctgcgacc gcaaattccc acctgtgcaa
      421 gccgtgttgt atcattcgat aaattccacg tcgatcggac cattgatata gcctttgtcg
      481 cgcgcggcgc ccaggaactt gcgaatggcc gtgcgtccga tctcaccgta atcgcgagta
      541 aattcgttca catacatccc aataaatttg ctggcgaggt ccgcattcat atcacgcgca
      601 taagaaaggg aatggctcac cgcctcatcc cgatgcgcca ggccgaactc gatgctttcc
      661 cggataattt cgagcaaatc gcgctgtatt ggacgcggaa tatccttgcg caagacgttg
      721 ccgccgaggg ggagcggcaa gctcgtttcc cgtttccacc attcgccgag atcgatcact
      781 ttttcgaagc ccgaccgctc gtaggtgagc tgaccttcgt gaatgattaa gccgacatcg
      841 gcccgcccct ctcttaccgc ctcaaagatt tgatcgaacg gcacaacgac gtaatcgaat
      901 tctccaagaa aaagttgcag cgcaagaaac gcgctcgtca tcgcccccgg caccgcgatt
      961 ttcttgccgc caaaaaactc tcgggctgcc gattcgtcga acgcgaggct ttcgtccgat
     1021 gttcgacgtt cgatgttcga tgttcgacgt tttcggataa tgatcggtcc atatccatcc
     1081 cccatgctcg ccccgcaggg cagcagtgca tacttgtccg ccacatacgc gtaggcgtga
     1141 attgagatgg cagacatatg aagctcgccc cggagcgcgc gttcattcag cgtttggatg
     1201 tcctccagcc gatgttcaaa tcgataaccg cggagatcga tcttgtttgc cgccatcgca
     1261 taaaacatga acgcgtcgtc cggatcaggc gaatgaccca atgtgaacac accgcgctcc
     1321 gttgccatgg ccggacgcta tctgtttcgg ttgtcagcgc aatgctgcgg gagctgagtg
     1381 ttcgttcaaa atgtcaaaac ttcagacgcg atccgccatc gttctctcct gcgcgcgggg
     1441 aaaggacgat cctgtaggca aaaaacagta accagtcggc aaccaccggg ttgccactcc
     1501 gtgagaggta accggagcga aaaggtcatc tccccggatg gtaatcgcga tttggagcaa
     1561 actattaccg tccattctta gcagcaaata ccggccggcc ggtgcggtta gcggacaggt
     1621 ggcgcgtttg aaggttttgc ggacgcgcgc ttcagcaagt tgaagagatc gctgtcggtg
     1681 gaaaggatca gtgtcgcatc cttcgccatt accttgcgat aggtctccat gctcttgagg
     1741 aaattgtaga agtcggccgc attcgggttc tgggtgtaag cgagcgcgta gatctcggtc
     1801 gctttggcgt cggcttcacc acggatttgt tgaacctgcc ggtaggcggt tgattgaatt
     1861 tcatttagat cgcgttcctt ttggccggcg atgcgcgccg cttcgccttc accttccgag
     1921 cgaaaccgct gcgcgatttg ccggcgttcg ctgatcatgc gctgatagat tcgatcgaga
     1981 acatcgggat tgtaattcac ccgcttgagc cggacgtcga gcagatcgat accgaattcc
     2041 gccaatttct tcgcgccctt cgttttgatt tcctcttcga ttttcactcg tccgtagtga
     2101 atcggcggga gcacgccaat cgttcccaaa ccggttggca cattcttcag agtttcgtcc
     2161 cggagaggtt tgcgttcctt gtccgtgcgc acaatctcga tcaaatcatg cttcgccacg
     2221 gcgttgcgcg tttcgcttcc aagaatgtct tccaaacgcg attgggcgct tcgttcatcg
     2281 cgcagccgca cgaaaaaggt tgtgggatcg gagatgcgcc agcgagcaaa agtgtcgacg
     2341 tgaatgtaag tcttgtcccg tgtcgggatg gcaacgggcg cgccgcccca ttcgagaaaa
     2401 cgcttatcga tgcggttgac ctcttgaacg atgggaagct tcacatgcag ccccggtttc
     2461 gtgattggtt ctccaactgg tttgccgaat tgcgtaatga tcacctgttc ggtttggtga
     2521 acgacgtaga agcatcccag caatgtggac aaaaccgcga acgccagcgc gattaacaac
     2581 aaccaaaagc acccgcgctt gttcatcttt tctccaacgg tttcgcgttg agcgggagaa
     2641 tcggcagcaa gtttcgcatt gaatcgtcga tgatgatctt ctcgccggtc gcggggagca
     2701 cgtccgccat cgtttcgaga taaagccggg tgcgcgtgat ttcgggcgcc ttaacgtatt
     2761 ctgcgagtat ggcattgaac gatgcgacgt cgccctgcgc ttcattcacg cgtttgaagc
     2821 gatagccttc ggccccacga atcatctggt cggcctgccc ccgcgctcgc ggcacggctt
     2881 cgttgtaatc gccgttagcg atgttaatcg cgttttcgcg gtcctgttgc gctttattta
     2941 cctcgttaaa ggacgcctgc acctccgccg gcgggttgac gtttttcagt tgtacttgat
     3001 cgacgcggac cccgagcatg tagcgcttgg ccaattcctg cattcgcgcc agcgccgaga
     3061 tctcaatgtc ctgccggcca attgtgatga cttcgtccac ggtgcggtct ccgatcactt
     3121 cgcgcatcgc tgcctccgat aaatcgcgca aggtctggcc aggattgcga acctcaaaga
     3181 gatattgctt cgggtcgtcg atgcgatatt gcactaccca ttcgacgagc gcggcgttca
     3241 ggtcgcctgt aaccatcgat ctttcttctt gcacatctcc ccccggctgg tcgggattcg
     3301 tatattctgg cgtcccgaag ccgaactcga gtttcaattg ccgtcttgtt ggcagaacgc
     3361 ttacatgatc gatgccgaac ggaagtttga agtgcaaacc gggttcgacc gttttcaaaa
     3421 acttgccaaa acgaagaacc acgccttccg attccgcctg tacggtgtag caagatgtcc
     3481 aggtaaggac gatcagtaga atgatcgcca cgatagcgcc gatcgcccgc accggaatgt
     3541 gtggcatctg aaaagtctca agcgcggaag gtggtctatc aatcatcgtg tctcattagg
     3601 aatgcacctg ccggaagcgt agacagatcg ctccattcgg gcaaagcgca acatgaacgc
     3661 gtcgtcgaga tcaggtgaat aacccaatgt aaaaacaccg cgccccgtcg ccatggccgg
     3721 acgctacccg tttcgctgct tggcgcaacg aagcggtact tttgctgttc ccctttttcg
     3781 attcttcggt ttgcgttagc ggggctgttc cggtagcctc ccgcgcatgg cgagatccgg
     3841 tcttggtaaa ggtcttgggg cactcattgg cactcccgcg agcactccac gggcggatgt
     3901 cgcggactcc agcgaaagga ttcaccaggt tgatctcgca aatattctcc cgagcgcatt
     3961 gcaaccgcgc aaagatttcg cgcgcgatgc gttgcaggaa ttgatcgatt caattcggca
     4021 acacggcatc attcaacccc tcatcgttcg gctcgccgca gcgagtccgt ccagtggcgg
     4081 acaggctggc ggtcggtttg aattgatcgc aggagaacgc cgttggcgcg cggctcagga
     4141 gatcggtatt ccgcaggtg
//
LOCUS       DASQUQ010000224         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_594006, whole genome shotgun sequence.
ACCESSION   DASQUQ010000224 DASQUQ010000000
VERSION     DASQUQ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594006"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VE486_06370"
     CDS             complement(<1..326)
                     /locus_tag="VE486_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZA38748.1"
                     /translation="MDYAIRPLTTADEPILWETLYQALHTSEGGPPRDAVKRPEFARY
                     VDGWGRPDDAGFVAHDKESGQVLGAVWIRRLSDQRDAPPDLAFGVKPGLRRRGIGAAL
                     LTQLVKA"
     gene            complement(399..878)
                     /locus_tag="VE486_06375"
     CDS             complement(399..878)
                     /locus_tag="VE486_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZA38749.1"
                     /translation="MNTSTPTTIDQSKPADVVVRGNARGFLQEVVNGKHTLRADEPVS
                     AGGTDAAPSPYDYLLIALGVCTSMTVGLYARRKQWPLENITVSLRHSRIHAKDCEECE
                     TKEGMLDRIDTEIELTGSLTGEQRAKLMEIAAKCPVHRTLKSEINIRLQAAEKSSAS"
     gene            complement(885..1259)
                     /locus_tag="VE486_06380"
     CDS             complement(885..1259)
                     /locus_tag="VE486_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HZA38750.1"
                     /translation="MKILAIIARILLGLIFLVFGSNAFLNFIPMPPPPPGLAGDYTKV
                     FLASGYAHFIGGLQVIAGLLLLIGRFVPLGLTILGGIIVNIWAFHLLMLPEGLPPAIV
                     VTVLELFLIWRYREAFAGLVRP"
     gene            1504..3129
                     /gene="murJ"
                     /locus_tag="VE486_06385"
     CDS             1504..3129
                     /gene="murJ"
                     /locus_tag="VE486_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZA38751.1"
                     /translation="MAAAASQRISTRATGVVGIAILCSRVLGLIREVVFAAMFGATRN
                     MDAFLTAFRAPNMLRDLFAEGALSTAFVTTFSRRIATKGNQSAWDLASKVATLALIFM
                     SAVSLLGVVFAPVLINILAPGFPLEKAELTVLLTRIMFPFILLVSLAALVMGMLNARH
                     VFGVPALASSFFNLGSIIGGVALCYWLDPQPDWRHPHFGKRGLIGLSIATLIGGLLQL
                     AVQLPSLRRVGFRFHFDFKWRDPGVRTILGMMGPAMIAASAVQVNVAVNSIFASGMGD
                     GPITWLNIAFRLMQLPLGIFGVAVATVTLPLVSRSAAVGNTDEFRSALAHSVRLVLLL
                     TIPAAIGLIILAEPIIHLIYQHGRFTHEATIQTAAALRFYAIGLAGYSADKVLAPAFY
                     ALDKRHLPMFVSLTSIAVNFSLNWFFTFHLHLGHRGLALSTSLVAVTNFFFLYSMMRY
                     YTGHLETGAMLKTIGKLLVAGIGLAAICWLALTFFFAVRPHASDWQNLLVMLITICSG
                     AGIFFGGAYLLHVAEVHDVVELVRRKFGNQPSA"
     gene            complement(3114..>3902)
                     /locus_tag="VE486_06390"
     CDS             complement(3114..>3902)
                     /locus_tag="VE486_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZA38752.1"
                     /translation="FLRWGLEADFIDMSNLEEVKRALRPQTKLAWAETPSNPLLKIVD
                     LAAVAELVHGAGALCVCDNTWAPILQRPFDLGADLILHSTTKYFGGHCDVAGGILVAK
                     NDTEFFQRVRAIQYSGGAVPSPFDCWLILRGMRTLPWRMRAHSENGMKVAESLAQHRK
                     VKDVHYPGLHSHPGHEIARRQMSEFGGMLSFQVKDGHDAAMSVAAKTKIFTRATSLGG
                     VESLIEHRASIEGPGTTSPEGLLRLSIGLENADDLIEDLDQALG"
BASE COUNT          759 a         1119 c         1097 g          927 t
ORIGIN      
        1 gctttcacca attgggtgag aagtgcggcg ccgataccgc gccggcgcaa accgggtttg
       61 acgccaaagg cgaggtccgg tggcgcgtcc cgctgatcgc tcagccgtcg aatccaaacg
      121 gcgccaagca cctgaccgct ctctttgtca tgcgcgacga agccggcatc atccggacga
      181 ccccagccgt caacatagcg cgcgaactcc gggcgtttca cagcgtctcg cggcggtcca
      241 ccttcggagg tgtggagcgc ttgatagagc gtctcccaca ggatgggttc atccgcggtt
      301 gtgaggggac gaattgcgta atccatggcg aagagttgtg actccgaata tacaccaaac
      361 ccggccagcc gcggccaatc gcgcgatgcc ggtccaattc acgaagcgga tgatttctcg
      421 gcagcttgga gtcggatgtt gatctccgac ttcagcgttc gatgaacagg acatttcgcg
      481 gcgatctcca tcagtttcgc ccgttgctcg ccggtcaatg agccggtcaa ctcgatttct
      541 gtatcgattc gatcaagcat gccttccttc gtctcgcact cttcgcaatc tttcgcgtgg
      601 atgcgcgaat gccgaagcga cacggtgata ttttccagcg gccattgttt ccgccgcgcg
      661 tataagccga ccgtcatcga tgtgcagacg ccgagcgcga tgagcagata atcgtacgga
      721 ctcggcgcgg catcagttcc gcctgcgctc accggctcat cagcgcgtaa ggtgtgtttc
      781 ccgttgacaa cctcctgtag aaatccgcgg gcgttgccgc gaacgacaac gtccgctggt
      841 ttgctttgat cgatcgtggt gggagttgac gtgttcatca gcagttacgg tcgcaccaat
      901 ccggcaaatg cctcgcgata tcgccaaata agaaacagct caagcacggt gacgacgatc
      961 gcaggtggca gcccctcggg cagcatcaga agatgaaacg cccaaatgtt tacaatgatg
     1021 ccgccgagaa tcgtcaggcc gagcggtaca aaccgaccga tcaggagaag caagccagcg
     1081 atgacttgca gcccgccaat gaagtgagcg tagccgctgg ctagaaatac tttcgtgtag
     1141 tcaccggcca gccctggtgg gggtggcggc atgggaatga aattgagaaa cgcgttcgat
     1201 ccgaacacga ggaaaataag tccaagaagg attcgagcaa taattgcaag gattttcatg
     1261 caaggacggt ggcttgatcg agccgaaatt ccaatcccta agtttctcga cgtttgcttt
     1321 agttttcaga acggcggtga cggcaatgat taaatttcca aagtcgccgc catttgcgca
     1381 gctgaatgac gcatgtcgcc tgcagagctt ttgattgggc gtgagacaga cgccctccag
     1441 ttctggcaga tcgcgcgaag ttttttcttt cgtaaatcct gagagtctgt tctttgttcg
     1501 ccgatggctg ccgcggcaag tcaacggatc agcacgcgcg cgaccggtgt tgtcgggatc
     1561 gcgattcttt gcagccgcgt actcgggctg attcgggaag tagttttcgc ggcgatgttc
     1621 ggcgcgactc gcaatatgga cgcgtttctg accgcgtttc gcgcgcccaa tatgttgcgc
     1681 gatctctttg ccgaaggcgc gctttccacc gcgtttgtga ctacgttctc gcgccggatt
     1741 gcgacgaagg gcaaccaatc agcctgggat ctggcgagca aggtagcaac gctcgcgctc
     1801 attttcatga gcgcagtttc gctgctcggc gtcgttttcg caccggttct tatcaatatt
     1861 ctcgcgcccg gtttcccgct tgaaaaagcc gagctcaccg tgctcctcac gcggatcatg
     1921 tttccgttta ttctgctggt ctcgctggct gcgctggtga tgggaatgct aaacgcgcga
     1981 cacgtatttg gcgttccggc gttggcttcg agttttttca acctcggctc catcattggc
     2041 ggggtagcgc tctgttactg gctcgatcca cagcccgatt ggcgtcatcc gcatttcggc
     2101 aaacgcgggc taatcggtct gtcgattgca acgttgatcg gcgggttgtt gcagttggcc
     2161 gtgcaattgc cgtcgcttcg tcgtgtcgga ttccgctttc attttgactt caaatggcgc
     2221 gacccgggcg tgcgaaccat tctcggaatg atggggccgg cgatgatcgc cgcgagcgcc
     2281 gtgcaggtga acgtagcggt caactccatc ttcgcatcgg ggatgggtga tggaccgatc
     2341 acgtggctga acatcgcctt tcgtttgatg caattgcctc tgggaatttt cggcgtggcg
     2401 gtcgcgacag tgacgctgcc gctggtttcg cgcagcgcgg cagttggcaa tacagacgaa
     2461 tttcgctctg cgcttgcgca ctcggttcga ctcgttctgt tgcttacgat tcccgctgcg
     2521 atcggtctaa tcattctggc ggaaccgatc attcatttga tttaccaaca tggtcgcttc
     2581 acgcacgaag ccacgatcca aaccgcggct gcgctccgtt tttatgccat cggtctcgct
     2641 ggatattccg cggataaagt gctcgcgccg gcgttttacg cgctcgacaa acgccatctg
     2701 ccgatgttcg tcagcctgac ttccatcgcc gtcaattttt ctctgaattg gtttttcact
     2761 tttcatcttc acctggggca tcgcggcctc gcgctttcaa ccagccttgt cgccgtcact
     2821 aatttcttct ttctatattc gatgatgcgc tattacacag ggcacctcga gactggcgcg
     2881 atgctcaaaa cgatcggcaa gttgctcgtc gccggcatag gcctggccgc catctgctgg
     2941 ctggcgttga cgtttttctt tgccgtgcgc ccccatgcct cggattggca gaacctgctt
     3001 gtgatgttga tcacgatttg ctccggagcg ggaatatttt tcggcggcgc atacctgttg
     3061 cacgtcgccg aagtgcacga cgttgtcgag ctggtgcgtc gcaaattcgg gaatcaaccc
     3121 agcgcttgat ccaggtcctc gatcaaatcg tccgcatttt cgagaccgat cgataatcgc
     3181 aacaggccct ccggcgaagt tgtcccgggc ccctcaatcg atgctcgatg ctcgatcaag
     3241 ctttccacgc cgcccaggct ggtcgcgcgg gtaaagattt tcgttttcgc cgcaactgac
     3301 atcgccgcgt cgtgcccatc tttgacctgg aaggaaagca ttcctccgaa ctccgacatc
     3361 tggcgcctgg cgatttcgtg tcccgggtgg gaatgcagtc cgggataatg cacgtcctta
     3421 acctttcgat gttgagccaa ggattccgcc accttcatcc cgttttcaga gtgcgccctc
     3481 attcgccacg gcaacgtgcg cattccccgg agaatcagcc agcaatcgaa gggcgagggg
     3541 actgcgccgc cggaatattg gatagcccga acgcgctgaa aaaattcggt gtcattcttt
     3601 gcgacaagaa tcccgccggc tacgtcgcaa tggccgccga aatatttcgt ggtcgaatgt
     3661 aaaatcagat cggcgcccaa atcgaaggga cgctgtaaaa tcggcgccca ggtattgtcg
     3721 caaacgcaga gcgcgccggc gccgtgaacg agttcggcga ccgccgcaag atcgacaatt
     3781 ttcaatagtg gattggatgg tgtctcggcc caagccagct ttgtttgcgg ccgcagcgct
     3841 cgtttcactt cttcgagatt gctcatgtcg atgaagtccg cttccaatcc ccagcgtaag
     3901 aa
//
LOCUS       DASQUQ010000225         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_149274, whole genome shotgun sequence.
ACCESSION   DASQUQ010000225 DASQUQ010000000
VERSION     DASQUQ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149274"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1282)
                     /locus_tag="VE486_06395"
     CDS             complement(89..1282)
                     /locus_tag="VE486_06395"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HZA38753.1"
                     /translation="MSTKKGVIGILTGGGDVPGLNPAIRAVTVRALREGYQVVGIRHG
                     WLGAISILRDEHADNSEHFQILTEEIVNRAARTGGTFLHTSRTNPSVVKKEEVPEALR
                     ATFNEAKNDLTSEVIKNLDWLGIDYLIPIGGDDTLSFATRLSKEGVKVVAIPKTMDND
                     VPGTDYCIGFSTCVSRTIELSNRLRTSAGSHERFLVMEVFGRYAGFTAMLPTMAGAAN
                     RCVIPEYKFDMEHLTELLCYDRAHHPSKYSVVIVSEGAMFEGGEMVFSDRTTDAFGHL
                     KLGGIGDLVSAELKERSAKYNKGKSIQTINQRLGYMVRGGDPDAIDSIVPMAYGNLAL
                     DLILHGHHGRLVVLKNGRYDNMPIEAVTSTKKTVNVDKYYNKERLRPLYTDFEMQPLF
                     IMASD"
     gene            complement(1613..2068)
                     /locus_tag="VE486_06400"
     CDS             complement(1613..2068)
                     /locus_tag="VE486_06400"
                     /inference="COORDINATES: protein motif:HMM:NF023583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HZA38754.1"
                     /translation="MANRNSKNAPAPKLETVLWQAADKLRGNLDAAEYKHVVLGLIFL
                     KYVSDAFEEKHAQLAKETGADPEDRDEYLAENIFWVPPEARWSYLRGRAKDPLIGKLI
                     DDAMVAIERDNTSLKSVLQSGHDGSLRVAARGDSGRRRLRVRCHTASRS"
     gene            complement(2288..2827)
                     /locus_tag="VE486_06405"
     CDS             complement(2288..2827)
                     /locus_tag="VE486_06405"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HZA38755.1"
                     /translation="MIRTIRGVRVMLDRDLARIYGVPTFRFNEAIKRNRDRFPADFMF
                     QLTREEFDSLRSRIAMSKRKNSSQIAMSSGKHRGAAYRPYAFTEHGALQAANVLRSSR
                     AVQMSVFVIRAFVKMREALRRTPELARKLAALEKKLTARLDVHETAIVQVLQEVMQIL
                     NPTSPPPEPPRRRIGFHES"
     gene            3235..3453
                     /locus_tag="VE486_06410"
     CDS             3235..3453
                     /locus_tag="VE486_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38756.1"
                     /translation="MSRVEEIKQAIETLSPEERCQLDALLNPIEDDDWDRQMKKDTEP
                     DRKLDRLKETATKEYKQGKSLPFPKPAE"
     gene            complement(3733..>4827)
                     /locus_tag="VE486_06415"
     CDS             complement(3733..>4827)
                     /locus_tag="VE486_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38757.1"
                     /translation="EGSDPQDEFRGKNILIQRHTIAETAKQFRNTGILSASRTDSSGG
                     EPVRPAGVSPADKDLENAVRDLLKRSREKLFAIRAKRPRPHLDDKIIAAWNGLMISAY
                     ARAAQALGEPRYLEIATRAAKFLRANLYDEKSKLLFRSYREGRGAVEGFADDHAFVVQ
                     GLLDLYEATFDVEWLKFAIELQETQDRLFFDEKNGGYFNTSGKDKSVVLRMKDDNDSA
                     EPAASSIAALNLLRLSQIADRKEWQERAQKTIAAFGPTLSHFASAMPQMLVALDYSLS
                     KPRQIVIAGKKDASGTKALVAEVHRHFLPKTTLFLADGAEGQKYLGEKNEAIRAMSMV
                     DGKPAAYVCENFTCKTPVTDPKALAELLSR"
BASE COUNT         1097 a         1268 c         1343 g         1119 t
ORIGIN      
        1 ggcgtgcctg ccatgccaaa gcgttaccta gggtaggttc gcctgcgatt cttgttgtag
       61 cggcttagcg aggcccgcgc ggcttttgct aatcactcgc catgatgaat agcggctgca
      121 tttcgaaatc ggtgtagagt ggacgcaggc gctccttgtt atagtatttg tccacgttga
      181 ccgttttctt ggtgctggtc accgcttcaa tcggcatgtt gtcatagcgt ccattcttca
      241 agacgacgag gcggccgtgg tggccatgca ggatgagatc gagggcgagg ttgccgtagg
      301 ccatgggcac gatcgaatca atcgcgtcgg gatcgccccc gcgcaccata tagccgagac
      361 gctggttgat ggtctggatc gacttgccct tgttgtattt ggcggaacgc tccttgagtt
      421 cggctgagac caggtcgccg attccgccga gtttcagatg accgaacgcg tcggtcgtcc
      481 tgtcggagaa caccatctca ccgccttcga acattgcccc ttctgaaacg ataacgactg
      541 aatatttgct cggatgatgc gcgcggtcat agcacaacaa ctcggtcaga tgctccatgt
      601 cgaatttgta ttcggggata acgcaccgat tcgccgcgcc cgccatcgtc ggcaacatcg
      661 cagtgaaccc cgcgtagcgt ccgaacactt ccataacgag aaatcgctcg tgagatccgg
      721 ccgaggtgcg gagacgattc gacaattcaa tcgtgcggct gacgcaggtg ctaaagccga
      781 tgcaataatc ggtgccgggc acgtcattgt ccatcgtctt gggtatcgcc acgaccttga
      841 cgccttcctt cgacaaacgg gtcgcaaaag aaagcgtgtc gtcgccgccg atcggaatca
      901 gatagtcaat tcccagccaa tccaggttct tgatgacttc agaagtgaga tcgtttttgg
      961 cttcattgaa ggttgcgcgc agcgcttcag gtacctcttc ttttttcact actgacgggt
     1021 tcgtccgcga ggtatgcaga aacgtaccgc cggtgcgtgc tgcacgattg acgatctctt
     1081 ccgtcaggat ttgaaagtgc tcgctgttgt cggcgtgttc atcgcgcagg atgctgatcg
     1141 cgcccagcca gccatggcgg ataccaacga cctgatagcc ttcgcgcagg gcgcgaacgg
     1201 tgaccgcgcg gatcgcggga ttgagccccg gcacatcccc gccgccggtg agaataccga
     1261 tcacgccttt cttggtgctc atgcaacccc tttgtcgttt tcaatatccg gcatgattgt
     1321 gtggcgcaac tcgctttgca caacgaagcc gcctcaactc tcaactagag acctcaacta
     1381 attgaattgc gacatggccg gtagagccgc aaagataggg gtcgagcgcg gcgagttcgc
     1441 cgcgaggaga gcttaggccg cgtgctactt cacgttgacg gcaatagcga tcattggcgt
     1501 ggggaagaca tgctcatggt gagggtcgat tacaccactc gggaggaagt accatttgcc
     1561 gggggccatg ttgcccagag ctggaagcct tggcaggcgc tgggggtcga ggctagctgc
     1621 gagaagctgt gtggcatcga actcgtagac gacgtcttcc gctgtcccct cgggctgcaa
     1681 cgcgtagtga accatcgtgc ccggactgca acacgctttt gagcgaagta ttgtcccgct
     1741 cgatcgcgac catcgcgtcg tcgatcagct ttccgatcaa tggatctttc gcacgcccgc
     1801 gaagataaga ccagcgggcc tctggcggca cccaaaagat attctcggcg agatactcgt
     1861 ctcggtcttc tggatcagcg cctgtttctt ttgcgagctg cgcatgtttt tcctcaaatg
     1921 cgtccgagac gtattttagg aaaatcaatc caaggacgac gtgcttgtat tctgccgcgt
     1981 cgagattccc gcgcagctta tccgcagctt gccacagaac agtctcgagt ttaggagccg
     2041 gcgcgttctt cgaattacga ttcgccataa gagcgcggaa aatagcacaa aatgggatga
     2101 aagtcagccg ccttcattaa aattacggcg agccaaggaa gatccgcggt ggctaggggc
     2161 ggaacgaagc cagaaggcaa attaagaatt atgaaaaagg gaagcgcggg aagttggaag
     2221 tcagaattaa gaaataagag cgtaaagctc cgtgggagcc acgctccttt acggagttga
     2281 gagcgattca ggattcgtga aaaccgattc tccgccgtgg cggctcgggc ggtggtgatg
     2341 tcggattgag gatctgcatg acctcctgca ggacttgcac gatcgcagtt tcatgaacgt
     2401 ccaagcgggc agtaagcttt ttctcaagcg ccgcaagttt ccgcgcaagc tccggcgtgc
     2461 ggcgaagcgc ttcgcgcatc ttgacgaaag cgcggatgac gaaaacactc atttgcacgg
     2521 cgcgcgagct gcgaagaacg tttgctgcct gtaatgcgcc gtgttcagtg aacgcgtagg
     2581 gccggtaggc ggctcctcga tgcttgcctg aactcatcgc aatttgcgat gagttctttc
     2641 gttttgacat cgcaattcgc gatcttaaag agtcaaactc ctcacgcgtg agttgaaaca
     2701 tgaaatcggc tgggaaacga tcgcgattgc gtttgatggc ttcattaaag cgaaatgtgg
     2761 gaacgccgta gattctcgcc aggtcacgat cgagcattac tcgcacgccg cgaatagtgc
     2821 gtatcatcgc gtcaatctcg tcgaccgtag caggggattt ggactttctc ggcataagcg
     2881 caggttcagc aattcaaggt cacaatttgt gacctcaagt tgtgcttctc ctcgatgtcg
     2941 gcatgggagc gcggagaata gcagaaactc gaataagtca gaagtaaaaa ttaaggatta
     3001 gaaaacgggg agcacgggac gttgaaagtc agccgccttc attaaaatta cggcgagcca
     3061 aggaagatcc gcggtggata aggccggaac gaagccagaa ggcaaattaa gaattatgaa
     3121 aggggaagca cggcaaattg gaagtcagaa ggttttccat caaccatcga gatttttggg
     3181 cgaatgactg ctcatttctt gtccaaatct ttattcgggg tacactctat gggcatgagt
     3241 cgggtggaag agatcaagca ggccatcgag acgttgagtc cagaggaacg ctgtcaactc
     3301 gatgctctgc tgaatcctat cgaagatgac gattgggacc ggcaaatgaa aaaggatacc
     3361 gagccggatc ggaaactgga tcggctcaag gaaaccgcaa ccaaggaata caagcaaggt
     3421 aagagcctgc cgttcccaaa gccagccgag tgaagtccaa aacttcgcgc gaattttgga
     3481 aattgtttca caggcttccg gtcgatattc aaaagctggc ttacaagaat tatctctttt
     3541 ggcaaaacga tccgtgacat cgatcgttgc gttttaaacc gattaaatcg aagcaatggt
     3601 cagtgcgagt gggcgagcac taccgcgccg taggccattt cgttgagaaa gacacgtttc
     3661 tttggacatg gatcggaacc caggaggatt acaataaatt ctgaatcttt ctacgttcga
     3721 cagtgcctca tcctaccgtg acagcaactc ggccagtgct tttggatcgg tcacgggcgt
     3781 tttacaggtg aagttttcgc atacgtaagc ggccggtttt ccatcaacca tcgacatcgc
     3841 gcgaatcgcc tcgttttttt cgccgagata tttttgtcct tcagctccgt cggccagaaa
     3901 caaggtcgtc ttcggcagaa aatgacgatg cacttccgcg accagcgctt ttgtgcccga
     3961 cgcatccttt tttccggcga tcacgatttg tcgcggcttg cttaacgagt agtcgagcgc
     4021 taccagcatt tgcggcatgg cactggcaaa atgcgaaagc gtcggcccaa acgccgcgat
     4081 agtcttctgc gcgcgttcct gccattcctt tcggtcggcg atttgtgaga gccgaagcag
     4141 attgagggca gcgatggaac tcgccgccgg ttccgcgctg tcgttatcat ccttcatgcg
     4201 aagcacaacg cttttgtctt tcccgcttgt gttgaaatag ccgccatttt tttcgtcgaa
     4261 aaacagacgg tcctgcgtct cctgcaattc gattgcgaat ttcagccact cgacatcgaa
     4321 tgttgcttcg taaagatcca gaagcccttg tacgacgaac gcatgatcat cggcaaaacc
     4381 ttccactgcg cccctgccct ctcgataact gcgaaagagc agtttgctct tttcgtcata
     4441 aagatttgca cgcaggaact tggcggctcg agtcgcgatt tccaggtagc gcggctcacc
     4501 aagagcctgg gctgcgcggg cataggcaga aatcatcagc ccgttccacg cagcgatgat
     4561 tttatcgtcg agatgaggac gcggccgctt cgctcggatt gcgaagagct tttcgcgact
     4621 gcgtttcaat aaatcgcgaa ctgcgttctc taaatcctta tcggcgggcg agacgcccgc
     4681 cggccgcaca ggctcgccac cggacgagtc cgtccgactg gcggacagga tgcctgtgtt
     4741 acgaaattgt ttcgctgtct cggcgatcgt gtgccgctgg atcaaaatat ttttaccccg
     4801 gaattcgtcc tgcggatcgc ttccttc
//
LOCUS       DASQUQ010000226         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_232538, whole genome shotgun sequence.
ACCESSION   DASQUQ010000226 DASQUQ010000000
VERSION     DASQUQ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232538"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VE486_06420"
     CDS             complement(<1..276)
                     /locus_tag="VE486_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38758.1"
                     /translation="MAYKAAPEAFIYPVSRRVLVGTAVFALIVPSTIADTFPVSGPVV
                     SGKKARLYSGQAAAPKKAPAAVKRAIWAANRLCSKPYRYGGGHRAFDD"
     gene            451..1038
                     /locus_tag="VE486_06425"
     CDS             451..1038
                     /locus_tag="VE486_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38759.1"
                     /translation="MTRIFLLLVTAIVAATTALRADQSDQDNSEEPDSFEIEPPILKQ
                     NLSDEPLPDGDVPRLEKQLERAKRNAAGAERLCKIGVLAKVEVEQRLLKVLRCESDLT
                     NARLADAKEQVVEQESRIAAGENAKDELEAAKVALAQLTEAAELASRKRERAELDFAE
                     ANLRRQQKLLRLGSADKSDVNRAQEKLAELKAQTN"
     gene            1043..1366
                     /locus_tag="VE486_06430"
     CDS             1043..1366
                     /locus_tag="VE486_06430"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HZA38760.1"
                     /translation="MKRFYLALCVIGFLLPYSQFVPWVAEHGLNFSLFVHDLFVNRIS
                     GFFGLDVLVSAVVLWIFIVVEGSRLGMGRLWMPIVATCVVGVSLGLPLFLYLRQVHLD
                     RATRE"
     gene            complement(1390..>2358)
                     /locus_tag="VE486_06435"
     CDS             complement(1390..>2358)
                     /locus_tag="VE486_06435"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZA38761.1"
                     /translation="GGFLRKTDFEKHASTWVDPVSANYRGYDVFELPPNGQGIATLQI
                     LNILEGFDLRKMSRNSPDTLHTMIEAKKIAWADRAKFYADPAFAKIPLSGLLSKEYAT
                     ERRKLIDPNRAAKKVEAGNPALDQGDTIYLCTADNEGNMVSLIQSNYRGMGSGIVVPG
                     LGFMLQDRGELFSMEPQHANVYVPGKRPFHTIIPGFVMKDGKPWEAFGVMGGGMQPQG
                     HVQVLTNQIDFGLNVQEAGDASRWQHEGDNEPTGEKMTESGGYVEVESGIPYETVREL
                     RQKGHDVRFGVGGYGGYQAIKVELHDGQRVYVGASESRKDGQAAGY"
BASE COUNT          488 a          655 c          658 g          557 t
ORIGIN      
        1 gtcatcaaac gcgcggtgac cgccgccgta ccggtatggc ttcgagcaaa gtcgatttgc
       61 ggcccaaatc gctcgtttga ctgctgcggg cgcttttttc ggcgcagccg cttgaccgga
      121 atagaggcgc gccttcttgc ccgatacaac cggtcctgac accggaaagg tatcggcgat
      181 tgtcgatgga acaatcagcg caaaaacagc tgtgccgacc aggactctac gactaaccgg
      241 atagataaat gcctccgggg ctgccttgta tgccaaaacg tctgcggttg aaaaggacaa
      301 aattcgagaa attttctggg gattgcttgc atggagcgcc tggaatgaca tgccagggcg
      361 ctgtagtgtc cgctgtcctc agcggacttt gcagcgagtg catcgcagca aatcgtgtta
      421 atgcgtgaca tttcgtggta agctcgcggc atgacccgca tttttttgct gctggtcacg
      481 gcgatcgtag cggcgaccac ggcgctgcgt gcggatcaat cagaccaaga caattccgag
      541 gaacctgatt cattcgaaat cgagcctccg atattaaagc aaaacctctc ggatgaacca
      601 ctgccggatg gcgacgttcc gcgtttggaa aaacagttgg agcgcgcaaa aagaaatgcc
      661 gccggcgcgg aacgtctttg caaaattggc gtgctggcaa aggtggaagt ggagcaacgg
      721 ttgttgaaag ttcttcgatg tgagtccgat ctcacaaatg cgcgacttgc tgatgcgaaa
      781 gagcaagtgg tcgagcagga atcccgtatc gcggctggcg aaaatgccaa agacgaattg
      841 gaagcagcga aagttgcgct ggcgcaatta actgaagccg ccgaactcgc gtcgcgaaaa
      901 cgcgagcgag ccgagctcga tttcgccgaa gcaaacttac gtcggcagca gaaactgctt
      961 cgtctcggca gcgcggataa atccgacgtg aatcgcgctc aagaaaaact cgccgagctc
     1021 aaggcgcaaa cgaattgatt ccgtgaagcg tttttacctc gctttgtgtg tgatcgggtt
     1081 tctgctgccc tactcgcagt ttgttccctg ggtagcggag cacgggctca atttcagcct
     1141 ttttgttcac gatttgtttg tcaatcgaat cagtggcttc ttcggcttgg acgttctggt
     1201 ttccgcggtg gtgctctgga tttttatcgt cgtagaagga tcgcggctgg gaatgggccg
     1261 tctttggatg ccaatcgttg ccacgtgtgt cgtcggtgtt tcactcgggt tgccgctgtt
     1321 cctttatctt cgccaagtcc acctggatcg cgcgacccgc gaataagagg acacccgcct
     1381 caaacgtgat caatatcccg ccgcctgtcc gtctttgcgt gattcactcg cgccaacata
     1441 gacgcgttga ccgtcatgca gctcgacctt gatggcttgg tacccgccgt aaccgccgac
     1501 gccgaaccgg acgtcgtgtc ctttttggcg cagctcgcgc acggtctcgt aggggatacc
     1561 gctctccact tcgacgtagc cgcctgactc agtcattttt tctccggttg gttcgttgtc
     1621 gccttcgtgc tgccatcggg acgcgtcgcc ggcctcctgc acattcaaac cgaaatcgat
     1681 ctggttggtg agaacttgga catgaccttg cggttgcatg ccgccgccca tgacgccaaa
     1741 tgcttcccac ggcttgccgt ccttcatcac gaaaccggga atgatcgtgt ggaatgggcg
     1801 tttccccggc acgtaaacgt tcgcgtgctg cggctccatc gagaaaagtt cgccgcgatc
     1861 ttgaagcata aacccgagcc cgggcaccac gatgccgctg cccatcccgc ggtaattgct
     1921 ctgaatcagg ctaaccatgt tgccttcgtt gtcggcggtg cagaggtaaa tcgtgtcgcc
     1981 ctgatccagc gcgggatttc ctgcttcgac ctttttcgcc gcgcgattcg gatcgattaa
     2041 cttgcggcgt tccgtcgcgt attctttcga aagcaagcca ctcagcggaa ttttcgcgaa
     2101 cgccgggtcg gcataaaatt tcgcgcgatc agcccaggcg attttcttcg cctcaatcat
     2161 cgtgtgcagc gtgtcgggcg aattgcggct catttttcgc aaatcgaatc cttcaaggat
     2221 gttcaatatt tgcagagtag cgatgccctg cccatttggc ggaagctcga acacgtcgta
     2281 gccgcgatag ttcgccgaga ctggatcgac ccatgtcgat gcgtgctttt cgaaatcggt
     2341 tttccgcaga aaaccgcc
//
LOCUS       DASQUQ010000227         6719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_52326, whole genome shotgun sequence.
ACCESSION   DASQUQ010000227 DASQUQ010000000
VERSION     DASQUQ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52326"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VE486_06440"
     CDS             complement(<1..330)
                     /locus_tag="VE486_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38762.1"
                     /translation="MFNALSGAERGAATAIVFIGTFFIALGIGRFLKRRAGVRLGLLF
                     RLFCLTLAFYAAIAVYGVHASWPNHVGAALILLSTALVVALVNRYVWDLYFEKKRQTP
                     IPHFLREL"
     gene            complement(451..810)
                     /locus_tag="VE486_06445"
     CDS             complement(451..810)
                     /locus_tag="VE486_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HZA38763.1"
                     /translation="MSILFFRIAAALCFLAVALGAFGAHSLKSTLETHGMLDVWNKAV
                     LYHFIHAIAMFVLALFGAANRGAWWLLFAGIFLFSGSLYVMALIRQPPDWLGAVTPLG
                     GLCFLAGWAWLVIAPTK"
     gene            complement(839..1714)
                     /gene="ftsY"
                     /locus_tag="VE486_06450"
     CDS             complement(839..1714)
                     /gene="ftsY"
                     /locus_tag="VE486_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZA38764.1"
                     /translation="MNFLQSLAQHFAGKPIDWDELEESLIRADLGVPMTMRIIKTLQD
                     REAWSVMGINDVVKVVREEIARILPANPAPIQPLPGQPKVILLLGVNGTGKTTSTAKL
                     ARYLQQQPVRSASRTDRYAVLLAAADTFRAAAIEQLGIWAERLGVEMVRGQYNSDPAA
                     LCYEAYQAATKRGVQFLLCDTAGRMHTKTNLMAELQKVKRTLGKQDPSAPHEALLVVD
                     ATTGGNGLSQAREFQSAIGLTGLIVTKLDGSGKGGIVVAIQDELGIPTRFVGTGEQLE
                     AFAPFDRQVYLDNLL"
     gene            complement(1888..2325)
                     /gene="nusB"
                     /locus_tag="VE486_06455"
     CDS             complement(1888..2325)
                     /gene="nusB"
                     /locus_tag="VE486_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZA38765.1"
                     /translation="MGKRRRAREAAIQYHFWRDLQHGEGPEHIEDFWEFCPATPRVRD
                     FAQPLIEGMVAHLPEIDERIQRYCENYEFRRISAVDRNILRLAIYEMLYRDDIPPVVS
                     INEAIELAKAFGGAESGRFVNGILDRVKDDLKRPLREAVKKGS"
     gene            complement(2331..2807)
                     /gene="ribH"
                     /locus_tag="VE486_06460"
     CDS             complement(2331..2807)
                     /gene="ribH"
                     /locus_tag="VE486_06460"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZA38766.1"
                     /translation="MSKSDPPRPRVVEGKRTFSIVASQFNARYVQGLVDHAEKELRAF
                     APNATISIQRVPGAFEIPVVVRELASQKKTDAIIACGVILKGKTDHAQNLSRSVTDAL
                     QRIAVDHAVPVINAVLSFDDETQACERCLENKINRGTGAARAAVEIARVMSELRGE"
     gene            complement(2942..4159)
                     /locus_tag="VE486_06465"
     CDS             complement(2942..4159)
                     /locus_tag="VE486_06465"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZA38767.1"
                     /translation="MITKTKDTFQFDTIDDVVSDIAKGRIVIVTDDADRENEGDLVMA
                     AEKVTPEAVNFMATHGRGLICAPVSNERAEQLGLQRMVAQNREMYRTDFTVSVDAARG
                     VTTGISAYDRATTILAIANSKSSPEDLAQPGHVFPLRAKDGGVLRRAGHTEAAVDLAR
                     MAGLQPAGVLCEILHDDGTMARLPELMEFRRRHGLRICTIQSLIAHRRVREKLVEPEQ
                     IVKMPTDYGDFDLHLYRSKLDGQHHLALVKGSIDKSKPTLVRVHSQCLTGDVFGSRRC
                     DCGNQLHAALQQIQEEGSGVLVYMRQEGRGIGLAAKIHAYKLQEEGLDTVEANARLGY
                     PSDLRDYGLGAQILFDLGVRRFRFLTNNPKKVVGLEGYGLEMVEQVPIRSEANPHNVK
                     YLETKRSKMGHLL"
     gene            complement(4324..5193)
                     /locus_tag="VE486_06470"
     CDS             complement(4324..5193)
                     /locus_tag="VE486_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38768.1"
                     /translation="MRWLVAFLLFIALAICRATPNPFGVTDESEARPGSATPATKAPV
                     DQLIPWLFDEDRQLRGIAFKEVIFDATGKRVLAVDPKNEIDQRVIKQISAACDETMKR
                     LNAPDSAIQNVGRINEVSAYFEDTLRELLSSTSGLSCDSPRTIQGKVQHSGYPDLRII
                     DLASRRVFYLDPKLYAAGSRNSSFRTFYFEPKKATNKVRDDAVHFIVGFEHQPPQHSG
                     LTGQPSPRYDLAGERREKNGHWKFTRWDLVDLSHFKVKLKAEFQGSNRDMYRAEAIVA
                     TGSVGRDSVEPTK"
     gene            5376..6374
                     /locus_tag="VE486_06475"
     CDS             5376..6374
                     /locus_tag="VE486_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HZA38769.1"
                     /translation="MGTGLRKRPKEKMLHGQVGQLMMDKSEKIFVAGYRGLVGSALIR
                     HLEREGFQNLPKRDRSQLDLADERAVQNFFAEEKPVVVIFAAAKVGGIKANNDFPVEF
                     LLDNLRVQNNVIHAAHEAGVRKLLFLGSSCIYPKFAPQPIPETALLSGPLEPTNEAYA
                     IAKIAGVKLCQALSREYGANFISAMPTNLYGPNDNFDLETSHALAALLRKAHEAKTTG
                     AREFVVWGSGKPRREFLHVDDLASACLFLLEKYDSPEIINAGCGKDISIRELAELICD
                     VVGFDGELAWDTTKPDGTPRKLLDTTKLRKLGWQPTIPLRDGIAQTYDWFLKNVAR"
     gene            6460..>6719
                     /locus_tag="VE486_06480"
     CDS             6460..>6719
                     /locus_tag="VE486_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZA38770.1"
                     /translation="MKTLRVGVVGVGHIGSNHARLYAEIPSAEFAAVYDIDVVRGNAI
                     GRKFGATPTKSLDEFIELIDAASVATPTNTHYEIARSLLMRG"
BASE COUNT         1477 a         1911 c         1825 g         1506 t
ORIGIN      
        1 aagctcacgg agaaaatgcg ggatgggtgt ctgccgcttc ttttcgaaat agagatccca
       61 aacgtaacga ttcaccaagg cgacgacgag cgccgtgctc aacaagatca gcgccgcgcc
      121 aacgtgattt ggccacgacg catgcacgcc ataaacggcg atggccgcat aaaacgccag
      181 cgtcaggcag aagaggcgaa atagcagccc aagccgcact cctgcgcgtc gttttaagaa
      241 ccggccgatc ccgagcgcga taaaaaacgt gccgatgaac acgatcgccg tggctgcgcc
      301 acgctccgct ccggataatg cgttaaacat caggcgccga gtatatcaga gagcaactcc
      361 tcgaaacaag ttctctcgcg gatgcagatc ctgtagggcg ggcgcttcgc ctgccgacag
      421 cagcagtaac ggacgcgggt gcctacaatt ctattttgtt ggtgcgataa ccagccacgc
      481 ccagccggcc aggaaacaga gaccacctag cggtgtgacc gcgccgagcc agtccggcgg
      541 ctgccggatc agcgccatca cgtataaact cccgctaaaa agaaaaatgc cggcaaaaag
      601 gagccaccat gcgccgcgat tcgccgcgcc aaaaagcgca agcacgaaca tcgcgatcgc
      661 atgaatgaaa tgataaagca ctgccttatt ccagacatcg agcatgccat gagtctcgag
      721 cgttgatttg aggctgtgtg ccccaaaggc ccccagcgct acggcgagga aacagagcgc
      781 agctgcaatt cgaaaaaaca aaatcgacat cgtgatggtt acactttaac ggctcacctt
      841 acagcagatt gtcgaggtat acctggcgat caaacggggc gaacgcttcg agttgctctc
      901 ctgtgccgac aaagcgcgtt ggaattccaa gctcatcctg gattgcaacg acgatgccgc
      961 cctttccgct gccgtcgagc ttggtgacaa tgagaccggt gagcccgatt gcgctctgga
     1021 attcgcgagc ctggctgagc ccattgccgc cggtagtcgc atccactacc agcaaagcct
     1081 catgcggcgc actcgggtcc tgttttccga gagtgcgttt caccttttgc agctcggcca
     1141 tcaggttggt ttttgtgtgc atccggccgg ccgtgtcgca caaaagaaat tgaacgccgc
     1201 gtttggtcgc cgcctggtac gcctcataac aaagcgcagc cggatcgcta ttatattggc
     1261 cacggaccat ttcaacgccg aggcgttctg cccagatgcc tagctgctcg atggctgcgg
     1321 cgcgaaaagt gtcggccgcc gccaggagca cggcatatcg atccgttcga ctggcgctcc
     1381 ggacaggttg ttgttgcaga taacgcgcca gtttggctgt cgatgttgtc tttccagtgc
     1441 cgtttactcc caggagcaaa atcaccttcg gctgtccggg cagtggctga atcggcgccg
     1501 gatttgctgg taaaatgcgt gcgatttcct cgcgcactac tttcacaacg tcatttatgc
     1561 ccatgaccga ccaggcttca cggtcctgga gagtcttgat aattcgcatg gtcattggca
     1621 caccgagatc cgccctgatt agcgattctt ccagctcgtc ccagtcgatt ggcttgccgg
     1681 caaaatgttg ggcgagggat tgaagaaaat tcacgttgtc gtcgaagcgt tacaccgttg
     1741 accccttcat cgctcgtaat gtcatgttga gcgaaccgaa acatctctgg ttatcatttc
     1801 cgcgctatcg ggagaaacaa tcagagattc ttcgcgtcgc tcagaatgac aatgatgcct
     1861 gacgctttcg cttcttcgct tcacgaatca cgagcctttt ttcaccgcct cgcgcaacgg
     1921 gcgtttcaaa tcgtctttca cccgatcaag aataccgttc acaaatcggc cagattccgc
     1981 gccgccgaaa gcttttgcca gctcgatcgc ttcattgatt gagacaaccg gcggaatgtc
     2041 atcgcgataa agcatctcgt agatggcaag ccggaggata ttgcgatcga ctgccgagat
     2101 acgacggaat tcataattct cgcagtagcg ctgaatgcgt tcatcgattt ccggcagatg
     2161 cgcgaccatc ccctcgataa gaggctgcgc aaaatcacgg acacgcggcg tagccgggca
     2221 aaattcccaa aagtcctcga tatgctcggg cccctcgcca tgctggagat ctcgccagaa
     2281 atgatattgg atcgctgctt cgcgcgctcg tcgccgtttg cccataacac ttattctccg
     2341 cgaagctcag acatgacgcg ggcaatttct acagctgctc gcgcggcccc ggttccgcgg
     2401 ttgattttat tttcgaggca gcgctcgcac gcctgggtct cgtcatcaaa agaaagcacg
     2461 gcattgatca ccggcacggc gtgatcgact gcgattcttt gaagggcgtc tgtgacggaa
     2521 cggctcagat tttgtgcgtg gtcggtcttt cccttcaaga ttactccgca ggcaatgatt
     2581 gcgtcggttt ttttctgcga cgcaagttcg cgcacaacga cgggaatctc gaatgccccg
     2641 ggaactcgct ggatggagat cgtcgcgttc ggcgcgaaag cgcgcaattc tttttcggca
     2701 tgatcgacca aaccctgcac gtagcgcgca ttaaattggc tcgcgacgat ggagaacgtg
     2761 cgttttccct caacgacgcg ggggcgaggc ggatcggatt tggacattta acaatgacga
     2821 atgacgaatg acccaccttc gccaagcttc agcgtggcag gcgaatgacg agataatgat
     2881 gaagcaagaa tgacgaatga tgcattgcgc agcatttcgg acttcgtcat ttgccaatgt
     2941 cttacagcag gtgccccatt ttcgatcttt tggtctcgag atatttgacg ttgtgtgggt
     3001 tggcttcgct gcgaatcggc acttgttcca ccatctccag gccgtaaccc tcgagtccga
     3061 caactttctt cggattgttg gtcagaaaac ggaaccggcg gactcccaga tcgaacagga
     3121 tttgcgcgcc gaggccgtaa tcgcgcaaat cactcggata cccaagcctg gcatttgcct
     3181 ccaccgtgtc gaggccttcc tcctgcaatt tataggcgtg aatttttgcc gcgagcccga
     3241 tgccgcgccc ttcctgccgc atgtaaacca gaacgccgct gccttcctcc tggatttgtt
     3301 gaagcgccgc atgcagttga tttccacagt cgcatcggcg cgatccgaaa acgtctccgg
     3361 taagacattg gctgtgcacg cgcaccaggg ttggtttgct tttgtcgata ctgcctttta
     3421 ccagcgcgag atgatgctgg ccgtcgagct tcgagcggta cagatgtaaa tcgaaatcgc
     3481 cgtaatccgt cggcattttt acgatctgtt ccggttccac cagcttttcg cggacgcggc
     3541 gatgggcgat taaactttgg atggtgcaaa tgcgcaggcc atgccttctg cggaactcca
     3601 ttagctccgg cagtcgcgcc attgtgccat cgtcgtgcag aatctcgcaa agcactccag
     3661 ccggttgcag cccggccatg cgggcaagat cgaccgccgc ctcggtatgc cccgcgcgcc
     3721 gcagcacgcc gccgtccttc gcgcgcaatg gaaaaacgtg acctggttgg gcaaggtctt
     3781 caggcgatga ttttgaattg gcgatcgcca gaatcgtcgt cgctcggtcg tatgcgctga
     3841 tgccggtcgt cactccgcgc gccgcgtcca ccgacaccgt aaagtcggtg cgatacattt
     3901 cacgattctg cgccaccatc cgttgcagac cgagttgttc cgcgcgctcg ttcgagacag
     3961 gcgcgcaaat taaaccgcgg ccatgcgtag ccatgaaatt gaccgcctcc ggcgtcacct
     4021 tttctgcggc catcaccaga tcgccttcgt tctcgcggtc ggcatcgtcc gtcacgataa
     4081 cgatgcggcc cttggcgata tcgcttacca catcgtcgat cgtgtcgaac tgaaacgtat
     4141 cctttgtctt tgtaatcatc ggaataataa gactaccgtc gctgccgctc tcggcaaacg
     4201 taaatccgat tgccggtagg cgagactctg tcgagcccac gaatcaattc accagcgttc
     4261 attcatgatg ccctgatatg gccattcgct tgcatgcgtg attaaacctg cgcgcgcgca
     4321 actttacttc gtcggctcga cagagtctcg ccctaccgag cctgtcgcca cgattgcctc
     4381 cgcgcgatac atgtcgcgat tgctgccttg gaactcggct ttcaacttca ctttgaaatg
     4441 cgaaaggtcg accagatccc agcgcgtgaa tttccagtgt ccatttttct cgcgccgctc
     4501 gcctgccagg tcgtagcgtg gcgaaggctg gcctgtcaag ccggagtgtt gtggaggctg
     4561 gtgttcaaac ccgacgatga aatgcaccgc gtcatcccgc actttgttgg tcgctttttt
     4621 cggttcgaaa taaaatgtgc ggaagctgct gttgcggctg ccagccgcat aaagcttcgg
     4681 gtcgagatag aagacgcgtc tgctcgcaag atcgatgatc cgcaaatcgg gatagccgga
     4741 gtgttgtact tttccctgga ttgtgcgcgg ggaatcgcag ctcaatccag aggttgagct
     4801 gagcaactca cgcaaggtat cttcgaagta agcgctcact tcgttgatgc gcccgacatt
     4861 ctggatcgcg ctatctggcg cgttcaatcg cttcatcgtc tcgtcacacg ccgcgctgat
     4921 ttgtttgatt acacgctggt cgatttcatt ctttggatcg acggcgagaa ctctcttgcc
     4981 ggtcgcatcg aagatcactt ctttgaaggc aatcccgcgc aattgtcgat cttcatcaaa
     5041 cagccacggg attagttgat ccaccggtgc ctttgtagcc ggggtcgctg accccggcct
     5101 ggcttcgctt tcatcggtaa ccccgaaagg gttcggggtt gccctgcaaa tcgcaagcgc
     5161 gataaaaagg aggaacgcca ccagccatcg catgtgacga ttcaaaatcc atgctcgcga
     5221 ttgaacaaga gcaaatgaac gtggatctcg cgtcgcaatc cgaagaggct caaggagcgg
     5281 cggtctctag tccgccgtct ttcctcggta gtgtggagac tgaagttccc cgggatcgcg
     5341 acaaagcagg gtgttagtga gattcacggg actcggtggg aactggattg cgcaaacgcc
     5401 caaaagagaa aatgctacac gggcaggttg gacaattgat gatggataag tcggaaaaaa
     5461 tctttgtcgc cggttaccgc ggtcttgtcg gcagcgcttt gatccgtcat ctcgaacgcg
     5521 aggggttcca gaatctgccg aaacgcgacc ggtcgcaact tgatttagcc gatgagcggg
     5581 cggtgcaaaa tttcttcgcg gaagaaaagc cagtagtggt gattttcgct gcggcaaagg
     5641 tcggcggcat caaggcgaac aacgattttc cggtcgagtt tctgctcgat aatttgcggg
     5701 ttcagaacaa cgtcattcac gcggcccacg aagccggcgt gcgcaagctt ctctttcttg
     5761 gaagctcctg tatttatccc aagttcgcgc cacaaccgat tccggaaaca gccttactga
     5821 gcggcccgct cgagccaacc aacgaggctt acgcaatcgc gaagattgcc ggcgtcaaac
     5881 tttgccaggc gctttctcgc gaatacggcg cgaacttcat ctcggccatg ccgacaaatc
     5941 tttacggtcc gaacgacaac ttcgatctgg agacatcgca cgccctggcc gcgcttttgc
     6001 gtaaagcgca tgaagccaaa acaaccggcg cgcgtgaatt tgttgtgtgg ggatcaggta
     6061 aaccgcgccg cgaatttttg catgtggacg atcttgcttc ggcgtgtctt tttctgctgg
     6121 agaaatatga ttctccagaa atcattaatg ccggatgcgg caaagacatc tcgatccgcg
     6181 aactggcgga gttgatctgt gacgtcgtcg gctttgatgg cgaactggcc tgggacacga
     6241 ctaagccgga cggtacgccg cgaaaacttc tcgataccac aaaactgcgt aagcttggct
     6301 ggcagccgac gatcccgctt cgcgacggta tcgcccaaac ttacgattgg ttcctgaaaa
     6361 atgtggcgag atgagcgagg atcgacgcat gaacgaacac gcacccatag ggggcgccgt
     6421 tcgcgtcccg cgggaaacca ttagcttacc ttacagacca tgaaaacact gcgcgttgga
     6481 gttgttggcg ttggccacat cggaagcaat catgcgcggc tctacgcaga aattccgtcg
     6541 gccgagtttg ctgcggtgta tgatattgat gttgtaagag gcaacgccat cggacgaaaa
     6601 ttcggcgcca cgccaacaaa atcgctcgac gagtttattg aattaattga cgccgcgtcg
     6661 gtcgcgacgc cgaccaacac gcactacgag attgcgcggt ctctgctcat gcgcggcaa
//
LOCUS       DASQUQ010000228         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_52579, whole genome shotgun sequence.
ACCESSION   DASQUQ010000228 DASQUQ010000000
VERSION     DASQUQ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52579"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VE486_06485"
     CDS             <1..740
                     /locus_tag="VE486_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZA38771.1"
                     /translation="LAAGGLLFAFDHATIAGKLVLSLLAVASIFSWSIMITKLRVIRF
                     ARQQNARFLAAFRQDRQPLRLFEKNARFPGSPVSNVYRAGCEEMTFHLLGSPEVDDTF
                     RARLGIADKISPAQMGAVNAAMERAVGETALALESQMILLATAVSGSPFLGLLGTVWG
                     VMDAFTGVAVAGSPNLESMAPGVSGALITTVTALCVAIPAMFGYNFLVTSIRGMIVEM
                     DNFAAELASEFEHKYVDHSSREGAFRK"
     gene            853..1275
                     /locus_tag="VE486_06490"
     CDS             853..1275
                     /locus_tag="VE486_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZA38772.1"
                     /translation="MRRYSQRTGLTTLSEINITPLLDLAFVLLIIFMITTPLLENSMN
                     LSIPSSGATNPPINSSQVQTVSIDHDETIRFNNQVVNLETLTTQLIQLKQANPDVAIV
                     IRPDRDLPVQKLIGLMDAVQRAGISKVGIATRATTNGH"
     gene            1432..2349
                     /locus_tag="VE486_06495"
     CDS             1432..2349
                     /locus_tag="VE486_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HZA38773.1"
                     /translation="MGRNTRFWRNVTLIALAHVAVILGLIRWSMAARSSPNPQSIVWL
                     SSAQDLPAEESQPGVSPSAGVSISPPELEQPPKTDEVAEETPLVTAAKSEIELPAPTP
                     TPTSTPKPTATPRPEPKPTPKPTPKATPKKAVLAKASPKPSPKAKPSRTKSAEKTKKA
                     DTKAVGKKIAKTAPAKDASSSQSSSGETDLAGKDGHAGGGGSESEFGWYGNMLHDRFY
                     SAWIQPTTNIPSGAKISTLVKVRIERDGRVSKFEIIKPSENVVVNESVATIAKRVTHV
                     DPPPAGLGNGDHYDVKINFELNTDQEAGD"
     gene            complement(2538..>2775)
                     /locus_tag="VE486_06500"
     CDS             complement(2538..>2775)
                     /locus_tag="VE486_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38774.1"
                     /translation="ISRGGDTDYLVLQHTSGDVLLPPFTGPGLLLRFETPDGQPTILA
                     DCLTRPTSMTLDRRRSTLYISEFGGRVVTIPFTP"
BASE COUNT          683 a          764 c          757 g          571 t
ORIGIN      
        1 tgctagcggc cggcggtctc ctttttgcgt tcgatcacgc gacgatcgca ggaaagctcg
       61 tcctctctct actcgccgtc gcttcaatct tcagttggtc gatcatgatc acgaagttgc
      121 gcgtcatccg cttcgcccgg caacaaaacg cccggttcct cgcggctttt cggcaagacc
      181 ggcaaccgct gcgtttgttt gaaaagaacg cacgattccc ggggtcgccg gtatctaatg
      241 tatatcgcgc cggatgcgag gaaatgacat ttcatcttct cggttcgccg gaagtggacg
      301 acacctttcg cgcgcgactt gggatcgccg ataagatttc gccggcgcaa atgggcgcgg
      361 tcaatgcggc catggaacga gcggtgggcg aaacggcgct ggcgctcgaa tcgcaaatga
      421 ttttgctggc caccgcggtg agcggttcgc cgtttctcgg attgctcggc acggtttggg
      481 gggtgatgga cgcttttact ggcgtagcag ttgcaggatc acccaatcta gagtcgatgg
      541 cgccgggcgt gtcgggtgca ctgattacta ccgtcaccgc gctctgtgtc gccattcccg
      601 cgatgttcgg ttacaacttt ctcgtcacca gcattcgcgg catgatcgtg gaaatggata
      661 actttgccgc cgagctcgcc tccgagtttg agcacaaata cgtcgatcac agctcgcggg
      721 aaggagcctt ccgcaaatga gatcgatgaa ctggttcaat gttgagagtt cgatgttcgg
      781 tgttcgatgt ttccgatcga aagagaacgc cgaacatcca acatccaacg cccaacatcg
      841 aagcaggaac aagtgagacg ctattcacaa cgcaccggcc tgaccacgct gagcgagatt
      901 aatatcacgc cgctgctcga tctcgctttc gtgctgctca ttattttcat gatcacgacg
      961 ccgctgctcg aaaacagcat gaacctgtcg atcccatcca gcggcgccac taatccgccg
     1021 atcaattcgt cacaagtgca aacggtctcg atcgaccacg acgagaccat ccggttcaac
     1081 aatcaagtgg tgaacctgga aacgctcacg acgcaactca ttcagttgaa gcaggccaat
     1141 ccagatgtgg cgatcgtgat tcgccccgat cgcgatctgc ctgtacaaaa attaattgga
     1201 ctgatggatg cggtgcaacg agcgggaatc agcaaggtcg gcatcgccac acgagcaacc
     1261 acgaatggac actaatgaac acgaattcac aaagaggttg cccgctaatc acgcgaatcc
     1321 acgcgaagga atcggctttg ttattctttt attcgcggtc attcgcgtca ttcgcggaca
     1381 aatcttctgc gccttggcgt ccattggtgt gcattcgccc ttagaatagc gatggggaga
     1441 aatacgcggt tttggcgaaa cgtcacgctg atcgcgctcg cgcacgttgc agttatcctg
     1501 ggactaattc gctggagcat ggcagcgagg agttcaccaa acccgcaaag catcgtttgg
     1561 ttaagcagcg cgcaagatct cccagctgaa gagtcgcaac ccggcgtttc gccgtcggcg
     1621 ggggtctcaa tttctccgcc cgaactggag cagccgccga aaacggatga agtggcagag
     1681 gaaacacctc tcgtgaccgc cgcaaagagt gaaatcgaat tgcctgctcc gacgccgacg
     1741 ccaacatcta ctccaaagcc aacggcgact cccaggccag aaccgaagcc aacaccaaag
     1801 ccaactccga aagcgacccc gaaaaaagcc gtgctggcga aagcttctcc gaagccatcc
     1861 ccgaaagcga agccgagtcg gacgaaatca gctgagaaga caaagaaagc cgatacaaag
     1921 gcagtgggaa aaaagatcgc gaaaaccgct cccgcgaaag acgcatcctc atctcagagc
     1981 agcagtgggg aaacagattt agccggcaaa gatggacacg cgggtggcgg gggcagcgaa
     2041 tccgaattcg gctggtatgg caacatgcta cacgatcgtt tctacagcgc ctggatccag
     2101 ccaaccacga acatcccatc cggcgccaag atctcgaccc tggtcaaagt tcgcatcgaa
     2161 agggatggga gggtttccaa attcgaaatc atcaagccgt ccgagaatgt ggtggtgaat
     2221 gaatcggtag caacgattgc caaacgagtg acacatgttg acccgccgcc ggccggcctc
     2281 ggcaacggcg accattacga tgtaaaaatc aatttcgagt taaataccga tcaggaagcg
     2341 ggagattagg agggttctac gcgctgcaat aaaaaaaagt cccgctcgcg aaattagttc
     2401 ccaattaggt ttcttcgtta cggattgatc agcaaagtag gaaaattttt ggttttaacg
     2461 cgacaccctt gttctgatta tccgtggcat tcgtgctatt cgtggtcagc tggccagtct
     2521 ttacggattg ttcccaatca cggggtaaaa gggatcgtca caacgcgacc gccaaattcg
     2581 ctgatgtaaa gcgtgctcct tctcctgtcg agggtcatgg aagttgggcg cgtcagacaa
     2641 tcggctagga tagtgggctg accatccgga gtttcaaaac gcaacaagag gccggggccg
     2701 gtaaatggcg gcaagagaac gtctccggag gtgtgttgga gcaccagata atccgtgtcg
     2761 ccacccctgc tgatc
//
LOCUS       DASQUQ010000229         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_150970, whole genome shotgun sequence.
ACCESSION   DASQUQ010000229 DASQUQ010000000
VERSION     DASQUQ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150970"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VE486_06505"
     CDS             complement(<1..492)
                     /locus_tag="VE486_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HZA38775.1"
                     /translation="MTKLIDGRAIAENVYVDLRREIAELKSKGITPGLAGVLVGDNPA
                     SRTYVRSKDKMCRALGLHSVRLELPVSTTQAELLARVEELNRDPSIHGILVQSPAPKQ
                     IDEEAIVRALDPGKDVDGFHPENVAKLVIGDPSGFVPCTPLGVQRLLLESKIDIDGAH
                     VVIV"
     gene            complement(511..1104)
                     /locus_tag="VE486_06510"
     CDS             complement(511..1104)
                     /locus_tag="VE486_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZA38776.1"
                     /translation="MLQVWARTLRYQIDDRAALIGNPVRENYIGSLWHNRLLLISYVL
                     KRFAPHRPGAGLISASRDGDLVADLTQRFGFDVVRGSSSRMGASAILEMSNVLASGRD
                     LLITPDGPRGPVYELGPGIIFLAQKSGAPVLPIHLEYSSCWRLKSWDRFILPRPFSKV
                     RVIFGSLHRVGSTNTPEEFETERLRLQNAMMALVERR"
     gene            complement(1152..1724)
                     /locus_tag="VE486_06515"
     CDS             complement(1152..1724)
                     /locus_tag="VE486_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZA38777.1"
                     /translation="MKLVIAATGASGTIYLQRLLEQIDCAAHEIHLVMSSHAQLVAKQ
                     EVDHFKLPTDIAHHSENDMNVPFVSGSARFDAMVIVPCSMATIGRIASGSSDSALLRA
                     ADVFLKERRKLILVPRETPWNLIHARNVVTLLEAGAVILPAIPSFYSRPGSVTAVVDT
                     VVWRILDQIGLPNPSAYRWADNTAKPSRKH"
     gene            1925..2308
                     /locus_tag="VE486_06520"
     CDS             1925..2308
                     /locus_tag="VE486_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZA38778.1"
                     /translation="MVKKLLHTRFRVTDLEKTISFYKDVLGLEEVRRHTSGRGSQLVF
                     LKAPDSDEEIEICKFDESGPVVVGPDLTHLAFEVEDLDAFAKKAAAKGYPLSDGPHST
                     GTGRIAFIDAPEGYEIELIERGAKS"
     gene            2375..3391
                     /locus_tag="VE486_06525"
     CDS             2375..3391
                     /locus_tag="VE486_06525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZA38779.1"
                     /translation="MKLWRDRVLLLCALISINALSVSGQESDVEAVRPSGISGPVRKA
                     EPVEKEKSVRKAQAVEKEEEAKPTPARKRRAHAKAKRKVKSTPEQIEYTPDGIPKTAA
                     ASVIVVDANTGQTLYEKNADQTRAPASTQKLLTALIIAESGFLDRPVTVQPVDTLAEP
                     VKLNIKAGDTYQRIDLLRALLVKSPNDVARCLARDNAGSVEAFAQVMNRRARELGAVN
                     SHFVNPNGLPMPGQHSTARDLSLIARAAYANPTIRSIICLPQLVFRYANGRTRELENT
                     NKVLKRLPYCNGMKTGYTEAAGHCLISSGSRGGRDIIVVVLGDSKARVWRDSSALLSW
                     GLWL"
     gene            3472..>4256
                     /locus_tag="VE486_06530"
     CDS             3472..>4256
                     /locus_tag="VE486_06530"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HZA38780.1"
                     /translation="MSDFFQEQLQALRARSLDRHLREISSAQGAEIEITGRRLVNFSS
                     NDYLGLASDPRLREAAIAAINEFGVGAGASRLISGTQSPHLRLERALSKWKGTEAALC
                     FNSGYAAALGTIPALVTKNDVIVLDKLCHASLIDGAKLSGAILRVFPHNHLGKLESHL
                     QWAKREHAGRRILIITESVFSMDGDRAPLRELVELKKRFGALLLLDEAHAIGVIDQNG
                     RGLAGEENATEDVDVQMGTLSKALGASGGYICGSRNLIEWLINR"
BASE COUNT         1012 a         1127 c         1204 g          913 t
ORIGIN      
        1 aacaatcacg acgtgcgcgc cgtcgatgtc gatcttgctt tcaagcagca ggcgctggac
       61 cccgagtggg gtgcatggaa caaatccgct gggatcgccg attacgagct tcgccacatt
      121 ctccgggtgg aaaccatcga catctttgcc tgggtcgagt gcgcggacaa ttgcctcttc
      181 gtctatctgt tttggcgccg gcgattgcac aagaataccg tgaatacttg ggtcacgatt
      241 taattcctca acgcgagcaa gcagttcagc ctgggtggtg gataccggca gctcgagcct
      301 taccgaatgc aaaccgagag cccgacacat cttgtccttt gaacgcacat aggtccgcga
      361 ggccggatta tcgccaacaa gaacgccagc aagacccggc gtgatgcctt tcgatttcaa
      421 ttccgcgatt tcgcggcgaa gatcgacgta cacattttcc gcgattgcgc gtccatctat
      481 caacttcgtc atgcgccgaa cctagatgac tcagcgtcgt tcaaccagcg ccatcatggc
      541 gttttgtaaa cgcagacgct cagtttcgaa ttcctcaggc gtattggtcg atcccactcg
      601 gtgcagcgat ccgaaaatca cgcgcacttt cgagaacgga cgcggcaaga taaagcgatc
      661 ccagctctta agccgccagc agctcgaata ctccagatga atcggcagga ctggcgcgcc
      721 cgatttttgg gcgagaaaaa ttatgcctgg gccaagttca taaaccggcc ctcgcggccc
      781 atccggcgta atcaaaagat cgcgaccaga ggcaagcaca ttgctcattt ccaaaatcgc
      841 gctcgctccc attcgtgagc tcgaaccccg gacaacgtcg aatccgaaac gttgcgtcaa
      901 gtccgccacc agatcgccgt cccggctggc actgatcaag ccggcgccgg gtcgatgcgg
      961 cgcaaatcgt ttcaagacgt acgaaattaa aaggagacga ttgtgccaga gagacccgat
     1021 gtaattctcc ctaacgggat tgccgatgag ggctgcgcgg tcgtcgattt ggtaacgcag
     1081 cgtgcgcgcc cagacttgca acaaacgaaa cccaaatgcg attagccatc gcgcggctcg
     1141 accctcgatc ttcaatgttt gcgggaaggt tttgccgtgt tatcggccca gcgataggcg
     1201 ctcgggttgg gcaggccgat ttgatcgaga atccgccaca caaccgtatc tacgacagcc
     1261 gtaacggacc ctggccgact gtaaaaagag ggaatagctg gcaatatgac ggcgcccgcc
     1321 tcgagcaagg tcacgacgtt gcgcgcatga atgagattcc acggcgtctc acgagggacg
     1381 agaattagct tgcggcgttc cttcaaaaag acatcggccg cgcgcagcaa agcgctgtcg
     1441 ctggagccag aagcgatccg gcctatcgtc gccatcgagc aggggacaat gaccatggcg
     1501 tcgaaacgcg ccgaaccgct aacaaaaggc acattcatgt cgttttcgga atggtgcgcg
     1561 atgtccgtgg gaagtttgaa gtgatcaact tcctgctttg ctaccagttg agcgtggctg
     1621 ctcatgacca gatgaatctc atgcgcggca caatcaatct gctcgagtag cctttgcaga
     1681 tagatcgttc cgctagcgcc agtggcggca atgacaagtt tcatcgtgtg aaattattgc
     1741 tgagtttgaa tggcttcttt ttgaaaatgt tacccggtgt ttgtcattcc gagcgaagtc
     1801 gaggaatctc tcgttattaa acagtaaaag atgtctcgac ttcgctcgga atgacagggc
     1861 agagggctgg ttttcaatag gaggagtttg aatttaatcc ggtatgggag caataagata
     1921 cggtatggtc aaaaaactct tacacacccg tttccgggtg actgatctgg aaaagacgat
     1981 ctcattttat aaagatgtcc tcggtctcga agaggttcgc cgccacacgt ctggtcgcgg
     2041 ttcgcagctc gtgtttttga aagcgcccga tagcgacgag gaaattgaga tctgcaaatt
     2101 cgacgagagc gggccggttg tggttggacc tgacctcacg catctggctt ttgaggtgga
     2161 ggacctggat gcgtttgcga agaaggcagc ggcgaaaggt tatccgctct cggacgggcc
     2221 acattcgact ggcaccggca gaattgcgtt catcgacgcg ccggaaggct acgaaatcga
     2281 attgattgaa cgcggagcaa agtcgtaggt ccaaaattcc cgccttgctc tacggacttg
     2341 caatggtcaa aacctttccc ctaattttcc ggacatgaaa ttgtggcgcg accgggtttt
     2401 gctcctttgt gccttgattt cgatcaacgc gctgagcgtt tcgggccagg aaagcgatgt
     2461 cgaagcggtg cggcccagcg gtatctccgg gcctgtccga aaagcggagc cggttgagaa
     2521 agagaagtcg gtccggaaag cgcaggcggt agagaaagag gaggaggcga aaccaactcc
     2581 tgctcgcaaa cgccgggcac atgcaaaggc gaagcgaaaa gttaaatcga cgccagagca
     2641 gatagagtac acgccggatg gaataccaaa gaccgccgca gcttccgtga ttgtagtgga
     2701 cgcgaatacg gggcagacct tgtacgaaaa aaacgcggac cagacgcggg ctccggcgag
     2761 cacccaaaaa ttacttacgg ctctgataat agccgagagc ggctttttgg atcggcccgt
     2821 tacggtccag ccagtcgata cgctggccga gccagtgaag ctgaatataa aagcgggcga
     2881 cacttatcag cgcatcgatc tcctgcgagc gttgctggtc aaaagcccca acgatgtcgc
     2941 gcgatgtctg gcgcgggata atgccggcag tgtggaagcg tttgcccagg tgatgaacag
     3001 gcgtgcgcgc gaactcggcg cggtgaattc gcatttcgtt aatccaaacg gcttgccgat
     3061 gccgggtcaa cattcaacag cgcgcgatct ttcacttatc gcacgggcgg cgtatgcaaa
     3121 tccgacgatc cgatcgatca tttgtcttcc gcagcttgtt tttcgctatg ccaatggtcg
     3181 cacgcgggaa ctcgaaaata ctaacaaagt cctgaaacgt cttccttact gcaacgggat
     3241 gaagaccggt tacaccgagg ctgccggcca ctgcttgatc agcagcggat cgcgaggtgg
     3301 ccgcgacatc atcgtggtcg ttctcggtga cagcaaggcc cgagtgtggc gcgattcgtc
     3361 tgccctgctt tcctggggtc tttggctgtg agacacgaat ttcacgatcc acacgaattg
     3421 atctaaacgg attgcctcaa agtataagat tcgtgacaac ccgtttaatt cgtgtctgat
     3481 ttttttcagg agcaattgca agcgcttcga gcgcgttcgt tagatcgaca tcttcgagaa
     3541 atcagtagcg cgcagggggc cgaaattgaa atcaccggcc ggcggctggt caatttttcg
     3601 tcaaacgatt atctcggtct ggccagcgat cctcgattgc gggaggcggc aattgctgca
     3661 atcaacgaat ttggcgttgg cgccggagct tcgcggctga ttagtgggac gcaatcgccg
     3721 catttgcgtt tggaacgagc gctgtcgaaa tggaaaggaa cggaggcagc actttgtttc
     3781 aacagcggat acgccgctgc gctgggcacg atccccgcgc tcgtcacgaa aaatgacgtc
     3841 attgtgctcg ataaactctg ccacgcctca cttatcgacg gcgcaaaact aagcggcgcg
     3901 atcctgcgcg tatttccgca caatcatctg ggcaaactgg agagtcatct tcaatgggca
     3961 aagcgcgaac atgcaggcag gcggattctc attataaccg agtcagtctt ttccatggac
     4021 ggcgatcgcg cgccattgcg cgaattagtt gaactcaaaa agcgtttcgg cgcgttgtta
     4081 ctgctcgatg aagcgcacgc catcggagta atcgaccaga atggccgcgg cctcgctggg
     4141 gaagaaaacg caaccgaaga tgtcgatgtt cagatgggaa ccctgagcaa ggcgctcgga
     4201 gcaagcgggg gttacatctg cggttctcgc aatctcatcg aatggctgat taatcg
//
LOCUS       DASQUQ010000230         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_872354, whole genome shotgun sequence.
ACCESSION   DASQUQ010000230 DASQUQ010000000
VERSION     DASQUQ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872354"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            484..1179
                     /locus_tag="VE486_06535"
     CDS             484..1179
                     /locus_tag="VE486_06535"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HZA38781.1"
                     /translation="MKALGMIGGVGPESTIDYYQNIIGLYRERQRDGSYPQFIINSVD
                     LKKGLDFMAANDVAGMGDYLLEEIGKLARAGANFGLISANTPHIVFDDVASKSPIPLI
                     SIVEATCAAAKARKLKRLALFGTRYTMQATFYPRAFAREGIEIVVPDTKDQDYIHEKY
                     LNELVPGRFLPRTRAGLLAIVDRMKEKIDIDGVILAGTELPLILRDAEYKGIPFLDTT
                     KIHCQAAVAEMLS"
     gene            complement(1280..>2511)
                     /locus_tag="VE486_06540"
     CDS             complement(1280..>2511)
                     /locus_tag="VE486_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38782.1"
                     /translation="YAESQEGEVHRINLRNGELRRLRPEPHEGQPKYRFHWNSPLIGS
                     VHKPGVIYLAGNHVFRLTDRAEKYVVISPDLTRNDPARTVATGSGAENYGVIFSLAES
                     PKRAGLLWAGTDDGRLWITENDGGKWTELTDNLPEPARGQWVTRIEPSHLDANVAYVA
                     TSAYRAGDDHPIILRTADLGKTWQPVVGDLPPNAPVEVVREDLVNPKLLYAGTHFGVF
                     ASFDQGTHWIRIGDVPAVRVDDVQIHPRTSDLVIGTHGRSIAILDDTVPFRELTPEIA
                     AKPAHLFSVRSVTGAYLREGFADWNGKGVYRGANPPEGALFTVWVKEFTGDEIKIAVT
                     KATGQPVANLKAPGAPGFTRLNWDLRPTKDVSIEYGGDDPKRLLPAGDYTAELSFGAL
                     KTKQNFRVDLAEGITPR"
BASE COUNT          522 a          705 c          697 g          587 t
ORIGIN      
        1 tgtattgact cgtgctgaat gagaaagcgc aaacgaactt ttgagaatag ctagctgtag
       61 ccgggatcga cttgtcacgc cgtagccttg gcgaaggcgg atgatcccgg ttctttcatt
      121 cattttcttc caattcggca agcatttgtc gtttcaaatg ccgccgcgcc gatttcttga
      181 tggcgcgccg ttccgccgcg acagcttgct tttccgcctg tcgttcgcga cgcgtccgtg
      241 tgtctgcgga tctgggcaga tgtgcgatcc tcggacgttg cgcgccgact tttcgctctt
      301 ttggttttgc gtacggatcc attaagcggt tgagcgcttc ttgtttgcaa tgacgaagcc
      361 agaataaaaa taaggatgca cggcctgttc ggcgttcgtc atttaatcat tccttcgcca
      421 ttcgtcattc ggatttcgtc attggctaac tcgtgtttat tggaattcat tcgtcactga
      481 aacatgaaag cccttggaat gatcggcggc gtcgggccgg aaagcacgat cgattactat
      541 cagaacatta ttgggctcta tcgcgagcga cagcgtgatg ggagctatcc gcaatttatc
      601 attaacagcg ttgatctaaa gaagggactc gatttcatgg ccgccaacga tgttgcgggc
      661 atgggcgact acctgctcga ggaaattggc aaactcgcgc gggccggtgc aaacttcggc
      721 ctgatctcag caaacacgcc tcacatcgtg ttcgacgacg ttgcgtccaa gtcgcccatt
      781 ccgttgatca gtattgtcga agcaacgtgt gcggctgcca aagcgcggaa gttgaaacgg
      841 ctcgctttgt tcgggacgcg atacaccatg caggccactt tttatccaag agcctttgcc
      901 cgagaaggaa tcgagatcgt tgtgcccgat acgaaggatc aggattacat acacgagaaa
      961 tacctgaacg aattggtgcc gggaagattt ttgccgcgga cgcgcgccgg tcttctcgca
     1021 atcgtcgatc gcatgaaaga aaagatcgac atcgatggcg tcattctcgc cggaacggaa
     1081 ctccccctga ttctgcgcga tgcggagtac aagggaattc catttctgga cacgacaaaa
     1141 attcattgcc aggccgctgt cgcggagatg ttgtcgtagt atgaggcgca tcccccggcc
     1201 ggggtcaccg ctcccggcta gagctcgcgg atggccgggt cagcgaccgc cggctacaga
     1261 ttcgccgata gggtttctgt tagcgcggcg tgattccctc agcgagatcg acgcggaagt
     1321 tttgtttcgt ttttagagcg ccgaaactca attcagcggt gtaatcgccg gctggcagca
     1381 gccgcttcgg atcatcgcca ccatattcaa tgctcacgtc tttggtggga cgcaggtccc
     1441 aattcaatcg tgtgaaaccc ggcgcgcctg gcgccttcaa attggctacg ggttgcccgg
     1501 tcgctttggt gacagcgatt ttgatttcgt ccccggtaaa ttctttcacc caaacggtaa
     1561 acagcgcgcc ctcgggcgga ttcgcgccgc gataaacgcc tttgccgttc caatccgcaa
     1621 atccttctcg gagataagcg ccggttacgg aacgtacgct aaacaagtgc gcgggttttg
     1681 ctgcaatttc aggcgtgagc tcgcggaagg ggacggtgtc gtctaagatc gcaatgctcc
     1741 ggccgtgcgt cccaatcacc agatcggatg tgcgcggatg aatctgcacg tcgtcaacgc
     1801 gcaccgccgg aacatcgccg atacgaatcc agtgtgtccc ctgatcgaaa gacgcgaaca
     1861 ctccgaaatg cgtgccggcg taaaggagct tcggattcac aagatcttcg cgcacaactt
     1921 cgaccggcgc gttcggcgga agatcgccca ccaccggctg ccacgtcttg cccagatcgg
     1981 ccgtgcgcag aatgatcggg tgatcgtcgc cggcgcgata cgcgctggtc gccacgtaag
     2041 cgacgtttgc atctaaatga gaaggttcga tgcgtgtcac ccattgtccg cgcgccggct
     2101 ccggcaaatt atccgtcagc tccgtccatt tgccgccgtc attttctgtg atccaaaggc
     2161 ggccgtcatc ggttcccgcc cataataaac cggcgcgctt cggcgattcc gcgagtgaaa
     2221 agatgacgcc gtaattttcc gcgccacttc cagtggcaac tgtgcgggcg ggatcgttgc
     2281 gcgtgaggtc cgggctgatc accacgtatt tctccgcgcg atcggtcaac cggaaaacgt
     2341 ggttgccagc caaatagatt acgcccggtt tatgcacact gccgattaac ggcgaattcc
     2401 aatgaaaccg atatttcggc tggccctcat gcggctctgg gcgcaaccgg cgcagttcgc
     2461 cattgcgcaa attgatccga tgaacttcgc cttcctggct ctcagcgtaa a
//
LOCUS       DASQUQ010000231         9749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_527599, whole genome shotgun sequence.
ACCESSION   DASQUQ010000231 DASQUQ010000000
VERSION     DASQUQ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527599"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /gene="trxA"
                     /locus_tag="VE486_06545"
     CDS             complement(<1..239)
                     /gene="trxA"
                     /locus_tag="VE486_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZA38783.1"
                     /translation="MDNSATINLDESNFDREVTQSDKPVIVDFWAEWCGPCKMIAPLL
                     DEVAREKAGAVKIGKVNVDDNQSLSFKYNIRAIPA"
     gene            complement(244..405)
                     /locus_tag="VE486_06550"
     CDS             complement(244..405)
                     /locus_tag="VE486_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38784.1"
                     /translation="MKKTQPLIAMPHAASRGTSIAVAPEEKSAMPLCWALLGVSAVIL
                     IIQIWTYFS"
     gene            complement(952..2430)
                     /gene="rimO"
                     /locus_tag="VE486_06555"
     CDS             complement(952..2430)
                     /gene="rimO"
                     /locus_tag="VE486_06555"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZA38785.1"
                     /translation="MKIIAKAEDFARKPKIGMISLGCAKNLVDAEIMLGSALQRGMEI
                     TARAEDADVLVINTCAFIDSAKEESIDAILEAHQQRGLNRRPNQKLIVSGCMSQRFAK
                     ELREEMPEVDAFIGLDQVSQLGEIIEKILRKHPTSNIELPTSKAETSTFSVGRSMFDV
                     SSSEDHNSGPAFVTPRPTYIPDYDTPRFRLTPAHSAYVKIAEGCNHPCSFCVIPQMRG
                     RHRSRPIQSVIAEIQALVSEGVREINLISQDTTYYGMDLWPKKAGPRQPVDSARGPTL
                     AGLLREIQRIEGEFWVRLLYTHPAHWSDELIETIAECDKVARYIDIPLQHIDNTMLER
                     MRRETSRAHIENLIDKLRSGIPGVALRTTFIVGFPGETEGEFETLLNFIQKTRFERLG
                     IFKYSQEDGSRAAKMPNQIPLKLKNARYRMAMLIQQGIAHEIAGEKVGSELKLLVDQP
                     HIARSEGDAPDVDARVVLSKAAPVGEFVWREITGNRGYDLLA"
     gene            complement(2550..3515)
                     /locus_tag="VE486_06560"
     CDS             complement(2550..3515)
                     /locus_tag="VE486_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZA38786.1"
                     /translation="MTIEGLGKKFQEARRARDLTLDEAARMTKIRPARLAEIEADDFS
                     QFPSLAYAKGFLLIYGKFLDVDVTPYLDAFEDSERVTVDGYSYLQDNPPPKPVSAPVA
                     RRSSRASRPSRPSRDRISPMPLIFGVLALVIGFSVMKLILNLQRLAPGPAQPVAQTSP
                     VSASTPSLTTAPPVTAETPANNVASASATTPAGSPAPTIGEIPKAVGVSTARPAPGEP
                     EVRRAKPVLPGDLAKPQEGANSPATESSSPNRVAIRPLKRTYLRVIVDDQSEKPTFER
                     WVSPADGTVEFRGNHVSIRVLERDAVEIIKNGKPIEGDDEDVTVD"
     gene            complement(3530..5983)
                     /locus_tag="VE486_06565"
     CDS             complement(3530..5983)
                     /locus_tag="VE486_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZA38787.1"
                     /translation="MARTDKHSAWNEVFALVLLFVGTLLFFALISYTPKDVPSWIWFS
                     RSAPANHPAQNFIGPVGAIVAGICYQLMGAAAYLLAAILLGFGAAKLFHPLLRVTRRL
                     PWIVLFIWSGACLLHLQPYHLRGWHDAFNIQGPGGWIGYAFADERHGLLRSALGPFGS
                     LILLIGVYATTLILVTGLRPIHLVREMVAAIRRASIKLQEWRLQRRFRKADLKEKLEI
                     SERHLAKQRRLYEKELKKKGIAVPQSAAPVISPDELADRPKPKVVDTTALPTEQASAR
                     RKPSLAELRAGEEKTRPAPGLTSREWKSENYVLPGADLLDEHNLEGRAAADPSELEHV
                     QQILIDTLAQFGIAVAPGDITKGPTITRYEVYPAKGVRVDKIVSLERDLARATRAERI
                     NILAPIPGKDTVGIELANTRKVTVKLRELLQSPDWEEARGHAKIPLALGKDVYGKTII
                     ADLAQMPHLLVAGTTGSGKSVCINALVASMLFRFTPEELRFIMIDPKVVEMQFYNSLP
                     HLAFPIVTDPKKVLLALRWLIDEMERRYKIFARVGVRNIVGFNARPVKKTQKELDDEA
                     VESGASPDNARRSAEADDATIKVPREGELIIPDKIPYIVVIIDELADLMQTAPADVEN
                     AIARITQMARAAGIHLIVATQTPRADVVTGVIKANIPSRIAFQVASKIDSRVILDENG
                     ADRLLGQGDMLYLPPSTSRLIRAQGVLVTDDEIHRLVEFVSAQSPPAFDAAMHEKLQS
                     AAAPEEEVTEEDEELVEKCLEIIRQEKRASTSLLQRRLRLGYTRAARIVDILEQRGIL
                     GPGEGAKPREILVDLDAAV"
     gene            6397..7824
                     /locus_tag="VE486_06570"
     CDS             6397..7824
                     /locus_tag="VE486_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HZA38788.1"
                     /translation="MAKYKYATKPPNLSTMPPGVPYIIGNEAAERFSFYGMRSILIVF
                     MTTYLVNTTGELAPMGDAQARAWFATFVSAVYFLPILGAILAEGFLGKYNTIFSLSIV
                     YCLGHLALAFMDSAWCIAFGQEKVLAIGLGLIALGSGGIKPCVSANVGDQFGESNKHL
                     ISKVFGWFYFSINAGSFISTILCPLMLANPNWGPRWAFGIPGVAMFIATIFFWAGRKK
                     FVHIPAAGWGFVKQMFSKEGLGALGRLVIVYIFVAIFWSLWDQSSGGSWTLQARNMDL
                     NFLGMQLLPAQVQTANPILILLFIPLMNYVIYPLIDKVFPLTPLRKIGIGLFLTGFSF
                     VVIAFIQSMIDAGQRPTVWWQFLAYIILTLGETMVSITGLELSYTQAPNKMKSAVMAA
                     WLFCVSLGNAFTAGVNFFISNPDGSARMSDFNYYLFFSGLMLAASAIFAVVANFYRYR
                     TYLQSQEPTADELATEPALAGGAPT"
     gene            7832..9562
                     /locus_tag="VE486_06575"
     CDS             7832..9562
                     /locus_tag="VE486_06575"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZA38789.1"
                     /translation="MKAITITLALSLFFPLTRVRATPEDEGFQKLARDYIERELQANP
                     ENATELGDHRFDGQLTDYSPEARAKELAAQKAFRDKLNAIDGSGLTGANNIDFRILKE
                     NIHYKIFQAQELKEVDWNPLVYMQSLANSLYLLVARDFAPPEKRIPNLRQRMEKIPRV
                     IAQAKANLQHPPRVHTETAIEQTQGAVSLVREGLAPLLDQSPQLKKELAPLQEKTAAA
                     LEDYKKWLQNDLLPRSDGDFRLGPEKFRKKLRFALASDLSMEEIMKRARADLQQTQTA
                     IYETALPLYKKYFPKGDDKTLADKHKVTAAVLDKLAEQHPDDATVVGYAKRIVGEATD
                     FVKQHNLVTVPEVPLDVIAMPEFKRGVAIAYCDSPGPLEKNGKTFYAVAPTPKDWSKE
                     RRESFFREYNNYMIRDLTVHEAMPGHYLQIAHANEFKAPTLVRAIFSSGTFIEGWAVY
                     TEQMMAEQGYGGPEVKMQQLKMRLRAICNAMLDQSIHAGNMSEQEALDLMMKEGYQQE
                     GEAVAKWKRARLTSAQLSTYFVGATEHLDLRAAEEKKLGKDFDLKKYNDQVISYGSPP
                     VKYVRELMGL"
BASE COUNT         2088 a         2771 c         2697 g         2193 t
ORIGIN      
        1 aaagctggga tggcacgaat gttgtacttg aatgaaaggc tctgattgtc gtccacgttc
       61 actttcccaa tcttgaccgc gcccgctttc tcacgggcaa cctcatccag cagcggtgcg
      121 atcattttgc agggaccgca ccattccgcc caaaaatcga caatcactgg cttgtcgctt
      181 tgggtcactt cgcggtcaaa gttggattca tcgagattga tagtagctga attgtccatt
      241 gttttaagaa aagtaagtcc aaatctgaat aatcaaaatg acggcggaga caccgagaag
      301 cgcccagcac agcggcattg cacttttctc ttccggggct accgcaatcg acgtgccccg
      361 ggaggcagcg tgcggcatgg cgatgagggg ctgggtcttt ttcatctgga cggtagagac
      421 agacttggag ggcgagtctg gcatgcgcgc gacgcgcgcc gtctcttttt tcgggcccga
      481 tgaaagtgct tctggcagga tagcgactgg caggggcgcc gacggtgccg cgacgccctc
      541 gggcgaggtc agaacttgcg cggcgggttt tgaaacgagc ggcggcggct ctgttggagt
      601 gcgaggaggc aactcgtccg ccaacggacg gaatcgctgt ggcgagtctg acggctggcg
      661 agggccaagc tggactcgca cggtctcgcg cgattttgta acctggccgg gaggtttcga
      721 tccagactga ggtggcaggt caatgcgaac ggtctgttct tttggttcgt ttgagtcggc
      781 catcggggtg aaataagtac caccaaattt gcccaagaaa ccggctgctg tcacctgcta
      841 agtgaatttt caggatcaaa aactcgactg ctgccgcgct acagctgtgg tcgcctgtgg
      901 agcttcggat ctccccctgg gcccgccggc cacacagaga agatgtctgt gctacgccag
      961 gagatcatat ccgcggtttc ctgtgatctc gcgccagaca aattcaccca cgggcgctgc
     1021 tttggacaag acaacgcgcg catcgacatc tggggcgtcc ccttcgctcc gggcaatatg
     1081 cggctgatcg accagtaatt tgagttcgct tccaactttt tcgccggcaa tttcatgcgc
     1141 gatcccttgc tgtatcaaca tcgccatccg atagcgcgca tttttgagct tcagcgggat
     1201 ttggttcggc atcttcgctg cccgcgaacc gtcctcctgc gaatatttga agatgccgag
     1261 gcgttcaaat cgagtcttct gaatgaaatt gagcagcgtt tcgaattccc cttccgtctc
     1321 cccggggaat ccgacgatga aagtcgtccg caaggcaact ccaggaattc cggagcgcag
     1381 tttatcgatc aaattctcga tgtgtgcacg cgacgtttcg cgccgcatgc gctccagcat
     1441 cgtgttgtcg atgtgctgca acggaatatc gatgtagcgc gcgaccttgt cgcattccgc
     1501 gatggtctca atcaactcat cgctccaatg cgcggggtgg gtatagagca ggcgcaccca
     1561 aaattcgccc tcgatccttt gaatctcgcg aagtaaccca gcaagggtcg ggcctcgcgc
     1621 tgagtcaacc ggctgacgcg gccccgcctt ttttggccac agatccattc cataataagt
     1681 tgtgtcctgg ctgatcaaat tgatctcgcg cacgccttca ctgacgaggg cctggatttc
     1741 tgcgatgact gattggatgg gccggctgcg atgtcttcct cgcatttgcg gaattacgca
     1801 aaaactgcac gggtgattgc agccttcggc gatctttacg taagcggagt gcgccggcgt
     1861 aagcctgaag cgcggagtgt cgtaatctgg gatgtaagtt gggcgcggtg tgacgaatgc
     1921 aggaccgcta ttgtgatctt ccgaagaaga aacatcgaac atcgaacgcc caacactgaa
     1981 cgtcgaagtc tctgccttcg aggttggaag ttcgatgttc gatgttggat gttttcttaa
     2041 aatcttctcg atgatctcgc caagctgact gacctgatcg agtccaataa acgcgtccac
     2101 ctccggcatt tcctcgcgca gttctttggc aaatctttgc gacatgcagc cgcttacgat
     2161 cagtttttga ttcggccgcc tgtttaaacc gcgctgctga tgcgcctcca gaatcgcgtc
     2221 aatcgattct tccttggctg aatcgatgaa ggcacacgtg ttaattacca ggacatcggc
     2281 atcttctgcg cgcgctgtaa tttccatccc gcgttgcaga gcgctgccga gcatgatctc
     2341 cgcatcgaca agatttttgg cacagccgag gctgatcatg ccgatcttcg gtttacgagc
     2401 aaaatcctcc gccttcgcga ttatcttcat tctgcaggta taaaaacatc caacatcaaa
     2461 caccaaacat cgaatgtgga acgcctgctg acgatttatc cgagttcggc gttgggagtt
     2521 ggacgttgga tgttgggcgt ttttgtattc taatcgaccg ttacgtcttc gtcgtctcct
     2581 tcgatcggtt tgccattctt gatgatctca acggcatcac gctcgagaac gcgaatggag
     2641 acatggttgc cgcgaaattc cactgtgccg tcggctggag aaacccagcg ttcgaacgtc
     2701 ggcttttcgc tttgatcgtc cacgataacc ctgaggtagg ttcgtttaag cgggcgaata
     2761 gcgacgcgat ttggggaact tgattccgtc gccggtgaat tcgctccctc ttgcggcttc
     2821 gcgagatccc cgggaagcac aggcttggct cgacgcactt ccggctcgcc cggcgctgga
     2881 cgagcagtcg aaaccccgac cgctttcggg atttcgccga ttgtcggcgc gggcgaaccc
     2941 gctggcgtgg tcgcagaagc cgacgcaaca ttgtttgccg gcgtctcggc agtcaccggt
     3001 ggtgcagttg tgagacttgg ggtggacgcg gaaactggag acgtttgggc gacaggctgc
     3061 gctggtcccg gcgcaagtcg ctgcaaattg agaattaatt tcattaccga aaagccgatc
     3121 accaacgcga ggacgccaaa gatgagcggc atcggcgaaa tgcggtcgcg actggggcga
     3181 ctgggccgac tggcgcgact ggaacggcga gccactggcg cactgactgg cttaggcggt
     3241 gggttgtcct gcaggtacga atagccatcg accgtaaccc gctccgaatc ctcaaaggca
     3301 tcgagatacg gcgtaacatc tacatccaaa aactttccgt aaatgagcag gaaccccttc
     3361 gcgtacgcca gactgggaaa ttgagaaaaa tcgtccgcct caatctccgc cagccgggcc
     3421 ggacggattt tcgtcatccg cgctgcttcg tcgagggtaa gatcccttgc tcgccgcgct
     3481 tcctgaaatt tttttcccag accttcgatc gtcattgcgt caacgccggc taaacggcgg
     3541 catcgagatc gaccagaatc tcgcgtggtt tggcgccttc gccgggacct aaaataccgc
     3601 gctgctcgag aatatcaacg atgcgcgcgg cacgcgtgta gccgaggcgc aaccggcgct
     3661 gaagcaacga cgtggaagcg cgtttttcct gccgaatgat ttcgagacac ttctccacca
     3721 gctcttcgtc ttcctcggtc acttcttctt ctggtgcggc ggcggattga agcttctcat
     3781 gcattgctgc atcgaaagcg ggtgggcttt gcgcagaaac aaattcaact aggcgatgaa
     3841 tttcatcgtc ggtcacaagc acgccctggg cacgaatgag gcgcgaagtg ctcgggggca
     3901 gatagagcat gtcgccctgt ccaagcaaac gatcggcgcc attttcatca agaatcaccc
     3961 ggctatcgat cttggacgcg acctggaacg cgatgcggct gggaatattc gctttgatta
     4021 cgcccgtgac gacatccgcg cggggcgttt gggtggcgac aatgagatga atgccggccg
     4081 cgcgagccat ctgcgtgatt cgcgcaatcg cattctcgac gtcggcgggt gcggtctgca
     4141 ttagatcagc cagctcgtcg atgatgacca cgatgtaggg aattttgtcg ggaatgatca
     4201 attcaccttc gcgcggcact ttgattgtag cgtcatctgc ctcagcggag cggcgcgcgt
     4261 tgtccggtga ggcaccggac tctacggctt catcatccag ttccttctgc gtctttttca
     4321 ccgggcgggc attgaatccg acaatgttac gcacgccgac gcgggcaaaa attttatacc
     4381 ggcgttccat ctcatcaatc agccagcgca aagcgagcag cactttcttt ggatcggtca
     4441 caatcgggaa agcgagatgt ggcagagaat tataaaactg catctcaact actttcggat
     4501 cgatcatgat gaaacgcagc tcttctggcg taaaccggaa gagcatgctc gcgacgagcg
     4561 cgttgataca aacgctcttg ccgctcccgg tggtgcccgc caccagcaaa tgcggcattt
     4621 gtgccaaatc ggcgatgatc gttttgccgt aaacgtcttt cccgagcgcg agcggaattt
     4681 tggcgtgccc tctcgcctcc tcccagtccg gtgactgcaa gagttcgcgc agtttcaccg
     4741 tcaccttgcg cgtattcgca agctcgatgc ctacggtgtc ttttccggga atgggcgcga
     4801 gaatattaat tcgctcggcc cgcgtcgcgc gggcgaggtc ccgctccagg ctcacgatct
     4861 tgtccacgcg aacgcctttc gccggataca cttcgtagcg cgtaatggtc gggccttttg
     4921 tgatgtcgcc cggcgccact gcaatgccga actgtgccag cgtatcaatc aggatttgct
     4981 ggacgtgctc cagctccgat ggatctgccg cggcgcgccc ttccagatta tgttcgtcca
     5041 gtaaatccgc tccgggcagc acataatttt ccgatttcca ctctcgactc gtaaggcccg
     5101 gcgccggcct cgttttctcc tcgcccgcac gcaattcggc gagcgacggt tttcgacgag
     5161 ccgacgcctg ctcggtcgga agtgccgttg tatcgactac cttcggcttt ggccgatctg
     5221 cgagttcgtc cggcgaaata accggtgccg cagattgagg cacagctatg cctttctttt
     5281 tcaactcctt ttcgtatagc cgccgctgct tcgcgaggtg cctttcgctg atttccagtt
     5341 tctctttgag atcagctttt ctaaacctgc gctgcaatcg ccattcctgt agttttatgc
     5401 tggcgcgccg gatagcggct accatctcgc gaacaagatg aatcggacgc agtccagtca
     5461 ccagaatcag agtcgtcgca tagactccaa tcaagaggat aagcgaaccg aatgggccaa
     5521 gcgcgctgcg gagtaaaccg tggcgctcat cggcaaaggc gtatccgatc cagccgccgg
     5581 gtccctggat attaaaggca tcatgccagc cgcgtaaatg gtacggttgc agatgcagca
     5641 agcaggcgcc cgaccagata aaaagaacga tccacggcaa gcgccgggtc acgcgcagga
     5701 gtgggtgaaa tagtttggcg gctccaaatc cgagcagaat cgccgccaaa aggtacgctg
     5761 ccgcgcccat taactggtag caaatccccg cgacgatcgc gcccacaggc ccgatgaagt
     5821 tttgcgccgg gtggttcgcc ggcgcgctgc ggctaaacca aatccacgac ggcacatctt
     5881 tcggcgtgta ggagatcagc gcgaaaaaaa gtaatgtgcc gacgaaaagc agaacgagag
     5941 cgaagacttc gttccaagcg ctgtgtttgt ccgtgcgagc caccgcgtct gatagtaatc
     6001 ccgtggacgc ttctttcaat catttgtcgg gggcacaggc aacttgcctg tggggccagc
     6061 gggcttccag cgtgctggac tgtccaggcg agacctctgg ccgccccccg gcttgcttta
     6121 ggcttgcacg cggcgagcga cgcccattag tgtgccagcg cattatggct tttctgtttc
     6181 gccacaccgt cttcattgtt gcgatcggcc tgctgctgtt ttgtgcctct tgcgaaaaac
     6241 atccgctccc cgaagcgcga aacgtccaac cggaacacgt cgctccggaa aaacactcat
     6301 ccgagtcggg gaagatagag actgaaaaac ctttgccctc ggcaacccca gccgaatctg
     6361 tcccggcgaa tccgcatccc taaccgaaac ccgcccatgg ccaaatacaa atacgcgacg
     6421 aagccgccga acctcagcac catgccgccc ggtgttcctt acatcatcgg caatgaagcg
     6481 gccgagcgtt tctcgttcta cgggatgcgc tccatcctga tcgttttcat gaccacctac
     6541 ctcgtcaaca cgacggggga gctggcgccg atgggcgatg cacaagcgcg cgcgtggttc
     6601 gccaccttcg tctcggccgt ctatttcctg cctatcctcg gcgcgatcct ggcggagggt
     6661 tttctcggca aatataacac catcttctcg ctctcgatcg tttactgtct tggacatctc
     6721 gcgctggctt ttatggacag cgcgtggtgc atcgccttcg gccaggaaaa ggttctggcg
     6781 atcggtctcg gactgattgc gctgggttcc ggtggcatta agccgtgcgt ctcggctaac
     6841 gtcggcgatc agtttggcga atcgaacaag cacctgatct caaaggtctt cggctggttt
     6901 tatttctcga tcaacgccgg ctcgttcatc tcgaccattc tttgcccact catgctcgcg
     6961 aatccgaact ggggaccgcg ttgggcattc ggcattccag gcgtcgcgat gttcattgcc
     7021 acgattttct tctgggccgg tcgcaaaaaa tttgtgcaca tcccggctgc aggctggggc
     7081 ttcgtgaagc agatgttcag taaggaaggc ctcggtgcgc tcggccggct agtcatcgtt
     7141 tatatcttcg ttgcgatctt ttggtcgctc tgggaccaaa gctccggtgg atcatggaca
     7201 ctccaggcgc gcaatatgga cttgaacttc ctcgggatgc agttgctccc cgcgcaggtg
     7261 caaacggcga acccgatcct gatcctgctc ttcatcccgc ttatgaacta cgtcatctat
     7321 ccgctgatcg acaaggtttt tccgctcacc ccgctgcgga agatcgggat cggactcttc
     7381 ctcaccggct tcagcttcgt tgtaatcgct ttcatccaga gcatgatcga cgccggtcag
     7441 cggccgaccg tctggtggca attcctcgct tacatcatcc tgactttggg cgaaacaatg
     7501 gtgtcaatca ccggcctcga gctttcctac acgcaggcac cgaacaaaat gaagagcgca
     7561 gtgatggccg cctggctctt ctgcgtgtcg ttaggcaatg cctttaccgc cggggtaaat
     7621 tttttcattt cgaaccctga cggcagcgcg aggatgagcg acttcaacta ctatctcttt
     7681 ttctccggac tgatgctggc cgcgtccgcg atcttcgccg tcgtcgcgaa cttctatcgc
     7741 taccgcactt acctccagtc gcaggagccg accgcggatg agctcgccac ggagccggct
     7801 ctcgctggtg gcgcaccgac ttgatcataa aatgaaagcg atcacaatca ccctcgcgct
     7861 gtccctcttt ttcccgctga caagagttcg cgccacgccg gaggatgaag gatttcaaaa
     7921 gcttgcgcgt gattatatcg agcgggagtt acaggcaaac cctgagaatg cgaccgagct
     7981 cggtgatcat cggtttgacg gccaactaac cgattactcg ccagaggcgc gggcgaaaga
     8041 actcgcggca caaaaagcgt ttcgcgacaa gctaaacgcc atcgacggat ccgggctcac
     8101 cggcgccaac aacatcgatt ttcggattct caaggagaac atccattaca agatcttcca
     8161 ggcgcaagag ttgaaggagg tggactggaa tcctctggtt tacatgcaga gcctggccaa
     8221 cagcctttat cttttggttg cgcgcgattt cgctccgcct gaaaagcgga ttccaaattt
     8281 gcgccagcgc atggagaaaa ttccgcgcgt gatcgcacag gcgaaagcca acctgcaaca
     8341 tccgccgcgc gttcacacgg agacagcgat tgagcagaca caaggcgcag tcagtctcgt
     8401 gcgcgaaggc cttgcgccgc tgctcgatca gtctccacaa ctgaagaagg agcttgcacc
     8461 gttgcaggaa aaaactgcgg ccgctctgga ggattacaaa aaatggctcc agaacgatct
     8521 gttgccgcgc tccgacggcg atttccgtct cggcccggag aagttccgaa agaagctgcg
     8581 gttcgcgctt gcctcggatt tgtcgatgga agaaatcatg aaacgggcaa gagccgacct
     8641 gcaacaaacg caaactgcga tctacgagac cgcactgccc ctctacaaaa aatattttcc
     8701 gaagggcgat gacaaaacgc tcgcggataa acacaaggtc acggccgcgg tgctcgataa
     8761 acttgctgag caacatcccg acgacgcaac tgttgtcggc tacgcaaaaa gaattgttgg
     8821 tgaggcgacc gatttcgtga agcagcacaa tctcgtcact gttccggaag tgccgctcga
     8881 tgtgattgcg atgcccgaat tcaaacgcgg cgtcgccatc gcctactgcg attccccggg
     8941 accgctggag aagaacggca aaacatttta cgcagtcgca ccgacgccga aggactggtc
     9001 aaaggagcgg agagaatcgt tctttcgcga atacaacaat tatatgatcc gcgatttgac
     9061 cgtgcatgag gcgatgccgg gacattatct tcaaatcgcc cacgcaaatg agttcaaagc
     9121 gccgacgctg gtgcgcgcca ttttctccag cggcacgttt atcgaaggat gggccgttta
     9181 cacagaacaa atgatggctg agcagggtta cggcgggcca gaagtgaaaa tgcagcagct
     9241 aaaaatgcgg ctgcgcgcga tttgcaacgc gatgctcgac caaagcattc acgctggaaa
     9301 catgagcgag caggaggcgt tggatttgat gatgaaagaa ggctaccagc aggaaggtga
     9361 agcggtcgca aaatggaaac gggcccgact cacatcagct cagctttcca cttatttcgt
     9421 cggcgcaacc gagcatctcg atttgcgcgc cgccgaggag aagaaactag gcaaggattt
     9481 cgatctcaag aaatacaatg atcaggtgat ttcctacggc agcccgccgg tgaaatacgt
     9541 gcgtgagttg atgggattgt aggtggaacg cgttgtcttc aacgccttgg caagagcagc
     9601 gcaccttcag cggccaattt tatcatcgtc ttcggagaag acgatccacc tttgctttag
     9661 cggtatctga cgcaagattt atcaatgtct aatctcgcca catggatccg acggaaggat
     9721 gaaaaatggt ttcaaccatt ttttgcgaa
//
LOCUS       DASQUQ010000232        17062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1339789, whole genome shotgun sequence.
ACCESSION   DASQUQ010000232 DASQUQ010000000
VERSION     DASQUQ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 17062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17062
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339789"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VE486_06580"
     CDS             <1..1129
                     /locus_tag="VE486_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HZA38790.1"
                     /translation="SKLIIEEEKRLISVLPKLPAAREKRVLQALPSALGPRWAERALQ
                     LMQGPHGRMVAQIPRILSEAGRHTELRTMLERSIREHSATGEMLAWLCSERESWSELI
                     TPDLLGAIFAALEHEQHNAPGRASKLHRALVEDRQLLGDIYKKADVAIARDAMRRLQL
                     SPLFDELSKRSLLGRIVKAYPELEGMITGAQPEEKIAPLIVSWSSLERRKGEYEELVK
                     VKIPANTKEIALARSYGDLSENFEFKAAKQMQSVLMRRKAELEQMLHNARGTSFENVD
                     ASRVSIGTIVTLRSVENSKEETFTILGAWDGDPDRHIISYQTAIGQALLGHKAGDVVS
                     LNTEHGAAQFTIVSIQPAPPDESAPAPTLPRESAVEAAIAE"
     gene            complement(1136..1891)
                     /locus_tag="VE486_06585"
     CDS             complement(1136..1891)
                     /locus_tag="VE486_06585"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZA38791.1"
                     /translation="MHRFYIAPEQWNPDALALTESETHHARDVLRMKRGEKLVLFNGR
                     GREVTAEIVDLDSGEIRLRKLHEAETPPLRCRIVLGQAIPKGKNMELIVQKAVEIGAA
                     EIAPIISDRTVVQVDSESAAQKQAKWQQIAIEAAKQCGQNWLPRVHAPRKLAEFFDAT
                     APNTGFDLRLIGSLQPDAQRLKKILADYSSEHQERPQSVLMLIGPEGDFTPAELALAR
                     RHGCRPITLGPIILRVETAAIYCLSVLSYELLM"
     gene            complement(1948..2790)
                     /locus_tag="VE486_06590"
     CDS             complement(1948..2790)
                     /locus_tag="VE486_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZA38792.1"
                     /translation="MIECPRDAWQGLKKQIPTALKARYLGALIRAGFKHIDAVSFVSP
                     KAVPQMADSEKVLEQIDPPGDVEIIGIVVNERGTDRAIATEAVSTLGYPYSISETFLR
                     KNQNQTPEECYKVLQSIKMKADDSGLGMVVYVSMAFGNPYGDPWDEEKVSDAMKKVAG
                     LKIQSISLADTVGVADPDLIKRVVQSVTSQYANYDIGVHMHSTRNNASAKVLAAYDAG
                     CRRFDSAMGGLGGCPFAQDALVGNIPTESVIEALQQRGVQLPIGKSLEDVLALNSDIA
                     SKYE"
     gene            complement(2823..3959)
                     /gene="dnaJ"
                     /locus_tag="VE486_06595"
     CDS             complement(2823..3959)
                     /gene="dnaJ"
                     /locus_tag="VE486_06595"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZA38793.1"
                     /translation="MAIKKRDYYDVLGVGRNASEEEVKRAYRKLAVKFHPDKNPDDPH
                     AEEKFKELGEAYDVLMDSDKRAAYDRFGHDAFARGGAGFGGFHDPFDIFREVFGGGGF
                     GGGIFETFFGGAGARTEDRHRGSDLRYDLQIKLEEAAFGVEKEIEIEKLDTCDKCHGS
                     GAEPGSRTINCPTCGGRGQVISSRGFFHVSQTCPRCRGAGEIIEKPCPKCHGEGRVEK
                     LSRVKLKIPAGIRESSRLRSSHNGEAGIRGAPAGDLYVVIHIKEHKIFQREGDNLYCE
                     VPIPFSIAALGGEVEVPTLQGKAHLKVPAGTQSGQMFKLRGKGIININGRDRGDLLAR
                     LIVEVPSRLNAEQRRKLEEFAALCGEENTPLRKSFFERAKEFFK"
     gene            complement(4021..4569)
                     /gene="grpE"
                     /locus_tag="VE486_06600"
     CDS             complement(4021..4569)
                     /gene="grpE"
                     /locus_tag="VE486_06600"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZA38794.1"
                     /translation="MKKKQTEQPAPANPASAPAMPTGDVEQSELEDPMAGLQADLDRF
                     RDLALRSQADFENYKRRAAREKEEAIKYANSSLLERLVAIVDNFELGLEAAKGEGQQS
                     PVYSGMTLVLKQLSDFLAENGLQPIEAEGKKFDPNLHEAIAHQPSDFPEGTVIRQTRR
                     GYRFKDRLLRPSQVVVSSGPGK"
     gene            complement(4566..6002)
                     /locus_tag="VE486_06605"
     CDS             complement(4566..6002)
                     /locus_tag="VE486_06605"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HZA38795.1"
                     /translation="MWPTLLFGLLLASVAGWTFTAYYQTRKIRRLERSHEEIQVEETL
                     VFDFLHGLGEAFRETIRPQELHRLIVEGATRILDAHGGALYMTDRTGGKLTPVFISKG
                     CPPVVDVPPNILQQAAATPIALESYLRLHSVAPGEGLIGRVSQTGQSICLNDLSEVPE
                     LVKLRGSAFGTASVMASALSYGKQDLGVLAVANGPMGTPFSQGDFVVFKSIAEQSAFA
                     LYNAIIYSMANEKKRLDHDLEIARDIQRILLPAEAPAINGFQISGLNVPARQVSGDYF
                     DYIQVDDERLGVAIADVSGKGVPASLIMAICRSVLRAEAARNPSPADVLRKVNRQLYP
                     DIKEDMFISMAYLILDHQRDGITLARAGHDAPLLYKRQSQTVTPIKSPGMVVGIDSGN
                     VFDRLTADFAVPLERDDCLVLYTDGVTEALNSDGDEFGIDRMIQSVRASATDGAQTIV
                     KRIIEDVRNFTGSFPQNDDITLIAIRKT"
     gene            complement(6018..6533)
                     /locus_tag="VE486_06610"
     CDS             complement(6018..6533)
                     /locus_tag="VE486_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZA38796.1"
                     /translation="MESSIQVGVNGPTVWVKVEGKGSFLNSGNLKEFAREMLDRGYRE
                     FIIDLADCAMMDSTFMGTMASVALRLKELGRGHLHVVHCRNRSRELLSGLGLDQIFDI
                     HSNGATAPECEAIEGESRDQSRENKKQEQAQTMLEAHEALCEAAPENLFRFKDVLDYL
                     KQDLHHETPSK"
     gene            complement(6662..7273)
                     /locus_tag="VE486_06615"
     CDS             complement(6662..7273)
                     /locus_tag="VE486_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38797.1"
                     /translation="MSRFHYGMRLTPAAVVLLTIATGLARTVEEPRILDKTAPLPIAL
                     SKDFEFRKTKLYFLSDKPPKQSERARQTTSALKPGGASTSPSQKTLTLQDVPITFERQ
                     YRLFGAVTQVDQRQRFGDYFDFFWRAKRPADVTVRLEYRQEKLHDHVQAQEISYQNVR
                     GTRKTEFKVIGDDYLDDGRVIAWRCLLIENGRIVAENRSYMWE"
     gene            7381..10164
                     /locus_tag="VE486_06620"
     CDS             7381..10164
                     /locus_tag="VE486_06620"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZA38798.1"
                     /translation="MIDVRFERGVYLPQQELWLDPWDAKRFAFVSHAHSDHIAPHNEV
                     IVSERTARLMQSRLPGVRTEHVLPFGERRTVRRFDLMLLPAGHIFGSAQCYLFAGDET
                     LLYTGDFKLRPGKSAEQAEWRQADTLIMETTFGLPRYRFPPTEQVIQQVVNFCRETID
                     DDQVPVLLGYSLGKAQEILCSLEGAGLMPTLHRSVYQMTRIYEEFGQTFCKYVRYNPD
                     DVAGKVLICPPSANRSRMVETIPRKRVAILTGWAVDPNARYRYQVDAAFPLSDHADYP
                     DLIRYVELVRPRRVFTLHGFAAEFARDLRERGIDAWALNKENQMELALAPLGSARVSR
                     AGDGVLAVANFSSSSESAKRSRESKTGTRDAWATQSEFLAFANVGEVIAATPAKLEKI
                     RLLADYLRTLDQEQLPIATTYFTGRAFAQSDLRTLQVGGSIIYRAMMAASKLGDAEFH
                     RLAHSHGDAGKTAFEALQGRTRPEPFTLKQSRDFFDALHRARGPVAKTELLGSRLATL
                     SAREGQYVVKILSGDLRIGLREGLVEEAIARAFEVPLDEMKEASMLLGDTGATATLAS
                     RKELHRAELSIFRPIKCMLATPEPTAEAVWKRFVDEHSSDDANGAAATVYVEDKFDGV
                     RAQLHRNGERVEIFSRDLRRITDQFPELAERAKEFEQELIVDGEIIAFQEGRRLTFFD
                     LQKRLGRKSDVADLFEAAAADVPVAFIIFDLVWLNGRSLLKTPLRRRRELLGSLRLPR
                     QFQSAEVLPAHSAAEIEQLFQQARRRLNEGLMIKDPVSFYSPGRRGMFWFKLKKELAT
                     LDVVVVAAELGHGKRNNVLSDYTFAVRDGNTGELLPIGKAYSGLTDAEIAELTEHFQQ
                     NTIVDHGRYREVKPDIVLEVAFNSIQPSTRHASGLALRFPRIKAIRRDKNVDAIDTLE
                     YARQLAAERPNAE"
     gene            complement(10909..12759)
                     /locus_tag="VE486_06625"
     CDS             complement(10909..12759)
                     /locus_tag="VE486_06625"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HZA38799.1"
                     /translation="MKKYFGLPVIISLAIFCSCQKQQTEEERKAEVERQVQERLAAER
                     QAQSEQQLAQREADLEAREKALAAEKERAAESAPVAQALETPLRSSRTDISGNVATAS
                     YSTFYRKLEPYGVWRETPNYGYVWQPQETERSRSWRPYTNGRWVYTDAGWTWISEEPF
                     GWATYHYGRWTRLRNIGWVWVPGDEWAPAWVSWRKGNDYVGWAPLPPEAHFDRRSGIR
                     NWADNYYDIGPEQYCFVPTNEFGARRLETALVPVQRNVTIVNQTTNVTNITYSNTTII
                     NQGPSYDDLRSRSRQPIERLRLERQITANVEAETPRSVIRGDVVQIPAPVIATAQPAE
                     RPRKIKETVAQTVVERGWEGIADRQAAEKARAKIKSEATPPPDAPPKTLVRPTRATAE
                     TSPLVSRPVASPPAPSQQTPSVPSPKISAAAATASPTVPSRRGRPLTTASPARELVPS
                     PTVRPTPQLRQTPGAPPAITATPLHRATTPSHPHETPAVPPRPTLSPTHHLTPTVSSP
                     SPAATEVPTTPSTKTTNLRSTSSPPVADRSAENKDQPKAEGELRKQEPEAQGRQSKSQ
                     SGKARTRGADASPGQNATAPPVTSPSPTESPEKEEKKKRKRERAGAQENE"
     gene            complement(13068..13682)
                     /locus_tag="VE486_06630"
     CDS             complement(13068..13682)
                     /locus_tag="VE486_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZA38800.1"
                     /translation="MNVYYWLGYHLSRLVGRLFFRFRVIHRERMIQNGPVILAMNHQS
                     YLDPSLAGTTCDRPIYFLARKTLMDVPLLGWLLPKLNVIPVNQEGIDRSALKILIRVL
                     KSGNAALVFPEGSRTLDGNLQPAEPGLGLVIAKTLAPVVPMRIFGAHEALPRGGGGLH
                     FVPITIVIGEPIFFTTADVESPARDIYQRLSQRVMDAIAALHLK"
     gene            complement(13705..14373)
                     /gene="cmk"
                     /locus_tag="VE486_06635"
     CDS             complement(13705..14373)
                     /gene="cmk"
                     /locus_tag="VE486_06635"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZA38801.1"
                     /translation="MHSVPQSSHRVVAIDGPAASGKSSVARRLAQRLGFAYVNSGAIY
                     RAITWHILQKEIDTRDAHRVAQAAESAQIVCRLQNNESRVFIDNVDPADYLRDDRVNE
                     SVSHVSRVARVREIAVQKMRDCTRHHDLVVEGRDIGSIVFPGTPYKFYVDASPEVRLR
                     RRAAQGERDEIKMRDQADSSRAVSPLIIAPDASVIDTSHLTIDEVVNEIIARLEQNGL
                     HVSI"
     gene            complement(14455..15807)
                     /gene="aroA"
                     /locus_tag="VE486_06640"
     CDS             complement(14455..15807)
                     /gene="aroA"
                     /locus_tag="VE486_06640"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZA38802.1"
                     /translation="MELRVKRAPRIDIEIAVPGDKSISHRAVIIAALSNGICTLRGFL
                     PSEDCMHTVNAMRALGVKIEQPEPATLIVHGKKRVLTPPKKDIDCGNSATTMRLLAGL
                     LAGQTFESRLVGDAGVSKRPMDRVIAPLRQMGANIVAEGPEQTAPLRVGGGSLRGIHY
                     RLPIASAQVKSALLLAGLFARGRTTVEEPSPTRNHTEVLLNYFLVRTAQDDNGNITVF
                     GDQVPESRDFTIPGDISSAAFWLAAAAAQPRGHLLVRGVGLNHTRTAFLGVLLRMGAQ
                     VREAIEEVDQLEPRGIVEVTGVPLKGTVMQGKEVPQFIDELPILAVAGALANGQTIIR
                     HAQELRVKETDRIAAIAHNLRTMGAQVTEMNDGIQIYGPAPLRGARVASFGDHRIAMA
                     FAIAGLFADGETIVQDADCIRESYPGFATVLEGFTNPKRMQISTPVIGSLSPAPVEEA
                     "
     gene            complement(15934..16320)
                     /locus_tag="VE486_06645"
     CDS             complement(15934..16320)
                     /locus_tag="VE486_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38803.1"
                     /translation="MKFSLPLALFTAFFVASCAEEPPQRPPKRVATYSATQPQEYPPP
                     QQPFDPNGPVQPSGAPPVGTAPLPPPTAAPTKVAKGDYPYGIPVPGKPHLVESPYSPG
                     KYVDVEGFPPGTEVKDPYTDKIFLVP"
     gene            16469..>17062
                     /locus_tag="VE486_06650"
     CDS             16469..>17062
                     /locus_tag="VE486_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZA38804.1"
                     /translation="MSNNSRVQILVVDDDAMSRKILTQLLTSAGYQCDQCEDGAKALE
                     KIHASPPSLLLLDFDMPGLNGAEVLERLRSDNDPAIAQIPAIMLTGHGSEASEVRCLQ
                     AGANDFVTKPINDAVLRARIETQLRLRSMRRQLERQNDELEEWRHNLERDLAAARLTQ
                     QSLIPNKPPRLPGWQIATCYRPVIQVGGDIYGWLPMKE"
BASE COUNT         3635 a         4755 c         4849 g         3823 t
ORIGIN      
        1 ttcgaaattg attatcgagg aagagaagcg gttgatctcg gtcctgccga aattgccggc
       61 ggcaagagag aaaagagttt tgcaagcgct gccgtccgcg ctcggccctc gctgggcgga
      121 gcgggcgtta caattaatgc aaggccctca cggtcgtatg gtagcgcaaa ttccgcgtat
      181 cctgagcgag gccgggcgac acaccgagct tcggacaatg ctcgagcgga gcatccgcga
      241 acattccgcc acgggcgaaa tgcttgcctg gctctgtagc gaacgggaga gttggagcga
      301 gctgattaca ccggacctgc tcggggcaat tttcgcggcg ctcgaacacg aacaacacaa
      361 cgctcccggg cgcgccagta agttgcaccg tgcgctggtt gaagatcggc aattacttgg
      421 ggatatttat aagaaagccg acgtcgctat cgcacgcgat gcgatgcggc gtctgcaact
      481 aagcccgcta tttgacgagc tgagcaaacg ctcgcttctg ggccggatcg tcaaagctta
      541 cccggagttg gaaggcatga tcacgggcgc gcagccggag gagaagatcg cgcccttaat
      601 cgtttcctgg agcagcctgg aaagacgcaa aggcgaatat gaagaactcg tgaaagtgaa
      661 aatcccggcg aacaccaagg aaatcgcgct cgccaggtcg tatggagatt tgagcgagaa
      721 ctttgaattc aaggcagcca agcaaatgca aagcgtgctc atgcgacgca aagccgagct
      781 cgaacaaatg ctgcacaacg cacgcgggac gtcatttgaa aacgtcgatg cgtcgcgtgt
      841 ctcgatcggc acgatcgtca cgctgcgtag cgtggagaac agcaaggaag aaaccttcac
      901 cattctcggc gcgtgggacg gcgatcccga ccgccacatc atttcgtatc aaaccgccat
      961 tgggcaggca ttgctcgggc acaaagccgg cgacgttgtc tccctcaaca ccgaacacgg
     1021 cgccgctcaa tttacgattg tttcgattca gcctgcgcca ccggacgaat cagcgcctgc
     1081 cccaacgctt ccgagagaat ctgccgttga agcagcgata gctgaatgac aatcgctaca
     1141 tcagcaattc gtaggaaaga acactcagac aataaatcgc agccgtctcc acccgcagga
     1201 tgattggccc aagagtgatg gggcggcacc catggcggcg ggcgagcgca agttccgcag
     1261 gcgtaaaatc gccttccggc ccgataagca tcaaaacgct ttgcggacgc tcctggtgtt
     1321 cgctcgaata atcggcgagg attttcttta aacgttgcgc gtcgggctgg agtgatccga
     1381 tcaggcgaag atcgaatcct gtattcgggg ctgttgcatc gaagaactcc gcgagtttcc
     1441 tcggcgcgtg cacgcgcggc agccagttct ggccgcattg ctttgccgcc tcaatcgcga
     1501 tctgctgcca tttcgcctgt ttttgcgcgg cgctttcaga atcgacctgg acaacggtgc
     1561 gatcggaaat aatcggcgca atttcggctg cgccgatctc gactgccttt tgcacgatca
     1621 actccatgtt ttttcctttt ggaatcgcct gtcccaaaac gatccggcaa cgcagcggcg
     1681 gcgtttcggc ttcgtgcagc tttcgtaagc gaatttcgcc gctgtcgaga tcgacaattt
     1741 cggcagtgac ttcgcgcccg cgtccgttga acagaactag tttctcgccg cgcttcatgc
     1801 gcaggacatc gcgcgcatga tgggtttccg attcagtcaa cgcgagcgcg tcagggttcc
     1861 actgctcggg agcgatgtag aagcggtgca tcggcagatg tgacctatag gacaaatggg
     1921 ccctatggga caagcaatcc gagtacttta ttcgtacttg gacgcgatat ccgagttcag
     1981 cgccaggaca tcctccagcg atttgccgat cggcagctgc acaccgcgtt gctgaagcgc
     2041 ctcgattaca ctctctgtcg gaatgttccc aaccagcgcg tcctgcgcga agggacatcc
     2101 gccgaggcca cccatggccg agtcgaagcg gcggcaaccc gcgtcgtatg cggcgagcac
     2161 tttggctgaa gcgttattac gcgtgctgtg catgtgcacg ccgatgtcgt aattggcgta
     2221 ttggctcgtc accgattgga ctacgcgctt gatgagatcg gggtcggcga caccaacggt
     2281 atccgcgagc gagatggatt ggatcttgag gccggctact ttcttcatcg catcggacac
     2341 cttttcttca tcccacggat cgccatacgg atttccaaac gccatcgaaa cataaaccac
     2401 catgcccagc cccgaatcgt cagccttcat tttgatcgac tgcaaaactt tgtagcactc
     2461 ctcgggcgtc tggttctggt tctttcgcag aaatgtctca gagatcgaat agggataacc
     2521 aagtgtgctc acggcttcgg ttgcaatcgc gcggtcagtg cctctctcgt tcaccacgat
     2581 tccgatgatt tcgacatcgc ccggtggatc gatttgctcc agaactttct cgctgtccgc
     2641 catctgtgga acagctttgg gagagacgaa gcttacggcg tcgatgtgct tgaaccccgc
     2701 tcggatcagc gcgccaagat accgtgcctt tagtgctgtg gggatttgtt tcttaaggcc
     2761 ctgccaggcg tcgcgggggc attctatcaa ttttacggca tcagcaggca ccgttgagcc
     2821 cattatttaa aaaattcctt cgcgcgttcg aaaaagcttt tgcgcaacgg cgtgttctcc
     2881 tcgccgcaaa gcgcggcgaa ttcctcgagc ttgcgacgtt gttccgcatt gaggcgcgat
     2941 ggcacctcga caatcaagcg cgcaagcaag tcgccgcggt cgcgcccatt gatatttatg
     3001 attcccttgc cgcgaagctt gaacatctgc ccgctttggg tgccggcggg cacttttaag
     3061 tgcgctttgc cttgcagtgt cggtacctcc acttcaccgc ccaaggccgc gatcgaaaaa
     3121 ggaatcggca cttcacagta aagattgtct ccttcgcgct gaaagatttt gtgctccttg
     3181 atatggatga cgacataaag gtcgcccgca ggcgcgccac ggatcccggc ctcgccgttg
     3241 tgtgaggagc gaagtcgcga gctttccctg attccagccg ggattttcaa tttgacccgg
     3301 ctcagttttt ctacgcgtcc ctccccgtgg cacttcggac acggcttctc gatgatttcg
     3361 ccggcaccgc gacagcgggg acaggtttgc gaaacgtgaa aaaaaccgcg cgaactgatc
     3421 acctgaccac ggccgccgca ggtgggacag ttaatcgtgc gcgaacccgg ctcagcgccg
     3481 ctgccatgac atttatcgca ggtatcgagc ttttcgattt caatctcctt ttcgacacca
     3541 aacgccgctt cctccaattt gatctgcaag tcgtaacgca agtcggagcc gcggtgccga
     3601 tcctccgtgc gagcgccggc gcccccgaag aatgtctcga aaattccgcc tccaaatccg
     3661 cccccgccaa agacctcgcg gaaaatatcg aacggatcat ggaacccacc gaaaccagcg
     3721 ccaccccggg caaaagcatc gtggccgaag cgatcgtagg cggcgcgctt atccgaatcc
     3781 atgaggacgt cataagcttc accaagctcc ttgaattttt cctcggcatg cgggtcgtcc
     3841 gggtttttgt ccggatgaaa cttgactgcg agtttgcgat aagcgcgctt aacctcttct
     3901 tcggaggcat ttcgccccac gcccagcacg tcgtagtaat cgcgtttttt gatggccatg
     3961 tgacgaatga gtcctatgtg acctatgtga ctgatgggcg aggcaggcaa ggcgcctcac
     4021 ttattttccc ggcccgcttg aaacaacgac ctgcgagggt cgcagcaagc ggtccttgaa
     4081 ccgataaccg cgtcgagtct ggcggataac cgttccttcc ggaaagtcgc ttggttgatg
     4141 agcgatcgcc tcgtgcagat ttggatcgaa tttttttccc tctgcctcga ttggctgcaa
     4201 tccattttcg gccaggaaat cgctgagttg tttcagtacg agagtcatcc ccgaataaac
     4261 aggagactgt tggccttccc ctttggcggc ttcgagaccg agctcgaaat tatccacaat
     4321 cgccaccagc ctttcgagca acgagctgtt ggcgtatttg atcgcctctt ctttttcgcg
     4381 ggcagctcgc cttttgtaat tctcgaaatc agcctggctg cgcaatgcta gatcgcggaa
     4441 ccggtcgagg tcggcttgca acccggccat tggatcttcc agttcacttt gctccacgtc
     4501 acccgtgggc atggccggtg cggatgcggg gtttgccggt gccggctgtt ctgtttgttt
     4561 ctttttcatg ttttgcgaat ggcaatcaaa gtgatgtcgt cattttgtgg aaatgaaccg
     4621 gtaaaattgc gcacgtcctc gataattctt tttacaattg tctgggcacc atcggttgca
     4681 ctggcgcgaa ccgattggat catgcgatca atcccgaatt catctccgtc gctattaagc
     4741 gcttcagtca cgccgtcggt gtagagaacc aggcaatcat cgcgctcgag tggaacggcg
     4801 aaatcggcgg taagcctatc gaacacattt ccgctgtcaa ttcctacgac cattccaggc
     4861 gattttatcg gcgtaacggt ttgcgattgc cgcttgtaga gtaatggcgc gtcgtgcccg
     4921 gcgcgcgcga gcgtgatccc gtcgcgttga tgatccagga tgagataggc catgctgata
     4981 aacatgtctt ccttgatatc gggatagagt tgccggttca cttttcgcaa aacatcagcc
     5041 ggagaagggt tccgcgctgc ctcagcgcgc aacacgctgc ggcaaatcgc catgattagc
     5101 gatgccggaa cgccttttcc cgaaacgtcg gcaatcgcca cgccgagccg ttcgtcatcg
     5161 acctggatgt aatcaaaata atcgccgctc acctgccggg cgggtacatt aaggccgctg
     5221 atctgaaatc cgttaatggc tggcgcttcg gcgggaagca aaattcgctg aatatcccgc
     5281 gcgatttcga ggtcgtggtc gagccgcttt ttttcattcg ccatcgaata gataatggcg
     5341 ttgtagaggg caaaggcaga ctgttcggcg atcgatttga aaacaacgaa atcgccctga
     5401 gaaaatggtg tgcccattgg gccattggcg acggcaagca cgcccagatc ttgtttccca
     5461 taggagaggg cagaggccat aacggatgcg gtgccaaaag cgctgcctcg caacttcacc
     5521 agctccggca cttctgagag atcattcaaa caaatggatt gtcctgtttg ggagacgcga
     5581 ccgatcagcc cttcacccgg agcaaccgaa tggagccgca gataactctc aagggcgatg
     5641 ggggtagcgg ctgcctgctg caaaatattt ggcggaacat ccaccacagg tgggcagccc
     5701 tttgagataa agaccggcgt aagcttgccg cccgtgcgat cggtcatata aagcgccccg
     5761 ccatgtgcgt ccagaatgcg cgttgcccct tcgacgatta gccggtgcag ttcctggggt
     5821 cgaatggttt cacggaaggc ttcgccgagt ccgtggagga aatcaaaaac cagcgtttct
     5881 tcgacctgaa tttcctcgtg ggagcgttcc agccttcgga ttttccgcgt ctggtagtac
     5941 gcggtaaagg tccacccggc cacagacgcc agaagcagtc cgaaaagaag agtcggccac
     6001 atgatggaag ggccagctta cttcgagggc gtctcgtgat gaaggtcttg ttttaagtaa
     6061 tcgagaacgt ccttgaagcg aaaaaggttt tccggcgccg cctcgcaaag ggcttcgtgc
     6121 gcctccagca tcgtttgggc ttgctcctgt tttttgtttt cccgcgattg gtccctggac
     6181 tcgccctcga ttgcctcaca ttcgggtgcg gtcgcgccat tggaatgaat gtcgaaaatt
     6241 tgatcgaggc caagtcccga gagcagttcc cggctgcgat tccggcagtg aacgacgtgc
     6301 agatgacccc ggccaagttc cttcaaacgc aacgccacgc tggccatggt tcccataaac
     6361 gtggagtcca tcatggcgca atccgccaaa tcgatgataa actcgcgata accgcggtcc
     6421 aacatttcgc gcgcgaactc tttcaggtta ccgctgttca ggaaactacc tttcccctct
     6481 actttcaccc atacggtcgg accgtttacc cctacttgaa ttgatgactc cacgtttact
     6541 caggttaaag atacaggatc aagagccaga ctgtcaaacc tggccagtcc ccgggaccct
     6601 ttagaattga ttcgaattag agaagtcgga gagccgtatc tgccaaagaa gctggtctgg
     6661 ttcactccca catataggag cgattttctg ccacgatccg cccattttcg attaacaaac
     6721 atcgccaggc gatcactcgc ccgtcgtcaa gataatcgtc gccgatgacc ttaaactctg
     6781 tcttacgagt gccgcgcacg ttttgatagg aaatctcctg cgcctgaacg tggtcgtgta
     6841 gtttctcctg gcgatactcg agtcgcacgg tcacatctgc cggccgtttg gcgcgccaaa
     6901 agaaatcgaa gtaatcgcca aaacgctggc gttggtcaac ctgcgtcacg gcgccgaaaa
     6961 gccggtattg ccgttcaaac gtgattggca catcctgaag cgttagggtc ttctgggaag
     7021 gcgatgtgct tgcccccccc ggtttgagtg cactcgtggt ctgtcgtgca cgctcagatt
     7081 gtttcggcgg cttgtcactc agaaagtaaa gtttcgtttt ccgaaattcg aagtctttgc
     7141 ttaacgcgat cgggaggggt gctgtcttgt cgaggattct tggttcttcg actgttcggg
     7201 cgagcccggt tgcgatagtg agcagcacaa ccgctgcggg ggtaagtctc atcccgtaat
     7261 gaaaacggct cacgcgttcc atgtcaatat acgacatatg cgacatatga gtccgatggg
     7321 taaacagaca gatgtctgtg ttgcgttttc tttgcgcgac tccgcgggta aattttcccc
     7381 gtgattgatg ttcgttttga gcgcggggtt tatctgccgc agcaggaatt gtggctcgat
     7441 ccctgggatg cgaaacgttt cgccttcgtt tcacacgcgc acagcgacca tattgcgccg
     7501 cacaatgagg tcatcgtttc tgagcggact gcgcgtttga tgcaaagccg gcttccgggc
     7561 gtgcggacag agcatgtcct gccatttggt gaaagacgaa cggtgcgcag gttcgacctc
     7621 atgttgctgc ccgcaggcca catctttggt tcggcacagt gttacctttt tgccggggat
     7681 gagacgctgc tttacacggg tgatttcaaa ctgcgaccgg ggaaatccgc tgagcaggcg
     7741 gagtggcgtc aagccgatac gctcatcatg gaaacgactt ttggcctgcc ccgctatcga
     7801 tttccgccca ccgagcaggt gatccagcag gtagtcaatt tctgccgcga gacaatcgac
     7861 gacgatcagg tgccggtgct gctgggttac tccttaggca aggcgcagga aatattgtgc
     7921 tcgctcgagg gagccggcct catgcccacg ctgcaccgtt cggtctatca aatgacgcgc
     7981 atttatgaag agttcggcca gacgttttgc aaatatgtgc gctacaatcc cgatgatgtt
     8041 gcggggaaag tcctgatctg cccgcccagc gccaatcgat cgcgcatggt ggaaacaatt
     8101 ccgcgcaaac gggtcgcaat cctcaccggg tgggcagtcg atccgaatgc acgttatcgc
     8161 tatcaggtcg atgccgcatt tccgctctca gatcacgctg attaccccga cttgattcgc
     8221 tatgtcgagc ttgtgcgacc ccgacgcgta tttactcttc acggtttcgc cgcagaattt
     8281 gcgcgcgact tgcgcgaacg cggcatcgac gcctgggcgc taaataaaga aaatcaaatg
     8341 gaactggcgc tggcaccttt gggtagcgca cgcgtctcgc gtgctggcga cggcgtcctc
     8401 gccgtcgcga acttttcttc cagctccgaa tcagcgaaaa gatcgcgaga gtccaaaacc
     8461 ggcacaagag acgcgtgggc tacgcaatcc gaattcctgg cgtttgccaa tgttggggaa
     8521 gtgattgctg caactccagc aaagctggag aaaattcgac tgctcgcgga ttatttgcgc
     8581 accctcgatc aagagcaact tcccattgcg acgacttatt ttaccggccg cgcatttgct
     8641 cagagcgatc tccgcacctt gcaagtggga ggatcgatta tttaccgtgc aatgatggcg
     8701 gcatcgaaat tgggcgacgc cgaatttcat cggctggcac acagccatgg cgatgcgggc
     8761 aaaacggcct tcgaagcctt gcagggacgc accaggcccg aaccattcac gctgaagcag
     8821 tcgcgtgatt tctttgatgc gttgcacagg gcgcgtggtc cggtcgccaa aaccgagctg
     8881 ctgggatcgc gcctggccac gctttctgcg cgcgagggtc agtacgtggt caagatcctc
     8941 agcggagatt tacgcatcgg tttgcgggag gggctcgtcg aagaagcgat tgctcgcgca
     9001 tttgaggtgc cgctcgatga aatgaaagaa gccagcatgc ttctgggcga caccggtgcc
     9061 accgcaacgc ttgcctcccg aaaagaattg catcgtgcgg agctgtcgat ctttcgtccc
     9121 atcaagtgca tgcttgcgac tcccgaaccg acagcagaag cggtgtggaa acggtttgtc
     9181 gatgaacact catccgacga tgccaacgga gcggcagcca ccgtttacgt agaagataaa
     9241 ttcgacggcg ttcgcgcaca gcttcatcgc aacggagagc gggtggaaat tttttcgcgc
     9301 gacttgcggc gtatcaccga tcaatttcca gagctggcag agcgagcaaa ggaattcgag
     9361 caagagctga ttgtggatgg tgaaattatt gccttccaag agggtcggag gcttactttc
     9421 ttcgatttac aaaagcggct cggccggaaa agcgatgtcg cggatttatt tgaagcggcc
     9481 gcggcggatg taccggtcgc attcattatt tttgatcttg tttggctgaa cggacgctcc
     9541 ttgttgaaaa ctccgctacg acgccggcgc gaattgctcg gcagcttgcg tctcccgcgg
     9601 cagttccaaa gtgccgaagt attgcccgca cattctgccg ctgaaatcga gcaacttttc
     9661 caacaggctc gccgtcgttt gaatgaaggt ctgatgatca aggacccggt gagtttttat
     9721 tcgccggggc gtcggggaat gttctggttc aaactaaaga aggaattggc gacgctggat
     9781 gtggtggtgg tcgccgccga gcttggccac ggcaaacgca ataatgtgtt gagcgattac
     9841 acatttgcgg tgcgcgatgg aaacaccggt gaactcctgc ccatcggcaa agcctacagc
     9901 ggattgaccg acgcggagat tgcggaatta accgagcact ttcagcaaaa taccatcgtg
     9961 gatcatggcc gatatcgtga agtgaaaccg gacatcgtgc tcgaggtcgc atttaattcg
    10021 atccagccga gcacgcggca tgcgagcgga ctcgcgctcc gcttcccaag aatcaaagcg
    10081 atccggcgcg acaaaaatgt ggatgcaatc gacacgctcg aatacgcgcg acagctcgct
    10141 gccgagcgac cgaacgctga gtgacggcgt aatccagcca gctggcggga gtgctccccg
    10201 actcgtctgc ggcgctactg cgctgttccg tcgggggatt ctggcgttgg cgtaggggaa
    10261 gcgctttctg ccgcttcagg caacggcgag acggccggcg atgccgcctc ggcgggtgtc
    10321 accgaaggag tccctgaggt ttccgaaggg actggtaccg atgcttccgg cattgggctg
    10381 gcaaaacgac tccgtgcaga tggacgattc cgaagccccg gaatcgggtg cggggttcgg
    10441 acggcccccg gtgctaaacc tgtcgccgga tttgccgatc tttcaccgat cgacggacgg
    10501 ctcgggactc gtgaaactcc aggcacctgg gtgttccctg cggcttttct cttctcaagc
    10561 agcgcattcc tgcgggcagt gaattcggcg cggcgtcgtt gagcttcctg ttcctgagcc
    10621 tcatgttccg cggctaatcg tcgctgcact tcctgctcga ttcgggcctg tatttctgct
    10681 tccgttggat gcttctggca ggagcaaaat atggcaaggg caacaatcac cggcagagca
    10741 aaaagttttt tcatgtcagg atcggattgt gatcggctct tatcgtttca acgaggcgaa
    10801 taagcaagca cgcagccgag cgcgagtaaa tgcgtcggcc cagatgaccg acctgcaagc
    10861 gtaacatcga tctcgaccct tgccgtaacc acatcgattg atcgaggcct actcgttttc
    10921 ctgggccccc gcacgctcac gtttacgctt cttcttttct tctttctccg gcgattcagt
    10981 gggagacggc gaagtcacag gcggcgccgt cgcgttttga ccgggagatg catctgcgcc
    11041 gcgtgttctc gcttttccgg actgactctt gctctggcgt ccctgcgcct ctggctcttg
    11101 ttttcttaat tctccctcgg ccttgggctg atccttattt tcagccgaac ggtcggccac
    11161 cggcggagac gacgtcgatc gtaagttcgt ggtcttcgtc gaaggagtag ttggaacttc
    11221 agtcgcggct ggtgagggtg aagagacggt cggggtcaga tggtgtgtcg gggaaagcgt
    11281 tggtcgcggc ggcactgcgg gtgtttcgtg tggatgcgaa ggtgtggtgg cccgatgtaa
    11341 aggggttgct gtgattgcag gaggcgcccc aggcgtttgg cgcaattgtg gcgtcggcct
    11401 tactgtggga gacggaacta attcccgggc cgggctggca gttgtaagag gtctgccacg
    11461 gcgcgatggc acggttggtg aggccgttgc ggcagctgcc gaaattttgg gtgacggaac
    11521 tgatggagtt tgctgcgacg gcgccggtgg gcttgccacg ggtctcgaga ctagtgggga
    11581 cgtttcggcg gttgcccgtg ttggtctcac gagagttttc ggcggagcat ctggcggagg
    11641 cgtggcttcg gatttgatct tcgctcgcgc tttttcagct gcctgccgat ccgcgattcc
    11701 ttcccagcca cgctccacca ccgtctgcgc gactgtttcc ttaatcttcc gcggccgttc
    11761 agccggctgc gctgtagcga ttaccggagc cggaatttga acaacgtcac ctcggataac
    11821 cgagcgtggc gtttctgctt ccacgttcgc tgtgatctgg cgctcgagac gcaggcgttc
    11881 aatcggctgc cggctgcgtg aacgcaggtc gtcgtagctt ggaccctgat ttatgatggt
    11941 cgtgttactg taagtgatat tggtgacgtt tgtcgtctga ttaacgatcg tcacgttccg
    12001 ttgcaccggc acgagcgctg tctcaagacg gcgcgcgcca aattcattcg tcggaacgaa
    12061 acagtattgc tccggaccga tatcgtaata attgtcagcc cagttacgaa taccggagcg
    12121 ccgatcaaaa tgcgcttcgg ggggcagcgg cgcccaaccg acataatcgt tacctttgcg
    12181 ccacgacacc cacgctggcg cccattcatc tccggggacc caaacccatc caatgttgcg
    12241 caaccgcgtc cagcgcccgt aatggtaggt tgcccagccg aaaggctctt ccgatatcca
    12301 ggtccagccc gcgtcggtgt aaacccagcg gccgttggtg taaggacgcc acgaccgcga
    12361 tcgctctgtt tcttgcggtt gccagacata gccgtaattc ggggtctcac gccacacgcc
    12421 atacggctca agtttcctgt aaaacgtcga ataggacgcg gtggctacat ttccggaaat
    12481 gtccgtccgg gaagaccgca gtggtgtctc caaggcttgg gcgacgggtg cgctctctgc
    12541 ggctcgttcc ttttcagccg ccaatgcttt ttcacgcgct tccaggtccg cttcccgctg
    12601 agcgagttgt tgttcactct gggcttgtcg ttccgcagcc agacgctctt gtacttgtcg
    12661 ctcaacttca gccttccgtt cctcctcggt ttgctgcttt tggcaggagc aaaatatcgc
    12721 taaagaaata atcaccggca ggccaaaata ttttttcatg tcaggattag accaccattg
    12781 atttgggtta ttcgaacgag gcaaataaaa cctccggtga ctcgaaggag aatcaaggcg
    12841 caggcgccgg cggggaaggt gctggaatca cgactacggt ttcgacctgc gcccgccgca
    12901 aacgctggac attggaatac gcagccagga gcgccattga tccgaggatg gtgagcataa
    12961 gcacaatacg actcctggga actcgggatg gtggtcgcgc ttcgccagct ggatgcattg
    13021 aattcacctt tgaatatcga tgtccggctt gcgcgcgcaa tctgcattca ctttaaatgt
    13081 aatgccgcga tcgcatccat gacgcgttgg cttagtcgct ggtaaatgtc cctcgctggc
    13141 gattcgacgt cggcagtcgt aaaaaaaatc ggttctccga tcacgatggt gattggcaca
    13201 aagtgcaaac caccaccgcc acgcggcaag gcttcgtggg cgccgaagat tcgcatcggc
    13261 acgaccgggg cgagcgtttt tgcgatcacc agccccaagc ccggctctgc cggctgcaaa
    13321 ttaccatcga gcgtgcgcga gccttccggg aaaaccagcg ctgcgtttcc agacttgaga
    13381 acgcgaatca ggatcttgag cgcgctacgg tcgattcctt cctgattaac cgggatgaca
    13441 tttagcttgg gcaggagcca gccgagcagc ggtacgtcca tcagcgtctt ccgggccagg
    13501 aaatagatcg gccgatcgca tgtcgtgccg gcaagcgacg gatcgagata gctctggtga
    13561 ttcatggcga gaattaccgg accattttga atcatccgct cccgatgaat tactcgaaat
    13621 cgaaaaaaca atctgccaac caggcgagag agatggtatc cgagccagta gtaaacgttc
    13681 ataatcgaag cgttgaagcg acggtcagat ggaaacgtgc aggccgtttt gctcgagtcg
    13741 ggcaatgatc tcgttgacga cttcatcgat tgttaaatgc gacgtatcga tgacggacgc
    13801 atccggcgcg atgatgagcg gtgagacggc ccgcgaagaa tcggcctggt cacgcatttt
    13861 aatttcatca cgctcgcctt gcgcggcacg acggcgtaaa cgcacctctg gcgaagcatc
    13921 gacgtaaaat ttatagggag tgcctggaaa gacgatggaa ccgatgtccc gaccctccac
    13981 gaccagatcg tgatggcgcg tgcaatcacg cattttttgc accgcaattt cgcggacccg
    14041 ggccaccctg ctgacgtgag agacactttc gttcacgcga tcgtcacgca gataatccgc
    14101 tggatcgacg ttatcgataa agacgcgcga ctcgttgttt tgaaggcggc agacgatctg
    14161 ggcagactcg gcagcctggg cgacgcggtg ggcgtccctc gtgtctatct ccttttgcag
    14221 aatatgccag gtgattgccc gatagatcgc tcccgaattc acgtaggcaa aacccagtcg
    14281 ctgggccagc cgtcgcgcga cgctactttt tccggaggcg gccgggccat caatagcgac
    14341 gacgcgatga gaagattgcg ggacagaatg catgacgcga ggcaacttta gcaatcgttc
    14401 tcatggcgac gcaatagcct cagaggtcgt atgtgaccta tgtggtcttg tgaattacgc
    14461 ttcctcgacc ggagcgggac tgagcgagcc gatgacgggc gtgcttatct gcatccgttt
    14521 cgggttggtg aacccttcga gcaccgttgc aaatccggga taagattcgc gaatgcagtc
    14581 tgcatcctga acgatggttt cgccgtcggc gaacaaaccg gcaatggcaa aagccatcgc
    14641 gatgcgatga tcgccaaagc tggccacgcg cgccccgcgc aacggagccg ggccatagat
    14701 ttggatacca tcattcattt cagtgacttg cgcgcccatc gtgcgcaaat tgtgcgcgat
    14761 cgcagcgatg cgatcggttt cttttacgcg caattcctga gcatgacgaa taattgtctg
    14821 gccatttgcc agagcgcccg cgactgcgag gatgggcagt tcgtcgataa actgcggtac
    14881 ctctttgccc tgcataacgg tgcctttgag gggaacgccc gtgacctcca cgattccccg
    14941 gggttccagt tgatcgactt cttcgattgc ctcccgcacc tgcgcgccca ttcgcagtaa
    15001 aacacctagg aacgccgttc gtgtgtggtt caggccaact ccgcgcacga gcagatgccc
    15061 ccgcggttgc gccgcggcag cggccaacca aaaggccgcc gatgaaatat cgcccggaat
    15121 ggtaaagtcg cgcgactcag gaacctggtc gccgaagacc gtgatgttgc cattatcgtc
    15181 ctgtgccgtg cgaacgagaa aataattaag cagcacctct gtatgatttc gcgtaggtga
    15241 tggctcctct acagtggtcc tgcctctggc aaaaaggccc gccagaagca acgcgctctt
    15301 gacctgcgcg ctcgcgatgg gcaggcgata atgaatccca cgcaaggagc cgcccccaac
    15361 acggagcggc gccgtctgtt ccggcccttc tgcgacaatg tttgcaccca tctggcgaag
    15421 cggtgcaatt acgcgatcca tgggacgttt ggagacaccg gcatcaccca ccagcctgct
    15481 ctcaaaggtt tgtccggcaa gcaggccagc aaggagtcgc atggtcgtgg cggaatttcc
    15541 gcaatcaata tcttttttgg ggggggtaag cacccgcttt ttcccgtgga caatcagcgt
    15601 ggccggctca ggctgttcga tcttgacccc caaagcgcgc atcgcattga ccgtatgcat
    15661 gcaatcttcg ctcgggagga agccgcgcaa tgtgcatatg ccattggaca aagcggcgat
    15721 gataacagcg cgatgcgaaa tgcttttatc gccgggtaca gcaatctcga tgtcgattcg
    15781 aggtgcccgt tttacccgca actccataga tcaatgaaag aatgcctggc ggtcgatgct
    15841 cgataaaaaa aacagcgcgg cgttaatcca gtatccagta tccagtatcc agcatcagcc
    15901 aaaacccgct aatgaatccg ggcggcaacc ccgttatgga acaagaaaaa ttttgtctgt
    15961 gtaaggatcc tttacttccg ttcccggggg gaatccttcc acatcaacgt atttgccagg
    16021 tgaataggga ctttccacca aatgaggttt gccagggacg ggaattccgt agggatagtc
    16081 tcccttcgca actttcgttg gagcagccgt cggcggaggc aaaggggcag tccccacagg
    16141 cggcgcgccg gaaggctgaa caggtccatt tggatcgaac ggctgttgcg gcggcgggta
    16201 ttcctggggc tgggttgccg agtaagttgc aacgcgttta gggggacgtt gcggcggctc
    16261 ttcagcgcat gatgcaacaa agaaagcggt gaacagggcg agcggcagtg agaatttcat
    16321 aaagtaggat tgctctcaca acgtatccac ggtccgattc attgccaatc actaaatcgc
    16381 tattctcttc aagtcattgc gtcggcaaag atcgaaacga atagagaata caataaccgg
    16441 gcgctctcca tccgaatcca aagaagagat gtcgaacaac tctcgtgtcc agattttagt
    16501 ggtggatgat gacgccatga gtcgcaaaat cctgacgcaa cttctgacgt cggccggcta
    16561 ccagtgtgac caatgcgagg acggggccaa agcgttagaa aaaattcacg ccagcccgcc
    16621 ttcgctgctt cttcttgatt tcgatatgcc aggtctaaac ggcgctgaag tgttagaacg
    16681 ccttcgctcg gataatgacc cggcaatcgc acaaattccc gcgatcatgt tgaccgggca
    16741 cgggagcgaa gcaagcgaag tacgttgttt gcaagcgggc gcgaatgatt tcgtgacaaa
    16801 accgatcaat gacgcagtgt tgcgggcgcg aatcgagacc cagcttcgcc tgcgttccat
    16861 gcggcgccaa ttagagcggc aaaacgatga actcgaagaa tggcgtcata atctcgagcg
    16921 cgatttggcc gcggcgcgcc tgacccagca atcgctgatt cccaacaaac cgccgaggct
    16981 gcccggttgg cagattgcca cgtgttatcg cccggtaatc caagtgggcg gcgatatcta
    17041 cggttggctg ccgatgaagg ag
//
LOCUS       DASQUQ010000233         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_334639, whole genome shotgun sequence.
ACCESSION   DASQUQ010000233 DASQUQ010000000
VERSION     DASQUQ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334639"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..2130)
                     /locus_tag="VE486_06655"
     CDS             complement(94..2130)
                     /locus_tag="VE486_06655"
                     /inference="COORDINATES: protein motif:HMM:NF036774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HZA38805.1"
                     /translation="MKSGNFFAELKRRNVYKVAVAYIVAGWALAQGIAQVFPVFDVPN
                     WVVRLIVALIIIGFPIALIFAWALEITPEGLKRAEEADAMPPSARPTNRTWIYIVVIG
                     AVVSLALFFLGRYTAGDRTTARDDNKSIAVLPFESLSQDKNDAYFADGVQDQILTNLA
                     KVSDLKVISHTSVRQYKTGAERNLREIGQQLGVAYIVEGSVQRARDRLRINATLIDAR
                     TDAHVWAETYDRTAADLFAIQSELAESIVTQLKAKLSPRQKAEIEERPTQDLVAFELY
                     LQAKAIIDSYLNATDVRATLLQALHSLDEAIKRDPNFVSAYCYAARANDLLFFFDLDP
                     TPNRILLADAAVKAALRLRPDSAEAHFALADYLFRCQRDYNRALEELAIARPGLPNNT
                     PFLILSGYITRRRNHFHEAERDFSTAVALDPRNPNAYNLLADTYVLQRRFPEAVQVYD
                     RVLAAGEQFPIVRFRRASATFYGTSNSGPLREILAKAPEMDVAGGQTPARVMLAIIDG
                     NYAEAERVLAASPLQDFQDIDFSFYYPKAWFEAMIARAKGDSAGAIAAFRECRAILEQ
                     RLVVKPEHARTIAVLAQVDAGLGQKELAVREAQHAIDLMPISRDIYDGALVLEGLAQV
                     YTWSGERDRAIELVQKLVSMPSYINYARLKLHPLWSPLRGDPRFEKIVNSLAPK"
BASE COUNT          605 a          715 c          740 g          568 t
ORIGIN      
        1 gcgaagagat ctgcggaccc tgtgaattga gcatccaacg attcctgtcc aatcggcact
       61 tcaaaaattg taagtagaag acggcagcag atcttatttc ggcgccaaag aattcacgat
      121 tttttcaaat cgcgggtcgc ctcgcaacgg gctccacaac gggtgtaatt tcaaacgggc
      181 gtaattgata tagctcggca tcgacaccag cttctgcact aactcaatgg cgcgatcgcg
      241 ttcaccgctc catgtgtaaa cctgggcgag accttcgagg accagagcgc catcgtagat
      301 gtccctcgag atcggcatca aatcgatcgc gtgttgcgct tcgcgaacgg ccaattcttt
      361 ctggccaagt cccgcgtcga cttgagcaag gacagctatg gtccgcgcgt gctccggttt
      421 gacgaccaat ctctgttcca ggatcgcacg acattcgcga aaagcagcga ttgccccggc
      481 agaatcacct tttgccctcg caatcatggc ctcgaaccag gccttgggat agtagaagct
      541 gaagtcgatg tcttgaaagt cctgcagggg cgaagcggca aggacccgct ccgcctcagc
      601 atagttgccg tcgataatcg caagcatgac ccgggcaggc gtctgtccac cggcaacgtc
      661 catttctggc gccttagcta gaatctcgcg cagcggtcca gagttggatg tcccgtaaaa
      721 tgtggctgac gcccttcgga accgaacgat cgggaactgc tctccggcag cgaggacacg
      781 gtcgtaaacc tgcaccgctt cggggaacct gcgctgcagc acgtaggtat cggcaagaag
      841 attataggcg ttgggattcc gcggatcgag agcgacggcg gtggagaaat cgcgttccgc
      901 ctcgtgaaaa tggttgcggc gtcgagtgat gtagccggag agaatcaaga acggcgtgtt
      961 attcggcaga cctggacgcg cgatggcaag ttcttccaga gcgcgattgt agtcgcgctg
     1021 acatcggaac agataatcag ccagggcgaa atgcgcttca gctgaatcag ggcgaagtcg
     1081 cagggctgcc tttaccgcgg catcagcaag gagaatgcgg ttcggggttg gatcgagatc
     1141 gaaaaagaag agcagatcgt ttgcgcgcgc ggcataacaa tatgcagaga cgaagttcgg
     1201 gtcccgtttg atcgcctcgt cgagcgaatg cagcgcctgc agcaatgtcg cccgcacgtc
     1261 tgtcgcatta agatagctgt cgatgattgc cttggcttga agataaagct caaacgccac
     1321 cagatcctgc gtagggcgct cttcaatttc cgctttctgt cgtggcgaaa gtttcgcttt
     1381 gagctgagtg acgatcgatt cggccagctc gctctggatc gcgaacaaat cggccgccgt
     1441 gcgatcgtag gtttccgccc agacgtgagc gtcggtgcgc gcgtcaatca atgtcgcatt
     1501 gatacgaagc cgatcacggg cgcgctggac cgaaccttcc acgatgtaag cgacaccgag
     1561 ctgctgacca atttcgcgca gattgcgttc ggccccggtc ttgtattgac gcacgctagt
     1621 gtggctgatg actttcaggt cggagacctt ggccaaattg gtcaggatct gatcctggac
     1681 tccatcggcg aaataagcgt cgttcttgtc ctggctcagg ctttcgaagg gcaataccgc
     1741 gatcgatttg ttatcgtcac gtgccgttgt tctgtctcca gcggtgtaac gacccagaaa
     1801 aaacaaagcc agggagacaa cggcgccgat cacaacgata taaatccagg tgcgattcgt
     1861 tggccgcgcg gacggcggca tggcatcggc ctcttcggcg cgcttgagcc cttcaggtgt
     1921 gatctctaat gcccacgcga aaataagcgc gatggggaaa ccgatgataa ttaaggccac
     1981 aatcagtcgg acgacccaat tcggaacatc gaatactgga aaaacctgtg cgattccctg
     2041 agcaagcgcc cagccggcga cgatgtaggc gaccgcgact ttataaacat tgcgccgctt
     2101 cagctcggcg aaaaagttgc ccgacttcat cgctttggtg tagtgccaca gggtcagcgg
     2161 gagagcaaga gactcccggg atcggctcgg tttccgagat tcataaggct ccgccgcaac
     2221 gagaacattt aaccgcggat aacgcggata atttcagatg aattggagtc cgccaaacac
     2281 gccaaacgac gccaaaaaag aaaggctgag gttctgattc ccaatcttta gcgtcttttg
     2341 gcgtatttgc cggatcaacc ctgctttttc ctatctccat ccatgtgatc cgtgtaacct
     2401 gtcacgccgt agccttgcgc gtaggcggat ccgtggttta gagcttggtc cttcatggcg
     2461 tagtattccg ccggatcgcc ggcgaagaga tctgcggacc ctgtgaatta agcatccaac
     2521 gattcctgtc caattcacga ttttttcaaa tcgcgggtcg cctcgcaacg ggctccacaa
     2581 cgggtgtaat ttcaaacggg cgtaattgat atagctcggc atcgacac
//
LOCUS       DASQUQ010000234         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_55520, whole genome shotgun sequence.
ACCESSION   DASQUQ010000234 DASQUQ010000000
VERSION     DASQUQ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55520"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(371..931)
                     /gene="frr"
                     /locus_tag="VE486_06660"
     CDS             complement(371..931)
                     /gene="frr"
                     /locus_tag="VE486_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZA38806.1"
                     /translation="MSRDDILLEAEMSMEKSVDYMVHEFAAVRTGKASPGLVENVDVQ
                     AYGSTMKLKQLALITTPEPRLLVVQPFDAGTVPDIERALKESKIGIMPAVDGKIIRLP
                     IPELSEERRKDLARSLGKMAEEARVRVRSNRRGALDEAKKLKAAGELSEDGLHDLEAE
                     VQKLTDRFVKSVDDHLKHKEAEIMRV"
     gene            complement(944..1684)
                     /gene="pyrH"
                     /locus_tag="VE486_06665"
     CDS             complement(944..1684)
                     /gene="pyrH"
                     /locus_tag="VE486_06665"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZA38807.1"
                     /translation="MQTETPAYKRIVLKLSGEALQERGARDNISMPVVREIAERIKEV
                     RDLDVQVSIVIGGGNIWRGLSASHRGMDRTTADYMGMLATVINALALRSTLAQIDVET
                     RVQTAIEMKNVAEPFILGRAIRHLELGRVVIFAAGTGNPYFSTDTTAALRASEIRADV
                     ILKATKVAGVYDRDPNTDPDARLYDRITFTEALTKRLQIMDSTAFSLCMDNKIPIVIF
                     DMNKPSNIRDAVLGRKVGTLVCDEPQPN"
     gene            complement(1892..>2155)
                     /locus_tag="VE486_06670"
     CDS             complement(1892..>2155)
                     /locus_tag="VE486_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38808.1"
                     /translation="AEIIERKIRAFNPWPGAFMKLGERNLKIFSASIVDVGGKPGEIL
                     RSDKELVIAAGKDALSVGEVQLEGKRRMDIAEFLRGHIRRGAK"
BASE COUNT          424 a          624 c          561 g          546 t
ORIGIN      
        1 gtgccagttt ctggtcgatc tcgtgcggtt gtaagaccca agcaaaatcg agtgagctgt
       61 aggatgcgta gttctcgact tactctaact tgtcatgtgc gttatacccg cggagtcttc
      121 tttgctcgcg cgcagtctct ctccatgccc agatttcaat tttgatgcga gcgatcaaac
      181 gacggatgcc ccgctgactc tgccactcgt gcgccctctg tgccaacaac cggaagagga
      241 ttctgtcgtg ctccttaact tttcggataa gatcgatagg atttcgggta gcaattatct
      301 gcatgagcgt ggtgtcatct gaaaatcgta gtacgcaaag actttttctg cctgtttctg
      361 gttggttcgc ttagactctc atgatctcgg cttctttgtg cttgagatga tcatcaacgg
      421 atttgacgaa gcgatcggtc agcttttgca cttcggcttc caaatcgtgc aatccatctt
      481 cgcttaattc gccagcggct ttgagtttct tggcttcgtc gagtgccccg cgccggttgc
      541 tccgcacgcg gacgcgcgct tcctccgcca ttttgccgag cgaacgcgcg aggtccttgc
      601 ggcgctcttc cgataattcc ggaatcggca gtctgataat ctttccgtcg accgccggca
      661 tgatgccgat ctttgattcc ttcagggctc tctcgatatc cggcaccgtg cccgcatcga
      721 aaggctgaac aacgagcaaa cgcggctccg gtgttgtgat caacgcgagc tgcttcagct
      781 tcatggtcga gccgtaggcc tgcacatcga cattttccac gagcccgggc gaggctttgc
      841 cggtgcgcac ggcagcaaat tcgtgcacca tataatcgac acttttttcc atcgacatct
      901 cggcctccaa tagaatgtcg tctctgctca tgttgctgaa tttttagttc ggttgcggct
      961 catcgcaaac cagggtcccg acttttcgcc ccagcacggc atcgcggata ttgctcggct
     1021 tgttcatatc gaaaatcaca atcgggatct tattgtccat gcaaaggctg aacgctgtcg
     1081 aatccatgat ctgcaatcgt tttgtgagcg cctcggtaaa tgtgatgcga tcgtagagtc
     1141 gcgcgtctgg gtcggtattt ggatctcgat catatacgcc ggcgactttg gtggctttca
     1201 ggatgacgtc cgcgcgaatc tcgctcgccc gcagcgccgc ggtcgtgtca gtggaaaagt
     1261 aggggtttcc ggtcccggcg gcgaaaataa ctacgcgccc aagttcgaga tggcggatgg
     1321 cgcgtccgag aatgaaaggc tcggccacat ttttcatttc aatggccgtt tgcacccggg
     1381 tctcgacgtc tatttgcgcc agggtgctgc gtaatgccag cgcgttgatc acggtggcga
     1441 gcatgcccat gtaatcggcc gtcgtgcgat ccatcccacg atgcgacgca gagagaccac
     1501 gccagatatt tcctccgcca atgacgatcg acacctggac atcgagatcg cgcacctcct
     1561 ttatgcgttc ggcaatttca cgcacaaccg gcatcgagat gttgtcgcgg gcgccgcgct
     1621 cctggagcgc ttcgccgctc aacttgagaa cgatccgttt ataagctggc gtctccgttt
     1681 gcatggtcgt gcataatgat caaacagaag atgtcgatct tgaaaagcac aaacgcgaac
     1741 tgatggggga gcgcgggcat tttgcccgct gccgccggca tgttgccgcc ggctcgctgg
     1801 aatcgcaacc gaagcaatcc gccacgctgc tgattactgc gcaacacgct cgaagctgtc
     1861 cggcgacatg cccgcgctcc cccaaatcag attatttcgc gccgcgccgt atgtgccccc
     1921 gcagaaattc cgcaatatcc atgcggcgct ttccttccag ttgcacttcg cccacggaaa
     1981 gcgcgtcttt gccggcggct ataactagct ctttatcgct acgcaaaatc tctcccggtt
     2041 ttccgccaac atcgacaatg gacgcagaaa agattttcag atttcgctcg ccgagtttca
     2101 taaacgctcc cggccatgga ttgaacgcgc ggatttttct ctcaattatt tcggc
//
LOCUS       DASQUQ010000235         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_598566, whole genome shotgun sequence.
ACCESSION   DASQUQ010000235 DASQUQ010000000
VERSION     DASQUQ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598566"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VE486_06675"
     CDS             <1..784
                     /locus_tag="VE486_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZA38809.1"
                     /translation="RCGKNAEPLQVDWGKHIDHKAIDRQLATGKFDTVTLIHNETSTG
                     VMNSLGEICCILAKYPEVTLVVDTVSSFSGVKIDMDALGIDVMLTGAQKALALPPGFS
                     LFSVSQKAFERAEKIPNRGFYFDFLEFKKQQAQWMTPSTPSIAHIHALQSKLDEIFEE
                     GLEARFDRHVRTNALVHDWVCRAGFDFFAEEGFRSKTLTCVKNNRTIDVLKLASDLRS
                     QHHLVIDPGYGKIRGETFRLSNMGDETEETVAHLLACLDDVL"
     gene            901..1923
                     /locus_tag="VE486_06680"
     CDS             901..1923
                     /locus_tag="VE486_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZA38810.1"
                     /translation="MSAIEASGLTKVYRTYRKEGGLWGSIKGLAWRRYDETRAADHVS
                     FQIEEGEFVGFLGPNGAGKTTVLKMLSGLLNPTSGDARVLGFVPWERRNEMKRQFSLL
                     MGQKNALWWDLPAQESLELNRAIYGIDRARFKKVVDGLSELLEVQDKMNVMVRELSLG
                     ERMKMELISALIHEPRVLFLDEPTIGLDVVSQKRVREFLRIYNQEHRIVTLLTSHYMQ
                     DIEELCHRVLVIDHGNIFFDGPLAQIVDRFSGHKILSLTFEKEARRDFSAYGEVIEQT
                     AMSVQLKVPRAQVTETCRKLLEACDVSDINVQEVPVEEVIRQLFGERHENHRVAGLTS
                     AERVQA"
     gene            complement(1986..2891)
                     /locus_tag="VE486_06685"
     CDS             complement(1986..2891)
                     /locus_tag="VE486_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZA38811.1"
                     /translation="MPRILIAGCGYVGQATADLFHAAGWAVEGWTHSAASAVKLSAKP
                     YSVRAVDITDQARICEHAGVFDAIIHCASSGGGDADSYRQIYLNGAHNLLDRFGGSRM
                     LFTSSTSVYAQVDGSWVTEESETKPARETGRILLETEKLVLANDGIVARLAGIYGPGR
                     SALLSKFLAGQATIDPENDRFLNQVHRDDIAAALFLLLDRPSPAREIYNVVDDQPIVQ
                     SECYHWLAQRMNRAIPPLGRSASTLKRGASNKRVSNAKLRRLGWVPRYSTFAEAMEGS
                     ILPSVADNGQSPEPLRRLSPKAFAS"
BASE COUNT          695 a          854 c          850 g          676 t
ORIGIN      
        1 gcgctgcgga aaaaacgccg agccgctcca ggtcgactgg ggaaagcata tcgatcacaa
       61 agcaattgat cggcaattag cgaccggaaa attcgatacc gtcaccttga tccacaacga
      121 gacctcaacc ggtgtaatga actcgctcgg ggaaatctgt tgcatcctcg cgaagtatcc
      181 ggaagtcacg ttggtcgtcg ataccgtttc atctttttcc ggcgtgaaga tcgacatgga
      241 cgcgctcggt atcgacgtga tgctgaccgg cgcacaaaaa gcgctcgcgt tgccaccggg
      301 tttttcattg ttctccgtct cccagaaagc ttttgagcgc gcggaaaaaa ttccgaaccg
      361 tggcttttac tttgattttc tcgagttcaa aaagcagcag gcgcagtgga tgacacccag
      421 cactccgagt atcgcgcaca ttcacgctct gcaatccaag ctcgacgaaa tttttgaaga
      481 aggcctcgaa gcgcgctttg atcggcatgt ccgcacgaac gcgctggtgc acgattgggt
      541 ttgtcgagct ggcttcgatt tctttgccga ggaaggattc cgatcaaaaa cgctcacctg
      601 cgtgaaaaat aatcgaacca tcgacgtgct aaaacttgcg agtgatttgc ggtcccaaca
      661 tcatctcgtc atcgaccccg gctacggcaa aatccggggc gaaacctttc gcctctctaa
      721 tatgggcgac gaaaccgagg aaacggttgc gcacttgctc gcatgccttg acgacgttct
      781 ctagcgggtc ctgctcgccg cgctgaagct tggcaggtgg cacgtgtcaa gatacgacca
      841 ttttgctcac gccttgccac ggcgtagctt catgttgtag ctcgtcttca tctttgcttt
      901 atgtcagcga tcgaagcctc tggcctgacc aaagtttatc gtacctaccg caaggagggc
      961 ggtctctggg gttcgatcaa gggcctggcc tggcgacgct acgatgaaac gcgggcggcc
     1021 gatcacgtct ccttccaaat cgaggagggc gagtttgtcg ggtttctcgg tccgaacggc
     1081 gcgggcaaaa ccaccgtgct caagatgctg tccggcctgc tcaacccgac ctcgggggac
     1141 gcgcgagtgc tcggtttcgt tccgtgggag cggcgcaacg aaatgaaacg gcagttcagc
     1201 ctgctcatgg gacaaaagaa cgcgctctgg tgggacctgc ctgcgcaaga gtcactcgag
     1261 ctcaatcgcg ccatttatgg gatcgatcgc gcccggttca aaaaggttgt cgatggatta
     1321 tcggagcttc tcgaagtgca ggacaaaatg aatgtcatgg tgcgtgaact ctcgttaggc
     1381 gagcggatga agatggagct gatctccgcg ctgatccatg agccgcgcgt gctgtttctc
     1441 gacgaaccaa caattgggct cgatgtcgtc agccaaaaac gcgtccgcga gtttttgcgc
     1501 atttacaacc aggaacatcg gatcgttacc ctgctgacga gccattacat gcaggatatt
     1561 gaagaactgt gtcatcgcgt tctggtgatt gatcacggca acatcttctt cgacggtccg
     1621 ctcgcgcaga tcgttgatcg cttttcggga cacaagattc tcagcctcac tttcgaaaaa
     1681 gaggcaaggc gcgacttctc agcatatggc gaagtgatcg agcagacagc gatgtcggtg
     1741 caactgaaag ttccgcgcgc acaagtgacc gagacgtgcc ggaaactgct tgaagcatgc
     1801 gacgtcagcg acatcaacgt ccaggaagtg ccggtcgaag aagtaattcg ccaacttttc
     1861 ggtgaacggc atgagaatca ccgcgttgcc ggtctgacca gcgcagaaag agtgcaggct
     1921 taaatagacg cagaagaaac agcttccatc gcgataatcc tacacttcgg caggggcgat
     1981 tgacttcaac tcgcaaaagc tttcggactc aatcgccgga gcggttcggg ggactgcccg
     2041 ttatcggcaa cgctcggcaa aatgcttcct tccatcgctt cggcgaacgt cgaatatcgc
     2101 gggacccagc cgaggcgacg cagtttcgcg ttgctcacac gcttgttgct ggcgccgcgt
     2161 ttcaaagtcg atgccgatct tccaagtggc ggaatcgcgc gattcattct ctgagcgagc
     2221 caatgataac attcgctttg cacaattggt tgatcatcaa ccacattgta aatctcacgt
     2281 gctggtgacg gtcgatcgag caagagaaaa agtgcagccg cgatgtcgtc gcgatgcact
     2341 tgattaagaa accgatcatt ttctggatcg atagtcgctt gcccggccag aaatttgctc
     2401 agcagcgccg aacgtcccgg tccgtaaatg ccggcaaggc gcgcaacgat gccgtcattc
     2461 gcgagcacca atttttccgt ttcaagcagt attcgaccgg tctcgcgcgc cggtttggtc
     2521 tcgctttctt cggtgaccca cgaaccatcg acttgcgcat aaacgctcgt gctgctcgtg
     2581 aacagcatcc tcgatccacc aaatcgatcg agaagattgt gcgctccatt caagtagatt
     2641 tggcgatagc tatcggcgtc cccgccgccg gagctggcac agtggattat cgcgtcaaac
     2701 actccggcgt gctcacaaat ccgagcctgg tctgtgatat cgaccgcgcg aaccgaatat
     2761 ggcttggcgg aaagcttcac tgcggatgcc gcggaatgcg tccagccttc cactgcccaa
     2821 ccggcggcat gaaacaagtc cgcggtcgct tgtccaacat aaccacatcc agcgattaag
     2881 attcgcggca tgacgtgagg cgctgcgaat gcgagcggcg gctgtagcca cggcgctgcg
     2941 tcgccgtgcc gggtgcaacg cgcagggagc gcctcgacac agcgaggcgg ctacgctgtt
     3001 gcggccgcga tttcaatttc ttcaaagatg tttttctgaa tggcacggac ttgtttgaga
     3061 cgaatcagtt ccagg
//
LOCUS       DASQUQ010000236         5791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_530380, whole genome shotgun sequence.
ACCESSION   DASQUQ010000236 DASQUQ010000000
VERSION     DASQUQ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530380"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VE486_06690"
     CDS             <1..976
                     /locus_tag="VE486_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZA38812.1"
                     /translation="QIYDVDFSDVKGQGHVKRAIEVAVAGGHNVLMIGPPGSGKSMLA
                     KRIPTIIPPLSLEEAIETTKIHSIAGLLDSEKSFVSTRPFRDPHHTISDIGLLGGGTF
                     PNPGEVSIAHNGVLFLDELPEFKRSALEVMRQPLEDGKVTVSRAAGSVTFPSEFMLVA
                     AMNPCPCGYFGDLKRECRCGPVQVQRYRQRISGPLLDRIDLHIEVPAVEYRDVASERA
                     EESSTAIRGRIMQTRERQRERFQNDSKVTCNARMATRHLKQHCKLSQDSQDLIRVAMN
                     ELNLSARAYDRILKVSRTIADLDEKTDIAPEHVSEAIQYRTFDRTLWV"
     gene            1031..2509
                     /locus_tag="VE486_06695"
     CDS             1031..2509
                     /locus_tag="VE486_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZA38813.1"
                     /translation="MDPIVLIHGYSAESKQTTPAAIQKIYGSLPQALRDAYGNDGVIE
                     IDLSRYVTLEDGLTIDDISRAFDRVLKAEFASLLRGKFHVIIHSTGALVVRNWLRLFS
                     PKPSPIANLIYLAGANFGSGWGHIGKGQLAKWARLVFEGGAERGMQILDALELGAERT
                     LDLHLHFARPGNLMIADYGVHESVITGTQADVKWFAAPIRYAKEDGSDGVVRVSGANV
                     NFHYIRLGPTPEALEISWNEISTQTDRHLTREGKRKEYYEPKELSHPGTAERPRVPTG
                     IPFRCAHTGDDMGVVTGSRPRAQVMRMLRAALETTPETWPQLVAQFDAETGKTYERAI
                     KEEAPAWWKKWLDDPRAQYDHHAQLIFRIRDQHGFPVQHYDIFFDSVQRSIRGRPIQT
                     LFEDKHVNGKSPNVITFYLRTDKFVNKTEGWKSTLEEVKISSLEISAVEPETGDILYL
                     PCRIELDGAQLREWIQGHHTTIFDVELLRLPSPNVFRMVRFR"
     gene            2668..2985
                     /locus_tag="VE486_06700"
     CDS             2668..2985
                     /locus_tag="VE486_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38814.1"
                     /translation="MKPTILTTCNNSRRGSLDPAMPTRQMPLITNYNYHSATFESFRA
                     NCGRRSVRSFWNITGDYLKNEARQDFLGEAALFAVIVVTAFLPLISNAHALMEFVRAI
                     GNY"
     gene            3062..3766
                     /locus_tag="VE486_06705"
     CDS             3062..3766
                     /locus_tag="VE486_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38815.1"
                     /translation="MEETFEQFYRIEPTADISIKNRDGLVRLYGSLSNDMRVQAIKRA
                     YTRDRLKQIVVDVSVQPGSVSIETKFPPKPTWGLFDRSGTVEYTIVVPQTASISRLES
                     GNGEVLVEGMRGQTLHAQLERGRILARSCFSNAVFSLGHGTLTMTYGWWEPGKFSIQA
                     NVADGAVLAFLPGAAAFHLIAETPRGKIANDFVERARRDAEEASRVDTLIHGGGDAVI
                     KLQAKEGNIEVVRTNP"
     gene            3843..4328
                     /locus_tag="VE486_06710"
     CDS             3843..4328
                     /locus_tag="VE486_06710"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZA38816.1"
                     /translation="MPSFDIVSEVDKQELDNAINQARKELATRFDFKGTTAEILAEKD
                     KIILTAEDAARLRGLREIVIGKLSKRGVDLRNIEQADPEISSVGHARQELRIQQGLEG
                     NKAKEIIQAIKSQGFKVQSQLQDRQIRVTGKKKDDLQAVIQFVRGRDFGVATNFKNFR
                     D"
     gene            complement(4429..4740)
                     /locus_tag="VE486_06715"
     CDS             complement(4429..4740)
                     /locus_tag="VE486_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZA38817.1"
                     /translation="MNTHGIFQNNIGRKNSRALMKIQVQFYAQLRDLAGGDESGSCRI
                     DLSEGSTVRDLLDQIYAQRPALRAHDKSILIGAGVEFVDRNYKLKPGDQISIMPPVQG
                     G"
     gene            4729..5148
                     /locus_tag="VE486_06720"
     CDS             4729..5148
                     /locus_tag="VE486_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZA38818.1"
                     /translation="MRVHQEGLEVRTNGKGMYEITDDIQAKIDKCRLNNGTATVFVQH
                     TSCSLVIMENADPTARRDLEEFFDRLVSEDADYFTHGSEGSDDMPSHIRMVLTRTSET
                     IPIVDGKMQLGTWQGIFLFEHRRAPHRRKISITIIGE"
     gene            complement(5208..>5791)
                     /locus_tag="VE486_06725"
     CDS             complement(5208..>5791)
                     /locus_tag="VE486_06725"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZA38819.1"
                     /translation="LWIGLPEKENPRVATIYAAKSGKPPEPKQFYVDLATRGTMARKT
                     FTSPYGLHVVSAMNNPAIRAKSIVSFGGIGSAAALAKFYSMLANGEKCDGQTFFSERT
                     IASMTTTLADGVDRVFQIPTAFSAGFMKDSRKAARRIFGPSLNAFGHPGAGGSHAFAD
                     PENRIAFAYVMNQMEQSLLPNEKSLRLVHAIYG"
BASE COUNT         1395 a         1586 c         1573 g         1237 t
ORIGIN      
        1 tcaaatttac gatgttgatt tcagcgacgt gaagggccag ggccacgtaa aacgggccat
       61 cgaagtggcc gtcgccggcg gccacaacgt gctgatgatt ggtccccctg ggtcgggcaa
      121 atcgatgctg gccaaacgca ttccgactat tatcccgccg ctctcgctcg aagaagcgat
      181 tgagacgaca aaaattcata gcatcgcagg cttgctcgac tccgaaaaat ccttcgtgtc
      241 cacgcgacca tttcgcgatc cgcatcacac tattagcgat attgggctgc tcggcggcgg
      301 tacgtttccc aatcccggcg aagtcagcat cgcgcacaac ggggtcctct ttctcgatga
      361 gttgcctgag ttcaaacgat cggcgctgga agtaatgcgc cagccgctcg aggacgggaa
      421 ggtgaccgtt tcgcgcgcgg cgggaagcgt gacttttcct tctgagttca tgctggtcgc
      481 ggcgatgaat ccctgcccgt gtggatactt cggcgattta aagcgcgaat gccgttgtgg
      541 tcctgttcaa gtgcagcgct atcgccagcg aatctccggc ccattgctcg atcgaatcga
      601 tctgcacatc gaagtgccgg ccgtcgaata tcgagacgtc gccagcgagc gcgcggaaga
      661 aagttcgact gcgattcgcg gccggatcat gcaaacgcgt gaacgccagc gagagcgttt
      721 tcagaatgac tcgaaagtaa cctgcaacgc gcgcatggcc acgcgacatc tcaaacaaca
      781 ttgcaagctc agccaggatt cgcaggatct gattcgtgta gcgatgaatg aactaaatct
      841 gagcgcccgc gcttatgatc gcatcttgaa agtctcacgc acgattgccg atctggatga
      901 aaagaccgac atcgcacccg agcacgtcag cgaagcgatc caatatcgca ccttcgatcg
      961 caccttgtgg gtgtgagatc ggagttggaa ttggttgccc cgagactggt gagtccatag
     1021 acttacgctt atggatccca tcgtcctgat ccacggctac tccgctgaga gtaagcagac
     1081 tacgcccgca gcgatccaga aaatttacgg cagcttgccg caagctttgc gggatgccta
     1141 cggcaacgat ggcgtgatcg aaatcgacct tagccggtat gttactctgg aggatgggtt
     1201 aacaatcgac gatatttccc gcgctttcga ccgcgtcctg aaagcagagt tcgcatcgtt
     1261 attacgaggc aaattccacg tcatcattca cagcacaggc gcgcttgtcg ttcgaaactg
     1321 gctccgtttg tttagcccga aaccctcgcc gattgccaat ctcatctatc ttgctggcgc
     1381 aaattttggg agcggttggg gtcacatcgg aaaaggacag ttggcaaagt gggcgcgctt
     1441 ggtctttgaa ggcggtgcgg agcgcgggat gcaaatcctg gatgcgctcg agctgggagc
     1501 ggaacgcacg cttgacctcc atctgcactt tgcgcggccc ggaaatttga tgattgctga
     1561 ttacggagtg cacgaatccg tgatcaccgg gacacaggcg gatgtgaaat ggttcgccgc
     1621 gcctattcgt tatgcaaagg aagacggctc cgatggagtg gtgcgggttt ccggcgccaa
     1681 cgtgaatttt cattacatcc gactcggacc cacaccggaa gcgctggaga tttcatggaa
     1741 cgaaatcagc acgcagaccg accggcacct gacgcgcgaa ggcaagcgca aggaatacta
     1801 cgaaccgaag gaactcagtc atcctggcac ggccgaacgg ccgagagtgc cgacgggaat
     1861 tccgtttcgc tgcgcgcaca ctggtgatga tatgggggtc gtaaccggct caaggccgcg
     1921 cgcgcaggta atgcgaatgc tccgggccgc gcttgagaca acgccggaaa cctggccgca
     1981 attggtggcc caattcgatg ccgaaacagg aaaaacctac gagcgcgcga tcaaggaaga
     2041 agctccggca tggtggaaga aatggctgga tgatccgcgc gcccagtacg atcaccacgc
     2101 ccaattgatc tttcgcatcc gggatcaaca cggctttccc gtccaacact acgacatctt
     2161 ttttgactcg gtgcagcgat cgatacgcgg ccgtccaatt caaacgctct tcgaagacaa
     2221 gcacgtcaac ggcaagtcgc ccaacgtgat caccttttac ctccgcaccg acaagtttgt
     2281 aaataaaacc gaaggctgga agtccacgct cgaagaggta aaaatctcct cgctggaaat
     2341 ttctgccgtt gagccagaga ccggagacat tctctatttg ccgtgccgga tcgagttgga
     2401 cggcgcgcaa ctgagggaat ggatccaagg ccatcacacc acaattttcg acgtagagct
     2461 gctgcggctg ccgtcgccga atgttttccg catggtccgg ttccgctaga agcgctgaaa
     2521 ctgtcatgtc gagctccgaa agctttcggg gctgctgcga gattactgct tgctttcgtt
     2581 ggcgaacgaa attccgcaaa gaaacaaatt gtgaatccaa ttgggctgcg gggcgcatct
     2641 cacaggaaac aacaaacatc ggaaaccatg aaaccaacaa tattaacaac ctgtaacaac
     2701 agccgccgcg gctcgctcga tcccgcaatg cccacccgtc agatgccgct gattaccaac
     2761 tacaactatc actcggccac attcgagagt ttccgcgcaa attgtggacg gaggtcggtc
     2821 cgctcgtttt ggaacattac cggcgactac ctcaaaaacg aggcacgcca agattttctc
     2881 ggcgaagcgg cgctctttgc ggtcattgtc gtcacagcat ttcttccctt gatcagcaac
     2941 gcgcatgcgt tgatggaatt tgttcgtgcg attggcaact attaacagcc ggaacttttg
     3001 tctgttactt gctgcatcgt tcttgtcgct caacagctgc ggctccgaaa cgaaccccac
     3061 agtggaggag acgttcgagc aattctacag gattgaaccg accgcagata tttcaattaa
     3121 gaacagggac ggactcgttc gcctttacgg ctcgctttcg aacgacatgc gagtgcaggc
     3181 aatcaagaga gcctacacgc gggaccgatt gaaacagatt gtggtcgatg tctctgtgca
     3241 gccgggctcc gtctcgatcg aaaccaaatt tcccccgaaa ccaacctggg ggttgttcga
     3301 tcgctccggt acagttgaat acaccatcgt tgtcccgcaa accgccagca tatcccgatt
     3361 ggaatcaggc aacggcgaag ttctggtgga gggaatgcgt ggccaaacct tgcatgccca
     3421 attagagcgg ggacggatcc ttgctcgcag ttgtttcagt aacgcggtct tttcgctggg
     3481 ccacggcacg ctcaccatga cttacgggtg gtgggagccg ggcaaatttt ccatccaggc
     3541 aaacgtcgca gatggagctg tgttggcctt ccttccaggc gccgctgctt tccatctgat
     3601 tgccgaaacg ccccgcggaa agatcgcaaa tgatttcgtt gaaagagcca ggcgcgatgc
     3661 cgaagaggca tccagggtgg acacgttaat ccatggcggg ggcgacgccg tgattaaact
     3721 acaggcgaag gaaggcaata ttgaagtcgt gcgaactaat ccgtaaggcg aaatgatttg
     3781 actgcgaaag ctttcgcgag ttgatcaatc cgaaatccgc aatctaaaat cccgaatcgc
     3841 agatgccttc cttcgacatc gtctctgaag tggacaagca ggaactcgat aatgcgatca
     3901 atcaggcgcg caaagagctt gccacgcggt tcgattttaa ggggacaact gctgaaatcc
     3961 tcgccgagaa agacaaaata attctgaccg cggaagacgc cgcccgcctc cgcggattgc
     4021 gcgaaattgt catcggcaaa ctctccaaac gcggcgtcga tctccgaaac atcgagcaag
     4081 ctgatccgga aatctcatcc gtcggtcacg cccggcagga actaagaatc cagcagggcc
     4141 tcgagggaaa caaggcgaaa gaaattattc aggcgatcaa gtcgcagggt ttcaaggtgc
     4201 aaagccagtt gcaggatcgg caaattcgcg tcaccggaaa aaagaaagac gacttgcaag
     4261 ctgtgattca gttcgtgcgt ggccgcgact tcggtgtcgc caccaatttc aaaaatttcc
     4321 gcgactgagg tccagagatc ggacgtcaga ggtcagataa atgctcagca aacacgtcaa
     4381 ataccgccta agactttcga cttctgattt ctgacttctg atctccgatt atcccccctg
     4441 caccggcggc atgattgaaa tctggtcgcc cggcttcaat ttgtaattgc gatccacgaa
     4501 ttccacgccc gcgccaatca aaatgctttt gtcatgcgcg cgaagtgcag gccgctgcgc
     4561 gtaaatttgg tcgagtaaat cgcggacggt cgatccttcc gaaagatcga tccggcagct
     4621 gccggattca tcgcctccag ccagatcgcg caattgcgca taaaactgca cctgaatttt
     4681 catcaacgcg cgactattct ttctgccgat gttattctga aaaatcccat gcgtgttcac
     4741 caggaaggac tagaggttcg cactaacggc aaggggatgt acgaaatcac ggatgatatt
     4801 caggcgaaga tcgacaagtg cagactgaac aatgggacgg cgacggtttt tgttcaacac
     4861 acaagttgca gcctggtcat catggaaaat gcggacccga ccgcgcgacg cgatctggaa
     4921 gagttcttcg accggctggt gtcggaagat gctgactatt ttactcacgg ctcggaaggc
     4981 agcgatgaca tgccatcgca tattcgcatg gtcctgaccc gcacgagcga gacgatccca
     5041 atcgtagatg gaaaaatgca actcggaacg tggcagggaa tttttctgtt cgagcatcgt
     5101 cgcgcaccgc atcgacgaaa aatttccatc acgatcatcg gtgaataggt agggcgcgac
     5161 cgccgggcgc gccgacttct tcgacggacg gcccggtggt cccatcgcta cccataaatg
     5221 gcatggacaa gccgcaatga tttctcattg gggagaagtg attgctccat ttgattcatc
     5281 acgtacgcga acgcgattct attttccgga tcggcaaagg catggctgcc accggcgccg
     5341 ggatgtccaa aggcattcag tgacggcccg aaaattcttc gcgctgcctt ccgcgaatct
     5401 ttcataaagc ccgcggaaaa cgccgtcggt atttgaaaga cgcgatcaac gccatctgcc
     5461 agcgtcgttg tcatcgatgc gattgttctt tccgaaaaga aagtctggcc atcgcatttt
     5521 tcgccgttcg cgagcatgga atagaacttc gccaaggccg cggcgctccc gattcctccg
     5581 aatgaaacaa tcgatttggc gcggatcgcg ggattgttca tcgcgctaac cacatgcaat
     5641 ccgtagggag aggtgaacgt tttgcgcgcc atcgtgccgc gtgtagcaag atcgacgtaa
     5701 aattgtttcg gctcgggcgg tttgccgctt tttgccgcgt agatcgttgc cacgcgggga
     5761 ttctcttttt cgggcaaccc gatccaaaga t
//
LOCUS       DASQUQ010000237         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_57173, whole genome shotgun sequence.
ACCESSION   DASQUQ010000237 DASQUQ010000000
VERSION     DASQUQ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57173"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /gene="nrfD"
                     /locus_tag="VE486_06730"
     CDS             complement(<1..727)
                     /gene="nrfD"
                     /locus_tag="VE486_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZA38820.1"
                     /translation="MSAPDQSPLTSHESRSLSPLGYPTNLLAPGETYESVNKTISEIP
                     LSDRAPAAWRIGFVIAFLLLMMFLFAVSWLLIKGVGIWGVNIPVAWGFAIVNFVWWVG
                     IAHAGTFISAILLLLFQHWRTAINRFTEAMTLFAIACAGLMPLLHLGRPWVFYWLLPY
                     PDTMGLWPQFRSPLVWDVFAVGTYFTVSLLFWYLGLVPDLATVRDRAPKLWQKKIYGV
                     FALGWRNSAAHWQRHQIAYLLLAG"
     gene            complement(733..>3147)
                     /locus_tag="VE486_06735"
     CDS             complement(733..>3147)
                     /locus_tag="VE486_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZA38821.1"
                     /translation="AKSNAGATFRILTEPISSPTLLAQLADVLQEFPAARWCIWDPLN
                     RDSLTAGEGICDFSKAKIVVALDSDFLYTHPHALRYARDFANARRVITPDGARMNRLY
                     VAEPTPTITGSNADHRIAVAAHDILPFAQAIASNLGVGGGSRGKIDHQDWIEAVVGDL
                     KANRGASIFIVGETQPPEVHVVAAQINAALGNDGVTISPPLAISLPANRIALRDLVEE
                     MQRGAVELLIVLGGNPLYDAPIDFNFADAFNKVKLRIHHSVHFNETSRQSHWHVPAAH
                     FLESWSDTRAFDGSVSIVQPLIEPMYAGVSGHEILEALTGQSPRTSYEIVRGRWEAQN
                     SAPDFELKWRKALSDGVIAELETPSFQSWAINPSVSPLIEGEKNEVRGPKLEILFRPD
                     VSVRDGRYANNGWLQELPRQFSKLVWDNAALISPKLAQRENLENGDLVELTFSDRTLR
                     APVWIQPGQAENSITLPLGYGRNVVGNVGRNVGFNAYTLRTSDALWFGDGVTIRKTGH
                     THRFVSTQQHHAVNGRGILHDGTLDEFMSDPHFAQKPEQLPSRDNTLYYPEEYPYKGY
                     KWGMVVDLNVCIGCHACTIACQAENNIPIVGKQQVAVHREMHWIRVDTYYSGAGDAPK
                     FTHQPVPCMHCENAPCELVCPVAATAHDNEGLNLQIYNRCVGTRYCSNNCPYKVRRFN
                     FLEYNNRLSPTEKLVKNPDVTVRSRGVMEKCTYCIQRINAARIDSQLEQRAIRDGEII
                     PACAQVCPAEAIVFGDMNDLQSRVARLKHSLLSYWMLGELNTRPRTSYFAKLRNPSPA
                     LVRSDE"
BASE COUNT          737 a          882 c          868 g          660 t
ORIGIN      
        1 atccggccag cagcaaatac gcgatttgat gtcgttgcca atgtgccgcg gaattgcgcc
       61 agcccaacgc gaacacgccg tagattttct tctgccacag tttcggtgcg cgatcgcgca
      121 cggtcgcaag atcgggaacc aaaccgaggt accaaaacag caacgagact gtgaaatacg
      181 ttcccaccgc aaaaacatcc caaaccagcg ggctgcgaaa ttgcggccat aagcccatcg
      241 tatccggata tggaagcagc cagtaaaaaa cccagggccg cccaagatga agcagcggca
      301 tcaagcctgc gcaggcaatg gcgaacagcg tcatggcttc ggtaaagcga ttgatggcag
      361 tgcgccagtg ttgaaagagc aaaagcagga tcgcagaaat gaacgtgccc gcgtgagcga
      421 ttcccaccca ccaaacgaag ttcacaatcg caaagcccca tgcgaccgga atgttaactc
      481 cccaaatgcc cacccctttg ataaggagcc acgagactgc gaagaggaac atcatcaaca
      541 gtaggaaggc gatgacgaat ccgatccgcc aggccgccgg tgcacgatcg ctcagcggaa
      601 tctcgctgat cgttttgttg accgattcgt aggtttcgcc cggagcaaga agattggtcg
      661 gataacccag cggcgataaa gaacgtgatt cgtgagaagt gagaggtgat tggtcaggcg
      721 cgctcatact tctcactcat cacttctcac aagtgccgga ctcggattgc gcaacttggc
      781 gaaatagctt gtgcgcgggc gcgtattgag ttcgcccagc atccagtagc tgaggagaga
      841 atgcttcaag cgcgcgacgc ggctttggag atcgttcatg tccccgaaaa caatcgcctc
      901 ggccgggcag acctgcgcgc aggccgggat aatttcgccg tcgcggatgg cgcgctgctc
      961 cagttgagaa tcaatgcgcg ctgcgttgat gcgctggatg caatatgtgc atttctccat
     1021 tacgccgcgc gaacgcacgg taacatcggg attttttacc agcttctcgg ttgggctcag
     1081 ccgattgttg tattcgagaa aattaaagcg gcggactttg tacgggcagt tattggagca
     1141 ataacgggta ccaacgcagc gattataaat ttgcaggttc agcccctcat tgtcatgcgc
     1201 agtcgcggca accgggcaga caagctcgca cggcgcattc tcgcaatgca tgcacggcac
     1261 gggctgatgc gtaaatttcg gagcgtcgcc agcgcccgaa taataggtgt ccacgcggat
     1321 ccaatgcatt tcacgatgca cggccacctg ttgctttccg acaatcggaa tgttgttctc
     1381 tgcctggcag gcaatcgtgc aggcgtggca gccgatgcat acattaagat cgacaaccat
     1441 tccccacttg tagcctttgt agggatattc ctccggataa tagagcgtgt tatcgcgcga
     1501 aggcagttgc tcgggtttct gggcaaaatg cggatcagac atgaactcat caagcgtgcc
     1561 atcgtgcagg atcccccgac cgttcaccgc gtgatgctgt tgcgtgctaa caaacctgtg
     1621 cgtgtggcca gttttgcgga tcgttacgcc atcgccgaac caaagtgcat ccgaagttcg
     1681 cagggtataa gcattgaagc cgacattgcg ccccacgttg ccaacgacat ttctcccgta
     1741 accgagcggc aacgtaattg aattttccgc ctgtcccggc tgaatccaaa ccggcgctcg
     1801 caatgtgcga tcgctaaacg tcagctcgac aaggtcgccg ttctccaaat tttcgcgctg
     1861 agcgagtttt gggctgatga gagcagcgtt gtcccagacc agcttcgaaa attgtcgcgg
     1921 caattcctgc agccagccgt tgttggcgta gcgaccatcg cgcacactga catcggggcg
     1981 aaatagaatc tcgagtttgg gtcccctcac ctcgttcttc tccccttcaa taagcggaga
     2041 gacggatgga ttgatcgccc aagattgaaa cgacggcgtc tcgagttccg caattacccc
     2101 atcgcttaaa gcttttcgcc attttaactc gaaatccgga gcggaattct gcgcttccca
     2161 tcggccacgc acaatctcgt acgaagtgcg tggcgactgc cccgtgagtg cttcgagaat
     2221 ttcatgtccg gaaactcccg catacatcgg ctcgatcaac ggctgcacaa tcgataccga
     2281 accatcgaac gcgcgcgtat cgctccagga ttcgagaaaa tgcgccgcgg gcacatgcca
     2341 gtgcgactgt cgcgatgttt cgttgaaatg aacgctgtga tggatacgca gtttcacttt
     2401 attaaatgcg tccgcgaaat tgaaatcgat cggcgcgtcg taaagcggat tgccgccgag
     2461 gacgatgagc aactcgacag cgccgcgttg catttcctcg acgagatcgc gtagcgcgat
     2521 gcgatttgcc ggcagcgaga tcgcaagcgg tggcgaaatc gtcaccccat cgtttcccaa
     2581 tgctgcgtta atctgcgcgg ccaccacatg cacttccggc ggctgcgttt ccccgacaat
     2641 gaaaatgctt gctccccgat tggctttcag atcgccaacc actgcctcga tccaatcttg
     2701 atggtcaatc ttgccgcgag atccaccgcc gactccaaga ttcgacgcga ttgcctgcgc
     2761 gaacggcaga atgtcgtggg cggcaactgc gatacgatga tcggcgttcg aaccggtaat
     2821 ggttggtgtc ggttccgcaa cgtaaaggcg attcatgcgt gcgccatcgg gcgtgatcac
     2881 gcgccgcgca tttgcgaaat cgcgcgcgta tcgcagcgcg tgcggatgtg tgtaaagaaa
     2941 atcggagtcg agcgcgacta cgattttcgc cttggaaaaa tcgcaaatcc cctcgcctgc
     3001 ggtaagactg tcgcgattga ggggatccca gatacaccag cgcgcggcgg gaaattcttg
     3061 taatacatcg gccagttgag caagcaacgt aggcgagctg atcggctcgg taagaatgcg
     3121 gaaagtcgct ccggcattgg atttcgc
//
LOCUS       DASQUQ010000238         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_530618, whole genome shotgun sequence.
ACCESSION   DASQUQ010000238 DASQUQ010000000
VERSION     DASQUQ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530618"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..754
                     /locus_tag="VE486_06740"
     CDS             242..754
                     /locus_tag="VE486_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZA38822.1"
                     /translation="MKDREEVMDNVANHEDWKSCFSEVAPGLLLFARQWTRSPDDAED
                     IVQEAFVKFWRRNHQVNNRALLYATVRSIALDFIRRDRRRARREANVFAETESTIEPQ
                     FELEDETQAALAAAVDSLPHDQREVLVLKIWNELTFAEIAAALGISQNTAASRYRYAL
                     AGLKKSLQPQ"
     gene            751..1281
                     /locus_tag="VE486_06745"
     CDS             751..1281
                     /locus_tag="VE486_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38823.1"
                     /translation="MNDFSELENELKKLRPARPSPVLFARVEEAMASSESAKIVRPDR
                     FRINYVALGFGLAAAAVLFLLAKVGTDQRQEPAESIVQNSPPPETRTLLPGRRGDRIE
                     GSRSPNEFIPSGATQVVYNTRDEGLQFTDPSEQPLRRVRYQTQETWQWRNPATGASLR
                     VSYPSEEVVLIPVSGQ"
     gene            1469..2317
                     /locus_tag="VE486_06750"
     CDS             1469..2317
                     /locus_tag="VE486_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZA38824.1"
                     /translation="MIFLGVDTSRVPSVVSEQLGLAKGFGLVVDYVVPDSPASAAGIQ
                     QNDILKMLNDQILIEPSQLRKLLQTFSEGADVTLTILRKGQEQKITVKLTKKELPQRR
                     SWNPGGKHEWHWDFDEVGDLGEQMQDLKQQLQEQLGDAQRGVVRETVIKAREAAKRAR
                     EDARRAAREVRILSSDNGTLKATRIDLGKAQVVFSDDKGELKLETVAGKKVLTAKDPQ
                     GKLLFSGPIETREELDKVPAEIRQRYEQLQHKDLPKVAPRTDAEEDESGDADDDEDET
                     QSMEQV"
     gene            complement(2504..>3493)
                     /locus_tag="VE486_06755"
     CDS             complement(2504..>3493)
                     /locus_tag="VE486_06755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZA38825.1"
                     /translation="NHGKAAFDVKAIELELLQAGQVLDSKKLDGKAIERFADRGARAQ
                     AAGVFKQVAFQFCGDNLIAPSIKLAGPKLEKDQALLVTSQIFAFSGARDTLRVRVRGN
                     VAGRATEETGTLPIKSEFARNTYIFPLRGVSYVGWGASFHTGHRWAIPEEFALDIAKM
                     GGDGLSHKGDGTRFEDYYAYGTEVLASADGRVIGMANDQPEDTSAMQRPDESQEAYST
                     RLREEQAVRLAKGGILGNYVMIDHGKSEYSLSAHLQPGSVRVHVGDQVKAGDLIGKLG
                     SSGNSTEPHLHFHVCDSEDPLISAGIPVNFSNVTIQWADMPRPIQSGDIVIAK"
BASE COUNT          782 a         1016 c          975 g          720 t
ORIGIN      
        1 tctaaaaggc atctcgcatt atcgatattc ccctacggcg tttagataac gcaagatgcc
       61 tgggcatggg acgtggttgt agccgggatc ggtgatcccg gcggcagcgc acaagataag
      121 gcttcgaaga ccggcatcac cgatgccggc tacagcaatc gcgtcttctt ctaagaatcg
      181 ggaacggatc ccgaaaaaac ctcgccacta gacaaaatct gcatttggcg gcgtcttaga
      241 tatgaaagac cgtgaagagg tcatggacaa tgtcgctaac catgaggatt ggaagagttg
      301 tttttctgaa gtcgctcccg gcttgctgct tttcgcccgg caatggacgc gctcgcccga
      361 cgatgccgaa gacattgtcc aggaagcctt cgttaagttt tggcggcgca accaccaagt
      421 taacaaccgc gcgctgcttt acgccaccgt gcgttccatc gctctcgatt ttatccggcg
      481 cgaccgacgc cgcgctcgcc gcgaggccaa cgtttttgct gaaaccgagt caaccatcga
      541 gccgcagttc gaattggaag atgagacgca agcggccctc gctgcggcag tggattcttt
      601 gccgcacgac caacgggagg tgctggtttt gaaaatctgg aacgaactca cttttgccga
      661 gatcgcggcc gcgctgggca tctctcaaaa tactgccgca tcccgttatc gctacgcgct
      721 ggccggcctg aagaaaagtt tgcaaccgca atgaacgatt tctccgagct tgaaaatgag
      781 ttgaagaagc tgcgaccagc gcggccttcc cccgtgcttt ttgcacgcgt cgaagaagcg
      841 atggccagtt cggaaagcgc caaaatcgtt cggccggatc gttttcggat aaactacgtt
      901 gcgcttggtt tcggtctcgc ggccgcggcc gttttgtttt tgctcgcgaa agtcggcacg
      961 gaccaaaggc aggaaccagc cgaaagcatc gtgcagaatt cacctccgcc ggaaacaaga
     1021 actcttctgc ctggccgccg cggggaccga atcgaagggt cgagatcgcc gaatgaattt
     1081 attccttccg gtgcaacgca ggtcgtttac aacacgcgcg acgagggatt gcaattcact
     1141 gacccctcgg agcaaccgct gcggcgcgtt cgttatcaga cgcaggagac atggcagtgg
     1201 cgtaaccccg ccaccggcgc atcgctccgc gtctcttatc ccagcgagga agtcgtgctc
     1261 attcccgtct cgggccaatg atttaaccaa acaaaccatg aaaataaacc ttatcactac
     1321 catcgcagcc atcgccctct tgccggtcgc agggtttgct cagacgcaac cccagcctcc
     1381 aacgccgcct tcccagcccg gagccccccc ttcccagccc aatttgccac cgcggccggg
     1441 tcgcccggaa agagcgccca aagttccgat gatattcctc ggggtggaca catcgcgagt
     1501 cccaagcgtg gtcagcgaac aacttggcct ggccaaaggc tttgggttgg ttgtcgatta
     1561 cgtggtgccc gatagcccag cgtccgcggc cggcattcaa cagaacgaca ttctcaaaat
     1621 gttgaacgat cagattctga tcgagcccag ccaattgcga aagcttctcc agaccttctc
     1681 cgaaggtgcc gacgtgacgc tcacgattct gcgcaaaggt caggagcaaa agatcaccgt
     1741 caagctcact aaaaaagaac tgccgcagcg tcgttcatgg aatccgggag gcaaacacga
     1801 atggcactgg gatttcgacg aggtagggga tctcggcgag caaatgcaag atttgaagca
     1861 acaattgcaa gagcagttgg gcgacgcgca acgtggcgta gtccgtgaaa ctgtaataaa
     1921 agctcgcgag gcagcgaagc gggcgcgcga agatgcccgc cgtgccgcgc gggaggtccg
     1981 catcttaagc agcgataacg gtacgctaaa ggccaccagg atcgatcttg gcaaagcgca
     2041 ggtcgttttc tcggatgaca aaggcgagtt gaagctggaa actgtggctg ggaaaaaagt
     2101 gctcactgca aaggatccgc aggggaaact gctcttcagt ggtcccatcg aaacaagaga
     2161 ggagctcgac aaagtgccag ccgagattcg tcagcgttac gaacaattgc agcacaaaga
     2221 tttgccgaaa gttgcaccgc ggacggatgc cgaagaagat gaatccggcg acgcggacga
     2281 tgatgaagac gaaacgcaat cgatggaaca ggtatagatt tcattctgga gggaagccgt
     2341 ttgcttccca agttctttgg ggcagaggag agactccgcc gagcatctta acctggctcg
     2401 ggccagccac tcggcggagt tttcctttcc aagcaggcga tgtccgtttc gtttccggag
     2461 gtttgcggtt acgctgatca aacgttccgc gctgctgtac cgcttatttc gcgatcacga
     2521 tgtcgccgct ttggatggga cgcggcatgt ccgcccactg aatcgtgacg ttgctgaagt
     2581 tcaccggaat ccccgcgcta atcagcggat cttcgctatc gcacacgtga aaatgtaaat
     2641 gcggttcggt ggaatttccg gaggaaccga gcttgccgat cagatcaccc gctttcacct
     2701 gatctccaac gtgaacacgg acgctgcctg gttgcaggtg cgcggaaagc gaatactcgc
     2761 ttttgccgtg atcgatcatg acgtagttgc ctaagattcc ccctttggcg agccggacgg
     2821 cctgctcttc gcgcagtcga gtggagtatg cttcctgact ttcatctggc cgttgcatcg
     2881 ccgacgtatc ttcgggttga tcgtttgcca tgccgatgac gcgaccgtcg gcggacgcaa
     2941 ggacctcggt cccgtacgcg tagtaatcct cgaagcgagt gccgtcccct ttgtggctga
     3001 gtccgtctcc tcccatcttc gcgatgtcta aagcgaactc ttcgggaatt gcccagcggt
     3061 gcccggtatg gaatgacgcc ccccatccta catacgaaac gccgcgcagc ggaaagatgt
     3121 aagtgttcct ggcgaactcg gatttgatcg ggagcgtgcc ggtctcttcg gttgcgcgcc
     3181 cggccacatt cccacgaacg cgcacccgta gcgtatcgcg cgcgccactg aaagcgaaaa
     3241 tctgcgacgt gacgagcagc gcctgatctt tctccaactt tgggccggcc aatttgatgg
     3301 aaggcgcaat cagattgtcg ccgcagaatt gaaacgcgac ctgcttgaac acgcccgccg
     3361 cctgcgctct cgcgccgcga tcggcaaagc gctcaatcgc cttgccgtca agcttcttcg
     3421 agtcgaggac ctgcccggcc tgcaacagtt ccaactcgat cgctttcaca tcaaacgccg
     3481 ccttgccgtg gtt
//
LOCUS       DASQUQ010000239         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1229771, whole genome shotgun sequence.
ACCESSION   DASQUQ010000239 DASQUQ010000000
VERSION     DASQUQ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229771"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VE486_06760"
     CDS             <1..636
                     /locus_tag="VE486_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HZA38826.1"
                     /translation="FARDAHYSSRQQGATRAGLLWGAYHYGNATNPVRQADHFLQVVS
                     NAWAQADPASRPEGVLLVLDFEKNGHYPGGTMRVDQAITFVERIRDRTGKFPGVYSGE
                     YHLRQALNGPKVKLIDKAILANCWLWMANYGARPQATAPWSYWHLWQYTGDGKCRLPG
                     SGYPKSIANIRKAERNIFHGSRSALETFWQARAWRPGEKRLGEPARTVAER"
     gene            complement(1518..2042)
                     /locus_tag="VE486_06765"
     CDS             complement(1518..2042)
                     /locus_tag="VE486_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38827.1"
                     /translation="MRVAVLLATLLTTSASAVAGAISITSPDHAQTFVYGEMIWHQLY
                     LQRTGGELGARITFSNRPYADSNEPRRDEPFDFRFPGVQFDSTRRAFFASGRHGEPIS
                     VARFRGDSACGWIDLAPGAKIYLLKEDGRVTAILTATDYPRRGMRWIEMDDNFSLRNL
                     LVALFGELRARPDS"
     gene            2230..3816
                     /gene="pstC"
                     /locus_tag="VE486_06770"
     CDS             2230..3816
                     /gene="pstC"
                     /locus_tag="VE486_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZA38828.1"
                     /translation="MFRILIDVEAATTAPSFFRGKGLARRRRISTEAAIKAFFGSNAL
                     VAIVVLALITIFLFRDGFGFFGQNLANLRLYRQAGLEYVDIIRAQAEQQTALSRQLGD
                     IRLQEMRAGANQAALEKFDEFGNKFSDTGEQLNGLVSDATDQAQALRETMLQLGSHAG
                     ASVIESESQDEARAAAATPAGTEAEQMAALRSATDKFQQIADSMKAQISELLATPPAL
                     ATAKARKSFAKWKAEVEEYLKQLPAVVERLRAWDPNKPVAAYRAITSFLFGMSWTTAS
                     FWQDWYGIIPLFVGSLLVSIVALLIAVPLGVCGAIYVSEVASPTEKTLIKPYIEFISA
                     IPSVVLGFFGIAVLGETIRAISRASFLDWVPFFPISERLNVFTAGCLLALMAVPTIFT
                     LAEDALRNVPRGFKEASYALGANRLQTIVRVLVPASLSGIVSAILLGLGRVIGETMVV
                     LLCAGNRIAIPDFTQGLGAFFQPVHTMTGIIAQEMGEVVRGSIHYRALFMVGLVLFVI
                     TLAINYGAQKLVMRYRMSIG"
     gene            3868..>4176
                     /locus_tag="VE486_06775"
     CDS             3868..>4176
                     /locus_tag="VE486_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38829.1"
                     /translation="MFWTFRLATYLVLACATYIFLDIAIKGSRTVFTNHAPFINVKFL
                     TEPPQTLYVFEYQGKKMELSDREFRKWQETHKEPVDATSIAYSAGGIWPCIVGTALLV
                     I"
BASE COUNT          898 a         1203 c         1153 g          922 t
ORIGIN      
        1 ttcgcgcgcg atgcgcacta ttcctcgaga cagcaggggg cgacgcgggc gggattgctc
       61 tggggcgcct accattatgg taatgcgacc aaccctgtca ggcaggccga tcattttctg
      121 caggtcgttt ccaatgcctg ggcccaggcc gatccagcat cgcgaccgga aggcgtcctg
      181 ctcgtgctcg atttcgaaaa aaatggtcac tatcccggcg gcacaatgcg cgttgatcag
      241 gcgatcacat tcgtcgaacg gattcgcgac cgaactggca agttcccggg cgtttactcc
      301 ggcgaatatc atctgcgaca ggcgctaaac ggcccaaagg taaaactgat agataaagcg
      361 atattggcga attgctggtt gtggatggcg aactacggtg ctcggccgca ggccaccgcg
      421 ccatggagtt actggcatct ttggcagtac accggtgatg gcaaatgccg tctgccgggt
      481 tctggttatc cgaagagcat agccaacatc cgcaaggcag aacgcaatat tttccatggc
      541 agccgaagtg cgctggaaac gttctggcag gcacgcgcct ggcgtcccgg cgaaaaacgt
      601 ctgggcgaac cagctcggac ggtggctgaa cgctaagccc attatccgct gaggataccc
      661 ccgaacgctt tcggggctac atgtagcggg cgtatccgtc ccatttgtct cattcaggcg
      721 gaatcgaaat gcgtcacgac tccattctaa ctcggcgcgc gctggagaaa ttcgttcagc
      781 acatcggcag cggcgaagat tggccaggct gagatggcca ttattatcgc aaaaagcagc
      841 acttcgacgg caaattccca acgcttttcg ttcgtgaaat aacttcgggt taatccgcga
      901 aatgacctca gaatcagtga tcgccgatat ggcgtccgtt tgcacaccgt tctctgcact
      961 gtgattacac tcatagttgg gcgttcaata acccgccccg cgcgacacgt catgtgacga
     1021 atcgtaaaga ttgcgtgacg aaaatgtcac gggtgcttgc cgaatcatct cctagcaaca
     1081 atttcaggcg accctcatga tttgggaatg aatcgaatta ttgctgcctt ggcgatcgcc
     1141 ggccttctct tcatttcggc gtgcgaattc ttcggtccag gtgccgagga gcaggttcgg
     1201 cagcaacccc ggaacgccgt ttatacgtct gctgacccaa gttcgctcga caatccaact
     1261 gttacgccca ggtccgaaaa agtgccaggg gagtaggcac cgtctgttct agatccgacc
     1321 ttaaattctc ctatgagcgc gctcctgctg cgcgcgccag tactgctaga aacactacca
     1381 gcgagagcgc tgacacgagc aagcatgcga tcagacccaa aaccaagaat ccgatcttca
     1441 cttgcggaag atgccccttt cctgctttgc tgcggagtgg ggttaactgc ggcctaggcg
     1501 gagatctcag gcgagtttta gctgtcgggc cgcgcacgga gctctccgaa cagcgcaact
     1561 agaagattcc ggagcgaaaa attgtcgtcc atctcgatcc aacgcattcc cctgcgcgga
     1621 taatcggttg cggtcaaaat ggcggtaacg cgaccgtcct ctttgagcag atagatcttc
     1681 gcgcctggcg ccaaatcaat ccagccacag gccgaatcgc cgcgaaaccg cgcgaccgaa
     1741 atcggttcac cgtgccggcc actcgcaaag aatgcgcgtc gcgtggaatc gaattggaca
     1801 cctggaaagc gaaagtcgaa cggttcatcc cttcgcggct cgttgctgtc ggcataaggc
     1861 cgattactaa acgtgatccg cgcccccagt tcgccgcctg tgcgttggag gtagagctgg
     1921 tgccaaatca tctcgccgta gacaaacgtc tgcgcgtgat cggggctggt gatgctgatc
     1981 gcgcctgcaa ccgccgaagc gcttgtcgtg agaagagttg ccagcaaaac cgcgactcgc
     2041 attctatgaa tctaaggcga gacccgcgat cgtccagcgt ctgcctcgat catctgctcc
     2101 cagatcagat cacgattcgc aagtcatttc cttcctccac cgagaccttc aaacgggttg
     2161 taaacctgga tcgcgtaata agcgcaatgt caccaaaacg taacattaat tcctttgccg
     2221 gctggaagca tgttccgcat cctcatcgat gtggaagctg ccacgaccgc cccttctttc
     2281 tttcgcggaa agggacttgc ccgccgccgt cggataagca cggaagcagc aatcaaggct
     2341 tttttcggca gcaacgcgct cgtcgcaatc gtggtgcttg cgttgattac gatttttctt
     2401 tttcgcgatg gcttcggatt tttcggccaa aacctggcga atttgcggct gtatcggcag
     2461 gcgggtcttg aatacgtgga catcattcgg gcgcaagccg aacagcagac ggctctctcg
     2521 cgtcaactcg gcgacatccg gcttcaagaa atgcgagcgg gagccaacca ggcagcgctg
     2581 gaaaaattcg atgagtttgg aaacaaattc agcgacaccg gcgaacagtt aaacggattg
     2641 gtatcggacg cgaccgatca agcgcaggcg ctgcgtgaaa caatgctaca gcttggcagc
     2701 catgctggtg cttctgtgat cgagagcgaa tcgcaggatg aagctcgcgc cgccgccgcc
     2761 acgccggctg gcaccgaagc ggagcaaatg gctgcgcttc gttctgcaac agacaagttc
     2821 cagcaaattg ccgattcgat gaaggcgcag atcagtgaac tgctggcgac gcctccggca
     2881 ttggccactg ccaaagcgcg caaatccttc gcgaaatgga aagccgaagt tgaagagtat
     2941 ctcaaacagc ttcccgcagt tgtcgaaaga cttcgcgcgt gggatccaaa caaaccagtg
     3001 gccgcctatc gcgcgatcac ttctttcctc tttgggatga gctggaccac ggcaagtttt
     3061 tggcaggatt ggtacggcat cattccgttg tttgtgggtt cgctgctggt ctcgatcgtc
     3121 gcgctgctaa ttgctgttcc cctcggcgtt tgtggagcga tctacgtgag cgaggttgcc
     3181 tcgccgaccg agaagaccct gatcaaacca tacattgaat tcatctcggc gatcccgtcc
     3241 gtcgtgctgg gtttcttcgg gattgccgtc ctcggagaaa cgatccgcgc tatctcacgt
     3301 gcctcgttct tggactgggt gccgttcttt cccatcagcg agcgactcaa cgtgttcacc
     3361 gcgggttgtt tgctcgcgct gatggctgta cccacgattt ttaccctcgc cgaagacgca
     3421 cttcgcaacg tgcctcgcgg tttcaaggaa gcctcctacg ccttgggcgc gaatcgactt
     3481 cagacgatcg tacgcgtgct cgtgccggcg tcgttgtcgg gaattgtatc cgcgattttg
     3541 ctgggactcg gccgcgtcat cggagaaacc atggtggtgc tgctctgcgc cggaaaccgg
     3601 atcgccattc ctgatttcac ccagggactc ggcgcatttt tccagccggt gcacacgatg
     3661 accggaatca tcgcgcagga aatgggcgag gtggttcggg gcagcattca ctaccgcgcg
     3721 ctttttatgg tgggccttgt gctgttcgtg atcacgctcg ccatcaacta cggcgcgcaa
     3781 aagctcgtga tgcgctatcg catgagcatc ggataaatga tcgccccgcc ttccaatcgc
     3841 cgccgcagcc gtttcttcca agggttcatg ttttggacct ttcgacttgc tacctacctc
     3901 gtgctggcct gcgccaccta catctttctc gatatcgcaa tcaagggaag ccgcacggtg
     3961 ttcaccaatc acgcgccgtt tatcaatgtg aagtttctga ccgagccgcc gcagacgctc
     4021 tacgtgttcg agtatcaggg caaaaaaatg gagctgagcg atcgcgaatt ccggaaatgg
     4081 caggaaacgc acaaggaacc cgttgatgcc accagcatcg cttattctgc cggcggaatc
     4141 tggccttgta tcgttggcac ggcgctgctc gtcatc
//
LOCUS       DASQUQ010000240         4236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_57613, whole genome shotgun sequence.
ACCESSION   DASQUQ010000240 DASQUQ010000000
VERSION     DASQUQ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57613"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..746
                     /locus_tag="VE486_06780"
     CDS             192..746
                     /locus_tag="VE486_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38830.1"
                     /translation="MRKITPLLVVGLCCLAFYPRSAEAVVMSAPKPKFAYKDASGKKQ
                     SVEIVDKYYPKKIVKPFAKADTNIDPKLRRAATIAEERARAHSLSKCWRFVKEALVAA
                     GVVKSRPQTPLAKQAGQELVKNHGFKKLPVSDPYEAPVGSVLVYGAKRAAGHVEIRTE
                     NGFVSDFRSKTPSRRPLLGVFAKS"
     gene            833..1561
                     /locus_tag="VE486_06785"
     CDS             833..1561
                     /locus_tag="VE486_06785"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38831.1"
                     /translation="MQPIRLAMWSGPRNISTAMMRAWGNRADTRVIDEPFYAYYLKAT
                     GKKHPGAEEVIAAGETDWQKVVARLTGSVPSGKSIFFQKQMAHHLLPEVDREWLAGVT
                     NCFLIRDPREVIVSYIKKQEDPTLEDLGFLQQEEIFDWVRARTGAAPPVVDARDMLEN
                     PRRTLGLLCDAVGVEFSDSMLSWSPGLRATDGIWAKYWYGEVARSTSFQPYRPRHAKV
                     PAHLQEIHDRCREGYQRLYECRLH"
     gene            1711..2616
                     /locus_tag="VE486_06790"
     CDS             1711..2616
                     /locus_tag="VE486_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZA38832.1"
                     /translation="MLQRFNEKNRDLIVSINGQLIHRDKAGISPFDSVVQGGDAVWEG
                     LRLYNGRIFKLYEHLDRLHRSAVALSFIEIPPRDKIIEEIKRTLGANKMRDGVHIRLT
                     LTRGVKITSGMDPRLNQSGPTLIVLAEHKAAVYAKTGLTLITSKVRRPPPEVLDARIH
                     HANLLNSILAKIEANNAGADDALMLDTRGFVAETNATHVFIVRNGDLATSRVVACPEG
                     ITRATVIEICAAEKIRCVETDLSPVDVYRAGEMFCTGTMGELAAVTKIDNRVIGDGKV
                     GAITKRLSDLYAQRTATEGVQVVDV"
     gene            complement(2643..3257)
                     /gene="msrA"
                     /locus_tag="VE486_06795"
     CDS             complement(2643..3257)
                     /gene="msrA"
                     /locus_tag="VE486_06795"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZA38833.1"
                     /translation="MTTKLCVYFLFAAMGLTVGLARAAEPTIPNTKTAILAGGCFWCI
                     QPVFDKAKGVLKTVVGYSGGTEPNPTYELVTSEKTHYRESIEITYDPAKISYEQLLDI
                     YWRQIDPTQADGQFTDIGPSYRAAIFYGNDEEKKIAEASKAKLAGSGKFDKPIVTEIL
                     PAMKFYPAEAHHQKYYEKNAAHFEAFERGSGRVSFQKKKWDEGR"
     gene            3342..3734
                     /locus_tag="VE486_06800"
     CDS             3342..3734
                     /locus_tag="VE486_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38834.1"
                     /translation="MANLSDNSAGNRSAEQAFQILRFGFTVAPILAGLDKFFHVLADW
                     DKYLSPTANNVLAGHGHQFMLVVGIIEIIAGIGVFLKPRVFGYIVAAWLLGIIINLLS
                     IPGYYDIALRDLGLMLGALALASLSPRS"
     gene            3801..>4236
                     /locus_tag="VE486_06805"
     CDS             3801..>4236
                     /locus_tag="VE486_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38835.1"
                     /translation="MPAGEVETAHRPESFRGSGLIKLDSVIDPLDLDKIFGRNAPLQV
                     DLGCGDGSFICELAERMPERNFLGIERLIGRIRSAERKAATVCNVHLLRMESSYAVRY
                     LLSAGSVETFYLLFPDPWPKRRHWRRRIVTAEFLRAISEALAE"
BASE COUNT          919 a         1105 c         1202 g         1010 t
ORIGIN      
        1 tcggaatgac aaaaattgcg cctagaagca cttgctcttt cgagcaagaa tttttgcacc
       61 ggagtcgttc cggcttgtgc gcgcgtaaat gactcgagtc caacgattta atgtcagtcg
      121 caaaaattca tgattttgtt aaacaaatcg cttgcagaag cagggaaact gatcgatatg
      181 aggcgctctt tatgcgaaaa atcacccccc ttctcgttgt cggtctgtgt tgcctcgcgt
      241 tttatccgcg ttcagccgag gcggtggtca tgtccgcacc aaaaccgaag tttgcttaca
      301 aggatgcttc cggcaaaaag cagtcggttg agatcgtgga caaatattat ccgaaaaaga
      361 tcgttaagcc atttgcgaag gccgatacaa acatcgaccc caaactccgt cgggcggcca
      421 ccattgccga ggaacgcgcg cgcgcgcact cgctttccaa gtgctggcgt tttgtcaaag
      481 aagcgctggt tgctgccggt gtcgtgaagt cccggccgca aacgcccttg gctaagcaag
      541 caggccagga gctggtgaaa aatcacggct tcaagaaact cccggtatcc gatccgtatg
      601 aagcgccggt cggctcggtg ttggtgtatg gcgccaagcg cgcggccggt cacgttgaga
      661 ttcgcactga gaacggcttt gtgagcgatt tccgctccaa aacgccgtcc cgccggccgc
      721 ttttgggtgt cttcgcaaag agttagctcg cgcgggtcgc cgatcctgag caggccgaaa
      781 gaatcaattc tggctggcaa attatccagc ggcgctggag tctttgctga tcatgcagcc
      841 aattcgcctc gcgatgtggt caggcccgcg aaacatctcg accgcgatga tgcgcgcatg
      901 gggtaatcgc gccgacacca gagtcatcga cgagccgttt tatgcgtact atttgaaagc
      961 gaccggcaag aagcaccccg gcgcagagga agtgattgca gccggcgaaa ccgattggca
     1021 gaaagtcgtt gcgcgcctga ccgggtcggt tccgagcggc aagtcgattt tttttcagaa
     1081 gcagatggcg caccatctct tgccggaggt agatcgcgaa tggcttgccg gtgtgacgaa
     1141 ctgttttctc attcgcgatc cgcgcgaggt aattgtctct tatatcaaga agcaagagga
     1201 cccgacgctc gaagaccttg ggtttctaca acaggaggag atttttgatt gggtgcgcgc
     1261 ccgcaccggt gcggcgccgc cggtggtgga tgcgcgcgat atgctggaaa atccgcgaag
     1321 aactttggga ttattgtgcg atgcggtcgg ggtcgagttt agcgactcga tgctttcctg
     1381 gtcgccgggg ctacgcgcaa ccgacggaat ctgggcgaaa tattggtacg gcgaagtggc
     1441 gcgatcgaca tcgtttcagc cctatcgtcc gaggcacgcc aaggtgcccg cacatttaca
     1501 agaaattcac gatcgctgtc gtgaaggcta ccagcgacta tatgaatgcc gattgcacta
     1561 gcatctcttg ttgtcattct gagcgaagcg aagaagctct gattatttcc tggccgaaac
     1621 gccagcgcgg agagtcagag atgtttcgct ccgctcaaca tgagagagcc atatctatca
     1681 gcgaaatccg cgtagcccgc ggttgaacct atgcttcaga gatttaacga gaaaaaccgc
     1741 gatctgatcg tcagtatcaa tgggcaactg atccatcgcg acaaagccgg gattagtcct
     1801 tttgattcgg tggtgcaggg tggagatgcc gtctgggaag ggttgcggct ctataacgga
     1861 cggattttca agctctacga acaccttgat cgacttcatc gttccgcagt tgcgctgtcg
     1921 ttcatcgaaa ttccgcctcg cgacaagatc atcgaggaaa tcaagcgcac gctcggcgca
     1981 aacaagatgc gagacggcgt tcacattcgc ctcacgctca cgcgcggcgt gaaaatcaca
     2041 tcgggaatgg atccgcggct gaaccaatca gggccaacgc tgatcgtgct cgccgaacat
     2101 aaggcggccg tttacgcgaa gaccggactg actctgatca cgagcaaggt tcgccgtcca
     2161 ccgcccgagg ttctcgacgc gcgaattcat cacgcgaatc tgctgaactc gattctcgcc
     2221 aaaatcgaag caaacaacgc cggagcggac gatgccctga tgctggacac gcgtggcttt
     2281 gtcgcggaaa caaatgccac gcatgtcttt atcgtgcgca atggcgatct tgcgacgagc
     2341 cgcgtcgtgg cgtgtcctga aggaattact cgcgcgacgg tgatcgagat ttgtgctgcc
     2401 gaaaaaattc gttgtgtcga aaccgatttg tcgcctgtcg atgtttaccg cgccggtgaa
     2461 atgttttgca ccggcacgat gggcgagctg gccgccgtca caaagatcga caatcgcgtc
     2521 atcggcgacg gcaaagtcgg ggcgatcaca aaacgcctga gcgatcttta cgctcaacgc
     2581 accgcgactg agggcgtgca agttgttgat gtgtagcggc cccgaaagct ttcggggtcg
     2641 ctttatcgcc cttcgtccca cttcttcttt tgaaaagaaa ctcgtcccga tcctctttcg
     2701 aatgcctcga aatgcgccgc gtttttctcg tagtattttt ggtgatgcgc ctcggccgga
     2761 tagaatttca tcgccggcaa aatttcggtc acgatcggtt tatcgaattt gcccgagccc
     2821 gcgagctttg cttttgaagc ttcggcgatc ttcttttcct catcgttgcc ataaaagatc
     2881 gcagcgcgat aacttggtcc gatatcggtg aactggccat ccgcctgcgt cggatcgatt
     2941 tgtcgccaat aaatgtcgag caactgctcg taagagattt tcgccggatc gtaagtgatt
     3001 tcgatggatt cgcggtagtg cgttttctcc gaggtaacga gttcgtaggt tggattcggt
     3061 tctgttccgc cagaataacc aacgacggtt ttgagcacgc cttttgcctt gtcgaacacg
     3121 ggctgaatgc accaaaaaca gccgccagcg aggatcgcgg tcttcgtgtt tggaatagtt
     3181 ggctcggcgg cgcgggcgag accgacagtt aatcccatcg ccgcgaacaa aaaataaacg
     3241 cagagtttcg tcgtcattgt ccatcttaga cagcgagagc tgaactttgt ttggattggt
     3301 tttgacttgt taatgggtgg gtcgcctgca ataacagctt tatggcaaac ttaagtgaca
     3361 attcagcagg aaatcgatct gctgagcaag cgtttcagat tctccgcttc ggcttcactg
     3421 tggcgccgat tctggctggc ttagataaat tcttccatgt gttggctgac tgggataaat
     3481 acctgtcgcc gaccgcgaac aatgtcttgg cgggccacgg tcatcaattc atgctcgtgg
     3541 tcggtatcat cgagatcatt gctgggattg gagtgtttct taagccgcga gttttcggtt
     3601 acattgtggc tgcgtggctg ctggggatta tcattaacct tctcagcatt cctggttact
     3661 acgacatcgc cctgcgcgat ttgggtctga tgctaggcgc gttggcgctc gccagcttga
     3721 gcccgagatc ttgatctgat gctgtttctc ggcttcggcg catcgagtag caaagcgaca
     3781 gtttaatttt tctcctcagc gtgcctgcgg gcgaggttga aacggctcac cgacccgaaa
     3841 gctttcgggg ttccggactg atcaaactcg attcggtgat cgatccgctc gatttggaca
     3901 agatcttcgg acgcaacgcg ccgttgcagg tcgatctcgg ctgcggcgac ggctcgttca
     3961 tttgcgaact ggcagagcgc atgccagaga gaaattttct cggaatcgag cggttaatcg
     4021 gtcgcatccg cagtgccgag cgaaaggccg ccacggtttg caatgtgcat ctgctgcgca
     4081 tggaaagttc gtatgcggtg cgctatctgt tgtcggctgg gtcggtggaa acattttatc
     4141 tcttgttccc cgatccatgg ccaaaacggc gtcattggcg gcgcaggatc gtgaccgcag
     4201 aattcctgcg cgcgattagc gaggcactgg ctgaaa
//
LOCUS       DASQUQ010000241         6538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_876170, whole genome shotgun sequence.
ACCESSION   DASQUQ010000241 DASQUQ010000000
VERSION     DASQUQ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876170"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1488
                     /locus_tag="VE486_06810"
     CDS             85..1488
                     /locus_tag="VE486_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZA38836.1"
                     /translation="MSLVFKTIQTEGIAELSYLLGDDDEGIGAIFDPRADVDVYVEMA
                     REAGLAITHIFETHIHADLVSGSRELRARLESAKIFVSHEGGAFYGFEHEKVKDGDCF
                     TFGEVLVTARHTPGHTPEHLSYLLAEADHPDTPWGILTGDSLFVSSAGRPDLLGADHT
                     NELAEKQFRTLRDFYLNLPDHVMIYPNHGAGSPCGADIGDRLSSTIGYERRFNKFLQF
                     DDVKKFTDYALTSAPPVPKYYPVMKKINAKGPEILGNLPSVPALPPNAFKEAIDKKAG
                     VLVDTRTMLAFGGGHIPGAFNIAGSPILSIWAGWMLDPNEPILLVLENENDLVEIVRL
                     FIRTGYTKFAGYLVGGMKAWDAAGFPLEKIGQMSVHELNERKSSLQILDVRSPGEWKR
                     GHVPGARHIFLPELTKRVGELDRAKPTAVYCGSGYRASIATSILKSEGFNELWNVPGS
                     WEAWKKARFPVEGADGK"
     gene            1485..2288
                     /locus_tag="VE486_06815"
     CDS             1485..2288
                     /locus_tag="VE486_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38837.1"
                     /translation="MNGAEATIPRRSFGQTMRQDVWWTHPLLVFLGLSIFIVYSTWAA
                     FKGSHYFSGNYVSPFYSPELFGDSPHSWFGPKPAWWPGWLIFSPALLVLWAPGGFRVT
                     CYYYRGAYYKAFWADPPACTVGEPRKGYWGERSFPLIMQNVHRYFLYLALVFILILTY
                     DVWKALWFVDPTSGKTSFGIGVGTIVLAINVVLLGGYTFGCHSLRHLAGGFLDQFSKS
                     PTCYRAYACVTCFNRRHMLWAWISLFWVAFTDLYVRLCSMGIWTDWRIL"
     gene            2374..4185
                     /locus_tag="VE486_06820"
     CDS             2374..4185
                     /locus_tag="VE486_06820"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HZA38838.1"
                     /translation="MAEYETFEHDVLVIGAGGAGLRAAIEASAAGVRVGLVCKSLLGK
                     AHTVMAEGGIAAALANVDERDNWKVHFADTMRGGQYVNNWRMAELHAKEAPDRVRELE
                     AWGAVFDRTKDGRILQRHFGGHKYPRLAHVGDRTGLEMIRTLQDHGVHQGIDVYMEHT
                     ILSLLKDGGRVVGAFGYERERGRFKIFYAKAVVLATGGIGRAYKITSNSWEYTGDGHA
                     LAYEAGAELIDMEFVQFHPTGMVWPPSVMGILVTEGVRGEGGTLTNKEGKRFMFDAIP
                     ENYRAQTADNEEEGWRYCQGDKNARRPPELLTRDHVSRCIVREIKEGRGSPHGGVFLD
                     ISWIKQKMPNAAEHIKRKLPSMYHQFKQLADIDITEQPMEVGPTTHYVMGGIRVDPDT
                     QMSRVPGLFAAGECAAGINGANRLGGNSLSDLLVFGKRAGEFAAKFAKENSPGQTDNG
                     RIELVAREALAPFDRSNGENPYQVQKDLQDTMQDLVGIVRNESEMREALEEINGFKKR
                     AEKAAVTGNREYNPGWHTALDLENLLIVSEAITRAALERKESRGAQFREDYPDKDNAF
                     GKVNTIVSKAADGSMQVRLEPLPEMPEYLRQVIEEMK"
     gene            4376..5128
                     /locus_tag="VE486_06825"
     CDS             4376..5128
                     /locus_tag="VE486_06825"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HZA38839.1"
                     /translation="MKTATFKIWRGDAKSGEFRDYTTEVAEGMVVLDAVHDIQRIQAP
                     DLACRWNCKAGKCGSCSAEVNGMPKLMCMTRLSELPLERPVTVEPMKAFPVVKDLITD
                     VSWNFRVKKKIKKFKPRPPDAPDGTWRMQQEDIDRVQEFRKCIECFLCQDVCHVLRDH
                     QLHDKFIGPRFLIHVAALEMHPLDTENRIDELRNAQGIGYCNITKCCTKVCPESIEIT
                     DNGIIPLKERVVDNFYDPFGWIWRWLKKKLNR"
     gene            5220..5720
                     /locus_tag="VE486_06830"
     CDS             5220..5720
                     /locus_tag="VE486_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38840.1"
                     /translation="MSELKPLSKDAIPAALKKAERYRLLNEPGEAESICLDVLKSDPE
                     NQEAITTLLLALTDRFTKGYGVGDTQIKQLLGRIRSEYERTYYSGIFAERRAKAKLAQ
                     HTPGCRFQAYELFREAMDWFEKAEGIRPSGNDDALLRWNTCARIIERNRLVPREEEEP
                     IEFPLE"
     gene            5801..>6538
                     /locus_tag="VE486_06835"
     CDS             5801..>6538
                     /locus_tag="VE486_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZA38841.1"
                     /translation="MVKTKDEFSRFEHEGWERVADKYDSVWSPLTRQFIPYLADAAQV
                     LPGMSVLDVACGPGYVSDAIRKLGAIPMGIDFSERMVAIAKKMFPDIRFAQGDAQNLP
                     RDDASFDRVLINFGLLHLSHPEQACAEASHVLRSRGKFGFTVWAPPSQSPGAKIVNDA
                     IEAHADLNVQLPEGPPRYLYDQKEECRAVLEKAGFDGRTMIYETRSVEWHLPTAHYFF
                     EAERDAGVRTAGLLARQSPEKLNAIRTA"
BASE COUNT         1529 a         1635 c         1887 g         1487 t
ORIGIN      
        1 cgcgagacgc gtgcgctacc cgaaacgctg cgtgcaatcc ggatcttgtt gaagttagcg
       61 tccgcttcga cgtagagtca gtcaatgtcg ctcgttttca aaaccattca gactgaaggg
      121 atcgcggagc tttcttacct gctcggcgac gacgatgagg ggatcggagc gatttttgat
      181 ccgcgggcgg atgtcgatgt ttacgtggag atggcgcgcg aagcgggcct tgccatcacg
      241 catatcttcg agacgcacat tcatgccgat ttggtgagtg gttcgcgcga acttcgcgcc
      301 cggcttgaat cagcgaagat tttcgttagc cacgagggcg gcgcgttcta cggattcgag
      361 cacgagaaag taaaagacgg cgactgtttc acgtttggtg aagtcctggt caccgcgcga
      421 catacccctg gccacacacc ggagcacttg tcgtatctgc tcgccgaagc agatcatcct
      481 gatacgccct ggggcatact cacgggcgat tccttgttcg tctcatccgc aggtcgaccg
      541 gatttgttgg gcgcggatca caccaacgag ctcgccgaaa aacaatttcg cacgttgcgc
      601 gatttttatc tcaacttgcc cgatcacgtg atgatttatc cgaatcacgg cgccggttcg
      661 ccgtgcggcg cggacattgg ggaccgcttg agtagcacaa tcggttacga gagaaggttc
      721 aataaatttc ttcagttcga tgacgtgaag aagttcaccg actacgcgct cacgagtgcg
      781 ccaccggtgc cgaagtacta tccggtgatg aaaaagataa acgcgaaagg cccggaaata
      841 cttggcaatc tacctagcgt tccggcactt ccgccgaatg cgtttaagga ggcgatcgac
      901 aaaaaagcgg gcgtgctcgt ggatacccgg accatgctcg cgtttggcgg cggtcatatt
      961 ccgggcgcgt tcaacattgc tggctcgccc atcctatcga tttgggcagg ttggatgctg
     1021 gatccaaacg agccgattct tttagtgctc gaaaatgaaa acgatctcgt agaaatcgtg
     1081 cggctgttta ttcggacggg ctacacaaag ttcgcgggat acctggtggg tggaatgaaa
     1141 gcgtgggacg cggctggttt tccactcgaa aaaatcgggc agatgagcgt gcatgagctt
     1201 aacgagcgca aatcatcgct tcagattctc gatgtccgtt cgccgggtga atggaagcgc
     1261 ggacatgttc ccggcgcacg ccacattttc ctaccggagc tgaccaagcg ggtgggcgaa
     1321 cttgatcggg caaagcctac tgcggtttat tgcggaagcg gttatcgcgc gagcatcgcg
     1381 acgagcattc taaaatcaga aggctttaat gagctttgga acgttcccgg cagctgggaa
     1441 gcttggaaaa aggcgcggtt ccccgtggaa ggagccgacg gcaaatgaac ggagcagagg
     1501 caaccatccc gcgacgctcg tttgggcaaa cgatgcgcca ggatgtctgg tggacgcatc
     1561 cgctgctggt ctttctcggt ttgtcgatct tcatcgtgta ctccacgtgg gcggcgttta
     1621 agggaagcca ttatttttct ggcaattacg tctcgccgtt ttattcgccg gaactttttg
     1681 gtgactcgcc acacagctgg tttggaccca aacccgcgtg gtggcccgga tggctaattt
     1741 tttctcccgc tttgttggtt ctttgggcgc ccggtggatt tcgtgtcacg tgttattatt
     1801 accggggcgc gtactacaaa gcgttttggg cggacccccc ggcgtgcaca gttggcgaac
     1861 ctcgcaaagg ctactggggc gaacgttctt ttccgctgat tatgcaaaac gttcaccgct
     1921 attttcttta tctggcgctc gtttttattc tgatcctcac ctatgacgtg tggaaagcgc
     1981 tttggtttgt cgatccaaca agcggaaaga cctcctttgg catcggcgtt gggaccatcg
     2041 ttcttgcaat taacgtcgtt ctgctcggcg gctatacgtt tggttgtcat tccttgcgac
     2101 atctggctgg tggatttctc gatcaatttt caaaatcacc aacttgctat cgcgcttacg
     2161 cctgcgtgac ctgtttcaac cggcgccaca tgctctgggc ctggataagt ttgttctggg
     2221 ttgctttcac tgatctttat gtacggcttt gctcgatggg gatctggaca gattggcgaa
     2281 tcttatagcg caagtgattc gctaaagtgt tacaaagtta aagcggtttc ttttgtaacg
     2341 ttgtgactgt tttagcgctt taactctcaa tccatggccg agtacgaaac cttcgagcac
     2401 gacgttctgg tgatcggcgc tggaggtgcg ggactccgcg ccgcgattga agcttcggct
     2461 gccggagttc gcgtcgggct tgtttgcaaa tctctgttag gcaaagcgca taccgtaatg
     2521 gccgagggcg gaattgccgc cgcgctagcc aatgtcgatg agcgcgacaa ctggaaggtg
     2581 cactttgccg ataccatgcg tggcggacaa tatgtgaaca attggcgcat ggccgaactg
     2641 catgcgaagg aagcgcccga tcgcgtgcgg gagctggaag catggggcgc cgtatttgat
     2701 cgcacgaaag atggtcgaat tctgcaaaga cattttggcg gccacaaata tccgcgcctt
     2761 gctcacgtgg gcgatcgcac tggtctcgaa atgattcgca ccctgcaaga tcacggtgtt
     2821 caccagggga tcgatgttta catggagcac acgattcttt cgttattgaa agatggcggg
     2881 cgcgtggtgg gcgcctttgg ttacgagcgc gagcgcgggc gtttcaaaat tttttacgcc
     2941 aaagcagttg tgctggcgac cggcgggatt ggtcgcgcct acaaaatcac cagcaacagc
     3001 tgggaataca cgggggatgg acacgcgctc gcgtacgagg ccggtgccga actcatcgat
     3061 atggagtttg ttcaatttca tcccaccggc atggtctggc caccgagtgt aatggggatt
     3121 ttggtgaccg aaggcgtgcg cggtgaaggc ggaaccctga ctaacaaaga gggcaagcgt
     3181 ttcatgttcg atgcgatccc ggaaaattat cgcgcgcaga ccgccgacaa cgaagaagaa
     3241 ggctggcgct attgccaggg agacaaaaat gcgcggcgtc cacccgagct gctgacccgc
     3301 gatcatgtct cgcgctgcat cgtgcgcgag attaaggaag ggcgcgggag tccgcacggg
     3361 ggcgtatttc tcgatatctc ctggatcaaa caaaaaatgc ccaacgcggc agagcacatt
     3421 aaaaggaagc tgccgagcat gtatcatcaa ttcaaacaac ttgccgatat cgatatcact
     3481 gagcagccga tggaggtcgg gccaacaacg cattacgtca tgggagggat acgcgtcgat
     3541 cccgacacac aaatgtcgcg tgtgcccgga ttgtttgccg caggtgagtg cgcagccgga
     3601 atcaacggcg cgaaccgcct cggcgggaat tcgctctccg atcttttggt gttcggcaaa
     3661 cgcgcaggcg aattcgccgc gaaattcgca aaagagaatt cgcccggcca gaccgacaac
     3721 ggcaggatcg aattggtcgc gcgtgaagcg cttgctccat tcgaccgaag taatggcgaa
     3781 aatccttatc aggtgcaaaa ggatttgcag gacaccatgc aagacttggt tgggatcgta
     3841 cgtaacgaaa gcgaaatgcg cgaagcattg gaggagatca atggttttaa aaaacgtgct
     3901 gagaaagccg cagtcacagg taatcgtgaa tacaatccgg gttggcacac tgcgctcgac
     3961 ttagaaaatc tgctcatcgt ctcggaggca atcacgcgcg cggcgctcga aagaaaggaa
     4021 agtcgtggcg cacaattccg ggaagattac ccggataagg acaacgcttt cggcaaagtg
     4081 aacacgatcg tgtcgaaagc tgcggatggg tcgatgcaag ttcgattaga accgctcccg
     4141 gaaatgccgg agtatttgag acaggttatt gaggaaatga aatgagcgct cgcggtcaac
     4201 gattgggtag cgcacgcgtc tcgcgtgctg gttgcggcgt cgcgccgcaa tggacttttc
     4261 gagaaccttc cgtcttgcat ccctcggcat atccaaagaa aagttcgcga tcgcgaggac
     4321 gtgctcgcca gcacgcgaga cgcgtacgct acccgaaaac atcgcttcac tcgttatgaa
     4381 aacagccacc ttcaaaatct ggcgaggcga tgcgaagagc ggcgaattcc gcgattacac
     4441 cacggaagtg gccgaaggga tggtcgtgct tgatgcggtg catgatatcc agcgcataca
     4501 agcaccggac cttgcatgtc gatggaattg taaggcagga aagtgcggat cgtgttcggc
     4561 ggaagtgaac gggatgccga agctcatgtg catgacacgc ctgagcgaac tgccgttgga
     4621 gagaccggtg accgtcgaac caatgaaagc gtttccggtg gtcaaagatt tgatcaccga
     4681 cgtttcctgg aattttcggg taaagaaaaa gatcaagaaa tttaagccgc gaccgccgga
     4741 tgcgccggat ggaacctggc gcatgcaaca ggaagacatt gatcgtgtgc aggaatttcg
     4801 gaaatgcatt gagtgttttc tttgccagga tgtttgccat gtgctgcgcg atcaccagct
     4861 acacgataaa tttatcggac cgcgttttct cattcatgtg gcggcgctcg aaatgcatcc
     4921 gctcgacacc gaaaatcgca tcgatgaact gcgaaatgcg caaggaatcg gctactgcaa
     4981 tatcacgaag tgttgcacga aagtttgccc tgagagcatt gaaatcaccg ataacggaat
     5041 cattccgctg aaagaacggg tggtggataa tttctatgat ccgtttggat ggatttggag
     5101 gtggctcaag aagaaattaa accgttaaaa aatgttaaag gttacaaaat tgatgtgatt
     5161 gttaacagat ccagttttaa cgatgtaacc tttttagccc ctttaactgc ttcaatgcga
     5221 tgtccgagct aaagccactt tccaaagacg ccattcccgc cgcgctgaaa aaagccgagc
     5281 gctaccgtct tctcaacgaa ccgggcgaag cggaaagtat ttgcctcgat gtcctcaaaa
     5341 gcgatccgga aaaccaggaa gcaattacta cgctgctgct cgcgctaacg gatcgtttta
     5401 cgaagggcta cggagtgggc gacacacaga tcaagcaatt gctcgggcgc attcgcagcg
     5461 agtatgagcg cacctattac tccggaatct tcgccgagcg tcgcgccaag gccaaactgg
     5521 cccaacacac cccgggctgc cgatttcagg cctacgagct ttttcgcgaa gcgatggatt
     5581 ggtttgagaa ggcagaaggg attcgtccgt ccggaaacga tgatgcattg ctgcgttgga
     5641 atacttgcgc ccgaattatc gagcgaaaca ggcttgttcc gcgtgaggaa gaagagccga
     5701 tcgaatttcc gctggagtag cgaacgatag gctgccagcc tgtctcggcc gacaagcatc
     5761 ttgcttgtcg aagaattttc ccgacagata tgtcatcacg atggtaaaga ccaaagacga
     5821 atttagtcgc ttcgaacacg aaggctggga gcgcgtggcg gacaaatacg attctgtgtg
     5881 gtcgccactg acgcggcaat ttattcctta tttagccgat gctgcccagg ttttaccggg
     5941 gatgtccgtg ctggatgtcg cgtgcggacc cggttacgtg tccgacgcaa tcagaaagtt
     6001 gggcgcaatt ccaatgggca tcgacttctc cgaaaggatg gtggcaatcg cgaagaaaat
     6061 gtttcccgac atccgatttg ctcaaggcga tgctcaaaat ttacctcgtg atgatgcgag
     6121 ctttgatcgg gtgctgataa attttggtct actgcatctt tctcaccccg aacaggcgtg
     6181 cgcggaagcg tcccatgttt tgagatcgcg tggcaaattc ggtttcactg tctgggcgcc
     6241 accatcgcag agcccagggg ctaagattgt gaacgacgcg attgaagccc acgcagatct
     6301 gaatgtgcag ttaccagaag gtcctccgcg ttatttatac gaccaaaaag aggaatgccg
     6361 ggcggtgctg gaaaaagccg ggttcgatgg gcgcacgatg atttatgaga cgcgctcggt
     6421 tgaatggcat ttgccaactg cgcattattt cttcgaggcc gagcgtgatg cgggggttcg
     6481 caccgctgga ctcctggcgc gccaatcgcc agaaaaactg aacgcgattc gcacggcg
//
LOCUS       DASQUQ010000242         4123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_790649, whole genome shotgun sequence.
ACCESSION   DASQUQ010000242 DASQUQ010000000
VERSION     DASQUQ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790649"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..630
                     /locus_tag="VE486_06840"
     CDS             175..630
                     /locus_tag="VE486_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38842.1"
                     /translation="MSVLSMLACIDLELVKAIAALIGAGAAVVAILVYRSNSRLQRAK
                     WLADLYEKFYERSDLKRVREVLDCEAGDSPSVSALVRDEPAEFSDYLNFFEFVAVLGK
                     SGQLRKQEIEDLFRYYLDCLENCRAVRNYIAEKGYEQLDRLLRERAMEK"
     gene            627..1028
                     /locus_tag="VE486_06845"
     CDS             627..1028
                     /locus_tag="VE486_06845"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HZA38843.1"
                     /translation="MKDYLFAYGTLANENVPEEIASTVEQLKYVGDGFIFGRLYDLGE
                     YPGAVLDGDRRNKVFGKIFELPDDREVLECLDAYEAFDPQQPSKSLFVRRRTTISRPK
                     QPPLMGWMYEYRGNVGALPLIENGQYSKAST"
     gene            1031..1705
                     /locus_tag="VE486_06850"
     CDS             1031..1705
                     /locus_tag="VE486_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZA38844.1"
                     /translation="MTMESLPEIKARAEAAVPGSKIDIVSNPGPAQQSSLLIDNEHAS
                     AVARFLRIDPALRLDFCSNATGVDWLDRIVKKTAKVKTIVDGEEKEVDQTTEEKIPGY
                     LEAVYHLYSMTHKHGPVVIRMRTSDRAEGARLPSLTPIWRSAELQEREIFDLYGIRFE
                     GHPDLRRILMWDEFVDHPMRKDYREPDDYEYEPTPHDEVLERAKQHYTARPQVDGAEN
                     ITAQQP"
     gene            1702..2868
                     /locus_tag="VE486_06855"
     CDS             1702..2868
                     /locus_tag="VE486_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-quinone oxidoreductase"
                     /protein_id="HZA38845.1"
                     /translation="MTATIQTLEPVERPPTTMGSRLEGELLEVSMGPQHPSTHGVFRM
                     NVALEGEIVRKLKPVFGYLHRNHEKIGENTSYLGSMPYTDRLDYFCSMTNNWAYALSV
                     EKLAGIEVPERAQYLRVILAELTRLQNHASLLGFLLNDMGAWGTPLMYAFREREKILD
                     LFESLSGSRMMCDYMRFGGCRCDTPQGWMAYARKLVDAFPQFIDELEQLVVGNEVVIA
                     RTQNVGKLSAELAINAGITGPMLRACGVNYDLRKVDGYGFYPRFKFRIPLGEHGDTYD
                     RLMMRVLEMRESVGILKQAFEQIPTGPIMNPKVKIRAFRPPVGEAYGRIEAPKGELGF
                     YLISDGGTNPYRYRVRPPSFINLTVLEDLCLGHTVADVMVILGSVDIVMGEVDR"
     gene            3067..3672
                     /locus_tag="VE486_06860"
     CDS             3067..3672
                     /locus_tag="VE486_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZA38846.1"
                     /translation="MIGTGILKGMAVTARNFVGSYFEKDRLTTVQYPEERNPLPENYR
                     NFPFLPYDGDDPHAGLRCVACKICEKECPPQCIYIIKSEDKKPDYMGKPQFYPAVFDI
                     DISVCMSCQICVEVCPFEAIKMDKVFELSKRERFDALLLRKSALAKSNEYYHKICPTD
                     ATEVDAKLAAVAEAAAKKKAAAAAGPAGGQPPAPTQLSSST"
BASE COUNT          969 a         1078 c         1178 g          898 t
ORIGIN      
        1 attcatggaa ctgcagcgca agatcgacag tcaaaaacta acgggcgaca acaaggccga
       61 gcatctcgat ccggcggaac cgagcgaatt cccggtgcca tcatttggcg agcacgattt
      121 ggagccgccg gcgaatccgg acgtctttca tccgccgcgg atagagagac aggcgtgagc
      181 gtcctttcaa tgttggcgtg tatcgatctg gaattggtta aagccattgc cgcactcatc
      241 ggagcaggcg cagcggtcgt agctattctt gtttaccgtt ccaactcccg cctgcagcgc
      301 gccaaatggc tggcagattt gtacgagaaa ttttacgagc gcagcgatct caaacgggtg
      361 cgggaagttc tcgactgtga agcgggcgat tcaccgagcg taagcgcttt agtgcgtgat
      421 gaacccgcgg aatttagcga ttatctcaat ttcttcgaat tcgtcgctgt tcttggcaaa
      481 tccggtcaat tgcgcaaaca ggaaattgaa gacctgttcc gctactattt ggattgtttg
      541 gagaattgcc gtgcggtgcg aaactatatc gctgaaaagg gctacgagca gttagacaga
      601 ctgctgcgtg aacgcgctat ggagaaatga aagactactt gtttgcttac ggaaccttgg
      661 cgaacgaaaa cgtgccggag gaaattgcca gcacggtcga gcaactgaag tatgtcggcg
      721 acggcttcat cttcggtcgc ttgtacgatc tgggcgaata tcccggtgcg gttttggacg
      781 gcgacaggcg caacaaggtt ttcggaaaaa tcttcgagtt gcctgacgat cgagaggtgc
      841 tggagtgtct ggacgcctac gaagcttttg atccgcagca gccgagcaaa agcctctttg
      901 tacggcggcg gacgacgatc agccgcccaa agcaaccgcc gctgatgggc tggatgtacg
      961 agtaccgcgg gaacgtcggc gcgctgccgc tgatcgagaa tggccagtat tccaaagcat
     1021 ctacttgagc atgacgatgg aatccttgcc ggagatcaaa gcacgcgccg aagcggcggt
     1081 gccgggctct aagatcgaca tcgtttcgaa tccgggtccc gcccagcaat cgtcgctact
     1141 gatcgacaat gaacatgcat cggcggtggc gcgtttcttg cgcatcgatc cagcgctgcg
     1201 actcgatttc tgttcgaacg cgaccggcgt cgattggctt gatcggatcg tgaaaaaaac
     1261 cgcaaaagtg aaaacgattg tcgatggtga ggaaaaggaa gtcgatcaaa ccacggaaga
     1321 aaaaattccc ggttatctcg aagcggtata tcacctttac tcgatgacac acaaacacgg
     1381 tccggtggtg attcgaatgc gcacgtcaga ccgcgcagag ggcgcccgtt tgccctcgct
     1441 tactcccatc tggcgcagcg ccgaacttca ggagcgagag attttcgatc tgtacggcat
     1501 ccggttcgaa ggccatccgg atctgcggcg cattctgatg tgggacgagt ttgtcgatca
     1561 tccgatgcgc aaagattatc gcgaacccga cgattacgaa tacgagccga cgccgcacga
     1621 tgaagtgctg gaaagagcga aacagcatta caccgcacgg ccacaggtgg atggcgccga
     1681 aaatatcaca gcgcaacaac catgaccgcg accattcaaa cgctggaacc agttgagcgt
     1741 ccgcctacga caatgggctc gcgcctcgag ggcgagctgc tcgaggtctc gatgggaccg
     1801 cagcacccat cgactcatgg tgtgtttcgc atgaacgtcg ctctcgaagg cgagatcgtg
     1861 cgtaagctca agccggtctt cggatacttg catcgcaacc acgaaaagat cggcgagaac
     1921 accagttatc tcggctcgat gccgtacacc gaccggctcg attacttttg ctcaatgacg
     1981 aacaactggg cgtatgccct cagcgtggag aagctcgccg gcatcgaagt tcctgagcgt
     2041 gcccagtatc tgcgcgtcat tctggcggag ctgacgcgtc tgcaaaatca cgcgtcgctg
     2101 ctcggatttc tgctcaacga tatgggcgca tggggcacgc cgctgatgta tgcgttccgc
     2161 gagcgcgaaa agattctcga tctcttcgaa tcgctctccg gctcacgcat gatgtgcgac
     2221 tacatgcggt ttggcggctg ccggtgcgac acgccccaag gctggatggc atatgccagg
     2281 aagcttgtcg atgccttccc ccaatttatt gacgagttgg agcaattggt agtcgggaac
     2341 gaagttgtta tcgcgcgcac gcagaatgtc ggcaaacttt cagctgagtt ggcgatcaat
     2401 gcaggcatca ccggcccaat gctgcgtgca tgcggggtca attacgatct tcggaaggtg
     2461 gatggttacg gattctatcc gcgtttcaaa ttccgcatcc cgcttggcga acacggcgac
     2521 acctacgacc ggttgatgat gcgcgtgctc gaaatgcgcg agagcgtggg aattttgaag
     2581 caagcgttcg aacaaattcc cactggtcca atcatgaatc caaaagtgaa gatccgtgcc
     2641 tttcgtccgc ccgtgggcga agcgtacgga cgaatcgaag cgcccaaggg agaactcggt
     2701 ttctatttga tcagcgatgg cggaacgaac ccatatcgtt atcgggtgcg tccgccgagt
     2761 ttcatcaacc tgacagttct cgaggatctc tgtctaggcc acaccgtcgc cgacgtgatg
     2821 gtaattctcg gcagcgtcga tatcgtgatg ggagaagtgg atcggtgagg caaacatgaa
     2881 gcagaaaagt attctggtcc tagcctcgct aatgttggcc tatggcgtcg ctgtagcgtg
     2941 gtcaggtcat gtagtttggg aggatggagt tgctgaaatt tttggagccc aagctcgggc
     3001 tgtaggagct ctgagtattc ttttctcggt ggcacttttt tttacatatt ttcgaaggcg
     3061 caaatcatga tcggcacagg aatattgaag ggaatggcgg tcaccgcgcg gaacttcgtc
     3121 ggaagttatt tcgagaagga ccgattgacc acagtgcaat atccggaaga gcgcaatccg
     3181 ctgccggaga actaccgcaa ttttccgttc ctcccttatg acggcgatga tccccacgcg
     3241 ggcctgcgct gcgtggcttg caagatttgc gagaaggaat gcccgccaca gtgcatctat
     3301 atcatcaaga gcgaagacaa gaagcccgat tacatgggca agccgcagtt ttatcctgcg
     3361 gtgttcgaca tcgatatctc agtctgtatg agctgccaaa tttgcgtcga ggtctgtcca
     3421 tttgaagcga tcaagatgga taaggttttc gagctgagta agcgcgagcg atttgatgcg
     3481 ttgctcctgc gcaagagtgc tctggcgaag tcgaacgaat attatcataa aatctgtcca
     3541 accgatgcga ctgaggtcga tgctaagctc gccgccgtag cagaggctgc ggcgaaaaag
     3601 aaagcagctg cggccgctgg ccctgctgga ggtcagccgc ccgcaccaac acaattatcg
     3661 tcctcgactt aatcgtgagt tactcatgaa cttgctgaaa cttgactgct taaagcagtc
     3721 aagtttgcgg agccaatccc gaagttcgcg gcaagctgcc gcgaacaaca ggctggcagc
     3781 ctgtgctccc ctgaaaagaa ctgcagcgtc gcggctcatt tgcaatcgcc aattgaaaaa
     3841 tctacaatcg aaaatcgaca ggccagcggt ggcgaaatct attctcgcgt tggattatca
     3901 ccgcgcttgc gatgcaggcg gtcgtaagtg tcaaacacgc ctttagggga gcgcgcagat
     3961 atgccacaat atatctgcta cgggaatgcg gtcgtccgcg agcagatgat cctcacggcg
     4021 gcggtttggt ccccatgaca acgcgcacct cgtgaggcag cgcgtcgtca cgcaacgtca
     4081 cgattttatc ggccgccggg actccatcca attccatcag agt
//
LOCUS       DASQUQ010000243         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_791652, whole genome shotgun sequence.
ACCESSION   DASQUQ010000243 DASQUQ010000000
VERSION     DASQUQ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791652"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VE486_06865"
     CDS             complement(<1..371)
                     /locus_tag="VE486_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZA38847.1"
                     /translation="MIKAIFFDAVGTLMHLTRTVGDDYALVGSEVGLTLDARQLDRAF
                     YKAWNEMPRRTAIDGPRPHDDKGWWRDLVNLVLDQVSPSLSEFDRDNFFEIAYEHFAE
                     AGPWELYPEVVDVLENLHPRF"
     gene            529..1638
                     /locus_tag="VE486_06870"
     CDS             529..1638
                     /locus_tag="VE486_06870"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38848.1"
                     /translation="MLPTEHNDPINEKILAISEDKIDGFVREPFEEIARRSGVSVDVV
                     MARIAAMLRAGTIRRVRQTLLATNLADGALVAWKVPEDKIDNAFDWMFQRDPFSGHVV
                     LRSTDTVTAGSDYKLWTTVKVPHGFSLQQHCELLAKKVGAERFRVMPAKGIFTLGVGH
                     VRRKTIEPGSRSEEPAKMIPVQVVNLSEQEWNILLALKREFVPEEIRPTPWAARAEEA
                     GVSLDEFYRVAGQLNARKIIGRFSTFLEHVKPSASGVRVTRFNALFHWAVPPGREIEA
                     GGEVGRHHILTHCYWREAGPEFKNVNIMAVAHGTDKQLLLEHKGAIDRHLQASGIPVS
                     YTNVFWGGRSEIKPSEISPHVYREWLAEQVKELKR"
     gene            complement(1676..2344)
                     /locus_tag="VE486_06875"
     CDS             complement(1676..2344)
                     /locus_tag="VE486_06875"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZA38849.1"
                     /translation="MSPAEILEQLTADEMQAALGNAVIGREIIVLDQTGSTNDAILQI
                     TTPNSDEGLVLFAEHQTAGRGQRGNRWESAAAKGLWFSILLRPGIEINRSPLLTAWAA
                     KTISGAIEDEFSLKTTIKLPNDVQIDGRKVGGVLVEMRAQEKARHLAIVGIGINVNQA
                     LEDFPRELRGPAISLAMALGRQVDRQKFAIALLRNLDRSYKENFGGSSSPLLDVRSGG
                     QAAN"
     gene            complement(2331..3179)
                     /gene="nadC"
                     /locus_tag="VE486_06880"
     CDS             complement(2331..3179)
                     /gene="nadC"
                     /locus_tag="VE486_06880"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZA38850.1"
                     /translation="MKPDHYDPIAAALKEDIGQGDITTDFFVPATLHATGRIIAREKA
                     IVAGTGAAGEVFRQVDPSIDTQILRRDGDEVVGGDVIIEVRGLARSILKAERVALNFL
                     QRLCGIATLTRQFVDAVKNHPAKILDTRKTTPGLRSLEKAAVLAGGGGNHRFGLYDMV
                     LVKDNHLMILDGLSGFADRIKQIREERENIRIEVEADDLDQVRAFVEIQGIDVILLDN
                     MAPAQIREAVALKTDNIKFEASGGITLKNVNRIAATGVDYISVGSLTHAARAIDIGLE
                     MTHVPG"
BASE COUNT          849 a          986 c         1016 g          831 t
ORIGIN      
        1 tgaaagcgcg gatgcaggtt ctccaaaacg tctacgactt cgggatacag ttcccaggga
       61 ccggcctcgg cgaaatgttc gtaggcgatt tcgaaaaaat tatcgcgatc aaattcgctc
      121 aatgaaggcg aaacctgatc aagcaccaga ttcacaaggt ctcgccacca acctttgtca
      181 tcgtgcgggc gcggtccgtc gatggcggta cgccgtggca tctcgttcca agccttgtaa
      241 aacgcgcgat cgagctgtcg tgcatcgagc gtcaaaccta cttcgctgcc tacgagggcg
      301 taatcgtcgc cgaccgtcct ggttagatgc atgagagtgc caaccgcatc gaaaaagatc
      361 gccttgatca tcaaaagcaa cgttcaacgc ccaacgctca gcgttcaatg cccaagtaat
      421 ggtcgggcga gacttctgtc gagccgacga cgatctcggg aaaatcgatg gctctgtgat
      481 cgtcgcccta ccgttggaca acttcgtccc tccgttggat taatttgcat gctgccgact
      541 gagcataacg atccgattaa cgagaaaatc ctcgcgattt cggaggacaa gatcgacgga
      601 ttcgtgcgcg agccgttcga ggaaattgca cgacgctcag gtgtgagcgt cgatgttgtg
      661 atggcgcgca tcgccgccat gctgcgcgcg ggaacgatcc gccgtgtccg gcagactctg
      721 ctcgcaacta atctcgcgga tggcgccctg gtggcgtgga aagttcccga ggataagatc
      781 gacaacgcgt tcgactggat gtttcagcgc gatccctttt ccgggcatgt ggtcctgcgc
      841 tcgaccgata cggtaactgc aggatcggat tacaagttat ggacaaccgt gaaagtgcca
      901 cacggatttt ccttgcaaca acattgcgaa ttgctcgcga aaaaagtcgg cgcggagcgt
      961 ttccgagtga tgccggcgaa aggaattttc actctcggcg ttggtcatgt tcggcgaaaa
     1021 acgattgaac cggggagcag atcagaagag ccggcgaaga tgatccccgt gcaagttgtc
     1081 aatcttagcg agcaagaatg gaacatcctg ctcgcgttga agcgcgaatt tgttccggaa
     1141 gaaattcgac caacgccgtg ggcagctcgt gcagaggaag ccggcgtctc gcttgatgaa
     1201 ttttatcgag tggccgggca attaaacgcg cgcaagatca tcggccgctt ttcaactttc
     1261 ctggaacacg ttaaaccgtc tgctagtgga gtgcgggtca cgcgtttcaa cgcgcttttt
     1321 cactgggcgg tgccgccggg gcgcgaaatc gaggcgggcg gcgaagtcgg gcgacatcat
     1381 attctgacgc actgctattg gcgcgaagcc ggaccagaat tcaagaatgt aaacatcatg
     1441 gccgttgcgc acggaacgga taaacaactg ctgctcgagc acaaaggggc catcgaccgg
     1501 catcttcagg cgagcggcat cccggtctcc tatacaaacg ttttctgggg cgggcgcagc
     1561 gagatcaagc cgtcggaaat ttcaccgcat gtgtatcgcg agtggttagc agagcaggta
     1621 aaagagttga agcgttaagc caaagatgga tcaaccgctc cgcgggcgat gctatttaat
     1681 ttgcggcttg gccgcctgat ctaacatcga gcaagggact gctcgaacca ccaaagttct
     1741 ctttatagct gcgatccaga ttacgcagaa gagcaatggc aaacttctga cgatcaactt
     1801 gtcgacccaa ggccatcgcc agcgaaattg ccgggcctcg cagttctctg ggaaaatctt
     1861 ccaacgcctg atttacattg attccaattc ccacgatggc gagatgtcgc gctttttcct
     1921 gcgcgcgcat ctcgacgaga acgccgccaa ccttccggcc gtcaatctga acgtcattgg
     1981 gcaatttgat ggttgttttg agagaaaatt cgtcttcgat tgctcctgaa atagttttgg
     2041 ccgcccaggc ggtgagtagc ggggaacggt ttatctcgat cccgggtcgc aacaggattg
     2101 agaaccaaag cccttttgcc gccgccgatt cccaacgatt acctcgttgt ccgcgaccgg
     2161 cggtttgatg ttcggcgaag agaaccaggc cctcgtctga atttggtgtg gtgatttgca
     2221 agatcgcatc gttggtcgag ccggtttgat cgaggacgat aatttcgcgg ccaatgaccg
     2281 cgttgccgag agcggcttgc atctcatcag cggtcaactg ctctaatatt tcagccgggg
     2341 acatgtgtca tttccaagcc aatgtcgatt gcgcgcgcgg cgtgcgtgag gctacccacg
     2401 gaaatataat ccacgccggt ggccgcgatg cggttgacgt ttttgagagt gatgccgccg
     2461 cttgcttcga atttgatgtt gtctgttttg agcgcgacag cttcccgaat ttgagcgggt
     2521 gccatgttgt cgagcaggat tacgtctatt ccctgaattt cgacgaaggc acgaacctgg
     2581 tccagatcat cggcttccac ttcgatacgg atattttctc gctcctcgcg aatctgctta
     2641 attcggtccg caaaaccgga aagtccgtca agaatcatca aatgattatc tttcacgagc
     2701 accatatcgt ataaaccaaa acggtgatta cctccgccac cggcaagcac ggcggctttt
     2761 tccaggctcc gcagaccggg agtggttttt cgtgtatcca aaattttggc cggatgattt
     2821 ttcacggcgt cgacgaattg gcgtgtcagc gttgcgatac cacaaaggcg ctgtagaaaa
     2881 ttcaatgcca cacgttccgc cttaagaatc gagcgcgcca ggccacggac ctcgataatc
     2941 acatccccgc ctacaacttc gtcgccgtcg cggcgaagaa tctgagtgtc gattgacgga
     3001 tcgacttgtc ggaaaacttc ccccgcggct ccggtgccgg cgacaatagc tttttcacgc
     3061 gcaataatgc gacccgtcgc gtgcagtgtt gccggcacaa aaaaatcagt cgtgatatcg
     3121 ccttggccga tgtcttcttt gagtgcggcg gcgattggat cgtaatggtc gggcttcatt
     3181 ggcatcggcg cgtgctctag gctgataacg catcgcccac gcacgcaagt tttcaaatcg
     3241 gtgttaaccc ggcaccaaaa atgaaggtgc gagcaggtct tccctagttc gccaaccgga
     3301 ttgccgagat ttcaaacgcc ccgcgcgtcg agcaaggcta ccgtcgtttc ccaaatcacc
     3361 cggtacgaag cgtgacacgc agaaaaatgg cgaccttttg aaatcgtgtg gctatgtggc
     3421 gagatcgatt tgagacaaaa aaagattcgg gcagcacgcg cggtaaggag aaacaacgaa
     3481 tctagaaaca cgcgctacca cccgaatcgc cttaacctgt cgaattagac agcaacacgc
     3541 actgcccgtt cgaagttttt tacgaaaact tcgtgtctga ggctaaattc cgggcattta
     3601 ggcggttatg ccaaatcctt gcggcggcgg gtctctggcc gccgcccttg gcttggaagg
     3661 ccttcgaagg gaatttttgc gg
//
LOCUS       DASQUQ010000244         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1345087, whole genome shotgun sequence.
ACCESSION   DASQUQ010000244 DASQUQ010000000
VERSION     DASQUQ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345087"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VE486_06885"
     CDS             <1..420
                     /locus_tag="VE486_06885"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HZA38851.1"
                     /translation="WGSFDLAVTRFSGRAAPPHPGGVPHLPDEITREAYSQEVSSLGF
                     WPGNADAPTPIFYSYAYPEPAGFAEAKIQPPAASYQPKLREFILPYDAVRSAESPDNV
                     LLDFAQSTYDTASTLAGWDRTALDEVKPSLHSAQQHS"
     gene            430..1368
                     /locus_tag="VE486_06890"
     CDS             430..1368
                     /locus_tag="VE486_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZA38852.1"
                     /translation="MNPGCAAEQHAWREIKDGWRPLYGDVDKIGVAIEWHDFRTERSF
                     NWGRTFHRHSLEFCLNLNGHGAVGGEGQAQSDYLPGNSGYYAIADQPLPASRRAHDHH
                     QFVTLEFSREHLQKQFVQNEADLEPQIRRVVFDGREESVIAPVRPMTIEQRGVVASLA
                     EPPVAKAAQIIWYQSKALELMAHFLFAPKDPEFFCMRQKRVARERVERTKELLARDLA
                     NPPTLEMLGQEVGCSPFYLSRIFSREVGLTIPQYLRNVRMERAAELLRTGRYNVTEAA
                     TEVGYSSLSHFSKAFCETIGCCPVLYPAAKNVILDR"
     gene            1454..>2434
                     /gene="purH"
                     /locus_tag="VE486_06895"
     CDS             1454..>2434
                     /gene="purH"
                     /locus_tag="VE486_06895"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZA38853.1"
                     /translation="MRIRRALISVSDKTGVASFARALESQGVDIISTGGTADLLRKEK
                     IPVRDISSFTAFPEVLEGRVKTLHPRVHGGLLYKRGDAKHEAEARECGFEPIDLVAVN
                     LYPFESTVARPDVTLAEAIENIDIGGPSMIRSAAKNYEAVTVVVDPADYDAVLENMRD
                     NKGETTLKLRERLAIKAFIKTSEYDRAIGNFLNKEQATDSSFSLSLPLATRLRYGENP
                     HQSAALYGDFDKHFEKLHGKELSFNNILDISAATNLIGEFEAPTVAILKHTNPCGVGS
                     DANLRKAWDKAFATDKQAPFGGVIICNRPLDEPLAKVISEIFSEVIIAPDF"
BASE COUNT          545 a          650 c          702 g          537 t
ORIGIN      
        1 tggggcagtt ttgatctcgc agtaacccgt ttctcgggcc gagctgcccc gccgcatcct
       61 ggcggggtgc cacatctacc ggacgagatt acacgcgagg cttactcgca ggaagtgagt
      121 agccttggtt tctggccggg caacgctgac gctccgacgc cgatctttta ttcctatgca
      181 tatccggaac cggcgggatt tgcggaggcg aaaattcagc cgccggccgc ctcataccag
      241 ccaaaactgc gtgagttcat ccttccttat gacgcggtgc gcagtgccga atcacccgat
      301 aacgtcttgc tcgattttgc gcagagcacc tatgataccg cttcaacgct ggccggatgg
      361 gaccgcaccg cgctggacga agtcaaacct tccttgcatt cggcgcagca acattcgtaa
      421 ttaggcgaca tgaatcccgg ttgcgcggcc gagcaacacg cctggcgtga aatcaaagat
      481 ggatggcgtc cgttgtacgg cgatgtggat aaaattggcg tcgccatcga gtggcacgac
      541 tttcgcaccg agcgttcctt taactggggg cgcactttcc atcggcacag cctcgaattt
      601 tgtttgaacc tgaacgggca cggggcggtc gggggagagg gacaggcaca gagcgattat
      661 cttcccggca attccggtta ctacgccatt gccgatcagc cgctgccggc gtcgcggcga
      721 gcgcatgatc atcatcagtt cgtaacgctc gaattttcgc gtgagcattt gcagaagcaa
      781 tttgtgcaga acgaagcgga cctggagccg caaattcgcc gggttgtgtt tgatggacgt
      841 gaagagagcg tgatcgcgcc ggtgcgtccg atgacgatcg aacaacgtgg cgtagtggcg
      901 agtctggccg aaccacccgt ggcgaaagca gcccagatca tttggtatca aagcaaggcg
      961 ctcgaactca tggcgcattt tcttttcgca cccaaggatc cggaattttt ctgcatgcgg
     1021 caaaaacgcg tcgcgcgaga acgggtggag cggaccaaag aattgctggc acgcgatctg
     1081 gcaaatcctc ctacgctgga aatgctgggg caggaagtgg gttgcagtcc gttttatctc
     1141 agccgaattt tttcgcgcga ggtcggtctt actattccgc agtatctgcg caatgtccgg
     1201 atggaacgtg cggcggagct gttgcgcacg ggccgctaca atgtcaccga ggcagcgacg
     1261 gaagtgggat actcgagcct cagtcatttc agcaaagcgt tttgcgaaac gatcgggtgt
     1321 tgcccggtgc tttatccggc agccaaaaat gtcattcttg atcgctgacg cgatcgagaa
     1381 ctggagtgat ggagtggtgg agtaatgttt aaactgtttt aagcgttcat cactccaata
     1441 ctccattact ccaatgcgaa tccgacgtgc tcttatctcc gtttcggaca aaaccggcgt
     1501 cgcctcgttc gcgcgggccc ttgagagtca gggcgtagac atcatttcca ccggcggaac
     1561 agcggacctg ctgaggaagg aaaaaattcc tgtgcgcgac atttccagtt tcaccgcatt
     1621 tccagaagtg ctcgagggcc gggttaaaac tctgcatccg cgcgtgcatg gcggtcttct
     1681 ctacaagcgc ggtgacgcga aacatgaggc ggaggcgcgc gaatgcgggt ttgagccgat
     1741 cgatctggtg gcggtcaatc tttatccgtt cgaatcgact gttgccaggc cggacgtgac
     1801 cctcgcagaa gcgattgaga atatcgacat cgggggaccg tcgatgattc gtagcgcggc
     1861 aaaaaattat gaggccgtga cggtggtggt tgatccggcc gattacgatg ccgtgcttga
     1921 aaacatgcgc gataataaag gtgaaacgac gttgaaattg cgcgagcgcc tggccatcaa
     1981 agcgttcatt aaaacttctg aatacgatcg ggcgatcggc aatttcctga ataaagagca
     2041 ggcaaccgac tcgtcgtttt cactttcgct tcccctggcg acgcggttgc gttacgggga
     2101 aaatccgcat caaagtgccg cgctctacgg cgatttcgat aaacatttcg aaaaacttca
     2161 cggcaaggaa ctttccttta acaacattct cgacatcagc gcggccacga acttgatcgg
     2221 cgaattcgaa gccccgactg tcgcgattct taaacatact aacccgtgcg gcgtcggttc
     2281 agatgcgaat ctgcgcaagg catgggacaa agcgttcgcg acggacaagc aagcgccctt
     2341 cggcggggtc atcatttgca accggccgct tgatgagcca ctggccaaag tgatcagtga
     2401 aatttttagc gaagtaatca tcgcccccga tttc
//
LOCUS       DASQUQ010000245         5351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_534481, whole genome shotgun sequence.
ACCESSION   DASQUQ010000245 DASQUQ010000000
VERSION     DASQUQ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534481"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /gene="hutU"
                     /locus_tag="VE486_06900"
     CDS             <1..1230
                     /gene="hutU"
                     /locus_tag="VE486_06900"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZA38854.1"
                     /translation="TFAAAGRKHFGGSLEGKFVLTGGLGGMGGAQPLAATMNGAVFLG
                     VEVDPARIEKRLKSGYCDKIAHSLDEALRMIDVARKDRRSMSVGLVGNCADVLPEIVK
                     RGIIPDVLTDQTSAHDALNGYVPHGMSLEDALMLRRKSPEEYIERAMHSMAVHVEAML
                     ALQKKGAVTFDYGNNIRAQAKKVGVENAFDIPGFVPEYIRPLFCEGRGPFRWVALSGD
                     PEDIARTDQLALELFPENKTLARWMRLAKERIQFQGLPARICWLGYGERAEFGMAMNE
                     LLKSGEIKAPIVIGRDHLDSGSVASPNRETEGMIDGSDAIADWPLLNALINTAAGASW
                     VSIHNGGGVGIGYSQHAGMVVVADGSENSKHRLERVLTSDPGLGILRHADAGYSRAIE
                     FAAERKMDIPMQPRARD"
     gene            1294..1515
                     /locus_tag="VE486_06905"
     CDS             1294..1515
                     /locus_tag="VE486_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38855.1"
                     /translation="MATITEVEKLALDLPQNQRAVLAAHLLGSLPPVLHDEDEGIAEA
                     LRRDAELDANPSSGILLKELDGQIERRKS"
     gene            1515..1808
                     /locus_tag="VE486_06910"
     CDS             1515..1808
                     /locus_tag="VE486_06910"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZA38856.1"
                     /translation="MRLVLHPKVYSDIDRIMAYYEEVATPELANEFYAELRRFMREAV
                     VRPESFAFCERDIRRVNLYRFPYHFLFRIAGDAVRILVVRHHSRHPSLGTGRR"
     gene            1805..3493
                     /gene="ftcD"
                     /locus_tag="VE486_06915"
     CDS             1805..3493
                     /gene="ftcD"
                     /locus_tag="VE486_06915"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HZA38857.1"
                     /translation="MKKLIECVPNFSEGHDPDIIRQITDAIESVEGVSLLDVDPGAST
                     NRTVVTFVGSPDAAVEAAFRGIKKAAELIGMRKHKGAHPRMGATDVCPFIPVSNVTWE
                     EAIACANGLGKRVGDELNIPVYLYEKAAKDKARSNLSVIRAGEYEGFFEKIKQPEWKP
                     DFGPSVFNEKSGATVIGVRDFLVAYNVNLNTKSVRRANSVAFDVREQGRTKTEDGTPY
                     GKPVLDSSGEPVRVPGMLKHVKAIGWFVKEYGIAQVSMNLTNIEETPLHAAFDACCEA
                     AANRGLRVTGSEIVGMVPKKCLVEAGRYFLRKQKWSEGASEEELIDLAIRSMGLGELK
                     PFDPEEKVIEFKIESSAAQKSLLKMDLRQFCNETLSDSPAPGGGSVAALMGALGASLG
                     GMVANLSAGKRGWDDKLSYFSDWAVKAQQLKDELLSLVDEDTAAFNKVMDAFALPKDS
                     ADEKKARSAAIEQATKHAAEVPLKVMEAAFKSYELLSEMAEKGNPASISDVGVGILAV
                     RACIGGAALNVRINLGQLKDEKFRSALLKKVQELSADSDARFNKINQIVESKLG"
     gene            3587..5098
                     /locus_tag="VE486_06920"
     CDS             3587..5098
                     /locus_tag="VE486_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38858.1"
                     /translation="MRWFANKISFPSAYAVLMLVIVLGAALTWMLPAGRYDLLRYDQA
                     HRSFVAERATGEETLPSEQATLDRLGIQIRIENFEKGNIRKPISIPGTYHAVTANRQG
                     IIAILKAPVLGTYEAIDVILFVLVIGGFIGVFQKTGAFDAGLNALVSRLRGREAWLIV
                     IVTTLISLGGTTFGMAEETLAFYPLLVPVFLAAGYDAMVSVAVIFIGTCVGTMASTVN
                     PFAIIIASNAAGISWTSGLPGRVIMWTLATGVSVAYILRYAKRVQLDPTRSLTTGHSH
                     LPAAEPDTIKSQRKLTPKLRLLLAVFALTFAVMIFGVSRLDWWFPEMTTLFFAAALAI
                     GIIDRPGEKVFTETFIAGAQNLLGVGLIIGIARGATLILDKGEISGTILHYLSGRVEH
                     VPGVAFIVGLFFVYTVLSIFIQSSSGMAVLTMPIMSALAGVAGVSREEIVNAYCYGTG
                     VMSFIAPTGLVLPSLAMVNVRFDKWVRFTWPLMALLAALSVLVLALGVLLQRG"
     gene            complement(5141..>5351)
                     /locus_tag="VE486_06925"
     CDS             complement(5141..>5351)
                     /locus_tag="VE486_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38859.1"
                     /translation="AMGYTSIPSQANFIMFDCKRPVVPLIQALKQRNVQVGRLFPALP
                     NYMRLTIGKKSEMQSFLSAFREVAA"
BASE COUNT         1175 a         1375 c         1604 g         1197 t
ORIGIN      
        1 acgtttgccg cagcgggccg taaacatttt ggcggatcgc tcgaaggaaa atttgtgctt
       61 accggtgggc tcggcggaat gggcggcgca caaccgctcg ctgcgactat gaacggagcg
      121 gtttttctcg gcgtcgaagt cgatcctgcg cggatcgaaa agagattgaa gagcggttat
      181 tgcgataaaa tcgcgcactc gctggatgaa gcgctgcgga tgatcgatgt cgcgcgcaaa
      241 gatcgacgct cgatgtcggt cggtctcgtc ggcaattgcg ccgatgtgct gccggagatc
      301 gtcaagcgcg ggatcattcc ggatgttctt accgatcaga ccagcgcgca tgacgcattg
      361 aacggctatg tgccgcacgg gatgtcgctc gaagatgcgc tcatgttgcg gcgaaaaagt
      421 ccggaagaat atatcgagcg tgcgatgcac tcgatggctg ttcacgtcga ggcgatgctg
      481 gcgttgcaaa aaaaaggcgc cgtcactttc gattacggca acaacattcg cgcccaggcg
      541 aaaaaggtcg gcgtcgagaa cgcgttcgat attccagggt ttgttcccga atacattcgt
      601 ccattgtttt gcgaaggccg tggtcccttc cggtgggtcg cgttgagcgg tgacccagaa
      661 gatatcgcgc gcaccgatca gctcgcgctc gaattatttc ccgaaaacaa aactctcgcc
      721 cgctggatga ggctcgcgaa agaacggatt caatttcaag gattgccggc gcggatttgc
      781 tggctcggct atggcgagcg cgccgaattt ggtatggcga tgaacgagct gctaaaaagc
      841 ggggaaatca aagcgccgat tgtgatcggc cgcgatcatc tcgattccgg ctcggtcgca
      901 tcgcccaacc gcgaaaccga agggatgatc gatggcagcg atgcgatcgc cgattggccg
      961 ttgctgaacg cgcttatcaa tactgccgcc ggcgcctcat gggtgtcgat ccacaatggc
     1021 ggcggggtcg gcatcggtta ttcgcagcac gccggcatgg tcgtggtcgc cgatgggagt
     1081 gaaaattcca agcatcgtct ggagcgcgtc ctgacaagcg acccaggatt gggcatcctt
     1141 cgccatgccg atgccggcta ctcccgcgcc atcgaattcg cagccgagcg caagatggat
     1201 attccgatgc agccgcgcgc gagagattga tccaaaccag ctgcgaagtt gccatgcttg
     1261 ctcggcaaag cgccgccgat tatactcggg cgcatggcca caattaccga agtcgagaag
     1321 cttgcgctcg atttgcctca gaatcaacga gccgttcttg cagcgcatct gcttggatcg
     1381 cttccgccag ttcttcacga cgaagacgag ggaatcgctg aagcgctccg gcgggatgcg
     1441 gagcttgatg cgaatccttc gtccggcatc ttactaaagg agctcgacgg acagatcgaa
     1501 cgccgcaaaa gctgatgcgg ctcgtccttc atccgaaggt ttactcggat atcgacagaa
     1561 tcatggccta ttacgaggaa gtagccacac ctgaactcgc aaatgaattc tatgcagagt
     1621 tgcggcgttt catgagagag gcggtcgtgc ggccggaatc tttcgcgttt tgcgaacgcg
     1681 atattcggcg agtgaacctt tatcgctttc cttatcattt cctttttcga atcgccggcg
     1741 acgcggttcg aattctggtt gtccgtcatc acagcagaca tccatctctg ggcaccggac
     1801 gccgatgaaa aaactgatcg aatgcgtgcc gaatttctct gaagggcatg atccggatat
     1861 tattcggcag attacggacg cgattgaatc ggtggagggc gtttcgttgc tcgatgtcga
     1921 tccaggcgcg agcacgaatc gcacggtcgt gacgttcgtc ggcagtcctg atgcggcggt
     1981 cgaagcggca tttcgcggca tcaaaaaggc cgcggagctc atcggcatgc gcaaacacaa
     2041 gggcgcgcat cctcgcatgg gcgcgaccga tgtttgtccg tttattccag tgagcaacgt
     2101 cacttgggaa gaagcgatcg cttgcgcaaa cggtctcgga aaacgcgtcg gcgacgaatt
     2161 aaacatccct gtctatctct atgaaaaggc ggcgaaagat aaggcgcgct cgaatctttc
     2221 ggtgattcgc gcgggtgaat acgaaggatt cttcgagaaa ataaaacagc cggagtggaa
     2281 gccggatttc ggcccgtccg ttttcaacga aaaatcgggc gcgacggtga ttggcgtccg
     2341 tgattttctc gtcgcttata atgtgaacct gaacacgaaa tcggtgcgac gcgcgaattc
     2401 ggtcgcgttc gatgtgagag agcaggggag gaccaagacc gaagatggca cgccctacgg
     2461 aaagccggtg ctggattcga gcggcgagcc tgtccgcgtt cctggcatgt taaaacacgt
     2521 caaggcgatc ggctggtttg tgaaggaata tggaattgcc caggtctcga tgaacctgac
     2581 gaatattgag gaaacgccgc tgcacgctgc attcgacgca tgctgtgaag cggcggccaa
     2641 tcgcggtttg cgcgtaacag gttcggaaat cgtcggaatg gtcccaaaga aatgtctggt
     2701 ggaggccgga agatattttc ttcggaaaca gaaatggtcg gaaggcgcgt ccgaggaaga
     2761 gttgatcgat cttgcgatcc gctcgatggg cctgggtgaa ttgaaacctt tcgatccgga
     2821 agaaaaggtg attgaattca agatcgaatc ctccgccgcc caaaaatcac tcctcaaaat
     2881 ggatctgcgt cagttctgca acgaaacctt gagcgattct ccggcgcccg gcggagggtc
     2941 agtggcagca ttgatgggag cattgggtgc ttcgcttggt ggaatggttg ccaatctctc
     3001 cgctggcaaa cgcggctggg acgacaagct aagctacttc agcgattggg cggtgaaggc
     3061 gcaacaattg aaggatgaac tgctctcgct ggtcgatgaa gacacggccg cgttcaacaa
     3121 ggtgatggat gcattcgcgt tgccaaagga ttcggccgac gaaaaaaaag cgcgttccgc
     3181 ggcaatcgag caggcaacca aacatgcggc cgaggttcca ttgaaagtca tggaggctgc
     3241 attcaaatct tacgaacttc tttccgagat ggctgagaaa ggaaatcccg cttcgatttc
     3301 agatgtgggc gttggaatcc tggctgtgcg tgcctgcatt ggcggcgcag cactgaatgt
     3361 ccggataaac ctgggccagc tcaaagatga gaaatttaga tcggcgctcc tgaaaaaagt
     3421 tcaagagctt agcgccgatt ccgacgcgcg gttcaataaa atcaatcaaa ttgtggaaag
     3481 caaactaggc taggaacaaa tctccgaaat agtgtgggcg gcttttatga aaaatcgagc
     3541 tgctgccaag ttgtccatct ccagctggag ataacgcgct gttcgtatga gatggttcgc
     3601 aaacaagatc agtttccctt cggcctacgc ggtcctgatg ctggtgatcg tgctcggagc
     3661 ggctctgacc tggatgctcc cggccggacg ctacgatctg ctccgttatg atcaggcgca
     3721 tcgcagtttc gtggcggaac gcgcgaccgg cgaagaaact ttgcccagtg agcaagcaac
     3781 gctcgatcga ctcgggatcc aaattcggat cgagaatttt gaaaaaggga acatccgaaa
     3841 gccgatctca attcccggca cgtatcacgc ggttaccgca aatcggcagg gcataattgc
     3901 aattttgaag gcgcctgtgc ttgggactta tgaggcgatc gacgtaatcc tgttcgttct
     3961 ggttatcggc ggcttcatcg gggtcttcca gaaaacaggc gcgttcgatg ccgggttgaa
     4021 cgcccttgtg agtcgcctcc gtggacgcga ggcctggttg atcgtcattg tcaccaccct
     4081 gatctcgctc ggcggaacga ctttcggaat ggcggaggaa acactggcgt tttatccatt
     4141 gctcgtcccg gtttttctcg ctgccggata cgacgcaatg gtgtcggtcg cggtcatttt
     4201 catcgggacc tgcgtgggaa caatggcctc caccgtaaat ccgttcgcga tcatcatcgc
     4261 atcaaacgcg gccggtattt cctggacgag cggactgcct ggtcgcgtga tcatgtggac
     4321 gctggcgacc ggggtcagcg ttgcatacat tttgcgttac gcgaaaaggg tgcagctcga
     4381 cccgacgcga tcgcttacga cggggcacag ccatctgccc gcggcagaac cggacacgat
     4441 caaatcacag cgcaagttga ccccaaagct gcggctcctg ctcgcggtgt ttgcgttgac
     4501 attcgccgtg atgatctttg gcgtttcacg tctcgactgg tggtttccgg aaatgaccac
     4561 cctgtttttt gcggcggcac tggcgatcgg aatcatcgat cggccaggcg aaaaggtttt
     4621 cactgagacg ttcattgccg gtgcacaaaa tcttctcggc gttggattga ttatcggcat
     4681 cgcgcgcggc gcgacgctca ttttggataa gggagaaatc agcggcacaa ttctgcatta
     4741 cctgtccggc cgcgtggagc acgtgcccgg tgtggcgttc atcgtggggc ttttcttcgt
     4801 ttacactgtg ctctcgatct ttatccaatc gtcgagcggc atggccgtgc tgacgatgcc
     4861 gattatgagc gcactggcag gggtggccgg tgtgtcccgc gaggaaatcg tgaacgcata
     4921 ctgctacgga actggcgtaa tgagctttat cgcgcccact ggtttagtcc tgccctcgct
     4981 cgcaatggta aatgtacgat tcgataaatg ggtgcgtttc acctggccgc taatggcgct
     5041 gctggcagcg ctctcggtcc ttgttctcgc gcttggggtt ttgttgcagc gtggctagtt
     5101 ggcgtatgtc cccgacgaag atgttgacgg tggcgtgttg ttaggctgcg acttcccgaa
     5161 acgctgagag aaaactttgc atctcggatt tcttgccaat ggtcaggcgc atgtaattcg
     5221 ggagtgccgg aaataagcgg ccgacctgca cattgcgctg ttttaacgcc tggataagcg
     5281 gcacgacagg acgtttgcag tcgaacatga tgaagttggc ttgtgacgga attgatgtgt
     5341 agcccatcgc g
//
LOCUS       DASQUQ010000246         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1149619, whole genome shotgun sequence.
ACCESSION   DASQUQ010000246 DASQUQ010000000
VERSION     DASQUQ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149619"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VE486_06930"
     CDS             complement(<1..369)
                     /locus_tag="VE486_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38860.1"
                     /translation="MMDYGVSPYDDPYARPQRGTNYFAWTVAILLLTGFALAAWLGSF
                     YIFNQPERPDSYRILQRLHKIDPPKRFQLTAAPAGEFLSAKQLYDRYIGMGSAELARA
                     NAELARNYIRNYQLVRGLVPY"
     gene            complement(395..730)
                     /locus_tag="VE486_06935"
     CDS             complement(395..730)
                     /locus_tag="VE486_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZA38861.1"
                     /translation="MINVTDNAVRQLRILLAAQGENSRKGLRVQIAKGGCSGLQYEMA
                     LDEKKEGDAVVERDGTRFLIDSESIPYLRDATLDFHDGLTGAGFHIVNPNASRTCGCG
                     SSFETARPA"
     gene            957..>2000
                     /gene="mutL"
                     /locus_tag="VE486_06940"
     CDS             957..>2000
                     /gene="mutL"
                     /locus_tag="VE486_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZA38862.1"
                     /translation="MSRIRLLPDTVASQVAAGEVVERPASVVKELVENSIDAGARKIE
                     IMIRRGGISLVRVIDDGCGMDRDDALLSLERHATSKIRSAADLQAVATLGFRGEALPS
                     IASVSRFRLTTREPNAIAGTEIIVNGGKIDIVRESGEAPGTQTEVRSLFYNLPARRKF
                     LRSENTESRNIEHQIHLQAIGHPQIAFTLLRDDRLVFQLPGTATLRHRIRDLYGVALL
                     ERLIEVKGNGPRKIQISGYVGQAGLSRQTRAQQLIFVNGRAIESGLITGAIREGYHTA
                     LMKGQYPVTFLFLDLDPAAVDVNVHPAKREVRFRDPTGVREAIVGYVQQTLERARIQW
                     QEKFRAPAMPPSAI"
BASE COUNT          444 a          556 c          566 g          434 t
ORIGIN      
        1 gtaaggcacc aggccgcgca ccagctgata atttcgaata taattgcggg ccagttcggc
       61 attggctctg gccagttcgg cgctgcccat cccgatgtag cgatcatata attgtttcgc
      121 gctcaggaat tcgcccgccg gtgcggccgt aagctgaaaa cgtttcggtg gatcaatctt
      181 gtgcagcctt tgtaaaatcc ggtagctatc gggacgctcc ggttgattga atatgtaaaa
      241 acttcccagc catgctgcca gagcgaagcc agtcaggagc agaatggcga ctgtccacgc
      301 aaaataatta gttcctcgct gtggacgagc gtagggatcg tcgtatgggg aaacaccata
      361 gtccatcacg ttaacgcgag aagatttctc cgtttcaagc tggtcgcgca gtttcgaacg
      421 aggagccgca cccgcaggtc cgcgacgcgt ttggattgac gatgtgaaaa ccggcgccgg
      481 ttaaaccatc gtgaaaatcc agggtagcat cacgcaagta tgggatgctc tcgctgtcga
      541 taagaaatcg ggtcccatcc cgttccacca cggcgtcgcc ctcctttttc tcatcgagcg
      601 ccatctcgta ttgcagaccc gaacagccgc cttttgcaat ctgcacgcgc agacccttcc
      661 gggaattttc tccctgggct gcaagcaaaa ttcggagctg tcgaacagcg ttgtccgtga
      721 cgttaatcat ctggacagaa aatacggtac cggcgtatcc aagtcaaaac tggcgcgatc
      781 actcccgctg ggcggaagct gtagccacgg cgctgtgccg ccgtgcgggt ggcatgcagg
      841 ctgagcgcct cgacacagcg aggcggctac aacaacgctg gaccgatatt accgcagact
      901 gttttggtct ttttcttcgc aatcggcgat ctggaaccta gaatctggag tacaaaatga
      961 gccggatccg tttgctgcca gatactgtcg ccagccaggt cgccgcgggc gaagtggtgg
     1021 agcggcccgc ctccgtggtg aaagaactgg tagaaaacag catcgatgcc ggtgctcgca
     1081 aaattgagat catgattcgc cggggaggaa tttcgcttgt tcgcgtgatt gacgacggat
     1141 gcggcatgga tcgtgatgac gcgctgcttt cgcttgagcg gcatgctacg agcaagattc
     1201 ggtcggccgc tgatcttcaa gccgttgcca cgctgggttt ccgaggcgaa gcattaccga
     1261 gcatcgccag tgtttcgcga tttcgattaa ccacgcgcga gcctaatgcc attgccggaa
     1321 cggagatcat tgtcaatggc ggcaagatcg acatcgttcg cgagagcggt gaagcgccgg
     1381 gaacgcagac cgaagtgcgc tctctttttt ataacctgcc tgcgcgccga aaatttcttc
     1441 ggtccgaaaa cacggaaagc cgcaatatcg agcatcagat ccatttgcag gcgattggcc
     1501 atccgcagat cgcgttcacg ttgctacgag acgaccgtct cgtttttcaa ttgccgggga
     1561 ccgcaacgct tcgccatcga atccgggatt tatatggggt cgcactgttg gaacgtctga
     1621 ttgaggtaaa gggcaacggt ccacggaaga ttcaaatcag cgggtacgta ggccaggctg
     1681 gtctcagccg gcagacacgc gcgcaacagc tcatttttgt caacggccgc gcgatcgaaa
     1741 gcggcctcat caccggcgct attcgtgagg gctatcacac tgcgctcatg aaagggcaat
     1801 acccggtcac tttccttttt ctcgatctcg atccagccgc agtggatgta aacgttcatc
     1861 cggccaaacg cgaagtgcgc tttcgggatc cgaccggagt tcgcgaagca atcgtcggtt
     1921 acgttcagca aaccctggag cgcgctcgga tccagtggca ggaaaaattt cgcgcaccgg
     1981 ccatgccacc gtccgccatt
//
LOCUS       DASQUQ010000247         4082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_431134, whole genome shotgun sequence.
ACCESSION   DASQUQ010000247 DASQUQ010000000
VERSION     DASQUQ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431134"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VE486_06945"
     CDS             complement(<1..453)
                     /locus_tag="VE486_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZA38863.1"
                     /translation="MRRSLRFFQAVHFRAALYIRSPKMDEENELIALRRKKLDALRAK
                     GIEPFGSAFESSGAIAEVREKFKEGETLFAAGRITGHRDMGKSHFLDLRDATGRIQIY
                     VQVKEVGPELIDLFRLLDMGDLIGVEGTCFLTKSGEPTLKVRAFQLLAK"
     gene            624..1094
                     /locus_tag="VE486_06950"
     CDS             624..1094
                     /locus_tag="VE486_06950"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38864.1"
                     /translation="MTVDLPETTSKMEGPLVKQFSVFLPNKVGAMLDLVKLLNTQNTS
                     VLALSVSESTDSAIARIIVSDPERVEELFQEKNIAFGVCEVVIVEMREVATQLAKLLA
                     ALVMAEVNVHFAYPLLTRPRGFAAIALHVDDTDCASSVLMGEGFKILSQSDISR"
     gene            1289..2386
                     /locus_tag="VE486_06955"
     CDS             1289..2386
                     /locus_tag="VE486_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38865.1"
                     /translation="MRSLLSRATQADPSFSIGLGLIVMLAIAEIFAATFYYVGRARAA
                     RASAQAIAASVLSPPAHSTTFTPAPAEPAVSPLVAAPTPPPSLVDQLLREGIELRERG
                     DTTTALARLQEASESDPTNVAVLEEIAKTYEAMQLFERSNEVWRKLQEMGPSAGAAYE
                     TADQRLKLGVPTPAPVGAGIASASLDGAAPNKDVGGGITEGSIFGITEVKTTETPDPD
                     AEANLTLQIGIKKQPDATIDDTKVKIQVFFYDMVDNRDIKLTDAEVNYEWLTPKHDWT
                     ETNPEILTVSYLRPKTRVVSSEAALSEAAASVKPGQKGRPARSSTGEAGQRKYLGYKI
                     LLYYDDKLQAVQAEPARLLQLFPPSEKISPP"
     gene            2621..3508
                     /locus_tag="VE486_06960"
     CDS             2621..3508
                     /locus_tag="VE486_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38866.1"
                     /translation="MNRCVLFMVCVFLFAAGAQGQIQVDLKFKRLQYIAYEPVMATLG
                     ITNLAGRDINLHDTDGQAWFGFEVTGSEGQPIAPTAADSTQAPLKIEAGQKVTQRIDL
                     AALYPVHDFGTYHVRAHIYFPDLGKFFYSPSKVFEVTDARPIWQQTVGIPDGVAAPGE
                     ARTYSLLTNRFPDHTSLYVRVQDRDKGVVYATYSLGRTIAFEQPQAEIDRANQLHVLH
                     CAAPRAWTYSRVGLNGELLGHSSFMETKTRPRLARSTSGEVAVRGGVIEAPAAQTKTP
                     RKTAPKLSDRPPGMPRENR"
     gene            3508..>4082
                     /locus_tag="VE486_06965"
     CDS             3508..>4082
                     /locus_tag="VE486_06965"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZA38867.1"
                     /translation="MPGNFSQLYLLGAVLFIADLTCATSASAASRRRDSQETGSSPTT
                     VVIDAGHGGHDRGGVPGQRIPEKEMTLEVAQRLKKMLAADGYRVVMTRENDVFVPLGT
                     RVAIANSYRNAILVCIHFNSAKRVGADGIETYFYGRDSLPLASAVHYFVAGGAPSMNR
                     GVRRRGYYVLRKARVPTVLVECGFLTNPTEAA"
BASE COUNT          907 a         1126 c         1174 g          875 t
ORIGIN      
        1 tttggccagg agctggaacg cgcgcacttt tagtgtcggt tctccgcttt tggtcaggaa
       61 acatgttccc tcgacgccaa tgaggtcgcc catatcgagc aggcgaaaga gatcgatcaa
      121 ctccggacca acttctttga cctgaacata aatttgaatg cgtccggtgg catcgcgcag
      181 atcgagaaag tgactcttgc ccatgtcgcg gtggccggtg atacggccgg ccgcgaaaag
      241 ggtttcgcct tctttgaatt tttcgcgcac ttccgcaatg gctccgctcg attcgaaagc
      301 ggagccgaac ggctcgatcc ctttcgcgcg aagcgcgtcg agttttttgc gccgcagcgc
      361 gattagctcg ttctcttcgt ccatcttcgg cgagcgaatg taaagcgccg cgcggaaatg
      421 aacagcctga aagaagcgca ggcttctacg cattgaagcg ttaaaaagtt acaatggccg
      481 ggtctcccgc tgtaacgatg taacctttta gcgaatcacg tgggtgcggc agcgccaaca
      541 cattgcgttt gccaacgaaa gatcgaccgt gagatagtta ccgatgctcg acgccgattc
      601 tttctcggcg cgctgcgacc cacatgaccg tcgatttacc ggaaacaact tcgaagatgg
      661 aaggaccgct ggtgaagcag ttttccgttt ttctgccgaa caaagttgga gcaatgctgg
      721 atcttgtaaa actgctcaac acccaaaaca cttctgtgct ggctttgagc gtctctgaat
      781 ccaccgattc agcgatcgcg cggattatcg tgagcgatcc cgagcgggtg gaagaactgt
      841 ttcaggaaaa aaacatagct ttcggcgttt gtgaggtggt gatcgtggaa atgcgcgaag
      901 tcgcgaccca actggcaaag ctccttgccg cactggtgat ggccgaggtc aacgtccatt
      961 tcgcttatcc tctgcttaca cggccgaggg gctttgccgc gatcgccctg cacgtggacg
     1021 atactgattg cgcttcctca gtgctcatgg gcgaaggatt caagattctc agtcagagcg
     1081 acatttcgcg atagagcgat tgattcgtcg ggtcccattc ggcaggcgca agctgagtct
     1141 cgcctcagta gcaaattggt agggcgtgac cgccgggcgc gcccccacga ttgaatcacg
     1201 agcaagatca cgatcacgag caggagggac ccctccgcga gttgacagat ttggggaagc
     1261 gcgtttacag agttgcgtcc atcgcgccat gcgaagtctt ctctcacgcg ctacacaggc
     1321 cgacccaagt tttagcattg ggctcggctt aattgtcatg ctagctatcg cggagatttt
     1381 cgcggcaacg ttctattatg ttggccgggc tcgcgccgcc cgggcatcgg cgcaggcaat
     1441 cgcggcaagc gtgctaagcc ctccggcaca ttctacgacg ttcacgccag ccccggccga
     1501 accagcggtt tcgccgttgg tggcggcccc gactcctccc ccgtccctgg ttgatcagct
     1561 gctgcgggaa ggcattgagt tgcgtgagcg gggagatacg acgactgcgc tcgcccgttt
     1621 gcaggaagca tcggaaagcg atccgacaaa cgtcgcggtg ctcgaagaaa tagccaagac
     1681 ctacgaagca atgcagctct tcgaacgctc gaatgaagtg tggcgcaaac tacaggaaat
     1741 gggtccgtca gccggcgcgg cgtatgaaac ggcagaccag cgtttgaagc tgggagtccc
     1801 gactcctgcg cccgtggggg ctggcatcgc aagcgcgtca ctcgatggag cagcccccaa
     1861 caaagacgtt ggtggtggaa tcacagaagg ttccatattt ggcatcactg aagtcaaaac
     1921 gaccgagacc cctgatccgg atgctgaggc gaacctgacg ttgcaaatcg gcataaaaaa
     1981 acagccggac gccacaatcg atgacacgaa ggtgaaaatt caggtgttct tttacgacat
     2041 ggtggataac agagatatta agctgaccga cgctgaagtg aattatgaat ggctaacgcc
     2101 gaagcacgat tggacggaga caaacccgga gatcctcacg gtgagttacc tgcgcccgaa
     2161 aacgagagtc gtttcgtccg aggcagcgct gtctgaggca gcggcgtcgg tcaagccagg
     2221 acagaagggt cgaccggcca ggagttccac tggagaagct ggtcaacgaa aatatctggg
     2281 ctacaagatt ttgctttatt acgatgataa attgcaagcc gttcaggcgg aaccggcacg
     2341 actgttgcag ctttttcctc cttcggagaa aatctcgccg ccgtgatgcc attgccgtcg
     2401 tcccgtcaaa taacgaagca cgaatgacga atgacgaatt aatgacgaag gtctggcctt
     2461 cgcttagcta cggcacggca ggcgaatgac caaaagctcg gctaaaatcg acatgctaat
     2521 tcgggcttcg tcattaggca ttccttcgtc attcgcctgc cgcgccgtag gtcgccgcgg
     2581 cgaccgaagg cgggtcattc ggatttcggt attaaggaat gtgaacagat gcgttctttt
     2641 catggtctgc gttttccttt ttgctgctgg agcgcaaggg cagattcagg tggacctgaa
     2701 attcaagcga ttgcagtaca ttgcctatga accggtgatg gcgacactgg ggatcaccaa
     2761 tctggctggg cgcgatatca atcttcacga cactgatggc caggcgtggt ttgggtttga
     2821 ggtaactgga agcgaaggtc agccgattgc gcccaccgcg gcagatagca cgcaggcgcc
     2881 cttgaaaatt gaagcggggc aaaaagtcac gcaacgaatc gatcttgccg cgctttatcc
     2941 ggtgcacgat ttcggcactt accacgttcg tgcccatatt tatttcccgg atctggggaa
     3001 gtttttttat tcgccatcga aggtatttga ggtaaccgat gcgcggccca tctggcaaca
     3061 aacagtggga atccccgatg gggttgccgc acccggagaa gcccgcacct actcgctgct
     3121 gaccaaccgg tttccagatc atacctccct ttacgtgcgc gtgcaggacc gggacaaggg
     3181 cgtcgtctat gcgacttatt cgttaggccg aacgattgcc ttcgagcaac cgcaggcgga
     3241 aatcgatcgc gcgaatcaat tgcacgtcct gcattgtgcg gctcctcgag cctggaccta
     3301 ttcccgagtc ggtctgaacg gcgaactgct tgggcactct tcttttatgg aaaccaaaac
     3361 gcgcccgcgc ctggcccgtt ccaccagcgg tgaagtggcg gtgcggggcg gcgtgatcga
     3421 ggcgcccgcc gcgcaaacaa aaacaccccg taagacggcg ccgaaactct cggaccgccc
     3481 gccgggaatg cccagggaga atcggtgatg ccaggaaact tttcccaact ctatctgctt
     3541 ggtgctgttt tattcattgc agatctgacg tgcgccactt ccgcgtcggc cgcctcccga
     3601 cgacgggaca gccaggaaac cggaagctcg cccaccactg tcgtaattga tgccggtcac
     3661 ggcggtcacg accgcggcgg tgttcccggg cagaggatcc cggaaaagga gatgacgctc
     3721 gaggtcgcgc aacgccttaa aaagatgctc gccgcggacg gctatcgcgt cgtcatgacc
     3781 cgcgagaacg atgtttttgt tcccttggga acacgggtgg caatcgccaa ctcgtatcgc
     3841 aacgctattt tagtgtgtat ccattttaat tcagcgaaac gcgtcggcgc cgatgggatt
     3901 gaaacatatt tttatggccg cgacagtttg ccgcttgcct ccgccgttca ttattttgtc
     3961 gcaggcgggg cgccgtcaat gaatcgcgga gttcggcgtc gcggttacta cgtcctgcgc
     4021 aaagcacggg tgccgacagt attggtggaa tgtggcttcc tcaccaaccc gaccgaagca
     4081 gc
//
LOCUS       DASQUQ010000248         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_880609, whole genome shotgun sequence.
ACCESSION   DASQUQ010000248 DASQUQ010000000
VERSION     DASQUQ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880609"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VE486_06970"
     CDS             complement(<1..559)
                     /locus_tag="VE486_06970"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZA38868.1"
                     /translation="MNISAVLLAGGQSRRMGRDKAIILFRGKPLWQIQLGLLRKLQPA
                     EIFVSARTDLDWRPPDATFVPDEPPSHGPLGGLTATLARIQSSHLLALAIDMPLMNEG
                     HLRFLCSQIQRGCGVLPMIGDLAEPLAAIYPAEACDTFSAALSGNDFSLQALTNQLVK
                     TGKLRAVRVPEEEQRCYWNFNEPADM"
     gene            complement(624..1151)
                     /locus_tag="VE486_06975"
     CDS             complement(624..1151)
                     /locus_tag="VE486_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38869.1"
                     /translation="MEICHLYISPGHNFVGHHGRKPGDYPAVEVPMIECVAGRGIRSD
                     RYFDFKDDYKGQITFFSLEVFDELCGALELHGCSPALVRRNVMTRGVDLNELIGRDFE
                     VQGVHFHGTQECSPCYWMNQAIAPGAEEFLKGRGGLRAKILCDGELRSTAANCHPERS
                     EGPHLRSLRYARDRA"
     gene            complement(1447..1695)
                     /locus_tag="VE486_06980"
     CDS             complement(1447..1695)
                     /locus_tag="VE486_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38870.1"
                     /translation="MSTVAEIEAAIKALPPEERERLAENLPSILPELRGDLVWQHIIG
                     DKRPRPALSALGDEVEAQFKANANRFPEIKESDFEQRS"
     gene            1765..2583
                     /gene="fdhD"
                     /locus_tag="VE486_06985"
     CDS             1765..2583
                     /gene="fdhD"
                     /locus_tag="VE486_06985"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZA38871.1"
                     /translation="MKENGINTQKIIRRKGDGELEYQPDDLTIEEPLEIRIGRKALAT
                     TMRTPGHDEELAAGFLVSEAIVRKPGEVAKISTNGDNIVTVALAAGVRPQLNFAERFG
                     TISSSCGLCGKNSIEAIRQNFPPIQARGIRIEIETLLSLPELLRKHQGDFARTGGIHA
                     AAVFDLHGHPQIVREDIGRHNAVDKVTGRAFLDGLLPLDRHLLLVSGRASFEIMQKAL
                     AAGIPIVASVSAPSTLAVSFARESKQTLIGFLRPPSFNIYSHIERVILEVRHPA"
BASE COUNT          649 a          781 c          753 g          612 t
ORIGIN      
        1 acatgtcagc aggttcgttg aagttccagt aacatcgttg ctcttcctcg ggcactcgga
       61 cggcccgcag cttgcctgtt ttcacgagtt gattcgttaa cgcctgcaac gaaaaatcat
      121 ttcccgaaag ggccgcggaa aatgtgtcgc aggcctcagc tggataaatt gcggccagcg
      181 gttcagccag atcgccaatc attggcaaga cgccgcaacc tcgctggatc tggctgcaca
      241 gaaaacgcaa gtgtccttcg ttcatcaatg gcatatcgat cgcgagagcg agcagatgac
      301 tgctttggat tcgagccaat gtcgcggtca acccaccgag tggtccgtgc gacggcggct
      361 cgtccggaac aaacgttgcg tctggcggtc gccagtccag atcagttcgc gctgaaacga
      421 agatttctgc cggctgcaat ttgcgcagga gcccgagttg aatttgccaa agcggttttc
      481 cgcgaaaaag gatgattgct ttatcgcggc ccattcgacg cgattgaccg ccggcaagca
      541 acactgcgct gatgttcatg ctccaataag atcgtcattc cgatcccgca gcgcgagaga
      601 ggaacctcac ataggctgat agatcacgct ctgtccctag cgtatcgcaa acttcgcagg
      661 tgaggtcctt cgctccgctc aggatgacag tttgcggcag tggagcgcag ttcgccatcg
      721 cagagaattt tggcgcgcaa gcctccacgt cctttgagaa attcttccgc gcccggggca
      781 atagcctgat tcatccaata acaagggctg cattcctgtg ttccgtgaaa atgaacgccc
      841 tggacttcaa agtcccggcc gatcagttca ttaagatcga caccgcgcgt catgacattg
      901 cgacggacta aggcgggcga acaaccgtgc aactcgagcg cgccgcaaag ttcatcaaaa
      961 acttcgaggg aaaagaatgt gatctgcccc ttgtaatcat ctttgaaatc gaaatagcga
     1021 tcactgcgaa tcccgcgacc agccacgcat tcgatcatcg gcacttcgac cgcaggataa
     1081 tcgcccggtt tgcgcccgtg atgaccgaca aaattgtggc cgggcgaaat gtaaagatgg
     1141 cagatttcca tcgccctatg ataacctcga gacgtgcatt tcacatcgga aaccggcgat
     1201 caaaatccct gtggctgccg atccataccc aaatccaaac gtcgccttca aatctttgcg
     1261 cgacggcccg ataatcgcgc gtcacacgca ctgaccagaa gcgcgaatcg gagcgcaacc
     1321 gctccagttg gagcgatgga tgcgctggat tccgctctag ttttctgtag gcgatgcgcg
     1381 cggcttcccg aaggttgtct ggcagagccc aataaagttt ccagaagtca gaagttgctc
     1441 tggacttcac gagcgctgct cgaaatcgct ctccttgatt tcaggaaacc ggttggcgtt
     1501 ggctttgaat tgagcttcga cttcatcgcc caaagccgac agtgcgggac gaggacgctt
     1561 atcgccgatg atatgctgcc acaccaggtc gccacgcaac tccggcagga tggaaggcaa
     1621 attttcagcc agccgttcgc gctcctccgg cggtaacgcc ttgatagccg cttcaatttc
     1681 agcgaccgtg ctcattgggc agacaagcta ttccggtggt gcaacgccgt aaaaccattt
     1741 ttcaccgccg gcaggtcgtt tcacatgaaa gaaaacggga tcaatacgca aaaaatcatc
     1801 cgtcgaaaag gggacggcga actggaatat caaccggacg atctcacgat cgaggagccg
     1861 ctcgagattc gcattgggcg taaggcgttg gcgacgacaa tgcgcacgcc gggccacgat
     1921 gaagaacttg cagctggttt tcttgtttcg gaagcaattg tccgcaagcc tggagaagtc
     1981 gcgaaaattt ccacgaatgg cgacaacatt gtgactgttg cgctcgcggc gggcgtgaga
     2041 ccgcagctca atttcgcgga acgcttcgga acgatttcat ccagttgtgg actgtgtggg
     2101 aaaaacagca tcgaagcgat ccggcaaaat tttcctccca ttcaagcgcg cggaatcagg
     2161 attgagatcg aaacgctgct gtcgcttccg gagctgttgc gaaaacacca gggtgatttt
     2221 gcgcgcacgg gtggaattca cgcagccgcg gtctttgatt tacacggaca cccgcaaatc
     2281 gtgcgcgaag acatcggccg ccataacgcc gtggacaaag taaccggccg cgcgtttttg
     2341 gatggccttt tgccattgga tcgacatctt ctcctggtta gtggacgcgc ctctttcgag
     2401 atcatgcaga aagcgttggc cgcgggtatt ccgatcgtgg cgtcagtttc cgcgccttct
     2461 acccttgcgg taagttttgc gcgcgagagc aaacaaacct tgataggttt tctccgcccg
     2521 ccctcgttca acatctactc gcacatcgag cgcgtgattt tggaagtcag gcatcctgcc
     2581 tgattcggcc cctcaggctt ccagcttcgc gatagggcga caagcaagtc tatcggcagt
     2641 cgcttttctc cgagcgtatg atccaaacga aatgatgcgc tgacttccgg tttcacatcg
     2701 tcatgctaag acggaacgct ttggcgaaaa ggcagcgatc gatcctgtcg aaggttcgcg
     2761 aaggaggctc ggtttcatcc gtcggtccaa aaatc
//
LOCUS       DASQUQ010000249         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_880625, whole genome shotgun sequence.
ACCESSION   DASQUQ010000249 DASQUQ010000000
VERSION     DASQUQ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880625"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VE486_06990"
     CDS             complement(<1..501)
                     /locus_tag="VE486_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38872.1"
                     /translation="MKILLAGFLGAVAMFIWTFIAHMALPLGEAGISEIPNEQAVLAA
                     MQSNIAEKSGLYFFPGLGVGPNATREEKNEAMKQEAAKMASGPSGLLIYNYARPFAFG
                     KALTIEFVTELVEAILVVFLLAQTAIASFAGRVGFVFLAGILAAIATNVSYANWYGFP
                     CLYTASY"
     gene            complement(563..1021)
                     /locus_tag="VE486_06995"
     CDS             complement(563..1021)
                     /locus_tag="VE486_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HZA38873.1"
                     /translation="MKTLSTSEAQKVSPPIEVRPSGVHGRGVFAAQFIPSDTRIIEYT
                     GKRVSWESAPNDENNPHTFNFGLENGEVINPEIGGNDARWINHCCDPNCEAIEEDDRI
                     FIYAIRDIEPGEELFYDYALEIDEPITEESKKEFACHCGSPDCRGTMLSL"
     gene            complement(1043..1414)
                     /locus_tag="VE486_07000"
     CDS             complement(1043..1414)
                     /locus_tag="VE486_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZA38874.1"
                     /translation="MTTESPAINYKIGNERLVSVTEKAARKLESLLEEKGKPNGALRL
                     AVVGGGCSGLQYVMDLVDGPKERDILVPTSNVRLVVDPKSALFVSGSVLDYSEDLQKG
                     GFKVTNPNAVAHCSCGESFAV"
     gene            complement(1429..1938)
                     /locus_tag="VE486_07005"
     CDS             complement(1429..1938)
                     /locus_tag="VE486_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38875.1"
                     /translation="MNPLPNEWAIKHRADACAVTHRPFEPGEYFYTLLFHDANGYRRE
                     DLSEDAWRNRNENIRPFSFWKSRYEPLPAPSAEPLPKENAEQLFRRLIASENPPANAS
                     YVLAAMLERKRVIKQIKAEENANGRVLIYEHGETGDVFIVPDPRLRLDELETVQNEVA
                     QLLHAETRD"
     gene            complement(1935..>2457)
                     /locus_tag="VE486_07010"
     CDS             complement(1935..>2457)
                     /locus_tag="VE486_07010"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZA38876.1"
                     /translation="AKVVHYDTAGNIVDEQIYPSLKVEHWSETPFRLGSANVRAEFLS
                     LPELREYLHFNSDFPATLLAPFRTHLQYRLALPWTCLVVVCIAAPLGIGYSRRGVLSS
                     VAAAVVLVFSMNFLVQLFLALGEGDRISPWIAAWTPNLLFTAIGLYLFYLRASNREAP
                     GFHLFTARRIVAR"
BASE COUNT          572 a          646 c          713 g          526 t
ORIGIN      
        1 gtagctggcg gtgtaaagac acgggaagcc ataccagttc gcatacgaaa cgttggtggc
       61 aatggcggcg agaattccgg ctaggaagac aaaacctact cggccggcaa agctcgcgat
      121 ggctgtctgg gcaaggagga acacgacgag gatcgcttcg accagttcgg tgacaaattc
      181 gatcgtcaac gcctttccga atgcgaacgg ccgcgcgtaa ttgtaaatca ggagacccga
      241 tgggccgcta gccatcttcg ctgcctcttg tttcatcgct tcgttctttt cctcccgcgt
      301 tgcgttcggc ccgacaccga gccccggaaa gaagtaaaga cccgactttt ctgcgatgtt
      361 cgattgcatc gcggcgagga cagcttgctc attggggatt tcgctgatgc cagcttcgcc
      421 cagaggcaac gccatgtggg cgatgaatgt ccagataaac atggcgacag cgccaagaaa
      481 accagcgaga aggattttca tagacaggaa gactgaagga taaaacagga atttagaagg
      541 aaaaaatcag gcgcggctgg agctacagcg aaagcattgt gccgcggcag tctggactac
      601 cgcagtggca cgcgaattcc ttctttgatt cttcggtgat gggttcgtcg atttccagcg
      661 cataatcgta aaagagttcc tcaccgggtt cgatatcgcg tattgcgtag atgaaaatgc
      721 ggtcgtcttc ctcgatcgct tcgcagttcg gatcgcagca atgattgatc cagcgcgcgt
      781 cgttgccgcc gatctcggga ttgattactt cgccgttttc gaggccgaaa ttaaatgtgt
      841 gcggattgtt ctcgtcattg ggagcgctct cccaggagac gcgtttcccg gtgtattcga
      901 tgatccgggt atcgctcggg atgaactgcg cggcgaaaac gccgcggccg tggacaccag
      961 aagggcggac ctcgataggc ggtgagactt tttgagcctc ggaagttgag agggtcttca
     1021 agcgacgaac gcgcgaataa gctcaaacgg cgaaactttc accgcaggaa cagtgggcga
     1081 cggcgtttgg gttggttact ttgaagccgc ctttctgcaa atcttcgctg taatcaagga
     1141 ccgagccgct gacgaatagc gcgctctttg gatcgacaac caggcgcacg ttggaggtcg
     1201 gaacgaggat gtcgcgctct ttggggccat ccaccaaatc catgacgtat tgcaggcccg
     1261 agcagccacc gccaactacg gcgaggcgca gcgcgccatt tggcttacct ttttcctcca
     1321 acagcgattc gagtttgcgc gcggccttct cggtcacgct gaccaggcgc tcgttgccga
     1381 tcttataatt gattgccggg gattcggtcg tcattcaaaa ggtgatcttc aatctcgcgt
     1441 ttctgcatgg agcaattggg cgacttcatt ttgcacggtt tccaattcgt cgagccgcaa
     1501 tcgtgggtcc ggcacgatga agacgtcgcc cgtctcgcca tgttcgtaaa tcaaaacccg
     1561 accgttcgca ttttcctcgg ccttaatttg tttgattact cgcttgcgct caagcatggc
     1621 agccaaaacg taggaggcat ttgccggagg gttttctgat gcaatcaaac gacggaaaag
     1681 ctgctcggca ttctcttttg gaagcggttc agccgatggc gccggcagag gctcatagcg
     1741 cgatttccaa aatgaaaacg gccggatatt ctcgttccga ttacgccagg cgtcttcgct
     1801 caaatcttcg cgccgatatc cgttggcgtc gtgaaaaagc agggtataga aatattcccc
     1861 cggttcgaac ggtcgatggg tcacggcgca ggcatccgcc cgatgcttga tagcccattc
     1921 gtttgggagc ggattcatcg cgcaactata cgtcgcgcgg tgaaaagatg aaagccgggc
     1981 gcttcgcgat tcgaagcgcg aagataaaac agatagaggc caatcgcggt gaaaaggaga
     2041 ttgggcgtcc acgcagcgat ccagggagag atgcggtcgc cttcgccgag cgcgaggaaa
     2101 agctggacca ggaagttcat tgagaaaacg agaactaccg cagccgcaac actcgacagt
     2161 acgccccgcc gcgagtaccc gattccgagc ggggccgcga tacaaacgac caccaggcac
     2221 gtccacggta aagcgagacg atactgaaga tgagtgcgga acggcgcgag cagagtagcg
     2281 ggaaaatccg aattgaaatg cagatactcc cgtagctcgg gcaagctaag gaattccgcg
     2341 cgcacgtttg cgctgcccaa ccggaagggg gtttcgctcc agtgctcgac cttgagcgaa
     2401 ggataaatct gttcgtcaac gatatttcca gcggtgtcgt aatgaacaac tttcgcg
//
LOCUS       DASQUQ010000250         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_432343, whole genome shotgun sequence.
ACCESSION   DASQUQ010000250 DASQUQ010000000
VERSION     DASQUQ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432343"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2664)
                     /locus_tag="VE486_07015"
     CDS             complement(<1..>2664)
                     /locus_tag="VE486_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZA38877.1"
                     /translation="ILVGDQVLSYQELAQYFNAKKNVSLPQIFIRVNKDADEVQLERS
                     RTRESSLRVTFGLKVRVKALRDVDAELNGVKLKAGAKVEATLEDKIVFHNDSEMDLSD
                     LRRRARALGGRFQLKASKSEYLVSNEPSRLEADDILLSPGTSGDVLLKIFCDYDQRVG
                     LLEVIEADRPILVGDTPVRTTAPLKDGDTIRIDIGQFLRCNFSERIVEEERNIIRTLD
                     VNEITHRFSKGGIGLDGISFSISRGELVCVMGASGSGKSTLLKVLAGQLRPTSGEVIL
                     NGQSLYSNLDVLKRYVSYMPQQDAFDEHLTIGENLQFAAAIRAPHLSRRDRTRRLEAK
                     LIELGLGERRDAVVGSPESKTLSGGERKRLNVGLDMIGMSDVYLFDEPTSGLSSKDSE
                     HVIEIIRGMAHNKIIIVTIHQPSSKIFQMFHKAILLDKGGRLVFFGTPSDMLRYFAEA
                     EHQHQFGAELGACPSCGTTRPEFIFDVLETPLRDISGDIIYEENSRGQLVAARRYSPE
                     FWRDKYEAFRLIQDVKQVSLRHEPAAPLPAAPAEKKRLPIRWHDEWTQFRTLLRRAFI
                     SKLRNRANLVITIGVSPVLALLIAILLRYSESGKYDFASAYHIPTFLFLGLIVAMFLG
                     LTNSADDIIRDRAVLQRERNLSVRLSYYVFSKTLTLGIFALIQCVLFVLIGNYILQIR
                     GMFWIYLGIMLMTAMGGVALGLLISSLVADPKTAANIVPLVLIPQIIMGGALIKYEDM
                     NRNLALVYTLSHWFSEHPSADQSKKMESKLEVPFVCQFIAMRWSYEEIILAQAKLNPL
                     ARRQDRASNEIQRLAHEANTPEQRKRLDDLKEVLALLSGLEGASAKEIDRYLKLIDPV
                     LAGKQKFDRSLFRNANGPVTAEQIYVNQKVS"
BASE COUNT          600 a          730 c          728 g          606 t
ORIGIN      
        1 tcggacacct tctgatttac ataaatctgt tcggccgtga ccggaccatt tgcgttccga
       61 aaaagcgagc gatcgaactt ttgtttcccg gcaaggacag gatcgatcag tttgagataa
      121 cgatcgatct ccttcgccga agctccttcg agaccggaca gcaatgccaa aacttccttc
      181 aggtcatcca gacgcttgcg ttgttcaggt gtgttcgctt cgtgggcgag cctttgaatt
      241 tcgttgctgg ctcgatcttg ccggcgcgcc agcgggttga gcttggcttg cgcgagaatg
      301 atctcttcgt acgaccaacg catggcgata aattgacaaa caaatggaac ctccagcttg
      361 ctctccatct tcttgctttg gtcggcgctg ggatgttcgg agaaccaatg cgaaagcgta
      421 taaacaagag cgaggttccg gttcatgtcc tcatacttga tgagcgcgcc gcccatgatg
      481 atctgcggaa tcaagaccag cgggacgatg ttcgccgccg ttttcggatc ggcaacgagc
      541 gaagaaatga gcagcccaag cgccacgccg cccattgccg tcatgagcat gatgccaagg
      601 taaatccaaa acatcccgcg gatttgcaga atgtaattcc cgatcaggac aaaaagcacg
      661 cactggatca gagcaaatat tccgagagtg agggtttttg agaagacgta gtacgacagg
      721 cgaacgctga gattgcgttc ccgttggagc acagcgcggt cgcgaatgat atcgtcggca
      781 ctgttggtta gcccaagaaa catcgcaacg atcaggccca aaaacaaaaa cgtcggaatg
      841 tgataagccg acgcgaaatc gtatttgccg ctttctgagt agcgcaacag gatcgcgatg
      901 agcagcgcca gcacgggcga gacgccgatg gtgatgacga ggttggcgcg attgcgcagt
      961 ttgcttatga atgcgcgccg caacagcgtg cggaattgtg tccactcgtc gtgccagcga
     1021 attggcagcc ttttcttttc ggcgggcgcc gcgggcagcg gcgcagctgg ttcgtggcgc
     1081 agggaaactt gtttgacgtc ctggatcaat cggaacgctt cgtacttatc gcgccagaat
     1141 tccggtgaat aacggcgcgc ggcgaccaac tggccacgac tgttttcctc gtaaatgatg
     1201 tcgccgctta tatcgcggag cggtgtctcg aggacgtcaa aaatgaactc ggggcgtgtc
     1261 gttccacagg aaggacaggc gcccagctcg gcgccgaact gatgctggtg ttccgcttca
     1321 gcaaaatagc gaagcatgtc tgagggcgtg ccgaaaaata cgagcctgcc tcctttgtcg
     1381 aggaggatcg ccttatggaa catctgaaag attttggagc tcggctggtg aatggtgaca
     1441 atgatgatct tgttgtgcgc cattccgcgt atgatctcta tgacatgctc ggagtccttc
     1501 gaggagaggc cggaagtcgg ctcatcgaaa aggtaaacat ctgacatacc gatcatatct
     1561 aggccaacgt tgaggcgttt ccgctcgccg ccgctcaatg tcttgctctc cgggctgccc
     1621 accacggcgt cccggcgttc gcccaggccg agctcgatca gttttgcctc cagacgccgg
     1681 gtccgatcgc gtcgcgacaa gtgcggcgcc cggatggccg ccgcaaactg caggttttcg
     1741 ccgattgtga gatgctcgtc gaaagcgtcc tgttgcggca tgtaactgac gtagcgcttg
     1801 agaacgtcga ggttcgaata aagcgattgt ccgttgagga taacttcgcc gctagtaggc
     1861 cggagttgcc cggccagcac ctttaacagc gtgctttttc ccgaaccact cgcacccatc
     1921 acgcaaacga gttcgccccg actgatactg aaagaaattc catcaagtcc gattccgccc
     1981 ttgctaaacc gatgggtgat ctcgttgaca tcgagcgtcc gaatgatgtt gcgctcttcc
     2041 tcgacaattc gttccgaaaa attgcaccgc agaaattgtc ctatgtcgat tcgaatggta
     2101 tcgccgtctt tgagcggcgc ggttgtgcgc acgggagtat cccctaccag gattggacga
     2161 tcggcttcaa ttacctccag aagacctacc cgttgatcgt aatcgcaaaa aatcttcagc
     2221 agcacgtcac cgctggtgcc gggagaaagt agaatatcgt cggcttcaag ccggctgggc
     2281 tcgttggaga ccaagtattc ggatttggag gcctttaatt gaaaccggcc gcccaatgcg
     2341 cgtgcccgtc ggcgcagatc gcttaaatcc atctcgctat cgttatggaa gacgatcttg
     2401 tcttccaagg tagcctccac cttcgcgccg gccttcaatt tcactccatt gagctcggca
     2461 tcgacatcgc gcaacgcctt cacgcgcacc tttaagccga aagtaacccg gagcgagctt
     2521 tcgcgcgtcc gcgagcgctc cagttgcact tcatccgcgt ccttatttac tcggatgaaa
     2581 atctgtggaa gcgaaacatt cttttttgca ttaaaatatt gcgccagctc ctgatagctc
     2641 agcacctgat cgccaaccaa aatg
//
LOCUS       DASQUQ010000251         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_161072, whole genome shotgun sequence.
ACCESSION   DASQUQ010000251 DASQUQ010000000
VERSION     DASQUQ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161072"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VE486_07020"
     CDS             complement(<1..1211)
                     /locus_tag="VE486_07020"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZA38878.1"
                     /translation="MRLVVQKYGGTSVGTPERIRNVARRLVETQREGAHVVAVISAIA
                     GVTDQLIKLAHEISPHPTEREFDILLATGEHAATALTAMAINALGGKAISLTGAQAGI
                     LTDRHHTKARIANISPRQIHELLSDDYIVIVAGFQGQNPQGETTTLGRGGSDLTAIAL
                     AGALNADACQIFTDVDGVFTCDPRVVTDAKKLDEVAYDELLEMASAGSKVMQSRAVEF
                     AKKFGIEFEVRSSLKTSPGTIAKEETPSMEDVVIRGISLERHQAKLTVAGVRDEPGNA
                     GRIFSNIAAAHIIVDMIVQNASIGGTTDVSFTIHEDELENARTILMPVVSEVGAKRLN
                     TVSGVAKLSVVGIGMRSHSGVAARMFESLGRGGINIQLVSTSEIKIAVIIDEKDAERA
                     AQLIHAEFGLAR"
     gene            complement(1331..2422)
                     /gene="thrC"
                     /locus_tag="VE486_07025"
     CDS             complement(1331..2422)
                     /gene="thrC"
                     /locus_tag="VE486_07025"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZA38879.1"
                     /translation="MTSGPERGRGVIERYREYLPVSESTKIISLGEGDTPLIYCPELS
                     KRVGRGCHVFVKNEGVNPTGSFKDRGMTVAVSTAMERGARAIICASTGNTSASAAAYA
                     ARAGISCAVILPAGKIATGKLLQAFAYGAKIVAVDGNFDDALRIVRELGVEGDFVIVN
                     SINPDRIAGQKTAAFEIVDELGEPPDFHLLPVGNAGNITAYWAGYREYHTHQRSRRLP
                     VMIGCQAAGAAPIFHNRIIEQPETIASAMRIGNPASWKQARAAIEESRGAVDVVTDEE
                     ILAAQMWLARHEGIFVEPASAAAIAGFFKCCDSNEAVYSFREISEGRRIVCTVTGHGL
                     KDPDVIMQRMRELKSVPATVDDVRRAIDL"
     gene            2503..>2806
                     /locus_tag="VE486_07030"
     CDS             2503..>2806
                     /locus_tag="VE486_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38880.1"
                     /translation="MGETLRQDEAAMEFCFRVRGHAEKPLQTVPESLSFSNPAAAGLI
                     RIRSHRMASLDKETKFRLLKTILESRHADLREQNLNRQGKGHFHVSGMGHEALAAIS"
BASE COUNT          594 a          833 c          731 g          648 t
ORIGIN      
        1 cgggccaagc caaactcggc gtgtattagt tgggccgcgc gttccgcgtc tttttcgtca
       61 ataatgactg cgattttgat ctcggaggtt gagaccaact ggatgttgat cccgccgcgc
      121 cccagggact cgaacattcg cgcggctacg ccggaatgcg atcgcatccc gatgccgacg
      181 acgctcagtt tagccacgcc gcttactgtg ttcaagcgct tggcgccgac ttcgctgacg
      241 acgggcatca aaattgtccg agcattttcc aactcatcct cgtgaatcgt aaatgaaacg
      301 tcggtcgttc cgccgatcga cgcattttgc acgatcatgt ccacgatgat gtgcgcggcg
      361 gctatgttcg agaaaattcg accggcgttt cccggttcgt cccgcactcc tgcgaccgtg
      421 agtttggcct gatggcgctc gagactgatt ccacggatca ctacgtcctc catgctcggc
      481 gtctcttctt tcgcgattgt tccgggactg gttttcagac ttgaccgcac ctcgaattcg
      541 atcccaaatt tctttgcaaa ctccaccgcg cgcgattgca tcaccttcga gcctgcgctc
      601 gccatctcga gcaactcatc gtaggcgacc tcgtcgagtt tttttgcgtc agtcactacg
      661 cgcggatcgc acgtgaagac tccatcgaca tcggtaaaga tctgacaggc gtcagcattc
      721 aaagcgcccg ccagcgcaat tgccgttaag tcggacccgc cccggccgag cgtcgtcgtt
      781 tctccttgag gattctgacc ctgaaaaccg gcgacgatga caatataatc atccgacagc
      841 agttcatgaa tctgtcttgg actgatgttt gcgatccgcg ccttggtgtg atggcgatcg
      901 gttaaaattc ccgcctgcgc gcccgtaagc gagatggctt ttccgcccag tgcgttaatg
      961 gccatggcag tcaacgccgt cgcggcatgt tcgccagttg cgagcaaaat atcgaactca
     1021 cgctcggttg ggtgcggcga tatctcgtgc gccagcttga tcaactggtc cgtcacgcct
     1081 gcgattgccg agatcaccgc gacgacatga gcaccttcgc gttgcgtttc gacgagacga
     1141 cgcgccacgt tacggattcg ctccggtgtt ccgacggaag tgccaccgta tttttgaaca
     1201 accaggcgca tgtcaggagt ccattatggc gcaaccgttt gtcattccga gcggagtcga
     1261 ggaatctctc actattacac agtaatcccg cgatgccgtt tcgaacttcg cccgcaaaga
     1321 tagatagctt ttagagatca atcgcgcgcc gaacatcgtc cacggtcgcg gggacggatt
     1381 tcaattctct catgcgctgc atgattacat cggggtcttt cagtccgtgc ccggtaacag
     1441 tgcaaacaat ccgcctccct tccgaaattt ctctgaatga atacacagct tcgtttgaat
     1501 cgcagcattt aaaaaatccg gcgatggccg ctgcgctggc cggttcgaca aaaattcctt
     1561 cgtgtcgcgc cagccacatc tgcgccgcta gaatttcctc atcagtgaca acgtcaactg
     1621 cgccgcgcga ttcctcgatt gccgctcgcg cttgtttcca actggctgga ttaccaattc
     1681 gcatggccga cgcgatcgtc tcaggttgct cgatgatccg gttgtgaaaa atcggagctg
     1741 ccccggcggc ttgacaacca atcatcaccg gtagccttct cgatcgttgg tgtgtgtgat
     1801 attcgcgata gccagcccaa tatgcggtga tatttccggc gttgcctacc ggcagcaaat
     1861 gaaaatcagg cggctcccca agttcatcga caatctcaaa cgcggcggtc ttttgaccag
     1921 caattcggtc cgggttaatc gaattcacga tgacgaaatc gccttccact ccgagctcgc
     1981 gcacaatccg aagcgcatcg tcaaagttcc cgtcaacagc cacgattttt gcgccataag
     2041 caaaagcctg cagcaatttg ccggtcgcaa tctttccggc aggcaaaatt accgcgcagg
     2101 agattcccgc gcgcgctgcg taagcagccg cggaagcgga agtatttcca gttgaagcac
     2161 agatgattgc gcgcgcgccg cgttccatcg ctgtggacac agcgaccgtc atgccgcgat
     2221 ccttgaacga gcccgtggga ttaaccccct cgtttttcac aaaaacatgg cagccgcggc
     2281 cgacgcgctt gctaagttcg gggcaataga tcagcggcgt atccccctcg cccagggaaa
     2341 taatcttcgt cgattcggaa accggcagat actcgcggta acgctcgatc actcccctgc
     2401 cccgctctgg accagatgtc ataaacacaa accgtcgtag ccacggcgct gctcgccacg
     2461 ccggagcatt gcgaaggcgg gtgccgccgt gttttgggag cagtgggcga aacgcttcga
     2521 caggacgagg cggctatgga attctgcttt cgagttcgtg gacatgcgga aaagcctttg
     2581 caaacggtgc ctgaatctct ttcctttagc aatccggcgg ctgccggact tattcgtata
     2641 agatcgcatc gtatggcttc gctggacaaa gaaaccaagt ttcgtcttct caaaacgatc
     2701 ctggaaagcc gccatgcaga tctgcgtgaa cagaatttga atcgtcaggg caaaggacac
     2761 tttcacgttt ccggaatggg ccacgaggcg ctggctgcaa tttcat
//
LOCUS       DASQUQ010000252         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1348359, whole genome shotgun sequence.
ACCESSION   DASQUQ010000252 DASQUQ010000000
VERSION     DASQUQ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348359"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VE486_07035"
     CDS             <1..380
                     /locus_tag="VE486_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HZA38881.1"
                     /translation="TGVFVVETKTKRKPLLGGEKQYRVVFDGQKLHFPNGWDTDALDQ
                     VRRNKKTLCQWLSSATGDHVTAEGIVTIPGWLVERIAKSNVYMVNPKEIRSLVVSLSG
                     RPLTSAEIQRISHQLEQKCKLAV"
     gene            complement(424..639)
                     /locus_tag="VE486_07040"
     CDS             complement(424..639)
                     /locus_tag="VE486_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38882.1"
                     /translation="MFELTIGCLKWAVVGFVVAFTLMKLYEASHIHDDIPRPEFKVRQ
                     SKRAALILSMFAGMLSGLVWLLVQKLD"
     gene            743..>3821
                     /gene="mfd"
                     /locus_tag="VE486_07045"
     CDS             743..>3821
                     /gene="mfd"
                     /locus_tag="VE486_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZA38883.1"
                     /translation="MVEKSGRKELLEIVASSEPIARKLRAIDRAQWPVQFSHVTQPAQ
                     AFLVATIAHRIDRTIWVVCPNVHSQELVYESLLNWHPAALFLPEAELAAVENVLPDPE
                     IAAERLALLTQVEREGGWHLIVATRASLDQAAPTRDVLRSAIVQLRRGANAQMEHLIE
                     QLAGAGYERVAQVTTRGQFAVRGGIVDLYSWQALLPARLEFFGDEIDSLREFDIDTQT
                     SVRDLRSVDILLEATEGQSGVVRDYVGRDHLTIELEPATEGGAPATRGSRELAPPQIK
                     ISEGWIEIGPEDFSGAFEDCEAGEFAIGDLMLVEAKRAQFSTRLEGWRANKARIVIYF
                     QTEGEIERFREVMGETQALDDVDLLEGTLAHGFCFPAANLVVLSAAELFGRLAAHPRR
                     RLHRAERHRAQIDFSELNEGDLIVHLEHGVGRFLGLKKFAVGQALRLPGADEKDSASE
                     ALALQRQPQEVLVLEFANEAKLYVPLEQAYLVSRYVGVGKRSPALSSLGDAKWARAKK
                     NATASIFDYAGKMLAVQAERQMHPGHAFAPDGKWQAEFERSFPFRETPDQLRAIIDTK
                     IDMERRQPMDRLICGDVGFGKTEVAIRAAFKAIIDGKQVAVLAPTTVLAQQHFEVFRQ
                     RMLDYPVRIEMLSRFRSQGEQKKILELLRKGGVDIVIGTHRLISGDVAFKDLGLVVID
                     EEQRFGVLHKEKFKELFKLVDVLTLSATPIPRTLYLSLVGVKDMSTIETPPLNRLPVE
                     TMVSAYDERIIRDAINRELERQGQVFFLHNRVQTIERARDRIMELCPQARVEIGHGQM
                     ESDELEAVMARFIAGEVDVLVCTTIIESGLDIPNANTIIIDRADRFGLADLYQLRGRV
                     GRAEHKAYAYLLLPREMMTIGAARKRINAIKQYSSLGAGFRIAMRDLEIRGAGSILGT
                     AQSGHIMTVGFDLYCQLLKQAVAQLKGDKVQPRLDVDLRLDFVATNEAEFVQLGPEVR
                     VPAFVPGNFISDAGLRIKAYRDFAQMGTHEQLEHLRRDWRDRFGRFPASVDNLF"
BASE COUNT          836 a         1053 c         1108 g          824 t
ORIGIN      
        1 caaccggcgt gttcgtcgtc gaaacaaaaa ccaagaggaa gccgctgctc ggaggcgaga
       61 agcagtaccg agtcgtattc gatggccaga agctgcactt cccgaatggt tgggacaccg
      121 atgctttgga ccaagtaagg cgaaacaaaa aaacgctctg ccaatggctt agttccgcaa
      181 ctggcgacca cgttactgct gagggaatcg tgactattcc tggctggttg gttgagcgaa
      241 ttgccaagtc caacgtctac atggtaaacc cgaaagagat tcgaagttta gttgtgagct
      301 tgtctgggcg tccgctgacc agcgccgaaa tccagcgcat ttcccatcaa cttgagcaga
      361 agtgcaagct ggcggtctga ctgctgatct ctgatttctg atctgtcgct tctgattcga
      421 agctcagtcc aatttctgca ccaacagcca caccagtccg gacagcatcc ccgcgaacat
      481 ggacaggatg agcgctgcgc gtttcgactg gcgtactttg aactcaggcc tgggaatatc
      541 gtcgtgaata tgactggcct catagagctt catcaaggtg aacgccacca cgaagccgac
      601 aacggcccac ttgagacacc caatggtcag ttcaaacatt tcggtcaaac tctaccgcga
      661 gaactgcggc gctcaacaag cggctgcgat gcctttttgc cggggtaatt ttgaacttcc
      721 accgcttttc gacgacagct aaatggtgga gaaatcgggg cggaaagagc ttctggagat
      781 cgttgcgtcg agcgagccaa tcgcgcgcaa actgcgcgca atcgatcgag cgcaatggcc
      841 ggtccagttc tcgcacgtga cccagccggc gcaggcgttt ctcgtcgcca ccatcgccca
      901 tcggatcgac cggacaattt gggtcgtctg tcccaacgtt cattctcaag aactggttta
      961 tgagagcttg ttgaactggc atccggccgc gcttttcctg ccggaagccg agctcgccgc
     1021 cgtcgaaaat gttttgcccg atccagaaat cgcagcggaa cggctcgcgc tgcttacaca
     1081 ggtcgagcgc gagggcggtt ggcatctgat cgtcgcaacg cgcgcgagtc tcgatcaagc
     1141 cgcgccaacg cgcgatgtac tccgatccgc aatcgttcaa ctgcggcgcg gggcaaacgc
     1201 gcagatggaa cacctcatcg aacaacttgc tggcgctggt tatgagcgcg tcgctcaagt
     1261 tactacccgc gggcaattcg ccgtccgcgg aggcattgtc gatctctatt cgtggcaggc
     1321 gctcttgccg gcgcgcctgg aattttttgg cgacgaaatc gattcgttac gcgagttcga
     1381 catcgatact caaacctcgg tgcgcgattt gaggtcggtc gatattctgc tggaagcgac
     1441 tgaaggtcaa agcggcgtgg tccgcgatta cgtcgggcgc gatcatctca cgatcgagct
     1501 ggagccagcc acggagggcg gagctccagc gacgcggggc tcgcgggagc tcgcccctcc
     1561 gcaaatcaaa atcagcgaag gctggattga gatcggtccg gaagatttta gcggcgcgtt
     1621 cgaggattgt gaagccggcg agttcgcgat tggtgatctc atgctcgtcg aagctaaacg
     1681 cgctcaattt tccacgcgcc tggagggttg gcgcgcgaac aaggccagga tcgttatcta
     1741 cttccagact gaaggcgaaa tcgaacgttt ccgcgaggtc atgggcgaaa cgcaggcgct
     1801 ggatgatgtc gatcttctcg aagggactct cgcgcatgga ttttgttttc cggcggccaa
     1861 tctcgtcgtg ctctcggctg ctgaattatt cggacgattg gctgcccatc cacgccgacg
     1921 cctgcatcgt gcggaacggc atcgcgccca gattgatttc agcgaactca acgaaggcga
     1981 cctgatcgtt catttggagc acggcgtcgg gcgctttctg gggcttaaaa aatttgctgt
     2041 aggacaggcg cttcgcctgc caggcgcgga cgaaaaagac tcggcaagcg aagcgcttgc
     2101 cctacaacgg caaccgcagg aggtgctggt tctcgagttt gccaatgaag cgaaactcta
     2161 cgttccactc gaacaagctt atttggtttc gcgttacgtg ggcgtcggca aacggtcacc
     2221 ggctctcagt tcgctaggcg atgcgaaatg ggcgcgcgcg aagaaaaacg ccacagcttc
     2281 gattttcgac tacgccggca aaatgctcgc ggtccaggcg gagcggcaga tgcatcccgg
     2341 ccacgccttt gctcctgatg gcaaatggca ggcggaattc gaacgttcat ttccgtttcg
     2401 cgaaacgccg gaccaattga gagcaatcat cgacacgaaa atcgatatgg agcgccggca
     2461 gccgatggac cgtttgattt gtggtgatgt cggctttggc aagaccgaag tcgcaattcg
     2521 cgctgcattt aaagcgatca ttgacggcaa acaagttgcc gtcctcgcgc cgacgaccgt
     2581 tctggcccag cagcatttcg aggtgtttcg gcaacggatg cttgattatc ccgtccgaat
     2641 cgaaatgctc agccgtttcc ggtcgcaagg agagcagaaa aaaatcctcg aacttttgcg
     2701 caaaggcggc gtcgatatcg tcatcggcac gcatcggctc atctcaggcg atgtcgcttt
     2761 taaagatctc gggctggtgg tgatcgacga agaacagcgg tttggagttc tgcacaaaga
     2821 aaaattcaaa gagttgttta agctcgtcga tgtcttaacg ctctccgcca cacccattcc
     2881 ccgcaccctt tacttgtcgc tggtgggtgt gaaagacatg tccacgatcg aaacgccgcc
     2941 actcaaccgg ttgccggtcg agacgatggt ttccgcctat gacgagcgaa ttattcgcga
     3001 tgccatcaat cgcgagctgg agcgccaggg gcaggttttc tttttgcaca atcgcgtcca
     3061 aaccatcgag cgcgcgcgcg accgcatcat ggaactttgc cctcaggcgc gggttgaaat
     3121 cgggcatggc cagatggagt cggatgaact cgaagcggtc atggcgcgat tcattgccgg
     3181 agaggtggat gtgctcgttt gcaccacgat tatcgaaagc gggctcgaca ttccgaacgc
     3241 caacaccatc atcatcgatc gcgcggaccg gtttggtctt gctgacctct atcaattgcg
     3301 cggacgcgtc ggccgggccg agcataaagc gtatgcctat ctgcttcttc cccgcgaaat
     3361 gatgacaatc ggagctgcgc gcaagcggat caatgccatc aagcagtaca gttcgctggg
     3421 cgcgggtttt cgcattgcga tgcgcgactt ggagattcgg ggcgcgggca gcatccttgg
     3481 gacagcgcaa agcggccaca ttatgacggt gggcttcgat ttatattgtc aattgctcaa
     3541 gcaagcagta gcgcaactta aaggcgataa agtgcagccc cggctggatg tcgacctgcg
     3601 tctcgatttt gtcgctacaa acgaagcgga gtttgttcag cttgggccgg aggtgcgcgt
     3661 gcccgccttt gtgcccggca attttatcag cgacgccggc ctgcgaatca aagcctatcg
     3721 tgacttcgcg caaatgggga cgcatgaaca attggaacac ctgcgacgcg attggcgcga
     3781 ccggtttgga cgatttccag cctcggtgga caatttgttt c
//
LOCUS       DASQUQ010000253         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_796566, whole genome shotgun sequence.
ACCESSION   DASQUQ010000253 DASQUQ010000000
VERSION     DASQUQ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796566"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VE486_07050"
     CDS             <1..409
                     /locus_tag="VE486_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38884.1"
                     /translation="FIDNLPYPIFHPEREQARFGSLCVDEIDARKKRSGFESLSDMDH
                     SSAEALAEFPERGLNWAKEQFETVLNETEDYVREKPAESLLCALAVGYILNRLPVGRI
                     LGGLFRLLIVALRPAILIYGATKLYQAVREEES"
     gene            complement(472..684)
                     /locus_tag="VE486_07055"
     CDS             complement(472..684)
                     /locus_tag="VE486_07055"
                     /inference="COORDINATES: protein motif:HMM:NF043052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HZA38885.1"
                     /translation="MSEHIYKKIELVGSSPNGFEEAVKNALARAEKTMRNMRWFEVAE
                     TRGYIENGKVGHWQVTLKVGFTLEDA"
     gene            840..1355
                     /locus_tag="VE486_07060"
     CDS             840..1355
                     /locus_tag="VE486_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38886.1"
                     /translation="MGKSFAACLVFLVALSCARAQDQESKLIDRLLRPDTTLRNNAQN
                     KQFIADGASINKKATVGTFYVQKKSNSKSFAGTRDFSARQFNSQSFQGTRSASGISSQ
                     QAIGNSRPAYATRTARGPRDAPQSAKKVASGSFAGNRPFLDQGKSQKSLNRQNAPLTI
                     EQVRELLNKNK"
     gene            1387..2565
                     /gene="tyrS"
                     /locus_tag="VE486_07065"
     CDS             1387..2565
                     /gene="tyrS"
                     /locus_tag="VE486_07065"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZA38887.1"
                     /translation="MMQPDEALALLKHGAAQIISEDELREKLALGRPLRVKLGVDPTT
                     RDIHLGHSIVLRKLRQFQDLGHQAVLIIGDFTGMIGDPSGRSATRPQLTHKEILANAA
                     TYREQAFKILDPAHTETVCNGDWFRAMSFEDVIRLNSRVTLQQMLQREDFRARIDNQQ
                     AIHAHEIQYPIMQGWDSVMVKADVELGGTDQLFNILVGRDFQKEEGLPQQVVFLLPIL
                     EGLDGSKKMSKTLGNFVAINEPATDVFGKLMSISDELMARYYPLLLSRSLASEAHPLE
                     AKKQLAFEIVQTYHSTEVARKTLDEWNTRFSRRELEHANLPIFPGAALQNLEAVTLIS
                     KIYQEAFGLQKSRSETSRLIKQGSVEVDGVKIRDPKATTTLKAGQVLRLDKTHMVRIG
                     "
     gene            complement(2632..3015)
                     /locus_tag="VE486_07070"
     CDS             complement(2632..3015)
                     /locus_tag="VE486_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38888.1"
                     /translation="MVTRATVTSSKTVDFSFRGPLGFEAHTVLLSVESDSPDQYAFES
                     VGGNIDLMGIVDFTEIRPNCTEVTLAVHYEIKNKLFAWLDRRLRFVDGFVTAELRSIR
                     AHFEGIAASYLEHARVMPVLQTAAA"
     gene            complement(3235..3885)
                     /gene="bioD"
                     /locus_tag="VE486_07075"
     CDS             complement(3235..3885)
                     /gene="bioD"
                     /locus_tag="VE486_07075"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HZA38889.1"
                     /translation="MSLFITGTDTGVGKTHTAVQLLRLLRAAGISCAGMKPICCGDRR
                     DAELLLAAGSDGLTIDEVNPVWLETPAAPIVGSLMEEVKIDIVQILAAFRALQGRVEH
                     VIVEGVGGWLVPIRSDYFVSDLAVEMQSPVLVVAQNRLGCLNHTALTLRSVAQHKLRC
                     VGVALNDVPVSDDIAALTNADILGKIVNVPLLGGLGKTLMELPADWRLMIESTRKP"
     gene            complement(3882..>4536)
                     /gene="purQ"
                     /locus_tag="VE486_07080"
     CDS             complement(3882..>4536)
                     /gene="purQ"
                     /locus_tag="VE486_07080"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZA38890.1"
                     /translation="RAEYVWHKETSLDGFDAIVLPGGFAYGDYLRCGAIARFSPIMRA
                     VISDARAGKLVLGTCNGFQVLCEAGLLPGALVRNRSLRFVCDMVTMRVEVDDSPFTHG
                     CPKGSPLRMPVAHGEGCFFADPETLRDLNANQQVILRYSDDDGRIVAEANPNGSIENI
                     AGICNREGNVFGLMPHPDRASDARLGSADGAKIFRSMMQTISASRVTRASERVKQVA"
BASE COUNT         1090 a         1288 c         1176 g          982 t
ORIGIN      
        1 tttcatcgac aacctcccct acccgatttt ccacccggaa cgggagcaag ctcgatttgg
       61 ctcgctttgc gttgacgaga tcgatgcgcg caaaaaacga tcaggattcg aatcattaag
      121 cgacatggat cattcatctg ctgaagcgtt ggctgagttc ccggaacggg ggttgaactg
      181 ggcgaaagaa cagttcgaga cggttcttaa cgagactgag gattatgtgc gcgaaaaacc
      241 agcagagtct ctgctttgcg cgctggcagt gggatacatt ctgaatcgat tgccggttgg
      301 ccgaattctg ggcggtctct ttcgcctcct tatcgtcgcg ttgagacccg cgatcttaat
      361 ttacggcgcc accaagctct accaggcggt ccgggaagaa gaatcttaat cgttaaaatc
      421 gctaatcggt tcgacatcgc gaacgatgca ccctctttag catttttggg ttcaagcgtc
      481 ctcgagcgta aagccgacct tcagcgtcac ctgccaatga ccaaccttcc cgttctcgat
      541 gtatccgcgg gtctccgcca cttcgaacca gcgcatattc cgcatcgttt tttcggcgcg
      601 ggccagcgcg ttttttactg cctcctcgaa tccgttcggc gaggaaccaa ccagctcaat
      661 ttttttgtaa atatgctcag acatgcgccc attatttgct gagcaagtga tcaaaacaac
      721 aaaaagacga gcttcaacgt ggcacagaca tcttgtctgt ggggcaagcg ggcgtctcgc
      781 ctgctgggga tcacgaaatt cgctttacaa aagcatctct ccttgggcag tctcacggag
      841 tgggaaaatc gtttgctgcc tgtctggtgt ttttggttgc tctctcttgc gcccgtgctc
      901 aggaccagga aagcaaactc atcgataggc tgcttagacc ggacacgacg ctgcggaata
      961 atgcgcagaa caaacaattt atcgccgatg gcgcatcgat aaataaaaag gcaaccgttg
     1021 gcacatttta cgtccagaaa aagtccaact cgaagagttt tgccggaaca cgcgattttt
     1081 cagcgcgaca attcaattct caatctttcc agggaacgcg cagcgcctct ggaatttcct
     1141 cacagcaagc gatcggaaat tctcgtcccg cttatgcaac tcgcacggcg cgcggccccc
     1201 gcgatgcccc tcaatctgcc aaaaaggttg ccagcggttc atttgcagga aaccggcctt
     1261 tccttgatca aggcaagagt caaaagtcgc ttaaccggca gaatgcgccg ctgacgatcg
     1321 agcaagtgcg cgagttgctg aacaaaaaca agtagcgcac gggccgggtg cgctattctg
     1381 tggttgatga tgcaaccgga tgaagctctc gcgcttttaa aacacggcgc cgcgcagatc
     1441 attagcgagg atgaactgcg tgagaagctc gcgctcggac gtcccctccg cgtaaaacta
     1501 ggggtcgatc caaccacccg cgacattcat cttggtcaca gtattgttct acgaaagctg
     1561 aggcagttcc aagacctggg ccaccaggcg gttttaatca ttggcgattt caccggtatg
     1621 atcggggatc cgagtggtcg ttccgccacg cgcccgcaat taacgcataa ggaaatattg
     1681 gcaaatgccg caacctaccg tgagcaggca ttcaagattc tcgatccagc gcacacggag
     1741 acggtttgca atggcgactg gtttcgagcg atgtcgttcg aagacgttat tcgcctgaac
     1801 agccgcgtga cgttgcagca aatgttgcag cgtgaggact tccgggcccg gattgataac
     1861 cagcaagcca ttcacgcgca cgaaattcag tatccgatca tgcagggctg ggactcggtt
     1921 atggtgaagg ccgatgtcga gctcggcgga acggatcagc tttttaatat tttggtaggg
     1981 cgcgacttcc aaaaagagga aggtctgccg cagcaagtcg tttttctcct cccgatcctt
     2041 gagggcctgg atggctccaa aaaaatgagc aaaactctcg gcaattttgt cgcgatcaac
     2101 gagcccgcga ccgacgtgtt cgggaagcta atgagcatct ccgatgaatt gatggcgcgc
     2161 tattatccat tgttgctgag tcgttctcta gcgagcgaag cgcatccgct cgaggcaaag
     2221 aagcaactcg ccttcgaaat cgtgcagacc taccactcga ccgaggttgc ccgcaaaacg
     2281 ctcgacgaat ggaacacgcg tttcagccga cgcgagttag aacacgccaa cctgccgatc
     2341 ttcccaggcg ctgcgctgca aaatcttgag gccgtcacgc tgatctcgaa gatttatcag
     2401 gaagcgtttg gcctccaaaa atcccgcagc gaaacttcgc gtctgattaa gcaaggaagc
     2461 gtggaagtcg atggagtaaa gattcgcgac cctaaggcaa cgaccaccct gaaggccggc
     2521 caggttcttc gcctcgacaa gacgcatatg gtgcgaattg gttagaatcc cgcgtacagc
     2581 aaaagagcca cgaaagcgtt ggcctcgtgg ctcttcgaat ttttcgcttc cttaagccgc
     2641 agccgtttgg agcaccggca ttacgcgagc gtgttcaaga tagcttgccg cgatgccttc
     2701 gaagtgcgcc cggatgctgc gcagttcagc cgtgacgaaa ccgtccacaa aacgcaagcg
     2761 ccgatccagc caggcaaaca gcttgttctt gatctcgtag tgaacggcta gcgtgacctc
     2821 cgtgcagttt gggcgaatct cagtgaaatc aacgatcccc atcagatcga tgtttccgcc
     2881 cacggattca aacgcatact gatcaggcga gtcgctttcc accgataaca gaaccgtgtg
     2941 cgcctcaaat ccaagagggc cgcgaaaact aaaatcgacc gtcttggacg acgtaacggt
     3001 cgcccttgtc accattagga actgccttcg atacgattgg atgctggcca gacgagccct
     3061 gcattccgcc aacggttgac tcatatgtaa ggttttttgt aagagcatag gaatatgatt
     3121 ttgttgcgtt gaaacttaag caacagcgat gccatcgcgt ttctccgaag gaagccggtg
     3181 aaatgacgcg gaattgtgta tcatttcttg acacttttgt atccggtcaa gacgctaagg
     3241 cttcctggtc gattcaatca taagtcgcca gtcagccggt agttccatca gagttttacc
     3301 taatccaccc aacaatggaa cgtttacgat ctttcccaaa atatcggcat tggtcagggc
     3361 ggctatgtcg tcggaaaccg gaacgtcatt tagcgcgaca ccaacacagc gtagtttgtg
     3421 ctgggcaacg ctccggaggg tcagcgccgt gtggttaaga caaccaagcc ggttctgcgc
     3481 caccaccaag accggcgact gcatctcaac ggccaaatcg ctcacgaagt agtcagatcg
     3541 tatcggcaca agccagccgc cgacgccttc aacaatgacg tgctcaaccc gaccctgaag
     3601 agcgcgaaaa gcagcaagaa tctgcacgat gtcgatcttc acctcttcca tgaggctgcc
     3661 cacaatcggc gcggctggcg tctccaacca gacgggatta acctcgtcga ttgtcaggcc
     3721 gtcactgcca gctgccagca aaagctctgc gtctcgacgg tctccgcaac aaattggctt
     3781 cattccggcg cagcttattc ccgccgcgcg caataaacgc aacaactgga ccgcagtgtg
     3841 cgtcttacct acgccggtgt ccgtcccggt aatgaaaagg ctcacgcgac ctgttttacg
     3901 cgctccgatg cgcgcgtcac gcgacttgcg ctgatcgttt gcatcatcga ccggaaaatt
     3961 ttcgcgccat cggcactgcc cagccgcgcg tctgacgcgc gatcggggtg aggcatcaag
     4021 ccaaatacgt taccctcgcg attgcagatg ccggcgatgt tctcaataga accattcgga
     4081 ttcgcttcgg caacaatccg gccgtcatcg tccgaatagc gcaaaataac ttgctgattg
     4141 gcattgaggt cgcgcaaagt ttctggatcc gcgaagaagc aaccttcgcc atgcgcaacc
     4201 ggcatgcgga gaggcgatcc tttagggcat ccgtgtgtaa agggcgaatc atccacttcc
     4261 acgcgcattg tgaccatgtc acatacgaat cgcagcgaac gattcctcac gagcgcaccc
     4321 ggcaaaagac cggcctcgca cagaacctgg aacccgttgc acgtcccgag cacgagtttg
     4381 ccagcacgcg cgtcacttat caccgctcgc atgatcggcg aaaagcgcgc gatagcgccg
     4441 caacgaagat aatcgccata cgcgaaccca ccgggaagca cgatcgcgtc gaacccatcc
     4501 agcgacgttt ccttgtgcca gacgtactcg gcgcgc
//
LOCUS       DASQUQ010000254         5582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_973633, whole genome shotgun sequence.
ACCESSION   DASQUQ010000254 DASQUQ010000000
VERSION     DASQUQ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973633"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /gene="dnaK"
                     /locus_tag="VE486_07085"
     CDS             complement(<1..1424)
                     /gene="dnaK"
                     /locus_tag="VE486_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZA38891.1"
                     /translation="MAKVLGIDLGTTNSCMAVMEGGDPVVLENSEGARTTPSVVAFTK
                     TGERLVGQAAKRQAVTNPQNTVFSIKRFMGRKFDEVHEEEHRVPYKIVKAANGDAHVQ
                     VEVNGQRKTFSPPEISAMILSKMKADAEAKLGEKITQAVITVPAYFNDSQRNATKDAG
                     KIAGLEVLRIINEPTAASLAYGLDKKKDEEIAVYDLGGGTFDISVLEIGDGVFEVKAT
                     NGDTHLGGDDWDAKIMDWIVNEFKKGTGIDLTKHPDAIQRIKEEAEKAKIALSSSQEY
                     EINLPFITADASGPKHIQKKLTRAKLEQLTDDLVQRTVPPVKACLNDAKISERDVNEL
                     VLVGGMTRMPKVVETARSLIGKQPHKGVNPDEVVAVGAAIQGGVLKGDVKDVLLLDVT
                     PLTLAIETAGGVATPMIPRNTTIPTRKSQIFSTYSDNQPGVEIKVLQGERPLSRDNKN
                     LGTFHLDGIPPAPRGVPQIEVTFD"
     gene            2139..3365
                     /locus_tag="VE486_07090"
     CDS             2139..3365
                     /locus_tag="VE486_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38892.1"
                     /translation="MARSWSFVRPLLAGLVLTMLQLVMAVVLLAPEGPASYRYSTLIQ
                     HDSYWFMNIVDHGYQTIVPPINQKMMEVSNVAFFPAYPAIAALLHRGLNIDSGNALLI
                     VAQLAAWGFWSYFFLLCRRWRISPVLQVCGALLIAAHPAAFFLITGYSESLFLMTLLG
                     FIYWSSAEGRAAKCFAALHGMVMSATRIVGIVCAAFPVVHSICAGGFKGLREPRAWFR
                     QYGPAIGLMIAATLGALGFFLYCQLRWGRWDIYMLTQAAGWGIVPDYMAVFRPSSYRW
                     LIPALNNPTEASQMSMTLGALLFVGIAICEFLPAIRQRTGLPMRVGIYFCAASIYYIS
                     VSGVACVAMESMLRYEFCVHALIVLALLNYLRQFPTPPMFVRAFGLAAVALISAAGLS
                     VQGWYVWNFTRGNWVA"
     gene            3372..4892
                     /locus_tag="VE486_07095"
     CDS             3372..4892
                     /locus_tag="VE486_07095"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZA38893.1"
                     /translation="MKTKVAIIGAGPAGLTAGYLLSKEEVDVIILEADPVYVGGISRT
                     TTYKGFHFDIGGHRFFSKSKAVEDLWTEILPDDMLVRPRSSRIFYGGKFFSYPLKPFE
                     ALLKLGIFKSVLCVLSWLKARVFPVRNPRNFEEWVSNQFGKRLFNTFFKSYTEKVWGM
                     SCKEISADWAAQRIKGLSLGSAIKNALVPQRYNGDRTNVIKTLINSFRYPRRGPGMMW
                     EACAEKMKAMNGKIEMGCRVTRCSYDEASGSWTVEYKDRQGDPRTIEAEHVISSAPMR
                     ELVCGLSPAVSERTKRAAQSLKYRDFLTVMLILKDRQMFDDNWIYIHDPSVKVGRVQN
                     FRSWSPEMVPDPDKVCYGLEYFCFEHDGLWDSIDSNLVELARRELIKIGLAKEGDLID
                     GCVVRQKKAYPVYDDDYARHVAIIRQELDDRYPNLHLVGRNGMHKYNNQDHAMMTAML
                     CVENILADTKLYDLWQVNGDAEYHEGGPAAAEETIGTGLRLVPTRVVATPELAPEG"
     gene            complement(5028..>5582)
                     /locus_tag="VE486_07100"
     CDS             complement(5028..>5582)
                     /locus_tag="VE486_07100"
                     /inference="COORDINATES: protein motif:HMM:NF044806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38894.1"
                     /translation="LKRASEVAILNANYIAKRLDRYFPVLFKGKRGLVAHECILDLRQ
                     WKSAGIEVEDVAKRLMDYGFHAPTISWPVAGTMMVEPTESEPKHELDRFCDAMISIHA
                     EIGAVTNGKADRENNVLKNAPHTARQIASDKWDRPYSREQAAFPAPWTHEHKFWPAVA
                     RIDNVYGDRNLFCSCPPVEEFENR"
BASE COUNT         1151 a         1519 c         1523 g         1389 t
ORIGIN      
        1 atgtcgaagg tgacttcgat ctgcggaacg ccacgcggcg ccggtggaat cccatcgaga
       61 tggaacgtgc cgagattctt gttatcgcgt gatagcggcc gctcgccttg caagactttg
      121 atttccacgc ccggctgatt gtcgctgtag gtcgaaaaga tctgtgactt ccgagtcggg
      181 atggtagtgt tgcgcggaat catcggtgtg gctacgccgc ccgcggtttc aattgcgagc
      241 gttaacggcg tcacatcaag aagaagaacg tctttgacgt cacccttgag cacaccgccc
      301 tggatcgccg cgcccaccgc gacgacttcg tccggattca cgcctttgtg gggttgtttg
      361 ccgatgagac tgcgagctgt ttcaaccact ttcggcatgc gcgtcatgcc accgacgaga
      421 accagctcgt tcacgtcgcg ttcagaaatt ttcgcgtcgt tcaagcatgc tttcacgggc
      481 ggaactgttc gctgaaccag atcatcagtg agctgctcga gcttggcccg ggtcaacttc
      541 ttctgaatgt gcttcggccc gctcgcgtcc gctgtgatga acggcagatt gatctcgtat
      601 tcctgcgagc tcgagagggc gatttttgct ttctcagctt cttccttgat tcgctggatc
      661 gcgtccgggt gcttggtcag gtcgatgcct gtgcccttct taaattcgtt gacgatccaa
      721 tccatgatct tcgcgtccca gtcgtcaccg ccgaggtgcg tatcgccgtt ggtagctttg
      781 acttcaaaaa ccccatcgcc gatttcgaga accgaaatgt cgaacgtgcc gccgcccaaa
      841 tcgtaaactg cgatctcctc gtcttttttc ttatccaacc cgtaggcgag cgacgcggct
      901 gtcggctcgt taatgatgcg cagcacttcc aggccggcaa tcttgcccgc gtccttggtg
      961 gcattgcgct gggagtcgtt gaaatacgcc gggacggtga tgacggcctg cgtgattttt
     1021 tcgccgagct tcgcctccgc gtcggctttc atcttcgaca aaatcatcgc ggaaatttcc
     1081 ggcggcgaaa aagtcttgcg ctgaccgttc acttccacct ggacatgcgc atcgccattc
     1141 gccgccttca cgattttgta cggaacgcga tgctcttctt cgtgcacttc gtcgaacttg
     1201 cggcccatga aacgcttgat tgagaagact gtgttttgcg gattagtcac cgcctggcgt
     1261 ttcgcggcct ggccgaccag ccgctcgcca gttttcgtaa aggcgacgac cgacggcgtc
     1321 gtgcgcgcgc cttcactatt ttccaaaact accgggtcac caccctccat cacggccatg
     1381 caagagttgg tggttcccaa atcgatacct aaaactttag ccatagaata aatcctttcg
     1441 caggggcgag agttcgaccc aaagaatgtt tagcaagggg taggccaaag cgccgcatta
     1501 ctgctaactc tttgattata aaccaactac gaccagcatt tgccgatcag gtccgctcgg
     1561 gccgagacaa ttaggctcga cttaatcttc cgtagcaatg ggtcatgggc catggcggca
     1621 cacttccctt agcgcatccg cttgcgggaa gtgaaggcgt taccagacgt atttcggtca
     1681 gcgagcgcga gcagtcgcag ttattgcacc gaatttattt gctgaaatta tgtcgccgaa
     1741 ttctattcgc atcacttgag cagcgtaaac acctgcgtgt acgaaccatc cgcatttcgc
     1801 gtactagcta attggccacg cgcgcccatg tacttgccgg tcccgcccca gcagtgcggc
     1861 caacgccatc attctccgtg taataatcag attcattgtg actcctttga acgcattcgc
     1921 gaaaacttgc aattccgaaa ctggaacaca atcagacggg cgttatgacg tttccgcgaa
     1981 taaagcagag agcaatgtcg tcaaaactgc cacccaaaaa gaaacctaca ccgaacccgg
     2041 aaacgatgtc atccgagatt gccttaatcc agagcccggc aagttcctgc agtctcgaga
     2101 gcttgcctat ctcctcggcg cttcctagtc atcagcttat ggctcgctct tggtcgttcg
     2161 tcaggccttt gctcgcggga ttggtcctga ccatgctgca actggtgatg gcggtggttc
     2221 tgctggctcc ggaagggccg gcttcctatc gctattcaac gctgatccag cacgatagct
     2281 attggtttat gaacatcgtc gatcacggct accagacgat tgttccgccc ataaatcaaa
     2341 aaatgatgga ggtttccaat gttgcatttt ttcccgctta tcctgcgatt gccgcgttgc
     2401 ttcaccgcgg actcaacatt gatagtggca acgcgctgct aatcgtcgcc caactcgccg
     2461 cttggggttt ttggagttat ttctttctcc tttgccggcg ttggcgcatt tcgcctgtgc
     2521 tccaagtctg cggcgcattg ttgattgccg cgcatccggc tgccttcttt ttgattaccg
     2581 gctactcaga gtcgcttttt ctgatgacgt tgctcggctt catctattgg agcagcgcgg
     2641 aagggcgcgc cgcgaaatgt tttgctgcgt tgcatggtat ggtgatgtcc gccacgcgga
     2701 ttgtcggaat tgtctgtgcg gcttttccgg tcgtgcactc gatctgcgca ggaggattta
     2761 agggcttgcg tgaaccgcgg gcgtggtttc gccaatacgg accggcgatc ggcttgatga
     2821 ttgcggcaac gctgggtgcg ctcggattct ttctgtattg ccaactgcgc tggggtcgct
     2881 gggacattta catgcttaca caggcggccg gctggggaat tgttcccgat tacatggcgg
     2941 tgtttagacc gtccagctat cgatggctta ttccagcact gaataatccg acggaggcga
     3001 gccaaatgtc gatgactttg ggtgccttgc tctttgtcgg tatcgcaatc tgcgaatttc
     3061 tcccagctat tcgacagcgc accggactac ccatgcgcgt cggcatctat ttctgtgccg
     3121 cgtcgatcta ttacatctca gtcagcgggg tggcctgcgt ggccatggaa agcatgctcc
     3181 gctacgaatt ttgcgtgcat gcgctgatcg tgctggcgct tttgaattat ctgcgtcaat
     3241 ttcctacgcc gccgatgttc gtgcgggcgt tcggtctcgc ggctgtagcg ctgatcagcg
     3301 ccgccgggtt aagcgtacag ggatggtacg tatggaattt cacccggggc aattgggttg
     3361 cgtagaagaa aatgaagaca aaagtcgcca ttatcggtgc gggaccagcg ggtttgacgg
     3421 ccggttacct tctctcgaag gaggaagttg acgtcattat cctggaagcc gatccggttt
     3481 acgtcggcgg gatttcgcgc acgaccactt acaaaggttt tcacttcgac attggcggac
     3541 accgtttttt ttccaaatcg aaagcggtcg aggatttatg gaccgagatt ttgccggacg
     3601 acatgctcgt gcggccgcgt tcgtcgcgca ttttttacgg cggcaaattt ttctcttatc
     3661 cgctgaagcc gttcgaagca cttctgaaac tcggcatctt caaatcggtt ctgtgcgttc
     3721 tttcctggct aaaggcgcgg gtatttccgg tgcggaatcc gcgcaatttt gaagagtggg
     3781 tgagcaatca attcggcaaa cggctcttca acacattctt caaaagctat accgaaaaag
     3841 tttggggaat gagctgcaaa gaaatctccg ccgactgggc ggcgcaacgc atcaaaggac
     3901 tttcgctcgg cagcgcgatc aagaacgcgc ttgtcccgca gcgctacaat ggggatcgaa
     3961 cgaatgtcat caagacgctt atcaattcat ttcgctatcc gcgcagaggt ccaggcatga
     4021 tgtgggaagc gtgcgcggaa aagatgaagg cgatgaatgg gaagatcgag atgggttgta
     4081 gagtgactcg ctgttcttac gacgaagcgt ccggcagttg gacagtcgaa tataaagatc
     4141 ggcagggtga tccgcggaca atcgaagccg aacacgttat ctcatccgcc ccgatgcggg
     4201 agctagtctg cggattatcg ccggcggtga gcgaacgaac gaaacgcgcg gcccaaagtc
     4261 tcaagtatcg cgattttctc accgtcatgc tgatcttgaa agaccggcag atgtttgatg
     4321 acaattggat ctacattcac gatccaagcg tcaaagtcgg tcgtgtccaa aacttccgat
     4381 catggtcgcc cgagatggtg cctgatcccg acaaagtctg ttacggcttg gaatatttct
     4441 gtttcgagca cgacgggctc tgggattcga tcgatagcaa tttagttgag ctagccaggc
     4501 gggagctgat caagattggt ctggccaaag aaggcgacct gattgacggc tgcgttgttc
     4561 gccagaagaa agcgtatccg gtttacgacg acgattatgc gcggcacgtg gccatcatcc
     4621 gtcaggagtt ggacgatcgt tatccgaacc tgcacctcgt cggacgcaac ggcatgcaca
     4681 aatacaacaa tcaggaccac gccatgatga ccgcaatgtt gtgcgtcgaa aatattttgg
     4741 ccgacacgaa gctttacgat ctttggcagg ttaacggaga cgccgaatat catgagggcg
     4801 gccctgcggc ggccgaggaa actatcggca ctggactgcg ccttgtccca acacgcgtcg
     4861 tcgcgactcc cgaactcgcg ccggaagggt aacgcgcgcg tctcgcgtgc tggcaaacgc
     4921 gtcctcgcgg tcgcgaactt ttcttggtgc atttcttgca gaccaaaccg tgcgcaacaa
     4981 aagactgttt cggcgcgacg ccgaaaccaa cacacgagac gtgtgcgcta ccgattttcg
     5041 aattcttcca ccggcggaca ggagcagaat aaatttctgt cgccatacac gttgtcgatc
     5101 ctagcgacgg ctggccagaa tttgtgttcg tgcgtccaag gcgcgggaaa agctgcctgt
     5161 tcgcgagagt agggccgatc ccacttgtcc gaagcaattt gccgcgcggt atgcggtgcg
     5221 ttttttaaaa cattgttttc ccgatccgcc tttccgttgg tgaccgcccc aatctctgcg
     5281 tggatcgaga tcattgcatc gcaaaagcgg tcgagctcgt gttttggttc gctctcggtt
     5341 ggttcgacca tcatcgtgcc tgcgacgggc caggaaatcg tcggtgcgtg aaatccgtaa
     5401 tccattaacc gtttcgcgac gtcttctact tcgattccag cactcttcca ttgtcgcaga
     5461 tcgagaatgc attcgtgggc cacgaggccg cgtttgcctt tgaaaagtac cggaaaatag
     5521 cggtcgagcc gcttcgcgat ataattggca tttagaattg cgacttcgct cgcccgtttc
     5581 aa
//
LOCUS       DASQUQ010000255         4423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1237625, whole genome shotgun sequence.
ACCESSION   DASQUQ010000255 DASQUQ010000000
VERSION     DASQUQ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237625"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VE486_07105"
     CDS             complement(<1..369)
                     /locus_tag="VE486_07105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZA38895.1"
                     /translation="MANTQDIRRRIKSIRNTAQITKAMQMVAASKMRKAQQQALSGRP
                     YAELMNKVLVSLQRRTDPRLHPLLEIRPLKRELVLIISTDKGLAGALNTNLFREAANF
                     DSAKAAFVVTGKKARQFIART"
     gene            complement(477..2009)
                     /gene="atpA"
                     /locus_tag="VE486_07110"
     CDS             complement(477..2009)
                     /gene="atpA"
                     /locus_tag="VE486_07110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZA38896.1"
                     /translation="MSSILEEIETKIEGLKTATTKSNVGVVRETGDGVARIEGLSDVM
                     LNEMIEFPNGIFGLALNLEETEVGAILLGETTKVSEGDEAKTTGKLLQVPVGKVLLGR
                     VVNTLGQPLDGKGPIKTDVFYPLEKIAPGVIKRRSVSQPVQTGIMAIDAMIPIGRGQR
                     ELIIGDRATGKTTIAIDTIISQARLNKAGEEGQIKDYRPLYCIYVAVGQKNSNVARAL
                     AVLEKEGAMPYTTIISAPASDTATNQYLAPFAGAAMGEWFMDNGMDALIVYDDLSKHA
                     VAYRQVSLVLRRPSGREAYPGDVFYLHSRLLERSARLTDEFGGGSVTALPIIETQAGD
                     VSAYIPTNVISITDGQIYLETDLFYQGIRPAISVGLSVSRVGSAAQIKAIKQVAGKVK
                     LDLAQFRELAAFAQFGSDLDTATKARLDRGQRIVELFKQKQYNPIPVEEQVAVMWAMQ
                     NGYVDPVPVERVKEFQLKLQDYLQTRKESLLASIREKKQLDEELESQLKSALDEFKAT
                     WR"
     gene            complement(2022..2414)
                     /locus_tag="VE486_07115"
     CDS             complement(2022..2414)
                     /locus_tag="VE486_07115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZA38897.1"
                     /translation="MKINKEIRQMSREMLRASFTDGQLDPGRIASLVDSLIARKPRNY
                     IDVLKNYRRLLRLEVEKRRARIETASEVDPEATSKVIENLRKKYGSDLTTEFVVNPQL
                     LGGMRIRVGSDVWDGTVRNRLEQLQQQL"
     gene            complement(2415..2948)
                     /gene="atpF"
                     /locus_tag="VE486_07120"
     CDS             complement(2415..2948)
                     /gene="atpF"
                     /locus_tag="VE486_07120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZA38898.1"
                     /translation="MILAANTSFADTVRQTADAFGWTPQMFFSQVISFAIVAFLLRRF
                     AYKPILAVLEERRRKIEEGQLNAEKIKKQLAEAEERYAEILAKANAEAQKMIDEARQS
                     SAHLAERKQQEAIAAAEQIIAKARDASAIEHERTMESLKRELGRLVVDTTAKVTGKVL
                     TAEDQQRLQEETARQLA"
     gene            complement(3004..3219)
                     /locus_tag="VE486_07125"
     CDS             complement(3004..3219)
                     /locus_tag="VE486_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38899.1"
                     /translation="MILPLLAEMTGSIHVGLAAMGVGIGIGLTGLGMTTAVGRNPGAF
                     TPVIVHGILAIALTEAIVFYALYLLAH"
     gene            complement(3272..4270)
                     /gene="atpB"
                     /locus_tag="VE486_07130"
     CDS             complement(3272..4270)
                     /gene="atpB"
                     /locus_tag="VE486_07130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZA38900.1"
                     /translation="MSALAYVAVAPTEKNWGRGISTNRMQYCFSSRLSLALFAVCCSC
                     IFATSATGTEPPPLPETAEHVAVPLKPAPLVKIGKFAVTNSMLVTWIVAAGIIVFAQI
                     ATRRIQPIPSGIQNFWEWLVESLYNFLENIIGRDLVGKTFWFFATLFIFILFVNWFGL
                     IPGVGTIGWGHHDPITGVFHVDRPLLRGGNADLNMTTAMAAIFFVLWFIWAIQAQGVG
                     GFLKHLFAPKGETSGILKILMVFIFFAVGWLEVISILFRPIALSFRLFGNIYAGESIL
                     EALSSMIPALGWLIPIPFYFLEVLVGFVQALVFMLLTAVFTLLIAQHGPGPEREHA"
BASE COUNT          960 a         1252 c         1150 g         1061 t
ORIGIN      
        1 cgtgcgcgcg ataaactgcc gcgccttctt tccggttacc acgaatgctg cctttgccga
       61 atcaaaattc gcggcttcgc gaaagagatt cgtgttcaag gcgccagcca atcccttgtc
      121 ggtgctgatg atcaagacca gctccctctt tagaggcctg atttcaagaa gcggatgcag
      181 ccggggatcg gtgcgtcttt gcagcgagac caggacctta ttcatcaact ctgcatacgg
      241 ccgtccggac agcgcctgct gctgcgcctt gcgcatcttg gacgcagcca ccatctgcat
      301 cgccttcgta atctgcgcag tgttgcgaat cgatttgatc cggcgccgta tgtcctgagt
      361 gtttgccatc tatcgaattt ttgatttgcg atttgcgatt tgcgatttga tcgcgctttc
      421 gacattgtcg attttgcgaa ttgcagacac aactggatca aattcgcgca acgagatcat
      481 cgccaggttg ctttgaattc atcgagcgcg cttttcaatt gcgactccag ctcctcgtcg
      541 agctgtttct tctcgcgaat tgaagccagt aaactttctt tgcgggtctg caggtaatcc
      601 tgaagcttca attgaaactc cttcacccgc tcgactggga caggatcgac atatccattt
      661 tgcatcgccc acatcacagc aacctgctct tcaaccggga tcgggttata ttgtttttgc
      721 ttaaaaagct cgacaatgcg ttgcccccga tcgagtcgcg ccttggttgc ggtatccaga
      781 tcggaaccaa attgcgcaaa cgcggcgagc tcgcgaaatt gcgcgaggtc aagcttcact
      841 ttgccagcga cctgcttgat cgctttgatc tgggcagcgg aaccgactcg gctcacagaa
      901 agcccaactg aaatggccgg ccgaatgcct tgataaaaaa ggtcggtctc gagataaatc
      961 tgtccgtcgg tgatagagat gacgttggtc ggaatatacg ctgagacgtc acccgcttgc
     1021 gtttcgatga tcggaagcgc ggtcaccgag ccgccgccga attcgtcggt caagcgtgcg
     1081 ctgcgttcga gcaaacgcga atgcaaataa aacacgtcgc cgggataagc ttcacggccg
     1141 gaaggccgtc tcagcacaag cgagacctgg cgatacgcga cggcgtgctt ggacaaatca
     1201 tcgtaaacaa tgagtgcgtc cattccgtta tccatgaacc actcacccat ggccgcgccg
     1261 gcaaatggcg cgagatattg attggtggcc gtgtcggacg ccggcgcgga gataattgtt
     1321 gtgtacggca tcgccccctc tttttccaaa acagcgaggg cacgcgccac gttggaattt
     1381 ttctgaccga ccgcgacata aatgcaataa agcgggcgat aatctttaat ctgcccttcc
     1441 tcgccagctt tgttgagccg cgcctggctg atgatggtat cgatcgcgat cgtggttttg
     1501 ccagtcgcac ggtcgccaat aatcaactcg cgctgtccgc ggccaatcgg aatcatcgcg
     1561 tcgatggcca tgatccccgt ttgcactggc tggctcacgc tgcgccgttt gatgacgccc
     1621 ggcgcgattt tttccaatgg ataaaatacg tcggtcttga tcggcccttt gccatccaac
     1681 ggctggccca acgtatttac gacacgcccg agcagcactt ttcccacggg aacttgtaag
     1741 agttttcctg tggtttttgc ttcgtcgcct tctgagacct ttgtggtttc acccagtaga
     1801 atggcgccga cttcagtttc ttcgagattc agggcgagcc caaagatgcc gttcggaaac
     1861 tcgatcatct cgttaagcat cacgtcgcta agaccttcga ttcgcgccac tccgtcgccg
     1921 gtttcgcgca cgacgcctac gttgctcttg gtggtggccg tttttagccc ttcaattttg
     1981 gtttcgatct cttcgagtat gctgctcatg attttgattg cttaaagttg ttgctggagt
     2041 tgttcgagcc tattgcgcac cgttccatcc cagacgtcgc tgccgacccg gatgcgcatc
     2101 ccgccaagaa gctgcggatt gacgacgaat tcagtcgtca gatcgctgcc atatttcttc
     2161 cttagattct cgatgacctt tgacgtggct tcggggtcga cttcgctcgc ggtctcgatt
     2221 cgggcacggc gtttctccac ttcgagtcga agcagacgcc tgtaattttt cagaacgtcg
     2281 atgtaattac gcggctttcg cgcaatgagc gagtcgacga gtgatgcgat ccggccgggg
     2341 tcaagctggc catcagtgaa gcttgctcga agcatctcac gggacatctg ccggatttcc
     2401 ttattgatct tcatctacgc gagttgacgg gcggtttctt cctgcaagcg ctgttggtcc
     2461 tcagcggtaa gcacctttcc ggtgaccttt gcggttgtat ccacgaccag tcgccccagc
     2521 tcccgcttta gggactccat cgtccgttcg tgctcgattg cgctggcatc gcgcgccttg
     2581 gcgatgattt gctccgcagc cgcaatcgct tcctgctgtt tgcgctcggc cagatgggcg
     2641 ctgctttgcc gagcctcgtc gatcatcttt tgtgcttcgg cgtttgcctt ggccagaatc
     2701 tccgcatagc gctcttcggc ttcagccaac tgctttttaa ttttctctgc attgagctgg
     2761 ccctcttcga tcttgcggcg gcgctcctcc agcaccgcaa ggatcggctt gtaggcgaag
     2821 cgtcgcagca ggaaagcgac gatcgcaaag ctgatcactt gcgaaaaaaa catctgcgga
     2881 gtccaaccga aggcgtcggc agtttgccgg accgtgtcgg caaagctcgt gttcgctgcc
     2941 agtatcatca cactcataac ttagacatca ggcgcgcaga gatcttcttc gcgcttcgca
     3001 gcgttaatgg gcaagtaggt agagcgcgta aaacacgatc gcttcggtta atgcgatcgc
     3061 gagaattccg tggacaatga ccggggtaaa ggcccctggg ttgcgaccaa ctgcggtcgt
     3121 cattcccaaa cccgtaagac cgatcccgat acctacgccc atcgcggcga ggccgacgtg
     3181 aatgcttcct gtcatttctg caagtagtgg tagtatcata gttttttttc ttagttagtt
     3241 tggcgagctc gtcgtgagat cgcctaaaaa tttacgcgtg ctcgcgctca gggccagggc
     3301 catgttgcgc gatcaacagt gtgaacactg ccgtcagcaa cataaaaacc agcgcttgca
     3361 caaatcccac caaaacttcc aaaaagtaaa aggggatcgg gattaaccac ccaagtgctg
     3421 gtatcatgct cgacaacgcc tccagaatgc tttccccggc atagatgttt ccgaacaatc
     3481 ggaagctgag ggcgattgga cggaacaaaa tcgagatcac ttcgagccaa cccaccgcga
     3541 agaaaataaa aaccatcaat atcttcagga tgcccgacgt ctcgcctttc ggagcgaaca
     3601 gatgctttaa aaaacctccg acaccttgtg cttggatcgc ccagataaac cagagcacaa
     3661 agaaaatcgc cgccatcgcc gtggtcatat taagatcggc gttgccgccg cggagtaacg
     3721 gcctatccac gtgaaacacg ccagttatcg ggtcgtgatg gccccagccg atcgtgccca
     3781 ctcctggaat cagtccgaac caattcacga acaggatgaa aataaagagc gtcgcgaaaa
     3841 accaaaacgt cttgccgacc aggtcgcgcc caatgatgtt ttctagaaaa ttgtaaaggc
     3901 tttcgaccag ccattcccaa aaattttgga taccgctcgg gatcggctgg atccggcgcg
     3961 tcgcaatctg tgcgaaaaca atgatgcctg cggccacaat ccatgtcacc agcatcgagt
     4021 tcgtcaccgc gaacttgccg atttttacga gcggcgccgg ctttagcggc actgctacgt
     4081 gttcagccgt ttccggtaga ggcggtggct cggtcccggt tgccgatgtc gcaaaaatgc
     4141 aactgcaaca aactgcgaaa agcgccaacg acaaacgtga tgaaaaacaa tactgcattc
     4201 ggttcgtaga aatgccgcgg ccccaattct tttcagtcgg tgcgacggcg acgtaggcca
     4261 gagcactcaa cactcgccgc ggcgagcgtc gcgcatatca aagcgcgttt tgtgggaatg
     4321 acaaacaaaa attctcaatc cgacccctat ttagccggcg cttgctcggc aaactccggg
     4381 gcaaattgag tgagcggctc ggatttggat ggctcggcgg cgc
//
LOCUS       DASQUQ010000256         5441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_163175, whole genome shotgun sequence.
ACCESSION   DASQUQ010000256 DASQUQ010000000
VERSION     DASQUQ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163175"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VE486_07135"
     CDS             <1..808
                     /locus_tag="VE486_07135"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZA38901.1"
                     /translation="DALDLSRRLNDHIAGVVRSHPKRFAGLGTIPLQDPDLAASELER
                     CVRDLHLRGVQIGTHVDANPHTGRIDTLNLDNASLQPVWTVAEQLGAAVFVHPWDMLG
                     KERMPNYWLPWLVGMPAETSLAICSMIFGGVFERFPKLRVAFAHGGGAFPFTIGRIEH
                     AFHVRPDLIAIDNTKNPRSYVAHGKTPARFYVDSLVHDLDALRMLLKLFGAQRIAFGS
                     DYPFPLGEAHPGELIKSIKELSAKERTQLLSGTAREFLGLKAISKEPRNA"
     gene            complement(861..1646)
                     /locus_tag="VE486_07140"
     CDS             complement(861..1646)
                     /locus_tag="VE486_07140"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HZA38902.1"
                     /translation="MPPLTYATYLDLENLLTLQKPRSSPAEHDETLFIIIHQTYELWF
                     KQLLHEFEKIKRDFSAGDLFGAIHSFKRVRTIMKVLVAQVDILETMTPSSFSSFRDRL
                     ETASGFQSVQFREIEFVLGYKRASTLAYVRPDFPGYDRLQQLLRERTVIDHFYDFLAT
                     RGAQIPEELKKRDLTQPNGPNEQVQEEILRLYKSAPECSILFELMTDFDEGLQEWRYR
                     HIKLVERTIGAKKGTGGSPGVPFLKESLFKPVFHDLWAIRHEM"
     gene            1894..4296
                     /locus_tag="VE486_07145"
     CDS             1894..4296
                     /locus_tag="VE486_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZA38903.1"
                     /translation="MAAEVKKEIQLEIAHVLFTDIVGYSKLPITQQRALVERLNEIVR
                     DTDEFQAAETAGRLIKIPTGDGITLVFYHSPEAPVECALEISRALKKYPELQLRMGIH
                     SGPVSGVIDATGKANVAGAGINIAQRVMDCGDAGHILLSKHVAEDLEEYPHWQPHLHD
                     LGECEVKHGVRVSVVNLYTEELGNPAVPEKLNAARLAAAAQRKRAAYRWLSLGMVSLL
                     AVIAVIGFLLFRYSRPPGTAELPVPEKSIAVLPFENRSRDPDNAFFTDGVQDQILTNL
                     AKIADLKVISRTSVMQYKTGAPRNLREIGQQLGVAHLLEGSVQRAANKVRVNAQLIDA
                     RSDAHQWAENYDRDLADVFAIQSEIAKAIADQLQAKLSPNEKKAIEQPPTTDLAAFDL
                     YSRAKTILLTTGFSATNPQEVRQAIELLDQVVKRDPSFFDAYCQLAYAHEYIYAVIGD
                     DHTPARLALAEAALQAATRLRPDAGETHLARAQYLYVGLRDYAGALAELESAWRALPN
                     DPRIFEVTGYILRRRGQQEEGLRYLERAIELDPRNFYTLQQIAISYENLGRFAEAIAA
                     YDRALTIVPDNIETRALRAQDELFWKADTRPLHQTIDSILAQGPGAIAPAADAWFLCA
                     LAARDPAAAERALVALGDNPCWNESAINLSHNFGEGLLARMMKDEARARAAFAAARAE
                     QEKIVQAQPDYGPSLCVLGLIDAALGRKDAALEEGRRAIALVPVEKDVINGSRMIQYF
                     AITAAWAGEKDLALQQLELGLRAPAASVMLSYGALKLHPFWDPLRGDPRFEKIVASLA
                     PR"
     gene            complement(4430..>5441)
                     /locus_tag="VE486_07150"
     CDS             complement(4430..>5441)
                     /locus_tag="VE486_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZA38904.1"
                     /translation="NVCRHRGTRLCEEKNGHLSAIQCPYHAWTYALDGRLIGAPHMDE
                     VPGFDKADFPLHPVNLGLWEGFIFVNLADGGTRLRSEATARQAPGDFISLERWFAPLA
                     GKFTHWNMPILRSAKRVEYDVRANWKLMFENYSECYHCPGVHPMLQKVSPYDSAENDL
                     TEGPFLGGFMKINQGKSLSMSGNACALEIADSARARDRAPTRDRRENKKDEHDYDYEH
                     EHDGKNRVWYYSIFPNMLLSMHPEYVMVHQLWPQSPERTLILCDWFFHPDAFTVSDRG
                     GIPFNPDDAIDFWDMTNKQDWHVCELSQQGIASRAYVPGPYSSRESIPAAWDREYLRF
                     MK"
BASE COUNT         1196 a         1601 c         1511 g         1133 t
ORIGIN      
        1 cgacgcgctc gatctttctc gacgactaaa cgaccacatt gccggagtcg tccgtagtca
       61 tcccaaaaga ttcgccggtc tgggaacgat cccgttgcaa gatcctgatc tggccgcgag
      121 cgagctcgaa cgatgcgtcc gcgatttaca tttgcgcggg gtgcagatcg gcacccacgt
      181 tgacgcgaac cctcacaccg gtcgcatcga cactttgaat ttggacaatg cgtcgcttca
      241 gccggtctgg accgtggccg agcaacttgg tgcggcggtc ttcgtccatc cctgggacat
      301 gctcggcaaa gagcgcatgc cgaattattg gctgccgtgg cttgtcggca tgccggcgga
      361 aacgtcgctc gcgatttgct cgatgatttt tggcggggtc ttcgagcgat ttccgaagct
      421 tcgcgtcgct ttcgcccatg gcggcggcgc gtttccattc acgattggcc gcatcgaaca
      481 tgcatttcac gtgcgcccag atttgattgc gatcgacaac acgaaaaacc caagaagcta
      541 cgtcgcccac ggcaaaacac ctgcgcggtt ctatgtcgat tcgcttgttc acgatcttga
      601 tgcgctgcga atgctgctca agttattcgg cgcccaacgc attgcgttcg gatcggatta
      661 tccgtttccg ctcggcgaag cgcaccctgg cgaacttatc aaatcgatca aggagctctc
      721 cgccaaagag aggacgcaat tgctctccgg cactgcccgc gaattcctcg gactgaaagc
      781 aatctctaag gaacccagga acgcatgaat taaattcttg ttcccgattt cctgctttcc
      841 tcagcgatcg ccacatccag ttacatttcg tgtcgaattg cccacagatc atgaaagact
      901 ggcttgaata gcgattcttt cagaaacggc acacctggtg atccgcccgt gcctttcttc
      961 gcgccaatcg ttcgttcgac caacttgatg tgccgataac gccactcctg tagtccctca
     1021 tcgaaatcag tcattagttc gaacaggatc gaacattcag gtgcgctctt gtagagccga
     1081 agaatttctt cctgcacctg ttcgttaggc ccattcggct gcgtgaggtc tcgtttcttt
     1141 aattcctcag gaatttgcgc accccgcgtc gcgagaaaat cgtagaaatg atcgatcacc
     1201 gtccgttcgc ggaggagttg ttgcagccga tcatatcccg gaaaatccgg ccttacgtac
     1261 gcgagagtgg aggcgcgttt atatccgagg acgaactcaa tttctcggaa ttgcacggat
     1321 tgaaaacccg atgccgtttc gagtcggtcg cgaaagctcg agaacgacga cggcgtcatc
     1381 gtctcaagga tgtccacctg cgccacaagc actttcatga ttgtgcggac gcgcttgaac
     1441 gaatgaatcg cgccgaagag atcgcccgcg gaaaaatctc gctttatttt ctcgaactcg
     1501 tgcagcagct gcttaaacca cagctcgtag gtctggtgga tgatgatgaa cagcgtctcg
     1561 tcgtgctcgg ccggactcga gcggggcttc tgaagagtga gcagattctc cagatcaagg
     1621 taagtggcat aagtcagcgg cggcatagct cgagatactg gatactagat gctggatgct
     1681 cgatgacaaa atcagattct gtatctggga gcgcggacac tgcggcaacc cggtctcggc
     1741 gcctcgccga gacaaacctt gtttcgccac atcttttgcg gcaaggctac tcgttaagga
     1801 ggtaaaaaag gaaattcagc tggaaatcgc gctcgattct cggcagcctc cttgagagca
     1861 tcctcgcgac gaagcatctc catgtaaatc catatggctg ccgaagttaa aaaggaaatt
     1921 cagctggaga tcgcccatgt gttgttcaca gacatcgtcg gttactcgaa gctgccgatc
     1981 actcagcaac gagcgttagt cgaacgttta aacgagattg tccgtgacac ggatgaattc
     2041 caagccgcgg aaacggcggg gcgcttgatc aaaatcccga caggcgacgg catcacgctg
     2101 gttttttatc atagcccgga agcgccggtc gaatgcgcgt tggaaataag ccgcgcgctc
     2161 aagaaatatc cggaacttca gctacgaatg ggcatacaca gtggaccggt cagtggggtg
     2221 atcgacgcaa ccggaaaagc aaacgtggct ggcgccggca tcaacatcgc acagcgtgtc
     2281 atggactgcg gcgacgccgg acacattctg ctgtcgaagc acgtcgccga agatcttgag
     2341 gaataccctc actggcagcc gcacctgcat gaccttggcg agtgtgaagt aaagcatggc
     2401 gtccgcgtct cggtggtgaa tctctacacc gaggagcttg ggaatccggc agtgccggaa
     2461 aagctgaacg cggcgcgtct cgccgctgct gcacaaagaa aacgggccgc gtaccgatgg
     2521 ctgtcattag gcatggtgag tctgctcgct gtgatcgccg tgatcggttt tctccttttt
     2581 cgttatagcc ggccacctgg cacggcagag ttaccagttc ctgagaagag catcgcggtg
     2641 ctgccgtttg aaaaccgaag ccgcgatccg gacaacgcct tcttcaccga tggcgtgcag
     2701 gaccaaatcc tgaccaactt ggccaagatc gcggacctga aggtgatcag tcgcacctcc
     2761 gtgatgcagt acaagaccgg cgcgccgcgc aatctgcgcg agatcggcca acagcttggt
     2821 gtagcgcatt tgctcgaggg cagtgtgcag cgcgcggcca acaaggtgcg ggtgaacgcg
     2881 cagttgattg atgcccgcag cgatgcgcat caatgggcgg aaaattacga tcgcgacctg
     2941 gccgatgtct ttgccattca aagcgaaatc gccaaggcga tcgcggatca actccaggcc
     3001 aaactttcgc ctaacgagaa gaaggcgatt gagcagccgc ccaccaccga cctggcggct
     3061 ttcgaccttt acagccgggc caagactatt ctcctcacta caggcttcag tgcgaccaac
     3121 ccgcaagagg tgcggcaggc aatcgagctc cttgatcagg tggtaaagcg cgacccgtca
     3181 tttttcgacg cctactgcca gctcgcctat gcgcacgaat acatttatgc agtgatcggc
     3241 gacgatcata ctccggcccg actcgccctc gccgaagctg ctctccaggc cgcgacacgg
     3301 ctgcggccag atgccggcga gactcatctg gcgcgcgcgc aatatctcta cgtcgggctg
     3361 cgcgactacg ccggcgccct ggccgaactg gagagcgcat ggcgagcttt accgaatgat
     3421 ccgcgcatct tcgaagtgac cggctacatc ttgcgccggc gcggccagca ggaggaaggg
     3481 ctgcgctatc tggagcgcgc gatcgagctt gatccgcgca acttttatac tctgcaacag
     3541 atcgcgatca gttatgaaaa cctgggccgc tttgccgagg cgatcgccgc ttacgatcgc
     3601 gcgctaacca ttgttcctga caatatcgaa acgagggctc ttcgtgcaca agatgagctc
     3661 ttctggaaag ccgacactcg tccgctccat cagacgattg attcaatcct ggcgcaaggt
     3721 cctggcgcga ttgcgcccgc ggccgatgcc tggtttctgt gcgcgctggc cgcgcgcgat
     3781 ccggctgcgg ccgagcgggc cctggtggcg ctgggcgaca atccctgctg gaacgaaagc
     3841 gccatcaatc tgagccacaa cttcggcgaa ggtctgctgg cgcggatgat gaaagacgaa
     3901 gcaagagcgc gcgctgcttt tgccgcggcg cgcgcggagc aggaaaaaat cgtgcaagca
     3961 cagccggact atgggccgtc gctctgcgtc ctcggcttga tcgacgccgc gttgggacgc
     4021 aaggacgcag ccctggagga aggacgacgc gcgatcgcac ttgttcccgt ggaaaaggac
     4081 gtcatcaatg gcagtcgcat gatccaatac ttcgcgatca ccgcggcatg ggcaggcgag
     4141 aaagaccttg ctttgcagca actggaactc gggctgcgcg ctcccgccgc ctcggtcatg
     4201 ctgagctacg gcgcattaaa gctccatccc ttctgggacc cgctccgcgg cgatccgcgc
     4261 ttcgaaaaaa tcgtcgcctc gctcgcgccg agataggggc ggttgaccac aatcgcctac
     4321 aaatgccaat ccgggtcgga tcgattcagt gaatggagcg agccgcgaca tagacgggaa
     4381 gcccgagagg gtgagtgagc gggagcgaac gaatcaaccg cccctacctt catttcatga
     4441 agcgcagata ttcccgatcc catgccgcgg ggatgctttc cctcgacgaa tacggccccg
     4501 gcacatacgc gcgcgacgcg atcccttgct ggctcaactc gcacacgtgc cagtcctgct
     4561 tgttcgtcat gtcccaaaag tcgattgcgt cgtccggatt gaaggggata ccgcctcggt
     4621 cgctgaccgt gaacgcgtcc ggatgaaaga accaatcgca taggatcagc gtccgttccg
     4681 gcgactgcgg ccagagctga tgcaccatca cgtattccgg atgcatcgaa aggagcatat
     4741 tgggaaaaat cgagtagtac cacactcgat tctttccgtc gtgctcatgc tcgtaatcgt
     4801 aatcgtgctc gtctttcttg ttttcccttc gatcacgagt aggagcacga tcacgagcac
     4861 gagcggaatc tgcgatttcc agtgcgcagg cgttgccgct catgctcaga ctcttgcctt
     4921 gattgatctt catgaacccg ccgaggaacg gaccttcggt cagatcgttc tccgccgaat
     4981 cgtacggtga aactttctgc aacatcggat gcacgcctgg gcagtgataa cactccgaat
     5041 agttctcgaa catcaacttc caattggccc gcacatcgta ttcaacccgt ttcgccgagc
     5101 gcaagatcgg catgttccaa tgagtgaact tccccgccaa cggcgcgaac catcgctcta
     5161 gggagatgaa atctcccgga gcttgccgcg ccgtagcttc tgagcgaagg cgggtccccc
     5221 cgtccgccaa attcacaaag atgaatcctt cccaaagtcc gaggttcacc ggatgcaatg
     5281 gaaaatcggc cttatcgaaa cccggcacct cgtccatgtg cggcgcgccg atcagccggc
     5341 catcgagcgc gtacgtccac gcgtgatatg gacattgaat cgccgacaaa tgtccgtttt
     5401 tttcctcaca caaacgtgtc ccacgatgtc gacacacgtt a
//
LOCUS       DASQUQ010000257         3778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_434551, whole genome shotgun sequence.
ACCESSION   DASQUQ010000257 DASQUQ010000000
VERSION     DASQUQ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434551"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2487
                     /locus_tag="VE486_07155"
     CDS             <1..2487
                     /locus_tag="VE486_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZA38905.1"
                     /translation="TAAGDTKAFSPPKDTRIIDCKGKFILPGYIDTHVHFFQSGDLYT
                     RPDAVDLTKVRPYADEVAWIKKNLPDTFARYLRSGITSVVDVGGPFWNFEVRKTADAT
                     PKAPRVAVAGPLISSVSRPQLDLGDPPIVKIDDPQQAREFVRKLAAQKPDLVKIWYIV
                     DKDHPLDSFRPIVRATIEESHAHKIRVAVHATELETARAAVQEGADVLVHSVTDKEVD
                     DAFVKLLKEHQTILTPTLVVFERYGRTFANKLNLTPEEKAWGNPEVIATLDVMKLPHD
                     QLPERIKTALADPNAALDRIKKVQDVALKNLKTLEDAGVTIAAGTDAGNIGTLHGPAL
                     FREFQLMKEAGLTPMQILQCATANAAKLFGGETGAHIGQVEKGYFADLVILNSNPLHD
                     IAHASDIDTVIKNGVLYPIRSLENAWNDSPLADINALAENYVKLVLAMGQHDPDYVDA
                     YYGPPELKTDAHVKKSLDAIAVEAARLRDQVAKIPEPKDEMERLRREYLIKQLSALTT
                     RVRIVKGEHLKFDEESLALYDAVAPTLPESHFQEILARLEPKLPGDGALFRRYENWRR
                     AFVIPNEKLDTVFQLAIKACRERTLAHINLPPTENFTVEYVTNKPWGAYNWYQGNYRS
                     VIQVNTDLPAYIDRAIDLAAHEGYPGHHVYNALLEKNLVRDRGWVEFSIYALFSPQSL
                     IAEGTANFGKEVVFTKPERLEFEKKVLWPAAGLDPSRAEEFYAVQELVKELDYAANEA
                     ARRYVNGEIDAKTVATWLQKYALMDEKRAKQRTKFIEKYRSYVINYNLGEDMVRRYIE
                     KRGGTEQQPETRWREFEQLLASPRLPGDIR"
     gene            2572..3441
                     /locus_tag="VE486_07160"
     CDS             2572..3441
                     /locus_tag="VE486_07160"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZA38906.1"
                     /translation="MTTNPTQYAHPEALLETDWLEKHLNDEGIRIVESNEDILLYDTG
                     HIPGAVHIDWRADLQDPVIRDYISPEKFARLCSRHGITPDTTCIFYGDKANWWACYAL
                     WAFRLFGHEKVKILNGGRDKWKAERRPMTREIPKYPETNYPVPKKRFDKEIRAFFDEA
                     LAQSKGGKPLIDVRSPGEYKGEITHMPEYPQEGVLRGGHIPGAKSVPWKTATNDDATF
                     KPADELAKIYLDQCGVKPGTDTVVYCRIGERSSHTWFVLTYLLGLDNVRNYDGSWTEW
                     GNKVGAPIEKNGG"
     gene            3581..>3778
                     /locus_tag="VE486_07165"
     CDS             3581..>3778
                     /locus_tag="VE486_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38907.1"
                     /translation="MYPQKLNNIINLFETLPEDERRETLVSYADNAKRQEPREGENFD
                     LVDVRKDEDCTDTVGVYLHVDE"
BASE COUNT          930 a         1032 c         1088 g          728 t
ORIGIN      
        1 actgcagccg gtgataccaa ggcattcagc cctccgaaag acacccgcat catcgattgc
       61 aaaggcaaat tcattttacc gggctacatc gacacgcacg ttcacttctt ccaatcgggc
      121 gatctctata cacgcccgga cgctgtcgat ctcaccaagg tgcggccgta cgcggatgaa
      181 gtcgcgtgga tcaaaaagaa tttgcccgac acgtttgcac gctatctacg cagtggaatt
      241 acgtcggttg tcgatgtggg cgggccgttt tggaattttg aggtgcgcaa aactgcggac
      301 gcgacgccga aagcgccgcg cgttgcggtc gcgggaccgt tgatctcgag cgtgtcgcgg
      361 ccgcaactcg atctgggcga tccgccgatc gtcaagatcg acgatccgca acaagcgcgc
      421 gagttcgtgc gcaagctcgc cgcgcaaaaa cccgatctcg tgaaaatctg gtatatcgtc
      481 gataaagatc atccgctgga cagctttcgt ccgatcgtcc gcgcgacgat tgaagaaagt
      541 cacgcgcaca aaatccgggt ggcggttcat gcaaccgaat tagaaacggc gcgagccgca
      601 gttcaggaag gcgccgacgt gctcgtgcac agcgtcaccg acaaggaagt cgatgacgca
      661 ttcgtaaaac tgttgaagga gcatcagacg attctgacgc cgacactcgt ggtgtttgaa
      721 cgctacggcc gcacgttcgc aaataagttg aatctgacac cggaagaaaa agcgtggggc
      781 aacccggaag tgatcgcgac cctcgacgtg atgaaactgc cacacgatca attgcctgag
      841 cgcatcaaaa ctgcgcttgc cgatccaaat gccgcgctcg atcgaatcaa aaaagttcag
      901 gatgtcgctc tgaagaatct gaagacgctc gaagacgcgg gcgtcaccat cgctgcggga
      961 acggatgccg gaaacatcgg cacacttcat ggcccggcgc tgttccgtga gtttcaactg
     1021 atgaaggaag ccggtctgac gccgatgcag attctgcaat gcgccaccgc gaacgccgcg
     1081 aagcttttcg gcggcgaaac gggtgcgcat attggccagg tcgaaaaggg ctatttcgcc
     1141 gatctcgtca tactcaattc gaatccgctg cacgacatcg cacacgcttc cgacatcgac
     1201 acggtaatca agaatggcgt cctttatcca attcgctcgc tcgagaatgc gtggaacgat
     1261 tcgccgctgg cagacataaa cgcgcttgcg gaaaattacg tcaagctcgt tctcgcgatg
     1321 gggcagcatg acccggatta cgttgacgct tattatgggc cgccggaatt gaagacagac
     1381 gcgcacgtaa agaaatcgct cgacgcgatc gcggtggagg ccgcgcgatt acgcgaccag
     1441 gtggcgaaga ttcccgagcc gaaagatgag atggagcggt tgcggcgcga gtatttaata
     1501 aagcaattgt ccgcgctgac gacacgagtg cgcatcgtca aaggcgaaca cctaaagttc
     1561 gatgaagaat cgctggccct ttacgacgcg gtcgcgccga cgctgccgga atcgcatttc
     1621 caggaaatcc tcgccaggct cgagccgaaa cttccgggtg acggagcgct ctttcggcgt
     1681 tacgagaatt ggcgtcgcgc gttcgtgatt ccgaacgaga aactggacac ggtatttcag
     1741 ctcgcgatca aggcctgccg cgaacgcacc ctcgcgcaca tcaatttgcc gccgaccgaa
     1801 aatttcacgg tcgaatacgt cacgaacaaa ccgtggggcg cttacaattg gtatcaaggc
     1861 aactatcgga gcgttattca ggtcaacacc gatttgcccg cttacatcga tcgcgccatc
     1921 gatctggccg cgcacgaggg ttaccctggc caccatgtct acaacgcgct gctggagaaa
     1981 aatctggtgc gcgatcgcgg ttgggtggaa ttctcgatct atgcgctctt ctcgccgcaa
     2041 tcgctcatcg ctgaaggcac cgccaacttc ggtaaggagg tcgtatttac aaaaccggag
     2101 cgtctggagt tcgagaaaaa agtgctctgg ccggcggcgg gactcgatcc ctcgcgcgcg
     2161 gaagagtttt atgcagtcca ggagttggtg aaggaactgg attacgccgc gaacgaagcg
     2221 gcccggcgtt atgtgaatgg cgagatcgac gcgaagacag tcgcgacgtg gttgcaaaaa
     2281 tacgcgctca tggatgagaa gcgggcgaag cagcggacaa agttcatcga aaaataccgc
     2341 agctacgtga tcaactacaa tcttggcgaa gacatggtgc gcaggtacat cgagaaacgc
     2401 ggcggtaccg aacagcagcc ggagacacgc tggcgcgagt tcgagcagtt gctggcgtcg
     2461 ccacggttgc cgggcgatat acgctaggat cgacaacggc tgctcgttag ggcgttggga
     2521 aagtaaaacc tcttgcccgc actggaaagc ggcccgaaat ttccagttct tatgactacc
     2581 aatcctacgc aatacgcgca cccggaggcg ctgctggaga ccgattggct ggagaaacat
     2641 ctcaacgatg agggcatccg gatcgtcgag tcgaacgaag atattctgct gtatgacacc
     2701 ggacatattc ccggtgcagt ccacatcgat tggcgcgccg atctgcaaga tccggtgatt
     2761 cgcgattaca tttcaccgga aaagtttgcg aggctttgca gccggcacgg gatcacgccg
     2821 gacacaacct gcatttttta cggtgacaag gcgaactggt gggcctgtta tgcgctctgg
     2881 gcgtttcgct tgtttggtca tgagaaagtg aaaattctca acggcggccg cgacaaatgg
     2941 aaagccgaac ggcggccgat gacccgcgaa attccaaaat atccagagac aaattatccg
     3001 gtgccgaaga aacgcttcga caaagagatt cgcgcgtttt tcgatgaggc gctcgcccaa
     3061 agcaagggcg gaaaaccgtt gatcgatgtg cgctcccctg gcgaatacaa aggcgagatc
     3121 acccacatgc cggaatatcc gcaggaaggc gtgctgcgcg gtggtcatat tcccggcgcg
     3181 aagagcgtgc cgtggaaaac ggccacgaat gacgacgcca catttaaacc cgcagatgaa
     3241 ctggccaaaa tttatctcga tcaatgcggg gtaaagccgg gcacagacac agtcgtttat
     3301 tgccggatcg gcgaacgctc cagtcacacc tggtttgtgc tcacctatct gctcgggctc
     3361 gataacgtgc gcaattacga cggttcatgg accgaatggg gcaacaaagt cggggcgccg
     3421 atcgaaaaga acggaggtta gccatccttg gctgactcgg cagacaagct tccagcttgt
     3481 cgtctgggag acccggcagc ctggaaggcc gccggccgag tcaggctgga agcctaacct
     3541 ccggtgaaat ccgtaaatcc gtggtagatt ctcattcttg atgtacccac aaaagctcaa
     3601 caacatcatc aatctcttcg aaacgctccc agaagatgag cggcgcgaga cactcgtctc
     3661 ctatgctgac aacgcgaaga gacaggagcc gcgcgaaggc gagaatttcg atctcgttga
     3721 tgtccgcaag gatgaagatt gcacggacac ggtcggcgtt tatctgcatg tcgatgag
//
LOCUS       DASQUQ010000258         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1153477, whole genome shotgun sequence.
ACCESSION   DASQUQ010000258 DASQUQ010000000
VERSION     DASQUQ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153477"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VE486_07170"
     CDS             complement(<1..562)
                     /locus_tag="VE486_07170"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZA38908.1"
                     /translation="MHQLLEIWFSWVLTGGYTGIIVLMAMESSIFPVPSEIVIPPAAF
                     LAAQGKLSFAGVVLAGTLGSYLGSAITYWASRLIGRPLIIKYGRFVFLTPKKLERAEH
                     WLARYEAGGIFFARLLPVVRHLISIPAGIVRMNFGVFSLMTVAGSAIWCYILAYLGEK
                     AYRVEPELLTDPEALVRFIHGQSTWIL"
     gene            664..2406
                     /locus_tag="VE486_07175"
     CDS             664..2406
                     /locus_tag="VE486_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZA38909.1"
                     /translation="MSIYRRVLRYYRPFLGQTIFGLLLSLIGICLNLLKPWPFKIIVD
                     DFLRAGPATSGNWRVWIPLLCLALVAIQLLWGIINWVTNYLFVKIGLQALLKLRTDLY
                     SYLQSLSLKYHDARRSSDSSFRVAYDSQSIQTIYNKGFTNVFSSIVTLIGTFVIMLQL
                     DWKLTFVSLAIVPLIVGAIYLFAHRIRRESTSIQEQESAMLAQAQEGLSSIRMVHAFG
                     REEFEVRQFHQQARQSLQANLRLTLTNVNSALVISTLMVIGTAAMYYIGALHVLAGTL
                     TLGSLLVFSAYLLMLYQPLESLTYTAWAMEGATAGAKRCFEVLDRQDDVIDSPGAIAI
                     SSAKGAIVFKSVTFGYAADRTVVRDIGLRIDPNQIVGLVGGTGAGKSTLLGLVPRFYD
                     PTTGSITLDGRDLRQVTKKSLRAQIAIVLQDTLLFSTTVRENIAYGRPDATEEEIVEA
                     ARRAQADDFIQQLPNGYASPVGERGGHLSVGQRQRIGIARAFLKNAPILLLDEPTSAL
                     DPVTELAIMETIKELMRGRTTLIATHRLAAIHDVDQIVVLEDGRIVEKGRGPELVRCG
                     GIYAKLYTSGKFPS"
     gene            2403..2993
                     /locus_tag="VE486_07180"
     CDS             2403..2993
                     /locus_tag="VE486_07180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZA38910.1"
                     /translation="MSNVTPDRHVEDDWWPHPIPGNVEFGEGFYCESAQVFRKLRSKK
                     PRAVIIGNHVSCYAGCSFSIGELGQCTIGDFTLLNGALIMAEDKIDIGSHCLISWNVG
                     IADSDFHPLDPAQRLVDAQALAPYFKDRPPRPKLRTAPVKIADNVWIGMNAVILKGVT
                     IGENCVVAAGAVVTKSVEPNSVVAGNPAVIVKKFQT"
BASE COUNT          701 a          849 c          806 g          713 t
ORIGIN      
        1 ccagtatcca agtcgactga ccatggatga agcggaccaa tgcttccggg tcggtcaaaa
       61 gctccggctc gacccgatac gccttctcgc ccaggtaagc caagatgtaa caccaaatcg
      121 ccgagccggc gacggtcatc aggctgaaga cgccgaagtt catgcgaacg atacccgctg
      181 gaatggagat cagatgccgc acaaccggaa gcagccgcgc aaaaaagatc ccaccggctt
      241 cgtagcgggc aagccaatgc tccgctcgtt cgagcttttt cggagtcaga aatacgaacc
      301 gtccatattt tatgatcagc ggcctgccga ttaatcgtga cgcccagtac gtgatggctg
      361 agcccagata cgatccgagc gttcccgcca gaacgacgcc cgcaaaactg agtttgccct
      421 gagccgccag aaatgcggcc ggtggaatga caatttcgct tggcacagga aagatcgaac
      481 tctccatcgc catcaagaca ataattccag tgtagccgcc ggtcaggacc cagctgaacc
      541 agatttcaag aagttgatgc atattgtcat tcccagcgaa gtcgaggaat ctctcgatat
      601 cgaaccgcaa ggagttcgcc gtagttcaat ccaaaattca aaatccgcaa tctgaaattc
      661 accatgtcga tctatcggcg cgtcctccgt tattaccgcc cgttccttgg gcaaacgatc
      721 ttcggattgc ttctgtcgct tatcgggatt tgcttgaatc tgcttaagcc atggccgttt
      781 aaaataatcg ttgacgattt cctccgggcc ggcccggcaa catctggcaa ttggcgtgtg
      841 tggattcccc tgctgtgtct ggcgctggtc gcgatccagc tcctctgggg aattatcaat
      901 tgggttacga attatctttt tgtgaaaatc gggctacaag cgttgctcaa gctgcgcacc
      961 gatctctatt cctatctgca atctctctca ctcaaatatc acgatgcgcg gcgttcgagc
     1021 gactccagtt tccgagtcgc ctacgattcc caatcgatcc agacgatcta caacaaaggt
     1081 ttcacaaacg tcttctcatc gatcgtcaca ttgatcggaa cattcgtcat catgctccag
     1141 ctggattgga agctgacatt tgtttcgctt gcgattgttc ctctgatcgt cggcgcgatt
     1201 tatctttttg cgcatcgaat tcgtcgcgaa tcgacatcca ttcaggaaca ggagagcgcg
     1261 atgctggcgc aagcgcagga aggcctgagc tcgatccgga tggtgcacgc atttgggcgt
     1321 gaggaatttg aagtgcggca attccatcag caagcccgtc aaagcttgca agctaatctg
     1381 cggctcacgc tgacaaacgt gaacagcgcg ctcgtgatca gcaccctgat ggtgatcggc
     1441 acggcggcga tgtattatat cggcgctttg catgtgctcg ccggcacgct caccctcggt
     1501 tccctgcttg tcttcagcgc gtatctgctg atgctttatc aaccactcga gtcgcttact
     1561 tacacggctt gggcgatgga aggcgccacc gccggcgcga aacgctgctt cgaagttctg
     1621 gatcgacagg atgacgtgat tgattcacct ggcgcgattg cgatttcatc cgccaaaggc
     1681 gccatcgtct tcaagtcggt aaccttcggt tacgcggcgg atcgcactgt ggtgcgagat
     1741 ataggtcttc gcatcgatcc caatcagatc gtcggccttg taggaggcac cggagccggc
     1801 aagagcactt tgcttggttt ggtgccgcgc ttttacgatc ccacgaccgg ctcgattacg
     1861 ctcgatggac gtgatcttcg ccaggttaca aagaaaagtt tgcgcgcaca gatcgcgatc
     1921 gtcctgcaag acacattgct tttttcaaca acggttcggg aaaacatcgc ctatggccgg
     1981 ccagatgcga cagaagaaga aattgtcgag gccgcgcgtc gtgcccaggc cgatgacttc
     2041 attcagcaat tgccaaacgg ctatgcaagt cccgtcggcg aacgaggcgg gcatttgagc
     2101 gtcggccagc ggcagcgcat tggcattgca cgcgcatttt tgaaaaacgc gccgattttg
     2161 cttctagacg agcccacttc agccctcgat ccggttacgg aattggcaat catggaaacg
     2221 atcaaagaat tgatgcgcgg ccggaccact ctgattgcca ctcatcgcct ggcagccatt
     2281 catgacgttg atcaaatcgt cgtcctcgag gacggacgga tcgtcgagaa gggccgtggc
     2341 ccggaactgg tccggtgtgg cggtatttac gcgaaacttt atacttcggg aaaatttcct
     2401 tcatgagcaa tgtcacaccg gatcgacatg ttgaggacga ttggtggccg cacccgattc
     2461 caggcaacgt cgagttcggc gaaggatttt attgtgagag cgcgcaagtc ttccggaaac
     2521 ttcgcagcaa aaaaccccgg gcagtaatta tcggcaacca tgtttcctgt tatgccggct
     2581 gctcgttttc tatcggtgaa ctgggccagt gcacaatcgg cgatttcact ctgctcaacg
     2641 gcgcgctcat catggcggag gacaagattg acatcggctc gcactgcctc atttcctgga
     2701 acgtcggaat cgccgactcc gattttcatc cgctcgatcc cgcgcaacgc ctggtcgacg
     2761 ctcaggcttt ggcgccttat ttcaaagatc gcccgccgcg cccaaaatta agaaccgctc
     2821 ctgtgaaaat cgcggacaat gtttggattg ggatgaacgc cgtcattttg aaaggcgtca
     2881 ccatcggcga aaattgtgtg gttgccgcgg gtgcggtggt gacgaaatcc gtcgagccga
     2941 acagcgtcgt agcgggtaat ccagctgtaa tcgttaagaa attccagacg tgatccaatc
     3001 caacatcgaa catcgaacat cgaacatcga atgccgaatt ttctgcattc gatattgggc
     3061 gttcgatgt
//
LOCUS       DASQUQ010000259         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_539219, whole genome shotgun sequence.
ACCESSION   DASQUQ010000259 DASQUQ010000000
VERSION     DASQUQ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539219"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..662)
                     /locus_tag="VE486_07185"
     CDS             complement(12..662)
                     /locus_tag="VE486_07185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZA38911.1"
                     /translation="MSFDLVAPHYRWLETIAFGNALQRARTCWIDTIARPKRTLIFGE
                     GDGRFLCELVRAFPTIDVDCIDASEAMLQLARARLRRTHPESFSRVHFFREDILKWSP
                     RKSYDLLVTHFFLDCFSGRELRAIIAKLASAAEPGAVWLIADFTIPRRRFARAHARFW
                     LRMMYTFFRATAGIAANELVDPIPYLDGHGFTRASGNLSRGSMLRSDVYVAAIGAC"
     gene            complement(659..1558)
                     /locus_tag="VE486_07190"
     CDS             complement(659..1558)
                     /locus_tag="VE486_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38912.1"
                     /translation="MHFTRQIDPRATMVMRGLQDRTVSRSPWLWLNLVCLDAPLVAIC
                     WQWIFAHSFHLSVPVGHRAALFLTAWVIYLADRFGDSVSLITGQPKSVRQQFCLRHKS
                     IWLVSIICVAAVDLVVVLRAVNYDTAVPGTVLGALTVAYVAINHAHSEIWEIIPLKEF
                     MIGSLFAAGTLLGVTPHIFTESSTMIFAGILFAALCSLNCISIAIWERDLDRIQGKHS
                     IATRWPHANFLARLLLLLLLAGSVVLALFDAGAWPVVLCLGASGLLLGALRFVPVSRD
                     ERTALADLVLFTPLALFVAEKVL"
     gene            1587..1814
                     /locus_tag="VE486_07195"
     CDS             1587..1814
                     /locus_tag="VE486_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38913.1"
                     /translation="MDDRIFSNFFVPELGRSRAIARRYGNVPGIWLVILAAFAVQGCN
                     RSDEEQRARRMASRVELESVIRMNNLNNYPD"
     gene            1946..2422
                     /locus_tag="VE486_07200"
     CDS             1946..2422
                     /locus_tag="VE486_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38914.1"
                     /translation="MSASLPAGLGTKLRQLITRLDGDVQQLYGEMNAPFRPRFYPVVR
                     LLLLKPQTVNSIAAQVGVSQPAITQTLGEMRKIGLIAAKPGSDRRKQIIHLTRKGESV
                     AEKLQPVWNATHRAAAGLDADLAARLGEIVDQALAALDERPFKERIRIQLSTKNHD"
     gene            2415..3371
                     /locus_tag="VE486_07205"
     CDS             2415..3371
                     /locus_tag="VE486_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZA38915.1"
                     /translation="MTRLLISALLAGYATATALSPNDQSEVVRSAARVIEKRYVDPER
                     AHEIARKLRDAESRWSHVTDPKSFAGAVTKWLREISGDGHLGLSYSEKVLPEQTGDES
                     FSTAEMEKYYGAHLNHGIEKIERLPGNIILLDIRVFPPSPMAGDVFAAVMTVVAQGDA
                     LIIDLRKNGGGAETANLVTGYLLDGGKPLSGDYNRPTDRHSANISPSWVPGRRFGSTK
                     PLYILTSRKTFSAAEAIAYDLQALKRATIVGEVTGGGAHPFEYRRVHPHFAVDLPEGR
                     SINPITKTNWQDVGVQPDIPVPAEQALDKALELARAAIRKNR"
     gene            complement(3381..3456)
                     /locus_tag="VE486_07210"
     tRNA            complement(3381..3456)
                     /locus_tag="VE486_07210"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3421..3423),aa:Thr,seq:tgt)
     gene            3697..>4103
                     /locus_tag="VE486_07215"
     CDS             3697..>4103
                     /locus_tag="VE486_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004571 - mannosyl-oligosaccharide
                     1,2-alpha-mannosidase activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HZA38916.1"
                     /translation="MNERSGQKDWKELSAVVKNLESMSVKPIRGSPATKPTRRTLVFA
                     ISLVILAAFAGQGCNRSGEDAQARRMAASVKAEFLHAWRNYERYAWGHDALQPLSKTP
                     HDWYEQSLLMTPVDALDTLVLMNLDADVAKTKEL"
BASE COUNT         1001 a         1136 c         1152 g          814 t
ORIGIN      
        1 tcaatggcgc cttaacaggc gccgatcgca gcgacatata cgtcggatct cagcatactt
       61 ccccgcgaca gatttcctga agctcgggta aacccgtgtc catccaaata cggaatcgga
      121 tcgacaagtt cgttcgccgc aattcccgca gtcgcgcgaa aaaacgtgta catcattcgc
      181 agccagaatc tggcgtgcgc tcgggcaaat ctccgccgcg ggattgtgaa atcggcgatc
      241 aaccaaacag cgccgggctc ggccgcgctc gccagtttgg caatgatcgc tcgtagttct
      301 ctgccggaaa aacaatcgag aaaaaagtgc gtcaccaaaa ggtcgtatga ttttcgcggt
      361 gaccatttca gaatgtcctc acggaaaaaa tggacgcgtg aaaacgattc cggatgcgtc
      421 cggcggaggc gggcacgggc gagctggagc atggcttcgc tcgcatcgat gcagtcgaca
      481 tcgatcgtcg ggaaggcgcg cacaagctcg caaaggaaac ggccgtcgcc ttcaccgaag
      541 atcagggtgc gtttcgggcg cgcgatcgtg tcgatccagc aagtccgggc gcgttgcaac
      601 gcgttaccga aggcgattgt ttcgagccag cgataatgcg gtgcgacaag atcgaagctc
      661 acaagacttt ctccgcaacg aacagcgcga gtggcgtaaa gagaacgaga tctgcaaggg
      721 cggtgcgttc atctcgtgac accgggacaa aacgcagcgc gcctaacaaa aggccgcttg
      781 ctccaagaca gagcacgact ggccaggcgc cagcatcgaa cagggcgagc acgacagaac
      841 ctgccagcaa gaggagtaag agcagccgag cgaggaaatt tgcatgcggc caacgcgtcg
      901 cgattgaatg cttgccttga atccgatcga gatcccgttc ccagatcgca atgctgatgc
      961 agttcagcga acatagcgca gcaaagagga tccccgcgaa aatcattgtc gagctttccg
     1021 taaaaatatg cggggttacg ccgagcaaag tgccggcggc aaacaacgag ccgatcatga
     1081 attccttcag cggaattatc tcccaaattt cgctgtgcgc gtgattgatc gctacatagg
     1141 cgactgtcag tgcacccaga actgtgccgg gtacagccgt atcgtagttc acggctcgca
     1201 aaacaacgac aagatcgacg gcggcgacac agatgatgga gacgagccaa atacttttgt
     1261 gtcgcaagca gaactgttgt cgcaccgatt tcggttgccc tgtgatcagc gaaactgaat
     1321 cgccaaaacg gtccgccaga tagatgaccc atgccgtaag aaacagcgcg gctcggtgcc
     1381 caaccggcac ggacaaatgg aagctgtgcg cgaaaatcca ctgccagcag atcgcaacca
     1441 atggcgcatc gaggcagaca agattcaacc acagccatgg actgcgcgag acagtgcgat
     1501 cctgcaagcc gcgcatgacc attgtcgcgc gtggatcgat ctgacgagta aagtgcaaat
     1561 tattggggtt tattgttcac aattccatgg atgatcgaat cttctccaat ttttttgtgc
     1621 ccgagttggg ccgcagccga gcgattgcac gccgttatgg caacgtcccc gggatttggc
     1681 tggtaatcct ggccgcattc gctgtgcaag gatgcaacag gtccgatgaa gaacagcgag
     1741 cccggcgaat ggcctcacgt gtggaattag aatcggtgat tcggatgaac aacctgaata
     1801 attatccgga ctgatcttta ctttcggcct taactccgac ggttgaagcc gctccgaata
     1861 tttcggagcg gctttctttt tgaatcgtgc cgtcttttca aaaagctctt gactggatcg
     1921 ctcgcctaat tatataagtg cttatatgtc tgcaagcctt cccgccggct tgggaaccaa
     1981 gctccggcaa ctgatcaccc gactcgatgg cgatgtgcag caactctatg gcgaaatgaa
     2041 cgcgcctttc cggccccgtt tttatcccgt agttcgtctc ctgcttctaa aacctcaaac
     2101 cgttaattcg atcgcagcac aggtcggagt cagtcagcca gccattaccc aaacgctcgg
     2161 cgagatgaga aagattggac tgattgcggc gaaaccggga tccgatcggc ggaagcaaat
     2221 cattcacctg acccggaaag gcgaatcggt agcggagaaa ttgcagccgg tttggaacgc
     2281 cacgcatcgg gcggcggccg gtctcgatgc ggacttagcc gcccggctcg gcgaaatcgt
     2341 cgaccaagcg ctcgctgcgc tggatgagag accgttcaaa gagcgaatcc gcattcaact
     2401 cagcacaaaa aatcatgact agacttctca tcagcgcgct tctcgcaggg tatgccacgg
     2461 caacggccct ttcgcctaac gaccaatccg aagtggtacg aagtgctgcg cgggtgatcg
     2521 aaaagcgtta cgtcgatccg gagcgcgctc acgaaatcgc acgcaagctt cgcgacgcgg
     2581 aatcgcgctg gagccacgtc accgatccga aatcgtttgc tggcgccgtc accaaatggt
     2641 tgcgcgagat ttcgggcgat ggacatctgg gcctcagcta tagcgagaaa gtattgccgg
     2701 aacagactgg cgacgagagc ttctccacgg ccgagatgga gaaatattac ggcgcccatc
     2761 tcaatcatgg catcgagaaa atcgagcgcc tccccggcaa cattatcctg ctcgatatcc
     2821 gcgtctttcc gccaagcccg atggcgggag atgtatttgc ggcggtcatg acggtggtcg
     2881 cgcaaggaga tgcgttgatc atcgatttga gaaagaatgg cggcggcgct gagacagcga
     2941 acctcgtcac cggttatctc ctcgatggcg gcaaaccgtt gagtggcgac tataatcggc
     3001 ccaccgacag acactccgct aacatctcgc cctcttgggt gccggggcgg cggttcggct
     3061 ctaccaagcc cctctatatt ctcacttcgc ggaagacgtt ttccgcggcc gaagccatcg
     3121 cctacgatct tcaggcgttg aagcgcgcaa ccatcgtcgg agaagtcacc ggaggtggcg
     3181 cgcatccctt cgaatatcga cgggtgcatc cgcattttgc cgtggatctg ccggaaggtc
     3241 gttcgatcaa tccgattacc aaaacgaact ggcaagacgt aggcgtgcag ccggacatcc
     3301 cggtgccggc tgagcaggca ttggacaaag cattggaact tgcgcgcgcc gcgatccgga
     3361 aaaatcgata agccaataaa tggagccggc agtcggattc gaaccgacga cctgctgatt
     3421 acaaatcagc tgctctacca gctgagctat tccggcgctt ttatctcggc aaaccgcctc
     3481 gtggcgatcc cgaggattgg gcaagtgtat agcacgattt ccgccgttct gctgaagcaa
     3541 atttcgccga caaataaaat aaccgatctg cggcgtcgaa gggattcacg gttggcgcgg
     3601 cggtacgatc aatggcgtct tgaccggcgc tgcgagtcca ataaacacga agagtccgtc
     3661 gcctcctggg tttgtctgga cgggatctat ccatccatga atgaacgttc gggacaaaaa
     3721 gattggaaag aattatctgc tgtcgtgaaa aatctcgaat cgatgagcgt caaaccgatt
     3781 cgcggcagtc cggcaactaa acctacacgt cgcacgcttg tattcgcgat ttcgctggta
     3841 atcctggcag cattcgctgg gcaaggatgc aacaggtccg gcgaagacgc gcaggcgcgg
     3901 cgaatggccg caagtgtgaa ggcagaattt ctgcatgcat ggcgcaatta cgagcgctat
     3961 gcgtggggac atgacgcatt gcagcccttg agtaaaaccc cacacgattg gtacgagcag
     4021 tcgctcttga tgaccccagt cgatgcgctc gacacactcg ttttgatgaa tctcgatgcc
     4081 gacgtcgcga agacgaagga gct
//
LOCUS       DASQUQ010000260         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_976406, whole genome shotgun sequence.
ACCESSION   DASQUQ010000260 DASQUQ010000000
VERSION     DASQUQ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976406"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="xseA"
                     /locus_tag="VE486_07220"
     CDS             complement(<1..838)
                     /gene="xseA"
                     /locus_tag="VE486_07220"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZA38917.1"
                     /translation="MQLTDFFQQTSKVFTVAELTRQIRGMLETKFGAVWVQGEVSNYK
                     LHPSGHQYFTLKDARAQIACVIFRNTMPPLRTPLADGAQVQVYGSVSVFEARGQYQLS
                     VQILQPRGLGLLQAKFEALKRKLEAEGLFDAARKRALPKFPRRIGIVTSPSGAAIRDI
                     LNVLKRRAPWLQILINPVRVQGTGAAQEIAVAIRQLATPNVNWRPLDVVVITRGGGSI
                     EDLWEFNEEIVARTIADVRVPVVSAVGHEIDFTICDFVADLRAPTPSAAAELIVPDVI
                     DLH"
     gene            1296..>2471
                     /locus_tag="VE486_07225"
     CDS             1296..>2471
                     /locus_tag="VE486_07225"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZA38918.1"
                     /translation="MNAEKPFNFEETPGKLPKTVVPVEYSVRIVPSIDNFTFTGTEEV
                     KLSVRRPVRQLVLNALELEVADASIDGQPLPGSAIKIDKKNELLTLALPSELAAGEHT
                     LALSFAGKINQQGQGLFYVPYQEHGSGAKKVLLATQFEATDARRFFPCWDEPVFRARF
                     QLTAVVPENWLAVSNMPIESEKKIADGKEVRFAVSPPMPSYLNVFVAGELDLIESHSG
                     PTQLRVIATKGKAEMGHYALEATAQILKYYNDYFGVPYPLPKLDQIALPGGFGGAMEN
                     WGGITYYESTLLFDPKNSSAETKQNIYEVIAHEMAHQWFGNLVTMAWWDNLWLNEGFA
                     TWMGTKCTAHFNPQWEVWLRRNLPRDPTRRVGIAREQAMEGDARSTTHPIQQPIATEA
                     "
BASE COUNT          566 a          671 c          725 g          509 t
ORIGIN      
        1 gatgaagatc gataacatcc ggcacaatta attccgcggc agcgcttggc gtgggcgcgc
       61 gcaagtcggc gacgaaatcg cagatggtga aatcgatttc atgtccgacc gccgagacga
      121 ctgggacccg aacatcggcg atcgtgcgcg cgacgatttc ttcgttgaat tcccacaaat
      181 cttcaatgct gccgccaccg cgtgtgatca caacgacgtc gagtggtcgc caattcacgt
      241 ttggggtggc cagttggcgg atcgcaacgg cgatctcctg cgcggcgcca gtcccctgta
      301 cgcggactgg attgatcaaa atttgcagcc acggtgcgcg ccgtttcaag acgttgagaa
      361 tatcgcgaat ggccgccccg ctgggcgacg tgacgatacc aatacgtctc ggaaatttcg
      421 ggagcgcgcg cttgcgggca gcgtcgaaaa gtccttccgc ttccagtttg cgcttgagcg
      481 cttcgaattt tgcctggagc aggccgagac cgcgaggttg caagatttgc acgctcagtt
      541 ggtattgccc tcgcgcttcg aagaccgaga cactgccgta aacctgtacc tgagcgccgt
      601 cggcgagcgg agttcgcaat ggcggcatcg tattgcggaa gatcacgcaa gcgatctgcg
      661 cccgggcatc tttgagcgtg aaatactggt ggcccgaggg atgcagcttg taattggaca
      721 cttcgccctg aacccagacg gcgccgaact tggtctcgag catgccgcgg atctggcgcg
      781 tcaactcagc gacagtgaaa actttggaag tctgctgaaa gaaatcggtc agctgcatgt
      841 cgattgagcc taagagacgc tattatttaa cagttagaga ttcctcgact ccgctcggaa
      901 tgacaaatga gaaaagcgtt cgttggctcg gtccgagtat gagcaagagc aagagtaatg
      961 acgacccgca cacgaaacgc ggacttcagc ttgatcgcgt cgtgttgttt tcgcggcaaa
     1021 gaaacaccgg aagccggttt tcaagttagg gttgcggctc cgccgcatct gtctcagacg
     1081 ccgaggaaag tgggcgtttc acggaatgga gccccgggga cgcgagcgac atggacgggc
     1141 agctcgtgag agcgacagag cgggagcgaa cgagtcaaac gccccacaag tccgcctttg
     1201 cagatgcaaa cttcggtgca cgttgtttaa tgcgggaaat gcgaacgggg cttctttttt
     1261 tcgctggtct attattcatt atcgtctgcc ccgacatgaa tgccgaaaag cctttcaact
     1321 ttgaggaaac gccggggaaa ctgccgaaaa ctgtcgtccc tgtagaatat tcggttcgga
     1381 ttgtccccag tatcgacaac ttcaccttca ctggaacaga agaggtgaag ctcagtgtgc
     1441 ggcgaccggt tcgtcagctc gtgctgaatg cgctggaact cgaagtcgca gacgcatcga
     1501 tcgatggcca gccgctcccc ggctctgcga ttaaaatcga caagaaaaac gagctgctca
     1561 ccctggcgtt gccatcagaa cttgcagccg gtgagcacac gctcgcgctt agcttcgcgg
     1621 gcaagatcaa ccagcagggg caggggctgt tttacgtgcc ctatcaggag cacggcagcg
     1681 gcgcgaaaaa ggtcctgctc gcaacgcaat ttgaagcaac cgatgcccgc cgttttttcc
     1741 cttgttggga cgaaccggtt tttcgcgcgc gctttcaatt gacggcggtt gtgccggaaa
     1801 actggctggc cgtgtcgaat atgccgatcg agagcgaaaa gaaaatcgct gatggaaaag
     1861 aggtgcgctt tgccgtgagt ccgccgatgc cgagttatct gaatgttttc gtcgcgggcg
     1921 aactggatct catcgaatcc catagcggcc ccacgcagct tcgtgtcatc gcgaccaagg
     1981 gcaaagcgga aatgggtcat tacgcgctcg aagccaccgc gcagatcctg aaatattaca
     2041 atgattattt cggcgtgcct tatccgctgc cgaagctgga tcaaatcgct cttcccggag
     2101 gcttcggcgg tgcgatggag aactggggcg ggattacgta ttacgaatcc acgcttctat
     2161 ttgatccgaa aaactcctct gctgagacga aacaaaatat ctacgaagtg atcgcgcacg
     2221 aaatggcgca ccagtggttc ggcaatctcg tgaccatggc ctggtgggac aacctgtggc
     2281 ttaacgaagg gttcgccacc tggatgggca cgaaatgcac ggcccatttc aacccgcaat
     2341 gggaagtctg gctgcgcaga aatttgccgc gcgatcccac gcgacgcgtc ggcatcgcca
     2401 gggaacaagc aatggaaggc gatgccagat cgacaacgca tccgattcag cagccgatcg
     2461 cgaccgaggc t
//
LOCUS       DASQUQ010000261         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_68661, whole genome shotgun sequence.
ACCESSION   DASQUQ010000261 DASQUQ010000000
VERSION     DASQUQ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68661"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VE486_07230"
     CDS             <1..226
                     /locus_tag="VE486_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38919.1"
                     /translation="GGNEWEALTKGLPQRNCYVNVLRDAMSVDSLEPCGVYFGTTGGQ
                     VYASADAGNNWKPIVRDLPAVLSVEVQTLK"
     gene            437..733
                     /locus_tag="VE486_07235"
     CDS             437..733
                     /locus_tag="VE486_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZA38920.1"
                     /translation="MIRVVLPPHLRTLARVDGEVQLDVDGAVTQRSVLDALETRYPML
                     CGTMRDHVTQLRRPLVRFFACGEDLSHESPDARLPDTIANGSEPFVIIGAIAGG"
     gene            873..1253
                     /locus_tag="VE486_07240"
     CDS             873..1253
                     /locus_tag="VE486_07240"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZA38921.1"
                     /translation="MNTTEEVAKKLVELCRNGEWRKALDELYAKDIVSVEAQEMENMP
                     AEMRGIDQVRGKTDWWEKNMEVHSAKVGGPFVARDTFVVQFDIDVTDKESKKRMQLSE
                     VGIYTVKDGKVAREEFLPLAQKKE"
     gene            complement(1332..>2972)
                     /locus_tag="VE486_07245"
     CDS             complement(1332..>2972)
                     /locus_tag="VE486_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZA38922.1"
                     /translation="GYLIAGVVVGPYTPGVVGDAKIASQLAEIGVMLLMFGVGLHFSL
                     KDLLAVRALAIPGALIQSTAATVACIGLAVAVGWSWRSGLILGIAVSVASTVVMLRAL
                     LDQGIVNTAEGHIAIGWLVVQDIVTVLVLVMVPALAGASGASNMSHTVLVAVAKLALL
                     VAIMMLAGARFVPWLFLRVARLRSAELFTLAVLVMAICVATVSYMVFGASMALGAFLG
                     GMVVGQSKVSDQAAADVLPMRNVFAVLFFVSVGMLFDWRAVIALPALLIGVLGIIFLV
                     TPLVALLVVIVSGHAVKTALTLAAGLAQIGEFSFIVAQMAKTLGLMPASGHNVIVGAA
                     IVSISLNPLIFRWVLALEPRLSKLPWLVTRSEKLGARANVIAVKRKQEDPGAIVVGYG
                     PVGQTVTRLLSEFGINPMIVETNVDVVVELQQRGKQALFGDASRPDILRAAGLDTAAY
                     LIVTVPHAEISLRIIQAAREVAPLVRIITRAEYINQSDAFLQAGAAIIRYDEAESAAA
                     LAEALLQDIDVPADRIDALVSSIRNELAPARKSLTIES"
BASE COUNT          723 a          843 c          854 g          552 t
ORIGIN      
        1 cggtgggaac gaatgggaag cgctcaccaa aggcctgcca caacgtaatt gctacgtgaa
       61 tgtgctgcgt gatgccatgt cggtcgattc gctcgagccg tgcggcgttt attttggcac
      121 gactggcgga caggtttacg cgtcggccga tgcgggcaat aactggaaac caattgtgcg
      181 agatttgccc gccgtactgt cggtggaagt gcaaacgcta aaatgaaatc ccaaatccaa
      241 atcccaaaca gcagacgaat gacgaaatcc gaatgacgaa gctcgaaaga atgacgaatg
      301 ataaaatgtt aaggaattgt ggcgctgctt ctttgtcatt ggcgtttcgt gcttctttcg
      361 tcattcgaca ttcgtgcttc gtaatttctt gccgacgttc cgatttgttt cgagtttcga
      421 atttaggatt tgaaatatga ttcgcgtagt tctcccgccc catctgcgta cgctcgcgcg
      481 cgttgacggc gaagtgcaac tcgacgtcga tggtgcggtc acacagcgat cggtcctcga
      541 cgcgcttgaa acccgatacc cgatgctgtg tgggacaatg cgcgatcatg tgactcaact
      601 ccggcggcca ctcgtgcgat tctttgcctg cggagaagat ctatcgcatg aatcgcctga
      661 tgctcggctg ccggacacga ttgcaaacgg atctgagccc tttgtaatca tcggcgccat
      721 tgcgggcggt taatcgagcc cgttgctttt cgccttcgcg gttgacgaga gtgttcggcg
      781 ctcatagacc accgctacag agtggcaaca agtcacaatg atgttacaga aatatcggtt
      841 gaacaattct aaatcaaaaa taaggaagaa gaatgaatac tacagaagaa gtcgcaaaaa
      901 aactggtgga gctttgccgc aacggcgaat ggaggaaggc gctcgatgaa ctctacgcga
      961 aggacatcgt cagcgtggag gcacaggaga tggagaacat gccggcggaa atgcgcggca
     1021 ttgatcaagt acgcggtaag actgactggt gggagaagaa catggaagtg cacagcgcga
     1081 aagtcggcgg accgtttgtg gcccgcgata cctttgtcgt gcaattcgac atcgatgtga
     1141 ccgacaaaga gtccaagaag cggatgcaac tgtccgaggt aggaatttac accgtcaaag
     1201 acggcaaagt cgcccgcgag gaatttctgc cgctcgctca aaagaaagaa taacaaggaa
     1261 cagcacgagg ctctggtccg cccaatcttg cgttgagctt atcaaaacgc ggtaatgcgt
     1321 ggccgctgtg atcagctttc aatcgttaga cttttccgtg ccggagcgag ctcgttgcgg
     1381 atgctactta ccagcgcgtc gatgcgatcc gcgggcacat cgatgtcctg cagcagagcc
     1441 tcggcgaggg cagcggcgga ttcggcttcg tcgtagcgga taatggccgc gccggcctgc
     1501 aaaaaggcgt cggactgatt gatatactcg gcgcgggtga tgatccgaac cagcggcgcg
     1561 acttcgcgtg cggcttgaat gatgcgcaag ctgatctcag cgtgcgggac cgtcacgatg
     1621 agataggctg cggtgtcgag tccggcggcg cgcaggatat ctggtcggga cgcatctcca
     1681 aacagcgcct gcttgccccg ctgttgcagc tccacgacga cgtccacatt tgtctcgaca
     1741 atcatcggat tgattccgaa ctcggacagc aagcgcgtaa ccgtttgacc aacaggtccg
     1801 tagccaacca cgatcgcgcc cggatcttcc tgtttacgtt tcaccgcgat tacattggcg
     1861 cgcgcgccaa gtttttcgct gcgagtcacg agccagggca atttcgaaag ccgcggctcc
     1921 agcgcgagca cccagcggaa gattagaggg ttcaggctga tcgagacaat agccgcgccg
     1981 actattacgt tgtggccgct ggccggcatg aggccgaggg ttttcgccat ctgggcgacg
     2041 atgaaggaaa attctccgat ctgcgcgaga ccggccgcca gcgtaagggc tgttttcacc
     2101 gcgtgcccgc tgacaatcac gacgagcaag gcgactagcg gcgtaaccag aaaaatgatg
     2161 cccaatacgc ctatgagcag ggccggcagc gcgatcaccg cccgccaatc gaacaacatc
     2221 ccgaccgaga cgaagaagag taccgcaaag acgttacgca tcggcaaaac atcagcggcg
     2281 gcctgatcgc tcaccttcga ttgccccacg accatgccgc cgaggaatgc accgagcgcc
     2341 atcgaagcgc cgaacaccat gtaggagacc gtcgccacgc agatggccat caccagcacg
     2401 gcgagcgtga acagctcagc ggaccgaagt cttgcgacac gaagaaacaa ccacggaacg
     2461 aaacgcgcac cggccagcat catgatcgcg acgagcagcg caagcttcgc cactgcaacc
     2521 aggacagtgt ggctcatatt cgacgcgccg ctggcgccag ccaaggcagg caccattacc
     2581 agcacgagaa cggtgacgat atcctgcacg accagccagc caatggcgat gtgtccctcc
     2641 gccgtgttca cgatcccctg atcgagcaat gcgcgcagca tgaccaccgt gctcgcgaca
     2701 gacaccgcaa tgccgagaat caaaccgctc cgccagctcc aaccaaccgc aacggcaaga
     2761 ccaatgcagg cgacagtcgc ggcagtgctt tggatgagag cgccgggaat cgcgagcgcc
     2821 cgcaccgcga gcaggtcctt cagactaaaa tgcagaccga cgccgaacat cagtaacatc
     2881 acgccgatct cggcaagctg ggacgcaatc ttcgcgtcac caaccacgcc tggcgtgtag
     2941 gggccaacga cgacaccggc gattaggtag cc
//
LOCUS       DASQUQ010000262         3271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1155417, whole genome shotgun sequence.
ACCESSION   DASQUQ010000262 DASQUQ010000000
VERSION     DASQUQ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155417"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /gene="sppA"
                     /locus_tag="VE486_07250"
     CDS             <1..998
                     /gene="sppA"
                     /locus_tag="VE486_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZA38923.1"
                     /translation="DRRLGCLSIFLFVALCASLFVNFVLMAAVFQRYGGAREIEPLPR
                     FREVLLQRGARGTLDKVAVITMRGLISSSVPGTVSDSMVDDMRAAFQQARDDNRVKAI
                     VLEIDSPGGEVTASDAIYSAVVKARAKKPVVVYMESLAASGGYYVSCGGKFLMASDTT
                     ITGSIGVIIQTLNYEQLLNKVGLASVVFKSGKFKDMLNGARPITPEERELVQSFIMKT
                     YDKFLGIVAKERNLSADLLRSSIADGRILSGKDAFENKLIDGLGELDDAFAKAKQLGN
                     APGAKIVKYGPPFSLSRFLRIFGEADSKIELQLPKQFVPQLESGRAYFLPSYYAP"
     gene            1008..1841
                     /locus_tag="VE486_07255"
     CDS             1008..1841
                     /locus_tag="VE486_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HZA38924.1"
                     /translation="MPSDGFFATFGAAPTYVVALFSLLYLLAGIYIYISLIRQIAARA
                     SVPAGEPAKAFGLPEAILAGVLILFLLLSITASVSRPSIDLSARNLLANLLFTMFIVL
                     FIVTFLQLRGFDLSSLAGFFKIGFVRALGTGIILLLAAYPLITLSNAIAQHIFGSGPG
                     KQNIVELFSGSRTIEQRIMIIVFAVAIAPVVEEFFFRFFLYGVIKQYLGRFLGIAVNA
                     LLFAAAHTHFPSFVPLFVLGSCFTIAYEWSGSILVAMTMHSLFNSVTLIVLAFPELFS
                     Q"
     gene            1838..2737
                     /locus_tag="VE486_07260"
     CDS             1838..2737
                     /locus_tag="VE486_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZA38925.1"
                     /translation="MKLREVGEDRLLDQLLPRLQLGKTVVAGAGDDCAVIEFFAGRNF
                     LVLKTDCIVEGVHFLSRANPVDVGWKAMMRPLSDFAATSALPQFALITLIASKQTEVE
                     WVRKLYRGLRRAAKRFGVSIVGGETSRTPGPIVISVNVAGFVEKDRWVSRRGGKVGDD
                     LFVTGQLGGAVRQKHLSFIPRIAESRWLTENFSIHAMMDLSDGLGVDLPRLARASKVG
                     FKIEMEKLPLTRGAEIGNAISEGEDYELLFAISPRDRKGLKRAWRKKFPKLPLTRIGR
                     LNRKSKIENRKLPRGYVHFQQPG"
     gene            2706..3134
                     /gene="tsaE"
                     /locus_tag="VE486_07265"
     CDS             2706..3134
                     /gene="tsaE"
                     /locus_tag="VE486_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZA38926.1"
                     /translation="MATFISNSPAETEAIGQQFAVKDVGVGSILALKGELGSGKTLFT
                     KGLVAGLGSAATVTSPTFTIVHEYPGGRLPVYHFDFFRLEDRQSVARLGLDDYLFGDG
                     VSVIEWAERFPEFIPEQARWILFEIRSENERAITLDDDRL"
BASE COUNT          670 a          866 c          914 g          821 t
ORIGIN      
        1 cagatcgcag gctcggttgt ctcagcattt ttttgtttgt cgcgctttgc gccagtctgt
       61 ttgtgaactt tgtgctgatg gcggcggtgt ttcagcgtta tggcggggcc cgggaaatcg
      121 aaccgcttcc gcgtttccgt gaggtcctgc ttcaaagagg cgcccgcgga acattggaca
      181 aagtcgctgt catcaccatg cgcggtttga tcagctcttc ggtcccgggc accgtgtccg
      241 attcgatggt tgatgacatg cgggcggcgt ttcaacaagc gcgtgatgac aatcgggtca
      301 aagccattgt gctggaaatc gattcgcccg gcggagaagt gaccgcttcc gatgccattt
      361 acagcgctgt ggtcaaggcg cgcgccaaga aaccggtcgt ggtttacatg gaatcgctcg
      421 cggcttcggg cggttattac gtttcctgcg gcggcaagtt tttgatggcg agcgacacga
      481 cgatcaccgg cagcatcggc gtgatcattc aaacgctgaa ctatgagcaa ctgttaaaca
      541 aagtgggact ggcttccgtt gttttcaaaa gcggcaagtt taaggacatg ctcaacgggg
      601 cgcgaccgat tacgccagag gagcgcgagt tggtccaaag ttttattatg aaaacctacg
      661 ataagtttct cggcattgtc gcaaaggaga gaaatctttc ggccgatctg ctgcgcagtt
      721 ccatcgccga cggccgaatc ctctcgggga aggatgcgtt cgagaacaag ctcatcgatg
      781 ggcttggtga attggacgac gccttcgcca aagccaagca attgggaaat gcgccggggg
      841 cgaaaattgt gaagtacggg ccgccattca gtttgagccg attcttgcga atatttggag
      901 aggctgactc aaaaatcgaa ttgcagcttc ccaaacagtt cgtgccacaa ttggaatcgg
      961 gacgtgcgta ttttctgccg agctattacg cgccgtaaaa aagaggcatg ccatccgacg
     1021 gctttttcgc gacgttcgga gccgctccga cctatgttgt agctctcttt tcgctgctct
     1081 accttctcgc cggaatttat atttatatct cgctgattcg ccagatcgcc gcgcgcgcgt
     1141 cggtccctgc cggtgagccg gcgaaggcct tcgggctgcc cgaagcaata cttgccgggg
     1201 tattgatcct tttccttctg ctcagcatca ccgcctcggt ttcacgcccc tctatcgacc
     1261 tcagcgcgcg aaatcttttg gcaaatctgc ttttcacgat gttcatcgtg ctgttcatcg
     1321 tgacttttct gcagcttcgc ggatttgatc tcagctcgtt agccggtttt ttcaaaatcg
     1381 gttttgttcg agccctgggc acagggatca tcctgttgtt ggctgcttat cctttgatca
     1441 cactgtccaa cgcgattgcg caacacattt tcggcagcgg gccaggcaag cagaacatcg
     1501 tcgaactttt cagcggttcg cgcacgatcg agcaacgaat catgatcatt gtgtttgcgg
     1561 tggcgattgc tccggtggtt gaggaatttt ttttccggtt tttcctttat ggtgtaatca
     1621 aacaatatct cggccggttt ctcggaattg ccgtgaacgc gctcctcttc gccgcggcgc
     1681 acacacattt cccttcgttc gtgcccctgt tcgttttggg aagctgtttc acgattgcat
     1741 acgaatggag cggctccatt ctagtcgcga tgacgatgca ttcgctgttc aactccgtga
     1801 cgctcatcgt gctcgctttc ccagaactct tctcgcaatg aagctgcgcg aggtcggcga
     1861 agacaggctg cttgatcaac tcctgcctcg tctgcagctg ggaaaaacgg tcgtcgcagg
     1921 cgccggcgat gactgtgctg tgatagaatt ttttgcaggc cgcaactttc tcgtgctcaa
     1981 aaccgattgc atcgtcgagg gcgtgcactt tttgtctaga gcgaacccgg tcgatgttgg
     2041 ctggaaggca atgatgcgac cgctcagcga ttttgccgcg acctcggccc tgccgcagtt
     2101 tgcgctgatt acgctgattg cttcgaaaca aacggaagtc gagtgggtga gaaaactcta
     2161 tcgcggcctc cgccgtgcgg caaagcggtt cggtgtcagc atcgtgggcg gcgaaacgag
     2221 cagaacaccc ggtccaattg ttatttccgt caatgtcgct ggattcgtcg agaaagaccg
     2281 ctgggtgtcg cgccggggcg gcaaagttgg cgatgatctt ttcgtcactg gccagctcgg
     2341 tggcgccgtt agacaaaagc atttgagttt cattccgcgg atagcagagt cgcgctggct
     2401 cacggaaaat ttctccatcc acgccatgat ggatttgagc gatggactcg gtgtagacct
     2461 tccgcgcctc gcgcgtgcca gcaaagttgg gttcaagatt gagatggaaa aattgccgct
     2521 tacccgcggc gcagagatcg gcaatgcaat ctcggaaggc gaagattacg aattgctctt
     2581 tgccatttcc ccgcgtgaca gaaagggtct gaaacgagcg tggcgaaaga aattcccgaa
     2641 actgccgctc acccgcatcg gtcgcctaaa tcgaaaatcg aaaatcgaaa atcgaaaatt
     2701 gcctcgtggc tacgttcatt tccaacagcc cggctgaaac cgaggcaatc gggcagcaat
     2761 tcgccgtgaa agatgtcggg gttggatcga tcctggccct caaaggcgaa ctgggaagcg
     2821 gcaaaactct tttcaccaaa ggtctcgttg ccggactggg cagtgccgca acagtcacca
     2881 gccctacgtt taccattgtc cacgaatatc caggtggccg cttgcccgtt tatcatttcg
     2941 atttctttcg gctggaagat cggcaatcgg tcgcgcggct tgggctcgac gattaccttt
     3001 ttggcgacgg cgtttcggta attgaatggg ccgagcggtt tccagaattc attcccgagc
     3061 aggcgcgctg gatcttgttt gagattagat cagaaaacga gcgggcgatc acgttggatg
     3121 atgaccgatt atagcccagc gctttagcgc tgggtcacgc gatgtttacg tcatcaattc
     3181 ccggaaggga cggaggaaat tcacggagac cgctacgctt cttctttcgt ccctgacggg
     3241 actcgtcatc tctctttcct gctgcccagc g
//
LOCUS       DASQUQ010000263         3382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_347725, whole genome shotgun sequence.
ACCESSION   DASQUQ010000263 DASQUQ010000000
VERSION     DASQUQ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347725"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VE486_07270"
     CDS             <1..1455
                     /locus_tag="VE486_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZA38927.1"
                     /translation="LVQQLLTEGVLLSLAGGALGVLLAAGINELVAGIKLPTDIALVF
                     DLHLDWRVLLFAMLLSILTGVVFSLLPALQSSKPALVPSLKDDAKLGGFRRSRLRNSL
                     VVAQVSLSLVLLVCAGLIVRSLQAAQQMRPGFDTHNAVAISFDVGLQGYDEAKGRSFQ
                     RQILERARAIPAVRAAALTSSLPLALNYNSAGVYLEGQPATSASNLPLCVPKSVSPGF
                     FDTMGIPFRGRDFAENEDKKESRVAVVDETFARKFFPGQDPIGKRFNFTGPKDPFWEI
                     IGVAGNIKHNSLAETPQPSVFRPQFRDYDTVVTLVARTRSDPRSVIAALRKEFQAMDP
                     TLPLYDIKTLTEHMKIPLFPARMAANVLGTFGVLALVLAAIGIYGVMSYVVAGRTREI
                     GLRMALGAQLRHVRQLILGQGMILASIGLAVGLVIVFILARFLTSILYGVSPTDPVTF
                     IAITFLLAGVALVACYIPARRAARVDPMVALREE"
     gene            1505..1915
                     /locus_tag="VE486_07275"
     CDS             1505..1915
                     /locus_tag="VE486_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZA38928.1"
                     /translation="MPQTITGGCACGAIRYEINADPIVMFNCHCRDCQRTSGGPYTPV
                     FYVPAKAFRITKGSPHYYGTTSEMVGHNKRGFCPECGSRLFGGATEQGQGITASSLDD
                     PTLFKPQFEIWTSDAQPWDHMDPNVPKHEKYAPS"
     gene            1930..2745
                     /locus_tag="VE486_07280"
     CDS             1930..2745
                     /locus_tag="VE486_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HZA38929.1"
                     /translation="MMQSLTLRFIFLGFVGTHLHIPLWVALAGEAKSGIVTLDAKPEP
                     IAIDPGRTAVIVVDMENDFAAKGGMFDRAGIDISGAQNAIAPTARVLAAARQAGLKVI
                     YLKMGYRPDLSDLGATDSVNRTRHLKFGVGQKVQAPDGRESRVLIRDTWNTDIVPELK
                     PQTSDIVIYKTRFSGFYQTDLDATLKKFGIKYLIVTGVTTSICVESTVRDAMFRDYLC
                     VLLRDCMSEPIGHDLARTNHEASLLNAQTLFGWVSDSDQFIKALAAKASQALR"
     gene            2805..>3382
                     /locus_tag="VE486_07285"
     CDS             2805..>3382
                     /locus_tag="VE486_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HZA38930.1"
                     /translation="MRTGNNVSETDPFDLLRQIAGGYCVSRAVHVLAELNVADALDDT
                     PQSAAELAKSTGADPDALYRILRLTSAHGIFQTEGDRFKHSPASRMLRSDHPQSMRSF
                     VRMFGLANNWSAYGELEHSVRTGRPATEKTLPEGFWTYLAQAPEANSIFNETMLAKAR
                     GQIPAIVASYDFSQFDVIADIAGGRGHLISAIL"
BASE COUNT          722 a          950 c          929 g          781 t
ORIGIN      
        1 cttgttcaac aactacttac cgaaggtgtg ttgctttcac tggccggtgg cgcgcttggc
       61 gtgttgcttg cagccgggat caatgaactt gtcgctggca tcaaactgcc gacggacatc
      121 gcccttgtgt tcgatctgca cctcgattgg cgagtattgt tgttcgcgat gctgttgtcg
      181 atcctgaccg gcgtcgtctt cagtctattg cccgctttgc aatcttcgaa acccgcgttg
      241 gtgccgagcc tgaaagacga cgccaaactc ggtgggtttc gccgttcgcg cttgcgcaac
      301 tctctcgtcg tcgcgcaagt ttccttgtcg cttgtccttc ttgtttgtgc cggattaatc
      361 gtacgcagcc tccaagccgc gcaacagatg cgtccgggat tcgataccca caatgccgtc
      421 gcgatttcct ttgatgttgg attgcaaggg tacgacgaag ccaaaggccg ctcttttcaa
      481 cgtcagatcc tcgaacgtgc ccgcgccatt cccgcagtac gcgcggctgc gctaacaagc
      541 agccttccgc tcgcgcttaa ctacaactct gccggcgttt atcttgaagg ccagcccgcg
      601 accagtgcca gcaatttgcc cttgtgcgtt cccaagtcgg tcagcccggg cttttttgat
      661 acaatgggaa ttccgtttcg cggccgcgat tttgcggaga atgaagataa aaaggaatca
      721 cgtgtcgctg ttgtagatga gactttcgcg aggaagtttt tccccgggca ggatccgatc
      781 ggcaaacgat tcaatttcac cggaccgaaa gatccattct gggagatcat cggtgtcgcg
      841 ggcaatatca agcataacag cctggctgaa acgccacagc cgtcagtctt tcgcccgcag
      901 tttcgcgact acgatacagt cgtcacgctg gtggcgcgca cccggagcga cccacgctct
      961 gtgatcgccg cgctgcgcaa agaatttcag gcgatggatc caacgcttcc gctttacgat
     1021 atcaagacat tgaccgagca catgaaaatc ccgcttttcc ctgctcgcat ggccgcgaac
     1081 gtactcggga ctttcggcgt cctcgcgctc gtgctcgcag ctatcggtat ttacggagtg
     1141 atgtcgtacg tcgtggctgg acgaactcgc gagatcggtt tgcgcatggc gctaggcgcg
     1201 caactgcgac atgtccgtca gctcattctt ggacaaggca tgatcctcgc cagcatcggt
     1261 ctcgccgtcg gcctggtcat cgttttcatt ctcgcccggt tcctgaccag catcctttac
     1321 ggcgtcagcc caaccgatcc ggttactttt attgcgataa ctttcctgct cgccggagtc
     1381 gcgttagtcg cctgctacat tcccgcccgc cgcgctgcgc gtgtcgatcc catggtcgcc
     1441 ttacgcgaag aataaaggtg agctaatcgt ttgaaagctc ccgcggatgc atgaatggtt
     1501 caccatgccc caaaccataa ctggcggatg cgcgtgcggc gcgattcgct acgaaatcaa
     1561 tgcggacccg atcgtaatgt tcaactgtca ctgccgcgat tgtcagcgga caagcggcgg
     1621 tccgtacact cccgtttttt atgtgcccgc gaaggcgttc aggatcacga aaggatcgcc
     1681 gcattattac ggcacgacca gtgagatggt tgggcacaac aagcgcggtt tttgtcccga
     1741 atgtggttcg agacttttcg gcggcgcgac tgagcaaggc cagggcatca ccgcgtcgag
     1801 cctggatgat cccactttgt ttaaaccaca gtttgaaatt tggacgtccg acgcgcagcc
     1861 ttgggatcat atggacccaa acgtgccgaa gcacgagaaa tatgctccga gttaacctaa
     1921 cgtgaagcca tgatgcaaag cctaacgctc agattcattt tcttaggatt cgttggcaca
     1981 caccttcata ttccgttgtg ggtagctttg gctggcgagg cgaagagtgg aattgttacg
     2041 ctcgatgcca agcccgaacc gatcgcaatc gatccgggcc gaaccgcggt cattgtcgtc
     2101 gatatggaga acgatttcgc tgccaagggc gggatgttcg accgggccgg aatcgatatt
     2161 tccggcgcac agaacgctat cgctcccacg gcaagggtgc tggcggcagc gcggcaagcc
     2221 gggttgaagg tcatttatct caagatgggt taccgaccag acctttccga tctaggcgca
     2281 accgattcgg tgaatcgcac gcggcactta aaatttggag tcggccagaa agttcaggcg
     2341 ccggacggac gcgaaagccg tgtgttaatc cgagacacct ggaacaccga catcgttccc
     2401 gagttgaagc cgcagaccag cgatattgtc atttataaaa cgcgattcag cgggttctac
     2461 caaaccgatc tggacgcaac gctgaaaaag tttgggatca aatacctgat cgtcaccggc
     2521 gtaacgacaa gcatctgcgt ggaatcgacg gtgcgagacg caatgttcag agattatttg
     2581 tgcgttctgc tgcgagattg catgagcgag cccatcggcc acgatttggc gagaacgaat
     2641 cacgaggcct ctcttttaaa tgcccagaca ttgtttggat gggtttcaga ctcagatcaa
     2701 ttcatcaagg ctttggccgc aaaggcgtcg caagccctgc gatgaacgaa tctttccaaa
     2761 gttctcgcgc gcctgaacct gaagtaaatc ctgtaacagc atcgatgaga accggaaaca
     2821 atgtgagtga aactgatcct tttgatcttt tgcggcaaat cgcgggcggt tactgcgttt
     2881 cacgggcggt tcatgtgctc gcggaattga acgtagccga cgccttggac gacacgccgc
     2941 aatccgccgc cgagctggca aaatcgacgg gcgccgaccc ggacgcgctc tatcgcattt
     3001 tacgtctgac gtctgcacac gggatatttc aaacagaggg cgatcgattc aaacactcgc
     3061 cggcgtcgcg gatgctgcgc agcgatcatc cgcagtcgat gcgcagtttt gtgcggatgt
     3121 tcggcttggc taacaactgg agtgcttatg gcgagctgga acactccgtt aggactgggc
     3181 ggccagcgac ggagaagacc ctgcctgaag gattctggac ctatctcgca caggctccgg
     3241 aagcaaacag cattttcaat gagacgatgt tggccaaagc gcgcggtcag attcctgcca
     3301 tcgtcgcttc ctacgatttc tcacagtttg atgtgatcgc agacatcgcg ggcggacgcg
     3361 gccatctcat aagcgcgata ct
//
LOCUS       DASQUQ010000264         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_166578, whole genome shotgun sequence.
ACCESSION   DASQUQ010000264 DASQUQ010000000
VERSION     DASQUQ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166578"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VE486_07290"
     CDS             complement(<1..270)
                     /locus_tag="VE486_07290"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZA38931.1"
                     /translation="MRHVSQLLLLAASGSLLLLTGCGTGRVATGGAYHVTAYRPHDPS
                     KVKVKLSTSTQNLFVMEGDRLLMAVQANVGTPKDPTPRGHFTIYSK"
     gene            complement(364..1377)
                     /locus_tag="VE486_07295"
     CDS             complement(364..1377)
                     /locus_tag="VE486_07295"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZA38932.1"
                     /translation="MRSRWKRVRYQLEWIGLSLAAKLVPLLSRKSCFHLAKILGALAS
                     IIDRHGYKVALENLKVAFGDQLSEHERRKIARESFQHFARTMVDLLWSPRLTRQNLSR
                     YVEFQNFEETSRGTGPERSAIVACCHYSNFEWLSLACGLLDFKNAILAQEFKNSLLNP
                     IFTRIREQSGHEIIPRERGLVRLYKVLRRKGRIALLVDLTVRPRQGGVAIDCLGLKKS
                     VPSAHAWLHERTGAPIIPAHCESLPDGRYRVIFHPKIENTAGMTHQQIAQACWDSFEP
                     YVRKNPAPWLWMYKHWRYQPANSTRPYPFYAHVSRRFEEILPRDQGNSVEGTSRAESA
                     SFE"
     gene            complement(1450..2274)
                     /locus_tag="VE486_07300"
     CDS             complement(1450..2274)
                     /locus_tag="VE486_07300"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZA38933.1"
                     /translation="MTVETLRQVPLFESLDDATARELCGLIETLDCPAHRVLFRAGDV
                     GDAMYLIESGKVKICVHAKDGHEVTLAMLGRGDFFGEMALLDGERRSADAVVAEDARL
                     ALLSREHFLSFMRSSPDVALEMLTALANRLRQTDELLRHSATRNVNVEAAAQLTLADR
                     AADIIAEFGGSWKFILAAVLFFNLWVLINVWLLRDSPFDAYPFLLLSTGINMLAVLQA
                     PIILMSQNRQSHKDRLRAEIDYQVNLKNELALNEIIQRLKALERDYLRMTLEKQSE"
BASE COUNT          585 a          732 c          692 g          571 t
ORIGIN      
        1 tttgctgtaa attgtgaagt ggccgcgcgg ggttgggtct ttaggcgtgc ccacgttagc
       61 ttgaacggcc attagcagac ggtctccttc catcacaaac aggttttgcg tcgaagtgga
      121 gagtttcact ttcaccttgg acggatcatg gggtcggtaa gccgtcacgt gataagcgcc
      181 tccagtggcc acccgtcctg ttccgcaacc ggtaaggagc aacagtgaac cgctagcagc
      241 cagcaaaagt aattgggaaa catgtctcat agattgcata ctggaaaagc tcgacggcat
      301 tttgtcaacc gtcactaatt gcgatgttat ggcattgccg gccgcggcgg agcctctgca
      361 acctcattca aacgaggcgc tttcggccct gctcgttcct tcaaccgaat tgccttgatc
      421 ccgcggcaga atctcttcga agcgtcgcga aacgtgtgcg taaaacggat aaggccgggt
      481 ggaatttgcc ggttggtaac gccaatgttt gtacatccag agccagggag ccgggttttt
      541 tcgcacataa ggctcgaatg agtcccagca tgcttgcgcg atctgttgat gcgtcattcc
      601 ggcggtgttt tcgatcttcg ggtgaaagat cacccggtaa cgcccgtccg gcagcgactc
      661 gcagtgggcg ggaattattg gcgcgccggt gcgttcgtgc agccaggcat gcgccgaagg
      721 cacgcttttt tttaaaccaa gacaatcgat tgcgactcca ccctgtctcg gccgcacggt
      781 cagatcgacc agcaacgcaa tgcggccttt tcgccgcagc accttgtaaa gccgaaccaa
      841 gccccgctcc cgcggaataa tttcgtgtcc ggattgctcg cgaattctcg tgaaaatcgg
      901 attgagcagc gagttcttaa attcctgagc aaggattgcg ttcttaaaat ccagaagacc
      961 gcaggccagg cttagccatt caaaattgct gtagtgacag cacgcaacta tcgcgctgcg
     1021 ctcgggtccg gtgccccgcg aggtctcctc gaaattctga aactctacgt aacgagagag
     1081 attttgccgc gtcaggcgcg ggctccacaa aaggtcgacc atggtccgcg cgaaatgctg
     1141 aaatgattcc cgggcaatct tgcgccgctc gtgctccgac aattgatccc caaacgcaac
     1201 tttgagattc tccaaggcta ctttgtatcc atgccggtca atgatggacg ccagcgctcc
     1261 tagtattttc gccaaatgga agcagctttt ccgggatagc agcgggacta gctttgcagc
     1321 aagagaaagc ccgatccact caagctgata tcggactcgt ttccatcgcg aacgcatttc
     1381 ccactgtaaa cacggctatg agccgaaggc tagtgggcgg gaaatgacga atagatttcg
     1441 cgtgcttcgt tattccgatt gtttctccaa tgtcatcctc agatagtcgc gctccagagc
     1501 ttttaggcgt tgaataattt catttaacgc caattcgttc ttcaagttta cctggtaatc
     1561 gatttcggcg cgaagtcggt ccttgtgaga ctgccggttc tgcgacatca aaatgatggg
     1621 cgcttgcagg acagcgagca tgttgattcc ggtgctgagc agtaagaacg gataagcatc
     1681 gaagggcgag tcgcggagca gccagacgtt tatcagcacc cacaaattaa aaaagagcac
     1741 cgctgccagg atgaatttcc agcttccgcc gaactcggcg ataatatccg cggcccggtc
     1801 cgcgagtgta agctgcgctg ccgcctccac attgacattg cgcgtggcgc tgtggcgcaa
     1861 aagctcatcg gtctgccgaa gccggttggc gagcgcggtg agcatttcca gcgcgacatc
     1921 gggactgctg cgcataaaag acagaaaatg ctcgcgcgag agcagagcca gcctcgcgtc
     1981 ctcagccacc accgcgtcgg ccgagcggcg ctcgccatcg agcaaagcca tttcgccaaa
     2041 gaaatcaccg cggcccagca tcgccagcgt cacctcatgc ccatccttgg cgtggacgca
     2101 aatcttcact tttccactct caatcagata catcgcgtca ccgacgtcac cggcgcggaa
     2161 caagacccgg tgcgctgggc aatcgagcgt ttcgatcaat ccgcataact ccctggcagt
     2221 cgcatcatcg agtgattcaa acagcgggac ctggcgcaga gtttcaacag tcatcgtgga
     2281 aaaccgtgat caatcatagc gacaatcaac ggtggcgaga agggccaatt atctcggatt
     2341 cgacgttaat tttcgcatag ggttttgtca cccgatctgt tactcctgcc gtccattgtc
     2401 gcaaatcacc gttctgggca gcgcacgtgc ccgtttgcga cgccctcgtc ggcaaacgct
     2461 ctccaagcaa aacagctctc agaggtttgg aacgctctcg gcaattggcg agagcgccaa
     2521 tcgcaacacg caagcgcgcg tgcggtcctc tgactctgcg cgatcaacac gctttgtgag
//
LOCUS       DASQUQ010000265         4980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_800887, whole genome shotgun sequence.
ACCESSION   DASQUQ010000265 DASQUQ010000000
VERSION     DASQUQ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800887"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VE486_07305"
     CDS             <1..1312
                     /locus_tag="VE486_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38934.1"
                     /translation="SDLSRLHTELSKAEEAGDKAAIIELSRRIVAIAPNESSAWETLF
                     QTQFESEDFDSAVKSLDAWQKAAKRPPAAIEDFRGDVFAQRKDYQNAERHWLAFLAKK
                     PSAADAANTYDKLADSCADQGRWADNVAYRSKAIAAKDTPARRVFRACTLLRLHEWDA
                     AYADMAKANRMDSTDAQVKEWLPQFERLESFLPEIKALDAQIAKSPDDAGLLLERARL
                     FTLAGRPLLALSDCEHAMKIEPASMRARIQTAEALLDTARADDAAKLQVSKSLARADD
                     RHVSEQALRELGDEDASIRENPGKAEPLVARAKTLRELRQFVLALADARAALSIDDKS
                     AAAHLEAAHDLHGLDQSKEALTHAVKATELNPNDGAAWHYRGVLEAGRADFPAAIESQ
                     TRSLKLGESLEALRARENCERRIGQVGKADADLKRIRELEPAKE"
     gene            1309..>4980
                     /locus_tag="VE486_07310"
     CDS             1309..>4980
                     /locus_tag="VE486_07310"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HZA38935.1"
                     /translation="MKHAIVILFSACIGLTTLHSAPDEETSNAKFATANPSGGGPDAG
                     GVLIEPSEGQIAPGTELTITFPSAMVSADKIDLSEQPSPFVSKPKIEGNFLWKSQTEG
                     VFVVKSVVAGAKHRLTLARDLKDVSGKSVVAPGWSAEFTATPFTISSDFSEREQLNRQ
                     PQIPLETSYNVRLTEVAGHVYFQDRDSRQRFPVDVILRSDEKPTEELEAEDFRVEPRQ
                     PLPVGRTYDLIVNGLLDAKSRRPLPYLKVIPVGKTEPLKVEWIAAFNHALDEPVIRIK
                     FNDDIDPAEATPDRIRIEPAVKEMRLLASRDEVEVHGQFDLTQRYRVTVSPELKGERG
                     YGLATESRWGATFHPKESTIVFPASQIFLRARPELRFSFFQVNTPPVTWKLARIPLEK
                     LPAVTARVREFDKNATDPITGEDVVDPRTGFKKQFQTELLVQAFNLPVVTSGTVEAAT
                     GDTETRRDVRCAGPNGEPLVGIYLFEASAQLGDGRIVGNRSIVCSSDFILTQKRTPAT
                     ALIRVTKMSDAQPVGGVTIRAVTNENIELARGGTDANGLATFSLKKIFPHKKLGAHLF
                     IADTKSGPALQFVEGTTYSSGYPGGPGGKIPHAEIITDRNLYRPGQVVKMKGIVRNFT
                     GGGLTIPSGGEAHWRVLGSDGARVVGEGSATVSPDGAWEAEWTVPEKSKLGHFGISCT
                     IDNRQYDGVASIDVEEYRVPLFSVVVEAEAEVGQTAHARVSSAYFHGAPNVGARVHWK
                     ATWTAVADSGSEADGYKRRYNAYPEVGPSLDPYSEETKTIEGDAKLDARGFADLVCES
                     PFKENPAIGRANVSWRAEITSLDGQTIVGGEMASIFSNSTRLGIRATERPGKDGGVNV
                     NLDAVDKEDAKIAGVPVRVDLFHVTTKTTREQIAPFVFRYRNTDQFAKVASQETKTPA
                     EFLFPADKTGRYVVTASAPDRKTALVGDEATFTGEEPAELPVQSETSFKIEHRPEAFT
                     PGEKAVLTIQAPFGGMAWVSVETDAILETLLVPLSGNAGRIELPIKKDYAPNATVSIY
                     LVRPGGDKELPQERFAVSEIEVRRPDRELTIEPHLAKTSVKPGESIHGELRITSEGKP
                     VGDVDLVVFAVDDAVLKLGDWKLPNLLAGFYPRNPFAVRSYQSLQNYIQEITQKSLTQ
                     KGFVIGDGGEEPLSNVTNLRKEFRTLAFWEGSLKTGPDGKATFDFTAPDNLTTYRVVA
                     IGQTKANQFGGDASATVQVS"
BASE COUNT         1142 a         1367 c         1521 g          950 t
ORIGIN      
        1 cagcgatctt tcgcggctgc acacggagct ttcgaaggcg gaggaagctg gcgacaaagc
       61 cgcgattatc gagctgagcc ggcgaatcgt cgcgatcgct ccgaatgagt ccagcgcctg
      121 ggagacgctg ttccagacac aatttgaatc cgaggacttc gacagcgccg tgaagtcact
      181 ggacgcatgg caaaaagccg cgaaacggcc acccgccgcg attgaagatt ttcgcgggga
      241 tgtttttgcc cagcgcaagg attaccaaaa tgccgagcgg cattggctcg catttctagc
      301 gaagaaacct tcggcggcag acgcggcgaa cacctacgat aaacttgccg attcgtgcgc
      361 ggatcagggg cgctgggccg acaatgtggc ctatcggagc aaggccatcg cggccaagga
      421 tactccggca cgccgcgttt ttcgtgcatg cacactctta cgcctgcacg aatgggacgc
      481 cgcttacgcc gacatggcaa aggcgaacag gatggacagc accgatgcgc aagtgaagga
      541 atggttgccg cagttcgagc ggcttgaaag ctttttgccc gaaatcaagg cgctggatgc
      601 gcagattgcg aaatctccgg atgacgccgg acttctgctc gaacgcgcgc gattgttcac
      661 gctggccggg cgaccgctgc tggccctgag tgattgcgag cacgcgatga agatcgagcc
      721 ggcatcgatg cgcgctcgca tccagacagc cgaggcactt ctggacaccg cccgcgccga
      781 tgatgcggca aagttgcagg tcagcaaaag tcttgcccgt gctgatgaca gacatgtgag
      841 cgagcaggcg ctgcgcgagc tcggagatga agatgcttcg atcagggaga accccggcaa
      901 agcggaaccg ctggttgcgc gagcaaagac gttgcgagag ctgcgccaat tcgtgctggc
      961 gctggccgat gcgcgggctg cgctgagcat cgatgacaag tcggccgcgg cgcatctcga
     1021 ggccgcacac gacttgcatg gtttggacca atcgaaagaa gcgctgaccc atgcggtcaa
     1081 ggcaacggaa ttgaatccga acgatggagc ggcatggcat taccggggcg tgctcgaagc
     1141 agggcgcgcc gattttccag cggcgatcga atcgcaaaca cgctcgctga aactcggcga
     1201 atcgctggaa gcactgcgcg cccgggaaaa ttgcgaacgg cgtatcggcc aggttggcaa
     1261 ggcggacgca gatcttaagc ggatccgcga attggagccg gcgaaagaat gaaacacgcg
     1321 atcgtcattc tcttttccgc gtgcattggc ctgacaaccc tccattctgc gccggatgag
     1381 gaaacgtcga acgccaaatt cgccacggcg aatccgtccg gtggcggacc ggacgcgggt
     1441 ggcgtactga tcgagccgag cgaaggccaa atcgcgcctg gcacagaact tacgattaca
     1501 ttcccgagcg cgatggtgag cgcagacaag atagacttga gcgagcagcc atctccgttc
     1561 gtaagcaaac cgaaaatcga aggcaatttt ttgtggaaga gccagaccga aggcgtattt
     1621 gtcgtgaaaa gcgtcgtcgc gggagcaaag catcggctca cgctcgcgcg cgatttgaaa
     1681 gacgttagcg gcaaatcggt tgttgcgccc ggctggagcg cagagttcac tgcgacgccg
     1741 tttacgattt cgagcgattt ttccgagcgc gaacagctta accggcaacc gcaaatcccg
     1801 ctcgaaacca gctacaacgt gcggctaacc gaggtcgccg ggcacgttta ttttcaagat
     1861 cgcgattcgc gccagcgttt tccagtcgac gtgattctgc gctccgatga gaagccaacg
     1921 gaagagctgg aagcggaaga ttttcgcgtc gaaccgcgcc agccactgcc ggtgggccgc
     1981 acctacgatc tcatcgtcaa tggtttgctc gacgccaaaa gccgacggcc tttgccatat
     2041 ttgaaggtta ttccggttgg caagaccgag ccgctcaagg tcgaatggat cgcagcgttt
     2101 aaccatgcgc tcgatgagcc ggttatacga atcaaattca acgacgacat cgatccggca
     2161 gaagccaccc cggaccgaat ccgcattgag ccggcagtca aagagatgcg gcttcttgcc
     2221 agccgggatg aggtcgaggt gcatggacaa ttcgatctca cccaacgtta tcgcgtgacg
     2281 gtctcacccg aattgaaagg cgagcgtggc tacggccttg ccaccgagtc gcgttggggc
     2341 gcaacgtttc atccaaaaga atcaactatt gtattcccgg cttcgcagat ttttttgcgt
     2401 gcacggccgg agctgcggtt ctcgtttttt caggtcaata ccccgccggt gacatggaag
     2461 ctcgcgcgca ttccgctgga gaaattgccg gctgtgactg cgagagtgcg cgagtttgat
     2521 aagaatgcga ccgatccgat cactggagaa gacgttgtcg atccacggac tggtttcaag
     2581 aagcagttcc aaacggaatt acttgtccaa gcgttcaatt tgccggttgt aaccagtggg
     2641 acagtcgagg ccgcaaccgg cgataccgag acgcgccgcg atgtgcgttg cgccggtcca
     2701 aacggcgagc cgttggtcgg catttatctg ttcgaagcga gcgcgcaact cggtgatggc
     2761 cgaatcgtcg gcaaccgctc aatcgtttgc agcagcgatt tcattcttac tcaaaagcgg
     2821 acgccggcaa ccgccctgat tcgcgtcacg aaaatgtccg atgcgcaacc ggtcggtggc
     2881 gtcaccattc gcgccgtcac gaacgagaac atcgagctcg cccgcggggg gaccgacgcg
     2941 aacgggctgg cgacgttttc gctgaagaaa atttttccgc acaagaaact cggcgcgcat
     3001 ctttttattg ccgacacaaa aagcggaccg gcgttgcaat tcgtcgaggg aacaacctat
     3061 tcgtccggtt accctggcgg accgggcgga aaaataccgc acgccgaaat tattaccgat
     3121 cgcaatctct atcgtcccgg ccaggtggtg aagatgaagg gaatcgtgcg caacttcacc
     3181 ggcggaggcc tgacgattcc gagcgggggt gaagcgcatt ggcgtgttct cgggagtgat
     3241 ggcgcccgcg tcgttggcga aggcagcgcg accgtttcac cggacggcgc ttgggaagcg
     3301 gaatggacgg tgccggagaa atcaaaactg gggcacttcg gtatcagctg cacgatagac
     3361 aatcgtcaat atgacggtgt ggcctccatt gacgtcgaag aatatcgtgt gccgcttttt
     3421 tcggtggtcg tggaagcgga agccgaagtt gggcaaacgg cgcatgcccg cgtttcttct
     3481 gcttattttc acggagcgcc gaacgtgggc gcgcgcgtgc attggaaagc aacgtggact
     3541 gcagtagccg actctgggag cgaggccgat ggttacaagc gacggtataa cgcctacccg
     3601 gaagtaggtc cgagtcttga cccctacagc gaagagacca agacgattga aggcgacgcg
     3661 aaactcgatg cacgcgggtt cgcagatctc gtgtgcgaat cgccattcaa agagaacccg
     3721 gccattggtc gcgcgaatgt ttcctggcgc gctgaaatca catcgctcga tggtcagacg
     3781 atcgtcggcg gcgagatggc ttcgattttt tcgaattcga cgcgtttagg cataagggcc
     3841 accgagcggc cgggcaagga cggcggcgtc aatgtcaacc tcgatgctgt cgataaagag
     3901 gatgcaaaaa tcgcgggcgt cccggttcgt gtcgatcttt tccatgtgac aacgaagacc
     3961 acgagagagc agatcgcgcc gttcgttttt cgttatcgaa acaccgatca atttgcgaag
     4021 gtcgcatcgc aagagacgaa aacgccggcg gaatttcttt ttccagctga caagaccggg
     4081 cgttatgtcg tgacggccag cgcgccggac aggaaaacag ccctcgtcgg cgacgaagcc
     4141 actttcacag gcgaagagcc ggcggaactt ccggtccaga gcgagaccag tttcaagatc
     4201 gaacatcgtc ccgaagcgtt caccccgggc gagaaggcgg tcttaacgat ccaggcgcct
     4261 tttggcggca tggcgtgggt ctcggtcgaa accgatgcaa ttctcgagac gcttctcgtt
     4321 ccactgagcg gtaatgccgg ccgaattgag ctgccgatca aaaaggacta tgcgccgaat
     4381 gcaaccgtct cgatctacct cgtcaggccg ggtggcgaca aggaattgcc gcaggaacgt
     4441 tttgcggtgt ccgaaatcga agtgcgccgg ccggatcgcg aattaacgat cgagccgcat
     4501 ctggcaaaaa cttcggtgaa gccaggcgaa agcatccacg gcgaattgcg gatcacatcg
     4561 gaaggcaagc cggttggcga tgtcgatctt gtcgttttcg cagttgacga tgcagtgcta
     4621 aaacttggcg attggaaact gccaaatctg ctggccggct tttatccccg gaatccgttt
     4681 gcggtccgaa gctatcagtc gctgcagaat tacatccaag agattacgca gaagagtcta
     4741 acccaaaaag gattcgtcat cggtgatggc ggcgaagaac cgctgtcgaa cgttaccaac
     4801 ctgcgcaaag aatttcggac gctcgcgttc tgggaaggaa gcttgaagac tggccccgat
     4861 ggcaaggcga ctttcgattt caccgcgccg gataatctca cgacgtatcg ggtggtggcg
     4921 atcggccaga ccaaggcgaa tcagtttggc ggcgacgcca gcgcaacggt gcaagtttca
//
LOCUS       DASQUQ010000266         5432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1242716, whole genome shotgun sequence.
ACCESSION   DASQUQ010000266 DASQUQ010000000
VERSION     DASQUQ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242716"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VE486_07315"
     CDS             complement(<1..266)
                     /locus_tag="VE486_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38936.1"
                     /translation="MLLALQEAFRAVLSASAMRKPLLLLVTLLAGAGLIKGEIKTPED
                     TPIEITSTGETTYENGLATARDNVAIHIGDTDIYADYAQYNSST"
     gene            complement(317..1510)
                     /locus_tag="VE486_07320"
     CDS             complement(317..1510)
                     /locus_tag="VE486_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZA38937.1"
                     /translation="MSLAIAGMGWVTPLGNGVDPVWEQLLQGYEATPAKISEEFGDRT
                     YSAFRVPESVLSSLAPHPRLRRASVISRFAAAAGLEALQAAGVKLDSQTAERTALVFA
                     ISNGGVIYTKRFYRDIVEAGAQSASPLLFPETVFNAPASHLAAILGVTGTTYTLVGDS
                     AVGLLAISMANDLMANDALDYCLVVGAEEADWLLCDAYRRWRLLRLTPPVEPFTEKKR
                     GMILSEGAGAVLLARLPSRSAAEAGDGPVVIERVHPGMSYRNRAEAEEILKEILCDLS
                     EMDIDFVISSANGTFIDQAECEALRRTLPDAFLYTAKPALGESVGAAGLWQVIVGTQA
                     LRTGELPPLLHAGPMISLRTSVSRMPVPRAQHAIVLSCGVNQQAAGLRLRKSSSPLII
                     PASIQ"
     gene            complement(1507..2733)
                     /locus_tag="VE486_07325"
     CDS             complement(1507..2733)
                     /locus_tag="VE486_07325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZA38938.1"
                     /translation="MPKTNVGNGKSSTPRVAVTAAGVVSPLGFGLSETLDSLRAARDC
                     ITQVTRFSVAECRCKTAGQVPDGRLLEEQHDTRRAKRLHRASHMLIRALAEALAQRPG
                     FKPELTVIGTTSGGMSYGEDYYRSLRRNGNLRHSPTWVANYTAQKPVIDAQEAFGISA
                     PCQIIANACASGTNAIGHAYECVRSGRYQRVLTGGYDALSELVFVGFDSLQASTAEKC
                     RPFDRDRTGMALGEGAAVLALENLDVARERGAPVLAEIIGYGISTDNFHMTQPNPSGI
                     GPRQAMERALQSARLSADKVDYINAHGTATAFNDAAEGKAVSELFDGVPVSSTKSMMG
                     HSLGAAGAIEVIICLLALQHRFLPPNINFRAPDAGMDINIVANEARPMALRTVLSNSF
                     GFGGTNASIVIQKSEA"
     gene            2923..3285
                     /locus_tag="VE486_07330"
     CDS             2923..3285
                     /locus_tag="VE486_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZA38939.1"
                     /translation="MGTKLYVGNLSFNTTENELQELFSQAGAVQEVTLMQDKFTGKSR
                     GFAFVTMGSEQDAQNAISKFNGQTIEGRALTVNEARPREPRPPGGGGRGYGGGGGGGG
                     YGGGGGYGRREGGQRRGH"
     gene            complement(3338..3421)
                     /locus_tag="VE486_07335"
     tRNA            complement(3338..3421)
                     /locus_tag="VE486_07335"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3385..3387),aa:Tyr,seq:gta)
     gene            complement(3496..4722)
                     /locus_tag="VE486_07340"
     CDS             complement(3496..4722)
                     /locus_tag="VE486_07340"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZA38940.1"
                     /translation="MRVPLLDLSEQYRALAEPIREAIDDVLASRRFILGPKGEAFEKA
                     ICAYCKAPHAIGVSSGTDALLAILMALGIGRGDAVITTPYTFLATAGCIARVGATPLF
                     VDIDPATYNISPAALETFLEKECEAVGEDVVFPRAKQGKLTASPTRDGSLRIRAIVPV
                     HLFGLCCEMEPIHQISEDYQLDVIEDAAQAIGAEYPFGGKTARAGAMGEAGFFSFYPS
                     KNLGAAGDAGMILCRDDQLARQMRIFRDQGMDPRYYHGVIGGNFRLDEIQAAILAVKL
                     PHLESWSVARRAAADFYGTEFARLGLTEKIVLPAESYRERGLTNHHIYHQYVIRAPMR
                     DALREHLAEREIETAIYYPLGLHEQECFAYLGYKKGDFPETERAARETLALPIYPEIS
                     RDAQRCVVGAIAEFLE"
     gene            4862..5038
                     /locus_tag="VE486_07345"
     CDS             4862..5038
                     /locus_tag="VE486_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38941.1"
                     /translation="MALLRGLFWLVLFVVLAFSFVVLFEYGPHHFASGFQKEFARAKT
                     FVVKQTEKISKPKK"
     gene            complement(5190..>5432)
                     /locus_tag="VE486_07350"
     CDS             complement(5190..>5432)
                     /locus_tag="VE486_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HZA38942.1"
                     /translation="LEIIRESIEFGLAHRNEAVRYSLPYARDMNADLASKFIGMYVNE
                     FTRDYGEIGRTAIRKFLGAARDKGYIDGPIDVEFIE"
BASE COUNT         1196 a         1528 c         1503 g         1205 t
ORIGIN      
        1 tgcgtgctgg aattgtactg tgcgtaatcg gcataaatat ctgtgtcgcc gatatgaatg
       61 gcgacattat cccgcgccgt tgccaggccg ttttcatatg tggtttctcc ggtggaggtg
      121 atttcaattg gagtatcctc cggagtcttg atctctccct taatcaaacc ggcgcctgcg
      181 aggagagtga cgagcagcaa gagcggcttt cgcatggcgg acgccgaaag tactgcacgg
      241 aaagcctcct gcaaagccaa aagcattctg cgctgattac gggagcgcag gcaaatgcgt
      301 tggattactg tgccaatcat tggatcgacg ctggaataat caacggtgac gaactctttc
      361 tgagtcgcaa acctgctgct tgctgattga ctccgcagct taaaacaatt gcgtgctgcg
      421 cgcgcggcac gggcatgcgt gagaccgaag tccgtagcga aatcatcggc cccgcatgaa
      481 gtaagggcgg aagttcgcca gtgcgcaaag cctgcgttcc tacgatgacc tgccacagtc
      541 ccgcggcgcc aacgctttcg cccagcgctg gtttggccgt gtaaaggaat gcgtccggga
      601 gagtgcgcct tagcgcctcg cattctgcct ggtcgatgaa cgtgccgttt gcgctcgaaa
      661 tgacaaaatc gatatccatt tcgctgagat cgcacagaat ttccttcaat atttcctctg
      721 cttctgcccg gttccggtaa gacataccag gatgaacgcg ttcaattacg accgggccat
      781 ctcccgcctc cgctgcgcta cggctcggca ggcgtgccag caggaccgct cctgctcctt
      841 cactcaaaat cattccccgc tttttctcag tgaatggctc taccggaggc gtcaatcgaa
      901 gcagacgcca tcgccgatat gcgtcgcaca acaaccaatc ggcttcttca gctccgacga
      961 ccaggcaata atcaagcgcg tcatttgcca tcagatcgtt cgccatgctt atcgccagga
     1021 gacccaccgc actatcgccg accagcgtat aagtggtgcc ggtaacgccg agaatcgctg
     1081 caagatggct cgccggggca ttgaagaccg tctccggaaa aagaagcgga ctggctgatt
     1141 gcgcgcccgc ttcaacgatg tcgcggtaaa agcgcttggt gtaaataacc ccgccatttg
     1201 agatcgcgaa aacgagagcg gttcgttccg cagtctgcga gtcgagcttc actccggccg
     1261 cttgcaatgc ctccagaccc gcggctgcgg cgaagcgcga aatcacgctg gcacggcgca
     1321 aacgcgggtg aggcgccagg ctgctgagca cggattcagg tactcggaag gcgctgtaag
     1381 ttcgatcgcc gaactcttcc gaaatttttg ctggggtcgc ttcgtatcct tgaagcaact
     1441 gctcccatac cggatcaacg ccgtttccga gaggtgttac ccaccccatg ccggcgatgg
     1501 ccagactcat gcctcggact tttggatcac aatcgacgca ttcgtcccac caaatccgaa
     1561 cgagttcgag aggacggttc gcagagccat gggacgcgcc tcgtttgcaa cgatgtttat
     1621 gtccatgccg gcatctggag cgcgaaaatt gatgttggga ggaagaaatc ggtgctggag
     1681 cgccaggagg cagatgatta cctcgatcgc tcccgccgcg cctaatgaat ggcccatcat
     1741 gctcttggtc gagctgaccg gtacgccatc aaataattcg ctgactgctt tgccttccgc
     1801 tgcgtcgtta aaagccgtgg ctgttccgtg cgcattaatg taatcgacct tatccgctga
     1861 aaggcgggcg ctctgaagtg cgcgttccat cgcctgtcgc ggtccgatac cggagggatt
     1921 cggttgcgtc atgtgaaaat tatccgtcga aatgccgtag ccaattattt cggcgagaac
     1981 gggcgcgccc cgttcccggg caacatcgag attttccaac gccagaacgg ctgcgccttc
     2041 tccgagggcc atgccagtac gatctcgatc gaatgggcgg catttttctg ctgtcgaggc
     2101 ctgaagtgaa tcaaaaccga cgaagaccag ttccgaaagc gcgtcatatc cgccggttaa
     2161 cacgcgctgg taacggcccg agcgcacgca ctcatacgca tgaccgatcg cgttcgtgcc
     2221 ggaggcgcag gcattcgcaa tgatctgaca gggagcagaa attccgaatg cttcctgcgc
     2281 gtcgatcacc ggcttttgcg cggtgtaatt tgcaacccag gtcggcgagt gccgcaaatt
     2341 cccatttcga cgcagcgaac ggtaataatc ttcgccgtaa gacatgcccc cgctggttgt
     2401 tccaatgacg gtcaattcag gtttgaaccc cggtctttgg gccagcgctt ctgctaacgc
     2461 gcgaatcagc atgtgcgacg cacgatgtaa gcgttttgcg cgacgcgtat cgtgttgctc
     2521 ttcgagcagg cgcccgtccg gtacctggcc tgcagtcttg catcgacatt cggccaccga
     2581 aaaccgggta acttgcgtga tgcagtctcg ggctgctcgc agagaatcga gggtctcgct
     2641 caatccaaac ccgagcgggc tcacgacacc cgctgccgtc accgcgacac gtggagtgct
     2701 gctttttcca tttccaacat tggttttggg catttttttg gcaaagaagt tagcctgcga
     2761 tttttattgc tttcttaatc gaagcatttt actagtgggc cgaaacggaa acggaaacgg
     2821 aagcttggaa gttggtggat tgtcagttga gtttattcag tggcgatttg aaatccgata
     2881 agcgggaaca gtgacctggc tgcgttttcc agaataaact caatgggaac taaattatac
     2941 gtaggcaatc tttccttcaa cacgacggaa aacgaactgc aggaactttt ttcacaggct
     3001 ggcgccgtgc aggaagttac tctcatgcaa gataaattca ccggcaagtc gcgtggcttt
     3061 gcttttgtca cgatgggctc cgagcaggat gctcaaaatg cgatttccaa attcaatggc
     3121 caaaccattg aagggcgggc actgaccgtg aatgaggccc gtccgcggga gccgcgacca
     3181 cccggcggtg gcggacgcgg ttacggcggc ggtggtggtg gcggaggtta tggcggaggc
     3241 ggaggctacg gccgccgcga gggtggccag cgaagaggcc attaattccg aagacattcg
     3301 caagtaaggt ccgccggaag gccgtcgagg gtgaaattgg gcagggctgg attcgaacca
     3361 gcgaaggcgt aaagccagca gatttacagt ctgccccgtt tggccacttc ggtacctacc
     3421 cggcggtgcg agcgttgata taggctcagg gccgacgcgc tgcaagtgat ttgggcgcag
     3481 ccgcagcaga gttgtctatt ccaagaactc cgcgatcgcg ccgacgacac atcgctgcgc
     3541 gtcacgcgaa atctccggat aaatgggcag ggcgagcgtc tcgcgcgcag cgcgttcggt
     3601 ttcgggaaaa tcgcccttct tgtaaccgag ataggcaaaa cattcctgct catgcaatcc
     3661 cagcgggtaa tagatcgccg tctcgatctc gcgctcggcg agatgctcac gcaaggcatc
     3721 ccgcattggt gcgcgaataa cgtattgatg ataaatgtgg tggttcgtta gcccacgctc
     3781 gcgataagat tcggctggca ggacaatctt ttccgtcaac ccgaggcgag cgaattccgt
     3841 accgtaaaaa tccgcggcag cacgtcgtgc caccgaccaa ctttccaagt gcggcagctt
     3901 aacggcgaga attgccgcct ggatttcgtc caggcggaaa ttgccgccga ttacgccgtg
     3961 ataataccgc gggtccattc cttgatcgcg gaaaatgcgc atttgtcttg cgagctggtc
     4021 gtcccggcac aggatcattc cggcatcacc agcggccccg aggttttttg atggataaaa
     4081 gctgaaaaac cccgcttcac ccatcgctcc tgctcttgct gttttcccgc caaacggata
     4141 ttccgcgccg attgcctgcg cggcatcttc gatcacatca agctggtaat cctcggaaat
     4201 ttgatggatc ggctccatct cgcagcacaa accaaacaaa tggactggca caattgcgcg
     4261 gatgcgcagg gatccgtccc gtgtagggga agctgtcagc ttcccctgtt tcgctcgtgg
     4321 gaagacaacg tcttccccta cagcctcaca ttctttctca agaaatgttt caagagctgc
     4381 cggtgaaata ttgtaagtcg ccggatcgat atctacaaaa agcggcgtcg caccaacacg
     4441 cgcgatgcag ccagccgtgg caagaaaagt gtaaggcgtg gtgatgacgg catcgccacg
     4501 cccaatccca agtgccatta aaatggccag gagagcatcc gtcccggatg atacaccgat
     4561 agcatgagga gccttgcaat aggcgcaaat cgctttctcg aaagcctcac ctttcggccc
     4621 gaggatgaac cggcgactcg ccagaacgtc gtcgatcgct tcgcggatcg gctctgcgag
     4681 ggcgcgatat tgttcgctaa gatcgagcaa cggcacgcgc atggcgccaa aagcatggca
     4741 gtttcagtcg tccgttcaag gaagatagac ttccagtctg tcccggccga caagcatctt
     4801 gcttgtcaga tagccttccg caacgttgac cctcacctcg ggctgattac tctgtctgat
     4861 aatggcactt ctgcgtggtc tcttctggct cgttcttttc gttgttctcg ctttttcctt
     4921 tgtcgttctg ttcgagtacg ggccgcatca cttcgcgagc ggttttcaaa aagaatttgc
     4981 ccgcgcaaag acgttcgtcg tgaagcagac agaaaaaatc tcgaaaccga agaaatagcc
     5041 gctgacagtc gcctcaaatt gtagtcttta ccgcggcgcg aaagaagagc agtttcttct
     5101 ccctggggga gaaggttggg atgagggctc cttgcgagtt gctgcttccg ctgcgaccgc
     5161 aaattcccac ctgtgcaagc cgtgttgtat cattcgataa attccacgtc gatcggacca
     5221 tcgatatagc ctttgtcgcg cgcggcgccc aggaacttgc gaatggccgt gcgtccgatc
     5281 tcaccgtaat cgcgagtaaa ttcgttcaca tacatcccaa taaatttgct ggcgaggtcc
     5341 gcattcatat cacgcgcata aggaagagaa tagcgcaccg cttcattccg atgcgccagg
     5401 ccgaactcga tgctttcccg gataatttcg ag
//
LOCUS       DASQUQ010000267         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_804955, whole genome shotgun sequence.
ACCESSION   DASQUQ010000267 DASQUQ010000000
VERSION     DASQUQ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804955"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VE486_07355"
     CDS             <1..386
                     /locus_tag="VE486_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38943.1"
                     /translation="PEDGALVLELMHFAEELADPSKLHVPKKVAVGKREINMATALID
                     SMTSKWKPQKYRDDYREALMEVIEEKVEAGGKEIEEKPKKAPKPTKVIDLVSVLQKSL
                     EQTGAKRKTTAKSRRKQKQPAKKAA"
     gene            549..980
                     /locus_tag="VE486_07360"
     CDS             549..980
                     /locus_tag="VE486_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZA38944.1"
                     /translation="MNALNRFADPVYCIMRLIVGLVFACHGGQKLLGFPGGGHGAEGL
                     MLVGGIIELVGGLMIAFGLLTRLAAFVSAGEMAVAYFMVHAAGKAIGHPPTPTEQFFP
                     ILNKGELAVVLCWVFLFMMFYGPGHWSIDALFKKKSTATVT"
     gene            1062..1856
                     /locus_tag="VE486_07365"
     CDS             1062..1856
                     /locus_tag="VE486_07365"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZA38945.1"
                     /translation="MKRILFALVFILIAPFIHAQEVKDFGKERLLKSPRHGEWVDFKS
                     GDRTVKAFIVYPERKEKAPVVLVVHEIFGLTDWVRSLCDELAEAGVIAIAPDLLSGQK
                     FEDVDGARKATYALPKEQVKADLDAASDYALAKIPSSNGTLAVCGFCWGGGVTFSYAN
                     ENPKLKAAYSFYGTAPDSAEGVKNIACPVYGFYAENDQRVNATIPKAEELMKAAGKKY
                     EPVIYKGAGHGFMRSGEPQNPEAREGDKKARDDAWVRWKKLLQQLP"
     gene            1970..>3355
                     /locus_tag="VE486_07370"
     CDS             1970..>3355
                     /locus_tag="VE486_07370"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZA38946.1"
                     /translation="MKILKFGGSSLATTDCIRGVARIVLEEARRGPIIVVLSAFQGVT
                     NQLLDCARLAEKNDRRYDAAWKKIAGRHRSAVESLVGRRRGIRIRSQVDTLLGDLHDA
                     LHGVQLLGHSPPRALDLIASFGERLCALIVAAHLARVHPARFVDARQLVVTDNQFTNA
                     TVIFSKTNRATRRYFTDLFQLRRRRPIPVVTGFIGSTSDGQTTTIGRNGSDYTAAILG
                     AALGASVIEIWTDVDGVLSADPKAVALAFVLPRITYEEAMELSYFGAKVLHPAAIAPA
                     VARRIPLLIKNTFHPEAPGTLISGDRSDGDQLVKGISSIAGLTLLTIRGLSMVGVPGI
                     AERLFRALASRGVNVILISQASSEHTICFAIREADTALAIAAVRHEFRFEFQNHLTSL
                     DEKPNQAIVAVVGDEMRGHPGVSGKVFGALGRHNINISAIAQGASERNISCVIDVAQQ
                     ARALNIIHQAFF"
BASE COUNT          746 a          897 c          965 g          747 t
ORIGIN      
        1 aaccggaaga cggcgcgctc gttctcgagc tgatgcactt cgccgaggaa cttgccgatc
       61 cgagcaagct ccacgtcccg aaaaaggtgg ccgtgggaaa gcgcgagata aacatggcga
      121 cggcgctcat cgacagcatg acctcgaagt ggaagccgca aaaatatcgc gacgattatc
      181 gcgaagcgct catggaagtg atcgaagaga aagtggaggc tggcggaaaa gaaattgaag
      241 aaaaacccaa gaaagcgccc aagccgacca aagtcatcga tctggtttcc gttttgcaaa
      301 agagcttgga gcaaaccggc gcgaagagga aaacgaccgc caagtcccgc aggaaacaga
      361 aacaacctgc caagaaggcg gcgtgaggcg aatgacgaat accgaatgtc taatgacgaa
      421 ccggagtgat gaaacgtgtt ctccacgtca attcgtcatt tgagcattgg gacttccttt
      481 gtcattcgtc attcgtgctt cgtcattttc ttgacatgtc agattcacat cgttcagcct
      541 gaacggctat gaacgctctc aatcgatttg cagatccggt ttattgcatc atgcgactga
      601 tagttggtct tgtgttcgct tgtcacggtg gacaaaaact tctcggattc cctggcggcg
      661 gacacggtgc ggagggtctg atgctcgtcg gcggaatcat cgaactcgtc ggcgggttaa
      721 tgattgcgtt cggcttgctc acgcgtttgg cagcctttgt ctccgctggt gaaatggccg
      781 ttgcgtactt catggtgcat gcggccggca aagcgatcgg tcatccacca acgccgactg
      841 agcaattctt tcccattctg aacaagggag aactggcggt cgttctctgc tgggtatttt
      901 tgtttatgat gttttatgga cctgggcact ggagcatcga cgctttgttt aagaagaagt
      961 cgactgcaac tgtaacttag cgcggggtct tgtcattccg ggcaaagtcg cttgcctcgc
     1021 cgtagcttta tgcgaaggct gggaggaaat ctctaactac tatgaaacga attctcttcg
     1081 cgctagtgtt tatcctgatt gcgccattca tccacgcgca ggaagtaaag gattttggga
     1141 aggagcgcct cctcaaatcg ccccgccatg gcgaatgggt cgatttcaaa tccggcgatc
     1201 gcaccgtcaa ggcgttcatc gtttatcccg agcgcaagga gaaagccccg gttgtgctcg
     1261 tcgttcacga aatctttggc ctgaccgatt gggtgcgcag tctgtgcgac gagttggctg
     1321 aagccggcgt catcgctatc gcgccggatt tgctcagtgg ccaaaagttc gaagacgttg
     1381 atggtgcgcg gaaggcgacc tatgcgttac cgaaagaaca ggtcaaagcc gatctcgatg
     1441 cagcatccga ttacgcgctg gctaagattc cgtcgtcgaa cggcacgctg gcggtgtgtg
     1501 gattttgctg gggcggggga gtgacgtttt cctacgccaa tgaaaatcca aaattgaagg
     1561 cggcctattc cttttacgga acggcgccgg attctgcaga aggagtgaaa aacatcgcgt
     1621 gcccggttta tggcttctat gccgagaacg accaacgcgt gaacgcgaca attccgaaag
     1681 cggaagagct aatgaaagcg gctgggaaga aatacgagcc cgtgatttac aagggcgccg
     1741 gccacggttt catgcgttct ggtgaacctc aaaatcccga agcgcgggaa ggcgacaaaa
     1801 aagcgcgcga cgacgcctgg gtgcgctgga aaaagttgct ccagcaactg ccatagtcgg
     1861 tggtaggatt ctccgcaggc catcgctgga aattcgtcag acgtatgccg acaaaatttg
     1921 tggcgggccg cgatcctcgt cgcgttcacc gctaccttgg ttccgaccca tgaaaatcct
     1981 taagtttggt ggttcttcgc ttgccacaac cgattgcatc cggggtgtcg ccaggatcgt
     2041 tcttgaggaa gcgcggcggg gaccgatcat cgtagtgctt tccgcttttc agggcgtgac
     2101 caaccagctt ctcgattgtg cgcggctggc ggaaaagaat gatcggcgct acgacgcggc
     2161 gtggaaaaaa atcgccggcc gccaccgctc cgcagttgaa agtctggtcg gtcgacgtcg
     2221 aggtatccga atccgatcgc aagtcgatac acttctcggc gatttgcatg acgcgcttca
     2281 cggcgtccag ctcttaggtc acagtccacc acgtgcgctc gatctaatcg cgagtttcgg
     2341 cgaacggctg tgcgcgctaa tcgtggcggc acatctggcg cgggttcatc ccgcccggtt
     2401 tgttgacgcg cgacaactcg tagtcacgga caaccagttc acgaacgcga ccgtgatctt
     2461 tagcaaaacg aatcgcgcga cccgacgata ctttacagat ctgttccaac ttcgccgtcg
     2521 tcggccgata cctgtcgtga ccggatttat tggatcgacc agcgacggcc aaactacaac
     2581 aatcggccgc aatggatccg attacacggc tgcgattcta ggcgctgctc ttggtgcctc
     2641 cgtgattgag atctggacgg atgtcgacgg cgttttgagc gccgatccga aggcggtcgc
     2701 cctcgcgttt gttttgccgc ggatcacata cgaagaagcg atggagctgt cgtatttcgg
     2761 cgcgaaggtg cttcaccccg ccgcgattgc tccggccgtc gccagacgga ttccgcttct
     2821 gatcaagaat acgttccatc cagaggcgcc gggtacgctc atttcgggtg atcgcagcga
     2881 tggcgatcaa cttgtgaagg ggatcagctc aatcgcggga ctgacgctgc tcaccattcg
     2941 gggcctgagc atggtcggag ttcctggaat cgccgagcga ttatttcgtg cgctggcatc
     3001 tcgcggcgtc aacgtcatcc tcatctccca agcctcatcc gagcacacga tttgtttcgc
     3061 catccgcgag gccgataccg ctttagcgat tgcggccgtg cgtcacgaat ttcggttcga
     3121 attccagaac catttgacat cgctcgatga aaaaccaaat caagcgatcg tcgcggtcgt
     3181 cggcgatgag atgagaggac accccggtgt ctccggcaaa gtgttcggag cgcttggccg
     3241 gcataacatt aatatcagcg cgattgccca gggcgcgtca gagcgcaata tctcttgcgt
     3301 catcgacgtg gcgcagcaag cgcgtgcgct gaatatcatc catcaagcgt tcttc
//
LOCUS       DASQUQ010000268         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_442118, whole genome shotgun sequence.
ACCESSION   DASQUQ010000268 DASQUQ010000000
VERSION     DASQUQ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442118"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VE486_07375"
     CDS             complement(<1..927)
                     /locus_tag="VE486_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZA38947.1"
                     /translation="MKSPLEAPEKQIAEETSAKPEKPVPGVIDTSKMAKGQREALELA
                     EAARDPLEDRGSFASNLFVGRYDFDRIYPYLAQSPEDRAAGEDFLHKLESYLRDHVDP
                     DEIDRTGEIPPENFKGLAEIGAFGIKVPKQYGGLGLSQCNYGRAAVLLGSWDGNVAAL
                     VSAHQSIGVAQPLLLFGTEEQKQKYLPRVAGGEISAFALTERHAGSDPATMSLRAEPT
                     PDGSEFILNGEKLWCTNGVKAGVLVVMARTPSKMVGGKERKQITAFIVDVDTPGLEIT
                     YRCRFMGLRALYNAVVKFTDVRVPRENIISKEG"
     gene            complement(924..1061)
                     /locus_tag="VE486_07380"
     CDS             complement(924..1061)
                     /locus_tag="VE486_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38948.1"
                     /translation="MRRNRSTDILSVGQAGVSPADYQFKSNGTPGKMPGVPTGWKPAL
                     R"
     gene            complement(1051..3165)
                     /locus_tag="VE486_07385"
     CDS             complement(1051..3165)
                     /locus_tag="VE486_07385"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZA38949.1"
                     /translation="MPAVATGSMIRREIGDDHICLLTFDRPESGANIFDAATLDELNE
                     HLAFVENDASLRGVIIASAKKSIFIAGADLKTLLQQAQTGDMRAFIAEGQRVFNRFAE
                     LEIPTVAAIHGACAGGGYEVTLACDYRVASDDPATRIGLPETTLGLIPAWGGCTRLPR
                     LIGAERAAEVILKGKLYSAQEASKLGLVDEVAPRDQLVDRARQKLRDGKRKPSTAPKI
                     DQEIPAPRDHNPAPARALEVIKKGLFASTQQSLAFELDTIVDLGQTESTQNLIRNFFL
                     AEKYKKGSSKAAVPRVVHAAVIGAGVMGSGIAQWLSSRGVTVILRDIAREQIDRGLAN
                     IEKTYADAVKRGLMSEEKAKEGRARIVASTAPMELRDVQIVIEAASEKIEIKREIFRE
                     LAMQAGPKTIIATNTSALPLSQLADATVSPEHVIGLHFFNPVSRMKLVEVVVAKQTSD
                     DTRERTLAFARQIEKLPVLVRDSPGFLVNRVLFPYLLDAAELFESGIEADKIDNALAQ
                     WGMPMGPLRLIDEIGVDITIDIGNTLEKAYGRRDHVPAVLLWLRDREMLGRKTGAGFY
                     KYEGKEQAPNESLAQWRRGLHGDPEGAEGPVIPPEMHRDPRLTLGEDQLTHRLTFLMV
                     NEAARCVEEKIVDSPEDADYGMILGTGFAPFRGGPLRFAEHFGLRKIVDELERLGQSE
                     EKFSPCEILKKHARDGTKFYET"
     gene            3357..>4566
                     /gene="mgtE"
                     /locus_tag="VE486_07390"
     CDS             3357..>4566
                     /gene="mgtE"
                     /locus_tag="VE486_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZA38950.1"
                     /translation="MVGKILQPEIKSLIDARDFGALREVFREWPPADVAEVILDVPED
                     EQVIIFRVLPHTLAADVFEYLDFDAQQKLLRAMAHEQVVSILNEMSPDDRTALLEELP
                     SAAARQLIQLLTPEERRIAQSLLGYPEGSVGRLMTPDFIAVHEDWTVQQVLDFVREHG
                     QDSETLNVVYVVDDRGKLIDDIRMREFLLKPLTIKVSDIRDQVFVALSVSDSQQDALN
                     IFRKYDRTALPVVDSRGVLVGIVTIDDMLDVAEQEATEDIQKFGGMTALDEPYMRLSL
                     TEMVRKRAPWLIILLLGEMLTASAMAYYEEELAKALVLAVFLPLIIASGGNSGSQAST
                     LMIRAMALGEVTLRDWWSVMRREFQAGLILGLCLGTIGAIRVGAWSIIGERYFQREVY
                     GPHWALIALTV"
BASE COUNT          919 a         1332 c         1286 g         1029 t
ORIGIN      
        1 gccttccttc gagatgatgt tttcgcgagg cacgcgcacg tcggtgaatt tcaccaccgc
       61 attgtaaagc gcgcgcaatc ccatgaaccg gcagcgatac gtgatctcca gtcctggtgt
      121 atcgacatcg acaatgaacg cggtgatttg cttgcgttct ttcccgccca ccatcttcga
      181 cggcgtgcgc gccatcacca ccaagacccc cgctttcacg ccgttggtgc accaaagttt
      241 ttcgccgttt aagatgaact cgctgccgtc gggagtgggt tcggctcgga ggctcatcgt
      301 cgccggatct gaaccggcgt gtctttccgt cagcgcgaat gcggaaatct cgccgcctgc
      361 gacgcgcggc aaatactttt gtttctgctc ctctgttcca aacaagagca acggctgcgc
      421 gacaccgatt gattgatgcg cggaaacgag cgcggccacg tttccgtccc aactgcccag
      481 caaaacggcc gcgcgcccgt aattgcattg tgacaaaccg agcccgccgt attgcttcgg
      541 cactttgatc ccaaacgcgc cgatctccgc gagtcccttg aaattttccg gtggaatctc
      601 accggtgcga tcgatctcgt ccggatcgac gtgatcgcgc agatagcttt ccagtttgtg
      661 caaaaaatct tcgcccgccg cgcgatcttc agggctctgc gcgagatacg ggtaaattcg
      721 atcgaaatcg tagcggccga cgaaaagatt gctcgcgaaa ctgccgcgat cttcaagtgg
      781 atcgcgcgcg gcctcggcca gctcgagcgc ctcgcgctgg cctttcgcca tcttcgatgt
      841 gtcgatgaca cccggcacag gcttttccgg cttcgcgctg gtttcttcgg cgatctgctt
      901 ctccggtgct tccagtggcg atttcatcgt agcgcaggct tccagcctgt ggggacaccg
      961 ggcatcttgc ctggtgtccc gtttgatttg aattgataat cggcaggcga gacgcccgcc
     1021 tgccccacag acaagatgtc tgtgctacga ttacgtctca taaaatttcg ttccgtcgcg
     1081 cgcgtgcttt ttcaatatct cgcacggcga aaatttctct tcgctttgcc ccagccgttc
     1141 tagttcatcg acgatcttcc tgagtccgaa atgttccgcg aaacgcaatg gcccgccgcg
     1201 gaatggcgcg aaacctgttc ctaaaatcat tccgtaatcc gcgtcctcgg gcgaatcgac
     1261 tatcttctct tccacgcaac gcgcggcttc gttgaccata aggaaggtta atcggtgcgt
     1321 aagctgatct tcgcccaacg ttaatcgcgg atcgcgatgc atttccggcg gaatgactgg
     1381 cccttcggcg ccttccggat cgccatgcaa tccacgccgc cactgcgcga gcgactcgtt
     1441 cggtgcttgc tctttacctt cgtatttgta aaagccagcc ccggttttac gcccgagcat
     1501 ctcgcggtcg cgcaaccaaa gcaggacagc tggaacatga tcgcgccgcc cgtaggcttt
     1561 ctcgagcgta ttgccaatat cgatcgtgat atcgacgccg atttcatcga tcaatcgcag
     1621 cggccccatc ggcatgcccc attgcgcaag cgcattgtcg atcttgtccg cctcgatgcc
     1681 gctctcaaaa agctcagccg catcgagcaa ataaggaaac aagacgcggt tgacgaggaa
     1741 acccgggctg tcgcgcacaa gcacgggcag cttttctatt tgccgtgcga aggccagtgt
     1801 ccgctcgcgc gtgtcatccg aagtttgttt ggcaacgaca acttccacca gcttcattcg
     1861 actaaccgga ttaaaaaaat ggagaccgat gacgtgctcg ggcgaaaccg tggcatcggc
     1921 cagctggctg agcggaagcg cggatgtgtt cgtggcgatg attgtttttg gacccgcctg
     1981 cattgccagc tcgcgaaaga tttctctttt gatctcgatc ttttcggagg cggcctcgat
     2041 cacaatttgc acatcgcgca attccatcgg cgccgtagag gccacaatgc gggcgcgccc
     2101 ttctttcgct ttctcttcgc tcatcaagcc gcgcttcaca gcgtcggcgt aagtcttttc
     2161 gatattcgcc aggccgcggt cgatttgctc ccgcgcgata tcgcgcagga tgaccgtaac
     2221 gccacgcgag ctgagccatt gggcaatgcc cgagcccatt acgccggcgc caatcaccgc
     2281 ggcatgcacg accctgggca ctgccgcttt ggaactgcct ttcttgtatt tctcggccag
     2341 gaaaaaattg cggatcaggt tttgcgtcga ttcggtctgg ccaagatcga caatcgtgtc
     2401 cagctcaaag gcgagtgatt gctgagtcga agcgaaaaga cctttcttga ttacttccag
     2461 ggcgcgggcc ggcgccggat tatgatcacg cggcgcggga atttcctgat caatttttgg
     2521 cgccgtcgat ggtttgcgtt tcccgtcgcg caacttttgc cgtgctcgat cgactagttg
     2581 atctcgcggc gcgacttcat cgacgaggcc aagtttcgag gcttcctgcg cagaataaag
     2641 tttccctttc aaaatcactt cggcggctct ctctgcgcca atcaaacgcg gcaggcgcgt
     2701 gcaaccgccc caagcaggaa ttagcccgag ggtggtttcg ggaaggccga tccgcgtggc
     2761 cggatcatcc gaggcgaccc gataatcaca cgccagggtg acctcatagc ccccgcccgc
     2821 acaagcgcca tggattgcag caaccgtggg gatctccaat tcggcaaacc gattgaagac
     2881 ccgctgtcct tccgcgatga acgcgcgcat gtcgccggtt tgcgcttgtt gcaacagcgt
     2941 cttcaaatcc gccccggcga tgaagatcga ttttttcgcc gatgcgatga tcacgccacg
     3001 caacgaggca tcgttctcga caaaagcgag atgttcattc agctcatcga gagtggcggc
     3061 gtcgaaaatg ttcgcgcctg attcgggacg atcgaacgtg agcaggcaaa tgtgatcgtc
     3121 accgatctca cggcgaatca ttgagcctgt cgcaacggcc ggcataggaa ttaccttggc
     3181 tctttttcgc gcaaattcca agcggaacag cactgggatg atggagtagt ggagtaaggg
     3241 agtgatgtga gcaaatgaga cagatgagaa gaatggcatt gctccattac tccaacactc
     3301 cactattccc gtgctgctcc gttgctttgg cgatgcgcga gtgttagaaa cgcggcgtgg
     3361 tcggcaaaat cctacaaccg gaaataaaga gcctgatcga cgcccgtgat ttcggtgcgc
     3421 tgcgcgaggt gtttcgggaa tggccgccgg ccgatgtcgc ggaagtcatc ctcgacgtgc
     3481 cggaggacga acaggtcatc atctttcgcg tgctgccgca tacgctggcg gcggatgttt
     3541 ttgaatacct cgatttcgac gcgcagcaga aattgctgcg cgccatggcg cacgagcagg
     3601 tcgtgagcat tctgaatgaa atgtcgccgg acgatcgcac ggcgctgctg gaggaattgc
     3661 ccagtgcggc ggcgcgtcag ctgatccagc tgctgacacc agaggaacgg cgaatcgcgc
     3721 agtcattgct cggttatccg gaaggcagcg ttggccgtct aatgacgccg gattttatcg
     3781 ccgtgcacga ggattggacc gtccagcagg tcctcgattt tgtgcgcgaa cacggccagg
     3841 atagcgagac gctcaacgtg gtctatgtcg tcgatgaccg ggggaaactg attgacgaca
     3901 tccgcatgcg cgagtttttg ctcaagccgc tcacaatcaa ggtgagcgac atccgggacc
     3961 aagttttcgt ggcattgagc gtgagcgatt cgcagcagga cgcactgaac atttttcgca
     4021 aatacgaccg caccgcgctg ccggtagttg attcgagggg ggtgctcgtc ggtatcgtga
     4081 ccatcgatga catgctcgac gtggcggaac aggaagcgac cgaagacatc caaaaattcg
     4141 gcggcatgac cgcactggac gagccttaca tgcggctgtc cctcacggaa atggtacgca
     4201 agcgcgcacc ctggctaatt attcttctgc tcggcgaaat gctcactgcc tcggccatgg
     4261 catattacga ggaagaactg gccaaggcgt tagtgctggc ggtgtttttg cctctcatta
     4321 tcgccagcgg tggcaactcc ggttcgcaag catctactct gatgattcga gccatggcac
     4381 tgggagaagt gaccctgcgc gactggtgga gcgtcatgcg ccgggaattt caggcgggat
     4441 taatcctggg cctttgtttg ggaacgattg gcgcgatccg cgtcggcgcc tggtcgatca
     4501 tcggcgaacg atattttcag cgcgaggtct atggtccaca ctgggctctc attgcgctga
     4561 ctgtgg
//
LOCUS       DASQUQ010000269         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1161124, whole genome shotgun sequence.
ACCESSION   DASQUQ010000269 DASQUQ010000000
VERSION     DASQUQ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161124"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VE486_07395"
     CDS             <1..246
                     /locus_tag="VE486_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38951.1"
                     /translation="TSKGNRMLMLQSTFAKMVRYHAAARGKGRRDWRRVNRMYGLLPN
                     VAQAEGFGDPIEEFVLYDFTLTNEKLNQNSTNETIGW"
     gene            428..1528
                     /locus_tag="VE486_07400"
     CDS             428..1528
                     /locus_tag="VE486_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38952.1"
                     /translation="MARKSRNGKKQNGEATISADSIEAAEDAGLRYVSDEQPGFSRRR
                     KGEEFEYFDTKEKPVRDEQRLLRIKRLAIPPAWSDVWICPSPNGHIQATGRDARRRKQ
                     YRYHERWREIRDENKYDRLINFGKALPKIRRRVQKDLALRGLPRAKVLATIVQLLERS
                     LIRVGNEEYARENKSFGLTTMQDRHVGVKGSKLRFRFRGKSGREHEVDVTDRRIARIV
                     SKLQDLPGQDLFQYLDDEGEVRDISSQDVNEYLREITGEDFSAKDFRTWAGTVLAAVA
                     LGSTGASKTKKQAKANIKDAIGAVAEILGNTAAICRKCYIHPDVLEAYLKGNCINGFK
                     TREELEPKGIDLASAQTAVLRFLQTSASEGSG"
     gene            complement(1593..>3765)
                     /locus_tag="VE486_07405"
     CDS             complement(1593..>3765)
                     /locus_tag="VE486_07405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZA38953.1"
                     /translation="SLSPSTKPFRMKYFSLSILSLTLAFTLIADLSAAELKSVDDFRA
                     AAAKANAVLTIPDWEQTPEAVEASMKDAIAKANTALDQIGAQDLGKVTFKSTVVALDD
                     LTYQAGLAANRAALIKETNTNSAMRSAGENAVKAFQEWAVGIDYREDVYKAIKAFADT
                     HPKLSGEDAKLLKETLRDYRRAGLDLAPDQRKEVEQLRKELSKLGTDFDTNIVKANAP
                     VIFTKADLDGMPESFFASPGIKTGDDAYTVMANVTWQFNTLQENAKSEATRKKLYVIR
                     ETLAKDTNVPVLNQMLALRNKIALRLGYKSWDDFQTEVKMARTGANAKKYISDLVIGI
                     QPKFDSEVAELQKLKAADTNDPKVRIDVWDWRYYSNQRNKQKYDVDKEALRAYFPFQK
                     VLDGMFNIYQNIFGLKFEKVAVPYKWIDDLQLYLVTDSATGEPLGMFYLDMFPREGKF
                     NHFAQFDIISGKLLPDGKYQRPTVALICNFPPANADKPSLLPHSDVETLFHEFGHALH
                     SIVTRARYGRFAGTHVPGDFVEAPSQMLQNWVWDKKVLDTFAADYRDPSKKIPAEIIK
                     KMNDAKLANAGVFYRRQFAFASLDLALHDQHAEDASYDCVAISNPILEKVFLPIDPST
                     TFVSYFGHLNGYDAGYYGYAWADAIAADMATVFEKAKDGYFDKQAGLRLRREIYEPGD
                     SRDVNISIEKFLGRKQSVQPFLKKIGINDKNASPTSSPATR"
BASE COUNT          857 a          939 c         1069 g          900 t
ORIGIN      
        1 acatccaaag gcaaccggat gctgatgctg cagagtacct tcgccaagat ggtgcggtat
       61 cacgctgcag ctcgcggaaa gggcagacgc gattggcgcc gcgtcaaccg aatgtacggc
      121 ttgctgccaa atgtcgccca agccgaagga ttcggagatc cgatcgagga gtttgttctt
      181 tacgacttta cgctcacgaa cgaaaagctg aaccagaaca gcacgaatga gacgatagga
      241 tggtaggtct aaccagacgc tgcagccaac cgctggccgg tccgatgttt acctttcaga
      301 tgacttcaac attgaactcc gtagcaaagc tcgctcccgc cagcggtggc tgagctccgt
      361 ctcgttagac gatcggcgct agcggtccat ttccgctttc tcatccgaat cacatcacgt
      421 gcgcgaattg gcaagaaagt cccgtaacgg gaagaagcaa aacggcgagg cgacgatcag
      481 tgctgattca attgaagcag cagaggacgc agggttacgc tatgtaagcg acgagcaacc
      541 agggttttcg cgtcgacgga agggcgagga attcgaatat ttcgatacca aggaaaagcc
      601 cgttcgtgac gagcaacgat tgctgcgcat caagcgtctc gccatcccgc cggcatggag
      661 tgatgtctgg atttgcccgt cgcctaatgg gcatattcaa gccaccggac gcgatgcgcg
      721 gagacggaaa cagtatcgat atcacgaacg ctggcgggaa atacgcgacg aaaacaagta
      781 cgatcgactg atcaatttcg gaaaagcgct gcctaaaatt cgcagacgcg tacagaaaga
      841 tttggctttg aggggtctgc ctcgggctaa agtgctcgcg acgatcgtgc agcttctcga
      901 gcgaagtctc atccgggttg gcaacgaaga gtacgcgcgc gaaaacaaat cgttcggtct
      961 gacgaccatg caggatcgac atgtgggcgt gaaaggctcg aagctgcgtt ttcgttttcg
     1021 cggcaagagt gggcgagagc atgaagtgga tgtaaccgat cgccggatcg cgcggattgt
     1081 atcgaaactg caggatttgc ccggccagga tctgtttcaa tacctcgatg acgagggtga
     1141 ggtgcgcgac atcagttccc aggatgtaaa cgagtatctg cgcgaaataa caggcgagga
     1201 ttttagcgcg aaagattttc gcacctgggc gggaacagtg ctggccgcgg ttgcgctggg
     1261 ctcgaccggc gcttccaaaa caaagaaaca ggccaaagcc aacatcaagg acgcaatcgg
     1321 tgcagtggca gagattcttg gcaacactgc ggccatttgt cggaagtgtt atattcaccc
     1381 tgatgttctc gaagcatatc tgaagggaaa ttgtatcaac gggtttaaga cccgcgaaga
     1441 gctcgaacct aaaggcattg atcttgcctc ggcacaaaca gccgttctga ggtttttgca
     1501 aaccagcgcg tcagaaggat ccggctgacg cggcctcgct ctttccccgt cgctcgcagg
     1561 tttccggaat ggcttttcag tctatccgag gttcacctgg ttgccggcga tgacgttggc
     1621 gacgcgtttt tgtcattgat cccgatcttc tttaggaatg gttggaccga ttgtttccgg
     1681 ccaaggaact tctcgatgga gatgttgacg tcgcgcgaat cgcccggctc gtaaatttcg
     1741 cggcgcaatc gtaatccggc ttgtttgtcg aagtaaccgt ccttggcttt ttcgaagacg
     1801 gtagccatat cggctgcgat ggcgtcggcc caggcataac cgtagtaacc ggcatcgtac
     1861 ccgtttaaat gaccaaagta ggaaacaaac gtcgtgctcg gatcgatggg cagaaaaact
     1921 ttttccagaa tcgggttcga tattgcgacg caatcgtaag acgcatcttc ggcgtgctga
     1981 tcgtgcagag cgagatcgag tgacgcgaac gcgaattggc gccggtagaa aacaccggcg
     2041 ttggccagct tggcgtcgtt cattttctta attatttctg ctggaatttt tttcgatgga
     2101 tcgcggtaat cggccgcgaa cgtgtcgagc accttcttgt cccagaccca gttttgcagc
     2161 atctgcgagg gcgcttcgac aaagtcacct gggacatgag ttccggcgaa acggccgtac
     2221 ctggcgcgag tgacaatcga atgcagagcg tgaccaaatt cgtggaacaa ggtttccacg
     2281 tcgctgtgcg gcaacaaaga tggtttgtct gcgttcgccg gtggaaaatt acagatcaat
     2341 gcaacggtgg gccgctggta tttgccatcc ggtaacagtt tgccgctgat aatatcgaac
     2401 tgcgcgaagt gattgaattt tccttcccgc ggaaacatgt cgagatagaa catcccgagt
     2461 ggctcgccgg tggcagagtc agtcacgaga taaagctgaa ggtcatcgat ccacttgtaa
     2521 ggcacggcga ccttttcgaa cttcaggccg aaaatgttct gatagatgtt gaacatccca
     2581 tcgagcacct tttggaatgg gaaataggcg cgcagagctt ccttatcgac gtcgtatttt
     2641 tgtttgtttc gctggttgct gtaatagcgc cagtcccaaa cgtcgatcct gaccttcgga
     2701 tcgttcgtat cggcggcttt cagtttttgt aattcggcca cttcgctgtc gaactttggc
     2761 tggattccaa tgacgagatc gctgatgtat ttcttcgcat tcgcgccggt cctggccatt
     2821 ttgacctcgg tctggaagtc gtcccatgat ttgtaaccca gccgcagggc aatcttgttc
     2881 cggagcgcca gcatttgatt gagaaccggg acgtttgtgt ctttcgcaag agtttcgcga
     2941 atgacgtaaa gttttttacg tgtggcttcg ctcttcgcgt tttcctgcag ggtattgaat
     3001 tgccaggtaa cgtttgccat tactgtgtaa gcgtcgtctc cggtcttgat gccaggcgag
     3061 gcgaagaaac tctccggcat gccgtcgaga tcggcctttg tgaatatgac cggcgcattg
     3121 gctttaacga tattcgtgtc gaaatcggtc ccgagtttgg ataattcctt gcgcagttgt
     3181 tcgacttctt tgcgctgatc gggtgccagg tcgaggccag cgcgccggta atcgcgcaat
     3241 gtttccttga gtagtttcgc atcttcgccc gagagcttcg gatgcgtatc ggcgaaagct
     3301 ttgatggctt tgtaaacgtc ctcgcgataa tcaattccaa ccgcccattc ttgaaacgcc
     3361 tttaccgcgt tctcgcccgc gctgcgcatc gcggagtttg tatttgtctc tttgatgagc
     3421 gccgccctat tcgccgcaag tccagcctga taggtgagat catcgagcgc gacgacggtg
     3481 cttttaaacg taactttgcc caaatcctgg gcgccgatct gatcgagagc ggtgttcgcc
     3541 ttggcgatcg catctttcat ggaagcctcg accgcttccg gtgtttgttc ccagtcggga
     3601 atggttaaga ccgcgttcgc ctttgccgca gcggcgcgaa aatcatcgac ggattttagt
     3661 tcagcggcgg aaagatcggc aatcagagta aaagcaagag tcagagagag gatcgacaag
     3721 gagaagtatt tcatgcggaa aggtttggtt gatggagaga gcgaa
//
LOCUS       DASQUQ010000270         3949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_76302, whole genome shotgun sequence.
ACCESSION   DASQUQ010000270 DASQUQ010000000
VERSION     DASQUQ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76302"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /gene="ilvC"
                     /locus_tag="VE486_07410"
     CDS             complement(<1..843)
                     /gene="ilvC"
                     /locus_tag="VE486_07410"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZA38954.1"
                     /translation="MAARIYTDKDGDLRWLKGKTCAVIGFGAQGRAHALNLRDSGIKV
                     MVGLYPGSKSRARARRSGLAVLGTAEAARRGDIIFLALSDTQMPVIYAEQIAPHLRPG
                     QTLLFAHGFAIHYPTIVPRKDVDVVMVAPKGLGPMVRREFVAGRGTPALIAIHQNPSR
                     HAKQTALAWAKGIGCTRAGVLETTFREETETDLFGEQAVLCGGTSALIRAGFDTLVRA
                     GYPAELAYFECLHELKFIVDLIHEAGIAGMRKLISDTARWGELTVGPRIIDRTVQRKM
                     RAALR"
     gene            complement(846..2627)
                     /locus_tag="VE486_07415"
     CDS             complement(846..2627)
                     /locus_tag="VE486_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZA38955.1"
                     /translation="MTETETQRQKEIRQAEELLFAGPQALGFAKGLFQGHFVADWVMP
                     YPRMPAAQQAELDKSLAELRQFLDEHLDAAEIDRQADIPRSVIDGLGRVGVLGATAPM
                     EFGGLGLTQMANCKLLEEIGRRCASTSVFVNAHHSIGIRALLLFGTHEQKQKWLPKLV
                     TGEQLGAFALTEQEAGSDAANVQMQARPSDDGTHFILSGEKRYITNAAIAHVLTVMAR
                     TPVPGKDKTAITAFLVTPDMPGFEILEARMPKMGIRGTATGRFALRDVKVPKENILGP
                     LGKGLKVALTVLDFGRTTFGACCTGAAKTCLRLAIEHANTRKQFNKTLGEFELVKKKI
                     ARIAADAYAMEAMTTVTGSFIDRGLEDYMIETAMLKVFTTERLWECINDVFQVHGGAA
                     YMTDLPLERMMRDARINQIGEGANEVLTSFIALVGMRGPGMEFKEIYDTMLKPFHGGM
                     SKAWAAGMNRLGAAIRVPDVPVQSEQLGARARELARLIWRFNFAVNRALITYREPILD
                     MQLVQERIAGAAMDLFASTCVLSRHDAEIQFARRNGDSAPPDHSAADLFLRQSFRRIR
                     RFLGGLTDNDDKSVLAAANSVLGKPRS"
     gene            complement(2801..>3949)
                     /locus_tag="VE486_07420"
     CDS             complement(2801..>3949)
                     /locus_tag="VE486_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38956.1"
                     /translation="GYNSEEIERIIAHIDAFDTIEDVTDTDGSTISSGLKPEHLSIFD
                     CAFEPFKGERSLSYMAHLRMMAAAQPFLSGAISKTVNMPEAATVDDIMNTYVEGWRLG
                     LKSIAIYRDGSKRSAPLNTRKTKDMGTTVAGIDDAGPDQFQKRILELEEELGTLRSRL
                     NQPVRHRMPDTRTSLTHRFEIAGHEGYITVGLYEDGQPGELFITMSKEGSTIGGLMDT
                     VGTLTSIALQYGVPLESLVKKFAYQRFEPSGFTKNPDIRHATSITDYVFRWLGCQFIK
                     GYREATSPNRTQPDLPLKEIAEIEKKALNRPVTHLARTGEKEVIDVITNHPSNSADGN
                     GNGHADRVMVALGSIFMEITCSVCGSDKVIRAGACGVCTECGTSQGCS"
BASE COUNT          786 a         1183 c         1090 g          890 t
ORIGIN      
        1 tcgcagagcc gctctcattt ttctttgcac ggttcgatcg ataattcttg ggccaacagt
       61 cagctccccc cacctcgcag tatcggaaat caatttgcgc atcccggcaa ttccggcttc
      121 gtggatgaga tccacaatga atttgagttc gtgcagacat tcgaaatagg ccagctcggc
      181 cggatagccg gcgcgaacaa gcgtatcaaa gcctgcgcga atcagcgcgc ttgtgccgcc
      241 gcaaagcacc gcttgttctc caaaaagatc ggtctccgtt tcttcccgaa aagtcgtctc
      301 cagtacgccg gcacgcgtgc aaccgattcc cttcgcccaa gccagcgcgg tttgcttcgc
      361 gtgccggctg ggattttgat ggatcgcaat cagggcaggc gtgccccggc ctgcgacaaa
      421 ttcccgacgc accatcggcc cgaggccttt gggcgcgacc atcaccacat cgacatcttt
      481 gcgtggaacg atcgtcggat aatgaatcgc aaagccatgc gcgaagagca gggtctgacc
      541 ggggcgcaaa tgcggcgcga tctgttctgc gtaaatgaca ggcatttgcg tgtctgaaag
      601 cgccagaaaa ataatgtccc cgcgccgcgc cgcttccgct gtcccgagca cggcgagacc
      661 gctccgtctg gcgcgcgccc gagacttgct accaggataa agcccaacca ttaccttaat
      721 cccgctgtct ctaagattga gagcgtgtgc gcggccttgc gcgccgaagc cgattaccgc
      781 gcatgttttt cctttcagcc agcggagatc gccgtccttg tcggtgtaaa tgcgagcggc
      841 caaatttagc tgcgtggctt acccaaaact gaatttgctg cggcgagcac cgatttgtcg
      901 tcgttatccg taagtccgcc gaggaagcga cgaattcggc gaaacgattg gcgcaagaac
      961 aggtcggcgg cggagtggtc gggtggcgcg gaatctccat ttcgacgcgc aaactggatt
     1021 tcggcgtcgt ggcgactgag cacgcaggtt gaggcaaaaa ggtccatggc cgcgccggcg
     1081 attcgctcct gaacaagttg catgtcgagg atcggttccc gataagtgat caacgcgcgg
     1141 ttgacggcaa aattgaaacg ccaaatcaaa cgagcgagtt cccgcgcccg cgcgccaagc
     1201 tgctcgctct gaacaggcac atcaggaaca cgaatcgctg cgccaagccg gttcattccc
     1261 gctgcccacg ccttgctcat ccccccgtgg aatggcttca gcatggtgtc gtaaatttcc
     1321 ttgaattcca ttcccgggcc gcgcattccc acgagcgcga tgaatgaagt caacacttcg
     1381 ttcgcccctt cgccgatctg attaatccgc gcgtcgcgca tcattcgttc cagcggaaga
     1441 tcggtcatat aggcggcgcc gccatgaacc tggaagacgt cgttgatgca ttcccacagc
     1501 cgctcggtcg tgaagacttt tagcatggcc gtctcgatca tgtaatcctc tagtccgcgg
     1561 tcgatgaacg atcccgtaac cgtcgtcatc gcttccatgg cataagcgtc ggccgcgatc
     1621 cgggcgattt tctttttgac cagctcgaat tcgcccagcg ttttgttgaa ttgtttgcgc
     1681 gtgttcgcgt gttcgatcgc cagccgtaaa cacgttttgg ccgcgcccgt gcaacacgcg
     1741 ccaaaggtgg ttcgtccaaa atcgagcacg gtcagggcga ctttcaggcc tttgcccagc
     1801 gggccgagaa tgttttcttt cgggactttg acatcgcgca gcgcgaatcg tcccgtagca
     1861 gtgccgcgga ttcccatctt cggcatgcgc gcctcgagga tttcgaatcc cggcatgtcg
     1921 ggcgtgacaa gaaatgccgt gatcgccgtt ttgtcctttc cgggcaccgg ggttcgtgcc
     1981 atcaccgtga gcacgtgcgc aatcgccgcg ttcgtgatgt aacgcttttc cccgctcaaa
     2041 atgaagtgtg tgccgtcgtc gctgggacgg gcctgcattt gcacgttcgc cgcatcggaa
     2101 ccggcctcct gctcggtcag agcgaatgca ccaagctgct cgccagttac tagcttcgga
     2161 agccatttct gcttttgttc gtgtgttcca aaaagcagca acgcacgaat tccgatggag
     2221 tgatgcgcat ttacgaaaac cgatgtcgat gcgcaacgac gcccgatttc ctccagcagc
     2281 ttgcagttag ccatctgcgt gaggcccagt ccgccgaatt ccattggcgc agttgctccg
     2341 agcacaccga cccgtcccag cccatcgatg acacttcgcg gaatgtcggc ttgtcgatct
     2401 atctcggcag catcgagatg ttcgtcgaga aattgccgga gctcggcaag cgacttgtcc
     2461 agctccgcct gttgcgccgc tggcatgcgc ggatacggca tcacccaatc ggcaacgaag
     2521 tggccctgga aaaggccttt cgcaaaaccc agcgcctgcg gacctgcaaa aagtagttcc
     2581 tcggcctgtc gaatttcctt ttgccgttgc gtttcagttt cggtcataaa aaagttctcc
     2641 gccggaaacg gcgggcgacg cactgtagcg aacacagcgg ccgtgtccac tcaaggagcg
     2701 gcggtttccc atccgccgag aggacttgga aatcgcgctt caccgaaacc gcatcgcagc
     2761 cgcgaatggc gatccagtgc gtgggctcag tcgatccctc tcagctacac ccctggctgg
     2821 tgccgcattc ggtacagacc ccgcaggcgc ccgcccggat cactttgtcg ctgccgcata
     2881 ctgagcaggt gatctccata aagatgctgc caagcgctac catgacgcgg tcggcatgac
     2941 cattgccatt tccatcggcg gaattggacg gatgatttgt gatcacgtcg attacttctt
     3001 tctcgccggt gcgcgccaaa tgcgttaccg gacggtttag agccttcttc tcgatctcgg
     3061 caatctcctt gagcggcaag tccggctgcg ttcggttcgg ggatgtcgcc tccctgtagc
     3121 ctttaatgaa ttggcatccc aaccagcgaa aaacgtaatc agtgatactg gtggcatgac
     3181 ggatatcggg gtttttcgtg aagccgctcg gctcgaatct ttggtaggcg aattttttga
     3241 cgagactctc cagtggcacg ccgtattgga gcgcaatcga agtgagcgtg ccgacggtat
     3301 ccattaaccc gccgatggtg cttccctcct tcgacatcgt gatgaaaagc tcgcctggct
     3361 ggccgtcttc gtacaaaccg actgtgatgt aaccttcgtg accagcgatt tcgaaccgat
     3421 gcgtcagcga cgtgcgtgta tcaggcatgc gatgccgaac cggttgattc agccggctgc
     3481 gcagcgttcc cagttcttct tcgagttcca aaatgcgctt ttgaaattgg tccggaccgg
     3541 catcatcgat accggctaca gttgtgccca tgtcttttgt cttgcgggtg ttcaggggcg
     3601 cggaccgttt cgagccatcg cgataaatcg cgatcgattt caagcccaat cgccaccctt
     3661 ccacgtaggt attcatgatg tcgtccacgg tggccgcctc cggcatgtta acagtcttgg
     3721 aaatcgcgcc gctcaggaag ggctgcgccg cggccatcat ccgcagatgc gccatataac
     3781 tcaaactgcg ttcgcctttg aatggctcga acgcgcaatc aaagatcgac aagtgctccg
     3841 gcttcaatcc ggatgaaatc gtcgatccat cggtgtcggt gacgtcttcg atggtgtcga
     3901 acgcgtcgat gtgcgcgatg atgcgttcga tctcttcgga attgtagcc
//
LOCUS       DASQUQ010000271         7530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_77767, whole genome shotgun sequence.
ACCESSION   DASQUQ010000271 DASQUQ010000000
VERSION     DASQUQ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77767"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VE486_07425"
     CDS             <1..862
                     /locus_tag="VE486_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZA38957.1"
                     /translation="ANGGSARERRPIFSGTKSFWGIAALAAVRDGLFKLDDPVSDTIT
                     EWKSDPRKSRITIRQLLNQTDGIEGASRLQRASIRDRNAMAIRLPAVAEPGSAFIYGP
                     SHIQVFAEVLRRKLNGRAATEYIAGRVLNRFGLRGLNYKKDARGNPLPATGFELTARE
                     WARLGELVLGRGNYRGRQIVSANLLREAFAGSQANPSYGLTFWLNEQAPLGREADVER
                     MLDLPWQSAGWADVCICRDAPADMVVALGSGYQRLFVISSLKAIIVRQGSNAKFSDAH
                     FLRLILGKGR"
     gene            complement(879..1850)
                     /locus_tag="VE486_07430"
     CDS             complement(879..1850)
                     /locus_tag="VE486_07430"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZA38958.1"
                     /translation="MRLTGTSATKTPRILLFAALSGIAAVVAENPNTSAPPAQRPLDT
                     SFNTRFAITLAAEVATPAGGEKKPDIHFVPTPQQLVDAMLEMARVNKDDIVYDLGSGD
                     GRLVITAAKKDGAHGIGIDIDPVRIREANENAKAAGVTDKVQFLQQDLFKSDFSKATV
                     ITLYLLDELNLRLRPHIFEQAKPGTRVVSHAFSMDNWEPDAEKTLQIEDKEYSAYYWV
                     VPANISGRWKVSGGNGADFPENVTVEQSFQKITARASSNGDILGEGKINGTLFTLTVN
                     QGSGSKNTQFHGKIEGNTIEAAAADDPDNRWCATREVGTEKPLDARN"
     gene            1912..2622
                     /locus_tag="VE486_07435"
     CDS             1912..2622
                     /locus_tag="VE486_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZA38959.1"
                     /translation="MASGFLIDWPAHLVDGLFFNLMNRFALGCGVVLAILLFIVVIAA
                     LALGGSYNRLVRLQQTVDQSWAQVQNVYQRRADLIPNLVNTVSGAANFEKSTLVEVTN
                     ARASVGRVQLDPNKAPADAAQLEQFQAAQGQLSNALSRLLLVVERYPELRANQNFLSL
                     QAQLEGTENRISVERGNFNTAVQNYNVAVRSFPTNLIAGMLGFPPRPFFTAQTGAERP
                     PPVNFNFGSPAPSPAATP"
     gene            2672..3481
                     /locus_tag="VE486_07440"
     CDS             2672..3481
                     /locus_tag="VE486_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZA38960.1"
                     /translation="MLNYGGEAVLVSIVRRSGRSTRLIPVVLLALAVQSVSAAEVIPP
                     KPAGYFNDYAGVVSKEAGLRFNEELAQFERETSNQIVVAIFPKMQSDSDIADYTQRVA
                     QAWGVGQKERRNGAVFFVFVQDRKMFIQVGYGLEGALPDATCFDITEFQVKPRFRAND
                     YEGGLAIGIDSILKAIRGEYKGSGKTVAEEHRGSGAPSLVFFIIFVIVLIVLSRVMRR
                     LGGYGYSSRRGGPIFVPMGGGGGWSSGGGGGFSGFSGGGGSFGGGGAGSSW"
     gene            3486..3923
                     /locus_tag="VE486_07445"
     CDS             3486..3923
                     /locus_tag="VE486_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZA38961.1"
                     /translation="MRTKEFLSKLEHDRIVNAIRAAESKTSGEIRVYIQRGKLSADPL
                     IAAEKKFRRLGMHKTRERNAALIFVAPRAHKFAVVGDGAIHDKCGEEFWHRVVDRMRE
                     HFQKEKFSEAIVEGIEEIGKLLSAYFPKTRANANELPDDIVER"
     gene            4243..5184
                     /locus_tag="VE486_07450"
                     /pseudo
     CDS             4243..5184
                     /locus_tag="VE486_07450"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
     gene            5287..5844
                     /gene="rplQ"
                     /locus_tag="VE486_07455"
     CDS             5287..5844
                     /gene="rplQ"
                     /locus_tag="VE486_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZA38962.1"
                     /translation="MRHQKKTIKLGRTAEHRKALLANQVCSLIEYQRIKTTLAKAKAV
                     RPLAEKMVTLGKSGSIHARRTALATLRQKNAVKKLFDDIAPRSAERNGGYTRIVKLGQ
                     RKSDSAPMAFIEWVDAAQVIEEKAADEKKAKRKEAEPKAKQKEPASQPEEPAIKEEES
                     APAPVEEEPKPKKRKWFGRKPAEEK"
     gene            complement(5864..6259)
                     /locus_tag="VE486_07460"
     CDS             complement(5864..6259)
                     /locus_tag="VE486_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38963.1"
                     /translation="MTTSLSERTAWHSIPTPSGRYTGDLDILVRAAPENAAKLVELLN
                     QFGFAHSGFRESDFTKPNQVIQLGRAPNRIDLLTSISGVTSEEAFGTRISAELDGIPV
                     FIIGKGALIRNKRALARRQDLADLETLEG"
     gene            6521..6895
                     /gene="ndhC"
                     /locus_tag="VE486_07465"
     CDS             6521..6895
                     /gene="ndhC"
                     /locus_tag="VE486_07465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZA38964.1"
                     /translation="MTPDPYLFIVIFVGVALVFPLIPLALAWLWRRFLQPPKPGAEKN
                     AIYECGVESIGEAQIQFRSQYYLYAIIFLIFDVEAIFLVPFAVAFTDLPVGAFVAILV
                     FLLLLIEGLVWAWGKGCLNWAR"
     gene            6944..>7530
                     /locus_tag="VE486_07470"
     CDS             6944..>7530
                     /locus_tag="VE486_07470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF011394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HZA38965.1"
                     /translation="MSEIDEGLRKELQRQGVWVTSMQELYNWGRKNSIWPLQFGLACC
                     AIEMIATAASRYDIARFGGEVFRPSPRQADLMIVAGTVTKKMAPQIVRLYNQMPDPKY
                     VISMGACAISGGPFKQGYNVLKGIDRYIPVDVYIPGCPPRPEALLHAFMELQRKIDSQ
                     KLTGDNKAEHLDPAEPSEFPVPSFGEHDLEPPANP"
BASE COUNT         1686 a         2025 c         2148 g         1671 t
ORIGIN      
        1 tgctaatgga ggatcggccc gcgaaagacg gccgatcttc agcgggacaa aaagcttttg
       61 gggaatcgcc gcgctcgcgg cggtgcgcga cggattattc aaactcgacg atcccgtctc
      121 cgacacgatc actgaatgga aaagcgatcc gcgcaaatcg cggatcacta ttcggcagtt
      181 gctcaaccag acggatggga ttgaaggcgc gtcgcgtctt cagcgcgcat cgatccgcga
      241 tcgaaatgcc atggcgattc gattgcccgc ggttgccgaa cccggctcgg ctttcatcta
      301 tggcccgagt catatccagg tttttgccga agtgctccgg cgaaaattga atgggcgcgc
      361 tgcgaccgag tacatcgcag gacgcgtgtt gaatcggttc gggcttcgtg ggctcaatta
      421 caagaaagat gcgcgcggta atccgctgcc cgcgaccggc tttgaattga ccgcccggga
      481 atgggcgcgg ctcggcgaac tggtccttgg gcgcggcaat tatcgcgggc ggcaaattgt
      541 ttcagcaaat ttattgcgcg aagcgtttgc cggatcgcag gcaaatccgt catatggcct
      601 tacattttgg ctaaatgagc aggcgccgct cggacgcgag gcagacgtgg aacgaatgct
      661 cgatcttccc tggcaaagcg cgggatgggc ggatgtttgc atctgtaggg acgcgcctgc
      721 cgatatggta gtggcgcttg gctcgggata tcagcgcctc ttcgtcatat cgtcgctgaa
      781 ggcaatcatt gtgcgccagg gatcaaacgc gaaattttcc gacgcccatt ttctgcggtt
      841 gatcttgggc aaaggtcgtt aaagcgcttt gcagcgattt aatttcgcgc gtccaatggc
      901 ttttcggtgc cgacttcacg tgtagcgcac catcggttat ctgggtcgtc agccgcagca
      961 gcctcgatgg tattcccttc gattttgccg tgaaattgtg tgtttttact gccagagcct
     1021 tggttcactg tgagcgtgaa cagggtcccg tttatctttc cctctcccag gatgtcgccg
     1081 ttgctactcg cccgagcagt gatcttctgg aagctttgtt ccaccgtcac attttccggg
     1141 aaatctgcgc cgttgccacc gctcaccttc cagcggccgc ttatgttagc cggcactacc
     1201 cagtaatagg cactgtattc cttatcttca atctgaagcg tcttctccgc gtccggttcc
     1261 caattatcca tactgaacgc gtgcgagact acgcgcgtac cgggtttcgc ctgctcaaaa
     1321 atgtgcgggc gcagcctgag gttcagttca tcgagcagat aaagcgtgat aacggtcgcc
     1381 ttgctaaagt cgcttttgaa gaggtcctgc tgaagaaact ggaccttgtc tgttacgccg
     1441 gctgcttttg cattttcgtt cgcctcgcgg atgcgcaccg gatcgatgtc aatgccgatg
     1501 ccgtgcgcgc cgtctttttt cgcagcggtg attaccaacc ggccatcacc cgaccccaga
     1561 tcgtaaacaa tgtcgtcttt gttgaccctt gccatctcga gcatcgcatc cacgagctgc
     1621 tgcggcgtcg gcacaaagtg aatgtcgggc ttcttttccc cgccggcggg tgtcgcgact
     1681 tccgccgcta gcgtgatcgc aaatcgagtg ttgaaggaag tgtcgagcgg ccgttgcgca
     1741 ggcggggcgc tggtgttcgg attttcggct accaccgccg cgatgccgga aagggccgcg
     1801 aagagcagga tgcgaggagt ctttgtggcg ctcgtaccgg tcaacctcat acaggtaaaa
     1861 cgtgacacgc taccctgtcg cgtgcatcag cgctctggtc tccgcagctt tgtggcaagc
     1921 ggcttcttga ttgactggcc ggcgcatctc gtggacggtt tgtttttcaa ccttatgaac
     1981 aggttcgctc ttggttgcgg cgtcgtgctc gccatcctgc tttttattgt cgtcattgca
     2041 gcgctcgcac tgggcggcag ttacaatcgc ctcgtccgtt tacagcaaac cgtcgatcaa
     2101 agttgggcgc aggtgcaaaa cgtctatcag cggcgcgccg atttgattcc gaatctggtg
     2161 aacacagttt cgggcgcggc caactttgaa aaatcaaccc tcgtcgaagt gaccaatgcg
     2221 cgtgccagcg tcggccgcgt gcagctcgat ccgaacaagg cgcctgccga tgcggcgcag
     2281 ctggagcaat tccaagcggc ccagggacaa ctgagcaacg cgctttcccg tttgctacta
     2341 gtggtcgagc gttatcccga actacgtgcg aaccagaatt ttctcagctt gcaggcacaa
     2401 ctcgaaggaa ccgagaatcg aatctcggtc gaacgcggga acttcaatac cgcagtccaa
     2461 aactacaacg ttgctgtgcg cagtttcccg accaatttga tcgcgggcat gctcggtttt
     2521 ccgccgcgtc catttttcac cgcgcaaaca ggcgcggaac ggccgccgcc ggtgaatttc
     2581 aattttggct cgccggcccc atcgcccgcc gcgacgccat aagtcattgc acgcgcggat
     2641 cgagacggtg gatcgagcag tcttttgctc gatgttaaac tatggcggcg aagccgtatt
     2701 agttagcatc gttcgacgga gcggtcgatc cacccggctg attcccgttg tgcttcttgc
     2761 cttagccgtt caatcggtaa gtgccgcgga agtcattccg ccaaaacccg ccgggtattt
     2821 caacgattac gccggcgttg tttcaaaaga agccgggctg cgtttcaacg aagagctcgc
     2881 ccaatttgag cgcgaaacgt cgaaccaaat cgtggtcgcg atcttcccga agatgcagag
     2941 cgactctgat atcgccgatt acacgcaacg tgtcgcgcaa gcatggggcg tcggtcagaa
     3001 ggagcggcgc aatggagctg tcttcttcgt cttcgttcaa gaccgcaaaa tgtttatcca
     3061 agtcggttac ggtttggaag gcgcgctgcc agatgccacc tgcttcgata taaccgaatt
     3121 ccaagtcaaa ccgcgatttc gagccaacga ctacgaggga ggcctggcca tcggaatcga
     3181 ttcgattctc aaagcaattc ggggcgaata caagggatcg ggaaaaaccg ttgccgaaga
     3241 acatcgtggc agtggcgctc caagcctcgt gttcttcatc atttttgtca ttgtcctgat
     3301 cgtcctttcg agggttatgc ggcgacttgg cggctacggc tattcatcac gccggggtgg
     3361 tccaattttt gtgccgatgg gtggtggagg aggttggtcg tctggaggcg gcggaggatt
     3421 cagtggtttt agcggcggag gcggcagttt cggcggaggc ggcgccggat cgagttggta
     3481 gccaaatgcg aacgaaagaa tttctcagca agctcgagca tgaccgaatt gtgaacgcga
     3541 ttcgggccgc cgaatcaaaa acgtcgggcg agattcgtgt ttatattcag cgcggcaagc
     3601 tgagtgcgga ccctttgatt gccgcagaga agaaatttcg ccggctcggt atgcataaga
     3661 cgcgggagcg caacgccgct ctcattttcg ttgcgccccg ggcgcataaa ttcgctgtcg
     3721 tcggcgacgg agcgattcat gacaaatgcg gcgaggaatt ctggcatcga gtcgtcgatc
     3781 gaatgcgcga gcattttcaa aaagagaaat tcagtgaggc catcgtcgaa ggcattgagg
     3841 agatcggtaa gctgctgagc gcgtattttc cgaagacgcg ggcaaacgca aatgaattgc
     3901 cggacgacat tgtcgaacgg tagggcgacg ctctgcggag ccgccgaatt cgtcggctcg
     3961 attgagtctc gccccagtaa aatcagctct tgccaaaccg tggattttgc ctttattccc
     4021 aacttcgcag cgagccttta gaattacgcc gttggtccct gggtctccgt cgcttgacgc
     4081 gaaaccgtac tcgccagggg gaacccggcg atccaagcga tgggggaaat ggagtattgg
     4141 agtggtgtag taatgatctt ttgtcgttgc tcattaatcc aatacgccag aactccatca
     4201 ctccaataga agattatgcc aatccgttac ggtcgttttg aaatgccgaa gaccctcgcc
     4261 aaagacgaga gcacagccac agatacttac gccaaattca ccgccgagcc gtttgaagcc
     4321 ggctatggac acacggtcgg aaattcgctc cggcgcgtcc tgctttcctc gctggaaggc
     4381 gcagccatca ctgcggtaaa aattaccggc gcgcaacatg aattcgccac gcttccgggc
     4441 gtggtcgagg acgtgaccga tatcgtcctc aacctcaaga aagtgaagtt caaagccgac
     4501 gatcatgaac cgagaaaact ttcgctgact gtaaacaagg aaggcgaagt cacggccggt
     4561 gagattcagg tggtgcaggg ctacgacgta ctcaatccca agcaggtgat ttgcacgctg
     4621 gacaagaaac ataaatttga cgccgagctg gaagtacgcg ttggccgcgg tttcgccacc
     4681 ggcgaggaaa acaagcggcc cgatcaaccg atcggcttaa ttccgatcga ctcgatcttt
     4741 tcaccggtca cgcgcgtgaa gtacgcagtc gagaatactc gagtcggtca gcgcaccgat
     4801 tacgataaac ttattctcga agtctggacc gacggacgcc tgactccgga tgacgcgttg
     4861 ctccaggcct cggcgattct gcggcatcac ctcgatgtat tcgtgaattt caacgaggaa
     4921 gtgatcgagt tcgacgagac gccggctggc gtgagcgaag aaaatctgaa gctcaagaaa
     4981 ctgctcaaca tgagtgtgaa tgagatcgag ctcagtgtgc gcgcggcgaa ttgcttaaat
     5041 aacgcgaata tcacgacggt cggtcagctc gcccaaaaaa ccgaggccga aatgctgaag
     5101 tatcgcaatt tcggcaagaa atcgctcaac gaaatcaaag acaagctaca acagcttggt
     5161 ttcagtctgg gaatgaaatt cgatccgggt cttgtggagc tgccggtgag cggtgatggt
     5221 gccagcccaa atgagtctgc tgccaaggaa taatttttcg atccgtcaaa ccatgttcgg
     5281 tgcgttatga gacaccagaa aaagacgatc aagctcggcc gcacggctga gcaccgcaaa
     5341 gcgctgctgg caaatcaggt ctgttccttg atcgaatatc aacgcatcaa gacaacattg
     5401 gccaaagcga aagcggttcg accgctggcc gaaaagatgg tgacccttgg caaaagcgga
     5461 tccattcatg cgcgccggac cgcactggcg accttgcggc aaaagaatgc tgtgaagaaa
     5521 ctcttcgacg acatcgcgcc gcggtcggcg gaacgcaacg gcggttacac gcgcattgtc
     5581 aagctcggcc aacggaagag cgactccgct cccatggcat ttattgagtg ggtggatgca
     5641 gcacaagtca tcgaggaaaa agcggcagac gaaaagaagg cgaaacgaaa agaggcggag
     5701 ccaaaggcaa agcagaagga gccggcttcg caaccggaag agccagcgat caaagaagaa
     5761 gaatcggcgc cagcacctgt cgaggaagag ccaaaaccga aaaagcgaaa atggttcggc
     5821 cggaagccgg ctgaagagaa gtaacttccg gctcgattgc gcgttagccc tccaacgtct
     5881 cgaggtcggc caaatcctgc cgccgggcga gtgctcgctt gtttcggatc aaggcgcctt
     5941 tgccaatgat gaaaacagga atgccatcaa gctccgcgga aatccttgtg ccaaaggcct
     6001 cctcgctggt cacgccgctg atgctggtta atagatcaat tcggttgggt gcgcgtccga
     6061 gctggataac ctggttgggc ttggtgaaat ccgattctcg gaacccggag tgggcgaacc
     6121 cgaattgatt caaaagttcg acaagcttcg ccgcattttc tggcgcggct cgaaccagaa
     6181 tatcgagatc accggtataa cgtcccgacg gagtcgggat ggaatgccag gctgtgcgct
     6241 ccgacaatga cgtagtcaac gccgcgcgag tttagtaatt cgagaaactc tctcaagtcg
     6301 ttgctcaggc gcatggttca acagttcaac cagaatttgc agccgttcgt tgcccgacag
     6361 ctttttgtaa aactcgcgat cagctttttc agcctcggtg aaactgcgaa atttccttac
     6421 cgttttgttc acgaccctaa actataaact tggcaacaac tctttccgag cgaatttgat
     6481 cgttgaaaca atttaactct tttaacttct aacgcgtcgg atgaccccag atccttacct
     6541 tttcatcgtc attttcgttg gggtcgcgct ggtttttcca ttgatcccgc tggccctggc
     6601 ctggctgtgg cgacggttcc tccagccgcc aaagccgggt gccgaaaaaa atgcgattta
     6661 cgaatgcggc gtcgagtcaa tcggcgaagc gcagatccag tttcggtcgc agtattatct
     6721 ttatgccatt attttcctga tcttcgacgt ggaagcgatt ttcctggtgc cgtttgcggt
     6781 ggccttcacc gatctgcccg tcggagcgtt cgtcgcgata ttggttttcc tcttgctcct
     6841 gattgaaggt ctcgtctggg cctggggaaa gggctgctta aattgggctc gctaacgcgg
     6901 tggttaaaag ggccaaaagg gttacattgt tacaacggaa acgatgagcg aaatcgacga
     6961 ggggcttcgc aaggaactgc aacgacaggg agtctgggtg acttcgatgc aggagcttta
     7021 caattgggga cggaaaaact cgatttggcc gctgcaattt gggctcgcct gctgcgcgat
     7081 cgaaatgatc gcaactgccg cctcgcgcta cgatattgcg cggtttgggg gcgaagtttt
     7141 ccggccatcg ccgcgtcagg cagatctgat gattgtcgcc ggcacggtga caaaaaaaat
     7201 ggcgccgcaa atcgtccggc tctacaacca gatgcccgat cccaagtacg tgatttcgat
     7261 gggggcgtgc gccatttccg gcgggccttt caagcaaggc tacaacgtgc tcaagggaat
     7321 cgaccgctac ataccggttg atgtttacat tcccggttgc ccgccgcggc cagaagcgct
     7381 gctgcacgca ttcatggaac tgcagcgcaa gatcgacagt caaaaactaa cgggcgacaa
     7441 caaggccgag catctcgatc cggcggaacc gagcgaattc ccggtgccat catttggcga
     7501 gcacgatttg gagccgccgg cgaatccgga
//
LOCUS       DASQUQ010000272         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_258243, whole genome shotgun sequence.
ACCESSION   DASQUQ010000272 DASQUQ010000000
VERSION     DASQUQ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258243"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VE486_07475"
     CDS             <1..1221
                     /locus_tag="VE486_07475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZA38966.1"
                     /translation="VIVLEREKFPRFHIGESLLPFSIQTFDRLGVREKLDRTFLPKFG
                     GEIMAPCGTRGMKFYFKDGYRSERDRAYQVTRSDFDKLLLDHSRENGAEVHEETEVKV
                     VNFVADGVKIDIENSNSARSTIEARYLLDCSGRQTTLGSFFNLKKTYDHLQKFSVFAH
                     YENIDRLPGRDATLIRMVRELDRWFWMIPLTDARTSVGVVMDTATFRATKLAPQAALE
                     KFIAEQPVMKERMKSAVRVSPVYSAGDYSYRNKKLFGDRWLLAGDAAGFIDPVFSSGV
                     FLAIMSAEKAADTLDEVLRDESLKPRLFKKYSRQVNYIMDIYLTFVNSWYRRSKEFLE
                     VFLNPTDTMQIAAAVNAVLAGNEGKSFEIRWRMWMFYFFVNAQRFLPFSPRLSLAPKM
                     EHLSSAPEPAGAAS"
     gene            1218..1718
                     /locus_tag="VE486_07480"
     CDS             1218..1718
                     /locus_tag="VE486_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38967.1"
                     /translation="MTKVGAIHQIARAARAFSPCLRAIAVNRPYFRITAILLAIVGLT
                     GCATTSRHQFVEPAGNWQTKSGQLMYRTANTTLVGDVLVRFSKSGDFELTVSKGPGVT
                     LLSLRQDASFAEVKGPFAEGRWSGPVEQAPKPLRGWLGLRDKLIHSNERSVRYTAGNE
                     TFLFRF"
     gene            complement(1783..>2006)
                     /locus_tag="VE486_07485"
     CDS             complement(1783..>2006)
                     /locus_tag="VE486_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38968.1"
                     /translation="RVAKSNAALPLNFWVADNLPSDENQNTGRERQDPHQDCRDGNVE
                     EHGDSNKDEIDRQQKHSEVFRDIHVSFLG"
BASE COUNT          455 a          553 c          542 g          456 t
ORIGIN      
        1 gtgatcgtgc tggaacggga aaaatttccg cgctttcaca tcggcgaatc gctgctgcct
       61 ttcagcattc aaacgtttga tcgccttggg gtgcgcgaaa agctggaccg cacatttctg
      121 ccaaagtttg gcggcgagat catggcccca tgcgggacga gggggatgaa gttttatttt
      181 aaggacggtt atcgctcaga gcgcgaccgc gcctatcaag tgacgcgttc ggatttcgat
      241 aaattattgc tcgaccattc acgcgagaat ggcgcggaag tccacgagga aaccgaagtg
      301 aaggtcgtca atttcgtcgc cgacggggtg aagatcgaca tcgaaaattc caacagcgcc
      361 agatcgacga tcgaagcgcg ctatctgctc gactgcagcg gaaggcaaac gacgctcggc
      421 agttttttca atctcaagaa gacgtacgac catctgcaga agttttcagt gttcgctcat
      481 tatgaaaata tcgatcgcct cccgggccgc gatgccacgc tcatccggat ggtgcgcgag
      541 ctcgatcgct ggttttggat gattccactg acagacgccc ggacaagtgt cggagttgtg
      601 atggacacgg ccacttttcg agcgaccaag ctcgcgcctc aggcagccct cgaaaaattc
      661 atcgccgagc agccggtgat gaaggaacga atgaagagcg ccgttcgcgt atcccccgtt
      721 tactccgccg gcgattattc ctatcggaat aaaaagctct ttggtgatcg ctggctcctg
      781 gccggcgacg ccgcgggatt catcgatccg gttttcagca gcggcgtgtt cttggccatc
      841 atgtcggccg aaaaagccgc cgacaccctc gacgaagtcc tgcgcgatga atcactcaag
      901 cctcgcctgt ttaagaaata ttcgcgccag gtcaactaca tcatggacat ttatcttacg
      961 ttcgtgaact cgtggtatcg ccgcagcaaa gaattcctcg aggtgtttct caatccgacg
     1021 gacacgatgc aaatcgccgc cgccgtcaac gccgtgctcg caggcaacga gggcaaaagt
     1081 ttcgagatca gatggcggat gtggatgttt tactttttcg tcaacgcgca acgctttctc
     1141 ccattttcgc cgcggctttc gctcgcaccg aaaatggaac atttgtcatc agcgcctgaa
     1201 cccgcaggcg cggcgtcatg acaaaggtag gggcgattca ccaaatcgcc cgcgccgcgc
     1261 gcgcgttctc gccctgtctt cgagcgattg cggtcaatcg cccctatttc agaatcacgg
     1321 cgatcttgct ggcgattgtt ggtctgaccg gctgtgcgac gacttcgcgg catcaatttg
     1381 ttgaaccggc aggcaattgg cagacgaaaa gcgggcagct catgtatcga acggcgaaca
     1441 cgacattggt cggcgatgtg ctggtccgtt tctcaaaaag cggtgatttc gaattgacgg
     1501 tttcaaaggg gccgggcgtg acgctgctat ctctacggca ggatgccagc tttgccgaag
     1561 tcaaagggcc attcgccgaa ggccgctggt caggtccagt cgaacaagcg ccaaaaccac
     1621 tgcgcggctg gctcggtttg cgcgacaaac tcattcactc gaacgagcga tcggttcgat
     1681 acactgccgg caacgaaact tttctgttcc gcttttagct aacacccccg ttcagcgcgg
     1741 tgatcatgcg gaaagtcttt ttaacgtgca aatgcgacgc gtttacccca agaaagaaac
     1801 atgaatatca cgaaaaacct cggaatgctt ctgctggcga tctatctcat ccttattgga
     1861 atcaccatgc tcttccacat tgccatcccg gcaatcctga tggggatctt ggcgctcgcg
     1921 gccggtattt tgattctcat cggaaggtag attatcagcc acccaaaagt ttagcggcaa
     1981 agccgcattt gatttggcaa cgcgtt
//
LOCUS       DASQUQ010000273         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_809482, whole genome shotgun sequence.
ACCESSION   DASQUQ010000273 DASQUQ010000000
VERSION     DASQUQ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809482"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VE486_07490"
     CDS             complement(<1..278)
                     /locus_tag="VE486_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZA38969.1"
                     /translation="MKLSRTKEYNFGWLVLLGLVDVAFLLIFFLLLSSNFILQPGISV
                     SMPFSRFALGPQVNQQIISVTGGTAPAIYFRDQKITLAQLGPLLDAARR"
     gene            complement(378..1061)
                     /locus_tag="VE486_07495"
     CDS             complement(378..1061)
                     /locus_tag="VE486_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZA38970.1"
                     /translation="MIDYIQKGGLLMWPILACSIISIAIFAERLLYLHRATIHVGEFL
                     KGLSNLIRRRNFAEALHESAGTPGPVARVIHSAIIQHDAPRAELRDIVQEAAQLEVPR
                     LERFLGVLATIAFLAPLLGLLGTVAGMIDAFGTIASSGGYASVTELSSGVYKSLLTTA
                     AGLVVATPTFVAYSYLSSRVNTMMHDMERAGIEIVHMLTESRPVSGIISFQPSGDASA
                     REREARQDL"
     gene            complement(1144..1659)
                     /locus_tag="VE486_07500"
     CDS             complement(1144..1659)
                     /locus_tag="VE486_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38971.1"
                     /translation="MKVFAVGCAIVSLALACGANIALSQDTEKTEINAARVTVSMNAD
                     GSRTVYQFDDAQRKAIATTTGQDGKLRQKIRYELDDAGRFSSGRIFGSDGRLRLKSRY
                     TYDNAGRLKEETQSAEDGTLLHKIVYSYDPNGKQTGYSIFDASGKLLGRTTPLTTAPS
                     SSPKSREKSSR"
     gene            1786..2241
                     /locus_tag="VE486_07505"
     CDS             1786..2241
                     /locus_tag="VE486_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38972.1"
                     /translation="MDASSPPERFYAGEFRHSIDEKNRITIPSRWRGDKAENFILLPE
                     ASQQFLLVMSPAEFGRMSAVAESSESVPARDRRVFLRQLHSRAQHASADRQGRLVLPD
                     ELCRKVELKGEVALVGGRGRFEIWNLQRWKRAHEEETPTYQHVANLIGL"
     gene            2231..>3246
                     /gene="rsmH"
                     /locus_tag="VE486_07510"
     CDS             2231..>3246
                     /gene="rsmH"
                     /locus_tag="VE486_07510"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZA38973.1"
                     /translation="MAYNQMLGQSAINTFWFERSIWSAIDPLTIAEEEAHEMEDFIYH
                     RPVLEREVVELLAAGPGSLIVDGTCGGGGHTEALLSAGADVLALDQDPDAVEHVSKQL
                     TRFGRRVAVRQANFRHANKVLDELGIRAIGGALLDLGVSAWQLEDAKRGFSFMRNGPL
                     DMRMDPRNQLTAATIVNEYSEEQLTRLFRELGEEPAARRIASLIVKMRKTAPFRETLP
                     LVRAIEKVVGRRGRRHPATQVFQALRMEVNDELGALEEGLRVLTARLESGARVGVITF
                     HSLEDRTVKNFFRDRSREWLDQPEWPEPRPNPDYDLKVVTSKPVEPTEEEQRANPRSR
                     SAKLR"
BASE COUNT          743 a          874 c          938 g          691 t
ORIGIN      
        1 cgccttgccg cgtccagcaa cggcccaagt tgtgcgagcg tgatcttttg gtcccggaaa
       61 taaatagccg gagctgtgcc cccggtaacg ctgatgattt gctggttaac ctgggggcca
      121 agcgcaaaac gcgagaacgg catcgaaacg gaaatgcccg gctgcaaaat aaaattcgag
      181 ctgagcaaca gaaaaaaaat cagtaggaaa gcaacatcga ccaaccccag caggacaagc
      241 cagccgaaat tatattcctt cgtccggcta agcttcatga atgaggaagt ttcaggtgtt
      301 aagtttcaag tcttaagtgc tgggtcatgg gtcgcggact gctcaacact taaaactgaa
      361 acttgaaact cttctagcta aagatcctga cgcgcttccc gctcgcgcgc agatgcatcc
      421 ccggacggct ggaagctgat aatgccgctg accgggcggc tctccgtcaa catgtggacg
      481 atctcaatcc cggcgcgctc catgtcgtgc atcatggtgt tcacgcgcga agaaaggtag
      541 ctgtatgcga caaacgttgg cgtggccacc accaacccgg ccgcggtcgt cagcaaactc
      601 ttgtaaacgc cgctggaaag ctcggtcacc gatgcataac cgccactgga cgcgatcgtc
      661 ccaaatgcgt cgatcattcc cgcaactgtg ccaagcaaac ccagcaaggg cgcaaggaaa
      721 gcgattgtgg ccagcactcc cagaaatcgc tcgagcctgg gcacctcgag ctgggccgct
      781 tcctggacaa tgtcgcgcaa ttctgcgcga ggcgcatcgt gctggatgat ggcggaatga
      841 atcacccgcg ctaccgggcc cggtgttcca gccgactcat gtagcgcttc ggcgaaatta
      901 cggcgccgaa tcagattgga aagtcccttc aagaattcgc cgacgtgaat ggtcgctcga
      961 tgcaggtaga gcaggcgctc ggcgaaaata gcgatcgaaa tgatgctaca ggccagaata
     1021 ggccacatta atagtccgcc tttttggatg taatcaatca cgtccgtttt ttagccttct
     1081 ttggcaattg tggcaagctt agagcttgcg ggaagcgaca ggcctgcttc gtgaatatca
     1141 cccctaccgt gaacttttct cgcgcgattt tggcgaagac gatggggcgg tggtcagggg
     1201 agtcgtgcgg cccagcaact tgccggacgc atcgaaaatg gaatagccgg tttgcttgcc
     1261 gtttggatcg taactgtaaa cgatcttgtg caggagcgtc ccgtcttcgg cgctttgcgt
     1321 ttcttccttg agacgccccg cattgtcgta agtatatcgg ctctttagcc gaaggcggcc
     1381 atcggatccg aaaattcggc cgctggaaaa acgcccggca tcatccaatt cataacgaat
     1441 tttctggcgg agtttaccat cctgtccggt ggttgtggca atggctttgc gctgcgcgtc
     1501 atcgaattgg taaacggtgc gcgaaccatc ggcgttcatc gacacggtca ctcgcgcagc
     1561 gttgatctca gttttctcgg tgtcttggct cagggcgata ttagcgccac aggcgagggc
     1621 cagggaaacg atggcgcatc caaccgcgaa aaccttcata acttgaggag cttacggttg
     1681 cggaaaaagc atgcaaatgg gcaaaaattt ttattgaccg cggattgggg aggtggttaa
     1741 agatggggac aagtggggta aaatggatga atcctccacc gcaatatgga cgcctcttct
     1801 ccacctgaac gtttctacgc cggagaattt cgccattcca tcgacgagaa aaatcgcatc
     1861 acaattccgt cccgttggcg gggagacaag gccgaaaatt tcatcctttt gccagaggcg
     1921 agtcagcaat ttttgctcgt gatgtcgcca gcggaatttg gccggatgag cgcagtggca
     1981 gaatcgagcg agagtgttcc cgcgcgtgat cgccgggttt tcctgcgcca gctacattcc
     2041 cgcgctcagc acgcgagcgc ggatcggcaa gggcgcctcg tcctgccgga cgaactgtgc
     2101 agaaaggtcg agttaaaagg tgaagtcgcc ctcgtcggcg gccgcgggcg ttttgagatt
     2161 tggaatttgc aacgctggaa acgggcgcac gaagaggaaa cgcccactta tcaacacgtg
     2221 gcgaatttaa ttggcttata atcagatgct tggtcaaagc gctatcaaca cattttggtt
     2281 tgagcgatcc atctggtcgg ccattgatcc gttgactatt gccgaagagg aggcgcacga
     2341 aatggaagac ttcatttatc accgtcccgt gctcgaaagg gaggttgtcg agctgctcgc
     2401 ggccggacct ggctcactca ttgtcgatgg gacctgtggc ggtggcggcc acacggaagc
     2461 gcttttgagt gccggcgcgg atgttctcgc cctggatcag gaccctgatg ctgtcgaaca
     2521 tgtgagcaag caactgacac gcttcggtcg gcgcgtcgcc gtacgacaag cgaattttcg
     2581 gcacgcgaat aaagttctcg acgagctcgg aattcgtgcc atcggcggag cgcttctcga
     2641 tctcggtgtc tctgcgtggc aactcgaaga tgcgaagcgc ggttttagtt tcatgcgcaa
     2701 cggcccgctt gacatgcgca tggacccacg gaaccagctg actgctgcca cgatcgtaaa
     2761 tgagtacagc gaggagcaat tgacccggtt gttccgcgag ctgggggaag aaccagctgc
     2821 gcgtcgtatc gcaagcttga tcgtaaagat gcggaaaaca gctccgttcc gcgagactct
     2881 tccgctggtg cgtgcgattg aaaaagttgt cggacgtcgc ggccggcgac atccggccac
     2941 gcaagttttt caagcgctgc gaatggaagt gaatgatgaa cttggcgccc tcgaagaagg
     3001 gctgcgcgtt ttgactgcgc gactcgagtc cggcgcgcgc gtcggcgtca tcacttttca
     3061 ttctttggaa gatcggaccg taaaaaactt tttccgcgat cgcagccggg aatggctcga
     3121 ccagccggaa tggcccgaac cacggcctaa tcccgattac gacctcaagg tggttacctc
     3181 caaaccggtt gagccgactg aagaagaaca acgcgccaat ccccgttcgc ggagcgcaaa
     3241 gctgcg
//
LOCUS       DASQUQ010000274         6525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_1164950, whole genome shotgun sequence.
ACCESSION   DASQUQ010000274 DASQUQ010000000
VERSION     DASQUQ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164950"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1550
                     /gene="nagA"
                     /locus_tag="VE486_07515"
     CDS             213..1550
                     /gene="nagA"
                     /locus_tag="VE486_07515"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HZA38974.1"
                     /translation="MNVEFSGTFRRLDGVSPYQHMILTNARLIFPDGIRDGLEVVVEG
                     GRITAIREQTRLRSPRRPTARQTHARGNDVIHLGGNYLAPGFVDLHVHGAAGRDTMEA
                     SPEAFGVICDYHASGGTTSLLLTTATAPMNSIVDVLTAVRDSGQRRARQLALSLPNGR
                     PTSAIAGVHVEGPFISKAKCGAQRAEFIRDPSATVVQQLLAYADAIKRVTIAPELPGA
                     LEAIESFHAIGISVSGGHSDAWDEDAQNAFDRGMRSVTHTFNCMSSARRRGIYRVAGL
                     LEFALSESQISCELIADGHHVSARLMKMLYRAKGASGICLVTDATAGAGLPDGSRFSL
                     FGKDCIVEGGVCLLADRSAMAGSVSRMIDLVRMMVQHVGVPLHEAVTMATENPARAVG
                     LQAKGHLEIGADADFVVLSPELEVVRTFSAGEEIFSLDRQSVIPSGVEGSLNV"
     gene            complement(1621..2709)
                     /locus_tag="VE486_07520"
     CDS             complement(1621..2709)
                     /locus_tag="VE486_07520"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZA38975.1"
                     /translation="MKKRILILTAGFGEGHNSAARGVRDGLARVAPGQVESELRDLFP
                     EAYGAFNEVVRRTYLAVINRWPRSWGYIYRWLDGITNFDERFQRFSRLKQHFTRVVER
                     FQPDVVVSTFPPYPYLLRQIFGANHRCKSVVVITDSITVNAIWYRCDADYFLAPNEQS
                     AVVLRAAGVASEKIKTFGFPVSPKFADLTQDRPLPTSNFRRRVLYMIHAATRGAPELV
                     RQLAGLGIDLTVTIGRAEKLRPEIEEAAGDSKTEILGWTDELPRMLHESHLLIGKAGG
                     ATVQETIAAGCPMIINHVVAGQEEGNARLIVETNSGVIARSPGEVAAQVQRAFADDAK
                     QWREWAANISRLSRPRASLDIADFLLSI"
     gene            2792..3685
                     /locus_tag="VE486_07525"
     CDS             2792..3685
                     /locus_tag="VE486_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZA38976.1"
                     /translation="MKYLRFIWRVLRRMVWETAMDETTGLAAQMAYLLLFALAPGLLF
                     LWHLLGLFGTDPTKLHQMFVILQSFMPPDPKVQDILDTAMASVVVTGSSGLLANVGII
                     LGIYFGTIFIATISRALSHTHGVQEDPHWWSKYIVSFFMLFWFGIVLLFCFNAIVFGE
                     KLAGIAEVNLQLAVPLQAWIAALNLPFTGLALIFLALTLYLLTPENYLTVRQALPGAI
                     FFSLGWILATKLFQYYVARYDRYNPTYLALASIIVLLTWMYLTCLLLLLGGKLNTILQ
                     RERERMRRAVSSAPAPAAQTP"
     gene            complement(3843..4469)
                     /locus_tag="VE486_07530"
     CDS             complement(3843..4469)
                     /locus_tag="VE486_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38977.1"
                     /translation="MPDFSITTCARSSAGAARGTFARGDTSGVGTAIARGGVGVDSSD
                     GAGDFSEGGVGVFSGSGVSFSFLVAFAFTAFLPPDDALFFLDFFFFAFGFGVGLGDFF
                     DFFAARVGSGPAVAGLDFGFGVASSSSSDSFADFDLGAGDSSGVGDASVSLVASPAAS
                     FAFGIGLGDSLGVGDALRFFFDLLAPALALATGLGDFAGVGLSSSSPT"
     gene            4450..5055
                     /locus_tag="VE486_07535"
     CDS             4450..5055
                     /locus_tag="VE486_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HZA38978.1"
                     /translation="MIEKSGIEEDQGFEPPPSPPPRRGFWPWSLGRSREYRYLTRSVI
                     EAIRRAPVKRRRWQFIVVHNSGTRQGNARAFDYYHRKVRRMQNGLAYHFVIGNGTSSG
                     NGQIEIGDRWRRQINGGHVHSDYLNNISLGICLVGDFNRDQPTRAQLESCEELIRYLR
                     ERCGKVGLRSIVVRPHREMNPPRWATDCPGDDFPYSWFRRF"
     gene            complement(5180..6319)
                     /locus_tag="VE486_07540"
     CDS             complement(5180..6319)
                     /locus_tag="VE486_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38979.1"
                     /translation="MKKNFFGKAVTTIALSILIGAFGVSAQENPEIAPRALPVSAGPN
                     DIARFLAGMPVPPDSPLAPLTQDPAWQAHSAYFEKEFSKLSVRQLQKLHAWEDIYLPE
                     ATQPIPVAFYMFSGPDFLYVDQFFPRASVYILCGKESMGPPPDPLRIPNLSGALGNLE
                     NAMKSSLSTTYFITKNMKIDLHEQNLNGVLPILYTFIARADKSITSVTFGSLNGTGTF
                     QESAPGKKGGSIPGVRINYADNQTGASQTVYYFTTDISDGGIKSTPGFLKFCERFGVG
                     SSFLKSSSYLMFEEGFATIRNFILTRSNRIVQDDSGIPLAYFDPGKWNLRLFGNYLGP
                     IELFKQHYQPRLQELFAQSNPPPIDFGFGYRWNYREANLMVAERK"
     gene            complement(6316..>6525)
                     /locus_tag="VE486_07545"
     CDS             complement(6316..>6525)
                     /locus_tag="VE486_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38980.1"
                     /translation="VLRFTHPKMMRPYRVWAYPLPPILFSIITIWMMFYLLRWHSRES
                     LAGLGTAFFGLLLYFCARKRHGHST"
BASE COUNT         1433 a         1797 c         1869 g         1426 t
ORIGIN      
        1 aggcaattcc agcggagaac gaccaccaga aatcacatcg acttcccagc gcactccgct
       61 tccggagagc acaggccgct agcctacagt tcgcgacagc ttgccgcgac ctgcctttta
      121 ccacttcacg tggttatgat gccaggcatt cggccagcgg cctgcgctcc ccagaagcgc
      181 attgaaagtg ttccgcgcct tgtgatggcg ggttgaacgt tgaattttct ggcacatttc
      241 gtcggctcga cggagtctcg ccttaccagc acatgatcct cacaaacgcg cgtttgattt
      301 ttccagatgg gattcgcgat ggcctcgaag tcgtcgtcga aggaggaaga attacggcaa
      361 tacgcgagca aacccgcctt cggtcgccgc ggcgacctac ggcgcggcag acgcacgcgc
      421 gcgggaacga cgttatccat cttggcggaa actatctcgc acctggattt gtcgatcttc
      481 acgtccacgg cgcggccggt cgcgacacga tggaggcatc gccggaagcg tttggcgtaa
      541 tctgcgatta tcatgcgagt ggcggcacga cctcactttt gcttacgacg gcgacggcgc
      601 cgatgaacag tatcgtcgat gttctaaccg cagtgcgtga ttctggccaa cggcgcgccc
      661 ggcagcttgc cctgagcctg ccgaacgggc gtcctaccag cgcgattgcc ggcgtccatg
      721 tcgaagggcc gttcatttca aaagcgaaat gcggcgcaca acgcgcggaa ttcattcggg
      781 acccgtcagc aaccgtcgtg cagcaattgc ttgcgtacgc ggatgcgatc aaacgcgtca
      841 caattgcgcc ggagctgccg ggagcccttg aagcgatcga aagttttcat gcgatcggaa
      901 tcagcgtcag cggcggtcat agcgatgcat gggacgagga cgcccagaac gcgttcgatc
      961 gcggcatgcg cagcgtcaca cacacattta attgcatgtc gagcgcccgt cgtcgtggca
     1021 tttatcgcgt cgccggtttg ctcgaatttg ccttaagcga gtcgcagatc agttgcgaac
     1081 tgattgcgga cggtcatcac gtttctgcgc ggttgatgaa aatgttgtat cgcgcgaaag
     1141 gcgccagtgg gatttgtctg gtgaccgatg caactgccgg cgcgggattg cccgatggat
     1201 ctcgattctc gctgttcggt aaggattgca tcgtggaagg cggcgtctgt ttactggccg
     1261 accgctcggc gatggctggc agcgtctcgc gcatgatcga tctcgtccga atgatggtgc
     1321 aacacgtcgg ggttccactg cacgaagcgg tcacaatggc gacagagaat ccagcgcgcg
     1381 ccgttggtct ccaagccaag ggccatctgg aaattggcgc agacgcagat ttcgtcgtgc
     1441 tgtctccaga actggaagtg gtgcggacat tttccgccgg tgaagaaatt ttttcgttag
     1501 atcgacaatc tgtcatcccg agcggagtcg agggatctct caatgtttaa agatgtctcc
     1561 cgaccttcgc atgaagctac ggcgcggcac gtcacttcgg tcgacatgac aaacccggcg
     1621 ctagatcgac aataaaaagt cagcgatatc gagcgaggcc cgcggcctgc tcaaacggct
     1681 gatatttgcg gcccattcgc gccattgttt tgcgtcatcg gcaaatgccc gctgaacctg
     1741 cgcggccact tccccggggg aacgcgcgat cacgcctgag tttgtttcga cgattagccg
     1801 ggcgttgcct tcttcctggc cagccacgac gtgattaatg atcatcgggc aaccggccgc
     1861 gatcgtttcc tgcacggtcg cgccgccagc tttgccaatg agcaaatggc tttcgtgaag
     1921 catgcgcggt agttcgtccg tccatccgag aatctctgtc ttgctatcac ctgctgcctc
     1981 ttcaatctcc ggccgcagtt tttcggcgcg accgattgtc accgtcagat cgatgccaag
     2041 gccagcaagc tgccgcacaa gttccggtgc gccgcgcgtc gcggcatgga tcatgtacaa
     2101 aacgcggcgg cggaagttgc tggttggcag gggtcgatcc tgcgtcaagt cagcaaattt
     2161 tgggctcaca ggaaatccga atgttttgat tttttccgac gcaactccgg ctgcccgcaa
     2221 caccacggcg ctctgttcat tgggcgcaag gaaatagtcg gcatcgcagc ggtaccaaat
     2281 agcgttcacg gtaatcgaat cggtgataac gactacgctt ttgcagcggt gattcgcccc
     2341 gaaaatttgg cgaagcaagt acggataagg cggaaaagtg gagacgacca catcgggttg
     2401 aaaacgttct accacgcgtg tgaaatgctg tttcaggcgt gagaagcgtt gaaatcgttc
     2461 gtcgaagttt gtgatgccat cgagccaacg gtaaatgtat ccccaggagc gaggccaacg
     2521 attgatcacc gcaaggtagg tacggcggac aacttcatta aaagcgccat aagcttcggg
     2581 aaataagtct cgcaattcgc tctcgacttg acctggggcc acacgcgcca gtccgtcccg
     2641 gactccacgg gcagcgctgt tgtgaccttc tccaaaaccc gcggtgagga tcaggattct
     2701 ttttttcaca gctcgttagg cgatacagcg gaagagaagc ggacgaagtt taatggatat
     2761 ttgtacccgg caaagtaaac tctcgcggga gatgaaatat ctcaggttta tttggcgcgt
     2821 tttgcgccgc atggtttggg aaaccgcgat ggatgagacg accggcctcg ctgcgcagat
     2881 ggcgtatctg cttctttttg cgctcgcgcc cgggctgctt tttctctggc atctgctcgg
     2941 tctcttcggc accgatccaa ccaaactcca ccagatgttt gttatcttgc aaagcttcat
     3001 gccaccggac ccaaaggtgc aggatattct cgataccgcg atggccagcg tggtggtgac
     3061 gggttcgagc ggcctcctgg caaacgtcgg cattatcctt gggatttatt ttggcacgat
     3121 atttatcgcc acgatttccc gggcattgag ccatacgcat ggcgtgcagg aagatccaca
     3181 ttggtggtcg aaatatattg tctcgttttt catgctcttc tggttcggca tcgtcctgct
     3241 gttttgcttt aacgccatcg tcttcggcga aaaactcgcc gggattgcgg aagtcaatct
     3301 tcagctcgca gtcccgttgc aggcatggat cgcggcgctc aacctgccgt tcaccgggct
     3361 ggcgctgatt tttcttgcac tcacccttta tctcctgacc ccggaaaatt atctcaccgt
     3421 ccgccaggcg ttgccgggtg cgattttctt ttctctcggt tggattctgg ctaccaagct
     3481 ctttcaatat tatgtcgcaa gatacgatcg gtacaacccg acttatctcg cgctggcgtc
     3541 catcattgtg cttcttacct ggatgtatct gacctgtctg ctacttcttc tcggtggcaa
     3601 attgaacacc attctccagc gcgaacgcga gcggatgcgc agggctgtct cttccgcgcc
     3661 cgcgcctgcc gcgcagacac cctgacttcg ggttttgaat tttagttatt cggattcgtt
     3721 tgggatttcc ctcgcttgat cgcgtcgcgc tctctgttac gctccgtcgc agtggtgcgc
     3781 tttcgccttt ggctcgcaac tgcactcgcg ctcagcgcga tcgccggcag tctccttcgc
     3841 gctcaagtcg gtgacgacga ggaaagcccg acaccagcaa aatcgcccag tccggttgcc
     3901 aaagccaaag cgggtgcaag caagtcgaag aagaaacgga gcgcgtcccc cacgcccagg
     3961 gaatcaccga gcccgatccc gaaggcgaaa ctggcagcag gagaggcaac gagcgagacc
     4021 gaagcatcgc cgacgccgga cgaatcgccg gctcccaaat cgaaatcggc gaaagaatct
     4081 gacgacgaag aggaggcaac cccaaagcca aaatcgagcc ccgcgactgc ggggcccgaa
     4141 ccaactctgg ctgcaaagaa atcgaaaaaa tctccgagcc cgacgccaaa accaaaagcg
     4201 aagaagaaaa aatcgagaaa gaaaagcgcg tcgtcgggag gcaaaaaagc tgtgaaggcg
     4261 aaagcaacca gaaaagaaaa agagacgcca gaaccggaaa agaccccgac gccgccttcc
     4321 gaaaaatctc ctgcgccatc tgaagaatcg accccaacgc cgccacgggc gattgcggtg
     4381 ccgactccag acgtatcgcc gcgggcaaag gttccacgcg ctgctcctgc ggaagagcgg
     4441 gcacaagtcg tgatcgagaa atcaggcatc gaggaagacc aggggtttga accacctcca
     4501 tctccgccgc cgcgcagagg tttttggcca tggtcgctag gccgttcgag agagtatcgc
     4561 tatctcacgc gttcggtcat cgaagcaatt cgccgcgcgc cagtaaagcg gcgtcgctgg
     4621 caatttatcg tcgtgcacaa tagtgggacg cggcagggaa acgcgcgcgc gttcgattat
     4681 tatcatcgaa aggtgcggcg gatgcagaat ggcctcgcct atcatttcgt gatcggcaac
     4741 ggaacatcga gcggcaacgg gcagatcgag ataggggacc gctggcgccg ccaaatcaat
     4801 ggcgggcatg tgcacagcga ctacttgaac aacatctcgc tcggaatttg tctggtagga
     4861 gactttaatc gcgaccagcc cacccgcgcg cagcttgaat cgtgcgaaga actgattcgc
     4921 tacctgcgag agcgctgcgg caaggttggc ctgcgcagca tcgtggtgcg gccgcatcgt
     4981 gaaatgaatc cgccgcgctg ggctactgat tgtcccggcg atgattttcc atactcctgg
     5041 ttccgaagat tttagccgct gtgattcgtg cgctctgtca tgtcgagcgg agtcgagaca
     5101 tctcactctt ttccattgtc accttggacg agactagcag tgagagattc ctcgacttgg
     5161 ctcggaatga caaaagatat cacttgcgct ctgccaccat caaattggct tccctgtagt
     5221 tccagcgata gccgaatccg aagtcgattg gtggtggatt gctttgggcg aagagttcct
     5281 ggagtctcgg ctgataatgt tgtttgaaga gctcgatcgg cccgagataa ttgccgaaca
     5341 ggcgcaggtt ccacttgccc gggtcaaagt acgccagcgg gattccggaa tcgtcctgta
     5401 caattcggtt gctgcgcgtg agaatgaaat tgcggatcgt ggcgaaacct tcctcgaaca
     5461 tcagatacga ggaagatttg aggaaactcg agccgacgcc gaatcgctcg caaaatttca
     5521 agaaacccgg agtggatttg attccgccat cggagatatc ggtggtgaaa taatatactg
     5581 tttgtgaagc gcccgtttgg ttgtcggcat agttgatccg cactccgggg atgctgccgc
     5641 ctttctttcc gggcgcgctt tcctgaaacg tgccggtgcc atttaaagag ccgaacgtga
     5701 cactggtgat cgatttgtcg gcgcgcgcga taaaagtata gagaatgggc aatacgccat
     5761 tgaggttttg ctcgtgcaga tcgattttca tgtttttggt gatgaagtag gtggtgctga
     5821 gcgaggactt catcgcgttc tccaggttcc cgagcgcgcc ggagagattt gggatgcgga
     5881 gcggatcggg cggcggcccc atcgattctt tcccgcaaag aatatatacc gaggctctcg
     5941 ggaaaaattg atccacgtaa aggaaatcgg ggccgctgaa catataaaac gcgaccggaa
     6001 ttggctgcgt cgcttccgga agataaatgt cttcccaggc gtgcagtttc tggagctggc
     6061 gcacactgag ttttgagaac tctttttcaa agtaggccga atgcgcctgc caggctgggt
     6121 cctgggtgag cggcgcgagt ggagaatctg gcggcaccgg cattccggcg aggaagcgtg
     6181 caatgtcatt gggtccagcg cttaccggga gtgcgcgagg cgcaatctct ggattctcct
     6241 gggcggaaac gccgaacgct cctatcagga tcgacaatgc aatcgtagtg actgcttttc
     6301 cgaaaaagtt ctttttcatg tggagtgacc gtggcgtttt cgggcgcaaa aatagaggag
     6361 caaaccgaaa aacgccgtcc cgagaccagc cagggactct ctgctatgcc agcgcaatag
     6421 ataaaacatc atccaaattg tgattatcga gaagagaatc ggcggcagcg gataagccca
     6481 gactcgatac ggccgcatca ttttcggatg cgtgaagcgg agcac
//
LOCUS       DASQUQ010000275         7829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Vulcanimicrobiaceae bacterium isolate SMAG_U10965
            k141_179037, whole genome shotgun sequence.
ACCESSION   DASQUQ010000275 DASQUQ010000000
VERSION     DASQUQ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946442
            Sequence Read Archive: SRR5918996
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vulcanimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Vulcanimicrobiaceae bacterium
            Bacteria; Bacillati; Vulcanimicrobiota; Vulcanimicrobiia;
            Vulcanimicrobiales; Vulcanimicrobiaceae.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 17:34:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,515
            CDSs (total)                      :: 1,498
            Genes (coding)                    :: 1,490
            CDSs (with protein)               :: 1,490
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Vulcanimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179037"
                     /isolate="SMAG_U10965"
                     /isolation_source="soil"
                     /db_xref="taxon:3107660"
                     /environmental_sample
                     /lat_lon="35.11641 S 141.97633 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..674)
                     /locus_tag="VE486_07550"
     CDS             complement(189..674)
                     /locus_tag="VE486_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HZA38981.1"
                     /translation="MSKPLLIGKLARLAGVTADTIRFYERSGLLPLPHRTTSGYRVYD
                     EAALSQVRFVRKAQSLGFSLDEVRRILSLRGRGPETCRCVIAMAEATLGETGSKLKEL
                     QRFRDGLARNLDRWKRAPKKATAAEFCALIESSASLAADSSRQIEKRRTEMKRHSTKR
                     S"
     gene            897..1148
                     /locus_tag="VE486_07555"
     CDS             897..1148
                     /locus_tag="VE486_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38982.1"
                     /translation="MNTSIFLFVAILTPFSIRSLGRAATLVEHLARVFAQELAVLLLL
                     AAARGRFFSCQVANEFCRSSKRRRSDQRAAKQCDYCFRF"
     gene            complement(1248..2594)
                     /locus_tag="VE486_07560"
     CDS             complement(1248..2594)
                     /locus_tag="VE486_07560"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HZA38983.1"
                     /translation="MKRGDHQEMLIDMRRKWLWTNFTVISLGAWLISSPFTFGYASGS
                     MMWSDVSSGALLVFFSAFALWPRFDFIGRWTVSFVGGWLQFAPLIFWAKSPAAYLNDT
                     LVGVIAITLSILVPMMPGMAHHMEMMKPGPEIPPGWSYNPSSWHQRAPMIALAFIGWL
                     GSRYLAAFQLGYIPHAWDPFFGDSTAKVLTSNVSKMFPISDAGLGAAAYTLEMLMGWM
                     GGTTRWRSMPWMVTFFFILVVPLGIVSIVLIILQPLAVGYWCSICLFTALVMLFMIPL
                     AVDEVIAMGQFLKTSVRNGKSLWHTFWVGGTLDEVNEDKRTPSYGAPISKLIVPMIWG
                     VTAPWNLVMSAAVGIWLMFAPAVFGTQAHAADSDHLIGALIVTISVIVMAEVIRAGRF
                     LNVLLGVWLIIAPWLLTGGSTPAKWNDIIAALAVILINLQRGTVREHYGKWNRLIV"
     gene            complement(2594..3655)
                     /locus_tag="VE486_07565"
     CDS             complement(2594..3655)
                     /locus_tag="VE486_07565"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZA38984.1"
                     /translation="MKQDSKDVVLVTGSSGLIGCAFCKSLAKNYQIIGFDRDGYPFPP
                     PEAECICIDLSSDGSVKHAFERVRYAYGERIASVVHLAAYYSFSGEPSPLYEQITVRG
                     TERMLRELQNFEVEQFVFSSTELVHAATEPGRAINEDSPLDPNWDYPKSKVETENLIQ
                     AQHGNIPIVILRIAGVYTDKCESIPLAHQIQRIYEREITSKLYPGDITHGQAFVHLQD
                     VLDAFQLTIERRRQLPPEATMIIGEPETLSYDELQRTFSRLLHGEEWETREIPKAAAK
                     AGAWLEDHVPFHDTFIKPWMIDRADDHYELDITRARTLLGWEPKHSLRQTLPKMVAAL
                     KAGPAKFYRENELGDPPAE"
     gene            complement(3697..4089)
                     /locus_tag="VE486_07570"
     CDS             complement(3697..4089)
                     /locus_tag="VE486_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZA38985.1"
                     /translation="MASLLRPLLWLSALFYFAEAPLHFFGLPILEHDRIFLPTHDRYI
                     AIMVVTYGVLLILISTNVKKYQALFIITMVGILAQGINAAYIAVTGGYQPYFHTRHLD
                     RAIALIGCGYLLWYPLAWLSWLKARKVA"
     gene            complement(4202..>7829)
                     /locus_tag="VE486_07575"
     CDS             complement(4202..>7829)
                     /locus_tag="VE486_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZA38986.1"
                     /translation="GVYGLLKVPLDAIPDLSDAQVIVYTDWEGRSPDLVEDQITYPIS
                     TVFIAAPKVKFVRGESMFGKSFVYVIFEDGTDIYWARSRVIEYLNSVRGSLPEGVNPT
                     IGPDATGVGWVYEYALVDDSGKHDLAELRSIQDWNLRYALSSVKGVAEVAPVGGFVKQ
                     YQVDLDPNALVAYNIPLSEVVSAIKTSNADVGGKTFEIATTEYFVRGRGYIKNIADIE
                     NIVLKVDKGTPVYVKNVGTVHLGGDIRRGIAELDGKGETVGGIVVMRYGENALNVIDG
                     VKKKIDEIKSSLPAGVRIVPTYDRSELIRRAIATLREKLIEESIVVALVCVVFLWHVR
                     SSLVAIITLPIAIILAFIPMWWMNLTSNIMSLGGIAIAIGAMVDSAIVMIENAHKRLE
                     EARDERAKRRTGETANAQSEQNDTSPTRRFADSPIQLSNAKRIRIIVDAAKSVGRPLF
                     FALLVITVSFIPVFSLTAQEGRLFKPLAFTKTFSMFFAAFLGVTLVPVLMMLLIRGKI
                     TPEIKNPVNRFLIWAYQPLANFVLRYRLFTLACAALLLALTVFPFRRLGSEFMPPLNE
                     GTVLYMPTAVPGISIAEATKILQIQDRQLKKLPETVTVFGKAGQADTPTDPAPLSMFE
                     TVVALKPPDQWRKGMTWDKLLAELDANIKTPGMANIFWMPIQTRTEMLATGFRSVLGV
                     KVFGPDLGQIQKLAVQIEKELADLPNTRSAFAERTVGGYFLDFTVNREAAARYGLKVG
                     DVNDIVESAIGGKNITTTVEGRERYPVNARYARDFRQDIDALKRVLVATPTGAQVPIE
                     SLADIRYKTGPPSVRSENGKLVGFVFVDITTSDIDGYVRQASQKLSQRIQYPPGYYIS
                     WAGQFQYLQAAKERLKIVIPFTLLIIFVLLYMNTKSLVKTAVVLLAVPFSLIGAFWLL
                     FILGYNMSVAVWVGLIALAGLDAETGVVMLLYLDHAWEKFRTEGRMKNMRDLHNAVIE
                     GAVGRVRPKIMTVCAILFGLLPIMWSPAMQAGADVMKRIATPMIGGVVTSAILELLLY
                     PVIYIIWRRRELPEQLEEKEAPLIPPELIPSERTRQRLLKIIALIAAVALILFGGKFV
                     WQKISAPRAEVAQAAPIATQTVNDLTVKLSGQLRNGPSDLLIEFRDAKNQLVDVGDVK
                     MEANMNMPGMQMHAGATVERTTTLGQYRAKIKPEMAGDWMARLSFNGPRGTGQTSFNL
                     NVKQ"
BASE COUNT         1713 a         2155 c         2183 g         1778 t
ORIGIN      
        1 atgggccagc agaatattcg gtgctgctgg cctaataaat ctgccagagg tcagctccga
       61 tcagcggttt gcgatttttc tttgtcatag gcgcatcttc atttcgcagt gacagaaaaa
      121 ctttgctctg ctgtgccggg cggtccggcg aaagaaatct ttgcgctcca atcgccggcc
      181 aataaatttc agcttctttt agtgctatgt cgtttcatct ccgttcttcg cttttcaatc
      241 tgtcgagaag aatctgctgc gaggctcgcg cttgattcga tcaatgcgca aaattccgct
      301 gcagtggctt tcttgggagc gcgtttccag cgatcgagat tacgtgccag cccgtcacgg
      361 aatcgttgca gttccttaag cttggaaccc gtttcaccaa gcgtcgcttc ggccatggca
      421 atgacgcacc ggcaggtttc ggggccgcgt ccgcgaagac tcagaattct gcgcacttcg
      481 tccaggctga acccgagcga ttgcgctttg cggacgaaac ggacttggct tagcgcagcc
      541 tcgtcataaa cgcgataacc actggtcgta cggtgcggca gcgggagcag gccgctccgc
      601 tcatagaagc ggatcgtgtc cgccgttact cccgcaagcc gcgcgagttt tccgattagc
      661 aatggcttcg acacgcgagt tagcgcgcga gcagtgcagc ttgcgcctcg ctgattcggc
      721 cctcaaatac gaggttgccg tctataacaa cggctggcat tgcacgaacg ccgtggcgct
      781 tcgcttcttc gcactgttca tcgccggtgc agcgacgctc gatcacctcg cagtcgcact
      841 gagcgacggc cgttttaacc cgcttgagag tcccgtcgca caacggacaa ccagccgtga
      901 atacttcaat ctttcttttt gtcgccatac ttactccctt ctcgattcgt tcacttggtc
      961 gtgcagcaac acttgtcgag catcttgccc gtgtatttgc acaagagctt gccgttcttt
     1021 tgctgctcgc agcagcacgt ggtcgattct tttcctgtca ggttgcaaac gaattttgtc
     1081 ggagcagcaa gcgccgtcgc tccgatcaac gcgctgccaa gcagtgcgat tactgttttc
     1141 gattttaaca ttctcttcac ctcgctttct gtttagtcaa agcctaagcc ttggagttgt
     1201 gtccaaggtc aaggtctttt ttagaaacac cccaacgcgg ctttggatca tacgattagc
     1261 cggttccatt tcccgtaatg ctcacggaca gttccgcgct gcaaattgat caaaattacg
     1321 gcgagagcgg cgataatgtc gttccacttt gcgggcgttg aaccaccggt gagcagccac
     1381 ggcgcgatga ttagccacac accaagcagc acgttgagga aacggccggc acggatcact
     1441 tcggccatca caattactga gatggtcacg atgagcgcgc cgatgaggtg atcactgtcg
     1501 gccgcgtgag cctgggtacc gaaaaccgcc ggcgcgaaca ttagccagat tccgacagct
     1561 gcgctcatta cgagattcca cggcgccgtg acgccccaga tcatcgggac aatgagcttc
     1621 gagattggcg cgccgtagct cggtgtgcgc ttgtcttcgt tcacttcatc gagcgtgccg
     1681 ccaacccaga atgtgtgcca caatgatttg ccattgcgca cactggtttt gagaaattgc
     1741 cccatcgcga tgacttcgtc gactgcgagc ggaatcatga acagcatcac gagcgcggta
     1801 aataaacaga tgctgcacca atagccgacg gcgagcggtt gaagaatgat cagcacgata
     1861 ctgacgatac caagcggcac gacgagaatg aaaaagaagg tcaccatcca aggcatgctg
     1921 cgccagcgtg tcgtgccgcc catccaaccc attaacattt ccagcgtgta ggcagccgcg
     1981 ccgaggccag cgtcggaaat tgggaacatt ttcgaaacgt tggaggtgag cacttttgcc
     2041 gtgctgtcgc cgaaaaacgg gtcccacgcg tgcggaatgt aacccaattg aaacgccgca
     2101 agataacgcg agccgagcca gccgatgaag gcaagcgcga tcatgggcgc gcgctgatgc
     2161 caggaagagg gattgtaact ccagcccggc ggaatttcgg gacccggttt catcatctcc
     2221 atgtgatgcg ccatccccgg catcatcggc accaggatcg acaatgttat tgcgatcacg
     2281 ccgacaagcg tgtcgttaag ataggcggcg ggcgatttcg cccagaaaat tagcggcgca
     2341 aattgcagcc agccgcccac gaacgaaacg gtccagcgtc caatgaaatc aaagcgtggc
     2401 cagagcgcga acgcagagaa gaacacgagc agcgcgccgc tactcacgtc gctccacatc
     2461 atagagccgc tcgcgtatcc gaatgtgaat gggctactaa tcaaccacgc gcctagcgat
     2521 atgacggtga aattcgtcca tagccatttc ctccgcatgt cgatgagcat ctcctgatga
     2581 tcgccgcgtt tcatcattca gcaggcgggt cgcccagttc gttttcgcgg taaaatttcg
     2641 cgggaccggc tttcagtgcc gcgaccattt tcggtagcgt ttgccggagc gaatgtttcg
     2701 gttcccaacc gagcaatgtt cgggcacgcg tgatgtcgag ttcgtaatga tcgtcggccc
     2761 gatcgatcat ccacggtttg atgaacgtgt cgtgaaacgg gacatgatct tccaaccatg
     2821 cgccggcttt cgcagcggcc ttgggaattt cgcgcgtctc ccactcctcg ccgtgaagga
     2881 ggcgactgaa agttcgttgc agctcatcgt agctcaacgt ctccggctcg ccgatgatca
     2941 tggttgcttc cggcggtaat tgcctccggc gctcaattgt tagttggaat gcgtccagca
     3001 catcctgcaa atgaacgaac gcctggccgt gcgtgatatc gcccggatac agcttgctcg
     3061 tgatttcccg ctcgtagatt cgttgaattt gatgtgcaag cgggatggac tcacacttgt
     3121 ccgtgtaaac cccggcaatg cgcagaatca caatcgggat gttgccgtgc tgggcctgaa
     3181 tcagattttc ggtttcgact ttcgatttcg gataatccca gttcggatcg agcggtgaat
     3241 cttcattgat cgctctgccc ggctcggtcg ctgcatgtac cagttcggtg ctggaaaata
     3301 cgaactgttc gacttcgaag ttttgcagct cgcgcagcat ccgctcggta ccgcggacgg
     3361 tgatttgttc gtaaagcgga ctcggttctc ccgagaatga ataataggcg gcgagatgaa
     3421 cgaccgatgc gatgcgctcg ccgtaagcgt agcggacacg ttcgaacgca tgcttcacgc
     3481 tcccatcgga actcagatcg atgcagatgc attccgcttc cggcggcgga aatggatagc
     3541 cgtcccgatc aaacccgatg atttgataat tctttgccag tgacttgcaa aaggcgcagc
     3601 cgatcaagcc gctgcttcca gtgacgagca cgacatcttt ggagtcctgc ttcatcgaat
     3661 tacgcgtatg tgcgagaccg tgcgcctttc gtaccgttac gccacttttc tggctttcaa
     3721 ccatgaaagc cacgccagtg gataccacaa aagatacccg cagccgatta gcgcaatggc
     3781 acgatccagg tgccgagtgt gaaagtaagg ttgataaccg ccggtcacgg caatgtaggc
     3841 tgcgttgatc ccttgagcca ggattccaac catggtgatt atgaatagag cctgatactt
     3901 tttgacattg gttgaaatca agatcagcaa aacaccgtaa gtaaccacca tgatagcaat
     3961 gtagcgatca tgcgtgggca ggaaaatccg atcgtgttcc aggatcggca gcccgaaaaa
     4021 atgaagggga gcttcggcga agtagaataa ggcgctaagc caaagcaacg gtcgaagcag
     4081 agaggccaca atgataaaat ctgccgcgga tcgttaaaaa aagtcaattg ctatccggct
     4141 ccgtgaaggc aagcggtcgg gagacagtgt gtaatcgttc agtttcatat ttggtctgcg
     4201 ttcattgttt gacgttgaga ttgaaacttg tttggccggt cccgcgtgga ccattgaacg
     4261 aaagccgggc catccagtcg cccgccatct ctggcttaat cttggcgcga tattggccta
     4321 aagttgtggt gcgttcaacc gtcgcaccag cgtgcatttg cattccgggc atgttcatgt
     4381 ttgcctccat tttaacgtcg ccaacatcga caagttgatt tttggcatca cggaattcga
     4441 ttaacaggtc actaggcccg tttcgcagtt gaccggaaag tttcactgtc aggtcattaa
     4501 cagtttgcgt ggcgattggc gctgcctgag cgacctccgc tcttggcgcg gagatttttt
     4561 gccagacaaa ttttccgccg aacaagatca gggcgaccgc ggcgatgagc gcgatgatct
     4621 tcagcaaacg ctgccgtgtg cgttccgatg gaattaattc aggcggaatc aagggcgcct
     4681 ctttttcttc gagctgctct ggcaattcgc gtcgccgcca gatgatgtaa atcaccggat
     4741 aaagcagcaa ttcgaggatc gcgctcgtga caacgccgcc gatcattggc gtggcgatgc
     4801 gtttcatcac gtcggcgccg gcttgcatgg cgggcgacca catgatcggt agcaatccga
     4861 atagaattgc gcacaccgtc atgatctttg gtcggacgcg accgacggcg ccttcgatga
     4921 cggcattgtg caaatcgcgc atgtttttca tccggccttc cgtacggaat ttttcccatg
     4981 cgtggtcgag atagagcagc atgacgacac ctgtctcggc gtcgagccca gccaacgcga
     5041 tgagcccaac ccagacagcg acgctcatgt tgtagccgag gataaatagc agccagaaag
     5101 cgccaataag cgagaacggg acggcgagta acacgactgc agttttcacc aacgatttcg
     5161 tgttcatgta aagtagcacg aaaatgatca gcagcgtgaa cgggatcacg atcttgagcc
     5221 gctccttggc cgcttgcaga tattggaact ggcctgccca cgaaatgtaa taacccggcg
     5281 gatactgaat ccgctggctg agcttttgcg acgcctggcg aacgtagccg tcgatatcgc
     5341 ttgtggtgat gtccacgaaa acgaacccga ccaattttcc gttctcgctg cgcacggagg
     5401 gaggaccggt cttgtaacga atgtcggcaa gggattcgat cgggacttgt gcgccggtgg
     5461 gggtggcaac gagcacacgt ttgagtgcgt cgatgtcctg ccgaaaatcg cgcgcgtagc
     5521 gtgcgttcac cgggtaacgc tcgcgcccct cgacggtggt cgtaatgttt ttgccgccga
     5581 tagcagactc gacgatatcg ttcacgtcgc caacttttaa accgtatcgc gcggcggctt
     5641 cgcggttgac agtgaagtcg agaaaatagc cgcccacggt ccgctcagcg aaagcgcttc
     5701 gcgtatttgg caaatcggcg agttcttttt cgatttgcac ggccagcttt tggatctgcc
     5761 cgagatcggg gccgaaaact tttacaccga gcacgctgcg gaaaccggtg gcgagcattt
     5821 cggtgcgcgt ctggatcggc atccagaaaa tgttcgccat gccgggcgtc ttgatgttgg
     5881 cgtctagctc agcaagcagc ttgtcccagg tcattccctt tcgccactga tcgggcggct
     5941 tgagcgcgac aacggtttcg aacatcgaca gcggcgcggg atcagtgggc gtgtctgcct
     6001 ggcccgcttt gccgaatacg gtgaccgttt cgggaagttt tttgagctgg cgatcttgga
     6061 tttgcagaat tttcgtagct tcggcgatgg atatccccgg cacagccgtg ggcatgtaaa
     6121 gcaccgtgcc ttcgttaagg ggtggcatga attcgctgcc aagtctcctg aacggaaaaa
     6181 ccgtaagcgc gagaaggagc gcggcacacg ccagcgtaaa caggcgatag cgcagcacga
     6241 aattcgcgag cggttgatac gcccagatca agaatcgatt gactggattt ttgatctcgg
     6301 gtgtgatttt gccacggatc aagagcatca tcagcacggg aaccagtgtc acgccgagga
     6361 atgccgcgaa gaacatcgaa aaagttttgg tgaacgcgag cggcttgaag agccggccct
     6421 cctgggcggt gagcgagaat actggaatga agctcaccgt gataacgagc agagcgaaga
     6481 aaagtggacg gccgacggac ttggcagcat cgacgatgat gcgaatacgc ttggcattgc
     6541 tgagttgaat cggcgaatcg gcgaatcggc gagtcggcga ggtgtcgttc tgctcgcttt
     6601 gcgcattcgc cgtttcgccc gttcgccgtt tcgccctttc gtctcgcgcc tcttcaaggc
     6661 gcttgtgcgc gttctcgatc attacgatcg cggaatcgac catggcgcca atggcgatgg
     6721 cgatcccacc taacgacatg atgttcgagg tcagattcat ccaccacatt gggatgaacg
     6781 ccaggatgat cgcgatgggc agcgtgataa tcgccaccag tgaggagcgc acgtgccaga
     6841 gaaaaaccac gcagacaagc gcgaccacga tgctttcttc gatcagtttt tcgcgcagcg
     6901 tcgcgatggc gcgccggatc aactcgctgc ggtcgtaagt cggcacgatg cgcacaccgg
     6961 ccggcaggga cgatttgatc tcgtcgattt tcttttttac gccgtcgatg acgttgagtg
     7021 cgttctcgcc atagcgcatc acgacgattc caccgacggt ctcacctttt ccgtccagtt
     7081 ccgcgatccc gcgccggata tcgccgccga gatgaacggt gccgacgttc ttcacgtaaa
     7141 ctggcgtccc tttatcgact ttcagcacga tgttttcgat gtcggcgatg tttttgatgt
     7201 agccccggcc gcgcacgaaa tattccgtgg tcgcgatctc gaaagtcttg ccgccgacgt
     7261 cggcgttgct cgttttgatc gcgctgacga cttcgctgag cgggatgttg taggcgacga
     7321 gcgcgtttgg atcgaggtcg acttggtatt gctttacaaa cccaccgact ggcgcgactt
     7381 ccgcgacccc tttcaccgaa gagagcgcat agcgcagatt ccaatcctga atggaacgca
     7441 gctcggccag atcatgtttg ccgctatcgt cgaccagcgc gtattcgtaa acccagccga
     7501 cgcctgtcgc gtcggggccg atcgtcgggt tcacgccttc cggcagcgag ccgcgcaccg
     7561 aattcaaata ttcaataacg cgcgatcgcg cccaataaat gtctgtgccg tcctcgaaga
     7621 tgacgtaaac gaacgatttg ccgaacattg attcgccacg cacgaatttt actttggggg
     7681 cggcgatgaa gacggtggaa atcggataag tgatttggtc ctcgacaaga tcgggcgaac
     7741 gtccctccca atctgtatag acgatcacct gtgcgtcgct caggtcggga atcgcatcga
     7801 gtggaacttt gagcagtccg taaacgccg
//
